PIEZO2 cDNA ORF clone, Bos taurus(cattle)

The following PIEZO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIEZO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa32648 XM_003587787.5
Latest version!
Bos taurus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa32648 XM_003583977.4
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OBa32648 XM_003587787.4 Bos taurus piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa32648
    Clone ID Related Accession (Same CDS sequence) XM_003587787.4 , XM_003587787.5 , XM_003583977.4
    Accession Version XM_003587787.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587787.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCTCGG AAGTGGTGTG CGGGCTCGTC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCCTGCG CCTTCCGGTA CAACGGGCTC TCCTTCGTCT ACCTCATCTA CCTCCTGCTC
    ATCCCCCTGT TCTCGGAACC AACTAAAGCT ACCATGCAAG GACACACGGG ACGGTTGTTA
    AAGTCTCTGT GCTTCACCAG TCTCTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG
    TTGGCGAGTC TGGAGGCACA GCACCGCATC GCACCTGGTT ACAACTGCTC AACCTGGGAG
    AAGACTTTCC GACAAATCGG CTTCGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC
    CGAGTGTTTG TGCCTGACGT GGGCATGTTC ATCGCCAGCC TGACCATCTG GCTGGTCTGT
    AGGAACATTG TTCAGAAGCC AGTGGCCGAA GAGGCCGCGC AGTGTAACCC GGAATTTGAG
    AACGAAGAAT CGGCTGGAGG AGAAAAAATA GACTCCGAAG AGGCCCTGAT CTATGAAGAG
    GACTTAGATG AAGGAGACGG TGCTGAAGGC GATTTGGAGG AAAGCGCGAA ATTAAAACTC
    TTCCGCAGGC TGGCCTCTGT GGCCTCCAAG CTGAAGGAGT TTATTGGCAA CATGATAACC
    ACTGCGGGGA AGGTTGTTGT GACGATTTTA CTGGGTTCAT CAGGGATGAT GCTGCCCTCT
    CTGACCTCGT CCGTGTACTT CTTCGTGTTT CTGGGCCTGT GCACTTGGTG GTCCTGGTGC
    CGGACCTTTG ACCCCTTGCT GTTCAGCTGT CTCTGCGTCC TGCTGGCCAT CTTCACCGCG
    GGACACCTGA TCGGACTCTA TCTGTACCAG TTCCAGTTCT TCCAGGAAGC CGTTCCCCCC
    AACGACTACT ATGCCAGGTT GTTTGGTATC AAATCAGTCA TTCAGACAGA CTGTTCCAGC
    ACATGGAAGA TCATTGCCAA CCCCGACCTG TCCTGGTACC ACCATGCCAA CCCCATTGTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACC CTGATCCGAA TCTGGCTGCA GGAGCCCGTT
    GTGCAGGAAG AGAGGACCAA GGAGGAGGAC AGAGCCCTGG CCTGCAGCCC TGCGCCCATC
    ACGGCCGAGA GGAGGCGGAG CCTGTGGTAT GCGACCCACT ACCCCACGGA CGAGAGGAAA
    CTTTTATCCA TGACTCAGGA TGACTACAAG CCGTCCGACG GCCTGCTGGT GACCGTGAAC
    GGCAACCCCG TGGACTACCA CACTATCCAC CCCAGCCTCC CGCTGGAGAA CGGCCCCGCC
    AAGGCTGACC TGTACTCCAC GCCGCAGTAC CGCTGGGAGC CTGCCGCTGC CTCCCCCGAA
    AAGAAGGAGG AAGACGATGA TGAGAAAGAA GAGTTTGAGG AGGAGCGGAG CCGGGAGGAG
    AAGAGGAGTG TCAAAGTGCA CGCCATGGTG TCTGTGTTCC AGTTCATCAT GAAGCAGAGC
    TACATCTGTG CCCTTATCGC CATGATGGCC TGGAGCATCA CCTACCACAG CTGGCTGACC
    TTTGTGCTGC TCATCTGGTC CTGCACTCTG TGGATGATCC GCAACCGGAG GAAGTATGCT
    ATGATCAGCT CCCCCTTCAT GGTTGTCTAT GGAAACCTGC TGCTCATACT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA AATTAAAAAG GTCCCAGGTT TCTTAGAAAA GAAGGAGCCG
    GGGGAGCTCG CCTCAAAGAT TCTGTTCACC ATCACATTCT GGCTGCTGCT GAGACAGCAC
    CTCACAGAGC AGAAAGCTCT CCAAGAAAAG GAAGCTCTTC TGTCTGAGGT CAAAATCGGA
    AGTCAGGAAA GAGAAGAAAA AGATGATGAA CAGGACTTAC AGGTGGACAG TGAGGCTCAG
    GAGAAGGAGG AAGAAGAGGA GCCGAAGGAG GAGCAGCAGG AGCGGAGAGA GGAAGAGGAA
    GAGGAAGAAG CAGATGAGCA GGACGTGATG AAGGTGCTGG GGAACCTGGT GGTGGCCCTG
    TTCATCAAGT ACTGGATCTA CGTCTGCGGG GGGATGTTCT TCTTCGTCAG CTTTGAGGGT
    AAAATCGTGA TGTACAAGAT CATCTACATG GTGCTCTTCC TCTTCTGTGT GGCCCTGTAT
    CAGGTGCACT ATGAATGGTG GAGGCGGATC CTGAAGTACT TCTGGATGTC AGTGGTTATC
    TACACGATGC TGGTGCTCAT CTTTATATAC ACATATCAGT TTGAAAACTT CCCAGGCTTG
    TGGCAAAACA TGACCGGACT GAAAAAAGAA AAGCTGGAGG ACCTTGGCCT GAAGCAGTTC
    ACGGTGGCCG AGCTGTTCAC GCGCATCTTC ATCCCTACCT CCTTCCTCCT GGTCTGCATC
    CTGCACCTGC ACTACTTCCA CGACCGGTTC CTGGAGCTCA CCGACCTGAA GTCCATCCCC
    AGCAAGGAGG ACAGCGCCAT CTATAGGCTG GCCCACCCCG AGGGAAGCCT TCCGGACCTT
    GCCATGATGA AGCTGACCGC TAGCCTGGAG GTGGCGGAGG TCAAGCAGCT GGCTGGGCCT
    GGGGAGGAGA TGCCGGGGGG ATGCCCTGCA AAAGCCAAGA AGGGCGAGCC TGGCAGGGAC
    AGCGAGGATG AGGATGAGGA TGAGGACGAA GAGGAGGAGG AGACGTCAGA TCTGAGGAAC
    AAGTGGCACT TGGTGATCGA CCGCCTCACC GTGCTGCTCC TGCGGTTCCT GGAGGGCTTC
    CACCAGCTGC AGACGTTCCT GTGGTGGATC CTGGAGCTGC ACATCGTCAA GATCGTGTCC
    TCCTACATCA TCTGGGTGTC CGTGAAGGAG GTGTCTCTGT TCAACTACGT GTTTCTTATT
    TCCTGGGCTT TTGCTCTGCC GTATGCCAGG CTCCGCCGTC TGGCCTCCAG TGTCTGCACC
    GTCTGGACAT GTGTGATCAT CGTCTGTAAG ATGCTGTACC AGCTGCAGAC CATCAAGCCT
    GAGAACTTCT CTGTGAACTG TTCGTTGCCA AATGAAAACC AGACGAACAT CCCCCTGCAC
    CAGCTGAACA AGTCTCTGCT CTACAGCGCA CCCATTGACC CCACAGAATG GGTGGGCCTC
    AGGAAGTCAT CTCCTCTACT TGTCTACCTG AGGAACAACC TGCTGATGCT GGCCATCCTG
    GCCTTCGAGG TCACCGTCTA CCGCCATCAG GAGTACTACC GAGGTCGGAA CAACCTCTCA
    ACCCCTGTGT CTAAAACCAT CTTCCACAAC ATCACAAGAC TCCAGCTGGA TGATGGACTT
    GTTAACTGTG CCAAGTATTT CATCAATTAC TTCTTCTACA AGTTTGGTCT GGAGACCTGT
    TTCCTCATGT CAGTTAACGT GATCGGCCAG CGCATGGATT TCTACGCCAT GATTCACGCC
    TGCTGGCTGA TTGCCGTCCT GTACCGGCGC AGGAGAAAGG CCATCGCCGA GATCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTCATCTG CATCGGCATC
    CCCCCTGCCC CCTGCCGAGA CTACCCCTGG AGGTTCAAGG GCGCCAGCTT CAATGACAAC
    ATCGTCAAGT GGCTGTACTT CCCTGACTTC ATCGTTCGGC CCAACCCTGT GTTCCTCGTG
    TATGATTTCA TGCTACTCCT GTGTGCCTCG CTACAACGGC AGATCTTTGA GGATGAGAAT
    AAGGCTGCCG TAAGGATCGT GGCAGGTGAC AACGTGGAGA TCTGCATGAA CCTGGACGCG
    GCCTCATTCA GCCAGCACAA TCCCGTGCCC GACTTCATCC ACTGCAGGTC GTATCTGGAC
    ATGTCCAAGG TGGTCATCTT CAGCTACCTC TTCTGGTTCG TGCTCACTAT CATCTTCATC
    ACTGGGACGA CCCGCATCAG CATCTTTTGC ATGGGCTACC TGGTGGCCTG CTTCTACTTC
    CTGCTCTTCG GGGGCGACTT GCTGCTGAAA CCCATCAAGA GCATCCTGCG CTACTGGGAC
    TGGCTGATCG CTTACAACGT CTTTGTGATC ACGATGAAAA ACATACTGTC GATAGGAGCA
    TGTGGCTATA TTGAAAAATT GGTTCAAAAT AGTTGCTGGT TGATCCAAGC ATTCAGCCTG
    GCCTGTACAG TCAAGGGCTA CAAAATGCCT GATGATGACT CCTCCTGTAA ACTGCCCAGT
    GGGGAGGCGG GAATCATTTG GGACAGCATA TGCTTTGCCT TCCTGCTGCT GCAGAGAAGA
    GTGTTCATGA GTTACTATTT CCTGCACGTG GTGGCAGATA TAAAAGCCTC GCAGATCCTG
    GCATCCAGAG GGGCTGAGCT TTTCCAGGCC ACCATTGTGA AGGCTGTGAA GGCCAGAATC
    GAGGAGGAGA AAAAGTCCAT GGACCAGCTG AAGAGGCAGA TGGACCGCAT CAAGGCCAGG
    CAACAGAAAT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCGGGGGAA
    GGCCAAGATC TCCAAAAAGT TTCTGAAGAG GATGACGAGA GAGAAGCAGA CAAACAGAAA
    ACCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ACTATTTGTT TGAAACGGAT AGTGAAGAGG AAGAAGAGGA AGAATTAAAA
    AAGGAAGACG AAGAGCCCCC CCGGAGGTCA GCTTTCCAGT TTGTTTATCA AGCCTGGATT
    ACTGACCCTA AAACAGCACT CCGACAGAGG CGCAAAGAGA AAAAAAGATC TGCAAGAGAA
    GAACAGAAAA GAAGGAGAAA AGAATCCGGG GAGGGTCCTG TGGAGTGGGA AGACCGGGAG
    GATGAGCCAG TCAAAAAGAA GTCTGATGGA CCAGACAACA TCATCAAGAG GATCTTTAAT
    ATTTTGAAGT TCACCTGGGT CCTGTTTCTG GCAACCGTGG ACAGTTTCAC AACGTGGCTC
    AACTCCATCT CCAGGGAGCA CCTCGACATC TCCACTGTCC TGAGGATCGA GCGCTGCATG
    CTGACCAGAG AGATCAAGAA GGGCAACGTC CCAACCCGGG AGAGCATCCA CATGTACTAC
    CAGAACCACA TCATGAACCT GTCCAGGGAG TCGGGACTGG ACACCATCGA CGAGCACGCT
    GGCACTGCTG CAGGTGCCCA GACTGCCCAC AGGATGGACA GCTTGGATTC CCACGACAGC
    ATCTCCAGCG AGCCCACGCA GTGCACGATG CTGTACTCGC GCCAGGGGAC CACGGAGACC
    ATCGAGGAAG TGGAGGCTGA GCAGGACGAG GAGGCGCCCG AGGGGGCCCG GGAGGCTGAG
    TCCCCACAGG ATCCCACGGA GGATGGTGAC GTGGAGGCCC CGCCATCCTA CAGCAAGGCT
    GTCAGCTTTG AGCGCCTGTC CTTCGACTCC CGGGACGACT CAGCCGGCCA GACCCACATG
    GAAGTCAGCC CGGATGACAG CCGCTCGGAT CGGCTGGAGG CCAGCATCCT GCCACCTCTG
    ACCCATGAGC TGACAGCCAG TGAGCTGCTG CTGAACAAGA TGTTCCATGA CGACGAGCTG
    GAGGAGTCAG AGAAGTTCTA CGTGGGGCAG CCTCGCTTCC TGCTGCTCTT CTACGCACTG
    TACAACACGC TGGTGGCCCG CTCTGAGATG GTGTGCTACT TTGTCATCAT CCTCAACCAC
    ATGGTCTCCG CCTCCATGAT TACCCTCGTG CTGCCCATCC TTATCTTCCT CTGGGCCATG
    CTGTCCGTCC CCCGGCCCAG CAGGCGCTTC TGGATGATGG CCATCGTCTA CACGGAGGTG
    GCCATTGTGG TCAAGTACTT CTTCCAATTT GGGTTCTTCC CCTGGAACAA GAATGTGGAA
    CTGAACAAGG ACAAGCCTTA CCACCCACCC AACATCATCG GGGTGGAGAA GAAGGACGCC
    TACGTGCTCT ACGACCTCAT CCAGCTGCTG GCGCTCTTCT TCCACCGCTC CATCCTAAAG
    TGTCACGGCC TCTGGGATGA AGACGACATC GTGGACAGCG GCTCAGACAA AGTGGACTCA
    GACGACGAGC TGTCCCTCGG CCATGGCAGG AGGGCCTCCT CCGACTCGCT CAAGTCCATC
    AACCTGGCCG CGTCCGTGGA GTCAGTGCAC GTGACCTTCC CCGAGCAGCC GGCTGCCGTC
    CGGAGGAAGC GCTCCAGCAG CGGCTCCCAG ACATCACAGG GGTCCAGCTT TTCCTCGAAT
    AGGTCCAAGA GAGGCAGCAC GAGCACCCGG AACAGCAGCC AGAAAGGAAG CAGCGTCGTG
    AGCGTCAAGC AGAAGAGCAG ACGGGAACTT TACATGGAGA AGTTGCAGGA CCACTTAATC
    AAAGCAAAAG CCTTTACCAT AAAGAAGACC CTGCAGCTCT ACGTGCCCAT CAGGCAGTTC
    TTCTACGACC TCATCCACCC CGACTACAGC GCCGTCACCG ATGTGTACGT GCTTATGTTC
    CTGGCGGACA CTGTGGACTT CGTCATCATT GTCTTCGGCT TCTGGGCCTT CGGGAAACAC
    TCGGCTGCGG CCGACATCAC GTCCTCGCTG TCCGAGGACC AGGTGCCGGG GCCCTTCCTG
    GTGATGGTGC TCATTCAGTT CGGGACCATG GTGGTGGACC GGGCGCTCTA CCTCAGGAAG
    ACTGTCCTAG GAAAGGTCAT CTTCCAGGTC ATCCTGGTGT TTGGGATTCA CTTCTGGATG
    TTCTTCATCC TGCCTGGCGT GACCGAGAGG AAGTTCAGCC AGAACCTGGT TGCGCAGCTG
    TGGTACTTCG TCAAGTGCGT GTACTTCGGG CTGTCCGCCT ACCAGATCCG CTGCGGCTAC
    CCAACTCGGG TCCTCGGCAA CTTCCTCACC AAGAGCTACA ACTACGTCAA CCTCTTCCTG
    TTCCAGGGGT TCCGCCTTGT CCCTTTCCTG ACGGAGCTGA GGGCTGTGAT GGACTGGGTG
    TGGACAGACA CGACCCTGAG CCTGTCCAGC TGGATCTGTG TGGAGGACAT CTATGCTCAC
    ATCTTCATCC TCAAGTGTTG GCGGGAGTCG GAAAAAAGGT ACCCGCAGCC CCGGGGGCAG
    AAGAAGAAGA AGGTGGTCAA GTACGGGATG GGGGGCATGA TCATCGTGCT GCTCATCTGC
    ATCGTCTGGT TCCCGCTACT CTTCATGTCT CTGGTCAAAT CCGTCGCTGG GGTTATCAAC
    CAGCCCCTGG ACGTCTCAGT CACAATCACC CTGGGCGGGT ACCAGCCTAT TTTCACAATG
    AGTGCCCAGC AAAGCCAGCT GAAAGTTATG GACCAGCCAA TGTTTAATAA ATTCATTAAA
    GCTTTTTCTA AGGACACTGG TGCTATGCAA TTTCTGGAAA ATTATGAAAA AGAAGACATA
    ACAGTCGCAG AACTGGAAGG AAACTCAAAT TCTTTATGGA CCATCAGCCC TCCCAGTAAG
    CAGAAAATGA TACACGAACT CAAGGACCCC AATAGTAGCT TCTCTGTTGT TTTTTCATGG
    AGTATTCAGA GAAACATGAG TCTGGGTGCA AAAGCAGAAA TAGCAACAGA TAAGCTTTCT
    TTCCCTCTTA AAAACGCGAC ACGAGAGAAT ATTGCTAAAA TGATAGCTGG CGGCGACACG
    GAAAGCTCCA GGACGCCCGT GACCATAGAA AGGATTTATC CATACTACGT GAAAGCACCC
    AGTGACTCTA ACTCCAAACC CATAAAGCAG CTTTTATCTG AAAATAATTT CATGAACATC
    ACCATCATTT TGTCCAGAGA CAATGCGACT AACTCGAACA GTGAGTGGTG GGTTCTCAAC
    TTGACTGGGA ACAGAATCTA CAACCAGGAA GCCCAGGCCC TGGAGCTGGT GGTCTTCAAC
    GACAAGGTCA GCCCCCCCAG CCTGGGGTTC CTGGCCGGCT ATGGTATCAT GGGCCTTTAC
    GCTTCCGTGG TGCTGGTGAT TGGGAAGTTT GTCCGGGAAT TCTTCAGCGG GATTTCTCAC
    TCCATCATGT TTGAAGAACT TCCCAACGTG GATCGGATTC TGAAATTGTG CACAGACATT
    TTTTTAGTCC GGGAGACCGG GGAGCTGGAG CTCGAGGAGG ATCTCTATGC CAAATTAATA
    TTCCTGTACC GGTCGCCAGA AACCATGATC AAATGGACCA GGGAGAAAAC AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587835.2
    CDS27..8183
    Translation

    Target ORF information:

    RefSeq Version XM_003587787.4
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587787.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCTCGG AAGTGGTGTG CGGGCTCGTC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCCTGCG CCTTCCGGTA CAACGGGCTC TCCTTCGTCT ACCTCATCTA CCTCCTGCTC
    ATCCCCCTGT TCTCGGAACC AACTAAAGCT ACCATGCAAG GACACACGGG ACGGTTGTTA
    AAGTCTCTGT GCTTCACCAG TCTCTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG
    TTGGCGAGTC TGGAGGCACA GCACCGCATC GCACCTGGTT ACAACTGCTC AACCTGGGAG
    AAGACTTTCC GACAAATCGG CTTCGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC
    CGAGTGTTTG TGCCTGACGT GGGCATGTTC ATCGCCAGCC TGACCATCTG GCTGGTCTGT
    AGGAACATTG TTCAGAAGCC AGTGGCCGAA GAGGCCGCGC AGTGTAACCC GGAATTTGAG
    AACGAAGAAT CGGCTGGAGG AGAAAAAATA GACTCCGAAG AGGCCCTGAT CTATGAAGAG
    GACTTAGATG AAGGAGACGG TGCTGAAGGC GATTTGGAGG AAAGCGCGAA ATTAAAACTC
    TTCCGCAGGC TGGCCTCTGT GGCCTCCAAG CTGAAGGAGT TTATTGGCAA CATGATAACC
    ACTGCGGGGA AGGTTGTTGT GACGATTTTA CTGGGTTCAT CAGGGATGAT GCTGCCCTCT
    CTGACCTCGT CCGTGTACTT CTTCGTGTTT CTGGGCCTGT GCACTTGGTG GTCCTGGTGC
    CGGACCTTTG ACCCCTTGCT GTTCAGCTGT CTCTGCGTCC TGCTGGCCAT CTTCACCGCG
    GGACACCTGA TCGGACTCTA TCTGTACCAG TTCCAGTTCT TCCAGGAAGC CGTTCCCCCC
    AACGACTACT ATGCCAGGTT GTTTGGTATC AAATCAGTCA TTCAGACAGA CTGTTCCAGC
    ACATGGAAGA TCATTGCCAA CCCCGACCTG TCCTGGTACC ACCATGCCAA CCCCATTGTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACC CTGATCCGAA TCTGGCTGCA GGAGCCCGTT
    GTGCAGGAAG AGAGGACCAA GGAGGAGGAC AGAGCCCTGG CCTGCAGCCC TGCGCCCATC
    ACGGCCGAGA GGAGGCGGAG CCTGTGGTAT GCGACCCACT ACCCCACGGA CGAGAGGAAA
    CTTTTATCCA TGACTCAGGA TGACTACAAG CCGTCCGACG GCCTGCTGGT GACCGTGAAC
    GGCAACCCCG TGGACTACCA CACTATCCAC CCCAGCCTCC CGCTGGAGAA CGGCCCCGCC
    AAGGCTGACC TGTACTCCAC GCCGCAGTAC CGCTGGGAGC CTGCCGCTGC CTCCCCCGAA
    AAGAAGGAGG AAGACGATGA TGAGAAAGAA GAGTTTGAGG AGGAGCGGAG CCGGGAGGAG
    AAGAGGAGTG TCAAAGTGCA CGCCATGGTG TCTGTGTTCC AGTTCATCAT GAAGCAGAGC
    TACATCTGTG CCCTTATCGC CATGATGGCC TGGAGCATCA CCTACCACAG CTGGCTGACC
    TTTGTGCTGC TCATCTGGTC CTGCACTCTG TGGATGATCC GCAACCGGAG GAAGTATGCT
    ATGATCAGCT CCCCCTTCAT GGTTGTCTAT GGAAACCTGC TGCTCATACT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA AATTAAAAAG GTCCCAGGTT TCTTAGAAAA GAAGGAGCCG
    GGGGAGCTCG CCTCAAAGAT TCTGTTCACC ATCACATTCT GGCTGCTGCT GAGACAGCAC
    CTCACAGAGC AGAAAGCTCT CCAAGAAAAG GAAGCTCTTC TGTCTGAGGT CAAAATCGGA
    AGTCAGGAAA GAGAAGAAAA AGATGATGAA CAGGACTTAC AGGTGGACAG TGAGGCTCAG
    GAGAAGGAGG AAGAAGAGGA GCCGAAGGAG GAGCAGCAGG AGCGGAGAGA GGAAGAGGAA
    GAGGAAGAAG CAGATGAGCA GGACGTGATG AAGGTGCTGG GGAACCTGGT GGTGGCCCTG
    TTCATCAAGT ACTGGATCTA CGTCTGCGGG GGGATGTTCT TCTTCGTCAG CTTTGAGGGT
    AAAATCGTGA TGTACAAGAT CATCTACATG GTGCTCTTCC TCTTCTGTGT GGCCCTGTAT
    CAGGTGCACT ATGAATGGTG GAGGCGGATC CTGAAGTACT TCTGGATGTC AGTGGTTATC
    TACACGATGC TGGTGCTCAT CTTTATATAC ACATATCAGT TTGAAAACTT CCCAGGCTTG
    TGGCAAAACA TGACCGGACT GAAAAAAGAA AAGCTGGAGG ACCTTGGCCT GAAGCAGTTC
    ACGGTGGCCG AGCTGTTCAC GCGCATCTTC ATCCCTACCT CCTTCCTCCT GGTCTGCATC
    CTGCACCTGC ACTACTTCCA CGACCGGTTC CTGGAGCTCA CCGACCTGAA GTCCATCCCC
    AGCAAGGAGG ACAGCGCCAT CTATAGGCTG GCCCACCCCG AGGGAAGCCT TCCGGACCTT
    GCCATGATGA AGCTGACCGC TAGCCTGGAG GTGGCGGAGG TCAAGCAGCT GGCTGGGCCT
    GGGGAGGAGA TGCCGGGGGG ATGCCCTGCA AAAGCCAAGA AGGGCGAGCC TGGCAGGGAC
    AGCGAGGATG AGGATGAGGA TGAGGACGAA GAGGAGGAGG AGACGTCAGA TCTGAGGAAC
    AAGTGGCACT TGGTGATCGA CCGCCTCACC GTGCTGCTCC TGCGGTTCCT GGAGGGCTTC
    CACCAGCTGC AGACGTTCCT GTGGTGGATC CTGGAGCTGC ACATCGTCAA GATCGTGTCC
    TCCTACATCA TCTGGGTGTC CGTGAAGGAG GTGTCTCTGT TCAACTACGT GTTTCTTATT
    TCCTGGGCTT TTGCTCTGCC GTATGCCAGG CTCCGCCGTC TGGCCTCCAG TGTCTGCACC
    GTCTGGACAT GTGTGATCAT CGTCTGTAAG ATGCTGTACC AGCTGCAGAC CATCAAGCCT
    GAGAACTTCT CTGTGAACTG TTCGTTGCCA AATGAAAACC AGACGAACAT CCCCCTGCAC
    CAGCTGAACA AGTCTCTGCT CTACAGCGCA CCCATTGACC CCACAGAATG GGTGGGCCTC
    AGGAAGTCAT CTCCTCTACT TGTCTACCTG AGGAACAACC TGCTGATGCT GGCCATCCTG
    GCCTTCGAGG TCACCGTCTA CCGCCATCAG GAGTACTACC GAGGTCGGAA CAACCTCTCA
    ACCCCTGTGT CTAAAACCAT CTTCCACAAC ATCACAAGAC TCCAGCTGGA TGATGGACTT
    GTTAACTGTG CCAAGTATTT CATCAATTAC TTCTTCTACA AGTTTGGTCT GGAGACCTGT
    TTCCTCATGT CAGTTAACGT GATCGGCCAG CGCATGGATT TCTACGCCAT GATTCACGCC
    TGCTGGCTGA TTGCCGTCCT GTACCGGCGC AGGAGAAAGG CCATCGCCGA GATCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTCATCTG CATCGGCATC
    CCCCCTGCCC CCTGCCGAGA CTACCCCTGG AGGTTCAAGG GCGCCAGCTT CAATGACAAC
    ATCGTCAAGT GGCTGTACTT CCCTGACTTC ATCGTTCGGC CCAACCCTGT GTTCCTCGTG
    TATGATTTCA TGCTACTCCT GTGTGCCTCG CTACAACGGC AGATCTTTGA GGATGAGAAT
    AAGGCTGCCG TAAGGATCGT GGCAGGTGAC AACGTGGAGA TCTGCATGAA CCTGGACGCG
    GCCTCATTCA GCCAGCACAA TCCCGTGCCC GACTTCATCC ACTGCAGGTC GTATCTGGAC
    ATGTCCAAGG TGGTCATCTT CAGCTACCTC TTCTGGTTCG TGCTCACTAT CATCTTCATC
    ACTGGGACGA CCCGCATCAG CATCTTTTGC ATGGGCTACC TGGTGGCCTG CTTCTACTTC
    CTGCTCTTCG GGGGCGACTT GCTGCTGAAA CCCATCAAGA GCATCCTGCG CTACTGGGAC
    TGGCTGATCG CTTACAACGT CTTTGTGATC ACGATGAAAA ACATACTGTC GATAGGAGCA
    TGTGGCTATA TTGAAAAATT GGTTCAAAAT AGTTGCTGGT TGATCCAAGC ATTCAGCCTG
    GCCTGTACAG TCAAGGGCTA CAAAATGCCT GATGATGACT CCTCCTGTAA ACTGCCCAGT
    GGGGAGGCGG GAATCATTTG GGACAGCATA TGCTTTGCCT TCCTGCTGCT GCAGAGAAGA
    GTGTTCATGA GTTACTATTT CCTGCACGTG GTGGCAGATA TAAAAGCCTC GCAGATCCTG
    GCATCCAGAG GGGCTGAGCT TTTCCAGGCC ACCATTGTGA AGGCTGTGAA GGCCAGAATC
    GAGGAGGAGA AAAAGTCCAT GGACCAGCTG AAGAGGCAGA TGGACCGCAT CAAGGCCAGG
    CAACAGAAAT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCGGGGGAA
    GGCCAAGATC TCCAAAAAGT TTCTGAAGAG GATGACGAGA GAGAAGCAGA CAAACAGAAA
    ACCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ACTATTTGTT TGAAACGGAT AGTGAAGAGG AAGAAGAGGA AGAATTAAAA
    AAGGAAGACG AAGAGCCCCC CCGGAGGTCA GCTTTCCAGT TTGTTTATCA AGCCTGGATT
    ACTGACCCTA AAACAGCACT CCGACAGAGG CGCAAAGAGA AAAAAAGATC TGCAAGAGAA
    GAACAGAAAA GAAGGAGAAA AGAATCCGGG GAGGGTCCTG TGGAGTGGGA AGACCGGGAG
    GATGAGCCAG TCAAAAAGAA GTCTGATGGA CCAGACAACA TCATCAAGAG GATCTTTAAT
    ATTTTGAAGT TCACCTGGGT CCTGTTTCTG GCAACCGTGG ACAGTTTCAC AACGTGGCTC
    AACTCCATCT CCAGGGAGCA CCTCGACATC TCCACTGTCC TGAGGATCGA GCGCTGCATG
    CTGACCAGAG AGATCAAGAA GGGCAACGTC CCAACCCGGG AGAGCATCCA CATGTACTAC
    CAGAACCACA TCATGAACCT GTCCAGGGAG TCGGGACTGG ACACCATCGA CGAGCACGCT
    GGCACTGCTG CAGGTGCCCA GACTGCCCAC AGGATGGACA GCTTGGATTC CCACGACAGC
    ATCTCCAGCG AGCCCACGCA GTGCACGATG CTGTACTCGC GCCAGGGGAC CACGGAGACC
    ATCGAGGAAG TGGAGGCTGA GCAGGACGAG GAGGCGCCCG AGGGGGCCCG GGAGGCTGAG
    TCCCCACAGG ATCCCACGGA GGATGGTGAC GTGGAGGCCC CGCCATCCTA CAGCAAGGCT
    GTCAGCTTTG AGCGCCTGTC CTTCGACTCC CGGGACGACT CAGCCGGCCA GACCCACATG
    GAAGTCAGCC CGGATGACAG CCGCTCGGAT CGGCTGGAGG CCAGCATCCT GCCACCTCTG
    ACCCATGAGC TGACAGCCAG TGAGCTGCTG CTGAACAAGA TGTTCCATGA CGACGAGCTG
    GAGGAGTCAG AGAAGTTCTA CGTGGGGCAG CCTCGCTTCC TGCTGCTCTT CTACGCACTG
    TACAACACGC TGGTGGCCCG CTCTGAGATG GTGTGCTACT TTGTCATCAT CCTCAACCAC
    ATGGTCTCCG CCTCCATGAT TACCCTCGTG CTGCCCATCC TTATCTTCCT CTGGGCCATG
    CTGTCCGTCC CCCGGCCCAG CAGGCGCTTC TGGATGATGG CCATCGTCTA CACGGAGGTG
    GCCATTGTGG TCAAGTACTT CTTCCAATTT GGGTTCTTCC CCTGGAACAA GAATGTGGAA
    CTGAACAAGG ACAAGCCTTA CCACCCACCC AACATCATCG GGGTGGAGAA GAAGGACGCC
    TACGTGCTCT ACGACCTCAT CCAGCTGCTG GCGCTCTTCT TCCACCGCTC CATCCTAAAG
    TGTCACGGCC TCTGGGATGA AGACGACATC GTGGACAGCG GCTCAGACAA AGTGGACTCA
    GACGACGAGC TGTCCCTCGG CCATGGCAGG AGGGCCTCCT CCGACTCGCT CAAGTCCATC
    AACCTGGCCG CGTCCGTGGA GTCAGTGCAC GTGACCTTCC CCGAGCAGCC GGCTGCCGTC
    CGGAGGAAGC GCTCCAGCAG CGGCTCCCAG ACATCACAGG GGTCCAGCTT TTCCTCGAAT
    AGGTCCAAGA GAGGCAGCAC GAGCACCCGG AACAGCAGCC AGAAAGGAAG CAGCGTCGTG
    AGCGTCAAGC AGAAGAGCAG ACGGGAACTT TACATGGAGA AGTTGCAGGA CCACTTAATC
    AAAGCAAAAG CCTTTACCAT AAAGAAGACC CTGCAGCTCT ACGTGCCCAT CAGGCAGTTC
    TTCTACGACC TCATCCACCC CGACTACAGC GCCGTCACCG ATGTGTACGT GCTTATGTTC
    CTGGCGGACA CTGTGGACTT CGTCATCATT GTCTTCGGCT TCTGGGCCTT CGGGAAACAC
    TCGGCTGCGG CCGACATCAC GTCCTCGCTG TCCGAGGACC AGGTGCCGGG GCCCTTCCTG
    GTGATGGTGC TCATTCAGTT CGGGACCATG GTGGTGGACC GGGCGCTCTA CCTCAGGAAG
    ACTGTCCTAG GAAAGGTCAT CTTCCAGGTC ATCCTGGTGT TTGGGATTCA CTTCTGGATG
    TTCTTCATCC TGCCTGGCGT GACCGAGAGG AAGTTCAGCC AGAACCTGGT TGCGCAGCTG
    TGGTACTTCG TCAAGTGCGT GTACTTCGGG CTGTCCGCCT ACCAGATCCG CTGCGGCTAC
    CCAACTCGGG TCCTCGGCAA CTTCCTCACC AAGAGCTACA ACTACGTCAA CCTCTTCCTG
    TTCCAGGGGT TCCGCCTTGT CCCTTTCCTG ACGGAGCTGA GGGCTGTGAT GGACTGGGTG
    TGGACAGACA CGACCCTGAG CCTGTCCAGC TGGATCTGTG TGGAGGACAT CTATGCTCAC
    ATCTTCATCC TCAAGTGTTG GCGGGAGTCG GAAAAAAGGT ACCCGCAGCC CCGGGGGCAG
    AAGAAGAAGA AGGTGGTCAA GTACGGGATG GGGGGCATGA TCATCGTGCT GCTCATCTGC
    ATCGTCTGGT TCCCGCTACT CTTCATGTCT CTGGTCAAAT CCGTCGCTGG GGTTATCAAC
    CAGCCCCTGG ACGTCTCAGT CACAATCACC CTGGGCGGGT ACCAGCCTAT TTTCACAATG
    AGTGCCCAGC AAAGCCAGCT GAAAGTTATG GACCAGCCAA TGTTTAATAA ATTCATTAAA
    GCTTTTTCTA AGGACACTGG TGCTATGCAA TTTCTGGAAA ATTATGAAAA AGAAGACATA
    ACAGTCGCAG AACTGGAAGG AAACTCAAAT TCTTTATGGA CCATCAGCCC TCCCAGTAAG
    CAGAAAATGA TACACGAACT CAAGGACCCC AATAGTAGCT TCTCTGTTGT TTTTTCATGG
    AGTATTCAGA GAAACATGAG TCTGGGTGCA AAAGCAGAAA TAGCAACAGA TAAGCTTTCT
    TTCCCTCTTA AAAACGCGAC ACGAGAGAAT ATTGCTAAAA TGATAGCTGG CGGCGACACG
    GAAAGCTCCA GGACGCCCGT GACCATAGAA AGGATTTATC CATACTACGT GAAAGCACCC
    AGTGACTCTA ACTCCAAACC CATAAAGCAG CTTTTATCTG AAAATAATTT CATGAACATC
    ACCATCATTT TGTCCAGAGA CAATGCGACT AACTCGAACA GTGAGTGGTG GGTTCTCAAC
    TTGACTGGGA ACAGAATCTA CAACCAGGAA GCCCAGGCCC TGGAGCTGGT GGTCTTCAAC
    GACAAGGTCA GCCCCCCCAG CCTGGGGTTC CTGGCCGGCT ATGGTATCAT GGGCCTTTAC
    GCTTCCGTGG TGCTGGTGAT TGGGAAGTTT GTCCGGGAAT TCTTCAGCGG GATTTCTCAC
    TCCATCATGT TTGAAGAACT TCCCAACGTG GATCGGATTC TGAAATTGTG CACAGACATT
    TTTTTAGTCC GGGAGACCGG GGAGCTGGAG CTCGAGGAGG ATCTCTATGC CAAATTAATA
    TTCCTGTACC GGTCGCCAGA AACCATGATC AAATGGACCA GGGAGAAAAC AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32648
    Clone ID Related Accession (Same CDS sequence) XM_003587787.4 , XM_003587787.5 , XM_003583977.4
    Accession Version XM_003587787.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587787.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCTCGG AAGTGGTGTG CGGGCTCGTC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCCTGCG CCTTCCGGTA CAACGGGCTC TCCTTCGTCT ACCTCATCTA CCTCCTGCTC
    ATCCCCCTGT TCTCGGAACC AACTAAAGCT ACCATGCAAG GACACACGGG ACGGTTGTTA
    AAGTCTCTGT GCTTCACCAG TCTCTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG
    TTGGCGAGTC TGGAGGCACA GCACCGCATC GCACCTGGTT ACAACTGCTC AACCTGGGAG
    AAGACTTTCC GACAAATCGG CTTCGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC
    CGAGTGTTTG TGCCTGACGT GGGCATGTTC ATCGCCAGCC TGACCATCTG GCTGGTCTGT
    AGGAACATTG TTCAGAAGCC AGTGGCCGAA GAGGCCGCGC AGTGTAACCC GGAATTTGAG
    AACGAAGAAT CGGCTGGAGG AGAAAAAATA GACTCCGAAG AGGCCCTGAT CTATGAAGAG
    GACTTAGATG AAGGAGACGG TGCTGAAGGC GATTTGGAGG AAAGCGCGAA ATTAAAACTC
    TTCCGCAGGC TGGCCTCTGT GGCCTCCAAG CTGAAGGAGT TTATTGGCAA CATGATAACC
    ACTGCGGGGA AGGTTGTTGT GACGATTTTA CTGGGTTCAT CAGGGATGAT GCTGCCCTCT
    CTGACCTCGT CCGTGTACTT CTTCGTGTTT CTGGGCCTGT GCACTTGGTG GTCCTGGTGC
    CGGACCTTTG ACCCCTTGCT GTTCAGCTGT CTCTGCGTCC TGCTGGCCAT CTTCACCGCG
    GGACACCTGA TCGGACTCTA TCTGTACCAG TTCCAGTTCT TCCAGGAAGC CGTTCCCCCC
    AACGACTACT ATGCCAGGTT GTTTGGTATC AAATCAGTCA TTCAGACAGA CTGTTCCAGC
    ACATGGAAGA TCATTGCCAA CCCCGACCTG TCCTGGTACC ACCATGCCAA CCCCATTGTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACC CTGATCCGAA TCTGGCTGCA GGAGCCCGTT
    GTGCAGGAAG AGAGGACCAA GGAGGAGGAC AGAGCCCTGG CCTGCAGCCC TGCGCCCATC
    ACGGCCGAGA GGAGGCGGAG CCTGTGGTAT GCGACCCACT ACCCCACGGA CGAGAGGAAA
    CTTTTATCCA TGACTCAGGA TGACTACAAG CCGTCCGACG GCCTGCTGGT GACCGTGAAC
    GGCAACCCCG TGGACTACCA CACTATCCAC CCCAGCCTCC CGCTGGAGAA CGGCCCCGCC
    AAGGCTGACC TGTACTCCAC GCCGCAGTAC CGCTGGGAGC CTGCCGCTGC CTCCCCCGAA
    AAGAAGGAGG AAGACGATGA TGAGAAAGAA GAGTTTGAGG AGGAGCGGAG CCGGGAGGAG
    AAGAGGAGTG TCAAAGTGCA CGCCATGGTG TCTGTGTTCC AGTTCATCAT GAAGCAGAGC
    TACATCTGTG CCCTTATCGC CATGATGGCC TGGAGCATCA CCTACCACAG CTGGCTGACC
    TTTGTGCTGC TCATCTGGTC CTGCACTCTG TGGATGATCC GCAACCGGAG GAAGTATGCT
    ATGATCAGCT CCCCCTTCAT GGTTGTCTAT GGAAACCTGC TGCTCATACT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA AATTAAAAAG GTCCCAGGTT TCTTAGAAAA GAAGGAGCCG
    GGGGAGCTCG CCTCAAAGAT TCTGTTCACC ATCACATTCT GGCTGCTGCT GAGACAGCAC
    CTCACAGAGC AGAAAGCTCT CCAAGAAAAG GAAGCTCTTC TGTCTGAGGT CAAAATCGGA
    AGTCAGGAAA GAGAAGAAAA AGATGATGAA CAGGACTTAC AGGTGGACAG TGAGGCTCAG
    GAGAAGGAGG AAGAAGAGGA GCCGAAGGAG GAGCAGCAGG AGCGGAGAGA GGAAGAGGAA
    GAGGAAGAAG CAGATGAGCA GGACGTGATG AAGGTGCTGG GGAACCTGGT GGTGGCCCTG
    TTCATCAAGT ACTGGATCTA CGTCTGCGGG GGGATGTTCT TCTTCGTCAG CTTTGAGGGT
    AAAATCGTGA TGTACAAGAT CATCTACATG GTGCTCTTCC TCTTCTGTGT GGCCCTGTAT
    CAGGTGCACT ATGAATGGTG GAGGCGGATC CTGAAGTACT TCTGGATGTC AGTGGTTATC
    TACACGATGC TGGTGCTCAT CTTTATATAC ACATATCAGT TTGAAAACTT CCCAGGCTTG
    TGGCAAAACA TGACCGGACT GAAAAAAGAA AAGCTGGAGG ACCTTGGCCT GAAGCAGTTC
    ACGGTGGCCG AGCTGTTCAC GCGCATCTTC ATCCCTACCT CCTTCCTCCT GGTCTGCATC
    CTGCACCTGC ACTACTTCCA CGACCGGTTC CTGGAGCTCA CCGACCTGAA GTCCATCCCC
    AGCAAGGAGG ACAGCGCCAT CTATAGGCTG GCCCACCCCG AGGGAAGCCT TCCGGACCTT
    GCCATGATGA AGCTGACCGC TAGCCTGGAG GTGGCGGAGG TCAAGCAGCT GGCTGGGCCT
    GGGGAGGAGA TGCCGGGGGG ATGCCCTGCA AAAGCCAAGA AGGGCGAGCC TGGCAGGGAC
    AGCGAGGATG AGGATGAGGA TGAGGACGAA GAGGAGGAGG AGACGTCAGA TCTGAGGAAC
    AAGTGGCACT TGGTGATCGA CCGCCTCACC GTGCTGCTCC TGCGGTTCCT GGAGGGCTTC
    CACCAGCTGC AGACGTTCCT GTGGTGGATC CTGGAGCTGC ACATCGTCAA GATCGTGTCC
    TCCTACATCA TCTGGGTGTC CGTGAAGGAG GTGTCTCTGT TCAACTACGT GTTTCTTATT
    TCCTGGGCTT TTGCTCTGCC GTATGCCAGG CTCCGCCGTC TGGCCTCCAG TGTCTGCACC
    GTCTGGACAT GTGTGATCAT CGTCTGTAAG ATGCTGTACC AGCTGCAGAC CATCAAGCCT
    GAGAACTTCT CTGTGAACTG TTCGTTGCCA AATGAAAACC AGACGAACAT CCCCCTGCAC
    CAGCTGAACA AGTCTCTGCT CTACAGCGCA CCCATTGACC CCACAGAATG GGTGGGCCTC
    AGGAAGTCAT CTCCTCTACT TGTCTACCTG AGGAACAACC TGCTGATGCT GGCCATCCTG
    GCCTTCGAGG TCACCGTCTA CCGCCATCAG GAGTACTACC GAGGTCGGAA CAACCTCTCA
    ACCCCTGTGT CTAAAACCAT CTTCCACAAC ATCACAAGAC TCCAGCTGGA TGATGGACTT
    GTTAACTGTG CCAAGTATTT CATCAATTAC TTCTTCTACA AGTTTGGTCT GGAGACCTGT
    TTCCTCATGT CAGTTAACGT GATCGGCCAG CGCATGGATT TCTACGCCAT GATTCACGCC
    TGCTGGCTGA TTGCCGTCCT GTACCGGCGC AGGAGAAAGG CCATCGCCGA GATCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTCATCTG CATCGGCATC
    CCCCCTGCCC CCTGCCGAGA CTACCCCTGG AGGTTCAAGG GCGCCAGCTT CAATGACAAC
    ATCGTCAAGT GGCTGTACTT CCCTGACTTC ATCGTTCGGC CCAACCCTGT GTTCCTCGTG
    TATGATTTCA TGCTACTCCT GTGTGCCTCG CTACAACGGC AGATCTTTGA GGATGAGAAT
    AAGGCTGCCG TAAGGATCGT GGCAGGTGAC AACGTGGAGA TCTGCATGAA CCTGGACGCG
    GCCTCATTCA GCCAGCACAA TCCCGTGCCC GACTTCATCC ACTGCAGGTC GTATCTGGAC
    ATGTCCAAGG TGGTCATCTT CAGCTACCTC TTCTGGTTCG TGCTCACTAT CATCTTCATC
    ACTGGGACGA CCCGCATCAG CATCTTTTGC ATGGGCTACC TGGTGGCCTG CTTCTACTTC
    CTGCTCTTCG GGGGCGACTT GCTGCTGAAA CCCATCAAGA GCATCCTGCG CTACTGGGAC
    TGGCTGATCG CTTACAACGT CTTTGTGATC ACGATGAAAA ACATACTGTC GATAGGAGCA
    TGTGGCTATA TTGAAAAATT GGTTCAAAAT AGTTGCTGGT TGATCCAAGC ATTCAGCCTG
    GCCTGTACAG TCAAGGGCTA CAAAATGCCT GATGATGACT CCTCCTGTAA ACTGCCCAGT
    GGGGAGGCGG GAATCATTTG GGACAGCATA TGCTTTGCCT TCCTGCTGCT GCAGAGAAGA
    GTGTTCATGA GTTACTATTT CCTGCACGTG GTGGCAGATA TAAAAGCCTC GCAGATCCTG
    GCATCCAGAG GGGCTGAGCT TTTCCAGGCC ACCATTGTGA AGGCTGTGAA GGCCAGAATC
    GAGGAGGAGA AAAAGTCCAT GGACCAGCTG AAGAGGCAGA TGGACCGCAT CAAGGCCAGG
    CAACAGAAAT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCGGGGGAA
    GGCCAAGATC TCCAAAAAGT TTCTGAAGAG GATGACGAGA GAGAAGCAGA CAAACAGAAA
    ACCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ACTATTTGTT TGAAACGGAT AGTGAAGAGG AAGAAGAGGA AGAATTAAAA
    AAGGAAGACG AAGAGCCCCC CCGGAGGTCA GCTTTCCAGT TTGTTTATCA AGCCTGGATT
    ACTGACCCTA AAACAGCACT CCGACAGAGG CGCAAAGAGA AAAAAAGATC TGCAAGAGAA
    GAACAGAAAA GAAGGAGAAA AGAATCCGGG GAGGGTCCTG TGGAGTGGGA AGACCGGGAG
    GATGAGCCAG TCAAAAAGAA GTCTGATGGA CCAGACAACA TCATCAAGAG GATCTTTAAT
    ATTTTGAAGT TCACCTGGGT CCTGTTTCTG GCAACCGTGG ACAGTTTCAC AACGTGGCTC
    AACTCCATCT CCAGGGAGCA CCTCGACATC TCCACTGTCC TGAGGATCGA GCGCTGCATG
    CTGACCAGAG AGATCAAGAA GGGCAACGTC CCAACCCGGG AGAGCATCCA CATGTACTAC
    CAGAACCACA TCATGAACCT GTCCAGGGAG TCGGGACTGG ACACCATCGA CGAGCACGCT
    GGCACTGCTG CAGGTGCCCA GACTGCCCAC AGGATGGACA GCTTGGATTC CCACGACAGC
    ATCTCCAGCG AGCCCACGCA GTGCACGATG CTGTACTCGC GCCAGGGGAC CACGGAGACC
    ATCGAGGAAG TGGAGGCTGA GCAGGACGAG GAGGCGCCCG AGGGGGCCCG GGAGGCTGAG
    TCCCCACAGG ATCCCACGGA GGATGGTGAC GTGGAGGCCC CGCCATCCTA CAGCAAGGCT
    GTCAGCTTTG AGCGCCTGTC CTTCGACTCC CGGGACGACT CAGCCGGCCA GACCCACATG
    GAAGTCAGCC CGGATGACAG CCGCTCGGAT CGGCTGGAGG CCAGCATCCT GCCACCTCTG
    ACCCATGAGC TGACAGCCAG TGAGCTGCTG CTGAACAAGA TGTTCCATGA CGACGAGCTG
    GAGGAGTCAG AGAAGTTCTA CGTGGGGCAG CCTCGCTTCC TGCTGCTCTT CTACGCACTG
    TACAACACGC TGGTGGCCCG CTCTGAGATG GTGTGCTACT TTGTCATCAT CCTCAACCAC
    ATGGTCTCCG CCTCCATGAT TACCCTCGTG CTGCCCATCC TTATCTTCCT CTGGGCCATG
    CTGTCCGTCC CCCGGCCCAG CAGGCGCTTC TGGATGATGG CCATCGTCTA CACGGAGGTG
    GCCATTGTGG TCAAGTACTT CTTCCAATTT GGGTTCTTCC CCTGGAACAA GAATGTGGAA
    CTGAACAAGG ACAAGCCTTA CCACCCACCC AACATCATCG GGGTGGAGAA GAAGGACGCC
    TACGTGCTCT ACGACCTCAT CCAGCTGCTG GCGCTCTTCT TCCACCGCTC CATCCTAAAG
    TGTCACGGCC TCTGGGATGA AGACGACATC GTGGACAGCG GCTCAGACAA AGTGGACTCA
    GACGACGAGC TGTCCCTCGG CCATGGCAGG AGGGCCTCCT CCGACTCGCT CAAGTCCATC
    AACCTGGCCG CGTCCGTGGA GTCAGTGCAC GTGACCTTCC CCGAGCAGCC GGCTGCCGTC
    CGGAGGAAGC GCTCCAGCAG CGGCTCCCAG ACATCACAGG GGTCCAGCTT TTCCTCGAAT
    AGGTCCAAGA GAGGCAGCAC GAGCACCCGG AACAGCAGCC AGAAAGGAAG CAGCGTCGTG
    AGCGTCAAGC AGAAGAGCAG ACGGGAACTT TACATGGAGA AGTTGCAGGA CCACTTAATC
    AAAGCAAAAG CCTTTACCAT AAAGAAGACC CTGCAGCTCT ACGTGCCCAT CAGGCAGTTC
    TTCTACGACC TCATCCACCC CGACTACAGC GCCGTCACCG ATGTGTACGT GCTTATGTTC
    CTGGCGGACA CTGTGGACTT CGTCATCATT GTCTTCGGCT TCTGGGCCTT CGGGAAACAC
    TCGGCTGCGG CCGACATCAC GTCCTCGCTG TCCGAGGACC AGGTGCCGGG GCCCTTCCTG
    GTGATGGTGC TCATTCAGTT CGGGACCATG GTGGTGGACC GGGCGCTCTA CCTCAGGAAG
    ACTGTCCTAG GAAAGGTCAT CTTCCAGGTC ATCCTGGTGT TTGGGATTCA CTTCTGGATG
    TTCTTCATCC TGCCTGGCGT GACCGAGAGG AAGTTCAGCC AGAACCTGGT TGCGCAGCTG
    TGGTACTTCG TCAAGTGCGT GTACTTCGGG CTGTCCGCCT ACCAGATCCG CTGCGGCTAC
    CCAACTCGGG TCCTCGGCAA CTTCCTCACC AAGAGCTACA ACTACGTCAA CCTCTTCCTG
    TTCCAGGGGT TCCGCCTTGT CCCTTTCCTG ACGGAGCTGA GGGCTGTGAT GGACTGGGTG
    TGGACAGACA CGACCCTGAG CCTGTCCAGC TGGATCTGTG TGGAGGACAT CTATGCTCAC
    ATCTTCATCC TCAAGTGTTG GCGGGAGTCG GAAAAAAGGT ACCCGCAGCC CCGGGGGCAG
    AAGAAGAAGA AGGTGGTCAA GTACGGGATG GGGGGCATGA TCATCGTGCT GCTCATCTGC
    ATCGTCTGGT TCCCGCTACT CTTCATGTCT CTGGTCAAAT CCGTCGCTGG GGTTATCAAC
    CAGCCCCTGG ACGTCTCAGT CACAATCACC CTGGGCGGGT ACCAGCCTAT TTTCACAATG
    AGTGCCCAGC AAAGCCAGCT GAAAGTTATG GACCAGCCAA TGTTTAATAA ATTCATTAAA
    GCTTTTTCTA AGGACACTGG TGCTATGCAA TTTCTGGAAA ATTATGAAAA AGAAGACATA
    ACAGTCGCAG AACTGGAAGG AAACTCAAAT TCTTTATGGA CCATCAGCCC TCCCAGTAAG
    CAGAAAATGA TACACGAACT CAAGGACCCC AATAGTAGCT TCTCTGTTGT TTTTTCATGG
    AGTATTCAGA GAAACATGAG TCTGGGTGCA AAAGCAGAAA TAGCAACAGA TAAGCTTTCT
    TTCCCTCTTA AAAACGCGAC ACGAGAGAAT ATTGCTAAAA TGATAGCTGG CGGCGACACG
    GAAAGCTCCA GGACGCCCGT GACCATAGAA AGGATTTATC CATACTACGT GAAAGCACCC
    AGTGACTCTA ACTCCAAACC CATAAAGCAG CTTTTATCTG AAAATAATTT CATGAACATC
    ACCATCATTT TGTCCAGAGA CAATGCGACT AACTCGAACA GTGAGTGGTG GGTTCTCAAC
    TTGACTGGGA ACAGAATCTA CAACCAGGAA GCCCAGGCCC TGGAGCTGGT GGTCTTCAAC
    GACAAGGTCA GCCCCCCCAG CCTGGGGTTC CTGGCCGGCT ATGGTATCAT GGGCCTTTAC
    GCTTCCGTGG TGCTGGTGAT TGGGAAGTTT GTCCGGGAAT TCTTCAGCGG GATTTCTCAC
    TCCATCATGT TTGAAGAACT TCCCAACGTG GATCGGATTC TGAAATTGTG CACAGACATT
    TTTTTAGTCC GGGAGACCGG GGAGCTGGAG CTCGAGGAGG ATCTCTATGC CAAATTAATA
    TTCCTGTACC GGTCGCCAGA AACCATGATC AAATGGACCA GGGAGAAAAC AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587835.2
    CDS1..8157
    Translation

    Target ORF information:

    RefSeq Version XM_003587787.5
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587787.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCTCGG AAGTGGTGTG CGGGCTCGTC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCCTGCG CCTTCCGGTA CAACGGGCTC TCCTTCGTCT ACCTCATCTA CCTCCTGCTC
    ATCCCCCTGT TCTCGGAACC AACTAAAGCT ACCATGCAAG GACACACGGG ACGGTTGTTA
    AAGTCTCTGT GCTTCACCAG TCTCTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG
    TTGGCGAGTC TGGAGGCACA GCACCGCATC GCACCTGGTT ACAACTGCTC AACCTGGGAG
    AAGACTTTCC GACAAATCGG CTTCGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC
    CGAGTGTTTG TGCCTGACGT GGGCATGTTC ATCGCCAGCC TGACCATCTG GCTGGTCTGT
    AGGAACATTG TTCAGAAGCC AGTGGCCGAA GAGGCCGCGC AGTGTAACCC GGAATTTGAG
    AACGAAGAAT CGGCTGGAGG AGAAAAAATA GACTCCGAAG AGGCCCTGAT CTATGAAGAG
    GACTTAGATG AAGGAGACGG TGCTGAAGGC GATTTGGAGG AAAGCGCGAA ATTAAAACTC
    TTCCGCAGGC TGGCCTCTGT GGCCTCCAAG CTGAAGGAGT TTATTGGCAA CATGATAACC
    ACTGCGGGGA AGGTTGTTGT GACGATTTTA CTGGGTTCAT CAGGGATGAT GCTGCCCTCT
    CTGACCTCGT CCGTGTACTT CTTCGTGTTT CTGGGCCTGT GCACTTGGTG GTCCTGGTGC
    CGGACCTTTG ACCCCTTGCT GTTCAGCTGT CTCTGCGTCC TGCTGGCCAT CTTCACCGCG
    GGACACCTGA TCGGACTCTA TCTGTACCAG TTCCAGTTCT TCCAGGAAGC CGTTCCCCCC
    AACGACTACT ATGCCAGGTT GTTTGGTATC AAATCAGTCA TTCAGACAGA CTGTTCCAGC
    ACATGGAAGA TCATTGCCAA CCCCGACCTG TCCTGGTACC ACCATGCCAA CCCCATTGTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACC CTGATCCGAA TCTGGCTGCA GGAGCCCGTT
    GTGCAGGAAG AGAGGACCAA GGAGGAGGAC AGAGCCCTGG CCTGCAGCCC TGCGCCCATC
    ACGGCCGAGA GGAGGCGGAG CCTGTGGTAT GCGACCCACT ACCCCACGGA CGAGAGGAAA
    CTTTTATCCA TGACTCAGGA TGACTACAAG CCGTCCGACG GCCTGCTGGT GACCGTGAAC
    GGCAACCCCG TGGACTACCA CACTATCCAC CCCAGCCTCC CGCTGGAGAA CGGCCCCGCC
    AAGGCTGACC TGTACTCCAC GCCGCAGTAC CGCTGGGAGC CTGCCGCTGC CTCCCCCGAA
    AAGAAGGAGG AAGACGATGA TGAGAAAGAA GAGTTTGAGG AGGAGCGGAG CCGGGAGGAG
    AAGAGGAGTG TCAAAGTGCA CGCCATGGTG TCTGTGTTCC AGTTCATCAT GAAGCAGAGC
    TACATCTGTG CCCTTATCGC CATGATGGCC TGGAGCATCA CCTACCACAG CTGGCTGACC
    TTTGTGCTGC TCATCTGGTC CTGCACTCTG TGGATGATCC GCAACCGGAG GAAGTATGCT
    ATGATCAGCT CCCCCTTCAT GGTTGTCTAT GGAAACCTGC TGCTCATACT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA AATTAAAAAG GTCCCAGGTT TCTTAGAAAA GAAGGAGCCG
    GGGGAGCTCG CCTCAAAGAT TCTGTTCACC ATCACATTCT GGCTGCTGCT GAGACAGCAC
    CTCACAGAGC AGAAAGCTCT CCAAGAAAAG GAAGCTCTTC TGTCTGAGGT CAAAATCGGA
    AGTCAGGAAA GAGAAGAAAA AGATGATGAA CAGGACTTAC AGGTGGACAG TGAGGCTCAG
    GAGAAGGAGG AAGAAGAGGA GCCGAAGGAG GAGCAGCAGG AGCGGAGAGA GGAAGAGGAA
    GAGGAAGAAG CAGATGAGCA GGACGTGATG AAGGTGCTGG GGAACCTGGT GGTGGCCCTG
    TTCATCAAGT ACTGGATCTA CGTCTGCGGG GGGATGTTCT TCTTCGTCAG CTTTGAGGGT
    AAAATCGTGA TGTACAAGAT CATCTACATG GTGCTCTTCC TCTTCTGTGT GGCCCTGTAT
    CAGGTGCACT ATGAATGGTG GAGGCGGATC CTGAAGTACT TCTGGATGTC AGTGGTTATC
    TACACGATGC TGGTGCTCAT CTTTATATAC ACATATCAGT TTGAAAACTT CCCAGGCTTG
    TGGCAAAACA TGACCGGACT GAAAAAAGAA AAGCTGGAGG ACCTTGGCCT GAAGCAGTTC
    ACGGTGGCCG AGCTGTTCAC GCGCATCTTC ATCCCTACCT CCTTCCTCCT GGTCTGCATC
    CTGCACCTGC ACTACTTCCA CGACCGGTTC CTGGAGCTCA CCGACCTGAA GTCCATCCCC
    AGCAAGGAGG ACAGCGCCAT CTATAGGCTG GCCCACCCCG AGGGAAGCCT TCCGGACCTT
    GCCATGATGA AGCTGACCGC TAGCCTGGAG GTGGCGGAGG TCAAGCAGCT GGCTGGGCCT
    GGGGAGGAGA TGCCGGGGGG ATGCCCTGCA AAAGCCAAGA AGGGCGAGCC TGGCAGGGAC
    AGCGAGGATG AGGATGAGGA TGAGGACGAA GAGGAGGAGG AGACGTCAGA TCTGAGGAAC
    AAGTGGCACT TGGTGATCGA CCGCCTCACC GTGCTGCTCC TGCGGTTCCT GGAGGGCTTC
    CACCAGCTGC AGACGTTCCT GTGGTGGATC CTGGAGCTGC ACATCGTCAA GATCGTGTCC
    TCCTACATCA TCTGGGTGTC CGTGAAGGAG GTGTCTCTGT TCAACTACGT GTTTCTTATT
    TCCTGGGCTT TTGCTCTGCC GTATGCCAGG CTCCGCCGTC TGGCCTCCAG TGTCTGCACC
    GTCTGGACAT GTGTGATCAT CGTCTGTAAG ATGCTGTACC AGCTGCAGAC CATCAAGCCT
    GAGAACTTCT CTGTGAACTG TTCGTTGCCA AATGAAAACC AGACGAACAT CCCCCTGCAC
    CAGCTGAACA AGTCTCTGCT CTACAGCGCA CCCATTGACC CCACAGAATG GGTGGGCCTC
    AGGAAGTCAT CTCCTCTACT TGTCTACCTG AGGAACAACC TGCTGATGCT GGCCATCCTG
    GCCTTCGAGG TCACCGTCTA CCGCCATCAG GAGTACTACC GAGGTCGGAA CAACCTCTCA
    ACCCCTGTGT CTAAAACCAT CTTCCACAAC ATCACAAGAC TCCAGCTGGA TGATGGACTT
    GTTAACTGTG CCAAGTATTT CATCAATTAC TTCTTCTACA AGTTTGGTCT GGAGACCTGT
    TTCCTCATGT CAGTTAACGT GATCGGCCAG CGCATGGATT TCTACGCCAT GATTCACGCC
    TGCTGGCTGA TTGCCGTCCT GTACCGGCGC AGGAGAAAGG CCATCGCCGA GATCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTCATCTG CATCGGCATC
    CCCCCTGCCC CCTGCCGAGA CTACCCCTGG AGGTTCAAGG GCGCCAGCTT CAATGACAAC
    ATCGTCAAGT GGCTGTACTT CCCTGACTTC ATCGTTCGGC CCAACCCTGT GTTCCTCGTG
    TATGATTTCA TGCTACTCCT GTGTGCCTCG CTACAACGGC AGATCTTTGA GGATGAGAAT
    AAGGCTGCCG TAAGGATCGT GGCAGGTGAC AACGTGGAGA TCTGCATGAA CCTGGACGCG
    GCCTCATTCA GCCAGCACAA TCCCGTGCCC GACTTCATCC ACTGCAGGTC GTATCTGGAC
    ATGTCCAAGG TGGTCATCTT CAGCTACCTC TTCTGGTTCG TGCTCACTAT CATCTTCATC
    ACTGGGACGA CCCGCATCAG CATCTTTTGC ATGGGCTACC TGGTGGCCTG CTTCTACTTC
    CTGCTCTTCG GGGGCGACTT GCTGCTGAAA CCCATCAAGA GCATCCTGCG CTACTGGGAC
    TGGCTGATCG CTTACAACGT CTTTGTGATC ACGATGAAAA ACATACTGTC GATAGGAGCA
    TGTGGCTATA TTGAAAAATT GGTTCAAAAT AGTTGCTGGT TGATCCAAGC ATTCAGCCTG
    GCCTGTACAG TCAAGGGCTA CAAAATGCCT GATGATGACT CCTCCTGTAA ACTGCCCAGT
    GGGGAGGCGG GAATCATTTG GGACAGCATA TGCTTTGCCT TCCTGCTGCT GCAGAGAAGA
    GTGTTCATGA GTTACTATTT CCTGCACGTG GTGGCAGATA TAAAAGCCTC GCAGATCCTG
    GCATCCAGAG GGGCTGAGCT TTTCCAGGCC ACCATTGTGA AGGCTGTGAA GGCCAGAATC
    GAGGAGGAGA AAAAGTCCAT GGACCAGCTG AAGAGGCAGA TGGACCGCAT CAAGGCCAGG
    CAACAGAAAT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCGGGGGAA
    GGCCAAGATC TCCAAAAAGT TTCTGAAGAG GATGACGAGA GAGAAGCAGA CAAACAGAAA
    ACCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ACTATTTGTT TGAAACGGAT AGTGAAGAGG AAGAAGAGGA AGAATTAAAA
    AAGGAAGACG AAGAGCCCCC CCGGAGGTCA GCTTTCCAGT TTGTTTATCA AGCCTGGATT
    ACTGACCCTA AAACAGCACT CCGACAGAGG CGCAAAGAGA AAAAAAGATC TGCAAGAGAA
    GAACAGAAAA GAAGGAGAAA AGAATCCGGG GAGGGTCCTG TGGAGTGGGA AGACCGGGAG
    GATGAGCCAG TCAAAAAGAA GTCTGATGGA CCAGACAACA TCATCAAGAG GATCTTTAAT
    ATTTTGAAGT TCACCTGGGT CCTGTTTCTG GCAACCGTGG ACAGTTTCAC AACGTGGCTC
    AACTCCATCT CCAGGGAGCA CCTCGACATC TCCACTGTCC TGAGGATCGA GCGCTGCATG
    CTGACCAGAG AGATCAAGAA GGGCAACGTC CCAACCCGGG AGAGCATCCA CATGTACTAC
    CAGAACCACA TCATGAACCT GTCCAGGGAG TCGGGACTGG ACACCATCGA CGAGCACGCT
    GGCACTGCTG CAGGTGCCCA GACTGCCCAC AGGATGGACA GCTTGGATTC CCACGACAGC
    ATCTCCAGCG AGCCCACGCA GTGCACGATG CTGTACTCGC GCCAGGGGAC CACGGAGACC
    ATCGAGGAAG TGGAGGCTGA GCAGGACGAG GAGGCGCCCG AGGGGGCCCG GGAGGCTGAG
    TCCCCACAGG ATCCCACGGA GGATGGTGAC GTGGAGGCCC CGCCATCCTA CAGCAAGGCT
    GTCAGCTTTG AGCGCCTGTC CTTCGACTCC CGGGACGACT CAGCCGGCCA GACCCACATG
    GAAGTCAGCC CGGATGACAG CCGCTCGGAT CGGCTGGAGG CCAGCATCCT GCCACCTCTG
    ACCCATGAGC TGACAGCCAG TGAGCTGCTG CTGAACAAGA TGTTCCATGA CGACGAGCTG
    GAGGAGTCAG AGAAGTTCTA CGTGGGGCAG CCTCGCTTCC TGCTGCTCTT CTACGCACTG
    TACAACACGC TGGTGGCCCG CTCTGAGATG GTGTGCTACT TTGTCATCAT CCTCAACCAC
    ATGGTCTCCG CCTCCATGAT TACCCTCGTG CTGCCCATCC TTATCTTCCT CTGGGCCATG
    CTGTCCGTCC CCCGGCCCAG CAGGCGCTTC TGGATGATGG CCATCGTCTA CACGGAGGTG
    GCCATTGTGG TCAAGTACTT CTTCCAATTT GGGTTCTTCC CCTGGAACAA GAATGTGGAA
    CTGAACAAGG ACAAGCCTTA CCACCCACCC AACATCATCG GGGTGGAGAA GAAGGACGCC
    TACGTGCTCT ACGACCTCAT CCAGCTGCTG GCGCTCTTCT TCCACCGCTC CATCCTAAAG
    TGTCACGGCC TCTGGGATGA AGACGACATC GTGGACAGCG GCTCAGACAA AGTGGACTCA
    GACGACGAGC TGTCCCTCGG CCATGGCAGG AGGGCCTCCT CCGACTCGCT CAAGTCCATC
    AACCTGGCCG CGTCCGTGGA GTCAGTGCAC GTGACCTTCC CCGAGCAGCC GGCTGCCGTC
    CGGAGGAAGC GCTCCAGCAG CGGCTCCCAG ACATCACAGG GGTCCAGCTT TTCCTCGAAT
    AGGTCCAAGA GAGGCAGCAC GAGCACCCGG AACAGCAGCC AGAAAGGAAG CAGCGTCGTG
    AGCGTCAAGC AGAAGAGCAG ACGGGAACTT TACATGGAGA AGTTGCAGGA CCACTTAATC
    AAAGCAAAAG CCTTTACCAT AAAGAAGACC CTGCAGCTCT ACGTGCCCAT CAGGCAGTTC
    TTCTACGACC TCATCCACCC CGACTACAGC GCCGTCACCG ATGTGTACGT GCTTATGTTC
    CTGGCGGACA CTGTGGACTT CGTCATCATT GTCTTCGGCT TCTGGGCCTT CGGGAAACAC
    TCGGCTGCGG CCGACATCAC GTCCTCGCTG TCCGAGGACC AGGTGCCGGG GCCCTTCCTG
    GTGATGGTGC TCATTCAGTT CGGGACCATG GTGGTGGACC GGGCGCTCTA CCTCAGGAAG
    ACTGTCCTAG GAAAGGTCAT CTTCCAGGTC ATCCTGGTGT TTGGGATTCA CTTCTGGATG
    TTCTTCATCC TGCCTGGCGT GACCGAGAGG AAGTTCAGCC AGAACCTGGT TGCGCAGCTG
    TGGTACTTCG TCAAGTGCGT GTACTTCGGG CTGTCCGCCT ACCAGATCCG CTGCGGCTAC
    CCAACTCGGG TCCTCGGCAA CTTCCTCACC AAGAGCTACA ACTACGTCAA CCTCTTCCTG
    TTCCAGGGGT TCCGCCTTGT CCCTTTCCTG ACGGAGCTGA GGGCTGTGAT GGACTGGGTG
    TGGACAGACA CGACCCTGAG CCTGTCCAGC TGGATCTGTG TGGAGGACAT CTATGCTCAC
    ATCTTCATCC TCAAGTGTTG GCGGGAGTCG GAAAAAAGGT ACCCGCAGCC CCGGGGGCAG
    AAGAAGAAGA AGGTGGTCAA GTACGGGATG GGGGGCATGA TCATCGTGCT GCTCATCTGC
    ATCGTCTGGT TCCCGCTACT CTTCATGTCT CTGGTCAAAT CCGTCGCTGG GGTTATCAAC
    CAGCCCCTGG ACGTCTCAGT CACAATCACC CTGGGCGGGT ACCAGCCTAT TTTCACAATG
    AGTGCCCAGC AAAGCCAGCT GAAAGTTATG GACCAGCCAA TGTTTAATAA ATTCATTAAA
    GCTTTTTCTA AGGACACTGG TGCTATGCAA TTTCTGGAAA ATTATGAAAA AGAAGACATA
    ACAGTCGCAG AACTGGAAGG AAACTCAAAT TCTTTATGGA CCATCAGCCC TCCCAGTAAG
    CAGAAAATGA TACACGAACT CAAGGACCCC AATAGTAGCT TCTCTGTTGT TTTTTCATGG
    AGTATTCAGA GAAACATGAG TCTGGGTGCA AAAGCAGAAA TAGCAACAGA TAAGCTTTCT
    TTCCCTCTTA AAAACGCGAC ACGAGAGAAT ATTGCTAAAA TGATAGCTGG CGGCGACACG
    GAAAGCTCCA GGACGCCCGT GACCATAGAA AGGATTTATC CATACTACGT GAAAGCACCC
    AGTGACTCTA ACTCCAAACC CATAAAGCAG CTTTTATCTG AAAATAATTT CATGAACATC
    ACCATCATTT TGTCCAGAGA CAATGCGACT AACTCGAACA GTGAGTGGTG GGTTCTCAAC
    TTGACTGGGA ACAGAATCTA CAACCAGGAA GCCCAGGCCC TGGAGCTGGT GGTCTTCAAC
    GACAAGGTCA GCCCCCCCAG CCTGGGGTTC CTGGCCGGCT ATGGTATCAT GGGCCTTTAC
    GCTTCCGTGG TGCTGGTGAT TGGGAAGTTT GTCCGGGAAT TCTTCAGCGG GATTTCTCAC
    TCCATCATGT TTGAAGAACT TCCCAACGTG GATCGGATTC TGAAATTGTG CACAGACATT
    TTTTTAGTCC GGGAGACCGG GGAGCTGGAG CTCGAGGAGG ATCTCTATGC CAAATTAATA
    TTCCTGTACC GGTCGCCAGA AACCATGATC AAATGGACCA GGGAGAAAAC AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32648
    Clone ID Related Accession (Same CDS sequence) XM_003587787.4 , XM_003587787.5 , XM_003583977.4
    Accession Version XM_003583977.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583977.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCTCGG AAGTGGTGTG CGGGCTCGTC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCCTGCG CCTTCCGGTA CAACGGGCTC TCCTTCGTCT ACCTCATCTA CCTCCTGCTC
    ATCCCCCTGT TCTCGGAACC AACTAAAGCT ACCATGCAAG GACACACGGG ACGGTTGTTA
    AAGTCTCTGT GCTTCACCAG TCTCTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG
    TTGGCGAGTC TGGAGGCACA GCACCGCATC GCACCTGGTT ACAACTGCTC AACCTGGGAG
    AAGACTTTCC GACAAATCGG CTTCGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC
    CGAGTGTTTG TGCCTGACGT GGGCATGTTC ATCGCCAGCC TGACCATCTG GCTGGTCTGT
    AGGAACATTG TTCAGAAGCC AGTGGCCGAA GAGGCCGCGC AGTGTAACCC GGAATTTGAG
    AACGAAGAAT CGGCTGGAGG AGAAAAAATA GACTCCGAAG AGGCCCTGAT CTATGAAGAG
    GACTTAGATG AAGGAGACGG TGCTGAAGGC GATTTGGAGG AAAGCGCGAA ATTAAAACTC
    TTCCGCAGGC TGGCCTCTGT GGCCTCCAAG CTGAAGGAGT TTATTGGCAA CATGATAACC
    ACTGCGGGGA AGGTTGTTGT GACGATTTTA CTGGGTTCAT CAGGGATGAT GCTGCCCTCT
    CTGACCTCGT CCGTGTACTT CTTCGTGTTT CTGGGCCTGT GCACTTGGTG GTCCTGGTGC
    CGGACCTTTG ACCCCTTGCT GTTCAGCTGT CTCTGCGTCC TGCTGGCCAT CTTCACCGCG
    GGACACCTGA TCGGACTCTA TCTGTACCAG TTCCAGTTCT TCCAGGAAGC CGTTCCCCCC
    AACGACTACT ATGCCAGGTT GTTTGGTATC AAATCAGTCA TTCAGACAGA CTGTTCCAGC
    ACATGGAAGA TCATTGCCAA CCCCGACCTG TCCTGGTACC ACCATGCCAA CCCCATTGTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACC CTGATCCGAA TCTGGCTGCA GGAGCCCGTT
    GTGCAGGAAG AGAGGACCAA GGAGGAGGAC AGAGCCCTGG CCTGCAGCCC TGCGCCCATC
    ACGGCCGAGA GGAGGCGGAG CCTGTGGTAT GCGACCCACT ACCCCACGGA CGAGAGGAAA
    CTTTTATCCA TGACTCAGGA TGACTACAAG CCGTCCGACG GCCTGCTGGT GACCGTGAAC
    GGCAACCCCG TGGACTACCA CACTATCCAC CCCAGCCTCC CGCTGGAGAA CGGCCCCGCC
    AAGGCTGACC TGTACTCCAC GCCGCAGTAC CGCTGGGAGC CTGCCGCTGC CTCCCCCGAA
    AAGAAGGAGG AAGACGATGA TGAGAAAGAA GAGTTTGAGG AGGAGCGGAG CCGGGAGGAG
    AAGAGGAGTG TCAAAGTGCA CGCCATGGTG TCTGTGTTCC AGTTCATCAT GAAGCAGAGC
    TACATCTGTG CCCTTATCGC CATGATGGCC TGGAGCATCA CCTACCACAG CTGGCTGACC
    TTTGTGCTGC TCATCTGGTC CTGCACTCTG TGGATGATCC GCAACCGGAG GAAGTATGCT
    ATGATCAGCT CCCCCTTCAT GGTTGTCTAT GGAAACCTGC TGCTCATACT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA AATTAAAAAG GTCCCAGGTT TCTTAGAAAA GAAGGAGCCG
    GGGGAGCTCG CCTCAAAGAT TCTGTTCACC ATCACATTCT GGCTGCTGCT GAGACAGCAC
    CTCACAGAGC AGAAAGCTCT CCAAGAAAAG GAAGCTCTTC TGTCTGAGGT CAAAATCGGA
    AGTCAGGAAA GAGAAGAAAA AGATGATGAA CAGGACTTAC AGGTGGACAG TGAGGCTCAG
    GAGAAGGAGG AAGAAGAGGA GCCGAAGGAG GAGCAGCAGG AGCGGAGAGA GGAAGAGGAA
    GAGGAAGAAG CAGATGAGCA GGACGTGATG AAGGTGCTGG GGAACCTGGT GGTGGCCCTG
    TTCATCAAGT ACTGGATCTA CGTCTGCGGG GGGATGTTCT TCTTCGTCAG CTTTGAGGGT
    AAAATCGTGA TGTACAAGAT CATCTACATG GTGCTCTTCC TCTTCTGTGT GGCCCTGTAT
    CAGGTGCACT ATGAATGGTG GAGGCGGATC CTGAAGTACT TCTGGATGTC AGTGGTTATC
    TACACGATGC TGGTGCTCAT CTTTATATAC ACATATCAGT TTGAAAACTT CCCAGGCTTG
    TGGCAAAACA TGACCGGACT GAAAAAAGAA AAGCTGGAGG ACCTTGGCCT GAAGCAGTTC
    ACGGTGGCCG AGCTGTTCAC GCGCATCTTC ATCCCTACCT CCTTCCTCCT GGTCTGCATC
    CTGCACCTGC ACTACTTCCA CGACCGGTTC CTGGAGCTCA CCGACCTGAA GTCCATCCCC
    AGCAAGGAGG ACAGCGCCAT CTATAGGCTG GCCCACCCCG AGGGAAGCCT TCCGGACCTT
    GCCATGATGA AGCTGACCGC TAGCCTGGAG GTGGCGGAGG TCAAGCAGCT GGCTGGGCCT
    GGGGAGGAGA TGCCGGGGGG ATGCCCTGCA AAAGCCAAGA AGGGCGAGCC TGGCAGGGAC
    AGCGAGGATG AGGATGAGGA TGAGGACGAA GAGGAGGAGG AGACGTCAGA TCTGAGGAAC
    AAGTGGCACT TGGTGATCGA CCGCCTCACC GTGCTGCTCC TGCGGTTCCT GGAGGGCTTC
    CACCAGCTGC AGACGTTCCT GTGGTGGATC CTGGAGCTGC ACATCGTCAA GATCGTGTCC
    TCCTACATCA TCTGGGTGTC CGTGAAGGAG GTGTCTCTGT TCAACTACGT GTTTCTTATT
    TCCTGGGCTT TTGCTCTGCC GTATGCCAGG CTCCGCCGTC TGGCCTCCAG TGTCTGCACC
    GTCTGGACAT GTGTGATCAT CGTCTGTAAG ATGCTGTACC AGCTGCAGAC CATCAAGCCT
    GAGAACTTCT CTGTGAACTG TTCGTTGCCA AATGAAAACC AGACGAACAT CCCCCTGCAC
    CAGCTGAACA AGTCTCTGCT CTACAGCGCA CCCATTGACC CCACAGAATG GGTGGGCCTC
    AGGAAGTCAT CTCCTCTACT TGTCTACCTG AGGAACAACC TGCTGATGCT GGCCATCCTG
    GCCTTCGAGG TCACCGTCTA CCGCCATCAG GAGTACTACC GAGGTCGGAA CAACCTCTCA
    ACCCCTGTGT CTAAAACCAT CTTCCACAAC ATCACAAGAC TCCAGCTGGA TGATGGACTT
    GTTAACTGTG CCAAGTATTT CATCAATTAC TTCTTCTACA AGTTTGGTCT GGAGACCTGT
    TTCCTCATGT CAGTTAACGT GATCGGCCAG CGCATGGATT TCTACGCCAT GATTCACGCC
    TGCTGGCTGA TTGCCGTCCT GTACCGGCGC AGGAGAAAGG CCATCGCCGA GATCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTCATCTG CATCGGCATC
    CCCCCTGCCC CCTGCCGAGA CTACCCCTGG AGGTTCAAGG GCGCCAGCTT CAATGACAAC
    ATCGTCAAGT GGCTGTACTT CCCTGACTTC ATCGTTCGGC CCAACCCTGT GTTCCTCGTG
    TATGATTTCA TGCTACTCCT GTGTGCCTCG CTACAACGGC AGATCTTTGA GGATGAGAAT
    AAGGCTGCCG TAAGGATCGT GGCAGGTGAC AACGTGGAGA TCTGCATGAA CCTGGACGCG
    GCCTCATTCA GCCAGCACAA TCCCGTGCCC GACTTCATCC ACTGCAGGTC GTATCTGGAC
    ATGTCCAAGG TGGTCATCTT CAGCTACCTC TTCTGGTTCG TGCTCACTAT CATCTTCATC
    ACTGGGACGA CCCGCATCAG CATCTTTTGC ATGGGCTACC TGGTGGCCTG CTTCTACTTC
    CTGCTCTTCG GGGGCGACTT GCTGCTGAAA CCCATCAAGA GCATCCTGCG CTACTGGGAC
    TGGCTGATCG CTTACAACGT CTTTGTGATC ACGATGAAAA ACATACTGTC GATAGGAGCA
    TGTGGCTATA TTGAAAAATT GGTTCAAAAT AGTTGCTGGT TGATCCAAGC ATTCAGCCTG
    GCCTGTACAG TCAAGGGCTA CAAAATGCCT GATGATGACT CCTCCTGTAA ACTGCCCAGT
    GGGGAGGCGG GAATCATTTG GGACAGCATA TGCTTTGCCT TCCTGCTGCT GCAGAGAAGA
    GTGTTCATGA GTTACTATTT CCTGCACGTG GTGGCAGATA TAAAAGCCTC GCAGATCCTG
    GCATCCAGAG GGGCTGAGCT TTTCCAGGCC ACCATTGTGA AGGCTGTGAA GGCCAGAATC
    GAGGAGGAGA AAAAGTCCAT GGACCAGCTG AAGAGGCAGA TGGACCGCAT CAAGGCCAGG
    CAACAGAAAT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCGGGGGAA
    GGCCAAGATC TCCAAAAAGT TTCTGAAGAG GATGACGAGA GAGAAGCAGA CAAACAGAAA
    ACCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ACTATTTGTT TGAAACGGAT AGTGAAGAGG AAGAAGAGGA AGAATTAAAA
    AAGGAAGACG AAGAGCCCCC CCGGAGGTCA GCTTTCCAGT TTGTTTATCA AGCCTGGATT
    ACTGACCCTA AAACAGCACT CCGACAGAGG CGCAAAGAGA AAAAAAGATC TGCAAGAGAA
    GAACAGAAAA GAAGGAGAAA AGAATCCGGG GAGGGTCCTG TGGAGTGGGA AGACCGGGAG
    GATGAGCCAG TCAAAAAGAA GTCTGATGGA CCAGACAACA TCATCAAGAG GATCTTTAAT
    ATTTTGAAGT TCACCTGGGT CCTGTTTCTG GCAACCGTGG ACAGTTTCAC AACGTGGCTC
    AACTCCATCT CCAGGGAGCA CCTCGACATC TCCACTGTCC TGAGGATCGA GCGCTGCATG
    CTGACCAGAG AGATCAAGAA GGGCAACGTC CCAACCCGGG AGAGCATCCA CATGTACTAC
    CAGAACCACA TCATGAACCT GTCCAGGGAG TCGGGACTGG ACACCATCGA CGAGCACGCT
    GGCACTGCTG CAGGTGCCCA GACTGCCCAC AGGATGGACA GCTTGGATTC CCACGACAGC
    ATCTCCAGCG AGCCCACGCA GTGCACGATG CTGTACTCGC GCCAGGGGAC CACGGAGACC
    ATCGAGGAAG TGGAGGCTGA GCAGGACGAG GAGGCGCCCG AGGGGGCCCG GGAGGCTGAG
    TCCCCACAGG ATCCCACGGA GGATGGTGAC GTGGAGGCCC CGCCATCCTA CAGCAAGGCT
    GTCAGCTTTG AGCGCCTGTC CTTCGACTCC CGGGACGACT CAGCCGGCCA GACCCACATG
    GAAGTCAGCC CGGATGACAG CCGCTCGGAT CGGCTGGAGG CCAGCATCCT GCCACCTCTG
    ACCCATGAGC TGACAGCCAG TGAGCTGCTG CTGAACAAGA TGTTCCATGA CGACGAGCTG
    GAGGAGTCAG AGAAGTTCTA CGTGGGGCAG CCTCGCTTCC TGCTGCTCTT CTACGCACTG
    TACAACACGC TGGTGGCCCG CTCTGAGATG GTGTGCTACT TTGTCATCAT CCTCAACCAC
    ATGGTCTCCG CCTCCATGAT TACCCTCGTG CTGCCCATCC TTATCTTCCT CTGGGCCATG
    CTGTCCGTCC CCCGGCCCAG CAGGCGCTTC TGGATGATGG CCATCGTCTA CACGGAGGTG
    GCCATTGTGG TCAAGTACTT CTTCCAATTT GGGTTCTTCC CCTGGAACAA GAATGTGGAA
    CTGAACAAGG ACAAGCCTTA CCACCCACCC AACATCATCG GGGTGGAGAA GAAGGACGCC
    TACGTGCTCT ACGACCTCAT CCAGCTGCTG GCGCTCTTCT TCCACCGCTC CATCCTAAAG
    TGTCACGGCC TCTGGGATGA AGACGACATC GTGGACAGCG GCTCAGACAA AGTGGACTCA
    GACGACGAGC TGTCCCTCGG CCATGGCAGG AGGGCCTCCT CCGACTCGCT CAAGTCCATC
    AACCTGGCCG CGTCCGTGGA GTCAGTGCAC GTGACCTTCC CCGAGCAGCC GGCTGCCGTC
    CGGAGGAAGC GCTCCAGCAG CGGCTCCCAG ACATCACAGG GGTCCAGCTT TTCCTCGAAT
    AGGTCCAAGA GAGGCAGCAC GAGCACCCGG AACAGCAGCC AGAAAGGAAG CAGCGTCGTG
    AGCGTCAAGC AGAAGAGCAG ACGGGAACTT TACATGGAGA AGTTGCAGGA CCACTTAATC
    AAAGCAAAAG CCTTTACCAT AAAGAAGACC CTGCAGCTCT ACGTGCCCAT CAGGCAGTTC
    TTCTACGACC TCATCCACCC CGACTACAGC GCCGTCACCG ATGTGTACGT GCTTATGTTC
    CTGGCGGACA CTGTGGACTT CGTCATCATT GTCTTCGGCT TCTGGGCCTT CGGGAAACAC
    TCGGCTGCGG CCGACATCAC GTCCTCGCTG TCCGAGGACC AGGTGCCGGG GCCCTTCCTG
    GTGATGGTGC TCATTCAGTT CGGGACCATG GTGGTGGACC GGGCGCTCTA CCTCAGGAAG
    ACTGTCCTAG GAAAGGTCAT CTTCCAGGTC ATCCTGGTGT TTGGGATTCA CTTCTGGATG
    TTCTTCATCC TGCCTGGCGT GACCGAGAGG AAGTTCAGCC AGAACCTGGT TGCGCAGCTG
    TGGTACTTCG TCAAGTGCGT GTACTTCGGG CTGTCCGCCT ACCAGATCCG CTGCGGCTAC
    CCAACTCGGG TCCTCGGCAA CTTCCTCACC AAGAGCTACA ACTACGTCAA CCTCTTCCTG
    TTCCAGGGGT TCCGCCTTGT CCCTTTCCTG ACGGAGCTGA GGGCTGTGAT GGACTGGGTG
    TGGACAGACA CGACCCTGAG CCTGTCCAGC TGGATCTGTG TGGAGGACAT CTATGCTCAC
    ATCTTCATCC TCAAGTGTTG GCGGGAGTCG GAAAAAAGGT ACCCGCAGCC CCGGGGGCAG
    AAGAAGAAGA AGGTGGTCAA GTACGGGATG GGGGGCATGA TCATCGTGCT GCTCATCTGC
    ATCGTCTGGT TCCCGCTACT CTTCATGTCT CTGGTCAAAT CCGTCGCTGG GGTTATCAAC
    CAGCCCCTGG ACGTCTCAGT CACAATCACC CTGGGCGGGT ACCAGCCTAT TTTCACAATG
    AGTGCCCAGC AAAGCCAGCT GAAAGTTATG GACCAGCCAA TGTTTAATAA ATTCATTAAA
    GCTTTTTCTA AGGACACTGG TGCTATGCAA TTTCTGGAAA ATTATGAAAA AGAAGACATA
    ACAGTCGCAG AACTGGAAGG AAACTCAAAT TCTTTATGGA CCATCAGCCC TCCCAGTAAG
    CAGAAAATGA TACACGAACT CAAGGACCCC AATAGTAGCT TCTCTGTTGT TTTTTCATGG
    AGTATTCAGA GAAACATGAG TCTGGGTGCA AAAGCAGAAA TAGCAACAGA TAAGCTTTCT
    TTCCCTCTTA AAAACGCGAC ACGAGAGAAT ATTGCTAAAA TGATAGCTGG CGGCGACACG
    GAAAGCTCCA GGACGCCCGT GACCATAGAA AGGATTTATC CATACTACGT GAAAGCACCC
    AGTGACTCTA ACTCCAAACC CATAAAGCAG CTTTTATCTG AAAATAATTT CATGAACATC
    ACCATCATTT TGTCCAGAGA CAATGCGACT AACTCGAACA GTGAGTGGTG GGTTCTCAAC
    TTGACTGGGA ACAGAATCTA CAACCAGGAA GCCCAGGCCC TGGAGCTGGT GGTCTTCAAC
    GACAAGGTCA GCCCCCCCAG CCTGGGGTTC CTGGCCGGCT ATGGTATCAT GGGCCTTTAC
    GCTTCCGTGG TGCTGGTGAT TGGGAAGTTT GTCCGGGAAT TCTTCAGCGG GATTTCTCAC
    TCCATCATGT TTGAAGAACT TCCCAACGTG GATCGGATTC TGAAATTGTG CACAGACATT
    TTTTTAGTCC GGGAGACCGG GGAGCTGGAG CTCGAGGAGG ATCTCTATGC CAAATTAATA
    TTCCTGTACC GGTCGCCAGA AACCATGATC AAATGGACCA GGGAGAAAAC AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003584025.3
    CDS27..8183
    Translation

    Target ORF information:

    RefSeq Version XM_003583977.4
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003583977.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCTCGG AAGTGGTGTG CGGGCTCGTC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
    GTAGCCTGCG CCTTCCGGTA CAACGGGCTC TCCTTCGTCT ACCTCATCTA CCTCCTGCTC
    ATCCCCCTGT TCTCGGAACC AACTAAAGCT ACCATGCAAG GACACACGGG ACGGTTGTTA
    AAGTCTCTGT GCTTCACCAG TCTCTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG
    TTGGCGAGTC TGGAGGCACA GCACCGCATC GCACCTGGTT ACAACTGCTC AACCTGGGAG
    AAGACTTTCC GACAAATCGG CTTCGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC
    CGAGTGTTTG TGCCTGACGT GGGCATGTTC ATCGCCAGCC TGACCATCTG GCTGGTCTGT
    AGGAACATTG TTCAGAAGCC AGTGGCCGAA GAGGCCGCGC AGTGTAACCC GGAATTTGAG
    AACGAAGAAT CGGCTGGAGG AGAAAAAATA GACTCCGAAG AGGCCCTGAT CTATGAAGAG
    GACTTAGATG AAGGAGACGG TGCTGAAGGC GATTTGGAGG AAAGCGCGAA ATTAAAACTC
    TTCCGCAGGC TGGCCTCTGT GGCCTCCAAG CTGAAGGAGT TTATTGGCAA CATGATAACC
    ACTGCGGGGA AGGTTGTTGT GACGATTTTA CTGGGTTCAT CAGGGATGAT GCTGCCCTCT
    CTGACCTCGT CCGTGTACTT CTTCGTGTTT CTGGGCCTGT GCACTTGGTG GTCCTGGTGC
    CGGACCTTTG ACCCCTTGCT GTTCAGCTGT CTCTGCGTCC TGCTGGCCAT CTTCACCGCG
    GGACACCTGA TCGGACTCTA TCTGTACCAG TTCCAGTTCT TCCAGGAAGC CGTTCCCCCC
    AACGACTACT ATGCCAGGTT GTTTGGTATC AAATCAGTCA TTCAGACAGA CTGTTCCAGC
    ACATGGAAGA TCATTGCCAA CCCCGACCTG TCCTGGTACC ACCATGCCAA CCCCATTGTC
    CTGCTGGTGA TGTACTACAC GCTGGCCACC CTGATCCGAA TCTGGCTGCA GGAGCCCGTT
    GTGCAGGAAG AGAGGACCAA GGAGGAGGAC AGAGCCCTGG CCTGCAGCCC TGCGCCCATC
    ACGGCCGAGA GGAGGCGGAG CCTGTGGTAT GCGACCCACT ACCCCACGGA CGAGAGGAAA
    CTTTTATCCA TGACTCAGGA TGACTACAAG CCGTCCGACG GCCTGCTGGT GACCGTGAAC
    GGCAACCCCG TGGACTACCA CACTATCCAC CCCAGCCTCC CGCTGGAGAA CGGCCCCGCC
    AAGGCTGACC TGTACTCCAC GCCGCAGTAC CGCTGGGAGC CTGCCGCTGC CTCCCCCGAA
    AAGAAGGAGG AAGACGATGA TGAGAAAGAA GAGTTTGAGG AGGAGCGGAG CCGGGAGGAG
    AAGAGGAGTG TCAAAGTGCA CGCCATGGTG TCTGTGTTCC AGTTCATCAT GAAGCAGAGC
    TACATCTGTG CCCTTATCGC CATGATGGCC TGGAGCATCA CCTACCACAG CTGGCTGACC
    TTTGTGCTGC TCATCTGGTC CTGCACTCTG TGGATGATCC GCAACCGGAG GAAGTATGCT
    ATGATCAGCT CCCCCTTCAT GGTTGTCTAT GGAAACCTGC TGCTCATACT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA AATTAAAAAG GTCCCAGGTT TCTTAGAAAA GAAGGAGCCG
    GGGGAGCTCG CCTCAAAGAT TCTGTTCACC ATCACATTCT GGCTGCTGCT GAGACAGCAC
    CTCACAGAGC AGAAAGCTCT CCAAGAAAAG GAAGCTCTTC TGTCTGAGGT CAAAATCGGA
    AGTCAGGAAA GAGAAGAAAA AGATGATGAA CAGGACTTAC AGGTGGACAG TGAGGCTCAG
    GAGAAGGAGG AAGAAGAGGA GCCGAAGGAG GAGCAGCAGG AGCGGAGAGA GGAAGAGGAA
    GAGGAAGAAG CAGATGAGCA GGACGTGATG AAGGTGCTGG GGAACCTGGT GGTGGCCCTG
    TTCATCAAGT ACTGGATCTA CGTCTGCGGG GGGATGTTCT TCTTCGTCAG CTTTGAGGGT
    AAAATCGTGA TGTACAAGAT CATCTACATG GTGCTCTTCC TCTTCTGTGT GGCCCTGTAT
    CAGGTGCACT ATGAATGGTG GAGGCGGATC CTGAAGTACT TCTGGATGTC AGTGGTTATC
    TACACGATGC TGGTGCTCAT CTTTATATAC ACATATCAGT TTGAAAACTT CCCAGGCTTG
    TGGCAAAACA TGACCGGACT GAAAAAAGAA AAGCTGGAGG ACCTTGGCCT GAAGCAGTTC
    ACGGTGGCCG AGCTGTTCAC GCGCATCTTC ATCCCTACCT CCTTCCTCCT GGTCTGCATC
    CTGCACCTGC ACTACTTCCA CGACCGGTTC CTGGAGCTCA CCGACCTGAA GTCCATCCCC
    AGCAAGGAGG ACAGCGCCAT CTATAGGCTG GCCCACCCCG AGGGAAGCCT TCCGGACCTT
    GCCATGATGA AGCTGACCGC TAGCCTGGAG GTGGCGGAGG TCAAGCAGCT GGCTGGGCCT
    GGGGAGGAGA TGCCGGGGGG ATGCCCTGCA AAAGCCAAGA AGGGCGAGCC TGGCAGGGAC
    AGCGAGGATG AGGATGAGGA TGAGGACGAA GAGGAGGAGG AGACGTCAGA TCTGAGGAAC
    AAGTGGCACT TGGTGATCGA CCGCCTCACC GTGCTGCTCC TGCGGTTCCT GGAGGGCTTC
    CACCAGCTGC AGACGTTCCT GTGGTGGATC CTGGAGCTGC ACATCGTCAA GATCGTGTCC
    TCCTACATCA TCTGGGTGTC CGTGAAGGAG GTGTCTCTGT TCAACTACGT GTTTCTTATT
    TCCTGGGCTT TTGCTCTGCC GTATGCCAGG CTCCGCCGTC TGGCCTCCAG TGTCTGCACC
    GTCTGGACAT GTGTGATCAT CGTCTGTAAG ATGCTGTACC AGCTGCAGAC CATCAAGCCT
    GAGAACTTCT CTGTGAACTG TTCGTTGCCA AATGAAAACC AGACGAACAT CCCCCTGCAC
    CAGCTGAACA AGTCTCTGCT CTACAGCGCA CCCATTGACC CCACAGAATG GGTGGGCCTC
    AGGAAGTCAT CTCCTCTACT TGTCTACCTG AGGAACAACC TGCTGATGCT GGCCATCCTG
    GCCTTCGAGG TCACCGTCTA CCGCCATCAG GAGTACTACC GAGGTCGGAA CAACCTCTCA
    ACCCCTGTGT CTAAAACCAT CTTCCACAAC ATCACAAGAC TCCAGCTGGA TGATGGACTT
    GTTAACTGTG CCAAGTATTT CATCAATTAC TTCTTCTACA AGTTTGGTCT GGAGACCTGT
    TTCCTCATGT CAGTTAACGT GATCGGCCAG CGCATGGATT TCTACGCCAT GATTCACGCC
    TGCTGGCTGA TTGCCGTCCT GTACCGGCGC AGGAGAAAGG CCATCGCCGA GATCTGGCCC
    AAGTACTGCT GCTTCCTGGC TTGCATCATC ACCTTCCAGT ACTTCATCTG CATCGGCATC
    CCCCCTGCCC CCTGCCGAGA CTACCCCTGG AGGTTCAAGG GCGCCAGCTT CAATGACAAC
    ATCGTCAAGT GGCTGTACTT CCCTGACTTC ATCGTTCGGC CCAACCCTGT GTTCCTCGTG
    TATGATTTCA TGCTACTCCT GTGTGCCTCG CTACAACGGC AGATCTTTGA GGATGAGAAT
    AAGGCTGCCG TAAGGATCGT GGCAGGTGAC AACGTGGAGA TCTGCATGAA CCTGGACGCG
    GCCTCATTCA GCCAGCACAA TCCCGTGCCC GACTTCATCC ACTGCAGGTC GTATCTGGAC
    ATGTCCAAGG TGGTCATCTT CAGCTACCTC TTCTGGTTCG TGCTCACTAT CATCTTCATC
    ACTGGGACGA CCCGCATCAG CATCTTTTGC ATGGGCTACC TGGTGGCCTG CTTCTACTTC
    CTGCTCTTCG GGGGCGACTT GCTGCTGAAA CCCATCAAGA GCATCCTGCG CTACTGGGAC
    TGGCTGATCG CTTACAACGT CTTTGTGATC ACGATGAAAA ACATACTGTC GATAGGAGCA
    TGTGGCTATA TTGAAAAATT GGTTCAAAAT AGTTGCTGGT TGATCCAAGC ATTCAGCCTG
    GCCTGTACAG TCAAGGGCTA CAAAATGCCT GATGATGACT CCTCCTGTAA ACTGCCCAGT
    GGGGAGGCGG GAATCATTTG GGACAGCATA TGCTTTGCCT TCCTGCTGCT GCAGAGAAGA
    GTGTTCATGA GTTACTATTT CCTGCACGTG GTGGCAGATA TAAAAGCCTC GCAGATCCTG
    GCATCCAGAG GGGCTGAGCT TTTCCAGGCC ACCATTGTGA AGGCTGTGAA GGCCAGAATC
    GAGGAGGAGA AAAAGTCCAT GGACCAGCTG AAGAGGCAGA TGGACCGCAT CAAGGCCAGG
    CAACAGAAAT ACAAAAAGGG TAAGGAGAGG ATGCTGAGCT TGACCCAGGA GTCGGGGGAA
    GGCCAAGATC TCCAAAAAGT TTCTGAAGAG GATGACGAGA GAGAAGCAGA CAAACAGAAA
    ACCAAGGGCA AAAAAAAGCA GTGGTGGCGG CCTTGGGTTG ATCATGCTTC CATGGTCAGG
    AGTGGAGATT ACTATTTGTT TGAAACGGAT AGTGAAGAGG AAGAAGAGGA AGAATTAAAA
    AAGGAAGACG AAGAGCCCCC CCGGAGGTCA GCTTTCCAGT TTGTTTATCA AGCCTGGATT
    ACTGACCCTA AAACAGCACT CCGACAGAGG CGCAAAGAGA AAAAAAGATC TGCAAGAGAA
    GAACAGAAAA GAAGGAGAAA AGAATCCGGG GAGGGTCCTG TGGAGTGGGA AGACCGGGAG
    GATGAGCCAG TCAAAAAGAA GTCTGATGGA CCAGACAACA TCATCAAGAG GATCTTTAAT
    ATTTTGAAGT TCACCTGGGT CCTGTTTCTG GCAACCGTGG ACAGTTTCAC AACGTGGCTC
    AACTCCATCT CCAGGGAGCA CCTCGACATC TCCACTGTCC TGAGGATCGA GCGCTGCATG
    CTGACCAGAG AGATCAAGAA GGGCAACGTC CCAACCCGGG AGAGCATCCA CATGTACTAC
    CAGAACCACA TCATGAACCT GTCCAGGGAG TCGGGACTGG ACACCATCGA CGAGCACGCT
    GGCACTGCTG CAGGTGCCCA GACTGCCCAC AGGATGGACA GCTTGGATTC CCACGACAGC
    ATCTCCAGCG AGCCCACGCA GTGCACGATG CTGTACTCGC GCCAGGGGAC CACGGAGACC
    ATCGAGGAAG TGGAGGCTGA GCAGGACGAG GAGGCGCCCG AGGGGGCCCG GGAGGCTGAG
    TCCCCACAGG ATCCCACGGA GGATGGTGAC GTGGAGGCCC CGCCATCCTA CAGCAAGGCT
    GTCAGCTTTG AGCGCCTGTC CTTCGACTCC CGGGACGACT CAGCCGGCCA GACCCACATG
    GAAGTCAGCC CGGATGACAG CCGCTCGGAT CGGCTGGAGG CCAGCATCCT GCCACCTCTG
    ACCCATGAGC TGACAGCCAG TGAGCTGCTG CTGAACAAGA TGTTCCATGA CGACGAGCTG
    GAGGAGTCAG AGAAGTTCTA CGTGGGGCAG CCTCGCTTCC TGCTGCTCTT CTACGCACTG
    TACAACACGC TGGTGGCCCG CTCTGAGATG GTGTGCTACT TTGTCATCAT CCTCAACCAC
    ATGGTCTCCG CCTCCATGAT TACCCTCGTG CTGCCCATCC TTATCTTCCT CTGGGCCATG
    CTGTCCGTCC CCCGGCCCAG CAGGCGCTTC TGGATGATGG CCATCGTCTA CACGGAGGTG
    GCCATTGTGG TCAAGTACTT CTTCCAATTT GGGTTCTTCC CCTGGAACAA GAATGTGGAA
    CTGAACAAGG ACAAGCCTTA CCACCCACCC AACATCATCG GGGTGGAGAA GAAGGACGCC
    TACGTGCTCT ACGACCTCAT CCAGCTGCTG GCGCTCTTCT TCCACCGCTC CATCCTAAAG
    TGTCACGGCC TCTGGGATGA AGACGACATC GTGGACAGCG GCTCAGACAA AGTGGACTCA
    GACGACGAGC TGTCCCTCGG CCATGGCAGG AGGGCCTCCT CCGACTCGCT CAAGTCCATC
    AACCTGGCCG CGTCCGTGGA GTCAGTGCAC GTGACCTTCC CCGAGCAGCC GGCTGCCGTC
    CGGAGGAAGC GCTCCAGCAG CGGCTCCCAG ACATCACAGG GGTCCAGCTT TTCCTCGAAT
    AGGTCCAAGA GAGGCAGCAC GAGCACCCGG AACAGCAGCC AGAAAGGAAG CAGCGTCGTG
    AGCGTCAAGC AGAAGAGCAG ACGGGAACTT TACATGGAGA AGTTGCAGGA CCACTTAATC
    AAAGCAAAAG CCTTTACCAT AAAGAAGACC CTGCAGCTCT ACGTGCCCAT CAGGCAGTTC
    TTCTACGACC TCATCCACCC CGACTACAGC GCCGTCACCG ATGTGTACGT GCTTATGTTC
    CTGGCGGACA CTGTGGACTT CGTCATCATT GTCTTCGGCT TCTGGGCCTT CGGGAAACAC
    TCGGCTGCGG CCGACATCAC GTCCTCGCTG TCCGAGGACC AGGTGCCGGG GCCCTTCCTG
    GTGATGGTGC TCATTCAGTT CGGGACCATG GTGGTGGACC GGGCGCTCTA CCTCAGGAAG
    ACTGTCCTAG GAAAGGTCAT CTTCCAGGTC ATCCTGGTGT TTGGGATTCA CTTCTGGATG
    TTCTTCATCC TGCCTGGCGT GACCGAGAGG AAGTTCAGCC AGAACCTGGT TGCGCAGCTG
    TGGTACTTCG TCAAGTGCGT GTACTTCGGG CTGTCCGCCT ACCAGATCCG CTGCGGCTAC
    CCAACTCGGG TCCTCGGCAA CTTCCTCACC AAGAGCTACA ACTACGTCAA CCTCTTCCTG
    TTCCAGGGGT TCCGCCTTGT CCCTTTCCTG ACGGAGCTGA GGGCTGTGAT GGACTGGGTG
    TGGACAGACA CGACCCTGAG CCTGTCCAGC TGGATCTGTG TGGAGGACAT CTATGCTCAC
    ATCTTCATCC TCAAGTGTTG GCGGGAGTCG GAAAAAAGGT ACCCGCAGCC CCGGGGGCAG
    AAGAAGAAGA AGGTGGTCAA GTACGGGATG GGGGGCATGA TCATCGTGCT GCTCATCTGC
    ATCGTCTGGT TCCCGCTACT CTTCATGTCT CTGGTCAAAT CCGTCGCTGG GGTTATCAAC
    CAGCCCCTGG ACGTCTCAGT CACAATCACC CTGGGCGGGT ACCAGCCTAT TTTCACAATG
    AGTGCCCAGC AAAGCCAGCT GAAAGTTATG GACCAGCCAA TGTTTAATAA ATTCATTAAA
    GCTTTTTCTA AGGACACTGG TGCTATGCAA TTTCTGGAAA ATTATGAAAA AGAAGACATA
    ACAGTCGCAG AACTGGAAGG AAACTCAAAT TCTTTATGGA CCATCAGCCC TCCCAGTAAG
    CAGAAAATGA TACACGAACT CAAGGACCCC AATAGTAGCT TCTCTGTTGT TTTTTCATGG
    AGTATTCAGA GAAACATGAG TCTGGGTGCA AAAGCAGAAA TAGCAACAGA TAAGCTTTCT
    TTCCCTCTTA AAAACGCGAC ACGAGAGAAT ATTGCTAAAA TGATAGCTGG CGGCGACACG
    GAAAGCTCCA GGACGCCCGT GACCATAGAA AGGATTTATC CATACTACGT GAAAGCACCC
    AGTGACTCTA ACTCCAAACC CATAAAGCAG CTTTTATCTG AAAATAATTT CATGAACATC
    ACCATCATTT TGTCCAGAGA CAATGCGACT AACTCGAACA GTGAGTGGTG GGTTCTCAAC
    TTGACTGGGA ACAGAATCTA CAACCAGGAA GCCCAGGCCC TGGAGCTGGT GGTCTTCAAC
    GACAAGGTCA GCCCCCCCAG CCTGGGGTTC CTGGCCGGCT ATGGTATCAT GGGCCTTTAC
    GCTTCCGTGG TGCTGGTGAT TGGGAAGTTT GTCCGGGAAT TCTTCAGCGG GATTTCTCAC
    TCCATCATGT TTGAAGAACT TCCCAACGTG GATCGGATTC TGAAATTGTG CACAGACATT
    TTTTTAGTCC GGGAGACCGG GGAGCTGGAG CTCGAGGAGG ATCTCTATGC CAAATTAATA
    TTCCTGTACC GGTCGCCAGA AACCATGATC AAATGGACCA GGGAGAAAAC AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.