Piezo2 cDNA ORF clone, Mus musculus(house mouse)

The following Piezo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Piezo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu00667 NM_001039485.4
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54732 XM_006526166.4
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54733 XM_006526167.4
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54734 XM_006526168.4
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54735 XM_006526169.4
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54736 XM_006526170.4
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54738 XM_006526172.4
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54739 XM_006526173.4
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OMu54735 XM_006526169.3 Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54739 XM_006526173.3 Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54732 XM_006526166.3 Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54733 XM_006526167.3 Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54734 XM_006526168.3 Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54736 XM_006526170.3 Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu54738 XM_006526172.3 Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu00667
    Clone ID Related Accession (Same CDS sequence) NM_001039485.4
    Accession Version NM_001039485.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053702.1, HQ215521.1, AC140554.3 and BC075677.1. On Apr 14, 2011 this sequence version replaced NM_001039485.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HQ215521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGCCATGC CAAAGTCAAT
    GGTCGCGTGT ACCTAATAAT AAATAGGTTG GCCCACCCTG AAGGAAGTCT TCCAGACTTA
    GCCATCATGA ATATGACGGC CAGCCTTGAT AAGCCTGAGG TGCAGAAGTT GGCAGAGTCT
    GGGGAGGAGA GGCCAGAAGA GTGTGTCAAG AAGACGGAGA AGGGTGAGGC TGGGAAAGAC
    AGTGATGAGT CTGAGGAGGA GGAAGATGAA GAAGAGGAAT CCGAGGAAGA AGAAAGCTCA
    GACCTCAGGA ACAAGTGGCA CCTGGTGATT GACCGGCTCA CTGTGCTCTT TCTAAAATTC
    CTGGAGTATT TCCACAAGTT GCAGGTGTTT ATGTGGTGGA TCTTGGAGCT GCACATCATC
    AAAATCGTCT CATCGTACAT TATCTGGGTG ACTGTGAAAG AGGTGTCTCT GTTCAACTAT
    GTATTTTTGA TTTCTTGGGC ATTTGCTCTG CCATATGCCA AACTCCGTCG TGCGGCTTCC
    AGTGTCTGCA CTGTCTGGAC ATGTGTGATC ATCGTCTGCA AAATGTTGTA CCAGCTGCAA
    ACCATTAAAC CTGAGAACTT TTCTGTCAAC TGCTCCTTGC CCAATGAAAA TCAAACCAAC
    ATTCCCCTTC ATGAGTTGAA CAAGTCTCTT CTCTACAGTG CCCCTGTTGA TCCCACTGAG
    TGGGTAGGCC TGAGGAAGTC CTCTCCTTTG CTCGTCTACC TGAGGAACAA CCTCCTGATG
    CTGGCGATTT TGGCCTTTGA AGTCACCGTT TACCGCCATC AGGAGTACTA TCGAGGCCGA
    AACAACCTGA CTGCTCCAGT GTCTAAAACT ATCTTTCATG ACATCACAAG GTTACATCTA
    GATGATGGGC TTATCAATTG TGCCAAATAT TTTGTAAATT ACTTCTTCTA CAAGTTTGGC
    CTAGAGACCT GTTTCCTAAT GTCCGTGAAT GTAATTGGAC AGCGAATGGA CTTCTACGCC
    ATGATTCATG CCTGTTGGTT GATCGGTGTC TTGTATCGAC GCAGAAGAAA AGCCATTGCC
    GAGGTCTGGC CCAAGTACTG CTGCTTCCTG GCTTGCATCA TCACCTTCCA GTACTTCGTC
    TGCATCGGCA TCCCCCCTGC CCCTTGCCGA GATTATCCAT GGAGATTTAA GGGAGCCTAC
    TTCAATGATA ACATTATCAA GTGGCTGTAC TTCCCGGATT TCATTGTGCG GCCCAACCCC
    GTGTTTCTTG TCTATGACTT CATGCTGCTC CTGTGTGCTT CCTTACAACG ACAGATCTTT
    GAGGACGAAA ACAAGGCAGC CGTGCGCATC ATGGCCGGGG ACAATGTCGA GATCTGCATG
    AACCTTGATG CAGCCTCGTT CAGCCAGCAT AACCCTGTGC CAGATTTCAT TCACTGCAGA
    TCTTACTTGG ACATGTCCAA AGTGATCATC TTCAGCTACC TCTTCTGGTT CGTCCTCACC
    ATCATCTTCA TTACGGGAAC AACCAGGATC AGCATATTTT GCATGGGCTA CTTGGTGGCC
    TGTTTCTATT TCCTGCTCTT TGGAGGGGAT CTGCTGCTGA AGCCCATCAA GAGCATCCTT
    CGTTACTGGG ACTGGCTGAT TGCATACAAC GTGTTTGTGA TCACCATGAA GAATATACTG
    TCAATAGGAG CCTGTGGATA CATTGGCGCA CTAGTAAGAA ACAGCTGCTG GTTGATCCAA
    GCGTTCAGCC TGGCCTGCAC CGTGAAAGGC TATCAGATGC CTGAAGATGA CTCCAGATGC
    AAGCTGCCCA GTGGGGAGGC AGGGATCATC TGGGACAGCA TCTGCTTTGC GTTTCTGCTG
    CTGCAGAGGA GAGTCTTCAT GAGCTACTAC TTCCTGCATG TCGTGGCTGA CATTAAAGCA
    TCCCAGATCC TGGCATCCAG AGGGGCCGAA CTTTTCCAGG CTACAATTGT GAAAGCTGTA
    AAGGCAAGAA TTGAAGAAGA GAAAAAATCC ATGGACCAGC TGAAGAGACA GATGGATCGC
    ATCAAAGCCC GGCAACAAAA GTACAAAAAG GGTAAGGAGA GGATGCTGAG CTTGACCCAG
    GAGTCAGGGG AAGGCCAGGA CATCCAGAAA GTCTCAGAAG AAGATGATGA AAGAGAAGCA
    GACAAACAGA AAGCCAAGGG CAAAAAAAAG CAGTGGTGGC GGCCTTGGGT TGATCATGCT
    TCCATGGTCA GGAGTGGAGA TTATTATTTG TTTGAAACGG ATAGTGAAGA GGAGGAAGAA
    GAGGAACTTA AAAAGGAGGA TGAAGAACCC CCACGGAAGT CAGCATTCCA GTTCGTCTAC
    CAAGCCTGGA TTACTGATCC TAAAACGGCA CTCCGACAGA GGAGGAAAGA GAAGAAAAAG
    TTGGCCAGAG AAGAGCAGAA GGAGAGACGC AAAGGATCTG GGGACGGTCC AGTGGAATGG
    GAAGATCGAG AGGATGAGCC AGTCAAAAAG AAATCTGATG GACCAGATAA TATCATCAAG
    AGGATATTTA ATATCCTGAA GTTCACATGG GTTCTGTTCC TGGCCACAGT GGATAGTTTC
    ACCACTTGGC TCAACTCCAT CTCAAGGGAA CACATCGACA TATCCACAGT CCTGCGGATT
    GAAAGATGCA TGCTGACTCG AGAGATAAAA AAGGGCAATG TTCCAACACG GGAAAGCATC
    CACATGTACT ACCAGAACCA CATTATGAAC CTTTCCCGAG AATCCGGCCT GGACACAATC
    GACGAGCACT CGGGGGCTGG CTCCAGGGCC CAGGCAGCAC ATAGGATGGA TAGCTTAGAT
    TCCCGGGACA GTATCTCCAG CTGCTACACT GAAGCGACCC TGCTGATCTC AAGGCAGTCC
    ACCCTTGATG ATTTAGATGG ACAAGACCCC GTTCCTAAAA CAAGCGAGCG TGCTCGGCCT
    AGGCTCCGTA AAATGTTCAG CCTGGACATG TCCTCTTCGT CAGCTGACAG TGGCAGTGTA
    GCATCAAGCG AGCCCACACA GTGTACCATG CTGTATTCGC GCCAAGGAAC TACGGAAACC
    ATCGAGGAGG TGGAGGCTGA AGCTGAGGAG GAGGTGGTAG AAGGTCTGGA GCCTGAGCTC
    CATGATGCAG AAGAAAAGGA ATACGCAGCT GAATATGAAG CAGGGGTAGA GGAGATCAGC
    CTCACTCCAG ATGAGGAATT GCCTCAGTTC TCTACAGATG ACTGTGATGT GGAGGCGCCG
    CCCTCTTACA GCAAGGCTGT CAGCTTTGAG CATCTATCGT TTGCGTCGCA AGATGACTCT
    GGGGCCAAGA ACCACATGGT GGTCAGTCCT GATGACAGCC GTACAGACAA GCTGGAGTCA
    AGCATCTTAC CACCTCTGAC TCATGAGCTG ACAGCCAGCG ATCTGCTGAT GAGCAAGATG
    TTCCATGATG ATGAGCTTGA GGAGTCTGAG AAGTTCTACG TGGACCAGCC CCGGTTTTTG
    CTGCTATTCT ATGCCATGTA TAACACCCTG GTGGCCCGCT CAGAAATGGT GTGCTATTTC
    GTGATTATCC TGAACCACAT GACCTCTGCC TCCATCATCA CTCTCCTGTT GCCCATCTTG
    ATCTTCCTCT GGGCCATGCT GTCTGTTCCC AGGCCCAGCC GACGCTTCTG GATGATGGCC
    ATCGTCTACA CTGAGGTGGC GATCGTAGTG AAATACTTCT TCCAGTTCGG CTTCTTTCCC
    TGGAATAAGG ACTTGGAAAT ATACAAGGAA AGGCCCTACT TTCCACCGAA CATCATCGGG
    GTGGAGAAGA AGGAGGGCTA TGTTCTTTAT GACCTCATTC AGCTGCTGGC TCTATTCTTC
    CATCGCTCCA TTCTGAAGTG TCATGGTCTA TGGGACGAGG ATGACATTGT TGACAGTAAC
    ACAGACAAGG AAGGATCGGA TGACGAGCTG TCCCTCGATC AGGGCAGAAG AGGCTCCTCA
    GACTCACTGA AGTCTATCAA CCTGGCTGCC TCTGTAGAGT CTGTCCACGT GACCTTCCCC
    GAGCAGCCAG CGGCCATCAG GAGAAAACGC TCCTGCAGTA GCTCTCAGAT CTCCCCACGG
    TCCAGCTTCT CTTCAAATAG GTCCAAAAGA GGCAGCACAA GCACCCGGAA CAGTAGCCAG
    AAAGGAAGCA GTGTTTTAAG CCTTAAACAG AAAAGCAAAC GAGAACTTTA CATGGAAAAG
    CTTCAAGAGC ATTTAATCAA AGCAAAGGCG TTTACCATAA AGAAGACTCT ACAGATCTAT
    GTCCCCATTC GGCAATTCTT CTATGACCTC ATCCACCCAG ACTACAGTGC TGTGACTGAT
    GTATACGTGC TGATGTTTCT GGCTGACACT GTAGACTTCA TTATCATCGT CTTTGGATTC
    TGGGCCTTTG GGAAACACTC GGCAGCTGCA GATATCACCT CTTCTCTATC AGAAGACCAG
    GTCCCCGGGC CATTCCTGGT GATGGTCCTC ATTCAGTTTG GAACCATGGT GGTGGACCGT
    GCACTCTACC TCAGGAAGAC TGTGCTGGGC AAGGTCATCT TCCAGGTCAT CCTCGTGTTT
    GGGATTCACT TCTGGATGTT CTTCATCTTA CCCGGTGTGA CTGAGAGGAA ATTCAGCCAG
    AACCTGGTGG CACAGCTTTG GTACTTTGTG AAGTGTGTGT ACTTTGGGCT GTCTGCCTAT
    CAAATCCGCT GTGGCTACCC AACCCGTGTG CTCGGGAACT TCCTCACAAA GAGCTACAAT
    TACGTCAACC TCTTCTTGTT TCAAGGGTTC CGCCTGGTCC CATTTCTGAC TGAGCTGCGG
    GCTGTGATGG ATTGGGTGTG GACAGATACA ACCCTGAGCC TCTCCAGCTG GATTTGTGTG
    GAGGACATTT ACGCGCACAT TTTTATCTTA AAGTGCTGGC GAGAGTCGGA AAAGAGATAC
    CCTCAACCCA GGGGTCAGAA GAAGAAGAAA GCCGTGAAGT ACGGCATGGG CGGCATGATC
    ATCGTCCTTC TCATCTGTAT CGTCTGGTTT CCTCTGCTCT TCATGTCTTT GATCAAATCT
    GTCGCAGGGG TTATCAACCA ACCCCTGGAT GTGTCCGTCA CAATTACCCT GGGAGGTTAT
    CAGCCTATCT TCACAATGAG CGCCCAGCAA AGCCAGCTGA AAGTTATGGA TAATTCAAAG
    TATAATGAAT TTCTAAAATC TTTCGGCCCC AACAGTGGCG CAATGCAATT TCTGGAGAAC
    TATGAAAGGG AAGACGTAAC AGTAGCAGAA CTGGAAGGGA ACTCGAATTC TTTGTGGACC
    ATCAGCCCTC CCAGTAAGCA GAAAATGATA CAGGAGCTCA CCGACCCCAA TAGTTGCTTC
    TCTGTTGTTT TTTCATGGAG TATCCAGAGA AACATGACTT TGGGTGCAAA AGCCGAAATA
    GCAACGGATA AGCTTTCTTT CCCTCTTGCG GTCGCAACAC GGAATAGCAT AGCTAAAATG
    ATTGCTGGCA ATGACACAGA AAGTTCAAAC ACGCCAGTGA CAATAGAGAA GATCTACCCA
    TACTATGTGA AGGCACCCAG CGATTCAAAC TCAAAGCCTA TAAAACAGCT TCTGTCTGAA
    AATAATTTCA TGAATATCAC CATCATTTTG TTCAGAGACA ATGTCACAAA GTCCAATAGT
    GAGTGGTGGG TTCTCAACCT GACCGGAAGT AGGATATTCA ACCAGGGGTC CCAAGCCTTG
    GAACTGGTGG TCTTCAATGA CAAAGTCAGC CCTCCAAGTC TAGGCTTCTT GGCTGGCTAT
    GGTATCATGG GATTGTATGC ATCTGTTGTC CTTGTAATTG GGAAATTTGT TCGTGAGTTC
    TTCAGTGGGA TCTCTCATTC CATCATGTTT GAAGAGCTTC CAAATGTGGA CAGAATCTTG
    AAGTTGTGCA CAGATATATT CCTCGTGAGG GAGACAGGGG AGTTGGAACT GGAGGAAGAC
    CTCTACGCCA AGTTGATATT CCTGTACAGG TCACCAGAAA CCATGATCAA GTGGACTAGA
    GAAAAAACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001034574.4
    CDS915..9389
    Translation

    Target ORF information:

    RefSeq Version NM_001039485.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001039485.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGCCATGC CAAAGTCAAT
    GGTCGCGTGT ACCTAATAAT AAATAGGTTG GCCCACCCTG AAGGAAGTCT TCCAGACTTA
    GCCATCATGA ATATGACGGC CAGCCTTGAT AAGCCTGAGG TGCAGAAGTT GGCAGAGTCT
    GGGGAGGAGA GGCCAGAAGA GTGTGTCAAG AAGACGGAGA AGGGTGAGGC TGGGAAAGAC
    AGTGATGAGT CTGAGGAGGA GGAAGATGAA GAAGAGGAAT CCGAGGAAGA AGAAAGCTCA
    GACCTCAGGA ACAAGTGGCA CCTGGTGATT GACCGGCTCA CTGTGCTCTT TCTAAAATTC
    CTGGAGTATT TCCACAAGTT GCAGGTGTTT ATGTGGTGGA TCTTGGAGCT GCACATCATC
    AAAATCGTCT CATCGTACAT TATCTGGGTG ACTGTGAAAG AGGTGTCTCT GTTCAACTAT
    GTATTTTTGA TTTCTTGGGC ATTTGCTCTG CCATATGCCA AACTCCGTCG TGCGGCTTCC
    AGTGTCTGCA CTGTCTGGAC ATGTGTGATC ATCGTCTGCA AAATGTTGTA CCAGCTGCAA
    ACCATTAAAC CTGAGAACTT TTCTGTCAAC TGCTCCTTGC CCAATGAAAA TCAAACCAAC
    ATTCCCCTTC ATGAGTTGAA CAAGTCTCTT CTCTACAGTG CCCCTGTTGA TCCCACTGAG
    TGGGTAGGCC TGAGGAAGTC CTCTCCTTTG CTCGTCTACC TGAGGAACAA CCTCCTGATG
    CTGGCGATTT TGGCCTTTGA AGTCACCGTT TACCGCCATC AGGAGTACTA TCGAGGCCGA
    AACAACCTGA CTGCTCCAGT GTCTAAAACT ATCTTTCATG ACATCACAAG GTTACATCTA
    GATGATGGGC TTATCAATTG TGCCAAATAT TTTGTAAATT ACTTCTTCTA CAAGTTTGGC
    CTAGAGACCT GTTTCCTAAT GTCCGTGAAT GTAATTGGAC AGCGAATGGA CTTCTACGCC
    ATGATTCATG CCTGTTGGTT GATCGGTGTC TTGTATCGAC GCAGAAGAAA AGCCATTGCC
    GAGGTCTGGC CCAAGTACTG CTGCTTCCTG GCTTGCATCA TCACCTTCCA GTACTTCGTC
    TGCATCGGCA TCCCCCCTGC CCCTTGCCGA GATTATCCAT GGAGATTTAA GGGAGCCTAC
    TTCAATGATA ACATTATCAA GTGGCTGTAC TTCCCGGATT TCATTGTGCG GCCCAACCCC
    GTGTTTCTTG TCTATGACTT CATGCTGCTC CTGTGTGCTT CCTTACAACG ACAGATCTTT
    GAGGACGAAA ACAAGGCAGC CGTGCGCATC ATGGCCGGGG ACAATGTCGA GATCTGCATG
    AACCTTGATG CAGCCTCGTT CAGCCAGCAT AACCCTGTGC CAGATTTCAT TCACTGCAGA
    TCTTACTTGG ACATGTCCAA AGTGATCATC TTCAGCTACC TCTTCTGGTT CGTCCTCACC
    ATCATCTTCA TTACGGGAAC AACCAGGATC AGCATATTTT GCATGGGCTA CTTGGTGGCC
    TGTTTCTATT TCCTGCTCTT TGGAGGGGAT CTGCTGCTGA AGCCCATCAA GAGCATCCTT
    CGTTACTGGG ACTGGCTGAT TGCATACAAC GTGTTTGTGA TCACCATGAA GAATATACTG
    TCAATAGGAG CCTGTGGATA CATTGGCGCA CTAGTAAGAA ACAGCTGCTG GTTGATCCAA
    GCGTTCAGCC TGGCCTGCAC CGTGAAAGGC TATCAGATGC CTGAAGATGA CTCCAGATGC
    AAGCTGCCCA GTGGGGAGGC AGGGATCATC TGGGACAGCA TCTGCTTTGC GTTTCTGCTG
    CTGCAGAGGA GAGTCTTCAT GAGCTACTAC TTCCTGCATG TCGTGGCTGA CATTAAAGCA
    TCCCAGATCC TGGCATCCAG AGGGGCCGAA CTTTTCCAGG CTACAATTGT GAAAGCTGTA
    AAGGCAAGAA TTGAAGAAGA GAAAAAATCC ATGGACCAGC TGAAGAGACA GATGGATCGC
    ATCAAAGCCC GGCAACAAAA GTACAAAAAG GGTAAGGAGA GGATGCTGAG CTTGACCCAG
    GAGTCAGGGG AAGGCCAGGA CATCCAGAAA GTCTCAGAAG AAGATGATGA AAGAGAAGCA
    GACAAACAGA AAGCCAAGGG CAAAAAAAAG CAGTGGTGGC GGCCTTGGGT TGATCATGCT
    TCCATGGTCA GGAGTGGAGA TTATTATTTG TTTGAAACGG ATAGTGAAGA GGAGGAAGAA
    GAGGAACTTA AAAAGGAGGA TGAAGAACCC CCACGGAAGT CAGCATTCCA GTTCGTCTAC
    CAAGCCTGGA TTACTGATCC TAAAACGGCA CTCCGACAGA GGAGGAAAGA GAAGAAAAAG
    TTGGCCAGAG AAGAGCAGAA GGAGAGACGC AAAGGATCTG GGGACGGTCC AGTGGAATGG
    GAAGATCGAG AGGATGAGCC AGTCAAAAAG AAATCTGATG GACCAGATAA TATCATCAAG
    AGGATATTTA ATATCCTGAA GTTCACATGG GTTCTGTTCC TGGCCACAGT GGATAGTTTC
    ACCACTTGGC TCAACTCCAT CTCAAGGGAA CACATCGACA TATCCACAGT CCTGCGGATT
    GAAAGATGCA TGCTGACTCG AGAGATAAAA AAGGGCAATG TTCCAACACG GGAAAGCATC
    CACATGTACT ACCAGAACCA CATTATGAAC CTTTCCCGAG AATCCGGCCT GGACACAATC
    GACGAGCACT CGGGGGCTGG CTCCAGGGCC CAGGCAGCAC ATAGGATGGA TAGCTTAGAT
    TCCCGGGACA GTATCTCCAG CTGCTACACT GAAGCGACCC TGCTGATCTC AAGGCAGTCC
    ACCCTTGATG ATTTAGATGG ACAAGACCCC GTTCCTAAAA CAAGCGAGCG TGCTCGGCCT
    AGGCTCCGTA AAATGTTCAG CCTGGACATG TCCTCTTCGT CAGCTGACAG TGGCAGTGTA
    GCATCAAGCG AGCCCACACA GTGTACCATG CTGTATTCGC GCCAAGGAAC TACGGAAACC
    ATCGAGGAGG TGGAGGCTGA AGCTGAGGAG GAGGTGGTAG AAGGTCTGGA GCCTGAGCTC
    CATGATGCAG AAGAAAAGGA ATACGCAGCT GAATATGAAG CAGGGGTAGA GGAGATCAGC
    CTCACTCCAG ATGAGGAATT GCCTCAGTTC TCTACAGATG ACTGTGATGT GGAGGCGCCG
    CCCTCTTACA GCAAGGCTGT CAGCTTTGAG CATCTATCGT TTGCGTCGCA AGATGACTCT
    GGGGCCAAGA ACCACATGGT GGTCAGTCCT GATGACAGCC GTACAGACAA GCTGGAGTCA
    AGCATCTTAC CACCTCTGAC TCATGAGCTG ACAGCCAGCG ATCTGCTGAT GAGCAAGATG
    TTCCATGATG ATGAGCTTGA GGAGTCTGAG AAGTTCTACG TGGACCAGCC CCGGTTTTTG
    CTGCTATTCT ATGCCATGTA TAACACCCTG GTGGCCCGCT CAGAAATGGT GTGCTATTTC
    GTGATTATCC TGAACCACAT GACCTCTGCC TCCATCATCA CTCTCCTGTT GCCCATCTTG
    ATCTTCCTCT GGGCCATGCT GTCTGTTCCC AGGCCCAGCC GACGCTTCTG GATGATGGCC
    ATCGTCTACA CTGAGGTGGC GATCGTAGTG AAATACTTCT TCCAGTTCGG CTTCTTTCCC
    TGGAATAAGG ACTTGGAAAT ATACAAGGAA AGGCCCTACT TTCCACCGAA CATCATCGGG
    GTGGAGAAGA AGGAGGGCTA TGTTCTTTAT GACCTCATTC AGCTGCTGGC TCTATTCTTC
    CATCGCTCCA TTCTGAAGTG TCATGGTCTA TGGGACGAGG ATGACATTGT TGACAGTAAC
    ACAGACAAGG AAGGATCGGA TGACGAGCTG TCCCTCGATC AGGGCAGAAG AGGCTCCTCA
    GACTCACTGA AGTCTATCAA CCTGGCTGCC TCTGTAGAGT CTGTCCACGT GACCTTCCCC
    GAGCAGCCAG CGGCCATCAG GAGAAAACGC TCCTGCAGTA GCTCTCAGAT CTCCCCACGG
    TCCAGCTTCT CTTCAAATAG GTCCAAAAGA GGCAGCACAA GCACCCGGAA CAGTAGCCAG
    AAAGGAAGCA GTGTTTTAAG CCTTAAACAG AAAAGCAAAC GAGAACTTTA CATGGAAAAG
    CTTCAAGAGC ATTTAATCAA AGCAAAGGCG TTTACCATAA AGAAGACTCT ACAGATCTAT
    GTCCCCATTC GGCAATTCTT CTATGACCTC ATCCACCCAG ACTACAGTGC TGTGACTGAT
    GTATACGTGC TGATGTTTCT GGCTGACACT GTAGACTTCA TTATCATCGT CTTTGGATTC
    TGGGCCTTTG GGAAACACTC GGCAGCTGCA GATATCACCT CTTCTCTATC AGAAGACCAG
    GTCCCCGGGC CATTCCTGGT GATGGTCCTC ATTCAGTTTG GAACCATGGT GGTGGACCGT
    GCACTCTACC TCAGGAAGAC TGTGCTGGGC AAGGTCATCT TCCAGGTCAT CCTCGTGTTT
    GGGATTCACT TCTGGATGTT CTTCATCTTA CCCGGTGTGA CTGAGAGGAA ATTCAGCCAG
    AACCTGGTGG CACAGCTTTG GTACTTTGTG AAGTGTGTGT ACTTTGGGCT GTCTGCCTAT
    CAAATCCGCT GTGGCTACCC AACCCGTGTG CTCGGGAACT TCCTCACAAA GAGCTACAAT
    TACGTCAACC TCTTCTTGTT TCAAGGGTTC CGCCTGGTCC CATTTCTGAC TGAGCTGCGG
    GCTGTGATGG ATTGGGTGTG GACAGATACA ACCCTGAGCC TCTCCAGCTG GATTTGTGTG
    GAGGACATTT ACGCGCACAT TTTTATCTTA AAGTGCTGGC GAGAGTCGGA AAAGAGATAC
    CCTCAACCCA GGGGTCAGAA GAAGAAGAAA GCCGTGAAGT ACGGCATGGG CGGCATGATC
    ATCGTCCTTC TCATCTGTAT CGTCTGGTTT CCTCTGCTCT TCATGTCTTT GATCAAATCT
    GTCGCAGGGG TTATCAACCA ACCCCTGGAT GTGTCCGTCA CAATTACCCT GGGAGGTTAT
    CAGCCTATCT TCACAATGAG CGCCCAGCAA AGCCAGCTGA AAGTTATGGA TAATTCAAAG
    TATAATGAAT TTCTAAAATC TTTCGGCCCC AACAGTGGCG CAATGCAATT TCTGGAGAAC
    TATGAAAGGG AAGACGTAAC AGTAGCAGAA CTGGAAGGGA ACTCGAATTC TTTGTGGACC
    ATCAGCCCTC CCAGTAAGCA GAAAATGATA CAGGAGCTCA CCGACCCCAA TAGTTGCTTC
    TCTGTTGTTT TTTCATGGAG TATCCAGAGA AACATGACTT TGGGTGCAAA AGCCGAAATA
    GCAACGGATA AGCTTTCTTT CCCTCTTGCG GTCGCAACAC GGAATAGCAT AGCTAAAATG
    ATTGCTGGCA ATGACACAGA AAGTTCAAAC ACGCCAGTGA CAATAGAGAA GATCTACCCA
    TACTATGTGA AGGCACCCAG CGATTCAAAC TCAAAGCCTA TAAAACAGCT TCTGTCTGAA
    AATAATTTCA TGAATATCAC CATCATTTTG TTCAGAGACA ATGTCACAAA GTCCAATAGT
    GAGTGGTGGG TTCTCAACCT GACCGGAAGT AGGATATTCA ACCAGGGGTC CCAAGCCTTG
    GAACTGGTGG TCTTCAATGA CAAAGTCAGC CCTCCAAGTC TAGGCTTCTT GGCTGGCTAT
    GGTATCATGG GATTGTATGC ATCTGTTGTC CTTGTAATTG GGAAATTTGT TCGTGAGTTC
    TTCAGTGGGA TCTCTCATTC CATCATGTTT GAAGAGCTTC CAAATGTGGA CAGAATCTTG
    AAGTTGTGCA CAGATATATT CCTCGTGAGG GAGACAGGGG AGTTGGAACT GGAGGAAGAC
    CTCTACGCCA AGTTGATATT CCTGTACAGG TCACCAGAAA CCATGATCAA GTGGACTAGA
    GAAAAAACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54732
    Clone ID Related Accession (Same CDS sequence) XM_006526166.4 , XM_006526166.3
    Accession Version XM_006526166.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526166.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGA GAGCTATTGG GAAGTTTGCC TCAGCCATTT TAGCCTTGCC AAAGTCTGTC
    ATTAAACTTC CCAAAACTAT TCTTCAGTAT TTAATCAGAG CTGCAAAGTT CGTCTACCAA
    GCCTGGATTA CTGATCCTAA AACGGCACTC CGACAGAGGA GGAAAGAGAA GAAAAAGTTG
    GCCAGAGAAG AGCAGAAGGA GAGACGCAAA GGATCTGGGG ACGGTCCAGT GGAATGGGAA
    GATCGAGAGG ATGAGCCAGT CAAAAAGAAA TCTGATGGAC CAGATAATAT CATCAAGAGG
    ATATTTAATA TCCTGAAGTT CACATGGGTT CTGTTCCTGG CCACAGTGGA TAGTTTCACC
    ACTTGGCTCA ACTCCATCTC AAGGGAACAC ATCGACATAT CCACAGTCCT GCGGATTGAA
    AGATGCATGC TGACTCGAGA GATAAAAAAG GGCAATGTTC CAACACGGGA AAGCATCCAC
    ATGTACTACC AGAACCACAT TATGAACCTT TCCCGAGAAT CCGGCCTGGA CACAATCGAC
    GAGCACTCGG GGGCTGGCTC CAGGGCCCAG GCAGCACATA GGATGGATAG CTTAGATTCC
    CGGGACAGTA TCTCCAGCTG CTACACTGAA GCGACCCTGC TGATCTCAAG GCAGTCCACC
    CTTGATGATT TAGATGGACA AGACCCCGTT CCTAAAACAA GCGAGCGTGC TCGGCCTAGG
    CTCCGTAAAA TGTTCAGCCT GGACATGTCC TCTTCGTCAG CTGACAGTGG CAGTGTAGCA
    TCAAGCGAGC CCACACAGTG TACCATGCTG TATTCGCGCC AAGGAACTAC GGAAACCATC
    GAGGAGGTGG AGGCTGAAGC TGAGGAGGAG GTGGTAGAAG GTCTGGAGCC TGAGCTCCAT
    GATGCAGAAG AAAAGGAATA CGCAGCTGAA TATGAAGCAG GGGTAGAGGA GATCAGCCTC
    ACTCCAGATG AGGAATTGCC TCAGTTCTCT ACAGATGACT GTGATGTGGA GGCGCCGCCC
    TCTTACAGCA AGGCTGTCAG CTTTGAGCAT CTATCGTTTG CGTCGCAAGA TGACTCTGGG
    GCCAAGAACC ACATGGTGGT CAGTCCTGAT GACAGCCGTA CAGACAAGCT GGAGTCAAGC
    ATCTTACCAC CTCTGACTCA TGAGCTGACA GCCAGCGATC TGCTGATGAG CAAGATGTTC
    CATGATGATG AGCTTGAGGA GTCTGAGAAG TTCTACGTGG ACCAGCCCCG GTTTTTGCTG
    CTATTCTATG CCATGTATAA CACCCTGGTG GCCCGCTCAG AAATGGTGTG CTATTTCGTG
    ATTATCCTGA ACCACATGAC CTCTGCCTCC ATCATCACTC TCCTGTTGCC CATCTTGATC
    TTCCTCTGGG CCATGCTGTC TGTTCCCAGG CCCAGCCGAC GCTTCTGGAT GATGGCCATC
    GTCTACACTG AGGTGGCGAT CGTAGTGAAA TACTTCTTCC AGTTCGGCTT CTTTCCCTGG
    AATAAGGACT TGGAAATATA CAAGGAAAGG CCCTACTTTC CACCGAACAT CATCGGGGTG
    GAGAAGAAGG AGGGCTATGT TCTTTATGAC CTCATTCAGC TGCTGGCTCT ATTCTTCCAT
    CGCTCCATTC TGAAGTGTCA TGGTCTATGG GACGAGGATG ACATTGTTGA CAGTAACACA
    GACAAGGAAG GATCGGATGA CGAGCTGTCC CTCGATCAGG GCAGAAGAGG CTCCTCAGAC
    TCACTGAAGT CTATCAACCT GGCTGCCTCT GTAGAGTCTG TCCACGTGAC CTTCCCCGAG
    CAGCCAGCGG CCATCAGGAG AAAACGCTCC TGCAGTAGCT CTCAGATCTC CCCACGGTCC
    AGCTTCTCTT CAAATAGGTC CAAAAGAGGC AGCACAAGCA CCCGGAACAG TAGCCAGAAA
    GGAAGCAGTG TTTTAAGCCT TAAACAGAAA AGCAAACGAG AACTTTACAT GGAAAAGCTT
    CAAGAGCATT TAATCAAAGC AAAGGCGTTT ACCATAAAGA AGACTCTACA GATCTATGTC
    CCCATTCGGC AATTCTTCTA TGACCTCATC CACCCAGACT ACAGTGCTGT GACTGATGTA
    TACGTGCTGA TGTTTCTGGC TGACACTGTA GACTTCATTA TCATCGTCTT TGGATTCTGG
    GCCTTTGGGA AACACTCGGC AGCTGCAGAT ATCACCTCTT CTCTATCAGA AGACCAGGTC
    CCCGGGCCAT TCCTGGTGAT GGTCCTCATT CAGTTTGGAA CCATGGTGGT GGACCGTGCA
    CTCTACCTCA GGAAGACTGT GCTGGGCAAG GTCATCTTCC AGGTCATCCT CGTGTTTGGG
    ATTCACTTCT GGATGTTCTT CATCTTACCC GGTGTGACTG AGAGGAAATT CAGCCAGAAC
    CTGGTGGCAC AGCTTTGGTA CTTTGTGAAG TGTGTGTACT TTGGGCTGTC TGCCTATCAA
    ATCCGCTGTG GCTACCCAAC CCGTGTGCTC GGGAACTTCC TCACAAAGAG CTACAATTAC
    GTCAACCTCT TCTTGTTTCA AGGGTTCCGC CTGGTCCCAT TTCTGACTGA GCTGCGGGCT
    GTGATGGATT GGGTGTGGAC AGATACAACC CTGAGCCTCT CCAGCTGGAT TTGTGTGGAG
    GACATTTACG CGCACATTTT TATCTTAAAG TGCTGGCGAG AGTCGGAAAA GAGATACCCT
    CAACCCAGGG GTCAGAAGAA GAAGAAAGCC GTGAAGTACG GCATGGGCGG CATGATCATC
    GTCCTTCTCA TCTGTATCGT CTGGTTTCCT CTGCTCTTCA TGTCTTTGAT CAAATCTGTC
    GCAGGGGTTA TCAACCAACC CCTGGATGTG TCCGTCACAA TTACCCTGGG AGGTTATCAG
    CCTATCTTCA CAATGAGCGC CCAGCAAAGC CAGCTGAAAG TTATGGATAA TTCAAAGTAT
    AATGAATTTC TAAAATCTTT CGGCCCCAAC AGTGGCGCAA TGCAATTTCT GGAGAACTAT
    GAAAGGGAAG ACGTAACAGT AGCAGAACTG GAAGGGAACT CGAATTCTTT GTGGACCATC
    AGCCCTCCCA GTAAGCAGAA AATGATACAG GAGCTCACCG ACCCCAATAG TTGCTTCTCT
    GTTGTTTTTT CATGGAGTAT CCAGAGAAAC ATGACTTTGG GTGCAAAAGC CGAAATAGCA
    ACGGATAAGC TTTCTTTCCC TCTTGCGGTC GCAACACGGA ATAGCATAGC TAAAATGATT
    GCTGGCAATG ACACAGAAAG TTCAAACACG CCAGTGACAA TAGAGAAGAT CTACCCATAC
    TATGTGAAGG CACCCAGCGA TTCAAACTCA AAGCCTATAA AACAGCTTCT GTCTGAAAAT
    AATTTCATGA ATATCACCAT CATTTTGTTC AGAGACAATG TCACAAAGTC CAATAGTGAG
    TGGTGGGTTC TCAACCTGAC CGGAAGTAGG ATATTCAACC AGGGGTCCCA AGCCTTGGAA
    CTGGTGGTCT TCAATGACAA AGTCAGCCCT CCAAGTCTAG GCTTCTTGGC TGGCTATGGT
    ATCATGGGAT TGTATGCATC TGTTGTCCTT GTAATTGGGA AATTTGTTCG TGAGTTCTTC
    AGTGGGATCT CTCATTCCAT CATGTTTGAA GAGCTTCCAA ATGTGGACAG AATCTTGAAG
    TTGTGCACAG ATATATTCCT CGTGAGGGAG ACAGGGGAGT TGGAACTGGA GGAAGACCTC
    TACGCCAAGT TGATATTCCT GTACAGGTCA CCAGAAACCA TGATCAAGTG GACTAGAGAA
    AAAACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526229.1
    CDS1448..9979
    Translation

    Target ORF information:

    RefSeq Version XM_006526166.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526166.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGA GAGCTATTGG GAAGTTTGCC TCAGCCATTT TAGCCTTGCC AAAGTCTGTC
    ATTAAACTTC CCAAAACTAT TCTTCAGTAT TTAATCAGAG CTGCAAAGTT CGTCTACCAA
    GCCTGGATTA CTGATCCTAA AACGGCACTC CGACAGAGGA GGAAAGAGAA GAAAAAGTTG
    GCCAGAGAAG AGCAGAAGGA GAGACGCAAA GGATCTGGGG ACGGTCCAGT GGAATGGGAA
    GATCGAGAGG ATGAGCCAGT CAAAAAGAAA TCTGATGGAC CAGATAATAT CATCAAGAGG
    ATATTTAATA TCCTGAAGTT CACATGGGTT CTGTTCCTGG CCACAGTGGA TAGTTTCACC
    ACTTGGCTCA ACTCCATCTC AAGGGAACAC ATCGACATAT CCACAGTCCT GCGGATTGAA
    AGATGCATGC TGACTCGAGA GATAAAAAAG GGCAATGTTC CAACACGGGA AAGCATCCAC
    ATGTACTACC AGAACCACAT TATGAACCTT TCCCGAGAAT CCGGCCTGGA CACAATCGAC
    GAGCACTCGG GGGCTGGCTC CAGGGCCCAG GCAGCACATA GGATGGATAG CTTAGATTCC
    CGGGACAGTA TCTCCAGCTG CTACACTGAA GCGACCCTGC TGATCTCAAG GCAGTCCACC
    CTTGATGATT TAGATGGACA AGACCCCGTT CCTAAAACAA GCGAGCGTGC TCGGCCTAGG
    CTCCGTAAAA TGTTCAGCCT GGACATGTCC TCTTCGTCAG CTGACAGTGG CAGTGTAGCA
    TCAAGCGAGC CCACACAGTG TACCATGCTG TATTCGCGCC AAGGAACTAC GGAAACCATC
    GAGGAGGTGG AGGCTGAAGC TGAGGAGGAG GTGGTAGAAG GTCTGGAGCC TGAGCTCCAT
    GATGCAGAAG AAAAGGAATA CGCAGCTGAA TATGAAGCAG GGGTAGAGGA GATCAGCCTC
    ACTCCAGATG AGGAATTGCC TCAGTTCTCT ACAGATGACT GTGATGTGGA GGCGCCGCCC
    TCTTACAGCA AGGCTGTCAG CTTTGAGCAT CTATCGTTTG CGTCGCAAGA TGACTCTGGG
    GCCAAGAACC ACATGGTGGT CAGTCCTGAT GACAGCCGTA CAGACAAGCT GGAGTCAAGC
    ATCTTACCAC CTCTGACTCA TGAGCTGACA GCCAGCGATC TGCTGATGAG CAAGATGTTC
    CATGATGATG AGCTTGAGGA GTCTGAGAAG TTCTACGTGG ACCAGCCCCG GTTTTTGCTG
    CTATTCTATG CCATGTATAA CACCCTGGTG GCCCGCTCAG AAATGGTGTG CTATTTCGTG
    ATTATCCTGA ACCACATGAC CTCTGCCTCC ATCATCACTC TCCTGTTGCC CATCTTGATC
    TTCCTCTGGG CCATGCTGTC TGTTCCCAGG CCCAGCCGAC GCTTCTGGAT GATGGCCATC
    GTCTACACTG AGGTGGCGAT CGTAGTGAAA TACTTCTTCC AGTTCGGCTT CTTTCCCTGG
    AATAAGGACT TGGAAATATA CAAGGAAAGG CCCTACTTTC CACCGAACAT CATCGGGGTG
    GAGAAGAAGG AGGGCTATGT TCTTTATGAC CTCATTCAGC TGCTGGCTCT ATTCTTCCAT
    CGCTCCATTC TGAAGTGTCA TGGTCTATGG GACGAGGATG ACATTGTTGA CAGTAACACA
    GACAAGGAAG GATCGGATGA CGAGCTGTCC CTCGATCAGG GCAGAAGAGG CTCCTCAGAC
    TCACTGAAGT CTATCAACCT GGCTGCCTCT GTAGAGTCTG TCCACGTGAC CTTCCCCGAG
    CAGCCAGCGG CCATCAGGAG AAAACGCTCC TGCAGTAGCT CTCAGATCTC CCCACGGTCC
    AGCTTCTCTT CAAATAGGTC CAAAAGAGGC AGCACAAGCA CCCGGAACAG TAGCCAGAAA
    GGAAGCAGTG TTTTAAGCCT TAAACAGAAA AGCAAACGAG AACTTTACAT GGAAAAGCTT
    CAAGAGCATT TAATCAAAGC AAAGGCGTTT ACCATAAAGA AGACTCTACA GATCTATGTC
    CCCATTCGGC AATTCTTCTA TGACCTCATC CACCCAGACT ACAGTGCTGT GACTGATGTA
    TACGTGCTGA TGTTTCTGGC TGACACTGTA GACTTCATTA TCATCGTCTT TGGATTCTGG
    GCCTTTGGGA AACACTCGGC AGCTGCAGAT ATCACCTCTT CTCTATCAGA AGACCAGGTC
    CCCGGGCCAT TCCTGGTGAT GGTCCTCATT CAGTTTGGAA CCATGGTGGT GGACCGTGCA
    CTCTACCTCA GGAAGACTGT GCTGGGCAAG GTCATCTTCC AGGTCATCCT CGTGTTTGGG
    ATTCACTTCT GGATGTTCTT CATCTTACCC GGTGTGACTG AGAGGAAATT CAGCCAGAAC
    CTGGTGGCAC AGCTTTGGTA CTTTGTGAAG TGTGTGTACT TTGGGCTGTC TGCCTATCAA
    ATCCGCTGTG GCTACCCAAC CCGTGTGCTC GGGAACTTCC TCACAAAGAG CTACAATTAC
    GTCAACCTCT TCTTGTTTCA AGGGTTCCGC CTGGTCCCAT TTCTGACTGA GCTGCGGGCT
    GTGATGGATT GGGTGTGGAC AGATACAACC CTGAGCCTCT CCAGCTGGAT TTGTGTGGAG
    GACATTTACG CGCACATTTT TATCTTAAAG TGCTGGCGAG AGTCGGAAAA GAGATACCCT
    CAACCCAGGG GTCAGAAGAA GAAGAAAGCC GTGAAGTACG GCATGGGCGG CATGATCATC
    GTCCTTCTCA TCTGTATCGT CTGGTTTCCT CTGCTCTTCA TGTCTTTGAT CAAATCTGTC
    GCAGGGGTTA TCAACCAACC CCTGGATGTG TCCGTCACAA TTACCCTGGG AGGTTATCAG
    CCTATCTTCA CAATGAGCGC CCAGCAAAGC CAGCTGAAAG TTATGGATAA TTCAAAGTAT
    AATGAATTTC TAAAATCTTT CGGCCCCAAC AGTGGCGCAA TGCAATTTCT GGAGAACTAT
    GAAAGGGAAG ACGTAACAGT AGCAGAACTG GAAGGGAACT CGAATTCTTT GTGGACCATC
    AGCCCTCCCA GTAAGCAGAA AATGATACAG GAGCTCACCG ACCCCAATAG TTGCTTCTCT
    GTTGTTTTTT CATGGAGTAT CCAGAGAAAC ATGACTTTGG GTGCAAAAGC CGAAATAGCA
    ACGGATAAGC TTTCTTTCCC TCTTGCGGTC GCAACACGGA ATAGCATAGC TAAAATGATT
    GCTGGCAATG ACACAGAAAG TTCAAACACG CCAGTGACAA TAGAGAAGAT CTACCCATAC
    TATGTGAAGG CACCCAGCGA TTCAAACTCA AAGCCTATAA AACAGCTTCT GTCTGAAAAT
    AATTTCATGA ATATCACCAT CATTTTGTTC AGAGACAATG TCACAAAGTC CAATAGTGAG
    TGGTGGGTTC TCAACCTGAC CGGAAGTAGG ATATTCAACC AGGGGTCCCA AGCCTTGGAA
    CTGGTGGTCT TCAATGACAA AGTCAGCCCT CCAAGTCTAG GCTTCTTGGC TGGCTATGGT
    ATCATGGGAT TGTATGCATC TGTTGTCCTT GTAATTGGGA AATTTGTTCG TGAGTTCTTC
    AGTGGGATCT CTCATTCCAT CATGTTTGAA GAGCTTCCAA ATGTGGACAG AATCTTGAAG
    TTGTGCACAG ATATATTCCT CGTGAGGGAG ACAGGGGAGT TGGAACTGGA GGAAGACCTC
    TACGCCAAGT TGATATTCCT GTACAGGTCA CCAGAAACCA TGATCAAGTG GACTAGAGAA
    AAAACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54732
    Clone ID Related Accession (Same CDS sequence) XM_006526166.4 , XM_006526166.3
    Accession Version XM_006526166.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526166.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGA GAGCTATTGG GAAGTTTGCC TCAGCCATTT TAGCCTTGCC AAAGTCTGTC
    ATTAAACTTC CCAAAACTAT TCTTCAGTAT TTAATCAGAG CTGCAAAGTT CGTCTACCAA
    GCCTGGATTA CTGATCCTAA AACGGCACTC CGACAGAGGA GGAAAGAGAA GAAAAAGTTG
    GCCAGAGAAG AGCAGAAGGA GAGACGCAAA GGATCTGGGG ACGGTCCAGT GGAATGGGAA
    GATCGAGAGG ATGAGCCAGT CAAAAAGAAA TCTGATGGAC CAGATAATAT CATCAAGAGG
    ATATTTAATA TCCTGAAGTT CACATGGGTT CTGTTCCTGG CCACAGTGGA TAGTTTCACC
    ACTTGGCTCA ACTCCATCTC AAGGGAACAC ATCGACATAT CCACAGTCCT GCGGATTGAA
    AGATGCATGC TGACTCGAGA GATAAAAAAG GGCAATGTTC CAACACGGGA AAGCATCCAC
    ATGTACTACC AGAACCACAT TATGAACCTT TCCCGAGAAT CCGGCCTGGA CACAATCGAC
    GAGCACTCGG GGGCTGGCTC CAGGGCCCAG GCAGCACATA GGATGGATAG CTTAGATTCC
    CGGGACAGTA TCTCCAGCTG CTACACTGAA GCGACCCTGC TGATCTCAAG GCAGTCCACC
    CTTGATGATT TAGATGGACA AGACCCCGTT CCTAAAACAA GCGAGCGTGC TCGGCCTAGG
    CTCCGTAAAA TGTTCAGCCT GGACATGTCC TCTTCGTCAG CTGACAGTGG CAGTGTAGCA
    TCAAGCGAGC CCACACAGTG TACCATGCTG TATTCGCGCC AAGGAACTAC GGAAACCATC
    GAGGAGGTGG AGGCTGAAGC TGAGGAGGAG GTGGTAGAAG GTCTGGAGCC TGAGCTCCAT
    GATGCAGAAG AAAAGGAATA CGCAGCTGAA TATGAAGCAG GGGTAGAGGA GATCAGCCTC
    ACTCCAGATG AGGAATTGCC TCAGTTCTCT ACAGATGACT GTGATGTGGA GGCGCCGCCC
    TCTTACAGCA AGGCTGTCAG CTTTGAGCAT CTATCGTTTG CGTCGCAAGA TGACTCTGGG
    GCCAAGAACC ACATGGTGGT CAGTCCTGAT GACAGCCGTA CAGACAAGCT GGAGTCAAGC
    ATCTTACCAC CTCTGACTCA TGAGCTGACA GCCAGCGATC TGCTGATGAG CAAGATGTTC
    CATGATGATG AGCTTGAGGA GTCTGAGAAG TTCTACGTGG ACCAGCCCCG GTTTTTGCTG
    CTATTCTATG CCATGTATAA CACCCTGGTG GCCCGCTCAG AAATGGTGTG CTATTTCGTG
    ATTATCCTGA ACCACATGAC CTCTGCCTCC ATCATCACTC TCCTGTTGCC CATCTTGATC
    TTCCTCTGGG CCATGCTGTC TGTTCCCAGG CCCAGCCGAC GCTTCTGGAT GATGGCCATC
    GTCTACACTG AGGTGGCGAT CGTAGTGAAA TACTTCTTCC AGTTCGGCTT CTTTCCCTGG
    AATAAGGACT TGGAAATATA CAAGGAAAGG CCCTACTTTC CACCGAACAT CATCGGGGTG
    GAGAAGAAGG AGGGCTATGT TCTTTATGAC CTCATTCAGC TGCTGGCTCT ATTCTTCCAT
    CGCTCCATTC TGAAGTGTCA TGGTCTATGG GACGAGGATG ACATTGTTGA CAGTAACACA
    GACAAGGAAG GATCGGATGA CGAGCTGTCC CTCGATCAGG GCAGAAGAGG CTCCTCAGAC
    TCACTGAAGT CTATCAACCT GGCTGCCTCT GTAGAGTCTG TCCACGTGAC CTTCCCCGAG
    CAGCCAGCGG CCATCAGGAG AAAACGCTCC TGCAGTAGCT CTCAGATCTC CCCACGGTCC
    AGCTTCTCTT CAAATAGGTC CAAAAGAGGC AGCACAAGCA CCCGGAACAG TAGCCAGAAA
    GGAAGCAGTG TTTTAAGCCT TAAACAGAAA AGCAAACGAG AACTTTACAT GGAAAAGCTT
    CAAGAGCATT TAATCAAAGC AAAGGCGTTT ACCATAAAGA AGACTCTACA GATCTATGTC
    CCCATTCGGC AATTCTTCTA TGACCTCATC CACCCAGACT ACAGTGCTGT GACTGATGTA
    TACGTGCTGA TGTTTCTGGC TGACACTGTA GACTTCATTA TCATCGTCTT TGGATTCTGG
    GCCTTTGGGA AACACTCGGC AGCTGCAGAT ATCACCTCTT CTCTATCAGA AGACCAGGTC
    CCCGGGCCAT TCCTGGTGAT GGTCCTCATT CAGTTTGGAA CCATGGTGGT GGACCGTGCA
    CTCTACCTCA GGAAGACTGT GCTGGGCAAG GTCATCTTCC AGGTCATCCT CGTGTTTGGG
    ATTCACTTCT GGATGTTCTT CATCTTACCC GGTGTGACTG AGAGGAAATT CAGCCAGAAC
    CTGGTGGCAC AGCTTTGGTA CTTTGTGAAG TGTGTGTACT TTGGGCTGTC TGCCTATCAA
    ATCCGCTGTG GCTACCCAAC CCGTGTGCTC GGGAACTTCC TCACAAAGAG CTACAATTAC
    GTCAACCTCT TCTTGTTTCA AGGGTTCCGC CTGGTCCCAT TTCTGACTGA GCTGCGGGCT
    GTGATGGATT GGGTGTGGAC AGATACAACC CTGAGCCTCT CCAGCTGGAT TTGTGTGGAG
    GACATTTACG CGCACATTTT TATCTTAAAG TGCTGGCGAG AGTCGGAAAA GAGATACCCT
    CAACCCAGGG GTCAGAAGAA GAAGAAAGCC GTGAAGTACG GCATGGGCGG CATGATCATC
    GTCCTTCTCA TCTGTATCGT CTGGTTTCCT CTGCTCTTCA TGTCTTTGAT CAAATCTGTC
    GCAGGGGTTA TCAACCAACC CCTGGATGTG TCCGTCACAA TTACCCTGGG AGGTTATCAG
    CCTATCTTCA CAATGAGCGC CCAGCAAAGC CAGCTGAAAG TTATGGATAA TTCAAAGTAT
    AATGAATTTC TAAAATCTTT CGGCCCCAAC AGTGGCGCAA TGCAATTTCT GGAGAACTAT
    GAAAGGGAAG ACGTAACAGT AGCAGAACTG GAAGGGAACT CGAATTCTTT GTGGACCATC
    AGCCCTCCCA GTAAGCAGAA AATGATACAG GAGCTCACCG ACCCCAATAG TTGCTTCTCT
    GTTGTTTTTT CATGGAGTAT CCAGAGAAAC ATGACTTTGG GTGCAAAAGC CGAAATAGCA
    ACGGATAAGC TTTCTTTCCC TCTTGCGGTC GCAACACGGA ATAGCATAGC TAAAATGATT
    GCTGGCAATG ACACAGAAAG TTCAAACACG CCAGTGACAA TAGAGAAGAT CTACCCATAC
    TATGTGAAGG CACCCAGCGA TTCAAACTCA AAGCCTATAA AACAGCTTCT GTCTGAAAAT
    AATTTCATGA ATATCACCAT CATTTTGTTC AGAGACAATG TCACAAAGTC CAATAGTGAG
    TGGTGGGTTC TCAACCTGAC CGGAAGTAGG ATATTCAACC AGGGGTCCCA AGCCTTGGAA
    CTGGTGGTCT TCAATGACAA AGTCAGCCCT CCAAGTCTAG GCTTCTTGGC TGGCTATGGT
    ATCATGGGAT TGTATGCATC TGTTGTCCTT GTAATTGGGA AATTTGTTCG TGAGTTCTTC
    AGTGGGATCT CTCATTCCAT CATGTTTGAA GAGCTTCCAA ATGTGGACAG AATCTTGAAG
    TTGTGCACAG ATATATTCCT CGTGAGGGAG ACAGGGGAGT TGGAACTGGA GGAAGACCTC
    TACGCCAAGT TGATATTCCT GTACAGGTCA CCAGAAACCA TGATCAAGTG GACTAGAGAA
    AAAACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526229.1
    CDS1041..9572
    Translation

    Target ORF information:

    RefSeq Version XM_006526166.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526166.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGA GAGCTATTGG GAAGTTTGCC TCAGCCATTT TAGCCTTGCC AAAGTCTGTC
    ATTAAACTTC CCAAAACTAT TCTTCAGTAT TTAATCAGAG CTGCAAAGTT CGTCTACCAA
    GCCTGGATTA CTGATCCTAA AACGGCACTC CGACAGAGGA GGAAAGAGAA GAAAAAGTTG
    GCCAGAGAAG AGCAGAAGGA GAGACGCAAA GGATCTGGGG ACGGTCCAGT GGAATGGGAA
    GATCGAGAGG ATGAGCCAGT CAAAAAGAAA TCTGATGGAC CAGATAATAT CATCAAGAGG
    ATATTTAATA TCCTGAAGTT CACATGGGTT CTGTTCCTGG CCACAGTGGA TAGTTTCACC
    ACTTGGCTCA ACTCCATCTC AAGGGAACAC ATCGACATAT CCACAGTCCT GCGGATTGAA
    AGATGCATGC TGACTCGAGA GATAAAAAAG GGCAATGTTC CAACACGGGA AAGCATCCAC
    ATGTACTACC AGAACCACAT TATGAACCTT TCCCGAGAAT CCGGCCTGGA CACAATCGAC
    GAGCACTCGG GGGCTGGCTC CAGGGCCCAG GCAGCACATA GGATGGATAG CTTAGATTCC
    CGGGACAGTA TCTCCAGCTG CTACACTGAA GCGACCCTGC TGATCTCAAG GCAGTCCACC
    CTTGATGATT TAGATGGACA AGACCCCGTT CCTAAAACAA GCGAGCGTGC TCGGCCTAGG
    CTCCGTAAAA TGTTCAGCCT GGACATGTCC TCTTCGTCAG CTGACAGTGG CAGTGTAGCA
    TCAAGCGAGC CCACACAGTG TACCATGCTG TATTCGCGCC AAGGAACTAC GGAAACCATC
    GAGGAGGTGG AGGCTGAAGC TGAGGAGGAG GTGGTAGAAG GTCTGGAGCC TGAGCTCCAT
    GATGCAGAAG AAAAGGAATA CGCAGCTGAA TATGAAGCAG GGGTAGAGGA GATCAGCCTC
    ACTCCAGATG AGGAATTGCC TCAGTTCTCT ACAGATGACT GTGATGTGGA GGCGCCGCCC
    TCTTACAGCA AGGCTGTCAG CTTTGAGCAT CTATCGTTTG CGTCGCAAGA TGACTCTGGG
    GCCAAGAACC ACATGGTGGT CAGTCCTGAT GACAGCCGTA CAGACAAGCT GGAGTCAAGC
    ATCTTACCAC CTCTGACTCA TGAGCTGACA GCCAGCGATC TGCTGATGAG CAAGATGTTC
    CATGATGATG AGCTTGAGGA GTCTGAGAAG TTCTACGTGG ACCAGCCCCG GTTTTTGCTG
    CTATTCTATG CCATGTATAA CACCCTGGTG GCCCGCTCAG AAATGGTGTG CTATTTCGTG
    ATTATCCTGA ACCACATGAC CTCTGCCTCC ATCATCACTC TCCTGTTGCC CATCTTGATC
    TTCCTCTGGG CCATGCTGTC TGTTCCCAGG CCCAGCCGAC GCTTCTGGAT GATGGCCATC
    GTCTACACTG AGGTGGCGAT CGTAGTGAAA TACTTCTTCC AGTTCGGCTT CTTTCCCTGG
    AATAAGGACT TGGAAATATA CAAGGAAAGG CCCTACTTTC CACCGAACAT CATCGGGGTG
    GAGAAGAAGG AGGGCTATGT TCTTTATGAC CTCATTCAGC TGCTGGCTCT ATTCTTCCAT
    CGCTCCATTC TGAAGTGTCA TGGTCTATGG GACGAGGATG ACATTGTTGA CAGTAACACA
    GACAAGGAAG GATCGGATGA CGAGCTGTCC CTCGATCAGG GCAGAAGAGG CTCCTCAGAC
    TCACTGAAGT CTATCAACCT GGCTGCCTCT GTAGAGTCTG TCCACGTGAC CTTCCCCGAG
    CAGCCAGCGG CCATCAGGAG AAAACGCTCC TGCAGTAGCT CTCAGATCTC CCCACGGTCC
    AGCTTCTCTT CAAATAGGTC CAAAAGAGGC AGCACAAGCA CCCGGAACAG TAGCCAGAAA
    GGAAGCAGTG TTTTAAGCCT TAAACAGAAA AGCAAACGAG AACTTTACAT GGAAAAGCTT
    CAAGAGCATT TAATCAAAGC AAAGGCGTTT ACCATAAAGA AGACTCTACA GATCTATGTC
    CCCATTCGGC AATTCTTCTA TGACCTCATC CACCCAGACT ACAGTGCTGT GACTGATGTA
    TACGTGCTGA TGTTTCTGGC TGACACTGTA GACTTCATTA TCATCGTCTT TGGATTCTGG
    GCCTTTGGGA AACACTCGGC AGCTGCAGAT ATCACCTCTT CTCTATCAGA AGACCAGGTC
    CCCGGGCCAT TCCTGGTGAT GGTCCTCATT CAGTTTGGAA CCATGGTGGT GGACCGTGCA
    CTCTACCTCA GGAAGACTGT GCTGGGCAAG GTCATCTTCC AGGTCATCCT CGTGTTTGGG
    ATTCACTTCT GGATGTTCTT CATCTTACCC GGTGTGACTG AGAGGAAATT CAGCCAGAAC
    CTGGTGGCAC AGCTTTGGTA CTTTGTGAAG TGTGTGTACT TTGGGCTGTC TGCCTATCAA
    ATCCGCTGTG GCTACCCAAC CCGTGTGCTC GGGAACTTCC TCACAAAGAG CTACAATTAC
    GTCAACCTCT TCTTGTTTCA AGGGTTCCGC CTGGTCCCAT TTCTGACTGA GCTGCGGGCT
    GTGATGGATT GGGTGTGGAC AGATACAACC CTGAGCCTCT CCAGCTGGAT TTGTGTGGAG
    GACATTTACG CGCACATTTT TATCTTAAAG TGCTGGCGAG AGTCGGAAAA GAGATACCCT
    CAACCCAGGG GTCAGAAGAA GAAGAAAGCC GTGAAGTACG GCATGGGCGG CATGATCATC
    GTCCTTCTCA TCTGTATCGT CTGGTTTCCT CTGCTCTTCA TGTCTTTGAT CAAATCTGTC
    GCAGGGGTTA TCAACCAACC CCTGGATGTG TCCGTCACAA TTACCCTGGG AGGTTATCAG
    CCTATCTTCA CAATGAGCGC CCAGCAAAGC CAGCTGAAAG TTATGGATAA TTCAAAGTAT
    AATGAATTTC TAAAATCTTT CGGCCCCAAC AGTGGCGCAA TGCAATTTCT GGAGAACTAT
    GAAAGGGAAG ACGTAACAGT AGCAGAACTG GAAGGGAACT CGAATTCTTT GTGGACCATC
    AGCCCTCCCA GTAAGCAGAA AATGATACAG GAGCTCACCG ACCCCAATAG TTGCTTCTCT
    GTTGTTTTTT CATGGAGTAT CCAGAGAAAC ATGACTTTGG GTGCAAAAGC CGAAATAGCA
    ACGGATAAGC TTTCTTTCCC TCTTGCGGTC GCAACACGGA ATAGCATAGC TAAAATGATT
    GCTGGCAATG ACACAGAAAG TTCAAACACG CCAGTGACAA TAGAGAAGAT CTACCCATAC
    TATGTGAAGG CACCCAGCGA TTCAAACTCA AAGCCTATAA AACAGCTTCT GTCTGAAAAT
    AATTTCATGA ATATCACCAT CATTTTGTTC AGAGACAATG TCACAAAGTC CAATAGTGAG
    TGGTGGGTTC TCAACCTGAC CGGAAGTAGG ATATTCAACC AGGGGTCCCA AGCCTTGGAA
    CTGGTGGTCT TCAATGACAA AGTCAGCCCT CCAAGTCTAG GCTTCTTGGC TGGCTATGGT
    ATCATGGGAT TGTATGCATC TGTTGTCCTT GTAATTGGGA AATTTGTTCG TGAGTTCTTC
    AGTGGGATCT CTCATTCCAT CATGTTTGAA GAGCTTCCAA ATGTGGACAG AATCTTGAAG
    TTGTGCACAG ATATATTCCT CGTGAGGGAG ACAGGGGAGT TGGAACTGGA GGAAGACCTC
    TACGCCAAGT TGATATTCCT GTACAGGTCA CCAGAAACCA TGATCAAGTG GACTAGAGAA
    AAAACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54733
    Clone ID Related Accession (Same CDS sequence) XM_006526167.3 , XM_006526167.4
    Accession Version XM_006526167.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8529bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526167.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGATGAGGAG CTCCAAGATG TACAAGTGGA AGGGGAGCCC
    ACAGAGAAGG AAGAAGAAGA GGAAGAGGAA ATAAAGGAAG AAAGGCATGA GGTAAAGAAG
    GAAGAGGAAG AGGAAGTGGA GGAAGATGAT GACCAGGACA TCATGAAGGT GCTGGGTAAT
    CTGGTGGTGG CCCTCTTCAT CAAGTATTGG ATCTACGTCT GCGGGGGCAT GTTCTTTTTT
    GTCAGCTTTG AGGGGAAAAT CGTCATGTAC AAAATCATCT ACATGGTGCT GTTCCTGTTC
    TGCGTGGCCT TGTACCAGGT GCACTACGAG TGGTGGAGGA AGATCCTGAA GTACTTCTGG
    ATGTCGGTGG TCATTTATAC CATGTTGGTG CTCATCTTCA TATACACTTA CCAGTTTGAA
    AACTTCCCAG GCCTGTGGCA AAACATGACC GGATTAAAGA AAGAAAAGCT CGAGGACCTC
    GGCCTGAAGC AGTTCACGGT CGCTGAACTG TTCACTCGCA TATTCATCCC CACCTCCTTT
    CTACTCGTCT GCATCCTACA CCTCCACTAC TTCCACGATC GGTTCCTGGA ACTTACAGAC
    CTCAAGTCCA TCCCCAGCAA AGAAGACAAC ACCATCTACA GGTTGGCCCA CCCTGAAGGA
    AGTCTTCCAG ACTTAGCCAT CATGAATATG ACGGCCAGCC TTGATAAGCC TGAGGTGCAG
    AAGTTGGCAG AGTCTGGGGA GGAGAGGCCA GAAGAGTGTG TCAAGAAGAC GGAGAAGGGT
    GAGGCTGGGA AAGACAGTGA TGAGTCTGAG GAGGAGGAAG ATGAAGAAGA GGAATCCGAG
    GAAGAAGAAA GCTCAGACCT CAGGAACAAG TGGCACCTGG TGATTGACCG GCTCACTGTG
    CTCTTTCTAA AATTCCTGGA GTATTTCCAC AAGTTGCAGG TGTTTATGTG GTGGATCTTG
    GAGCTGCACA TCATCAAAAT CGTCTCATCG TACATTATCT GGGTGACTGT GAAAGAGGTG
    TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCATTTG CTCTGCCATA TGCCAAACTC
    CGTCGTGCGG CTTCCAGTGT CTGCACTGTC TGGACATGTG TGATCATCGT CTGCAAAATG
    TTGTACCAGC TGCAAACCAT TAAACCTGAG AACTTTTCTG TCAACTGCTC CTTGCCCAAT
    GAAAATCAAA CCAACATTCC CCTTCATGAG TTGAACAAGT CTCTTCTCTA CAGTGCCCCT
    GTTGATCCCA CTGAGTGGGT AGGCCTGAGG AAGTCCTCTC CTTTGCTCGT CTACCTGAGG
    AACAACCTCC TGATGCTGGC GATTTTGGCC TTTGAAGTCA CCGTTTACCG CCATCAGGAG
    TACTATCGAG GCCGAAACAA CCTGACTGCT CCAGTGTCTA AAACTATCTT TCATGACATC
    ACAAGGTTAC ATCTAGATGA TGGGCTTATC AATTGTGCCA AATATTTTGT AAATTACTTC
    TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCCG TGAATGTAAT TGGACAGCGA
    ATGGACTTCT ACGCCATGAT TCATGCCTGT TGGTTGATCG GTGTCTTGTA TCGACGCAGA
    AGAAAAGCCA TTGCCGAGGT CTGGCCCAAG TACTGCTGCT TCCTGGCTTG CATCATCACC
    TTCCAGTACT TCGTCTGCAT CGGCATCCCC CCTGCCCCTT GCCGAGATTA TCCATGGAGA
    TTTAAGGGAG CCTACTTCAA TGATAACATT ATCAAGTGGC TGTACTTCCC GGATTTCATT
    GTGCGGCCCA ACCCCGTGTT TCTTGTCTAT GACTTCATGC TGCTCCTGTG TGCTTCCTTA
    CAACGACAGA TCTTTGAGGA CGAAAACAAG GCAGCCGTGC GCATCATGGC CGGGGACAAT
    GTCGAGATCT GCATGAACCT TGATGCAGCC TCGTTCAGCC AGCATAACCC TGTGCCAGAT
    TTCATTCACT GCAGATCTTA CTTGGACATG TCCAAAGTGA TCATCTTCAG CTACCTCTTC
    TGGTTCGTCC TCACCATCAT CTTCATTACG GGAACAACCA GGATCAGCAT ATTTTGCATG
    GGCTACTTGG TGGCCTGTTT CTATTTCCTG CTCTTTGGAG GGGATCTGCT GCTGAAGCCC
    ATCAAGAGCA TCCTTCGTTA CTGGGACTGG CTGATTGCAT ACAACGTGTT TGTGATCACC
    ATGAAGAATA TACTGTCAAT AGGAGCCTGT GGATACATTG GCGCACTAGT AAGAAACAGC
    TGCTGGTTGA TCCAAGCGTT CAGCCTGGCC TGCACCGTGA AAGGCTATCA GATGCCTGAA
    GATGACTCCA GATGCAAGCT GCCCAGTGGG GAGGCAGGGA TCATCTGGGA CAGCATCTGC
    TTTGCGTTTC TGCTGCTGCA GAGGAGAGTC TTCATGAGCT ACTACTTCCT GCATGTCGTG
    GCTGACATTA AAGCATCCCA GATCCTGGCA TCCAGAGGGG CCGAACTTTT CCAGGCTACA
    ATTGTGAAAG CTGTAAAGGC AAGAATTGAA GAAGAGAAAA AATCCATGGA CCAGCTGAAG
    AGACAGATGG ATCGCATCAA AGCCCGGCAA CAAAAGTACA AAAAGGGTAA GGAGAGGATG
    CTGAGCTTGA CCCAGGAGTC AGGGGAAGGC CAGGACATCC AGAAAGTCTC AGAAGAAGAT
    GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAGGGCAAAA AAAAGCAGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTTAAAAAG GAGGATGAAG AACCCCCACG GAAGTCAGCA
    TTCCAGAGAG CTATTGGGAA GTTTGCCTCA GCCATTTTAG CCTTGCCAAA GTCTGTCATT
    AAACTTCCCA AAACTATTCT TCAGTATTTA ATCAGAGCTG CAAAGTTCGT CTACCAAGCC
    TGGATTACTG ATCCTAAAAC GGCACTCCGA CAGAGGAGGA AAGAGAAGAA AAAGTTGGCC
    AGAGAAGAGC AGAAGGAGAG ACGCAAAGGA TCTGGGGACG GTCCAGTGGA ATGGGAAGAT
    CGAGAGGATG AGCCAGTCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC ATGGGTTCTG TTCCTGGCCA CAGTGGATAG TTTCACCACT
    TGGCTCAACT CCATCTCAAG GGAACACATC GACATATCCA CAGTCCTGCG GATTGAAAGA
    TGCATGCTGA CTCGAGAGAT AAAAAAGGGC AATGTTCCAA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATTAT GAACCTTTCC CGAGAATCCG GCCTGGACAC AATCGACGAG
    CACTCGGGGG CTGGCTCCAG GGCCCAGGCA GCACATAGGA TGGATAGCTT AGATTCCCGG
    GACAGTATCT CCAGCTGCTA CACTGAAGCG ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCCGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAATGT TCAGCCTGGA CATGTCCTCT TCGTCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGTAC CATGCTGTAT TCGCGCCAAG GAACTACGGA AACCATCGAG
    GAGGTGGAGG CTGAAGCTGA GGAGGAGGTG GTAGAAGGTC TGGAGCCTGA GCTCCATGAT
    GCAGAAGAAA AGGAATACGC AGCTGAATAT GAAGCAGGGG TAGAGGAGAT CAGCCTCACT
    CCAGATGAGG AATTGCCTCA GTTCTCTACA GATGACTGTG ATGTGGAGGC GCCGCCCTCT
    TACAGCAAGG CTGTCAGCTT TGAGCATCTA TCGTTTGCGT CGCAAGATGA CTCTGGGGCC
    AAGAACCACA TGGTGGTCAG TCCTGATGAC AGCCGTACAG ACAAGCTGGA GTCAAGCATC
    TTACCACCTC TGACTCATGA GCTGACAGCC AGCGATCTGC TGATGAGCAA GATGTTCCAT
    GATGATGAGC TTGAGGAGTC TGAGAAGTTC TACGTGGACC AGCCCCGGTT TTTGCTGCTA
    TTCTATGCCA TGTATAACAC CCTGGTGGCC CGCTCAGAAA TGGTGTGCTA TTTCGTGATT
    ATCCTGAACC ACATGACCTC TGCCTCCATC ATCACTCTCC TGTTGCCCAT CTTGATCTTC
    CTCTGGGCCA TGCTGTCTGT TCCCAGGCCC AGCCGACGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATCGT AGTGAAATAC TTCTTCCAGT TCGGCTTCTT TCCCTGGAAT
    AAGGACTTGG AAATATACAA GGAAAGGCCC TACTTTCCAC CGAACATCAT CGGGGTGGAG
    AAGAAGGAGG GCTATGTTCT TTATGACCTC ATTCAGCTGC TGGCTCTATT CTTCCATCGC
    TCCATTCTGA AGTGTCATGG TCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CGGATGACGA GCTGTCCCTC GATCAGGGCA GAAGAGGCTC CTCAGACTCA
    CTGAAGTCTA TCAACCTGGC TGCCTCTGTA GAGTCTGTCC ACGTGACCTT CCCCGAGCAG
    CCAGCGGCCA TCAGGAGAAA ACGCTCCTGC AGTAGCTCTC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GGAACAGTAG CCAGAAAGGA
    AGCAGTGTTT TAAGCCTTAA ACAGAAAAGC AAACGAGAAC TTTACATGGA AAAGCTTCAA
    GAGCATTTAA TCAAAGCAAA GGCGTTTACC ATAAAGAAGA CTCTACAGAT CTATGTCCCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGATGTATAC
    GTGCTGATGT TTCTGGCTGA CACTGTAGAC TTCATTATCA TCGTCTTTGG ATTCTGGGCC
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCTC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT CCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT GGGCAAGGTC ATCTTCCAGG TCATCCTCGT GTTTGGGATT
    CACTTCTGGA TGTTCTTCAT CTTACCCGGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TTTGGTACTT TGTGAAGTGT GTGTACTTTG GGCTGTCTGC CTATCAAATC
    CGCTGTGGCT ACCCAACCCG TGTGCTCGGG AACTTCCTCA CAAAGAGCTA CAATTACGTC
    AACCTCTTCT TGTTTCAAGG GTTCCGCCTG GTCCCATTTC TGACTGAGCT GCGGGCTGTG
    ATGGATTGGG TGTGGACAGA TACAACCCTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCGC ACATTTTTAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGTC AGAAGAAGAA GAAAGCCGTG AAGTACGGCA TGGGCGGCAT GATCATCGTC
    CTTCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA ATCTGTCGCA
    GGGGTTATCA ACCAACCCCT GGATGTGTCC GTCACAATTA CCCTGGGAGG TTATCAGCCT
    ATCTTCACAA TGAGCGCCCA GCAAAGCCAG CTGAAAGTTA TGGATAATTC AAAGTATAAT
    GAATTTCTAA AATCTTTCGG CCCCAACAGT GGCGCAATGC AATTTCTGGA GAACTATGAA
    AGGGAAGACG TAACAGTAGC AGAACTGGAA GGGAACTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTTTGGGTG CAAAAGCCGA AATAGCAACG
    GATAAGCTTT CTTTCCCTCT TGCGGTCGCA ACACGGAATA GCATAGCTAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC AAACACGCCA GTGACAATAG AGAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGCGATTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC TGAAAATAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACCGG AAGTAGGATA TTCAACCAGG GGTCCCAAGC CTTGGAACTG
    GTGGTCTTCA ATGACAAAGT CAGCCCTCCA AGTCTAGGCT TCTTGGCTGG CTATGGTATC
    ATGGGATTGT ATGCATCTGT TGTCCTTGTA ATTGGGAAAT TTGTTCGTGA GTTCTTCAGT
    GGGATCTCTC ATTCCATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT CTTGAAGTTG
    TGCACAGATA TATTCCTCGT GAGGGAGACA GGGGAGTTGG AACTGGAGGA AGACCTCTAC
    GCCAAGTTGA TATTCCTGTA CAGGTCACCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526230.1
    CDS1041..9569
    Translation

    Target ORF information:

    RefSeq Version XM_006526167.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526167.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGATGAGGAG CTCCAAGATG TACAAGTGGA AGGGGAGCCC
    ACAGAGAAGG AAGAAGAAGA GGAAGAGGAA ATAAAGGAAG AAAGGCATGA GGTAAAGAAG
    GAAGAGGAAG AGGAAGTGGA GGAAGATGAT GACCAGGACA TCATGAAGGT GCTGGGTAAT
    CTGGTGGTGG CCCTCTTCAT CAAGTATTGG ATCTACGTCT GCGGGGGCAT GTTCTTTTTT
    GTCAGCTTTG AGGGGAAAAT CGTCATGTAC AAAATCATCT ACATGGTGCT GTTCCTGTTC
    TGCGTGGCCT TGTACCAGGT GCACTACGAG TGGTGGAGGA AGATCCTGAA GTACTTCTGG
    ATGTCGGTGG TCATTTATAC CATGTTGGTG CTCATCTTCA TATACACTTA CCAGTTTGAA
    AACTTCCCAG GCCTGTGGCA AAACATGACC GGATTAAAGA AAGAAAAGCT CGAGGACCTC
    GGCCTGAAGC AGTTCACGGT CGCTGAACTG TTCACTCGCA TATTCATCCC CACCTCCTTT
    CTACTCGTCT GCATCCTACA CCTCCACTAC TTCCACGATC GGTTCCTGGA ACTTACAGAC
    CTCAAGTCCA TCCCCAGCAA AGAAGACAAC ACCATCTACA GGTTGGCCCA CCCTGAAGGA
    AGTCTTCCAG ACTTAGCCAT CATGAATATG ACGGCCAGCC TTGATAAGCC TGAGGTGCAG
    AAGTTGGCAG AGTCTGGGGA GGAGAGGCCA GAAGAGTGTG TCAAGAAGAC GGAGAAGGGT
    GAGGCTGGGA AAGACAGTGA TGAGTCTGAG GAGGAGGAAG ATGAAGAAGA GGAATCCGAG
    GAAGAAGAAA GCTCAGACCT CAGGAACAAG TGGCACCTGG TGATTGACCG GCTCACTGTG
    CTCTTTCTAA AATTCCTGGA GTATTTCCAC AAGTTGCAGG TGTTTATGTG GTGGATCTTG
    GAGCTGCACA TCATCAAAAT CGTCTCATCG TACATTATCT GGGTGACTGT GAAAGAGGTG
    TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCATTTG CTCTGCCATA TGCCAAACTC
    CGTCGTGCGG CTTCCAGTGT CTGCACTGTC TGGACATGTG TGATCATCGT CTGCAAAATG
    TTGTACCAGC TGCAAACCAT TAAACCTGAG AACTTTTCTG TCAACTGCTC CTTGCCCAAT
    GAAAATCAAA CCAACATTCC CCTTCATGAG TTGAACAAGT CTCTTCTCTA CAGTGCCCCT
    GTTGATCCCA CTGAGTGGGT AGGCCTGAGG AAGTCCTCTC CTTTGCTCGT CTACCTGAGG
    AACAACCTCC TGATGCTGGC GATTTTGGCC TTTGAAGTCA CCGTTTACCG CCATCAGGAG
    TACTATCGAG GCCGAAACAA CCTGACTGCT CCAGTGTCTA AAACTATCTT TCATGACATC
    ACAAGGTTAC ATCTAGATGA TGGGCTTATC AATTGTGCCA AATATTTTGT AAATTACTTC
    TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCCG TGAATGTAAT TGGACAGCGA
    ATGGACTTCT ACGCCATGAT TCATGCCTGT TGGTTGATCG GTGTCTTGTA TCGACGCAGA
    AGAAAAGCCA TTGCCGAGGT CTGGCCCAAG TACTGCTGCT TCCTGGCTTG CATCATCACC
    TTCCAGTACT TCGTCTGCAT CGGCATCCCC CCTGCCCCTT GCCGAGATTA TCCATGGAGA
    TTTAAGGGAG CCTACTTCAA TGATAACATT ATCAAGTGGC TGTACTTCCC GGATTTCATT
    GTGCGGCCCA ACCCCGTGTT TCTTGTCTAT GACTTCATGC TGCTCCTGTG TGCTTCCTTA
    CAACGACAGA TCTTTGAGGA CGAAAACAAG GCAGCCGTGC GCATCATGGC CGGGGACAAT
    GTCGAGATCT GCATGAACCT TGATGCAGCC TCGTTCAGCC AGCATAACCC TGTGCCAGAT
    TTCATTCACT GCAGATCTTA CTTGGACATG TCCAAAGTGA TCATCTTCAG CTACCTCTTC
    TGGTTCGTCC TCACCATCAT CTTCATTACG GGAACAACCA GGATCAGCAT ATTTTGCATG
    GGCTACTTGG TGGCCTGTTT CTATTTCCTG CTCTTTGGAG GGGATCTGCT GCTGAAGCCC
    ATCAAGAGCA TCCTTCGTTA CTGGGACTGG CTGATTGCAT ACAACGTGTT TGTGATCACC
    ATGAAGAATA TACTGTCAAT AGGAGCCTGT GGATACATTG GCGCACTAGT AAGAAACAGC
    TGCTGGTTGA TCCAAGCGTT CAGCCTGGCC TGCACCGTGA AAGGCTATCA GATGCCTGAA
    GATGACTCCA GATGCAAGCT GCCCAGTGGG GAGGCAGGGA TCATCTGGGA CAGCATCTGC
    TTTGCGTTTC TGCTGCTGCA GAGGAGAGTC TTCATGAGCT ACTACTTCCT GCATGTCGTG
    GCTGACATTA AAGCATCCCA GATCCTGGCA TCCAGAGGGG CCGAACTTTT CCAGGCTACA
    ATTGTGAAAG CTGTAAAGGC AAGAATTGAA GAAGAGAAAA AATCCATGGA CCAGCTGAAG
    AGACAGATGG ATCGCATCAA AGCCCGGCAA CAAAAGTACA AAAAGGGTAA GGAGAGGATG
    CTGAGCTTGA CCCAGGAGTC AGGGGAAGGC CAGGACATCC AGAAAGTCTC AGAAGAAGAT
    GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAGGGCAAAA AAAAGCAGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTTAAAAAG GAGGATGAAG AACCCCCACG GAAGTCAGCA
    TTCCAGAGAG CTATTGGGAA GTTTGCCTCA GCCATTTTAG CCTTGCCAAA GTCTGTCATT
    AAACTTCCCA AAACTATTCT TCAGTATTTA ATCAGAGCTG CAAAGTTCGT CTACCAAGCC
    TGGATTACTG ATCCTAAAAC GGCACTCCGA CAGAGGAGGA AAGAGAAGAA AAAGTTGGCC
    AGAGAAGAGC AGAAGGAGAG ACGCAAAGGA TCTGGGGACG GTCCAGTGGA ATGGGAAGAT
    CGAGAGGATG AGCCAGTCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC ATGGGTTCTG TTCCTGGCCA CAGTGGATAG TTTCACCACT
    TGGCTCAACT CCATCTCAAG GGAACACATC GACATATCCA CAGTCCTGCG GATTGAAAGA
    TGCATGCTGA CTCGAGAGAT AAAAAAGGGC AATGTTCCAA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATTAT GAACCTTTCC CGAGAATCCG GCCTGGACAC AATCGACGAG
    CACTCGGGGG CTGGCTCCAG GGCCCAGGCA GCACATAGGA TGGATAGCTT AGATTCCCGG
    GACAGTATCT CCAGCTGCTA CACTGAAGCG ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCCGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAATGT TCAGCCTGGA CATGTCCTCT TCGTCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGTAC CATGCTGTAT TCGCGCCAAG GAACTACGGA AACCATCGAG
    GAGGTGGAGG CTGAAGCTGA GGAGGAGGTG GTAGAAGGTC TGGAGCCTGA GCTCCATGAT
    GCAGAAGAAA AGGAATACGC AGCTGAATAT GAAGCAGGGG TAGAGGAGAT CAGCCTCACT
    CCAGATGAGG AATTGCCTCA GTTCTCTACA GATGACTGTG ATGTGGAGGC GCCGCCCTCT
    TACAGCAAGG CTGTCAGCTT TGAGCATCTA TCGTTTGCGT CGCAAGATGA CTCTGGGGCC
    AAGAACCACA TGGTGGTCAG TCCTGATGAC AGCCGTACAG ACAAGCTGGA GTCAAGCATC
    TTACCACCTC TGACTCATGA GCTGACAGCC AGCGATCTGC TGATGAGCAA GATGTTCCAT
    GATGATGAGC TTGAGGAGTC TGAGAAGTTC TACGTGGACC AGCCCCGGTT TTTGCTGCTA
    TTCTATGCCA TGTATAACAC CCTGGTGGCC CGCTCAGAAA TGGTGTGCTA TTTCGTGATT
    ATCCTGAACC ACATGACCTC TGCCTCCATC ATCACTCTCC TGTTGCCCAT CTTGATCTTC
    CTCTGGGCCA TGCTGTCTGT TCCCAGGCCC AGCCGACGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATCGT AGTGAAATAC TTCTTCCAGT TCGGCTTCTT TCCCTGGAAT
    AAGGACTTGG AAATATACAA GGAAAGGCCC TACTTTCCAC CGAACATCAT CGGGGTGGAG
    AAGAAGGAGG GCTATGTTCT TTATGACCTC ATTCAGCTGC TGGCTCTATT CTTCCATCGC
    TCCATTCTGA AGTGTCATGG TCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CGGATGACGA GCTGTCCCTC GATCAGGGCA GAAGAGGCTC CTCAGACTCA
    CTGAAGTCTA TCAACCTGGC TGCCTCTGTA GAGTCTGTCC ACGTGACCTT CCCCGAGCAG
    CCAGCGGCCA TCAGGAGAAA ACGCTCCTGC AGTAGCTCTC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GGAACAGTAG CCAGAAAGGA
    AGCAGTGTTT TAAGCCTTAA ACAGAAAAGC AAACGAGAAC TTTACATGGA AAAGCTTCAA
    GAGCATTTAA TCAAAGCAAA GGCGTTTACC ATAAAGAAGA CTCTACAGAT CTATGTCCCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGATGTATAC
    GTGCTGATGT TTCTGGCTGA CACTGTAGAC TTCATTATCA TCGTCTTTGG ATTCTGGGCC
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCTC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT CCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT GGGCAAGGTC ATCTTCCAGG TCATCCTCGT GTTTGGGATT
    CACTTCTGGA TGTTCTTCAT CTTACCCGGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TTTGGTACTT TGTGAAGTGT GTGTACTTTG GGCTGTCTGC CTATCAAATC
    CGCTGTGGCT ACCCAACCCG TGTGCTCGGG AACTTCCTCA CAAAGAGCTA CAATTACGTC
    AACCTCTTCT TGTTTCAAGG GTTCCGCCTG GTCCCATTTC TGACTGAGCT GCGGGCTGTG
    ATGGATTGGG TGTGGACAGA TACAACCCTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCGC ACATTTTTAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGTC AGAAGAAGAA GAAAGCCGTG AAGTACGGCA TGGGCGGCAT GATCATCGTC
    CTTCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA ATCTGTCGCA
    GGGGTTATCA ACCAACCCCT GGATGTGTCC GTCACAATTA CCCTGGGAGG TTATCAGCCT
    ATCTTCACAA TGAGCGCCCA GCAAAGCCAG CTGAAAGTTA TGGATAATTC AAAGTATAAT
    GAATTTCTAA AATCTTTCGG CCCCAACAGT GGCGCAATGC AATTTCTGGA GAACTATGAA
    AGGGAAGACG TAACAGTAGC AGAACTGGAA GGGAACTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTTTGGGTG CAAAAGCCGA AATAGCAACG
    GATAAGCTTT CTTTCCCTCT TGCGGTCGCA ACACGGAATA GCATAGCTAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC AAACACGCCA GTGACAATAG AGAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGCGATTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC TGAAAATAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACCGG AAGTAGGATA TTCAACCAGG GGTCCCAAGC CTTGGAACTG
    GTGGTCTTCA ATGACAAAGT CAGCCCTCCA AGTCTAGGCT TCTTGGCTGG CTATGGTATC
    ATGGGATTGT ATGCATCTGT TGTCCTTGTA ATTGGGAAAT TTGTTCGTGA GTTCTTCAGT
    GGGATCTCTC ATTCCATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT CTTGAAGTTG
    TGCACAGATA TATTCCTCGT GAGGGAGACA GGGGAGTTGG AACTGGAGGA AGACCTCTAC
    GCCAAGTTGA TATTCCTGTA CAGGTCACCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54733
    Clone ID Related Accession (Same CDS sequence) XM_006526167.3 , XM_006526167.4
    Accession Version XM_006526167.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8529bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526167.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGATGAGGAG CTCCAAGATG TACAAGTGGA AGGGGAGCCC
    ACAGAGAAGG AAGAAGAAGA GGAAGAGGAA ATAAAGGAAG AAAGGCATGA GGTAAAGAAG
    GAAGAGGAAG AGGAAGTGGA GGAAGATGAT GACCAGGACA TCATGAAGGT GCTGGGTAAT
    CTGGTGGTGG CCCTCTTCAT CAAGTATTGG ATCTACGTCT GCGGGGGCAT GTTCTTTTTT
    GTCAGCTTTG AGGGGAAAAT CGTCATGTAC AAAATCATCT ACATGGTGCT GTTCCTGTTC
    TGCGTGGCCT TGTACCAGGT GCACTACGAG TGGTGGAGGA AGATCCTGAA GTACTTCTGG
    ATGTCGGTGG TCATTTATAC CATGTTGGTG CTCATCTTCA TATACACTTA CCAGTTTGAA
    AACTTCCCAG GCCTGTGGCA AAACATGACC GGATTAAAGA AAGAAAAGCT CGAGGACCTC
    GGCCTGAAGC AGTTCACGGT CGCTGAACTG TTCACTCGCA TATTCATCCC CACCTCCTTT
    CTACTCGTCT GCATCCTACA CCTCCACTAC TTCCACGATC GGTTCCTGGA ACTTACAGAC
    CTCAAGTCCA TCCCCAGCAA AGAAGACAAC ACCATCTACA GGTTGGCCCA CCCTGAAGGA
    AGTCTTCCAG ACTTAGCCAT CATGAATATG ACGGCCAGCC TTGATAAGCC TGAGGTGCAG
    AAGTTGGCAG AGTCTGGGGA GGAGAGGCCA GAAGAGTGTG TCAAGAAGAC GGAGAAGGGT
    GAGGCTGGGA AAGACAGTGA TGAGTCTGAG GAGGAGGAAG ATGAAGAAGA GGAATCCGAG
    GAAGAAGAAA GCTCAGACCT CAGGAACAAG TGGCACCTGG TGATTGACCG GCTCACTGTG
    CTCTTTCTAA AATTCCTGGA GTATTTCCAC AAGTTGCAGG TGTTTATGTG GTGGATCTTG
    GAGCTGCACA TCATCAAAAT CGTCTCATCG TACATTATCT GGGTGACTGT GAAAGAGGTG
    TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCATTTG CTCTGCCATA TGCCAAACTC
    CGTCGTGCGG CTTCCAGTGT CTGCACTGTC TGGACATGTG TGATCATCGT CTGCAAAATG
    TTGTACCAGC TGCAAACCAT TAAACCTGAG AACTTTTCTG TCAACTGCTC CTTGCCCAAT
    GAAAATCAAA CCAACATTCC CCTTCATGAG TTGAACAAGT CTCTTCTCTA CAGTGCCCCT
    GTTGATCCCA CTGAGTGGGT AGGCCTGAGG AAGTCCTCTC CTTTGCTCGT CTACCTGAGG
    AACAACCTCC TGATGCTGGC GATTTTGGCC TTTGAAGTCA CCGTTTACCG CCATCAGGAG
    TACTATCGAG GCCGAAACAA CCTGACTGCT CCAGTGTCTA AAACTATCTT TCATGACATC
    ACAAGGTTAC ATCTAGATGA TGGGCTTATC AATTGTGCCA AATATTTTGT AAATTACTTC
    TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCCG TGAATGTAAT TGGACAGCGA
    ATGGACTTCT ACGCCATGAT TCATGCCTGT TGGTTGATCG GTGTCTTGTA TCGACGCAGA
    AGAAAAGCCA TTGCCGAGGT CTGGCCCAAG TACTGCTGCT TCCTGGCTTG CATCATCACC
    TTCCAGTACT TCGTCTGCAT CGGCATCCCC CCTGCCCCTT GCCGAGATTA TCCATGGAGA
    TTTAAGGGAG CCTACTTCAA TGATAACATT ATCAAGTGGC TGTACTTCCC GGATTTCATT
    GTGCGGCCCA ACCCCGTGTT TCTTGTCTAT GACTTCATGC TGCTCCTGTG TGCTTCCTTA
    CAACGACAGA TCTTTGAGGA CGAAAACAAG GCAGCCGTGC GCATCATGGC CGGGGACAAT
    GTCGAGATCT GCATGAACCT TGATGCAGCC TCGTTCAGCC AGCATAACCC TGTGCCAGAT
    TTCATTCACT GCAGATCTTA CTTGGACATG TCCAAAGTGA TCATCTTCAG CTACCTCTTC
    TGGTTCGTCC TCACCATCAT CTTCATTACG GGAACAACCA GGATCAGCAT ATTTTGCATG
    GGCTACTTGG TGGCCTGTTT CTATTTCCTG CTCTTTGGAG GGGATCTGCT GCTGAAGCCC
    ATCAAGAGCA TCCTTCGTTA CTGGGACTGG CTGATTGCAT ACAACGTGTT TGTGATCACC
    ATGAAGAATA TACTGTCAAT AGGAGCCTGT GGATACATTG GCGCACTAGT AAGAAACAGC
    TGCTGGTTGA TCCAAGCGTT CAGCCTGGCC TGCACCGTGA AAGGCTATCA GATGCCTGAA
    GATGACTCCA GATGCAAGCT GCCCAGTGGG GAGGCAGGGA TCATCTGGGA CAGCATCTGC
    TTTGCGTTTC TGCTGCTGCA GAGGAGAGTC TTCATGAGCT ACTACTTCCT GCATGTCGTG
    GCTGACATTA AAGCATCCCA GATCCTGGCA TCCAGAGGGG CCGAACTTTT CCAGGCTACA
    ATTGTGAAAG CTGTAAAGGC AAGAATTGAA GAAGAGAAAA AATCCATGGA CCAGCTGAAG
    AGACAGATGG ATCGCATCAA AGCCCGGCAA CAAAAGTACA AAAAGGGTAA GGAGAGGATG
    CTGAGCTTGA CCCAGGAGTC AGGGGAAGGC CAGGACATCC AGAAAGTCTC AGAAGAAGAT
    GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAGGGCAAAA AAAAGCAGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTTAAAAAG GAGGATGAAG AACCCCCACG GAAGTCAGCA
    TTCCAGAGAG CTATTGGGAA GTTTGCCTCA GCCATTTTAG CCTTGCCAAA GTCTGTCATT
    AAACTTCCCA AAACTATTCT TCAGTATTTA ATCAGAGCTG CAAAGTTCGT CTACCAAGCC
    TGGATTACTG ATCCTAAAAC GGCACTCCGA CAGAGGAGGA AAGAGAAGAA AAAGTTGGCC
    AGAGAAGAGC AGAAGGAGAG ACGCAAAGGA TCTGGGGACG GTCCAGTGGA ATGGGAAGAT
    CGAGAGGATG AGCCAGTCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC ATGGGTTCTG TTCCTGGCCA CAGTGGATAG TTTCACCACT
    TGGCTCAACT CCATCTCAAG GGAACACATC GACATATCCA CAGTCCTGCG GATTGAAAGA
    TGCATGCTGA CTCGAGAGAT AAAAAAGGGC AATGTTCCAA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATTAT GAACCTTTCC CGAGAATCCG GCCTGGACAC AATCGACGAG
    CACTCGGGGG CTGGCTCCAG GGCCCAGGCA GCACATAGGA TGGATAGCTT AGATTCCCGG
    GACAGTATCT CCAGCTGCTA CACTGAAGCG ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCCGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAATGT TCAGCCTGGA CATGTCCTCT TCGTCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGTAC CATGCTGTAT TCGCGCCAAG GAACTACGGA AACCATCGAG
    GAGGTGGAGG CTGAAGCTGA GGAGGAGGTG GTAGAAGGTC TGGAGCCTGA GCTCCATGAT
    GCAGAAGAAA AGGAATACGC AGCTGAATAT GAAGCAGGGG TAGAGGAGAT CAGCCTCACT
    CCAGATGAGG AATTGCCTCA GTTCTCTACA GATGACTGTG ATGTGGAGGC GCCGCCCTCT
    TACAGCAAGG CTGTCAGCTT TGAGCATCTA TCGTTTGCGT CGCAAGATGA CTCTGGGGCC
    AAGAACCACA TGGTGGTCAG TCCTGATGAC AGCCGTACAG ACAAGCTGGA GTCAAGCATC
    TTACCACCTC TGACTCATGA GCTGACAGCC AGCGATCTGC TGATGAGCAA GATGTTCCAT
    GATGATGAGC TTGAGGAGTC TGAGAAGTTC TACGTGGACC AGCCCCGGTT TTTGCTGCTA
    TTCTATGCCA TGTATAACAC CCTGGTGGCC CGCTCAGAAA TGGTGTGCTA TTTCGTGATT
    ATCCTGAACC ACATGACCTC TGCCTCCATC ATCACTCTCC TGTTGCCCAT CTTGATCTTC
    CTCTGGGCCA TGCTGTCTGT TCCCAGGCCC AGCCGACGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATCGT AGTGAAATAC TTCTTCCAGT TCGGCTTCTT TCCCTGGAAT
    AAGGACTTGG AAATATACAA GGAAAGGCCC TACTTTCCAC CGAACATCAT CGGGGTGGAG
    AAGAAGGAGG GCTATGTTCT TTATGACCTC ATTCAGCTGC TGGCTCTATT CTTCCATCGC
    TCCATTCTGA AGTGTCATGG TCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CGGATGACGA GCTGTCCCTC GATCAGGGCA GAAGAGGCTC CTCAGACTCA
    CTGAAGTCTA TCAACCTGGC TGCCTCTGTA GAGTCTGTCC ACGTGACCTT CCCCGAGCAG
    CCAGCGGCCA TCAGGAGAAA ACGCTCCTGC AGTAGCTCTC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GGAACAGTAG CCAGAAAGGA
    AGCAGTGTTT TAAGCCTTAA ACAGAAAAGC AAACGAGAAC TTTACATGGA AAAGCTTCAA
    GAGCATTTAA TCAAAGCAAA GGCGTTTACC ATAAAGAAGA CTCTACAGAT CTATGTCCCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGATGTATAC
    GTGCTGATGT TTCTGGCTGA CACTGTAGAC TTCATTATCA TCGTCTTTGG ATTCTGGGCC
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCTC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT CCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT GGGCAAGGTC ATCTTCCAGG TCATCCTCGT GTTTGGGATT
    CACTTCTGGA TGTTCTTCAT CTTACCCGGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TTTGGTACTT TGTGAAGTGT GTGTACTTTG GGCTGTCTGC CTATCAAATC
    CGCTGTGGCT ACCCAACCCG TGTGCTCGGG AACTTCCTCA CAAAGAGCTA CAATTACGTC
    AACCTCTTCT TGTTTCAAGG GTTCCGCCTG GTCCCATTTC TGACTGAGCT GCGGGCTGTG
    ATGGATTGGG TGTGGACAGA TACAACCCTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCGC ACATTTTTAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGTC AGAAGAAGAA GAAAGCCGTG AAGTACGGCA TGGGCGGCAT GATCATCGTC
    CTTCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA ATCTGTCGCA
    GGGGTTATCA ACCAACCCCT GGATGTGTCC GTCACAATTA CCCTGGGAGG TTATCAGCCT
    ATCTTCACAA TGAGCGCCCA GCAAAGCCAG CTGAAAGTTA TGGATAATTC AAAGTATAAT
    GAATTTCTAA AATCTTTCGG CCCCAACAGT GGCGCAATGC AATTTCTGGA GAACTATGAA
    AGGGAAGACG TAACAGTAGC AGAACTGGAA GGGAACTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTTTGGGTG CAAAAGCCGA AATAGCAACG
    GATAAGCTTT CTTTCCCTCT TGCGGTCGCA ACACGGAATA GCATAGCTAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC AAACACGCCA GTGACAATAG AGAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGCGATTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC TGAAAATAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACCGG AAGTAGGATA TTCAACCAGG GGTCCCAAGC CTTGGAACTG
    GTGGTCTTCA ATGACAAAGT CAGCCCTCCA AGTCTAGGCT TCTTGGCTGG CTATGGTATC
    ATGGGATTGT ATGCATCTGT TGTCCTTGTA ATTGGGAAAT TTGTTCGTGA GTTCTTCAGT
    GGGATCTCTC ATTCCATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT CTTGAAGTTG
    TGCACAGATA TATTCCTCGT GAGGGAGACA GGGGAGTTGG AACTGGAGGA AGACCTCTAC
    GCCAAGTTGA TATTCCTGTA CAGGTCACCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526230.1
    CDS1448..9976
    Translation

    Target ORF information:

    RefSeq Version XM_006526167.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526167.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGATGAGGAG CTCCAAGATG TACAAGTGGA AGGGGAGCCC
    ACAGAGAAGG AAGAAGAAGA GGAAGAGGAA ATAAAGGAAG AAAGGCATGA GGTAAAGAAG
    GAAGAGGAAG AGGAAGTGGA GGAAGATGAT GACCAGGACA TCATGAAGGT GCTGGGTAAT
    CTGGTGGTGG CCCTCTTCAT CAAGTATTGG ATCTACGTCT GCGGGGGCAT GTTCTTTTTT
    GTCAGCTTTG AGGGGAAAAT CGTCATGTAC AAAATCATCT ACATGGTGCT GTTCCTGTTC
    TGCGTGGCCT TGTACCAGGT GCACTACGAG TGGTGGAGGA AGATCCTGAA GTACTTCTGG
    ATGTCGGTGG TCATTTATAC CATGTTGGTG CTCATCTTCA TATACACTTA CCAGTTTGAA
    AACTTCCCAG GCCTGTGGCA AAACATGACC GGATTAAAGA AAGAAAAGCT CGAGGACCTC
    GGCCTGAAGC AGTTCACGGT CGCTGAACTG TTCACTCGCA TATTCATCCC CACCTCCTTT
    CTACTCGTCT GCATCCTACA CCTCCACTAC TTCCACGATC GGTTCCTGGA ACTTACAGAC
    CTCAAGTCCA TCCCCAGCAA AGAAGACAAC ACCATCTACA GGTTGGCCCA CCCTGAAGGA
    AGTCTTCCAG ACTTAGCCAT CATGAATATG ACGGCCAGCC TTGATAAGCC TGAGGTGCAG
    AAGTTGGCAG AGTCTGGGGA GGAGAGGCCA GAAGAGTGTG TCAAGAAGAC GGAGAAGGGT
    GAGGCTGGGA AAGACAGTGA TGAGTCTGAG GAGGAGGAAG ATGAAGAAGA GGAATCCGAG
    GAAGAAGAAA GCTCAGACCT CAGGAACAAG TGGCACCTGG TGATTGACCG GCTCACTGTG
    CTCTTTCTAA AATTCCTGGA GTATTTCCAC AAGTTGCAGG TGTTTATGTG GTGGATCTTG
    GAGCTGCACA TCATCAAAAT CGTCTCATCG TACATTATCT GGGTGACTGT GAAAGAGGTG
    TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCATTTG CTCTGCCATA TGCCAAACTC
    CGTCGTGCGG CTTCCAGTGT CTGCACTGTC TGGACATGTG TGATCATCGT CTGCAAAATG
    TTGTACCAGC TGCAAACCAT TAAACCTGAG AACTTTTCTG TCAACTGCTC CTTGCCCAAT
    GAAAATCAAA CCAACATTCC CCTTCATGAG TTGAACAAGT CTCTTCTCTA CAGTGCCCCT
    GTTGATCCCA CTGAGTGGGT AGGCCTGAGG AAGTCCTCTC CTTTGCTCGT CTACCTGAGG
    AACAACCTCC TGATGCTGGC GATTTTGGCC TTTGAAGTCA CCGTTTACCG CCATCAGGAG
    TACTATCGAG GCCGAAACAA CCTGACTGCT CCAGTGTCTA AAACTATCTT TCATGACATC
    ACAAGGTTAC ATCTAGATGA TGGGCTTATC AATTGTGCCA AATATTTTGT AAATTACTTC
    TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCCG TGAATGTAAT TGGACAGCGA
    ATGGACTTCT ACGCCATGAT TCATGCCTGT TGGTTGATCG GTGTCTTGTA TCGACGCAGA
    AGAAAAGCCA TTGCCGAGGT CTGGCCCAAG TACTGCTGCT TCCTGGCTTG CATCATCACC
    TTCCAGTACT TCGTCTGCAT CGGCATCCCC CCTGCCCCTT GCCGAGATTA TCCATGGAGA
    TTTAAGGGAG CCTACTTCAA TGATAACATT ATCAAGTGGC TGTACTTCCC GGATTTCATT
    GTGCGGCCCA ACCCCGTGTT TCTTGTCTAT GACTTCATGC TGCTCCTGTG TGCTTCCTTA
    CAACGACAGA TCTTTGAGGA CGAAAACAAG GCAGCCGTGC GCATCATGGC CGGGGACAAT
    GTCGAGATCT GCATGAACCT TGATGCAGCC TCGTTCAGCC AGCATAACCC TGTGCCAGAT
    TTCATTCACT GCAGATCTTA CTTGGACATG TCCAAAGTGA TCATCTTCAG CTACCTCTTC
    TGGTTCGTCC TCACCATCAT CTTCATTACG GGAACAACCA GGATCAGCAT ATTTTGCATG
    GGCTACTTGG TGGCCTGTTT CTATTTCCTG CTCTTTGGAG GGGATCTGCT GCTGAAGCCC
    ATCAAGAGCA TCCTTCGTTA CTGGGACTGG CTGATTGCAT ACAACGTGTT TGTGATCACC
    ATGAAGAATA TACTGTCAAT AGGAGCCTGT GGATACATTG GCGCACTAGT AAGAAACAGC
    TGCTGGTTGA TCCAAGCGTT CAGCCTGGCC TGCACCGTGA AAGGCTATCA GATGCCTGAA
    GATGACTCCA GATGCAAGCT GCCCAGTGGG GAGGCAGGGA TCATCTGGGA CAGCATCTGC
    TTTGCGTTTC TGCTGCTGCA GAGGAGAGTC TTCATGAGCT ACTACTTCCT GCATGTCGTG
    GCTGACATTA AAGCATCCCA GATCCTGGCA TCCAGAGGGG CCGAACTTTT CCAGGCTACA
    ATTGTGAAAG CTGTAAAGGC AAGAATTGAA GAAGAGAAAA AATCCATGGA CCAGCTGAAG
    AGACAGATGG ATCGCATCAA AGCCCGGCAA CAAAAGTACA AAAAGGGTAA GGAGAGGATG
    CTGAGCTTGA CCCAGGAGTC AGGGGAAGGC CAGGACATCC AGAAAGTCTC AGAAGAAGAT
    GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAGGGCAAAA AAAAGCAGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTTAAAAAG GAGGATGAAG AACCCCCACG GAAGTCAGCA
    TTCCAGAGAG CTATTGGGAA GTTTGCCTCA GCCATTTTAG CCTTGCCAAA GTCTGTCATT
    AAACTTCCCA AAACTATTCT TCAGTATTTA ATCAGAGCTG CAAAGTTCGT CTACCAAGCC
    TGGATTACTG ATCCTAAAAC GGCACTCCGA CAGAGGAGGA AAGAGAAGAA AAAGTTGGCC
    AGAGAAGAGC AGAAGGAGAG ACGCAAAGGA TCTGGGGACG GTCCAGTGGA ATGGGAAGAT
    CGAGAGGATG AGCCAGTCAA AAAGAAATCT GATGGACCAG ATAATATCAT CAAGAGGATA
    TTTAATATCC TGAAGTTCAC ATGGGTTCTG TTCCTGGCCA CAGTGGATAG TTTCACCACT
    TGGCTCAACT CCATCTCAAG GGAACACATC GACATATCCA CAGTCCTGCG GATTGAAAGA
    TGCATGCTGA CTCGAGAGAT AAAAAAGGGC AATGTTCCAA CACGGGAAAG CATCCACATG
    TACTACCAGA ACCACATTAT GAACCTTTCC CGAGAATCCG GCCTGGACAC AATCGACGAG
    CACTCGGGGG CTGGCTCCAG GGCCCAGGCA GCACATAGGA TGGATAGCTT AGATTCCCGG
    GACAGTATCT CCAGCTGCTA CACTGAAGCG ACCCTGCTGA TCTCAAGGCA GTCCACCCTT
    GATGATTTAG ATGGACAAGA CCCCGTTCCT AAAACAAGCG AGCGTGCTCG GCCTAGGCTC
    CGTAAAATGT TCAGCCTGGA CATGTCCTCT TCGTCAGCTG ACAGTGGCAG TGTAGCATCA
    AGCGAGCCCA CACAGTGTAC CATGCTGTAT TCGCGCCAAG GAACTACGGA AACCATCGAG
    GAGGTGGAGG CTGAAGCTGA GGAGGAGGTG GTAGAAGGTC TGGAGCCTGA GCTCCATGAT
    GCAGAAGAAA AGGAATACGC AGCTGAATAT GAAGCAGGGG TAGAGGAGAT CAGCCTCACT
    CCAGATGAGG AATTGCCTCA GTTCTCTACA GATGACTGTG ATGTGGAGGC GCCGCCCTCT
    TACAGCAAGG CTGTCAGCTT TGAGCATCTA TCGTTTGCGT CGCAAGATGA CTCTGGGGCC
    AAGAACCACA TGGTGGTCAG TCCTGATGAC AGCCGTACAG ACAAGCTGGA GTCAAGCATC
    TTACCACCTC TGACTCATGA GCTGACAGCC AGCGATCTGC TGATGAGCAA GATGTTCCAT
    GATGATGAGC TTGAGGAGTC TGAGAAGTTC TACGTGGACC AGCCCCGGTT TTTGCTGCTA
    TTCTATGCCA TGTATAACAC CCTGGTGGCC CGCTCAGAAA TGGTGTGCTA TTTCGTGATT
    ATCCTGAACC ACATGACCTC TGCCTCCATC ATCACTCTCC TGTTGCCCAT CTTGATCTTC
    CTCTGGGCCA TGCTGTCTGT TCCCAGGCCC AGCCGACGCT TCTGGATGAT GGCCATCGTC
    TACACTGAGG TGGCGATCGT AGTGAAATAC TTCTTCCAGT TCGGCTTCTT TCCCTGGAAT
    AAGGACTTGG AAATATACAA GGAAAGGCCC TACTTTCCAC CGAACATCAT CGGGGTGGAG
    AAGAAGGAGG GCTATGTTCT TTATGACCTC ATTCAGCTGC TGGCTCTATT CTTCCATCGC
    TCCATTCTGA AGTGTCATGG TCTATGGGAC GAGGATGACA TTGTTGACAG TAACACAGAC
    AAGGAAGGAT CGGATGACGA GCTGTCCCTC GATCAGGGCA GAAGAGGCTC CTCAGACTCA
    CTGAAGTCTA TCAACCTGGC TGCCTCTGTA GAGTCTGTCC ACGTGACCTT CCCCGAGCAG
    CCAGCGGCCA TCAGGAGAAA ACGCTCCTGC AGTAGCTCTC AGATCTCCCC ACGGTCCAGC
    TTCTCTTCAA ATAGGTCCAA AAGAGGCAGC ACAAGCACCC GGAACAGTAG CCAGAAAGGA
    AGCAGTGTTT TAAGCCTTAA ACAGAAAAGC AAACGAGAAC TTTACATGGA AAAGCTTCAA
    GAGCATTTAA TCAAAGCAAA GGCGTTTACC ATAAAGAAGA CTCTACAGAT CTATGTCCCC
    ATTCGGCAAT TCTTCTATGA CCTCATCCAC CCAGACTACA GTGCTGTGAC TGATGTATAC
    GTGCTGATGT TTCTGGCTGA CACTGTAGAC TTCATTATCA TCGTCTTTGG ATTCTGGGCC
    TTTGGGAAAC ACTCGGCAGC TGCAGATATC ACCTCTTCTC TATCAGAAGA CCAGGTCCCC
    GGGCCATTCC TGGTGATGGT CCTCATTCAG TTTGGAACCA TGGTGGTGGA CCGTGCACTC
    TACCTCAGGA AGACTGTGCT GGGCAAGGTC ATCTTCCAGG TCATCCTCGT GTTTGGGATT
    CACTTCTGGA TGTTCTTCAT CTTACCCGGT GTGACTGAGA GGAAATTCAG CCAGAACCTG
    GTGGCACAGC TTTGGTACTT TGTGAAGTGT GTGTACTTTG GGCTGTCTGC CTATCAAATC
    CGCTGTGGCT ACCCAACCCG TGTGCTCGGG AACTTCCTCA CAAAGAGCTA CAATTACGTC
    AACCTCTTCT TGTTTCAAGG GTTCCGCCTG GTCCCATTTC TGACTGAGCT GCGGGCTGTG
    ATGGATTGGG TGTGGACAGA TACAACCCTG AGCCTCTCCA GCTGGATTTG TGTGGAGGAC
    ATTTACGCGC ACATTTTTAT CTTAAAGTGC TGGCGAGAGT CGGAAAAGAG ATACCCTCAA
    CCCAGGGGTC AGAAGAAGAA GAAAGCCGTG AAGTACGGCA TGGGCGGCAT GATCATCGTC
    CTTCTCATCT GTATCGTCTG GTTTCCTCTG CTCTTCATGT CTTTGATCAA ATCTGTCGCA
    GGGGTTATCA ACCAACCCCT GGATGTGTCC GTCACAATTA CCCTGGGAGG TTATCAGCCT
    ATCTTCACAA TGAGCGCCCA GCAAAGCCAG CTGAAAGTTA TGGATAATTC AAAGTATAAT
    GAATTTCTAA AATCTTTCGG CCCCAACAGT GGCGCAATGC AATTTCTGGA GAACTATGAA
    AGGGAAGACG TAACAGTAGC AGAACTGGAA GGGAACTCGA ATTCTTTGTG GACCATCAGC
    CCTCCCAGTA AGCAGAAAAT GATACAGGAG CTCACCGACC CCAATAGTTG CTTCTCTGTT
    GTTTTTTCAT GGAGTATCCA GAGAAACATG ACTTTGGGTG CAAAAGCCGA AATAGCAACG
    GATAAGCTTT CTTTCCCTCT TGCGGTCGCA ACACGGAATA GCATAGCTAA AATGATTGCT
    GGCAATGACA CAGAAAGTTC AAACACGCCA GTGACAATAG AGAAGATCTA CCCATACTAT
    GTGAAGGCAC CCAGCGATTC AAACTCAAAG CCTATAAAAC AGCTTCTGTC TGAAAATAAT
    TTCATGAATA TCACCATCAT TTTGTTCAGA GACAATGTCA CAAAGTCCAA TAGTGAGTGG
    TGGGTTCTCA ACCTGACCGG AAGTAGGATA TTCAACCAGG GGTCCCAAGC CTTGGAACTG
    GTGGTCTTCA ATGACAAAGT CAGCCCTCCA AGTCTAGGCT TCTTGGCTGG CTATGGTATC
    ATGGGATTGT ATGCATCTGT TGTCCTTGTA ATTGGGAAAT TTGTTCGTGA GTTCTTCAGT
    GGGATCTCTC ATTCCATCAT GTTTGAAGAG CTTCCAAATG TGGACAGAAT CTTGAAGTTG
    TGCACAGATA TATTCCTCGT GAGGGAGACA GGGGAGTTGG AACTGGAGGA AGACCTCTAC
    GCCAAGTTGA TATTCCTGTA CAGGTCACCA GAAACCATGA TCAAGTGGAC TAGAGAAAAA
    ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54734
    Clone ID Related Accession (Same CDS sequence) XM_006526168.4 , XM_006526168.3
    Accession Version XM_006526168.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526168.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACCGTGAA TGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGCCTG
    CCCATAGAGA ATGGCCCTGC CAAGACGGAC CTGTACACCA CTCCCCAGTA CCGGTGGGAG
    CCTTCGGAGG AGTCCTCAGA AAAGAAAGAG GAAGAAGAGG ACAAGAGAGA AGACTCCGAA
    GGGGAAGGGA GCCAGGAGGA GAAAAGGAGT GTGAGAATGC ACGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC TTTCGTGCTG TTGATCTGGT CCTGCACCCT CTGGATGATT
    CGCAACAGGA GGAAGTATGC CATGATCAGT TCTCCTTTCA TGGTCGTCTA TGCGAATCTG
    CTGCTGGTGC TGCAGTACAT ATGGAGCTTC GAGCTCCCCG AGATTAAGAA GGTCCCCGGG
    TTTCTAGAAA AGAAAGAGCC GGGAGAACTT GCTTCAAAGA TTCTCTTCAC CATCACGTTC
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TGAGGGAAAA GGAAGCACTT
    CTTTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGAAGAAA AGGAAGATGA GGAGCTCCAA
    GATGTACAAG TGGAAGGGGA GCCCACAGAG AAGGAAGAAG AAGAGGAAGA GGAAATAAAG
    GAAGAAAGGC ATGAGGTAAA GAAGGAAGAG GAAGAGGAAG TGGAGGAAGA TGATGACCAG
    GACATCATGA AGGTGCTGGG TAATCTGGTG GTGGCCCTCT TCATCAAGTA TTGGATCTAC
    GTCTGCGGGG GCATGTTCTT TTTTGTCAGC TTTGAGGGGA AAATCGTCAT GTACAAAATC
    ATCTACATGG TGCTGTTCCT GTTCTGCGTG GCCTTGTACC AGGTGCACTA CGAGTGGTGG
    AGGAAGATCC TGAAGTACTT CTGGATGTCG GTGGTCATTT ATACCATGTT GGTGCTCATC
    TTCATATACA CTTACCAGTT TGAAAACTTC CCAGGCCTGT GGCAAAACAT GACCGGATTA
    AAGAAAGAAA AGCTCGAGGA CCTCGGCCTG AAGCAGTTCA CGGTCGCTGA ACTGTTCACT
    CGCATATTCA TCCCCACCTC CTTTCTACTC GTCTGCATCC TACACCTCCA CTACTTCCAC
    GATCGGTTCC TGGAACTTAC AGACCTCAAG TCCATCCCCA GCAAAGAAGA CAACACCATC
    TACAGGTTGG CCCACCCTGA AGGAAGTCTT CCAGACTTAG CCATCATGAA TATGACGGCC
    AGCCTTGATA AGCCTGAGGT GCAGAAGTTG GCAGAGTCTG GGGAGGAGAG GCCAGAAGAG
    TGTGTCAAGA AGACGGAGAA GGGTGAGGCT GGGAAAGACA GTGATGAGTC TGAGGAGGAG
    GAAGATGAAG AAGAGGAATC CGAGGAAGAA GAAAGCTCAG ACCTCAGGAA CAAGTGGCAC
    CTGGTGATTG ACCGGCTCAC TGTGCTCTTT CTAAAATTCC TGGAGTATTT CCACAAGTTG
    CAGGTGTTTA TGTGGTGGAT CTTGGAGCTG CACATCATCA AAATCGTCTC ATCGTACATT
    ATCTGGGTGA CTGTGAAAGA GGTGTCTCTG TTCAACTATG TATTTTTGAT TTCTTGGGCA
    TTTGCTCTGC CATATGCCAA ACTCCGTCGT GCGGCTTCCA GTGTCTGCAC TGTCTGGACA
    TGTGTGATCA TCGTCTGCAA AATGTTGTAC CAGCTGCAAA CCATTAAACC TGAGAACTTT
    TCTGTCAACT GCTCCTTGCC CAATGAAAAT CAAACCAACA TTCCCCTTCA TGAGTTGAAC
    AAGTCTCTTC TCTACAGTGC CCCTGTTGAT CCCACTGAGT GGGTAGGCCT GAGGAAGTCC
    TCTCCTTTGC TCGTCTACCT GAGGAACAAC CTCCTGATGC TGGCGATTTT GGCCTTTGAA
    GTCACCGTTT ACCGCCATCA GGAGTACTAT CGAGGCCGAA ACAACCTGAC TGCTCCAGTG
    TCTAAAACTA TCTTTCATGA CATCACAAGG TTACATCTAG ATGATGGGCT TATCAATTGT
    GCCAAATATT TTGTAAATTA CTTCTTCTAC AAGTTTGGCC TAGAGACCTG TTTCCTAATG
    TCCGTGAATG TAATTGGACA GCGAATGGAC TTCTACGCCA TGATTCATGC CTGTTGGTTG
    ATCGGTGTCT TGTATCGACG CAGAAGAAAA GCCATTGCCG AGGTCTGGCC CAAGTACTGC
    TGCTTCCTGG CTTGCATCAT CACCTTCCAG TACTTCGTCT GCATCGGCAT CCCCCCTGCC
    CCTTGCCGAG ATTATCCATG GAGATTTAAG GGAGCCTACT TCAATGATAA CATTATCAAG
    TGGCTGTACT TCCCGGATTT CATTGTGCGG CCCAACCCCG TGTTTCTTGT CTATGACTTC
    ATGCTGCTCC TGTGTGCTTC CTTACAACGA CAGATCTTTG AGGACGAAAA CAAGGCAGCC
    GTGCGCATCA TGGCCGGGGA CAATGTCGAG ATCTGCATGA ACCTTGATGC AGCCTCGTTC
    AGCCAGCATA ACCCTGTGCC AGATTTCATT CACTGCAGAT CTTACTTGGA CATGTCCAAA
    GTGATCATCT TCAGCTACCT CTTCTGGTTC GTCCTCACCA TCATCTTCAT TACGGGAACA
    ACCAGGATCA GCATATTTTG CATGGGCTAC TTGGTGGCCT GTTTCTATTT CCTGCTCTTT
    GGAGGGGATC TGCTGCTGAA GCCCATCAAG AGCATCCTTC GTTACTGGGA CTGGCTGATT
    GCATACAACG TGTTTGTGAT CACCATGAAG AATATACTGT CAATAGGAGC CTGTGGATAC
    ATTGGCGCAC TAGTAAGAAA CAGCTGCTGG TTGATCCAAG CGTTCAGCCT GGCCTGCACC
    GTGAAAGGCT ATCAGATGCC TGAAGATGAC TCCAGATGCA AGCTGCCCAG TGGGGAGGCA
    GGGATCATCT GGGACAGCAT CTGCTTTGCG TTTCTGCTGC TGCAGAGGAG AGTCTTCATG
    AGCTACTACT TCCTGCATGT CGTGGCTGAC ATTAAAGCAT CCCAGATCCT GGCATCCAGA
    GGGGCCGAAC TTTTCCAGGC TACAATTGTG AAAGCTGTAA AGGCAAGAAT TGAAGAAGAG
    AAAAAATCCA TGGACCAGCT GAAGAGACAG ATGGATCGCA TCAAAGCCCG GCAACAAAAG
    TACAAAAAGG GTAAGGAGAG GATGCTGAGC TTGACCCAGG AGTCAGGGGA AGGCCAGGAC
    ATCCAGAAAG TCTCAGAAGA AGATGATGAA AGAGAAGCAG ACAAACAGAA AGCCAAGGGC
    AAAAAAAAGC AGTGGTGGCG GCCTTGGGTT GATCATGCTT CCATGGTCAG GAGTGGAGAT
    TATTATTTGT TTGAAACGGA TAGTGAAGAG GAGGAAGAAG AGGAACTTAA AAAGGAGGAT
    GAAGAACCCC CACGGAAGTC AGCATTCCAG AGAGCTATTG GGAAGTTTGC CTCAGCCATT
    TTAGCCTTGC CAAAGTCTGT CATTAAACTT CCCAAAACTA TTCTTCAGTA TTTAATCAGA
    GCTGCAAAGT TCGTCTACCA AGCCTGGATT ACTGATCCTA AAACGGCACT CCGACAGAGG
    AGGAAAGAGA AGAAAAAGTT GGCCAGAGAA GAGCAGAAGG AGAGACGCAA AGGATCTGGG
    GACGGTCCAG TGGAATGGGA AGATCGAGAG GATGAGCCAG TCAAAAAGAA ATCTGATGGA
    CCAGATAATA TCATCAAGAG GATATTTAAT ATCCTGAAGT TCACATGGGT TCTGTTCCTG
    GCCACAGTGG ATAGTTTCAC CACTTGGCTC AACTCCATCT CAAGGGAACA CATCGACATA
    TCCACAGTCC TGCGGATTGA AAGATGCATG CTGACTCGAG AGATAAAAAA GGGCAATGTT
    CCAACACGGG AAAGCATCCA CATGTACTAC CAGAACCACA TTATGAACCT TTCCCGAGAA
    TCCGGCCTGG ACACAATCGA CGAGCACTCG GGGGCTGGCT CCAGGGCCCA GGCAGCACAT
    AGGATGGATA GCTTAGATTC CCGGGACAGT ATCTCCAGCT GCTACACTGA AGCGACCCTG
    CTGATCTCAA GGCAGTCCAC CCTTGATGAT TTAGATGGAC AAGACCCCGT TCCTAAAACA
    AGCGAGCGTG CTCGGCCTAG GCTCCGTAAA ATGTTCAGCC TGGACATGTC CTCTTCGTCA
    GCTGACAGTG GCAGTGTAGC ATCAAGCGAG CCCACACAGT GTACCATGCT GTATTCGCGC
    CAAGGAACTA CGGAAACCAT CGAGGAGGTG GAGGCTGAAG CTGAGGAGGA GGTGGTAGAA
    GGTCTGGAGC CTGAGCTCCA TGATGCAGAA GAAAAGGAAT ACGCAGCTGA ATATGAAGCA
    GGGGTAGAGG AGATCAGCCT CACTCCAGAT GAGGAATTGC CTCAGTTCTC TACAGATGAC
    TGTGATGTGG AGGCGCCGCC CTCTTACAGC AAGGCTGTCA GCTTTGAGCA TCTATCGTTT
    GCGTCGCAAG ATGACTCTGG GGCCAAGAAC CACATGGTGG TCAGTCCTGA TGACAGCCGT
    ACAGACAAGC TGGAGTCAAG CATCTTACCA CCTCTGACTC ATGAGCTGAC AGCCAGCGAT
    CTGCTGATGA GCAAGATGTT CCATGATGAT GAGCTTGAGG AGTCTGAGAA GTTCTACGTG
    GACCAGCCCC GGTTTTTGCT GCTATTCTAT GCCATGTATA ACACCCTGGT GGCCCGCTCA
    GAAATGGTGT GCTATTTCGT GATTATCCTG AACCACATGA CCTCTGCCTC CATCATCACT
    CTCCTGTTGC CCATCTTGAT CTTCCTCTGG GCCATGCTGT CTGTTCCCAG GCCCAGCCGA
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TCGTAGTGAA ATACTTCTTC
    CAGTTCGGCT TCTTTCCCTG GAATAAGGAC TTGGAAATAT ACAAGGAAAG GCCCTACTTT
    CCACCGAACA TCATCGGGGT GGAGAAGAAG GAGGGCTATG TTCTTTATGA CCTCATTCAG
    CTGCTGGCTC TATTCTTCCA TCGCTCCATT CTGAAGTGTC ATGGTCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCGGATG ACGAGCTGTC CCTCGATCAG
    GGCAGAAGAG GCTCCTCAGA CTCACTGAAG TCTATCAACC TGGCTGCCTC TGTAGAGTCT
    GTCCACGTGA CCTTCCCCGA GCAGCCAGCG GCCATCAGGA GAAAACGCTC CTGCAGTAGC
    TCTCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGGAACA GTAGCCAGAA AGGAAGCAGT GTTTTAAGCC TTAAACAGAA AAGCAAACGA
    GAACTTTACA TGGAAAAGCT TCAAGAGCAT TTAATCAAAG CAAAGGCGTT TACCATAAAG
    AAGACTCTAC AGATCTATGT CCCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGATGT ATACGTGCTG ATGTTTCTGG CTGACACTGT AGACTTCATT
    ATCATCGTCT TTGGATTCTG GGCCTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCTCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTCCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTGGGCAA GGTCATCTTC
    CAGGTCATCC TCGTGTTTGG GATTCACTTC TGGATGTTCT TCATCTTACC CGGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTTTGGT ACTTTGTGAA GTGTGTGTAC
    TTTGGGCTGT CTGCCTATCA AATCCGCTGT GGCTACCCAA CCCGTGTGCT CGGGAACTTC
    CTCACAAAGA GCTACAATTA CGTCAACCTC TTCTTGTTTC AAGGGTTCCG CCTGGTCCCA
    TTTCTGACTG AGCTGCGGGC TGTGATGGAT TGGGTGTGGA CAGATACAAC CCTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCGCACATTT TTATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGTCAGAAGA AGAAGAAAGC CGTGAAGTAC
    GGCATGGGCG GCATGATCAT CGTCCTTCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAATCTGT CGCAGGGGTT ATCAACCAAC CCCTGGATGT GTCCGTCACA
    ATTACCCTGG GAGGTTATCA GCCTATCTTC ACAATGAGCG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATA ATTCAAAGTA TAATGAATTT CTAAAATCTT TCGGCCCCAA CAGTGGCGCA
    ATGCAATTTC TGGAGAACTA TGAAAGGGAA GACGTAACAG TAGCAGAACT GGAAGGGAAC
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTTTG
    GGTGCAAAAG CCGAAATAGC AACGGATAAG CTTTCTTTCC CTCTTGCGGT CGCAACACGG
    AATAGCATAG CTAAAATGAT TGCTGGCAAT GACACAGAAA GTTCAAACAC GCCAGTGACA
    ATAGAGAAGA TCTACCCATA CTATGTGAAG GCACCCAGCG ATTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCTGAAAA TAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CCGGAAGTAG GATATTCAAC
    CAGGGGTCCC AAGCCTTGGA ACTGGTGGTC TTCAATGACA AAGTCAGCCC TCCAAGTCTA
    GGCTTCTTGG CTGGCTATGG TATCATGGGA TTGTATGCAT CTGTTGTCCT TGTAATTGGG
    AAATTTGTTC GTGAGTTCTT CAGTGGGATC TCTCATTCCA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATCTTGAA GTTGTGCACA GATATATTCC TCGTGAGGGA GACAGGGGAG
    TTGGAACTGG AGGAAGACCT CTACGCCAAG TTGATATTCC TGTACAGGTC ACCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526231.1
    CDS1448..9940
    Translation

    Target ORF information:

    RefSeq Version XM_006526168.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526168.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACCGTGAA TGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGCCTG
    CCCATAGAGA ATGGCCCTGC CAAGACGGAC CTGTACACCA CTCCCCAGTA CCGGTGGGAG
    CCTTCGGAGG AGTCCTCAGA AAAGAAAGAG GAAGAAGAGG ACAAGAGAGA AGACTCCGAA
    GGGGAAGGGA GCCAGGAGGA GAAAAGGAGT GTGAGAATGC ACGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC TTTCGTGCTG TTGATCTGGT CCTGCACCCT CTGGATGATT
    CGCAACAGGA GGAAGTATGC CATGATCAGT TCTCCTTTCA TGGTCGTCTA TGCGAATCTG
    CTGCTGGTGC TGCAGTACAT ATGGAGCTTC GAGCTCCCCG AGATTAAGAA GGTCCCCGGG
    TTTCTAGAAA AGAAAGAGCC GGGAGAACTT GCTTCAAAGA TTCTCTTCAC CATCACGTTC
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TGAGGGAAAA GGAAGCACTT
    CTTTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGAAGAAA AGGAAGATGA GGAGCTCCAA
    GATGTACAAG TGGAAGGGGA GCCCACAGAG AAGGAAGAAG AAGAGGAAGA GGAAATAAAG
    GAAGAAAGGC ATGAGGTAAA GAAGGAAGAG GAAGAGGAAG TGGAGGAAGA TGATGACCAG
    GACATCATGA AGGTGCTGGG TAATCTGGTG GTGGCCCTCT TCATCAAGTA TTGGATCTAC
    GTCTGCGGGG GCATGTTCTT TTTTGTCAGC TTTGAGGGGA AAATCGTCAT GTACAAAATC
    ATCTACATGG TGCTGTTCCT GTTCTGCGTG GCCTTGTACC AGGTGCACTA CGAGTGGTGG
    AGGAAGATCC TGAAGTACTT CTGGATGTCG GTGGTCATTT ATACCATGTT GGTGCTCATC
    TTCATATACA CTTACCAGTT TGAAAACTTC CCAGGCCTGT GGCAAAACAT GACCGGATTA
    AAGAAAGAAA AGCTCGAGGA CCTCGGCCTG AAGCAGTTCA CGGTCGCTGA ACTGTTCACT
    CGCATATTCA TCCCCACCTC CTTTCTACTC GTCTGCATCC TACACCTCCA CTACTTCCAC
    GATCGGTTCC TGGAACTTAC AGACCTCAAG TCCATCCCCA GCAAAGAAGA CAACACCATC
    TACAGGTTGG CCCACCCTGA AGGAAGTCTT CCAGACTTAG CCATCATGAA TATGACGGCC
    AGCCTTGATA AGCCTGAGGT GCAGAAGTTG GCAGAGTCTG GGGAGGAGAG GCCAGAAGAG
    TGTGTCAAGA AGACGGAGAA GGGTGAGGCT GGGAAAGACA GTGATGAGTC TGAGGAGGAG
    GAAGATGAAG AAGAGGAATC CGAGGAAGAA GAAAGCTCAG ACCTCAGGAA CAAGTGGCAC
    CTGGTGATTG ACCGGCTCAC TGTGCTCTTT CTAAAATTCC TGGAGTATTT CCACAAGTTG
    CAGGTGTTTA TGTGGTGGAT CTTGGAGCTG CACATCATCA AAATCGTCTC ATCGTACATT
    ATCTGGGTGA CTGTGAAAGA GGTGTCTCTG TTCAACTATG TATTTTTGAT TTCTTGGGCA
    TTTGCTCTGC CATATGCCAA ACTCCGTCGT GCGGCTTCCA GTGTCTGCAC TGTCTGGACA
    TGTGTGATCA TCGTCTGCAA AATGTTGTAC CAGCTGCAAA CCATTAAACC TGAGAACTTT
    TCTGTCAACT GCTCCTTGCC CAATGAAAAT CAAACCAACA TTCCCCTTCA TGAGTTGAAC
    AAGTCTCTTC TCTACAGTGC CCCTGTTGAT CCCACTGAGT GGGTAGGCCT GAGGAAGTCC
    TCTCCTTTGC TCGTCTACCT GAGGAACAAC CTCCTGATGC TGGCGATTTT GGCCTTTGAA
    GTCACCGTTT ACCGCCATCA GGAGTACTAT CGAGGCCGAA ACAACCTGAC TGCTCCAGTG
    TCTAAAACTA TCTTTCATGA CATCACAAGG TTACATCTAG ATGATGGGCT TATCAATTGT
    GCCAAATATT TTGTAAATTA CTTCTTCTAC AAGTTTGGCC TAGAGACCTG TTTCCTAATG
    TCCGTGAATG TAATTGGACA GCGAATGGAC TTCTACGCCA TGATTCATGC CTGTTGGTTG
    ATCGGTGTCT TGTATCGACG CAGAAGAAAA GCCATTGCCG AGGTCTGGCC CAAGTACTGC
    TGCTTCCTGG CTTGCATCAT CACCTTCCAG TACTTCGTCT GCATCGGCAT CCCCCCTGCC
    CCTTGCCGAG ATTATCCATG GAGATTTAAG GGAGCCTACT TCAATGATAA CATTATCAAG
    TGGCTGTACT TCCCGGATTT CATTGTGCGG CCCAACCCCG TGTTTCTTGT CTATGACTTC
    ATGCTGCTCC TGTGTGCTTC CTTACAACGA CAGATCTTTG AGGACGAAAA CAAGGCAGCC
    GTGCGCATCA TGGCCGGGGA CAATGTCGAG ATCTGCATGA ACCTTGATGC AGCCTCGTTC
    AGCCAGCATA ACCCTGTGCC AGATTTCATT CACTGCAGAT CTTACTTGGA CATGTCCAAA
    GTGATCATCT TCAGCTACCT CTTCTGGTTC GTCCTCACCA TCATCTTCAT TACGGGAACA
    ACCAGGATCA GCATATTTTG CATGGGCTAC TTGGTGGCCT GTTTCTATTT CCTGCTCTTT
    GGAGGGGATC TGCTGCTGAA GCCCATCAAG AGCATCCTTC GTTACTGGGA CTGGCTGATT
    GCATACAACG TGTTTGTGAT CACCATGAAG AATATACTGT CAATAGGAGC CTGTGGATAC
    ATTGGCGCAC TAGTAAGAAA CAGCTGCTGG TTGATCCAAG CGTTCAGCCT GGCCTGCACC
    GTGAAAGGCT ATCAGATGCC TGAAGATGAC TCCAGATGCA AGCTGCCCAG TGGGGAGGCA
    GGGATCATCT GGGACAGCAT CTGCTTTGCG TTTCTGCTGC TGCAGAGGAG AGTCTTCATG
    AGCTACTACT TCCTGCATGT CGTGGCTGAC ATTAAAGCAT CCCAGATCCT GGCATCCAGA
    GGGGCCGAAC TTTTCCAGGC TACAATTGTG AAAGCTGTAA AGGCAAGAAT TGAAGAAGAG
    AAAAAATCCA TGGACCAGCT GAAGAGACAG ATGGATCGCA TCAAAGCCCG GCAACAAAAG
    TACAAAAAGG GTAAGGAGAG GATGCTGAGC TTGACCCAGG AGTCAGGGGA AGGCCAGGAC
    ATCCAGAAAG TCTCAGAAGA AGATGATGAA AGAGAAGCAG ACAAACAGAA AGCCAAGGGC
    AAAAAAAAGC AGTGGTGGCG GCCTTGGGTT GATCATGCTT CCATGGTCAG GAGTGGAGAT
    TATTATTTGT TTGAAACGGA TAGTGAAGAG GAGGAAGAAG AGGAACTTAA AAAGGAGGAT
    GAAGAACCCC CACGGAAGTC AGCATTCCAG AGAGCTATTG GGAAGTTTGC CTCAGCCATT
    TTAGCCTTGC CAAAGTCTGT CATTAAACTT CCCAAAACTA TTCTTCAGTA TTTAATCAGA
    GCTGCAAAGT TCGTCTACCA AGCCTGGATT ACTGATCCTA AAACGGCACT CCGACAGAGG
    AGGAAAGAGA AGAAAAAGTT GGCCAGAGAA GAGCAGAAGG AGAGACGCAA AGGATCTGGG
    GACGGTCCAG TGGAATGGGA AGATCGAGAG GATGAGCCAG TCAAAAAGAA ATCTGATGGA
    CCAGATAATA TCATCAAGAG GATATTTAAT ATCCTGAAGT TCACATGGGT TCTGTTCCTG
    GCCACAGTGG ATAGTTTCAC CACTTGGCTC AACTCCATCT CAAGGGAACA CATCGACATA
    TCCACAGTCC TGCGGATTGA AAGATGCATG CTGACTCGAG AGATAAAAAA GGGCAATGTT
    CCAACACGGG AAAGCATCCA CATGTACTAC CAGAACCACA TTATGAACCT TTCCCGAGAA
    TCCGGCCTGG ACACAATCGA CGAGCACTCG GGGGCTGGCT CCAGGGCCCA GGCAGCACAT
    AGGATGGATA GCTTAGATTC CCGGGACAGT ATCTCCAGCT GCTACACTGA AGCGACCCTG
    CTGATCTCAA GGCAGTCCAC CCTTGATGAT TTAGATGGAC AAGACCCCGT TCCTAAAACA
    AGCGAGCGTG CTCGGCCTAG GCTCCGTAAA ATGTTCAGCC TGGACATGTC CTCTTCGTCA
    GCTGACAGTG GCAGTGTAGC ATCAAGCGAG CCCACACAGT GTACCATGCT GTATTCGCGC
    CAAGGAACTA CGGAAACCAT CGAGGAGGTG GAGGCTGAAG CTGAGGAGGA GGTGGTAGAA
    GGTCTGGAGC CTGAGCTCCA TGATGCAGAA GAAAAGGAAT ACGCAGCTGA ATATGAAGCA
    GGGGTAGAGG AGATCAGCCT CACTCCAGAT GAGGAATTGC CTCAGTTCTC TACAGATGAC
    TGTGATGTGG AGGCGCCGCC CTCTTACAGC AAGGCTGTCA GCTTTGAGCA TCTATCGTTT
    GCGTCGCAAG ATGACTCTGG GGCCAAGAAC CACATGGTGG TCAGTCCTGA TGACAGCCGT
    ACAGACAAGC TGGAGTCAAG CATCTTACCA CCTCTGACTC ATGAGCTGAC AGCCAGCGAT
    CTGCTGATGA GCAAGATGTT CCATGATGAT GAGCTTGAGG AGTCTGAGAA GTTCTACGTG
    GACCAGCCCC GGTTTTTGCT GCTATTCTAT GCCATGTATA ACACCCTGGT GGCCCGCTCA
    GAAATGGTGT GCTATTTCGT GATTATCCTG AACCACATGA CCTCTGCCTC CATCATCACT
    CTCCTGTTGC CCATCTTGAT CTTCCTCTGG GCCATGCTGT CTGTTCCCAG GCCCAGCCGA
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TCGTAGTGAA ATACTTCTTC
    CAGTTCGGCT TCTTTCCCTG GAATAAGGAC TTGGAAATAT ACAAGGAAAG GCCCTACTTT
    CCACCGAACA TCATCGGGGT GGAGAAGAAG GAGGGCTATG TTCTTTATGA CCTCATTCAG
    CTGCTGGCTC TATTCTTCCA TCGCTCCATT CTGAAGTGTC ATGGTCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCGGATG ACGAGCTGTC CCTCGATCAG
    GGCAGAAGAG GCTCCTCAGA CTCACTGAAG TCTATCAACC TGGCTGCCTC TGTAGAGTCT
    GTCCACGTGA CCTTCCCCGA GCAGCCAGCG GCCATCAGGA GAAAACGCTC CTGCAGTAGC
    TCTCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGGAACA GTAGCCAGAA AGGAAGCAGT GTTTTAAGCC TTAAACAGAA AAGCAAACGA
    GAACTTTACA TGGAAAAGCT TCAAGAGCAT TTAATCAAAG CAAAGGCGTT TACCATAAAG
    AAGACTCTAC AGATCTATGT CCCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGATGT ATACGTGCTG ATGTTTCTGG CTGACACTGT AGACTTCATT
    ATCATCGTCT TTGGATTCTG GGCCTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCTCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTCCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTGGGCAA GGTCATCTTC
    CAGGTCATCC TCGTGTTTGG GATTCACTTC TGGATGTTCT TCATCTTACC CGGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTTTGGT ACTTTGTGAA GTGTGTGTAC
    TTTGGGCTGT CTGCCTATCA AATCCGCTGT GGCTACCCAA CCCGTGTGCT CGGGAACTTC
    CTCACAAAGA GCTACAATTA CGTCAACCTC TTCTTGTTTC AAGGGTTCCG CCTGGTCCCA
    TTTCTGACTG AGCTGCGGGC TGTGATGGAT TGGGTGTGGA CAGATACAAC CCTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCGCACATTT TTATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGTCAGAAGA AGAAGAAAGC CGTGAAGTAC
    GGCATGGGCG GCATGATCAT CGTCCTTCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAATCTGT CGCAGGGGTT ATCAACCAAC CCCTGGATGT GTCCGTCACA
    ATTACCCTGG GAGGTTATCA GCCTATCTTC ACAATGAGCG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATA ATTCAAAGTA TAATGAATTT CTAAAATCTT TCGGCCCCAA CAGTGGCGCA
    ATGCAATTTC TGGAGAACTA TGAAAGGGAA GACGTAACAG TAGCAGAACT GGAAGGGAAC
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTTTG
    GGTGCAAAAG CCGAAATAGC AACGGATAAG CTTTCTTTCC CTCTTGCGGT CGCAACACGG
    AATAGCATAG CTAAAATGAT TGCTGGCAAT GACACAGAAA GTTCAAACAC GCCAGTGACA
    ATAGAGAAGA TCTACCCATA CTATGTGAAG GCACCCAGCG ATTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCTGAAAA TAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CCGGAAGTAG GATATTCAAC
    CAGGGGTCCC AAGCCTTGGA ACTGGTGGTC TTCAATGACA AAGTCAGCCC TCCAAGTCTA
    GGCTTCTTGG CTGGCTATGG TATCATGGGA TTGTATGCAT CTGTTGTCCT TGTAATTGGG
    AAATTTGTTC GTGAGTTCTT CAGTGGGATC TCTCATTCCA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATCTTGAA GTTGTGCACA GATATATTCC TCGTGAGGGA GACAGGGGAG
    TTGGAACTGG AGGAAGACCT CTACGCCAAG TTGATATTCC TGTACAGGTC ACCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54734
    Clone ID Related Accession (Same CDS sequence) XM_006526168.4 , XM_006526168.3
    Accession Version XM_006526168.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526168.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACCGTGAA TGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGCCTG
    CCCATAGAGA ATGGCCCTGC CAAGACGGAC CTGTACACCA CTCCCCAGTA CCGGTGGGAG
    CCTTCGGAGG AGTCCTCAGA AAAGAAAGAG GAAGAAGAGG ACAAGAGAGA AGACTCCGAA
    GGGGAAGGGA GCCAGGAGGA GAAAAGGAGT GTGAGAATGC ACGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC TTTCGTGCTG TTGATCTGGT CCTGCACCCT CTGGATGATT
    CGCAACAGGA GGAAGTATGC CATGATCAGT TCTCCTTTCA TGGTCGTCTA TGCGAATCTG
    CTGCTGGTGC TGCAGTACAT ATGGAGCTTC GAGCTCCCCG AGATTAAGAA GGTCCCCGGG
    TTTCTAGAAA AGAAAGAGCC GGGAGAACTT GCTTCAAAGA TTCTCTTCAC CATCACGTTC
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TGAGGGAAAA GGAAGCACTT
    CTTTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGAAGAAA AGGAAGATGA GGAGCTCCAA
    GATGTACAAG TGGAAGGGGA GCCCACAGAG AAGGAAGAAG AAGAGGAAGA GGAAATAAAG
    GAAGAAAGGC ATGAGGTAAA GAAGGAAGAG GAAGAGGAAG TGGAGGAAGA TGATGACCAG
    GACATCATGA AGGTGCTGGG TAATCTGGTG GTGGCCCTCT TCATCAAGTA TTGGATCTAC
    GTCTGCGGGG GCATGTTCTT TTTTGTCAGC TTTGAGGGGA AAATCGTCAT GTACAAAATC
    ATCTACATGG TGCTGTTCCT GTTCTGCGTG GCCTTGTACC AGGTGCACTA CGAGTGGTGG
    AGGAAGATCC TGAAGTACTT CTGGATGTCG GTGGTCATTT ATACCATGTT GGTGCTCATC
    TTCATATACA CTTACCAGTT TGAAAACTTC CCAGGCCTGT GGCAAAACAT GACCGGATTA
    AAGAAAGAAA AGCTCGAGGA CCTCGGCCTG AAGCAGTTCA CGGTCGCTGA ACTGTTCACT
    CGCATATTCA TCCCCACCTC CTTTCTACTC GTCTGCATCC TACACCTCCA CTACTTCCAC
    GATCGGTTCC TGGAACTTAC AGACCTCAAG TCCATCCCCA GCAAAGAAGA CAACACCATC
    TACAGGTTGG CCCACCCTGA AGGAAGTCTT CCAGACTTAG CCATCATGAA TATGACGGCC
    AGCCTTGATA AGCCTGAGGT GCAGAAGTTG GCAGAGTCTG GGGAGGAGAG GCCAGAAGAG
    TGTGTCAAGA AGACGGAGAA GGGTGAGGCT GGGAAAGACA GTGATGAGTC TGAGGAGGAG
    GAAGATGAAG AAGAGGAATC CGAGGAAGAA GAAAGCTCAG ACCTCAGGAA CAAGTGGCAC
    CTGGTGATTG ACCGGCTCAC TGTGCTCTTT CTAAAATTCC TGGAGTATTT CCACAAGTTG
    CAGGTGTTTA TGTGGTGGAT CTTGGAGCTG CACATCATCA AAATCGTCTC ATCGTACATT
    ATCTGGGTGA CTGTGAAAGA GGTGTCTCTG TTCAACTATG TATTTTTGAT TTCTTGGGCA
    TTTGCTCTGC CATATGCCAA ACTCCGTCGT GCGGCTTCCA GTGTCTGCAC TGTCTGGACA
    TGTGTGATCA TCGTCTGCAA AATGTTGTAC CAGCTGCAAA CCATTAAACC TGAGAACTTT
    TCTGTCAACT GCTCCTTGCC CAATGAAAAT CAAACCAACA TTCCCCTTCA TGAGTTGAAC
    AAGTCTCTTC TCTACAGTGC CCCTGTTGAT CCCACTGAGT GGGTAGGCCT GAGGAAGTCC
    TCTCCTTTGC TCGTCTACCT GAGGAACAAC CTCCTGATGC TGGCGATTTT GGCCTTTGAA
    GTCACCGTTT ACCGCCATCA GGAGTACTAT CGAGGCCGAA ACAACCTGAC TGCTCCAGTG
    TCTAAAACTA TCTTTCATGA CATCACAAGG TTACATCTAG ATGATGGGCT TATCAATTGT
    GCCAAATATT TTGTAAATTA CTTCTTCTAC AAGTTTGGCC TAGAGACCTG TTTCCTAATG
    TCCGTGAATG TAATTGGACA GCGAATGGAC TTCTACGCCA TGATTCATGC CTGTTGGTTG
    ATCGGTGTCT TGTATCGACG CAGAAGAAAA GCCATTGCCG AGGTCTGGCC CAAGTACTGC
    TGCTTCCTGG CTTGCATCAT CACCTTCCAG TACTTCGTCT GCATCGGCAT CCCCCCTGCC
    CCTTGCCGAG ATTATCCATG GAGATTTAAG GGAGCCTACT TCAATGATAA CATTATCAAG
    TGGCTGTACT TCCCGGATTT CATTGTGCGG CCCAACCCCG TGTTTCTTGT CTATGACTTC
    ATGCTGCTCC TGTGTGCTTC CTTACAACGA CAGATCTTTG AGGACGAAAA CAAGGCAGCC
    GTGCGCATCA TGGCCGGGGA CAATGTCGAG ATCTGCATGA ACCTTGATGC AGCCTCGTTC
    AGCCAGCATA ACCCTGTGCC AGATTTCATT CACTGCAGAT CTTACTTGGA CATGTCCAAA
    GTGATCATCT TCAGCTACCT CTTCTGGTTC GTCCTCACCA TCATCTTCAT TACGGGAACA
    ACCAGGATCA GCATATTTTG CATGGGCTAC TTGGTGGCCT GTTTCTATTT CCTGCTCTTT
    GGAGGGGATC TGCTGCTGAA GCCCATCAAG AGCATCCTTC GTTACTGGGA CTGGCTGATT
    GCATACAACG TGTTTGTGAT CACCATGAAG AATATACTGT CAATAGGAGC CTGTGGATAC
    ATTGGCGCAC TAGTAAGAAA CAGCTGCTGG TTGATCCAAG CGTTCAGCCT GGCCTGCACC
    GTGAAAGGCT ATCAGATGCC TGAAGATGAC TCCAGATGCA AGCTGCCCAG TGGGGAGGCA
    GGGATCATCT GGGACAGCAT CTGCTTTGCG TTTCTGCTGC TGCAGAGGAG AGTCTTCATG
    AGCTACTACT TCCTGCATGT CGTGGCTGAC ATTAAAGCAT CCCAGATCCT GGCATCCAGA
    GGGGCCGAAC TTTTCCAGGC TACAATTGTG AAAGCTGTAA AGGCAAGAAT TGAAGAAGAG
    AAAAAATCCA TGGACCAGCT GAAGAGACAG ATGGATCGCA TCAAAGCCCG GCAACAAAAG
    TACAAAAAGG GTAAGGAGAG GATGCTGAGC TTGACCCAGG AGTCAGGGGA AGGCCAGGAC
    ATCCAGAAAG TCTCAGAAGA AGATGATGAA AGAGAAGCAG ACAAACAGAA AGCCAAGGGC
    AAAAAAAAGC AGTGGTGGCG GCCTTGGGTT GATCATGCTT CCATGGTCAG GAGTGGAGAT
    TATTATTTGT TTGAAACGGA TAGTGAAGAG GAGGAAGAAG AGGAACTTAA AAAGGAGGAT
    GAAGAACCCC CACGGAAGTC AGCATTCCAG AGAGCTATTG GGAAGTTTGC CTCAGCCATT
    TTAGCCTTGC CAAAGTCTGT CATTAAACTT CCCAAAACTA TTCTTCAGTA TTTAATCAGA
    GCTGCAAAGT TCGTCTACCA AGCCTGGATT ACTGATCCTA AAACGGCACT CCGACAGAGG
    AGGAAAGAGA AGAAAAAGTT GGCCAGAGAA GAGCAGAAGG AGAGACGCAA AGGATCTGGG
    GACGGTCCAG TGGAATGGGA AGATCGAGAG GATGAGCCAG TCAAAAAGAA ATCTGATGGA
    CCAGATAATA TCATCAAGAG GATATTTAAT ATCCTGAAGT TCACATGGGT TCTGTTCCTG
    GCCACAGTGG ATAGTTTCAC CACTTGGCTC AACTCCATCT CAAGGGAACA CATCGACATA
    TCCACAGTCC TGCGGATTGA AAGATGCATG CTGACTCGAG AGATAAAAAA GGGCAATGTT
    CCAACACGGG AAAGCATCCA CATGTACTAC CAGAACCACA TTATGAACCT TTCCCGAGAA
    TCCGGCCTGG ACACAATCGA CGAGCACTCG GGGGCTGGCT CCAGGGCCCA GGCAGCACAT
    AGGATGGATA GCTTAGATTC CCGGGACAGT ATCTCCAGCT GCTACACTGA AGCGACCCTG
    CTGATCTCAA GGCAGTCCAC CCTTGATGAT TTAGATGGAC AAGACCCCGT TCCTAAAACA
    AGCGAGCGTG CTCGGCCTAG GCTCCGTAAA ATGTTCAGCC TGGACATGTC CTCTTCGTCA
    GCTGACAGTG GCAGTGTAGC ATCAAGCGAG CCCACACAGT GTACCATGCT GTATTCGCGC
    CAAGGAACTA CGGAAACCAT CGAGGAGGTG GAGGCTGAAG CTGAGGAGGA GGTGGTAGAA
    GGTCTGGAGC CTGAGCTCCA TGATGCAGAA GAAAAGGAAT ACGCAGCTGA ATATGAAGCA
    GGGGTAGAGG AGATCAGCCT CACTCCAGAT GAGGAATTGC CTCAGTTCTC TACAGATGAC
    TGTGATGTGG AGGCGCCGCC CTCTTACAGC AAGGCTGTCA GCTTTGAGCA TCTATCGTTT
    GCGTCGCAAG ATGACTCTGG GGCCAAGAAC CACATGGTGG TCAGTCCTGA TGACAGCCGT
    ACAGACAAGC TGGAGTCAAG CATCTTACCA CCTCTGACTC ATGAGCTGAC AGCCAGCGAT
    CTGCTGATGA GCAAGATGTT CCATGATGAT GAGCTTGAGG AGTCTGAGAA GTTCTACGTG
    GACCAGCCCC GGTTTTTGCT GCTATTCTAT GCCATGTATA ACACCCTGGT GGCCCGCTCA
    GAAATGGTGT GCTATTTCGT GATTATCCTG AACCACATGA CCTCTGCCTC CATCATCACT
    CTCCTGTTGC CCATCTTGAT CTTCCTCTGG GCCATGCTGT CTGTTCCCAG GCCCAGCCGA
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TCGTAGTGAA ATACTTCTTC
    CAGTTCGGCT TCTTTCCCTG GAATAAGGAC TTGGAAATAT ACAAGGAAAG GCCCTACTTT
    CCACCGAACA TCATCGGGGT GGAGAAGAAG GAGGGCTATG TTCTTTATGA CCTCATTCAG
    CTGCTGGCTC TATTCTTCCA TCGCTCCATT CTGAAGTGTC ATGGTCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCGGATG ACGAGCTGTC CCTCGATCAG
    GGCAGAAGAG GCTCCTCAGA CTCACTGAAG TCTATCAACC TGGCTGCCTC TGTAGAGTCT
    GTCCACGTGA CCTTCCCCGA GCAGCCAGCG GCCATCAGGA GAAAACGCTC CTGCAGTAGC
    TCTCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGGAACA GTAGCCAGAA AGGAAGCAGT GTTTTAAGCC TTAAACAGAA AAGCAAACGA
    GAACTTTACA TGGAAAAGCT TCAAGAGCAT TTAATCAAAG CAAAGGCGTT TACCATAAAG
    AAGACTCTAC AGATCTATGT CCCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGATGT ATACGTGCTG ATGTTTCTGG CTGACACTGT AGACTTCATT
    ATCATCGTCT TTGGATTCTG GGCCTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCTCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTCCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTGGGCAA GGTCATCTTC
    CAGGTCATCC TCGTGTTTGG GATTCACTTC TGGATGTTCT TCATCTTACC CGGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTTTGGT ACTTTGTGAA GTGTGTGTAC
    TTTGGGCTGT CTGCCTATCA AATCCGCTGT GGCTACCCAA CCCGTGTGCT CGGGAACTTC
    CTCACAAAGA GCTACAATTA CGTCAACCTC TTCTTGTTTC AAGGGTTCCG CCTGGTCCCA
    TTTCTGACTG AGCTGCGGGC TGTGATGGAT TGGGTGTGGA CAGATACAAC CCTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCGCACATTT TTATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGTCAGAAGA AGAAGAAAGC CGTGAAGTAC
    GGCATGGGCG GCATGATCAT CGTCCTTCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAATCTGT CGCAGGGGTT ATCAACCAAC CCCTGGATGT GTCCGTCACA
    ATTACCCTGG GAGGTTATCA GCCTATCTTC ACAATGAGCG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATA ATTCAAAGTA TAATGAATTT CTAAAATCTT TCGGCCCCAA CAGTGGCGCA
    ATGCAATTTC TGGAGAACTA TGAAAGGGAA GACGTAACAG TAGCAGAACT GGAAGGGAAC
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTTTG
    GGTGCAAAAG CCGAAATAGC AACGGATAAG CTTTCTTTCC CTCTTGCGGT CGCAACACGG
    AATAGCATAG CTAAAATGAT TGCTGGCAAT GACACAGAAA GTTCAAACAC GCCAGTGACA
    ATAGAGAAGA TCTACCCATA CTATGTGAAG GCACCCAGCG ATTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCTGAAAA TAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CCGGAAGTAG GATATTCAAC
    CAGGGGTCCC AAGCCTTGGA ACTGGTGGTC TTCAATGACA AAGTCAGCCC TCCAAGTCTA
    GGCTTCTTGG CTGGCTATGG TATCATGGGA TTGTATGCAT CTGTTGTCCT TGTAATTGGG
    AAATTTGTTC GTGAGTTCTT CAGTGGGATC TCTCATTCCA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATCTTGAA GTTGTGCACA GATATATTCC TCGTGAGGGA GACAGGGGAG
    TTGGAACTGG AGGAAGACCT CTACGCCAAG TTGATATTCC TGTACAGGTC ACCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526231.1
    CDS1041..9533
    Translation

    Target ORF information:

    RefSeq Version XM_006526168.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526168.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACCGTGAA TGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGCCTG
    CCCATAGAGA ATGGCCCTGC CAAGACGGAC CTGTACACCA CTCCCCAGTA CCGGTGGGAG
    CCTTCGGAGG AGTCCTCAGA AAAGAAAGAG GAAGAAGAGG ACAAGAGAGA AGACTCCGAA
    GGGGAAGGGA GCCAGGAGGA GAAAAGGAGT GTGAGAATGC ACGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC TTTCGTGCTG TTGATCTGGT CCTGCACCCT CTGGATGATT
    CGCAACAGGA GGAAGTATGC CATGATCAGT TCTCCTTTCA TGGTCGTCTA TGCGAATCTG
    CTGCTGGTGC TGCAGTACAT ATGGAGCTTC GAGCTCCCCG AGATTAAGAA GGTCCCCGGG
    TTTCTAGAAA AGAAAGAGCC GGGAGAACTT GCTTCAAAGA TTCTCTTCAC CATCACGTTC
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TGAGGGAAAA GGAAGCACTT
    CTTTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGAAGAAA AGGAAGATGA GGAGCTCCAA
    GATGTACAAG TGGAAGGGGA GCCCACAGAG AAGGAAGAAG AAGAGGAAGA GGAAATAAAG
    GAAGAAAGGC ATGAGGTAAA GAAGGAAGAG GAAGAGGAAG TGGAGGAAGA TGATGACCAG
    GACATCATGA AGGTGCTGGG TAATCTGGTG GTGGCCCTCT TCATCAAGTA TTGGATCTAC
    GTCTGCGGGG GCATGTTCTT TTTTGTCAGC TTTGAGGGGA AAATCGTCAT GTACAAAATC
    ATCTACATGG TGCTGTTCCT GTTCTGCGTG GCCTTGTACC AGGTGCACTA CGAGTGGTGG
    AGGAAGATCC TGAAGTACTT CTGGATGTCG GTGGTCATTT ATACCATGTT GGTGCTCATC
    TTCATATACA CTTACCAGTT TGAAAACTTC CCAGGCCTGT GGCAAAACAT GACCGGATTA
    AAGAAAGAAA AGCTCGAGGA CCTCGGCCTG AAGCAGTTCA CGGTCGCTGA ACTGTTCACT
    CGCATATTCA TCCCCACCTC CTTTCTACTC GTCTGCATCC TACACCTCCA CTACTTCCAC
    GATCGGTTCC TGGAACTTAC AGACCTCAAG TCCATCCCCA GCAAAGAAGA CAACACCATC
    TACAGGTTGG CCCACCCTGA AGGAAGTCTT CCAGACTTAG CCATCATGAA TATGACGGCC
    AGCCTTGATA AGCCTGAGGT GCAGAAGTTG GCAGAGTCTG GGGAGGAGAG GCCAGAAGAG
    TGTGTCAAGA AGACGGAGAA GGGTGAGGCT GGGAAAGACA GTGATGAGTC TGAGGAGGAG
    GAAGATGAAG AAGAGGAATC CGAGGAAGAA GAAAGCTCAG ACCTCAGGAA CAAGTGGCAC
    CTGGTGATTG ACCGGCTCAC TGTGCTCTTT CTAAAATTCC TGGAGTATTT CCACAAGTTG
    CAGGTGTTTA TGTGGTGGAT CTTGGAGCTG CACATCATCA AAATCGTCTC ATCGTACATT
    ATCTGGGTGA CTGTGAAAGA GGTGTCTCTG TTCAACTATG TATTTTTGAT TTCTTGGGCA
    TTTGCTCTGC CATATGCCAA ACTCCGTCGT GCGGCTTCCA GTGTCTGCAC TGTCTGGACA
    TGTGTGATCA TCGTCTGCAA AATGTTGTAC CAGCTGCAAA CCATTAAACC TGAGAACTTT
    TCTGTCAACT GCTCCTTGCC CAATGAAAAT CAAACCAACA TTCCCCTTCA TGAGTTGAAC
    AAGTCTCTTC TCTACAGTGC CCCTGTTGAT CCCACTGAGT GGGTAGGCCT GAGGAAGTCC
    TCTCCTTTGC TCGTCTACCT GAGGAACAAC CTCCTGATGC TGGCGATTTT GGCCTTTGAA
    GTCACCGTTT ACCGCCATCA GGAGTACTAT CGAGGCCGAA ACAACCTGAC TGCTCCAGTG
    TCTAAAACTA TCTTTCATGA CATCACAAGG TTACATCTAG ATGATGGGCT TATCAATTGT
    GCCAAATATT TTGTAAATTA CTTCTTCTAC AAGTTTGGCC TAGAGACCTG TTTCCTAATG
    TCCGTGAATG TAATTGGACA GCGAATGGAC TTCTACGCCA TGATTCATGC CTGTTGGTTG
    ATCGGTGTCT TGTATCGACG CAGAAGAAAA GCCATTGCCG AGGTCTGGCC CAAGTACTGC
    TGCTTCCTGG CTTGCATCAT CACCTTCCAG TACTTCGTCT GCATCGGCAT CCCCCCTGCC
    CCTTGCCGAG ATTATCCATG GAGATTTAAG GGAGCCTACT TCAATGATAA CATTATCAAG
    TGGCTGTACT TCCCGGATTT CATTGTGCGG CCCAACCCCG TGTTTCTTGT CTATGACTTC
    ATGCTGCTCC TGTGTGCTTC CTTACAACGA CAGATCTTTG AGGACGAAAA CAAGGCAGCC
    GTGCGCATCA TGGCCGGGGA CAATGTCGAG ATCTGCATGA ACCTTGATGC AGCCTCGTTC
    AGCCAGCATA ACCCTGTGCC AGATTTCATT CACTGCAGAT CTTACTTGGA CATGTCCAAA
    GTGATCATCT TCAGCTACCT CTTCTGGTTC GTCCTCACCA TCATCTTCAT TACGGGAACA
    ACCAGGATCA GCATATTTTG CATGGGCTAC TTGGTGGCCT GTTTCTATTT CCTGCTCTTT
    GGAGGGGATC TGCTGCTGAA GCCCATCAAG AGCATCCTTC GTTACTGGGA CTGGCTGATT
    GCATACAACG TGTTTGTGAT CACCATGAAG AATATACTGT CAATAGGAGC CTGTGGATAC
    ATTGGCGCAC TAGTAAGAAA CAGCTGCTGG TTGATCCAAG CGTTCAGCCT GGCCTGCACC
    GTGAAAGGCT ATCAGATGCC TGAAGATGAC TCCAGATGCA AGCTGCCCAG TGGGGAGGCA
    GGGATCATCT GGGACAGCAT CTGCTTTGCG TTTCTGCTGC TGCAGAGGAG AGTCTTCATG
    AGCTACTACT TCCTGCATGT CGTGGCTGAC ATTAAAGCAT CCCAGATCCT GGCATCCAGA
    GGGGCCGAAC TTTTCCAGGC TACAATTGTG AAAGCTGTAA AGGCAAGAAT TGAAGAAGAG
    AAAAAATCCA TGGACCAGCT GAAGAGACAG ATGGATCGCA TCAAAGCCCG GCAACAAAAG
    TACAAAAAGG GTAAGGAGAG GATGCTGAGC TTGACCCAGG AGTCAGGGGA AGGCCAGGAC
    ATCCAGAAAG TCTCAGAAGA AGATGATGAA AGAGAAGCAG ACAAACAGAA AGCCAAGGGC
    AAAAAAAAGC AGTGGTGGCG GCCTTGGGTT GATCATGCTT CCATGGTCAG GAGTGGAGAT
    TATTATTTGT TTGAAACGGA TAGTGAAGAG GAGGAAGAAG AGGAACTTAA AAAGGAGGAT
    GAAGAACCCC CACGGAAGTC AGCATTCCAG AGAGCTATTG GGAAGTTTGC CTCAGCCATT
    TTAGCCTTGC CAAAGTCTGT CATTAAACTT CCCAAAACTA TTCTTCAGTA TTTAATCAGA
    GCTGCAAAGT TCGTCTACCA AGCCTGGATT ACTGATCCTA AAACGGCACT CCGACAGAGG
    AGGAAAGAGA AGAAAAAGTT GGCCAGAGAA GAGCAGAAGG AGAGACGCAA AGGATCTGGG
    GACGGTCCAG TGGAATGGGA AGATCGAGAG GATGAGCCAG TCAAAAAGAA ATCTGATGGA
    CCAGATAATA TCATCAAGAG GATATTTAAT ATCCTGAAGT TCACATGGGT TCTGTTCCTG
    GCCACAGTGG ATAGTTTCAC CACTTGGCTC AACTCCATCT CAAGGGAACA CATCGACATA
    TCCACAGTCC TGCGGATTGA AAGATGCATG CTGACTCGAG AGATAAAAAA GGGCAATGTT
    CCAACACGGG AAAGCATCCA CATGTACTAC CAGAACCACA TTATGAACCT TTCCCGAGAA
    TCCGGCCTGG ACACAATCGA CGAGCACTCG GGGGCTGGCT CCAGGGCCCA GGCAGCACAT
    AGGATGGATA GCTTAGATTC CCGGGACAGT ATCTCCAGCT GCTACACTGA AGCGACCCTG
    CTGATCTCAA GGCAGTCCAC CCTTGATGAT TTAGATGGAC AAGACCCCGT TCCTAAAACA
    AGCGAGCGTG CTCGGCCTAG GCTCCGTAAA ATGTTCAGCC TGGACATGTC CTCTTCGTCA
    GCTGACAGTG GCAGTGTAGC ATCAAGCGAG CCCACACAGT GTACCATGCT GTATTCGCGC
    CAAGGAACTA CGGAAACCAT CGAGGAGGTG GAGGCTGAAG CTGAGGAGGA GGTGGTAGAA
    GGTCTGGAGC CTGAGCTCCA TGATGCAGAA GAAAAGGAAT ACGCAGCTGA ATATGAAGCA
    GGGGTAGAGG AGATCAGCCT CACTCCAGAT GAGGAATTGC CTCAGTTCTC TACAGATGAC
    TGTGATGTGG AGGCGCCGCC CTCTTACAGC AAGGCTGTCA GCTTTGAGCA TCTATCGTTT
    GCGTCGCAAG ATGACTCTGG GGCCAAGAAC CACATGGTGG TCAGTCCTGA TGACAGCCGT
    ACAGACAAGC TGGAGTCAAG CATCTTACCA CCTCTGACTC ATGAGCTGAC AGCCAGCGAT
    CTGCTGATGA GCAAGATGTT CCATGATGAT GAGCTTGAGG AGTCTGAGAA GTTCTACGTG
    GACCAGCCCC GGTTTTTGCT GCTATTCTAT GCCATGTATA ACACCCTGGT GGCCCGCTCA
    GAAATGGTGT GCTATTTCGT GATTATCCTG AACCACATGA CCTCTGCCTC CATCATCACT
    CTCCTGTTGC CCATCTTGAT CTTCCTCTGG GCCATGCTGT CTGTTCCCAG GCCCAGCCGA
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TCGTAGTGAA ATACTTCTTC
    CAGTTCGGCT TCTTTCCCTG GAATAAGGAC TTGGAAATAT ACAAGGAAAG GCCCTACTTT
    CCACCGAACA TCATCGGGGT GGAGAAGAAG GAGGGCTATG TTCTTTATGA CCTCATTCAG
    CTGCTGGCTC TATTCTTCCA TCGCTCCATT CTGAAGTGTC ATGGTCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCGGATG ACGAGCTGTC CCTCGATCAG
    GGCAGAAGAG GCTCCTCAGA CTCACTGAAG TCTATCAACC TGGCTGCCTC TGTAGAGTCT
    GTCCACGTGA CCTTCCCCGA GCAGCCAGCG GCCATCAGGA GAAAACGCTC CTGCAGTAGC
    TCTCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGGAACA GTAGCCAGAA AGGAAGCAGT GTTTTAAGCC TTAAACAGAA AAGCAAACGA
    GAACTTTACA TGGAAAAGCT TCAAGAGCAT TTAATCAAAG CAAAGGCGTT TACCATAAAG
    AAGACTCTAC AGATCTATGT CCCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGATGT ATACGTGCTG ATGTTTCTGG CTGACACTGT AGACTTCATT
    ATCATCGTCT TTGGATTCTG GGCCTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCTCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTCCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTGGGCAA GGTCATCTTC
    CAGGTCATCC TCGTGTTTGG GATTCACTTC TGGATGTTCT TCATCTTACC CGGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTTTGGT ACTTTGTGAA GTGTGTGTAC
    TTTGGGCTGT CTGCCTATCA AATCCGCTGT GGCTACCCAA CCCGTGTGCT CGGGAACTTC
    CTCACAAAGA GCTACAATTA CGTCAACCTC TTCTTGTTTC AAGGGTTCCG CCTGGTCCCA
    TTTCTGACTG AGCTGCGGGC TGTGATGGAT TGGGTGTGGA CAGATACAAC CCTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCGCACATTT TTATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGTCAGAAGA AGAAGAAAGC CGTGAAGTAC
    GGCATGGGCG GCATGATCAT CGTCCTTCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAATCTGT CGCAGGGGTT ATCAACCAAC CCCTGGATGT GTCCGTCACA
    ATTACCCTGG GAGGTTATCA GCCTATCTTC ACAATGAGCG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATA ATTCAAAGTA TAATGAATTT CTAAAATCTT TCGGCCCCAA CAGTGGCGCA
    ATGCAATTTC TGGAGAACTA TGAAAGGGAA GACGTAACAG TAGCAGAACT GGAAGGGAAC
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTTTG
    GGTGCAAAAG CCGAAATAGC AACGGATAAG CTTTCTTTCC CTCTTGCGGT CGCAACACGG
    AATAGCATAG CTAAAATGAT TGCTGGCAAT GACACAGAAA GTTCAAACAC GCCAGTGACA
    ATAGAGAAGA TCTACCCATA CTATGTGAAG GCACCCAGCG ATTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCTGAAAA TAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CCGGAAGTAG GATATTCAAC
    CAGGGGTCCC AAGCCTTGGA ACTGGTGGTC TTCAATGACA AAGTCAGCCC TCCAAGTCTA
    GGCTTCTTGG CTGGCTATGG TATCATGGGA TTGTATGCAT CTGTTGTCCT TGTAATTGGG
    AAATTTGTTC GTGAGTTCTT CAGTGGGATC TCTCATTCCA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATCTTGAA GTTGTGCACA GATATATTCC TCGTGAGGGA GACAGGGGAG
    TTGGAACTGG AGGAAGACCT CTACGCCAAG TTGATATTCC TGTACAGGTC ACCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54735
    Clone ID Related Accession (Same CDS sequence) XM_006526169.3 , XM_006526169.4
    Accession Version XM_006526169.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACCGTGAA TGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGCCTG
    CCCATAGAGA ATGGCCCTGC CAAGACGGAC CTGTACACCA CTCCCCAGTA CCGGTGGGAG
    CCTTCGGAGG AGTCCTCAGA AAAGAAAGAG GAAGAAGAGG ACAAGAGAGA AGACTCCGAA
    GGGGAAGGGA GCCAGGAGGA GAAAAGGAGT GTGAGAATGC ACGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC TTTCGTGCTG TTGATCTGGT CCTGCACCCT CTGGATGATT
    CGCAACAGGA GGAAGTATGC CATGATCAGT TCTCCTTTCA TGGTCGTCTA TGCGAATCTG
    CTGCTGGTGC TGCAGTACAT ATGGAGCTTC GAGCTCCCCG AGATTAAGAA GGTCCCCGGG
    TTTCTAGAAA AGAAAGAGCC GGGAGAACTT GCTTCAAAGA TTCTCTTCAC CATCACGTTC
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TGAGGGAAAA GGAAGCACTT
    CTTTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGAAGAAA AGGATGAGGA GCTCCAAGAT
    GTACAAGTGG AAGGGGAGCC CACAGAGAAG GAAGAAGAAG AGGAAGAGGA AATAAAGGAA
    GAAAGGCATG AGGTAAAGAA GGAAGAGGAA GAGGAAGTGG AGGAAGATGA TGACCAGGAC
    ATCATGAAGG TGCTGGGTAA TCTGGTGGTG GCCCTCTTCA TCAAGTATTG GATCTACGTC
    TGCGGGGGCA TGTTCTTTTT TGTCAGCTTT GAGGGGAAAA TCGTCATGTA CAAAATCATC
    TACATGGTGC TGTTCCTGTT CTGCGTGGCC TTGTACCAGG TGCACTACGA GTGGTGGAGG
    AAGATCCTGA AGTACTTCTG GATGTCGGTG GTCATTTATA CCATGTTGGT GCTCATCTTC
    ATATACACTT ACCAGTTTGA AAACTTCCCA GGCCTGTGGC AAAACATGAC CGGATTAAAG
    AAAGAAAAGC TCGAGGACCT CGGCCTGAAG CAGTTCACGG TCGCTGAACT GTTCACTCGC
    ATATTCATCC CCACCTCCTT TCTACTCGTC TGCATCCTAC ACCTCCACTA CTTCCACGAT
    CGGTTCCTGG AACTTACAGA CCTCAAGTCC ATCCCCAGCA AAGAAGACAA CACCATCTAC
    AGGTTGGCCC ACCCTGAAGG AAGTCTTCCA GACTTAGCCA TCATGAATAT GACGGCCAGC
    CTTGATAAGC CTGAGGTGCA GAAGTTGGCA GAGTCTGGGG AGGAGAGGCC AGAAGAGTGT
    GTCAAGAAGA CGGAGAAGGG TGAGGCTGGG AAAGACAGTG ATGAGTCTGA GGAGGAGGAA
    GATGAAGAAG AGGAATCCGA GGAAGAAGAA AGCTCAGACC TCAGGAACAA GTGGCACCTG
    GTGATTGACC GGCTCACTGT GCTCTTTCTA AAATTCCTGG AGTATTTCCA CAAGTTGCAG
    GTGTTTATGT GGTGGATCTT GGAGCTGCAC ATCATCAAAA TCGTCTCATC GTACATTATC
    TGGGTGACTG TGAAAGAGGT GTCTCTGTTC AACTATGTAT TTTTGATTTC TTGGGCATTT
    GCTCTGCCAT ATGCCAAACT CCGTCGTGCG GCTTCCAGTG TCTGCACTGT CTGGACATGT
    GTGATCATCG TCTGCAAAAT GTTGTACCAG CTGCAAACCA TTAAACCTGA GAACTTTTCT
    GTCAACTGCT CCTTGCCCAA TGAAAATCAA ACCAACATTC CCCTTCATGA GTTGAACAAG
    TCTCTTCTCT ACAGTGCCCC TGTTGATCCC ACTGAGTGGG TAGGCCTGAG GAAGTCCTCT
    CCTTTGCTCG TCTACCTGAG GAACAACCTC CTGATGCTGG CGATTTTGGC CTTTGAAGTC
    ACCGTTTACC GCCATCAGGA GTACTATCGA GGCCGAAACA ACCTGACTGC TCCAGTGTCT
    AAAACTATCT TTCATGACAT CACAAGGTTA CATCTAGATG ATGGGCTTAT CAATTGTGCC
    AAATATTTTG TAAATTACTT CTTCTACAAG TTTGGCCTAG AGACCTGTTT CCTAATGTCC
    GTGAATGTAA TTGGACAGCG AATGGACTTC TACGCCATGA TTCATGCCTG TTGGTTGATC
    GGTGTCTTGT ATCGACGCAG AAGAAAAGCC ATTGCCGAGG TCTGGCCCAA GTACTGCTGC
    TTCCTGGCTT GCATCATCAC CTTCCAGTAC TTCGTCTGCA TCGGCATCCC CCCTGCCCCT
    TGCCGAGATT ATCCATGGAG ATTTAAGGGA GCCTACTTCA ATGATAACAT TATCAAGTGG
    CTGTACTTCC CGGATTTCAT TGTGCGGCCC AACCCCGTGT TTCTTGTCTA TGACTTCATG
    CTGCTCCTGT GTGCTTCCTT ACAACGACAG ATCTTTGAGG ACGAAAACAA GGCAGCCGTG
    CGCATCATGG CCGGGGACAA TGTCGAGATC TGCATGAACC TTGATGCAGC CTCGTTCAGC
    CAGCATAACC CTGTGCCAGA TTTCATTCAC TGCAGATCTT ACTTGGACAT GTCCAAAGTG
    ATCATCTTCA GCTACCTCTT CTGGTTCGTC CTCACCATCA TCTTCATTAC GGGAACAACC
    AGGATCAGCA TATTTTGCAT GGGCTACTTG GTGGCCTGTT TCTATTTCCT GCTCTTTGGA
    GGGGATCTGC TGCTGAAGCC CATCAAGAGC ATCCTTCGTT ACTGGGACTG GCTGATTGCA
    TACAACGTGT TTGTGATCAC CATGAAGAAT ATACTGTCAA TAGGAGCCTG TGGATACATT
    GGCGCACTAG TAAGAAACAG CTGCTGGTTG ATCCAAGCGT TCAGCCTGGC CTGCACCGTG
    AAAGGCTATC AGATGCCTGA AGATGACTCC AGATGCAAGC TGCCCAGTGG GGAGGCAGGG
    ATCATCTGGG ACAGCATCTG CTTTGCGTTT CTGCTGCTGC AGAGGAGAGT CTTCATGAGC
    TACTACTTCC TGCATGTCGT GGCTGACATT AAAGCATCCC AGATCCTGGC ATCCAGAGGG
    GCCGAACTTT TCCAGGCTAC AATTGTGAAA GCTGTAAAGG CAAGAATTGA AGAAGAGAAA
    AAATCCATGG ACCAGCTGAA GAGACAGATG GATCGCATCA AAGCCCGGCA ACAAAAGTAC
    AAAAAGGGTA AGGAGAGGAT GCTGAGCTTG ACCCAGGAGT CAGGGGAAGG CCAGGACATC
    CAGAAAGTCT CAGAAGAAGA TGATGAAAGA GAAGCAGACA AACAGAAAGC CAAGGGCAAA
    AAAAAGCAGT GGTGGCGGCC TTGGGTTGAT CATGCTTCCA TGGTCAGGAG TGGAGATTAT
    TATTTGTTTG AAACGGATAG TGAAGAGGAG GAAGAAGAGG AACTTAAAAA GGAGGATGAA
    GAACCCCCAC GGAAGTCAGC ATTCCAGAGA GCTATTGGGA AGTTTGCCTC AGCCATTTTA
    GCCTTGCCAA AGTCTGTCAT TAAACTTCCC AAAACTATTC TTCAGTATTT AATCAGAGCT
    GCAAAGTTCG TCTACCAAGC CTGGATTACT GATCCTAAAA CGGCACTCCG ACAGAGGAGG
    AAAGAGAAGA AAAAGTTGGC CAGAGAAGAG CAGAAGGAGA GACGCAAAGG ATCTGGGGAC
    GGTCCAGTGG AATGGGAAGA TCGAGAGGAT GAGCCAGTCA AAAAGAAATC TGATGGACCA
    GATAATATCA TCAAGAGGAT ATTTAATATC CTGAAGTTCA CATGGGTTCT GTTCCTGGCC
    ACAGTGGATA GTTTCACCAC TTGGCTCAAC TCCATCTCAA GGGAACACAT CGACATATCC
    ACAGTCCTGC GGATTGAAAG ATGCATGCTG ACTCGAGAGA TAAAAAAGGG CAATGTTCCA
    ACACGGGAAA GCATCCACAT GTACTACCAG AACCACATTA TGAACCTTTC CCGAGAATCC
    GGCCTGGACA CAATCGACGA GCACTCGGGG GCTGGCTCCA GGGCCCAGGC AGCACATAGG
    ATGGATAGCT TAGATTCCCG GGACAGTATC TCCAGCTGCT ACACTGAAGC GACCCTGCTG
    ATCTCAAGGC AGTCCACCCT TGATGATTTA GATGGACAAG ACCCCGTTCC TAAAACAAGC
    GAGCGTGCTC GGCCTAGGCT CCGTAAAATG TTCAGCCTGG ACATGTCCTC TTCGTCAGCT
    GACAGTGGCA GTGTAGCATC AAGCGAGCCC ACACAGTGTA CCATGCTGTA TTCGCGCCAA
    GGAACTACGG AAACCATCGA GGAGGTGGAG GCTGAAGCTG AGGAGGAGGT GGTAGAAGGT
    CTGGAGCCTG AGCTCCATGA TGCAGAAGAA AAGGAATACG CAGCTGAATA TGAAGCAGGG
    GTAGAGGAGA TCAGCCTCAC TCCAGATGAG GAATTGCCTC AGTTCTCTAC AGATGACTGT
    GATGTGGAGG CGCCGCCCTC TTACAGCAAG GCTGTCAGCT TTGAGCATCT ATCGTTTGCG
    TCGCAAGATG ACTCTGGGGC CAAGAACCAC ATGGTGGTCA GTCCTGATGA CAGCCGTACA
    GACAAGCTGG AGTCAAGCAT CTTACCACCT CTGACTCATG AGCTGACAGC CAGCGATCTG
    CTGATGAGCA AGATGTTCCA TGATGATGAG CTTGAGGAGT CTGAGAAGTT CTACGTGGAC
    CAGCCCCGGT TTTTGCTGCT ATTCTATGCC ATGTATAACA CCCTGGTGGC CCGCTCAGAA
    ATGGTGTGCT ATTTCGTGAT TATCCTGAAC CACATGACCT CTGCCTCCAT CATCACTCTC
    CTGTTGCCCA TCTTGATCTT CCTCTGGGCC ATGCTGTCTG TTCCCAGGCC CAGCCGACGC
    TTCTGGATGA TGGCCATCGT CTACACTGAG GTGGCGATCG TAGTGAAATA CTTCTTCCAG
    TTCGGCTTCT TTCCCTGGAA TAAGGACTTG GAAATATACA AGGAAAGGCC CTACTTTCCA
    CCGAACATCA TCGGGGTGGA GAAGAAGGAG GGCTATGTTC TTTATGACCT CATTCAGCTG
    CTGGCTCTAT TCTTCCATCG CTCCATTCTG AAGTGTCATG GTCTATGGGA CGAGGATGAC
    ATTGTTGACA GTAACACAGA CAAGGAAGGA TCGGATGACG AGCTGTCCCT CGATCAGGGC
    AGAAGAGGCT CCTCAGACTC ACTGAAGTCT ATCAACCTGG CTGCCTCTGT AGAGTCTGTC
    CACGTGACCT TCCCCGAGCA GCCAGCGGCC ATCAGGAGAA AACGCTCCTG CAGTAGCTCT
    CAGATCTCCC CACGGTCCAG CTTCTCTTCA AATAGGTCCA AAAGAGGCAG CACAAGCACC
    CGGAACAGTA GCCAGAAAGG AAGCAGTGTT TTAAGCCTTA AACAGAAAAG CAAACGAGAA
    CTTTACATGG AAAAGCTTCA AGAGCATTTA ATCAAAGCAA AGGCGTTTAC CATAAAGAAG
    ACTCTACAGA TCTATGTCCC CATTCGGCAA TTCTTCTATG ACCTCATCCA CCCAGACTAC
    AGTGCTGTGA CTGATGTATA CGTGCTGATG TTTCTGGCTG ACACTGTAGA CTTCATTATC
    ATCGTCTTTG GATTCTGGGC CTTTGGGAAA CACTCGGCAG CTGCAGATAT CACCTCTTCT
    CTATCAGAAG ACCAGGTCCC CGGGCCATTC CTGGTGATGG TCCTCATTCA GTTTGGAACC
    ATGGTGGTGG ACCGTGCACT CTACCTCAGG AAGACTGTGC TGGGCAAGGT CATCTTCCAG
    GTCATCCTCG TGTTTGGGAT TCACTTCTGG ATGTTCTTCA TCTTACCCGG TGTGACTGAG
    AGGAAATTCA GCCAGAACCT GGTGGCACAG CTTTGGTACT TTGTGAAGTG TGTGTACTTT
    GGGCTGTCTG CCTATCAAAT CCGCTGTGGC TACCCAACCC GTGTGCTCGG GAACTTCCTC
    ACAAAGAGCT ACAATTACGT CAACCTCTTC TTGTTTCAAG GGTTCCGCCT GGTCCCATTT
    CTGACTGAGC TGCGGGCTGT GATGGATTGG GTGTGGACAG ATACAACCCT GAGCCTCTCC
    AGCTGGATTT GTGTGGAGGA CATTTACGCG CACATTTTTA TCTTAAAGTG CTGGCGAGAG
    TCGGAAAAGA GATACCCTCA ACCCAGGGGT CAGAAGAAGA AGAAAGCCGT GAAGTACGGC
    ATGGGCGGCA TGATCATCGT CCTTCTCATC TGTATCGTCT GGTTTCCTCT GCTCTTCATG
    TCTTTGATCA AATCTGTCGC AGGGGTTATC AACCAACCCC TGGATGTGTC CGTCACAATT
    ACCCTGGGAG GTTATCAGCC TATCTTCACA ATGAGCGCCC AGCAAAGCCA GCTGAAAGTT
    ATGGATAATT CAAAGTATAA TGAATTTCTA AAATCTTTCG GCCCCAACAG TGGCGCAATG
    CAATTTCTGG AGAACTATGA AAGGGAAGAC GTAACAGTAG CAGAACTGGA AGGGAACTCG
    AATTCTTTGT GGACCATCAG CCCTCCCAGT AAGCAGAAAA TGATACAGGA GCTCACCGAC
    CCCAATAGTT GCTTCTCTGT TGTTTTTTCA TGGAGTATCC AGAGAAACAT GACTTTGGGT
    GCAAAAGCCG AAATAGCAAC GGATAAGCTT TCTTTCCCTC TTGCGGTCGC AACACGGAAT
    AGCATAGCTA AAATGATTGC TGGCAATGAC ACAGAAAGTT CAAACACGCC AGTGACAATA
    GAGAAGATCT ACCCATACTA TGTGAAGGCA CCCAGCGATT CAAACTCAAA GCCTATAAAA
    CAGCTTCTGT CTGAAAATAA TTTCATGAAT ATCACCATCA TTTTGTTCAG AGACAATGTC
    ACAAAGTCCA ATAGTGAGTG GTGGGTTCTC AACCTGACCG GAAGTAGGAT ATTCAACCAG
    GGGTCCCAAG CCTTGGAACT GGTGGTCTTC AATGACAAAG TCAGCCCTCC AAGTCTAGGC
    TTCTTGGCTG GCTATGGTAT CATGGGATTG TATGCATCTG TTGTCCTTGT AATTGGGAAA
    TTTGTTCGTG AGTTCTTCAG TGGGATCTCT CATTCCATCA TGTTTGAAGA GCTTCCAAAT
    GTGGACAGAA TCTTGAAGTT GTGCACAGAT ATATTCCTCG TGAGGGAGAC AGGGGAGTTG
    GAACTGGAGG AAGACCTCTA CGCCAAGTTG ATATTCCTGT ACAGGTCACC AGAAACCATG
    ATCAAGTGGA CTAGAGAAAA AACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526232.1
    CDS1041..9530
    Translation

    Target ORF information:

    RefSeq Version XM_006526169.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526169.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACCGTGAA TGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGCCTG
    CCCATAGAGA ATGGCCCTGC CAAGACGGAC CTGTACACCA CTCCCCAGTA CCGGTGGGAG
    CCTTCGGAGG AGTCCTCAGA AAAGAAAGAG GAAGAAGAGG ACAAGAGAGA AGACTCCGAA
    GGGGAAGGGA GCCAGGAGGA GAAAAGGAGT GTGAGAATGC ACGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC TTTCGTGCTG TTGATCTGGT CCTGCACCCT CTGGATGATT
    CGCAACAGGA GGAAGTATGC CATGATCAGT TCTCCTTTCA TGGTCGTCTA TGCGAATCTG
    CTGCTGGTGC TGCAGTACAT ATGGAGCTTC GAGCTCCCCG AGATTAAGAA GGTCCCCGGG
    TTTCTAGAAA AGAAAGAGCC GGGAGAACTT GCTTCAAAGA TTCTCTTCAC CATCACGTTC
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TGAGGGAAAA GGAAGCACTT
    CTTTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGAAGAAA AGGATGAGGA GCTCCAAGAT
    GTACAAGTGG AAGGGGAGCC CACAGAGAAG GAAGAAGAAG AGGAAGAGGA AATAAAGGAA
    GAAAGGCATG AGGTAAAGAA GGAAGAGGAA GAGGAAGTGG AGGAAGATGA TGACCAGGAC
    ATCATGAAGG TGCTGGGTAA TCTGGTGGTG GCCCTCTTCA TCAAGTATTG GATCTACGTC
    TGCGGGGGCA TGTTCTTTTT TGTCAGCTTT GAGGGGAAAA TCGTCATGTA CAAAATCATC
    TACATGGTGC TGTTCCTGTT CTGCGTGGCC TTGTACCAGG TGCACTACGA GTGGTGGAGG
    AAGATCCTGA AGTACTTCTG GATGTCGGTG GTCATTTATA CCATGTTGGT GCTCATCTTC
    ATATACACTT ACCAGTTTGA AAACTTCCCA GGCCTGTGGC AAAACATGAC CGGATTAAAG
    AAAGAAAAGC TCGAGGACCT CGGCCTGAAG CAGTTCACGG TCGCTGAACT GTTCACTCGC
    ATATTCATCC CCACCTCCTT TCTACTCGTC TGCATCCTAC ACCTCCACTA CTTCCACGAT
    CGGTTCCTGG AACTTACAGA CCTCAAGTCC ATCCCCAGCA AAGAAGACAA CACCATCTAC
    AGGTTGGCCC ACCCTGAAGG AAGTCTTCCA GACTTAGCCA TCATGAATAT GACGGCCAGC
    CTTGATAAGC CTGAGGTGCA GAAGTTGGCA GAGTCTGGGG AGGAGAGGCC AGAAGAGTGT
    GTCAAGAAGA CGGAGAAGGG TGAGGCTGGG AAAGACAGTG ATGAGTCTGA GGAGGAGGAA
    GATGAAGAAG AGGAATCCGA GGAAGAAGAA AGCTCAGACC TCAGGAACAA GTGGCACCTG
    GTGATTGACC GGCTCACTGT GCTCTTTCTA AAATTCCTGG AGTATTTCCA CAAGTTGCAG
    GTGTTTATGT GGTGGATCTT GGAGCTGCAC ATCATCAAAA TCGTCTCATC GTACATTATC
    TGGGTGACTG TGAAAGAGGT GTCTCTGTTC AACTATGTAT TTTTGATTTC TTGGGCATTT
    GCTCTGCCAT ATGCCAAACT CCGTCGTGCG GCTTCCAGTG TCTGCACTGT CTGGACATGT
    GTGATCATCG TCTGCAAAAT GTTGTACCAG CTGCAAACCA TTAAACCTGA GAACTTTTCT
    GTCAACTGCT CCTTGCCCAA TGAAAATCAA ACCAACATTC CCCTTCATGA GTTGAACAAG
    TCTCTTCTCT ACAGTGCCCC TGTTGATCCC ACTGAGTGGG TAGGCCTGAG GAAGTCCTCT
    CCTTTGCTCG TCTACCTGAG GAACAACCTC CTGATGCTGG CGATTTTGGC CTTTGAAGTC
    ACCGTTTACC GCCATCAGGA GTACTATCGA GGCCGAAACA ACCTGACTGC TCCAGTGTCT
    AAAACTATCT TTCATGACAT CACAAGGTTA CATCTAGATG ATGGGCTTAT CAATTGTGCC
    AAATATTTTG TAAATTACTT CTTCTACAAG TTTGGCCTAG AGACCTGTTT CCTAATGTCC
    GTGAATGTAA TTGGACAGCG AATGGACTTC TACGCCATGA TTCATGCCTG TTGGTTGATC
    GGTGTCTTGT ATCGACGCAG AAGAAAAGCC ATTGCCGAGG TCTGGCCCAA GTACTGCTGC
    TTCCTGGCTT GCATCATCAC CTTCCAGTAC TTCGTCTGCA TCGGCATCCC CCCTGCCCCT
    TGCCGAGATT ATCCATGGAG ATTTAAGGGA GCCTACTTCA ATGATAACAT TATCAAGTGG
    CTGTACTTCC CGGATTTCAT TGTGCGGCCC AACCCCGTGT TTCTTGTCTA TGACTTCATG
    CTGCTCCTGT GTGCTTCCTT ACAACGACAG ATCTTTGAGG ACGAAAACAA GGCAGCCGTG
    CGCATCATGG CCGGGGACAA TGTCGAGATC TGCATGAACC TTGATGCAGC CTCGTTCAGC
    CAGCATAACC CTGTGCCAGA TTTCATTCAC TGCAGATCTT ACTTGGACAT GTCCAAAGTG
    ATCATCTTCA GCTACCTCTT CTGGTTCGTC CTCACCATCA TCTTCATTAC GGGAACAACC
    AGGATCAGCA TATTTTGCAT GGGCTACTTG GTGGCCTGTT TCTATTTCCT GCTCTTTGGA
    GGGGATCTGC TGCTGAAGCC CATCAAGAGC ATCCTTCGTT ACTGGGACTG GCTGATTGCA
    TACAACGTGT TTGTGATCAC CATGAAGAAT ATACTGTCAA TAGGAGCCTG TGGATACATT
    GGCGCACTAG TAAGAAACAG CTGCTGGTTG ATCCAAGCGT TCAGCCTGGC CTGCACCGTG
    AAAGGCTATC AGATGCCTGA AGATGACTCC AGATGCAAGC TGCCCAGTGG GGAGGCAGGG
    ATCATCTGGG ACAGCATCTG CTTTGCGTTT CTGCTGCTGC AGAGGAGAGT CTTCATGAGC
    TACTACTTCC TGCATGTCGT GGCTGACATT AAAGCATCCC AGATCCTGGC ATCCAGAGGG
    GCCGAACTTT TCCAGGCTAC AATTGTGAAA GCTGTAAAGG CAAGAATTGA AGAAGAGAAA
    AAATCCATGG ACCAGCTGAA GAGACAGATG GATCGCATCA AAGCCCGGCA ACAAAAGTAC
    AAAAAGGGTA AGGAGAGGAT GCTGAGCTTG ACCCAGGAGT CAGGGGAAGG CCAGGACATC
    CAGAAAGTCT CAGAAGAAGA TGATGAAAGA GAAGCAGACA AACAGAAAGC CAAGGGCAAA
    AAAAAGCAGT GGTGGCGGCC TTGGGTTGAT CATGCTTCCA TGGTCAGGAG TGGAGATTAT
    TATTTGTTTG AAACGGATAG TGAAGAGGAG GAAGAAGAGG AACTTAAAAA GGAGGATGAA
    GAACCCCCAC GGAAGTCAGC ATTCCAGAGA GCTATTGGGA AGTTTGCCTC AGCCATTTTA
    GCCTTGCCAA AGTCTGTCAT TAAACTTCCC AAAACTATTC TTCAGTATTT AATCAGAGCT
    GCAAAGTTCG TCTACCAAGC CTGGATTACT GATCCTAAAA CGGCACTCCG ACAGAGGAGG
    AAAGAGAAGA AAAAGTTGGC CAGAGAAGAG CAGAAGGAGA GACGCAAAGG ATCTGGGGAC
    GGTCCAGTGG AATGGGAAGA TCGAGAGGAT GAGCCAGTCA AAAAGAAATC TGATGGACCA
    GATAATATCA TCAAGAGGAT ATTTAATATC CTGAAGTTCA CATGGGTTCT GTTCCTGGCC
    ACAGTGGATA GTTTCACCAC TTGGCTCAAC TCCATCTCAA GGGAACACAT CGACATATCC
    ACAGTCCTGC GGATTGAAAG ATGCATGCTG ACTCGAGAGA TAAAAAAGGG CAATGTTCCA
    ACACGGGAAA GCATCCACAT GTACTACCAG AACCACATTA TGAACCTTTC CCGAGAATCC
    GGCCTGGACA CAATCGACGA GCACTCGGGG GCTGGCTCCA GGGCCCAGGC AGCACATAGG
    ATGGATAGCT TAGATTCCCG GGACAGTATC TCCAGCTGCT ACACTGAAGC GACCCTGCTG
    ATCTCAAGGC AGTCCACCCT TGATGATTTA GATGGACAAG ACCCCGTTCC TAAAACAAGC
    GAGCGTGCTC GGCCTAGGCT CCGTAAAATG TTCAGCCTGG ACATGTCCTC TTCGTCAGCT
    GACAGTGGCA GTGTAGCATC AAGCGAGCCC ACACAGTGTA CCATGCTGTA TTCGCGCCAA
    GGAACTACGG AAACCATCGA GGAGGTGGAG GCTGAAGCTG AGGAGGAGGT GGTAGAAGGT
    CTGGAGCCTG AGCTCCATGA TGCAGAAGAA AAGGAATACG CAGCTGAATA TGAAGCAGGG
    GTAGAGGAGA TCAGCCTCAC TCCAGATGAG GAATTGCCTC AGTTCTCTAC AGATGACTGT
    GATGTGGAGG CGCCGCCCTC TTACAGCAAG GCTGTCAGCT TTGAGCATCT ATCGTTTGCG
    TCGCAAGATG ACTCTGGGGC CAAGAACCAC ATGGTGGTCA GTCCTGATGA CAGCCGTACA
    GACAAGCTGG AGTCAAGCAT CTTACCACCT CTGACTCATG AGCTGACAGC CAGCGATCTG
    CTGATGAGCA AGATGTTCCA TGATGATGAG CTTGAGGAGT CTGAGAAGTT CTACGTGGAC
    CAGCCCCGGT TTTTGCTGCT ATTCTATGCC ATGTATAACA CCCTGGTGGC CCGCTCAGAA
    ATGGTGTGCT ATTTCGTGAT TATCCTGAAC CACATGACCT CTGCCTCCAT CATCACTCTC
    CTGTTGCCCA TCTTGATCTT CCTCTGGGCC ATGCTGTCTG TTCCCAGGCC CAGCCGACGC
    TTCTGGATGA TGGCCATCGT CTACACTGAG GTGGCGATCG TAGTGAAATA CTTCTTCCAG
    TTCGGCTTCT TTCCCTGGAA TAAGGACTTG GAAATATACA AGGAAAGGCC CTACTTTCCA
    CCGAACATCA TCGGGGTGGA GAAGAAGGAG GGCTATGTTC TTTATGACCT CATTCAGCTG
    CTGGCTCTAT TCTTCCATCG CTCCATTCTG AAGTGTCATG GTCTATGGGA CGAGGATGAC
    ATTGTTGACA GTAACACAGA CAAGGAAGGA TCGGATGACG AGCTGTCCCT CGATCAGGGC
    AGAAGAGGCT CCTCAGACTC ACTGAAGTCT ATCAACCTGG CTGCCTCTGT AGAGTCTGTC
    CACGTGACCT TCCCCGAGCA GCCAGCGGCC ATCAGGAGAA AACGCTCCTG CAGTAGCTCT
    CAGATCTCCC CACGGTCCAG CTTCTCTTCA AATAGGTCCA AAAGAGGCAG CACAAGCACC
    CGGAACAGTA GCCAGAAAGG AAGCAGTGTT TTAAGCCTTA AACAGAAAAG CAAACGAGAA
    CTTTACATGG AAAAGCTTCA AGAGCATTTA ATCAAAGCAA AGGCGTTTAC CATAAAGAAG
    ACTCTACAGA TCTATGTCCC CATTCGGCAA TTCTTCTATG ACCTCATCCA CCCAGACTAC
    AGTGCTGTGA CTGATGTATA CGTGCTGATG TTTCTGGCTG ACACTGTAGA CTTCATTATC
    ATCGTCTTTG GATTCTGGGC CTTTGGGAAA CACTCGGCAG CTGCAGATAT CACCTCTTCT
    CTATCAGAAG ACCAGGTCCC CGGGCCATTC CTGGTGATGG TCCTCATTCA GTTTGGAACC
    ATGGTGGTGG ACCGTGCACT CTACCTCAGG AAGACTGTGC TGGGCAAGGT CATCTTCCAG
    GTCATCCTCG TGTTTGGGAT TCACTTCTGG ATGTTCTTCA TCTTACCCGG TGTGACTGAG
    AGGAAATTCA GCCAGAACCT GGTGGCACAG CTTTGGTACT TTGTGAAGTG TGTGTACTTT
    GGGCTGTCTG CCTATCAAAT CCGCTGTGGC TACCCAACCC GTGTGCTCGG GAACTTCCTC
    ACAAAGAGCT ACAATTACGT CAACCTCTTC TTGTTTCAAG GGTTCCGCCT GGTCCCATTT
    CTGACTGAGC TGCGGGCTGT GATGGATTGG GTGTGGACAG ATACAACCCT GAGCCTCTCC
    AGCTGGATTT GTGTGGAGGA CATTTACGCG CACATTTTTA TCTTAAAGTG CTGGCGAGAG
    TCGGAAAAGA GATACCCTCA ACCCAGGGGT CAGAAGAAGA AGAAAGCCGT GAAGTACGGC
    ATGGGCGGCA TGATCATCGT CCTTCTCATC TGTATCGTCT GGTTTCCTCT GCTCTTCATG
    TCTTTGATCA AATCTGTCGC AGGGGTTATC AACCAACCCC TGGATGTGTC CGTCACAATT
    ACCCTGGGAG GTTATCAGCC TATCTTCACA ATGAGCGCCC AGCAAAGCCA GCTGAAAGTT
    ATGGATAATT CAAAGTATAA TGAATTTCTA AAATCTTTCG GCCCCAACAG TGGCGCAATG
    CAATTTCTGG AGAACTATGA AAGGGAAGAC GTAACAGTAG CAGAACTGGA AGGGAACTCG
    AATTCTTTGT GGACCATCAG CCCTCCCAGT AAGCAGAAAA TGATACAGGA GCTCACCGAC
    CCCAATAGTT GCTTCTCTGT TGTTTTTTCA TGGAGTATCC AGAGAAACAT GACTTTGGGT
    GCAAAAGCCG AAATAGCAAC GGATAAGCTT TCTTTCCCTC TTGCGGTCGC AACACGGAAT
    AGCATAGCTA AAATGATTGC TGGCAATGAC ACAGAAAGTT CAAACACGCC AGTGACAATA
    GAGAAGATCT ACCCATACTA TGTGAAGGCA CCCAGCGATT CAAACTCAAA GCCTATAAAA
    CAGCTTCTGT CTGAAAATAA TTTCATGAAT ATCACCATCA TTTTGTTCAG AGACAATGTC
    ACAAAGTCCA ATAGTGAGTG GTGGGTTCTC AACCTGACCG GAAGTAGGAT ATTCAACCAG
    GGGTCCCAAG CCTTGGAACT GGTGGTCTTC AATGACAAAG TCAGCCCTCC AAGTCTAGGC
    TTCTTGGCTG GCTATGGTAT CATGGGATTG TATGCATCTG TTGTCCTTGT AATTGGGAAA
    TTTGTTCGTG AGTTCTTCAG TGGGATCTCT CATTCCATCA TGTTTGAAGA GCTTCCAAAT
    GTGGACAGAA TCTTGAAGTT GTGCACAGAT ATATTCCTCG TGAGGGAGAC AGGGGAGTTG
    GAACTGGAGG AAGACCTCTA CGCCAAGTTG ATATTCCTGT ACAGGTCACC AGAAACCATG
    ATCAAGTGGA CTAGAGAAAA AACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54735
    Clone ID Related Accession (Same CDS sequence) XM_006526169.3 , XM_006526169.4
    Accession Version XM_006526169.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526169.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACCGTGAA TGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGCCTG
    CCCATAGAGA ATGGCCCTGC CAAGACGGAC CTGTACACCA CTCCCCAGTA CCGGTGGGAG
    CCTTCGGAGG AGTCCTCAGA AAAGAAAGAG GAAGAAGAGG ACAAGAGAGA AGACTCCGAA
    GGGGAAGGGA GCCAGGAGGA GAAAAGGAGT GTGAGAATGC ACGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC TTTCGTGCTG TTGATCTGGT CCTGCACCCT CTGGATGATT
    CGCAACAGGA GGAAGTATGC CATGATCAGT TCTCCTTTCA TGGTCGTCTA TGCGAATCTG
    CTGCTGGTGC TGCAGTACAT ATGGAGCTTC GAGCTCCCCG AGATTAAGAA GGTCCCCGGG
    TTTCTAGAAA AGAAAGAGCC GGGAGAACTT GCTTCAAAGA TTCTCTTCAC CATCACGTTC
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TGAGGGAAAA GGAAGCACTT
    CTTTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGAAGAAA AGGATGAGGA GCTCCAAGAT
    GTACAAGTGG AAGGGGAGCC CACAGAGAAG GAAGAAGAAG AGGAAGAGGA AATAAAGGAA
    GAAAGGCATG AGGTAAAGAA GGAAGAGGAA GAGGAAGTGG AGGAAGATGA TGACCAGGAC
    ATCATGAAGG TGCTGGGTAA TCTGGTGGTG GCCCTCTTCA TCAAGTATTG GATCTACGTC
    TGCGGGGGCA TGTTCTTTTT TGTCAGCTTT GAGGGGAAAA TCGTCATGTA CAAAATCATC
    TACATGGTGC TGTTCCTGTT CTGCGTGGCC TTGTACCAGG TGCACTACGA GTGGTGGAGG
    AAGATCCTGA AGTACTTCTG GATGTCGGTG GTCATTTATA CCATGTTGGT GCTCATCTTC
    ATATACACTT ACCAGTTTGA AAACTTCCCA GGCCTGTGGC AAAACATGAC CGGATTAAAG
    AAAGAAAAGC TCGAGGACCT CGGCCTGAAG CAGTTCACGG TCGCTGAACT GTTCACTCGC
    ATATTCATCC CCACCTCCTT TCTACTCGTC TGCATCCTAC ACCTCCACTA CTTCCACGAT
    CGGTTCCTGG AACTTACAGA CCTCAAGTCC ATCCCCAGCA AAGAAGACAA CACCATCTAC
    AGGTTGGCCC ACCCTGAAGG AAGTCTTCCA GACTTAGCCA TCATGAATAT GACGGCCAGC
    CTTGATAAGC CTGAGGTGCA GAAGTTGGCA GAGTCTGGGG AGGAGAGGCC AGAAGAGTGT
    GTCAAGAAGA CGGAGAAGGG TGAGGCTGGG AAAGACAGTG ATGAGTCTGA GGAGGAGGAA
    GATGAAGAAG AGGAATCCGA GGAAGAAGAA AGCTCAGACC TCAGGAACAA GTGGCACCTG
    GTGATTGACC GGCTCACTGT GCTCTTTCTA AAATTCCTGG AGTATTTCCA CAAGTTGCAG
    GTGTTTATGT GGTGGATCTT GGAGCTGCAC ATCATCAAAA TCGTCTCATC GTACATTATC
    TGGGTGACTG TGAAAGAGGT GTCTCTGTTC AACTATGTAT TTTTGATTTC TTGGGCATTT
    GCTCTGCCAT ATGCCAAACT CCGTCGTGCG GCTTCCAGTG TCTGCACTGT CTGGACATGT
    GTGATCATCG TCTGCAAAAT GTTGTACCAG CTGCAAACCA TTAAACCTGA GAACTTTTCT
    GTCAACTGCT CCTTGCCCAA TGAAAATCAA ACCAACATTC CCCTTCATGA GTTGAACAAG
    TCTCTTCTCT ACAGTGCCCC TGTTGATCCC ACTGAGTGGG TAGGCCTGAG GAAGTCCTCT
    CCTTTGCTCG TCTACCTGAG GAACAACCTC CTGATGCTGG CGATTTTGGC CTTTGAAGTC
    ACCGTTTACC GCCATCAGGA GTACTATCGA GGCCGAAACA ACCTGACTGC TCCAGTGTCT
    AAAACTATCT TTCATGACAT CACAAGGTTA CATCTAGATG ATGGGCTTAT CAATTGTGCC
    AAATATTTTG TAAATTACTT CTTCTACAAG TTTGGCCTAG AGACCTGTTT CCTAATGTCC
    GTGAATGTAA TTGGACAGCG AATGGACTTC TACGCCATGA TTCATGCCTG TTGGTTGATC
    GGTGTCTTGT ATCGACGCAG AAGAAAAGCC ATTGCCGAGG TCTGGCCCAA GTACTGCTGC
    TTCCTGGCTT GCATCATCAC CTTCCAGTAC TTCGTCTGCA TCGGCATCCC CCCTGCCCCT
    TGCCGAGATT ATCCATGGAG ATTTAAGGGA GCCTACTTCA ATGATAACAT TATCAAGTGG
    CTGTACTTCC CGGATTTCAT TGTGCGGCCC AACCCCGTGT TTCTTGTCTA TGACTTCATG
    CTGCTCCTGT GTGCTTCCTT ACAACGACAG ATCTTTGAGG ACGAAAACAA GGCAGCCGTG
    CGCATCATGG CCGGGGACAA TGTCGAGATC TGCATGAACC TTGATGCAGC CTCGTTCAGC
    CAGCATAACC CTGTGCCAGA TTTCATTCAC TGCAGATCTT ACTTGGACAT GTCCAAAGTG
    ATCATCTTCA GCTACCTCTT CTGGTTCGTC CTCACCATCA TCTTCATTAC GGGAACAACC
    AGGATCAGCA TATTTTGCAT GGGCTACTTG GTGGCCTGTT TCTATTTCCT GCTCTTTGGA
    GGGGATCTGC TGCTGAAGCC CATCAAGAGC ATCCTTCGTT ACTGGGACTG GCTGATTGCA
    TACAACGTGT TTGTGATCAC CATGAAGAAT ATACTGTCAA TAGGAGCCTG TGGATACATT
    GGCGCACTAG TAAGAAACAG CTGCTGGTTG ATCCAAGCGT TCAGCCTGGC CTGCACCGTG
    AAAGGCTATC AGATGCCTGA AGATGACTCC AGATGCAAGC TGCCCAGTGG GGAGGCAGGG
    ATCATCTGGG ACAGCATCTG CTTTGCGTTT CTGCTGCTGC AGAGGAGAGT CTTCATGAGC
    TACTACTTCC TGCATGTCGT GGCTGACATT AAAGCATCCC AGATCCTGGC ATCCAGAGGG
    GCCGAACTTT TCCAGGCTAC AATTGTGAAA GCTGTAAAGG CAAGAATTGA AGAAGAGAAA
    AAATCCATGG ACCAGCTGAA GAGACAGATG GATCGCATCA AAGCCCGGCA ACAAAAGTAC
    AAAAAGGGTA AGGAGAGGAT GCTGAGCTTG ACCCAGGAGT CAGGGGAAGG CCAGGACATC
    CAGAAAGTCT CAGAAGAAGA TGATGAAAGA GAAGCAGACA AACAGAAAGC CAAGGGCAAA
    AAAAAGCAGT GGTGGCGGCC TTGGGTTGAT CATGCTTCCA TGGTCAGGAG TGGAGATTAT
    TATTTGTTTG AAACGGATAG TGAAGAGGAG GAAGAAGAGG AACTTAAAAA GGAGGATGAA
    GAACCCCCAC GGAAGTCAGC ATTCCAGAGA GCTATTGGGA AGTTTGCCTC AGCCATTTTA
    GCCTTGCCAA AGTCTGTCAT TAAACTTCCC AAAACTATTC TTCAGTATTT AATCAGAGCT
    GCAAAGTTCG TCTACCAAGC CTGGATTACT GATCCTAAAA CGGCACTCCG ACAGAGGAGG
    AAAGAGAAGA AAAAGTTGGC CAGAGAAGAG CAGAAGGAGA GACGCAAAGG ATCTGGGGAC
    GGTCCAGTGG AATGGGAAGA TCGAGAGGAT GAGCCAGTCA AAAAGAAATC TGATGGACCA
    GATAATATCA TCAAGAGGAT ATTTAATATC CTGAAGTTCA CATGGGTTCT GTTCCTGGCC
    ACAGTGGATA GTTTCACCAC TTGGCTCAAC TCCATCTCAA GGGAACACAT CGACATATCC
    ACAGTCCTGC GGATTGAAAG ATGCATGCTG ACTCGAGAGA TAAAAAAGGG CAATGTTCCA
    ACACGGGAAA GCATCCACAT GTACTACCAG AACCACATTA TGAACCTTTC CCGAGAATCC
    GGCCTGGACA CAATCGACGA GCACTCGGGG GCTGGCTCCA GGGCCCAGGC AGCACATAGG
    ATGGATAGCT TAGATTCCCG GGACAGTATC TCCAGCTGCT ACACTGAAGC GACCCTGCTG
    ATCTCAAGGC AGTCCACCCT TGATGATTTA GATGGACAAG ACCCCGTTCC TAAAACAAGC
    GAGCGTGCTC GGCCTAGGCT CCGTAAAATG TTCAGCCTGG ACATGTCCTC TTCGTCAGCT
    GACAGTGGCA GTGTAGCATC AAGCGAGCCC ACACAGTGTA CCATGCTGTA TTCGCGCCAA
    GGAACTACGG AAACCATCGA GGAGGTGGAG GCTGAAGCTG AGGAGGAGGT GGTAGAAGGT
    CTGGAGCCTG AGCTCCATGA TGCAGAAGAA AAGGAATACG CAGCTGAATA TGAAGCAGGG
    GTAGAGGAGA TCAGCCTCAC TCCAGATGAG GAATTGCCTC AGTTCTCTAC AGATGACTGT
    GATGTGGAGG CGCCGCCCTC TTACAGCAAG GCTGTCAGCT TTGAGCATCT ATCGTTTGCG
    TCGCAAGATG ACTCTGGGGC CAAGAACCAC ATGGTGGTCA GTCCTGATGA CAGCCGTACA
    GACAAGCTGG AGTCAAGCAT CTTACCACCT CTGACTCATG AGCTGACAGC CAGCGATCTG
    CTGATGAGCA AGATGTTCCA TGATGATGAG CTTGAGGAGT CTGAGAAGTT CTACGTGGAC
    CAGCCCCGGT TTTTGCTGCT ATTCTATGCC ATGTATAACA CCCTGGTGGC CCGCTCAGAA
    ATGGTGTGCT ATTTCGTGAT TATCCTGAAC CACATGACCT CTGCCTCCAT CATCACTCTC
    CTGTTGCCCA TCTTGATCTT CCTCTGGGCC ATGCTGTCTG TTCCCAGGCC CAGCCGACGC
    TTCTGGATGA TGGCCATCGT CTACACTGAG GTGGCGATCG TAGTGAAATA CTTCTTCCAG
    TTCGGCTTCT TTCCCTGGAA TAAGGACTTG GAAATATACA AGGAAAGGCC CTACTTTCCA
    CCGAACATCA TCGGGGTGGA GAAGAAGGAG GGCTATGTTC TTTATGACCT CATTCAGCTG
    CTGGCTCTAT TCTTCCATCG CTCCATTCTG AAGTGTCATG GTCTATGGGA CGAGGATGAC
    ATTGTTGACA GTAACACAGA CAAGGAAGGA TCGGATGACG AGCTGTCCCT CGATCAGGGC
    AGAAGAGGCT CCTCAGACTC ACTGAAGTCT ATCAACCTGG CTGCCTCTGT AGAGTCTGTC
    CACGTGACCT TCCCCGAGCA GCCAGCGGCC ATCAGGAGAA AACGCTCCTG CAGTAGCTCT
    CAGATCTCCC CACGGTCCAG CTTCTCTTCA AATAGGTCCA AAAGAGGCAG CACAAGCACC
    CGGAACAGTA GCCAGAAAGG AAGCAGTGTT TTAAGCCTTA AACAGAAAAG CAAACGAGAA
    CTTTACATGG AAAAGCTTCA AGAGCATTTA ATCAAAGCAA AGGCGTTTAC CATAAAGAAG
    ACTCTACAGA TCTATGTCCC CATTCGGCAA TTCTTCTATG ACCTCATCCA CCCAGACTAC
    AGTGCTGTGA CTGATGTATA CGTGCTGATG TTTCTGGCTG ACACTGTAGA CTTCATTATC
    ATCGTCTTTG GATTCTGGGC CTTTGGGAAA CACTCGGCAG CTGCAGATAT CACCTCTTCT
    CTATCAGAAG ACCAGGTCCC CGGGCCATTC CTGGTGATGG TCCTCATTCA GTTTGGAACC
    ATGGTGGTGG ACCGTGCACT CTACCTCAGG AAGACTGTGC TGGGCAAGGT CATCTTCCAG
    GTCATCCTCG TGTTTGGGAT TCACTTCTGG ATGTTCTTCA TCTTACCCGG TGTGACTGAG
    AGGAAATTCA GCCAGAACCT GGTGGCACAG CTTTGGTACT TTGTGAAGTG TGTGTACTTT
    GGGCTGTCTG CCTATCAAAT CCGCTGTGGC TACCCAACCC GTGTGCTCGG GAACTTCCTC
    ACAAAGAGCT ACAATTACGT CAACCTCTTC TTGTTTCAAG GGTTCCGCCT GGTCCCATTT
    CTGACTGAGC TGCGGGCTGT GATGGATTGG GTGTGGACAG ATACAACCCT GAGCCTCTCC
    AGCTGGATTT GTGTGGAGGA CATTTACGCG CACATTTTTA TCTTAAAGTG CTGGCGAGAG
    TCGGAAAAGA GATACCCTCA ACCCAGGGGT CAGAAGAAGA AGAAAGCCGT GAAGTACGGC
    ATGGGCGGCA TGATCATCGT CCTTCTCATC TGTATCGTCT GGTTTCCTCT GCTCTTCATG
    TCTTTGATCA AATCTGTCGC AGGGGTTATC AACCAACCCC TGGATGTGTC CGTCACAATT
    ACCCTGGGAG GTTATCAGCC TATCTTCACA ATGAGCGCCC AGCAAAGCCA GCTGAAAGTT
    ATGGATAATT CAAAGTATAA TGAATTTCTA AAATCTTTCG GCCCCAACAG TGGCGCAATG
    CAATTTCTGG AGAACTATGA AAGGGAAGAC GTAACAGTAG CAGAACTGGA AGGGAACTCG
    AATTCTTTGT GGACCATCAG CCCTCCCAGT AAGCAGAAAA TGATACAGGA GCTCACCGAC
    CCCAATAGTT GCTTCTCTGT TGTTTTTTCA TGGAGTATCC AGAGAAACAT GACTTTGGGT
    GCAAAAGCCG AAATAGCAAC GGATAAGCTT TCTTTCCCTC TTGCGGTCGC AACACGGAAT
    AGCATAGCTA AAATGATTGC TGGCAATGAC ACAGAAAGTT CAAACACGCC AGTGACAATA
    GAGAAGATCT ACCCATACTA TGTGAAGGCA CCCAGCGATT CAAACTCAAA GCCTATAAAA
    CAGCTTCTGT CTGAAAATAA TTTCATGAAT ATCACCATCA TTTTGTTCAG AGACAATGTC
    ACAAAGTCCA ATAGTGAGTG GTGGGTTCTC AACCTGACCG GAAGTAGGAT ATTCAACCAG
    GGGTCCCAAG CCTTGGAACT GGTGGTCTTC AATGACAAAG TCAGCCCTCC AAGTCTAGGC
    TTCTTGGCTG GCTATGGTAT CATGGGATTG TATGCATCTG TTGTCCTTGT AATTGGGAAA
    TTTGTTCGTG AGTTCTTCAG TGGGATCTCT CATTCCATCA TGTTTGAAGA GCTTCCAAAT
    GTGGACAGAA TCTTGAAGTT GTGCACAGAT ATATTCCTCG TGAGGGAGAC AGGGGAGTTG
    GAACTGGAGG AAGACCTCTA CGCCAAGTTG ATATTCCTGT ACAGGTCACC AGAAACCATG
    ATCAAGTGGA CTAGAGAAAA AACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526232.1
    CDS1448..9937
    Translation

    Target ORF information:

    RefSeq Version XM_006526169.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526169.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    GGCCTGCTGG TGACCGTGAA TGGCAACCCT GTGGACTACC ACACCATCCA CCCAAGCCTG
    CCCATAGAGA ATGGCCCTGC CAAGACGGAC CTGTACACCA CTCCCCAGTA CCGGTGGGAG
    CCTTCGGAGG AGTCCTCAGA AAAGAAAGAG GAAGAAGAGG ACAAGAGAGA AGACTCCGAA
    GGGGAAGGGA GCCAGGAGGA GAAAAGGAGT GTGAGAATGC ACGCCATGGT CGCCGTGTTC
    CAGTTCATCA TGAAGCAGAG TTACATCTGT GCCCTCATCG CCATGATGGC ATGGAGCATC
    ACCTACCACA GCTGGCTGAC TTTCGTGCTG TTGATCTGGT CCTGCACCCT CTGGATGATT
    CGCAACAGGA GGAAGTATGC CATGATCAGT TCTCCTTTCA TGGTCGTCTA TGCGAATCTG
    CTGCTGGTGC TGCAGTACAT ATGGAGCTTC GAGCTCCCCG AGATTAAGAA GGTCCCCGGG
    TTTCTAGAAA AGAAAGAGCC GGGAGAACTT GCTTCAAAGA TTCTCTTCAC CATCACGTTC
    TGGCTGCTCC TGAGGCAGCA TCTCACAGAG CAGAAGGCCC TGAGGGAAAA GGAAGCACTT
    CTTTCTGAAG TCAAAATTGG AAGTCAAGAA CTTGAAGAAA AGGATGAGGA GCTCCAAGAT
    GTACAAGTGG AAGGGGAGCC CACAGAGAAG GAAGAAGAAG AGGAAGAGGA AATAAAGGAA
    GAAAGGCATG AGGTAAAGAA GGAAGAGGAA GAGGAAGTGG AGGAAGATGA TGACCAGGAC
    ATCATGAAGG TGCTGGGTAA TCTGGTGGTG GCCCTCTTCA TCAAGTATTG GATCTACGTC
    TGCGGGGGCA TGTTCTTTTT TGTCAGCTTT GAGGGGAAAA TCGTCATGTA CAAAATCATC
    TACATGGTGC TGTTCCTGTT CTGCGTGGCC TTGTACCAGG TGCACTACGA GTGGTGGAGG
    AAGATCCTGA AGTACTTCTG GATGTCGGTG GTCATTTATA CCATGTTGGT GCTCATCTTC
    ATATACACTT ACCAGTTTGA AAACTTCCCA GGCCTGTGGC AAAACATGAC CGGATTAAAG
    AAAGAAAAGC TCGAGGACCT CGGCCTGAAG CAGTTCACGG TCGCTGAACT GTTCACTCGC
    ATATTCATCC CCACCTCCTT TCTACTCGTC TGCATCCTAC ACCTCCACTA CTTCCACGAT
    CGGTTCCTGG AACTTACAGA CCTCAAGTCC ATCCCCAGCA AAGAAGACAA CACCATCTAC
    AGGTTGGCCC ACCCTGAAGG AAGTCTTCCA GACTTAGCCA TCATGAATAT GACGGCCAGC
    CTTGATAAGC CTGAGGTGCA GAAGTTGGCA GAGTCTGGGG AGGAGAGGCC AGAAGAGTGT
    GTCAAGAAGA CGGAGAAGGG TGAGGCTGGG AAAGACAGTG ATGAGTCTGA GGAGGAGGAA
    GATGAAGAAG AGGAATCCGA GGAAGAAGAA AGCTCAGACC TCAGGAACAA GTGGCACCTG
    GTGATTGACC GGCTCACTGT GCTCTTTCTA AAATTCCTGG AGTATTTCCA CAAGTTGCAG
    GTGTTTATGT GGTGGATCTT GGAGCTGCAC ATCATCAAAA TCGTCTCATC GTACATTATC
    TGGGTGACTG TGAAAGAGGT GTCTCTGTTC AACTATGTAT TTTTGATTTC TTGGGCATTT
    GCTCTGCCAT ATGCCAAACT CCGTCGTGCG GCTTCCAGTG TCTGCACTGT CTGGACATGT
    GTGATCATCG TCTGCAAAAT GTTGTACCAG CTGCAAACCA TTAAACCTGA GAACTTTTCT
    GTCAACTGCT CCTTGCCCAA TGAAAATCAA ACCAACATTC CCCTTCATGA GTTGAACAAG
    TCTCTTCTCT ACAGTGCCCC TGTTGATCCC ACTGAGTGGG TAGGCCTGAG GAAGTCCTCT
    CCTTTGCTCG TCTACCTGAG GAACAACCTC CTGATGCTGG CGATTTTGGC CTTTGAAGTC
    ACCGTTTACC GCCATCAGGA GTACTATCGA GGCCGAAACA ACCTGACTGC TCCAGTGTCT
    AAAACTATCT TTCATGACAT CACAAGGTTA CATCTAGATG ATGGGCTTAT CAATTGTGCC
    AAATATTTTG TAAATTACTT CTTCTACAAG TTTGGCCTAG AGACCTGTTT CCTAATGTCC
    GTGAATGTAA TTGGACAGCG AATGGACTTC TACGCCATGA TTCATGCCTG TTGGTTGATC
    GGTGTCTTGT ATCGACGCAG AAGAAAAGCC ATTGCCGAGG TCTGGCCCAA GTACTGCTGC
    TTCCTGGCTT GCATCATCAC CTTCCAGTAC TTCGTCTGCA TCGGCATCCC CCCTGCCCCT
    TGCCGAGATT ATCCATGGAG ATTTAAGGGA GCCTACTTCA ATGATAACAT TATCAAGTGG
    CTGTACTTCC CGGATTTCAT TGTGCGGCCC AACCCCGTGT TTCTTGTCTA TGACTTCATG
    CTGCTCCTGT GTGCTTCCTT ACAACGACAG ATCTTTGAGG ACGAAAACAA GGCAGCCGTG
    CGCATCATGG CCGGGGACAA TGTCGAGATC TGCATGAACC TTGATGCAGC CTCGTTCAGC
    CAGCATAACC CTGTGCCAGA TTTCATTCAC TGCAGATCTT ACTTGGACAT GTCCAAAGTG
    ATCATCTTCA GCTACCTCTT CTGGTTCGTC CTCACCATCA TCTTCATTAC GGGAACAACC
    AGGATCAGCA TATTTTGCAT GGGCTACTTG GTGGCCTGTT TCTATTTCCT GCTCTTTGGA
    GGGGATCTGC TGCTGAAGCC CATCAAGAGC ATCCTTCGTT ACTGGGACTG GCTGATTGCA
    TACAACGTGT TTGTGATCAC CATGAAGAAT ATACTGTCAA TAGGAGCCTG TGGATACATT
    GGCGCACTAG TAAGAAACAG CTGCTGGTTG ATCCAAGCGT TCAGCCTGGC CTGCACCGTG
    AAAGGCTATC AGATGCCTGA AGATGACTCC AGATGCAAGC TGCCCAGTGG GGAGGCAGGG
    ATCATCTGGG ACAGCATCTG CTTTGCGTTT CTGCTGCTGC AGAGGAGAGT CTTCATGAGC
    TACTACTTCC TGCATGTCGT GGCTGACATT AAAGCATCCC AGATCCTGGC ATCCAGAGGG
    GCCGAACTTT TCCAGGCTAC AATTGTGAAA GCTGTAAAGG CAAGAATTGA AGAAGAGAAA
    AAATCCATGG ACCAGCTGAA GAGACAGATG GATCGCATCA AAGCCCGGCA ACAAAAGTAC
    AAAAAGGGTA AGGAGAGGAT GCTGAGCTTG ACCCAGGAGT CAGGGGAAGG CCAGGACATC
    CAGAAAGTCT CAGAAGAAGA TGATGAAAGA GAAGCAGACA AACAGAAAGC CAAGGGCAAA
    AAAAAGCAGT GGTGGCGGCC TTGGGTTGAT CATGCTTCCA TGGTCAGGAG TGGAGATTAT
    TATTTGTTTG AAACGGATAG TGAAGAGGAG GAAGAAGAGG AACTTAAAAA GGAGGATGAA
    GAACCCCCAC GGAAGTCAGC ATTCCAGAGA GCTATTGGGA AGTTTGCCTC AGCCATTTTA
    GCCTTGCCAA AGTCTGTCAT TAAACTTCCC AAAACTATTC TTCAGTATTT AATCAGAGCT
    GCAAAGTTCG TCTACCAAGC CTGGATTACT GATCCTAAAA CGGCACTCCG ACAGAGGAGG
    AAAGAGAAGA AAAAGTTGGC CAGAGAAGAG CAGAAGGAGA GACGCAAAGG ATCTGGGGAC
    GGTCCAGTGG AATGGGAAGA TCGAGAGGAT GAGCCAGTCA AAAAGAAATC TGATGGACCA
    GATAATATCA TCAAGAGGAT ATTTAATATC CTGAAGTTCA CATGGGTTCT GTTCCTGGCC
    ACAGTGGATA GTTTCACCAC TTGGCTCAAC TCCATCTCAA GGGAACACAT CGACATATCC
    ACAGTCCTGC GGATTGAAAG ATGCATGCTG ACTCGAGAGA TAAAAAAGGG CAATGTTCCA
    ACACGGGAAA GCATCCACAT GTACTACCAG AACCACATTA TGAACCTTTC CCGAGAATCC
    GGCCTGGACA CAATCGACGA GCACTCGGGG GCTGGCTCCA GGGCCCAGGC AGCACATAGG
    ATGGATAGCT TAGATTCCCG GGACAGTATC TCCAGCTGCT ACACTGAAGC GACCCTGCTG
    ATCTCAAGGC AGTCCACCCT TGATGATTTA GATGGACAAG ACCCCGTTCC TAAAACAAGC
    GAGCGTGCTC GGCCTAGGCT CCGTAAAATG TTCAGCCTGG ACATGTCCTC TTCGTCAGCT
    GACAGTGGCA GTGTAGCATC AAGCGAGCCC ACACAGTGTA CCATGCTGTA TTCGCGCCAA
    GGAACTACGG AAACCATCGA GGAGGTGGAG GCTGAAGCTG AGGAGGAGGT GGTAGAAGGT
    CTGGAGCCTG AGCTCCATGA TGCAGAAGAA AAGGAATACG CAGCTGAATA TGAAGCAGGG
    GTAGAGGAGA TCAGCCTCAC TCCAGATGAG GAATTGCCTC AGTTCTCTAC AGATGACTGT
    GATGTGGAGG CGCCGCCCTC TTACAGCAAG GCTGTCAGCT TTGAGCATCT ATCGTTTGCG
    TCGCAAGATG ACTCTGGGGC CAAGAACCAC ATGGTGGTCA GTCCTGATGA CAGCCGTACA
    GACAAGCTGG AGTCAAGCAT CTTACCACCT CTGACTCATG AGCTGACAGC CAGCGATCTG
    CTGATGAGCA AGATGTTCCA TGATGATGAG CTTGAGGAGT CTGAGAAGTT CTACGTGGAC
    CAGCCCCGGT TTTTGCTGCT ATTCTATGCC ATGTATAACA CCCTGGTGGC CCGCTCAGAA
    ATGGTGTGCT ATTTCGTGAT TATCCTGAAC CACATGACCT CTGCCTCCAT CATCACTCTC
    CTGTTGCCCA TCTTGATCTT CCTCTGGGCC ATGCTGTCTG TTCCCAGGCC CAGCCGACGC
    TTCTGGATGA TGGCCATCGT CTACACTGAG GTGGCGATCG TAGTGAAATA CTTCTTCCAG
    TTCGGCTTCT TTCCCTGGAA TAAGGACTTG GAAATATACA AGGAAAGGCC CTACTTTCCA
    CCGAACATCA TCGGGGTGGA GAAGAAGGAG GGCTATGTTC TTTATGACCT CATTCAGCTG
    CTGGCTCTAT TCTTCCATCG CTCCATTCTG AAGTGTCATG GTCTATGGGA CGAGGATGAC
    ATTGTTGACA GTAACACAGA CAAGGAAGGA TCGGATGACG AGCTGTCCCT CGATCAGGGC
    AGAAGAGGCT CCTCAGACTC ACTGAAGTCT ATCAACCTGG CTGCCTCTGT AGAGTCTGTC
    CACGTGACCT TCCCCGAGCA GCCAGCGGCC ATCAGGAGAA AACGCTCCTG CAGTAGCTCT
    CAGATCTCCC CACGGTCCAG CTTCTCTTCA AATAGGTCCA AAAGAGGCAG CACAAGCACC
    CGGAACAGTA GCCAGAAAGG AAGCAGTGTT TTAAGCCTTA AACAGAAAAG CAAACGAGAA
    CTTTACATGG AAAAGCTTCA AGAGCATTTA ATCAAAGCAA AGGCGTTTAC CATAAAGAAG
    ACTCTACAGA TCTATGTCCC CATTCGGCAA TTCTTCTATG ACCTCATCCA CCCAGACTAC
    AGTGCTGTGA CTGATGTATA CGTGCTGATG TTTCTGGCTG ACACTGTAGA CTTCATTATC
    ATCGTCTTTG GATTCTGGGC CTTTGGGAAA CACTCGGCAG CTGCAGATAT CACCTCTTCT
    CTATCAGAAG ACCAGGTCCC CGGGCCATTC CTGGTGATGG TCCTCATTCA GTTTGGAACC
    ATGGTGGTGG ACCGTGCACT CTACCTCAGG AAGACTGTGC TGGGCAAGGT CATCTTCCAG
    GTCATCCTCG TGTTTGGGAT TCACTTCTGG ATGTTCTTCA TCTTACCCGG TGTGACTGAG
    AGGAAATTCA GCCAGAACCT GGTGGCACAG CTTTGGTACT TTGTGAAGTG TGTGTACTTT
    GGGCTGTCTG CCTATCAAAT CCGCTGTGGC TACCCAACCC GTGTGCTCGG GAACTTCCTC
    ACAAAGAGCT ACAATTACGT CAACCTCTTC TTGTTTCAAG GGTTCCGCCT GGTCCCATTT
    CTGACTGAGC TGCGGGCTGT GATGGATTGG GTGTGGACAG ATACAACCCT GAGCCTCTCC
    AGCTGGATTT GTGTGGAGGA CATTTACGCG CACATTTTTA TCTTAAAGTG CTGGCGAGAG
    TCGGAAAAGA GATACCCTCA ACCCAGGGGT CAGAAGAAGA AGAAAGCCGT GAAGTACGGC
    ATGGGCGGCA TGATCATCGT CCTTCTCATC TGTATCGTCT GGTTTCCTCT GCTCTTCATG
    TCTTTGATCA AATCTGTCGC AGGGGTTATC AACCAACCCC TGGATGTGTC CGTCACAATT
    ACCCTGGGAG GTTATCAGCC TATCTTCACA ATGAGCGCCC AGCAAAGCCA GCTGAAAGTT
    ATGGATAATT CAAAGTATAA TGAATTTCTA AAATCTTTCG GCCCCAACAG TGGCGCAATG
    CAATTTCTGG AGAACTATGA AAGGGAAGAC GTAACAGTAG CAGAACTGGA AGGGAACTCG
    AATTCTTTGT GGACCATCAG CCCTCCCAGT AAGCAGAAAA TGATACAGGA GCTCACCGAC
    CCCAATAGTT GCTTCTCTGT TGTTTTTTCA TGGAGTATCC AGAGAAACAT GACTTTGGGT
    GCAAAAGCCG AAATAGCAAC GGATAAGCTT TCTTTCCCTC TTGCGGTCGC AACACGGAAT
    AGCATAGCTA AAATGATTGC TGGCAATGAC ACAGAAAGTT CAAACACGCC AGTGACAATA
    GAGAAGATCT ACCCATACTA TGTGAAGGCA CCCAGCGATT CAAACTCAAA GCCTATAAAA
    CAGCTTCTGT CTGAAAATAA TTTCATGAAT ATCACCATCA TTTTGTTCAG AGACAATGTC
    ACAAAGTCCA ATAGTGAGTG GTGGGTTCTC AACCTGACCG GAAGTAGGAT ATTCAACCAG
    GGGTCCCAAG CCTTGGAACT GGTGGTCTTC AATGACAAAG TCAGCCCTCC AAGTCTAGGC
    TTCTTGGCTG GCTATGGTAT CATGGGATTG TATGCATCTG TTGTCCTTGT AATTGGGAAA
    TTTGTTCGTG AGTTCTTCAG TGGGATCTCT CATTCCATCA TGTTTGAAGA GCTTCCAAAT
    GTGGACAGAA TCTTGAAGTT GTGCACAGAT ATATTCCTCG TGAGGGAGAC AGGGGAGTTG
    GAACTGGAGG AAGACCTCTA CGCCAAGTTG ATATTCCTGT ACAGGTCACC AGAAACCATG
    ATCAAGTGGA CTAGAGAAAA AACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54736
    Clone ID Related Accession (Same CDS sequence) XM_006526170.4 , XM_006526170.3
    Accession Version XM_006526170.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526170.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGT TCGTCTACCA AGCCTGGATT ACTGATCCTA AAACGGCACT CCGACAGAGG
    AGGAAAGAGA AGAAAAAGTT GGCCAGAGAA GAGCAGAAGG AGAGACGCAA AGGATCTGGG
    GACGGTCCAG TGGAATGGGA AGATCGAGAG GATGAGCCAG TCAAAAAGAA ATCTGATGGA
    CCAGATAATA TCATCAAGAG GATATTTAAT ATCCTGAAGT TCACATGGGT TCTGTTCCTG
    GCCACAGTGG ATAGTTTCAC CACTTGGCTC AACTCCATCT CAAGGGAACA CATCGACATA
    TCCACAGTCC TGCGGATTGA AAGATGCATG CTGACTCGAG AGATAAAAAA GGGCAATGTT
    CCAACACGGG AAAGCATCCA CATGTACTAC CAGAACCACA TTATGAACCT TTCCCGAGAA
    TCCGGCCTGG ACACAATCGA CGAGCACTCG GGGGCTGGCT CCAGGGCCCA GGCAGCACAT
    AGGATGGATA GCTTAGATTC CCGGGACAGT ATCTCCAGCT GCTACACTGA AGCGACCCTG
    CTGATCTCAA GGCAGTCCAC CCTTGATGAT TTAGATGGAC AAGACCCCGT TCCTAAAACA
    AGCGAGCGTG CTCGGCCTAG GCTCCGTAAA ATGTTCAGCC TGGACATGTC CTCTTCGTCA
    GCTGACAGTG GCAGTGTAGC ATCAAGCGAG CCCACACAGT GTACCATGCT GTATTCGCGC
    CAAGGAACTA CGGAAACCAT CGAGGAGGTG GAGGCTGAAG CTGAGGAGGA GGTGGTAGAA
    GGTCTGGAGC CTGAGCTCCA TGATGCAGAA GAAAAGGAAT ACGCAGCTGA ATATGAAGCA
    GGGGTAGAGG AGATCAGCCT CACTCCAGAT GAGGAATTGC CTCAGTTCTC TACAGATGAC
    TGTGATGTGG AGGCGCCGCC CTCTTACAGC AAGGCTGTCA GCTTTGAGCA TCTATCGTTT
    GCGTCGCAAG ATGACTCTGG GGCCAAGAAC CACATGGTGG TCAGTCCTGA TGACAGCCGT
    ACAGACAAGC TGGAGTCAAG CATCTTACCA CCTCTGACTC ATGAGCTGAC AGCCAGCGAT
    CTGCTGATGA GCAAGATGTT CCATGATGAT GAGCTTGAGG AGTCTGAGAA GTTCTACGTG
    GACCAGCCCC GGTTTTTGCT GCTATTCTAT GCCATGTATA ACACCCTGGT GGCCCGCTCA
    GAAATGGTGT GCTATTTCGT GATTATCCTG AACCACATGA CCTCTGCCTC CATCATCACT
    CTCCTGTTGC CCATCTTGAT CTTCCTCTGG GCCATGCTGT CTGTTCCCAG GCCCAGCCGA
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TCGTAGTGAA ATACTTCTTC
    CAGTTCGGCT TCTTTCCCTG GAATAAGGAC TTGGAAATAT ACAAGGAAAG GCCCTACTTT
    CCACCGAACA TCATCGGGGT GGAGAAGAAG GAGGGCTATG TTCTTTATGA CCTCATTCAG
    CTGCTGGCTC TATTCTTCCA TCGCTCCATT CTGAAGTGTC ATGGTCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCGGATG ACGAGCTGTC CCTCGATCAG
    GGCAGAAGAG GCTCCTCAGA CTCACTGAAG TCTATCAACC TGGCTGCCTC TGTAGAGTCT
    GTCCACGTGA CCTTCCCCGA GCAGCCAGCG GCCATCAGGA GAAAACGCTC CTGCAGTAGC
    TCTCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGGAACA GTAGCCAGAA AGGAAGCAGT GTTTTAAGCC TTAAACAGAA AAGCAAACGA
    GAACTTTACA TGGAAAAGCT TCAAGAGCAT TTAATCAAAG CAAAGGCGTT TACCATAAAG
    AAGACTCTAC AGATCTATGT CCCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGATGT ATACGTGCTG ATGTTTCTGG CTGACACTGT AGACTTCATT
    ATCATCGTCT TTGGATTCTG GGCCTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCTCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTCCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTGGGCAA GGTCATCTTC
    CAGGTCATCC TCGTGTTTGG GATTCACTTC TGGATGTTCT TCATCTTACC CGGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTTTGGT ACTTTGTGAA GTGTGTGTAC
    TTTGGGCTGT CTGCCTATCA AATCCGCTGT GGCTACCCAA CCCGTGTGCT CGGGAACTTC
    CTCACAAAGA GCTACAATTA CGTCAACCTC TTCTTGTTTC AAGGGTTCCG CCTGGTCCCA
    TTTCTGACTG AGCTGCGGGC TGTGATGGAT TGGGTGTGGA CAGATACAAC CCTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCGCACATTT TTATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGTCAGAAGA AGAAGAAAGC CGTGAAGTAC
    GGCATGGGCG GCATGATCAT CGTCCTTCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAATCTGT CGCAGGGGTT ATCAACCAAC CCCTGGATGT GTCCGTCACA
    ATTACCCTGG GAGGTTATCA GCCTATCTTC ACAATGAGCG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATA ATTCAAAGTA TAATGAATTT CTAAAATCTT TCGGCCCCAA CAGTGGCGCA
    ATGCAATTTC TGGAGAACTA TGAAAGGGAA GACGTAACAG TAGCAGAACT GGAAGGGAAC
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTTTG
    GGTGCAAAAG CCGAAATAGC AACGGATAAG CTTTCTTTCC CTCTTGCGGT CGCAACACGG
    AATAGCATAG CTAAAATGAT TGCTGGCAAT GACACAGAAA GTTCAAACAC GCCAGTGACA
    ATAGAGAAGA TCTACCCATA CTATGTGAAG GCACCCAGCG ATTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCTGAAAA TAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CCGGAAGTAG GATATTCAAC
    CAGGGGTCCC AAGCCTTGGA ACTGGTGGTC TTCAATGACA AAGTCAGCCC TCCAAGTCTA
    GGCTTCTTGG CTGGCTATGG TATCATGGGA TTGTATGCAT CTGTTGTCCT TGTAATTGGG
    AAATTTGTTC GTGAGTTCTT CAGTGGGATC TCTCATTCCA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATCTTGAA GTTGTGCACA GATATATTCC TCGTGAGGGA GACAGGGGAG
    TTGGAACTGG AGGAAGACCT CTACGCCAAG TTGATATTCC TGTACAGGTC ACCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526233.1
    CDS1448..9880
    Translation

    Target ORF information:

    RefSeq Version XM_006526170.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526170.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGT TCGTCTACCA AGCCTGGATT ACTGATCCTA AAACGGCACT CCGACAGAGG
    AGGAAAGAGA AGAAAAAGTT GGCCAGAGAA GAGCAGAAGG AGAGACGCAA AGGATCTGGG
    GACGGTCCAG TGGAATGGGA AGATCGAGAG GATGAGCCAG TCAAAAAGAA ATCTGATGGA
    CCAGATAATA TCATCAAGAG GATATTTAAT ATCCTGAAGT TCACATGGGT TCTGTTCCTG
    GCCACAGTGG ATAGTTTCAC CACTTGGCTC AACTCCATCT CAAGGGAACA CATCGACATA
    TCCACAGTCC TGCGGATTGA AAGATGCATG CTGACTCGAG AGATAAAAAA GGGCAATGTT
    CCAACACGGG AAAGCATCCA CATGTACTAC CAGAACCACA TTATGAACCT TTCCCGAGAA
    TCCGGCCTGG ACACAATCGA CGAGCACTCG GGGGCTGGCT CCAGGGCCCA GGCAGCACAT
    AGGATGGATA GCTTAGATTC CCGGGACAGT ATCTCCAGCT GCTACACTGA AGCGACCCTG
    CTGATCTCAA GGCAGTCCAC CCTTGATGAT TTAGATGGAC AAGACCCCGT TCCTAAAACA
    AGCGAGCGTG CTCGGCCTAG GCTCCGTAAA ATGTTCAGCC TGGACATGTC CTCTTCGTCA
    GCTGACAGTG GCAGTGTAGC ATCAAGCGAG CCCACACAGT GTACCATGCT GTATTCGCGC
    CAAGGAACTA CGGAAACCAT CGAGGAGGTG GAGGCTGAAG CTGAGGAGGA GGTGGTAGAA
    GGTCTGGAGC CTGAGCTCCA TGATGCAGAA GAAAAGGAAT ACGCAGCTGA ATATGAAGCA
    GGGGTAGAGG AGATCAGCCT CACTCCAGAT GAGGAATTGC CTCAGTTCTC TACAGATGAC
    TGTGATGTGG AGGCGCCGCC CTCTTACAGC AAGGCTGTCA GCTTTGAGCA TCTATCGTTT
    GCGTCGCAAG ATGACTCTGG GGCCAAGAAC CACATGGTGG TCAGTCCTGA TGACAGCCGT
    ACAGACAAGC TGGAGTCAAG CATCTTACCA CCTCTGACTC ATGAGCTGAC AGCCAGCGAT
    CTGCTGATGA GCAAGATGTT CCATGATGAT GAGCTTGAGG AGTCTGAGAA GTTCTACGTG
    GACCAGCCCC GGTTTTTGCT GCTATTCTAT GCCATGTATA ACACCCTGGT GGCCCGCTCA
    GAAATGGTGT GCTATTTCGT GATTATCCTG AACCACATGA CCTCTGCCTC CATCATCACT
    CTCCTGTTGC CCATCTTGAT CTTCCTCTGG GCCATGCTGT CTGTTCCCAG GCCCAGCCGA
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TCGTAGTGAA ATACTTCTTC
    CAGTTCGGCT TCTTTCCCTG GAATAAGGAC TTGGAAATAT ACAAGGAAAG GCCCTACTTT
    CCACCGAACA TCATCGGGGT GGAGAAGAAG GAGGGCTATG TTCTTTATGA CCTCATTCAG
    CTGCTGGCTC TATTCTTCCA TCGCTCCATT CTGAAGTGTC ATGGTCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCGGATG ACGAGCTGTC CCTCGATCAG
    GGCAGAAGAG GCTCCTCAGA CTCACTGAAG TCTATCAACC TGGCTGCCTC TGTAGAGTCT
    GTCCACGTGA CCTTCCCCGA GCAGCCAGCG GCCATCAGGA GAAAACGCTC CTGCAGTAGC
    TCTCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGGAACA GTAGCCAGAA AGGAAGCAGT GTTTTAAGCC TTAAACAGAA AAGCAAACGA
    GAACTTTACA TGGAAAAGCT TCAAGAGCAT TTAATCAAAG CAAAGGCGTT TACCATAAAG
    AAGACTCTAC AGATCTATGT CCCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGATGT ATACGTGCTG ATGTTTCTGG CTGACACTGT AGACTTCATT
    ATCATCGTCT TTGGATTCTG GGCCTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCTCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTCCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTGGGCAA GGTCATCTTC
    CAGGTCATCC TCGTGTTTGG GATTCACTTC TGGATGTTCT TCATCTTACC CGGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTTTGGT ACTTTGTGAA GTGTGTGTAC
    TTTGGGCTGT CTGCCTATCA AATCCGCTGT GGCTACCCAA CCCGTGTGCT CGGGAACTTC
    CTCACAAAGA GCTACAATTA CGTCAACCTC TTCTTGTTTC AAGGGTTCCG CCTGGTCCCA
    TTTCTGACTG AGCTGCGGGC TGTGATGGAT TGGGTGTGGA CAGATACAAC CCTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCGCACATTT TTATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGTCAGAAGA AGAAGAAAGC CGTGAAGTAC
    GGCATGGGCG GCATGATCAT CGTCCTTCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAATCTGT CGCAGGGGTT ATCAACCAAC CCCTGGATGT GTCCGTCACA
    ATTACCCTGG GAGGTTATCA GCCTATCTTC ACAATGAGCG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATA ATTCAAAGTA TAATGAATTT CTAAAATCTT TCGGCCCCAA CAGTGGCGCA
    ATGCAATTTC TGGAGAACTA TGAAAGGGAA GACGTAACAG TAGCAGAACT GGAAGGGAAC
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTTTG
    GGTGCAAAAG CCGAAATAGC AACGGATAAG CTTTCTTTCC CTCTTGCGGT CGCAACACGG
    AATAGCATAG CTAAAATGAT TGCTGGCAAT GACACAGAAA GTTCAAACAC GCCAGTGACA
    ATAGAGAAGA TCTACCCATA CTATGTGAAG GCACCCAGCG ATTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCTGAAAA TAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CCGGAAGTAG GATATTCAAC
    CAGGGGTCCC AAGCCTTGGA ACTGGTGGTC TTCAATGACA AAGTCAGCCC TCCAAGTCTA
    GGCTTCTTGG CTGGCTATGG TATCATGGGA TTGTATGCAT CTGTTGTCCT TGTAATTGGG
    AAATTTGTTC GTGAGTTCTT CAGTGGGATC TCTCATTCCA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATCTTGAA GTTGTGCACA GATATATTCC TCGTGAGGGA GACAGGGGAG
    TTGGAACTGG AGGAAGACCT CTACGCCAAG TTGATATTCC TGTACAGGTC ACCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54736
    Clone ID Related Accession (Same CDS sequence) XM_006526170.4 , XM_006526170.3
    Accession Version XM_006526170.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGT TCGTCTACCA AGCCTGGATT ACTGATCCTA AAACGGCACT CCGACAGAGG
    AGGAAAGAGA AGAAAAAGTT GGCCAGAGAA GAGCAGAAGG AGAGACGCAA AGGATCTGGG
    GACGGTCCAG TGGAATGGGA AGATCGAGAG GATGAGCCAG TCAAAAAGAA ATCTGATGGA
    CCAGATAATA TCATCAAGAG GATATTTAAT ATCCTGAAGT TCACATGGGT TCTGTTCCTG
    GCCACAGTGG ATAGTTTCAC CACTTGGCTC AACTCCATCT CAAGGGAACA CATCGACATA
    TCCACAGTCC TGCGGATTGA AAGATGCATG CTGACTCGAG AGATAAAAAA GGGCAATGTT
    CCAACACGGG AAAGCATCCA CATGTACTAC CAGAACCACA TTATGAACCT TTCCCGAGAA
    TCCGGCCTGG ACACAATCGA CGAGCACTCG GGGGCTGGCT CCAGGGCCCA GGCAGCACAT
    AGGATGGATA GCTTAGATTC CCGGGACAGT ATCTCCAGCT GCTACACTGA AGCGACCCTG
    CTGATCTCAA GGCAGTCCAC CCTTGATGAT TTAGATGGAC AAGACCCCGT TCCTAAAACA
    AGCGAGCGTG CTCGGCCTAG GCTCCGTAAA ATGTTCAGCC TGGACATGTC CTCTTCGTCA
    GCTGACAGTG GCAGTGTAGC ATCAAGCGAG CCCACACAGT GTACCATGCT GTATTCGCGC
    CAAGGAACTA CGGAAACCAT CGAGGAGGTG GAGGCTGAAG CTGAGGAGGA GGTGGTAGAA
    GGTCTGGAGC CTGAGCTCCA TGATGCAGAA GAAAAGGAAT ACGCAGCTGA ATATGAAGCA
    GGGGTAGAGG AGATCAGCCT CACTCCAGAT GAGGAATTGC CTCAGTTCTC TACAGATGAC
    TGTGATGTGG AGGCGCCGCC CTCTTACAGC AAGGCTGTCA GCTTTGAGCA TCTATCGTTT
    GCGTCGCAAG ATGACTCTGG GGCCAAGAAC CACATGGTGG TCAGTCCTGA TGACAGCCGT
    ACAGACAAGC TGGAGTCAAG CATCTTACCA CCTCTGACTC ATGAGCTGAC AGCCAGCGAT
    CTGCTGATGA GCAAGATGTT CCATGATGAT GAGCTTGAGG AGTCTGAGAA GTTCTACGTG
    GACCAGCCCC GGTTTTTGCT GCTATTCTAT GCCATGTATA ACACCCTGGT GGCCCGCTCA
    GAAATGGTGT GCTATTTCGT GATTATCCTG AACCACATGA CCTCTGCCTC CATCATCACT
    CTCCTGTTGC CCATCTTGAT CTTCCTCTGG GCCATGCTGT CTGTTCCCAG GCCCAGCCGA
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TCGTAGTGAA ATACTTCTTC
    CAGTTCGGCT TCTTTCCCTG GAATAAGGAC TTGGAAATAT ACAAGGAAAG GCCCTACTTT
    CCACCGAACA TCATCGGGGT GGAGAAGAAG GAGGGCTATG TTCTTTATGA CCTCATTCAG
    CTGCTGGCTC TATTCTTCCA TCGCTCCATT CTGAAGTGTC ATGGTCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCGGATG ACGAGCTGTC CCTCGATCAG
    GGCAGAAGAG GCTCCTCAGA CTCACTGAAG TCTATCAACC TGGCTGCCTC TGTAGAGTCT
    GTCCACGTGA CCTTCCCCGA GCAGCCAGCG GCCATCAGGA GAAAACGCTC CTGCAGTAGC
    TCTCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGGAACA GTAGCCAGAA AGGAAGCAGT GTTTTAAGCC TTAAACAGAA AAGCAAACGA
    GAACTTTACA TGGAAAAGCT TCAAGAGCAT TTAATCAAAG CAAAGGCGTT TACCATAAAG
    AAGACTCTAC AGATCTATGT CCCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGATGT ATACGTGCTG ATGTTTCTGG CTGACACTGT AGACTTCATT
    ATCATCGTCT TTGGATTCTG GGCCTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCTCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTCCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTGGGCAA GGTCATCTTC
    CAGGTCATCC TCGTGTTTGG GATTCACTTC TGGATGTTCT TCATCTTACC CGGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTTTGGT ACTTTGTGAA GTGTGTGTAC
    TTTGGGCTGT CTGCCTATCA AATCCGCTGT GGCTACCCAA CCCGTGTGCT CGGGAACTTC
    CTCACAAAGA GCTACAATTA CGTCAACCTC TTCTTGTTTC AAGGGTTCCG CCTGGTCCCA
    TTTCTGACTG AGCTGCGGGC TGTGATGGAT TGGGTGTGGA CAGATACAAC CCTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCGCACATTT TTATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGTCAGAAGA AGAAGAAAGC CGTGAAGTAC
    GGCATGGGCG GCATGATCAT CGTCCTTCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAATCTGT CGCAGGGGTT ATCAACCAAC CCCTGGATGT GTCCGTCACA
    ATTACCCTGG GAGGTTATCA GCCTATCTTC ACAATGAGCG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATA ATTCAAAGTA TAATGAATTT CTAAAATCTT TCGGCCCCAA CAGTGGCGCA
    ATGCAATTTC TGGAGAACTA TGAAAGGGAA GACGTAACAG TAGCAGAACT GGAAGGGAAC
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTTTG
    GGTGCAAAAG CCGAAATAGC AACGGATAAG CTTTCTTTCC CTCTTGCGGT CGCAACACGG
    AATAGCATAG CTAAAATGAT TGCTGGCAAT GACACAGAAA GTTCAAACAC GCCAGTGACA
    ATAGAGAAGA TCTACCCATA CTATGTGAAG GCACCCAGCG ATTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCTGAAAA TAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CCGGAAGTAG GATATTCAAC
    CAGGGGTCCC AAGCCTTGGA ACTGGTGGTC TTCAATGACA AAGTCAGCCC TCCAAGTCTA
    GGCTTCTTGG CTGGCTATGG TATCATGGGA TTGTATGCAT CTGTTGTCCT TGTAATTGGG
    AAATTTGTTC GTGAGTTCTT CAGTGGGATC TCTCATTCCA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATCTTGAA GTTGTGCACA GATATATTCC TCGTGAGGGA GACAGGGGAG
    TTGGAACTGG AGGAAGACCT CTACGCCAAG TTGATATTCC TGTACAGGTC ACCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526233.1
    CDS1040..9472
    Translation

    Target ORF information:

    RefSeq Version XM_006526170.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526170.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGT TCGTCTACCA AGCCTGGATT ACTGATCCTA AAACGGCACT CCGACAGAGG
    AGGAAAGAGA AGAAAAAGTT GGCCAGAGAA GAGCAGAAGG AGAGACGCAA AGGATCTGGG
    GACGGTCCAG TGGAATGGGA AGATCGAGAG GATGAGCCAG TCAAAAAGAA ATCTGATGGA
    CCAGATAATA TCATCAAGAG GATATTTAAT ATCCTGAAGT TCACATGGGT TCTGTTCCTG
    GCCACAGTGG ATAGTTTCAC CACTTGGCTC AACTCCATCT CAAGGGAACA CATCGACATA
    TCCACAGTCC TGCGGATTGA AAGATGCATG CTGACTCGAG AGATAAAAAA GGGCAATGTT
    CCAACACGGG AAAGCATCCA CATGTACTAC CAGAACCACA TTATGAACCT TTCCCGAGAA
    TCCGGCCTGG ACACAATCGA CGAGCACTCG GGGGCTGGCT CCAGGGCCCA GGCAGCACAT
    AGGATGGATA GCTTAGATTC CCGGGACAGT ATCTCCAGCT GCTACACTGA AGCGACCCTG
    CTGATCTCAA GGCAGTCCAC CCTTGATGAT TTAGATGGAC AAGACCCCGT TCCTAAAACA
    AGCGAGCGTG CTCGGCCTAG GCTCCGTAAA ATGTTCAGCC TGGACATGTC CTCTTCGTCA
    GCTGACAGTG GCAGTGTAGC ATCAAGCGAG CCCACACAGT GTACCATGCT GTATTCGCGC
    CAAGGAACTA CGGAAACCAT CGAGGAGGTG GAGGCTGAAG CTGAGGAGGA GGTGGTAGAA
    GGTCTGGAGC CTGAGCTCCA TGATGCAGAA GAAAAGGAAT ACGCAGCTGA ATATGAAGCA
    GGGGTAGAGG AGATCAGCCT CACTCCAGAT GAGGAATTGC CTCAGTTCTC TACAGATGAC
    TGTGATGTGG AGGCGCCGCC CTCTTACAGC AAGGCTGTCA GCTTTGAGCA TCTATCGTTT
    GCGTCGCAAG ATGACTCTGG GGCCAAGAAC CACATGGTGG TCAGTCCTGA TGACAGCCGT
    ACAGACAAGC TGGAGTCAAG CATCTTACCA CCTCTGACTC ATGAGCTGAC AGCCAGCGAT
    CTGCTGATGA GCAAGATGTT CCATGATGAT GAGCTTGAGG AGTCTGAGAA GTTCTACGTG
    GACCAGCCCC GGTTTTTGCT GCTATTCTAT GCCATGTATA ACACCCTGGT GGCCCGCTCA
    GAAATGGTGT GCTATTTCGT GATTATCCTG AACCACATGA CCTCTGCCTC CATCATCACT
    CTCCTGTTGC CCATCTTGAT CTTCCTCTGG GCCATGCTGT CTGTTCCCAG GCCCAGCCGA
    CGCTTCTGGA TGATGGCCAT CGTCTACACT GAGGTGGCGA TCGTAGTGAA ATACTTCTTC
    CAGTTCGGCT TCTTTCCCTG GAATAAGGAC TTGGAAATAT ACAAGGAAAG GCCCTACTTT
    CCACCGAACA TCATCGGGGT GGAGAAGAAG GAGGGCTATG TTCTTTATGA CCTCATTCAG
    CTGCTGGCTC TATTCTTCCA TCGCTCCATT CTGAAGTGTC ATGGTCTATG GGACGAGGAT
    GACATTGTTG ACAGTAACAC AGACAAGGAA GGATCGGATG ACGAGCTGTC CCTCGATCAG
    GGCAGAAGAG GCTCCTCAGA CTCACTGAAG TCTATCAACC TGGCTGCCTC TGTAGAGTCT
    GTCCACGTGA CCTTCCCCGA GCAGCCAGCG GCCATCAGGA GAAAACGCTC CTGCAGTAGC
    TCTCAGATCT CCCCACGGTC CAGCTTCTCT TCAAATAGGT CCAAAAGAGG CAGCACAAGC
    ACCCGGAACA GTAGCCAGAA AGGAAGCAGT GTTTTAAGCC TTAAACAGAA AAGCAAACGA
    GAACTTTACA TGGAAAAGCT TCAAGAGCAT TTAATCAAAG CAAAGGCGTT TACCATAAAG
    AAGACTCTAC AGATCTATGT CCCCATTCGG CAATTCTTCT ATGACCTCAT CCACCCAGAC
    TACAGTGCTG TGACTGATGT ATACGTGCTG ATGTTTCTGG CTGACACTGT AGACTTCATT
    ATCATCGTCT TTGGATTCTG GGCCTTTGGG AAACACTCGG CAGCTGCAGA TATCACCTCT
    TCTCTATCAG AAGACCAGGT CCCCGGGCCA TTCCTGGTGA TGGTCCTCAT TCAGTTTGGA
    ACCATGGTGG TGGACCGTGC ACTCTACCTC AGGAAGACTG TGCTGGGCAA GGTCATCTTC
    CAGGTCATCC TCGTGTTTGG GATTCACTTC TGGATGTTCT TCATCTTACC CGGTGTGACT
    GAGAGGAAAT TCAGCCAGAA CCTGGTGGCA CAGCTTTGGT ACTTTGTGAA GTGTGTGTAC
    TTTGGGCTGT CTGCCTATCA AATCCGCTGT GGCTACCCAA CCCGTGTGCT CGGGAACTTC
    CTCACAAAGA GCTACAATTA CGTCAACCTC TTCTTGTTTC AAGGGTTCCG CCTGGTCCCA
    TTTCTGACTG AGCTGCGGGC TGTGATGGAT TGGGTGTGGA CAGATACAAC CCTGAGCCTC
    TCCAGCTGGA TTTGTGTGGA GGACATTTAC GCGCACATTT TTATCTTAAA GTGCTGGCGA
    GAGTCGGAAA AGAGATACCC TCAACCCAGG GGTCAGAAGA AGAAGAAAGC CGTGAAGTAC
    GGCATGGGCG GCATGATCAT CGTCCTTCTC ATCTGTATCG TCTGGTTTCC TCTGCTCTTC
    ATGTCTTTGA TCAAATCTGT CGCAGGGGTT ATCAACCAAC CCCTGGATGT GTCCGTCACA
    ATTACCCTGG GAGGTTATCA GCCTATCTTC ACAATGAGCG CCCAGCAAAG CCAGCTGAAA
    GTTATGGATA ATTCAAAGTA TAATGAATTT CTAAAATCTT TCGGCCCCAA CAGTGGCGCA
    ATGCAATTTC TGGAGAACTA TGAAAGGGAA GACGTAACAG TAGCAGAACT GGAAGGGAAC
    TCGAATTCTT TGTGGACCAT CAGCCCTCCC AGTAAGCAGA AAATGATACA GGAGCTCACC
    GACCCCAATA GTTGCTTCTC TGTTGTTTTT TCATGGAGTA TCCAGAGAAA CATGACTTTG
    GGTGCAAAAG CCGAAATAGC AACGGATAAG CTTTCTTTCC CTCTTGCGGT CGCAACACGG
    AATAGCATAG CTAAAATGAT TGCTGGCAAT GACACAGAAA GTTCAAACAC GCCAGTGACA
    ATAGAGAAGA TCTACCCATA CTATGTGAAG GCACCCAGCG ATTCAAACTC AAAGCCTATA
    AAACAGCTTC TGTCTGAAAA TAATTTCATG AATATCACCA TCATTTTGTT CAGAGACAAT
    GTCACAAAGT CCAATAGTGA GTGGTGGGTT CTCAACCTGA CCGGAAGTAG GATATTCAAC
    CAGGGGTCCC AAGCCTTGGA ACTGGTGGTC TTCAATGACA AAGTCAGCCC TCCAAGTCTA
    GGCTTCTTGG CTGGCTATGG TATCATGGGA TTGTATGCAT CTGTTGTCCT TGTAATTGGG
    AAATTTGTTC GTGAGTTCTT CAGTGGGATC TCTCATTCCA TCATGTTTGA AGAGCTTCCA
    AATGTGGACA GAATCTTGAA GTTGTGCACA GATATATTCC TCGTGAGGGA GACAGGGGAG
    TTGGAACTGG AGGAAGACCT CTACGCCAAG TTGATATTCC TGTACAGGTC ACCAGAAACC
    ATGATCAAGT GGACTAGAGA AAAAACAAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54738
    Clone ID Related Accession (Same CDS sequence) XM_006526172.4 , XM_006526172.3
    Accession Version XM_006526172.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526172.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGA GAGCTATTGG GAAGTTTGCC TCAGCCATTT TAGCCTTGCC AAAGTCTGTC
    ATTAAACTTC CCAAAACTAT TCTTCAGTAT TTAATCAGAG CTGCAAAGTT CGTCTACCAA
    GCCTGGATTA CTGATCCTAA AACGGCACTC CGACAGAGGA GGAAAGAGAA GAAAAAGTTG
    GCCAGAGAAG AGCAGAAGGA GAGACGCAAA GGATCTGGGG ACGGTCCAGT GGAATGGGAA
    GATCGAGAGG ATGAGCCAGT CAAAAAGAAA TCTGATGGAC CAGATAATAT CATCAAGAGG
    ATATTTAATA TCCTGAAGTT CACATGGGTT CTGTTCCTGG CCACAGTGGA TAGTTTCACC
    ACTTGGCTCA ACTCCATCTC AAGGGAACAC ATCGACATAT CCACAGTCCT GCGGATTGAA
    AGATGCATGC TGACTCGAGA GATAAAAAAG GGCAATGTTC CAACACGGGA AAGCATCCAC
    ATGTACTACC AGAACCACAT TATGAACCTT TCCCGAGAAT CCGGCCTGGA CACAATCGAC
    GAGCACTCGG GGGCTGGCTC CAGGGCCCAG GCAGCACATA GGATGGATAG CTTAGATTCC
    CGGGACAGTA TCTCCAGCGA GCCCACACAG TGTACCATGC TGTATTCGCG CCAAGGAACT
    ACGGAAACCA TCGAGGAGGT GGAGGCTGAA GCTGAGGAGG AGGTGGTAGA AGGTCTGGAG
    CCTGAGCTCC ATGATGCAGA AGAAAAGGAA TACGCAGCTG AATATGAAGC AGGGGTAGAG
    GAGATCAGCC TCACTCCAGA TGAGGAATTG CCTCAGTTCT CTACAGATGA CTGTGATGTG
    GAGGCGCCGC CCTCTTACAG CAAGGCTGTC AGCTTTGAGC ATCTATCGTT TGCGTCGCAA
    GATGACTCTG GGGCCAAGAA CCACATGGTG GTCAGTCCTG ATGACAGCCG TACAGACAAG
    CTGGAGTCAA GCATCTTACC ACCTCTGACT CATGAGCTGA CAGCCAGCGA TCTGCTGATG
    AGCAAGATGT TCCATGATGA TGAGCTTGAG GAGTCTGAGA AGTTCTACGT GGACCAGCCC
    CGGTTTTTGC TGCTATTCTA TGCCATGTAT AACACCCTGG TGGCCCGCTC AGAAATGGTG
    TGCTATTTCG TGATTATCCT GAACCACATG ACCTCTGCCT CCATCATCAC TCTCCTGTTG
    CCCATCTTGA TCTTCCTCTG GGCCATGCTG TCTGTTCCCA GGCCCAGCCG ACGCTTCTGG
    ATGATGGCCA TCGTCTACAC TGAGGTGGCG ATCGTAGTGA AATACTTCTT CCAGTTCGGC
    TTCTTTCCCT GGAATAAGGA CTTGGAAATA TACAAGGAAA GGCCCTACTT TCCACCGAAC
    ATCATCGGGG TGGAGAAGAA GGAGGGCTAT GTTCTTTATG ACCTCATTCA GCTGCTGGCT
    CTATTCTTCC ATCGCTCCAT TCTGAAGTGT CATGGTCTAT GGGACGAGGA TGACATTGTT
    GACAGTAACA CAGACAAGGA AGGATCGGAT GACGAGCTGT CCCTCGATCA GGGCAGAAGA
    GGCTCCTCAG ACTCACTGAA GTCTATCAAC CTGGCTGCCT CTGTAGAGTC TGTCCACGTG
    ACCTTCCCCG AGCAGCCAGC GGCCATCAGG AGAAAACGCT CCTGCAGTAG CTCTCAGATC
    TCCCCACGGT CCAGCTTCTC TTCAAATAGG TCCAAAAGAG GCAGCACAAG CACCCGGAAC
    AGTAGCCAGA AAGGAAGCAG TGTTTTAAGC CTTAAACAGA AAAGCAAACG AGAACTTTAC
    ATGGAAAAGC TTCAAGAGCA TTTAATCAAA GCAAAGGCGT TTACCATAAA GAAGACTCTA
    CAGATCTATG TCCCCATTCG GCAATTCTTC TATGACCTCA TCCACCCAGA CTACAGTGCT
    GTGACTGATG TATACGTGCT GATGTTTCTG GCTGACACTG TAGACTTCAT TATCATCGTC
    TTTGGATTCT GGGCCTTTGG GAAACACTCG GCAGCTGCAG ATATCACCTC TTCTCTATCA
    GAAGACCAGG TCCCCGGGCC ATTCCTGGTG ATGGTCCTCA TTCAGTTTGG AACCATGGTG
    GTGGACCGTG CACTCTACCT CAGGAAGACT GTGCTGGGCA AGGTCATCTT CCAGGTCATC
    CTCGTGTTTG GGATTCACTT CTGGATGTTC TTCATCTTAC CCGGTGTGAC TGAGAGGAAA
    TTCAGCCAGA ACCTGGTGGC ACAGCTTTGG TACTTTGTGA AGTGTGTGTA CTTTGGGCTG
    TCTGCCTATC AAATCCGCTG TGGCTACCCA ACCCGTGTGC TCGGGAACTT CCTCACAAAG
    AGCTACAATT ACGTCAACCT CTTCTTGTTT CAAGGGTTCC GCCTGGTCCC ATTTCTGACT
    GAGCTGCGGG CTGTGATGGA TTGGGTGTGG ACAGATACAA CCCTGAGCCT CTCCAGCTGG
    ATTTGTGTGG AGGACATTTA CGCGCACATT TTTATCTTAA AGTGCTGGCG AGAGTCGGAA
    AAGAGATACC CTCAACCCAG GGGTCAGAAG AAGAAGAAAG CCGTGAAGTA CGGCATGGGC
    GGCATGATCA TCGTCCTTCT CATCTGTATC GTCTGGTTTC CTCTGCTCTT CATGTCTTTG
    ATCAAATCTG TCGCAGGGGT TATCAACCAA CCCCTGGATG TGTCCGTCAC AATTACCCTG
    GGAGGTTATC AGCCTATCTT CACAATGAGC GCCCAGCAAA GCCAGCTGAA AGTTATGGAT
    AATTCAAAGT ATAATGAATT TCTAAAATCT TTCGGCCCCA ACAGTGGCGC AATGCAATTT
    CTGGAGAACT ATGAAAGGGA AGACGTAACA GTAGCAGAAC TGGAAGGGAA CTCGAATTCT
    TTGTGGACCA TCAGCCCTCC CAGTAAGCAG AAAATGATAC AGGAGCTCAC CGACCCCAAT
    AGTTGCTTCT CTGTTGTTTT TTCATGGAGT ATCCAGAGAA ACATGACTTT GGGTGCAAAA
    GCCGAAATAG CAACGGATAA GCTTTCTTTC CCTCTTGCGG TCGCAACACG GAATAGCATA
    GCTAAAATGA TTGCTGGCAA TGACACAGAA AGTTCAAACA CGCCAGTGAC AATAGAGAAG
    ATCTACCCAT ACTATGTGAA GGCACCCAGC GATTCAAACT CAAAGCCTAT AAAACAGCTT
    CTGTCTGAAA ATAATTTCAT GAATATCACC ATCATTTTGT TCAGAGACAA TGTCACAAAG
    TCCAATAGTG AGTGGTGGGT TCTCAACCTG ACCGGAAGTA GGATATTCAA CCAGGGGTCC
    CAAGCCTTGG AACTGGTGGT CTTCAATGAC AAAGTCAGCC CTCCAAGTCT AGGCTTCTTG
    GCTGGCTATG GTATCATGGG ATTGTATGCA TCTGTTGTCC TTGTAATTGG GAAATTTGTT
    CGTGAGTTCT TCAGTGGGAT CTCTCATTCC ATCATGTTTG AAGAGCTTCC AAATGTGGAC
    AGAATCTTGA AGTTGTGCAC AGATATATTC CTCGTGAGGG AGACAGGGGA GTTGGAACTG
    GAGGAAGACC TCTACGCCAA GTTGATATTC CTGTACAGGT CACCAGAAAC CATGATCAAG
    TGGACTAGAG AAAAAACAAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526235.1
    CDS1448..9811
    Translation

    Target ORF information:

    RefSeq Version XM_006526172.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526172.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGA GAGCTATTGG GAAGTTTGCC TCAGCCATTT TAGCCTTGCC AAAGTCTGTC
    ATTAAACTTC CCAAAACTAT TCTTCAGTAT TTAATCAGAG CTGCAAAGTT CGTCTACCAA
    GCCTGGATTA CTGATCCTAA AACGGCACTC CGACAGAGGA GGAAAGAGAA GAAAAAGTTG
    GCCAGAGAAG AGCAGAAGGA GAGACGCAAA GGATCTGGGG ACGGTCCAGT GGAATGGGAA
    GATCGAGAGG ATGAGCCAGT CAAAAAGAAA TCTGATGGAC CAGATAATAT CATCAAGAGG
    ATATTTAATA TCCTGAAGTT CACATGGGTT CTGTTCCTGG CCACAGTGGA TAGTTTCACC
    ACTTGGCTCA ACTCCATCTC AAGGGAACAC ATCGACATAT CCACAGTCCT GCGGATTGAA
    AGATGCATGC TGACTCGAGA GATAAAAAAG GGCAATGTTC CAACACGGGA AAGCATCCAC
    ATGTACTACC AGAACCACAT TATGAACCTT TCCCGAGAAT CCGGCCTGGA CACAATCGAC
    GAGCACTCGG GGGCTGGCTC CAGGGCCCAG GCAGCACATA GGATGGATAG CTTAGATTCC
    CGGGACAGTA TCTCCAGCGA GCCCACACAG TGTACCATGC TGTATTCGCG CCAAGGAACT
    ACGGAAACCA TCGAGGAGGT GGAGGCTGAA GCTGAGGAGG AGGTGGTAGA AGGTCTGGAG
    CCTGAGCTCC ATGATGCAGA AGAAAAGGAA TACGCAGCTG AATATGAAGC AGGGGTAGAG
    GAGATCAGCC TCACTCCAGA TGAGGAATTG CCTCAGTTCT CTACAGATGA CTGTGATGTG
    GAGGCGCCGC CCTCTTACAG CAAGGCTGTC AGCTTTGAGC ATCTATCGTT TGCGTCGCAA
    GATGACTCTG GGGCCAAGAA CCACATGGTG GTCAGTCCTG ATGACAGCCG TACAGACAAG
    CTGGAGTCAA GCATCTTACC ACCTCTGACT CATGAGCTGA CAGCCAGCGA TCTGCTGATG
    AGCAAGATGT TCCATGATGA TGAGCTTGAG GAGTCTGAGA AGTTCTACGT GGACCAGCCC
    CGGTTTTTGC TGCTATTCTA TGCCATGTAT AACACCCTGG TGGCCCGCTC AGAAATGGTG
    TGCTATTTCG TGATTATCCT GAACCACATG ACCTCTGCCT CCATCATCAC TCTCCTGTTG
    CCCATCTTGA TCTTCCTCTG GGCCATGCTG TCTGTTCCCA GGCCCAGCCG ACGCTTCTGG
    ATGATGGCCA TCGTCTACAC TGAGGTGGCG ATCGTAGTGA AATACTTCTT CCAGTTCGGC
    TTCTTTCCCT GGAATAAGGA CTTGGAAATA TACAAGGAAA GGCCCTACTT TCCACCGAAC
    ATCATCGGGG TGGAGAAGAA GGAGGGCTAT GTTCTTTATG ACCTCATTCA GCTGCTGGCT
    CTATTCTTCC ATCGCTCCAT TCTGAAGTGT CATGGTCTAT GGGACGAGGA TGACATTGTT
    GACAGTAACA CAGACAAGGA AGGATCGGAT GACGAGCTGT CCCTCGATCA GGGCAGAAGA
    GGCTCCTCAG ACTCACTGAA GTCTATCAAC CTGGCTGCCT CTGTAGAGTC TGTCCACGTG
    ACCTTCCCCG AGCAGCCAGC GGCCATCAGG AGAAAACGCT CCTGCAGTAG CTCTCAGATC
    TCCCCACGGT CCAGCTTCTC TTCAAATAGG TCCAAAAGAG GCAGCACAAG CACCCGGAAC
    AGTAGCCAGA AAGGAAGCAG TGTTTTAAGC CTTAAACAGA AAAGCAAACG AGAACTTTAC
    ATGGAAAAGC TTCAAGAGCA TTTAATCAAA GCAAAGGCGT TTACCATAAA GAAGACTCTA
    CAGATCTATG TCCCCATTCG GCAATTCTTC TATGACCTCA TCCACCCAGA CTACAGTGCT
    GTGACTGATG TATACGTGCT GATGTTTCTG GCTGACACTG TAGACTTCAT TATCATCGTC
    TTTGGATTCT GGGCCTTTGG GAAACACTCG GCAGCTGCAG ATATCACCTC TTCTCTATCA
    GAAGACCAGG TCCCCGGGCC ATTCCTGGTG ATGGTCCTCA TTCAGTTTGG AACCATGGTG
    GTGGACCGTG CACTCTACCT CAGGAAGACT GTGCTGGGCA AGGTCATCTT CCAGGTCATC
    CTCGTGTTTG GGATTCACTT CTGGATGTTC TTCATCTTAC CCGGTGTGAC TGAGAGGAAA
    TTCAGCCAGA ACCTGGTGGC ACAGCTTTGG TACTTTGTGA AGTGTGTGTA CTTTGGGCTG
    TCTGCCTATC AAATCCGCTG TGGCTACCCA ACCCGTGTGC TCGGGAACTT CCTCACAAAG
    AGCTACAATT ACGTCAACCT CTTCTTGTTT CAAGGGTTCC GCCTGGTCCC ATTTCTGACT
    GAGCTGCGGG CTGTGATGGA TTGGGTGTGG ACAGATACAA CCCTGAGCCT CTCCAGCTGG
    ATTTGTGTGG AGGACATTTA CGCGCACATT TTTATCTTAA AGTGCTGGCG AGAGTCGGAA
    AAGAGATACC CTCAACCCAG GGGTCAGAAG AAGAAGAAAG CCGTGAAGTA CGGCATGGGC
    GGCATGATCA TCGTCCTTCT CATCTGTATC GTCTGGTTTC CTCTGCTCTT CATGTCTTTG
    ATCAAATCTG TCGCAGGGGT TATCAACCAA CCCCTGGATG TGTCCGTCAC AATTACCCTG
    GGAGGTTATC AGCCTATCTT CACAATGAGC GCCCAGCAAA GCCAGCTGAA AGTTATGGAT
    AATTCAAAGT ATAATGAATT TCTAAAATCT TTCGGCCCCA ACAGTGGCGC AATGCAATTT
    CTGGAGAACT ATGAAAGGGA AGACGTAACA GTAGCAGAAC TGGAAGGGAA CTCGAATTCT
    TTGTGGACCA TCAGCCCTCC CAGTAAGCAG AAAATGATAC AGGAGCTCAC CGACCCCAAT
    AGTTGCTTCT CTGTTGTTTT TTCATGGAGT ATCCAGAGAA ACATGACTTT GGGTGCAAAA
    GCCGAAATAG CAACGGATAA GCTTTCTTTC CCTCTTGCGG TCGCAACACG GAATAGCATA
    GCTAAAATGA TTGCTGGCAA TGACACAGAA AGTTCAAACA CGCCAGTGAC AATAGAGAAG
    ATCTACCCAT ACTATGTGAA GGCACCCAGC GATTCAAACT CAAAGCCTAT AAAACAGCTT
    CTGTCTGAAA ATAATTTCAT GAATATCACC ATCATTTTGT TCAGAGACAA TGTCACAAAG
    TCCAATAGTG AGTGGTGGGT TCTCAACCTG ACCGGAAGTA GGATATTCAA CCAGGGGTCC
    CAAGCCTTGG AACTGGTGGT CTTCAATGAC AAAGTCAGCC CTCCAAGTCT AGGCTTCTTG
    GCTGGCTATG GTATCATGGG ATTGTATGCA TCTGTTGTCC TTGTAATTGG GAAATTTGTT
    CGTGAGTTCT TCAGTGGGAT CTCTCATTCC ATCATGTTTG AAGAGCTTCC AAATGTGGAC
    AGAATCTTGA AGTTGTGCAC AGATATATTC CTCGTGAGGG AGACAGGGGA GTTGGAACTG
    GAGGAAGACC TCTACGCCAA GTTGATATTC CTGTACAGGT CACCAGAAAC CATGATCAAG
    TGGACTAGAG AAAAAACAAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54738
    Clone ID Related Accession (Same CDS sequence) XM_006526172.4 , XM_006526172.3
    Accession Version XM_006526172.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    4141
    4201
    4261
    4321
    4381
    4441
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    4681
    4741
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    4921
    4981
    5041
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    5161
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    5281
    5341
    5401
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    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6781
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    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGA GAGCTATTGG GAAGTTTGCC TCAGCCATTT TAGCCTTGCC AAAGTCTGTC
    ATTAAACTTC CCAAAACTAT TCTTCAGTAT TTAATCAGAG CTGCAAAGTT CGTCTACCAA
    GCCTGGATTA CTGATCCTAA AACGGCACTC CGACAGAGGA GGAAAGAGAA GAAAAAGTTG
    GCCAGAGAAG AGCAGAAGGA GAGACGCAAA GGATCTGGGG ACGGTCCAGT GGAATGGGAA
    GATCGAGAGG ATGAGCCAGT CAAAAAGAAA TCTGATGGAC CAGATAATAT CATCAAGAGG
    ATATTTAATA TCCTGAAGTT CACATGGGTT CTGTTCCTGG CCACAGTGGA TAGTTTCACC
    ACTTGGCTCA ACTCCATCTC AAGGGAACAC ATCGACATAT CCACAGTCCT GCGGATTGAA
    AGATGCATGC TGACTCGAGA GATAAAAAAG GGCAATGTTC CAACACGGGA AAGCATCCAC
    ATGTACTACC AGAACCACAT TATGAACCTT TCCCGAGAAT CCGGCCTGGA CACAATCGAC
    GAGCACTCGG GGGCTGGCTC CAGGGCCCAG GCAGCACATA GGATGGATAG CTTAGATTCC
    CGGGACAGTA TCTCCAGCGA GCCCACACAG TGTACCATGC TGTATTCGCG CCAAGGAACT
    ACGGAAACCA TCGAGGAGGT GGAGGCTGAA GCTGAGGAGG AGGTGGTAGA AGGTCTGGAG
    CCTGAGCTCC ATGATGCAGA AGAAAAGGAA TACGCAGCTG AATATGAAGC AGGGGTAGAG
    GAGATCAGCC TCACTCCAGA TGAGGAATTG CCTCAGTTCT CTACAGATGA CTGTGATGTG
    GAGGCGCCGC CCTCTTACAG CAAGGCTGTC AGCTTTGAGC ATCTATCGTT TGCGTCGCAA
    GATGACTCTG GGGCCAAGAA CCACATGGTG GTCAGTCCTG ATGACAGCCG TACAGACAAG
    CTGGAGTCAA GCATCTTACC ACCTCTGACT CATGAGCTGA CAGCCAGCGA TCTGCTGATG
    AGCAAGATGT TCCATGATGA TGAGCTTGAG GAGTCTGAGA AGTTCTACGT GGACCAGCCC
    CGGTTTTTGC TGCTATTCTA TGCCATGTAT AACACCCTGG TGGCCCGCTC AGAAATGGTG
    TGCTATTTCG TGATTATCCT GAACCACATG ACCTCTGCCT CCATCATCAC TCTCCTGTTG
    CCCATCTTGA TCTTCCTCTG GGCCATGCTG TCTGTTCCCA GGCCCAGCCG ACGCTTCTGG
    ATGATGGCCA TCGTCTACAC TGAGGTGGCG ATCGTAGTGA AATACTTCTT CCAGTTCGGC
    TTCTTTCCCT GGAATAAGGA CTTGGAAATA TACAAGGAAA GGCCCTACTT TCCACCGAAC
    ATCATCGGGG TGGAGAAGAA GGAGGGCTAT GTTCTTTATG ACCTCATTCA GCTGCTGGCT
    CTATTCTTCC ATCGCTCCAT TCTGAAGTGT CATGGTCTAT GGGACGAGGA TGACATTGTT
    GACAGTAACA CAGACAAGGA AGGATCGGAT GACGAGCTGT CCCTCGATCA GGGCAGAAGA
    GGCTCCTCAG ACTCACTGAA GTCTATCAAC CTGGCTGCCT CTGTAGAGTC TGTCCACGTG
    ACCTTCCCCG AGCAGCCAGC GGCCATCAGG AGAAAACGCT CCTGCAGTAG CTCTCAGATC
    TCCCCACGGT CCAGCTTCTC TTCAAATAGG TCCAAAAGAG GCAGCACAAG CACCCGGAAC
    AGTAGCCAGA AAGGAAGCAG TGTTTTAAGC CTTAAACAGA AAAGCAAACG AGAACTTTAC
    ATGGAAAAGC TTCAAGAGCA TTTAATCAAA GCAAAGGCGT TTACCATAAA GAAGACTCTA
    CAGATCTATG TCCCCATTCG GCAATTCTTC TATGACCTCA TCCACCCAGA CTACAGTGCT
    GTGACTGATG TATACGTGCT GATGTTTCTG GCTGACACTG TAGACTTCAT TATCATCGTC
    TTTGGATTCT GGGCCTTTGG GAAACACTCG GCAGCTGCAG ATATCACCTC TTCTCTATCA
    GAAGACCAGG TCCCCGGGCC ATTCCTGGTG ATGGTCCTCA TTCAGTTTGG AACCATGGTG
    GTGGACCGTG CACTCTACCT CAGGAAGACT GTGCTGGGCA AGGTCATCTT CCAGGTCATC
    CTCGTGTTTG GGATTCACTT CTGGATGTTC TTCATCTTAC CCGGTGTGAC TGAGAGGAAA
    TTCAGCCAGA ACCTGGTGGC ACAGCTTTGG TACTTTGTGA AGTGTGTGTA CTTTGGGCTG
    TCTGCCTATC AAATCCGCTG TGGCTACCCA ACCCGTGTGC TCGGGAACTT CCTCACAAAG
    AGCTACAATT ACGTCAACCT CTTCTTGTTT CAAGGGTTCC GCCTGGTCCC ATTTCTGACT
    GAGCTGCGGG CTGTGATGGA TTGGGTGTGG ACAGATACAA CCCTGAGCCT CTCCAGCTGG
    ATTTGTGTGG AGGACATTTA CGCGCACATT TTTATCTTAA AGTGCTGGCG AGAGTCGGAA
    AAGAGATACC CTCAACCCAG GGGTCAGAAG AAGAAGAAAG CCGTGAAGTA CGGCATGGGC
    GGCATGATCA TCGTCCTTCT CATCTGTATC GTCTGGTTTC CTCTGCTCTT CATGTCTTTG
    ATCAAATCTG TCGCAGGGGT TATCAACCAA CCCCTGGATG TGTCCGTCAC AATTACCCTG
    GGAGGTTATC AGCCTATCTT CACAATGAGC GCCCAGCAAA GCCAGCTGAA AGTTATGGAT
    AATTCAAAGT ATAATGAATT TCTAAAATCT TTCGGCCCCA ACAGTGGCGC AATGCAATTT
    CTGGAGAACT ATGAAAGGGA AGACGTAACA GTAGCAGAAC TGGAAGGGAA CTCGAATTCT
    TTGTGGACCA TCAGCCCTCC CAGTAAGCAG AAAATGATAC AGGAGCTCAC CGACCCCAAT
    AGTTGCTTCT CTGTTGTTTT TTCATGGAGT ATCCAGAGAA ACATGACTTT GGGTGCAAAA
    GCCGAAATAG CAACGGATAA GCTTTCTTTC CCTCTTGCGG TCGCAACACG GAATAGCATA
    GCTAAAATGA TTGCTGGCAA TGACACAGAA AGTTCAAACA CGCCAGTGAC AATAGAGAAG
    ATCTACCCAT ACTATGTGAA GGCACCCAGC GATTCAAACT CAAAGCCTAT AAAACAGCTT
    CTGTCTGAAA ATAATTTCAT GAATATCACC ATCATTTTGT TCAGAGACAA TGTCACAAAG
    TCCAATAGTG AGTGGTGGGT TCTCAACCTG ACCGGAAGTA GGATATTCAA CCAGGGGTCC
    CAAGCCTTGG AACTGGTGGT CTTCAATGAC AAAGTCAGCC CTCCAAGTCT AGGCTTCTTG
    GCTGGCTATG GTATCATGGG ATTGTATGCA TCTGTTGTCC TTGTAATTGG GAAATTTGTT
    CGTGAGTTCT TCAGTGGGAT CTCTCATTCC ATCATGTTTG AAGAGCTTCC AAATGTGGAC
    AGAATCTTGA AGTTGTGCAC AGATATATTC CTCGTGAGGG AGACAGGGGA GTTGGAACTG
    GAGGAAGACC TCTACGCCAA GTTGATATTC CTGTACAGGT CACCAGAAAC CATGATCAAG
    TGGACTAGAG AAAAAACAAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526235.1
    CDS1040..9403
    Translation

    Target ORF information:

    RefSeq Version XM_006526172.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526172.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGAAGATGAG GAGCTCCAAG ATGTACAAGT GGAAGGGGAG
    CCCACAGAGA AGGAAGAAGA AGAGGAAGAG GAAATAAAGG AAGAAAGGCA TGAGGTAAAG
    AAGGAAGAGG AAGAGGAAGT GGAGGAAGAT GATGACCAGG ACATCATGAA GGTGCTGGGT
    AATCTGGTGG TGGCCCTCTT CATCAAGTAT TGGATCTACG TCTGCGGGGG CATGTTCTTT
    TTTGTCAGCT TTGAGGGGAA AATCGTCATG TACAAAATCA TCTACATGGT GCTGTTCCTG
    TTCTGCGTGG CCTTGTACCA GGTGCACTAC GAGTGGTGGA GGAAGATCCT GAAGTACTTC
    TGGATGTCGG TGGTCATTTA TACCATGTTG GTGCTCATCT TCATATACAC TTACCAGTTT
    GAAAACTTCC CAGGCCTGTG GCAAAACATG ACCGGATTAA AGAAAGAAAA GCTCGAGGAC
    CTCGGCCTGA AGCAGTTCAC GGTCGCTGAA CTGTTCACTC GCATATTCAT CCCCACCTCC
    TTTCTACTCG TCTGCATCCT ACACCTCCAC TACTTCCACG ATCGGTTCCT GGAACTTACA
    GACCTCAAGT CCATCCCCAG CAAAGAAGAC AACACCATCT ACAGGTTGGC CCACCCTGAA
    GGAAGTCTTC CAGACTTAGC CATCATGAAT ATGACGGCCA GCCTTGATAA GCCTGAGGTG
    CAGAAGTTGG CAGAGTCTGG GGAGGAGAGG CCAGAAGAGT GTGTCAAGAA GACGGAGAAG
    GGTGAGGCTG GGAAAGACAG TGATGAGTCT GAGGAGGAGG AAGATGAAGA AGAGGAATCC
    GAGGAAGAAG AAAGCTCAGA CCTCAGGAAC AAGTGGCACC TGGTGATTGA CCGGCTCACT
    GTGCTCTTTC TAAAATTCCT GGAGTATTTC CACAAGTTGC AGGTGTTTAT GTGGTGGATC
    TTGGAGCTGC ACATCATCAA AATCGTCTCA TCGTACATTA TCTGGGTGAC TGTGAAAGAG
    GTGTCTCTGT TCAACTATGT ATTTTTGATT TCTTGGGCAT TTGCTCTGCC ATATGCCAAA
    CTCCGTCGTG CGGCTTCCAG TGTCTGCACT GTCTGGACAT GTGTGATCAT CGTCTGCAAA
    ATGTTGTACC AGCTGCAAAC CATTAAACCT GAGAACTTTT CTGTCAACTG CTCCTTGCCC
    AATGAAAATC AAACCAACAT TCCCCTTCAT GAGTTGAACA AGTCTCTTCT CTACAGTGCC
    CCTGTTGATC CCACTGAGTG GGTAGGCCTG AGGAAGTCCT CTCCTTTGCT CGTCTACCTG
    AGGAACAACC TCCTGATGCT GGCGATTTTG GCCTTTGAAG TCACCGTTTA CCGCCATCAG
    GAGTACTATC GAGGCCGAAA CAACCTGACT GCTCCAGTGT CTAAAACTAT CTTTCATGAC
    ATCACAAGGT TACATCTAGA TGATGGGCTT ATCAATTGTG CCAAATATTT TGTAAATTAC
    TTCTTCTACA AGTTTGGCCT AGAGACCTGT TTCCTAATGT CCGTGAATGT AATTGGACAG
    CGAATGGACT TCTACGCCAT GATTCATGCC TGTTGGTTGA TCGGTGTCTT GTATCGACGC
    AGAAGAAAAG CCATTGCCGA GGTCTGGCCC AAGTACTGCT GCTTCCTGGC TTGCATCATC
    ACCTTCCAGT ACTTCGTCTG CATCGGCATC CCCCCTGCCC CTTGCCGAGA TTATCCATGG
    AGATTTAAGG GAGCCTACTT CAATGATAAC ATTATCAAGT GGCTGTACTT CCCGGATTTC
    ATTGTGCGGC CCAACCCCGT GTTTCTTGTC TATGACTTCA TGCTGCTCCT GTGTGCTTCC
    TTACAACGAC AGATCTTTGA GGACGAAAAC AAGGCAGCCG TGCGCATCAT GGCCGGGGAC
    AATGTCGAGA TCTGCATGAA CCTTGATGCA GCCTCGTTCA GCCAGCATAA CCCTGTGCCA
    GATTTCATTC ACTGCAGATC TTACTTGGAC ATGTCCAAAG TGATCATCTT CAGCTACCTC
    TTCTGGTTCG TCCTCACCAT CATCTTCATT ACGGGAACAA CCAGGATCAG CATATTTTGC
    ATGGGCTACT TGGTGGCCTG TTTCTATTTC CTGCTCTTTG GAGGGGATCT GCTGCTGAAG
    CCCATCAAGA GCATCCTTCG TTACTGGGAC TGGCTGATTG CATACAACGT GTTTGTGATC
    ACCATGAAGA ATATACTGTC AATAGGAGCC TGTGGATACA TTGGCGCACT AGTAAGAAAC
    AGCTGCTGGT TGATCCAAGC GTTCAGCCTG GCCTGCACCG TGAAAGGCTA TCAGATGCCT
    GAAGATGACT CCAGATGCAA GCTGCCCAGT GGGGAGGCAG GGATCATCTG GGACAGCATC
    TGCTTTGCGT TTCTGCTGCT GCAGAGGAGA GTCTTCATGA GCTACTACTT CCTGCATGTC
    GTGGCTGACA TTAAAGCATC CCAGATCCTG GCATCCAGAG GGGCCGAACT TTTCCAGGCT
    ACAATTGTGA AAGCTGTAAA GGCAAGAATT GAAGAAGAGA AAAAATCCAT GGACCAGCTG
    AAGAGACAGA TGGATCGCAT CAAAGCCCGG CAACAAAAGT ACAAAAAGGG TAAGGAGAGG
    ATGCTGAGCT TGACCCAGGA GTCAGGGGAA GGCCAGGACA TCCAGAAAGT CTCAGAAGAA
    GATGATGAAA GAGAAGCAGA CAAACAGAAA GCCAAGGGCA AAAAAAAGCA GTGGTGGCGG
    CCTTGGGTTG ATCATGCTTC CATGGTCAGG AGTGGAGATT ATTATTTGTT TGAAACGGAT
    AGTGAAGAGG AGGAAGAAGA GGAACTTAAA AAGGAGGATG AAGAACCCCC ACGGAAGTCA
    GCATTCCAGA GAGCTATTGG GAAGTTTGCC TCAGCCATTT TAGCCTTGCC AAAGTCTGTC
    ATTAAACTTC CCAAAACTAT TCTTCAGTAT TTAATCAGAG CTGCAAAGTT CGTCTACCAA
    GCCTGGATTA CTGATCCTAA AACGGCACTC CGACAGAGGA GGAAAGAGAA GAAAAAGTTG
    GCCAGAGAAG AGCAGAAGGA GAGACGCAAA GGATCTGGGG ACGGTCCAGT GGAATGGGAA
    GATCGAGAGG ATGAGCCAGT CAAAAAGAAA TCTGATGGAC CAGATAATAT CATCAAGAGG
    ATATTTAATA TCCTGAAGTT CACATGGGTT CTGTTCCTGG CCACAGTGGA TAGTTTCACC
    ACTTGGCTCA ACTCCATCTC AAGGGAACAC ATCGACATAT CCACAGTCCT GCGGATTGAA
    AGATGCATGC TGACTCGAGA GATAAAAAAG GGCAATGTTC CAACACGGGA AAGCATCCAC
    ATGTACTACC AGAACCACAT TATGAACCTT TCCCGAGAAT CCGGCCTGGA CACAATCGAC
    GAGCACTCGG GGGCTGGCTC CAGGGCCCAG GCAGCACATA GGATGGATAG CTTAGATTCC
    CGGGACAGTA TCTCCAGCGA GCCCACACAG TGTACCATGC TGTATTCGCG CCAAGGAACT
    ACGGAAACCA TCGAGGAGGT GGAGGCTGAA GCTGAGGAGG AGGTGGTAGA AGGTCTGGAG
    CCTGAGCTCC ATGATGCAGA AGAAAAGGAA TACGCAGCTG AATATGAAGC AGGGGTAGAG
    GAGATCAGCC TCACTCCAGA TGAGGAATTG CCTCAGTTCT CTACAGATGA CTGTGATGTG
    GAGGCGCCGC CCTCTTACAG CAAGGCTGTC AGCTTTGAGC ATCTATCGTT TGCGTCGCAA
    GATGACTCTG GGGCCAAGAA CCACATGGTG GTCAGTCCTG ATGACAGCCG TACAGACAAG
    CTGGAGTCAA GCATCTTACC ACCTCTGACT CATGAGCTGA CAGCCAGCGA TCTGCTGATG
    AGCAAGATGT TCCATGATGA TGAGCTTGAG GAGTCTGAGA AGTTCTACGT GGACCAGCCC
    CGGTTTTTGC TGCTATTCTA TGCCATGTAT AACACCCTGG TGGCCCGCTC AGAAATGGTG
    TGCTATTTCG TGATTATCCT GAACCACATG ACCTCTGCCT CCATCATCAC TCTCCTGTTG
    CCCATCTTGA TCTTCCTCTG GGCCATGCTG TCTGTTCCCA GGCCCAGCCG ACGCTTCTGG
    ATGATGGCCA TCGTCTACAC TGAGGTGGCG ATCGTAGTGA AATACTTCTT CCAGTTCGGC
    TTCTTTCCCT GGAATAAGGA CTTGGAAATA TACAAGGAAA GGCCCTACTT TCCACCGAAC
    ATCATCGGGG TGGAGAAGAA GGAGGGCTAT GTTCTTTATG ACCTCATTCA GCTGCTGGCT
    CTATTCTTCC ATCGCTCCAT TCTGAAGTGT CATGGTCTAT GGGACGAGGA TGACATTGTT
    GACAGTAACA CAGACAAGGA AGGATCGGAT GACGAGCTGT CCCTCGATCA GGGCAGAAGA
    GGCTCCTCAG ACTCACTGAA GTCTATCAAC CTGGCTGCCT CTGTAGAGTC TGTCCACGTG
    ACCTTCCCCG AGCAGCCAGC GGCCATCAGG AGAAAACGCT CCTGCAGTAG CTCTCAGATC
    TCCCCACGGT CCAGCTTCTC TTCAAATAGG TCCAAAAGAG GCAGCACAAG CACCCGGAAC
    AGTAGCCAGA AAGGAAGCAG TGTTTTAAGC CTTAAACAGA AAAGCAAACG AGAACTTTAC
    ATGGAAAAGC TTCAAGAGCA TTTAATCAAA GCAAAGGCGT TTACCATAAA GAAGACTCTA
    CAGATCTATG TCCCCATTCG GCAATTCTTC TATGACCTCA TCCACCCAGA CTACAGTGCT
    GTGACTGATG TATACGTGCT GATGTTTCTG GCTGACACTG TAGACTTCAT TATCATCGTC
    TTTGGATTCT GGGCCTTTGG GAAACACTCG GCAGCTGCAG ATATCACCTC TTCTCTATCA
    GAAGACCAGG TCCCCGGGCC ATTCCTGGTG ATGGTCCTCA TTCAGTTTGG AACCATGGTG
    GTGGACCGTG CACTCTACCT CAGGAAGACT GTGCTGGGCA AGGTCATCTT CCAGGTCATC
    CTCGTGTTTG GGATTCACTT CTGGATGTTC TTCATCTTAC CCGGTGTGAC TGAGAGGAAA
    TTCAGCCAGA ACCTGGTGGC ACAGCTTTGG TACTTTGTGA AGTGTGTGTA CTTTGGGCTG
    TCTGCCTATC AAATCCGCTG TGGCTACCCA ACCCGTGTGC TCGGGAACTT CCTCACAAAG
    AGCTACAATT ACGTCAACCT CTTCTTGTTT CAAGGGTTCC GCCTGGTCCC ATTTCTGACT
    GAGCTGCGGG CTGTGATGGA TTGGGTGTGG ACAGATACAA CCCTGAGCCT CTCCAGCTGG
    ATTTGTGTGG AGGACATTTA CGCGCACATT TTTATCTTAA AGTGCTGGCG AGAGTCGGAA
    AAGAGATACC CTCAACCCAG GGGTCAGAAG AAGAAGAAAG CCGTGAAGTA CGGCATGGGC
    GGCATGATCA TCGTCCTTCT CATCTGTATC GTCTGGTTTC CTCTGCTCTT CATGTCTTTG
    ATCAAATCTG TCGCAGGGGT TATCAACCAA CCCCTGGATG TGTCCGTCAC AATTACCCTG
    GGAGGTTATC AGCCTATCTT CACAATGAGC GCCCAGCAAA GCCAGCTGAA AGTTATGGAT
    AATTCAAAGT ATAATGAATT TCTAAAATCT TTCGGCCCCA ACAGTGGCGC AATGCAATTT
    CTGGAGAACT ATGAAAGGGA AGACGTAACA GTAGCAGAAC TGGAAGGGAA CTCGAATTCT
    TTGTGGACCA TCAGCCCTCC CAGTAAGCAG AAAATGATAC AGGAGCTCAC CGACCCCAAT
    AGTTGCTTCT CTGTTGTTTT TTCATGGAGT ATCCAGAGAA ACATGACTTT GGGTGCAAAA
    GCCGAAATAG CAACGGATAA GCTTTCTTTC CCTCTTGCGG TCGCAACACG GAATAGCATA
    GCTAAAATGA TTGCTGGCAA TGACACAGAA AGTTCAAACA CGCCAGTGAC AATAGAGAAG
    ATCTACCCAT ACTATGTGAA GGCACCCAGC GATTCAAACT CAAAGCCTAT AAAACAGCTT
    CTGTCTGAAA ATAATTTCAT GAATATCACC ATCATTTTGT TCAGAGACAA TGTCACAAAG
    TCCAATAGTG AGTGGTGGGT TCTCAACCTG ACCGGAAGTA GGATATTCAA CCAGGGGTCC
    CAAGCCTTGG AACTGGTGGT CTTCAATGAC AAAGTCAGCC CTCCAAGTCT AGGCTTCTTG
    GCTGGCTATG GTATCATGGG ATTGTATGCA TCTGTTGTCC TTGTAATTGG GAAATTTGTT
    CGTGAGTTCT TCAGTGGGAT CTCTCATTCC ATCATGTTTG AAGAGCTTCC AAATGTGGAC
    AGAATCTTGA AGTTGTGCAC AGATATATTC CTCGTGAGGG AGACAGGGGA GTTGGAACTG
    GAGGAAGACC TCTACGCCAA GTTGATATTC CTGTACAGGT CACCAGAAAC CATGATCAAG
    TGGACTAGAG AAAAAACAAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54739
    Clone ID Related Accession (Same CDS sequence) XM_006526173.3 , XM_006526173.4
    Accession Version XM_006526173.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGATGAGGAG CTCCAAGATG TACAAGTGGA AGGGGAGCCC
    ACAGAGAAGG AAGAAGAAGA GGAAGAGGAA ATAAAGGAAG AAAGGCATGA GGTAAAGAAG
    GAAGAGGAAG AGGAAGTGGA GGAAGATGAT GACCAGGACA TCATGAAGGT GCTGGGTAAT
    CTGGTGGTGG CCCTCTTCAT CAAGTATTGG ATCTACGTCT GCGGGGGCAT GTTCTTTTTT
    GTCAGCTTTG AGGGGAAAAT CGTCATGTAC AAAATCATCT ACATGGTGCT GTTCCTGTTC
    TGCGTGGCCT TGTACCAGGT GCACTACGAG TGGTGGAGGA AGATCCTGAA GTACTTCTGG
    ATGTCGGTGG TCATTTATAC CATGTTGGTG CTCATCTTCA TATACACTTA CCAGTTTGAA
    AACTTCCCAG GCCTGTGGCA AAACATGACC GGATTAAAGA AAGAAAAGCT CGAGGACCTC
    GGCCTGAAGC AGTTCACGGT CGCTGAACTG TTCACTCGCA TATTCATCCC CACCTCCTTT
    CTACTCGTCT GCATCCTACA CCTCCACTAC TTCCACGATC GGTTCCTGGA ACTTACAGAC
    CTCAAGTCCA TCCCCAGCAA AGAAGACAAC ACCATCTACA GGTTGGCCCA CCCTGAAGGA
    AGTCTTCCAG ACTTAGCCAT CATGAATATG ACGGCCAGCC TTGATAAGCC TGAGGTGCAG
    AAGTTGGCAG AGTCTGGGGA GGAGAGGCCA GAAGAGTGTG TCAAGAAGAC GGAGAAGGGT
    GAGGCTGGGA AAGACAGTGA TGAGTCTGAG GAGGAGGAAG ATGAAGAAGA GGAATCCGAG
    GAAGAAGAAA GCTCAGACCT CAGGAACAAG TGGCACCTGG TGATTGACCG GCTCACTGTG
    CTCTTTCTAA AATTCCTGGA GTATTTCCAC AAGTTGCAGG TGTTTATGTG GTGGATCTTG
    GAGCTGCACA TCATCAAAAT CGTCTCATCG TACATTATCT GGGTGACTGT GAAAGAGGTG
    TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCATTTG CTCTGCCATA TGCCAAACTC
    CGTCGTGCGG CTTCCAGTGT CTGCACTGTC TGGACATGTG TGATCATCGT CTGCAAAATG
    TTGTACCAGC TGCAAACCAT TAAACCTGAG AACTTTTCTG TCAACTGCTC CTTGCCCAAT
    GAAAATCAAA CCAACATTCC CCTTCATGAG TTGAACAAGT CTCTTCTCTA CAGTGCCCCT
    GTTGATCCCA CTGAGTGGGT AGGCCTGAGG AAGTCCTCTC CTTTGCTCGT CTACCTGAGG
    AACAACCTCC TGATGCTGGC GATTTTGGCC TTTGAAGTCA CCGTTTACCG CCATCAGGAG
    TACTATCGAG GCCGAAACAA CCTGACTGCT CCAGTGTCTA AAACTATCTT TCATGACATC
    ACAAGGTTAC ATCTAGATGA TGGGCTTATC AATTGTGCCA AATATTTTGT AAATTACTTC
    TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCCG TGAATGTAAT TGGACAGCGA
    ATGGACTTCT ACGCCATGAT TCATGCCTGT TGGTTGATCG GTGTCTTGTA TCGACGCAGA
    AGAAAAGCCA TTGCCGAGGT CTGGCCCAAG TACTGCTGCT TCCTGGCTTG CATCATCACC
    TTCCAGTACT TCGTCTGCAT CGGCATCCCC CCTGCCCCTT GCCGAGATTA TCCATGGAGA
    TTTAAGGGAG CCTACTTCAA TGATAACATT ATCAAGTGGC TGTACTTCCC GGATTTCATT
    GTGCGGCCCA ACCCCGTGTT TCTTGTCTAT GACTTCATGC TGCTCCTGTG TGCTTCCTTA
    CAACGACAGA TCTTTGAGGA CGAAAACAAG GCAGCCGTGC GCATCATGGC CGGGGACAAT
    GTCGAGATCT GCATGAACCT TGATGCAGCC TCGTTCAGCC AGCATAACCC TGTGCCAGAT
    TTCATTCACT GCAGATCTTA CTTGGACATG TCCAAAGTGA TCATCTTCAG CTACCTCTTC
    TGGTTCGTCC TCACCATCAT CTTCATTACG GGAACAACCA GGATCAGCAT ATTTTGCATG
    GGCTACTTGG TGGCCTGTTT CTATTTCCTG CTCTTTGGAG GGGATCTGCT GCTGAAGCCC
    ATCAAGAGCA TCCTTCGTTA CTGGGACTGG CTGATTGCAT ACAACGTGTT TGTGATCACC
    ATGAAGAATA TACTGTCAAT AGGAGCCTGT GGATACATTG GCGCACTAGT AAGAAACAGC
    TGCTGGTTGA TCCAAGCGTT CAGCCTGGCC TGCACCGTGA AAGGCTATCA GATGCCTGAA
    GATGACTCCA GATGCAAGCT GCCCAGTGGG GAGGCAGGGA TCATCTGGGA CAGCATCTGC
    TTTGCGTTTC TGCTGCTGCA GAGGAGAGTC TTCATGAGCT ACTACTTCCT GCATGTCGTG
    GCTGACATTA AAGCATCCCA GATCCTGGCA TCCAGAGGGG CCGAACTTTT CCAGGCTACA
    ATTGTGAAAG CTGTAAAGGC AAGAATTGAA GAAGAGAAAA AATCCATGGA CCAGCTGAAG
    AGACAGATGG ATCGCATCAA AGCCCGGCAA CAAAAGTACA AAAAGGGTAA GGAGAGGATG
    CTGAGCTTGA CCCAGGAGTC AGGGGAAGGC CAGGACATCC AGAAAGTCTC AGAAGAAGAT
    GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAGGGCAAAA AAAAGCAGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTTAAAAAG GAGGATGAAG AACCCCCACG GAAGTCAGCA
    TTCCAGTTCG TCTACCAAGC CTGGATTACT GATCCTAAAA CGGCACTCCG ACAGAGGAGG
    AAAGAGAAGA AAAAGTTGGC CAGAGAAGAG CAGAAGGAGA GACGCAAAGG ATCTGGGGAC
    GGTCCAGTGG AATGGGAAGA TCGAGAGGAT GAGCCAGTCA AAAAGAAATC TGATGGACCA
    GATAATATCA TCAAGAGGAT ATTTAATATC CTGAAGTTCA CATGGGTTCT GTTCCTGGCC
    ACAGTGGATA GTTTCACCAC TTGGCTCAAC TCCATCTCAA GGGAACACAT CGACATATCC
    ACAGTCCTGC GGATTGAAAG ATGCATGCTG ACTCGAGAGA TAAAAAAGGG CAATGTTCCA
    ACACGGGAAA GCATCCACAT GTACTACCAG AACCACATTA TGAACCTTTC CCGAGAATCC
    GGCCTGGACA CAATCGACGA GCACTCGGGG GCTGGCTCCA GGGCCCAGGC AGCACATAGG
    ATGGATAGCT TAGATTCCCG GGACAGTATC TCCAGCGAGC CCACACAGTG TACCATGCTG
    TATTCGCGCC AAGGAACTAC GGAAACCATC GAGGAGGTGG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAAG GTCTGGAGCC TGAGCTCCAT GATGCAGAAG AAAAGGAATA CGCAGCTGAA
    TATGAAGCAG GGGTAGAGGA GATCAGCCTC ACTCCAGATG AGGAATTGCC TCAGTTCTCT
    ACAGATGACT GTGATGTGGA GGCGCCGCCC TCTTACAGCA AGGCTGTCAG CTTTGAGCAT
    CTATCGTTTG CGTCGCAAGA TGACTCTGGG GCCAAGAACC ACATGGTGGT CAGTCCTGAT
    GACAGCCGTA CAGACAAGCT GGAGTCAAGC ATCTTACCAC CTCTGACTCA TGAGCTGACA
    GCCAGCGATC TGCTGATGAG CAAGATGTTC CATGATGATG AGCTTGAGGA GTCTGAGAAG
    TTCTACGTGG ACCAGCCCCG GTTTTTGCTG CTATTCTATG CCATGTATAA CACCCTGGTG
    GCCCGCTCAG AAATGGTGTG CTATTTCGTG ATTATCCTGA ACCACATGAC CTCTGCCTCC
    ATCATCACTC TCCTGTTGCC CATCTTGATC TTCCTCTGGG CCATGCTGTC TGTTCCCAGG
    CCCAGCCGAC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT CGTAGTGAAA
    TACTTCTTCC AGTTCGGCTT CTTTCCCTGG AATAAGGACT TGGAAATATA CAAGGAAAGG
    CCCTACTTTC CACCGAACAT CATCGGGGTG GAGAAGAAGG AGGGCTATGT TCTTTATGAC
    CTCATTCAGC TGCTGGCTCT ATTCTTCCAT CGCTCCATTC TGAAGTGTCA TGGTCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCGGATGA CGAGCTGTCC
    CTCGATCAGG GCAGAAGAGG CTCCTCAGAC TCACTGAAGT CTATCAACCT GGCTGCCTCT
    GTAGAGTCTG TCCACGTGAC CTTCCCCGAG CAGCCAGCGG CCATCAGGAG AAAACGCTCC
    TGCAGTAGCT CTCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGGAACAG TAGCCAGAAA GGAAGCAGTG TTTTAAGCCT TAAACAGAAA
    AGCAAACGAG AACTTTACAT GGAAAAGCTT CAAGAGCATT TAATCAAAGC AAAGGCGTTT
    ACCATAAAGA AGACTCTACA GATCTATGTC CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGATGTA TACGTGCTGA TGTTTCTGGC TGACACTGTA
    GACTTCATTA TCATCGTCTT TGGATTCTGG GCCTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CTCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTCCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTGGGCAAG
    GTCATCTTCC AGGTCATCCT CGTGTTTGGG ATTCACTTCT GGATGTTCTT CATCTTACCC
    GGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTTTGGTA CTTTGTGAAG
    TGTGTGTACT TTGGGCTGTC TGCCTATCAA ATCCGCTGTG GCTACCCAAC CCGTGTGCTC
    GGGAACTTCC TCACAAAGAG CTACAATTAC GTCAACCTCT TCTTGTTTCA AGGGTTCCGC
    CTGGTCCCAT TTCTGACTGA GCTGCGGGCT GTGATGGATT GGGTGTGGAC AGATACAACC
    CTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CGCACATTTT TATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GTCAGAAGAA GAAGAAAGCC
    GTGAAGTACG GCATGGGCGG CATGATCATC GTCCTTCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAATCTGTC GCAGGGGTTA TCAACCAACC CCTGGATGTG
    TCCGTCACAA TTACCCTGGG AGGTTATCAG CCTATCTTCA CAATGAGCGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATAA TTCAAAGTAT AATGAATTTC TAAAATCTTT CGGCCCCAAC
    AGTGGCGCAA TGCAATTTCT GGAGAACTAT GAAAGGGAAG ACGTAACAGT AGCAGAACTG
    GAAGGGAACT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTTTGG GTGCAAAAGC CGAAATAGCA ACGGATAAGC TTTCTTTCCC TCTTGCGGTC
    GCAACACGGA ATAGCATAGC TAAAATGATT GCTGGCAATG ACACAGAAAG TTCAAACACG
    CCAGTGACAA TAGAGAAGAT CTACCCATAC TATGTGAAGG CACCCAGCGA TTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCTGAAAAT AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC CGGAAGTAGG
    ATATTCAACC AGGGGTCCCA AGCCTTGGAA CTGGTGGTCT TCAATGACAA AGTCAGCCCT
    CCAAGTCTAG GCTTCTTGGC TGGCTATGGT ATCATGGGAT TGTATGCATC TGTTGTCCTT
    GTAATTGGGA AATTTGTTCG TGAGTTCTTC AGTGGGATCT CTCATTCCAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATCTTGAAG TTGTGCACAG ATATATTCCT CGTGAGGGAG
    ACAGGGGAGT TGGAACTGGA GGAAGACCTC TACGCCAAGT TGATATTCCT GTACAGGTCA
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526236.1
    CDS1039..9300
    Translation

    Target ORF information:

    RefSeq Version XM_006526173.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526173.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGATGAGGAG CTCCAAGATG TACAAGTGGA AGGGGAGCCC
    ACAGAGAAGG AAGAAGAAGA GGAAGAGGAA ATAAAGGAAG AAAGGCATGA GGTAAAGAAG
    GAAGAGGAAG AGGAAGTGGA GGAAGATGAT GACCAGGACA TCATGAAGGT GCTGGGTAAT
    CTGGTGGTGG CCCTCTTCAT CAAGTATTGG ATCTACGTCT GCGGGGGCAT GTTCTTTTTT
    GTCAGCTTTG AGGGGAAAAT CGTCATGTAC AAAATCATCT ACATGGTGCT GTTCCTGTTC
    TGCGTGGCCT TGTACCAGGT GCACTACGAG TGGTGGAGGA AGATCCTGAA GTACTTCTGG
    ATGTCGGTGG TCATTTATAC CATGTTGGTG CTCATCTTCA TATACACTTA CCAGTTTGAA
    AACTTCCCAG GCCTGTGGCA AAACATGACC GGATTAAAGA AAGAAAAGCT CGAGGACCTC
    GGCCTGAAGC AGTTCACGGT CGCTGAACTG TTCACTCGCA TATTCATCCC CACCTCCTTT
    CTACTCGTCT GCATCCTACA CCTCCACTAC TTCCACGATC GGTTCCTGGA ACTTACAGAC
    CTCAAGTCCA TCCCCAGCAA AGAAGACAAC ACCATCTACA GGTTGGCCCA CCCTGAAGGA
    AGTCTTCCAG ACTTAGCCAT CATGAATATG ACGGCCAGCC TTGATAAGCC TGAGGTGCAG
    AAGTTGGCAG AGTCTGGGGA GGAGAGGCCA GAAGAGTGTG TCAAGAAGAC GGAGAAGGGT
    GAGGCTGGGA AAGACAGTGA TGAGTCTGAG GAGGAGGAAG ATGAAGAAGA GGAATCCGAG
    GAAGAAGAAA GCTCAGACCT CAGGAACAAG TGGCACCTGG TGATTGACCG GCTCACTGTG
    CTCTTTCTAA AATTCCTGGA GTATTTCCAC AAGTTGCAGG TGTTTATGTG GTGGATCTTG
    GAGCTGCACA TCATCAAAAT CGTCTCATCG TACATTATCT GGGTGACTGT GAAAGAGGTG
    TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCATTTG CTCTGCCATA TGCCAAACTC
    CGTCGTGCGG CTTCCAGTGT CTGCACTGTC TGGACATGTG TGATCATCGT CTGCAAAATG
    TTGTACCAGC TGCAAACCAT TAAACCTGAG AACTTTTCTG TCAACTGCTC CTTGCCCAAT
    GAAAATCAAA CCAACATTCC CCTTCATGAG TTGAACAAGT CTCTTCTCTA CAGTGCCCCT
    GTTGATCCCA CTGAGTGGGT AGGCCTGAGG AAGTCCTCTC CTTTGCTCGT CTACCTGAGG
    AACAACCTCC TGATGCTGGC GATTTTGGCC TTTGAAGTCA CCGTTTACCG CCATCAGGAG
    TACTATCGAG GCCGAAACAA CCTGACTGCT CCAGTGTCTA AAACTATCTT TCATGACATC
    ACAAGGTTAC ATCTAGATGA TGGGCTTATC AATTGTGCCA AATATTTTGT AAATTACTTC
    TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCCG TGAATGTAAT TGGACAGCGA
    ATGGACTTCT ACGCCATGAT TCATGCCTGT TGGTTGATCG GTGTCTTGTA TCGACGCAGA
    AGAAAAGCCA TTGCCGAGGT CTGGCCCAAG TACTGCTGCT TCCTGGCTTG CATCATCACC
    TTCCAGTACT TCGTCTGCAT CGGCATCCCC CCTGCCCCTT GCCGAGATTA TCCATGGAGA
    TTTAAGGGAG CCTACTTCAA TGATAACATT ATCAAGTGGC TGTACTTCCC GGATTTCATT
    GTGCGGCCCA ACCCCGTGTT TCTTGTCTAT GACTTCATGC TGCTCCTGTG TGCTTCCTTA
    CAACGACAGA TCTTTGAGGA CGAAAACAAG GCAGCCGTGC GCATCATGGC CGGGGACAAT
    GTCGAGATCT GCATGAACCT TGATGCAGCC TCGTTCAGCC AGCATAACCC TGTGCCAGAT
    TTCATTCACT GCAGATCTTA CTTGGACATG TCCAAAGTGA TCATCTTCAG CTACCTCTTC
    TGGTTCGTCC TCACCATCAT CTTCATTACG GGAACAACCA GGATCAGCAT ATTTTGCATG
    GGCTACTTGG TGGCCTGTTT CTATTTCCTG CTCTTTGGAG GGGATCTGCT GCTGAAGCCC
    ATCAAGAGCA TCCTTCGTTA CTGGGACTGG CTGATTGCAT ACAACGTGTT TGTGATCACC
    ATGAAGAATA TACTGTCAAT AGGAGCCTGT GGATACATTG GCGCACTAGT AAGAAACAGC
    TGCTGGTTGA TCCAAGCGTT CAGCCTGGCC TGCACCGTGA AAGGCTATCA GATGCCTGAA
    GATGACTCCA GATGCAAGCT GCCCAGTGGG GAGGCAGGGA TCATCTGGGA CAGCATCTGC
    TTTGCGTTTC TGCTGCTGCA GAGGAGAGTC TTCATGAGCT ACTACTTCCT GCATGTCGTG
    GCTGACATTA AAGCATCCCA GATCCTGGCA TCCAGAGGGG CCGAACTTTT CCAGGCTACA
    ATTGTGAAAG CTGTAAAGGC AAGAATTGAA GAAGAGAAAA AATCCATGGA CCAGCTGAAG
    AGACAGATGG ATCGCATCAA AGCCCGGCAA CAAAAGTACA AAAAGGGTAA GGAGAGGATG
    CTGAGCTTGA CCCAGGAGTC AGGGGAAGGC CAGGACATCC AGAAAGTCTC AGAAGAAGAT
    GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAGGGCAAAA AAAAGCAGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTTAAAAAG GAGGATGAAG AACCCCCACG GAAGTCAGCA
    TTCCAGTTCG TCTACCAAGC CTGGATTACT GATCCTAAAA CGGCACTCCG ACAGAGGAGG
    AAAGAGAAGA AAAAGTTGGC CAGAGAAGAG CAGAAGGAGA GACGCAAAGG ATCTGGGGAC
    GGTCCAGTGG AATGGGAAGA TCGAGAGGAT GAGCCAGTCA AAAAGAAATC TGATGGACCA
    GATAATATCA TCAAGAGGAT ATTTAATATC CTGAAGTTCA CATGGGTTCT GTTCCTGGCC
    ACAGTGGATA GTTTCACCAC TTGGCTCAAC TCCATCTCAA GGGAACACAT CGACATATCC
    ACAGTCCTGC GGATTGAAAG ATGCATGCTG ACTCGAGAGA TAAAAAAGGG CAATGTTCCA
    ACACGGGAAA GCATCCACAT GTACTACCAG AACCACATTA TGAACCTTTC CCGAGAATCC
    GGCCTGGACA CAATCGACGA GCACTCGGGG GCTGGCTCCA GGGCCCAGGC AGCACATAGG
    ATGGATAGCT TAGATTCCCG GGACAGTATC TCCAGCGAGC CCACACAGTG TACCATGCTG
    TATTCGCGCC AAGGAACTAC GGAAACCATC GAGGAGGTGG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAAG GTCTGGAGCC TGAGCTCCAT GATGCAGAAG AAAAGGAATA CGCAGCTGAA
    TATGAAGCAG GGGTAGAGGA GATCAGCCTC ACTCCAGATG AGGAATTGCC TCAGTTCTCT
    ACAGATGACT GTGATGTGGA GGCGCCGCCC TCTTACAGCA AGGCTGTCAG CTTTGAGCAT
    CTATCGTTTG CGTCGCAAGA TGACTCTGGG GCCAAGAACC ACATGGTGGT CAGTCCTGAT
    GACAGCCGTA CAGACAAGCT GGAGTCAAGC ATCTTACCAC CTCTGACTCA TGAGCTGACA
    GCCAGCGATC TGCTGATGAG CAAGATGTTC CATGATGATG AGCTTGAGGA GTCTGAGAAG
    TTCTACGTGG ACCAGCCCCG GTTTTTGCTG CTATTCTATG CCATGTATAA CACCCTGGTG
    GCCCGCTCAG AAATGGTGTG CTATTTCGTG ATTATCCTGA ACCACATGAC CTCTGCCTCC
    ATCATCACTC TCCTGTTGCC CATCTTGATC TTCCTCTGGG CCATGCTGTC TGTTCCCAGG
    CCCAGCCGAC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT CGTAGTGAAA
    TACTTCTTCC AGTTCGGCTT CTTTCCCTGG AATAAGGACT TGGAAATATA CAAGGAAAGG
    CCCTACTTTC CACCGAACAT CATCGGGGTG GAGAAGAAGG AGGGCTATGT TCTTTATGAC
    CTCATTCAGC TGCTGGCTCT ATTCTTCCAT CGCTCCATTC TGAAGTGTCA TGGTCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCGGATGA CGAGCTGTCC
    CTCGATCAGG GCAGAAGAGG CTCCTCAGAC TCACTGAAGT CTATCAACCT GGCTGCCTCT
    GTAGAGTCTG TCCACGTGAC CTTCCCCGAG CAGCCAGCGG CCATCAGGAG AAAACGCTCC
    TGCAGTAGCT CTCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGGAACAG TAGCCAGAAA GGAAGCAGTG TTTTAAGCCT TAAACAGAAA
    AGCAAACGAG AACTTTACAT GGAAAAGCTT CAAGAGCATT TAATCAAAGC AAAGGCGTTT
    ACCATAAAGA AGACTCTACA GATCTATGTC CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGATGTA TACGTGCTGA TGTTTCTGGC TGACACTGTA
    GACTTCATTA TCATCGTCTT TGGATTCTGG GCCTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CTCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTCCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTGGGCAAG
    GTCATCTTCC AGGTCATCCT CGTGTTTGGG ATTCACTTCT GGATGTTCTT CATCTTACCC
    GGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTTTGGTA CTTTGTGAAG
    TGTGTGTACT TTGGGCTGTC TGCCTATCAA ATCCGCTGTG GCTACCCAAC CCGTGTGCTC
    GGGAACTTCC TCACAAAGAG CTACAATTAC GTCAACCTCT TCTTGTTTCA AGGGTTCCGC
    CTGGTCCCAT TTCTGACTGA GCTGCGGGCT GTGATGGATT GGGTGTGGAC AGATACAACC
    CTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CGCACATTTT TATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GTCAGAAGAA GAAGAAAGCC
    GTGAAGTACG GCATGGGCGG CATGATCATC GTCCTTCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAATCTGTC GCAGGGGTTA TCAACCAACC CCTGGATGTG
    TCCGTCACAA TTACCCTGGG AGGTTATCAG CCTATCTTCA CAATGAGCGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATAA TTCAAAGTAT AATGAATTTC TAAAATCTTT CGGCCCCAAC
    AGTGGCGCAA TGCAATTTCT GGAGAACTAT GAAAGGGAAG ACGTAACAGT AGCAGAACTG
    GAAGGGAACT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTTTGG GTGCAAAAGC CGAAATAGCA ACGGATAAGC TTTCTTTCCC TCTTGCGGTC
    GCAACACGGA ATAGCATAGC TAAAATGATT GCTGGCAATG ACACAGAAAG TTCAAACACG
    CCAGTGACAA TAGAGAAGAT CTACCCATAC TATGTGAAGG CACCCAGCGA TTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCTGAAAAT AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC CGGAAGTAGG
    ATATTCAACC AGGGGTCCCA AGCCTTGGAA CTGGTGGTCT TCAATGACAA AGTCAGCCCT
    CCAAGTCTAG GCTTCTTGGC TGGCTATGGT ATCATGGGAT TGTATGCATC TGTTGTCCTT
    GTAATTGGGA AATTTGTTCG TGAGTTCTTC AGTGGGATCT CTCATTCCAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATCTTGAAG TTGTGCACAG ATATATTCCT CGTGAGGGAG
    ACAGGGGAGT TGGAACTGGA GGAAGACCTC TACGCCAAGT TGATATTCCT GTACAGGTCA
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54739
    Clone ID Related Accession (Same CDS sequence) XM_006526173.3 , XM_006526173.4
    Accession Version XM_006526173.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526173.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGATGAGGAG CTCCAAGATG TACAAGTGGA AGGGGAGCCC
    ACAGAGAAGG AAGAAGAAGA GGAAGAGGAA ATAAAGGAAG AAAGGCATGA GGTAAAGAAG
    GAAGAGGAAG AGGAAGTGGA GGAAGATGAT GACCAGGACA TCATGAAGGT GCTGGGTAAT
    CTGGTGGTGG CCCTCTTCAT CAAGTATTGG ATCTACGTCT GCGGGGGCAT GTTCTTTTTT
    GTCAGCTTTG AGGGGAAAAT CGTCATGTAC AAAATCATCT ACATGGTGCT GTTCCTGTTC
    TGCGTGGCCT TGTACCAGGT GCACTACGAG TGGTGGAGGA AGATCCTGAA GTACTTCTGG
    ATGTCGGTGG TCATTTATAC CATGTTGGTG CTCATCTTCA TATACACTTA CCAGTTTGAA
    AACTTCCCAG GCCTGTGGCA AAACATGACC GGATTAAAGA AAGAAAAGCT CGAGGACCTC
    GGCCTGAAGC AGTTCACGGT CGCTGAACTG TTCACTCGCA TATTCATCCC CACCTCCTTT
    CTACTCGTCT GCATCCTACA CCTCCACTAC TTCCACGATC GGTTCCTGGA ACTTACAGAC
    CTCAAGTCCA TCCCCAGCAA AGAAGACAAC ACCATCTACA GGTTGGCCCA CCCTGAAGGA
    AGTCTTCCAG ACTTAGCCAT CATGAATATG ACGGCCAGCC TTGATAAGCC TGAGGTGCAG
    AAGTTGGCAG AGTCTGGGGA GGAGAGGCCA GAAGAGTGTG TCAAGAAGAC GGAGAAGGGT
    GAGGCTGGGA AAGACAGTGA TGAGTCTGAG GAGGAGGAAG ATGAAGAAGA GGAATCCGAG
    GAAGAAGAAA GCTCAGACCT CAGGAACAAG TGGCACCTGG TGATTGACCG GCTCACTGTG
    CTCTTTCTAA AATTCCTGGA GTATTTCCAC AAGTTGCAGG TGTTTATGTG GTGGATCTTG
    GAGCTGCACA TCATCAAAAT CGTCTCATCG TACATTATCT GGGTGACTGT GAAAGAGGTG
    TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCATTTG CTCTGCCATA TGCCAAACTC
    CGTCGTGCGG CTTCCAGTGT CTGCACTGTC TGGACATGTG TGATCATCGT CTGCAAAATG
    TTGTACCAGC TGCAAACCAT TAAACCTGAG AACTTTTCTG TCAACTGCTC CTTGCCCAAT
    GAAAATCAAA CCAACATTCC CCTTCATGAG TTGAACAAGT CTCTTCTCTA CAGTGCCCCT
    GTTGATCCCA CTGAGTGGGT AGGCCTGAGG AAGTCCTCTC CTTTGCTCGT CTACCTGAGG
    AACAACCTCC TGATGCTGGC GATTTTGGCC TTTGAAGTCA CCGTTTACCG CCATCAGGAG
    TACTATCGAG GCCGAAACAA CCTGACTGCT CCAGTGTCTA AAACTATCTT TCATGACATC
    ACAAGGTTAC ATCTAGATGA TGGGCTTATC AATTGTGCCA AATATTTTGT AAATTACTTC
    TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCCG TGAATGTAAT TGGACAGCGA
    ATGGACTTCT ACGCCATGAT TCATGCCTGT TGGTTGATCG GTGTCTTGTA TCGACGCAGA
    AGAAAAGCCA TTGCCGAGGT CTGGCCCAAG TACTGCTGCT TCCTGGCTTG CATCATCACC
    TTCCAGTACT TCGTCTGCAT CGGCATCCCC CCTGCCCCTT GCCGAGATTA TCCATGGAGA
    TTTAAGGGAG CCTACTTCAA TGATAACATT ATCAAGTGGC TGTACTTCCC GGATTTCATT
    GTGCGGCCCA ACCCCGTGTT TCTTGTCTAT GACTTCATGC TGCTCCTGTG TGCTTCCTTA
    CAACGACAGA TCTTTGAGGA CGAAAACAAG GCAGCCGTGC GCATCATGGC CGGGGACAAT
    GTCGAGATCT GCATGAACCT TGATGCAGCC TCGTTCAGCC AGCATAACCC TGTGCCAGAT
    TTCATTCACT GCAGATCTTA CTTGGACATG TCCAAAGTGA TCATCTTCAG CTACCTCTTC
    TGGTTCGTCC TCACCATCAT CTTCATTACG GGAACAACCA GGATCAGCAT ATTTTGCATG
    GGCTACTTGG TGGCCTGTTT CTATTTCCTG CTCTTTGGAG GGGATCTGCT GCTGAAGCCC
    ATCAAGAGCA TCCTTCGTTA CTGGGACTGG CTGATTGCAT ACAACGTGTT TGTGATCACC
    ATGAAGAATA TACTGTCAAT AGGAGCCTGT GGATACATTG GCGCACTAGT AAGAAACAGC
    TGCTGGTTGA TCCAAGCGTT CAGCCTGGCC TGCACCGTGA AAGGCTATCA GATGCCTGAA
    GATGACTCCA GATGCAAGCT GCCCAGTGGG GAGGCAGGGA TCATCTGGGA CAGCATCTGC
    TTTGCGTTTC TGCTGCTGCA GAGGAGAGTC TTCATGAGCT ACTACTTCCT GCATGTCGTG
    GCTGACATTA AAGCATCCCA GATCCTGGCA TCCAGAGGGG CCGAACTTTT CCAGGCTACA
    ATTGTGAAAG CTGTAAAGGC AAGAATTGAA GAAGAGAAAA AATCCATGGA CCAGCTGAAG
    AGACAGATGG ATCGCATCAA AGCCCGGCAA CAAAAGTACA AAAAGGGTAA GGAGAGGATG
    CTGAGCTTGA CCCAGGAGTC AGGGGAAGGC CAGGACATCC AGAAAGTCTC AGAAGAAGAT
    GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAGGGCAAAA AAAAGCAGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTTAAAAAG GAGGATGAAG AACCCCCACG GAAGTCAGCA
    TTCCAGTTCG TCTACCAAGC CTGGATTACT GATCCTAAAA CGGCACTCCG ACAGAGGAGG
    AAAGAGAAGA AAAAGTTGGC CAGAGAAGAG CAGAAGGAGA GACGCAAAGG ATCTGGGGAC
    GGTCCAGTGG AATGGGAAGA TCGAGAGGAT GAGCCAGTCA AAAAGAAATC TGATGGACCA
    GATAATATCA TCAAGAGGAT ATTTAATATC CTGAAGTTCA CATGGGTTCT GTTCCTGGCC
    ACAGTGGATA GTTTCACCAC TTGGCTCAAC TCCATCTCAA GGGAACACAT CGACATATCC
    ACAGTCCTGC GGATTGAAAG ATGCATGCTG ACTCGAGAGA TAAAAAAGGG CAATGTTCCA
    ACACGGGAAA GCATCCACAT GTACTACCAG AACCACATTA TGAACCTTTC CCGAGAATCC
    GGCCTGGACA CAATCGACGA GCACTCGGGG GCTGGCTCCA GGGCCCAGGC AGCACATAGG
    ATGGATAGCT TAGATTCCCG GGACAGTATC TCCAGCGAGC CCACACAGTG TACCATGCTG
    TATTCGCGCC AAGGAACTAC GGAAACCATC GAGGAGGTGG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAAG GTCTGGAGCC TGAGCTCCAT GATGCAGAAG AAAAGGAATA CGCAGCTGAA
    TATGAAGCAG GGGTAGAGGA GATCAGCCTC ACTCCAGATG AGGAATTGCC TCAGTTCTCT
    ACAGATGACT GTGATGTGGA GGCGCCGCCC TCTTACAGCA AGGCTGTCAG CTTTGAGCAT
    CTATCGTTTG CGTCGCAAGA TGACTCTGGG GCCAAGAACC ACATGGTGGT CAGTCCTGAT
    GACAGCCGTA CAGACAAGCT GGAGTCAAGC ATCTTACCAC CTCTGACTCA TGAGCTGACA
    GCCAGCGATC TGCTGATGAG CAAGATGTTC CATGATGATG AGCTTGAGGA GTCTGAGAAG
    TTCTACGTGG ACCAGCCCCG GTTTTTGCTG CTATTCTATG CCATGTATAA CACCCTGGTG
    GCCCGCTCAG AAATGGTGTG CTATTTCGTG ATTATCCTGA ACCACATGAC CTCTGCCTCC
    ATCATCACTC TCCTGTTGCC CATCTTGATC TTCCTCTGGG CCATGCTGTC TGTTCCCAGG
    CCCAGCCGAC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT CGTAGTGAAA
    TACTTCTTCC AGTTCGGCTT CTTTCCCTGG AATAAGGACT TGGAAATATA CAAGGAAAGG
    CCCTACTTTC CACCGAACAT CATCGGGGTG GAGAAGAAGG AGGGCTATGT TCTTTATGAC
    CTCATTCAGC TGCTGGCTCT ATTCTTCCAT CGCTCCATTC TGAAGTGTCA TGGTCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCGGATGA CGAGCTGTCC
    CTCGATCAGG GCAGAAGAGG CTCCTCAGAC TCACTGAAGT CTATCAACCT GGCTGCCTCT
    GTAGAGTCTG TCCACGTGAC CTTCCCCGAG CAGCCAGCGG CCATCAGGAG AAAACGCTCC
    TGCAGTAGCT CTCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGGAACAG TAGCCAGAAA GGAAGCAGTG TTTTAAGCCT TAAACAGAAA
    AGCAAACGAG AACTTTACAT GGAAAAGCTT CAAGAGCATT TAATCAAAGC AAAGGCGTTT
    ACCATAAAGA AGACTCTACA GATCTATGTC CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGATGTA TACGTGCTGA TGTTTCTGGC TGACACTGTA
    GACTTCATTA TCATCGTCTT TGGATTCTGG GCCTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CTCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTCCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTGGGCAAG
    GTCATCTTCC AGGTCATCCT CGTGTTTGGG ATTCACTTCT GGATGTTCTT CATCTTACCC
    GGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTTTGGTA CTTTGTGAAG
    TGTGTGTACT TTGGGCTGTC TGCCTATCAA ATCCGCTGTG GCTACCCAAC CCGTGTGCTC
    GGGAACTTCC TCACAAAGAG CTACAATTAC GTCAACCTCT TCTTGTTTCA AGGGTTCCGC
    CTGGTCCCAT TTCTGACTGA GCTGCGGGCT GTGATGGATT GGGTGTGGAC AGATACAACC
    CTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CGCACATTTT TATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GTCAGAAGAA GAAGAAAGCC
    GTGAAGTACG GCATGGGCGG CATGATCATC GTCCTTCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAATCTGTC GCAGGGGTTA TCAACCAACC CCTGGATGTG
    TCCGTCACAA TTACCCTGGG AGGTTATCAG CCTATCTTCA CAATGAGCGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATAA TTCAAAGTAT AATGAATTTC TAAAATCTTT CGGCCCCAAC
    AGTGGCGCAA TGCAATTTCT GGAGAACTAT GAAAGGGAAG ACGTAACAGT AGCAGAACTG
    GAAGGGAACT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTTTGG GTGCAAAAGC CGAAATAGCA ACGGATAAGC TTTCTTTCCC TCTTGCGGTC
    GCAACACGGA ATAGCATAGC TAAAATGATT GCTGGCAATG ACACAGAAAG TTCAAACACG
    CCAGTGACAA TAGAGAAGAT CTACCCATAC TATGTGAAGG CACCCAGCGA TTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCTGAAAAT AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC CGGAAGTAGG
    ATATTCAACC AGGGGTCCCA AGCCTTGGAA CTGGTGGTCT TCAATGACAA AGTCAGCCCT
    CCAAGTCTAG GCTTCTTGGC TGGCTATGGT ATCATGGGAT TGTATGCATC TGTTGTCCTT
    GTAATTGGGA AATTTGTTCG TGAGTTCTTC AGTGGGATCT CTCATTCCAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATCTTGAAG TTGTGCACAG ATATATTCCT CGTGAGGGAG
    ACAGGGGAGT TGGAACTGGA GGAAGACCTC TACGCCAAGT TGATATTCCT GTACAGGTCA
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006526236.1
    CDS1448..9709
    Translation

    Target ORF information:

    RefSeq Version XM_006526173.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 2 (Piezo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006526173.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTTCGG AAGTGGTGTG CGGGCTGATC TTCAGGCTGC TGCTTCCCAT CTGCCTGGCA 
    GTAGCATGTG CGTTCCGGTA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC
    ATTCCTCTGT TCTCAGAACC AACCAAAGCA ACGATGCAAG GACACACAGG GCGGTTGCTA
    CAGTCCCTGT GCATCACCAG CCTCTCATTT CTGCTGCTTC ACATCATTTT CCACATCACA
    CTGGCCAGTC TGGAAGCTCA ACACCGCATC ACACCTGCTT ACAACTGCTC AACATGGGAA
    AAGACCTTCC GGCAGATTGG CTTTGAAAGC TTGAAGGGAG CTGATGCCGG CAATGGCATC
    AGGGTATTTG TGCCTGATAT CGGGATGTTC ATTGCAAGTC TGACCATCTG GCTGGTCTGC
    AGAACCATTG TTAAGAAACC AGACACAGAA GAAATAGCCC AATTGAATTC TGAGTGTGAA
    AATGAAGAAT TGGCTGGAGG AGAAAAGATG GATTCAGAGG AGGCGCTGAT TTATGAAGAG
    GACTTAGATG GAGAAGAAGG CATGGAAGGA GAGCTAGAAG AAAGCACAAA ACTAAAAATA
    CTTCGCAGGT TCGCCTCTGT CGCCTCCAAA CTGAAGGAGT TCATTGGCAA TATGATCACC
    ACGGCGGGCA AGGTCGTGGT GACCATCCTA CTGGGCTCCT CGGGCATGAT GCTGCCATCT
    TTGACCTCGG CTGTGTACTT CTTTGTATTT CTGGGCCTGT GTACCTGGTG GTCCTGGTGC
    CGAACTTTCG ACCCATTGCT GTTCGGCTGC CTCTGTGTCC TGCTGGCAAT CTTCACTGCT
    GGGCACCTGA TTGGACTTTA TCTGTACCAG TTCCAATTCT TCCAAGAAGC GGTCCCGCCC
    AATGACTACT ATGCAAGGTT GTTTGGGATC AAGTCCGTTA TCCAGACAGA CTGTGCCAGC
    ACCTGGAAGA TCATAGTAAA CCCAGACCTG TCCTGGTATC ACCATGCCAA CCCCATCCTC
    CTGCTGGTGA TGTACTATAC GCTGGCCACG CTGATTCGCA TCTGGCTACA AGAGCCTCTT
    GTGCAAGAGG AGATGGCGAA GGAAGATGAG GGTGCCCTGG ATTGCAGCTC CAATCAGAAC
    ACGGCAGAGA GGAGGCGGAG CCTGTGGTAT GCAACCCAGT ACCCAACAGA TGAGAGAAAA
    CTTTTATCCA TGACCCAAGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACCGTGAAT
    GGCAACCCTG TGGACTACCA CACCATCCAC CCAAGCCTGC CCATAGAGAA TGGCCCTGCC
    AAGACGGACC TGTACACCAC TCCCCAGTAC CGGTGGGAGC CTTCGGAGGA GTCCTCAGAA
    AAGAAAGAGG AAGAAGAGGA CAAGAGAGAA GACTCCGAAG GGGAAGGGAG CCAGGAGGAG
    AAAAGGAGTG TGAGAATGCA CGCCATGGTC GCCGTGTTCC AGTTCATCAT GAAGCAGAGT
    TACATCTGTG CCCTCATCGC CATGATGGCA TGGAGCATCA CCTACCACAG CTGGCTGACT
    TTCGTGCTGT TGATCTGGTC CTGCACCCTC TGGATGATTC GCAACAGGAG GAAGTATGCC
    ATGATCAGTT CTCCTTTCAT GGTCGTCTAT GCGAATCTGC TGCTGGTGCT GCAGTACATA
    TGGAGCTTCG AGCTCCCCGA GATTAAGAAG GTCCCCGGGT TTCTAGAAAA GAAAGAGCCG
    GGAGAACTTG CTTCAAAGAT TCTCTTCACC ATCACGTTCT GGCTGCTCCT GAGGCAGCAT
    CTCACAGAGC AGAAGGCCCT GAGGGAAAAG GAAGCACTTC TTTCTGAAGT CAAAATTGGA
    AGTCAAGAAC TTGAAGAAAA GGATGAGGAG CTCCAAGATG TACAAGTGGA AGGGGAGCCC
    ACAGAGAAGG AAGAAGAAGA GGAAGAGGAA ATAAAGGAAG AAAGGCATGA GGTAAAGAAG
    GAAGAGGAAG AGGAAGTGGA GGAAGATGAT GACCAGGACA TCATGAAGGT GCTGGGTAAT
    CTGGTGGTGG CCCTCTTCAT CAAGTATTGG ATCTACGTCT GCGGGGGCAT GTTCTTTTTT
    GTCAGCTTTG AGGGGAAAAT CGTCATGTAC AAAATCATCT ACATGGTGCT GTTCCTGTTC
    TGCGTGGCCT TGTACCAGGT GCACTACGAG TGGTGGAGGA AGATCCTGAA GTACTTCTGG
    ATGTCGGTGG TCATTTATAC CATGTTGGTG CTCATCTTCA TATACACTTA CCAGTTTGAA
    AACTTCCCAG GCCTGTGGCA AAACATGACC GGATTAAAGA AAGAAAAGCT CGAGGACCTC
    GGCCTGAAGC AGTTCACGGT CGCTGAACTG TTCACTCGCA TATTCATCCC CACCTCCTTT
    CTACTCGTCT GCATCCTACA CCTCCACTAC TTCCACGATC GGTTCCTGGA ACTTACAGAC
    CTCAAGTCCA TCCCCAGCAA AGAAGACAAC ACCATCTACA GGTTGGCCCA CCCTGAAGGA
    AGTCTTCCAG ACTTAGCCAT CATGAATATG ACGGCCAGCC TTGATAAGCC TGAGGTGCAG
    AAGTTGGCAG AGTCTGGGGA GGAGAGGCCA GAAGAGTGTG TCAAGAAGAC GGAGAAGGGT
    GAGGCTGGGA AAGACAGTGA TGAGTCTGAG GAGGAGGAAG ATGAAGAAGA GGAATCCGAG
    GAAGAAGAAA GCTCAGACCT CAGGAACAAG TGGCACCTGG TGATTGACCG GCTCACTGTG
    CTCTTTCTAA AATTCCTGGA GTATTTCCAC AAGTTGCAGG TGTTTATGTG GTGGATCTTG
    GAGCTGCACA TCATCAAAAT CGTCTCATCG TACATTATCT GGGTGACTGT GAAAGAGGTG
    TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCATTTG CTCTGCCATA TGCCAAACTC
    CGTCGTGCGG CTTCCAGTGT CTGCACTGTC TGGACATGTG TGATCATCGT CTGCAAAATG
    TTGTACCAGC TGCAAACCAT TAAACCTGAG AACTTTTCTG TCAACTGCTC CTTGCCCAAT
    GAAAATCAAA CCAACATTCC CCTTCATGAG TTGAACAAGT CTCTTCTCTA CAGTGCCCCT
    GTTGATCCCA CTGAGTGGGT AGGCCTGAGG AAGTCCTCTC CTTTGCTCGT CTACCTGAGG
    AACAACCTCC TGATGCTGGC GATTTTGGCC TTTGAAGTCA CCGTTTACCG CCATCAGGAG
    TACTATCGAG GCCGAAACAA CCTGACTGCT CCAGTGTCTA AAACTATCTT TCATGACATC
    ACAAGGTTAC ATCTAGATGA TGGGCTTATC AATTGTGCCA AATATTTTGT AAATTACTTC
    TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCCG TGAATGTAAT TGGACAGCGA
    ATGGACTTCT ACGCCATGAT TCATGCCTGT TGGTTGATCG GTGTCTTGTA TCGACGCAGA
    AGAAAAGCCA TTGCCGAGGT CTGGCCCAAG TACTGCTGCT TCCTGGCTTG CATCATCACC
    TTCCAGTACT TCGTCTGCAT CGGCATCCCC CCTGCCCCTT GCCGAGATTA TCCATGGAGA
    TTTAAGGGAG CCTACTTCAA TGATAACATT ATCAAGTGGC TGTACTTCCC GGATTTCATT
    GTGCGGCCCA ACCCCGTGTT TCTTGTCTAT GACTTCATGC TGCTCCTGTG TGCTTCCTTA
    CAACGACAGA TCTTTGAGGA CGAAAACAAG GCAGCCGTGC GCATCATGGC CGGGGACAAT
    GTCGAGATCT GCATGAACCT TGATGCAGCC TCGTTCAGCC AGCATAACCC TGTGCCAGAT
    TTCATTCACT GCAGATCTTA CTTGGACATG TCCAAAGTGA TCATCTTCAG CTACCTCTTC
    TGGTTCGTCC TCACCATCAT CTTCATTACG GGAACAACCA GGATCAGCAT ATTTTGCATG
    GGCTACTTGG TGGCCTGTTT CTATTTCCTG CTCTTTGGAG GGGATCTGCT GCTGAAGCCC
    ATCAAGAGCA TCCTTCGTTA CTGGGACTGG CTGATTGCAT ACAACGTGTT TGTGATCACC
    ATGAAGAATA TACTGTCAAT AGGAGCCTGT GGATACATTG GCGCACTAGT AAGAAACAGC
    TGCTGGTTGA TCCAAGCGTT CAGCCTGGCC TGCACCGTGA AAGGCTATCA GATGCCTGAA
    GATGACTCCA GATGCAAGCT GCCCAGTGGG GAGGCAGGGA TCATCTGGGA CAGCATCTGC
    TTTGCGTTTC TGCTGCTGCA GAGGAGAGTC TTCATGAGCT ACTACTTCCT GCATGTCGTG
    GCTGACATTA AAGCATCCCA GATCCTGGCA TCCAGAGGGG CCGAACTTTT CCAGGCTACA
    ATTGTGAAAG CTGTAAAGGC AAGAATTGAA GAAGAGAAAA AATCCATGGA CCAGCTGAAG
    AGACAGATGG ATCGCATCAA AGCCCGGCAA CAAAAGTACA AAAAGGGTAA GGAGAGGATG
    CTGAGCTTGA CCCAGGAGTC AGGGGAAGGC CAGGACATCC AGAAAGTCTC AGAAGAAGAT
    GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAGGGCAAAA AAAAGCAGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT
    GAAGAGGAGG AAGAAGAGGA ACTTAAAAAG GAGGATGAAG AACCCCCACG GAAGTCAGCA
    TTCCAGTTCG TCTACCAAGC CTGGATTACT GATCCTAAAA CGGCACTCCG ACAGAGGAGG
    AAAGAGAAGA AAAAGTTGGC CAGAGAAGAG CAGAAGGAGA GACGCAAAGG ATCTGGGGAC
    GGTCCAGTGG AATGGGAAGA TCGAGAGGAT GAGCCAGTCA AAAAGAAATC TGATGGACCA
    GATAATATCA TCAAGAGGAT ATTTAATATC CTGAAGTTCA CATGGGTTCT GTTCCTGGCC
    ACAGTGGATA GTTTCACCAC TTGGCTCAAC TCCATCTCAA GGGAACACAT CGACATATCC
    ACAGTCCTGC GGATTGAAAG ATGCATGCTG ACTCGAGAGA TAAAAAAGGG CAATGTTCCA
    ACACGGGAAA GCATCCACAT GTACTACCAG AACCACATTA TGAACCTTTC CCGAGAATCC
    GGCCTGGACA CAATCGACGA GCACTCGGGG GCTGGCTCCA GGGCCCAGGC AGCACATAGG
    ATGGATAGCT TAGATTCCCG GGACAGTATC TCCAGCGAGC CCACACAGTG TACCATGCTG
    TATTCGCGCC AAGGAACTAC GGAAACCATC GAGGAGGTGG AGGCTGAAGC TGAGGAGGAG
    GTGGTAGAAG GTCTGGAGCC TGAGCTCCAT GATGCAGAAG AAAAGGAATA CGCAGCTGAA
    TATGAAGCAG GGGTAGAGGA GATCAGCCTC ACTCCAGATG AGGAATTGCC TCAGTTCTCT
    ACAGATGACT GTGATGTGGA GGCGCCGCCC TCTTACAGCA AGGCTGTCAG CTTTGAGCAT
    CTATCGTTTG CGTCGCAAGA TGACTCTGGG GCCAAGAACC ACATGGTGGT CAGTCCTGAT
    GACAGCCGTA CAGACAAGCT GGAGTCAAGC ATCTTACCAC CTCTGACTCA TGAGCTGACA
    GCCAGCGATC TGCTGATGAG CAAGATGTTC CATGATGATG AGCTTGAGGA GTCTGAGAAG
    TTCTACGTGG ACCAGCCCCG GTTTTTGCTG CTATTCTATG CCATGTATAA CACCCTGGTG
    GCCCGCTCAG AAATGGTGTG CTATTTCGTG ATTATCCTGA ACCACATGAC CTCTGCCTCC
    ATCATCACTC TCCTGTTGCC CATCTTGATC TTCCTCTGGG CCATGCTGTC TGTTCCCAGG
    CCCAGCCGAC GCTTCTGGAT GATGGCCATC GTCTACACTG AGGTGGCGAT CGTAGTGAAA
    TACTTCTTCC AGTTCGGCTT CTTTCCCTGG AATAAGGACT TGGAAATATA CAAGGAAAGG
    CCCTACTTTC CACCGAACAT CATCGGGGTG GAGAAGAAGG AGGGCTATGT TCTTTATGAC
    CTCATTCAGC TGCTGGCTCT ATTCTTCCAT CGCTCCATTC TGAAGTGTCA TGGTCTATGG
    GACGAGGATG ACATTGTTGA CAGTAACACA GACAAGGAAG GATCGGATGA CGAGCTGTCC
    CTCGATCAGG GCAGAAGAGG CTCCTCAGAC TCACTGAAGT CTATCAACCT GGCTGCCTCT
    GTAGAGTCTG TCCACGTGAC CTTCCCCGAG CAGCCAGCGG CCATCAGGAG AAAACGCTCC
    TGCAGTAGCT CTCAGATCTC CCCACGGTCC AGCTTCTCTT CAAATAGGTC CAAAAGAGGC
    AGCACAAGCA CCCGGAACAG TAGCCAGAAA GGAAGCAGTG TTTTAAGCCT TAAACAGAAA
    AGCAAACGAG AACTTTACAT GGAAAAGCTT CAAGAGCATT TAATCAAAGC AAAGGCGTTT
    ACCATAAAGA AGACTCTACA GATCTATGTC CCCATTCGGC AATTCTTCTA TGACCTCATC
    CACCCAGACT ACAGTGCTGT GACTGATGTA TACGTGCTGA TGTTTCTGGC TGACACTGTA
    GACTTCATTA TCATCGTCTT TGGATTCTGG GCCTTTGGGA AACACTCGGC AGCTGCAGAT
    ATCACCTCTT CTCTATCAGA AGACCAGGTC CCCGGGCCAT TCCTGGTGAT GGTCCTCATT
    CAGTTTGGAA CCATGGTGGT GGACCGTGCA CTCTACCTCA GGAAGACTGT GCTGGGCAAG
    GTCATCTTCC AGGTCATCCT CGTGTTTGGG ATTCACTTCT GGATGTTCTT CATCTTACCC
    GGTGTGACTG AGAGGAAATT CAGCCAGAAC CTGGTGGCAC AGCTTTGGTA CTTTGTGAAG
    TGTGTGTACT TTGGGCTGTC TGCCTATCAA ATCCGCTGTG GCTACCCAAC CCGTGTGCTC
    GGGAACTTCC TCACAAAGAG CTACAATTAC GTCAACCTCT TCTTGTTTCA AGGGTTCCGC
    CTGGTCCCAT TTCTGACTGA GCTGCGGGCT GTGATGGATT GGGTGTGGAC AGATACAACC
    CTGAGCCTCT CCAGCTGGAT TTGTGTGGAG GACATTTACG CGCACATTTT TATCTTAAAG
    TGCTGGCGAG AGTCGGAAAA GAGATACCCT CAACCCAGGG GTCAGAAGAA GAAGAAAGCC
    GTGAAGTACG GCATGGGCGG CATGATCATC GTCCTTCTCA TCTGTATCGT CTGGTTTCCT
    CTGCTCTTCA TGTCTTTGAT CAAATCTGTC GCAGGGGTTA TCAACCAACC CCTGGATGTG
    TCCGTCACAA TTACCCTGGG AGGTTATCAG CCTATCTTCA CAATGAGCGC CCAGCAAAGC
    CAGCTGAAAG TTATGGATAA TTCAAAGTAT AATGAATTTC TAAAATCTTT CGGCCCCAAC
    AGTGGCGCAA TGCAATTTCT GGAGAACTAT GAAAGGGAAG ACGTAACAGT AGCAGAACTG
    GAAGGGAACT CGAATTCTTT GTGGACCATC AGCCCTCCCA GTAAGCAGAA AATGATACAG
    GAGCTCACCG ACCCCAATAG TTGCTTCTCT GTTGTTTTTT CATGGAGTAT CCAGAGAAAC
    ATGACTTTGG GTGCAAAAGC CGAAATAGCA ACGGATAAGC TTTCTTTCCC TCTTGCGGTC
    GCAACACGGA ATAGCATAGC TAAAATGATT GCTGGCAATG ACACAGAAAG TTCAAACACG
    CCAGTGACAA TAGAGAAGAT CTACCCATAC TATGTGAAGG CACCCAGCGA TTCAAACTCA
    AAGCCTATAA AACAGCTTCT GTCTGAAAAT AATTTCATGA ATATCACCAT CATTTTGTTC
    AGAGACAATG TCACAAAGTC CAATAGTGAG TGGTGGGTTC TCAACCTGAC CGGAAGTAGG
    ATATTCAACC AGGGGTCCCA AGCCTTGGAA CTGGTGGTCT TCAATGACAA AGTCAGCCCT
    CCAAGTCTAG GCTTCTTGGC TGGCTATGGT ATCATGGGAT TGTATGCATC TGTTGTCCTT
    GTAATTGGGA AATTTGTTCG TGAGTTCTTC AGTGGGATCT CTCATTCCAT CATGTTTGAA
    GAGCTTCCAA ATGTGGACAG AATCTTGAAG TTGTGCACAG ATATATTCCT CGTGAGGGAG
    ACAGGGGAGT TGGAACTGGA GGAAGACCTC TACGCCAAGT TGATATTCCT GTACAGGTCA
    CCAGAAACCA TGATCAAGTG GACTAGAGAA AAAACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.