LOC100534973 cDNA ORF clone, Danio rerio(zebrafish)

The following LOC100534973 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100534973 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa150518 XM_021468270.1
Latest version!
Danio rerio piezo-type mechanosensitive ion channel component 2 (LOC100534973), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa150519 XM_021468271.1
Latest version!
Danio rerio piezo-type mechanosensitive ion channel component 2 (LOC100534973), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa150520 XM_017352447.2
Latest version!
Danio rerio piezo-type mechanosensitive ion channel component 2 (LOC100534973), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa150521 XM_021468277.1
Latest version!
Danio rerio piezo-type mechanosensitive ion channel component 2 (LOC100534973), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa56506 XM_017352447.1
Latest version!
Danio rerio piezo type mechanosensitive ion channel component 2 (piezo2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa150518
    Clone ID Related Accession (Same CDS sequence) XM_021468270.1
    Accession Version XM_021468270.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGCTTCTG AAGTTGTGTG TGGTTTGGTC TTCAGAGTGC TGTTGCCGGT GTGTCTGGCG 
    GCAGCATGTC TGTTCAGGTT CAATGGGCTG TCCTTCATCT ACCTCCTCTT CCTCCTCCTC
    ATCCCGCTGT TCCCAGAGCC CTCCAGCATC ACCATGAGAG GCCAGACGGG GTACTTGCTG
    AAGTCTTTAT GCTGCTGCAG TGTGACCTTC CTCTTCTTCC ACATCATCTA TCAAATCACC
    GTCCACAGTT TGCTGGCAAG AGGAAGAGTC GATTCTGGAT TCAACTGTTC GGTGTGGGAG
    AAGTCAATAC GACAGATTGG TTTTGAGAGT GTTATCGGGG CAGATGCAGG CAATGGTATC
    CGAGTTTTCC TGCCAGACAT TGGGATGTTC ATGGCCAGTC TGGGTGTGTG GCTCCTGTGC
    CGAAAACTGC TCCACCAACG GCCCACTGAG GATATGGCCC AGGACAACCA GCAATTCCTG
    TCTGAGGAGA AGGAGGAGGA AAACGAGAAG CTGGAAAATG AAGAAGATGA GAATGATAAT
    AATGATGATG ATGATGATGA AATGCTGTTT GACGAGGACT TTGATGCAGA AGATGAAGCA
    GAGGAAGAAG ATGGAGAAGC CGGAAGACTG GAGGAGGAGG AGGAGGAAGA GGAGGAGGAA
    GAGGAAGTGC AGGAGAGCAC CAAGATGAAG ATCCTGCGCG CGGTTGGAGG AGTCGCTGCC
    AAACTCAAGG AGATCATCGG GAACCTCATC ACTACTGCTG GACAGGTGGT GGTCACTATC
    ATGCTGGGAA TGACAGGTGT GACGCTGCCT TCTCTGTCCT CCGCTGTGTA TTTCTTCGTG
    TTTCTGGGTC TGTGCACATG GTGGTCTCTC TGTAAGACCT TCGATAAGCT CTTGTTCAGC
    TGTCTGTGTG TCCTGATGGC CATTTTCAGC GCTGGACATC TCATCGTGCT TTATATCAAC
    CAGTTTCAGT TCCTCCAGGA AGCCATAAGT CCCACCGATG GATATACCAG TGTTTTTGGC
    ATCTCCTCCA TCATTTACAC AGACTGCTCC TCTACATGGA AGCTTGCTGT CAGGAGTGGA
    CTCAAATGGC ATCATTTCGT CAATCCCATA ATGATCCTGG TCCTGTATTA CACACTGGCC
    ACGCTGATCC GCCTGTGGCT GCAGGATCCT ATGATGATGG AGGACAGCGA GGACGGAGAG
    GTGATCGAGG GCAATGAAGC CATGGAAAAC AACGTCCATC ACACGTCCAT CCGGAAACGG
    CTGCTCTGGT GGAAAGCGCA TCAGAAAACC GAGGAGAGAA ATCTGATCTC CACTCAGGAT
    GGGTACAGCA CCTCGGAGGT GTTAGTGGTC ACCTCCAATG GGACGTCGTT TGATTATGTG
    TCCGCCGTGC CCATCGAGAA CGGACCTGTC AGTCTGGACA TTTACTCCAC TCCTCAGTAC
    AAAGTGGATC AGTCTGTCGA ATATGACGAG AAGACGGATG AGAACACAAA TGAGGTGCTG
    GACTCACAGC TGGAGGAGGA AGAGGAGAAG ACAGATGAGG AAGAGGAAGC CGAAGAGGAA
    GAGCAGGGCG GACCACCGGC AGCTCTCGTC AAAGTGTTCA AGTTCATCAT GAAGCAGAGC
    TACATCTGCG CTCTCATCGC TATGATGGCG TGGAGTATAA CGTATGTCAG CTGGCTGACG
    TTTGTCTTTC TGATCTGGTC CTGTACTCTG TGGATGGTGC GCGACCGGAG GAAATACGCC
    ATGCTGACTT CGCCATTCAT GGTGGCTTAT GGGAACTTCC TGTTGGTCCT TCAGTATATC
    TGGTGTTTTG AGAAGTTGAA GCCGGTGTCT GGTTTCTTTG TGAAAATGGA GGTGCCTTTC
    ACTGAACTGA GCACCAAGGT TCTGTGTCAG CTGAGTTTCT GGCTACTGCT CAGACAAACT
    TTAATGGAGA GACAAGAAAG GAAGGAAGAA GAAGCAGCAC TTTCTGACAT CAAAGTAGAC
    GAGCAGAAAA AAGTGGAAGA AAAAAGTGAA GAGGAAGTGG ATGAGAACGA CCTGATGCAA
    GTGCTGGGCA AGCTGGTGAT GGCTCTGCTG GTCAAATACT GGATCTACGT CTGTGGAGGA
    ATGTTCTTCT TCGTCAGTTT TGAGGGAGAT ATGGCGACAT ATAAGATCAT CTACATGATG
    ATGCTGCTGT CCTGCGTCGC TCTTTACCAG GTACATTATG AATACTGGCG CAGGATCCTG
    AAGTATTTCT GGATGTCTGT GGTGGTTTAC ACTATGCTGG TGCTCATCCT GATCTACACC
    AGCCAGTTTG AGGATGGCAT CCCGACCTGG ACCAGGATGA CCGGCCTGGA CAAAGATGTA
    CTGAAGGATA TCGGTCTGGA GAAGTTCAGC CTCTCTAATT TGTTCACGCG TATCTTCATC
    CCGACGTCTT TCCTGTTGGT GTGTATCCTG CATCTGCACT ACTTCCATGA CCATTTTCTG
    GAGCTGACTG ATCTGAAAGC CGTGGTCAGC AAACAGGAGA GCACCATTTA CAGCTATGCT
    AAAGTCAGTG GTCGTATCTA TCTGATCGTG GATAGCATCA AGCAAAAAAT ATCTTCTTCT
    CAGAGCAAAT TGGCTCATCC CGATGGGAGT TTGGCGGATC TGACCATTGT GAGCTCGTCT
    CCGGAGCCGC TGCTGAAGGA TGAGAAGGAA AAAGAGTCTC TGAATGAGGT GCTGGTGGTG
    GATTGTGTGG GGGAAGAGAA GAAGAAAGAC TCTTTATCCA TTTATTCGGC TCAAATGTCC
    AGTCAGGAGG ACGTTCATCA GTCCAGTGCT GCCACAGACC CTGAACTGCC TTCAGAACAA
    AGCTCAGAGA TGAAGAATAA GTGGCATCTG GTGGTGGACC GGCTCACAGT GCTGTTCCTC
    AAGTTCCTGG AGTACTTTCA TAAGCTGAAA CTCTTTGTCT GGTGGCTGCT GGAGATGCAC
    ATCATCAAGA TCGTGGCCAC GTACATCATC CTGCTCTCGG TCAAAGAGGT CTCGCTGCTG
    AACTTTGTGT TTTTGATCTC CTGGGCGTTT GCGCTGCCGT ATAAGCAGTT TCGAGTTCTC
    TCCTCCAGCG TCTGCACCGT ATGGACGTGC GTTATTATTG TCTGCAAGCT CTTTTACCAG
    CTCAAAACCA TCAATCCTGC CAACTACTCC AGTGATTGTA TTATGCCCAA CTATACAGAA
    ACAGAAAAAA AGGACATGAA GACGTCTCTA CTGTATCGTG ATCCAGTGGA TCCTGCTAAC
    TGGGTTGGCC TGCGGAAGTT TGATCATGGG ATAATATTGC CTAACCTGAG GAATAACTTG
    CTGATGTTGG CCCTCTTGGC GTTCGAGGTT ACCATTTACA GACACCAGGA GTTTTTCCGT
    CTAAGAAACA AACTTTCTCC TCCTCCTTCA CGCACCATCT TTCATGACAT CACCCGGCAA
    CACCTTGACA ACGGCATCAT CGAGTGCGCC AAGTACTTTA TTAACTACTT CTTCTACAAG
    TTTGGTTTAG AGGTGTGTTT TTTGTTGGCC ATCAATGTGA TGGGTCAGCG GATGGATTTC
    TACTCCATGG TCCACGGCTT GGCTCTGGCT GTGGTGATGT TCAGACGGCG GAGGAAAGCC
    ATCGCTGAGA TCTGGCCCAA ATACTGCTGC TTCCTGGCCT GTATGATCAC CTTTCAGTAT
    TTCATATGCA TCGGGATTCC ACCGGCTGCT TGTGCAGATT ATCCCTGGAG GTTTCCCAAC
    TCCTCGATGA CGTCTAATGT CATTAAATGG CTTTACCTTC CTGATTTCCA CACTCCACCC
    AGCTCCATGT TCCTCGGCTA TGATTTCATG CTGCTGCTGT GTGCGTCGCT GCAGAGGCAG
    GTGTTTGATG AAGAAAATAA AGCCGCCGTT CGCCTGATGG CGGGAGATAA TGTGGAGATC
    TGCCGAGATC TGGACGCTGC ATCATTCAGT GTTCACAACC CGGTGCCAGA CTTCATTCAC
    TGCAGGTCAT ACCTGGACAT GCTGAAGGTG ATCATGTTCA GTTACCTGTT CTGGTTCGTC
    CTGACCATCA TCTTCATCAC GGGCACCACA CGGATCAGCA TCTTCTGCAT GGGTTACCTG
    GTGGCCTGCT TTTACTTCCT GCTGTTTGGT GGGCAACTTT TACTCAAGCC AATCAAAGAG
    ATCCTCCGTT ACTGGGACTT TCTCATCGCC TACAACGTTT TCGTCATCAC CATGAAGAAC
    GTCTTTGCAA TTTTGGCGTG CGGTTACATC AACTCGCTGG TGAAGAATAG CTGCTGGTTA
    ATTCAGCTGC TCAGTCTGGC CTGCACCATT AAAGACTACC AACCAAAAAC AGATCTGCCT
    GGAGTTGACT GTGAGCTGCC AAAAGACGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    ACCTTCCTCC TCCTGCAGAG AAGAGTCTTC ATGAGCTACT ATTTCTTGCA TGTAATCGCA
    GACATTCGAG CCGGACAGAT TCTTGCATCC CGAGGAGCTG AGTTGTTTCA GGCCAGTATC
    GTGAAGGCTG TGAGAGCTCG TCTGGAGGAG GAGAAGAAAT CCATGGAGCA ACTGAAGAGA
    CAGATGGATC GTATTAAAAC GAGACAGCAG AAGTTCAAGA GAGGAAAAGA GAAGATGCTC
    AGCATAGCCC AAGAGTCTGG AGACGGAGAG AAACTCATCC CACCAGTGGA CAATGAGGAG
    GAGGAGGACG AGGAGAAAAA GAAAAACAAG GTGCAAAAAA AGCAGTGGTG GAGGCCTTGG
    GTTGACCATG CGTCCATGGT TAGAAGTGGT GATTATTACC TGTTTGAAAC TGACAGTGAA
    GAGGAGGAGG AAGAGGAGGA GAAAAAAGAC GAAGAGCCTC CCAAAAGATC AGCCTTTCAG
    CGAGCGATCA GAAAGTTTGC TTCAGCCCTT GTTGCTTTAC CCAGATCAGT CATTAAACTG
    CCCAAAACTA TTCTGCAGTA TTTAATAAGG GCAGCCAAGT TTGTGTATCA TACCTGGATC
    GCCGAATCTA AAGCAGCTCT GAAAGAAAGA GGAAAAGGCA AGCGCTTCTG GAAGCGATAC
    GGTCGCCGGC TTAAAAAAGA GAAGAAAGAA GAACATGTGG CGATAGAGAT CGGTGAAGAC
    GAGCGTCAGA GTTCAGAAGA GGAGAAGACA GATGGACCAG ACAACATCAT CAAGAGGGTG
    TTCAACATCA TCAAGTTCAC CTGGGCTCTG TTTCTGACCA CCGTGGAGAG CATCACCAAA
    TGGCTGAACT CAGTCTGCAG AGAATATATC GACATCTCCA CCGTCCTGCG CATCGAGCGC
    TGCATGCTGA CCCGAGAAGT CAAAAAGGGG AATGTGCCGT CCCGTGAGAG CATCCATGTG
    TACTACCAGA AGCAGATGAA GCTGAATGGA TCTCGTGAAT CTGGTCTGGA CCGCATCAGT
    GAGGAGGATT CAGCCTCAAA CAGAGAACGC CGCAGACGCG CACAACACAG TCTGGATTCA
    TTCGCTTCCA GAGACAGCAT CTCCAGCGCA TACACCGAAG CCACCATGCT GTTTTCCCGC
    CAGTCCACCT TGGACGATTT GGACGACATG CCTCAGAATA TTCCCAAAAC CAGCGAACGC
    GCTCGACCCA AACTGCGCAA AATGTACGGC CTCGACATGT CCAACTCCTC TGCGGACAGC
    GGAAGCAGCG TCATCTCCAG TGAGGCTACT CAGTGCGTCA CGCTGTTTTC TCGGCAAGGA
    ACCAACGACA CTATCGACGA GGCTGAAGAC GAGCGAGAAC AGCTGGAGCA CAAGCAGAAA
    ACAGAAGAAG CAGAACTCAG AGACAAACCT GAAGAAGAGC AGGAAGTGGA AATAAGCGAT
    CAGGGGAAAC AGGAAGTGTT ACCGGTGAGC CATCTGGTGA CACAGGAAGT GAAAGAGGAT
    GTACTACCTG TGAGCGATCA GCTGACTGGG GAAGTGACTG AGGAAGTTGA AGAGGAGGTG
    GAAGTAGTTA AGGAAATGGA AGAGGAAGTA GAAAAGGAAA TTGGAGCAAA GGTGGAAAAG
    GAAGTAGAAG CGGAAAAGGA AGAGGAAGCG GAAAAGGAAG TGGGTGAGGA AGTGCAATGG
    GACTTGGTGG ATCCAGAGGA AGCAGAGGAG TGTGTAAGTG ATGGGGACGG TGGTGAGCAG
    ATGCAGCAGG ATGAAGGTGA ATGGTCGGGA GTGGAGAACG TGGTGAGCGG TCCGCTGTTC
    ACACCAGACA CTGACGCCCC CAACACGTCT GACGCTGACG TGCCGCCCAG CTACAGTAAA
    GCCGTGAGCT TCGACCGGCT GTCTGTGAGC TCGGACGACA GTGACAGCGA CAAACGGCTG
    ATGCTAATGA CGCCTGACAG CAAATCTGAC CTGGACGACC CTCTGCTGCC CTCCATGACC
    ACCGACATGA CCGCCAGCGA ACTGCTGCTC AACAAAATGT TCTACGACGA GATGCTGGAC
    AGCTCAGATC GCTTCTATAA GTCTCAGCCG CTGGGTCTCC AGTTGTGTTA CGCTCTCTAC
    AACCTGCTGG TGGCTCACTC AGAAATGGTG TGTTATCTGG TCATCATCCT CAACCACATG
    ATCTCCGCCT CCATGGCCAC GCTGGTGCTG CCCATCCTCA TCTTCCTGTG GGCCATGCTG
    TCCGTTCCGC GACCCAGCAA GAGGTTCTGG ATGACCGCCA TCGTCTACAC TGAGGTCACC
    ATCGTCATCA AATACTTCTT CCAGTTCAGC TTTTTCCCTT TCAATCAGAA CCTGGAGGTG
    AACAAGGGCA AGCCGTACCA CCCGCCCAAC ATCCTGGGCA CAGAGAAAAA AGACGGATAC
    GTGCACTACG ACCTGGTCCA GCTGCTGGCG CTCTTCTTCC ACAGATCCAT ACTAAAGTGT
    CATGGTCTCT GGGACGAGGA CGACCCCAAA GTGCCCCGTA AGGATGAGTG TCCTCGTCAG
    GAGTCTGTGG ATGACGGGAA GCTGTCGGTG GTCATGTCAC CTGATGAAGA CAGGAAGAGT
    TCTCCGGCGT ATTCACTCAA GTCTATGAAT CTGGGAATGT CTGTGGACTC ATCGCAGGTT
    CACGTACAGA TGCGTTATCC AGAGCAGCGG CCGTACCTGC GCAGACAGAG CACCAGCGGA
    TCACACTTCT CCCACAGATC CTCCGCACGC TCCAAACGCG GCAGCACAAG CACACGTCAC
    AGCACCCATG GGGAAAACAG CACAACAGAG TCAGAAGTAC CACAAAAGAG CCGGAAAGAA
    ATGATTATGG AGAAAATCAG AGAGCAGCTC ATCAAAGCAA AGGTTTACCT GCTCAAAAGA
    TTGGTTGAGT TTTATCAGCC CATCCGTCAG TTCTTCTACA ACCTGGTTCA TCCGGAGTAC
    AGCGCCGTGA CGGATGTTTA CGTATTGATG TTTCTAGCAG ACACTGTAGA TTTTATCATC
    ATCGTCTTTG GCTTTTGGGC ATTTGGGAAA CATCAGGGTG GTGCTGACAT CACTTCCTCC
    CTGTCAGAGG ACCAGGTTCC TGGGCCGTTC CTTGTTATGG TTCTCATACA GTTTGGGACG
    ATGGTAGTGG ATCGAGCCCT GTATCTGCGC AAGACTGTTG TGGGAAAAGT GATTTTTCAG
    GTCATCCTGG TGTTTGGCAT CCATTTCTGG ATGTTTTTCA TCCTGCCGGG AATCACAGAG
    AGGCGCTTCA GTCAGAACAC TATCGCTCAG CTCTGGTACT TTGTGAAGTG CATTTACTTC
    GGCCTGTCAG CCTATCAGAT CAGATGTGGA TATCCAACCC GCATCCTGGG AAACTTCCTC
    ACCAAGAGCT ACAACTACGC CAACCTCTTC CTCTTCCAGG GTTTCCGTCT GATCCCGTTC
    CTGACGGAGC TGCGTGCGGT GATGGACTGG GTCTGGACGG ACACAACGCT CAGCTTGTCC
    AGCTGGATCT GTGTGGAGGA CATCTATGCT CACATCTTCA TCCTCAAATG CTGGAGGGAG
    TCTGAGAAGC GTTACCCTCA GCCGCGCGGG CAGAAGAAGA AGAAGGTGGT GAAGTATGGG
    ATGGGCGGCA TGATCGTGAT GCTCCTGATC TGCATCGTCT GGTTTCCTCT GCTCTTCATG
    TCTCTGGTCA AATCTGTGGC AGGAGTGGTT AACAAGCCTC TGGAGGTGTC CGTCTCGCTC
    ACGCTCGGAG GACTCCAGCC GATCTTCACA ATGAGTGCCC AGCAGAACCA TATCAAATCC
    GTCACTGGGG CAGAATTTAA GAAGTTCACA GATCACTACA AGTCTAACGC AAATGCCATC
    ATGTTCCTGG AGTCGTATTT TCCTGAGGAT CTGACAATAG CACATCTAGA GGGAAGTTCA
    AACTCACTCT GGACCATCAG CCCCCCGAGC AAGGAAAACC TGAAGAATAT GCTCAATGAC
    ACGAGAGAGC CGTTTCCTCT CACCTTCACC TGGTCCATTC AAAGGAACCT CAGTTTAGGT
    GCCAAAGCTG AAACTGCAAT GGGGAAATAC TTCATTGAGC TGGACGACGA GACTAGAAAA
    GGTCTAACTG ATCAACTGGG GAATAGCAGC CAGCGTGTGA CAATCAAAAA AATATTTCCA
    AGGTACATCA GAGCACCAAG CGACTCCAAT GCCAAACCTA TTGACCAGCT TTACAACGAT
    GGAGAGTACA TGGACATCAC ACTGTTTTTA AATAAGAAGA ACAGCTCAGA AGGCGTTCAG
    GAGTGGTGGA TCGTCAATCA GACAGATTCA GGTCCATTAC CTGGACTCAA CGGGTCTGGT
    CTTCAGATCT ACATCATCAG TGACCAAGTC AGTCCACCCA GTCTGGGCTT TCTGGCTGGT
    TATGGGATCA TGGGTCTGTA CATGTCAGTG GTTTTGGTGA TCGGGAAGTT TGTGCGCGAG
    TTCTTCAGCG GGATCTCGCA CACCATCATG TTCGAAGAGC TCCCTAACGT GGACCGAATC
    CTCAAACTCT GCACCGATAT CTTCCTGGTC CGAGAGACGG GCGAACTGGA CCTGGAGGAA
    GACCTTTACG CCAAACTCAT CTTCCTCTAC CGCTCTCCAG AAACCATGAT CAAATGGACC
    AGAGAGAAGA GTGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323945.1
    CDS675..9692
    Translation

    Target ORF information:

    RefSeq Version XM_021468270.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio piezo-type mechanosensitive ion channel component 2 (LOC100534973), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468270.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGCTTCTG AAGTTGTGTG TGGTTTGGTC TTCAGAGTGC TGTTGCCGGT GTGTCTGGCG 
    GCAGCATGTC TGTTCAGGTT CAATGGGCTG TCCTTCATCT ACCTCCTCTT CCTCCTCCTC
    ATCCCGCTGT TCCCAGAGCC CTCCAGCATC ACCATGAGAG GCCAGACGGG GTACTTGCTG
    AAGTCTTTAT GCTGCTGCAG TGTGACCTTC CTCTTCTTCC ACATCATCTA TCAAATCACC
    GTCCACAGTT TGCTGGCAAG AGGAAGAGTC GATTCTGGAT TCAACTGTTC GGTGTGGGAG
    AAGTCAATAC GACAGATTGG TTTTGAGAGT GTTATCGGGG CAGATGCAGG CAATGGTATC
    CGAGTTTTCC TGCCAGACAT TGGGATGTTC ATGGCCAGTC TGGGTGTGTG GCTCCTGTGC
    CGAAAACTGC TCCACCAACG GCCCACTGAG GATATGGCCC AGGACAACCA GCAATTCCTG
    TCTGAGGAGA AGGAGGAGGA AAACGAGAAG CTGGAAAATG AAGAAGATGA GAATGATAAT
    AATGATGATG ATGATGATGA AATGCTGTTT GACGAGGACT TTGATGCAGA AGATGAAGCA
    GAGGAAGAAG ATGGAGAAGC CGGAAGACTG GAGGAGGAGG AGGAGGAAGA GGAGGAGGAA
    GAGGAAGTGC AGGAGAGCAC CAAGATGAAG ATCCTGCGCG CGGTTGGAGG AGTCGCTGCC
    AAACTCAAGG AGATCATCGG GAACCTCATC ACTACTGCTG GACAGGTGGT GGTCACTATC
    ATGCTGGGAA TGACAGGTGT GACGCTGCCT TCTCTGTCCT CCGCTGTGTA TTTCTTCGTG
    TTTCTGGGTC TGTGCACATG GTGGTCTCTC TGTAAGACCT TCGATAAGCT CTTGTTCAGC
    TGTCTGTGTG TCCTGATGGC CATTTTCAGC GCTGGACATC TCATCGTGCT TTATATCAAC
    CAGTTTCAGT TCCTCCAGGA AGCCATAAGT CCCACCGATG GATATACCAG TGTTTTTGGC
    ATCTCCTCCA TCATTTACAC AGACTGCTCC TCTACATGGA AGCTTGCTGT CAGGAGTGGA
    CTCAAATGGC ATCATTTCGT CAATCCCATA ATGATCCTGG TCCTGTATTA CACACTGGCC
    ACGCTGATCC GCCTGTGGCT GCAGGATCCT ATGATGATGG AGGACAGCGA GGACGGAGAG
    GTGATCGAGG GCAATGAAGC CATGGAAAAC AACGTCCATC ACACGTCCAT CCGGAAACGG
    CTGCTCTGGT GGAAAGCGCA TCAGAAAACC GAGGAGAGAA ATCTGATCTC CACTCAGGAT
    GGGTACAGCA CCTCGGAGGT GTTAGTGGTC ACCTCCAATG GGACGTCGTT TGATTATGTG
    TCCGCCGTGC CCATCGAGAA CGGACCTGTC AGTCTGGACA TTTACTCCAC TCCTCAGTAC
    AAAGTGGATC AGTCTGTCGA ATATGACGAG AAGACGGATG AGAACACAAA TGAGGTGCTG
    GACTCACAGC TGGAGGAGGA AGAGGAGAAG ACAGATGAGG AAGAGGAAGC CGAAGAGGAA
    GAGCAGGGCG GACCACCGGC AGCTCTCGTC AAAGTGTTCA AGTTCATCAT GAAGCAGAGC
    TACATCTGCG CTCTCATCGC TATGATGGCG TGGAGTATAA CGTATGTCAG CTGGCTGACG
    TTTGTCTTTC TGATCTGGTC CTGTACTCTG TGGATGGTGC GCGACCGGAG GAAATACGCC
    ATGCTGACTT CGCCATTCAT GGTGGCTTAT GGGAACTTCC TGTTGGTCCT TCAGTATATC
    TGGTGTTTTG AGAAGTTGAA GCCGGTGTCT GGTTTCTTTG TGAAAATGGA GGTGCCTTTC
    ACTGAACTGA GCACCAAGGT TCTGTGTCAG CTGAGTTTCT GGCTACTGCT CAGACAAACT
    TTAATGGAGA GACAAGAAAG GAAGGAAGAA GAAGCAGCAC TTTCTGACAT CAAAGTAGAC
    GAGCAGAAAA AAGTGGAAGA AAAAAGTGAA GAGGAAGTGG ATGAGAACGA CCTGATGCAA
    GTGCTGGGCA AGCTGGTGAT GGCTCTGCTG GTCAAATACT GGATCTACGT CTGTGGAGGA
    ATGTTCTTCT TCGTCAGTTT TGAGGGAGAT ATGGCGACAT ATAAGATCAT CTACATGATG
    ATGCTGCTGT CCTGCGTCGC TCTTTACCAG GTACATTATG AATACTGGCG CAGGATCCTG
    AAGTATTTCT GGATGTCTGT GGTGGTTTAC ACTATGCTGG TGCTCATCCT GATCTACACC
    AGCCAGTTTG AGGATGGCAT CCCGACCTGG ACCAGGATGA CCGGCCTGGA CAAAGATGTA
    CTGAAGGATA TCGGTCTGGA GAAGTTCAGC CTCTCTAATT TGTTCACGCG TATCTTCATC
    CCGACGTCTT TCCTGTTGGT GTGTATCCTG CATCTGCACT ACTTCCATGA CCATTTTCTG
    GAGCTGACTG ATCTGAAAGC CGTGGTCAGC AAACAGGAGA GCACCATTTA CAGCTATGCT
    AAAGTCAGTG GTCGTATCTA TCTGATCGTG GATAGCATCA AGCAAAAAAT ATCTTCTTCT
    CAGAGCAAAT TGGCTCATCC CGATGGGAGT TTGGCGGATC TGACCATTGT GAGCTCGTCT
    CCGGAGCCGC TGCTGAAGGA TGAGAAGGAA AAAGAGTCTC TGAATGAGGT GCTGGTGGTG
    GATTGTGTGG GGGAAGAGAA GAAGAAAGAC TCTTTATCCA TTTATTCGGC TCAAATGTCC
    AGTCAGGAGG ACGTTCATCA GTCCAGTGCT GCCACAGACC CTGAACTGCC TTCAGAACAA
    AGCTCAGAGA TGAAGAATAA GTGGCATCTG GTGGTGGACC GGCTCACAGT GCTGTTCCTC
    AAGTTCCTGG AGTACTTTCA TAAGCTGAAA CTCTTTGTCT GGTGGCTGCT GGAGATGCAC
    ATCATCAAGA TCGTGGCCAC GTACATCATC CTGCTCTCGG TCAAAGAGGT CTCGCTGCTG
    AACTTTGTGT TTTTGATCTC CTGGGCGTTT GCGCTGCCGT ATAAGCAGTT TCGAGTTCTC
    TCCTCCAGCG TCTGCACCGT ATGGACGTGC GTTATTATTG TCTGCAAGCT CTTTTACCAG
    CTCAAAACCA TCAATCCTGC CAACTACTCC AGTGATTGTA TTATGCCCAA CTATACAGAA
    ACAGAAAAAA AGGACATGAA GACGTCTCTA CTGTATCGTG ATCCAGTGGA TCCTGCTAAC
    TGGGTTGGCC TGCGGAAGTT TGATCATGGG ATAATATTGC CTAACCTGAG GAATAACTTG
    CTGATGTTGG CCCTCTTGGC GTTCGAGGTT ACCATTTACA GACACCAGGA GTTTTTCCGT
    CTAAGAAACA AACTTTCTCC TCCTCCTTCA CGCACCATCT TTCATGACAT CACCCGGCAA
    CACCTTGACA ACGGCATCAT CGAGTGCGCC AAGTACTTTA TTAACTACTT CTTCTACAAG
    TTTGGTTTAG AGGTGTGTTT TTTGTTGGCC ATCAATGTGA TGGGTCAGCG GATGGATTTC
    TACTCCATGG TCCACGGCTT GGCTCTGGCT GTGGTGATGT TCAGACGGCG GAGGAAAGCC
    ATCGCTGAGA TCTGGCCCAA ATACTGCTGC TTCCTGGCCT GTATGATCAC CTTTCAGTAT
    TTCATATGCA TCGGGATTCC ACCGGCTGCT TGTGCAGATT ATCCCTGGAG GTTTCCCAAC
    TCCTCGATGA CGTCTAATGT CATTAAATGG CTTTACCTTC CTGATTTCCA CACTCCACCC
    AGCTCCATGT TCCTCGGCTA TGATTTCATG CTGCTGCTGT GTGCGTCGCT GCAGAGGCAG
    GTGTTTGATG AAGAAAATAA AGCCGCCGTT CGCCTGATGG CGGGAGATAA TGTGGAGATC
    TGCCGAGATC TGGACGCTGC ATCATTCAGT GTTCACAACC CGGTGCCAGA CTTCATTCAC
    TGCAGGTCAT ACCTGGACAT GCTGAAGGTG ATCATGTTCA GTTACCTGTT CTGGTTCGTC
    CTGACCATCA TCTTCATCAC GGGCACCACA CGGATCAGCA TCTTCTGCAT GGGTTACCTG
    GTGGCCTGCT TTTACTTCCT GCTGTTTGGT GGGCAACTTT TACTCAAGCC AATCAAAGAG
    ATCCTCCGTT ACTGGGACTT TCTCATCGCC TACAACGTTT TCGTCATCAC CATGAAGAAC
    GTCTTTGCAA TTTTGGCGTG CGGTTACATC AACTCGCTGG TGAAGAATAG CTGCTGGTTA
    ATTCAGCTGC TCAGTCTGGC CTGCACCATT AAAGACTACC AACCAAAAAC AGATCTGCCT
    GGAGTTGACT GTGAGCTGCC AAAAGACGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    ACCTTCCTCC TCCTGCAGAG AAGAGTCTTC ATGAGCTACT ATTTCTTGCA TGTAATCGCA
    GACATTCGAG CCGGACAGAT TCTTGCATCC CGAGGAGCTG AGTTGTTTCA GGCCAGTATC
    GTGAAGGCTG TGAGAGCTCG TCTGGAGGAG GAGAAGAAAT CCATGGAGCA ACTGAAGAGA
    CAGATGGATC GTATTAAAAC GAGACAGCAG AAGTTCAAGA GAGGAAAAGA GAAGATGCTC
    AGCATAGCCC AAGAGTCTGG AGACGGAGAG AAACTCATCC CACCAGTGGA CAATGAGGAG
    GAGGAGGACG AGGAGAAAAA GAAAAACAAG GTGCAAAAAA AGCAGTGGTG GAGGCCTTGG
    GTTGACCATG CGTCCATGGT TAGAAGTGGT GATTATTACC TGTTTGAAAC TGACAGTGAA
    GAGGAGGAGG AAGAGGAGGA GAAAAAAGAC GAAGAGCCTC CCAAAAGATC AGCCTTTCAG
    CGAGCGATCA GAAAGTTTGC TTCAGCCCTT GTTGCTTTAC CCAGATCAGT CATTAAACTG
    CCCAAAACTA TTCTGCAGTA TTTAATAAGG GCAGCCAAGT TTGTGTATCA TACCTGGATC
    GCCGAATCTA AAGCAGCTCT GAAAGAAAGA GGAAAAGGCA AGCGCTTCTG GAAGCGATAC
    GGTCGCCGGC TTAAAAAAGA GAAGAAAGAA GAACATGTGG CGATAGAGAT CGGTGAAGAC
    GAGCGTCAGA GTTCAGAAGA GGAGAAGACA GATGGACCAG ACAACATCAT CAAGAGGGTG
    TTCAACATCA TCAAGTTCAC CTGGGCTCTG TTTCTGACCA CCGTGGAGAG CATCACCAAA
    TGGCTGAACT CAGTCTGCAG AGAATATATC GACATCTCCA CCGTCCTGCG CATCGAGCGC
    TGCATGCTGA CCCGAGAAGT CAAAAAGGGG AATGTGCCGT CCCGTGAGAG CATCCATGTG
    TACTACCAGA AGCAGATGAA GCTGAATGGA TCTCGTGAAT CTGGTCTGGA CCGCATCAGT
    GAGGAGGATT CAGCCTCAAA CAGAGAACGC CGCAGACGCG CACAACACAG TCTGGATTCA
    TTCGCTTCCA GAGACAGCAT CTCCAGCGCA TACACCGAAG CCACCATGCT GTTTTCCCGC
    CAGTCCACCT TGGACGATTT GGACGACATG CCTCAGAATA TTCCCAAAAC CAGCGAACGC
    GCTCGACCCA AACTGCGCAA AATGTACGGC CTCGACATGT CCAACTCCTC TGCGGACAGC
    GGAAGCAGCG TCATCTCCAG TGAGGCTACT CAGTGCGTCA CGCTGTTTTC TCGGCAAGGA
    ACCAACGACA CTATCGACGA GGCTGAAGAC GAGCGAGAAC AGCTGGAGCA CAAGCAGAAA
    ACAGAAGAAG CAGAACTCAG AGACAAACCT GAAGAAGAGC AGGAAGTGGA AATAAGCGAT
    CAGGGGAAAC AGGAAGTGTT ACCGGTGAGC CATCTGGTGA CACAGGAAGT GAAAGAGGAT
    GTACTACCTG TGAGCGATCA GCTGACTGGG GAAGTGACTG AGGAAGTTGA AGAGGAGGTG
    GAAGTAGTTA AGGAAATGGA AGAGGAAGTA GAAAAGGAAA TTGGAGCAAA GGTGGAAAAG
    GAAGTAGAAG CGGAAAAGGA AGAGGAAGCG GAAAAGGAAG TGGGTGAGGA AGTGCAATGG
    GACTTGGTGG ATCCAGAGGA AGCAGAGGAG TGTGTAAGTG ATGGGGACGG TGGTGAGCAG
    ATGCAGCAGG ATGAAGGTGA ATGGTCGGGA GTGGAGAACG TGGTGAGCGG TCCGCTGTTC
    ACACCAGACA CTGACGCCCC CAACACGTCT GACGCTGACG TGCCGCCCAG CTACAGTAAA
    GCCGTGAGCT TCGACCGGCT GTCTGTGAGC TCGGACGACA GTGACAGCGA CAAACGGCTG
    ATGCTAATGA CGCCTGACAG CAAATCTGAC CTGGACGACC CTCTGCTGCC CTCCATGACC
    ACCGACATGA CCGCCAGCGA ACTGCTGCTC AACAAAATGT TCTACGACGA GATGCTGGAC
    AGCTCAGATC GCTTCTATAA GTCTCAGCCG CTGGGTCTCC AGTTGTGTTA CGCTCTCTAC
    AACCTGCTGG TGGCTCACTC AGAAATGGTG TGTTATCTGG TCATCATCCT CAACCACATG
    ATCTCCGCCT CCATGGCCAC GCTGGTGCTG CCCATCCTCA TCTTCCTGTG GGCCATGCTG
    TCCGTTCCGC GACCCAGCAA GAGGTTCTGG ATGACCGCCA TCGTCTACAC TGAGGTCACC
    ATCGTCATCA AATACTTCTT CCAGTTCAGC TTTTTCCCTT TCAATCAGAA CCTGGAGGTG
    AACAAGGGCA AGCCGTACCA CCCGCCCAAC ATCCTGGGCA CAGAGAAAAA AGACGGATAC
    GTGCACTACG ACCTGGTCCA GCTGCTGGCG CTCTTCTTCC ACAGATCCAT ACTAAAGTGT
    CATGGTCTCT GGGACGAGGA CGACCCCAAA GTGCCCCGTA AGGATGAGTG TCCTCGTCAG
    GAGTCTGTGG ATGACGGGAA GCTGTCGGTG GTCATGTCAC CTGATGAAGA CAGGAAGAGT
    TCTCCGGCGT ATTCACTCAA GTCTATGAAT CTGGGAATGT CTGTGGACTC ATCGCAGGTT
    CACGTACAGA TGCGTTATCC AGAGCAGCGG CCGTACCTGC GCAGACAGAG CACCAGCGGA
    TCACACTTCT CCCACAGATC CTCCGCACGC TCCAAACGCG GCAGCACAAG CACACGTCAC
    AGCACCCATG GGGAAAACAG CACAACAGAG TCAGAAGTAC CACAAAAGAG CCGGAAAGAA
    ATGATTATGG AGAAAATCAG AGAGCAGCTC ATCAAAGCAA AGGTTTACCT GCTCAAAAGA
    TTGGTTGAGT TTTATCAGCC CATCCGTCAG TTCTTCTACA ACCTGGTTCA TCCGGAGTAC
    AGCGCCGTGA CGGATGTTTA CGTATTGATG TTTCTAGCAG ACACTGTAGA TTTTATCATC
    ATCGTCTTTG GCTTTTGGGC ATTTGGGAAA CATCAGGGTG GTGCTGACAT CACTTCCTCC
    CTGTCAGAGG ACCAGGTTCC TGGGCCGTTC CTTGTTATGG TTCTCATACA GTTTGGGACG
    ATGGTAGTGG ATCGAGCCCT GTATCTGCGC AAGACTGTTG TGGGAAAAGT GATTTTTCAG
    GTCATCCTGG TGTTTGGCAT CCATTTCTGG ATGTTTTTCA TCCTGCCGGG AATCACAGAG
    AGGCGCTTCA GTCAGAACAC TATCGCTCAG CTCTGGTACT TTGTGAAGTG CATTTACTTC
    GGCCTGTCAG CCTATCAGAT CAGATGTGGA TATCCAACCC GCATCCTGGG AAACTTCCTC
    ACCAAGAGCT ACAACTACGC CAACCTCTTC CTCTTCCAGG GTTTCCGTCT GATCCCGTTC
    CTGACGGAGC TGCGTGCGGT GATGGACTGG GTCTGGACGG ACACAACGCT CAGCTTGTCC
    AGCTGGATCT GTGTGGAGGA CATCTATGCT CACATCTTCA TCCTCAAATG CTGGAGGGAG
    TCTGAGAAGC GTTACCCTCA GCCGCGCGGG CAGAAGAAGA AGAAGGTGGT GAAGTATGGG
    ATGGGCGGCA TGATCGTGAT GCTCCTGATC TGCATCGTCT GGTTTCCTCT GCTCTTCATG
    TCTCTGGTCA AATCTGTGGC AGGAGTGGTT AACAAGCCTC TGGAGGTGTC CGTCTCGCTC
    ACGCTCGGAG GACTCCAGCC GATCTTCACA ATGAGTGCCC AGCAGAACCA TATCAAATCC
    GTCACTGGGG CAGAATTTAA GAAGTTCACA GATCACTACA AGTCTAACGC AAATGCCATC
    ATGTTCCTGG AGTCGTATTT TCCTGAGGAT CTGACAATAG CACATCTAGA GGGAAGTTCA
    AACTCACTCT GGACCATCAG CCCCCCGAGC AAGGAAAACC TGAAGAATAT GCTCAATGAC
    ACGAGAGAGC CGTTTCCTCT CACCTTCACC TGGTCCATTC AAAGGAACCT CAGTTTAGGT
    GCCAAAGCTG AAACTGCAAT GGGGAAATAC TTCATTGAGC TGGACGACGA GACTAGAAAA
    GGTCTAACTG ATCAACTGGG GAATAGCAGC CAGCGTGTGA CAATCAAAAA AATATTTCCA
    AGGTACATCA GAGCACCAAG CGACTCCAAT GCCAAACCTA TTGACCAGCT TTACAACGAT
    GGAGAGTACA TGGACATCAC ACTGTTTTTA AATAAGAAGA ACAGCTCAGA AGGCGTTCAG
    GAGTGGTGGA TCGTCAATCA GACAGATTCA GGTCCATTAC CTGGACTCAA CGGGTCTGGT
    CTTCAGATCT ACATCATCAG TGACCAAGTC AGTCCACCCA GTCTGGGCTT TCTGGCTGGT
    TATGGGATCA TGGGTCTGTA CATGTCAGTG GTTTTGGTGA TCGGGAAGTT TGTGCGCGAG
    TTCTTCAGCG GGATCTCGCA CACCATCATG TTCGAAGAGC TCCCTAACGT GGACCGAATC
    CTCAAACTCT GCACCGATAT CTTCCTGGTC CGAGAGACGG GCGAACTGGA CCTGGAGGAA
    GACCTTTACG CCAAACTCAT CTTCCTCTAC CGCTCTCCAG AAACCATGAT CAAATGGACC
    AGAGAGAAGA GTGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150519
    Clone ID Related Accession (Same CDS sequence) XM_021468271.1
    Accession Version XM_021468271.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
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    3721
    3781
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    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCTTCTG AAGTTGTGTG TGGTTTGGTC TTCAGAGTGC TGTTGCCGGT GTGTCTGGCG 
    GCAGCATGTC TGTTCAGGTT CAATGGGCTG TCCTTCATCT ACCTCCTCTT CCTCCTCCTC
    ATCCCGCTGT TCCCAGAGCC CTCCAGCATC ACCATGAGAG GCCAGACGGG GTACTTGCTG
    AAGTCTTTAT GCTGCTGCAG TGTGACCTTC CTCTTCTTCC ACATCATCTA TCAAATCACC
    GTCCACAGTT TGCTGGCAAG AGGAAGAGTC GATTCTGGAT TCAACTGTTC GGTGTGGGAG
    AAGTCAATAC GACAGATTGG TTTTGAGAGT GTTATCGGGG CAGATGCAGG CAATGGTATC
    CGAGTTTTCC TGCCAGACAT TGGGATGTTC ATGGCCAGTC TGGGTGTGTG GCTCCTGTGC
    CGAAAACTGC TCCACCAACG GCCCACTGAG GATATGGCCC AGGACAACCA GCAATTCCTG
    TCTGAGGAGA AGGAGGAGGA AAACGAGAAG CTGGAAAATG AAGAAGATGA GAATGATAAT
    AATGATGATG ATGATGATGA AATGCTGTTT GACGAGGACT TTGATGCAGA AGATGAAGCA
    GAGGAAGAAG ATGGAGAAGC CGGAAGACTG GAGGAGGAGG AGGAGGAAGA GGAGGAGGAA
    GAGGAAGTGC AGGAGAGCAC CAAGATGAAG ATCCTGCGCG CGGTTGGAGG AGTCGCTGCC
    AAACTCAAGG AGATCATCGG GAACCTCATC ACTACTGCTG GACAGGTGGT GGTCACTATC
    ATGCTGGGAA TGACAGGTGT GACGCTGCCT TCTCTGTCCT CCGCTGTGTA TTTCTTCGTG
    TTTCTGGGTC TGTGCACATG GTGGTCTCTC TGTAAGACCT TCGATAAGCT CTTGTTCAGC
    TGTCTGTGTG TCCTGATGGC CATTTTCAGC GCTGGACATC TCATCGTGCT TTATATCAAC
    CAGTTTCAGT TCCTCCAGGA AGCCATAAGT CCCACCGATG GATATACCAG TGTTTTTGGC
    ATCTCCTCCA TCATTTACAC AGACTGCTCC TCTACATGGA AGCTTGCTGT CAGGAGTGGA
    CTCAAATGGC ATCATTTCGT CAATCCCATA ATGATCCTGG TCCTGTATTA CACACTGGCC
    ACGCTGATCC GCCTGTGGCT GCAGGATCCT ATGATGATGG AGGACAGCGA GGACGGAGAG
    GTGATCGAGG GCAATGAAGC CATGGAAAAC AACGTCCATC ACACGTCCAT CCGGAAACGG
    CTGCTCTGGT GGAAAGCGCA TCAGAAAACC GAGGAGAGAA ATCTGATCTC CACTCAGGAT
    GGGTACAGCA CCTCGGAGGT GTTAGTGGTC ACCTCCAATG GGACGTCGTT TGATTATGTG
    TCCGCCGTGC CCATCGAGAA CGGACCTGTC AGTCTGGACA TTTACTCCAC TCCTCAGTAC
    AAAGTGGATC AGTCTGTCGA ATATGACGAG AAGACGGATG AGAACACAAA TGAGGTGCTG
    GACTCACAGC TGGAGGAGGA AGAGGAGAAG ACAGATGAGG AAGAGGAAGC CGAAGAGGAA
    GAGCAGGGCG GACCACCGGC AGCTCTCGTC AAAGTGTTCA AGTTCATCAT GAAGCAGAGC
    TACATCTGCG CTCTCATCGC TATGATGGCG TGGAGTATAA CGTATGTCAG CTGGCTGACG
    TTTGTCTTTC TGATCTGGTC CTGTACTCTG TGGATGGTGC GCGACCGGAG GAAATACGCC
    ATGCTGACTT CGCCATTCAT GGTGGCTTAT GGGAACTTCC TGTTGGTCCT TCAGTATATC
    TGGTGTTTTG AGAAGTTGAA GCCGGTGTCT GGTTTCTTTG TGAAAATGGA GGTGCCTTTC
    ACTGAACTGA GCACCAAGGT TCTGTGTCAG CTGAGTTTCT GGCTACTGCT CAGACAAACT
    TTAATGGAGA GACAAGAAAG GAAGGAAGAA GAAGCAGCAC TTTCTGACAT CAAAGTAGAC
    GAGCAGAAAA AAGTGGAAGA AAAAAGTGAA GAGGAAGTGG ATGAGAACGA CCTGATGCAA
    GTGCTGGGCA AGCTGGTGAT GGCTCTGCTG GTCAAATACT GGATCTACGT CTGTGGAGGA
    ATGTTCTTCT TCGTCAGTTT TGAGGGAGAT ATGGCGACAT ATAAGATCAT CTACATGATG
    ATGCTGCTGT CCTGCGTCGC TCTTTACCAG GTACATTATG AATACTGGCG CAGGATCCTG
    AAGTATTTCT GGATGTCTGT GGTGGTTTAC ACTATGCTGG TGCTCATCCT GATCTACACC
    AGCCAGTTTG AGGATGGCAT CCCGACCTGG ACCAGGATGA CCGGCCTGGA CAAAGATGTA
    CTGAAGGATA TCGGTCTGGA GAAGTTCAGC CTCTCTAATT TGTTCACGCG TATCTTCATC
    CCGACGTCTT TCCTGTTGGT GTGTATCCTG CATCTGCACT ACTTCCATGA CCATTTTCTG
    GAGCTGACTG ATCTGAAAGC CGTGGTCAGC AAACAGGAGA GCACCATTTA CAGCTATGCT
    AAAGTCAGTG GTCGTATCTA TCTGATCGTG GATAGCATCA AGCAAAAAAT ATCTTCTTCT
    CAGAGCAAAT TGGCTCATCC CGATGGGAGT TTGGCGGATC TGACCATTGT GAGCTCGTCT
    CCGGAGCCGC TGCTGAAGGA TGAGAAGGAA AAAGAGTCTC TGAATGAGGT GCTGGTGGTG
    GATTGTGTGG GGGAAGAGAA GAAGAAAGAC TCTTTATCCA TTTATTCGGC TCAAATGTCC
    AGTCAGGAGG ACGTTCATCA GTCCAGTGCT GCCACAGACC CTGAACTGCC TTCAGAACAA
    AGCTCAGAGA TGAAGAATAA GTGGCATCTG GTGGTGGACC GGCTCACAGT GCTGTTCCTC
    AAGTTCCTGG AGTACTTTCA TAAGCTGAAA CTCTTTGTCT GGTGGCTGCT GGAGATGCAC
    ATCATCAAGA TCGTGGCCAC GTACATCATC CTGCTCTCGG TCAAAGAGGT CTCGCTGCTG
    AACTTTGTGT TTTTGATCTC CTGGGCGTTT GCGCTGCCGT ATAAGCAGTT TCGAGTTCTC
    TCCTCCAGCG TCTGCACCGT ATGGACGTGC GTTATTATTG TCTGCAAGCT CTTTTACCAG
    CTCAAAACCA TCAATCCTGC CAACTACTCC AGTGATTGTA TTATGCCCAA CTATACAGAA
    ACAGAAAAAA AGGACATGAA GACGTCTCTA CTGTATCGTG ATCCAGTGGA TCCTGCTAAC
    TGGGTTGGCC TGCGGAAGTT TGATCATGGG ATAATATTGC CTAACCTGAG GAATAACTTG
    CTGATGTTGG CCCTCTTGGC GTTCGAGGTT ACCATTTACA GACACCAGGA GTTTTTCCGT
    CTAAGAAACA AACTTTCTCC TCCTCCTTCA CGCACCATCT TTCATGACAT CACCCGGCAA
    CACCTTGACA ACGGCATCAT CGAGTGCGCC AAGTACTTTA TTAACTACTT CTTCTACAAG
    TTTGGTTTAG AGGTGTGTTT TTTGTTGGCC ATCAATGTGA TGGGTCAGCG GATGGATTTC
    TACTCCATGG TCCACGGCTT GGCTCTGGCT GTGGTGATGT TCAGACGGCG GAGGAAAGCC
    ATCGCTGAGA TCTGGCCCAA ATACTGCTGC TTCCTGGCCT GTATGATCAC CTTTCAGTAT
    TTCATATGCA TCGGGATTCC ACCGGCTGCT TGTGCAGATT ATCCCTGGAG GTTTCCCAAC
    TCCTCGATGA CGTCTAATGT CATTAAATGG CTTTACCTTC CTGATTTCCA CACTCCACCC
    AGCTCCATGT TCCTCGGCTA TGATTTCATG CTGCTGCTGT GTGCGTCGCT GCAGAGGCAG
    GTGTTTGATG AAGAAAATAA AGCCGCCGTT CGCCTGATGG CGGGAGATAA TGTGGAGATC
    TGCCGAGATC TGGACGCTGC ATCATTCAGT GTTCACAACC CGGTGCCAGA CTTCATTCAC
    TGCAGGTCAT ACCTGGACAT GCTGAAGGTG ATCATGTTCA GTTACCTGTT CTGGTTCGTC
    CTGACCATCA TCTTCATCAC GGGCACCACA CGGATCAGCA TCTTCTGCAT GGGTTACCTG
    GTGGCCTGCT TTTACTTCCT GCTGTTTGGT GGGCAACTTT TACTCAAGCC AATCAAAGAG
    ATCCTCCGTT ACTGGGACTT TCTCATCGCC TACAACGTTT TCGTCATCAC CATGAAGAAC
    GTCTTTGCAA TTTTGGCGTG CGGTTACATC AACTCGCTGG TGAAGAATAG CTGCTGGTTA
    ATTCAGCTGC TCAGTCTGGC CTGCACCATT AAAGACTACC AACCAAAAAC AGATCTGCCT
    GGAGTTGACT GTGAGCTGCC AAAAGACGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    ACCTTCCTCC TCCTGCAGAG AAGAGTCTTC ATGAGCTACT ATTTCTTGCA TGTAATCGCA
    GACATTCGAG CCGGACAGAT TCTTGCATCC CGAGGAGCTG AGTTGTTTCA GGCCAGTATC
    GTGAAGGCTG TGAGAGCTCG TCTGGAGGAG GAGAAGAAAT CCATGGAGCA ACTGAAGAGA
    CAGATGGATC GTATTAAAAC GAGACAGCAG AAGTTCAAGA GAGGAAAAGA GAAGATGCTC
    AGCATAGCCC AAGAGTCTGG AGACGGAGAG AAACTCATCC CACCAGTGGA CAATGAGGAG
    GAGGAGGACG AGGTGGTTAG AAGTGGTGAT TATTACCTGT TTGAAACTGA CAGTGAAGAG
    GAGGAGGAAG AGGAGGAGAA AAAAGACGAA GAGCCTCCCA AAAGATCAGC CTTTCAGCGA
    GCGATCAGAA AGTTTGCTTC AGCCCTTGTT GCTTTACCCA GATCAGTCAT TAAACTGCCC
    AAAACTATTC TGCAGTATTT AATAAGGGCA GCCAAGTTTG TGTATCATAC CTGGATCGCC
    GAATCTAAAG CAGCTCTGAA AGAAAGAGGA AAAGGCAAGC GCTTCTGGAA GCGATACGGT
    CGCCGGCTTA AAAAAGAGAA GAAAGAAGAA CATGTGGCGA TAGAGATCGG TGAAGACGAG
    CGTCAGAGTT CAGAAGAGGA GAAGACAGAT GGACCAGACA ACATCATCAA GAGGGTGTTC
    AACATCATCA AGTTCACCTG GGCTCTGTTT CTGACCACCG TGGAGAGCAT CACCAAATGG
    CTGAACTCAG TCTGCAGAGA ATATATCGAC ATCTCCACCG TCCTGCGCAT CGAGCGCTGC
    ATGCTGACCC GAGAAGTCAA AAAGGGGAAT GTGCCGTCCC GTGAGAGCAT CCATGTGTAC
    TACCAGAAGC AGATGAAGCT GAATGGATCT CGTGAATCTG GTCTGGACCG CATCAGTGAG
    GAGGATTCAG CCTCAAACAG AGAACGCCGC AGACGCGCAC AACACAGTCT GGATTCATTC
    GCTTCCAGAG ACAGCATCTC CAGCGCATAC ACCGAAGCCA CCATGCTGTT TTCCCGCCAG
    TCCACCTTGG ACGATTTGGA CGACATGCCT CAGAATATTC CCAAAACCAG CGAACGCGCT
    CGACCCAAAC TGCGCAAAAT GTACGGCCTC GACATGTCCA ACTCCTCTGC GGACAGCGGA
    AGCAGCGTCA TCTCCAGTGA GGCTACTCAG TGCGTCACGC TGTTTTCTCG GCAAGGAACC
    AACGACACTA TCGACGAGGC TGAAGACGAG CGAGAACAGC TGGAGCACAA GCAGAAAACA
    GAAGAAGCAG AACTCAGAGA CAAACCTGAA GAAGAGCAGG AAGTGGAAAT AAGCGATCAG
    GGGAAACAGG AAGTGTTACC GGTGAGCCAT CTGGTGACAC AGGAAGTGAA AGAGGATGTA
    CTACCTGTGA GCGATCAGCT GACTGGGGAA GTGACTGAGG AAGTTGAAGA GGAGGTGGAA
    GTAGTTAAGG AAATGGAAGA GGAAGTAGAA AAGGAAATTG GAGCAAAGGT GGAAAAGGAA
    GTAGAAGCGG AAAAGGAAGA GGAAGCGGAA AAGGAAGTGG GTGAGGAAGT GCAATGGGAC
    TTGGTGGATC CAGAGGAAGC AGAGGAGTGT GTAAGTGATG GGGACGGTGG TGAGCAGATG
    CAGCAGGATG AAGGTGAATG GTCGGGAGTG GAGAACGTGG TGAGCGGTCC GCTGTTCACA
    CCAGACACTG ACGCCCCCAA CACGTCTGAC GCTGACGTGC CGCCCAGCTA CAGTAAAGCC
    GTGAGCTTCG ACCGGCTGTC TGTGAGCTCG GACGACAGTG ACAGCGACAA ACGGCTGATG
    CTAATGACGC CTGACAGCAA ATCTGACCTG GACGACCCTC TGCTGCCCTC CATGACCACC
    GACATGACCG CCAGCGAACT GCTGCTCAAC AAAATGTTCT ACGACGAGAT GCTGGACAGC
    TCAGATCGCT TCTATAAGTC TCAGCCGCTG GGTCTCCAGT TGTGTTACGC TCTCTACAAC
    CTGCTGGTGG CTCACTCAGA AATGGTGTGT TATCTGGTCA TCATCCTCAA CCACATGATC
    TCCGCCTCCA TGGCCACGCT GGTGCTGCCC ATCCTCATCT TCCTGTGGGC CATGCTGTCC
    GTTCCGCGAC CCAGCAAGAG GTTCTGGATG ACCGCCATCG TCTACACTGA GGTCACCATC
    GTCATCAAAT ACTTCTTCCA GTTCAGCTTT TTCCCTTTCA ATCAGAACCT GGAGGTGAAC
    AAGGGCAAGC CGTACCACCC GCCCAACATC CTGGGCACAG AGAAAAAAGA CGGATACGTG
    CACTACGACC TGGTCCAGCT GCTGGCGCTC TTCTTCCACA GATCCATACT AAAGTGTCAT
    GGTCTCTGGG ACGAGGACGA CCCCAAAGTG CCCCGTAAGG ATGAGTGTCC TCGTCAGGAG
    TCTGTGGATG ACGGGAAGCT GTCGGTGGTC ATGTCACCTG ATGAAGACAG GAAGAGTTCT
    CCGGCGTATT CACTCAAGTC TATGAATCTG GGAATGTCTG TGGACTCATC GCAGGTTCAC
    GTACAGATGC GTTATCCAGA GCAGCGGCCG TACCTGCGCA GACAGAGCAC CAGCGGATCA
    CACTTCTCCC ACAGATCCTC CGCACGCTCC AAACGCGGCA GCACAAGCAC ACGTCACAGC
    ACCCATGGGG AAAACAGCAC AACAGAGTCA GAAGTACCAC AAAAGAGCCG GAAAGAAATG
    ATTATGGAGA AAATCAGAGA GCAGCTCATC AAAGCAAAGG TTTACCTGCT CAAAAGATTG
    GTTGAGTTTT ATCAGCCCAT CCGTCAGTTC TTCTACAACC TGGTTCATCC GGAGTACAGC
    GCCGTGACGG ATGTTTACGT ATTGATGTTT CTAGCAGACA CTGTAGATTT TATCATCATC
    GTCTTTGGCT TTTGGGCATT TGGGAAACAT CAGGGTGGTG CTGACATCAC TTCCTCCCTG
    TCAGAGGACC AGGTTCCTGG GCCGTTCCTT GTTATGGTTC TCATACAGTT TGGGACGATG
    GTAGTGGATC GAGCCCTGTA TCTGCGCAAG ACTGTTGTGG GAAAAGTGAT TTTTCAGGTC
    ATCCTGGTGT TTGGCATCCA TTTCTGGATG TTTTTCATCC TGCCGGGAAT CACAGAGAGG
    CGCTTCAGTC AGAACACTAT CGCTCAGCTC TGGTACTTTG TGAAGTGCAT TTACTTCGGC
    CTGTCAGCCT ATCAGATCAG ATGTGGATAT CCAACCCGCA TCCTGGGAAA CTTCCTCACC
    AAGAGCTACA ACTACGCCAA CCTCTTCCTC TTCCAGGGTT TCCGTCTGAT CCCGTTCCTG
    ACGGAGCTGC GTGCGGTGAT GGACTGGGTC TGGACGGACA CAACGCTCAG CTTGTCCAGC
    TGGATCTGTG TGGAGGACAT CTATGCTCAC ATCTTCATCC TCAAATGCTG GAGGGAGTCT
    GAGAAGCGTT ACCCTCAGCC GCGCGGGCAG AAGAAGAAGA AGGTGGTGAA GTATGGGATG
    GGCGGCATGA TCGTGATGCT CCTGATCTGC ATCGTCTGGT TTCCTCTGCT CTTCATGTCT
    CTGGTCAAAT CTGTGGCAGG AGTGGTTAAC AAGCCTCTGG AGGTGTCCGT CTCGCTCACG
    CTCGGAGGAC TCCAGCCGAT CTTCACAATG AGTGCCCAGC AGAACCATAT CAAATCCGTC
    ACTGGGGCAG AATTTAAGAA GTTCACAGAT CACTACAAGT CTAACGCAAA TGCCATCATG
    TTCCTGGAGT CGTATTTTCC TGAGGATCTG ACAATAGCAC ATCTAGAGGG AAGTTCAAAC
    TCACTCTGGA CCATCAGCCC CCCGAGCAAG GAAAACCTGA AGAATATGCT CAATGACACG
    AGAGAGCCGT TTCCTCTCAC CTTCACCTGG TCCATTCAAA GGAACCTCAG TTTAGGTGCC
    AAAGCTGAAA CTGCAATGGG GAAATACTTC ATTGAGCTGG ACGACGAGAC TAGAAAAGGT
    CTAACTGATC AACTGGGGAA TAGCAGCCAG CGTGTGACAA TCAAAAAAAT ATTTCCAAGG
    TACATCAGAG CACCAAGCGA CTCCAATGCC AAACCTATTG ACCAGCTTTA CAACGATGGA
    GAGTACATGG ACATCACACT GTTTTTAAAT AAGAAGAACA GCTCAGAAGG CGTTCAGGAG
    TGGTGGATCG TCAATCAGAC AGATTCAGGT CCATTACCTG GACTCAACGG GTCTGGTCTT
    CAGATCTACA TCATCAGTGA CCAAGTCAGT CCACCCAGTC TGGGCTTTCT GGCTGGTTAT
    GGGATCATGG GTCTGTACAT GTCAGTGGTT TTGGTGATCG GGAAGTTTGT GCGCGAGTTC
    TTCAGCGGGA TCTCGCACAC CATCATGTTC GAAGAGCTCC CTAACGTGGA CCGAATCCTC
    AAACTCTGCA CCGATATCTT CCTGGTCCGA GAGACGGGCG AACTGGACCT GGAGGAAGAC
    CTTTACGCCA AACTCATCTT CCTCTACCGC TCTCCAGAAA CCATGATCAA ATGGACCAGA
    GAGAAGAGTG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323946.1
    CDS675..9629
    Translation

    Target ORF information:

    RefSeq Version XM_021468271.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio piezo-type mechanosensitive ion channel component 2 (LOC100534973), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468271.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCTTCTG AAGTTGTGTG TGGTTTGGTC TTCAGAGTGC TGTTGCCGGT GTGTCTGGCG 
    GCAGCATGTC TGTTCAGGTT CAATGGGCTG TCCTTCATCT ACCTCCTCTT CCTCCTCCTC
    ATCCCGCTGT TCCCAGAGCC CTCCAGCATC ACCATGAGAG GCCAGACGGG GTACTTGCTG
    AAGTCTTTAT GCTGCTGCAG TGTGACCTTC CTCTTCTTCC ACATCATCTA TCAAATCACC
    GTCCACAGTT TGCTGGCAAG AGGAAGAGTC GATTCTGGAT TCAACTGTTC GGTGTGGGAG
    AAGTCAATAC GACAGATTGG TTTTGAGAGT GTTATCGGGG CAGATGCAGG CAATGGTATC
    CGAGTTTTCC TGCCAGACAT TGGGATGTTC ATGGCCAGTC TGGGTGTGTG GCTCCTGTGC
    CGAAAACTGC TCCACCAACG GCCCACTGAG GATATGGCCC AGGACAACCA GCAATTCCTG
    TCTGAGGAGA AGGAGGAGGA AAACGAGAAG CTGGAAAATG AAGAAGATGA GAATGATAAT
    AATGATGATG ATGATGATGA AATGCTGTTT GACGAGGACT TTGATGCAGA AGATGAAGCA
    GAGGAAGAAG ATGGAGAAGC CGGAAGACTG GAGGAGGAGG AGGAGGAAGA GGAGGAGGAA
    GAGGAAGTGC AGGAGAGCAC CAAGATGAAG ATCCTGCGCG CGGTTGGAGG AGTCGCTGCC
    AAACTCAAGG AGATCATCGG GAACCTCATC ACTACTGCTG GACAGGTGGT GGTCACTATC
    ATGCTGGGAA TGACAGGTGT GACGCTGCCT TCTCTGTCCT CCGCTGTGTA TTTCTTCGTG
    TTTCTGGGTC TGTGCACATG GTGGTCTCTC TGTAAGACCT TCGATAAGCT CTTGTTCAGC
    TGTCTGTGTG TCCTGATGGC CATTTTCAGC GCTGGACATC TCATCGTGCT TTATATCAAC
    CAGTTTCAGT TCCTCCAGGA AGCCATAAGT CCCACCGATG GATATACCAG TGTTTTTGGC
    ATCTCCTCCA TCATTTACAC AGACTGCTCC TCTACATGGA AGCTTGCTGT CAGGAGTGGA
    CTCAAATGGC ATCATTTCGT CAATCCCATA ATGATCCTGG TCCTGTATTA CACACTGGCC
    ACGCTGATCC GCCTGTGGCT GCAGGATCCT ATGATGATGG AGGACAGCGA GGACGGAGAG
    GTGATCGAGG GCAATGAAGC CATGGAAAAC AACGTCCATC ACACGTCCAT CCGGAAACGG
    CTGCTCTGGT GGAAAGCGCA TCAGAAAACC GAGGAGAGAA ATCTGATCTC CACTCAGGAT
    GGGTACAGCA CCTCGGAGGT GTTAGTGGTC ACCTCCAATG GGACGTCGTT TGATTATGTG
    TCCGCCGTGC CCATCGAGAA CGGACCTGTC AGTCTGGACA TTTACTCCAC TCCTCAGTAC
    AAAGTGGATC AGTCTGTCGA ATATGACGAG AAGACGGATG AGAACACAAA TGAGGTGCTG
    GACTCACAGC TGGAGGAGGA AGAGGAGAAG ACAGATGAGG AAGAGGAAGC CGAAGAGGAA
    GAGCAGGGCG GACCACCGGC AGCTCTCGTC AAAGTGTTCA AGTTCATCAT GAAGCAGAGC
    TACATCTGCG CTCTCATCGC TATGATGGCG TGGAGTATAA CGTATGTCAG CTGGCTGACG
    TTTGTCTTTC TGATCTGGTC CTGTACTCTG TGGATGGTGC GCGACCGGAG GAAATACGCC
    ATGCTGACTT CGCCATTCAT GGTGGCTTAT GGGAACTTCC TGTTGGTCCT TCAGTATATC
    TGGTGTTTTG AGAAGTTGAA GCCGGTGTCT GGTTTCTTTG TGAAAATGGA GGTGCCTTTC
    ACTGAACTGA GCACCAAGGT TCTGTGTCAG CTGAGTTTCT GGCTACTGCT CAGACAAACT
    TTAATGGAGA GACAAGAAAG GAAGGAAGAA GAAGCAGCAC TTTCTGACAT CAAAGTAGAC
    GAGCAGAAAA AAGTGGAAGA AAAAAGTGAA GAGGAAGTGG ATGAGAACGA CCTGATGCAA
    GTGCTGGGCA AGCTGGTGAT GGCTCTGCTG GTCAAATACT GGATCTACGT CTGTGGAGGA
    ATGTTCTTCT TCGTCAGTTT TGAGGGAGAT ATGGCGACAT ATAAGATCAT CTACATGATG
    ATGCTGCTGT CCTGCGTCGC TCTTTACCAG GTACATTATG AATACTGGCG CAGGATCCTG
    AAGTATTTCT GGATGTCTGT GGTGGTTTAC ACTATGCTGG TGCTCATCCT GATCTACACC
    AGCCAGTTTG AGGATGGCAT CCCGACCTGG ACCAGGATGA CCGGCCTGGA CAAAGATGTA
    CTGAAGGATA TCGGTCTGGA GAAGTTCAGC CTCTCTAATT TGTTCACGCG TATCTTCATC
    CCGACGTCTT TCCTGTTGGT GTGTATCCTG CATCTGCACT ACTTCCATGA CCATTTTCTG
    GAGCTGACTG ATCTGAAAGC CGTGGTCAGC AAACAGGAGA GCACCATTTA CAGCTATGCT
    AAAGTCAGTG GTCGTATCTA TCTGATCGTG GATAGCATCA AGCAAAAAAT ATCTTCTTCT
    CAGAGCAAAT TGGCTCATCC CGATGGGAGT TTGGCGGATC TGACCATTGT GAGCTCGTCT
    CCGGAGCCGC TGCTGAAGGA TGAGAAGGAA AAAGAGTCTC TGAATGAGGT GCTGGTGGTG
    GATTGTGTGG GGGAAGAGAA GAAGAAAGAC TCTTTATCCA TTTATTCGGC TCAAATGTCC
    AGTCAGGAGG ACGTTCATCA GTCCAGTGCT GCCACAGACC CTGAACTGCC TTCAGAACAA
    AGCTCAGAGA TGAAGAATAA GTGGCATCTG GTGGTGGACC GGCTCACAGT GCTGTTCCTC
    AAGTTCCTGG AGTACTTTCA TAAGCTGAAA CTCTTTGTCT GGTGGCTGCT GGAGATGCAC
    ATCATCAAGA TCGTGGCCAC GTACATCATC CTGCTCTCGG TCAAAGAGGT CTCGCTGCTG
    AACTTTGTGT TTTTGATCTC CTGGGCGTTT GCGCTGCCGT ATAAGCAGTT TCGAGTTCTC
    TCCTCCAGCG TCTGCACCGT ATGGACGTGC GTTATTATTG TCTGCAAGCT CTTTTACCAG
    CTCAAAACCA TCAATCCTGC CAACTACTCC AGTGATTGTA TTATGCCCAA CTATACAGAA
    ACAGAAAAAA AGGACATGAA GACGTCTCTA CTGTATCGTG ATCCAGTGGA TCCTGCTAAC
    TGGGTTGGCC TGCGGAAGTT TGATCATGGG ATAATATTGC CTAACCTGAG GAATAACTTG
    CTGATGTTGG CCCTCTTGGC GTTCGAGGTT ACCATTTACA GACACCAGGA GTTTTTCCGT
    CTAAGAAACA AACTTTCTCC TCCTCCTTCA CGCACCATCT TTCATGACAT CACCCGGCAA
    CACCTTGACA ACGGCATCAT CGAGTGCGCC AAGTACTTTA TTAACTACTT CTTCTACAAG
    TTTGGTTTAG AGGTGTGTTT TTTGTTGGCC ATCAATGTGA TGGGTCAGCG GATGGATTTC
    TACTCCATGG TCCACGGCTT GGCTCTGGCT GTGGTGATGT TCAGACGGCG GAGGAAAGCC
    ATCGCTGAGA TCTGGCCCAA ATACTGCTGC TTCCTGGCCT GTATGATCAC CTTTCAGTAT
    TTCATATGCA TCGGGATTCC ACCGGCTGCT TGTGCAGATT ATCCCTGGAG GTTTCCCAAC
    TCCTCGATGA CGTCTAATGT CATTAAATGG CTTTACCTTC CTGATTTCCA CACTCCACCC
    AGCTCCATGT TCCTCGGCTA TGATTTCATG CTGCTGCTGT GTGCGTCGCT GCAGAGGCAG
    GTGTTTGATG AAGAAAATAA AGCCGCCGTT CGCCTGATGG CGGGAGATAA TGTGGAGATC
    TGCCGAGATC TGGACGCTGC ATCATTCAGT GTTCACAACC CGGTGCCAGA CTTCATTCAC
    TGCAGGTCAT ACCTGGACAT GCTGAAGGTG ATCATGTTCA GTTACCTGTT CTGGTTCGTC
    CTGACCATCA TCTTCATCAC GGGCACCACA CGGATCAGCA TCTTCTGCAT GGGTTACCTG
    GTGGCCTGCT TTTACTTCCT GCTGTTTGGT GGGCAACTTT TACTCAAGCC AATCAAAGAG
    ATCCTCCGTT ACTGGGACTT TCTCATCGCC TACAACGTTT TCGTCATCAC CATGAAGAAC
    GTCTTTGCAA TTTTGGCGTG CGGTTACATC AACTCGCTGG TGAAGAATAG CTGCTGGTTA
    ATTCAGCTGC TCAGTCTGGC CTGCACCATT AAAGACTACC AACCAAAAAC AGATCTGCCT
    GGAGTTGACT GTGAGCTGCC AAAAGACGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    ACCTTCCTCC TCCTGCAGAG AAGAGTCTTC ATGAGCTACT ATTTCTTGCA TGTAATCGCA
    GACATTCGAG CCGGACAGAT TCTTGCATCC CGAGGAGCTG AGTTGTTTCA GGCCAGTATC
    GTGAAGGCTG TGAGAGCTCG TCTGGAGGAG GAGAAGAAAT CCATGGAGCA ACTGAAGAGA
    CAGATGGATC GTATTAAAAC GAGACAGCAG AAGTTCAAGA GAGGAAAAGA GAAGATGCTC
    AGCATAGCCC AAGAGTCTGG AGACGGAGAG AAACTCATCC CACCAGTGGA CAATGAGGAG
    GAGGAGGACG AGGTGGTTAG AAGTGGTGAT TATTACCTGT TTGAAACTGA CAGTGAAGAG
    GAGGAGGAAG AGGAGGAGAA AAAAGACGAA GAGCCTCCCA AAAGATCAGC CTTTCAGCGA
    GCGATCAGAA AGTTTGCTTC AGCCCTTGTT GCTTTACCCA GATCAGTCAT TAAACTGCCC
    AAAACTATTC TGCAGTATTT AATAAGGGCA GCCAAGTTTG TGTATCATAC CTGGATCGCC
    GAATCTAAAG CAGCTCTGAA AGAAAGAGGA AAAGGCAAGC GCTTCTGGAA GCGATACGGT
    CGCCGGCTTA AAAAAGAGAA GAAAGAAGAA CATGTGGCGA TAGAGATCGG TGAAGACGAG
    CGTCAGAGTT CAGAAGAGGA GAAGACAGAT GGACCAGACA ACATCATCAA GAGGGTGTTC
    AACATCATCA AGTTCACCTG GGCTCTGTTT CTGACCACCG TGGAGAGCAT CACCAAATGG
    CTGAACTCAG TCTGCAGAGA ATATATCGAC ATCTCCACCG TCCTGCGCAT CGAGCGCTGC
    ATGCTGACCC GAGAAGTCAA AAAGGGGAAT GTGCCGTCCC GTGAGAGCAT CCATGTGTAC
    TACCAGAAGC AGATGAAGCT GAATGGATCT CGTGAATCTG GTCTGGACCG CATCAGTGAG
    GAGGATTCAG CCTCAAACAG AGAACGCCGC AGACGCGCAC AACACAGTCT GGATTCATTC
    GCTTCCAGAG ACAGCATCTC CAGCGCATAC ACCGAAGCCA CCATGCTGTT TTCCCGCCAG
    TCCACCTTGG ACGATTTGGA CGACATGCCT CAGAATATTC CCAAAACCAG CGAACGCGCT
    CGACCCAAAC TGCGCAAAAT GTACGGCCTC GACATGTCCA ACTCCTCTGC GGACAGCGGA
    AGCAGCGTCA TCTCCAGTGA GGCTACTCAG TGCGTCACGC TGTTTTCTCG GCAAGGAACC
    AACGACACTA TCGACGAGGC TGAAGACGAG CGAGAACAGC TGGAGCACAA GCAGAAAACA
    GAAGAAGCAG AACTCAGAGA CAAACCTGAA GAAGAGCAGG AAGTGGAAAT AAGCGATCAG
    GGGAAACAGG AAGTGTTACC GGTGAGCCAT CTGGTGACAC AGGAAGTGAA AGAGGATGTA
    CTACCTGTGA GCGATCAGCT GACTGGGGAA GTGACTGAGG AAGTTGAAGA GGAGGTGGAA
    GTAGTTAAGG AAATGGAAGA GGAAGTAGAA AAGGAAATTG GAGCAAAGGT GGAAAAGGAA
    GTAGAAGCGG AAAAGGAAGA GGAAGCGGAA AAGGAAGTGG GTGAGGAAGT GCAATGGGAC
    TTGGTGGATC CAGAGGAAGC AGAGGAGTGT GTAAGTGATG GGGACGGTGG TGAGCAGATG
    CAGCAGGATG AAGGTGAATG GTCGGGAGTG GAGAACGTGG TGAGCGGTCC GCTGTTCACA
    CCAGACACTG ACGCCCCCAA CACGTCTGAC GCTGACGTGC CGCCCAGCTA CAGTAAAGCC
    GTGAGCTTCG ACCGGCTGTC TGTGAGCTCG GACGACAGTG ACAGCGACAA ACGGCTGATG
    CTAATGACGC CTGACAGCAA ATCTGACCTG GACGACCCTC TGCTGCCCTC CATGACCACC
    GACATGACCG CCAGCGAACT GCTGCTCAAC AAAATGTTCT ACGACGAGAT GCTGGACAGC
    TCAGATCGCT TCTATAAGTC TCAGCCGCTG GGTCTCCAGT TGTGTTACGC TCTCTACAAC
    CTGCTGGTGG CTCACTCAGA AATGGTGTGT TATCTGGTCA TCATCCTCAA CCACATGATC
    TCCGCCTCCA TGGCCACGCT GGTGCTGCCC ATCCTCATCT TCCTGTGGGC CATGCTGTCC
    GTTCCGCGAC CCAGCAAGAG GTTCTGGATG ACCGCCATCG TCTACACTGA GGTCACCATC
    GTCATCAAAT ACTTCTTCCA GTTCAGCTTT TTCCCTTTCA ATCAGAACCT GGAGGTGAAC
    AAGGGCAAGC CGTACCACCC GCCCAACATC CTGGGCACAG AGAAAAAAGA CGGATACGTG
    CACTACGACC TGGTCCAGCT GCTGGCGCTC TTCTTCCACA GATCCATACT AAAGTGTCAT
    GGTCTCTGGG ACGAGGACGA CCCCAAAGTG CCCCGTAAGG ATGAGTGTCC TCGTCAGGAG
    TCTGTGGATG ACGGGAAGCT GTCGGTGGTC ATGTCACCTG ATGAAGACAG GAAGAGTTCT
    CCGGCGTATT CACTCAAGTC TATGAATCTG GGAATGTCTG TGGACTCATC GCAGGTTCAC
    GTACAGATGC GTTATCCAGA GCAGCGGCCG TACCTGCGCA GACAGAGCAC CAGCGGATCA
    CACTTCTCCC ACAGATCCTC CGCACGCTCC AAACGCGGCA GCACAAGCAC ACGTCACAGC
    ACCCATGGGG AAAACAGCAC AACAGAGTCA GAAGTACCAC AAAAGAGCCG GAAAGAAATG
    ATTATGGAGA AAATCAGAGA GCAGCTCATC AAAGCAAAGG TTTACCTGCT CAAAAGATTG
    GTTGAGTTTT ATCAGCCCAT CCGTCAGTTC TTCTACAACC TGGTTCATCC GGAGTACAGC
    GCCGTGACGG ATGTTTACGT ATTGATGTTT CTAGCAGACA CTGTAGATTT TATCATCATC
    GTCTTTGGCT TTTGGGCATT TGGGAAACAT CAGGGTGGTG CTGACATCAC TTCCTCCCTG
    TCAGAGGACC AGGTTCCTGG GCCGTTCCTT GTTATGGTTC TCATACAGTT TGGGACGATG
    GTAGTGGATC GAGCCCTGTA TCTGCGCAAG ACTGTTGTGG GAAAAGTGAT TTTTCAGGTC
    ATCCTGGTGT TTGGCATCCA TTTCTGGATG TTTTTCATCC TGCCGGGAAT CACAGAGAGG
    CGCTTCAGTC AGAACACTAT CGCTCAGCTC TGGTACTTTG TGAAGTGCAT TTACTTCGGC
    CTGTCAGCCT ATCAGATCAG ATGTGGATAT CCAACCCGCA TCCTGGGAAA CTTCCTCACC
    AAGAGCTACA ACTACGCCAA CCTCTTCCTC TTCCAGGGTT TCCGTCTGAT CCCGTTCCTG
    ACGGAGCTGC GTGCGGTGAT GGACTGGGTC TGGACGGACA CAACGCTCAG CTTGTCCAGC
    TGGATCTGTG TGGAGGACAT CTATGCTCAC ATCTTCATCC TCAAATGCTG GAGGGAGTCT
    GAGAAGCGTT ACCCTCAGCC GCGCGGGCAG AAGAAGAAGA AGGTGGTGAA GTATGGGATG
    GGCGGCATGA TCGTGATGCT CCTGATCTGC ATCGTCTGGT TTCCTCTGCT CTTCATGTCT
    CTGGTCAAAT CTGTGGCAGG AGTGGTTAAC AAGCCTCTGG AGGTGTCCGT CTCGCTCACG
    CTCGGAGGAC TCCAGCCGAT CTTCACAATG AGTGCCCAGC AGAACCATAT CAAATCCGTC
    ACTGGGGCAG AATTTAAGAA GTTCACAGAT CACTACAAGT CTAACGCAAA TGCCATCATG
    TTCCTGGAGT CGTATTTTCC TGAGGATCTG ACAATAGCAC ATCTAGAGGG AAGTTCAAAC
    TCACTCTGGA CCATCAGCCC CCCGAGCAAG GAAAACCTGA AGAATATGCT CAATGACACG
    AGAGAGCCGT TTCCTCTCAC CTTCACCTGG TCCATTCAAA GGAACCTCAG TTTAGGTGCC
    AAAGCTGAAA CTGCAATGGG GAAATACTTC ATTGAGCTGG ACGACGAGAC TAGAAAAGGT
    CTAACTGATC AACTGGGGAA TAGCAGCCAG CGTGTGACAA TCAAAAAAAT ATTTCCAAGG
    TACATCAGAG CACCAAGCGA CTCCAATGCC AAACCTATTG ACCAGCTTTA CAACGATGGA
    GAGTACATGG ACATCACACT GTTTTTAAAT AAGAAGAACA GCTCAGAAGG CGTTCAGGAG
    TGGTGGATCG TCAATCAGAC AGATTCAGGT CCATTACCTG GACTCAACGG GTCTGGTCTT
    CAGATCTACA TCATCAGTGA CCAAGTCAGT CCACCCAGTC TGGGCTTTCT GGCTGGTTAT
    GGGATCATGG GTCTGTACAT GTCAGTGGTT TTGGTGATCG GGAAGTTTGT GCGCGAGTTC
    TTCAGCGGGA TCTCGCACAC CATCATGTTC GAAGAGCTCC CTAACGTGGA CCGAATCCTC
    AAACTCTGCA CCGATATCTT CCTGGTCCGA GAGACGGGCG AACTGGACCT GGAGGAAGAC
    CTTTACGCCA AACTCATCTT CCTCTACCGC TCTCCAGAAA CCATGATCAA ATGGACCAGA
    GAGAAGAGTG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150520
    Clone ID Related Accession (Same CDS sequence) XM_017352447.2
    Accession Version XM_017352447.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCTTCTG AAGTTGTGTG TGGTTTGGTC TTCAGAGTGC TGTTGCCGGT GTGTCTGGCG 
    GCAGCATGTC TGTTCAGGTT CAATGGGCTG TCCTTCATCT ACCTCCTCTT CCTCCTCCTC
    ATCCCGCTGT TCCCAGAGCC CTCCAGCATC ACCATGAGAG GCCAGACGGG GTACTTGCTG
    AAGTCTTTAT GCTGCTGCAG TGTGACCTTC CTCTTCTTCC ACATCATCTA TCAAATCACC
    GTCCACAGTT TGCTGGCAAG AGGAAGAGTC GATTCTGGAT TCAACTGTTC GGTGTGGGAG
    AAGTCAATAC GACAGATTGG TTTTGAGAGT GTTATCGGGG CAGATGCAGG CAATGGTATC
    CGAGTTTTCC TGCCAGACAT TGGGATGTTC ATGGCCAGTC TGGGTGTGTG GCTCCTGTGC
    CGAAAACTGC TCCACCAACG GCCCACTGAG GATATGGCCC AGGACAACCA GCAATTCCTG
    TCTGAGGAGA AGGAGGAGGA AAACGAGAAG CTGGAAAATG AAGAAGATGA GAATGATAAT
    AATGATGATG ATGATGATGA AATGCTGTTT GACGAGGACT TTGATGCAGA AGATGAAGCA
    GAGGAAGAAG ATGGAGAAGC CGGAAGACTG GAGGAGGAGG AGGAGGAAGA GGAGGAGGAA
    GAGGAAGTGC AGGAGAGCAC CAAGATGAAG ATCCTGCGCG CGGTTGGAGG AGTCGCTGCC
    AAACTCAAGG AGATCATCGG GAACCTCATC ACTACTGCTG GACAGGTGGT GGTCACTATC
    ATGCTGGGAA TGACAGGTGT GACGCTGCCT TCTCTGTCCT CCGCTGTGTA TTTCTTCGTG
    TTTCTGGGTC TGTGCACATG GTGGTCTCTC TGTAAGACCT TCGATAAGCT CTTGTTCAGC
    TGTCTGTGTG TCCTGATGGC CATTTTCAGC GCTGGACATC TCATCGTGCT TTATATCAAC
    CAGTTTCAGT TCCTCCAGGA AGCCATAAGT CCCACCGATG GATATACCAG TGTTTTTGGC
    ATCTCCTCCA TCATTTACAC AGACTGCTCC TCTACATGGA AGCTTGCTGT CAGGAGTGGA
    CTCAAATGGC ATCATTTCGT CAATCCCATA ATGATCCTGG TCCTGTATTA CACACTGGCC
    ACGCTGATCC GCCTGTGGCT GCAGGATCCT ATGATGATGG AGGACAGCGA GGACGGAGAG
    GTGATCGAGG GCAATGAAGC CATGGAAAAC AACGTCCATC ACACGTCCAT CCGGAAACGG
    CTGCTCTGGT GGAAAGCGCA TCAGAAAACC GAGGAGAGAA ATCTGATCTC CACTCAGGAT
    GGGTACAGCA CCTCGGAGGT GTTAGTGGTC ACCTCCAATG GGACGTCGTT TGATTATGTG
    TCCGCCGTGC CCATCGAGAA CGGACCTGTC AGTCTGGACA TTTACTCCAC TCCTCAGTAC
    AAAGTGGATC AGTCTGTCGA ATATGACGAG AAGACGGATG AGAACACAAA TGAGGTGCTG
    GACTCACAGC TGGAGGAGGA AGAGGAGAAG ACAGATGAGG AAGAGGAAGC CGAAGAGGAA
    GAGCAGGGCG GACCACCGGC AGCTCTCGTC AAAGTGTTCA AGTTCATCAT GAAGCAGAGC
    TACATCTGCG CTCTCATCGC TATGATGGCG TGGAGTATAA CGTATGTCAG CTGGCTGACG
    TTTGTCTTTC TGATCTGGTC CTGTACTCTG TGGATGGTGC GCGACCGGAG GAAATACGCC
    ATGCTGACTT CGCCATTCAT GGTGGCTTAT GGGAACTTCC TGTTGGTCCT TCAGTATATC
    TGGTGTTTTG AGAAGTTGAA GCCGGTGTCT GGTTTCTTTG TGAAAATGGA GGTGCCTTTC
    ACTGAACTGA GCACCAAGGT TCTGTGTCAG CTGAGTTTCT GGCTACTGCT CAGACAAACT
    TTAATGGAGA GACAAGAAAG GAAGGAAGAA GAAGCAGCAC TTTCTGACAT CAAAGTAGAC
    GAGCAGAAAA AAGTGGAAGA AAAAAGTGAA GAGGAAGTGG ATGAGAACGA CCTGATGCAA
    GTGCTGGGCA AGCTGGTGAT GGCTCTGCTG GTCAAATACT GGATCTACGT CTGTGGAGGA
    ATGTTCTTCT TCGTCAGTTT TGAGGGAGAT ATGGCGACAT ATAAGATCAT CTACATGATG
    ATGCTGCTGT CCTGCGTCGC TCTTTACCAG GTACATTATG AATACTGGCG CAGGATCCTG
    AAGTATTTCT GGATGTCTGT GGTGGTTTAC ACTATGCTGG TGCTCATCCT GATCTACACC
    AGCCAGTTTG AGGATGGCAT CCCGACCTGG ACCAGGATGA CCGGCCTGGA CAAAGATGTA
    CTGAAGGATA TCGGTCTGGA GAAGTTCAGC CTCTCTAATT TGTTCACGCG TATCTTCATC
    CCGACGTCTT TCCTGTTGGT GTGTATCCTG CATCTGCACT ACTTCCATGA CCATTTTCTG
    GAGCTGACTG ATCTGAAAGC CGTGGTCAGC AAACAGGAGA GCACCATTTA CAGATTGGCT
    CATCCCGATG GGAGTTTGGC GGATCTGACC ATTGTGAGCT CGTCTCCGGA GCCGCTGCTG
    AAGGATGAGA AGGAAAAAGA GTCTCTGAAT GAGGTGCTGG TGGTGGATTG TGTGGGGGAA
    GAGAAGAAGA AAGACTCTTT ATCCATTTAT TCGGCTCAAA TGTCCAGTCA GGAGGACGTT
    CATCAGTCCA GTGCTGCCAC AGACCCTGAA CTGCCTTCAG AACAAAGCTC AGAGATGAAG
    AATAAGTGGC ATCTGGTGGT GGACCGGCTC ACAGTGCTGT TCCTCAAGTT CCTGGAGTAC
    TTTCATAAGC TGAAACTCTT TGTCTGGTGG CTGCTGGAGA TGCACATCAT CAAGATCGTG
    GCCACGTACA TCATCCTGCT CTCGGTCAAA GAGGTCTCGC TGCTGAACTT TGTGTTTTTG
    ATCTCCTGGG CGTTTGCGCT GCCGTATAAG CAGTTTCGAG TTCTCTCCTC CAGCGTCTGC
    ACCGTATGGA CGTGCGTTAT TATTGTCTGC AAGCTCTTTT ACCAGCTCAA AACCATCAAT
    CCTGCCAACT ACTCCAGTGA TTGTATTATG CCCAACTATA CAGAAACAGA AAAAAAGGAC
    ATGAAGACGT CTCTACTGTA TCGTGATCCA GTGGATCCTG CTAACTGGGT TGGCCTGCGG
    AAGTTTGATC ATGGGATAAT ATTGCCTAAC CTGAGGAATA ACTTGCTGAT GTTGGCCCTC
    TTGGCGTTCG AGGTTACCAT TTACAGACAC CAGGAGTTTT TCCGTCTAAG AAACAAACTT
    TCTCCTCCTC CTTCACGCAC CATCTTTCAT GACATCACCC GGCAACACCT TGACAACGGC
    ATCATCGAGT GCGCCAAGTA CTTTATTAAC TACTTCTTCT ACAAGTTTGG TTTAGAGGTG
    TGTTTTTTGT TGGCCATCAA TGTGATGGGT CAGCGGATGG ATTTCTACTC CATGGTCCAC
    GGCTTGGCTC TGGCTGTGGT GATGTTCAGA CGGCGGAGGA AAGCCATCGC TGAGATCTGG
    CCCAAATACT GCTGCTTCCT GGCCTGTATG ATCACCTTTC AGTATTTCAT ATGCATCGGG
    ATTCCACCGG CTGCTTGTGC AGATTATCCC TGGAGGTTTC CCAACTCCTC GATGACGTCT
    AATGTCATTA AATGGCTTTA CCTTCCTGAT TTCCACACTC CACCCAGCTC CATGTTCCTC
    GGCTATGATT TCATGCTGCT GCTGTGTGCG TCGCTGCAGA GGCAGGTGTT TGATGAAGAA
    AATAAAGCCG CCGTTCGCCT GATGGCGGGA GATAATGTGG AGATCTGCCG AGATCTGGAC
    GCTGCATCAT TCAGTGTTCA CAACCCGGTG CCAGACTTCA TTCACTGCAG GTCATACCTG
    GACATGCTGA AGGTGATCAT GTTCAGTTAC CTGTTCTGGT TCGTCCTGAC CATCATCTTC
    ATCACGGGCA CCACACGGAT CAGCATCTTC TGCATGGGTT ACCTGGTGGC CTGCTTTTAC
    TTCCTGCTGT TTGGTGGGCA ACTTTTACTC AAGCCAATCA AAGAGATCCT CCGTTACTGG
    GACTTTCTCA TCGCCTACAA CGTTTTCGTC ATCACCATGA AGAACGTCTT TGCAATTTTG
    GCGTGCGGTT ACATCAACTC GCTGGTGAAG AATAGCTGCT GGTTAATTCA GCTGCTCAGT
    CTGGCCTGCA CCATTAAAGA CTACCAACCA AAAACAGATC TGCCTGGAGT TGACTGTGAG
    CTGCCAAAAG ACGAGGCCGG GATCATCTGG GACAGCATCT GCTTCACCTT CCTCCTCCTG
    CAGAGAAGAG TCTTCATGAG CTACTATTTC TTGCATGTAA TCGCAGACAT TCGAGCCGGA
    CAGATTCTTG CATCCCGAGG AGCTGAGTTG TTTCAGGCCA GTATCGTGAA GGCTGTGAGA
    GCTCGTCTGG AGGAGGAGAA GAAATCCATG GAGCAACTGA AGAGACAGAT GGATCGTATT
    AAAACGAGAC AGCAGAAGTT CAAGAGAGGA AAAGAGAAGA TGCTCAGCAT AGCCCAAGAG
    TCTGGAGACG GAGAGAAACT CATCCCACCA GTGGACAATG AGGAGGAGGA GGACGAGGAG
    AAAAAGAAAA ACAAGGTGCA AAAAAAGCAG TGGTGGAGGC CTTGGGTTGA CCATGCGTCC
    ATGGTTAGAA GTGGTGATTA TTACCTGTTT GAAACTGACA GTGAAGAGGA GGAGGAAGAG
    GAGGAGAAAA AAGACGAAGA GCCTCCCAAA AGATCAGCCT TTCAGCGAGC GATCAGAAAG
    TTTGCTTCAG CCCTTGTTGC TTTACCCAGA TCAGTCATTA AACTGCCCAA AACTATTCTG
    CAGTATTTAA TAAGGGCAGC CAAGTTTGTG TATCATACCT GGATCGCCGA ATCTAAAGCA
    GCTCTGAAAG AAAGAGGAAA AGGCAAGCGC TTCTGGAAGC GATACGGTCG CCGGCTTAAA
    AAAGAGAAGA AAGAAGAACA TGTGGCGATA GAGATCGGTG AAGACGAGCG TCAGAGTTCA
    GAAGAGGAGA AGACAGATGG ACCAGACAAC ATCATCAAGA GGGTGTTCAA CATCATCAAG
    TTCACCTGGG CTCTGTTTCT GACCACCGTG GAGAGCATCA CCAAATGGCT GAACTCAGTC
    TGCAGAGAAT ATATCGACAT CTCCACCGTC CTGCGCATCG AGCGCTGCAT GCTGACCCGA
    GAAGTCAAAA AGGGGAATGT GCCGTCCCGT GAGAGCATCC ATGTGTACTA CCAGAAGCAG
    ATGAAGCTGA ATGGATCTCG TGAATCTGGT CTGGACCGCA TCAGTGAGGA GGATTCAGCC
    TCAAACAGAG AACGCCGCAG ACGCGCACAA CACAGTCTGG ATTCATTCGC TTCCAGAGAC
    AGCATCTCCA GCGCATACAC CGAAGCCACC ATGCTGTTTT CCCGCCAGTC CACCTTGGAC
    GATTTGGACG ACATGCCTCA GAATATTCCC AAAACCAGCG AACGCGCTCG ACCCAAACTG
    CGCAAAATGT ACGGCCTCGA CATGTCCAAC TCCTCTGCGG ACAGCGGAAG CAGCGTCATC
    TCCAGTGAGG CTACTCAGTG CGTCACGCTG TTTTCTCGGC AAGGAACCAA CGACACTATC
    GACGAGGCTG AAGACGAGCG AGAACAGCTG GAGCACAAGC AGAAAACAGA AGAAGCAGAA
    CTCAGAGACA AACCTGAAGA AGAGCAGGAA GTGGAAATAA GCGATCAGGG GAAACAGGAA
    GTGTTACCGG TGAGCCATCT GGTGACACAG GAAGTGAAAG AGGATGTACT ACCTGTGAGC
    GATCAGCTGA CTGGGGAAGT GACTGAGGAA GTTGAAGAGG AGGTGGAAGT AGTTAAGGAA
    ATGGAAGAGG AAGTAGAAAA GGAAATTGGA GCAAAGGTGG AAAAGGAAGT AGAAGCGGAA
    AAGGAAGAGG AAGCGGAAAA GGAAGTGGGT GAGGAAGTGC AATGGGACTT GGTGGATCCA
    GAGGAAGCAG AGGAGTGTGT AAGTGATGGG GACGGTGGTG AGCAGATGCA GCAGGATGAA
    GGTGAATGGT CGGGAGTGGA GAACGTGGTG AGCGGTCCGC TGTTCACACC AGACACTGAC
    GCCCCCAACA CGTCTGACGC TGACGTGCCG CCCAGCTACA GTAAAGCCGT GAGCTTCGAC
    CGGCTGTCTG TGAGCTCGGA CGACAGTGAC AGCGACAAAC GGCTGATGCT AATGACGCCT
    GACAGCAAAT CTGACCTGGA CGACCCTCTG CTGCCCTCCA TGACCACCGA CATGACCGCC
    AGCGAACTGC TGCTCAACAA AATGTTCTAC GACGAGATGC TGGACAGCTC AGATCGCTTC
    TATAAGTCTC AGCCGCTGGG TCTCCAGTTG TGTTACGCTC TCTACAACCT GCTGGTGGCT
    CACTCAGAAA TGGTGTGTTA TCTGGTCATC ATCCTCAACC ACATGATCTC CGCCTCCATG
    GCCACGCTGG TGCTGCCCAT CCTCATCTTC CTGTGGGCCA TGCTGTCCGT TCCGCGACCC
    AGCAAGAGGT TCTGGATGAC CGCCATCGTC TACACTGAGG TCACCATCGT CATCAAATAC
    TTCTTCCAGT TCAGCTTTTT CCCTTTCAAT CAGAACCTGG AGGTGAACAA GGGCAAGCCG
    TACCACCCGC CCAACATCCT GGGCACAGAG AAAAAAGACG GATACGTGCA CTACGACCTG
    GTCCAGCTGC TGGCGCTCTT CTTCCACAGA TCCATACTAA AGTGTCATGG TCTCTGGGAC
    GAGGACGACC CCAAAGTGCC CCGTAAGGAT GAGTGTCCTC GTCAGGAGTC TGTGGATGAC
    GGGAAGCTGT CGGTGGTCAT GTCACCTGAT GAAGACAGGA AGAGTTCTCC GGCGTATTCA
    CTCAAGTCTA TGAATCTGGG AATGTCTGTG GACTCATCGC AGGTTCACGT ACAGATGCGT
    TATCCAGAGC AGCGGCCGTA CCTGCGCAGA CAGAGCACCA GCGGATCACA CTTCTCCCAC
    AGATCCTCCG CACGCTCCAA ACGCGGCAGC ACAAGCACAC GTCACAGCAC CCATGGGGAA
    AACAGCACAA CAGAGTCAGA AGTACCACAA AAGAGCCGGA AAGAAATGAT TATGGAGAAA
    ATCAGAGAGC AGCTCATCAA AGCAAAGGTT TACCTGCTCA AAAGATTGGT TGAGTTTTAT
    CAGCCCATCC GTCAGTTCTT CTACAACCTG GTTCATCCGG AGTACAGCGC CGTGACGGAT
    GTTTACGTAT TGATGTTTCT AGCAGACACT GTAGATTTTA TCATCATCGT CTTTGGCTTT
    TGGGCATTTG GGAAACATCA GGGTGGTGCT GACATCACTT CCTCCCTGTC AGAGGACCAG
    GTTCCTGGGC CGTTCCTTGT TATGGTTCTC ATACAGTTTG GGACGATGGT AGTGGATCGA
    GCCCTGTATC TGCGCAAGAC TGTTGTGGGA AAAGTGATTT TTCAGGTCAT CCTGGTGTTT
    GGCATCCATT TCTGGATGTT TTTCATCCTG CCGGGAATCA CAGAGAGGCG CTTCAGTCAG
    AACACTATCG CTCAGCTCTG GTACTTTGTG AAGTGCATTT ACTTCGGCCT GTCAGCCTAT
    CAGATCAGAT GTGGATATCC AACCCGCATC CTGGGAAACT TCCTCACCAA GAGCTACAAC
    TACGCCAACC TCTTCCTCTT CCAGGGTTTC CGTCTGATCC CGTTCCTGAC GGAGCTGCGT
    GCGGTGATGG ACTGGGTCTG GACGGACACA ACGCTCAGCT TGTCCAGCTG GATCTGTGTG
    GAGGACATCT ATGCTCACAT CTTCATCCTC AAATGCTGGA GGGAGTCTGA GAAGCGTTAC
    CCTCAGCCGC GCGGGCAGAA GAAGAAGAAG GTGGTGAAGT ATGGGATGGG CGGCATGATC
    GTGATGCTCC TGATCTGCAT CGTCTGGTTT CCTCTGCTCT TCATGTCTCT GGTCAAATCT
    GTGGCAGGAG TGGTTAACAA GCCTCTGGAG GTGTCCGTCT CGCTCACGCT CGGAGGACTC
    CAGCCGATCT TCACAATGAG TGCCCAGCAG AACCATATCA AATCCGTCAC TGGGGCAGAA
    TTTAAGAAGT TCACAGATCA CTACAAGTCT AACGCAAATG CCATCATGTT CCTGGAGTCG
    TATTTTCCTG AGGATCTGAC AATAGCACAT CTAGAGGGAA GTTCAAACTC ACTCTGGACC
    ATCAGCCCCC CGAGCAAGGA AAACCTGAAG AATATGCTCA ATGACACGAG AGAGCCGTTT
    CCTCTCACCT TCACCTGGTC CATTCAAAGG AACCTCAGTT TAGGTGCCAA AGCTGAAACT
    GCAATGGGGA AATACTTCAT TGAGCTGGAC GACGAGACTA GAAAAGGTCT AACTGATCAA
    CTGGGGAATA GCAGCCAGCG TGTGACAATC AAAAAAATAT TTCCAAGGTA CATCAGAGCA
    CCAAGCGACT CCAATGCCAA ACCTATTGAC CAGCTTTACA ACGATGGAGA GTACATGGAC
    ATCACACTGT TTTTAAATAA GAAGAACAGC TCAGAAGGCG TTCAGGAGTG GTGGATCGTC
    AATCAGACAG ATTCAGGTCC ATTACCTGGA CTCAACGGGT CTGGTCTTCA GATCTACATC
    ATCAGTGACC AAGTCAGTCC ACCCAGTCTG GGCTTTCTGG CTGGTTATGG GATCATGGGT
    CTGTACATGT CAGTGGTTTT GGTGATCGGG AAGTTTGTGC GCGAGTTCTT CAGCGGGATC
    TCGCACACCA TCATGTTCGA AGAGCTCCCT AACGTGGACC GAATCCTCAA ACTCTGCACC
    GATATCTTCC TGGTCCGAGA GACGGGCGAA CTGGACCTGG AGGAAGACCT TTACGCCAAA
    CTCATCTTCC TCTACCGCTC TCCAGAAACC ATGATCAAAT GGACCAGAGA GAAGAGTGAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207936.2
    CDS675..9617
    Translation

    Target ORF information:

    RefSeq Version XM_017352447.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio piezo-type mechanosensitive ion channel component 2 (LOC100534973), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352447.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCTTCTG AAGTTGTGTG TGGTTTGGTC TTCAGAGTGC TGTTGCCGGT GTGTCTGGCG 
    GCAGCATGTC TGTTCAGGTT CAATGGGCTG TCCTTCATCT ACCTCCTCTT CCTCCTCCTC
    ATCCCGCTGT TCCCAGAGCC CTCCAGCATC ACCATGAGAG GCCAGACGGG GTACTTGCTG
    AAGTCTTTAT GCTGCTGCAG TGTGACCTTC CTCTTCTTCC ACATCATCTA TCAAATCACC
    GTCCACAGTT TGCTGGCAAG AGGAAGAGTC GATTCTGGAT TCAACTGTTC GGTGTGGGAG
    AAGTCAATAC GACAGATTGG TTTTGAGAGT GTTATCGGGG CAGATGCAGG CAATGGTATC
    CGAGTTTTCC TGCCAGACAT TGGGATGTTC ATGGCCAGTC TGGGTGTGTG GCTCCTGTGC
    CGAAAACTGC TCCACCAACG GCCCACTGAG GATATGGCCC AGGACAACCA GCAATTCCTG
    TCTGAGGAGA AGGAGGAGGA AAACGAGAAG CTGGAAAATG AAGAAGATGA GAATGATAAT
    AATGATGATG ATGATGATGA AATGCTGTTT GACGAGGACT TTGATGCAGA AGATGAAGCA
    GAGGAAGAAG ATGGAGAAGC CGGAAGACTG GAGGAGGAGG AGGAGGAAGA GGAGGAGGAA
    GAGGAAGTGC AGGAGAGCAC CAAGATGAAG ATCCTGCGCG CGGTTGGAGG AGTCGCTGCC
    AAACTCAAGG AGATCATCGG GAACCTCATC ACTACTGCTG GACAGGTGGT GGTCACTATC
    ATGCTGGGAA TGACAGGTGT GACGCTGCCT TCTCTGTCCT CCGCTGTGTA TTTCTTCGTG
    TTTCTGGGTC TGTGCACATG GTGGTCTCTC TGTAAGACCT TCGATAAGCT CTTGTTCAGC
    TGTCTGTGTG TCCTGATGGC CATTTTCAGC GCTGGACATC TCATCGTGCT TTATATCAAC
    CAGTTTCAGT TCCTCCAGGA AGCCATAAGT CCCACCGATG GATATACCAG TGTTTTTGGC
    ATCTCCTCCA TCATTTACAC AGACTGCTCC TCTACATGGA AGCTTGCTGT CAGGAGTGGA
    CTCAAATGGC ATCATTTCGT CAATCCCATA ATGATCCTGG TCCTGTATTA CACACTGGCC
    ACGCTGATCC GCCTGTGGCT GCAGGATCCT ATGATGATGG AGGACAGCGA GGACGGAGAG
    GTGATCGAGG GCAATGAAGC CATGGAAAAC AACGTCCATC ACACGTCCAT CCGGAAACGG
    CTGCTCTGGT GGAAAGCGCA TCAGAAAACC GAGGAGAGAA ATCTGATCTC CACTCAGGAT
    GGGTACAGCA CCTCGGAGGT GTTAGTGGTC ACCTCCAATG GGACGTCGTT TGATTATGTG
    TCCGCCGTGC CCATCGAGAA CGGACCTGTC AGTCTGGACA TTTACTCCAC TCCTCAGTAC
    AAAGTGGATC AGTCTGTCGA ATATGACGAG AAGACGGATG AGAACACAAA TGAGGTGCTG
    GACTCACAGC TGGAGGAGGA AGAGGAGAAG ACAGATGAGG AAGAGGAAGC CGAAGAGGAA
    GAGCAGGGCG GACCACCGGC AGCTCTCGTC AAAGTGTTCA AGTTCATCAT GAAGCAGAGC
    TACATCTGCG CTCTCATCGC TATGATGGCG TGGAGTATAA CGTATGTCAG CTGGCTGACG
    TTTGTCTTTC TGATCTGGTC CTGTACTCTG TGGATGGTGC GCGACCGGAG GAAATACGCC
    ATGCTGACTT CGCCATTCAT GGTGGCTTAT GGGAACTTCC TGTTGGTCCT TCAGTATATC
    TGGTGTTTTG AGAAGTTGAA GCCGGTGTCT GGTTTCTTTG TGAAAATGGA GGTGCCTTTC
    ACTGAACTGA GCACCAAGGT TCTGTGTCAG CTGAGTTTCT GGCTACTGCT CAGACAAACT
    TTAATGGAGA GACAAGAAAG GAAGGAAGAA GAAGCAGCAC TTTCTGACAT CAAAGTAGAC
    GAGCAGAAAA AAGTGGAAGA AAAAAGTGAA GAGGAAGTGG ATGAGAACGA CCTGATGCAA
    GTGCTGGGCA AGCTGGTGAT GGCTCTGCTG GTCAAATACT GGATCTACGT CTGTGGAGGA
    ATGTTCTTCT TCGTCAGTTT TGAGGGAGAT ATGGCGACAT ATAAGATCAT CTACATGATG
    ATGCTGCTGT CCTGCGTCGC TCTTTACCAG GTACATTATG AATACTGGCG CAGGATCCTG
    AAGTATTTCT GGATGTCTGT GGTGGTTTAC ACTATGCTGG TGCTCATCCT GATCTACACC
    AGCCAGTTTG AGGATGGCAT CCCGACCTGG ACCAGGATGA CCGGCCTGGA CAAAGATGTA
    CTGAAGGATA TCGGTCTGGA GAAGTTCAGC CTCTCTAATT TGTTCACGCG TATCTTCATC
    CCGACGTCTT TCCTGTTGGT GTGTATCCTG CATCTGCACT ACTTCCATGA CCATTTTCTG
    GAGCTGACTG ATCTGAAAGC CGTGGTCAGC AAACAGGAGA GCACCATTTA CAGATTGGCT
    CATCCCGATG GGAGTTTGGC GGATCTGACC ATTGTGAGCT CGTCTCCGGA GCCGCTGCTG
    AAGGATGAGA AGGAAAAAGA GTCTCTGAAT GAGGTGCTGG TGGTGGATTG TGTGGGGGAA
    GAGAAGAAGA AAGACTCTTT ATCCATTTAT TCGGCTCAAA TGTCCAGTCA GGAGGACGTT
    CATCAGTCCA GTGCTGCCAC AGACCCTGAA CTGCCTTCAG AACAAAGCTC AGAGATGAAG
    AATAAGTGGC ATCTGGTGGT GGACCGGCTC ACAGTGCTGT TCCTCAAGTT CCTGGAGTAC
    TTTCATAAGC TGAAACTCTT TGTCTGGTGG CTGCTGGAGA TGCACATCAT CAAGATCGTG
    GCCACGTACA TCATCCTGCT CTCGGTCAAA GAGGTCTCGC TGCTGAACTT TGTGTTTTTG
    ATCTCCTGGG CGTTTGCGCT GCCGTATAAG CAGTTTCGAG TTCTCTCCTC CAGCGTCTGC
    ACCGTATGGA CGTGCGTTAT TATTGTCTGC AAGCTCTTTT ACCAGCTCAA AACCATCAAT
    CCTGCCAACT ACTCCAGTGA TTGTATTATG CCCAACTATA CAGAAACAGA AAAAAAGGAC
    ATGAAGACGT CTCTACTGTA TCGTGATCCA GTGGATCCTG CTAACTGGGT TGGCCTGCGG
    AAGTTTGATC ATGGGATAAT ATTGCCTAAC CTGAGGAATA ACTTGCTGAT GTTGGCCCTC
    TTGGCGTTCG AGGTTACCAT TTACAGACAC CAGGAGTTTT TCCGTCTAAG AAACAAACTT
    TCTCCTCCTC CTTCACGCAC CATCTTTCAT GACATCACCC GGCAACACCT TGACAACGGC
    ATCATCGAGT GCGCCAAGTA CTTTATTAAC TACTTCTTCT ACAAGTTTGG TTTAGAGGTG
    TGTTTTTTGT TGGCCATCAA TGTGATGGGT CAGCGGATGG ATTTCTACTC CATGGTCCAC
    GGCTTGGCTC TGGCTGTGGT GATGTTCAGA CGGCGGAGGA AAGCCATCGC TGAGATCTGG
    CCCAAATACT GCTGCTTCCT GGCCTGTATG ATCACCTTTC AGTATTTCAT ATGCATCGGG
    ATTCCACCGG CTGCTTGTGC AGATTATCCC TGGAGGTTTC CCAACTCCTC GATGACGTCT
    AATGTCATTA AATGGCTTTA CCTTCCTGAT TTCCACACTC CACCCAGCTC CATGTTCCTC
    GGCTATGATT TCATGCTGCT GCTGTGTGCG TCGCTGCAGA GGCAGGTGTT TGATGAAGAA
    AATAAAGCCG CCGTTCGCCT GATGGCGGGA GATAATGTGG AGATCTGCCG AGATCTGGAC
    GCTGCATCAT TCAGTGTTCA CAACCCGGTG CCAGACTTCA TTCACTGCAG GTCATACCTG
    GACATGCTGA AGGTGATCAT GTTCAGTTAC CTGTTCTGGT TCGTCCTGAC CATCATCTTC
    ATCACGGGCA CCACACGGAT CAGCATCTTC TGCATGGGTT ACCTGGTGGC CTGCTTTTAC
    TTCCTGCTGT TTGGTGGGCA ACTTTTACTC AAGCCAATCA AAGAGATCCT CCGTTACTGG
    GACTTTCTCA TCGCCTACAA CGTTTTCGTC ATCACCATGA AGAACGTCTT TGCAATTTTG
    GCGTGCGGTT ACATCAACTC GCTGGTGAAG AATAGCTGCT GGTTAATTCA GCTGCTCAGT
    CTGGCCTGCA CCATTAAAGA CTACCAACCA AAAACAGATC TGCCTGGAGT TGACTGTGAG
    CTGCCAAAAG ACGAGGCCGG GATCATCTGG GACAGCATCT GCTTCACCTT CCTCCTCCTG
    CAGAGAAGAG TCTTCATGAG CTACTATTTC TTGCATGTAA TCGCAGACAT TCGAGCCGGA
    CAGATTCTTG CATCCCGAGG AGCTGAGTTG TTTCAGGCCA GTATCGTGAA GGCTGTGAGA
    GCTCGTCTGG AGGAGGAGAA GAAATCCATG GAGCAACTGA AGAGACAGAT GGATCGTATT
    AAAACGAGAC AGCAGAAGTT CAAGAGAGGA AAAGAGAAGA TGCTCAGCAT AGCCCAAGAG
    TCTGGAGACG GAGAGAAACT CATCCCACCA GTGGACAATG AGGAGGAGGA GGACGAGGAG
    AAAAAGAAAA ACAAGGTGCA AAAAAAGCAG TGGTGGAGGC CTTGGGTTGA CCATGCGTCC
    ATGGTTAGAA GTGGTGATTA TTACCTGTTT GAAACTGACA GTGAAGAGGA GGAGGAAGAG
    GAGGAGAAAA AAGACGAAGA GCCTCCCAAA AGATCAGCCT TTCAGCGAGC GATCAGAAAG
    TTTGCTTCAG CCCTTGTTGC TTTACCCAGA TCAGTCATTA AACTGCCCAA AACTATTCTG
    CAGTATTTAA TAAGGGCAGC CAAGTTTGTG TATCATACCT GGATCGCCGA ATCTAAAGCA
    GCTCTGAAAG AAAGAGGAAA AGGCAAGCGC TTCTGGAAGC GATACGGTCG CCGGCTTAAA
    AAAGAGAAGA AAGAAGAACA TGTGGCGATA GAGATCGGTG AAGACGAGCG TCAGAGTTCA
    GAAGAGGAGA AGACAGATGG ACCAGACAAC ATCATCAAGA GGGTGTTCAA CATCATCAAG
    TTCACCTGGG CTCTGTTTCT GACCACCGTG GAGAGCATCA CCAAATGGCT GAACTCAGTC
    TGCAGAGAAT ATATCGACAT CTCCACCGTC CTGCGCATCG AGCGCTGCAT GCTGACCCGA
    GAAGTCAAAA AGGGGAATGT GCCGTCCCGT GAGAGCATCC ATGTGTACTA CCAGAAGCAG
    ATGAAGCTGA ATGGATCTCG TGAATCTGGT CTGGACCGCA TCAGTGAGGA GGATTCAGCC
    TCAAACAGAG AACGCCGCAG ACGCGCACAA CACAGTCTGG ATTCATTCGC TTCCAGAGAC
    AGCATCTCCA GCGCATACAC CGAAGCCACC ATGCTGTTTT CCCGCCAGTC CACCTTGGAC
    GATTTGGACG ACATGCCTCA GAATATTCCC AAAACCAGCG AACGCGCTCG ACCCAAACTG
    CGCAAAATGT ACGGCCTCGA CATGTCCAAC TCCTCTGCGG ACAGCGGAAG CAGCGTCATC
    TCCAGTGAGG CTACTCAGTG CGTCACGCTG TTTTCTCGGC AAGGAACCAA CGACACTATC
    GACGAGGCTG AAGACGAGCG AGAACAGCTG GAGCACAAGC AGAAAACAGA AGAAGCAGAA
    CTCAGAGACA AACCTGAAGA AGAGCAGGAA GTGGAAATAA GCGATCAGGG GAAACAGGAA
    GTGTTACCGG TGAGCCATCT GGTGACACAG GAAGTGAAAG AGGATGTACT ACCTGTGAGC
    GATCAGCTGA CTGGGGAAGT GACTGAGGAA GTTGAAGAGG AGGTGGAAGT AGTTAAGGAA
    ATGGAAGAGG AAGTAGAAAA GGAAATTGGA GCAAAGGTGG AAAAGGAAGT AGAAGCGGAA
    AAGGAAGAGG AAGCGGAAAA GGAAGTGGGT GAGGAAGTGC AATGGGACTT GGTGGATCCA
    GAGGAAGCAG AGGAGTGTGT AAGTGATGGG GACGGTGGTG AGCAGATGCA GCAGGATGAA
    GGTGAATGGT CGGGAGTGGA GAACGTGGTG AGCGGTCCGC TGTTCACACC AGACACTGAC
    GCCCCCAACA CGTCTGACGC TGACGTGCCG CCCAGCTACA GTAAAGCCGT GAGCTTCGAC
    CGGCTGTCTG TGAGCTCGGA CGACAGTGAC AGCGACAAAC GGCTGATGCT AATGACGCCT
    GACAGCAAAT CTGACCTGGA CGACCCTCTG CTGCCCTCCA TGACCACCGA CATGACCGCC
    AGCGAACTGC TGCTCAACAA AATGTTCTAC GACGAGATGC TGGACAGCTC AGATCGCTTC
    TATAAGTCTC AGCCGCTGGG TCTCCAGTTG TGTTACGCTC TCTACAACCT GCTGGTGGCT
    CACTCAGAAA TGGTGTGTTA TCTGGTCATC ATCCTCAACC ACATGATCTC CGCCTCCATG
    GCCACGCTGG TGCTGCCCAT CCTCATCTTC CTGTGGGCCA TGCTGTCCGT TCCGCGACCC
    AGCAAGAGGT TCTGGATGAC CGCCATCGTC TACACTGAGG TCACCATCGT CATCAAATAC
    TTCTTCCAGT TCAGCTTTTT CCCTTTCAAT CAGAACCTGG AGGTGAACAA GGGCAAGCCG
    TACCACCCGC CCAACATCCT GGGCACAGAG AAAAAAGACG GATACGTGCA CTACGACCTG
    GTCCAGCTGC TGGCGCTCTT CTTCCACAGA TCCATACTAA AGTGTCATGG TCTCTGGGAC
    GAGGACGACC CCAAAGTGCC CCGTAAGGAT GAGTGTCCTC GTCAGGAGTC TGTGGATGAC
    GGGAAGCTGT CGGTGGTCAT GTCACCTGAT GAAGACAGGA AGAGTTCTCC GGCGTATTCA
    CTCAAGTCTA TGAATCTGGG AATGTCTGTG GACTCATCGC AGGTTCACGT ACAGATGCGT
    TATCCAGAGC AGCGGCCGTA CCTGCGCAGA CAGAGCACCA GCGGATCACA CTTCTCCCAC
    AGATCCTCCG CACGCTCCAA ACGCGGCAGC ACAAGCACAC GTCACAGCAC CCATGGGGAA
    AACAGCACAA CAGAGTCAGA AGTACCACAA AAGAGCCGGA AAGAAATGAT TATGGAGAAA
    ATCAGAGAGC AGCTCATCAA AGCAAAGGTT TACCTGCTCA AAAGATTGGT TGAGTTTTAT
    CAGCCCATCC GTCAGTTCTT CTACAACCTG GTTCATCCGG AGTACAGCGC CGTGACGGAT
    GTTTACGTAT TGATGTTTCT AGCAGACACT GTAGATTTTA TCATCATCGT CTTTGGCTTT
    TGGGCATTTG GGAAACATCA GGGTGGTGCT GACATCACTT CCTCCCTGTC AGAGGACCAG
    GTTCCTGGGC CGTTCCTTGT TATGGTTCTC ATACAGTTTG GGACGATGGT AGTGGATCGA
    GCCCTGTATC TGCGCAAGAC TGTTGTGGGA AAAGTGATTT TTCAGGTCAT CCTGGTGTTT
    GGCATCCATT TCTGGATGTT TTTCATCCTG CCGGGAATCA CAGAGAGGCG CTTCAGTCAG
    AACACTATCG CTCAGCTCTG GTACTTTGTG AAGTGCATTT ACTTCGGCCT GTCAGCCTAT
    CAGATCAGAT GTGGATATCC AACCCGCATC CTGGGAAACT TCCTCACCAA GAGCTACAAC
    TACGCCAACC TCTTCCTCTT CCAGGGTTTC CGTCTGATCC CGTTCCTGAC GGAGCTGCGT
    GCGGTGATGG ACTGGGTCTG GACGGACACA ACGCTCAGCT TGTCCAGCTG GATCTGTGTG
    GAGGACATCT ATGCTCACAT CTTCATCCTC AAATGCTGGA GGGAGTCTGA GAAGCGTTAC
    CCTCAGCCGC GCGGGCAGAA GAAGAAGAAG GTGGTGAAGT ATGGGATGGG CGGCATGATC
    GTGATGCTCC TGATCTGCAT CGTCTGGTTT CCTCTGCTCT TCATGTCTCT GGTCAAATCT
    GTGGCAGGAG TGGTTAACAA GCCTCTGGAG GTGTCCGTCT CGCTCACGCT CGGAGGACTC
    CAGCCGATCT TCACAATGAG TGCCCAGCAG AACCATATCA AATCCGTCAC TGGGGCAGAA
    TTTAAGAAGT TCACAGATCA CTACAAGTCT AACGCAAATG CCATCATGTT CCTGGAGTCG
    TATTTTCCTG AGGATCTGAC AATAGCACAT CTAGAGGGAA GTTCAAACTC ACTCTGGACC
    ATCAGCCCCC CGAGCAAGGA AAACCTGAAG AATATGCTCA ATGACACGAG AGAGCCGTTT
    CCTCTCACCT TCACCTGGTC CATTCAAAGG AACCTCAGTT TAGGTGCCAA AGCTGAAACT
    GCAATGGGGA AATACTTCAT TGAGCTGGAC GACGAGACTA GAAAAGGTCT AACTGATCAA
    CTGGGGAATA GCAGCCAGCG TGTGACAATC AAAAAAATAT TTCCAAGGTA CATCAGAGCA
    CCAAGCGACT CCAATGCCAA ACCTATTGAC CAGCTTTACA ACGATGGAGA GTACATGGAC
    ATCACACTGT TTTTAAATAA GAAGAACAGC TCAGAAGGCG TTCAGGAGTG GTGGATCGTC
    AATCAGACAG ATTCAGGTCC ATTACCTGGA CTCAACGGGT CTGGTCTTCA GATCTACATC
    ATCAGTGACC AAGTCAGTCC ACCCAGTCTG GGCTTTCTGG CTGGTTATGG GATCATGGGT
    CTGTACATGT CAGTGGTTTT GGTGATCGGG AAGTTTGTGC GCGAGTTCTT CAGCGGGATC
    TCGCACACCA TCATGTTCGA AGAGCTCCCT AACGTGGACC GAATCCTCAA ACTCTGCACC
    GATATCTTCC TGGTCCGAGA GACGGGCGAA CTGGACCTGG AGGAAGACCT TTACGCCAAA
    CTCATCTTCC TCTACCGCTC TCCAGAAACC ATGATCAAAT GGACCAGAGA GAAGAGTGAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150521
    Clone ID Related Accession (Same CDS sequence) XM_021468277.1
    Accession Version XM_021468277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product piezo-type mechanosensitive ion channel component 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2641
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    4141
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    4441
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    7681
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    7921
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    8281
    8341
    8401
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    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGCTTCTG AAGTTGTGTG TGGTTTGGTC TTCAGAGTGC TGTTGCCGGT GTGTCTGGCG 
    GCAGCATGTC TGTTCAGGTT CAATGGGCTG TCCTTCATCT ACCTCCTCTT CCTCCTCCTC
    ATCCCGCTGT TCCCAGAGCC CTCCAGCATC ACCATGAGAG GCCAGACGGG GTACTTGCTG
    AAGTCTTTAT GCTGCTGCAG TGTGACCTTC CTCTTCTTCC ACATCATCTA TCAAATCACC
    GTCCACAGTT TGCTGGCAAG AGGAAGAGTC GATTCTGGAT TCAACTGTTC GGTGTGGGAG
    AAGTCAATAC GACAGATTGG TTTTGAGAGT GTTATCGGGG CAGATGCAGG CAATGGTATC
    CGAGTTTTCC TGCCAGACAT TGGGATGTTC ATGGCCAGTC TGGGTGTGTG GCTCCTGTGC
    CGAAAACTGC TCCACCAACG GCCCACTGAG GATATGGCCC AGGACAACCA GCAATTCCTG
    TCTGAGGAGA AGGAGGAGGA AAACGAGAAG CTGGAAAATG AAGAAGATGA GAATGATAAT
    AATGATGATG ATGATGATGA AATGCTGTTT GACGAGGACT TTGATGCAGA AGATGAAGCA
    GAGGAAGAAG ATGGAGAAGC CGGAAGACTG GAGGAGGAGG AGGAGGAAGA GGAGGAGGAA
    GAGGAAGTGC AGGAGAGCAC CAAGATGAAG ATCCTGCGCG CGGTTGGAGG AGTCGCTGCC
    AAACTCAAGG AGATCATCGG GAACCTCATC ACTACTGCTG GACAGGTGGT GGTCACTATC
    ATGCTGGGAA TGACAGGTGT GACGCTGCCT TCTCTGTCCT CCGCTGTGTA TTTCTTCGTG
    TTTCTGGGTC TGTGCACATG GTGGTCTCTC TGTAAGACCT TCGATAAGCT CTTGTTCAGC
    TGTCTGTGTG TCCTGATGGC CATTTTCAGC GCTGGACATC TCATCGTGCT TTATATCAAC
    CAGTTTCAGT TCCTCCAGGA AGCCATAAGT CCCACCGATG GATATACCAG TGTTTTTGGC
    ATCTCCTCCA TCATTTACAC AGACTGCTCC TCTACATGGA AGCTTGCTGT CAGGAGTGGA
    CTCAAATGGC ATCATTTCGT CAATCCCATA ATGATCCTGG TCCTGTATTA CACACTGGCC
    ACGCTGATCC GCCTGTGGCT GCAGGATCCT ATGATGATGG AGGACAGCGA GGACGGAGAG
    GTGATCGAGG GCAATGAAGC CATGGAAAAC AACGTCCATC ACACGTCCAT CCGGAAACGG
    CTGCTCTGGT GGAAAGCGCA TCAGAAAACC GAGGAGAGAA ATCTGATCTC CACTCAGGAT
    GGGTACAGCA CCTCGGAGGT GTTAGTGGTC ACCTCCAATG GGACGTCGTT TGATTATGTG
    TCCGCCGTGC CCATCGAGAA CGGACCTGTC AGTCTGGACA TTTACTCCAC TCCTCAGTAC
    AAAGTGGATC AGTCTGTCGA ATATGACGAG AAGACGGATG AGAACACAAA TGAGGTGCTG
    GACTCACAGC TGGAGGAGGA AGAGGAGAAG ACAGATGAGG AAGAGGAAGC CGAAGAGGAA
    GAGCAGGGCG GACCACCGGC AGCTCTCGTC AAAGTGTTCA AGTTCATCAT GAAGCAGAGC
    TACATCTGCG CTCTCATCGC TATGATGGCG TGGAGTATAA CGTATGTCAG CTGGCTGACG
    TTTGTCTTTC TGATCTGGTC CTGTACTCTG TGGATGGTGC GCGACCGGAG GAAATACGCC
    ATGCTGACTT CGCCATTCAT GGTGGCTTAT GGGAACTTCC TGTTGGTCCT TCAGTATATC
    TGGTGTTTTG AGAAGTTGAA GCCGGTGTCT GGTTTCTTTG TGAAAATGGA GGTGCCTTTC
    ACTGAACTGA GCACCAAGGT TCTGTGTCAG CTGAGTTTCT GGCTACTGCT CAGACAAACT
    TTAATGGAGA GACAAGAAAG GAAGGAAGAA GAAGCAGCAC TTTCTGACAT CAAAGTAGAC
    GAGCAGAAAA AAGTGGAAGA AAAAAGTGAA GAGGAAGTGG ATGAGAACGA CCTGATGCAA
    GTGCTGGGCA AGCTGGTGAT GGCTCTGCTG GTCAAATACT GGATCTACGT CTGTGGAGGA
    ATGTTCTTCT TCGTCAGTTT TGAGGGAGAT ATGGCGACAT ATAAGATCAT CTACATGATG
    ATGCTGCTGT CCTGCGTCGC TCTTTACCAG GTACATTATG AATACTGGCG CAGGATCCTG
    AAGTATTTCT GGATGTCTGT GGTGGTTTAC ACTATGCTGG TGCTCATCCT GATCTACACC
    AGCCAGTTTG AGGATGGCAT CCCGACCTGG ACCAGGATGA CCGGCCTGGA CAAAGATGTA
    CTGAAGGATA TCGGTCTGGA GAAGTTCAGC CTCTCTAATT TGTTCACGCG TATCTTCATC
    CCGACGTCTT TCCTGTTGGT GTGTATCCTG CATCTGCACT ACTTCCATGA CCATTTTCTG
    GAGCTGACTG ATCTGAAAGC CGTGGTCAGC AAACAGGAGA GCACCATTTA CAGCTATGCT
    AAAGTCAGTG GTCGTATCTA TCTGATCGTG GATAGCATCA AGCAAAAAAT ATCTTCTTCT
    CAGAGCAAAT TGGCTCATCC CGATGGGAGT TTGGCGGATC TGACCATTGT GAGCTCGTCT
    CCGGAGCCGC TGCTGAAGGA TGAGAAGGAA AAAGAGTCTC TGAATGAGGT GCTGGTGGTG
    GATTGTGTGG GGGAAGAGAA GAAGAAAGAC TCTTTATCCA TTTATTCGGC TCAAATGTCC
    AGTCAGGAGG ACGTTCATCA GTCCAGTGCT GCCACAGACC CTGAACTGCC TTCAGAACAA
    AGCTCAGAGA TGAAGAATAA GTGGCATCTG GTGGTGGACC GGCTCACAGT GCTGTTCCTC
    AAGTTCCTGG AGTACTTTCA TAAGCTGAAA CTCTTTGTCT GGTGGCTGCT GGAGATGCAC
    ATCATCAAGA TCGTGGCCAC GTACATCATC CTGCTCTCGG TCAAAGAGGT CTCGCTGCTG
    AACTTTGTGT TTTTGATCTC CTGGGCGTTT GCGCTGCCGT ATAAGCAGTT TCGAGTTCTC
    TCCTCCAGCG TCTGCACCGT ATGGACGTGC GTTATTATTG TCTGCAAGCT CTTTTACCAG
    CTCAAAACCA TCAATCCTGC CAACTACTCC AGTGATTGTA TTATGCCCAA CTATACAGAA
    ACAGAAAAAA AGGACATGAA GACGTCTCTA CTGTATCGTG ATCCAGTGGA TCCTGCTAAC
    TGGGTTGGCC TGCGGAAGTT TGATCATGGG ATAATATTGC CTAACCTGAG GAATAACTTG
    CTGATGTTGG CCCTCTTGGC GTTCGAGGTT ACCATTTACA GACACCAGGA GTTTTTCCGT
    CTAAGAAACA AACTTTCTCC TCCTCCTTCA CGCACCATCT TTCATGACAT CACCCGGCAA
    CACCTTGACA ACGGCATCAT CGAGTGCGCC AAGTACTTTA TTAACTACTT CTTCTACAAG
    TTTGGTTTAG AGGTGTGTTT TTTGTTGGCC ATCAATGTGA TGGGTCAGCG GATGGATTTC
    TACTCCATGG TCCACGGCTT GGCTCTGGCT GTGGTGATGT TCAGACGGCG GAGGAAAGCC
    ATCGCTGAGA TCTGGCCCAA ATACTGCTGC TTCCTGGCCT GTATGATCAC CTTTCAGTAT
    TTCATATGCA TCGGGATTCC ACCGGCTGCT TGTGCAGATT ATCCCTGGAG GTTTCCCAAC
    TCCTCGATGA CGTCTAATGT CATTAAATGG CTTTACCTTC CTGATTTCCA CACTCCACCC
    AGCTCCATGT TCCTCGGCTA TGATTTCATG CTGCTGCTGT GTGCGTCGCT GCAGAGGCAG
    GTGTTTGATG AAGAAAATAA AGCCGCCGTT CGCCTGATGG CGGGAGATAA TGTGGAGATC
    TGCCGAGATC TGGACGCTGC ATCATTCAGT GTTCACAACC CGGTGCCAGA CTTCATTCAC
    TGCAGGTCAT ACCTGGACAT GCTGAAGGTG ATCATGTTCA GTTACCTGTT CTGGTTCGTC
    CTGACCATCA TCTTCATCAC GGGCACCACA CGGATCAGCA TCTTCTGCAT GGGTTACCTG
    GTGGCCTGCT TTTACTTCCT GCTGTTTGGT GGGCAACTTT TACTCAAGCC AATCAAAGAG
    ATCCTCCGTT ACTGGGACTT TCTCATCGCC TACAACGTTT TCGTCATCAC CATGAAGAAC
    GTCTTTGCAA TTTTGGCGTG CGGTTACATC AACTCGCTGG TGAAGAATAG CTGCTGGTTA
    ATTCAGCTGC TCAGTCTGGC CTGCACCATT AAAGACTACC AACCAAAAAC AGATCTGCCT
    GGAGTTGACT GTGAGCTGCC AAAAGACGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    ACCTTCCTCC TCCTGCAGAG AAGAGTCTTC ATGAGCTACT ATTTCTTGCA TGTAATCGCA
    GACATTCGAG CCGGACAGAT TCTTGCATCC CGAGGAGCTG AGTTGTTTCA GGCCAGTATC
    GTGAAGGCTG TGAGAGCTCG TCTGGAGGAG GAGAAGAAAT CCATGGAGCA ACTGAAGAGA
    CAGATGGATC GTATTAAAAC GAGACAGCAG AAGTTCAAGA GAGGAAAAGA GAAGATGCTC
    AGCATAGCCC AAGAGTCTGG AGACGGAGAG AAACTCATCC CACCAGTGGA CAATGAGGAG
    GAGGAGGACG AGGAGAAAAA GAAAAACAAG GTGCAAAAAA AGCAGTGGTG GAGGCCTTGG
    GTTGACCATG CGTCCATGGT TAGAAGTGGT GATTATTACC TGTTTGAAAC TGACAGTGAA
    GAGGAGGAGG AAGAGGAGGA GAAAAAAGAC GAAGAGCCTC CCAAAAGATC AGCCTTTCAG
    TTTGTGTATC ATACCTGGAT CGCCGAATCT AAAGCAGCTC TGAAAGAAAG AGGAAAAGGC
    AAGCGCTTCT GGAAGCGATA CGGTCGCCGG CTTAAAAAAG AGAAGAAAGA AGAACATGTG
    GCGATAGAGA TCGGTGAAGA CGAGCGTCAG AGTTCAGAAG AGGAGAAGAC AGATGGACCA
    GACAACATCA TCAAGAGGGT GTTCAACATC ATCAAGTTCA CCTGGGCTCT GTTTCTGACC
    ACCGTGGAGA GCATCACCAA ATGGCTGAAC TCAGTCTGCA GAGAATATAT CGACATCTCC
    ACCGTCCTGC GCATCGAGCG CTGCATGCTG ACCCGAGAAG TCAAAAAGGG GAATGTGCCG
    TCCCGTGAGA GCATCCATGT GTACTACCAG AAGCAGATGA AGCTGAATGG ATCTCGTGAA
    TCTGGTCTGG ACCGCATCAG TGAGGAGGAT TCAGCCTCAA ACAGAGAACG CCGCAGACGC
    GCACAACACA GTCTGGATTC ATTCGCTTCC AGAGACAGCA TCTCCAGCGC ATACACCGAA
    GCCACCATGC TGTTTTCCCG CCAGTCCACC TTGGACGATT TGGACGACAT GCCTCAGAAT
    ATTCCCAAAA CCAGCGAACG CGCTCGACCC AAACTGCGCA AAATGTACGG CCTCGACATG
    TCCAACTCCT CTGCGGACAG CGGAAGCAGC GTCATCTCCA GTGAGGCTAC TCAGTGCGTC
    ACGCTGTTTT CTCGGCAAGG AACCAACGAC ACTATCGACG AGGCTGAAGA CGAGCGAGAA
    CAGCTGGAGC ACAAGCAGAA AACAGAAGAA GCAGAACTCA GAGACAAACC TGAAGAAGAG
    CAGGAAGTGG AAATAAGCGA TCAGGGGAAA CAGGAAGTGT TACCGGTGAG CCATCTGGTG
    ACACAGGAAG TGAAAGAGGA TGTACTACCT GTGAGCGATC AGCTGACTGG GGAAGTGACT
    GAGGAAGTTG AAGAGGAGGT GGAAGTAGTT AAGGAAATGG AAGAGGAAGT AGAAAAGGAA
    ATTGGAGCAA AGGTGGAAAA GGAAGTAGAA GCGGAAAAGG AAGAGGAAGC GGAAAAGGAA
    GTGGGTGAGG AAGTGCAATG GGACTTGGTG GATCCAGAGG AAGCAGAGGA GTGTGTAAGT
    GATGGGGACG GTGGTGAGCA GATGCAGCAG GATGAAGGTG AATGGTCGGG AGTGGAGAAC
    GTGGTGAGCG GTCCGCTGTT CACACCAGAC ACTGACGCCC CCAACACGTC TGACGCTGAC
    GTGCCGCCCA GCTACAGTAA AGCCGTGAGC TTCGACCGGC TGTCTGTGAG CTCGGACGAC
    AGTGACAGCG ACAAACGGCT GATGCTAATG ACGCCTGACA GCAAATCTGA CCTGGACGAC
    CCTCTGCTGC CCTCCATGAC CACCGACATG ACCGCCAGCG AACTGCTGCT CAACAAAATG
    TTCTACGACG AGATGCTGGA CAGCTCAGAT CGCTTCTATA AGTCTCAGCC GCTGGGTCTC
    CAGTTGTGTT ACGCTCTCTA CAACCTGCTG GTGGCTCACT CAGAAATGGT GTGTTATCTG
    GTCATCATCC TCAACCACAT GATCTCCGCC TCCATGGCCA CGCTGGTGCT GCCCATCCTC
    ATCTTCCTGT GGGCCATGCT GTCCGTTCCG CGACCCAGCA AGAGGTTCTG GATGACCGCC
    ATCGTCTACA CTGAGGTCAC CATCGTCATC AAATACTTCT TCCAGTTCAG CTTTTTCCCT
    TTCAATCAGA ACCTGGAGGT GAACAAGGGC AAGCCGTACC ACCCGCCCAA CATCCTGGGC
    ACAGAGAAAA AAGACGGATA CGTGCACTAC GACCTGGTCC AGCTGCTGGC GCTCTTCTTC
    CACAGATCCA TACTAAAGTG TCATGGTCTC TGGGACGAGG ACGACCCCAA AGTGCCCCGT
    AAGGATGAGT GTCCTCGTCA GGAGTCTGTG GATGACGGGA AGCTGTCGGT GGTCATGTCA
    CCTGATGAAG ACAGGAAGAG TTCTCCGGCG TATTCACTCA AGTCTATGAA TCTGGGAATG
    TCTGTGGACT CATCGCAGGT TCACGTACAG ATGCGTTATC CAGAGCAGCG GCCGTACCTG
    CGCAGACAGA GCACCAGCGG ATCACACTTC TCCCACAGAT CCTCCGCACG CTCCAAACGC
    GGCAGCACAA GCACACGTCA CAGCACCCAT GGGGAAAACA GCACAACAGA GTCAGAAGTA
    CCACAAAAGA GCCGGAAAGA AATGATTATG GAGAAAATCA GAGAGCAGCT CATCAAAGCA
    AAGGTTTACC TGCTCAAAAG ATTGGTTGAG TTTTATCAGC CCATCCGTCA GTTCTTCTAC
    AACCTGGTTC ATCCGGAGTA CAGCGCCGTG ACGGATGTTT ACGTATTGAT GTTTCTAGCA
    GACACTGTAG ATTTTATCAT CATCGTCTTT GGCTTTTGGG CATTTGGGAA ACATCAGGGT
    GGTGCTGACA TCACTTCCTC CCTGTCAGAG GACCAGGTTC CTGGGCCGTT CCTTGTTATG
    GTTCTCATAC AGTTTGGGAC GATGGTAGTG GATCGAGCCC TGTATCTGCG CAAGACTGTT
    GTGGGAAAAG TGATTTTTCA GGTCATCCTG GTGTTTGGCA TCCATTTCTG GATGTTTTTC
    ATCCTGCCGG GAATCACAGA GAGGCGCTTC AGTCAGAACA CTATCGCTCA GCTCTGGTAC
    TTTGTGAAGT GCATTTACTT CGGCCTGTCA GCCTATCAGA TCAGATGTGG ATATCCAACC
    CGCATCCTGG GAAACTTCCT CACCAAGAGC TACAACTACG CCAACCTCTT CCTCTTCCAG
    GGTTTCCGTC TGATCCCGTT CCTGACGGAG CTGCGTGCGG TGATGGACTG GGTCTGGACG
    GACACAACGC TCAGCTTGTC CAGCTGGATC TGTGTGGAGG ACATCTATGC TCACATCTTC
    ATCCTCAAAT GCTGGAGGGA GTCTGAGAAG CGTTACCCTC AGCCGCGCGG GCAGAAGAAG
    AAGAAGGTGG TGAAGTATGG GATGGGCGGC ATGATCGTGA TGCTCCTGAT CTGCATCGTC
    TGGTTTCCTC TGCTCTTCAT GTCTCTGGTC AAATCTGTGG CAGGAGTGGT TAACAAGCCT
    CTGGAGGTGT CCGTCTCGCT CACGCTCGGA GGACTCCAGC CGATCTTCAC AATGAGTGCC
    CAGCAGAACC ATATCAAATC CGTCACTGGG GCAGAATTTA AGAAGTTCAC AGATCACTAC
    AAGTCTAACG CAAATGCCAT CATGTTCCTG GAGTCGTATT TTCCTGAGGA TCTGACAATA
    GCACATCTAG AGGGAAGTTC AAACTCACTC TGGACCATCA GCCCCCCGAG CAAGGAAAAC
    CTGAAGAATA TGCTCAATGA CACGAGAGAG CCGTTTCCTC TCACCTTCAC CTGGTCCATT
    CAAAGGAACC TCAGTTTAGG TGCCAAAGCT GAAACTGCAA TGGGGAAATA CTTCATTGAG
    CTGGACGACG AGACTAGAAA AGGTCTAACT GATCAACTGG GGAATAGCAG CCAGCGTGTG
    ACAATCAAAA AAATATTTCC AAGGTACATC AGAGCACCAA GCGACTCCAA TGCCAAACCT
    ATTGACCAGC TTTACAACGA TGGAGAGTAC ATGGACATCA CACTGTTTTT AAATAAGAAG
    AACAGCTCAG AAGGCGTTCA GGAGTGGTGG ATCGTCAATC AGACAGATTC AGGTCCATTA
    CCTGGACTCA ACGGGTCTGG TCTTCAGATC TACATCATCA GTGACCAAGT CAGTCCACCC
    AGTCTGGGCT TTCTGGCTGG TTATGGGATC ATGGGTCTGT ACATGTCAGT GGTTTTGGTG
    ATCGGGAAGT TTGTGCGCGA GTTCTTCAGC GGGATCTCGC ACACCATCAT GTTCGAAGAG
    CTCCCTAACG TGGACCGAAT CCTCAAACTC TGCACCGATA TCTTCCTGGT CCGAGAGACG
    GGCGAACTGG ACCTGGAGGA AGACCTTTAC GCCAAACTCA TCTTCCTCTA CCGCTCTCCA
    GAAACCATGA TCAAATGGAC CAGAGAGAAG AGTGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323952.1
    CDS675..9593
    Translation

    Target ORF information:

    RefSeq Version XM_021468277.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio piezo-type mechanosensitive ion channel component 2 (LOC100534973), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGCTTCTG AAGTTGTGTG TGGTTTGGTC TTCAGAGTGC TGTTGCCGGT GTGTCTGGCG 
    GCAGCATGTC TGTTCAGGTT CAATGGGCTG TCCTTCATCT ACCTCCTCTT CCTCCTCCTC
    ATCCCGCTGT TCCCAGAGCC CTCCAGCATC ACCATGAGAG GCCAGACGGG GTACTTGCTG
    AAGTCTTTAT GCTGCTGCAG TGTGACCTTC CTCTTCTTCC ACATCATCTA TCAAATCACC
    GTCCACAGTT TGCTGGCAAG AGGAAGAGTC GATTCTGGAT TCAACTGTTC GGTGTGGGAG
    AAGTCAATAC GACAGATTGG TTTTGAGAGT GTTATCGGGG CAGATGCAGG CAATGGTATC
    CGAGTTTTCC TGCCAGACAT TGGGATGTTC ATGGCCAGTC TGGGTGTGTG GCTCCTGTGC
    CGAAAACTGC TCCACCAACG GCCCACTGAG GATATGGCCC AGGACAACCA GCAATTCCTG
    TCTGAGGAGA AGGAGGAGGA AAACGAGAAG CTGGAAAATG AAGAAGATGA GAATGATAAT
    AATGATGATG ATGATGATGA AATGCTGTTT GACGAGGACT TTGATGCAGA AGATGAAGCA
    GAGGAAGAAG ATGGAGAAGC CGGAAGACTG GAGGAGGAGG AGGAGGAAGA GGAGGAGGAA
    GAGGAAGTGC AGGAGAGCAC CAAGATGAAG ATCCTGCGCG CGGTTGGAGG AGTCGCTGCC
    AAACTCAAGG AGATCATCGG GAACCTCATC ACTACTGCTG GACAGGTGGT GGTCACTATC
    ATGCTGGGAA TGACAGGTGT GACGCTGCCT TCTCTGTCCT CCGCTGTGTA TTTCTTCGTG
    TTTCTGGGTC TGTGCACATG GTGGTCTCTC TGTAAGACCT TCGATAAGCT CTTGTTCAGC
    TGTCTGTGTG TCCTGATGGC CATTTTCAGC GCTGGACATC TCATCGTGCT TTATATCAAC
    CAGTTTCAGT TCCTCCAGGA AGCCATAAGT CCCACCGATG GATATACCAG TGTTTTTGGC
    ATCTCCTCCA TCATTTACAC AGACTGCTCC TCTACATGGA AGCTTGCTGT CAGGAGTGGA
    CTCAAATGGC ATCATTTCGT CAATCCCATA ATGATCCTGG TCCTGTATTA CACACTGGCC
    ACGCTGATCC GCCTGTGGCT GCAGGATCCT ATGATGATGG AGGACAGCGA GGACGGAGAG
    GTGATCGAGG GCAATGAAGC CATGGAAAAC AACGTCCATC ACACGTCCAT CCGGAAACGG
    CTGCTCTGGT GGAAAGCGCA TCAGAAAACC GAGGAGAGAA ATCTGATCTC CACTCAGGAT
    GGGTACAGCA CCTCGGAGGT GTTAGTGGTC ACCTCCAATG GGACGTCGTT TGATTATGTG
    TCCGCCGTGC CCATCGAGAA CGGACCTGTC AGTCTGGACA TTTACTCCAC TCCTCAGTAC
    AAAGTGGATC AGTCTGTCGA ATATGACGAG AAGACGGATG AGAACACAAA TGAGGTGCTG
    GACTCACAGC TGGAGGAGGA AGAGGAGAAG ACAGATGAGG AAGAGGAAGC CGAAGAGGAA
    GAGCAGGGCG GACCACCGGC AGCTCTCGTC AAAGTGTTCA AGTTCATCAT GAAGCAGAGC
    TACATCTGCG CTCTCATCGC TATGATGGCG TGGAGTATAA CGTATGTCAG CTGGCTGACG
    TTTGTCTTTC TGATCTGGTC CTGTACTCTG TGGATGGTGC GCGACCGGAG GAAATACGCC
    ATGCTGACTT CGCCATTCAT GGTGGCTTAT GGGAACTTCC TGTTGGTCCT TCAGTATATC
    TGGTGTTTTG AGAAGTTGAA GCCGGTGTCT GGTTTCTTTG TGAAAATGGA GGTGCCTTTC
    ACTGAACTGA GCACCAAGGT TCTGTGTCAG CTGAGTTTCT GGCTACTGCT CAGACAAACT
    TTAATGGAGA GACAAGAAAG GAAGGAAGAA GAAGCAGCAC TTTCTGACAT CAAAGTAGAC
    GAGCAGAAAA AAGTGGAAGA AAAAAGTGAA GAGGAAGTGG ATGAGAACGA CCTGATGCAA
    GTGCTGGGCA AGCTGGTGAT GGCTCTGCTG GTCAAATACT GGATCTACGT CTGTGGAGGA
    ATGTTCTTCT TCGTCAGTTT TGAGGGAGAT ATGGCGACAT ATAAGATCAT CTACATGATG
    ATGCTGCTGT CCTGCGTCGC TCTTTACCAG GTACATTATG AATACTGGCG CAGGATCCTG
    AAGTATTTCT GGATGTCTGT GGTGGTTTAC ACTATGCTGG TGCTCATCCT GATCTACACC
    AGCCAGTTTG AGGATGGCAT CCCGACCTGG ACCAGGATGA CCGGCCTGGA CAAAGATGTA
    CTGAAGGATA TCGGTCTGGA GAAGTTCAGC CTCTCTAATT TGTTCACGCG TATCTTCATC
    CCGACGTCTT TCCTGTTGGT GTGTATCCTG CATCTGCACT ACTTCCATGA CCATTTTCTG
    GAGCTGACTG ATCTGAAAGC CGTGGTCAGC AAACAGGAGA GCACCATTTA CAGCTATGCT
    AAAGTCAGTG GTCGTATCTA TCTGATCGTG GATAGCATCA AGCAAAAAAT ATCTTCTTCT
    CAGAGCAAAT TGGCTCATCC CGATGGGAGT TTGGCGGATC TGACCATTGT GAGCTCGTCT
    CCGGAGCCGC TGCTGAAGGA TGAGAAGGAA AAAGAGTCTC TGAATGAGGT GCTGGTGGTG
    GATTGTGTGG GGGAAGAGAA GAAGAAAGAC TCTTTATCCA TTTATTCGGC TCAAATGTCC
    AGTCAGGAGG ACGTTCATCA GTCCAGTGCT GCCACAGACC CTGAACTGCC TTCAGAACAA
    AGCTCAGAGA TGAAGAATAA GTGGCATCTG GTGGTGGACC GGCTCACAGT GCTGTTCCTC
    AAGTTCCTGG AGTACTTTCA TAAGCTGAAA CTCTTTGTCT GGTGGCTGCT GGAGATGCAC
    ATCATCAAGA TCGTGGCCAC GTACATCATC CTGCTCTCGG TCAAAGAGGT CTCGCTGCTG
    AACTTTGTGT TTTTGATCTC CTGGGCGTTT GCGCTGCCGT ATAAGCAGTT TCGAGTTCTC
    TCCTCCAGCG TCTGCACCGT ATGGACGTGC GTTATTATTG TCTGCAAGCT CTTTTACCAG
    CTCAAAACCA TCAATCCTGC CAACTACTCC AGTGATTGTA TTATGCCCAA CTATACAGAA
    ACAGAAAAAA AGGACATGAA GACGTCTCTA CTGTATCGTG ATCCAGTGGA TCCTGCTAAC
    TGGGTTGGCC TGCGGAAGTT TGATCATGGG ATAATATTGC CTAACCTGAG GAATAACTTG
    CTGATGTTGG CCCTCTTGGC GTTCGAGGTT ACCATTTACA GACACCAGGA GTTTTTCCGT
    CTAAGAAACA AACTTTCTCC TCCTCCTTCA CGCACCATCT TTCATGACAT CACCCGGCAA
    CACCTTGACA ACGGCATCAT CGAGTGCGCC AAGTACTTTA TTAACTACTT CTTCTACAAG
    TTTGGTTTAG AGGTGTGTTT TTTGTTGGCC ATCAATGTGA TGGGTCAGCG GATGGATTTC
    TACTCCATGG TCCACGGCTT GGCTCTGGCT GTGGTGATGT TCAGACGGCG GAGGAAAGCC
    ATCGCTGAGA TCTGGCCCAA ATACTGCTGC TTCCTGGCCT GTATGATCAC CTTTCAGTAT
    TTCATATGCA TCGGGATTCC ACCGGCTGCT TGTGCAGATT ATCCCTGGAG GTTTCCCAAC
    TCCTCGATGA CGTCTAATGT CATTAAATGG CTTTACCTTC CTGATTTCCA CACTCCACCC
    AGCTCCATGT TCCTCGGCTA TGATTTCATG CTGCTGCTGT GTGCGTCGCT GCAGAGGCAG
    GTGTTTGATG AAGAAAATAA AGCCGCCGTT CGCCTGATGG CGGGAGATAA TGTGGAGATC
    TGCCGAGATC TGGACGCTGC ATCATTCAGT GTTCACAACC CGGTGCCAGA CTTCATTCAC
    TGCAGGTCAT ACCTGGACAT GCTGAAGGTG ATCATGTTCA GTTACCTGTT CTGGTTCGTC
    CTGACCATCA TCTTCATCAC GGGCACCACA CGGATCAGCA TCTTCTGCAT GGGTTACCTG
    GTGGCCTGCT TTTACTTCCT GCTGTTTGGT GGGCAACTTT TACTCAAGCC AATCAAAGAG
    ATCCTCCGTT ACTGGGACTT TCTCATCGCC TACAACGTTT TCGTCATCAC CATGAAGAAC
    GTCTTTGCAA TTTTGGCGTG CGGTTACATC AACTCGCTGG TGAAGAATAG CTGCTGGTTA
    ATTCAGCTGC TCAGTCTGGC CTGCACCATT AAAGACTACC AACCAAAAAC AGATCTGCCT
    GGAGTTGACT GTGAGCTGCC AAAAGACGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    ACCTTCCTCC TCCTGCAGAG AAGAGTCTTC ATGAGCTACT ATTTCTTGCA TGTAATCGCA
    GACATTCGAG CCGGACAGAT TCTTGCATCC CGAGGAGCTG AGTTGTTTCA GGCCAGTATC
    GTGAAGGCTG TGAGAGCTCG TCTGGAGGAG GAGAAGAAAT CCATGGAGCA ACTGAAGAGA
    CAGATGGATC GTATTAAAAC GAGACAGCAG AAGTTCAAGA GAGGAAAAGA GAAGATGCTC
    AGCATAGCCC AAGAGTCTGG AGACGGAGAG AAACTCATCC CACCAGTGGA CAATGAGGAG
    GAGGAGGACG AGGAGAAAAA GAAAAACAAG GTGCAAAAAA AGCAGTGGTG GAGGCCTTGG
    GTTGACCATG CGTCCATGGT TAGAAGTGGT GATTATTACC TGTTTGAAAC TGACAGTGAA
    GAGGAGGAGG AAGAGGAGGA GAAAAAAGAC GAAGAGCCTC CCAAAAGATC AGCCTTTCAG
    TTTGTGTATC ATACCTGGAT CGCCGAATCT AAAGCAGCTC TGAAAGAAAG AGGAAAAGGC
    AAGCGCTTCT GGAAGCGATA CGGTCGCCGG CTTAAAAAAG AGAAGAAAGA AGAACATGTG
    GCGATAGAGA TCGGTGAAGA CGAGCGTCAG AGTTCAGAAG AGGAGAAGAC AGATGGACCA
    GACAACATCA TCAAGAGGGT GTTCAACATC ATCAAGTTCA CCTGGGCTCT GTTTCTGACC
    ACCGTGGAGA GCATCACCAA ATGGCTGAAC TCAGTCTGCA GAGAATATAT CGACATCTCC
    ACCGTCCTGC GCATCGAGCG CTGCATGCTG ACCCGAGAAG TCAAAAAGGG GAATGTGCCG
    TCCCGTGAGA GCATCCATGT GTACTACCAG AAGCAGATGA AGCTGAATGG ATCTCGTGAA
    TCTGGTCTGG ACCGCATCAG TGAGGAGGAT TCAGCCTCAA ACAGAGAACG CCGCAGACGC
    GCACAACACA GTCTGGATTC ATTCGCTTCC AGAGACAGCA TCTCCAGCGC ATACACCGAA
    GCCACCATGC TGTTTTCCCG CCAGTCCACC TTGGACGATT TGGACGACAT GCCTCAGAAT
    ATTCCCAAAA CCAGCGAACG CGCTCGACCC AAACTGCGCA AAATGTACGG CCTCGACATG
    TCCAACTCCT CTGCGGACAG CGGAAGCAGC GTCATCTCCA GTGAGGCTAC TCAGTGCGTC
    ACGCTGTTTT CTCGGCAAGG AACCAACGAC ACTATCGACG AGGCTGAAGA CGAGCGAGAA
    CAGCTGGAGC ACAAGCAGAA AACAGAAGAA GCAGAACTCA GAGACAAACC TGAAGAAGAG
    CAGGAAGTGG AAATAAGCGA TCAGGGGAAA CAGGAAGTGT TACCGGTGAG CCATCTGGTG
    ACACAGGAAG TGAAAGAGGA TGTACTACCT GTGAGCGATC AGCTGACTGG GGAAGTGACT
    GAGGAAGTTG AAGAGGAGGT GGAAGTAGTT AAGGAAATGG AAGAGGAAGT AGAAAAGGAA
    ATTGGAGCAA AGGTGGAAAA GGAAGTAGAA GCGGAAAAGG AAGAGGAAGC GGAAAAGGAA
    GTGGGTGAGG AAGTGCAATG GGACTTGGTG GATCCAGAGG AAGCAGAGGA GTGTGTAAGT
    GATGGGGACG GTGGTGAGCA GATGCAGCAG GATGAAGGTG AATGGTCGGG AGTGGAGAAC
    GTGGTGAGCG GTCCGCTGTT CACACCAGAC ACTGACGCCC CCAACACGTC TGACGCTGAC
    GTGCCGCCCA GCTACAGTAA AGCCGTGAGC TTCGACCGGC TGTCTGTGAG CTCGGACGAC
    AGTGACAGCG ACAAACGGCT GATGCTAATG ACGCCTGACA GCAAATCTGA CCTGGACGAC
    CCTCTGCTGC CCTCCATGAC CACCGACATG ACCGCCAGCG AACTGCTGCT CAACAAAATG
    TTCTACGACG AGATGCTGGA CAGCTCAGAT CGCTTCTATA AGTCTCAGCC GCTGGGTCTC
    CAGTTGTGTT ACGCTCTCTA CAACCTGCTG GTGGCTCACT CAGAAATGGT GTGTTATCTG
    GTCATCATCC TCAACCACAT GATCTCCGCC TCCATGGCCA CGCTGGTGCT GCCCATCCTC
    ATCTTCCTGT GGGCCATGCT GTCCGTTCCG CGACCCAGCA AGAGGTTCTG GATGACCGCC
    ATCGTCTACA CTGAGGTCAC CATCGTCATC AAATACTTCT TCCAGTTCAG CTTTTTCCCT
    TTCAATCAGA ACCTGGAGGT GAACAAGGGC AAGCCGTACC ACCCGCCCAA CATCCTGGGC
    ACAGAGAAAA AAGACGGATA CGTGCACTAC GACCTGGTCC AGCTGCTGGC GCTCTTCTTC
    CACAGATCCA TACTAAAGTG TCATGGTCTC TGGGACGAGG ACGACCCCAA AGTGCCCCGT
    AAGGATGAGT GTCCTCGTCA GGAGTCTGTG GATGACGGGA AGCTGTCGGT GGTCATGTCA
    CCTGATGAAG ACAGGAAGAG TTCTCCGGCG TATTCACTCA AGTCTATGAA TCTGGGAATG
    TCTGTGGACT CATCGCAGGT TCACGTACAG ATGCGTTATC CAGAGCAGCG GCCGTACCTG
    CGCAGACAGA GCACCAGCGG ATCACACTTC TCCCACAGAT CCTCCGCACG CTCCAAACGC
    GGCAGCACAA GCACACGTCA CAGCACCCAT GGGGAAAACA GCACAACAGA GTCAGAAGTA
    CCACAAAAGA GCCGGAAAGA AATGATTATG GAGAAAATCA GAGAGCAGCT CATCAAAGCA
    AAGGTTTACC TGCTCAAAAG ATTGGTTGAG TTTTATCAGC CCATCCGTCA GTTCTTCTAC
    AACCTGGTTC ATCCGGAGTA CAGCGCCGTG ACGGATGTTT ACGTATTGAT GTTTCTAGCA
    GACACTGTAG ATTTTATCAT CATCGTCTTT GGCTTTTGGG CATTTGGGAA ACATCAGGGT
    GGTGCTGACA TCACTTCCTC CCTGTCAGAG GACCAGGTTC CTGGGCCGTT CCTTGTTATG
    GTTCTCATAC AGTTTGGGAC GATGGTAGTG GATCGAGCCC TGTATCTGCG CAAGACTGTT
    GTGGGAAAAG TGATTTTTCA GGTCATCCTG GTGTTTGGCA TCCATTTCTG GATGTTTTTC
    ATCCTGCCGG GAATCACAGA GAGGCGCTTC AGTCAGAACA CTATCGCTCA GCTCTGGTAC
    TTTGTGAAGT GCATTTACTT CGGCCTGTCA GCCTATCAGA TCAGATGTGG ATATCCAACC
    CGCATCCTGG GAAACTTCCT CACCAAGAGC TACAACTACG CCAACCTCTT CCTCTTCCAG
    GGTTTCCGTC TGATCCCGTT CCTGACGGAG CTGCGTGCGG TGATGGACTG GGTCTGGACG
    GACACAACGC TCAGCTTGTC CAGCTGGATC TGTGTGGAGG ACATCTATGC TCACATCTTC
    ATCCTCAAAT GCTGGAGGGA GTCTGAGAAG CGTTACCCTC AGCCGCGCGG GCAGAAGAAG
    AAGAAGGTGG TGAAGTATGG GATGGGCGGC ATGATCGTGA TGCTCCTGAT CTGCATCGTC
    TGGTTTCCTC TGCTCTTCAT GTCTCTGGTC AAATCTGTGG CAGGAGTGGT TAACAAGCCT
    CTGGAGGTGT CCGTCTCGCT CACGCTCGGA GGACTCCAGC CGATCTTCAC AATGAGTGCC
    CAGCAGAACC ATATCAAATC CGTCACTGGG GCAGAATTTA AGAAGTTCAC AGATCACTAC
    AAGTCTAACG CAAATGCCAT CATGTTCCTG GAGTCGTATT TTCCTGAGGA TCTGACAATA
    GCACATCTAG AGGGAAGTTC AAACTCACTC TGGACCATCA GCCCCCCGAG CAAGGAAAAC
    CTGAAGAATA TGCTCAATGA CACGAGAGAG CCGTTTCCTC TCACCTTCAC CTGGTCCATT
    CAAAGGAACC TCAGTTTAGG TGCCAAAGCT GAAACTGCAA TGGGGAAATA CTTCATTGAG
    CTGGACGACG AGACTAGAAA AGGTCTAACT GATCAACTGG GGAATAGCAG CCAGCGTGTG
    ACAATCAAAA AAATATTTCC AAGGTACATC AGAGCACCAA GCGACTCCAA TGCCAAACCT
    ATTGACCAGC TTTACAACGA TGGAGAGTAC ATGGACATCA CACTGTTTTT AAATAAGAAG
    AACAGCTCAG AAGGCGTTCA GGAGTGGTGG ATCGTCAATC AGACAGATTC AGGTCCATTA
    CCTGGACTCA ACGGGTCTGG TCTTCAGATC TACATCATCA GTGACCAAGT CAGTCCACCC
    AGTCTGGGCT TTCTGGCTGG TTATGGGATC ATGGGTCTGT ACATGTCAGT GGTTTTGGTG
    ATCGGGAAGT TTGTGCGCGA GTTCTTCAGC GGGATCTCGC ACACCATCAT GTTCGAAGAG
    CTCCCTAACG TGGACCGAAT CCTCAAACTC TGCACCGATA TCTTCCTGGT CCGAGAGACG
    GGCGAACTGG ACCTGGAGGA AGACCTTTAC GCCAAACTCA TCTTCCTCTA CCGCTCTCCA
    GAAACCATGA TCAAATGGAC CAGAGAGAAG AGTGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56506
    Clone ID Related Accession (Same CDS sequence) XM_017352447.1
    Accession Version XM_017352447.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product piezo-type mechanosensitive ion channel component 2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGTTCATGG CCAGTCTGGG TGTGTGGCTC CTGTGCCGAA AACTGCTCCA CCAACGGCCC 
    ACTGAGGATA TGGCCCAGGA CAACCAGCAA TTCCTGTCTG AGGAGAAGGA GGAGGAAAAC
    GAGAAGCTGG AAAATGAAGA AGATGAGAAT GATAATAATG ATGATGATGA TGATGAAATG
    CTGTTTGACG AGGACTTTGA TGCAGAAGAT GAAGCAGAGG AAGAAGATGG AGAAGCCGGA
    AGACTGGAGG AGGAGGAGGA GGAAGAGGAG GAGGAAGAGG AAGTGCAGGA GAGCACCAAG
    ATGAAGATCC TGCGCGCGGT TGGAGGAGTC GCTGCCAAAC TCAAGGAGAT CATCGGGAAC
    CTCATCACTA CTGCTGGACA GGTGGTGGTC ACTATCATGC TGGGAATGAC AGGTGTGACG
    CTGCCTTCTC TGTCCTCCGC TGTGTATTTC TTCGTGTTTC TGGGTCTGTG CACATGGTGG
    TCTCTCTGTA AGACCTTCGA TAAGCTCTTG TTCAGCTGTC TGTGTGTCCT GATGGCCATT
    TTCAGCGCTG GACATCTCAT CGTGCTTTAT ATCAACCAGT TTCAGTTCCT CCAGGAAGCC
    ATAAGTCCCA CCGATGGATA TACCAGTGTT TTTGGCATCT CCTCCATCAT TTACACAGAC
    TGCTCCTCTA CATGGAAGCT TGCTGTCAGG AGTGGACTCA AATGGCATCA TTTCGTCAAT
    CCCATAATGA TCCTGGTCCT GTATTACACA CTGGCCACGC TGATCCGCCT GTGGCTGCAG
    GATCCTATGA TGATGGAGGA CAGCGAGGAC GGAGAGGTGA TCGAGGGCAA TGAAGCCATG
    GAAAACAACG TCCATCACAC GTCCATCCGG AAACGGCTGC TCTGGTGGAA AGCGCATCAG
    AAAACCGAGG AGAGAAATCT GATCTCCACT CAGGATGGGT ACAGCACCTC GGAGGTGTTA
    GTGGTCACCT CCAATGGGAC GTCGTTTGAT TATGTGTCCG CCGTGCCCAT CGAGAACGGA
    CCTGTCAGTC TGGACATTTA CTCCACTCCT CAGTACAAAG TGGATCAGTC TGTCGAATAT
    GACGAGAAGA CGGATGAGAA CACAAATGAG GTGCTGGACT CACAGCTGGA GGAGGAAGAG
    GAGAAGACAG ATGAGGAAGA GGAAGCCGAA GAGGAAGAGC AGGGCGGACC ACCGGCAGCT
    CTCGTCAAAG TGTTCAAGTT CATCATGAAG CAGAGCTACA TCTGCGCTCT CATCGCTATG
    ATGGCGTGGA GTATAACGTA TGTCAGCTGG CTGACGTTTG TCTTTCTGAT CTGGTCCTGT
    ACTCTGTGGA TGGTGCGCGA CCGGAGGAAA TACGCCATGC TGACTTCGCC ATTCATGGTG
    GCTTATGGGA ACTTCCTGTT GGTCCTTCAG TATATCTGGT GTTTTGAGAA GTTGAAGCCG
    GTGTCTGGTT TCTTTGTGAA AATGGAGGTG CCTTTCACTG AACTGAGCAC CAAGGTTCTG
    TGTCAGCTGA GTTTCTGGCT ACTGCTCAGA CAAACTTTAA TGGAGAGACA AGAAAGGAAG
    GAAGAAGAAG CAGCACTTTC TGACATCAAA GTAGACGAGC AGAAAAAAGT GGAAGAAAAA
    AGTGAAGAGG AAGTGGATGA GAACGACCTG ATGCAAGTGC TGGGCAAGCT GGTGATGGCT
    CTGCTGGTCA AATACTGGAT CTACGTCTGT GGAGGAATGT TCTTCTTCGT CAGTTTTGAG
    GGAGATATGG CGACATATAA GATCATCTAC ATGATGATGC TGCTGTCCTG CGTCGCTCTT
    TACCAGGTAC ATTATGAATA CTGGCGCAGG ATCCTGAAGT ATTTCTGGAT GTCTGTGGTG
    GTTTACACTA TGCTGGTGCT CATCCTGATC TACACCAGCC AGTTTGAGGA TGGCATCCCG
    ACCTGGACCA GGATGACCGG CCTGGACAAA GATGTACTGA AGGATATCGG TCTGGAGAAG
    TTCAGCCTCT CTAATTTGTT CACGCGTATC TTCATCCCGA CGTCTTTCCT GTTGGTGTGT
    ATCCTGCATC TGCACTACTT CCATGACCAT TTTCTGGAGC TGACTGATCT GAAAGCCGTG
    GTCAGCAAAC AGGAGAGCAC CATTTACAGA TTGGCTCATC CCGATGGGAG TTTGGCGGAT
    CTGACCATTG TGAGCTCGTC TCCGGAGCCG CTGCTGAAGG ATGAGAAGGA AAAAGAGTCT
    CTGAATGAGG TGCTGGTGGT GGATTGTGTG GGGGAAGAGA AGAAGAAAGA CTCTTTATCC
    ATTTATTCGG CTCAAATGTC CAGTCAGGAG GACGTTCATC AGTCCAGTGC TGCCACAGAC
    CCTGAACTGC CTTCAGAACA AAGCTCAGAG ATGAAGAATA AGTGGCATCT GGTGGTGGAC
    CGGCTCACAG TGCTGTTCCT CAAGTTCCTG GAGTACTTTC ATAAGCTGAA ACTCTTTGTC
    TGGTGGCTGC TGGAGATGCA CATCATCAAG ATCGTGGCCA CGTACATCAT CCTGCTCTCG
    GTCAAAGAGG TCTCGCTGCT GAACTTTGTG TTTTTGATCT CCTGGGCGTT TGCGCTGCCG
    TATAAGCAGT TTCGAGTTCT CTCCTCCAGC GTCTGCACCG TATGGACGTG CGTTATTATT
    GTCTGCAAGC TCTTTTACCA GCTCAAAACC ATCAATCCTG CCAACTACTC CAGTGATTGT
    ATTATGCCCA ACTATACAGA AACAGAAAAA AAGGACATGA AGACGTCTCT ACTGTATCGT
    GATCCAGTGG ATCCTGCTAA CTGGGTTGGC CTGCGGAAGT TTGATCATGG GATAATATTG
    CCTAACCTGA GGAATAACTT GCTGATGTTG GCCCTCTTGG CGTTCGAGGT TACCATTTAC
    AGACACCAGG AGTTTTTCCG TCTAAGAAAC AAACTTTCTC CTCCTCCTTC ACGCACCATC
    TTTCATGACA TCACCCGGCA ACACCTTGAC AACGGCATCA TCGAGTGCGC CAAGTACTTT
    ATTAACTACT TCTTCTACAA GTTTGGTTTA GAGGTGTGTT TTTTGTTGGC CATCAATGTG
    ATGGGTCAGC GGATGGATTT CTACTCCATG GTCCACGGCT TGGCTCTGGC TGTGGTGATG
    TTCAGACGGC GGAGGAAAGC CATCGCTGAG ATCTGGCCCA AATACTGCTG CTTCCTGGCC
    TGTATGATCA CCTTTCAGTA TTTCATATGC ATCGGGATTC CACCGGCTGC TTGTGCAGAT
    TATCCCTGGA GGTTTCCCAA CTCCTCGATG ACGTCTAATG TCATTAAATG GCTTTACCTT
    CCTGATTTCC ACACTCCACC CAGCTCCATG TTCCTCGGCT ATGATTTCAT GCTGCTGCTG
    TGTGCGTCGC TGCAGAGGCA GGTGTTTGAT GAAGAAAATA AAGCCGCCGT TCGCCTGATG
    GCGGGAGATA ATGTGGAGAT CTGCCGAGAT CTGGACGCTG CATCATTCAG TGTTCACAAC
    CCGGTGCCAG ACTTCATTCA CTGCAGGTCA TACCTGGACA TGCTGAAGGT GATCATGTTC
    AGTTACCTGT TCTGGTTCGT CCTGACCATC ATCTTCATCA CGGGCACCAC ACGGATCAGC
    ATCTTCTGCA TGGGTTACCT GGTGGCCTGC TTTTACTTCC TGCTGTTTGG TGGGCAACTT
    TTACTCAAGC CAATCAAAGA GATCCTCCGT TACTGGGACT TTCTCATCGC CTACAACGTT
    TTCGTCATCA CCATGAAGAA CGTCTTTGCA ATTTTGGCGT GCGGTTACAT CAACTCGCTG
    GTGAAGAATA GCTGCTGGTT AATTCAGCTG CTCAGTCTGG CCTGCACCAT TAAAGACTAC
    CAACCAAAAA CAGATCTGCC TGGAGTTGAC TGTGAGCTGC CAAAAGACGA GGCCGGGATC
    ATCTGGGACA GCATCTGCTT CACCTTCCTC CTCCTGCAGA GAAGAGTCTT CATGAGCTAC
    TATTTCTTGC ATGTAATCGC AGACATTCGA GCCGGACAGA TTCTTGCATC CCGAGGAGCT
    GAGTTGTTTC AGGCCAGTAT CGTGAAGGCT GTGAGAGCTC GTCTGGAGGA GGAGAAGAAA
    TCCATGGAGC AACTGAAGAG ACAGATGGAT CGTATTAAAA CGAGACAGCA GAAGTTCAAG
    AGAGGAAAAG AGAAGATGCT CAGCATAGCC CAAGAGTCTG GAGACGGAGA GAAACTCATC
    CCACCAGTGG ACAATGAGGA GGAGGAGGAC GAGGAGAAAA AGAAAAACAA GGTGCAAAAA
    AAGCAGTGGT GGAGGCCTTG GGTTGACCAT GCGTCCATGG TTAGAAGTGG TGATTATTAC
    CTGTTTGAAA CTGACAGTGA AGAGGAGGAG GAAGAGGAGG AGAAAAAAGA CGAAGAGCCT
    CCCAAAAGAT CAGCCTTTCA GCGAGCGATC AGAAAGTTTG CTTCAGCCCT TGTTGCTTTA
    CCCAGATCAG TCATTAAACT GCCCAAAACT ATTCTGCAGT ATTTAATAAG GGCAGCCAAG
    TTTGTGTATC ATACCTGGAT CGCCGAATCT AAAGCAGCTC TGAAAGAAAG AGGAAAAGGC
    AAGCGCTTCT GGAAGCGATA CGGTCGCCGG CTTAAAAAAG AGAAGAAAGA AGAACATGTG
    GCGATAGAGA TCGGTGAAGA CGAGCGTCAG AGTTCAGAAG AGGAGAAGAC AGATGGACCA
    GACAACATCA TCAAGAGGGT GTTCAACATC ATCAAGTTCA CCTGGGCTCT GTTTCTGACC
    ACCGTGGAGA GCATCACCAA ATGGCTGAAC TCAGTCTGCA GAGAATATAT CGACATCTCC
    ACCGTCCTGC GCATCGAGCG CTGCATGCTG ACCCGAGAAG TCAAAAAGGG GAATGTGCCG
    TCCCGTGAGA GCATCCATGT GTACTACCAG AAGCAGATGA AGCTGAATGG ATCTCGTGAA
    TCTGGTCTGG ACCGCATCAG TGAGGAGGAT TCAGCCTCAA ACAGAGAACG CCGCAGACGC
    GCACAACACA GTCTGGATTC ATTCGCTTCC AGAGACAGCA TCTCCAGCGC ATACACCGAA
    GCCACCATGC TGTTTTCCCG CCAGTCCACC TTGGACGATT TGGACGACAT GCCTCAGAAT
    ATTCCCAAAA CCAGCGAACG CGCTCGACCC AAACTGCGCA AAATGTACGG CCTCGACATG
    TCCAACTCCT CTGCGGACAG CGGAAGCAGC GTCATCTCCA GTGAGGCTAC TCAGTGCGTC
    ACGCTGTTTT CTCGGCAAGG AACCAACGAC ACTATCGACG AGGCTGAAGA CGAGCGAGAA
    CAGCTGGAGC ACAAGCAGAA AACAGAAGAA GCAGAACTCA GAGACAAACC TGAAGAAGAG
    CAGGAAGTGG AAATAAGCGA TCAGGGGAAA CAGGAAGTGT TACCGGTGAG CCATCTGGTG
    ACACAGGAAG TGAAAGAGGA TGTACTACCT GTGAGCGATC AGCTGACTGG GGAAGTGACT
    GAGGAAGTTG AAGAGGAGGT GGAAGTAGTT AAGGAAATGG AAGAGGAAGT AGAAAAGGAA
    ATTGGAGCAA AGGTGGAAAA GGAAGTAGAA GCGGAAAAGG AAGAGGAAGC GGAAAAGGAA
    GTGGGTGAGG AAGTGCAATG GGACTTGGTG GATCCAGAGG AAGCAGAGGA GTGTGTAAGT
    GATGGGGACG GTGGTGAGCA GATGCAGCAG GATGAAGGTG AATGGTCGGG AGTGGAGAAC
    GTGGTGAGCG GTCCGCTGTT CACACCAGAC ACTGACGCCC CCAACACGTC TGACGCTGAC
    GTGCCGCCCA GCTACAGTAA AGCCGTGAGC TTCGACCGGC TGTCTGTGAG CTCGGACGAC
    AGTGACAGCG ACAAACGGCT GATGCTAATG ACGCCTGACA GCAAATCTGA CCTGGACGAC
    CCTCTGCTGC CCTCCATGAC CACCGACATG ACCGCCAGCG AACTGCTGCT CAACAAAATG
    TTCTACGACG AGATGCTGGA CAGCTCAGAT CGCTTCTATA AGTCTCAGCC GCTGGGTCTC
    CAGTTGTGTT ACGCTCTCTA CAACCTGCTG GTGGCTCACT CAGAAATGGT GTGTTATCTG
    GTCATCATCC TCAACCACAT GATCTCCGCC TCCATGGCCA CGCTGGTGCT GCCCATCCTC
    ATCTTCCTGT GGGCCATGCT GTCCGTTCCG CGACCCAGCA AGAGGTTCTG GATGACCGCC
    ATCGTCTACA CTGAGGTCAC CATCGTCATC AAATACTTCT TCCAGTTCAG CTTTTTCCCT
    TTCAATCAGA ACCTGGAGGT GAACAAGGGC AAGCCGTACC ACCCGCCCAA CATCCTGGGC
    ACAGAGAAAA AAGACGGATA CGTGCACTAC GACCTGGTCC AGCTGCTGGC GCTCTTCTTC
    CACAGATCCA TACTAAAGTG TCATGGTCTC TGGGACGAGG ACGACCCCAA AGTGCCCCGT
    AAGGATGAGT GTCCTCGTCA GGAGTCTGTG GATGACGGGA AGCTGTCGGT GGTCATGTCA
    CCTGATGAAG ACAGGAAGAG TTCTCCGGCG TATTCACTCA AGTCTATGAA TCTGGGAATG
    TCTGTGGACT CATCGCAGGT TCACGTACAG ATGCGTTATC CAGAGCAGCG GCCGTACCTG
    CGCAGACAGA GCACCAGCGG ATCACACTTC TCCCACAGAT CCTCCGCACG CTCCAAACGC
    GGCAGCACAA GCACACGTCA CAGCACCCAT GGGGAAAACA GCACAACAGA GTCAGAAGTA
    CCACAAAAGA GCCGGAAAGA AATGATTATG GAGAAAATCA GAGAGCAGCT CATCAAAGCA
    AAGGTTTACC TGCTCAAAAG ATTGGTTGAG TTTTATCAGC CCATCCGTCA GTTCTTCTAC
    AACCTGGTTC ATCCGGAGTA CAGCGCCGTG ACGGATGTTT ACGTATTGAT GTTTCTAGCA
    GACACTGTAG ATTTTATCAT CATCGTCTTT GGCTTTTGGG CATTTGGGAA ACATCAGGGT
    GGTGCTGACA TCACTTCCTC CCTGTCAGAG GACCAGGTTC CTGGGCCGTT CCTTGTTATG
    GTTCTCATAC AGTTTGGGAC GATGGTAGTG GATCGAGCCC TGTATCTGCG CAAGACTGTT
    GTGGGAAAAG TGATTTTTCA GGTCATCCTG GTGTTTGGCA TCCATTTCTG GATGTTTTTC
    ATCCTGCCGG GAATCACAGA GAGGCGCTTC AGTCAGAACA CTATCGCTCA GCTCTGGTAC
    TTTGTGAAGT GCATTTACTT CGGCCTGTCA GCCTATCAGA TCAGATGTGG ATATCCAACC
    CGCATCCTGG GAAACTTCCT CACCAAGAGC TACAACTACG CCAACCTCTT CCTCTTCCAG
    GGTTTCCGTC TGATCCCGTT CCTGACGGAG CTGCGTGCGG TGATGGACTG GGTCTGGACG
    GACACAACGC TCAGCTTGTC CAGCTGGATC TGTGTGGAGG ACATCTATGC TCACATCTTC
    ATCCTCAAAT GCTGGAGGGA GTCTGAGAAG CGTTACCCTC AGCCGCGCGG GCAGAAGAAG
    AAGAAGGTGG TGAAGTATGG GATGGGCGGC ATGATCGTGA TGCTCCTGAT CTGCATCGTC
    TGGTTTCCTC TGCTCTTCAT GTCTCTGGTC AAATCTGTGG CAGGAGTGGT TAACAAGCCT
    CTGGAGGTGT CCGTCTCGCT CACGCTCGGA GGACTCCAGC CGATCTTCAC AATGAGTGCC
    CAGCAGAACC ATATCAAATC CGTCACTGGG GCAGAATTTA AGAAGTTCAC AGATCACTAC
    AAGTCTAACG CAAATGCCAT CATGTTCCTG GAGTCGTATT TTCCTGAGGA TCTGACAATA
    GCACATCTAG AGGGAAGTTC AAACTCACTC TGGACCATCA GCCCCCCGAG CAAGGAAAAC
    CTGAAGAATA TGCTCAATGA CACGAGAGAG CCGTTTCCTC TCACCTTCAC CTGGTCCATT
    CAAAGGAACC TCAGTTTAGG TGCCAAAGCT GAAACTGCAA TGGGGAAATA CTTCATTGAG
    CTGGACGACG AGACTAGAAA AGGTCTAACT GATCAACTGG GGAATAGCAG CCAGCGTGTG
    ACAATCAAAA AAATATTTCC AAGGTACATC AGAGCACCAA GCGACTCCAA TGCCAAACCT
    ATTGACCAGC TTTACAACGA TGGAGAGTAC ATGGACATCA CACTGTTTTT AAATAAGAAG
    AACAGCTCAG AAGGCGTTCA GGAGTGGTGG ATCGTCAATC AGACAGATTC AGGTCCATTA
    CCTGGACTCA ACGGGTCTGG TCTTCAGATC TACATCATCA GTGACCAAGT CAGTCCACCC
    AGTCTGGGCT TTCTGGCTGG TTATGGGATC ATGGGTCTGT ACATGTCAGT GGTTTTGGTG
    ATCGGGAAGT TTGTGCGCGA GTTCTTCAGC GGGATCTCGC ACACCATCAT GTTCGAAGAG
    CTCCCTAACG TGGACCGAAT CCTCAAACTC TGCACCGATA TCTTCCTGGT CCGAGAGACG
    GGCGAACTGG ACCTGGAGGA AGACCTTTAC GCCAAACTCA TCTTCCTCTA CCGCTCTCCA
    GAAACCATGA TCAAATGGAC CAGAGAGAAG AGTGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207936.1
    CDS59..8617
    Translation

    Target ORF information:

    RefSeq Version XM_017352447.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio piezo type mechanosensitive ion channel component 2 (piezo2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGTTCATGG CCAGTCTGGG TGTGTGGCTC CTGTGCCGAA AACTGCTCCA CCAACGGCCC 
    ACTGAGGATA TGGCCCAGGA CAACCAGCAA TTCCTGTCTG AGGAGAAGGA GGAGGAAAAC
    GAGAAGCTGG AAAATGAAGA AGATGAGAAT GATAATAATG ATGATGATGA TGATGAAATG
    CTGTTTGACG AGGACTTTGA TGCAGAAGAT GAAGCAGAGG AAGAAGATGG AGAAGCCGGA
    AGACTGGAGG AGGAGGAGGA GGAAGAGGAG GAGGAAGAGG AAGTGCAGGA GAGCACCAAG
    ATGAAGATCC TGCGCGCGGT TGGAGGAGTC GCTGCCAAAC TCAAGGAGAT CATCGGGAAC
    CTCATCACTA CTGCTGGACA GGTGGTGGTC ACTATCATGC TGGGAATGAC AGGTGTGACG
    CTGCCTTCTC TGTCCTCCGC TGTGTATTTC TTCGTGTTTC TGGGTCTGTG CACATGGTGG
    TCTCTCTGTA AGACCTTCGA TAAGCTCTTG TTCAGCTGTC TGTGTGTCCT GATGGCCATT
    TTCAGCGCTG GACATCTCAT CGTGCTTTAT ATCAACCAGT TTCAGTTCCT CCAGGAAGCC
    ATAAGTCCCA CCGATGGATA TACCAGTGTT TTTGGCATCT CCTCCATCAT TTACACAGAC
    TGCTCCTCTA CATGGAAGCT TGCTGTCAGG AGTGGACTCA AATGGCATCA TTTCGTCAAT
    CCCATAATGA TCCTGGTCCT GTATTACACA CTGGCCACGC TGATCCGCCT GTGGCTGCAG
    GATCCTATGA TGATGGAGGA CAGCGAGGAC GGAGAGGTGA TCGAGGGCAA TGAAGCCATG
    GAAAACAACG TCCATCACAC GTCCATCCGG AAACGGCTGC TCTGGTGGAA AGCGCATCAG
    AAAACCGAGG AGAGAAATCT GATCTCCACT CAGGATGGGT ACAGCACCTC GGAGGTGTTA
    GTGGTCACCT CCAATGGGAC GTCGTTTGAT TATGTGTCCG CCGTGCCCAT CGAGAACGGA
    CCTGTCAGTC TGGACATTTA CTCCACTCCT CAGTACAAAG TGGATCAGTC TGTCGAATAT
    GACGAGAAGA CGGATGAGAA CACAAATGAG GTGCTGGACT CACAGCTGGA GGAGGAAGAG
    GAGAAGACAG ATGAGGAAGA GGAAGCCGAA GAGGAAGAGC AGGGCGGACC ACCGGCAGCT
    CTCGTCAAAG TGTTCAAGTT CATCATGAAG CAGAGCTACA TCTGCGCTCT CATCGCTATG
    ATGGCGTGGA GTATAACGTA TGTCAGCTGG CTGACGTTTG TCTTTCTGAT CTGGTCCTGT
    ACTCTGTGGA TGGTGCGCGA CCGGAGGAAA TACGCCATGC TGACTTCGCC ATTCATGGTG
    GCTTATGGGA ACTTCCTGTT GGTCCTTCAG TATATCTGGT GTTTTGAGAA GTTGAAGCCG
    GTGTCTGGTT TCTTTGTGAA AATGGAGGTG CCTTTCACTG AACTGAGCAC CAAGGTTCTG
    TGTCAGCTGA GTTTCTGGCT ACTGCTCAGA CAAACTTTAA TGGAGAGACA AGAAAGGAAG
    GAAGAAGAAG CAGCACTTTC TGACATCAAA GTAGACGAGC AGAAAAAAGT GGAAGAAAAA
    AGTGAAGAGG AAGTGGATGA GAACGACCTG ATGCAAGTGC TGGGCAAGCT GGTGATGGCT
    CTGCTGGTCA AATACTGGAT CTACGTCTGT GGAGGAATGT TCTTCTTCGT CAGTTTTGAG
    GGAGATATGG CGACATATAA GATCATCTAC ATGATGATGC TGCTGTCCTG CGTCGCTCTT
    TACCAGGTAC ATTATGAATA CTGGCGCAGG ATCCTGAAGT ATTTCTGGAT GTCTGTGGTG
    GTTTACACTA TGCTGGTGCT CATCCTGATC TACACCAGCC AGTTTGAGGA TGGCATCCCG
    ACCTGGACCA GGATGACCGG CCTGGACAAA GATGTACTGA AGGATATCGG TCTGGAGAAG
    TTCAGCCTCT CTAATTTGTT CACGCGTATC TTCATCCCGA CGTCTTTCCT GTTGGTGTGT
    ATCCTGCATC TGCACTACTT CCATGACCAT TTTCTGGAGC TGACTGATCT GAAAGCCGTG
    GTCAGCAAAC AGGAGAGCAC CATTTACAGA TTGGCTCATC CCGATGGGAG TTTGGCGGAT
    CTGACCATTG TGAGCTCGTC TCCGGAGCCG CTGCTGAAGG ATGAGAAGGA AAAAGAGTCT
    CTGAATGAGG TGCTGGTGGT GGATTGTGTG GGGGAAGAGA AGAAGAAAGA CTCTTTATCC
    ATTTATTCGG CTCAAATGTC CAGTCAGGAG GACGTTCATC AGTCCAGTGC TGCCACAGAC
    CCTGAACTGC CTTCAGAACA AAGCTCAGAG ATGAAGAATA AGTGGCATCT GGTGGTGGAC
    CGGCTCACAG TGCTGTTCCT CAAGTTCCTG GAGTACTTTC ATAAGCTGAA ACTCTTTGTC
    TGGTGGCTGC TGGAGATGCA CATCATCAAG ATCGTGGCCA CGTACATCAT CCTGCTCTCG
    GTCAAAGAGG TCTCGCTGCT GAACTTTGTG TTTTTGATCT CCTGGGCGTT TGCGCTGCCG
    TATAAGCAGT TTCGAGTTCT CTCCTCCAGC GTCTGCACCG TATGGACGTG CGTTATTATT
    GTCTGCAAGC TCTTTTACCA GCTCAAAACC ATCAATCCTG CCAACTACTC CAGTGATTGT
    ATTATGCCCA ACTATACAGA AACAGAAAAA AAGGACATGA AGACGTCTCT ACTGTATCGT
    GATCCAGTGG ATCCTGCTAA CTGGGTTGGC CTGCGGAAGT TTGATCATGG GATAATATTG
    CCTAACCTGA GGAATAACTT GCTGATGTTG GCCCTCTTGG CGTTCGAGGT TACCATTTAC
    AGACACCAGG AGTTTTTCCG TCTAAGAAAC AAACTTTCTC CTCCTCCTTC ACGCACCATC
    TTTCATGACA TCACCCGGCA ACACCTTGAC AACGGCATCA TCGAGTGCGC CAAGTACTTT
    ATTAACTACT TCTTCTACAA GTTTGGTTTA GAGGTGTGTT TTTTGTTGGC CATCAATGTG
    ATGGGTCAGC GGATGGATTT CTACTCCATG GTCCACGGCT TGGCTCTGGC TGTGGTGATG
    TTCAGACGGC GGAGGAAAGC CATCGCTGAG ATCTGGCCCA AATACTGCTG CTTCCTGGCC
    TGTATGATCA CCTTTCAGTA TTTCATATGC ATCGGGATTC CACCGGCTGC TTGTGCAGAT
    TATCCCTGGA GGTTTCCCAA CTCCTCGATG ACGTCTAATG TCATTAAATG GCTTTACCTT
    CCTGATTTCC ACACTCCACC CAGCTCCATG TTCCTCGGCT ATGATTTCAT GCTGCTGCTG
    TGTGCGTCGC TGCAGAGGCA GGTGTTTGAT GAAGAAAATA AAGCCGCCGT TCGCCTGATG
    GCGGGAGATA ATGTGGAGAT CTGCCGAGAT CTGGACGCTG CATCATTCAG TGTTCACAAC
    CCGGTGCCAG ACTTCATTCA CTGCAGGTCA TACCTGGACA TGCTGAAGGT GATCATGTTC
    AGTTACCTGT TCTGGTTCGT CCTGACCATC ATCTTCATCA CGGGCACCAC ACGGATCAGC
    ATCTTCTGCA TGGGTTACCT GGTGGCCTGC TTTTACTTCC TGCTGTTTGG TGGGCAACTT
    TTACTCAAGC CAATCAAAGA GATCCTCCGT TACTGGGACT TTCTCATCGC CTACAACGTT
    TTCGTCATCA CCATGAAGAA CGTCTTTGCA ATTTTGGCGT GCGGTTACAT CAACTCGCTG
    GTGAAGAATA GCTGCTGGTT AATTCAGCTG CTCAGTCTGG CCTGCACCAT TAAAGACTAC
    CAACCAAAAA CAGATCTGCC TGGAGTTGAC TGTGAGCTGC CAAAAGACGA GGCCGGGATC
    ATCTGGGACA GCATCTGCTT CACCTTCCTC CTCCTGCAGA GAAGAGTCTT CATGAGCTAC
    TATTTCTTGC ATGTAATCGC AGACATTCGA GCCGGACAGA TTCTTGCATC CCGAGGAGCT
    GAGTTGTTTC AGGCCAGTAT CGTGAAGGCT GTGAGAGCTC GTCTGGAGGA GGAGAAGAAA
    TCCATGGAGC AACTGAAGAG ACAGATGGAT CGTATTAAAA CGAGACAGCA GAAGTTCAAG
    AGAGGAAAAG AGAAGATGCT CAGCATAGCC CAAGAGTCTG GAGACGGAGA GAAACTCATC
    CCACCAGTGG ACAATGAGGA GGAGGAGGAC GAGGAGAAAA AGAAAAACAA GGTGCAAAAA
    AAGCAGTGGT GGAGGCCTTG GGTTGACCAT GCGTCCATGG TTAGAAGTGG TGATTATTAC
    CTGTTTGAAA CTGACAGTGA AGAGGAGGAG GAAGAGGAGG AGAAAAAAGA CGAAGAGCCT
    CCCAAAAGAT CAGCCTTTCA GCGAGCGATC AGAAAGTTTG CTTCAGCCCT TGTTGCTTTA
    CCCAGATCAG TCATTAAACT GCCCAAAACT ATTCTGCAGT ATTTAATAAG GGCAGCCAAG
    TTTGTGTATC ATACCTGGAT CGCCGAATCT AAAGCAGCTC TGAAAGAAAG AGGAAAAGGC
    AAGCGCTTCT GGAAGCGATA CGGTCGCCGG CTTAAAAAAG AGAAGAAAGA AGAACATGTG
    GCGATAGAGA TCGGTGAAGA CGAGCGTCAG AGTTCAGAAG AGGAGAAGAC AGATGGACCA
    GACAACATCA TCAAGAGGGT GTTCAACATC ATCAAGTTCA CCTGGGCTCT GTTTCTGACC
    ACCGTGGAGA GCATCACCAA ATGGCTGAAC TCAGTCTGCA GAGAATATAT CGACATCTCC
    ACCGTCCTGC GCATCGAGCG CTGCATGCTG ACCCGAGAAG TCAAAAAGGG GAATGTGCCG
    TCCCGTGAGA GCATCCATGT GTACTACCAG AAGCAGATGA AGCTGAATGG ATCTCGTGAA
    TCTGGTCTGG ACCGCATCAG TGAGGAGGAT TCAGCCTCAA ACAGAGAACG CCGCAGACGC
    GCACAACACA GTCTGGATTC ATTCGCTTCC AGAGACAGCA TCTCCAGCGC ATACACCGAA
    GCCACCATGC TGTTTTCCCG CCAGTCCACC TTGGACGATT TGGACGACAT GCCTCAGAAT
    ATTCCCAAAA CCAGCGAACG CGCTCGACCC AAACTGCGCA AAATGTACGG CCTCGACATG
    TCCAACTCCT CTGCGGACAG CGGAAGCAGC GTCATCTCCA GTGAGGCTAC TCAGTGCGTC
    ACGCTGTTTT CTCGGCAAGG AACCAACGAC ACTATCGACG AGGCTGAAGA CGAGCGAGAA
    CAGCTGGAGC ACAAGCAGAA AACAGAAGAA GCAGAACTCA GAGACAAACC TGAAGAAGAG
    CAGGAAGTGG AAATAAGCGA TCAGGGGAAA CAGGAAGTGT TACCGGTGAG CCATCTGGTG
    ACACAGGAAG TGAAAGAGGA TGTACTACCT GTGAGCGATC AGCTGACTGG GGAAGTGACT
    GAGGAAGTTG AAGAGGAGGT GGAAGTAGTT AAGGAAATGG AAGAGGAAGT AGAAAAGGAA
    ATTGGAGCAA AGGTGGAAAA GGAAGTAGAA GCGGAAAAGG AAGAGGAAGC GGAAAAGGAA
    GTGGGTGAGG AAGTGCAATG GGACTTGGTG GATCCAGAGG AAGCAGAGGA GTGTGTAAGT
    GATGGGGACG GTGGTGAGCA GATGCAGCAG GATGAAGGTG AATGGTCGGG AGTGGAGAAC
    GTGGTGAGCG GTCCGCTGTT CACACCAGAC ACTGACGCCC CCAACACGTC TGACGCTGAC
    GTGCCGCCCA GCTACAGTAA AGCCGTGAGC TTCGACCGGC TGTCTGTGAG CTCGGACGAC
    AGTGACAGCG ACAAACGGCT GATGCTAATG ACGCCTGACA GCAAATCTGA CCTGGACGAC
    CCTCTGCTGC CCTCCATGAC CACCGACATG ACCGCCAGCG AACTGCTGCT CAACAAAATG
    TTCTACGACG AGATGCTGGA CAGCTCAGAT CGCTTCTATA AGTCTCAGCC GCTGGGTCTC
    CAGTTGTGTT ACGCTCTCTA CAACCTGCTG GTGGCTCACT CAGAAATGGT GTGTTATCTG
    GTCATCATCC TCAACCACAT GATCTCCGCC TCCATGGCCA CGCTGGTGCT GCCCATCCTC
    ATCTTCCTGT GGGCCATGCT GTCCGTTCCG CGACCCAGCA AGAGGTTCTG GATGACCGCC
    ATCGTCTACA CTGAGGTCAC CATCGTCATC AAATACTTCT TCCAGTTCAG CTTTTTCCCT
    TTCAATCAGA ACCTGGAGGT GAACAAGGGC AAGCCGTACC ACCCGCCCAA CATCCTGGGC
    ACAGAGAAAA AAGACGGATA CGTGCACTAC GACCTGGTCC AGCTGCTGGC GCTCTTCTTC
    CACAGATCCA TACTAAAGTG TCATGGTCTC TGGGACGAGG ACGACCCCAA AGTGCCCCGT
    AAGGATGAGT GTCCTCGTCA GGAGTCTGTG GATGACGGGA AGCTGTCGGT GGTCATGTCA
    CCTGATGAAG ACAGGAAGAG TTCTCCGGCG TATTCACTCA AGTCTATGAA TCTGGGAATG
    TCTGTGGACT CATCGCAGGT TCACGTACAG ATGCGTTATC CAGAGCAGCG GCCGTACCTG
    CGCAGACAGA GCACCAGCGG ATCACACTTC TCCCACAGAT CCTCCGCACG CTCCAAACGC
    GGCAGCACAA GCACACGTCA CAGCACCCAT GGGGAAAACA GCACAACAGA GTCAGAAGTA
    CCACAAAAGA GCCGGAAAGA AATGATTATG GAGAAAATCA GAGAGCAGCT CATCAAAGCA
    AAGGTTTACC TGCTCAAAAG ATTGGTTGAG TTTTATCAGC CCATCCGTCA GTTCTTCTAC
    AACCTGGTTC ATCCGGAGTA CAGCGCCGTG ACGGATGTTT ACGTATTGAT GTTTCTAGCA
    GACACTGTAG ATTTTATCAT CATCGTCTTT GGCTTTTGGG CATTTGGGAA ACATCAGGGT
    GGTGCTGACA TCACTTCCTC CCTGTCAGAG GACCAGGTTC CTGGGCCGTT CCTTGTTATG
    GTTCTCATAC AGTTTGGGAC GATGGTAGTG GATCGAGCCC TGTATCTGCG CAAGACTGTT
    GTGGGAAAAG TGATTTTTCA GGTCATCCTG GTGTTTGGCA TCCATTTCTG GATGTTTTTC
    ATCCTGCCGG GAATCACAGA GAGGCGCTTC AGTCAGAACA CTATCGCTCA GCTCTGGTAC
    TTTGTGAAGT GCATTTACTT CGGCCTGTCA GCCTATCAGA TCAGATGTGG ATATCCAACC
    CGCATCCTGG GAAACTTCCT CACCAAGAGC TACAACTACG CCAACCTCTT CCTCTTCCAG
    GGTTTCCGTC TGATCCCGTT CCTGACGGAG CTGCGTGCGG TGATGGACTG GGTCTGGACG
    GACACAACGC TCAGCTTGTC CAGCTGGATC TGTGTGGAGG ACATCTATGC TCACATCTTC
    ATCCTCAAAT GCTGGAGGGA GTCTGAGAAG CGTTACCCTC AGCCGCGCGG GCAGAAGAAG
    AAGAAGGTGG TGAAGTATGG GATGGGCGGC ATGATCGTGA TGCTCCTGAT CTGCATCGTC
    TGGTTTCCTC TGCTCTTCAT GTCTCTGGTC AAATCTGTGG CAGGAGTGGT TAACAAGCCT
    CTGGAGGTGT CCGTCTCGCT CACGCTCGGA GGACTCCAGC CGATCTTCAC AATGAGTGCC
    CAGCAGAACC ATATCAAATC CGTCACTGGG GCAGAATTTA AGAAGTTCAC AGATCACTAC
    AAGTCTAACG CAAATGCCAT CATGTTCCTG GAGTCGTATT TTCCTGAGGA TCTGACAATA
    GCACATCTAG AGGGAAGTTC AAACTCACTC TGGACCATCA GCCCCCCGAG CAAGGAAAAC
    CTGAAGAATA TGCTCAATGA CACGAGAGAG CCGTTTCCTC TCACCTTCAC CTGGTCCATT
    CAAAGGAACC TCAGTTTAGG TGCCAAAGCT GAAACTGCAA TGGGGAAATA CTTCATTGAG
    CTGGACGACG AGACTAGAAA AGGTCTAACT GATCAACTGG GGAATAGCAG CCAGCGTGTG
    ACAATCAAAA AAATATTTCC AAGGTACATC AGAGCACCAA GCGACTCCAA TGCCAAACCT
    ATTGACCAGC TTTACAACGA TGGAGAGTAC ATGGACATCA CACTGTTTTT AAATAAGAAG
    AACAGCTCAG AAGGCGTTCA GGAGTGGTGG ATCGTCAATC AGACAGATTC AGGTCCATTA
    CCTGGACTCA ACGGGTCTGG TCTTCAGATC TACATCATCA GTGACCAAGT CAGTCCACCC
    AGTCTGGGCT TTCTGGCTGG TTATGGGATC ATGGGTCTGT ACATGTCAGT GGTTTTGGTG
    ATCGGGAAGT TTGTGCGCGA GTTCTTCAGC GGGATCTCGC ACACCATCAT GTTCGAAGAG
    CTCCCTAACG TGGACCGAAT CCTCAAACTC TGCACCGATA TCTTCCTGGT CCGAGAGACG
    GGCGAACTGG ACCTGGAGGA AGACCTTTAC GCCAAACTCA TCTTCCTCTA CCGCTCTCCA
    GAAACCATGA TCAAATGGAC CAGAGAGAAG AGTGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.