PIEZO2 cDNA ORF clone, Gallus gallus(chicken)

The following PIEZO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIEZO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa146277 XM_025147819.1
Latest version!
Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa146277 XM_025147818.1
Latest version!
Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa146278 XM_015282541.2
Latest version!
Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa146279 XM_025147820.1
Latest version!
Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa146280 XM_025147821.1
Latest version!
Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa146281 XM_025147822.1
Latest version!
Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa146282 XM_025147824.1
Latest version!
Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa146283 XM_015282543.2
Latest version!
Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa32432 XM_015282541.1
Latest version!
Gallus gallus family with sequence similarity 38, member B (FAM38B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OGa32433 XM_015282542.1
Latest version!
Gallus gallus family with sequence similarity 38, member B (FAM38B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OGa32434 XM_015282543.1
Latest version!
Gallus gallus family with sequence similarity 38, member B (FAM38B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa146277
    Clone ID Related Accession (Same CDS sequence) XM_025147818.1 , XM_025147819.1
    Accession Version XM_025147818.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGCAGGAA GTACTGATGC AGACTGCGAA
    GACAGTGAAG AAGAACCTGT CAAGAAGAAG TCTGATGGCC CAGATAACAT CATCAAGAGG
    ATATTTAATA TTTTGAAGTT CACTTGGGTC CTTTTCCTGG CAACACTAGA CAGTTTTACA
    GCTTGGCTTA ATTCCATCTC AAGAGAACAC ATCGATATCT CTACTGTGCT GAGAATCGAA
    CGTTGTATGC TGACCAGGGA GATTAAGAAG GGAAACGTTC CTACCCGGGA GAGCATCCAT
    ATGTATTACC AGAACCATAT GATGAAACTT TCTAAGGAGT CAGGACTGGA CTCTATTGAT
    AAAAATCCTG GGCAAGCTTC TGGTTTGCAA ACATCTGAAA GAATGGATAG TTTAGATTCA
    GCAGCTTCTC GAGACAGTAT CTCCAGCTGC TACACTGAAG CAACCATGCT GTTCTCAAGG
    CAGTCAACCC TTGATGATTT AGATGGACCA GACACTGTTC CTAAAACAAG TGAGCGCGCT
    CGACCTAGGC TTCGTAAAAT GCAGAGCATG GATATGTCCT CTTCATCAGC TGACAGTGGC
    AGTATAGTGT CAAGCGAGCC TACACAGGTC ACCATGCTCT ATTCTCGTCA GGGGACGACA
    GAAACCATTG AGGAGGTAGA AGGAGAGCAT GAAGAGGAAG GAGCTCATTC TGCTTCAAGG
    CAGGATGAAG AAGAGGTGGA AGATTATAGC CTGGGTTCAG AAGGAGCTGC ATATACTCCC
    GACACCGATG TACCATTGTA CTCCACTGTG GACAGCAATG CAGAAGCACC ACCTTCCTAT
    AGCAAAGCTG TCAGCTTTGA ACACCTTCCT TTTGGCTCCC CAGATGACTC AGCTGGAAAG
    AGTCTCATGA TGGTGAGCCC AGATGACAGC CGGACGGACA GACTTAATGA TGCAATTCTC
    CCTCCATTGA CGCACGAGCT AACGGCTAGT GAGCTGCTTC TGAACAAGAT GTTTCATGAT
    GATGAGCTAG AGGAATCAGA GAAATTCTAT GTTGGTCAGC CTCGAGTCTT GCTTCTTATT
    TATGCGCTGT ACAATACTCT GGTGGCCCGG TCAGAAATGG TTTGCTATTT TGTCATCATC
    CTTAACCACA TGATCTCTGC CTCTATGATA ACGCTGGTGC TGCCCATCCT TATCTTTCTT
    TGGGCCATGC TGTCTGTCCC TCGACCAAGC AAACGTTTCT GGATGACTGC CATTGTCTAC
    ACTGAGGTAG CCATTGTCAT CAAGTACTTC TTCCAGTTTG GCTTCTTTCC TTGGAATAAA
    TATGTGGATT ACACAAAAGA TAAACCTTAT CATCCACCCA ATATTATTGG GATAGAGAAG
    AAAGAGGGCT ATGTGCACTA TGATCTTGTT CAGCTCCTTG CTTTATTTTT CCACAGATCC
    ATTTTAAAGT GCCATGGATT GTGGGATGAA GATGAGAAAG GGGATAACTC CAGTAATAAA
    GGAGATACTG ATGATGAGCT TTCTCTGCCG GGAGGCAGGA GAGACTCTTC TGGGTCTCTG
    AAGTCGGTCA ATCTTGCAGC ATCAGTGGAG TCCATCCATG TGCACTTCCC TGAGCAGCAG
    ACAGCAATCA GGAGGAAGAG CTCCAGCAGT GCATCACAGC TTTCTCACAG ATCCAGCTTC
    TCCTCACACA GATCTAAGAG AGGAAGCACC AGTACAAGGA ACAGCAGTCA GAAAGGGAGC
    AGTGTGCTAA GCATCAAGCA AAAATCTAGG AAAGAACTTC TTATGGAAAA GTTTCGAGAG
    CAAATGATCA AAGCCAAAGC CTTTACAATT AAAAAGACTC TCCAAGTGTA TGTGCCCATC
    AGACAATTTT TCTACAATCT TATTCATCCA GACTACAGTG CAGTGACTGA TGTATATGTG
    CTGATGTTCC TCGCAGATAC AGTGGACTTC ATCATCATTG TCTTTGGATT TTGGGCTTTT
    GGGAAACACT CTGCAGCAGC AGATATTACC TCTTCATTAT CAGAGGACCA GGTTCCAGAG
    GCATTTTTGG TGATGGTGCT CATTCAGTTT GGTACCATGG TGGTAGACCG AGCGCTCTAC
    CTCAAAAAGA CAGTCATGGG AAAGGTCATC TTCCAGGTCA TCCTTGTTTT TGGGATTCAT
    TTCTGGATGT TCTTTATCTT GCCTGGAGTG ACAGAAAGAA AATTTAGCCA GAACACCGTT
    GCCCAGCTCT GGTATTTTGT GAAATGTGTT TATTTTGGCT TATCAGCATA TCAGATACGC
    TGCGGATATC CAACTCGTGT TCTTGGCAAC TTCCTCACAA AAAGTTATAA CTATGTGAAC
    CTGTTCTTAT TTCAAGGATT CCGCCTGGTT CCATTTTTGA CTGAGCTGAG AGCAGTAATG
    GACTGGGTTT GGACAGATAC CACTCTCAGT CTCTCCAGCT GGATCTGTGT TGAAGATATC
    TATGCTCATA TATTCATCCT GAAGTGCTGG CGAGAGTCAG AAAAGAGATA TCCCCAACCA
    AGAGGCCAGA AGAAAAAGAA GGTTGTGAAG TATGGAATGG GTGGCATGAT TATCGTCTTG
    CTGATTTGTA TTGTCTGGTT CCCACTGCTC TTCATGTCTT TAATCAAATC TGTTGCAGGC
    ATCACCAACA AGCCTCTAGA TGTATCAATC ACAATAACTC TAGGAGGTTA TCAGCCTATT
    TTTACAATGA GTGCTCAACA GAGTCAGCTC AAGGATTTGA ATCAGACTGG TTTCAGTGCA
    TTTCTGGGAA GCTATAGGGG TAACACTGCT GCTTTGCAGT TTTTAGAGGG CTATGGAAAA
    GAAGATATAA CCCTAGCAGA TCTAGAAGGG AATTCAAACT CCTTATGGAC CATCAGCCCT
    CCCAGTAGAG AGAAAATGAT ACAGGGACTG CTGGATTTTT CAGCTGAGTT CACTGTAGTA
    CTTTCATGGA GCATTCAAAG AAATCTCACT CTTGGTGCCA AAGCAGAAAT AGCATCAGAT
    AAACTTACCT TTGGCCTACC AGAGAAAACC AGAAGAGATA TTGCTACAAT GATGTCTGGA
    AAGCCATTGG AAAAGGTAAC ATTAGAAACA GTGTATCCAT ACTACATCAA GGCTCCTAGT
    GATTCTCTGG CAAAACCCAT AAAGCAGCTA TTGACAGACT GCAGATGGGA AAATATCACA
    GTTTCCCTGG TCAAAAATGT GTCTGAAGAG GGAGTTCGTG AGTGGTGGGT TTTGAATCAG
    CTTGGAAAAA GATACAAAAC AAATGAAGAG AGCCTTGAAC TCTTCATATT CAGTGATAAA
    GTCAGTCCTC CAAGCCTTGG ATTCTTGGCT GGATATGGCA TCATGGGACT ATACGCGTCA
    GTCGTCCTGG TGATAGGGAA GTTCGTCCGT GAGTTTTTCA GTGGGATTTC TCACTCCATC
    ATGTTTGAGG AGCTACCCAA TGTAGACCGA ATCTTGAAGT TGTGTACTGA CATTTTCTTA
    GTACGAGAGA CTGGAGAACT GGAACTAGAA GAAGACCTGT ACGCCAAACT AATCTTCCTG
    TATCGCTCAC CAGAGACTAT GATCAAGTGG ACTAGGGAAA AAACTAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003586.1
    CDS848..9238
    Translation

    Target ORF information:

    RefSeq Version XM_025147818.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147818.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGCAGGAA GTACTGATGC AGACTGCGAA
    GACAGTGAAG AAGAACCTGT CAAGAAGAAG TCTGATGGCC CAGATAACAT CATCAAGAGG
    ATATTTAATA TTTTGAAGTT CACTTGGGTC CTTTTCCTGG CAACACTAGA CAGTTTTACA
    GCTTGGCTTA ATTCCATCTC AAGAGAACAC ATCGATATCT CTACTGTGCT GAGAATCGAA
    CGTTGTATGC TGACCAGGGA GATTAAGAAG GGAAACGTTC CTACCCGGGA GAGCATCCAT
    ATGTATTACC AGAACCATAT GATGAAACTT TCTAAGGAGT CAGGACTGGA CTCTATTGAT
    AAAAATCCTG GGCAAGCTTC TGGTTTGCAA ACATCTGAAA GAATGGATAG TTTAGATTCA
    GCAGCTTCTC GAGACAGTAT CTCCAGCTGC TACACTGAAG CAACCATGCT GTTCTCAAGG
    CAGTCAACCC TTGATGATTT AGATGGACCA GACACTGTTC CTAAAACAAG TGAGCGCGCT
    CGACCTAGGC TTCGTAAAAT GCAGAGCATG GATATGTCCT CTTCATCAGC TGACAGTGGC
    AGTATAGTGT CAAGCGAGCC TACACAGGTC ACCATGCTCT ATTCTCGTCA GGGGACGACA
    GAAACCATTG AGGAGGTAGA AGGAGAGCAT GAAGAGGAAG GAGCTCATTC TGCTTCAAGG
    CAGGATGAAG AAGAGGTGGA AGATTATAGC CTGGGTTCAG AAGGAGCTGC ATATACTCCC
    GACACCGATG TACCATTGTA CTCCACTGTG GACAGCAATG CAGAAGCACC ACCTTCCTAT
    AGCAAAGCTG TCAGCTTTGA ACACCTTCCT TTTGGCTCCC CAGATGACTC AGCTGGAAAG
    AGTCTCATGA TGGTGAGCCC AGATGACAGC CGGACGGACA GACTTAATGA TGCAATTCTC
    CCTCCATTGA CGCACGAGCT AACGGCTAGT GAGCTGCTTC TGAACAAGAT GTTTCATGAT
    GATGAGCTAG AGGAATCAGA GAAATTCTAT GTTGGTCAGC CTCGAGTCTT GCTTCTTATT
    TATGCGCTGT ACAATACTCT GGTGGCCCGG TCAGAAATGG TTTGCTATTT TGTCATCATC
    CTTAACCACA TGATCTCTGC CTCTATGATA ACGCTGGTGC TGCCCATCCT TATCTTTCTT
    TGGGCCATGC TGTCTGTCCC TCGACCAAGC AAACGTTTCT GGATGACTGC CATTGTCTAC
    ACTGAGGTAG CCATTGTCAT CAAGTACTTC TTCCAGTTTG GCTTCTTTCC TTGGAATAAA
    TATGTGGATT ACACAAAAGA TAAACCTTAT CATCCACCCA ATATTATTGG GATAGAGAAG
    AAAGAGGGCT ATGTGCACTA TGATCTTGTT CAGCTCCTTG CTTTATTTTT CCACAGATCC
    ATTTTAAAGT GCCATGGATT GTGGGATGAA GATGAGAAAG GGGATAACTC CAGTAATAAA
    GGAGATACTG ATGATGAGCT TTCTCTGCCG GGAGGCAGGA GAGACTCTTC TGGGTCTCTG
    AAGTCGGTCA ATCTTGCAGC ATCAGTGGAG TCCATCCATG TGCACTTCCC TGAGCAGCAG
    ACAGCAATCA GGAGGAAGAG CTCCAGCAGT GCATCACAGC TTTCTCACAG ATCCAGCTTC
    TCCTCACACA GATCTAAGAG AGGAAGCACC AGTACAAGGA ACAGCAGTCA GAAAGGGAGC
    AGTGTGCTAA GCATCAAGCA AAAATCTAGG AAAGAACTTC TTATGGAAAA GTTTCGAGAG
    CAAATGATCA AAGCCAAAGC CTTTACAATT AAAAAGACTC TCCAAGTGTA TGTGCCCATC
    AGACAATTTT TCTACAATCT TATTCATCCA GACTACAGTG CAGTGACTGA TGTATATGTG
    CTGATGTTCC TCGCAGATAC AGTGGACTTC ATCATCATTG TCTTTGGATT TTGGGCTTTT
    GGGAAACACT CTGCAGCAGC AGATATTACC TCTTCATTAT CAGAGGACCA GGTTCCAGAG
    GCATTTTTGG TGATGGTGCT CATTCAGTTT GGTACCATGG TGGTAGACCG AGCGCTCTAC
    CTCAAAAAGA CAGTCATGGG AAAGGTCATC TTCCAGGTCA TCCTTGTTTT TGGGATTCAT
    TTCTGGATGT TCTTTATCTT GCCTGGAGTG ACAGAAAGAA AATTTAGCCA GAACACCGTT
    GCCCAGCTCT GGTATTTTGT GAAATGTGTT TATTTTGGCT TATCAGCATA TCAGATACGC
    TGCGGATATC CAACTCGTGT TCTTGGCAAC TTCCTCACAA AAAGTTATAA CTATGTGAAC
    CTGTTCTTAT TTCAAGGATT CCGCCTGGTT CCATTTTTGA CTGAGCTGAG AGCAGTAATG
    GACTGGGTTT GGACAGATAC CACTCTCAGT CTCTCCAGCT GGATCTGTGT TGAAGATATC
    TATGCTCATA TATTCATCCT GAAGTGCTGG CGAGAGTCAG AAAAGAGATA TCCCCAACCA
    AGAGGCCAGA AGAAAAAGAA GGTTGTGAAG TATGGAATGG GTGGCATGAT TATCGTCTTG
    CTGATTTGTA TTGTCTGGTT CCCACTGCTC TTCATGTCTT TAATCAAATC TGTTGCAGGC
    ATCACCAACA AGCCTCTAGA TGTATCAATC ACAATAACTC TAGGAGGTTA TCAGCCTATT
    TTTACAATGA GTGCTCAACA GAGTCAGCTC AAGGATTTGA ATCAGACTGG TTTCAGTGCA
    TTTCTGGGAA GCTATAGGGG TAACACTGCT GCTTTGCAGT TTTTAGAGGG CTATGGAAAA
    GAAGATATAA CCCTAGCAGA TCTAGAAGGG AATTCAAACT CCTTATGGAC CATCAGCCCT
    CCCAGTAGAG AGAAAATGAT ACAGGGACTG CTGGATTTTT CAGCTGAGTT CACTGTAGTA
    CTTTCATGGA GCATTCAAAG AAATCTCACT CTTGGTGCCA AAGCAGAAAT AGCATCAGAT
    AAACTTACCT TTGGCCTACC AGAGAAAACC AGAAGAGATA TTGCTACAAT GATGTCTGGA
    AAGCCATTGG AAAAGGTAAC ATTAGAAACA GTGTATCCAT ACTACATCAA GGCTCCTAGT
    GATTCTCTGG CAAAACCCAT AAAGCAGCTA TTGACAGACT GCAGATGGGA AAATATCACA
    GTTTCCCTGG TCAAAAATGT GTCTGAAGAG GGAGTTCGTG AGTGGTGGGT TTTGAATCAG
    CTTGGAAAAA GATACAAAAC AAATGAAGAG AGCCTTGAAC TCTTCATATT CAGTGATAAA
    GTCAGTCCTC CAAGCCTTGG ATTCTTGGCT GGATATGGCA TCATGGGACT ATACGCGTCA
    GTCGTCCTGG TGATAGGGAA GTTCGTCCGT GAGTTTTTCA GTGGGATTTC TCACTCCATC
    ATGTTTGAGG AGCTACCCAA TGTAGACCGA ATCTTGAAGT TGTGTACTGA CATTTTCTTA
    GTACGAGAGA CTGGAGAACT GGAACTAGAA GAAGACCTGT ACGCCAAACT AATCTTCCTG
    TATCGCTCAC CAGAGACTAT GATCAAGTGG ACTAGGGAAA AAACTAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146277
    Clone ID Related Accession (Same CDS sequence) XM_025147818.1 , XM_025147819.1
    Accession Version XM_025147819.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGCAGGAA GTACTGATGC AGACTGCGAA
    GACAGTGAAG AAGAACCTGT CAAGAAGAAG TCTGATGGCC CAGATAACAT CATCAAGAGG
    ATATTTAATA TTTTGAAGTT CACTTGGGTC CTTTTCCTGG CAACACTAGA CAGTTTTACA
    GCTTGGCTTA ATTCCATCTC AAGAGAACAC ATCGATATCT CTACTGTGCT GAGAATCGAA
    CGTTGTATGC TGACCAGGGA GATTAAGAAG GGAAACGTTC CTACCCGGGA GAGCATCCAT
    ATGTATTACC AGAACCATAT GATGAAACTT TCTAAGGAGT CAGGACTGGA CTCTATTGAT
    AAAAATCCTG GGCAAGCTTC TGGTTTGCAA ACATCTGAAA GAATGGATAG TTTAGATTCA
    GCAGCTTCTC GAGACAGTAT CTCCAGCTGC TACACTGAAG CAACCATGCT GTTCTCAAGG
    CAGTCAACCC TTGATGATTT AGATGGACCA GACACTGTTC CTAAAACAAG TGAGCGCGCT
    CGACCTAGGC TTCGTAAAAT GCAGAGCATG GATATGTCCT CTTCATCAGC TGACAGTGGC
    AGTATAGTGT CAAGCGAGCC TACACAGGTC ACCATGCTCT ATTCTCGTCA GGGGACGACA
    GAAACCATTG AGGAGGTAGA AGGAGAGCAT GAAGAGGAAG GAGCTCATTC TGCTTCAAGG
    CAGGATGAAG AAGAGGTGGA AGATTATAGC CTGGGTTCAG AAGGAGCTGC ATATACTCCC
    GACACCGATG TACCATTGTA CTCCACTGTG GACAGCAATG CAGAAGCACC ACCTTCCTAT
    AGCAAAGCTG TCAGCTTTGA ACACCTTCCT TTTGGCTCCC CAGATGACTC AGCTGGAAAG
    AGTCTCATGA TGGTGAGCCC AGATGACAGC CGGACGGACA GACTTAATGA TGCAATTCTC
    CCTCCATTGA CGCACGAGCT AACGGCTAGT GAGCTGCTTC TGAACAAGAT GTTTCATGAT
    GATGAGCTAG AGGAATCAGA GAAATTCTAT GTTGGTCAGC CTCGAGTCTT GCTTCTTATT
    TATGCGCTGT ACAATACTCT GGTGGCCCGG TCAGAAATGG TTTGCTATTT TGTCATCATC
    CTTAACCACA TGATCTCTGC CTCTATGATA ACGCTGGTGC TGCCCATCCT TATCTTTCTT
    TGGGCCATGC TGTCTGTCCC TCGACCAAGC AAACGTTTCT GGATGACTGC CATTGTCTAC
    ACTGAGGTAG CCATTGTCAT CAAGTACTTC TTCCAGTTTG GCTTCTTTCC TTGGAATAAA
    TATGTGGATT ACACAAAAGA TAAACCTTAT CATCCACCCA ATATTATTGG GATAGAGAAG
    AAAGAGGGCT ATGTGCACTA TGATCTTGTT CAGCTCCTTG CTTTATTTTT CCACAGATCC
    ATTTTAAAGT GCCATGGATT GTGGGATGAA GATGAGAAAG GGGATAACTC CAGTAATAAA
    GGAGATACTG ATGATGAGCT TTCTCTGCCG GGAGGCAGGA GAGACTCTTC TGGGTCTCTG
    AAGTCGGTCA ATCTTGCAGC ATCAGTGGAG TCCATCCATG TGCACTTCCC TGAGCAGCAG
    ACAGCAATCA GGAGGAAGAG CTCCAGCAGT GCATCACAGC TTTCTCACAG ATCCAGCTTC
    TCCTCACACA GATCTAAGAG AGGAAGCACC AGTACAAGGA ACAGCAGTCA GAAAGGGAGC
    AGTGTGCTAA GCATCAAGCA AAAATCTAGG AAAGAACTTC TTATGGAAAA GTTTCGAGAG
    CAAATGATCA AAGCCAAAGC CTTTACAATT AAAAAGACTC TCCAAGTGTA TGTGCCCATC
    AGACAATTTT TCTACAATCT TATTCATCCA GACTACAGTG CAGTGACTGA TGTATATGTG
    CTGATGTTCC TCGCAGATAC AGTGGACTTC ATCATCATTG TCTTTGGATT TTGGGCTTTT
    GGGAAACACT CTGCAGCAGC AGATATTACC TCTTCATTAT CAGAGGACCA GGTTCCAGAG
    GCATTTTTGG TGATGGTGCT CATTCAGTTT GGTACCATGG TGGTAGACCG AGCGCTCTAC
    CTCAAAAAGA CAGTCATGGG AAAGGTCATC TTCCAGGTCA TCCTTGTTTT TGGGATTCAT
    TTCTGGATGT TCTTTATCTT GCCTGGAGTG ACAGAAAGAA AATTTAGCCA GAACACCGTT
    GCCCAGCTCT GGTATTTTGT GAAATGTGTT TATTTTGGCT TATCAGCATA TCAGATACGC
    TGCGGATATC CAACTCGTGT TCTTGGCAAC TTCCTCACAA AAAGTTATAA CTATGTGAAC
    CTGTTCTTAT TTCAAGGATT CCGCCTGGTT CCATTTTTGA CTGAGCTGAG AGCAGTAATG
    GACTGGGTTT GGACAGATAC CACTCTCAGT CTCTCCAGCT GGATCTGTGT TGAAGATATC
    TATGCTCATA TATTCATCCT GAAGTGCTGG CGAGAGTCAG AAAAGAGATA TCCCCAACCA
    AGAGGCCAGA AGAAAAAGAA GGTTGTGAAG TATGGAATGG GTGGCATGAT TATCGTCTTG
    CTGATTTGTA TTGTCTGGTT CCCACTGCTC TTCATGTCTT TAATCAAATC TGTTGCAGGC
    ATCACCAACA AGCCTCTAGA TGTATCAATC ACAATAACTC TAGGAGGTTA TCAGCCTATT
    TTTACAATGA GTGCTCAACA GAGTCAGCTC AAGGATTTGA ATCAGACTGG TTTCAGTGCA
    TTTCTGGGAA GCTATAGGGG TAACACTGCT GCTTTGCAGT TTTTAGAGGG CTATGGAAAA
    GAAGATATAA CCCTAGCAGA TCTAGAAGGG AATTCAAACT CCTTATGGAC CATCAGCCCT
    CCCAGTAGAG AGAAAATGAT ACAGGGACTG CTGGATTTTT CAGCTGAGTT CACTGTAGTA
    CTTTCATGGA GCATTCAAAG AAATCTCACT CTTGGTGCCA AAGCAGAAAT AGCATCAGAT
    AAACTTACCT TTGGCCTACC AGAGAAAACC AGAAGAGATA TTGCTACAAT GATGTCTGGA
    AAGCCATTGG AAAAGGTAAC ATTAGAAACA GTGTATCCAT ACTACATCAA GGCTCCTAGT
    GATTCTCTGG CAAAACCCAT AAAGCAGCTA TTGACAGACT GCAGATGGGA AAATATCACA
    GTTTCCCTGG TCAAAAATGT GTCTGAAGAG GGAGTTCGTG AGTGGTGGGT TTTGAATCAG
    CTTGGAAAAA GATACAAAAC AAATGAAGAG AGCCTTGAAC TCTTCATATT CAGTGATAAA
    GTCAGTCCTC CAAGCCTTGG ATTCTTGGCT GGATATGGCA TCATGGGACT ATACGCGTCA
    GTCGTCCTGG TGATAGGGAA GTTCGTCCGT GAGTTTTTCA GTGGGATTTC TCACTCCATC
    ATGTTTGAGG AGCTACCCAA TGTAGACCGA ATCTTGAAGT TGTGTACTGA CATTTTCTTA
    GTACGAGAGA CTGGAGAACT GGAACTAGAA GAAGACCTGT ACGCCAAACT AATCTTCCTG
    TATCGCTCAC CAGAGACTAT GATCAAGTGG ACTAGGGAAA AAACTAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003587.1
    CDS736..9126
    Translation

    Target ORF information:

    RefSeq Version XM_025147819.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147819.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGCAGGAA GTACTGATGC AGACTGCGAA
    GACAGTGAAG AAGAACCTGT CAAGAAGAAG TCTGATGGCC CAGATAACAT CATCAAGAGG
    ATATTTAATA TTTTGAAGTT CACTTGGGTC CTTTTCCTGG CAACACTAGA CAGTTTTACA
    GCTTGGCTTA ATTCCATCTC AAGAGAACAC ATCGATATCT CTACTGTGCT GAGAATCGAA
    CGTTGTATGC TGACCAGGGA GATTAAGAAG GGAAACGTTC CTACCCGGGA GAGCATCCAT
    ATGTATTACC AGAACCATAT GATGAAACTT TCTAAGGAGT CAGGACTGGA CTCTATTGAT
    AAAAATCCTG GGCAAGCTTC TGGTTTGCAA ACATCTGAAA GAATGGATAG TTTAGATTCA
    GCAGCTTCTC GAGACAGTAT CTCCAGCTGC TACACTGAAG CAACCATGCT GTTCTCAAGG
    CAGTCAACCC TTGATGATTT AGATGGACCA GACACTGTTC CTAAAACAAG TGAGCGCGCT
    CGACCTAGGC TTCGTAAAAT GCAGAGCATG GATATGTCCT CTTCATCAGC TGACAGTGGC
    AGTATAGTGT CAAGCGAGCC TACACAGGTC ACCATGCTCT ATTCTCGTCA GGGGACGACA
    GAAACCATTG AGGAGGTAGA AGGAGAGCAT GAAGAGGAAG GAGCTCATTC TGCTTCAAGG
    CAGGATGAAG AAGAGGTGGA AGATTATAGC CTGGGTTCAG AAGGAGCTGC ATATACTCCC
    GACACCGATG TACCATTGTA CTCCACTGTG GACAGCAATG CAGAAGCACC ACCTTCCTAT
    AGCAAAGCTG TCAGCTTTGA ACACCTTCCT TTTGGCTCCC CAGATGACTC AGCTGGAAAG
    AGTCTCATGA TGGTGAGCCC AGATGACAGC CGGACGGACA GACTTAATGA TGCAATTCTC
    CCTCCATTGA CGCACGAGCT AACGGCTAGT GAGCTGCTTC TGAACAAGAT GTTTCATGAT
    GATGAGCTAG AGGAATCAGA GAAATTCTAT GTTGGTCAGC CTCGAGTCTT GCTTCTTATT
    TATGCGCTGT ACAATACTCT GGTGGCCCGG TCAGAAATGG TTTGCTATTT TGTCATCATC
    CTTAACCACA TGATCTCTGC CTCTATGATA ACGCTGGTGC TGCCCATCCT TATCTTTCTT
    TGGGCCATGC TGTCTGTCCC TCGACCAAGC AAACGTTTCT GGATGACTGC CATTGTCTAC
    ACTGAGGTAG CCATTGTCAT CAAGTACTTC TTCCAGTTTG GCTTCTTTCC TTGGAATAAA
    TATGTGGATT ACACAAAAGA TAAACCTTAT CATCCACCCA ATATTATTGG GATAGAGAAG
    AAAGAGGGCT ATGTGCACTA TGATCTTGTT CAGCTCCTTG CTTTATTTTT CCACAGATCC
    ATTTTAAAGT GCCATGGATT GTGGGATGAA GATGAGAAAG GGGATAACTC CAGTAATAAA
    GGAGATACTG ATGATGAGCT TTCTCTGCCG GGAGGCAGGA GAGACTCTTC TGGGTCTCTG
    AAGTCGGTCA ATCTTGCAGC ATCAGTGGAG TCCATCCATG TGCACTTCCC TGAGCAGCAG
    ACAGCAATCA GGAGGAAGAG CTCCAGCAGT GCATCACAGC TTTCTCACAG ATCCAGCTTC
    TCCTCACACA GATCTAAGAG AGGAAGCACC AGTACAAGGA ACAGCAGTCA GAAAGGGAGC
    AGTGTGCTAA GCATCAAGCA AAAATCTAGG AAAGAACTTC TTATGGAAAA GTTTCGAGAG
    CAAATGATCA AAGCCAAAGC CTTTACAATT AAAAAGACTC TCCAAGTGTA TGTGCCCATC
    AGACAATTTT TCTACAATCT TATTCATCCA GACTACAGTG CAGTGACTGA TGTATATGTG
    CTGATGTTCC TCGCAGATAC AGTGGACTTC ATCATCATTG TCTTTGGATT TTGGGCTTTT
    GGGAAACACT CTGCAGCAGC AGATATTACC TCTTCATTAT CAGAGGACCA GGTTCCAGAG
    GCATTTTTGG TGATGGTGCT CATTCAGTTT GGTACCATGG TGGTAGACCG AGCGCTCTAC
    CTCAAAAAGA CAGTCATGGG AAAGGTCATC TTCCAGGTCA TCCTTGTTTT TGGGATTCAT
    TTCTGGATGT TCTTTATCTT GCCTGGAGTG ACAGAAAGAA AATTTAGCCA GAACACCGTT
    GCCCAGCTCT GGTATTTTGT GAAATGTGTT TATTTTGGCT TATCAGCATA TCAGATACGC
    TGCGGATATC CAACTCGTGT TCTTGGCAAC TTCCTCACAA AAAGTTATAA CTATGTGAAC
    CTGTTCTTAT TTCAAGGATT CCGCCTGGTT CCATTTTTGA CTGAGCTGAG AGCAGTAATG
    GACTGGGTTT GGACAGATAC CACTCTCAGT CTCTCCAGCT GGATCTGTGT TGAAGATATC
    TATGCTCATA TATTCATCCT GAAGTGCTGG CGAGAGTCAG AAAAGAGATA TCCCCAACCA
    AGAGGCCAGA AGAAAAAGAA GGTTGTGAAG TATGGAATGG GTGGCATGAT TATCGTCTTG
    CTGATTTGTA TTGTCTGGTT CCCACTGCTC TTCATGTCTT TAATCAAATC TGTTGCAGGC
    ATCACCAACA AGCCTCTAGA TGTATCAATC ACAATAACTC TAGGAGGTTA TCAGCCTATT
    TTTACAATGA GTGCTCAACA GAGTCAGCTC AAGGATTTGA ATCAGACTGG TTTCAGTGCA
    TTTCTGGGAA GCTATAGGGG TAACACTGCT GCTTTGCAGT TTTTAGAGGG CTATGGAAAA
    GAAGATATAA CCCTAGCAGA TCTAGAAGGG AATTCAAACT CCTTATGGAC CATCAGCCCT
    CCCAGTAGAG AGAAAATGAT ACAGGGACTG CTGGATTTTT CAGCTGAGTT CACTGTAGTA
    CTTTCATGGA GCATTCAAAG AAATCTCACT CTTGGTGCCA AAGCAGAAAT AGCATCAGAT
    AAACTTACCT TTGGCCTACC AGAGAAAACC AGAAGAGATA TTGCTACAAT GATGTCTGGA
    AAGCCATTGG AAAAGGTAAC ATTAGAAACA GTGTATCCAT ACTACATCAA GGCTCCTAGT
    GATTCTCTGG CAAAACCCAT AAAGCAGCTA TTGACAGACT GCAGATGGGA AAATATCACA
    GTTTCCCTGG TCAAAAATGT GTCTGAAGAG GGAGTTCGTG AGTGGTGGGT TTTGAATCAG
    CTTGGAAAAA GATACAAAAC AAATGAAGAG AGCCTTGAAC TCTTCATATT CAGTGATAAA
    GTCAGTCCTC CAAGCCTTGG ATTCTTGGCT GGATATGGCA TCATGGGACT ATACGCGTCA
    GTCGTCCTGG TGATAGGGAA GTTCGTCCGT GAGTTTTTCA GTGGGATTTC TCACTCCATC
    ATGTTTGAGG AGCTACCCAA TGTAGACCGA ATCTTGAAGT TGTGTACTGA CATTTTCTTA
    GTACGAGAGA CTGGAGAACT GGAACTAGAA GAAGACCTGT ACGCCAAACT AATCTTCCTG
    TATCGCTCAC CAGAGACTAT GATCAAGTGG ACTAGGGAAA AAACTAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146278
    Clone ID Related Accession (Same CDS sequence) XM_015282541.2
    Accession Version XM_015282541.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGGAAGTA CTGATGCAGA CTGCGAAGAC
    AGTGAAGAAG AACCTGTCAA GAAGAAGTCT GATGGCCCAG ATAACATCAT CAAGAGGATA
    TTTAATATTT TGAAGTTCAC TTGGGTCCTT TTCCTGGCAA CACTAGACAG TTTTACAGCT
    TGGCTTAATT CCATCTCAAG AGAACACATC GATATCTCTA CTGTGCTGAG AATCGAACGT
    TGTATGCTGA CCAGGGAGAT TAAGAAGGGA AACGTTCCTA CCCGGGAGAG CATCCATATG
    TATTACCAGA ACCATATGAT GAAACTTTCT AAGGAGTCAG GACTGGACTC TATTGATAAA
    AATCCTGGGC AAGCTTCTGG TTTGCAAACA TCTGAAAGAA TGGATAGTTT AGATTCAGCA
    GCTTCTCGAG ACAGTATCTC CAGCTGCTAC ACTGAAGCAA CCATGCTGTT CTCAAGGCAG
    TCAACCCTTG ATGATTTAGA TGGACCAGAC ACTGTTCCTA AAACAAGTGA GCGCGCTCGA
    CCTAGGCTTC GTAAAATGCA GAGCATGGAT ATGTCCTCTT CATCAGCTGA CAGTGGCAGT
    ATAGTGTCAA GCGAGCCTAC ACAGGTCACC ATGCTCTATT CTCGTCAGGG GACGACAGAA
    ACCATTGAGG AGGTAGAAGG AGAGCATGAA GAGGAAGGAG CTCATTCTGC TTCAAGGCAG
    GATGAAGAAG AGGTGGAAGA TTATAGCCTG GGTTCAGAAG GAGCTGCATA TACTCCCGAC
    ACCGATGTAC CATTGTACTC CACTGTGGAC AGCAATGCAG AAGCACCACC TTCCTATAGC
    AAAGCTGTCA GCTTTGAACA CCTTCCTTTT GGCTCCCCAG ATGACTCAGC TGGAAAGAGT
    CTCATGATGG TGAGCCCAGA TGACAGCCGG ACGGACAGAC TTAATGATGC AATTCTCCCT
    CCATTGACGC ACGAGCTAAC GGCTAGTGAG CTGCTTCTGA ACAAGATGTT TCATGATGAT
    GAGCTAGAGG AATCAGAGAA ATTCTATGTT GGTCAGCCTC GAGTCTTGCT TCTTATTTAT
    GCGCTGTACA ATACTCTGGT GGCCCGGTCA GAAATGGTTT GCTATTTTGT CATCATCCTT
    AACCACATGA TCTCTGCCTC TATGATAACG CTGGTGCTGC CCATCCTTAT CTTTCTTTGG
    GCCATGCTGT CTGTCCCTCG ACCAAGCAAA CGTTTCTGGA TGACTGCCAT TGTCTACACT
    GAGGTAGCCA TTGTCATCAA GTACTTCTTC CAGTTTGGCT TCTTTCCTTG GAATAAATAT
    GTGGATTACA CAAAAGATAA ACCTTATCAT CCACCCAATA TTATTGGGAT AGAGAAGAAA
    GAGGGCTATG TGCACTATGA TCTTGTTCAG CTCCTTGCTT TATTTTTCCA CAGATCCATT
    TTAAAGTGCC ATGGATTGTG GGATGAAGAT GAGAAAGGGG ATAACTCCAG TAATAAAGGA
    GATACTGATG ATGAGCTTTC TCTGCCGGGA GGCAGGAGAG ACTCTTCTGG GTCTCTGAAG
    TCGGTCAATC TTGCAGCATC AGTGGAGTCC ATCCATGTGC ACTTCCCTGA GCAGCAGACA
    GCAATCAGGA GGAAGAGCTC CAGCAGTGCA TCACAGCTTT CTCACAGATC CAGCTTCTCC
    TCACACAGAT CTAAGAGAGG AAGCACCAGT ACAAGGAACA GCAGTCAGAA AGGGAGCAGT
    GTGCTAAGCA TCAAGCAAAA ATCTAGGAAA GAACTTCTTA TGGAAAAGTT TCGAGAGCAA
    ATGATCAAAG CCAAAGCCTT TACAATTAAA AAGACTCTCC AAGTGTATGT GCCCATCAGA
    CAATTTTTCT ACAATCTTAT TCATCCAGAC TACAGTGCAG TGACTGATGT ATATGTGCTG
    ATGTTCCTCG CAGATACAGT GGACTTCATC ATCATTGTCT TTGGATTTTG GGCTTTTGGG
    AAACACTCTG CAGCAGCAGA TATTACCTCT TCATTATCAG AGGACCAGGT TCCAGAGGCA
    TTTTTGGTGA TGGTGCTCAT TCAGTTTGGT ACCATGGTGG TAGACCGAGC GCTCTACCTC
    AAAAAGACAG TCATGGGAAA GGTCATCTTC CAGGTCATCC TTGTTTTTGG GATTCATTTC
    TGGATGTTCT TTATCTTGCC TGGAGTGACA GAAAGAAAAT TTAGCCAGAA CACCGTTGCC
    CAGCTCTGGT ATTTTGTGAA ATGTGTTTAT TTTGGCTTAT CAGCATATCA GATACGCTGC
    GGATATCCAA CTCGTGTTCT TGGCAACTTC CTCACAAAAA GTTATAACTA TGTGAACCTG
    TTCTTATTTC AAGGATTCCG CCTGGTTCCA TTTTTGACTG AGCTGAGAGC AGTAATGGAC
    TGGGTTTGGA CAGATACCAC TCTCAGTCTC TCCAGCTGGA TCTGTGTTGA AGATATCTAT
    GCTCATATAT TCATCCTGAA GTGCTGGCGA GAGTCAGAAA AGAGATATCC CCAACCAAGA
    GGCCAGAAGA AAAAGAAGGT TGTGAAGTAT GGAATGGGTG GCATGATTAT CGTCTTGCTG
    ATTTGTATTG TCTGGTTCCC ACTGCTCTTC ATGTCTTTAA TCAAATCTGT TGCAGGCATC
    ACCAACAAGC CTCTAGATGT ATCAATCACA ATAACTCTAG GAGGTTATCA GCCTATTTTT
    ACAATGAGTG CTCAACAGAG TCAGCTCAAG GATTTGAATC AGACTGGTTT CAGTGCATTT
    CTGGGAAGCT ATAGGGGTAA CACTGCTGCT TTGCAGTTTT TAGAGGGCTA TGGAAAAGAA
    GATATAACCC TAGCAGATCT AGAAGGGAAT TCAAACTCCT TATGGACCAT CAGCCCTCCC
    AGTAGAGAGA AAATGATACA GGGACTGCTG GATTTTTCAG CTGAGTTCAC TGTAGTACTT
    TCATGGAGCA TTCAAAGAAA TCTCACTCTT GGTGCCAAAG CAGAAATAGC ATCAGATAAA
    CTTACCTTTG GCCTACCAGA GAAAACCAGA AGAGATATTG CTACAATGAT GTCTGGAAAG
    CCATTGGAAA AGGTAACATT AGAAACAGTG TATCCATACT ACATCAAGGC TCCTAGTGAT
    TCTCTGGCAA AACCCATAAA GCAGCTATTG ACAGACTGCA GATGGGAAAA TATCACAGTT
    TCCCTGGTCA AAAATGTGTC TGAAGAGGGA GTTCGTGAGT GGTGGGTTTT GAATCAGCTT
    GGAAAAAGAT ACAAAACAAA TGAAGAGAGC CTTGAACTCT TCATATTCAG TGATAAAGTC
    AGTCCTCCAA GCCTTGGATT CTTGGCTGGA TATGGCATCA TGGGACTATA CGCGTCAGTC
    GTCCTGGTGA TAGGGAAGTT CGTCCGTGAG TTTTTCAGTG GGATTTCTCA CTCCATCATG
    TTTGAGGAGC TACCCAATGT AGACCGAATC TTGAAGTTGT GTACTGACAT TTTCTTAGTA
    CGAGAGACTG GAGAACTGGA ACTAGAAGAA GACCTGTACG CCAAACTAAT CTTCCTGTAT
    CGCTCACCAG AGACTATGAT CAAGTGGACT AGGGAAAAAA CTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138027.2
    CDS848..9235
    Translation

    Target ORF information:

    RefSeq Version XM_015282541.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282541.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGGAAGTA CTGATGCAGA CTGCGAAGAC
    AGTGAAGAAG AACCTGTCAA GAAGAAGTCT GATGGCCCAG ATAACATCAT CAAGAGGATA
    TTTAATATTT TGAAGTTCAC TTGGGTCCTT TTCCTGGCAA CACTAGACAG TTTTACAGCT
    TGGCTTAATT CCATCTCAAG AGAACACATC GATATCTCTA CTGTGCTGAG AATCGAACGT
    TGTATGCTGA CCAGGGAGAT TAAGAAGGGA AACGTTCCTA CCCGGGAGAG CATCCATATG
    TATTACCAGA ACCATATGAT GAAACTTTCT AAGGAGTCAG GACTGGACTC TATTGATAAA
    AATCCTGGGC AAGCTTCTGG TTTGCAAACA TCTGAAAGAA TGGATAGTTT AGATTCAGCA
    GCTTCTCGAG ACAGTATCTC CAGCTGCTAC ACTGAAGCAA CCATGCTGTT CTCAAGGCAG
    TCAACCCTTG ATGATTTAGA TGGACCAGAC ACTGTTCCTA AAACAAGTGA GCGCGCTCGA
    CCTAGGCTTC GTAAAATGCA GAGCATGGAT ATGTCCTCTT CATCAGCTGA CAGTGGCAGT
    ATAGTGTCAA GCGAGCCTAC ACAGGTCACC ATGCTCTATT CTCGTCAGGG GACGACAGAA
    ACCATTGAGG AGGTAGAAGG AGAGCATGAA GAGGAAGGAG CTCATTCTGC TTCAAGGCAG
    GATGAAGAAG AGGTGGAAGA TTATAGCCTG GGTTCAGAAG GAGCTGCATA TACTCCCGAC
    ACCGATGTAC CATTGTACTC CACTGTGGAC AGCAATGCAG AAGCACCACC TTCCTATAGC
    AAAGCTGTCA GCTTTGAACA CCTTCCTTTT GGCTCCCCAG ATGACTCAGC TGGAAAGAGT
    CTCATGATGG TGAGCCCAGA TGACAGCCGG ACGGACAGAC TTAATGATGC AATTCTCCCT
    CCATTGACGC ACGAGCTAAC GGCTAGTGAG CTGCTTCTGA ACAAGATGTT TCATGATGAT
    GAGCTAGAGG AATCAGAGAA ATTCTATGTT GGTCAGCCTC GAGTCTTGCT TCTTATTTAT
    GCGCTGTACA ATACTCTGGT GGCCCGGTCA GAAATGGTTT GCTATTTTGT CATCATCCTT
    AACCACATGA TCTCTGCCTC TATGATAACG CTGGTGCTGC CCATCCTTAT CTTTCTTTGG
    GCCATGCTGT CTGTCCCTCG ACCAAGCAAA CGTTTCTGGA TGACTGCCAT TGTCTACACT
    GAGGTAGCCA TTGTCATCAA GTACTTCTTC CAGTTTGGCT TCTTTCCTTG GAATAAATAT
    GTGGATTACA CAAAAGATAA ACCTTATCAT CCACCCAATA TTATTGGGAT AGAGAAGAAA
    GAGGGCTATG TGCACTATGA TCTTGTTCAG CTCCTTGCTT TATTTTTCCA CAGATCCATT
    TTAAAGTGCC ATGGATTGTG GGATGAAGAT GAGAAAGGGG ATAACTCCAG TAATAAAGGA
    GATACTGATG ATGAGCTTTC TCTGCCGGGA GGCAGGAGAG ACTCTTCTGG GTCTCTGAAG
    TCGGTCAATC TTGCAGCATC AGTGGAGTCC ATCCATGTGC ACTTCCCTGA GCAGCAGACA
    GCAATCAGGA GGAAGAGCTC CAGCAGTGCA TCACAGCTTT CTCACAGATC CAGCTTCTCC
    TCACACAGAT CTAAGAGAGG AAGCACCAGT ACAAGGAACA GCAGTCAGAA AGGGAGCAGT
    GTGCTAAGCA TCAAGCAAAA ATCTAGGAAA GAACTTCTTA TGGAAAAGTT TCGAGAGCAA
    ATGATCAAAG CCAAAGCCTT TACAATTAAA AAGACTCTCC AAGTGTATGT GCCCATCAGA
    CAATTTTTCT ACAATCTTAT TCATCCAGAC TACAGTGCAG TGACTGATGT ATATGTGCTG
    ATGTTCCTCG CAGATACAGT GGACTTCATC ATCATTGTCT TTGGATTTTG GGCTTTTGGG
    AAACACTCTG CAGCAGCAGA TATTACCTCT TCATTATCAG AGGACCAGGT TCCAGAGGCA
    TTTTTGGTGA TGGTGCTCAT TCAGTTTGGT ACCATGGTGG TAGACCGAGC GCTCTACCTC
    AAAAAGACAG TCATGGGAAA GGTCATCTTC CAGGTCATCC TTGTTTTTGG GATTCATTTC
    TGGATGTTCT TTATCTTGCC TGGAGTGACA GAAAGAAAAT TTAGCCAGAA CACCGTTGCC
    CAGCTCTGGT ATTTTGTGAA ATGTGTTTAT TTTGGCTTAT CAGCATATCA GATACGCTGC
    GGATATCCAA CTCGTGTTCT TGGCAACTTC CTCACAAAAA GTTATAACTA TGTGAACCTG
    TTCTTATTTC AAGGATTCCG CCTGGTTCCA TTTTTGACTG AGCTGAGAGC AGTAATGGAC
    TGGGTTTGGA CAGATACCAC TCTCAGTCTC TCCAGCTGGA TCTGTGTTGA AGATATCTAT
    GCTCATATAT TCATCCTGAA GTGCTGGCGA GAGTCAGAAA AGAGATATCC CCAACCAAGA
    GGCCAGAAGA AAAAGAAGGT TGTGAAGTAT GGAATGGGTG GCATGATTAT CGTCTTGCTG
    ATTTGTATTG TCTGGTTCCC ACTGCTCTTC ATGTCTTTAA TCAAATCTGT TGCAGGCATC
    ACCAACAAGC CTCTAGATGT ATCAATCACA ATAACTCTAG GAGGTTATCA GCCTATTTTT
    ACAATGAGTG CTCAACAGAG TCAGCTCAAG GATTTGAATC AGACTGGTTT CAGTGCATTT
    CTGGGAAGCT ATAGGGGTAA CACTGCTGCT TTGCAGTTTT TAGAGGGCTA TGGAAAAGAA
    GATATAACCC TAGCAGATCT AGAAGGGAAT TCAAACTCCT TATGGACCAT CAGCCCTCCC
    AGTAGAGAGA AAATGATACA GGGACTGCTG GATTTTTCAG CTGAGTTCAC TGTAGTACTT
    TCATGGAGCA TTCAAAGAAA TCTCACTCTT GGTGCCAAAG CAGAAATAGC ATCAGATAAA
    CTTACCTTTG GCCTACCAGA GAAAACCAGA AGAGATATTG CTACAATGAT GTCTGGAAAG
    CCATTGGAAA AGGTAACATT AGAAACAGTG TATCCATACT ACATCAAGGC TCCTAGTGAT
    TCTCTGGCAA AACCCATAAA GCAGCTATTG ACAGACTGCA GATGGGAAAA TATCACAGTT
    TCCCTGGTCA AAAATGTGTC TGAAGAGGGA GTTCGTGAGT GGTGGGTTTT GAATCAGCTT
    GGAAAAAGAT ACAAAACAAA TGAAGAGAGC CTTGAACTCT TCATATTCAG TGATAAAGTC
    AGTCCTCCAA GCCTTGGATT CTTGGCTGGA TATGGCATCA TGGGACTATA CGCGTCAGTC
    GTCCTGGTGA TAGGGAAGTT CGTCCGTGAG TTTTTCAGTG GGATTTCTCA CTCCATCATG
    TTTGAGGAGC TACCCAATGT AGACCGAATC TTGAAGTTGT GTACTGACAT TTTCTTAGTA
    CGAGAGACTG GAGAACTGGA ACTAGAAGAA GACCTGTACG CCAAACTAAT CTTCCTGTAT
    CGCTCACCAG AGACTATGAT CAAGTGGACT AGGGAAAAAA CTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146279
    Clone ID Related Accession (Same CDS sequence) XM_025147820.1
    Accession Version XM_025147820.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTGTAC CTGAGGAAGA GAAATCTGAG AGCAGGGAAG ATGACAAAGA GATACCCTGC
    AGCCCAATTC CAATGACAGC AGAGAGAAGA CGCAGTCTGT GGTACGCAAG CCACTATACA
    ACTGATGAGA GAAAACTTCT GTCAATGACC CAAGATGAAT ACAAGCCATC GGATGTTCTG
    CTGGTAACAG TGAACGGGAA TCCTGCTGAC TATCACACCA TCCACCCCTC ACTGCCATTA
    GAGAATGGTC CAGCCAAAGC AGACATGTAC TCAACACCAC AGTACAAATG GGAGCCCTCG
    GATAACATGT CAGAAAAGGA AGAGGAGGAA GTAGAGGAGG AAGAAGAAGT TCCTCAGGAG
    GAAAAAGAAA ATGTTAAATT GCATGCCCTG GTCTCTGTGT TCCAATTTAT CATGAAGCAA
    AGTTACATTT GTGCTTTGAT AGCTATGATG GCATGGAGTA TCACGTATCA CAGCTGGTTG
    ACTTTTGTGC TCTTGATCTG GTCTTGCACG CTGTGGATGA TTCGAGACCG AAGGAAATAT
    GCTATGATCA GTTCACCGTT CATGGTTTTC TATGGAAATT TACTGCTAAT ATTACAATAT
    ATATGGAGTA TTGAGCTCAA GAATGATGAG CTTCCTCAAG TATCAGGTTT TTTAGAAAGA
    AAGGAACCTG GAGAGTTGGC ATCAAAGATT CTTTTCACGA TTACTTTCTG GCTACTGCTT
    AGGCAACACC TCACTGAACA GAAAGCACTT CAGTTAAAAG AAGCTACTTT ATCTGAGGTC
    AAAGTTGGAA ATGAAGAAGA TGAAGAAAAA GAAGAGGGAG AGTTGCAAGA GGGAGAAGTG
    GCAGAAGAAG AGGAGGAGGA GGAGGAGGAA CAGGAAGATG ATGATGAGGA TGAGCAGGAT
    ATTATGAAAG TTCTGGGGAA GCTGGTGATG GCTATGTTCA TCAAGTACTG GATTTATGTC
    TGTGGAGGCA TGTTCTTCTT CGTCAGCTTT GAGGGTAGAA TTGTCATGTA CAAAATCATC
    TACATGGTAC TATTCCTGTT CTGTGTGGCC CTCTATCAGG TACACTATGA ATGGTGGAGA
    AAGATTCTGA AATACTTTTG GATGTCAGTT GTTGTTTACA CGATGTTGGT ACTTATCTTC
    ATCTACACAT ACCAGTTTGA GTCATTCCCT GGGTTGTGGA AAAATATGAC AGGCCTGGAT
    GAAAACAAAC TAGCAGACCT TGGTTTAAAA AGATTTTCTG TGGCTGAGCT ATTCACTCGC
    ATCTTTATAC CCACCTCCTT CCTACTGGCG TGTATCTTGC ACCTTCACTA TTTCCATGAC
    CGTTTCCTCC AGCTCACTGA TTTAAAAGCT GTAACCAGCA AACAGGACAA CACTATTTAC
    AGACTGGTGC ATCAAGATGG AAGCCTGCCT GACATAACTA TGATGAATTT AACAGCCAGC
    AGTGAAAAGG AAGAGGATAA AATGCTGAAA GAGGCTGGTG AGAAAAGAAT GGAAGAGCCA
    GATGGGGAGG GAGGGAAAGG GAGGGCTGAA AAAGGAAAAG AAGAAGAAGA GGAAGATGAG
    GAGAAGGAAG ATGATGATGT AGACGATGAG TCAGAGGAAG AAGAAACTAC AGACCTAAGA
    AACAAGTGGC ATCTGGTGAT TGACCGCCTT ACAGTGCTGT TTTTGAAATT CCTGGAGTAC
    TTTCATAAGA TGCAAGTCTT TGTCTGGTGG CTTTTGGAGC TGCATATCAT CAAAATCGTC
    TCCTCTTACA TCATCTGGGT CACAGTAAAA GAGGTGTCTC TGTTTAACTT TGTGTTTTTA
    ATTGCTTGGG CTCTTGCTTT GCCATATGCT CAGTTTCGCC CATTGGCATC AAGTATCTGC
    ACTGTTTGGA CATGTGTGAT TATTGTCTGC AAAATGTTGT ACCAGCTCAC ATCTATTGAT
    CCCAGCACTT TTTCCAGTAA CTGTACATTG CCCAGAGAAA ATGAAACAAA GGTTGATTTA
    GAAGAGCTCA AAACATCAGT CCTCTACAGT GGTCCGGTTG ATCCTGCAGA ATGGGTTGGA
    TTGAGGAAGT CTTATCCCCT ACTTTTATAC TTAAGGAATA ACTTACTGAT GCTGGCTATT
    CTGGCTTTTG AAGTTACAAT TTACCGTCAT CAAGAGTACT ACAGATGTCG AAATAACCTT
    ACTGCACCTG TAACTAAAAC TATTTTCCAT GATATTACAA GAGCACATCT GGATGATGGA
    CTGGTAAACT GTGTCAAGTA TTTCATAAAC TACTTCTTCT ACAAGTTTGG TTTAGAGACC
    TGTTTTCTTT TATCAGTGAA TGTAATTGGT CAACGGATGG ATTTTTATGC CATGATTCAT
    GCCTTCTGGT TGATTGCTGT ATTGTATCGA CGTAGAAGAA AAGCTATTGC AGAGATCTGG
    CCAAAGTATT GCTGTTTTCT GGCATGCATC ATTACATTCC AGTATTTCCT TTGTATTGGC
    ATTCCACCTG CTCCTTGTAA AGACTATCCA TGGAGAAGTG GTAATGCCAA CTTCAACTCA
    AATATCATAA AGTGGTTATA CTTTCCAGAC TTCATTGTGA GACCAAACCC TGTCTTCCTT
    GTCTATGATT TCATGTTGCT GCTGTGTGCA TCCTTACAAA GGCAAACGTT TGAGGATGAA
    AATAAAGCTG CTGTGCGAAT CATGGCTGGA GACAACGTGG AAATTTGTAT GAATCTTGAA
    GCAGCATCTT TTAGCCAGCA TAATCCTGTT CCAGACTTCA TTCACTGCCG GTCCTACTTA
    GATATGTATA AGGTGATAAT CTTTAGTTAC CTCTTCTGGT TTGTGCTTAC TATAATCTTC
    ATAACGGGAA CAACGAGGAT CAGCATATTC TGTATGGGCT ACTTGGTTGC CTGCTTTTAT
    TTCCTTCTCT TTGGTGGAGA TCTGCTGCTG AAACCCATCA GGAGCATCCT GCGCTACTGG
    GACTGGCTGA TTGCATACAA TGTTTTTGTG ATCACCATGA AGAACATCCT CTCGATAGGA
    GCATGTGGCT ACATTGAGTC TTTAATTCAA AATAGTTGCT GGTTGATTCA GGCATTTAGT
    CTGGCTTGCA CAGTTAAAGG CTACCGTATC CCAACCAACA ATGCAGACTG TAAACTACCT
    AGTGGAGAAG CAGGAATCAT CTGGGACAGT ATATGTTTTG CCTTTCTACT GCTGCAAAGA
    AGAGTCTTCA TGAGTTACTA CTTCCTCCAC GTGGTTGCAG ATATAAAAGC TTCTCAGATC
    CTAGCATCAA GGGGTGCTGA GCTTTTCCAG GCCACAATTG TTAAGGCAGT AAAGGCAAGA
    ATTGAAGAGG AAAAAAAATC AATGGATCAA CTGAAAAGAC AAATGGATCG CATCAAAGCC
    AGGCAGCAAA AATATAAAAA GGGTAAAGAG AGGATGCTAA GCATGACACA GGATTCCACA
    GAGGGGCCTG AAATTCGGAA GGTTTCGGAA GAAGATGATG AAGGAGAAGC AGACAAAGAG
    AAAGCCAAGG GCAAAAAAAA GCTGTGGTGG CGGCCTTGGG TTGATCATGC TTCCATGGTC
    AGAAGTGGAA ATTATTATTT GTTTGAGACG GACAGTGAAG AGGAAGAGGA AGAAGAGAAA
    AAAGAAGAGG AAGAGCCTCG AAAAAAATCT GCATTTCAGT TTGTTTATCA GGCCTGGATT
    ACTGATCCTA AAACTGCGCT ACGACAGAGG CGCAAGGAAA AGAAAAGCTT TCAAAAAGAG
    GAGAAGCGAA GGCGTAAAGT ATATGGGGAT GCAGGAAGTA CTGATGCAGA CTGCGAAGAC
    AGTGAAGAAG AACCTGTCAA GAAGAAGTCT GATGGCCCAG ATAACATCAT CAAGAGGATA
    TTTAATATTT TGAAGTTCAC TTGGGTCCTT TTCCTGGCAA CACTAGACAG TTTTACAGCT
    TGGCTTAATT CCATCTCAAG AGAACACATC GATATCTCTA CTGTGCTGAG AATCGAACGT
    TGTATGCTGA CCAGGGAGAT TAAGAAGGGA AACGTTCCTA CCCGGGAGAG CATCCATATG
    TATTACCAGA ACCATATGAT GAAACTTTCT AAGGAGTCAG GACTGGACTC TATTGATAAA
    AATCCTGGGC AAGCTTCTGG TTTGCAAACA TCTGAAAGAA TGGATAGTTT AGATTCAGCA
    GCTTCTCGAG ACAGTATCTC CAGCTGCTAC ACTGAAGCAA CCATGCTGTT CTCAAGGCAG
    TCAACCCTTG ATGATTTAGA TGGACCAGAC ACTGTTCCTA AAACAAGTGA GCGCGCTCGA
    CCTAGGCTTC GTAAAATGCA GAGCATGGAT ATGTCCTCTT CATCAGCTGA CAGTGGCAGT
    ATAGTGTCAA GCGAGCCTAC ACAGGTCACC ATGCTCTATT CTCGTCAGGG GACGACAGAA
    ACCATTGAGG AGGTAGAAGG AGAGCATGAA GAGGAAGGAG CTCATTCTGC TTCAAGGCAG
    GATGAAGAAG AGGTGGAAGA TTATAGCCTG GGTTCAGAAG GAGCTGCATA TACTCCCGAC
    ACCGATGTAC CATTGTACTC CACTGTGGAC AGCAATGCAG AAGCACCACC TTCCTATAGC
    AAAGCTGTCA GCTTTGAACA CCTTCCTTTT GGCTCCCCAG ATGACTCAGC TGGAAAGAGT
    CTCATGATGG TGAGCCCAGA TGACAGCCGG ACGGACAGAC TTAATGATGC AATTCTCCCT
    CCATTGACGC ACGAGCTAAC GGCTAGTGAG CTGCTTCTGA ACAAGATGTT TCATGATGAT
    GAGCTAGAGG AATCAGAGAA ATTCTATGTT GGTCAGCCTC GAGTCTTGCT TCTTATTTAT
    GCGCTGTACA ATACTCTGGT GGCCCGGTCA GAAATGGTTT GCTATTTTGT CATCATCCTT
    AACCACATGA TCTCTGCCTC TATGATAACG CTGGTGCTGC CCATCCTTAT CTTTCTTTGG
    GCCATGCTGT CTGTCCCTCG ACCAAGCAAA CGTTTCTGGA TGACTGCCAT TGTCTACACT
    GAGGTAGCCA TTGTCATCAA GTACTTCTTC CAGTTTGGCT TCTTTCCTTG GAATAAATAT
    GTGGATTACA CAAAAGATAA ACCTTATCAT CCACCCAATA TTATTGGGAT AGAGAAGAAA
    GAGGGCTATG TGCACTATGA TCTTGTTCAG CTCCTTGCTT TATTTTTCCA CAGATCCATT
    TTAAAGTGCC ATGGATTGTG GGATGAAGAT GAGAAAGGGG ATAACTCCAG TAATAAAGGA
    GATACTGATG ATGAGCTTTC TCTGCCGGGA GGCAGGAGAG ACTCTTCTGG GTCTCTGAAG
    TCGGTCAATC TTGCAGCATC AGTGGAGTCC ATCCATGTGC ACTTCCCTGA GCAGCAGACA
    GCAATCAGGA GGAAGAGCTC CAGCAGTGCA TCACAGCTTT CTCACAGATC CAGCTTCTCC
    TCACACAGAT CTAAGAGAGG AAGCACCAGT ACAAGGAACA GCAGTCAGAA AGGGAGCAGT
    GTGCTAAGCA TCAAGCAAAA ATCTAGGAAA GAACTTCTTA TGGAAAAGTT TCGAGAGCAA
    ATGATCAAAG CCAAAGCCTT TACAATTAAA AAGACTCTCC AAGTGTATGT GCCCATCAGA
    CAATTTTTCT ACAATCTTAT TCATCCAGAC TACAGTGCAG TGACTGATGT ATATGTGCTG
    ATGTTCCTCG CAGATACAGT GGACTTCATC ATCATTGTCT TTGGATTTTG GGCTTTTGGG
    AAACACTCTG CAGCAGCAGA TATTACCTCT TCATTATCAG AGGACCAGGT TCCAGAGGCA
    TTTTTGGTGA TGGTGCTCAT TCAGTTTGGT ACCATGGTGG TAGACCGAGC GCTCTACCTC
    AAAAAGACAG TCATGGGAAA GGTCATCTTC CAGGTCATCC TTGTTTTTGG GATTCATTTC
    TGGATGTTCT TTATCTTGCC TGGAGTGACA GAAAGAAAAT TTAGCCAGAA CACCGTTGCC
    CAGCTCTGGT ATTTTGTGAA ATGTGTTTAT TTTGGCTTAT CAGCATATCA GATACGCTGC
    GGATATCCAA CTCGTGTTCT TGGCAACTTC CTCACAAAAA GTTATAACTA TGTGAACCTG
    TTCTTATTTC AAGGATTCCG CCTGGTTCCA TTTTTGACTG AGCTGAGAGC AGTAATGGAC
    TGGGTTTGGA CAGATACCAC TCTCAGTCTC TCCAGCTGGA TCTGTGTTGA AGATATCTAT
    GCTCATATAT TCATCCTGAA GTGCTGGCGA GAGTCAGAAA AGAGATATCC CCAACCAAGA
    GGCCAGAAGA AAAAGAAGGT TGTGAAGTAT GGAATGGGTG GCATGATTAT CGTCTTGCTG
    ATTTGTATTG TCTGGTTCCC ACTGCTCTTC ATGTCTTTAA TCAAATCTGT TGCAGGCATC
    ACCAACAAGC CTCTAGATGT ATCAATCACA ATAACTCTAG GAGGTTATCA GCCTATTTTT
    ACAATGAGTG CTCAACAGAG TCAGCTCAAG GATTTGAATC AGACTGGTTT CAGTGCATTT
    CTGGGAAGCT ATAGGGGTAA CACTGCTGCT TTGCAGTTTT TAGAGGGCTA TGGAAAAGAA
    GATATAACCC TAGCAGATCT AGAAGGGAAT TCAAACTCCT TATGGACCAT CAGCCCTCCC
    AGTAGAGAGA AAATGATACA GGGACTGCTG GATTTTTCAG CTGAGTTCAC TGTAGTACTT
    TCATGGAGCA TTCAAAGAAA TCTCACTCTT GGTGCCAAAG CAGAAATAGC ATCAGATAAA
    CTTACCTTTG GCCTACCAGA GAAAACCAGA AGAGATATTG CTACAATGAT GTCTGGAAAG
    CCATTGGAAA AGGTAACATT AGAAACAGTG TATCCATACT ACATCAAGGC TCCTAGTGAT
    TCTCTGGCAA AACCCATAAA GCAGCTATTG ACAGACTGCA GATGGGAAAA TATCACAGTT
    TCCCTGGTCA AAAATGTGTC TGAAGAGGGA GTTCGTGAGT GGTGGGTTTT GAATCAGCTT
    GGAAAAAGAT ACAAAACAAA TGAAGAGAGC CTTGAACTCT TCATATTCAG TGATAAAGTC
    AGTCCTCCAA GCCTTGGATT CTTGGCTGGA TATGGCATCA TGGGACTATA CGCGTCAGTC
    GTCCTGGTGA TAGGGAAGTT CGTCCGTGAG TTTTTCAGTG GGATTTCTCA CTCCATCATG
    TTTGAGGAGC TACCCAATGT AGACCGAATC TTGAAGTTGT GTACTGACAT TTTCTTAGTA
    CGAGAGACTG GAGAACTGGA ACTAGAAGAA GACCTGTACG CCAAACTAAT CTTCCTGTAT
    CGCTCACCAG AGACTATGAT CAAGTGGACT AGGGAAAAAA CTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003588.1
    CDS848..9235
    Translation

    Target ORF information:

    RefSeq Version XM_025147820.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147820.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTGTAC CTGAGGAAGA GAAATCTGAG AGCAGGGAAG ATGACAAAGA GATACCCTGC
    AGCCCAATTC CAATGACAGC AGAGAGAAGA CGCAGTCTGT GGTACGCAAG CCACTATACA
    ACTGATGAGA GAAAACTTCT GTCAATGACC CAAGATGAAT ACAAGCCATC GGATGTTCTG
    CTGGTAACAG TGAACGGGAA TCCTGCTGAC TATCACACCA TCCACCCCTC ACTGCCATTA
    GAGAATGGTC CAGCCAAAGC AGACATGTAC TCAACACCAC AGTACAAATG GGAGCCCTCG
    GATAACATGT CAGAAAAGGA AGAGGAGGAA GTAGAGGAGG AAGAAGAAGT TCCTCAGGAG
    GAAAAAGAAA ATGTTAAATT GCATGCCCTG GTCTCTGTGT TCCAATTTAT CATGAAGCAA
    AGTTACATTT GTGCTTTGAT AGCTATGATG GCATGGAGTA TCACGTATCA CAGCTGGTTG
    ACTTTTGTGC TCTTGATCTG GTCTTGCACG CTGTGGATGA TTCGAGACCG AAGGAAATAT
    GCTATGATCA GTTCACCGTT CATGGTTTTC TATGGAAATT TACTGCTAAT ATTACAATAT
    ATATGGAGTA TTGAGCTCAA GAATGATGAG CTTCCTCAAG TATCAGGTTT TTTAGAAAGA
    AAGGAACCTG GAGAGTTGGC ATCAAAGATT CTTTTCACGA TTACTTTCTG GCTACTGCTT
    AGGCAACACC TCACTGAACA GAAAGCACTT CAGTTAAAAG AAGCTACTTT ATCTGAGGTC
    AAAGTTGGAA ATGAAGAAGA TGAAGAAAAA GAAGAGGGAG AGTTGCAAGA GGGAGAAGTG
    GCAGAAGAAG AGGAGGAGGA GGAGGAGGAA CAGGAAGATG ATGATGAGGA TGAGCAGGAT
    ATTATGAAAG TTCTGGGGAA GCTGGTGATG GCTATGTTCA TCAAGTACTG GATTTATGTC
    TGTGGAGGCA TGTTCTTCTT CGTCAGCTTT GAGGGTAGAA TTGTCATGTA CAAAATCATC
    TACATGGTAC TATTCCTGTT CTGTGTGGCC CTCTATCAGG TACACTATGA ATGGTGGAGA
    AAGATTCTGA AATACTTTTG GATGTCAGTT GTTGTTTACA CGATGTTGGT ACTTATCTTC
    ATCTACACAT ACCAGTTTGA GTCATTCCCT GGGTTGTGGA AAAATATGAC AGGCCTGGAT
    GAAAACAAAC TAGCAGACCT TGGTTTAAAA AGATTTTCTG TGGCTGAGCT ATTCACTCGC
    ATCTTTATAC CCACCTCCTT CCTACTGGCG TGTATCTTGC ACCTTCACTA TTTCCATGAC
    CGTTTCCTCC AGCTCACTGA TTTAAAAGCT GTAACCAGCA AACAGGACAA CACTATTTAC
    AGACTGGTGC ATCAAGATGG AAGCCTGCCT GACATAACTA TGATGAATTT AACAGCCAGC
    AGTGAAAAGG AAGAGGATAA AATGCTGAAA GAGGCTGGTG AGAAAAGAAT GGAAGAGCCA
    GATGGGGAGG GAGGGAAAGG GAGGGCTGAA AAAGGAAAAG AAGAAGAAGA GGAAGATGAG
    GAGAAGGAAG ATGATGATGT AGACGATGAG TCAGAGGAAG AAGAAACTAC AGACCTAAGA
    AACAAGTGGC ATCTGGTGAT TGACCGCCTT ACAGTGCTGT TTTTGAAATT CCTGGAGTAC
    TTTCATAAGA TGCAAGTCTT TGTCTGGTGG CTTTTGGAGC TGCATATCAT CAAAATCGTC
    TCCTCTTACA TCATCTGGGT CACAGTAAAA GAGGTGTCTC TGTTTAACTT TGTGTTTTTA
    ATTGCTTGGG CTCTTGCTTT GCCATATGCT CAGTTTCGCC CATTGGCATC AAGTATCTGC
    ACTGTTTGGA CATGTGTGAT TATTGTCTGC AAAATGTTGT ACCAGCTCAC ATCTATTGAT
    CCCAGCACTT TTTCCAGTAA CTGTACATTG CCCAGAGAAA ATGAAACAAA GGTTGATTTA
    GAAGAGCTCA AAACATCAGT CCTCTACAGT GGTCCGGTTG ATCCTGCAGA ATGGGTTGGA
    TTGAGGAAGT CTTATCCCCT ACTTTTATAC TTAAGGAATA ACTTACTGAT GCTGGCTATT
    CTGGCTTTTG AAGTTACAAT TTACCGTCAT CAAGAGTACT ACAGATGTCG AAATAACCTT
    ACTGCACCTG TAACTAAAAC TATTTTCCAT GATATTACAA GAGCACATCT GGATGATGGA
    CTGGTAAACT GTGTCAAGTA TTTCATAAAC TACTTCTTCT ACAAGTTTGG TTTAGAGACC
    TGTTTTCTTT TATCAGTGAA TGTAATTGGT CAACGGATGG ATTTTTATGC CATGATTCAT
    GCCTTCTGGT TGATTGCTGT ATTGTATCGA CGTAGAAGAA AAGCTATTGC AGAGATCTGG
    CCAAAGTATT GCTGTTTTCT GGCATGCATC ATTACATTCC AGTATTTCCT TTGTATTGGC
    ATTCCACCTG CTCCTTGTAA AGACTATCCA TGGAGAAGTG GTAATGCCAA CTTCAACTCA
    AATATCATAA AGTGGTTATA CTTTCCAGAC TTCATTGTGA GACCAAACCC TGTCTTCCTT
    GTCTATGATT TCATGTTGCT GCTGTGTGCA TCCTTACAAA GGCAAACGTT TGAGGATGAA
    AATAAAGCTG CTGTGCGAAT CATGGCTGGA GACAACGTGG AAATTTGTAT GAATCTTGAA
    GCAGCATCTT TTAGCCAGCA TAATCCTGTT CCAGACTTCA TTCACTGCCG GTCCTACTTA
    GATATGTATA AGGTGATAAT CTTTAGTTAC CTCTTCTGGT TTGTGCTTAC TATAATCTTC
    ATAACGGGAA CAACGAGGAT CAGCATATTC TGTATGGGCT ACTTGGTTGC CTGCTTTTAT
    TTCCTTCTCT TTGGTGGAGA TCTGCTGCTG AAACCCATCA GGAGCATCCT GCGCTACTGG
    GACTGGCTGA TTGCATACAA TGTTTTTGTG ATCACCATGA AGAACATCCT CTCGATAGGA
    GCATGTGGCT ACATTGAGTC TTTAATTCAA AATAGTTGCT GGTTGATTCA GGCATTTAGT
    CTGGCTTGCA CAGTTAAAGG CTACCGTATC CCAACCAACA ATGCAGACTG TAAACTACCT
    AGTGGAGAAG CAGGAATCAT CTGGGACAGT ATATGTTTTG CCTTTCTACT GCTGCAAAGA
    AGAGTCTTCA TGAGTTACTA CTTCCTCCAC GTGGTTGCAG ATATAAAAGC TTCTCAGATC
    CTAGCATCAA GGGGTGCTGA GCTTTTCCAG GCCACAATTG TTAAGGCAGT AAAGGCAAGA
    ATTGAAGAGG AAAAAAAATC AATGGATCAA CTGAAAAGAC AAATGGATCG CATCAAAGCC
    AGGCAGCAAA AATATAAAAA GGGTAAAGAG AGGATGCTAA GCATGACACA GGATTCCACA
    GAGGGGCCTG AAATTCGGAA GGTTTCGGAA GAAGATGATG AAGGAGAAGC AGACAAAGAG
    AAAGCCAAGG GCAAAAAAAA GCTGTGGTGG CGGCCTTGGG TTGATCATGC TTCCATGGTC
    AGAAGTGGAA ATTATTATTT GTTTGAGACG GACAGTGAAG AGGAAGAGGA AGAAGAGAAA
    AAAGAAGAGG AAGAGCCTCG AAAAAAATCT GCATTTCAGT TTGTTTATCA GGCCTGGATT
    ACTGATCCTA AAACTGCGCT ACGACAGAGG CGCAAGGAAA AGAAAAGCTT TCAAAAAGAG
    GAGAAGCGAA GGCGTAAAGT ATATGGGGAT GCAGGAAGTA CTGATGCAGA CTGCGAAGAC
    AGTGAAGAAG AACCTGTCAA GAAGAAGTCT GATGGCCCAG ATAACATCAT CAAGAGGATA
    TTTAATATTT TGAAGTTCAC TTGGGTCCTT TTCCTGGCAA CACTAGACAG TTTTACAGCT
    TGGCTTAATT CCATCTCAAG AGAACACATC GATATCTCTA CTGTGCTGAG AATCGAACGT
    TGTATGCTGA CCAGGGAGAT TAAGAAGGGA AACGTTCCTA CCCGGGAGAG CATCCATATG
    TATTACCAGA ACCATATGAT GAAACTTTCT AAGGAGTCAG GACTGGACTC TATTGATAAA
    AATCCTGGGC AAGCTTCTGG TTTGCAAACA TCTGAAAGAA TGGATAGTTT AGATTCAGCA
    GCTTCTCGAG ACAGTATCTC CAGCTGCTAC ACTGAAGCAA CCATGCTGTT CTCAAGGCAG
    TCAACCCTTG ATGATTTAGA TGGACCAGAC ACTGTTCCTA AAACAAGTGA GCGCGCTCGA
    CCTAGGCTTC GTAAAATGCA GAGCATGGAT ATGTCCTCTT CATCAGCTGA CAGTGGCAGT
    ATAGTGTCAA GCGAGCCTAC ACAGGTCACC ATGCTCTATT CTCGTCAGGG GACGACAGAA
    ACCATTGAGG AGGTAGAAGG AGAGCATGAA GAGGAAGGAG CTCATTCTGC TTCAAGGCAG
    GATGAAGAAG AGGTGGAAGA TTATAGCCTG GGTTCAGAAG GAGCTGCATA TACTCCCGAC
    ACCGATGTAC CATTGTACTC CACTGTGGAC AGCAATGCAG AAGCACCACC TTCCTATAGC
    AAAGCTGTCA GCTTTGAACA CCTTCCTTTT GGCTCCCCAG ATGACTCAGC TGGAAAGAGT
    CTCATGATGG TGAGCCCAGA TGACAGCCGG ACGGACAGAC TTAATGATGC AATTCTCCCT
    CCATTGACGC ACGAGCTAAC GGCTAGTGAG CTGCTTCTGA ACAAGATGTT TCATGATGAT
    GAGCTAGAGG AATCAGAGAA ATTCTATGTT GGTCAGCCTC GAGTCTTGCT TCTTATTTAT
    GCGCTGTACA ATACTCTGGT GGCCCGGTCA GAAATGGTTT GCTATTTTGT CATCATCCTT
    AACCACATGA TCTCTGCCTC TATGATAACG CTGGTGCTGC CCATCCTTAT CTTTCTTTGG
    GCCATGCTGT CTGTCCCTCG ACCAAGCAAA CGTTTCTGGA TGACTGCCAT TGTCTACACT
    GAGGTAGCCA TTGTCATCAA GTACTTCTTC CAGTTTGGCT TCTTTCCTTG GAATAAATAT
    GTGGATTACA CAAAAGATAA ACCTTATCAT CCACCCAATA TTATTGGGAT AGAGAAGAAA
    GAGGGCTATG TGCACTATGA TCTTGTTCAG CTCCTTGCTT TATTTTTCCA CAGATCCATT
    TTAAAGTGCC ATGGATTGTG GGATGAAGAT GAGAAAGGGG ATAACTCCAG TAATAAAGGA
    GATACTGATG ATGAGCTTTC TCTGCCGGGA GGCAGGAGAG ACTCTTCTGG GTCTCTGAAG
    TCGGTCAATC TTGCAGCATC AGTGGAGTCC ATCCATGTGC ACTTCCCTGA GCAGCAGACA
    GCAATCAGGA GGAAGAGCTC CAGCAGTGCA TCACAGCTTT CTCACAGATC CAGCTTCTCC
    TCACACAGAT CTAAGAGAGG AAGCACCAGT ACAAGGAACA GCAGTCAGAA AGGGAGCAGT
    GTGCTAAGCA TCAAGCAAAA ATCTAGGAAA GAACTTCTTA TGGAAAAGTT TCGAGAGCAA
    ATGATCAAAG CCAAAGCCTT TACAATTAAA AAGACTCTCC AAGTGTATGT GCCCATCAGA
    CAATTTTTCT ACAATCTTAT TCATCCAGAC TACAGTGCAG TGACTGATGT ATATGTGCTG
    ATGTTCCTCG CAGATACAGT GGACTTCATC ATCATTGTCT TTGGATTTTG GGCTTTTGGG
    AAACACTCTG CAGCAGCAGA TATTACCTCT TCATTATCAG AGGACCAGGT TCCAGAGGCA
    TTTTTGGTGA TGGTGCTCAT TCAGTTTGGT ACCATGGTGG TAGACCGAGC GCTCTACCTC
    AAAAAGACAG TCATGGGAAA GGTCATCTTC CAGGTCATCC TTGTTTTTGG GATTCATTTC
    TGGATGTTCT TTATCTTGCC TGGAGTGACA GAAAGAAAAT TTAGCCAGAA CACCGTTGCC
    CAGCTCTGGT ATTTTGTGAA ATGTGTTTAT TTTGGCTTAT CAGCATATCA GATACGCTGC
    GGATATCCAA CTCGTGTTCT TGGCAACTTC CTCACAAAAA GTTATAACTA TGTGAACCTG
    TTCTTATTTC AAGGATTCCG CCTGGTTCCA TTTTTGACTG AGCTGAGAGC AGTAATGGAC
    TGGGTTTGGA CAGATACCAC TCTCAGTCTC TCCAGCTGGA TCTGTGTTGA AGATATCTAT
    GCTCATATAT TCATCCTGAA GTGCTGGCGA GAGTCAGAAA AGAGATATCC CCAACCAAGA
    GGCCAGAAGA AAAAGAAGGT TGTGAAGTAT GGAATGGGTG GCATGATTAT CGTCTTGCTG
    ATTTGTATTG TCTGGTTCCC ACTGCTCTTC ATGTCTTTAA TCAAATCTGT TGCAGGCATC
    ACCAACAAGC CTCTAGATGT ATCAATCACA ATAACTCTAG GAGGTTATCA GCCTATTTTT
    ACAATGAGTG CTCAACAGAG TCAGCTCAAG GATTTGAATC AGACTGGTTT CAGTGCATTT
    CTGGGAAGCT ATAGGGGTAA CACTGCTGCT TTGCAGTTTT TAGAGGGCTA TGGAAAAGAA
    GATATAACCC TAGCAGATCT AGAAGGGAAT TCAAACTCCT TATGGACCAT CAGCCCTCCC
    AGTAGAGAGA AAATGATACA GGGACTGCTG GATTTTTCAG CTGAGTTCAC TGTAGTACTT
    TCATGGAGCA TTCAAAGAAA TCTCACTCTT GGTGCCAAAG CAGAAATAGC ATCAGATAAA
    CTTACCTTTG GCCTACCAGA GAAAACCAGA AGAGATATTG CTACAATGAT GTCTGGAAAG
    CCATTGGAAA AGGTAACATT AGAAACAGTG TATCCATACT ACATCAAGGC TCCTAGTGAT
    TCTCTGGCAA AACCCATAAA GCAGCTATTG ACAGACTGCA GATGGGAAAA TATCACAGTT
    TCCCTGGTCA AAAATGTGTC TGAAGAGGGA GTTCGTGAGT GGTGGGTTTT GAATCAGCTT
    GGAAAAAGAT ACAAAACAAA TGAAGAGAGC CTTGAACTCT TCATATTCAG TGATAAAGTC
    AGTCCTCCAA GCCTTGGATT CTTGGCTGGA TATGGCATCA TGGGACTATA CGCGTCAGTC
    GTCCTGGTGA TAGGGAAGTT CGTCCGTGAG TTTTTCAGTG GGATTTCTCA CTCCATCATG
    TTTGAGGAGC TACCCAATGT AGACCGAATC TTGAAGTTGT GTACTGACAT TTTCTTAGTA
    CGAGAGACTG GAGAACTGGA ACTAGAAGAA GACCTGTACG CCAAACTAAT CTTCCTGTAT
    CGCTCACCAG AGACTATGAT CAAGTGGACT AGGGAAAAAA CTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146280
    Clone ID Related Accession (Same CDS sequence) XM_025147821.1
    Accession Version XM_025147821.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCACCG AGGTGCTGTG CGGGCTCACC TTCAGGCTGC TGCTGCCCGT GTGCCTGGCT 
    GCCGCCTGTG CTTTCCGTTA CAATGGACTC TCCTTTGTCT ACCTCATCTA CCTACTGCTC
    ATTCCTTTGT TTTCAGAACC CACAAAAACA ACGATGCAAG GGCATACAGG ACGATTATTA
    AAATCTCTGT GTTTCACCAG TCTATCATTT CTGCTGCTGC ACATCATCTT TCAGATTACA
    ATAAACAGTC TTGAAGCTGG AAGAACTATT GAACCTGGTT TTAACTGTTC CACATGGGAG
    AAGACATTAC GGCAGATTGG TTTTGAAAGT GTGAAAGGAG CAGATGCCGG CAACGTGATC
    AGACTATTTG TACCAGACAT CGGGATGTTC ATTGCGAGCC TGACAATATG GCTCCTCTGC
    CGCAACATGG TTCAGAAGGC AATAAATGAG GATGCAGTGC ATTGCAACGC GCAGTTTGAA
    AATGAGGAAA TGGCTGTAAG AGAAAAGTTA GAGCCAGACG ATGCTTTGAT GTATGAAGAA
    GACCTGGATG ATGACTGTGG TGAAGCAGAA TTCGAGGAAA CAATGAAATT AAAACTGCTG
    CGTAGAATTG CCTCCCTAGC ATCCAAACTG AGAGAGTTTA TTGGCAACAT GATAATCACC
    ACTGGAAAAG TTGTTGTTAC AATTTTACTT GGTTCAGCAG GTATGATGGT GCCATCCTTG
    ACCTCAGCTG TATATTTCTT TGTATTTTTG GGCCTGTGCA CGTGGTGGTC CTGTTGCCAA
    GCATTTGATC CGCTGATATT CAGCTGTCTG TGTGTATTAA TGGCTATATT CAGTGCAGGG
    CACTTGATTG GACTTTATCT GTACCAGTTG CAGTTCTTCC AGGAGGTCGT TCCACCAAAA
    GACTTCTATG CCAGGTTGTT TGGTGTCACT TCTCTTACGC AGACAAATTG CTCAAGCACT
    TGGATGATCA TCAAAAATCA AGAGTTACAC TGGTATCACC ATGCCAATCC AGTTCTACTG
    CTGGTGATGT ACTACACCCT TGCAACTCTG ATCCGCCTTT GGCTGCAAGA GCCCATTCAG
    GTACCTGAGG AAGAGAAATC TGAGAGCAGG GAAGATGACA AAGAGATACC CTGCAGCCCA
    ATTCCAATGA CAGCAGAGAG AAGACGCAGT CTGTGGTACG CAAGCCACTA TACAACTGAT
    GAGAGAAAAC TTCTGTCAAT GACCCAAGAT GAATACAAGC CATCGGATGT TCTGCTGGTA
    ACAGTGAACG GGAATCCTGC TGACTATCAC ACCATCCACC CCTCACTGCC ATTAGAGAAT
    GGTCCAGCCA AAGCAGACAT GTACTCAACA CCACAGTACA AATGGGAGCC CTCGGATAAC
    ATGTCAGAAA AGGAAGAGGA GGAAGTAGAG GAGGAAGAAG AAGTTCCTCA GGAGGAAAAA
    GAAAATGTTA AATTGCATGC CCTGGTCTCT GTGTTCCAAT TTATCATGAA GCAAAGTTAC
    ATTTGTGCTT TGATAGCTAT GATGGCATGG AGTATCACGT ATCACAGCTG GTTGACTTTT
    GTGCTCTTGA TCTGGTCTTG CACGCTGTGG ATGATTCGAG ACCGAAGGAA ATATGCTATG
    ATCAGTTCAC CGTTCATGGT TTTCTATGGA AATTTACTGC TAATATTACA ATATATATGG
    AGTATTGAGC TCAAGAATGA TGAGCTTCCT CAAGTATCAG GTTTTTTAGA AAGAAAGGAA
    CCTGGAGAGT TGGCATCAAA GATTCTTTTC ACGATTACTT TCTGGCTACT GCTTAGGCAA
    CACCTCACTG AACAGAAAGC ACTTCAGTTA AAAGAAGCTA CTTTATCTGA GGTCAAAGTT
    GGAAATGAAG AAGATGAAGA AAAAGAAGAG GGAGAGTTGC AAGAGGGAGA AGTGGCAGAA
    GAAGAGGAGG AGGAGGAGGA GGAACAGGAA GATGATGATG AGGATGAGCA GGATATTATG
    AAAGTTCTGG GGAAGCTGGT GATGGCTATG TTCATCAAGT ACTGGATTTA TGTCTGTGGA
    GGCATGTTCT TCTTCGTCAG CTTTGAGGGT AGAATTGTCA TGTACAAAAT CATCTACATG
    GTACTATTCC TGTTCTGTGT GGCCCTCTAT CAGGTACACT ATGAATGGTG GAGAAAGATT
    CTGAAATACT TTTGGATGTC AGTTGTTGTT TACACGATGT TGGTACTTAT CTTCATCTAC
    ACATACCAGT TTGAGTCATT CCCTGGGTTG TGGAAAAATA TGACAGGCCT GGATGAAAAC
    AAACTAGCAG ACCTTGGTTT AAAAAGATTT TCTGTGGCTG AGCTATTCAC TCGCATCTTT
    ATACCCACCT CCTTCCTACT GGCGTGTATC TTGCACCTTC ACTATTTCCA TGACCGTTTC
    CTCCAGCTCA CTGATTTAAA AGCTGTAACC AGCAAACAGG ACAACACTAT TTACAGACTG
    GTGCATCAAG ATGGAAGCCT GCCTGACATA ACTATGATGA ATTTAACAGC CAGCAGTGAA
    AAGGAAGAGG ATAAAATGCT GAAAGAGGCT GGTGAGAAAA GAATGGAAGA GCCAGATGGG
    GAGGGAGGGA AAGGGAGGGC TGAAAAAGGA AAAGAAGAAG AAGAGGAAGA TGAGGAGAAG
    GAAGATGATG ATGTAGACGA TGAGTCAGAG GAAGAAGAAA CTACAGACCT AAGAAACAAG
    TGGCATCTGG TGATTGACCG CCTTACAGTG CTGTTTTTGA AATTCCTGGA GTACTTTCAT
    AAGATGCAAG TCTTTGTCTG GTGGCTTTTG GAGCTGCATA TCATCAAAAT CGTCTCCTCT
    TACATCATCT GGGTCACAGT AAAAGAGGTG TCTCTGTTTA ACTTTGTGTT TTTAATTGCT
    TGGGCTCTTG CTTTGCCATA TGCTCAGTTT CGCCCATTGG CATCAAGTAT CTGCACTGTT
    TGGACATGTG TGATTATTGT CTGCAAAATG TTGTACCAGC TCACATCTAT TGATCCCAGC
    ACTTTTTCCA GTAACTGTAC ATTGCCCAGA GAAAATGAAA CAAAGGTTGA TTTAGAAGAG
    CTCAAAACAT CAGTCCTCTA CAGTGGTCCG GTTGATCCTG CAGAATGGGT TGGATTGAGG
    AAGTCTTATC CCCTACTTTT ATACTTAAGG AATAACTTAC TGATGCTGGC TATTCTGGCT
    TTTGAAGTTA CAATTTACCG TCATCAAGAG TACTACAGAT GTCGAAATAA CCTTACTGCA
    CCTGTAACTA AAACTATTTT CCATGATATT ACAAGAGCAC ATCTGGATGA TGGACTGGTA
    AACTGTGTCA AGTATTTCAT AAACTACTTC TTCTACAAGT TTGGTTTAGA GACCTGTTTT
    CTTTTATCAG TGAATGTAAT TGGTCAACGG ATGGATTTTT ATGCCATGAT TCATGCCTTC
    TGGTTGATTG CTGTATTGTA TCGACGTAGA AGAAAAGCTA TTGCAGAGAT CTGGCCAAAG
    TATTGCTGTT TTCTGGCATG CATCATTACA TTCCAGTATT TCCTTTGTAT TGGCATTCCA
    CCTGCTCCTT GTAAAGACTA TCCATGGAGA AGTGGTAATG CCAACTTCAA CTCAAATATC
    ATAAAGTGGT TATACTTTCC AGACTTCATT GTGAGACCAA ACCCTGTCTT CCTTGTCTAT
    GATTTCATGT TGCTGCTGTG TGCATCCTTA CAAAGGCAAA CGTTTGAGGA TGAAAATAAA
    GCTGCTGTGC GAATCATGGC TGGAGACAAC GTGGAAATTT GTATGAATCT TGAAGCAGCA
    TCTTTTAGCC AGCATAATCC TGTTCCAGAC TTCATTCACT GCCGGTCCTA CTTAGATATG
    TATAAGGTGA TAATCTTTAG TTACCTCTTC TGGTTTGTGC TTACTATAAT CTTCATAACG
    GGAACAACGA GGATCAGCAT ATTCTGTATG GGCTACTTGG TTGCCTGCTT TTATTTCCTT
    CTCTTTGGTG GAGATCTGCT GCTGAAACCC ATCAGGAGCA TCCTGCGCTA CTGGGACTGG
    CTGATTGCAT ACAATGTTTT TGTGATCACC ATGAAGAACA TCCTCTCGAT AGGAGCATGT
    GGCTACATTG AGTCTTTAAT TCAAAATAGT TGCTGGTTGA TTCAGGCATT TAGTCTGGCT
    TGCACAGTTA AAGGCTACCG TATCCCAACC AACAATGCAG ACTGTAAACT ACCTAGTGGA
    GAAGCAGGAA TCATCTGGGA CAGTATATGT TTTGCCTTTC TACTGCTGCA AAGAAGAGTC
    TTCATGAGTT ACTACTTCCT CCACGTGGTT GCAGATATAA AAGCTTCTCA GATCCTAGCA
    TCAAGGGGTG CTGAGCTTTT CCAGGCCACA ATTGTTAAGG CAGTAAAGGC AAGAATTGAA
    GAGGAAAAAA AATCAATGGA TCAACTGAAA AGACAAATGG ATCGCATCAA AGCCAGGCAG
    CAAAAATATA AAAAGGGTAA AGAGAGGATG CTAAGCATGA CACAGGATTC CACAGAGGGG
    CCTGAAATTC GGAAGGTTTC GGAAGAAGAT GATGAAGGAG AAGCAGACAA AGAGAAAGCC
    AAGGGCAAAA AAAAGCTGTG GTGGCGGCCT TGGGTTGATC ATGCTTCCAT GGTCAGAAGT
    GGAAATTATT ATTTGTTTGA GACGGACAGT GAAGAGGAAG AGGAAGAAGA GAAAAAAGAA
    GAGGAAGAGC CTCGAAAAAA ATCTGCATTT CAGTTTGTTT ATCAGGCCTG GATTACTGAT
    CCTAAAACTG CGCTACGACA GAGGCGCAAG GAAAAGAAAA GCTTTCAAAA AGAGGAGAAG
    CGAAGGCGTA AAGTATATGG GGATGCAGGA AGTACTGATG CAGACTGCGA AGACAGTGAA
    GAAGAACCTG TCAAGAAGAA GTCTGATGGC CCAGATAACA TCATCAAGAG GATATTTAAT
    ATTTTGAAGT TCACTTGGGT CCTTTTCCTG GCAACACTAG ACAGTTTTAC AGCTTGGCTT
    AATTCCATCT CAAGAGAACA CATCGATATC TCTACTGTGC TGAGAATCGA ACGTTGTATG
    CTGACCAGGG AGATTAAGAA GGGAAACGTT CCTACCCGGG AGAGCATCCA TATGTATTAC
    CAGAACCATA TGATGAAACT TTCTAAGGAG TCAGGACTGG ACTCTATTGA TAAAAATCCT
    GGGCAAGCTT CTGGTTTGCA AACATCTGAA AGAATGGATA GTTTAGATTC AGCAGCTTCT
    CGAGACAGTA TCTCCAGCTG CTACACTGAA GCAACCATGC TGTTCTCAAG GCAGTCAACC
    CTTGATGATT TAGATGGACC AGACACTGTT CCTAAAACAA GTGAGCGCGC TCGACCTAGG
    CTTCGTAAAA TGCAGAGCAT GGATATGTCC TCTTCATCAG CTGACAGTGG CAGTATAGTG
    TCAAGCGAGC CTACACAGGT CACCATGCTC TATTCTCGTC AGGGGACGAC AGAAACCATT
    GAGGAGGTAG AAGGAGAGCA TGAAGAGGAA GGAGCTCATT CTGCTTCAAG GCAGGATGAA
    GAAGAGGTGG AAGATTATAG CCTGGGTTCA GAAGGAGCTG CATATACTCC CGACACCGAT
    GTACCATTGT ACTCCACTGT GGACAGCAAT GCAGAAGCAC CACCTTCCTA TAGCAAAGCT
    GTCAGCTTTG AACACCTTCC TTTTGGCTCC CCAGATGACT CAGCTGGAAA GAGTCTCATG
    ATGGTGAGCC CAGATGACAG CCGGACGGAC AGACTTAATG ATGCAATTCT CCCTCCATTG
    ACGCACGAGC TAACGGCTAG TGAGCTGCTT CTGAACAAGA TGTTTCATGA TGATGAGCTA
    GAGGAATCAG AGAAATTCTA TGTTGGTCAG CCTCGAGTCT TGCTTCTTAT TTATGCGCTG
    TACAATACTC TGGTGGCCCG GTCAGAAATG GTTTGCTATT TTGTCATCAT CCTTAACCAC
    ATGATCTCTG CCTCTATGAT AACGCTGGTG CTGCCCATCC TTATCTTTCT TTGGGCCATG
    CTGTCTGTCC CTCGACCAAG CAAACGTTTC TGGATGACTG CCATTGTCTA CACTGAGGTA
    GCCATTGTCA TCAAGTACTT CTTCCAGTTT GGCTTCTTTC CTTGGAATAA ATATGTGGAT
    TACACAAAAG ATAAACCTTA TCATCCACCC AATATTATTG GGATAGAGAA GAAAGAGGGC
    TATGTGCACT ATGATCTTGT TCAGCTCCTT GCTTTATTTT TCCACAGATC CATTTTAAAG
    TGCCATGGAT TGTGGGATGA AGATGAGAAA GGGGATAACT CCAGTAATAA AGGAGATACT
    GATGATGAGC TTTCTCTGCC GGGAGGCAGG AGAGACTCTT CTGGGTCTCT GAAGTCGGTC
    AATCTTGCAG CATCAGTGGA GTCCATCCAT GTGCACTTCC CTGAGCAGCA GACAGCAATC
    AGGAGGAAGA GCTCCAGCAG TGCATCACAG CTTTCTCACA GATCCAGCTT CTCCTCACAC
    AGATCTAAGA GAGGAAGCAC CAGTACAAGG AACAGCAGTC AGAAAGGGAG CAGTGTGCTA
    AGCATCAAGC AAAAATCTAG GAAAGAACTT CTTATGGAAA AGTTTCGAGA GCAAATGATC
    AAAGCCAAAG CCTTTACAAT TAAAAAGACT CTCCAAGTGT ATGTGCCCAT CAGACAATTT
    TTCTACAATC TTATTCATCC AGACTACAGT GCAGTGACTG ATGTATATGT GCTGATGTTC
    CTCGCAGATA CAGTGGACTT CATCATCATT GTCTTTGGAT TTTGGGCTTT TGGGAAACAC
    TCTGCAGCAG CAGATATTAC CTCTTCATTA TCAGAGGACC AGGTTCCAGA GGCATTTTTG
    GTGATGGTGC TCATTCAGTT TGGTACCATG GTGGTAGACC GAGCGCTCTA CCTCAAAAAG
    ACAGTCATGG GAAAGGTCAT CTTCCAGGTC ATCCTTGTTT TTGGGATTCA TTTCTGGATG
    TTCTTTATCT TGCCTGGAGT GACAGAAAGA AAATTTAGCC AGAACACCGT TGCCCAGCTC
    TGGTATTTTG TGAAATGTGT TTATTTTGGC TTATCAGCAT ATCAGATACG CTGCGGATAT
    CCAACTCGTG TTCTTGGCAA CTTCCTCACA AAAAGTTATA ACTATGTGAA CCTGTTCTTA
    TTTCAAGGAT TCCGCCTGGT TCCATTTTTG ACTGAGCTGA GAGCAGTAAT GGACTGGGTT
    TGGACAGATA CCACTCTCAG TCTCTCCAGC TGGATCTGTG TTGAAGATAT CTATGCTCAT
    ATATTCATCC TGAAGTGCTG GCGAGAGTCA GAAAAGAGAT ATCCCCAACC AAGAGGCCAG
    AAGAAAAAGA AGGTTGTGAA GTATGGAATG GGTGGCATGA TTATCGTCTT GCTGATTTGT
    ATTGTCTGGT TCCCACTGCT CTTCATGTCT TTAATCAAAT CTGTTGCAGG CATCACCAAC
    AAGCCTCTAG ATGTATCAAT CACAATAACT CTAGGAGGTT ATCAGCCTAT TTTTACAATG
    AGTGCTCAAC AGAGTCAGCT CAAGGATTTG AATCAGACTG GTTTCAGTGC ATTTCTGGGA
    AGCTATAGGG GTAACACTGC TGCTTTGCAG TTTTTAGAGG GCTATGGAAA AGAAGATATA
    ACCCTAGCAG ATCTAGAAGG GAATTCAAAC TCCTTATGGA CCATCAGCCC TCCCAGTAGA
    GAGAAAATGA TACAGGGACT GCTGGATTTT TCAGCTGAGT TCACTGTAGT ACTTTCATGG
    AGCATTCAAA GAAATCTCAC TCTTGGTGCC AAAGCAGAAA TAGCATCAGA TAAACTTACC
    TTTGGCCTAC CAGAGAAAAC CAGAAGAGAT ATTGCTACAA TGATGTCTGG AAAGCCATTG
    GAAAAGGTAA CATTAGAAAC AGTGTATCCA TACTACATCA AGGCTCCTAG TGATTCTCTG
    GCAAAACCCA TAAAGCAGCT ATTGACAGAC TGCAGATGGG AAAATATCAC AGTTTCCCTG
    GTCAAAAATG TGTCTGAAGA GGGAGTTCGT GAGTGGTGGG TTTTGAATCA GCTTGGAAAA
    AGATACAAAA CAAATGAAGA GAGCCTTGAA CTCTTCATAT TCAGTGATAA AGTCAGTCCT
    CCAAGCCTTG GATTCTTGGC TGGATATGGC ATCATGGGAC TATACGCGTC AGTCGTCCTG
    GTGATAGGGA AGTTCGTCCG TGAGTTTTTC AGTGGGATTT CTCACTCCAT CATGTTTGAG
    GAGCTACCCA ATGTAGACCG AATCTTGAAG TTGTGTACTG ACATTTTCTT AGTACGAGAG
    ACTGGAGAAC TGGAACTAGA AGAAGACCTG TACGCCAAAC TAATCTTCCT GTATCGCTCA
    CCAGAGACTA TGATCAAGTG GACTAGGGAA AAAACTAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003589.1
    CDS220..8601
    Translation

    Target ORF information:

    RefSeq Version XM_025147821.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147821.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCACCG AGGTGCTGTG CGGGCTCACC TTCAGGCTGC TGCTGCCCGT GTGCCTGGCT 
    GCCGCCTGTG CTTTCCGTTA CAATGGACTC TCCTTTGTCT ACCTCATCTA CCTACTGCTC
    ATTCCTTTGT TTTCAGAACC CACAAAAACA ACGATGCAAG GGCATACAGG ACGATTATTA
    AAATCTCTGT GTTTCACCAG TCTATCATTT CTGCTGCTGC ACATCATCTT TCAGATTACA
    ATAAACAGTC TTGAAGCTGG AAGAACTATT GAACCTGGTT TTAACTGTTC CACATGGGAG
    AAGACATTAC GGCAGATTGG TTTTGAAAGT GTGAAAGGAG CAGATGCCGG CAACGTGATC
    AGACTATTTG TACCAGACAT CGGGATGTTC ATTGCGAGCC TGACAATATG GCTCCTCTGC
    CGCAACATGG TTCAGAAGGC AATAAATGAG GATGCAGTGC ATTGCAACGC GCAGTTTGAA
    AATGAGGAAA TGGCTGTAAG AGAAAAGTTA GAGCCAGACG ATGCTTTGAT GTATGAAGAA
    GACCTGGATG ATGACTGTGG TGAAGCAGAA TTCGAGGAAA CAATGAAATT AAAACTGCTG
    CGTAGAATTG CCTCCCTAGC ATCCAAACTG AGAGAGTTTA TTGGCAACAT GATAATCACC
    ACTGGAAAAG TTGTTGTTAC AATTTTACTT GGTTCAGCAG GTATGATGGT GCCATCCTTG
    ACCTCAGCTG TATATTTCTT TGTATTTTTG GGCCTGTGCA CGTGGTGGTC CTGTTGCCAA
    GCATTTGATC CGCTGATATT CAGCTGTCTG TGTGTATTAA TGGCTATATT CAGTGCAGGG
    CACTTGATTG GACTTTATCT GTACCAGTTG CAGTTCTTCC AGGAGGTCGT TCCACCAAAA
    GACTTCTATG CCAGGTTGTT TGGTGTCACT TCTCTTACGC AGACAAATTG CTCAAGCACT
    TGGATGATCA TCAAAAATCA AGAGTTACAC TGGTATCACC ATGCCAATCC AGTTCTACTG
    CTGGTGATGT ACTACACCCT TGCAACTCTG ATCCGCCTTT GGCTGCAAGA GCCCATTCAG
    GTACCTGAGG AAGAGAAATC TGAGAGCAGG GAAGATGACA AAGAGATACC CTGCAGCCCA
    ATTCCAATGA CAGCAGAGAG AAGACGCAGT CTGTGGTACG CAAGCCACTA TACAACTGAT
    GAGAGAAAAC TTCTGTCAAT GACCCAAGAT GAATACAAGC CATCGGATGT TCTGCTGGTA
    ACAGTGAACG GGAATCCTGC TGACTATCAC ACCATCCACC CCTCACTGCC ATTAGAGAAT
    GGTCCAGCCA AAGCAGACAT GTACTCAACA CCACAGTACA AATGGGAGCC CTCGGATAAC
    ATGTCAGAAA AGGAAGAGGA GGAAGTAGAG GAGGAAGAAG AAGTTCCTCA GGAGGAAAAA
    GAAAATGTTA AATTGCATGC CCTGGTCTCT GTGTTCCAAT TTATCATGAA GCAAAGTTAC
    ATTTGTGCTT TGATAGCTAT GATGGCATGG AGTATCACGT ATCACAGCTG GTTGACTTTT
    GTGCTCTTGA TCTGGTCTTG CACGCTGTGG ATGATTCGAG ACCGAAGGAA ATATGCTATG
    ATCAGTTCAC CGTTCATGGT TTTCTATGGA AATTTACTGC TAATATTACA ATATATATGG
    AGTATTGAGC TCAAGAATGA TGAGCTTCCT CAAGTATCAG GTTTTTTAGA AAGAAAGGAA
    CCTGGAGAGT TGGCATCAAA GATTCTTTTC ACGATTACTT TCTGGCTACT GCTTAGGCAA
    CACCTCACTG AACAGAAAGC ACTTCAGTTA AAAGAAGCTA CTTTATCTGA GGTCAAAGTT
    GGAAATGAAG AAGATGAAGA AAAAGAAGAG GGAGAGTTGC AAGAGGGAGA AGTGGCAGAA
    GAAGAGGAGG AGGAGGAGGA GGAACAGGAA GATGATGATG AGGATGAGCA GGATATTATG
    AAAGTTCTGG GGAAGCTGGT GATGGCTATG TTCATCAAGT ACTGGATTTA TGTCTGTGGA
    GGCATGTTCT TCTTCGTCAG CTTTGAGGGT AGAATTGTCA TGTACAAAAT CATCTACATG
    GTACTATTCC TGTTCTGTGT GGCCCTCTAT CAGGTACACT ATGAATGGTG GAGAAAGATT
    CTGAAATACT TTTGGATGTC AGTTGTTGTT TACACGATGT TGGTACTTAT CTTCATCTAC
    ACATACCAGT TTGAGTCATT CCCTGGGTTG TGGAAAAATA TGACAGGCCT GGATGAAAAC
    AAACTAGCAG ACCTTGGTTT AAAAAGATTT TCTGTGGCTG AGCTATTCAC TCGCATCTTT
    ATACCCACCT CCTTCCTACT GGCGTGTATC TTGCACCTTC ACTATTTCCA TGACCGTTTC
    CTCCAGCTCA CTGATTTAAA AGCTGTAACC AGCAAACAGG ACAACACTAT TTACAGACTG
    GTGCATCAAG ATGGAAGCCT GCCTGACATA ACTATGATGA ATTTAACAGC CAGCAGTGAA
    AAGGAAGAGG ATAAAATGCT GAAAGAGGCT GGTGAGAAAA GAATGGAAGA GCCAGATGGG
    GAGGGAGGGA AAGGGAGGGC TGAAAAAGGA AAAGAAGAAG AAGAGGAAGA TGAGGAGAAG
    GAAGATGATG ATGTAGACGA TGAGTCAGAG GAAGAAGAAA CTACAGACCT AAGAAACAAG
    TGGCATCTGG TGATTGACCG CCTTACAGTG CTGTTTTTGA AATTCCTGGA GTACTTTCAT
    AAGATGCAAG TCTTTGTCTG GTGGCTTTTG GAGCTGCATA TCATCAAAAT CGTCTCCTCT
    TACATCATCT GGGTCACAGT AAAAGAGGTG TCTCTGTTTA ACTTTGTGTT TTTAATTGCT
    TGGGCTCTTG CTTTGCCATA TGCTCAGTTT CGCCCATTGG CATCAAGTAT CTGCACTGTT
    TGGACATGTG TGATTATTGT CTGCAAAATG TTGTACCAGC TCACATCTAT TGATCCCAGC
    ACTTTTTCCA GTAACTGTAC ATTGCCCAGA GAAAATGAAA CAAAGGTTGA TTTAGAAGAG
    CTCAAAACAT CAGTCCTCTA CAGTGGTCCG GTTGATCCTG CAGAATGGGT TGGATTGAGG
    AAGTCTTATC CCCTACTTTT ATACTTAAGG AATAACTTAC TGATGCTGGC TATTCTGGCT
    TTTGAAGTTA CAATTTACCG TCATCAAGAG TACTACAGAT GTCGAAATAA CCTTACTGCA
    CCTGTAACTA AAACTATTTT CCATGATATT ACAAGAGCAC ATCTGGATGA TGGACTGGTA
    AACTGTGTCA AGTATTTCAT AAACTACTTC TTCTACAAGT TTGGTTTAGA GACCTGTTTT
    CTTTTATCAG TGAATGTAAT TGGTCAACGG ATGGATTTTT ATGCCATGAT TCATGCCTTC
    TGGTTGATTG CTGTATTGTA TCGACGTAGA AGAAAAGCTA TTGCAGAGAT CTGGCCAAAG
    TATTGCTGTT TTCTGGCATG CATCATTACA TTCCAGTATT TCCTTTGTAT TGGCATTCCA
    CCTGCTCCTT GTAAAGACTA TCCATGGAGA AGTGGTAATG CCAACTTCAA CTCAAATATC
    ATAAAGTGGT TATACTTTCC AGACTTCATT GTGAGACCAA ACCCTGTCTT CCTTGTCTAT
    GATTTCATGT TGCTGCTGTG TGCATCCTTA CAAAGGCAAA CGTTTGAGGA TGAAAATAAA
    GCTGCTGTGC GAATCATGGC TGGAGACAAC GTGGAAATTT GTATGAATCT TGAAGCAGCA
    TCTTTTAGCC AGCATAATCC TGTTCCAGAC TTCATTCACT GCCGGTCCTA CTTAGATATG
    TATAAGGTGA TAATCTTTAG TTACCTCTTC TGGTTTGTGC TTACTATAAT CTTCATAACG
    GGAACAACGA GGATCAGCAT ATTCTGTATG GGCTACTTGG TTGCCTGCTT TTATTTCCTT
    CTCTTTGGTG GAGATCTGCT GCTGAAACCC ATCAGGAGCA TCCTGCGCTA CTGGGACTGG
    CTGATTGCAT ACAATGTTTT TGTGATCACC ATGAAGAACA TCCTCTCGAT AGGAGCATGT
    GGCTACATTG AGTCTTTAAT TCAAAATAGT TGCTGGTTGA TTCAGGCATT TAGTCTGGCT
    TGCACAGTTA AAGGCTACCG TATCCCAACC AACAATGCAG ACTGTAAACT ACCTAGTGGA
    GAAGCAGGAA TCATCTGGGA CAGTATATGT TTTGCCTTTC TACTGCTGCA AAGAAGAGTC
    TTCATGAGTT ACTACTTCCT CCACGTGGTT GCAGATATAA AAGCTTCTCA GATCCTAGCA
    TCAAGGGGTG CTGAGCTTTT CCAGGCCACA ATTGTTAAGG CAGTAAAGGC AAGAATTGAA
    GAGGAAAAAA AATCAATGGA TCAACTGAAA AGACAAATGG ATCGCATCAA AGCCAGGCAG
    CAAAAATATA AAAAGGGTAA AGAGAGGATG CTAAGCATGA CACAGGATTC CACAGAGGGG
    CCTGAAATTC GGAAGGTTTC GGAAGAAGAT GATGAAGGAG AAGCAGACAA AGAGAAAGCC
    AAGGGCAAAA AAAAGCTGTG GTGGCGGCCT TGGGTTGATC ATGCTTCCAT GGTCAGAAGT
    GGAAATTATT ATTTGTTTGA GACGGACAGT GAAGAGGAAG AGGAAGAAGA GAAAAAAGAA
    GAGGAAGAGC CTCGAAAAAA ATCTGCATTT CAGTTTGTTT ATCAGGCCTG GATTACTGAT
    CCTAAAACTG CGCTACGACA GAGGCGCAAG GAAAAGAAAA GCTTTCAAAA AGAGGAGAAG
    CGAAGGCGTA AAGTATATGG GGATGCAGGA AGTACTGATG CAGACTGCGA AGACAGTGAA
    GAAGAACCTG TCAAGAAGAA GTCTGATGGC CCAGATAACA TCATCAAGAG GATATTTAAT
    ATTTTGAAGT TCACTTGGGT CCTTTTCCTG GCAACACTAG ACAGTTTTAC AGCTTGGCTT
    AATTCCATCT CAAGAGAACA CATCGATATC TCTACTGTGC TGAGAATCGA ACGTTGTATG
    CTGACCAGGG AGATTAAGAA GGGAAACGTT CCTACCCGGG AGAGCATCCA TATGTATTAC
    CAGAACCATA TGATGAAACT TTCTAAGGAG TCAGGACTGG ACTCTATTGA TAAAAATCCT
    GGGCAAGCTT CTGGTTTGCA AACATCTGAA AGAATGGATA GTTTAGATTC AGCAGCTTCT
    CGAGACAGTA TCTCCAGCTG CTACACTGAA GCAACCATGC TGTTCTCAAG GCAGTCAACC
    CTTGATGATT TAGATGGACC AGACACTGTT CCTAAAACAA GTGAGCGCGC TCGACCTAGG
    CTTCGTAAAA TGCAGAGCAT GGATATGTCC TCTTCATCAG CTGACAGTGG CAGTATAGTG
    TCAAGCGAGC CTACACAGGT CACCATGCTC TATTCTCGTC AGGGGACGAC AGAAACCATT
    GAGGAGGTAG AAGGAGAGCA TGAAGAGGAA GGAGCTCATT CTGCTTCAAG GCAGGATGAA
    GAAGAGGTGG AAGATTATAG CCTGGGTTCA GAAGGAGCTG CATATACTCC CGACACCGAT
    GTACCATTGT ACTCCACTGT GGACAGCAAT GCAGAAGCAC CACCTTCCTA TAGCAAAGCT
    GTCAGCTTTG AACACCTTCC TTTTGGCTCC CCAGATGACT CAGCTGGAAA GAGTCTCATG
    ATGGTGAGCC CAGATGACAG CCGGACGGAC AGACTTAATG ATGCAATTCT CCCTCCATTG
    ACGCACGAGC TAACGGCTAG TGAGCTGCTT CTGAACAAGA TGTTTCATGA TGATGAGCTA
    GAGGAATCAG AGAAATTCTA TGTTGGTCAG CCTCGAGTCT TGCTTCTTAT TTATGCGCTG
    TACAATACTC TGGTGGCCCG GTCAGAAATG GTTTGCTATT TTGTCATCAT CCTTAACCAC
    ATGATCTCTG CCTCTATGAT AACGCTGGTG CTGCCCATCC TTATCTTTCT TTGGGCCATG
    CTGTCTGTCC CTCGACCAAG CAAACGTTTC TGGATGACTG CCATTGTCTA CACTGAGGTA
    GCCATTGTCA TCAAGTACTT CTTCCAGTTT GGCTTCTTTC CTTGGAATAA ATATGTGGAT
    TACACAAAAG ATAAACCTTA TCATCCACCC AATATTATTG GGATAGAGAA GAAAGAGGGC
    TATGTGCACT ATGATCTTGT TCAGCTCCTT GCTTTATTTT TCCACAGATC CATTTTAAAG
    TGCCATGGAT TGTGGGATGA AGATGAGAAA GGGGATAACT CCAGTAATAA AGGAGATACT
    GATGATGAGC TTTCTCTGCC GGGAGGCAGG AGAGACTCTT CTGGGTCTCT GAAGTCGGTC
    AATCTTGCAG CATCAGTGGA GTCCATCCAT GTGCACTTCC CTGAGCAGCA GACAGCAATC
    AGGAGGAAGA GCTCCAGCAG TGCATCACAG CTTTCTCACA GATCCAGCTT CTCCTCACAC
    AGATCTAAGA GAGGAAGCAC CAGTACAAGG AACAGCAGTC AGAAAGGGAG CAGTGTGCTA
    AGCATCAAGC AAAAATCTAG GAAAGAACTT CTTATGGAAA AGTTTCGAGA GCAAATGATC
    AAAGCCAAAG CCTTTACAAT TAAAAAGACT CTCCAAGTGT ATGTGCCCAT CAGACAATTT
    TTCTACAATC TTATTCATCC AGACTACAGT GCAGTGACTG ATGTATATGT GCTGATGTTC
    CTCGCAGATA CAGTGGACTT CATCATCATT GTCTTTGGAT TTTGGGCTTT TGGGAAACAC
    TCTGCAGCAG CAGATATTAC CTCTTCATTA TCAGAGGACC AGGTTCCAGA GGCATTTTTG
    GTGATGGTGC TCATTCAGTT TGGTACCATG GTGGTAGACC GAGCGCTCTA CCTCAAAAAG
    ACAGTCATGG GAAAGGTCAT CTTCCAGGTC ATCCTTGTTT TTGGGATTCA TTTCTGGATG
    TTCTTTATCT TGCCTGGAGT GACAGAAAGA AAATTTAGCC AGAACACCGT TGCCCAGCTC
    TGGTATTTTG TGAAATGTGT TTATTTTGGC TTATCAGCAT ATCAGATACG CTGCGGATAT
    CCAACTCGTG TTCTTGGCAA CTTCCTCACA AAAAGTTATA ACTATGTGAA CCTGTTCTTA
    TTTCAAGGAT TCCGCCTGGT TCCATTTTTG ACTGAGCTGA GAGCAGTAAT GGACTGGGTT
    TGGACAGATA CCACTCTCAG TCTCTCCAGC TGGATCTGTG TTGAAGATAT CTATGCTCAT
    ATATTCATCC TGAAGTGCTG GCGAGAGTCA GAAAAGAGAT ATCCCCAACC AAGAGGCCAG
    AAGAAAAAGA AGGTTGTGAA GTATGGAATG GGTGGCATGA TTATCGTCTT GCTGATTTGT
    ATTGTCTGGT TCCCACTGCT CTTCATGTCT TTAATCAAAT CTGTTGCAGG CATCACCAAC
    AAGCCTCTAG ATGTATCAAT CACAATAACT CTAGGAGGTT ATCAGCCTAT TTTTACAATG
    AGTGCTCAAC AGAGTCAGCT CAAGGATTTG AATCAGACTG GTTTCAGTGC ATTTCTGGGA
    AGCTATAGGG GTAACACTGC TGCTTTGCAG TTTTTAGAGG GCTATGGAAA AGAAGATATA
    ACCCTAGCAG ATCTAGAAGG GAATTCAAAC TCCTTATGGA CCATCAGCCC TCCCAGTAGA
    GAGAAAATGA TACAGGGACT GCTGGATTTT TCAGCTGAGT TCACTGTAGT ACTTTCATGG
    AGCATTCAAA GAAATCTCAC TCTTGGTGCC AAAGCAGAAA TAGCATCAGA TAAACTTACC
    TTTGGCCTAC CAGAGAAAAC CAGAAGAGAT ATTGCTACAA TGATGTCTGG AAAGCCATTG
    GAAAAGGTAA CATTAGAAAC AGTGTATCCA TACTACATCA AGGCTCCTAG TGATTCTCTG
    GCAAAACCCA TAAAGCAGCT ATTGACAGAC TGCAGATGGG AAAATATCAC AGTTTCCCTG
    GTCAAAAATG TGTCTGAAGA GGGAGTTCGT GAGTGGTGGG TTTTGAATCA GCTTGGAAAA
    AGATACAAAA CAAATGAAGA GAGCCTTGAA CTCTTCATAT TCAGTGATAA AGTCAGTCCT
    CCAAGCCTTG GATTCTTGGC TGGATATGGC ATCATGGGAC TATACGCGTC AGTCGTCCTG
    GTGATAGGGA AGTTCGTCCG TGAGTTTTTC AGTGGGATTT CTCACTCCAT CATGTTTGAG
    GAGCTACCCA ATGTAGACCG AATCTTGAAG TTGTGTACTG ACATTTTCTT AGTACGAGAG
    ACTGGAGAAC TGGAACTAGA AGAAGACCTG TACGCCAAAC TAATCTTCCT GTATCGCTCA
    CCAGAGACTA TGATCAAGTG GACTAGGGAA AAAACTAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146281
    Clone ID Related Accession (Same CDS sequence) XM_025147822.1
    Accession Version XM_025147822.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAAGT TCTGCTGGTA ACAGTGAACG GGAATCCTGC TGACTATCAC
    ACCATCCACC CCTCACTGCC ATTAGAGAAT GGTCCAGCCA AAGCAGACAT GTACTCAACA
    CCACAGTACA AATGGGAGCC CTCGGATAAC ATGTCAGAAA AGGAAGAGGA GGAAGTAGAG
    GAGGAAGAAG AAGTTCCTCA GGAGGAAAAA GAAAATGTTA AATTGCATGC CCTGGTCTCT
    GTGTTCCAAT TTATCATGAA GCAAAGTTAC ATTTGTGCTT TGATAGCTAT GATGGCATGG
    AGTATCACGT ATCACAGCTG GTTGACTTTT GTGCTCTTGA TCTGGTCTTG CACGCTGTGG
    ATGATTCGAG ACCGAAGGAA ATATGCTATG ATCAGTTCAC CGTTCATGGT TTTCTATGGA
    AATTTACTGC TAATATTACA ATATATATGG AGTATTGAGC TCAAGAATGA TGAGCTTCCT
    CAAGTATCAG GTTTTTTAGA AAGAAAGGAA CCTGGAGAGT TGGCATCAAA GATTCTTTTC
    ACGATTACTT TCTGGCTACT GCTTAGGCAA CACCTCACTG AACAGAAAGC ACTTCAGTTA
    AAAGAAGCTA CTTTATCTGA GGTCAAAGTT GGAAATGAAG AAGATGAAGA AAAAGAAGAG
    GGAGAGTTGC AAGAGGGAGA AGTGGCAGAA GAAGAGGAGG AGGAGGAGGA GGAACAGGAA
    GATGATGATG AGGATGAGCA GGATATTATG AAAGTTCTGG GGAAGCTGGT GATGGCTATG
    TTCATCAAGT ACTGGATTTA TGTCTGTGGA GGCATGTTCT TCTTCGTCAG CTTTGAGGGT
    AGAATTGTCA TGTACAAAAT CATCTACATG GTACTATTCC TGTTCTGTGT GGCCCTCTAT
    CAGGTACACT ATGAATGGTG GAGAAAGATT CTGAAATACT TTTGGATGTC AGTTGTTGTT
    TACACGATGT TGGTACTTAT CTTCATCTAC ACATACCAGT TTGAGTCATT CCCTGGGTTG
    TGGAAAAATA TGACAGGCCT GGATGAAAAC AAACTAGCAG ACCTTGGTTT AAAAAGATTT
    TCTGTGGCTG AGCTATTCAC TCGCATCTTT ATACCCACCT CCTTCCTACT GGCGTGTATC
    TTGCACCTTC ACTATTTCCA TGACCGTTTC CTCCAGCTCA CTGATTTAAA AGCTGTAACC
    AGCAAACAGG ACAACACTAT TTACAGACTG GTGCATCAAG ATGGAAGCCT GCCTGACATA
    ACTATGATGA ATTTAACAGC CAGCAGTGAA AAGGAAGAGG ATAAAATGCT GAAAGAGGCT
    GGTGAGAAAA GAATGGAAGA GCCAGATGGG GAGGGAGGGA AAGGGAGGGC TGAAAAAGGA
    AAAGAAGAAG AAGAGGAAGA TGAGGAGAAG GAAGATGATG ATGTAGACGA TGAGTCAGAG
    GAAGAAGAAA CTACAGACCT AAGAAACAAG TGGCATCTGG TGATTGACCG CCTTACAGTG
    CTGTTTTTGA AATTCCTGGA GTACTTTCAT AAGATGCAAG TCTTTGTCTG GTGGCTTTTG
    GAGCTGCATA TCATCAAAAT CGTCTCCTCT TACATCATCT GGGTCACAGT AAAAGAGGTG
    TCTCTGTTTA ACTTTGTGTT TTTAATTGCT TGGGCTCTTG CTTTGCCATA TGCTCAGTTT
    CGCCCATTGG CATCAAGTAT CTGCACTGTT TGGACATGTG TGATTATTGT CTGCAAAATG
    TTGTACCAGC TCACATCTAT TGATCCCAGC ACTTTTTCCA GTAACTGTAC ATTGCCCAGA
    GAAAATGAAA CAAAGGTTGA TTTAGAAGAG CTCAAAACAT CAGTCCTCTA CAGTGGTCCG
    GTTGATCCTG CAGAATGGGT TGGATTGAGG AAGTCTTATC CCCTACTTTT ATACTTAAGG
    AATAACTTAC TGATGCTGGC TATTCTGGCT TTTGAAGTTA CAATTTACCG TCATCAAGAG
    TACTACAGAT GTCGAAATAA CCTTACTGCA CCTGTAACTA AAACTATTTT CCATGATATT
    ACAAGAGCAC ATCTGGATGA TGGACTGGTA AACTGTGTCA AGTATTTCAT AAACTACTTC
    TTCTACAAGT TTGGTTTAGA GACCTGTTTT CTTTTATCAG TGAATGTAAT TGGTCAACGG
    ATGGATTTTT ATGCCATGAT TCATGCCTTC TGGTTGATTG CTGTATTGTA TCGACGTAGA
    AGAAAAGCTA TTGCAGAGAT CTGGCCAAAG TATTGCTGTT TTCTGGCATG CATCATTACA
    TTCCAGTATT TCCTTTGTAT TGGCATTCCA CCTGCTCCTT GTAAAGACTA TCCATGGAGA
    AGTGGTAATG CCAACTTCAA CTCAAATATC ATAAAGTGGT TATACTTTCC AGACTTCATT
    GTGAGACCAA ACCCTGTCTT CCTTGTCTAT GATTTCATGT TGCTGCTGTG TGCATCCTTA
    CAAAGGCAAA CGTTTGAGGA TGAAAATAAA GCTGCTGTGC GAATCATGGC TGGAGACAAC
    GTGGAAATTT GTATGAATCT TGAAGCAGCA TCTTTTAGCC AGCATAATCC TGTTCCAGAC
    TTCATTCACT GCCGGTCCTA CTTAGATATG TATAAGGTGA TAATCTTTAG TTACCTCTTC
    TGGTTTGTGC TTACTATAAT CTTCATAACG GGAACAACGA GGATCAGCAT ATTCTGTATG
    GGCTACTTGG TTGCCTGCTT TTATTTCCTT CTCTTTGGTG GAGATCTGCT GCTGAAACCC
    ATCAGGAGCA TCCTGCGCTA CTGGGACTGG CTGATTGCAT ACAATGTTTT TGTGATCACC
    ATGAAGAACA TCCTCTCGAT AGGAGCATGT GGCTACATTG AGTCTTTAAT TCAAAATAGT
    TGCTGGTTGA TTCAGGCATT TAGTCTGGCT TGCACAGTTA AAGGCTACCG TATCCCAACC
    AACAATGCAG ACTGTAAACT ACCTAGTGGA GAAGCAGGAA TCATCTGGGA CAGTATATGT
    TTTGCCTTTC TACTGCTGCA AAGAAGAGTC TTCATGAGTT ACTACTTCCT CCACGTGGTT
    GCAGATATAA AAGCTTCTCA GATCCTAGCA TCAAGGGGTG CTGAGCTTTT CCAGGCCACA
    ATTGTTAAGG CAGTAAAGGC AAGAATTGAA GAGGAAAAAA AATCAATGGA TCAACTGAAA
    AGACAAATGG ATCGCATCAA AGCCAGGCAG CAAAAATATA AAAAGGGTAA AGAGAGGATG
    CTAAGCATGA CACAGGATTC CACAGAGGGG CCTGAAATTC GGAAGGTTTC GGAAGAAGAT
    GATGAAGGAG AAGCAGACAA AGAGAAAGCC AAGGGCAAAA AAAAGCTGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGAAGT GGAAATTATT ATTTGTTTGA GACGGACAGT
    GAAGAGGAAG AGGAAGAAGA GAAAAAAGAA GAGGAAGAGC CTCGAAAAAA ATCTGCATTT
    CAGTTTGTTT ATCAGGCCTG GATTACTGAT CCTAAAACTG CGCTACGACA GAGGCGCAAG
    GAAAAGAAAA GCTTTCAAAA AGAGGAGAAG CGAAGGCGTA AAGTATATGG GGATGCAGGA
    AGTACTGATG CAGACTGCGA AGACAGTGAA GAAGAACCTG TCAAGAAGAA GTCTGATGGC
    CCAGATAACA TCATCAAGAG GATATTTAAT ATTTTGAAGT TCACTTGGGT CCTTTTCCTG
    GCAACACTAG ACAGTTTTAC AGCTTGGCTT AATTCCATCT CAAGAGAACA CATCGATATC
    TCTACTGTGC TGAGAATCGA ACGTTGTATG CTGACCAGGG AGATTAAGAA GGGAAACGTT
    CCTACCCGGG AGAGCATCCA TATGTATTAC CAGAACCATA TGATGAAACT TTCTAAGGAG
    TCAGGACTGG ACTCTATTGA TAAAAATCCT GGGCAAGCTT CTGGTTTGCA AACATCTGAA
    AGAATGGATA GTTTAGATTC AGCAGCTTCT CGAGACAGTA TCTCCAGCTG CTACACTGAA
    GCAACCATGC TGTTCTCAAG GCAGTCAACC CTTGATGATT TAGATGGACC AGACACTGTT
    CCTAAAACAA GTGAGCGCGC TCGACCTAGG CTTCGTAAAA TGCAGAGCAT GGATATGTCC
    TCTTCATCAG CTGACAGTGG CAGTATAGTG TCAAGCGAGC CTACACAGGT CACCATGCTC
    TATTCTCGTC AGGGGACGAC AGAAACCATT GAGGAGGTAG AAGGAGAGCA TGAAGAGGAA
    GGAGCTCATT CTGCTTCAAG GCAGGATGAA GAAGAGGTGG AAGATTATAG CCTGGGTTCA
    GAAGGAGCTG CATATACTCC CGACACCGAT GTACCATTGT ACTCCACTGT GGACAGCAAT
    GCAGAAGCAC CACCTTCCTA TAGCAAAGCT GTCAGCTTTG AACACCTTCC TTTTGGCTCC
    CCAGATGACT CAGCTGGAAA GAGTCTCATG ATGGTGAGCC CAGATGACAG CCGGACGGAC
    AGACTTAATG ATGCAATTCT CCCTCCATTG ACGCACGAGC TAACGGCTAG TGAGCTGCTT
    CTGAACAAGA TGTTTCATGA TGATGAGCTA GAGGAATCAG AGAAATTCTA TGTTGGTCAG
    CCTCGAGTCT TGCTTCTTAT TTATGCGCTG TACAATACTC TGGTGGCCCG GTCAGAAATG
    GTTTGCTATT TTGTCATCAT CCTTAACCAC ATGATCTCTG CCTCTATGAT AACGCTGGTG
    CTGCCCATCC TTATCTTTCT TTGGGCCATG CTGTCTGTCC CTCGACCAAG CAAACGTTTC
    TGGATGACTG CCATTGTCTA CACTGAGGTA GCCATTGTCA TCAAGTACTT CTTCCAGTTT
    GGCTTCTTTC CTTGGAATAA ATATGTGGAT TACACAAAAG ATAAACCTTA TCATCCACCC
    AATATTATTG GGATAGAGAA GAAAGAGGGC TATGTGCACT ATGATCTTGT TCAGCTCCTT
    GCTTTATTTT TCCACAGATC CATTTTAAAG TGCCATGGAT TGTGGGATGA AGATGAGAAA
    GGGGATAACT CCAGTAATAA AGGAGATACT GATGATGAGC TTTCTCTGCC GGGAGGCAGG
    AGAGACTCTT CTGGGTCTCT GAAGTCGGTC AATCTTGCAG CATCAGTGGA GTCCATCCAT
    GTGCACTTCC CTGAGCAGCA GACAGCAATC AGGAGGAAGA GCTCCAGCAG TGCATCACAG
    CTTTCTCACA GATCCAGCTT CTCCTCACAC AGATCTAAGA GAGGAAGCAC CAGTACAAGG
    AACAGCAGTC AGAAAGGGAG CAGTGTGCTA AGCATCAAGC AAAAATCTAG GAAAGAACTT
    CTTATGGAAA AGTTTCGAGA GCAAATGATC AAAGCCAAAG CCTTTACAAT TAAAAAGACT
    CTCCAAGTGT ATGTGCCCAT CAGACAATTT TTCTACAATC TTATTCATCC AGACTACAGT
    GCAGTGACTG ATGTATATGT GCTGATGTTC CTCGCAGATA CAGTGGACTT CATCATCATT
    GTCTTTGGAT TTTGGGCTTT TGGGAAACAC TCTGCAGCAG CAGATATTAC CTCTTCATTA
    TCAGAGGACC AGGTTCCAGA GGCATTTTTG GTGATGGTGC TCATTCAGTT TGGTACCATG
    GTGGTAGACC GAGCGCTCTA CCTCAAAAAG ACAGTCATGG GAAAGGTCAT CTTCCAGGTC
    ATCCTTGTTT TTGGGATTCA TTTCTGGATG TTCTTTATCT TGCCTGGAGT GACAGAAAGA
    AAATTTAGCC AGAACACCGT TGCCCAGCTC TGGTATTTTG TGAAATGTGT TTATTTTGGC
    TTATCAGCAT ATCAGATACG CTGCGGATAT CCAACTCGTG TTCTTGGCAA CTTCCTCACA
    AAAAGTTATA ACTATGTGAA CCTGTTCTTA TTTCAAGGAT TCCGCCTGGT TCCATTTTTG
    ACTGAGCTGA GAGCAGTAAT GGACTGGGTT TGGACAGATA CCACTCTCAG TCTCTCCAGC
    TGGATCTGTG TTGAAGATAT CTATGCTCAT ATATTCATCC TGAAGTGCTG GCGAGAGTCA
    GAAAAGAGAT ATCCCCAACC AAGAGGCCAG AAGAAAAAGA AGGTTGTGAA GTATGGAATG
    GGTGGCATGA TTATCGTCTT GCTGATTTGT ATTGTCTGGT TCCCACTGCT CTTCATGTCT
    TTAATCAAAT CTGTTGCAGG CATCACCAAC AAGCCTCTAG ATGTATCAAT CACAATAACT
    CTAGGAGGTT ATCAGCCTAT TTTTACAATG AGTGCTCAAC AGAGTCAGCT CAAGGATTTG
    AATCAGACTG GTTTCAGTGC ATTTCTGGGA AGCTATAGGG GTAACACTGC TGCTTTGCAG
    TTTTTAGAGG GCTATGGAAA AGAAGATATA ACCCTAGCAG ATCTAGAAGG GAATTCAAAC
    TCCTTATGGA CCATCAGCCC TCCCAGTAGA GAGAAAATGA TACAGGGACT GCTGGATTTT
    TCAGCTGAGT TCACTGTAGT ACTTTCATGG AGCATTCAAA GAAATCTCAC TCTTGGTGCC
    AAAGCAGAAA TAGCATCAGA TAAACTTACC TTTGGCCTAC CAGAGAAAAC CAGAAGAGAT
    ATTGCTACAA TGATGTCTGG AAAGCCATTG GAAAAGGTAA CATTAGAAAC AGTGTATCCA
    TACTACATCA AGGCTCCTAG TGATTCTCTG GCAAAACCCA TAAAGCAGCT ATTGACAGAC
    TGCAGATGGG AAAATATCAC AGTTTCCCTG GTCAAAAATG TGTCTGAAGA GGGAGTTCGT
    GAGTGGTGGG TTTTGAATCA GCTTGGAAAA AGATACAAAA CAAATGAAGA GAGCCTTGAA
    CTCTTCATAT TCAGTGATAA AGTCAGTCCT CCAAGCCTTG GATTCTTGGC TGGATATGGC
    ATCATGGGAC TATACGCGTC AGTCGTCCTG GTGATAGGGA AGTTCGTCCG TGAGTTTTTC
    AGTGGGATTT CTCACTCCAT CATGTTTGAG GAGCTACCCA ATGTAGACCG AATCTTGAAG
    TTGTGTACTG ACATTTTCTT AGTACGAGAG ACTGGAGAAC TGGAACTAGA AGAAGACCTG
    TACGCCAAAC TAATCTTCCT GTATCGCTCA CCAGAGACTA TGATCAAGTG GACTAGGGAA
    AAAACTAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003590.1
    CDS848..9199
    Translation

    Target ORF information:

    RefSeq Version XM_025147822.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147822.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAAGT TCTGCTGGTA ACAGTGAACG GGAATCCTGC TGACTATCAC
    ACCATCCACC CCTCACTGCC ATTAGAGAAT GGTCCAGCCA AAGCAGACAT GTACTCAACA
    CCACAGTACA AATGGGAGCC CTCGGATAAC ATGTCAGAAA AGGAAGAGGA GGAAGTAGAG
    GAGGAAGAAG AAGTTCCTCA GGAGGAAAAA GAAAATGTTA AATTGCATGC CCTGGTCTCT
    GTGTTCCAAT TTATCATGAA GCAAAGTTAC ATTTGTGCTT TGATAGCTAT GATGGCATGG
    AGTATCACGT ATCACAGCTG GTTGACTTTT GTGCTCTTGA TCTGGTCTTG CACGCTGTGG
    ATGATTCGAG ACCGAAGGAA ATATGCTATG ATCAGTTCAC CGTTCATGGT TTTCTATGGA
    AATTTACTGC TAATATTACA ATATATATGG AGTATTGAGC TCAAGAATGA TGAGCTTCCT
    CAAGTATCAG GTTTTTTAGA AAGAAAGGAA CCTGGAGAGT TGGCATCAAA GATTCTTTTC
    ACGATTACTT TCTGGCTACT GCTTAGGCAA CACCTCACTG AACAGAAAGC ACTTCAGTTA
    AAAGAAGCTA CTTTATCTGA GGTCAAAGTT GGAAATGAAG AAGATGAAGA AAAAGAAGAG
    GGAGAGTTGC AAGAGGGAGA AGTGGCAGAA GAAGAGGAGG AGGAGGAGGA GGAACAGGAA
    GATGATGATG AGGATGAGCA GGATATTATG AAAGTTCTGG GGAAGCTGGT GATGGCTATG
    TTCATCAAGT ACTGGATTTA TGTCTGTGGA GGCATGTTCT TCTTCGTCAG CTTTGAGGGT
    AGAATTGTCA TGTACAAAAT CATCTACATG GTACTATTCC TGTTCTGTGT GGCCCTCTAT
    CAGGTACACT ATGAATGGTG GAGAAAGATT CTGAAATACT TTTGGATGTC AGTTGTTGTT
    TACACGATGT TGGTACTTAT CTTCATCTAC ACATACCAGT TTGAGTCATT CCCTGGGTTG
    TGGAAAAATA TGACAGGCCT GGATGAAAAC AAACTAGCAG ACCTTGGTTT AAAAAGATTT
    TCTGTGGCTG AGCTATTCAC TCGCATCTTT ATACCCACCT CCTTCCTACT GGCGTGTATC
    TTGCACCTTC ACTATTTCCA TGACCGTTTC CTCCAGCTCA CTGATTTAAA AGCTGTAACC
    AGCAAACAGG ACAACACTAT TTACAGACTG GTGCATCAAG ATGGAAGCCT GCCTGACATA
    ACTATGATGA ATTTAACAGC CAGCAGTGAA AAGGAAGAGG ATAAAATGCT GAAAGAGGCT
    GGTGAGAAAA GAATGGAAGA GCCAGATGGG GAGGGAGGGA AAGGGAGGGC TGAAAAAGGA
    AAAGAAGAAG AAGAGGAAGA TGAGGAGAAG GAAGATGATG ATGTAGACGA TGAGTCAGAG
    GAAGAAGAAA CTACAGACCT AAGAAACAAG TGGCATCTGG TGATTGACCG CCTTACAGTG
    CTGTTTTTGA AATTCCTGGA GTACTTTCAT AAGATGCAAG TCTTTGTCTG GTGGCTTTTG
    GAGCTGCATA TCATCAAAAT CGTCTCCTCT TACATCATCT GGGTCACAGT AAAAGAGGTG
    TCTCTGTTTA ACTTTGTGTT TTTAATTGCT TGGGCTCTTG CTTTGCCATA TGCTCAGTTT
    CGCCCATTGG CATCAAGTAT CTGCACTGTT TGGACATGTG TGATTATTGT CTGCAAAATG
    TTGTACCAGC TCACATCTAT TGATCCCAGC ACTTTTTCCA GTAACTGTAC ATTGCCCAGA
    GAAAATGAAA CAAAGGTTGA TTTAGAAGAG CTCAAAACAT CAGTCCTCTA CAGTGGTCCG
    GTTGATCCTG CAGAATGGGT TGGATTGAGG AAGTCTTATC CCCTACTTTT ATACTTAAGG
    AATAACTTAC TGATGCTGGC TATTCTGGCT TTTGAAGTTA CAATTTACCG TCATCAAGAG
    TACTACAGAT GTCGAAATAA CCTTACTGCA CCTGTAACTA AAACTATTTT CCATGATATT
    ACAAGAGCAC ATCTGGATGA TGGACTGGTA AACTGTGTCA AGTATTTCAT AAACTACTTC
    TTCTACAAGT TTGGTTTAGA GACCTGTTTT CTTTTATCAG TGAATGTAAT TGGTCAACGG
    ATGGATTTTT ATGCCATGAT TCATGCCTTC TGGTTGATTG CTGTATTGTA TCGACGTAGA
    AGAAAAGCTA TTGCAGAGAT CTGGCCAAAG TATTGCTGTT TTCTGGCATG CATCATTACA
    TTCCAGTATT TCCTTTGTAT TGGCATTCCA CCTGCTCCTT GTAAAGACTA TCCATGGAGA
    AGTGGTAATG CCAACTTCAA CTCAAATATC ATAAAGTGGT TATACTTTCC AGACTTCATT
    GTGAGACCAA ACCCTGTCTT CCTTGTCTAT GATTTCATGT TGCTGCTGTG TGCATCCTTA
    CAAAGGCAAA CGTTTGAGGA TGAAAATAAA GCTGCTGTGC GAATCATGGC TGGAGACAAC
    GTGGAAATTT GTATGAATCT TGAAGCAGCA TCTTTTAGCC AGCATAATCC TGTTCCAGAC
    TTCATTCACT GCCGGTCCTA CTTAGATATG TATAAGGTGA TAATCTTTAG TTACCTCTTC
    TGGTTTGTGC TTACTATAAT CTTCATAACG GGAACAACGA GGATCAGCAT ATTCTGTATG
    GGCTACTTGG TTGCCTGCTT TTATTTCCTT CTCTTTGGTG GAGATCTGCT GCTGAAACCC
    ATCAGGAGCA TCCTGCGCTA CTGGGACTGG CTGATTGCAT ACAATGTTTT TGTGATCACC
    ATGAAGAACA TCCTCTCGAT AGGAGCATGT GGCTACATTG AGTCTTTAAT TCAAAATAGT
    TGCTGGTTGA TTCAGGCATT TAGTCTGGCT TGCACAGTTA AAGGCTACCG TATCCCAACC
    AACAATGCAG ACTGTAAACT ACCTAGTGGA GAAGCAGGAA TCATCTGGGA CAGTATATGT
    TTTGCCTTTC TACTGCTGCA AAGAAGAGTC TTCATGAGTT ACTACTTCCT CCACGTGGTT
    GCAGATATAA AAGCTTCTCA GATCCTAGCA TCAAGGGGTG CTGAGCTTTT CCAGGCCACA
    ATTGTTAAGG CAGTAAAGGC AAGAATTGAA GAGGAAAAAA AATCAATGGA TCAACTGAAA
    AGACAAATGG ATCGCATCAA AGCCAGGCAG CAAAAATATA AAAAGGGTAA AGAGAGGATG
    CTAAGCATGA CACAGGATTC CACAGAGGGG CCTGAAATTC GGAAGGTTTC GGAAGAAGAT
    GATGAAGGAG AAGCAGACAA AGAGAAAGCC AAGGGCAAAA AAAAGCTGTG GTGGCGGCCT
    TGGGTTGATC ATGCTTCCAT GGTCAGAAGT GGAAATTATT ATTTGTTTGA GACGGACAGT
    GAAGAGGAAG AGGAAGAAGA GAAAAAAGAA GAGGAAGAGC CTCGAAAAAA ATCTGCATTT
    CAGTTTGTTT ATCAGGCCTG GATTACTGAT CCTAAAACTG CGCTACGACA GAGGCGCAAG
    GAAAAGAAAA GCTTTCAAAA AGAGGAGAAG CGAAGGCGTA AAGTATATGG GGATGCAGGA
    AGTACTGATG CAGACTGCGA AGACAGTGAA GAAGAACCTG TCAAGAAGAA GTCTGATGGC
    CCAGATAACA TCATCAAGAG GATATTTAAT ATTTTGAAGT TCACTTGGGT CCTTTTCCTG
    GCAACACTAG ACAGTTTTAC AGCTTGGCTT AATTCCATCT CAAGAGAACA CATCGATATC
    TCTACTGTGC TGAGAATCGA ACGTTGTATG CTGACCAGGG AGATTAAGAA GGGAAACGTT
    CCTACCCGGG AGAGCATCCA TATGTATTAC CAGAACCATA TGATGAAACT TTCTAAGGAG
    TCAGGACTGG ACTCTATTGA TAAAAATCCT GGGCAAGCTT CTGGTTTGCA AACATCTGAA
    AGAATGGATA GTTTAGATTC AGCAGCTTCT CGAGACAGTA TCTCCAGCTG CTACACTGAA
    GCAACCATGC TGTTCTCAAG GCAGTCAACC CTTGATGATT TAGATGGACC AGACACTGTT
    CCTAAAACAA GTGAGCGCGC TCGACCTAGG CTTCGTAAAA TGCAGAGCAT GGATATGTCC
    TCTTCATCAG CTGACAGTGG CAGTATAGTG TCAAGCGAGC CTACACAGGT CACCATGCTC
    TATTCTCGTC AGGGGACGAC AGAAACCATT GAGGAGGTAG AAGGAGAGCA TGAAGAGGAA
    GGAGCTCATT CTGCTTCAAG GCAGGATGAA GAAGAGGTGG AAGATTATAG CCTGGGTTCA
    GAAGGAGCTG CATATACTCC CGACACCGAT GTACCATTGT ACTCCACTGT GGACAGCAAT
    GCAGAAGCAC CACCTTCCTA TAGCAAAGCT GTCAGCTTTG AACACCTTCC TTTTGGCTCC
    CCAGATGACT CAGCTGGAAA GAGTCTCATG ATGGTGAGCC CAGATGACAG CCGGACGGAC
    AGACTTAATG ATGCAATTCT CCCTCCATTG ACGCACGAGC TAACGGCTAG TGAGCTGCTT
    CTGAACAAGA TGTTTCATGA TGATGAGCTA GAGGAATCAG AGAAATTCTA TGTTGGTCAG
    CCTCGAGTCT TGCTTCTTAT TTATGCGCTG TACAATACTC TGGTGGCCCG GTCAGAAATG
    GTTTGCTATT TTGTCATCAT CCTTAACCAC ATGATCTCTG CCTCTATGAT AACGCTGGTG
    CTGCCCATCC TTATCTTTCT TTGGGCCATG CTGTCTGTCC CTCGACCAAG CAAACGTTTC
    TGGATGACTG CCATTGTCTA CACTGAGGTA GCCATTGTCA TCAAGTACTT CTTCCAGTTT
    GGCTTCTTTC CTTGGAATAA ATATGTGGAT TACACAAAAG ATAAACCTTA TCATCCACCC
    AATATTATTG GGATAGAGAA GAAAGAGGGC TATGTGCACT ATGATCTTGT TCAGCTCCTT
    GCTTTATTTT TCCACAGATC CATTTTAAAG TGCCATGGAT TGTGGGATGA AGATGAGAAA
    GGGGATAACT CCAGTAATAA AGGAGATACT GATGATGAGC TTTCTCTGCC GGGAGGCAGG
    AGAGACTCTT CTGGGTCTCT GAAGTCGGTC AATCTTGCAG CATCAGTGGA GTCCATCCAT
    GTGCACTTCC CTGAGCAGCA GACAGCAATC AGGAGGAAGA GCTCCAGCAG TGCATCACAG
    CTTTCTCACA GATCCAGCTT CTCCTCACAC AGATCTAAGA GAGGAAGCAC CAGTACAAGG
    AACAGCAGTC AGAAAGGGAG CAGTGTGCTA AGCATCAAGC AAAAATCTAG GAAAGAACTT
    CTTATGGAAA AGTTTCGAGA GCAAATGATC AAAGCCAAAG CCTTTACAAT TAAAAAGACT
    CTCCAAGTGT ATGTGCCCAT CAGACAATTT TTCTACAATC TTATTCATCC AGACTACAGT
    GCAGTGACTG ATGTATATGT GCTGATGTTC CTCGCAGATA CAGTGGACTT CATCATCATT
    GTCTTTGGAT TTTGGGCTTT TGGGAAACAC TCTGCAGCAG CAGATATTAC CTCTTCATTA
    TCAGAGGACC AGGTTCCAGA GGCATTTTTG GTGATGGTGC TCATTCAGTT TGGTACCATG
    GTGGTAGACC GAGCGCTCTA CCTCAAAAAG ACAGTCATGG GAAAGGTCAT CTTCCAGGTC
    ATCCTTGTTT TTGGGATTCA TTTCTGGATG TTCTTTATCT TGCCTGGAGT GACAGAAAGA
    AAATTTAGCC AGAACACCGT TGCCCAGCTC TGGTATTTTG TGAAATGTGT TTATTTTGGC
    TTATCAGCAT ATCAGATACG CTGCGGATAT CCAACTCGTG TTCTTGGCAA CTTCCTCACA
    AAAAGTTATA ACTATGTGAA CCTGTTCTTA TTTCAAGGAT TCCGCCTGGT TCCATTTTTG
    ACTGAGCTGA GAGCAGTAAT GGACTGGGTT TGGACAGATA CCACTCTCAG TCTCTCCAGC
    TGGATCTGTG TTGAAGATAT CTATGCTCAT ATATTCATCC TGAAGTGCTG GCGAGAGTCA
    GAAAAGAGAT ATCCCCAACC AAGAGGCCAG AAGAAAAAGA AGGTTGTGAA GTATGGAATG
    GGTGGCATGA TTATCGTCTT GCTGATTTGT ATTGTCTGGT TCCCACTGCT CTTCATGTCT
    TTAATCAAAT CTGTTGCAGG CATCACCAAC AAGCCTCTAG ATGTATCAAT CACAATAACT
    CTAGGAGGTT ATCAGCCTAT TTTTACAATG AGTGCTCAAC AGAGTCAGCT CAAGGATTTG
    AATCAGACTG GTTTCAGTGC ATTTCTGGGA AGCTATAGGG GTAACACTGC TGCTTTGCAG
    TTTTTAGAGG GCTATGGAAA AGAAGATATA ACCCTAGCAG ATCTAGAAGG GAATTCAAAC
    TCCTTATGGA CCATCAGCCC TCCCAGTAGA GAGAAAATGA TACAGGGACT GCTGGATTTT
    TCAGCTGAGT TCACTGTAGT ACTTTCATGG AGCATTCAAA GAAATCTCAC TCTTGGTGCC
    AAAGCAGAAA TAGCATCAGA TAAACTTACC TTTGGCCTAC CAGAGAAAAC CAGAAGAGAT
    ATTGCTACAA TGATGTCTGG AAAGCCATTG GAAAAGGTAA CATTAGAAAC AGTGTATCCA
    TACTACATCA AGGCTCCTAG TGATTCTCTG GCAAAACCCA TAAAGCAGCT ATTGACAGAC
    TGCAGATGGG AAAATATCAC AGTTTCCCTG GTCAAAAATG TGTCTGAAGA GGGAGTTCGT
    GAGTGGTGGG TTTTGAATCA GCTTGGAAAA AGATACAAAA CAAATGAAGA GAGCCTTGAA
    CTCTTCATAT TCAGTGATAA AGTCAGTCCT CCAAGCCTTG GATTCTTGGC TGGATATGGC
    ATCATGGGAC TATACGCGTC AGTCGTCCTG GTGATAGGGA AGTTCGTCCG TGAGTTTTTC
    AGTGGGATTT CTCACTCCAT CATGTTTGAG GAGCTACCCA ATGTAGACCG AATCTTGAAG
    TTGTGTACTG ACATTTTCTT AGTACGAGAG ACTGGAGAAC TGGAACTAGA AGAAGACCTG
    TACGCCAAAC TAATCTTCCT GTATCGCTCA CCAGAGACTA TGATCAAGTG GACTAGGGAA
    AAAACTAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146282
    Clone ID Related Accession (Same CDS sequence) XM_025147824.1
    Accession Version XM_025147824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGCAGGAA GTACTGATGC AGACTGCGAA
    GACAGTGAAG AAGAACCTGT CAAGAAGAAG TCTGATGGCC CAGATAACAT CATCAAGAGG
    ATATTTAATA TTTTGAAGTT CACTTGGGTC CTTTTCCTGG CAACACTAGA CAGTTTTACA
    GCTTGGCTTA ATTCCATCTC AAGAGAACAC ATCGATATCT CTACTGTGCT GAGAATCGAA
    CGTTGTATGC TGACCAGGGA GATTAAGAAG GGAAACGTTC CTACCCGGGA GAGCATCCAT
    ATGTATTACC AGAACCATAT GATGAAACTT TCTAAGGAGT CAGGACTGGA CTCTATTGAT
    AAAAATCCTG GGCAAGCTTC TGGTTTGCAA ACATCTGAAA GAATGGATAG TTTAGATTCA
    GCAGCTTCTC GAGACAGTAT CTCCAGCGAG CCTACACAGG TCACCATGCT CTATTCTCGT
    CAGGGGACGA CAGAAACCAT TGAGGAGGTA GAAGGAGAGC ATGAAGAGGA AGGAGCTCAT
    TCTGCTTCAA GGCAGGATGA AGAAGAGGTG GAAGATTATA GCCTGGGTTC AGAAGGAGCT
    GCATATACTC CCGACACCGA TGTACCATTG TACTCCACTG TGGACAGCAA TGCAGAAGCA
    CCACCTTCCT ATAGCAAAGC TGTCAGCTTT GAACACCTTC CTTTTGGCTC CCCAGATGAC
    TCAGCTGGAA AGAGTCTCAT GATGGTGAGC CCAGATGACA GCCGGACGGA CAGACTTAAT
    GATGCAATTC TCCCTCCATT GACGCACGAG CTAACGGCTA GTGAGCTGCT TCTGAACAAG
    ATGTTTCATG ATGATGAGCT AGAGGAATCA GAGAAATTCT ATGTTGGTCA GCCTCGAGTC
    TTGCTTCTTA TTTATGCGCT GTACAATACT CTGGTGGCCC GGTCAGAAAT GGTTTGCTAT
    TTTGTCATCA TCCTTAACCA CATGATCTCT GCCTCTATGA TAACGCTGGT GCTGCCCATC
    CTTATCTTTC TTTGGGCCAT GCTGTCTGTC CCTCGACCAA GCAAACGTTT CTGGATGACT
    GCCATTGTCT ACACTGAGGT AGCCATTGTC ATCAAGTACT TCTTCCAGTT TGGCTTCTTT
    CCTTGGAATA AATATGTGGA TTACACAAAA GATAAACCTT ATCATCCACC CAATATTATT
    GGGATAGAGA AGAAAGAGGG CTATGTGCAC TATGATCTTG TTCAGCTCCT TGCTTTATTT
    TTCCACAGAT CCATTTTAAA GTGCCATGGA TTGTGGGATG AAGATGAGAA AGGGGATAAC
    TCCAGTAATA AAGGAGATAC TGATGATGAG CTTTCTCTGC CGGGAGGCAG GAGAGACTCT
    TCTGGGTCTC TGAAGTCGGT CAATCTTGCA GCATCAGTGG AGTCCATCCA TGTGCACTTC
    CCTGAGCAGC AGACAGCAAT CAGGAGGAAG AGCTCCAGCA GTGCATCACA GCTTTCTCAC
    AGATCCAGCT TCTCCTCACA CAGATCTAAG AGAGGAAGCA CCAGTACAAG GAACAGCAGT
    CAGAAAGGGA GCAGTGTGCT AAGCATCAAG CAAAAATCTA GGAAAGAACT TCTTATGGAA
    AAGTTTCGAG AGCAAATGAT CAAAGCCAAA GCCTTTACAA TTAAAAAGAC TCTCCAAGTG
    TATGTGCCCA TCAGACAATT TTTCTACAAT CTTATTCATC CAGACTACAG TGCAGTGACT
    GATGTATATG TGCTGATGTT CCTCGCAGAT ACAGTGGACT TCATCATCAT TGTCTTTGGA
    TTTTGGGCTT TTGGGAAACA CTCTGCAGCA GCAGATATTA CCTCTTCATT ATCAGAGGAC
    CAGGTTCCAG AGGCATTTTT GGTGATGGTG CTCATTCAGT TTGGTACCAT GGTGGTAGAC
    CGAGCGCTCT ACCTCAAAAA GACAGTCATG GGAAAGGTCA TCTTCCAGGT CATCCTTGTT
    TTTGGGATTC ATTTCTGGAT GTTCTTTATC TTGCCTGGAG TGACAGAAAG AAAATTTAGC
    CAGAACACCG TTGCCCAGCT CTGGTATTTT GTGAAATGTG TTTATTTTGG CTTATCAGCA
    TATCAGATAC GCTGCGGATA TCCAACTCGT GTTCTTGGCA ACTTCCTCAC AAAAAGTTAT
    AACTATGTGA ACCTGTTCTT ATTTCAAGGA TTCCGCCTGG TTCCATTTTT GACTGAGCTG
    AGAGCAGTAA TGGACTGGGT TTGGACAGAT ACCACTCTCA GTCTCTCCAG CTGGATCTGT
    GTTGAAGATA TCTATGCTCA TATATTCATC CTGAAGTGCT GGCGAGAGTC AGAAAAGAGA
    TATCCCCAAC CAAGAGGCCA GAAGAAAAAG AAGGTTGTGA AGTATGGAAT GGGTGGCATG
    ATTATCGTCT TGCTGATTTG TATTGTCTGG TTCCCACTGC TCTTCATGTC TTTAATCAAA
    TCTGTTGCAG GCATCACCAA CAAGCCTCTA GATGTATCAA TCACAATAAC TCTAGGAGGT
    TATCAGCCTA TTTTTACAAT GAGTGCTCAA CAGAGTCAGC TCAAGGATTT GAATCAGACT
    GGTTTCAGTG CATTTCTGGG AAGCTATAGG GGTAACACTG CTGCTTTGCA GTTTTTAGAG
    GGCTATGGAA AAGAAGATAT AACCCTAGCA GATCTAGAAG GGAATTCAAA CTCCTTATGG
    ACCATCAGCC CTCCCAGTAG AGAGAAAATG ATACAGGGAC TGCTGGATTT TTCAGCTGAG
    TTCACTGTAG TACTTTCATG GAGCATTCAA AGAAATCTCA CTCTTGGTGC CAAAGCAGAA
    ATAGCATCAG ATAAACTTAC CTTTGGCCTA CCAGAGAAAA CCAGAAGAGA TATTGCTACA
    ATGATGTCTG GAAAGCCATT GGAAAAGGTA ACATTAGAAA CAGTGTATCC ATACTACATC
    AAGGCTCCTA GTGATTCTCT GGCAAAACCC ATAAAGCAGC TATTGACAGA CTGCAGATGG
    GAAAATATCA CAGTTTCCCT GGTCAAAAAT GTGTCTGAAG AGGGAGTTCG TGAGTGGTGG
    GTTTTGAATC AGCTTGGAAA AAGATACAAA ACAAATGAAG AGAGCCTTGA ACTCTTCATA
    TTCAGTGATA AAGTCAGTCC TCCAAGCCTT GGATTCTTGG CTGGATATGG CATCATGGGA
    CTATACGCGT CAGTCGTCCT GGTGATAGGG AAGTTCGTCC GTGAGTTTTT CAGTGGGATT
    TCTCACTCCA TCATGTTTGA GGAGCTACCC AATGTAGACC GAATCTTGAA GTTGTGTACT
    GACATTTTCT TAGTACGAGA GACTGGAGAA CTGGAACTAG AAGAAGACCT GTACGCCAAA
    CTAATCTTCC TGTATCGCTC ACCAGAGACT ATGATCAAGT GGACTAGGGA AAAAACTAAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003592.1
    CDS848..9070
    Translation

    Target ORF information:

    RefSeq Version XM_025147824.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGCAGGAA GTACTGATGC AGACTGCGAA
    GACAGTGAAG AAGAACCTGT CAAGAAGAAG TCTGATGGCC CAGATAACAT CATCAAGAGG
    ATATTTAATA TTTTGAAGTT CACTTGGGTC CTTTTCCTGG CAACACTAGA CAGTTTTACA
    GCTTGGCTTA ATTCCATCTC AAGAGAACAC ATCGATATCT CTACTGTGCT GAGAATCGAA
    CGTTGTATGC TGACCAGGGA GATTAAGAAG GGAAACGTTC CTACCCGGGA GAGCATCCAT
    ATGTATTACC AGAACCATAT GATGAAACTT TCTAAGGAGT CAGGACTGGA CTCTATTGAT
    AAAAATCCTG GGCAAGCTTC TGGTTTGCAA ACATCTGAAA GAATGGATAG TTTAGATTCA
    GCAGCTTCTC GAGACAGTAT CTCCAGCGAG CCTACACAGG TCACCATGCT CTATTCTCGT
    CAGGGGACGA CAGAAACCAT TGAGGAGGTA GAAGGAGAGC ATGAAGAGGA AGGAGCTCAT
    TCTGCTTCAA GGCAGGATGA AGAAGAGGTG GAAGATTATA GCCTGGGTTC AGAAGGAGCT
    GCATATACTC CCGACACCGA TGTACCATTG TACTCCACTG TGGACAGCAA TGCAGAAGCA
    CCACCTTCCT ATAGCAAAGC TGTCAGCTTT GAACACCTTC CTTTTGGCTC CCCAGATGAC
    TCAGCTGGAA AGAGTCTCAT GATGGTGAGC CCAGATGACA GCCGGACGGA CAGACTTAAT
    GATGCAATTC TCCCTCCATT GACGCACGAG CTAACGGCTA GTGAGCTGCT TCTGAACAAG
    ATGTTTCATG ATGATGAGCT AGAGGAATCA GAGAAATTCT ATGTTGGTCA GCCTCGAGTC
    TTGCTTCTTA TTTATGCGCT GTACAATACT CTGGTGGCCC GGTCAGAAAT GGTTTGCTAT
    TTTGTCATCA TCCTTAACCA CATGATCTCT GCCTCTATGA TAACGCTGGT GCTGCCCATC
    CTTATCTTTC TTTGGGCCAT GCTGTCTGTC CCTCGACCAA GCAAACGTTT CTGGATGACT
    GCCATTGTCT ACACTGAGGT AGCCATTGTC ATCAAGTACT TCTTCCAGTT TGGCTTCTTT
    CCTTGGAATA AATATGTGGA TTACACAAAA GATAAACCTT ATCATCCACC CAATATTATT
    GGGATAGAGA AGAAAGAGGG CTATGTGCAC TATGATCTTG TTCAGCTCCT TGCTTTATTT
    TTCCACAGAT CCATTTTAAA GTGCCATGGA TTGTGGGATG AAGATGAGAA AGGGGATAAC
    TCCAGTAATA AAGGAGATAC TGATGATGAG CTTTCTCTGC CGGGAGGCAG GAGAGACTCT
    TCTGGGTCTC TGAAGTCGGT CAATCTTGCA GCATCAGTGG AGTCCATCCA TGTGCACTTC
    CCTGAGCAGC AGACAGCAAT CAGGAGGAAG AGCTCCAGCA GTGCATCACA GCTTTCTCAC
    AGATCCAGCT TCTCCTCACA CAGATCTAAG AGAGGAAGCA CCAGTACAAG GAACAGCAGT
    CAGAAAGGGA GCAGTGTGCT AAGCATCAAG CAAAAATCTA GGAAAGAACT TCTTATGGAA
    AAGTTTCGAG AGCAAATGAT CAAAGCCAAA GCCTTTACAA TTAAAAAGAC TCTCCAAGTG
    TATGTGCCCA TCAGACAATT TTTCTACAAT CTTATTCATC CAGACTACAG TGCAGTGACT
    GATGTATATG TGCTGATGTT CCTCGCAGAT ACAGTGGACT TCATCATCAT TGTCTTTGGA
    TTTTGGGCTT TTGGGAAACA CTCTGCAGCA GCAGATATTA CCTCTTCATT ATCAGAGGAC
    CAGGTTCCAG AGGCATTTTT GGTGATGGTG CTCATTCAGT TTGGTACCAT GGTGGTAGAC
    CGAGCGCTCT ACCTCAAAAA GACAGTCATG GGAAAGGTCA TCTTCCAGGT CATCCTTGTT
    TTTGGGATTC ATTTCTGGAT GTTCTTTATC TTGCCTGGAG TGACAGAAAG AAAATTTAGC
    CAGAACACCG TTGCCCAGCT CTGGTATTTT GTGAAATGTG TTTATTTTGG CTTATCAGCA
    TATCAGATAC GCTGCGGATA TCCAACTCGT GTTCTTGGCA ACTTCCTCAC AAAAAGTTAT
    AACTATGTGA ACCTGTTCTT ATTTCAAGGA TTCCGCCTGG TTCCATTTTT GACTGAGCTG
    AGAGCAGTAA TGGACTGGGT TTGGACAGAT ACCACTCTCA GTCTCTCCAG CTGGATCTGT
    GTTGAAGATA TCTATGCTCA TATATTCATC CTGAAGTGCT GGCGAGAGTC AGAAAAGAGA
    TATCCCCAAC CAAGAGGCCA GAAGAAAAAG AAGGTTGTGA AGTATGGAAT GGGTGGCATG
    ATTATCGTCT TGCTGATTTG TATTGTCTGG TTCCCACTGC TCTTCATGTC TTTAATCAAA
    TCTGTTGCAG GCATCACCAA CAAGCCTCTA GATGTATCAA TCACAATAAC TCTAGGAGGT
    TATCAGCCTA TTTTTACAAT GAGTGCTCAA CAGAGTCAGC TCAAGGATTT GAATCAGACT
    GGTTTCAGTG CATTTCTGGG AAGCTATAGG GGTAACACTG CTGCTTTGCA GTTTTTAGAG
    GGCTATGGAA AAGAAGATAT AACCCTAGCA GATCTAGAAG GGAATTCAAA CTCCTTATGG
    ACCATCAGCC CTCCCAGTAG AGAGAAAATG ATACAGGGAC TGCTGGATTT TTCAGCTGAG
    TTCACTGTAG TACTTTCATG GAGCATTCAA AGAAATCTCA CTCTTGGTGC CAAAGCAGAA
    ATAGCATCAG ATAAACTTAC CTTTGGCCTA CCAGAGAAAA CCAGAAGAGA TATTGCTACA
    ATGATGTCTG GAAAGCCATT GGAAAAGGTA ACATTAGAAA CAGTGTATCC ATACTACATC
    AAGGCTCCTA GTGATTCTCT GGCAAAACCC ATAAAGCAGC TATTGACAGA CTGCAGATGG
    GAAAATATCA CAGTTTCCCT GGTCAAAAAT GTGTCTGAAG AGGGAGTTCG TGAGTGGTGG
    GTTTTGAATC AGCTTGGAAA AAGATACAAA ACAAATGAAG AGAGCCTTGA ACTCTTCATA
    TTCAGTGATA AAGTCAGTCC TCCAAGCCTT GGATTCTTGG CTGGATATGG CATCATGGGA
    CTATACGCGT CAGTCGTCCT GGTGATAGGG AAGTTCGTCC GTGAGTTTTT CAGTGGGATT
    TCTCACTCCA TCATGTTTGA GGAGCTACCC AATGTAGACC GAATCTTGAA GTTGTGTACT
    GACATTTTCT TAGTACGAGA GACTGGAGAA CTGGAACTAG AAGAAGACCT GTACGCCAAA
    CTAATCTTCC TGTATCGCTC ACCAGAGACT ATGATCAAGT GGACTAGGGA AAAAACTAAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146283
    Clone ID Related Accession (Same CDS sequence) XM_015282543.2
    Accession Version XM_015282543.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGGAAGTA CTGATGCAGA CTGCGAAGAC
    AGTGAAGAAG AACCTGTCAA GAAGAAGTCT GATGGCCCAG ATAACATCAT CAAGAGGATA
    TTTAATATTT TGAAGTTCAC TTGGGTCCTT TTCCTGGCAA CACTAGACAG TTTTACAGCT
    TGGCTTAATT CCATCTCAAG AGAACACATC GATATCTCTA CTGTGCTGAG AATCGAACGT
    TGTATGCTGA CCAGGGAGAT TAAGAAGGGA AACGTTCCTA CCCGGGAGAG CATCCATATG
    TATTACCAGA ACCATATGAT GAAACTTTCT AAGGAGTCAG GACTGGACTC TATTGATAAA
    AATCCTGGGC AAGCTTCTGG TTTGCAAACA TCTGAAAGAA TGGATAGTTT AGATTCAGCA
    GCTTCTCGAG ACAGTATCTC CAGCGAGCCT ACACAGGTCA CCATGCTCTA TTCTCGTCAG
    GGGACGACAG AAACCATTGA GGAGGTAGAA GGAGAGCATG AAGAGGAAGG AGCTCATTCT
    GCTTCAAGGC AGGATGAAGA AGAGGTGGAA GATTATAGCC TGGGTTCAGA AGGAGCTGCA
    TATACTCCCG ACACCGATGT ACCATTGTAC TCCACTGTGG ACAGCAATGC AGAAGCACCA
    CCTTCCTATA GCAAAGCTGT CAGCTTTGAA CACCTTCCTT TTGGCTCCCC AGATGACTCA
    GCTGGAAAGA GTCTCATGAT GGTGAGCCCA GATGACAGCC GGACGGACAG ACTTAATGAT
    GCAATTCTCC CTCCATTGAC GCACGAGCTA ACGGCTAGTG AGCTGCTTCT GAACAAGATG
    TTTCATGATG ATGAGCTAGA GGAATCAGAG AAATTCTATG TTGGTCAGCC TCGAGTCTTG
    CTTCTTATTT ATGCGCTGTA CAATACTCTG GTGGCCCGGT CAGAAATGGT TTGCTATTTT
    GTCATCATCC TTAACCACAT GATCTCTGCC TCTATGATAA CGCTGGTGCT GCCCATCCTT
    ATCTTTCTTT GGGCCATGCT GTCTGTCCCT CGACCAAGCA AACGTTTCTG GATGACTGCC
    ATTGTCTACA CTGAGGTAGC CATTGTCATC AAGTACTTCT TCCAGTTTGG CTTCTTTCCT
    TGGAATAAAT ATGTGGATTA CACAAAAGAT AAACCTTATC ATCCACCCAA TATTATTGGG
    ATAGAGAAGA AAGAGGGCTA TGTGCACTAT GATCTTGTTC AGCTCCTTGC TTTATTTTTC
    CACAGATCCA TTTTAAAGTG CCATGGATTG TGGGATGAAG ATGAGAAAGG GGATAACTCC
    AGTAATAAAG GAGATACTGA TGATGAGCTT TCTCTGCCGG GAGGCAGGAG AGACTCTTCT
    GGGTCTCTGA AGTCGGTCAA TCTTGCAGCA TCAGTGGAGT CCATCCATGT GCACTTCCCT
    GAGCAGCAGA CAGCAATCAG GAGGAAGAGC TCCAGCAGTG CATCACAGCT TTCTCACAGA
    TCCAGCTTCT CCTCACACAG ATCTAAGAGA GGAAGCACCA GTACAAGGAA CAGCAGTCAG
    AAAGGGAGCA GTGTGCTAAG CATCAAGCAA AAATCTAGGA AAGAACTTCT TATGGAAAAG
    TTTCGAGAGC AAATGATCAA AGCCAAAGCC TTTACAATTA AAAAGACTCT CCAAGTGTAT
    GTGCCCATCA GACAATTTTT CTACAATCTT ATTCATCCAG ACTACAGTGC AGTGACTGAT
    GTATATGTGC TGATGTTCCT CGCAGATACA GTGGACTTCA TCATCATTGT CTTTGGATTT
    TGGGCTTTTG GGAAACACTC TGCAGCAGCA GATATTACCT CTTCATTATC AGAGGACCAG
    GTTCCAGAGG CATTTTTGGT GATGGTGCTC ATTCAGTTTG GTACCATGGT GGTAGACCGA
    GCGCTCTACC TCAAAAAGAC AGTCATGGGA AAGGTCATCT TCCAGGTCAT CCTTGTTTTT
    GGGATTCATT TCTGGATGTT CTTTATCTTG CCTGGAGTGA CAGAAAGAAA ATTTAGCCAG
    AACACCGTTG CCCAGCTCTG GTATTTTGTG AAATGTGTTT ATTTTGGCTT ATCAGCATAT
    CAGATACGCT GCGGATATCC AACTCGTGTT CTTGGCAACT TCCTCACAAA AAGTTATAAC
    TATGTGAACC TGTTCTTATT TCAAGGATTC CGCCTGGTTC CATTTTTGAC TGAGCTGAGA
    GCAGTAATGG ACTGGGTTTG GACAGATACC ACTCTCAGTC TCTCCAGCTG GATCTGTGTT
    GAAGATATCT ATGCTCATAT ATTCATCCTG AAGTGCTGGC GAGAGTCAGA AAAGAGATAT
    CCCCAACCAA GAGGCCAGAA GAAAAAGAAG GTTGTGAAGT ATGGAATGGG TGGCATGATT
    ATCGTCTTGC TGATTTGTAT TGTCTGGTTC CCACTGCTCT TCATGTCTTT AATCAAATCT
    GTTGCAGGCA TCACCAACAA GCCTCTAGAT GTATCAATCA CAATAACTCT AGGAGGTTAT
    CAGCCTATTT TTACAATGAG TGCTCAACAG AGTCAGCTCA AGGATTTGAA TCAGACTGGT
    TTCAGTGCAT TTCTGGGAAG CTATAGGGGT AACACTGCTG CTTTGCAGTT TTTAGAGGGC
    TATGGAAAAG AAGATATAAC CCTAGCAGAT CTAGAAGGGA ATTCAAACTC CTTATGGACC
    ATCAGCCCTC CCAGTAGAGA GAAAATGATA CAGGGACTGC TGGATTTTTC AGCTGAGTTC
    ACTGTAGTAC TTTCATGGAG CATTCAAAGA AATCTCACTC TTGGTGCCAA AGCAGAAATA
    GCATCAGATA AACTTACCTT TGGCCTACCA GAGAAAACCA GAAGAGATAT TGCTACAATG
    ATGTCTGGAA AGCCATTGGA AAAGGTAACA TTAGAAACAG TGTATCCATA CTACATCAAG
    GCTCCTAGTG ATTCTCTGGC AAAACCCATA AAGCAGCTAT TGACAGACTG CAGATGGGAA
    AATATCACAG TTTCCCTGGT CAAAAATGTG TCTGAAGAGG GAGTTCGTGA GTGGTGGGTT
    TTGAATCAGC TTGGAAAAAG ATACAAAACA AATGAAGAGA GCCTTGAACT CTTCATATTC
    AGTGATAAAG TCAGTCCTCC AAGCCTTGGA TTCTTGGCTG GATATGGCAT CATGGGACTA
    TACGCGTCAG TCGTCCTGGT GATAGGGAAG TTCGTCCGTG AGTTTTTCAG TGGGATTTCT
    CACTCCATCA TGTTTGAGGA GCTACCCAAT GTAGACCGAA TCTTGAAGTT GTGTACTGAC
    ATTTTCTTAG TACGAGAGAC TGGAGAACTG GAACTAGAAG AAGACCTGTA CGCCAAACTA
    ATCTTCCTGT ATCGCTCACC AGAGACTATG ATCAAGTGGA CTAGGGAAAA AACTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138029.2
    CDS848..9067
    Translation

    Target ORF information:

    RefSeq Version XM_015282543.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282543.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGTCACGTA AGGAGAAGGA GAACCCCCAG GAGGAGCGCG GCGGGCGGAC CCCAGGCTCT 
    ACGGAGCATC GAGCCTGTGC TTTCCGTTAC AATGGACTCT CCTTTGTCTA CCTCATCTAC
    CTACTGCTCA TTCCTTTGTT TTCAGAACCC ACAAAAACAA CGATGCAAGG GCATACAGGA
    CGATTATTAA AATCTCTGTG TTTCACCAGT CTATCATTTC TGCTGCTGCA CATCATCTTT
    CAGATTACAA TAAACAGTCT TGAAGCTGGA AGAACTATTG AACCTGGTTT TAACTGTTCC
    ACATGGGAGA AGACATTACG GCAGATTGGT TTTGAAAGTG TGAAAGGAGC AGATGCCGGC
    AACGTGATCA GACTATTTGT ACCAGACATC GGGATGTTCA TTGCGAGCCT GACAATATGG
    CTCCTCTGCC GCAACATGGT TCAGAAGGCA ATAAATGAGG ATGCAGTGCA TTGCAACGCG
    CAGTTTGAAA ATGAGGAAAT GGCTGTAAGA GAAAAGTTAG AGCCAGACGA TGCTTTGATG
    TATGAAGAAG ACCTGGATGA TGACTGTGGT GAAGCAGAAT TCGAGGAAAC AATGAAATTA
    AAACTGCTGC GTAGAATTGC CTCCCTAGCA TCCAAACTGA GAGAGTTTAT TGGCAACATG
    ATAATCACCA CTGGAAAAGT TGTTGTTACA ATTTTACTTG GTTCAGCAGG TATGATGGTG
    CCATCCTTGA CCTCAGCTGT ATATTTCTTT GTATTTTTGG GCCTGTGCAC GTGGTGGTCC
    TGTTGCCAAG CATTTGATCC GCTGATATTC AGCTGTCTGT GTGTATTAAT GGCTATATTC
    AGTGCAGGGC ACTTGATTGG ACTTTATCTG TACCAGTTGC AGTTCTTCCA GGAGGTCGTT
    CCACCAAAAG ACTTCTATGC CAGGTTGTTT GGTGTCACTT CTCTTACGCA GACAAATTGC
    TCAAGCACTT GGATGATCAT CAAAAATCAA GAGTTACACT GGTATCACCA TGCCAATCCA
    GTTCTACTGC TGGTGATGTA CTACACCCTT GCAACTCTGA TCCGCCTTTG GCTGCAAGAG
    CCCATTCAGG TACCTGAGGA AGAGAAATCT GAGAGCAGGG AAGATGACAA AGAGATACCC
    TGCAGCCCAA TTCCAATGAC AGCAGAGAGA AGACGCAGTC TGTGGTACGC AAGCCACTAT
    ACAACTGATG AGAGAAAACT TCTGTCAATG ACCCAAGATG AATACAAGCC ATCGGATGTT
    CTGCTGGTAA CAGTGAACGG GAATCCTGCT GACTATCACA CCATCCACCC CTCACTGCCA
    TTAGAGAATG GTCCAGCCAA AGCAGACATG TACTCAACAC CACAGTACAA ATGGGAGCCC
    TCGGATAACA TGTCAGAAAA GGAAGAGGAG GAAGTAGAGG AGGAAGAAGA AGTTCCTCAG
    GAGGAAAAAG AAAATGTTAA ATTGCATGCC CTGGTCTCTG TGTTCCAATT TATCATGAAG
    CAAAGTTACA TTTGTGCTTT GATAGCTATG ATGGCATGGA GTATCACGTA TCACAGCTGG
    TTGACTTTTG TGCTCTTGAT CTGGTCTTGC ACGCTGTGGA TGATTCGAGA CCGAAGGAAA
    TATGCTATGA TCAGTTCACC GTTCATGGTT TTCTATGGAA ATTTACTGCT AATATTACAA
    TATATATGGA GTATTGAGCT CAAGAATGAT GAGCTTCCTC AAGTATCAGG TTTTTTAGAA
    AGAAAGGAAC CTGGAGAGTT GGCATCAAAG ATTCTTTTCA CGATTACTTT CTGGCTACTG
    CTTAGGCAAC ACCTCACTGA ACAGAAAGCA CTTCAGTTAA AAGAAGCTAC TTTATCTGAG
    GTCAAAGTTG GAAATGAAGA AGATGAAGAA AAAGAAGAGG GAGAGTTGCA AGAGGGAGAA
    GTGGCAGAAG AAGAGGAGGA GGAGGAGGAG GAACAGGAAG ATGATGATGA GGATGAGCAG
    GATATTATGA AAGTTCTGGG GAAGCTGGTG ATGGCTATGT TCATCAAGTA CTGGATTTAT
    GTCTGTGGAG GCATGTTCTT CTTCGTCAGC TTTGAGGGTA GAATTGTCAT GTACAAAATC
    ATCTACATGG TACTATTCCT GTTCTGTGTG GCCCTCTATC AGGTACACTA TGAATGGTGG
    AGAAAGATTC TGAAATACTT TTGGATGTCA GTTGTTGTTT ACACGATGTT GGTACTTATC
    TTCATCTACA CATACCAGTT TGAGTCATTC CCTGGGTTGT GGAAAAATAT GACAGGCCTG
    GATGAAAACA AACTAGCAGA CCTTGGTTTA AAAAGATTTT CTGTGGCTGA GCTATTCACT
    CGCATCTTTA TACCCACCTC CTTCCTACTG GCGTGTATCT TGCACCTTCA CTATTTCCAT
    GACCGTTTCC TCCAGCTCAC TGATTTAAAA GCTGTAACCA GCAAACAGGA CAACACTATT
    TACAGACTGG TGCATCAAGA TGGAAGCCTG CCTGACATAA CTATGATGAA TTTAACAGCC
    AGCAGTGAAA AGGAAGAGGA TAAAATGCTG AAAGAGGCTG GTGAGAAAAG AATGGAAGAG
    CCAGATGGGG AGGGAGGGAA AGGGAGGGCT GAAAAAGGAA AAGAAGAAGA AGAGGAAGAT
    GAGGAGAAGG AAGATGATGA TGTAGACGAT GAGTCAGAGG AAGAAGAAAC TACAGACCTA
    AGAAACAAGT GGCATCTGGT GATTGACCGC CTTACAGTGC TGTTTTTGAA ATTCCTGGAG
    TACTTTCATA AGATGCAAGT CTTTGTCTGG TGGCTTTTGG AGCTGCATAT CATCAAAATC
    GTCTCCTCTT ACATCATCTG GGTCACAGTA AAAGAGGTGT CTCTGTTTAA CTTTGTGTTT
    TTAATTGCTT GGGCTCTTGC TTTGCCATAT GCTCAGTTTC GCCCATTGGC ATCAAGTATC
    TGCACTGTTT GGACATGTGT GATTATTGTC TGCAAAATGT TGTACCAGCT CACATCTATT
    GATCCCAGCA CTTTTTCCAG TAACTGTACA TTGCCCAGAG AAAATGAAAC AAAGGTTGAT
    TTAGAAGAGC TCAAAACATC AGTCCTCTAC AGTGGTCCGG TTGATCCTGC AGAATGGGTT
    GGATTGAGGA AGTCTTATCC CCTACTTTTA TACTTAAGGA ATAACTTACT GATGCTGGCT
    ATTCTGGCTT TTGAAGTTAC AATTTACCGT CATCAAGAGT ACTACAGATG TCGAAATAAC
    CTTACTGCAC CTGTAACTAA AACTATTTTC CATGATATTA CAAGAGCACA TCTGGATGAT
    GGACTGGTAA ACTGTGTCAA GTATTTCATA AACTACTTCT TCTACAAGTT TGGTTTAGAG
    ACCTGTTTTC TTTTATCAGT GAATGTAATT GGTCAACGGA TGGATTTTTA TGCCATGATT
    CATGCCTTCT GGTTGATTGC TGTATTGTAT CGACGTAGAA GAAAAGCTAT TGCAGAGATC
    TGGCCAAAGT ATTGCTGTTT TCTGGCATGC ATCATTACAT TCCAGTATTT CCTTTGTATT
    GGCATTCCAC CTGCTCCTTG TAAAGACTAT CCATGGAGAA GTGGTAATGC CAACTTCAAC
    TCAAATATCA TAAAGTGGTT ATACTTTCCA GACTTCATTG TGAGACCAAA CCCTGTCTTC
    CTTGTCTATG ATTTCATGTT GCTGCTGTGT GCATCCTTAC AAAGGCAAAC GTTTGAGGAT
    GAAAATAAAG CTGCTGTGCG AATCATGGCT GGAGACAACG TGGAAATTTG TATGAATCTT
    GAAGCAGCAT CTTTTAGCCA GCATAATCCT GTTCCAGACT TCATTCACTG CCGGTCCTAC
    TTAGATATGT ATAAGGTGAT AATCTTTAGT TACCTCTTCT GGTTTGTGCT TACTATAATC
    TTCATAACGG GAACAACGAG GATCAGCATA TTCTGTATGG GCTACTTGGT TGCCTGCTTT
    TATTTCCTTC TCTTTGGTGG AGATCTGCTG CTGAAACCCA TCAGGAGCAT CCTGCGCTAC
    TGGGACTGGC TGATTGCATA CAATGTTTTT GTGATCACCA TGAAGAACAT CCTCTCGATA
    GGAGCATGTG GCTACATTGA GTCTTTAATT CAAAATAGTT GCTGGTTGAT TCAGGCATTT
    AGTCTGGCTT GCACAGTTAA AGGCTACCGT ATCCCAACCA ACAATGCAGA CTGTAAACTA
    CCTAGTGGAG AAGCAGGAAT CATCTGGGAC AGTATATGTT TTGCCTTTCT ACTGCTGCAA
    AGAAGAGTCT TCATGAGTTA CTACTTCCTC CACGTGGTTG CAGATATAAA AGCTTCTCAG
    ATCCTAGCAT CAAGGGGTGC TGAGCTTTTC CAGGCCACAA TTGTTAAGGC AGTAAAGGCA
    AGAATTGAAG AGGAAAAAAA ATCAATGGAT CAACTGAAAA GACAAATGGA TCGCATCAAA
    GCCAGGCAGC AAAAATATAA AAAGGGTAAA GAGAGGATGC TAAGCATGAC ACAGGATTCC
    ACAGAGGGGC CTGAAATTCG GAAGGTTTCG GAAGAAGATG ATGAAGGAGA AGCAGACAAA
    GAGAAAGCCA AGGGCAAAAA AAAGCTGTGG TGGCGGCCTT GGGTTGATCA TGCTTCCATG
    GTCAGAAGTG GAAATTATTA TTTGTTTGAG ACGGACAGTG AAGAGGAAGA GGAAGAAGAG
    AAAAAAGAAG AGGAAGAGCC TCGAAAAAAA TCTGCATTTC AGTTTGTTTA TCAGGCCTGG
    ATTACTGATC CTAAAACTGC GCTACGACAG AGGCGCAAGG AAAAGAAAAG CTTTCAAAAA
    GAGGAGAAGC GAAGGCGTAA AGTATATGGG GATGGAAGTA CTGATGCAGA CTGCGAAGAC
    AGTGAAGAAG AACCTGTCAA GAAGAAGTCT GATGGCCCAG ATAACATCAT CAAGAGGATA
    TTTAATATTT TGAAGTTCAC TTGGGTCCTT TTCCTGGCAA CACTAGACAG TTTTACAGCT
    TGGCTTAATT CCATCTCAAG AGAACACATC GATATCTCTA CTGTGCTGAG AATCGAACGT
    TGTATGCTGA CCAGGGAGAT TAAGAAGGGA AACGTTCCTA CCCGGGAGAG CATCCATATG
    TATTACCAGA ACCATATGAT GAAACTTTCT AAGGAGTCAG GACTGGACTC TATTGATAAA
    AATCCTGGGC AAGCTTCTGG TTTGCAAACA TCTGAAAGAA TGGATAGTTT AGATTCAGCA
    GCTTCTCGAG ACAGTATCTC CAGCGAGCCT ACACAGGTCA CCATGCTCTA TTCTCGTCAG
    GGGACGACAG AAACCATTGA GGAGGTAGAA GGAGAGCATG AAGAGGAAGG AGCTCATTCT
    GCTTCAAGGC AGGATGAAGA AGAGGTGGAA GATTATAGCC TGGGTTCAGA AGGAGCTGCA
    TATACTCCCG ACACCGATGT ACCATTGTAC TCCACTGTGG ACAGCAATGC AGAAGCACCA
    CCTTCCTATA GCAAAGCTGT CAGCTTTGAA CACCTTCCTT TTGGCTCCCC AGATGACTCA
    GCTGGAAAGA GTCTCATGAT GGTGAGCCCA GATGACAGCC GGACGGACAG ACTTAATGAT
    GCAATTCTCC CTCCATTGAC GCACGAGCTA ACGGCTAGTG AGCTGCTTCT GAACAAGATG
    TTTCATGATG ATGAGCTAGA GGAATCAGAG AAATTCTATG TTGGTCAGCC TCGAGTCTTG
    CTTCTTATTT ATGCGCTGTA CAATACTCTG GTGGCCCGGT CAGAAATGGT TTGCTATTTT
    GTCATCATCC TTAACCACAT GATCTCTGCC TCTATGATAA CGCTGGTGCT GCCCATCCTT
    ATCTTTCTTT GGGCCATGCT GTCTGTCCCT CGACCAAGCA AACGTTTCTG GATGACTGCC
    ATTGTCTACA CTGAGGTAGC CATTGTCATC AAGTACTTCT TCCAGTTTGG CTTCTTTCCT
    TGGAATAAAT ATGTGGATTA CACAAAAGAT AAACCTTATC ATCCACCCAA TATTATTGGG
    ATAGAGAAGA AAGAGGGCTA TGTGCACTAT GATCTTGTTC AGCTCCTTGC TTTATTTTTC
    CACAGATCCA TTTTAAAGTG CCATGGATTG TGGGATGAAG ATGAGAAAGG GGATAACTCC
    AGTAATAAAG GAGATACTGA TGATGAGCTT TCTCTGCCGG GAGGCAGGAG AGACTCTTCT
    GGGTCTCTGA AGTCGGTCAA TCTTGCAGCA TCAGTGGAGT CCATCCATGT GCACTTCCCT
    GAGCAGCAGA CAGCAATCAG GAGGAAGAGC TCCAGCAGTG CATCACAGCT TTCTCACAGA
    TCCAGCTTCT CCTCACACAG ATCTAAGAGA GGAAGCACCA GTACAAGGAA CAGCAGTCAG
    AAAGGGAGCA GTGTGCTAAG CATCAAGCAA AAATCTAGGA AAGAACTTCT TATGGAAAAG
    TTTCGAGAGC AAATGATCAA AGCCAAAGCC TTTACAATTA AAAAGACTCT CCAAGTGTAT
    GTGCCCATCA GACAATTTTT CTACAATCTT ATTCATCCAG ACTACAGTGC AGTGACTGAT
    GTATATGTGC TGATGTTCCT CGCAGATACA GTGGACTTCA TCATCATTGT CTTTGGATTT
    TGGGCTTTTG GGAAACACTC TGCAGCAGCA GATATTACCT CTTCATTATC AGAGGACCAG
    GTTCCAGAGG CATTTTTGGT GATGGTGCTC ATTCAGTTTG GTACCATGGT GGTAGACCGA
    GCGCTCTACC TCAAAAAGAC AGTCATGGGA AAGGTCATCT TCCAGGTCAT CCTTGTTTTT
    GGGATTCATT TCTGGATGTT CTTTATCTTG CCTGGAGTGA CAGAAAGAAA ATTTAGCCAG
    AACACCGTTG CCCAGCTCTG GTATTTTGTG AAATGTGTTT ATTTTGGCTT ATCAGCATAT
    CAGATACGCT GCGGATATCC AACTCGTGTT CTTGGCAACT TCCTCACAAA AAGTTATAAC
    TATGTGAACC TGTTCTTATT TCAAGGATTC CGCCTGGTTC CATTTTTGAC TGAGCTGAGA
    GCAGTAATGG ACTGGGTTTG GACAGATACC ACTCTCAGTC TCTCCAGCTG GATCTGTGTT
    GAAGATATCT ATGCTCATAT ATTCATCCTG AAGTGCTGGC GAGAGTCAGA AAAGAGATAT
    CCCCAACCAA GAGGCCAGAA GAAAAAGAAG GTTGTGAAGT ATGGAATGGG TGGCATGATT
    ATCGTCTTGC TGATTTGTAT TGTCTGGTTC CCACTGCTCT TCATGTCTTT AATCAAATCT
    GTTGCAGGCA TCACCAACAA GCCTCTAGAT GTATCAATCA CAATAACTCT AGGAGGTTAT
    CAGCCTATTT TTACAATGAG TGCTCAACAG AGTCAGCTCA AGGATTTGAA TCAGACTGGT
    TTCAGTGCAT TTCTGGGAAG CTATAGGGGT AACACTGCTG CTTTGCAGTT TTTAGAGGGC
    TATGGAAAAG AAGATATAAC CCTAGCAGAT CTAGAAGGGA ATTCAAACTC CTTATGGACC
    ATCAGCCCTC CCAGTAGAGA GAAAATGATA CAGGGACTGC TGGATTTTTC AGCTGAGTTC
    ACTGTAGTAC TTTCATGGAG CATTCAAAGA AATCTCACTC TTGGTGCCAA AGCAGAAATA
    GCATCAGATA AACTTACCTT TGGCCTACCA GAGAAAACCA GAAGAGATAT TGCTACAATG
    ATGTCTGGAA AGCCATTGGA AAAGGTAACA TTAGAAACAG TGTATCCATA CTACATCAAG
    GCTCCTAGTG ATTCTCTGGC AAAACCCATA AAGCAGCTAT TGACAGACTG CAGATGGGAA
    AATATCACAG TTTCCCTGGT CAAAAATGTG TCTGAAGAGG GAGTTCGTGA GTGGTGGGTT
    TTGAATCAGC TTGGAAAAAG ATACAAAACA AATGAAGAGA GCCTTGAACT CTTCATATTC
    AGTGATAAAG TCAGTCCTCC AAGCCTTGGA TTCTTGGCTG GATATGGCAT CATGGGACTA
    TACGCGTCAG TCGTCCTGGT GATAGGGAAG TTCGTCCGTG AGTTTTTCAG TGGGATTTCT
    CACTCCATCA TGTTTGAGGA GCTACCCAAT GTAGACCGAA TCTTGAAGTT GTGTACTGAC
    ATTTTCTTAG TACGAGAGAC TGGAGAACTG GAACTAGAAG AAGACCTGTA CGCCAAACTA
    ATCTTCCTGT ATCGCTCACC AGAGACTATG ATCAAGTGGA CTAGGGAAAA AACTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32432
    Clone ID Related Accession (Same CDS sequence) XM_015282541.1
    Accession Version XM_015282541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455796.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATCATCA AAAATCAAGA GTTACACTGG TATCACCATG CCAATCCAGT TCTACTGCTG 
    GTGATGTACT ACACCCTTGC AACTCTGATC CGCCTTTGGC TGCAAGAGCC CATTCAGGTA
    CCTGAGGAAG AGAAATCTGA GAGCAGGGAA GATGACAAAG AGATACCCTG CAGCCCAATT
    CCAATGACAG CAGAGAGAAG ACGCAGTCTG TGGTACGCAA GCCACTATAC AACTGATGAG
    AGAAAACTTC TGTCAATGAC CCAAGATGAA TACAAGCCAT CGGATGTTCT GCTGGTAACA
    GTGAACGGGA ATCCTGCTGA CTATCACACC ATCCACCCCT CACTGCCATT AGAGAATGGT
    CCAGCCAAAG CAGACATGTA CTCAACACCA CAGTACAAAT GGGAGCCCTC GGATAACATG
    TCAGAAAAGG AAGAGGAGGA AGTAGAGGAG GAAGAAGAAG TTCCTCAGGA GGAAAAAGAA
    AATGTTAAAT TGCATGCCCT GGTCTCTGTG TTCCAATTTA TCATGAAGCA AAGTTACATT
    TGTGCTTTGA TAGCTATGAT GGCATGGAGT ATCACGTATC ACAGCTGGTT GACTTTTGTG
    CTCTTGATCT GGTCTTGCAC GCTGTGGATG ATTCGAGACC GAAGGAAATA TGCTATGATC
    AGTTCACCGT TCATGGTTTT CTATGGAAAT TTACTGCTAA TATTACAATA TATATGGAGT
    ATTGAGCTCA AGAATGATGA GCTTCCTCAA GTATCAGGTT TTTTAGAAAG AAAGGAACCT
    GGAGAGTTGG CATCAAAGAT TCTTTTCACG ATTACTTTCT GGCTACTGCT TAGGCAACAC
    CTCACTGAAC AGAAAGCACT TCAGTTAAAA GAAGCTACTT TATCTGAGGT CAAAGTTGGA
    AGTGAAGAAG ATGAAGAAAA AGAAGAGGGA GAGTTGCAAG AGGGAGAAGT GGCAGAAGAA
    GAGGAGGAGG AGGAGGAGGA ACAGGAAGAT GATGATGAGG ATGAGCAGGA TATTATGAAA
    GTTCTGGGGA AGCTGGTGAT GGCTATGTTC ATCAAGTACT GGATTTATGT CTGTGGAGGC
    ATGTTCTTCT TCGTCAGCTT TGAGGGTAGA ATTGTCATGT ACAAAATCAT CTACATGGTA
    CTATTCCTGT TCTGTGTGGC CCTCTATCAG GTACACTATG AATGGTGGAG AAAGATTCTG
    AAATACTTTT GGATGTCAGT TGTTGTTTAC ACGATGTTGG TACTTATCTT CATCTACACA
    TACCAGTTTG AGTCATTCCC TGGGTTGTGG AAAAATATGA CAGGCCTGGA TGAAAACAAA
    CTAGCAGACC TTGGTTTAAA AAGATTTTCT GTGGCTGAGC TATTCACTCG CATCTTTATA
    CCCACCTCCT TCCTACTGGC GTGTATCTTG CACCTTCACT ATTTCCATGA CCGTTTCCTC
    CAGCTCACTG ATTTAAAAGC TGTAACCAGC AAACAGGACA ACACTATTTA CAGACTGGTG
    CATCAAGATG GAAGCCTGCC TGACATAACT ATGATGAATT TAACAGCCAG CAGTGAAAAG
    GAAGAGGATA AAATGCTGAA AGAGGCTGGT GAGAAAAGAA TGGAAGAGCC AGATGGGGAG
    GGAGGGAAAG GGAGGGCTGA AAAAGGAAAA GAAGAAGAAG AGGAAGATGA GGAGAAGGAA
    GATGATGATG TAGACGATGA GTCAGAGGAA GAAGAAACTA CAGACCTAAG AAACAAGTGG
    CATCTGGTGA TTGACCGCCT TACAGTGCTG TTTTTGAAAT TCCTGGAGTA CTTTCATAAG
    ATGCAAGTCT TTGTCTGGTG GCTTTTGGAG CTGCATATCA TCAAAATCGT CTCCTCTTAC
    ATCATCTGGG TCACAGTAAA AGAGGTGTCT CTGTTTAACT TTGTGTTTTT AATTGCTTGG
    GCTCTTGCTT TGCCATATGC TCAGTTTCGC CCATTGGCAT CAAGTATCTG CACTGTTTGG
    ACATGTGTGA TTATTGTCTG CAAAATGTTG TACCAGCTCA CATCTATTGA TCCCAGCACT
    TTTTCCAGTA ACTGTACATT GCCCAGAGAA AATGAAACAA AGGTTGATTT AGAAGAGCTC
    AAAACATCAG TCCTCTACAG TGGTCCGGTT GATCCTGCAG AATGGGTTGG ATTGAGGAAG
    TCTTATCCCC TACTTTTATA CTTAAGGAAT AACTTACTGA TGCTGGCTAT TCTGGCTTTT
    GAAGTTACAA TTTACCGTCA TCAAGAGTAC TACAGATGTC GAAATAACCT TACTGCACCT
    GTAACTAAAA CTATTTTCCA TGATATTACA AGAGCACATC TGGATGATGG ACTGGTAAAC
    TGTGTCAAGT ATTTCATAAA CTACTTCTTC TACAAGTTTG GTTTAGAGAC CTGTTTTCTT
    TTATCAGTGA ATGTAATTGG TCAACGGATG GATTTTTATG CCATGATTCA TGCCTTCTGG
    TTGATTGCTG TATTGTATCG ACGTAGAAGA AAAGCTATTG CAGAGATCTG GCCAAAGTAT
    TGCTGTTTTC TGGCATGCAT CATTACATTC CAGTATTTCC TTTGTATTGG CATTCCACCT
    GCTCCTTGTA AAGACTATCC ATGGAGAAGT GGTAATGCCA ACTTCAACTC AAATATCATA
    AAGTGGTTAT ACTTTCCAGA CTTCATTGTG AGACCAAACC CTGTCTTCCT TGTCTATGAT
    TTCATGTTGC TGCTGTGTGC ATCCTTACAA AGGCAAACGT TTGAGGATGA AAATAAAGCT
    GCTGTGCGAA TCATGGCTGG AGACAACGTG GAAATTTGTA TGAATCTTGA AGCAGCATCT
    TTTAGCCAGC ATAATCCTGT TCCAGACTTC ATTCACTGCC GGTCCTACTT AGATATGTAT
    AAGGTGATAA TCTTTAGTTA CCTCTTCTGG TTTGTGCTTA CTATAATCTT CATAACGGGA
    ACAACGAGGA TCAGCATATT CTGTATGGGC TACTTGGTTG CCTGCTTTTA TTTCCTTCTC
    TTTGGTGGAG ATCTGCTGCT GAAACCCATC AGGAGCATCC TGCGCTACTG GGACTGGCTG
    ATTGCATACA ATGTTTTTGT GATCACCATG AAGAACATCC TCTCGATAGG AGCATGTGGC
    TACATTGAGT CTTTAATTCA AAATAGTTGC TGGTTGATTC AGGCATTTAG TCTGGCTTGC
    ACAGTTAAAG GCTACCGTAT CCCAACCAAC AATGCAGACT GTAAACTACC TAGTGGAGAA
    GCAGGAATCA TCTGGGACAG TATATGTTTT GCCTTTCTAC TGCTGCAAAG AAGAGTCTTC
    ATGAGTTACT ACTTCCTCCA CGTGGTTGCA GATATAAAAG CTTCTCAGAT CCTAGCATCA
    AGGGGTGCTG AGCTTTTCCA GGCCACAATT GTTAAGGCAG TAAAGGCAAG AATTGAAGAG
    GAAAAAAAAT CAATGGATCA ACTGAAAAGA CAAATGGATC GCATCAAAGC CAGGCAGCAA
    AAATATAAAA AGGGTAAAGA GAGGATGCTA AGCATGACAC AGGATTCCAC AGAGGGGCCT
    GAAATTCGGA AGGTTTCGGA AGAAGATGAT GAAGGAGAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCTGTGGTG GCGGCCTTGG GTTGATCATG CTTCCATGGT CAGAAGTGGA
    AATTATTATT TGTTTGAGAC GGACAGTGAA GAGGAAGAGG AAGAAGAGAA AAAAGAAGAG
    GAAGAGCCTC GAAAAAAATC TGCATTTCAG TTTGTTTATC AGGCCTGGAT TACTGATCCT
    AAAACTGCGC TACGACAGAG GCGCAAGGAA AAGAAAAGCT TTCAAAAAGA GGAGAAGCGA
    AGGCGTAAAG TATATGGGGA TGGAAGTACT GATGCAGACT GCGAAGACAG TGAAGAAGAA
    CCTGTCAAGA AGAAGTCTGA TGGCCCAGAT AACATCATCA AGAGGATATT TAATATTTTG
    AAGTTCACTT GGGTCCTTTT CCTGGCAACA CTAGACAGTT TTACAGCTTG GCTTAATTCC
    ATCTCAAGAG AACACATCGA TATCTCTACT GTGCTGAGAA TCGAACGTTG TATGCTGACC
    AGGGAGATTA AGAAGGGAAA CGTTCCTACC CGGGAGAGCA TCCATATGTA TTACCAGAAC
    CATATGATGA AACTTTCTAA GGAGTCAGGA CTGGACTCTA TTGATAAAAA TCCTGGGCAA
    GCTTCTGGTT TGCAAACATC TGAAAGAATG GATAGTTTAG ATTCAGCAGC TTCTCGAGAC
    AGTATCTCCA GCTGCTACAC TGAAGCAACC ATGCTGTTCT CAAGGCAGTC AACCCTTGAT
    GATTTAGATG GACCAGACAC TGTTCCTAAA ACAAGTGAGC GCGCTCGACC TAGGCTTCGT
    AAAATGCAGA GCATGGATAT GTCCTCTTCA TCAGCTGACA GTGGCAGTAT AGTGTCAAGC
    GAGCCTACAC AGGTCACCAT GCTCTATTCT CGTCAGGGGA CGACAGAAAC CATTGAGGAG
    GTAGAAGGAG AGCATGAAGA GGAAGGAGCT CATTCTGCTT CAAGGCAGGA TGAAGAAGAG
    GTGGAAGATT ATAGCCTGGG TTCAGAAGGA GCTGCATATA CTCCCGACAC CGATGTACCA
    TTGTACTCCA CTGTGGACAG CAATGCAGAA GCACCACCTT CCTATAGCAA AGCTGTCAGC
    TTTGAACACC TTCCTTTTGG CTCCCCAGAT GACTCAGCTG GAAAGAGTCT CATGATGGTG
    AGCCCAGATG ACAGCCGGAC GGACAGACTT AATGATGCAA TTCTCCCTCC ATTGACGCAC
    GAGCTAACGG CTAGTGAGCT GCTTCTGAAC AAGATGTTTC ATGATGATGA GCTAGAGGAA
    TCAGAGAAAT TCTATGTTGG TCAGCCTCGA GTCTTGCTTC TTATTTATGC GCTGTACAAT
    ACTCTGGTGG CCCGGTCAGA AATGGTTTGC TATTTTGTCA TCATCCTTAA CCACATGATC
    TCTGCCTCTA TGATAACGCT GGTGCTGCCC ATCCTTATCT TTCTTTGGGC CATGCTGTCT
    GTCCCTCGAC CAAGCAAACG TTTCTGGATG ACTGCCATTG TCTACACTGA GGTAGCCATT
    GTCATCAAGT ACTTCTTCCA GTTTGGCTTC TTTCCTTGGA ATAAATATGT GGATTACACA
    AAAGATAAAC CTTATCATCC ACCCAATATT ATTGGGATAG AGAAGAAAGA GGGCTATGTG
    CACTATGATC TTGTTCAGCT CCTTGCTTTA TTTTTCCACA GATCCATTTT AAAGTGCCAT
    GGATTGTGGG ATGAAGATGA GAAAGGGGAT AACTCCAGTA ATAAAGGAGA TACTGATGAT
    GAGCTTTCTC TGCCGGGAGG CAGGAGAGAC TCTTCTGGGT CTCTGAAGTC GGTCAATCTT
    GCAGCATCAG TGGAGTCCAT CCATGTGCAC TTCCCTGAGC AGCAGACAGC AATCAGGAGG
    AAGAGCTCCA GCAGTGCATC ACAGCTTTCT CACAGATCCA GCTTCTCCTC ACACAGATCT
    AAGAGAGGAA GCACCAGTAC AAGGAACAGC AGTCAGAAAG GGAGCAGTGT GCTAAGCATC
    AAGCAAAAAT CTAGGAAAGA ACTTCTTATG GAAAAGTTTC GAGAGCAAAT GATCAAAGCC
    AAAGCCTTTA CAATTAAAAA GACTCTCCAA GTGTATGTGC CCATCAGACA ATTTTTCTAC
    AATCTTATTC ATCCAGACTA CAGTGCAGTG ACTGATGTAT ATGTGCTGAT GTTCCTCGCA
    GATACAGTGG ACTTCATCAT CATTGTCTTT GGATTTTGGG CTTTTGGGAA ACACTCTGCA
    GCAGCAGATA TTACCTCTTC ATTATCAGAG GACCAGGTTC CAGAGGCATT TTTGGTGATG
    GTGCTCATTC AGTTTGGTAC CATGGTGGTA GACCGAGCGC TCTACCTCAA AAAGACAGTC
    ATGGGAAAGG TCATCTTCCA GGTCATCCTT GTTTTTGGGA TTCATTTCTG GATGTTCTTT
    ATCTTGCCTG GAGTGACAGA AAGAAAATTT AGCCAGAACA CCGTTGCCCA GCTCTGGTAT
    TTTGTGAAAT GTGTTTATTT TGGCTTATCA GCATATCAGA TACGCTGCGG ATATCCAACT
    CGTGTTCTTG GCAACTTCCT CACAAAAAGT TATAACTATG TGAACCTGTT CTTATTTCAA
    GGATTCCGCC TGGTTCCATT TTTGACTGAG CTGAGAGCAG TAATGGACTG GGTTTGGACA
    GATACCACTC TCAGTCTCTC CAGCTGGATC TGTGTTGAAG ATATCTATGC TCATATATTC
    ATCCTGAAGT GCTGGCGAGA GTCAGAAAAG AGATATCCCC AACCAAGAGG CCAGAAGAAA
    AAGAAGGTTG TGAAGTATGG AATGGGTGGC ATGATTATCG TCTTGCTGAT TTGTATTGTC
    TGGTTCCCAC TGCTCTTCAT GTCTTTAATC AAATCTGTTG CAGGCATCAC CAACAAGCCT
    CTAGATGTAT CAATCACAAT AACTCTAGGA GGTTATCAGC CTATTTTTAC AATGAGTGCT
    CAACAGAGTC AGCTCAAGGA TTTGAATCAG ACTGGTTTCA GTGCATTTCT GGGAAGCTAT
    AGGGGTAACA CTGCTGCTTT GCAGTTTTTA GAGGGCTATG GAAAAGAAGA TATAACCCTA
    GCAGATCTAG AAGGGAATTC AAACTCCTTA TGGACCATCA GCCCTCCCAG TAGAGAGAAA
    ATGATACAGG GACTGCTGGA TTTTTCAGCT GAGTTCACTG TAGTACTTTC ATGGAGCATT
    CAAAGAAATC TCACTCTTGG TGCCAAAGCA GAAATAGCAT CAGATAAACT TACCTTTGGC
    CTACCAGAGA AAACCAGAAG AGATATTGCT ACAATGATGT CTGGAAAGCC ATTGGAAAAG
    GTAACATTAG AAACAGTGTA TCCATACTAC ATCAAGGCTC CTAGTGATTC TCTGGCAAAA
    CCCATAAAGC AGCTATTGAC AGACTGCAGA TGGGAAAATA TCACAGTTTC CCTGGTCAAA
    AATGTGTCTG AAGAGGGAGT TCGTGAGTGG TGGGTTTTGA ATCAGCTTGG AAAAAGATAC
    AAAACAAATG AAGAGAGCCT TGAACTCTTC ATATTCAGTG ATAAAGTCAG TCCTCCAAGC
    CTTGGATTCT TGGCTGGATA TGGCATCATG GGACTATACG CGTCAGTCGT CCTGGTGATA
    GGGAAGTTCG TCCGTGAGTT TTTCAGTGGG ATTTCTCACT CCATCATGTT TGAGGAGCTA
    CCCAATGTAG ACCGAATCTT GAAGTTGTGT ACTGACATTT TCTTAGTACG AGAGACTGGA
    GAACTGGAAC TAGAAGAAGA CCTGTACGCC AAACTAATCT TCCTGTATCG CTCACCAGAG
    ACTATGATCA AGTGGACTAG GGAAAAAACT AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138027.1
    CDS52..7467
    Translation

    Target ORF information:

    RefSeq Version XM_015282541.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus family with sequence similarity 38, member B (FAM38B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGATCATCA AAAATCAAGA GTTACACTGG TATCACCATG CCAATCCAGT TCTACTGCTG 
    GTGATGTACT ACACCCTTGC AACTCTGATC CGCCTTTGGC TGCAAGAGCC CATTCAGGTA
    CCTGAGGAAG AGAAATCTGA GAGCAGGGAA GATGACAAAG AGATACCCTG CAGCCCAATT
    CCAATGACAG CAGAGAGAAG ACGCAGTCTG TGGTACGCAA GCCACTATAC AACTGATGAG
    AGAAAACTTC TGTCAATGAC CCAAGATGAA TACAAGCCAT CGGATGTTCT GCTGGTAACA
    GTGAACGGGA ATCCTGCTGA CTATCACACC ATCCACCCCT CACTGCCATT AGAGAATGGT
    CCAGCCAAAG CAGACATGTA CTCAACACCA CAGTACAAAT GGGAGCCCTC GGATAACATG
    TCAGAAAAGG AAGAGGAGGA AGTAGAGGAG GAAGAAGAAG TTCCTCAGGA GGAAAAAGAA
    AATGTTAAAT TGCATGCCCT GGTCTCTGTG TTCCAATTTA TCATGAAGCA AAGTTACATT
    TGTGCTTTGA TAGCTATGAT GGCATGGAGT ATCACGTATC ACAGCTGGTT GACTTTTGTG
    CTCTTGATCT GGTCTTGCAC GCTGTGGATG ATTCGAGACC GAAGGAAATA TGCTATGATC
    AGTTCACCGT TCATGGTTTT CTATGGAAAT TTACTGCTAA TATTACAATA TATATGGAGT
    ATTGAGCTCA AGAATGATGA GCTTCCTCAA GTATCAGGTT TTTTAGAAAG AAAGGAACCT
    GGAGAGTTGG CATCAAAGAT TCTTTTCACG ATTACTTTCT GGCTACTGCT TAGGCAACAC
    CTCACTGAAC AGAAAGCACT TCAGTTAAAA GAAGCTACTT TATCTGAGGT CAAAGTTGGA
    AGTGAAGAAG ATGAAGAAAA AGAAGAGGGA GAGTTGCAAG AGGGAGAAGT GGCAGAAGAA
    GAGGAGGAGG AGGAGGAGGA ACAGGAAGAT GATGATGAGG ATGAGCAGGA TATTATGAAA
    GTTCTGGGGA AGCTGGTGAT GGCTATGTTC ATCAAGTACT GGATTTATGT CTGTGGAGGC
    ATGTTCTTCT TCGTCAGCTT TGAGGGTAGA ATTGTCATGT ACAAAATCAT CTACATGGTA
    CTATTCCTGT TCTGTGTGGC CCTCTATCAG GTACACTATG AATGGTGGAG AAAGATTCTG
    AAATACTTTT GGATGTCAGT TGTTGTTTAC ACGATGTTGG TACTTATCTT CATCTACACA
    TACCAGTTTG AGTCATTCCC TGGGTTGTGG AAAAATATGA CAGGCCTGGA TGAAAACAAA
    CTAGCAGACC TTGGTTTAAA AAGATTTTCT GTGGCTGAGC TATTCACTCG CATCTTTATA
    CCCACCTCCT TCCTACTGGC GTGTATCTTG CACCTTCACT ATTTCCATGA CCGTTTCCTC
    CAGCTCACTG ATTTAAAAGC TGTAACCAGC AAACAGGACA ACACTATTTA CAGACTGGTG
    CATCAAGATG GAAGCCTGCC TGACATAACT ATGATGAATT TAACAGCCAG CAGTGAAAAG
    GAAGAGGATA AAATGCTGAA AGAGGCTGGT GAGAAAAGAA TGGAAGAGCC AGATGGGGAG
    GGAGGGAAAG GGAGGGCTGA AAAAGGAAAA GAAGAAGAAG AGGAAGATGA GGAGAAGGAA
    GATGATGATG TAGACGATGA GTCAGAGGAA GAAGAAACTA CAGACCTAAG AAACAAGTGG
    CATCTGGTGA TTGACCGCCT TACAGTGCTG TTTTTGAAAT TCCTGGAGTA CTTTCATAAG
    ATGCAAGTCT TTGTCTGGTG GCTTTTGGAG CTGCATATCA TCAAAATCGT CTCCTCTTAC
    ATCATCTGGG TCACAGTAAA AGAGGTGTCT CTGTTTAACT TTGTGTTTTT AATTGCTTGG
    GCTCTTGCTT TGCCATATGC TCAGTTTCGC CCATTGGCAT CAAGTATCTG CACTGTTTGG
    ACATGTGTGA TTATTGTCTG CAAAATGTTG TACCAGCTCA CATCTATTGA TCCCAGCACT
    TTTTCCAGTA ACTGTACATT GCCCAGAGAA AATGAAACAA AGGTTGATTT AGAAGAGCTC
    AAAACATCAG TCCTCTACAG TGGTCCGGTT GATCCTGCAG AATGGGTTGG ATTGAGGAAG
    TCTTATCCCC TACTTTTATA CTTAAGGAAT AACTTACTGA TGCTGGCTAT TCTGGCTTTT
    GAAGTTACAA TTTACCGTCA TCAAGAGTAC TACAGATGTC GAAATAACCT TACTGCACCT
    GTAACTAAAA CTATTTTCCA TGATATTACA AGAGCACATC TGGATGATGG ACTGGTAAAC
    TGTGTCAAGT ATTTCATAAA CTACTTCTTC TACAAGTTTG GTTTAGAGAC CTGTTTTCTT
    TTATCAGTGA ATGTAATTGG TCAACGGATG GATTTTTATG CCATGATTCA TGCCTTCTGG
    TTGATTGCTG TATTGTATCG ACGTAGAAGA AAAGCTATTG CAGAGATCTG GCCAAAGTAT
    TGCTGTTTTC TGGCATGCAT CATTACATTC CAGTATTTCC TTTGTATTGG CATTCCACCT
    GCTCCTTGTA AAGACTATCC ATGGAGAAGT GGTAATGCCA ACTTCAACTC AAATATCATA
    AAGTGGTTAT ACTTTCCAGA CTTCATTGTG AGACCAAACC CTGTCTTCCT TGTCTATGAT
    TTCATGTTGC TGCTGTGTGC ATCCTTACAA AGGCAAACGT TTGAGGATGA AAATAAAGCT
    GCTGTGCGAA TCATGGCTGG AGACAACGTG GAAATTTGTA TGAATCTTGA AGCAGCATCT
    TTTAGCCAGC ATAATCCTGT TCCAGACTTC ATTCACTGCC GGTCCTACTT AGATATGTAT
    AAGGTGATAA TCTTTAGTTA CCTCTTCTGG TTTGTGCTTA CTATAATCTT CATAACGGGA
    ACAACGAGGA TCAGCATATT CTGTATGGGC TACTTGGTTG CCTGCTTTTA TTTCCTTCTC
    TTTGGTGGAG ATCTGCTGCT GAAACCCATC AGGAGCATCC TGCGCTACTG GGACTGGCTG
    ATTGCATACA ATGTTTTTGT GATCACCATG AAGAACATCC TCTCGATAGG AGCATGTGGC
    TACATTGAGT CTTTAATTCA AAATAGTTGC TGGTTGATTC AGGCATTTAG TCTGGCTTGC
    ACAGTTAAAG GCTACCGTAT CCCAACCAAC AATGCAGACT GTAAACTACC TAGTGGAGAA
    GCAGGAATCA TCTGGGACAG TATATGTTTT GCCTTTCTAC TGCTGCAAAG AAGAGTCTTC
    ATGAGTTACT ACTTCCTCCA CGTGGTTGCA GATATAAAAG CTTCTCAGAT CCTAGCATCA
    AGGGGTGCTG AGCTTTTCCA GGCCACAATT GTTAAGGCAG TAAAGGCAAG AATTGAAGAG
    GAAAAAAAAT CAATGGATCA ACTGAAAAGA CAAATGGATC GCATCAAAGC CAGGCAGCAA
    AAATATAAAA AGGGTAAAGA GAGGATGCTA AGCATGACAC AGGATTCCAC AGAGGGGCCT
    GAAATTCGGA AGGTTTCGGA AGAAGATGAT GAAGGAGAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCTGTGGTG GCGGCCTTGG GTTGATCATG CTTCCATGGT CAGAAGTGGA
    AATTATTATT TGTTTGAGAC GGACAGTGAA GAGGAAGAGG AAGAAGAGAA AAAAGAAGAG
    GAAGAGCCTC GAAAAAAATC TGCATTTCAG TTTGTTTATC AGGCCTGGAT TACTGATCCT
    AAAACTGCGC TACGACAGAG GCGCAAGGAA AAGAAAAGCT TTCAAAAAGA GGAGAAGCGA
    AGGCGTAAAG TATATGGGGA TGGAAGTACT GATGCAGACT GCGAAGACAG TGAAGAAGAA
    CCTGTCAAGA AGAAGTCTGA TGGCCCAGAT AACATCATCA AGAGGATATT TAATATTTTG
    AAGTTCACTT GGGTCCTTTT CCTGGCAACA CTAGACAGTT TTACAGCTTG GCTTAATTCC
    ATCTCAAGAG AACACATCGA TATCTCTACT GTGCTGAGAA TCGAACGTTG TATGCTGACC
    AGGGAGATTA AGAAGGGAAA CGTTCCTACC CGGGAGAGCA TCCATATGTA TTACCAGAAC
    CATATGATGA AACTTTCTAA GGAGTCAGGA CTGGACTCTA TTGATAAAAA TCCTGGGCAA
    GCTTCTGGTT TGCAAACATC TGAAAGAATG GATAGTTTAG ATTCAGCAGC TTCTCGAGAC
    AGTATCTCCA GCTGCTACAC TGAAGCAACC ATGCTGTTCT CAAGGCAGTC AACCCTTGAT
    GATTTAGATG GACCAGACAC TGTTCCTAAA ACAAGTGAGC GCGCTCGACC TAGGCTTCGT
    AAAATGCAGA GCATGGATAT GTCCTCTTCA TCAGCTGACA GTGGCAGTAT AGTGTCAAGC
    GAGCCTACAC AGGTCACCAT GCTCTATTCT CGTCAGGGGA CGACAGAAAC CATTGAGGAG
    GTAGAAGGAG AGCATGAAGA GGAAGGAGCT CATTCTGCTT CAAGGCAGGA TGAAGAAGAG
    GTGGAAGATT ATAGCCTGGG TTCAGAAGGA GCTGCATATA CTCCCGACAC CGATGTACCA
    TTGTACTCCA CTGTGGACAG CAATGCAGAA GCACCACCTT CCTATAGCAA AGCTGTCAGC
    TTTGAACACC TTCCTTTTGG CTCCCCAGAT GACTCAGCTG GAAAGAGTCT CATGATGGTG
    AGCCCAGATG ACAGCCGGAC GGACAGACTT AATGATGCAA TTCTCCCTCC ATTGACGCAC
    GAGCTAACGG CTAGTGAGCT GCTTCTGAAC AAGATGTTTC ATGATGATGA GCTAGAGGAA
    TCAGAGAAAT TCTATGTTGG TCAGCCTCGA GTCTTGCTTC TTATTTATGC GCTGTACAAT
    ACTCTGGTGG CCCGGTCAGA AATGGTTTGC TATTTTGTCA TCATCCTTAA CCACATGATC
    TCTGCCTCTA TGATAACGCT GGTGCTGCCC ATCCTTATCT TTCTTTGGGC CATGCTGTCT
    GTCCCTCGAC CAAGCAAACG TTTCTGGATG ACTGCCATTG TCTACACTGA GGTAGCCATT
    GTCATCAAGT ACTTCTTCCA GTTTGGCTTC TTTCCTTGGA ATAAATATGT GGATTACACA
    AAAGATAAAC CTTATCATCC ACCCAATATT ATTGGGATAG AGAAGAAAGA GGGCTATGTG
    CACTATGATC TTGTTCAGCT CCTTGCTTTA TTTTTCCACA GATCCATTTT AAAGTGCCAT
    GGATTGTGGG ATGAAGATGA GAAAGGGGAT AACTCCAGTA ATAAAGGAGA TACTGATGAT
    GAGCTTTCTC TGCCGGGAGG CAGGAGAGAC TCTTCTGGGT CTCTGAAGTC GGTCAATCTT
    GCAGCATCAG TGGAGTCCAT CCATGTGCAC TTCCCTGAGC AGCAGACAGC AATCAGGAGG
    AAGAGCTCCA GCAGTGCATC ACAGCTTTCT CACAGATCCA GCTTCTCCTC ACACAGATCT
    AAGAGAGGAA GCACCAGTAC AAGGAACAGC AGTCAGAAAG GGAGCAGTGT GCTAAGCATC
    AAGCAAAAAT CTAGGAAAGA ACTTCTTATG GAAAAGTTTC GAGAGCAAAT GATCAAAGCC
    AAAGCCTTTA CAATTAAAAA GACTCTCCAA GTGTATGTGC CCATCAGACA ATTTTTCTAC
    AATCTTATTC ATCCAGACTA CAGTGCAGTG ACTGATGTAT ATGTGCTGAT GTTCCTCGCA
    GATACAGTGG ACTTCATCAT CATTGTCTTT GGATTTTGGG CTTTTGGGAA ACACTCTGCA
    GCAGCAGATA TTACCTCTTC ATTATCAGAG GACCAGGTTC CAGAGGCATT TTTGGTGATG
    GTGCTCATTC AGTTTGGTAC CATGGTGGTA GACCGAGCGC TCTACCTCAA AAAGACAGTC
    ATGGGAAAGG TCATCTTCCA GGTCATCCTT GTTTTTGGGA TTCATTTCTG GATGTTCTTT
    ATCTTGCCTG GAGTGACAGA AAGAAAATTT AGCCAGAACA CCGTTGCCCA GCTCTGGTAT
    TTTGTGAAAT GTGTTTATTT TGGCTTATCA GCATATCAGA TACGCTGCGG ATATCCAACT
    CGTGTTCTTG GCAACTTCCT CACAAAAAGT TATAACTATG TGAACCTGTT CTTATTTCAA
    GGATTCCGCC TGGTTCCATT TTTGACTGAG CTGAGAGCAG TAATGGACTG GGTTTGGACA
    GATACCACTC TCAGTCTCTC CAGCTGGATC TGTGTTGAAG ATATCTATGC TCATATATTC
    ATCCTGAAGT GCTGGCGAGA GTCAGAAAAG AGATATCCCC AACCAAGAGG CCAGAAGAAA
    AAGAAGGTTG TGAAGTATGG AATGGGTGGC ATGATTATCG TCTTGCTGAT TTGTATTGTC
    TGGTTCCCAC TGCTCTTCAT GTCTTTAATC AAATCTGTTG CAGGCATCAC CAACAAGCCT
    CTAGATGTAT CAATCACAAT AACTCTAGGA GGTTATCAGC CTATTTTTAC AATGAGTGCT
    CAACAGAGTC AGCTCAAGGA TTTGAATCAG ACTGGTTTCA GTGCATTTCT GGGAAGCTAT
    AGGGGTAACA CTGCTGCTTT GCAGTTTTTA GAGGGCTATG GAAAAGAAGA TATAACCCTA
    GCAGATCTAG AAGGGAATTC AAACTCCTTA TGGACCATCA GCCCTCCCAG TAGAGAGAAA
    ATGATACAGG GACTGCTGGA TTTTTCAGCT GAGTTCACTG TAGTACTTTC ATGGAGCATT
    CAAAGAAATC TCACTCTTGG TGCCAAAGCA GAAATAGCAT CAGATAAACT TACCTTTGGC
    CTACCAGAGA AAACCAGAAG AGATATTGCT ACAATGATGT CTGGAAAGCC ATTGGAAAAG
    GTAACATTAG AAACAGTGTA TCCATACTAC ATCAAGGCTC CTAGTGATTC TCTGGCAAAA
    CCCATAAAGC AGCTATTGAC AGACTGCAGA TGGGAAAATA TCACAGTTTC CCTGGTCAAA
    AATGTGTCTG AAGAGGGAGT TCGTGAGTGG TGGGTTTTGA ATCAGCTTGG AAAAAGATAC
    AAAACAAATG AAGAGAGCCT TGAACTCTTC ATATTCAGTG ATAAAGTCAG TCCTCCAAGC
    CTTGGATTCT TGGCTGGATA TGGCATCATG GGACTATACG CGTCAGTCGT CCTGGTGATA
    GGGAAGTTCG TCCGTGAGTT TTTCAGTGGG ATTTCTCACT CCATCATGTT TGAGGAGCTA
    CCCAATGTAG ACCGAATCTT GAAGTTGTGT ACTGACATTT TCTTAGTACG AGAGACTGGA
    GAACTGGAAC TAGAAGAAGA CCTGTACGCC AAACTAATCT TCCTGTATCG CTCACCAGAG
    ACTATGATCA AGTGGACTAG GGAAAAAACT AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32433
    Clone ID Related Accession (Same CDS sequence) XM_015282542.1
    Accession Version XM_015282542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455796.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGATCATCA AAAATCAAGA GTTACACTGG TATCACCATG CCAATCCAGT TCTACTGCTG 
    GTGATGTACT ACACCCTTGC AACTCTGATC CGCCTTTGGC TGCAAGAGCC CATTCAGGTA
    CCTGAGGAAG AGAAATCTGA GAGCAGGGAA GATGACAAAG AGATACCCTG CAGCCCAATT
    CCAATGACAG CAGAGAGAAG ACGCAGTCTG TGGTACGCAA GCCACTATAC AACTGATGAG
    AGAAAAGTTC TGCTGGTAAC AGTGAACGGG AATCCTGCTG ACTATCACAC CATCCACCCC
    TCACTGCCAT TAGAGAATGG TCCAGCCAAA GCAGACATGT ACTCAACACC ACAGTACAAA
    TGGGAGCCCT CGGATAACAT GTCAGAAAAG GAAGAGGAGG AAGTAGAGGA GGAAGAAGAA
    GTTCCTCAGG AGGAAAAAGA AAATGTTAAA TTGCATGCCC TGGTCTCTGT GTTCCAATTT
    ATCATGAAGC AAAGTTACAT TTGTGCTTTG ATAGCTATGA TGGCATGGAG TATCACGTAT
    CACAGCTGGT TGACTTTTGT GCTCTTGATC TGGTCTTGCA CGCTGTGGAT GATTCGAGAC
    CGAAGGAAAT ATGCTATGAT CAGTTCACCG TTCATGGTTT TCTATGGAAA TTTACTGCTA
    ATATTACAAT ATATATGGAG TATTGAGCTC AAGAATGATG AGCTTCCTCA AGTATCAGGT
    TTTTTAGAAA GAAAGGAACC TGGAGAGTTG GCATCAAAGA TTCTTTTCAC GATTACTTTC
    TGGCTACTGC TTAGGCAACA CCTCACTGAA CAGAAAGCAC TTCAGTTAAA AGAAGCTACT
    TTATCTGAGG TCAAAGTTGG AAGTGAAGAA GATGAAGAAA AAGAAGAGGG AGAGTTGCAA
    GAGGGAGAAG TGGCAGAAGA AGAGGAGGAG GAGGAGGAGG AACAGGAAGA TGATGATGAG
    GATGAGCAGG ATATTATGAA AGTTCTGGGG AAGCTGGTGA TGGCTATGTT CATCAAGTAC
    TGGATTTATG TCTGTGGAGG CATGTTCTTC TTCGTCAGCT TTGAGGGTAG AATTGTCATG
    TACAAAATCA TCTACATGGT ACTATTCCTG TTCTGTGTGG CCCTCTATCA GGTACACTAT
    GAATGGTGGA GAAAGATTCT GAAATACTTT TGGATGTCAG TTGTTGTTTA CACGATGTTG
    GTACTTATCT TCATCTACAC ATACCAGTTT GAGTCATTCC CTGGGTTGTG GAAAAATATG
    ACAGGCCTGG ATGAAAACAA ACTAGCAGAC CTTGGTTTAA AAAGATTTTC TGTGGCTGAG
    CTATTCACTC GCATCTTTAT ACCCACCTCC TTCCTACTGG CGTGTATCTT GCACCTTCAC
    TATTTCCATG ACCGTTTCCT CCAGCTCACT GATTTAAAAG CTGTAACCAG CAAACAGGAC
    AACACTATTT ACAGACTGGT GCATCAAGAT GGAAGCCTGC CTGACATAAC TATGATGAAT
    TTAACAGCCA GCAGTGAAAA GGAAGAGGAT AAAATGCTGA AAGAGGCTGG TGAGAAAAGA
    ATGGAAGAGC CAGATGGGGA GGGAGGGAAA GGGAGGGCTG AAAAAGGAAA AGAAGAAGAA
    GAGGAAGATG AGGAGAAGGA AGATGATGAT GTAGACGATG AGTCAGAGGA AGAAGAAACT
    ACAGACCTAA GAAACAAGTG GCATCTGGTG ATTGACCGCC TTACAGTGCT GTTTTTGAAA
    TTCCTGGAGT ACTTTCATAA GATGCAAGTC TTTGTCTGGT GGCTTTTGGA GCTGCATATC
    ATCAAAATCG TCTCCTCTTA CATCATCTGG GTCACAGTAA AAGAGGTGTC TCTGTTTAAC
    TTTGTGTTTT TAATTGCTTG GGCTCTTGCT TTGCCATATG CTCAGTTTCG CCCATTGGCA
    TCAAGTATCT GCACTGTTTG GACATGTGTG ATTATTGTCT GCAAAATGTT GTACCAGCTC
    ACATCTATTG ATCCCAGCAC TTTTTCCAGT AACTGTACAT TGCCCAGAGA AAATGAAACA
    AAGGTTGATT TAGAAGAGCT CAAAACATCA GTCCTCTACA GTGGTCCGGT TGATCCTGCA
    GAATGGGTTG GATTGAGGAA GTCTTATCCC CTACTTTTAT ACTTAAGGAA TAACTTACTG
    ATGCTGGCTA TTCTGGCTTT TGAAGTTACA ATTTACCGTC ATCAAGAGTA CTACAGATGT
    CGAAATAACC TTACTGCACC TGTAACTAAA ACTATTTTCC ATGATATTAC AAGAGCACAT
    CTGGATGATG GACTGGTAAA CTGTGTCAAG TATTTCATAA ACTACTTCTT CTACAAGTTT
    GGTTTAGAGA CCTGTTTTCT TTTATCAGTG AATGTAATTG GTCAACGGAT GGATTTTTAT
    GCCATGATTC ATGCCTTCTG GTTGATTGCT GTATTGTATC GACGTAGAAG AAAAGCTATT
    GCAGAGATCT GGCCAAAGTA TTGCTGTTTT CTGGCATGCA TCATTACATT CCAGTATTTC
    CTTTGTATTG GCATTCCACC TGCTCCTTGT AAAGACTATC CATGGAGAAG TGGTAATGCC
    AACTTCAACT CAAATATCAT AAAGTGGTTA TACTTTCCAG ACTTCATTGT GAGACCAAAC
    CCTGTCTTCC TTGTCTATGA TTTCATGTTG CTGCTGTGTG CATCCTTACA AAGGCAAACG
    TTTGAGGATG AAAATAAAGC TGCTGTGCGA ATCATGGCTG GAGACAACGT GGAAATTTGT
    ATGAATCTTG AAGCAGCATC TTTTAGCCAG CATAATCCTG TTCCAGACTT CATTCACTGC
    CGGTCCTACT TAGATATGTA TAAGGTGATA ATCTTTAGTT ACCTCTTCTG GTTTGTGCTT
    ACTATAATCT TCATAACGGG AACAACGAGG ATCAGCATAT TCTGTATGGG CTACTTGGTT
    GCCTGCTTTT ATTTCCTTCT CTTTGGTGGA GATCTGCTGC TGAAACCCAT CAGGAGCATC
    CTGCGCTACT GGGACTGGCT GATTGCATAC AATGTTTTTG TGATCACCAT GAAGAACATC
    CTCTCGATAG GAGCATGTGG CTACATTGAG TCTTTAATTC AAAATAGTTG CTGGTTGATT
    CAGGCATTTA GTCTGGCTTG CACAGTTAAA GGCTACCGTA TCCCAACCAA CAATGCAGAC
    TGTAAACTAC CTAGTGGAGA AGCAGGAATC ATCTGGGACA GTATATGTTT TGCCTTTCTA
    CTGCTGCAAA GAAGAGTCTT CATGAGTTAC TACTTCCTCC ACGTGGTTGC AGATATAAAA
    GCTTCTCAGA TCCTAGCATC AAGGGGTGCT GAGCTTTTCC AGGCCACAAT TGTTAAGGCA
    GTAAAGGCAA GAATTGAAGA GGAAAAAAAA TCAATGGATC AACTGAAAAG ACAAATGGAT
    CGCATCAAAG CCAGGCAGCA AAAATATAAA AAGGGTAAAG AGAGGATGCT AAGCATGACA
    CAGGATTCCA CAGAGGGGCC TGAAATTCGG AAGGTTTCGG AAGAAGATGA TGAAGGAGAA
    GCAGACAAAG AGAAAGCCAA GGGCAAAAAA AAGCTGTGGT GGCGGCCTTG GGTTGATCAT
    GCTTCCATGG TCAGAAGTGG AAATTATTAT TTGTTTGAGA CGGACAGTGA AGAGGAAGAG
    GAAGAAGAGA AAAAAGAAGA GGAAGAGCCT CGAAAAAAAT CTGCATTTCA GTTTGTTTAT
    CAGGCCTGGA TTACTGATCC TAAAACTGCG CTACGACAGA GGCGCAAGGA AAAGAAAAGC
    TTTCAAAAAG AGGAGAAGCG AAGGCGTAAA GTATATGGGG ATGGAAGTAC TGATGCAGAC
    TGCGAAGACA GTGAAGAAGA ACCTGTCAAG AAGAAGTCTG ATGGCCCAGA TAACATCATC
    AAGAGGATAT TTAATATTTT GAAGTTCACT TGGGTCCTTT TCCTGGCAAC ACTAGACAGT
    TTTACAGCTT GGCTTAATTC CATCTCAAGA GAACACATCG ATATCTCTAC TGTGCTGAGA
    ATCGAACGTT GTATGCTGAC CAGGGAGATT AAGAAGGGAA ACGTTCCTAC CCGGGAGAGC
    ATCCATATGT ATTACCAGAA CCATATGATG AAACTTTCTA AGGAGTCAGG ACTGGACTCT
    ATTGATAAAA ATCCTGGGCA AGCTTCTGGT TTGCAAACAT CTGAAAGAAT GGATAGTTTA
    GATTCAGCAG CTTCTCGAGA CAGTATCTCC AGCTGCTACA CTGAAGCAAC CATGCTGTTC
    TCAAGGCAGT CAACCCTTGA TGATTTAGAT GGACCAGACA CTGTTCCTAA AACAAGTGAG
    CGCGCTCGAC CTAGGCTTCG TAAAATGCAG AGCATGGATA TGTCCTCTTC ATCAGCTGAC
    AGTGGCAGTA TAGTGTCAAG CGAGCCTACA CAGGTCACCA TGCTCTATTC TCGTCAGGGG
    ACGACAGAAA CCATTGAGGA GGTAGAAGGA GAGCATGAAG AGGAAGGAGC TCATTCTGCT
    TCAAGGCAGG ATGAAGAAGA GGTGGAAGAT TATAGCCTGG GTTCAGAAGG AGCTGCATAT
    ACTCCCGACA CCGATGTACC ATTGTACTCC ACTGTGGACA GCAATGCAGA AGCACCACCT
    TCCTATAGCA AAGCTGTCAG CTTTGAACAC CTTCCTTTTG GCTCCCCAGA TGACTCAGCT
    GGAAAGAGTC TCATGATGGT GAGCCCAGAT GACAGCCGGA CGGACAGACT TAATGATGCA
    ATTCTCCCTC CATTGACGCA CGAGCTAACG GCTAGTGAGC TGCTTCTGAA CAAGATGTTT
    CATGATGATG AGCTAGAGGA ATCAGAGAAA TTCTATGTTG GTCAGCCTCG AGTCTTGCTT
    CTTATTTATG CGCTGTACAA TACTCTGGTG GCCCGGTCAG AAATGGTTTG CTATTTTGTC
    ATCATCCTTA ACCACATGAT CTCTGCCTCT ATGATAACGC TGGTGCTGCC CATCCTTATC
    TTTCTTTGGG CCATGCTGTC TGTCCCTCGA CCAAGCAAAC GTTTCTGGAT GACTGCCATT
    GTCTACACTG AGGTAGCCAT TGTCATCAAG TACTTCTTCC AGTTTGGCTT CTTTCCTTGG
    AATAAATATG TGGATTACAC AAAAGATAAA CCTTATCATC CACCCAATAT TATTGGGATA
    GAGAAGAAAG AGGGCTATGT GCACTATGAT CTTGTTCAGC TCCTTGCTTT ATTTTTCCAC
    AGATCCATTT TAAAGTGCCA TGGATTGTGG GATGAAGATG AGAAAGGGGA TAACTCCAGT
    AATAAAGGAG ATACTGATGA TGAGCTTTCT CTGCCGGGAG GCAGGAGAGA CTCTTCTGGG
    TCTCTGAAGT CGGTCAATCT TGCAGCATCA GTGGAGTCCA TCCATGTGCA CTTCCCTGAG
    CAGCAGACAG CAATCAGGAG GAAGAGCTCC AGCAGTGCAT CACAGCTTTC TCACAGATCC
    AGCTTCTCCT CACACAGATC TAAGAGAGGA AGCACCAGTA CAAGGAACAG CAGTCAGAAA
    GGGAGCAGTG TGCTAAGCAT CAAGCAAAAA TCTAGGAAAG AACTTCTTAT GGAAAAGTTT
    CGAGAGCAAA TGATCAAAGC CAAAGCCTTT ACAATTAAAA AGACTCTCCA AGTGTATGTG
    CCCATCAGAC AATTTTTCTA CAATCTTATT CATCCAGACT ACAGTGCAGT GACTGATGTA
    TATGTGCTGA TGTTCCTCGC AGATACAGTG GACTTCATCA TCATTGTCTT TGGATTTTGG
    GCTTTTGGGA AACACTCTGC AGCAGCAGAT ATTACCTCTT CATTATCAGA GGACCAGGTT
    CCAGAGGCAT TTTTGGTGAT GGTGCTCATT CAGTTTGGTA CCATGGTGGT AGACCGAGCG
    CTCTACCTCA AAAAGACAGT CATGGGAAAG GTCATCTTCC AGGTCATCCT TGTTTTTGGG
    ATTCATTTCT GGATGTTCTT TATCTTGCCT GGAGTGACAG AAAGAAAATT TAGCCAGAAC
    ACCGTTGCCC AGCTCTGGTA TTTTGTGAAA TGTGTTTATT TTGGCTTATC AGCATATCAG
    ATACGCTGCG GATATCCAAC TCGTGTTCTT GGCAACTTCC TCACAAAAAG TTATAACTAT
    GTGAACCTGT TCTTATTTCA AGGATTCCGC CTGGTTCCAT TTTTGACTGA GCTGAGAGCA
    GTAATGGACT GGGTTTGGAC AGATACCACT CTCAGTCTCT CCAGCTGGAT CTGTGTTGAA
    GATATCTATG CTCATATATT CATCCTGAAG TGCTGGCGAG AGTCAGAAAA GAGATATCCC
    CAACCAAGAG GCCAGAAGAA AAAGAAGGTT GTGAAGTATG GAATGGGTGG CATGATTATC
    GTCTTGCTGA TTTGTATTGT CTGGTTCCCA CTGCTCTTCA TGTCTTTAAT CAAATCTGTT
    GCAGGCATCA CCAACAAGCC TCTAGATGTA TCAATCACAA TAACTCTAGG AGGTTATCAG
    CCTATTTTTA CAATGAGTGC TCAACAGAGT CAGCTCAAGG ATTTGAATCA GACTGGTTTC
    AGTGCATTTC TGGGAAGCTA TAGGGGTAAC ACTGCTGCTT TGCAGTTTTT AGAGGGCTAT
    GGAAAAGAAG ATATAACCCT AGCAGATCTA GAAGGGAATT CAAACTCCTT ATGGACCATC
    AGCCCTCCCA GTAGAGAGAA AATGATACAG GGACTGCTGG ATTTTTCAGC TGAGTTCACT
    GTAGTACTTT CATGGAGCAT TCAAAGAAAT CTCACTCTTG GTGCCAAAGC AGAAATAGCA
    TCAGATAAAC TTACCTTTGG CCTACCAGAG AAAACCAGAA GAGATATTGC TACAATGATG
    TCTGGAAAGC CATTGGAAAA GGTAACATTA GAAACAGTGT ATCCATACTA CATCAAGGCT
    CCTAGTGATT CTCTGGCAAA ACCCATAAAG CAGCTATTGA CAGACTGCAG ATGGGAAAAT
    ATCACAGTTT CCCTGGTCAA AAATGTGTCT GAAGAGGGAG TTCGTGAGTG GTGGGTTTTG
    AATCAGCTTG GAAAAAGATA CAAAACAAAT GAAGAGAGCC TTGAACTCTT CATATTCAGT
    GATAAAGTCA GTCCTCCAAG CCTTGGATTC TTGGCTGGAT ATGGCATCAT GGGACTATAC
    GCGTCAGTCG TCCTGGTGAT AGGGAAGTTC GTCCGTGAGT TTTTCAGTGG GATTTCTCAC
    TCCATCATGT TTGAGGAGCT ACCCAATGTA GACCGAATCT TGAAGTTGTG TACTGACATT
    TTCTTAGTAC GAGAGACTGG AGAACTGGAA CTAGAAGAAG ACCTGTACGC CAAACTAATC
    TTCCTGTATC GCTCACCAGA GACTATGATC AAGTGGACTA GGGAAAAAAC TAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138028.1
    CDS52..7428
    Translation

    Target ORF information:

    RefSeq Version XM_015282542.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus family with sequence similarity 38, member B (FAM38B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGATCATCA AAAATCAAGA GTTACACTGG TATCACCATG CCAATCCAGT TCTACTGCTG 
    GTGATGTACT ACACCCTTGC AACTCTGATC CGCCTTTGGC TGCAAGAGCC CATTCAGGTA
    CCTGAGGAAG AGAAATCTGA GAGCAGGGAA GATGACAAAG AGATACCCTG CAGCCCAATT
    CCAATGACAG CAGAGAGAAG ACGCAGTCTG TGGTACGCAA GCCACTATAC AACTGATGAG
    AGAAAAGTTC TGCTGGTAAC AGTGAACGGG AATCCTGCTG ACTATCACAC CATCCACCCC
    TCACTGCCAT TAGAGAATGG TCCAGCCAAA GCAGACATGT ACTCAACACC ACAGTACAAA
    TGGGAGCCCT CGGATAACAT GTCAGAAAAG GAAGAGGAGG AAGTAGAGGA GGAAGAAGAA
    GTTCCTCAGG AGGAAAAAGA AAATGTTAAA TTGCATGCCC TGGTCTCTGT GTTCCAATTT
    ATCATGAAGC AAAGTTACAT TTGTGCTTTG ATAGCTATGA TGGCATGGAG TATCACGTAT
    CACAGCTGGT TGACTTTTGT GCTCTTGATC TGGTCTTGCA CGCTGTGGAT GATTCGAGAC
    CGAAGGAAAT ATGCTATGAT CAGTTCACCG TTCATGGTTT TCTATGGAAA TTTACTGCTA
    ATATTACAAT ATATATGGAG TATTGAGCTC AAGAATGATG AGCTTCCTCA AGTATCAGGT
    TTTTTAGAAA GAAAGGAACC TGGAGAGTTG GCATCAAAGA TTCTTTTCAC GATTACTTTC
    TGGCTACTGC TTAGGCAACA CCTCACTGAA CAGAAAGCAC TTCAGTTAAA AGAAGCTACT
    TTATCTGAGG TCAAAGTTGG AAGTGAAGAA GATGAAGAAA AAGAAGAGGG AGAGTTGCAA
    GAGGGAGAAG TGGCAGAAGA AGAGGAGGAG GAGGAGGAGG AACAGGAAGA TGATGATGAG
    GATGAGCAGG ATATTATGAA AGTTCTGGGG AAGCTGGTGA TGGCTATGTT CATCAAGTAC
    TGGATTTATG TCTGTGGAGG CATGTTCTTC TTCGTCAGCT TTGAGGGTAG AATTGTCATG
    TACAAAATCA TCTACATGGT ACTATTCCTG TTCTGTGTGG CCCTCTATCA GGTACACTAT
    GAATGGTGGA GAAAGATTCT GAAATACTTT TGGATGTCAG TTGTTGTTTA CACGATGTTG
    GTACTTATCT TCATCTACAC ATACCAGTTT GAGTCATTCC CTGGGTTGTG GAAAAATATG
    ACAGGCCTGG ATGAAAACAA ACTAGCAGAC CTTGGTTTAA AAAGATTTTC TGTGGCTGAG
    CTATTCACTC GCATCTTTAT ACCCACCTCC TTCCTACTGG CGTGTATCTT GCACCTTCAC
    TATTTCCATG ACCGTTTCCT CCAGCTCACT GATTTAAAAG CTGTAACCAG CAAACAGGAC
    AACACTATTT ACAGACTGGT GCATCAAGAT GGAAGCCTGC CTGACATAAC TATGATGAAT
    TTAACAGCCA GCAGTGAAAA GGAAGAGGAT AAAATGCTGA AAGAGGCTGG TGAGAAAAGA
    ATGGAAGAGC CAGATGGGGA GGGAGGGAAA GGGAGGGCTG AAAAAGGAAA AGAAGAAGAA
    GAGGAAGATG AGGAGAAGGA AGATGATGAT GTAGACGATG AGTCAGAGGA AGAAGAAACT
    ACAGACCTAA GAAACAAGTG GCATCTGGTG ATTGACCGCC TTACAGTGCT GTTTTTGAAA
    TTCCTGGAGT ACTTTCATAA GATGCAAGTC TTTGTCTGGT GGCTTTTGGA GCTGCATATC
    ATCAAAATCG TCTCCTCTTA CATCATCTGG GTCACAGTAA AAGAGGTGTC TCTGTTTAAC
    TTTGTGTTTT TAATTGCTTG GGCTCTTGCT TTGCCATATG CTCAGTTTCG CCCATTGGCA
    TCAAGTATCT GCACTGTTTG GACATGTGTG ATTATTGTCT GCAAAATGTT GTACCAGCTC
    ACATCTATTG ATCCCAGCAC TTTTTCCAGT AACTGTACAT TGCCCAGAGA AAATGAAACA
    AAGGTTGATT TAGAAGAGCT CAAAACATCA GTCCTCTACA GTGGTCCGGT TGATCCTGCA
    GAATGGGTTG GATTGAGGAA GTCTTATCCC CTACTTTTAT ACTTAAGGAA TAACTTACTG
    ATGCTGGCTA TTCTGGCTTT TGAAGTTACA ATTTACCGTC ATCAAGAGTA CTACAGATGT
    CGAAATAACC TTACTGCACC TGTAACTAAA ACTATTTTCC ATGATATTAC AAGAGCACAT
    CTGGATGATG GACTGGTAAA CTGTGTCAAG TATTTCATAA ACTACTTCTT CTACAAGTTT
    GGTTTAGAGA CCTGTTTTCT TTTATCAGTG AATGTAATTG GTCAACGGAT GGATTTTTAT
    GCCATGATTC ATGCCTTCTG GTTGATTGCT GTATTGTATC GACGTAGAAG AAAAGCTATT
    GCAGAGATCT GGCCAAAGTA TTGCTGTTTT CTGGCATGCA TCATTACATT CCAGTATTTC
    CTTTGTATTG GCATTCCACC TGCTCCTTGT AAAGACTATC CATGGAGAAG TGGTAATGCC
    AACTTCAACT CAAATATCAT AAAGTGGTTA TACTTTCCAG ACTTCATTGT GAGACCAAAC
    CCTGTCTTCC TTGTCTATGA TTTCATGTTG CTGCTGTGTG CATCCTTACA AAGGCAAACG
    TTTGAGGATG AAAATAAAGC TGCTGTGCGA ATCATGGCTG GAGACAACGT GGAAATTTGT
    ATGAATCTTG AAGCAGCATC TTTTAGCCAG CATAATCCTG TTCCAGACTT CATTCACTGC
    CGGTCCTACT TAGATATGTA TAAGGTGATA ATCTTTAGTT ACCTCTTCTG GTTTGTGCTT
    ACTATAATCT TCATAACGGG AACAACGAGG ATCAGCATAT TCTGTATGGG CTACTTGGTT
    GCCTGCTTTT ATTTCCTTCT CTTTGGTGGA GATCTGCTGC TGAAACCCAT CAGGAGCATC
    CTGCGCTACT GGGACTGGCT GATTGCATAC AATGTTTTTG TGATCACCAT GAAGAACATC
    CTCTCGATAG GAGCATGTGG CTACATTGAG TCTTTAATTC AAAATAGTTG CTGGTTGATT
    CAGGCATTTA GTCTGGCTTG CACAGTTAAA GGCTACCGTA TCCCAACCAA CAATGCAGAC
    TGTAAACTAC CTAGTGGAGA AGCAGGAATC ATCTGGGACA GTATATGTTT TGCCTTTCTA
    CTGCTGCAAA GAAGAGTCTT CATGAGTTAC TACTTCCTCC ACGTGGTTGC AGATATAAAA
    GCTTCTCAGA TCCTAGCATC AAGGGGTGCT GAGCTTTTCC AGGCCACAAT TGTTAAGGCA
    GTAAAGGCAA GAATTGAAGA GGAAAAAAAA TCAATGGATC AACTGAAAAG ACAAATGGAT
    CGCATCAAAG CCAGGCAGCA AAAATATAAA AAGGGTAAAG AGAGGATGCT AAGCATGACA
    CAGGATTCCA CAGAGGGGCC TGAAATTCGG AAGGTTTCGG AAGAAGATGA TGAAGGAGAA
    GCAGACAAAG AGAAAGCCAA GGGCAAAAAA AAGCTGTGGT GGCGGCCTTG GGTTGATCAT
    GCTTCCATGG TCAGAAGTGG AAATTATTAT TTGTTTGAGA CGGACAGTGA AGAGGAAGAG
    GAAGAAGAGA AAAAAGAAGA GGAAGAGCCT CGAAAAAAAT CTGCATTTCA GTTTGTTTAT
    CAGGCCTGGA TTACTGATCC TAAAACTGCG CTACGACAGA GGCGCAAGGA AAAGAAAAGC
    TTTCAAAAAG AGGAGAAGCG AAGGCGTAAA GTATATGGGG ATGGAAGTAC TGATGCAGAC
    TGCGAAGACA GTGAAGAAGA ACCTGTCAAG AAGAAGTCTG ATGGCCCAGA TAACATCATC
    AAGAGGATAT TTAATATTTT GAAGTTCACT TGGGTCCTTT TCCTGGCAAC ACTAGACAGT
    TTTACAGCTT GGCTTAATTC CATCTCAAGA GAACACATCG ATATCTCTAC TGTGCTGAGA
    ATCGAACGTT GTATGCTGAC CAGGGAGATT AAGAAGGGAA ACGTTCCTAC CCGGGAGAGC
    ATCCATATGT ATTACCAGAA CCATATGATG AAACTTTCTA AGGAGTCAGG ACTGGACTCT
    ATTGATAAAA ATCCTGGGCA AGCTTCTGGT TTGCAAACAT CTGAAAGAAT GGATAGTTTA
    GATTCAGCAG CTTCTCGAGA CAGTATCTCC AGCTGCTACA CTGAAGCAAC CATGCTGTTC
    TCAAGGCAGT CAACCCTTGA TGATTTAGAT GGACCAGACA CTGTTCCTAA AACAAGTGAG
    CGCGCTCGAC CTAGGCTTCG TAAAATGCAG AGCATGGATA TGTCCTCTTC ATCAGCTGAC
    AGTGGCAGTA TAGTGTCAAG CGAGCCTACA CAGGTCACCA TGCTCTATTC TCGTCAGGGG
    ACGACAGAAA CCATTGAGGA GGTAGAAGGA GAGCATGAAG AGGAAGGAGC TCATTCTGCT
    TCAAGGCAGG ATGAAGAAGA GGTGGAAGAT TATAGCCTGG GTTCAGAAGG AGCTGCATAT
    ACTCCCGACA CCGATGTACC ATTGTACTCC ACTGTGGACA GCAATGCAGA AGCACCACCT
    TCCTATAGCA AAGCTGTCAG CTTTGAACAC CTTCCTTTTG GCTCCCCAGA TGACTCAGCT
    GGAAAGAGTC TCATGATGGT GAGCCCAGAT GACAGCCGGA CGGACAGACT TAATGATGCA
    ATTCTCCCTC CATTGACGCA CGAGCTAACG GCTAGTGAGC TGCTTCTGAA CAAGATGTTT
    CATGATGATG AGCTAGAGGA ATCAGAGAAA TTCTATGTTG GTCAGCCTCG AGTCTTGCTT
    CTTATTTATG CGCTGTACAA TACTCTGGTG GCCCGGTCAG AAATGGTTTG CTATTTTGTC
    ATCATCCTTA ACCACATGAT CTCTGCCTCT ATGATAACGC TGGTGCTGCC CATCCTTATC
    TTTCTTTGGG CCATGCTGTC TGTCCCTCGA CCAAGCAAAC GTTTCTGGAT GACTGCCATT
    GTCTACACTG AGGTAGCCAT TGTCATCAAG TACTTCTTCC AGTTTGGCTT CTTTCCTTGG
    AATAAATATG TGGATTACAC AAAAGATAAA CCTTATCATC CACCCAATAT TATTGGGATA
    GAGAAGAAAG AGGGCTATGT GCACTATGAT CTTGTTCAGC TCCTTGCTTT ATTTTTCCAC
    AGATCCATTT TAAAGTGCCA TGGATTGTGG GATGAAGATG AGAAAGGGGA TAACTCCAGT
    AATAAAGGAG ATACTGATGA TGAGCTTTCT CTGCCGGGAG GCAGGAGAGA CTCTTCTGGG
    TCTCTGAAGT CGGTCAATCT TGCAGCATCA GTGGAGTCCA TCCATGTGCA CTTCCCTGAG
    CAGCAGACAG CAATCAGGAG GAAGAGCTCC AGCAGTGCAT CACAGCTTTC TCACAGATCC
    AGCTTCTCCT CACACAGATC TAAGAGAGGA AGCACCAGTA CAAGGAACAG CAGTCAGAAA
    GGGAGCAGTG TGCTAAGCAT CAAGCAAAAA TCTAGGAAAG AACTTCTTAT GGAAAAGTTT
    CGAGAGCAAA TGATCAAAGC CAAAGCCTTT ACAATTAAAA AGACTCTCCA AGTGTATGTG
    CCCATCAGAC AATTTTTCTA CAATCTTATT CATCCAGACT ACAGTGCAGT GACTGATGTA
    TATGTGCTGA TGTTCCTCGC AGATACAGTG GACTTCATCA TCATTGTCTT TGGATTTTGG
    GCTTTTGGGA AACACTCTGC AGCAGCAGAT ATTACCTCTT CATTATCAGA GGACCAGGTT
    CCAGAGGCAT TTTTGGTGAT GGTGCTCATT CAGTTTGGTA CCATGGTGGT AGACCGAGCG
    CTCTACCTCA AAAAGACAGT CATGGGAAAG GTCATCTTCC AGGTCATCCT TGTTTTTGGG
    ATTCATTTCT GGATGTTCTT TATCTTGCCT GGAGTGACAG AAAGAAAATT TAGCCAGAAC
    ACCGTTGCCC AGCTCTGGTA TTTTGTGAAA TGTGTTTATT TTGGCTTATC AGCATATCAG
    ATACGCTGCG GATATCCAAC TCGTGTTCTT GGCAACTTCC TCACAAAAAG TTATAACTAT
    GTGAACCTGT TCTTATTTCA AGGATTCCGC CTGGTTCCAT TTTTGACTGA GCTGAGAGCA
    GTAATGGACT GGGTTTGGAC AGATACCACT CTCAGTCTCT CCAGCTGGAT CTGTGTTGAA
    GATATCTATG CTCATATATT CATCCTGAAG TGCTGGCGAG AGTCAGAAAA GAGATATCCC
    CAACCAAGAG GCCAGAAGAA AAAGAAGGTT GTGAAGTATG GAATGGGTGG CATGATTATC
    GTCTTGCTGA TTTGTATTGT CTGGTTCCCA CTGCTCTTCA TGTCTTTAAT CAAATCTGTT
    GCAGGCATCA CCAACAAGCC TCTAGATGTA TCAATCACAA TAACTCTAGG AGGTTATCAG
    CCTATTTTTA CAATGAGTGC TCAACAGAGT CAGCTCAAGG ATTTGAATCA GACTGGTTTC
    AGTGCATTTC TGGGAAGCTA TAGGGGTAAC ACTGCTGCTT TGCAGTTTTT AGAGGGCTAT
    GGAAAAGAAG ATATAACCCT AGCAGATCTA GAAGGGAATT CAAACTCCTT ATGGACCATC
    AGCCCTCCCA GTAGAGAGAA AATGATACAG GGACTGCTGG ATTTTTCAGC TGAGTTCACT
    GTAGTACTTT CATGGAGCAT TCAAAGAAAT CTCACTCTTG GTGCCAAAGC AGAAATAGCA
    TCAGATAAAC TTACCTTTGG CCTACCAGAG AAAACCAGAA GAGATATTGC TACAATGATG
    TCTGGAAAGC CATTGGAAAA GGTAACATTA GAAACAGTGT ATCCATACTA CATCAAGGCT
    CCTAGTGATT CTCTGGCAAA ACCCATAAAG CAGCTATTGA CAGACTGCAG ATGGGAAAAT
    ATCACAGTTT CCCTGGTCAA AAATGTGTCT GAAGAGGGAG TTCGTGAGTG GTGGGTTTTG
    AATCAGCTTG GAAAAAGATA CAAAACAAAT GAAGAGAGCC TTGAACTCTT CATATTCAGT
    GATAAAGTCA GTCCTCCAAG CCTTGGATTC TTGGCTGGAT ATGGCATCAT GGGACTATAC
    GCGTCAGTCG TCCTGGTGAT AGGGAAGTTC GTCCGTGAGT TTTTCAGTGG GATTTCTCAC
    TCCATCATGT TTGAGGAGCT ACCCAATGTA GACCGAATCT TGAAGTTGTG TACTGACATT
    TTCTTAGTAC GAGAGACTGG AGAACTGGAA CTAGAAGAAG ACCTGTACGC CAAACTAATC
    TTCCTGTATC GCTCACCAGA GACTATGATC AAGTGGACTA GGGAAAAAAC TAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32434
    Clone ID Related Accession (Same CDS sequence) XM_015282543.1
    Accession Version XM_015282543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455796.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGATCATCA AAAATCAAGA GTTACACTGG TATCACCATG CCAATCCAGT TCTACTGCTG 
    GTGATGTACT ACACCCTTGC AACTCTGATC CGCCTTTGGC TGCAAGAGCC CATTCAGGTA
    CCTGAGGAAG AGAAATCTGA GAGCAGGGAA GATGACAAAG AGATACCCTG CAGCCCAATT
    CCAATGACAG CAGAGAGAAG ACGCAGTCTG TGGTACGCAA GCCACTATAC AACTGATGAG
    AGAAAACTTC TGTCAATGAC CCAAGATGAA TACAAGCCAT CGGATGTTCT GCTGGTAACA
    GTGAACGGGA ATCCTGCTGA CTATCACACC ATCCACCCCT CACTGCCATT AGAGAATGGT
    CCAGCCAAAG CAGACATGTA CTCAACACCA CAGTACAAAT GGGAGCCCTC GGATAACATG
    TCAGAAAAGG AAGAGGAGGA AGTAGAGGAG GAAGAAGAAG TTCCTCAGGA GGAAAAAGAA
    AATGTTAAAT TGCATGCCCT GGTCTCTGTG TTCCAATTTA TCATGAAGCA AAGTTACATT
    TGTGCTTTGA TAGCTATGAT GGCATGGAGT ATCACGTATC ACAGCTGGTT GACTTTTGTG
    CTCTTGATCT GGTCTTGCAC GCTGTGGATG ATTCGAGACC GAAGGAAATA TGCTATGATC
    AGTTCACCGT TCATGGTTTT CTATGGAAAT TTACTGCTAA TATTACAATA TATATGGAGT
    ATTGAGCTCA AGAATGATGA GCTTCCTCAA GTATCAGGTT TTTTAGAAAG AAAGGAACCT
    GGAGAGTTGG CATCAAAGAT TCTTTTCACG ATTACTTTCT GGCTACTGCT TAGGCAACAC
    CTCACTGAAC AGAAAGCACT TCAGTTAAAA GAAGCTACTT TATCTGAGGT CAAAGTTGGA
    AGTGAAGAAG ATGAAGAAAA AGAAGAGGGA GAGTTGCAAG AGGGAGAAGT GGCAGAAGAA
    GAGGAGGAGG AGGAGGAGGA ACAGGAAGAT GATGATGAGG ATGAGCAGGA TATTATGAAA
    GTTCTGGGGA AGCTGGTGAT GGCTATGTTC ATCAAGTACT GGATTTATGT CTGTGGAGGC
    ATGTTCTTCT TCGTCAGCTT TGAGGGTAGA ATTGTCATGT ACAAAATCAT CTACATGGTA
    CTATTCCTGT TCTGTGTGGC CCTCTATCAG GTACACTATG AATGGTGGAG AAAGATTCTG
    AAATACTTTT GGATGTCAGT TGTTGTTTAC ACGATGTTGG TACTTATCTT CATCTACACA
    TACCAGTTTG AGTCATTCCC TGGGTTGTGG AAAAATATGA CAGGCCTGGA TGAAAACAAA
    CTAGCAGACC TTGGTTTAAA AAGATTTTCT GTGGCTGAGC TATTCACTCG CATCTTTATA
    CCCACCTCCT TCCTACTGGC GTGTATCTTG CACCTTCACT ATTTCCATGA CCGTTTCCTC
    CAGCTCACTG ATTTAAAAGC TGTAACCAGC AAACAGGACA ACACTATTTA CAGACTGGTG
    CATCAAGATG GAAGCCTGCC TGACATAACT ATGATGAATT TAACAGCCAG CAGTGAAAAG
    GAAGAGGATA AAATGCTGAA AGAGGCTGGT GAGAAAAGAA TGGAAGAGCC AGATGGGGAG
    GGAGGGAAAG GGAGGGCTGA AAAAGGAAAA GAAGAAGAAG AGGAAGATGA GGAGAAGGAA
    GATGATGATG TAGACGATGA GTCAGAGGAA GAAGAAACTA CAGACCTAAG AAACAAGTGG
    CATCTGGTGA TTGACCGCCT TACAGTGCTG TTTTTGAAAT TCCTGGAGTA CTTTCATAAG
    ATGCAAGTCT TTGTCTGGTG GCTTTTGGAG CTGCATATCA TCAAAATCGT CTCCTCTTAC
    ATCATCTGGG TCACAGTAAA AGAGGTGTCT CTGTTTAACT TTGTGTTTTT AATTGCTTGG
    GCTCTTGCTT TGCCATATGC TCAGTTTCGC CCATTGGCAT CAAGTATCTG CACTGTTTGG
    ACATGTGTGA TTATTGTCTG CAAAATGTTG TACCAGCTCA CATCTATTGA TCCCAGCACT
    TTTTCCAGTA ACTGTACATT GCCCAGAGAA AATGAAACAA AGGTTGATTT AGAAGAGCTC
    AAAACATCAG TCCTCTACAG TGGTCCGGTT GATCCTGCAG AATGGGTTGG ATTGAGGAAG
    TCTTATCCCC TACTTTTATA CTTAAGGAAT AACTTACTGA TGCTGGCTAT TCTGGCTTTT
    GAAGTTACAA TTTACCGTCA TCAAGAGTAC TACAGATGTC GAAATAACCT TACTGCACCT
    GTAACTAAAA CTATTTTCCA TGATATTACA AGAGCACATC TGGATGATGG ACTGGTAAAC
    TGTGTCAAGT ATTTCATAAA CTACTTCTTC TACAAGTTTG GTTTAGAGAC CTGTTTTCTT
    TTATCAGTGA ATGTAATTGG TCAACGGATG GATTTTTATG CCATGATTCA TGCCTTCTGG
    TTGATTGCTG TATTGTATCG ACGTAGAAGA AAAGCTATTG CAGAGATCTG GCCAAAGTAT
    TGCTGTTTTC TGGCATGCAT CATTACATTC CAGTATTTCC TTTGTATTGG CATTCCACCT
    GCTCCTTGTA AAGACTATCC ATGGAGAAGT GGTAATGCCA ACTTCAACTC AAATATCATA
    AAGTGGTTAT ACTTTCCAGA CTTCATTGTG AGACCAAACC CTGTCTTCCT TGTCTATGAT
    TTCATGTTGC TGCTGTGTGC ATCCTTACAA AGGCAAACGT TTGAGGATGA AAATAAAGCT
    GCTGTGCGAA TCATGGCTGG AGACAACGTG GAAATTTGTA TGAATCTTGA AGCAGCATCT
    TTTAGCCAGC ATAATCCTGT TCCAGACTTC ATTCACTGCC GGTCCTACTT AGATATGTAT
    AAGGTGATAA TCTTTAGTTA CCTCTTCTGG TTTGTGCTTA CTATAATCTT CATAACGGGA
    ACAACGAGGA TCAGCATATT CTGTATGGGC TACTTGGTTG CCTGCTTTTA TTTCCTTCTC
    TTTGGTGGAG ATCTGCTGCT GAAACCCATC AGGAGCATCC TGCGCTACTG GGACTGGCTG
    ATTGCATACA ATGTTTTTGT GATCACCATG AAGAACATCC TCTCGATAGG AGCATGTGGC
    TACATTGAGT CTTTAATTCA AAATAGTTGC TGGTTGATTC AGGCATTTAG TCTGGCTTGC
    ACAGTTAAAG GCTACCGTAT CCCAACCAAC AATGCAGACT GTAAACTACC TAGTGGAGAA
    GCAGGAATCA TCTGGGACAG TATATGTTTT GCCTTTCTAC TGCTGCAAAG AAGAGTCTTC
    ATGAGTTACT ACTTCCTCCA CGTGGTTGCA GATATAAAAG CTTCTCAGAT CCTAGCATCA
    AGGGGTGCTG AGCTTTTCCA GGCCACAATT GTTAAGGCAG TAAAGGCAAG AATTGAAGAG
    GAAAAAAAAT CAATGGATCA ACTGAAAAGA CAAATGGATC GCATCAAAGC CAGGCAGCAA
    AAATATAAAA AGGGTAAAGA GAGGATGCTA AGCATGACAC AGGATTCCAC AGAGGGGCCT
    GAAATTCGGA AGGTTTCGGA AGAAGATGAT GAAGGAGAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCTGTGGTG GCGGCCTTGG GTTGATCATG CTTCCATGGT CAGAAGTGGA
    AATTATTATT TGTTTGAGAC GGACAGTGAA GAGGAAGAGG AAGAAGAGAA AAAAGAAGAG
    GAAGAGCCTC GAAAAAAATC TGCATTTCAG TTTGTTTATC AGGCCTGGAT TACTGATCCT
    AAAACTGCGC TACGACAGAG GCGCAAGGAA AAGAAAAGCT TTCAAAAAGA GGAGAAGCGA
    AGGCGTAAAG TATATGGGGA TGGAAGTACT GATGCAGACT GCGAAGACAG TGAAGAAGAA
    CCTGTCAAGA AGAAGTCTGA TGGCCCAGAT AACATCATCA AGAGGATATT TAATATTTTG
    AAGTTCACTT GGGTCCTTTT CCTGGCAACA CTAGACAGTT TTACAGCTTG GCTTAATTCC
    ATCTCAAGAG AACACATCGA TATCTCTACT GTGCTGAGAA TCGAACGTTG TATGCTGACC
    AGGGAGATTA AGAAGGGAAA CGTTCCTACC CGGGAGAGCA TCCATATGTA TTACCAGAAC
    CATATGATGA AACTTTCTAA GGAGTCAGGA CTGGACTCTA TTGATAAAAA TCCTGGGCAA
    GCTTCTGGTT TGCAAACATC TGAAAGAATG GATAGTTTAG ATTCAGCAGC TTCTCGAGAC
    AGTATCTCCA GCGAGCCTAC ACAGGTCACC ATGCTCTATT CTCGTCAGGG GACGACAGAA
    ACCATTGAGG AGGTAGAAGG AGAGCATGAA GAGGAAGGAG CTCATTCTGC TTCAAGGCAG
    GATGAAGAAG AGGTGGAAGA TTATAGCCTG GGTTCAGAAG GAGCTGCATA TACTCCCGAC
    ACCGATGTAC CATTGTACTC CACTGTGGAC AGCAATGCAG AAGCACCACC TTCCTATAGC
    AAAGCTGTCA GCTTTGAACA CCTTCCTTTT GGCTCCCCAG ATGACTCAGC TGGAAAGAGT
    CTCATGATGG TGAGCCCAGA TGACAGCCGG ACGGACAGAC TTAATGATGC AATTCTCCCT
    CCATTGACGC ACGAGCTAAC GGCTAGTGAG CTGCTTCTGA ACAAGATGTT TCATGATGAT
    GAGCTAGAGG AATCAGAGAA ATTCTATGTT GGTCAGCCTC GAGTCTTGCT TCTTATTTAT
    GCGCTGTACA ATACTCTGGT GGCCCGGTCA GAAATGGTTT GCTATTTTGT CATCATCCTT
    AACCACATGA TCTCTGCCTC TATGATAACG CTGGTGCTGC CCATCCTTAT CTTTCTTTGG
    GCCATGCTGT CTGTCCCTCG ACCAAGCAAA CGTTTCTGGA TGACTGCCAT TGTCTACACT
    GAGGTAGCCA TTGTCATCAA GTACTTCTTC CAGTTTGGCT TCTTTCCTTG GAATAAATAT
    GTGGATTACA CAAAAGATAA ACCTTATCAT CCACCCAATA TTATTGGGAT AGAGAAGAAA
    GAGGGCTATG TGCACTATGA TCTTGTTCAG CTCCTTGCTT TATTTTTCCA CAGATCCATT
    TTAAAGTGCC ATGGATTGTG GGATGAAGAT GAGAAAGGGG ATAACTCCAG TAATAAAGGA
    GATACTGATG ATGAGCTTTC TCTGCCGGGA GGCAGGAGAG ACTCTTCTGG GTCTCTGAAG
    TCGGTCAATC TTGCAGCATC AGTGGAGTCC ATCCATGTGC ACTTCCCTGA GCAGCAGACA
    GCAATCAGGA GGAAGAGCTC CAGCAGTGCA TCACAGCTTT CTCACAGATC CAGCTTCTCC
    TCACACAGAT CTAAGAGAGG AAGCACCAGT ACAAGGAACA GCAGTCAGAA AGGGAGCAGT
    GTGCTAAGCA TCAAGCAAAA ATCTAGGAAA GAACTTCTTA TGGAAAAGTT TCGAGAGCAA
    ATGATCAAAG CCAAAGCCTT TACAATTAAA AAGACTCTCC AAGTGTATGT GCCCATCAGA
    CAATTTTTCT ACAATCTTAT TCATCCAGAC TACAGTGCAG TGACTGATGT ATATGTGCTG
    ATGTTCCTCG CAGATACAGT GGACTTCATC ATCATTGTCT TTGGATTTTG GGCTTTTGGG
    AAACACTCTG CAGCAGCAGA TATTACCTCT TCATTATCAG AGGACCAGGT TCCAGAGGCA
    TTTTTGGTGA TGGTGCTCAT TCAGTTTGGT ACCATGGTGG TAGACCGAGC GCTCTACCTC
    AAAAAGACAG TCATGGGAAA GGTCATCTTC CAGGTCATCC TTGTTTTTGG GATTCATTTC
    TGGATGTTCT TTATCTTGCC TGGAGTGACA GAAAGAAAAT TTAGCCAGAA CACCGTTGCC
    CAGCTCTGGT ATTTTGTGAA ATGTGTTTAT TTTGGCTTAT CAGCATATCA GATACGCTGC
    GGATATCCAA CTCGTGTTCT TGGCAACTTC CTCACAAAAA GTTATAACTA TGTGAACCTG
    TTCTTATTTC AAGGATTCCG CCTGGTTCCA TTTTTGACTG AGCTGAGAGC AGTAATGGAC
    TGGGTTTGGA CAGATACCAC TCTCAGTCTC TCCAGCTGGA TCTGTGTTGA AGATATCTAT
    GCTCATATAT TCATCCTGAA GTGCTGGCGA GAGTCAGAAA AGAGATATCC CCAACCAAGA
    GGCCAGAAGA AAAAGAAGGT TGTGAAGTAT GGAATGGGTG GCATGATTAT CGTCTTGCTG
    ATTTGTATTG TCTGGTTCCC ACTGCTCTTC ATGTCTTTAA TCAAATCTGT TGCAGGCATC
    ACCAACAAGC CTCTAGATGT ATCAATCACA ATAACTCTAG GAGGTTATCA GCCTATTTTT
    ACAATGAGTG CTCAACAGAG TCAGCTCAAG GATTTGAATC AGACTGGTTT CAGTGCATTT
    CTGGGAAGCT ATAGGGGTAA CACTGCTGCT TTGCAGTTTT TAGAGGGCTA TGGAAAAGAA
    GATATAACCC TAGCAGATCT AGAAGGGAAT TCAAACTCCT TATGGACCAT CAGCCCTCCC
    AGTAGAGAGA AAATGATACA GGGACTGCTG GATTTTTCAG CTGAGTTCAC TGTAGTACTT
    TCATGGAGCA TTCAAAGAAA TCTCACTCTT GGTGCCAAAG CAGAAATAGC ATCAGATAAA
    CTTACCTTTG GCCTACCAGA GAAAACCAGA AGAGATATTG CTACAATGAT GTCTGGAAAG
    CCATTGGAAA AGGTAACATT AGAAACAGTG TATCCATACT ACATCAAGGC TCCTAGTGAT
    TCTCTGGCAA AACCCATAAA GCAGCTATTG ACAGACTGCA GATGGGAAAA TATCACAGTT
    TCCCTGGTCA AAAATGTGTC TGAAGAGGGA GTTCGTGAGT GGTGGGTTTT GAATCAGCTT
    GGAAAAAGAT ACAAAACAAA TGAAGAGAGC CTTGAACTCT TCATATTCAG TGATAAAGTC
    AGTCCTCCAA GCCTTGGATT CTTGGCTGGA TATGGCATCA TGGGACTATA CGCGTCAGTC
    GTCCTGGTGA TAGGGAAGTT CGTCCGTGAG TTTTTCAGTG GGATTTCTCA CTCCATCATG
    TTTGAGGAGC TACCCAATGT AGACCGAATC TTGAAGTTGT GTACTGACAT TTTCTTAGTA
    CGAGAGACTG GAGAACTGGA ACTAGAAGAA GACCTGTACG CCAAACTAAT CTTCCTGTAT
    CGCTCACCAG AGACTATGAT CAAGTGGACT AGGGAAAAAA CTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138029.1
    CDS52..7299
    Translation

    Target ORF information:

    RefSeq Version XM_015282543.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus family with sequence similarity 38, member B (FAM38B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGATCATCA AAAATCAAGA GTTACACTGG TATCACCATG CCAATCCAGT TCTACTGCTG 
    GTGATGTACT ACACCCTTGC AACTCTGATC CGCCTTTGGC TGCAAGAGCC CATTCAGGTA
    CCTGAGGAAG AGAAATCTGA GAGCAGGGAA GATGACAAAG AGATACCCTG CAGCCCAATT
    CCAATGACAG CAGAGAGAAG ACGCAGTCTG TGGTACGCAA GCCACTATAC AACTGATGAG
    AGAAAACTTC TGTCAATGAC CCAAGATGAA TACAAGCCAT CGGATGTTCT GCTGGTAACA
    GTGAACGGGA ATCCTGCTGA CTATCACACC ATCCACCCCT CACTGCCATT AGAGAATGGT
    CCAGCCAAAG CAGACATGTA CTCAACACCA CAGTACAAAT GGGAGCCCTC GGATAACATG
    TCAGAAAAGG AAGAGGAGGA AGTAGAGGAG GAAGAAGAAG TTCCTCAGGA GGAAAAAGAA
    AATGTTAAAT TGCATGCCCT GGTCTCTGTG TTCCAATTTA TCATGAAGCA AAGTTACATT
    TGTGCTTTGA TAGCTATGAT GGCATGGAGT ATCACGTATC ACAGCTGGTT GACTTTTGTG
    CTCTTGATCT GGTCTTGCAC GCTGTGGATG ATTCGAGACC GAAGGAAATA TGCTATGATC
    AGTTCACCGT TCATGGTTTT CTATGGAAAT TTACTGCTAA TATTACAATA TATATGGAGT
    ATTGAGCTCA AGAATGATGA GCTTCCTCAA GTATCAGGTT TTTTAGAAAG AAAGGAACCT
    GGAGAGTTGG CATCAAAGAT TCTTTTCACG ATTACTTTCT GGCTACTGCT TAGGCAACAC
    CTCACTGAAC AGAAAGCACT TCAGTTAAAA GAAGCTACTT TATCTGAGGT CAAAGTTGGA
    AGTGAAGAAG ATGAAGAAAA AGAAGAGGGA GAGTTGCAAG AGGGAGAAGT GGCAGAAGAA
    GAGGAGGAGG AGGAGGAGGA ACAGGAAGAT GATGATGAGG ATGAGCAGGA TATTATGAAA
    GTTCTGGGGA AGCTGGTGAT GGCTATGTTC ATCAAGTACT GGATTTATGT CTGTGGAGGC
    ATGTTCTTCT TCGTCAGCTT TGAGGGTAGA ATTGTCATGT ACAAAATCAT CTACATGGTA
    CTATTCCTGT TCTGTGTGGC CCTCTATCAG GTACACTATG AATGGTGGAG AAAGATTCTG
    AAATACTTTT GGATGTCAGT TGTTGTTTAC ACGATGTTGG TACTTATCTT CATCTACACA
    TACCAGTTTG AGTCATTCCC TGGGTTGTGG AAAAATATGA CAGGCCTGGA TGAAAACAAA
    CTAGCAGACC TTGGTTTAAA AAGATTTTCT GTGGCTGAGC TATTCACTCG CATCTTTATA
    CCCACCTCCT TCCTACTGGC GTGTATCTTG CACCTTCACT ATTTCCATGA CCGTTTCCTC
    CAGCTCACTG ATTTAAAAGC TGTAACCAGC AAACAGGACA ACACTATTTA CAGACTGGTG
    CATCAAGATG GAAGCCTGCC TGACATAACT ATGATGAATT TAACAGCCAG CAGTGAAAAG
    GAAGAGGATA AAATGCTGAA AGAGGCTGGT GAGAAAAGAA TGGAAGAGCC AGATGGGGAG
    GGAGGGAAAG GGAGGGCTGA AAAAGGAAAA GAAGAAGAAG AGGAAGATGA GGAGAAGGAA
    GATGATGATG TAGACGATGA GTCAGAGGAA GAAGAAACTA CAGACCTAAG AAACAAGTGG
    CATCTGGTGA TTGACCGCCT TACAGTGCTG TTTTTGAAAT TCCTGGAGTA CTTTCATAAG
    ATGCAAGTCT TTGTCTGGTG GCTTTTGGAG CTGCATATCA TCAAAATCGT CTCCTCTTAC
    ATCATCTGGG TCACAGTAAA AGAGGTGTCT CTGTTTAACT TTGTGTTTTT AATTGCTTGG
    GCTCTTGCTT TGCCATATGC TCAGTTTCGC CCATTGGCAT CAAGTATCTG CACTGTTTGG
    ACATGTGTGA TTATTGTCTG CAAAATGTTG TACCAGCTCA CATCTATTGA TCCCAGCACT
    TTTTCCAGTA ACTGTACATT GCCCAGAGAA AATGAAACAA AGGTTGATTT AGAAGAGCTC
    AAAACATCAG TCCTCTACAG TGGTCCGGTT GATCCTGCAG AATGGGTTGG ATTGAGGAAG
    TCTTATCCCC TACTTTTATA CTTAAGGAAT AACTTACTGA TGCTGGCTAT TCTGGCTTTT
    GAAGTTACAA TTTACCGTCA TCAAGAGTAC TACAGATGTC GAAATAACCT TACTGCACCT
    GTAACTAAAA CTATTTTCCA TGATATTACA AGAGCACATC TGGATGATGG ACTGGTAAAC
    TGTGTCAAGT ATTTCATAAA CTACTTCTTC TACAAGTTTG GTTTAGAGAC CTGTTTTCTT
    TTATCAGTGA ATGTAATTGG TCAACGGATG GATTTTTATG CCATGATTCA TGCCTTCTGG
    TTGATTGCTG TATTGTATCG ACGTAGAAGA AAAGCTATTG CAGAGATCTG GCCAAAGTAT
    TGCTGTTTTC TGGCATGCAT CATTACATTC CAGTATTTCC TTTGTATTGG CATTCCACCT
    GCTCCTTGTA AAGACTATCC ATGGAGAAGT GGTAATGCCA ACTTCAACTC AAATATCATA
    AAGTGGTTAT ACTTTCCAGA CTTCATTGTG AGACCAAACC CTGTCTTCCT TGTCTATGAT
    TTCATGTTGC TGCTGTGTGC ATCCTTACAA AGGCAAACGT TTGAGGATGA AAATAAAGCT
    GCTGTGCGAA TCATGGCTGG AGACAACGTG GAAATTTGTA TGAATCTTGA AGCAGCATCT
    TTTAGCCAGC ATAATCCTGT TCCAGACTTC ATTCACTGCC GGTCCTACTT AGATATGTAT
    AAGGTGATAA TCTTTAGTTA CCTCTTCTGG TTTGTGCTTA CTATAATCTT CATAACGGGA
    ACAACGAGGA TCAGCATATT CTGTATGGGC TACTTGGTTG CCTGCTTTTA TTTCCTTCTC
    TTTGGTGGAG ATCTGCTGCT GAAACCCATC AGGAGCATCC TGCGCTACTG GGACTGGCTG
    ATTGCATACA ATGTTTTTGT GATCACCATG AAGAACATCC TCTCGATAGG AGCATGTGGC
    TACATTGAGT CTTTAATTCA AAATAGTTGC TGGTTGATTC AGGCATTTAG TCTGGCTTGC
    ACAGTTAAAG GCTACCGTAT CCCAACCAAC AATGCAGACT GTAAACTACC TAGTGGAGAA
    GCAGGAATCA TCTGGGACAG TATATGTTTT GCCTTTCTAC TGCTGCAAAG AAGAGTCTTC
    ATGAGTTACT ACTTCCTCCA CGTGGTTGCA GATATAAAAG CTTCTCAGAT CCTAGCATCA
    AGGGGTGCTG AGCTTTTCCA GGCCACAATT GTTAAGGCAG TAAAGGCAAG AATTGAAGAG
    GAAAAAAAAT CAATGGATCA ACTGAAAAGA CAAATGGATC GCATCAAAGC CAGGCAGCAA
    AAATATAAAA AGGGTAAAGA GAGGATGCTA AGCATGACAC AGGATTCCAC AGAGGGGCCT
    GAAATTCGGA AGGTTTCGGA AGAAGATGAT GAAGGAGAAG CAGACAAAGA GAAAGCCAAG
    GGCAAAAAAA AGCTGTGGTG GCGGCCTTGG GTTGATCATG CTTCCATGGT CAGAAGTGGA
    AATTATTATT TGTTTGAGAC GGACAGTGAA GAGGAAGAGG AAGAAGAGAA AAAAGAAGAG
    GAAGAGCCTC GAAAAAAATC TGCATTTCAG TTTGTTTATC AGGCCTGGAT TACTGATCCT
    AAAACTGCGC TACGACAGAG GCGCAAGGAA AAGAAAAGCT TTCAAAAAGA GGAGAAGCGA
    AGGCGTAAAG TATATGGGGA TGGAAGTACT GATGCAGACT GCGAAGACAG TGAAGAAGAA
    CCTGTCAAGA AGAAGTCTGA TGGCCCAGAT AACATCATCA AGAGGATATT TAATATTTTG
    AAGTTCACTT GGGTCCTTTT CCTGGCAACA CTAGACAGTT TTACAGCTTG GCTTAATTCC
    ATCTCAAGAG AACACATCGA TATCTCTACT GTGCTGAGAA TCGAACGTTG TATGCTGACC
    AGGGAGATTA AGAAGGGAAA CGTTCCTACC CGGGAGAGCA TCCATATGTA TTACCAGAAC
    CATATGATGA AACTTTCTAA GGAGTCAGGA CTGGACTCTA TTGATAAAAA TCCTGGGCAA
    GCTTCTGGTT TGCAAACATC TGAAAGAATG GATAGTTTAG ATTCAGCAGC TTCTCGAGAC
    AGTATCTCCA GCGAGCCTAC ACAGGTCACC ATGCTCTATT CTCGTCAGGG GACGACAGAA
    ACCATTGAGG AGGTAGAAGG AGAGCATGAA GAGGAAGGAG CTCATTCTGC TTCAAGGCAG
    GATGAAGAAG AGGTGGAAGA TTATAGCCTG GGTTCAGAAG GAGCTGCATA TACTCCCGAC
    ACCGATGTAC CATTGTACTC CACTGTGGAC AGCAATGCAG AAGCACCACC TTCCTATAGC
    AAAGCTGTCA GCTTTGAACA CCTTCCTTTT GGCTCCCCAG ATGACTCAGC TGGAAAGAGT
    CTCATGATGG TGAGCCCAGA TGACAGCCGG ACGGACAGAC TTAATGATGC AATTCTCCCT
    CCATTGACGC ACGAGCTAAC GGCTAGTGAG CTGCTTCTGA ACAAGATGTT TCATGATGAT
    GAGCTAGAGG AATCAGAGAA ATTCTATGTT GGTCAGCCTC GAGTCTTGCT TCTTATTTAT
    GCGCTGTACA ATACTCTGGT GGCCCGGTCA GAAATGGTTT GCTATTTTGT CATCATCCTT
    AACCACATGA TCTCTGCCTC TATGATAACG CTGGTGCTGC CCATCCTTAT CTTTCTTTGG
    GCCATGCTGT CTGTCCCTCG ACCAAGCAAA CGTTTCTGGA TGACTGCCAT TGTCTACACT
    GAGGTAGCCA TTGTCATCAA GTACTTCTTC CAGTTTGGCT TCTTTCCTTG GAATAAATAT
    GTGGATTACA CAAAAGATAA ACCTTATCAT CCACCCAATA TTATTGGGAT AGAGAAGAAA
    GAGGGCTATG TGCACTATGA TCTTGTTCAG CTCCTTGCTT TATTTTTCCA CAGATCCATT
    TTAAAGTGCC ATGGATTGTG GGATGAAGAT GAGAAAGGGG ATAACTCCAG TAATAAAGGA
    GATACTGATG ATGAGCTTTC TCTGCCGGGA GGCAGGAGAG ACTCTTCTGG GTCTCTGAAG
    TCGGTCAATC TTGCAGCATC AGTGGAGTCC ATCCATGTGC ACTTCCCTGA GCAGCAGACA
    GCAATCAGGA GGAAGAGCTC CAGCAGTGCA TCACAGCTTT CTCACAGATC CAGCTTCTCC
    TCACACAGAT CTAAGAGAGG AAGCACCAGT ACAAGGAACA GCAGTCAGAA AGGGAGCAGT
    GTGCTAAGCA TCAAGCAAAA ATCTAGGAAA GAACTTCTTA TGGAAAAGTT TCGAGAGCAA
    ATGATCAAAG CCAAAGCCTT TACAATTAAA AAGACTCTCC AAGTGTATGT GCCCATCAGA
    CAATTTTTCT ACAATCTTAT TCATCCAGAC TACAGTGCAG TGACTGATGT ATATGTGCTG
    ATGTTCCTCG CAGATACAGT GGACTTCATC ATCATTGTCT TTGGATTTTG GGCTTTTGGG
    AAACACTCTG CAGCAGCAGA TATTACCTCT TCATTATCAG AGGACCAGGT TCCAGAGGCA
    TTTTTGGTGA TGGTGCTCAT TCAGTTTGGT ACCATGGTGG TAGACCGAGC GCTCTACCTC
    AAAAAGACAG TCATGGGAAA GGTCATCTTC CAGGTCATCC TTGTTTTTGG GATTCATTTC
    TGGATGTTCT TTATCTTGCC TGGAGTGACA GAAAGAAAAT TTAGCCAGAA CACCGTTGCC
    CAGCTCTGGT ATTTTGTGAA ATGTGTTTAT TTTGGCTTAT CAGCATATCA GATACGCTGC
    GGATATCCAA CTCGTGTTCT TGGCAACTTC CTCACAAAAA GTTATAACTA TGTGAACCTG
    TTCTTATTTC AAGGATTCCG CCTGGTTCCA TTTTTGACTG AGCTGAGAGC AGTAATGGAC
    TGGGTTTGGA CAGATACCAC TCTCAGTCTC TCCAGCTGGA TCTGTGTTGA AGATATCTAT
    GCTCATATAT TCATCCTGAA GTGCTGGCGA GAGTCAGAAA AGAGATATCC CCAACCAAGA
    GGCCAGAAGA AAAAGAAGGT TGTGAAGTAT GGAATGGGTG GCATGATTAT CGTCTTGCTG
    ATTTGTATTG TCTGGTTCCC ACTGCTCTTC ATGTCTTTAA TCAAATCTGT TGCAGGCATC
    ACCAACAAGC CTCTAGATGT ATCAATCACA ATAACTCTAG GAGGTTATCA GCCTATTTTT
    ACAATGAGTG CTCAACAGAG TCAGCTCAAG GATTTGAATC AGACTGGTTT CAGTGCATTT
    CTGGGAAGCT ATAGGGGTAA CACTGCTGCT TTGCAGTTTT TAGAGGGCTA TGGAAAAGAA
    GATATAACCC TAGCAGATCT AGAAGGGAAT TCAAACTCCT TATGGACCAT CAGCCCTCCC
    AGTAGAGAGA AAATGATACA GGGACTGCTG GATTTTTCAG CTGAGTTCAC TGTAGTACTT
    TCATGGAGCA TTCAAAGAAA TCTCACTCTT GGTGCCAAAG CAGAAATAGC ATCAGATAAA
    CTTACCTTTG GCCTACCAGA GAAAACCAGA AGAGATATTG CTACAATGAT GTCTGGAAAG
    CCATTGGAAA AGGTAACATT AGAAACAGTG TATCCATACT ACATCAAGGC TCCTAGTGAT
    TCTCTGGCAA AACCCATAAA GCAGCTATTG ACAGACTGCA GATGGGAAAA TATCACAGTT
    TCCCTGGTCA AAAATGTGTC TGAAGAGGGA GTTCGTGAGT GGTGGGTTTT GAATCAGCTT
    GGAAAAAGAT ACAAAACAAA TGAAGAGAGC CTTGAACTCT TCATATTCAG TGATAAAGTC
    AGTCCTCCAA GCCTTGGATT CTTGGCTGGA TATGGCATCA TGGGACTATA CGCGTCAGTC
    GTCCTGGTGA TAGGGAAGTT CGTCCGTGAG TTTTTCAGTG GGATTTCTCA CTCCATCATG
    TTTGAGGAGC TACCCAATGT AGACCGAATC TTGAAGTTGT GTACTGACAT TTTCTTAGTA
    CGAGAGACTG GAGAACTGGA ACTAGAAGAA GACCTGTACG CCAAACTAAT CTTCCTGTAT
    CGCTCACCAG AGACTATGAT CAAGTGGACT AGGGAAAAAA CTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.