robo3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following robo3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa13448 NM_001272937.1
Latest version!
Drosophila melanogaster roundabout 3 (robo3), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OFa13448 NM_134748.4
Latest version!
Drosophila melanogaster roundabout 3 (robo3), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa13448
    Clone ID Related Accession (Same CDS sequence) NM_001272937.1 , NM_134748.4
    Accession Version NM_001272937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product roundabout 3, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTAC GTTTCGTATT CATCGTTTTC CTGCTCAAGT GGACCCATGC ACAAGGATCC 
    CATCCGCCGC GGATAGTGGA GCATCCCATA GATACGACAG TGCCGCGACA CGAGCCAGCC
    ACGCTTAATT GCAAAGCCGA GGGCAGCCCC ACGCCCACCA TCCAGTGGTA CAAGGATGGG
    GTGCCCCTGA AGATCCTGCC GGGCTCCCAC CGCATCACCC TGCCAGCCGG CGGATTGTTT
    TTCCTCAAGG TTGTCAATTC ACGTCGCGAA ACAGATGCGG GCATCTACTG GTGCGAGGCG
    AAGAACGAAC TGGGCGTGGC AAGGAGTCGG AACGCCACGT TGCAAGTGGC GGTTCTCCGC
    GATGAGTTCC GCCTGGAGCC GCAGAACACA CGGATCGCCC AGGGCGATAC CGCCCTCCTC
    GAGTGCGCCG CCCCTCGGGG AATCCCCGAG CCCACAGTCA CCTGGAAGAA GGGCGGCCAG
    AAATTGGATT TGGAGGGCTC GAAGCGAGTA CGCATCGTTG ACGGCGGCAA TTTGGCCATC
    CAGGATGCCC GACAGACTGA CGAGGGTCAG TACCAGTGCA TAGCCAAGAA TCCTGTCGGG
    GTGCGCGAGT CCTCGTTGGC TACACTCAAA GTGCACGTCA AGCCGTACAT CATCCGGGGT
    CCGCACGATC AAACGGTTTT GGAGGGCGCC TCGGTGACCT TTCCCTGTCG AGTGGGTGGT
    GACCCCATGC CGGACGTACT CTGGCTGCGA ACCGCCTCGG GTGGCAACAT GCCATTGGAT
    CGCGTGAGTG TCCTGGAGGA TCGGAGTCTG CGACTGGAGA GGGTCACCAT TGCGGATGAA
    GGCGAGTACA GTTGCGAGGC GGACAATGTT GTGGGCGCCA TCACCGCGAT GGGAACTTTG
    ACTGTTTACG CCCCCCCGAA ATTCATCCAA CGTCCTGCGA GCAAATCCGT GGAACTCGGC
    GCCGACACAT CGTTCGAGTG TCGAGCTATA GGAAACCCAA AGCCCACCAT CTTCTGGACC
    ATCAAGAACA ACAGCACGCT GATATTTCCC GGAGCCCCGC CCTTGGATCG CTTTCATAGC
    CTCAACACTG AGGAAGGTCA CTCGATACTC ACGTTGACCA GGTTCCAGAG AACGGACAAG
    GATCTGGTCA TCCTGTGCAA CGCCATGAAC GAGGTGGCCA GCATTACCTC AAGGGTGCAG
    CTAAGCCTGG ACTCTCAAGA GGATCGACCA CCGCCGATCA TAATCTCCGG TCCGGTTAAT
    CAAACCCTAC CCATCAAGTC CCTGGCCACA CTTCAGTGCA AGGCCATCGG TTTGCCCAGT
    CCCACTATTT CCTGGTATCG CGATGGAATA CCAGTGCAAC CCAGTTCCAA GCTGAACATT
    ACCACATCCG GGGACTTGAT AATATCGGAC CTCGATCGAC AACAGGATCA AGGCCTATAC
    ACCTGCGTGG CCAGCTCGCG GGCTGGAAAG TCCACTTGGA GTGGTTTCCT CCGGATAGAG
    TTGCCAACCA ATCCGAATAT TAAGTTCTAC AGAGCCCCGG AGCAGACTAA GTGTCCCAGT
    GCTCCAGGGC AACCGAAAAT TCTGAACGCC ACTGCCTCGG CACTGACCAT TGTTTGGCCA
    ACCAGTGACA AGGCGGGAGC ATCGTCATTC CTAGGGTACA GTGTGGAAAT GTACTGCACC
    AATCAGAGCA GGACTTGGAT ACCCATTGCA TCGCGTTTGA GTGAGCCGAT TTTCACGGTC
    GAGAGTTTGA CGCAGGGAGC GGCTTACATG TTCATTGTTC GAGCGGAAAA CTCACTGGGC
    TTCTCGCCAC CCTCCCCCAT ATCGGAACCC ATTACGGCGG GAAAACTTGT GGGCGTGCGG
    GATGGTAGTG AAAGCACCGG GACCTCACAG CTCCTCCTCA GCGATGTGGA GACCTTGCTG
    CAGGCCAACG ATGTTGTGGA GCTACTGGAG GCCAATGCCA GTGATTCGAC GACCGCTCGA
    CTGTCTTGGG ACATAGATAG TGGTCAGTAC ATCGAGGGCT TCTATCTGTA CGCCCGGGAA
    CTGCACTCCT CCGAGTACAA AATGGTTACA CTGCTCAACA AGGGTCAGGG GCTGAGCTCC
    TGTACGGTGC CTGGACTGGC GAAGGCCTCC ACATACGAGT TTTTTCTTGT GCCATTTTAC
    AAGAGCATCG TAGGCAAGCC CTCGAACTCG CGACGCATGA GAACTTTGGA AGATGTTCCG
    GAGGCACCAC CTTATGGCAT GGAAGCCATT CAATTCAATC GCACCTCGGT CTTCTTGAAG
    TGGTTACCTC CACAACCAAA TCGGACTCGC AACGGCATCC TCACCAGCTA CAATGTGGTC
    GTCAAAGGCC TGGACGTGCA CAATACGACG CGGATATTCA AAAATATGAC CATAGACGCG
    GCCGCGCCCA CCCTGCTCCT GGCCAACCTC ACCACAGGAG TCACCTACTA TATCGCAGTG
    GCGGCTGCCA CGCGGGTGGG AGTCGGACCC TTCAGCAAGC CTGCCGTGCT CCGCATCGAT
    GCACGCACCC AATCCCTGGA CACTGGCTAC ACGAGATACC CCATCAGTCG TGATATTGCC
    GATGACTTTT TAACGCAGAC CTGGTTTATC GTCCTGCTGG GCTCCATCAT TGCCATAATT
    GTGTTTCTAT TGGGCGCATT GGTTCTATTC AAGCGCTACC AATTTATCAA GCAGACCTCG
    CTGGGCAGTT TACATGGCAA TCACGCAATC GGAACCGTGC GAAAGTTTCC AACATTGCCG
    CTAAATGGAG GCGGAGCCGT CGGTGCCGGT GGCTCAAATT CGACAGCCGC AGCTGGGCAG
    AGTGGCCATG GCCTGTGGAT CGATCCCACC AGCGGCGTTT GGCGACAGGC AGCGGGTGGC
    GGCAATCCTG GCGGAGTGGG CTCGTGTACG ACAAAGGAGC AACTTCCGGG ATACGCACAG
    GCCACCGCCC AACAAGGACA ACAGCCCACG CTGCTACCCG ATTACGAGAG ACTATCACCG
    CTGAATATGC CCGATTACGC GGAAGTCGCC TGCTCGACAT TCAAAAGCCC CACACACGGA
    GCACCGCCGA TGGGTGGTGG TGGTGGTGGC CAGTCGGCCT CCCTGTACGA CAGCTGTGGG
    GCCTACGCTA CCACCAATGT GGTGGCCAAT GTAAAGCTCT ACCAGAACCG CTATGCGACA
    AAGCCGAACC AGAGCAGCGG CAGCAATAAC AACAATCACC AGGGCAATGA CTACCAGTCG
    ACCGGGATGT ACTCCGCTCC ACCGAGCGCC CACTACGGAT GCTTGGAGCC AAAGCAGCAG
    CAGCAGCAGC AGCAGCCCAA TCTGATGACC ACCTCCACGG CCAGCACGGC CATCTTGACG
    AACTCGCCGG CCAAGGTGAA GAAGATCAAC ATAACCGAGA ACAAAATGGA TCAGCTGGAG
    GGCAAGTCGG AGCGGACGAA CCCGTTCAAC CAGCAGCAGC AGCTCCTCCT GGCCAGCAAC
    GCCCTGAAGC AGGGACTGGG CGCCTATGCC AACACCACCT TGGCGGCTCA GATGGCCAGT
    GGAGGTGGAG CGGGCACCTT GAGGCGCCAG CGTCAGCCGA AGACGCTTTA CAAAAGCGAA
    AACAATATAC TGGGCAAGTC TGGTCTGCGG CAGAACACCA TGAATGCGAG TGTAGGTGCT
    CCCAGCAACG GGCAGATGGA CTTCCTGACC GGCGGTCCTC CGTCGGAGGG CGGAGACTTC
    TCCGGACTGG GCCTCTGCAA CTCCACCAAC CAGCTGCTTA ACGACTGGGC ATCCAGTGCC
    TCGATCGCAG CTCCAGGGGA TTATCATTTC GGCAGCAAGC AGCCCAGCAA GCAGCACCTC
    TACGTGAAGG CCAAGGATGG CACCTGGTCG GCCGTCAGTT CGGATGCCTA TCAGTCCTTC
    AAGCATCAGC AGCAGCACCA TCCCTTCCTG GCAGGATCAG GTGACAACAC CAAGTCCCTA
    GCTAGTGTAA ACAGCTTAGC TGGCGATAGC AAATTCCTGA GTAGTTTTGG CTCTAGTGCC
    AATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259866.1
    CDS475..4503
    Translation

    Target ORF information:

    RefSeq Version NM_001272937.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster roundabout 3 (robo3), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTAC GTTTCGTATT CATCGTTTTC CTGCTCAAGT GGACCCATGC ACAAGGATCC 
    CATCCGCCGC GGATAGTGGA GCATCCCATA GATACGACAG TGCCGCGACA CGAGCCAGCC
    ACGCTTAATT GCAAAGCCGA GGGCAGCCCC ACGCCCACCA TCCAGTGGTA CAAGGATGGG
    GTGCCCCTGA AGATCCTGCC GGGCTCCCAC CGCATCACCC TGCCAGCCGG CGGATTGTTT
    TTCCTCAAGG TTGTCAATTC ACGTCGCGAA ACAGATGCGG GCATCTACTG GTGCGAGGCG
    AAGAACGAAC TGGGCGTGGC AAGGAGTCGG AACGCCACGT TGCAAGTGGC GGTTCTCCGC
    GATGAGTTCC GCCTGGAGCC GCAGAACACA CGGATCGCCC AGGGCGATAC CGCCCTCCTC
    GAGTGCGCCG CCCCTCGGGG AATCCCCGAG CCCACAGTCA CCTGGAAGAA GGGCGGCCAG
    AAATTGGATT TGGAGGGCTC GAAGCGAGTA CGCATCGTTG ACGGCGGCAA TTTGGCCATC
    CAGGATGCCC GACAGACTGA CGAGGGTCAG TACCAGTGCA TAGCCAAGAA TCCTGTCGGG
    GTGCGCGAGT CCTCGTTGGC TACACTCAAA GTGCACGTCA AGCCGTACAT CATCCGGGGT
    CCGCACGATC AAACGGTTTT GGAGGGCGCC TCGGTGACCT TTCCCTGTCG AGTGGGTGGT
    GACCCCATGC CGGACGTACT CTGGCTGCGA ACCGCCTCGG GTGGCAACAT GCCATTGGAT
    CGCGTGAGTG TCCTGGAGGA TCGGAGTCTG CGACTGGAGA GGGTCACCAT TGCGGATGAA
    GGCGAGTACA GTTGCGAGGC GGACAATGTT GTGGGCGCCA TCACCGCGAT GGGAACTTTG
    ACTGTTTACG CCCCCCCGAA ATTCATCCAA CGTCCTGCGA GCAAATCCGT GGAACTCGGC
    GCCGACACAT CGTTCGAGTG TCGAGCTATA GGAAACCCAA AGCCCACCAT CTTCTGGACC
    ATCAAGAACA ACAGCACGCT GATATTTCCC GGAGCCCCGC CCTTGGATCG CTTTCATAGC
    CTCAACACTG AGGAAGGTCA CTCGATACTC ACGTTGACCA GGTTCCAGAG AACGGACAAG
    GATCTGGTCA TCCTGTGCAA CGCCATGAAC GAGGTGGCCA GCATTACCTC AAGGGTGCAG
    CTAAGCCTGG ACTCTCAAGA GGATCGACCA CCGCCGATCA TAATCTCCGG TCCGGTTAAT
    CAAACCCTAC CCATCAAGTC CCTGGCCACA CTTCAGTGCA AGGCCATCGG TTTGCCCAGT
    CCCACTATTT CCTGGTATCG CGATGGAATA CCAGTGCAAC CCAGTTCCAA GCTGAACATT
    ACCACATCCG GGGACTTGAT AATATCGGAC CTCGATCGAC AACAGGATCA AGGCCTATAC
    ACCTGCGTGG CCAGCTCGCG GGCTGGAAAG TCCACTTGGA GTGGTTTCCT CCGGATAGAG
    TTGCCAACCA ATCCGAATAT TAAGTTCTAC AGAGCCCCGG AGCAGACTAA GTGTCCCAGT
    GCTCCAGGGC AACCGAAAAT TCTGAACGCC ACTGCCTCGG CACTGACCAT TGTTTGGCCA
    ACCAGTGACA AGGCGGGAGC ATCGTCATTC CTAGGGTACA GTGTGGAAAT GTACTGCACC
    AATCAGAGCA GGACTTGGAT ACCCATTGCA TCGCGTTTGA GTGAGCCGAT TTTCACGGTC
    GAGAGTTTGA CGCAGGGAGC GGCTTACATG TTCATTGTTC GAGCGGAAAA CTCACTGGGC
    TTCTCGCCAC CCTCCCCCAT ATCGGAACCC ATTACGGCGG GAAAACTTGT GGGCGTGCGG
    GATGGTAGTG AAAGCACCGG GACCTCACAG CTCCTCCTCA GCGATGTGGA GACCTTGCTG
    CAGGCCAACG ATGTTGTGGA GCTACTGGAG GCCAATGCCA GTGATTCGAC GACCGCTCGA
    CTGTCTTGGG ACATAGATAG TGGTCAGTAC ATCGAGGGCT TCTATCTGTA CGCCCGGGAA
    CTGCACTCCT CCGAGTACAA AATGGTTACA CTGCTCAACA AGGGTCAGGG GCTGAGCTCC
    TGTACGGTGC CTGGACTGGC GAAGGCCTCC ACATACGAGT TTTTTCTTGT GCCATTTTAC
    AAGAGCATCG TAGGCAAGCC CTCGAACTCG CGACGCATGA GAACTTTGGA AGATGTTCCG
    GAGGCACCAC CTTATGGCAT GGAAGCCATT CAATTCAATC GCACCTCGGT CTTCTTGAAG
    TGGTTACCTC CACAACCAAA TCGGACTCGC AACGGCATCC TCACCAGCTA CAATGTGGTC
    GTCAAAGGCC TGGACGTGCA CAATACGACG CGGATATTCA AAAATATGAC CATAGACGCG
    GCCGCGCCCA CCCTGCTCCT GGCCAACCTC ACCACAGGAG TCACCTACTA TATCGCAGTG
    GCGGCTGCCA CGCGGGTGGG AGTCGGACCC TTCAGCAAGC CTGCCGTGCT CCGCATCGAT
    GCACGCACCC AATCCCTGGA CACTGGCTAC ACGAGATACC CCATCAGTCG TGATATTGCC
    GATGACTTTT TAACGCAGAC CTGGTTTATC GTCCTGCTGG GCTCCATCAT TGCCATAATT
    GTGTTTCTAT TGGGCGCATT GGTTCTATTC AAGCGCTACC AATTTATCAA GCAGACCTCG
    CTGGGCAGTT TACATGGCAA TCACGCAATC GGAACCGTGC GAAAGTTTCC AACATTGCCG
    CTAAATGGAG GCGGAGCCGT CGGTGCCGGT GGCTCAAATT CGACAGCCGC AGCTGGGCAG
    AGTGGCCATG GCCTGTGGAT CGATCCCACC AGCGGCGTTT GGCGACAGGC AGCGGGTGGC
    GGCAATCCTG GCGGAGTGGG CTCGTGTACG ACAAAGGAGC AACTTCCGGG ATACGCACAG
    GCCACCGCCC AACAAGGACA ACAGCCCACG CTGCTACCCG ATTACGAGAG ACTATCACCG
    CTGAATATGC CCGATTACGC GGAAGTCGCC TGCTCGACAT TCAAAAGCCC CACACACGGA
    GCACCGCCGA TGGGTGGTGG TGGTGGTGGC CAGTCGGCCT CCCTGTACGA CAGCTGTGGG
    GCCTACGCTA CCACCAATGT GGTGGCCAAT GTAAAGCTCT ACCAGAACCG CTATGCGACA
    AAGCCGAACC AGAGCAGCGG CAGCAATAAC AACAATCACC AGGGCAATGA CTACCAGTCG
    ACCGGGATGT ACTCCGCTCC ACCGAGCGCC CACTACGGAT GCTTGGAGCC AAAGCAGCAG
    CAGCAGCAGC AGCAGCCCAA TCTGATGACC ACCTCCACGG CCAGCACGGC CATCTTGACG
    AACTCGCCGG CCAAGGTGAA GAAGATCAAC ATAACCGAGA ACAAAATGGA TCAGCTGGAG
    GGCAAGTCGG AGCGGACGAA CCCGTTCAAC CAGCAGCAGC AGCTCCTCCT GGCCAGCAAC
    GCCCTGAAGC AGGGACTGGG CGCCTATGCC AACACCACCT TGGCGGCTCA GATGGCCAGT
    GGAGGTGGAG CGGGCACCTT GAGGCGCCAG CGTCAGCCGA AGACGCTTTA CAAAAGCGAA
    AACAATATAC TGGGCAAGTC TGGTCTGCGG CAGAACACCA TGAATGCGAG TGTAGGTGCT
    CCCAGCAACG GGCAGATGGA CTTCCTGACC GGCGGTCCTC CGTCGGAGGG CGGAGACTTC
    TCCGGACTGG GCCTCTGCAA CTCCACCAAC CAGCTGCTTA ACGACTGGGC ATCCAGTGCC
    TCGATCGCAG CTCCAGGGGA TTATCATTTC GGCAGCAAGC AGCCCAGCAA GCAGCACCTC
    TACGTGAAGG CCAAGGATGG CACCTGGTCG GCCGTCAGTT CGGATGCCTA TCAGTCCTTC
    AAGCATCAGC AGCAGCACCA TCCCTTCCTG GCAGGATCAG GTGACAACAC CAAGTCCCTA
    GCTAGTGTAA ACAGCTTAGC TGGCGATAGC AAATTCCTGA GTAGTTTTGG CTCTAGTGCC
    AATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13448
    Clone ID Related Accession (Same CDS sequence) NM_001272937.1 , NM_134748.4
    Accession Version NM_134748.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product roundabout 3, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134748.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTAC GTTTCGTATT CATCGTTTTC CTGCTCAAGT GGACCCATGC ACAAGGATCC 
    CATCCGCCGC GGATAGTGGA GCATCCCATA GATACGACAG TGCCGCGACA CGAGCCAGCC
    ACGCTTAATT GCAAAGCCGA GGGCAGCCCC ACGCCCACCA TCCAGTGGTA CAAGGATGGG
    GTGCCCCTGA AGATCCTGCC GGGCTCCCAC CGCATCACCC TGCCAGCCGG CGGATTGTTT
    TTCCTCAAGG TTGTCAATTC ACGTCGCGAA ACAGATGCGG GCATCTACTG GTGCGAGGCG
    AAGAACGAAC TGGGCGTGGC AAGGAGTCGG AACGCCACGT TGCAAGTGGC GGTTCTCCGC
    GATGAGTTCC GCCTGGAGCC GCAGAACACA CGGATCGCCC AGGGCGATAC CGCCCTCCTC
    GAGTGCGCCG CCCCTCGGGG AATCCCCGAG CCCACAGTCA CCTGGAAGAA GGGCGGCCAG
    AAATTGGATT TGGAGGGCTC GAAGCGAGTA CGCATCGTTG ACGGCGGCAA TTTGGCCATC
    CAGGATGCCC GACAGACTGA CGAGGGTCAG TACCAGTGCA TAGCCAAGAA TCCTGTCGGG
    GTGCGCGAGT CCTCGTTGGC TACACTCAAA GTGCACGTCA AGCCGTACAT CATCCGGGGT
    CCGCACGATC AAACGGTTTT GGAGGGCGCC TCGGTGACCT TTCCCTGTCG AGTGGGTGGT
    GACCCCATGC CGGACGTACT CTGGCTGCGA ACCGCCTCGG GTGGCAACAT GCCATTGGAT
    CGCGTGAGTG TCCTGGAGGA TCGGAGTCTG CGACTGGAGA GGGTCACCAT TGCGGATGAA
    GGCGAGTACA GTTGCGAGGC GGACAATGTT GTGGGCGCCA TCACCGCGAT GGGAACTTTG
    ACTGTTTACG CCCCCCCGAA ATTCATCCAA CGTCCTGCGA GCAAATCCGT GGAACTCGGC
    GCCGACACAT CGTTCGAGTG TCGAGCTATA GGAAACCCAA AGCCCACCAT CTTCTGGACC
    ATCAAGAACA ACAGCACGCT GATATTTCCC GGAGCCCCGC CCTTGGATCG CTTTCATAGC
    CTCAACACTG AGGAAGGTCA CTCGATACTC ACGTTGACCA GGTTCCAGAG AACGGACAAG
    GATCTGGTCA TCCTGTGCAA CGCCATGAAC GAGGTGGCCA GCATTACCTC AAGGGTGCAG
    CTAAGCCTGG ACTCTCAAGA GGATCGACCA CCGCCGATCA TAATCTCCGG TCCGGTTAAT
    CAAACCCTAC CCATCAAGTC CCTGGCCACA CTTCAGTGCA AGGCCATCGG TTTGCCCAGT
    CCCACTATTT CCTGGTATCG CGATGGAATA CCAGTGCAAC CCAGTTCCAA GCTGAACATT
    ACCACATCCG GGGACTTGAT AATATCGGAC CTCGATCGAC AACAGGATCA AGGCCTATAC
    ACCTGCGTGG CCAGCTCGCG GGCTGGAAAG TCCACTTGGA GTGGTTTCCT CCGGATAGAG
    TTGCCAACCA ATCCGAATAT TAAGTTCTAC AGAGCCCCGG AGCAGACTAA GTGTCCCAGT
    GCTCCAGGGC AACCGAAAAT TCTGAACGCC ACTGCCTCGG CACTGACCAT TGTTTGGCCA
    ACCAGTGACA AGGCGGGAGC ATCGTCATTC CTAGGGTACA GTGTGGAAAT GTACTGCACC
    AATCAGAGCA GGACTTGGAT ACCCATTGCA TCGCGTTTGA GTGAGCCGAT TTTCACGGTC
    GAGAGTTTGA CGCAGGGAGC GGCTTACATG TTCATTGTTC GAGCGGAAAA CTCACTGGGC
    TTCTCGCCAC CCTCCCCCAT ATCGGAACCC ATTACGGCGG GAAAACTTGT GGGCGTGCGG
    GATGGTAGTG AAAGCACCGG GACCTCACAG CTCCTCCTCA GCGATGTGGA GACCTTGCTG
    CAGGCCAACG ATGTTGTGGA GCTACTGGAG GCCAATGCCA GTGATTCGAC GACCGCTCGA
    CTGTCTTGGG ACATAGATAG TGGTCAGTAC ATCGAGGGCT TCTATCTGTA CGCCCGGGAA
    CTGCACTCCT CCGAGTACAA AATGGTTACA CTGCTCAACA AGGGTCAGGG GCTGAGCTCC
    TGTACGGTGC CTGGACTGGC GAAGGCCTCC ACATACGAGT TTTTTCTTGT GCCATTTTAC
    AAGAGCATCG TAGGCAAGCC CTCGAACTCG CGACGCATGA GAACTTTGGA AGATGTTCCG
    GAGGCACCAC CTTATGGCAT GGAAGCCATT CAATTCAATC GCACCTCGGT CTTCTTGAAG
    TGGTTACCTC CACAACCAAA TCGGACTCGC AACGGCATCC TCACCAGCTA CAATGTGGTC
    GTCAAAGGCC TGGACGTGCA CAATACGACG CGGATATTCA AAAATATGAC CATAGACGCG
    GCCGCGCCCA CCCTGCTCCT GGCCAACCTC ACCACAGGAG TCACCTACTA TATCGCAGTG
    GCGGCTGCCA CGCGGGTGGG AGTCGGACCC TTCAGCAAGC CTGCCGTGCT CCGCATCGAT
    GCACGCACCC AATCCCTGGA CACTGGCTAC ACGAGATACC CCATCAGTCG TGATATTGCC
    GATGACTTTT TAACGCAGAC CTGGTTTATC GTCCTGCTGG GCTCCATCAT TGCCATAATT
    GTGTTTCTAT TGGGCGCATT GGTTCTATTC AAGCGCTACC AATTTATCAA GCAGACCTCG
    CTGGGCAGTT TACATGGCAA TCACGCAATC GGAACCGTGC GAAAGTTTCC AACATTGCCG
    CTAAATGGAG GCGGAGCCGT CGGTGCCGGT GGCTCAAATT CGACAGCCGC AGCTGGGCAG
    AGTGGCCATG GCCTGTGGAT CGATCCCACC AGCGGCGTTT GGCGACAGGC AGCGGGTGGC
    GGCAATCCTG GCGGAGTGGG CTCGTGTACG ACAAAGGAGC AACTTCCGGG ATACGCACAG
    GCCACCGCCC AACAAGGACA ACAGCCCACG CTGCTACCCG ATTACGAGAG ACTATCACCG
    CTGAATATGC CCGATTACGC GGAAGTCGCC TGCTCGACAT TCAAAAGCCC CACACACGGA
    GCACCGCCGA TGGGTGGTGG TGGTGGTGGC CAGTCGGCCT CCCTGTACGA CAGCTGTGGG
    GCCTACGCTA CCACCAATGT GGTGGCCAAT GTAAAGCTCT ACCAGAACCG CTATGCGACA
    AAGCCGAACC AGAGCAGCGG CAGCAATAAC AACAATCACC AGGGCAATGA CTACCAGTCG
    ACCGGGATGT ACTCCGCTCC ACCGAGCGCC CACTACGGAT GCTTGGAGCC AAAGCAGCAG
    CAGCAGCAGC AGCAGCCCAA TCTGATGACC ACCTCCACGG CCAGCACGGC CATCTTGACG
    AACTCGCCGG CCAAGGTGAA GAAGATCAAC ATAACCGAGA ACAAAATGGA TCAGCTGGAG
    GGCAAGTCGG AGCGGACGAA CCCGTTCAAC CAGCAGCAGC AGCTCCTCCT GGCCAGCAAC
    GCCCTGAAGC AGGGACTGGG CGCCTATGCC AACACCACCT TGGCGGCTCA GATGGCCAGT
    GGAGGTGGAG CGGGCACCTT GAGGCGCCAG CGTCAGCCGA AGACGCTTTA CAAAAGCGAA
    AACAATATAC TGGGCAAGTC TGGTCTGCGG CAGAACACCA TGAATGCGAG TGTAGGTGCT
    CCCAGCAACG GGCAGATGGA CTTCCTGACC GGCGGTCCTC CGTCGGAGGG CGGAGACTTC
    TCCGGACTGG GCCTCTGCAA CTCCACCAAC CAGCTGCTTA ACGACTGGGC ATCCAGTGCC
    TCGATCGCAG CTCCAGGGGA TTATCATTTC GGCAGCAAGC AGCCCAGCAA GCAGCACCTC
    TACGTGAAGG CCAAGGATGG CACCTGGTCG GCCGTCAGTT CGGATGCCTA TCAGTCCTTC
    AAGCATCAGC AGCAGCACCA TCCCTTCCTG GCAGGATCAG GTGACAACAC CAAGTCCCTA
    GCTAGTGTAA ACAGCTTAGC TGGCGATAGC AAATTCCTGA GTAGTTTTGG CTCTAGTGCC
    AATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_608592.2
    CDS475..4503
    Translation

    Target ORF information:

    RefSeq Version NM_134748.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster roundabout 3 (robo3), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_134748.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTAC GTTTCGTATT CATCGTTTTC CTGCTCAAGT GGACCCATGC ACAAGGATCC 
    CATCCGCCGC GGATAGTGGA GCATCCCATA GATACGACAG TGCCGCGACA CGAGCCAGCC
    ACGCTTAATT GCAAAGCCGA GGGCAGCCCC ACGCCCACCA TCCAGTGGTA CAAGGATGGG
    GTGCCCCTGA AGATCCTGCC GGGCTCCCAC CGCATCACCC TGCCAGCCGG CGGATTGTTT
    TTCCTCAAGG TTGTCAATTC ACGTCGCGAA ACAGATGCGG GCATCTACTG GTGCGAGGCG
    AAGAACGAAC TGGGCGTGGC AAGGAGTCGG AACGCCACGT TGCAAGTGGC GGTTCTCCGC
    GATGAGTTCC GCCTGGAGCC GCAGAACACA CGGATCGCCC AGGGCGATAC CGCCCTCCTC
    GAGTGCGCCG CCCCTCGGGG AATCCCCGAG CCCACAGTCA CCTGGAAGAA GGGCGGCCAG
    AAATTGGATT TGGAGGGCTC GAAGCGAGTA CGCATCGTTG ACGGCGGCAA TTTGGCCATC
    CAGGATGCCC GACAGACTGA CGAGGGTCAG TACCAGTGCA TAGCCAAGAA TCCTGTCGGG
    GTGCGCGAGT CCTCGTTGGC TACACTCAAA GTGCACGTCA AGCCGTACAT CATCCGGGGT
    CCGCACGATC AAACGGTTTT GGAGGGCGCC TCGGTGACCT TTCCCTGTCG AGTGGGTGGT
    GACCCCATGC CGGACGTACT CTGGCTGCGA ACCGCCTCGG GTGGCAACAT GCCATTGGAT
    CGCGTGAGTG TCCTGGAGGA TCGGAGTCTG CGACTGGAGA GGGTCACCAT TGCGGATGAA
    GGCGAGTACA GTTGCGAGGC GGACAATGTT GTGGGCGCCA TCACCGCGAT GGGAACTTTG
    ACTGTTTACG CCCCCCCGAA ATTCATCCAA CGTCCTGCGA GCAAATCCGT GGAACTCGGC
    GCCGACACAT CGTTCGAGTG TCGAGCTATA GGAAACCCAA AGCCCACCAT CTTCTGGACC
    ATCAAGAACA ACAGCACGCT GATATTTCCC GGAGCCCCGC CCTTGGATCG CTTTCATAGC
    CTCAACACTG AGGAAGGTCA CTCGATACTC ACGTTGACCA GGTTCCAGAG AACGGACAAG
    GATCTGGTCA TCCTGTGCAA CGCCATGAAC GAGGTGGCCA GCATTACCTC AAGGGTGCAG
    CTAAGCCTGG ACTCTCAAGA GGATCGACCA CCGCCGATCA TAATCTCCGG TCCGGTTAAT
    CAAACCCTAC CCATCAAGTC CCTGGCCACA CTTCAGTGCA AGGCCATCGG TTTGCCCAGT
    CCCACTATTT CCTGGTATCG CGATGGAATA CCAGTGCAAC CCAGTTCCAA GCTGAACATT
    ACCACATCCG GGGACTTGAT AATATCGGAC CTCGATCGAC AACAGGATCA AGGCCTATAC
    ACCTGCGTGG CCAGCTCGCG GGCTGGAAAG TCCACTTGGA GTGGTTTCCT CCGGATAGAG
    TTGCCAACCA ATCCGAATAT TAAGTTCTAC AGAGCCCCGG AGCAGACTAA GTGTCCCAGT
    GCTCCAGGGC AACCGAAAAT TCTGAACGCC ACTGCCTCGG CACTGACCAT TGTTTGGCCA
    ACCAGTGACA AGGCGGGAGC ATCGTCATTC CTAGGGTACA GTGTGGAAAT GTACTGCACC
    AATCAGAGCA GGACTTGGAT ACCCATTGCA TCGCGTTTGA GTGAGCCGAT TTTCACGGTC
    GAGAGTTTGA CGCAGGGAGC GGCTTACATG TTCATTGTTC GAGCGGAAAA CTCACTGGGC
    TTCTCGCCAC CCTCCCCCAT ATCGGAACCC ATTACGGCGG GAAAACTTGT GGGCGTGCGG
    GATGGTAGTG AAAGCACCGG GACCTCACAG CTCCTCCTCA GCGATGTGGA GACCTTGCTG
    CAGGCCAACG ATGTTGTGGA GCTACTGGAG GCCAATGCCA GTGATTCGAC GACCGCTCGA
    CTGTCTTGGG ACATAGATAG TGGTCAGTAC ATCGAGGGCT TCTATCTGTA CGCCCGGGAA
    CTGCACTCCT CCGAGTACAA AATGGTTACA CTGCTCAACA AGGGTCAGGG GCTGAGCTCC
    TGTACGGTGC CTGGACTGGC GAAGGCCTCC ACATACGAGT TTTTTCTTGT GCCATTTTAC
    AAGAGCATCG TAGGCAAGCC CTCGAACTCG CGACGCATGA GAACTTTGGA AGATGTTCCG
    GAGGCACCAC CTTATGGCAT GGAAGCCATT CAATTCAATC GCACCTCGGT CTTCTTGAAG
    TGGTTACCTC CACAACCAAA TCGGACTCGC AACGGCATCC TCACCAGCTA CAATGTGGTC
    GTCAAAGGCC TGGACGTGCA CAATACGACG CGGATATTCA AAAATATGAC CATAGACGCG
    GCCGCGCCCA CCCTGCTCCT GGCCAACCTC ACCACAGGAG TCACCTACTA TATCGCAGTG
    GCGGCTGCCA CGCGGGTGGG AGTCGGACCC TTCAGCAAGC CTGCCGTGCT CCGCATCGAT
    GCACGCACCC AATCCCTGGA CACTGGCTAC ACGAGATACC CCATCAGTCG TGATATTGCC
    GATGACTTTT TAACGCAGAC CTGGTTTATC GTCCTGCTGG GCTCCATCAT TGCCATAATT
    GTGTTTCTAT TGGGCGCATT GGTTCTATTC AAGCGCTACC AATTTATCAA GCAGACCTCG
    CTGGGCAGTT TACATGGCAA TCACGCAATC GGAACCGTGC GAAAGTTTCC AACATTGCCG
    CTAAATGGAG GCGGAGCCGT CGGTGCCGGT GGCTCAAATT CGACAGCCGC AGCTGGGCAG
    AGTGGCCATG GCCTGTGGAT CGATCCCACC AGCGGCGTTT GGCGACAGGC AGCGGGTGGC
    GGCAATCCTG GCGGAGTGGG CTCGTGTACG ACAAAGGAGC AACTTCCGGG ATACGCACAG
    GCCACCGCCC AACAAGGACA ACAGCCCACG CTGCTACCCG ATTACGAGAG ACTATCACCG
    CTGAATATGC CCGATTACGC GGAAGTCGCC TGCTCGACAT TCAAAAGCCC CACACACGGA
    GCACCGCCGA TGGGTGGTGG TGGTGGTGGC CAGTCGGCCT CCCTGTACGA CAGCTGTGGG
    GCCTACGCTA CCACCAATGT GGTGGCCAAT GTAAAGCTCT ACCAGAACCG CTATGCGACA
    AAGCCGAACC AGAGCAGCGG CAGCAATAAC AACAATCACC AGGGCAATGA CTACCAGTCG
    ACCGGGATGT ACTCCGCTCC ACCGAGCGCC CACTACGGAT GCTTGGAGCC AAAGCAGCAG
    CAGCAGCAGC AGCAGCCCAA TCTGATGACC ACCTCCACGG CCAGCACGGC CATCTTGACG
    AACTCGCCGG CCAAGGTGAA GAAGATCAAC ATAACCGAGA ACAAAATGGA TCAGCTGGAG
    GGCAAGTCGG AGCGGACGAA CCCGTTCAAC CAGCAGCAGC AGCTCCTCCT GGCCAGCAAC
    GCCCTGAAGC AGGGACTGGG CGCCTATGCC AACACCACCT TGGCGGCTCA GATGGCCAGT
    GGAGGTGGAG CGGGCACCTT GAGGCGCCAG CGTCAGCCGA AGACGCTTTA CAAAAGCGAA
    AACAATATAC TGGGCAAGTC TGGTCTGCGG CAGAACACCA TGAATGCGAG TGTAGGTGCT
    CCCAGCAACG GGCAGATGGA CTTCCTGACC GGCGGTCCTC CGTCGGAGGG CGGAGACTTC
    TCCGGACTGG GCCTCTGCAA CTCCACCAAC CAGCTGCTTA ACGACTGGGC ATCCAGTGCC
    TCGATCGCAG CTCCAGGGGA TTATCATTTC GGCAGCAAGC AGCCCAGCAA GCAGCACCTC
    TACGTGAAGG CCAAGGATGG CACCTGGTCG GCCGTCAGTT CGGATGCCTA TCAGTCCTTC
    AAGCATCAGC AGCAGCACCA TCCCTTCCTG GCAGGATCAG GTGACAACAC CAAGTCCCTA
    GCTAGTGTAA ACAGCTTAGC TGGCGATAGC AAATTCCTGA GTAGTTTTGG CTCTAGTGCC
    AATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.