ROBO1 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_016941507.1	XP_016796996.1	roundabout homolog 1 isoform X12
XM_024355570.1	XP_024211338.1	roundabout homolog 1 isoform X5
XM_024355571.1	XP_024211339.1	roundabout homolog 1 isoform X6
XM_024355573.1	XP_024211341.1	roundabout homolog 1 isoform X11
XM_016941504.2	XP_016796993.1	roundabout homolog 1 isoform X13
XM_003309895.4	XP_003309943.2	roundabout homolog 1 isoform X15
XM_016941508.2	XP_016796997.1	roundabout homolog 1 isoform X16
XM_016941496.1	XP_016796985.1	roundabout homolog 1 isoform X1
XM_016941498.1	XP_016796987.1	roundabout homolog 1 isoform X3
XM_016941504.1	XP_016796993.1	roundabout homolog 1 isoform X9
XM_024355567.1	XP_024211335.1	roundabout homolog 1 isoform X2
XM_016941499.1	XP_016796988.1	roundabout homolog 1 isoform X4
XM_016941501.1	XP_016796990.1	roundabout homolog 1 isoform X6
XM_016941502.1	XP_016796991.1	roundabout homolog 1 isoform X7
XM_016941503.1	XP_016796992.1	roundabout homolog 1 isoform X8
XM_516592.5	XP_516592.4	roundabout homolog 1 isoform X10
XM_016941508.1	XP_016796997.1	roundabout homolog 1 isoform X14
XM_016941496.2	XP_016796985.1	roundabout homolog 1 isoform X7
XM_016941499.2	XP_016796988.1	roundabout homolog 1 isoform X8
XM_016941502.2	XP_016796991.1	roundabout homolog 1 isoform X9
XM_016941503.2	XP_016796992.1	roundabout homolog 1 isoform X12
XM_516592.6	XP_516592.4	roundabout homolog 1 isoform X14
XM_016941497.1	XP_016796986.1	roundabout homolog 1 isoform X2
XM_016941500.1	XP_016796989.1	roundabout homolog 1 isoform X5
XM_016941506.1	XP_016796995.1	roundabout homolog 1 isoform X11
XM_003309895.3	XP_003309943.2	roundabout homolog 1 isoform X13
XM_024355566.1	XP_024211334.1	roundabout homolog 1 isoform X1
XM_024355568.1	XP_024211336.1	roundabout homolog 1 isoform X3
XM_024355569.1	XP_024211337.1	roundabout homolog 1 isoform X4
XM_024355572.1	XP_024211340.1	roundabout homolog 1 isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc105009	XM_024355566.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc105010	XM_024355567.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc105011	XM_024355568.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc105012	XM_024355569.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc105013	XM_024355570.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc105014	XM_024355571.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc105015	XM_016941496.2 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc105016	XM_016941499.2 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc105017	XM_016941502.2 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc105018	XM_024355572.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc105019	XM_024355573.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc105020	XM_016941503.2 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc105021	XM_016941504.2 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc105022	XM_516592.6 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc105023	XM_003309895.4 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc105024	XM_016941508.2 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc82301	XM_016941496.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82302	XM_016941497.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82303	XM_016941498.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82304	XM_016941499.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82305	XM_016941500.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82306	XM_016941501.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82307	XM_016941502.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82308	XM_016941503.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82309	XM_016941504.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82310	XM_516592.5 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82311	XM_016941506.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82312	XM_016941507.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82313	XM_003309895.3 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc82314	XM_016941508.1 Latest version!	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ROBO1 ( XM_024355566.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211334.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc105009
Clone ID Related Accession (Same CDS sequence)	XM_024355566.1
Accession Version	XM_024355566.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TCCCGTCTTT TACCTTCACA 
CCAACAGTAA CTTACCAGAG AGGAGGCGAA GCTGTCAGCA GTGGAGGGAG GCCCGGACTT 
CTCAACATCA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC TAATACTGGC 
AACAACCACA ATGACTGCTC CATCAGCTGC TGCACGGCAG GCAATGGAAA CAGCGACAGC 
AACCTCACTA CCTACAGTCG CCCAGCTGAT TGTATAGCAA ATTATAACAA CCAACTGGAT 
AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT 
AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGATGG GCGTTTTGTC 
AATCCATCAG GGCAGCCTAC TCCTTATGCC ACCACTCAGC TCATCCAGTC AAACCTCAGC 
AACAACATGA ACAATGGCAG CGGGGACTCT GGCGAGAAGC ACTGGAAACC ACTGGGACAG 
CAGAAACAAG AAGTGGCACC AGTTCAGTAC AACATCATGG AGCAAAACAA GCTGAACAAA 
GGTGAAGAGG CTTATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211334.1
CDS	55..5130
Translation	MMLRPSQGLVCRRRGKDEACCVLSLCVFLSHVDLLVSGHAVIEMATTVTTVTPSAALVLFPTDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_024355566.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024355566.1

ORF Insert Sequence:

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TCCCGTCTTT TACCTTCACA 
CCAACAGTAA CTTACCAGAG AGGAGGCGAA GCTGTCAGCA GTGGAGGGAG GCCCGGACTT 
CTCAACATCA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC TAATACTGGC 
AACAACCACA ATGACTGCTC CATCAGCTGC TGCACGGCAG GCAATGGAAA CAGCGACAGC 
AACCTCACTA CCTACAGTCG CCCAGCTGAT TGTATAGCAA ATTATAACAA CCAACTGGAT 
AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT 
AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGATGG GCGTTTTGTC 
AATCCATCAG GGCAGCCTAC TCCTTATGCC ACCACTCAGC TCATCCAGTC AAACCTCAGC 
AACAACATGA ACAATGGCAG CGGGGACTCT GGCGAGAAGC ACTGGAAACC ACTGGGACAG 
CAGAAACAAG AAGTGGCACC AGTTCAGTAC AACATCATGG AGCAAAACAA GCTGAACAAA 
GGTGAAGAGG CTTATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_024355567.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211335.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

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CloneID	OPc105010
Clone ID Related Accession (Same CDS sequence)	XM_024355567.1
Accession Version	XM_024355567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGAACCTC CACATTTTGT TGTGAAACCC CGTGACCAGG TTGTTGCTTT GGGACGGACT 
GTAACTTTTC AGTGTGAAGC AACCGGAAAT CCTCAACCAG CTATTTTTTG GAGGAGAGAA 
GGGAGTCAGA ATCTACTTTT CTCATATCAA CCACCACAGT CATCCAGCCG ATTTTCAGTC 
TCCCAGACTG GCGACCTCAC AATTACTAAT GTCCAGCGAT CTGATGTTGG TTATTACATC 
TGCCAGACTT TAAATGTTGC TGGAAGCATC ATCACAAAGG CATATTTGGA AGTTACAGAT 
GTGATTGCAG ATCGGCCTCC CCCAGTTATT CGACAAGGTC CTGTGAATCA GACTGTAGCT 
GTGGATGGCA CTTTCGTCCT CAGCTGTGTG GCCACAGGCA GTCCAGTGCC CACCATTCTG 
TGGAGAAAGG ATGGAGTCCT CGTTTCAACC CAAGACTCTC GAATCAAACA GTTGGAGAAT 
GGAGTACTGC AGATCCGATA TGCTAAGCTG GGTGATACTG GTCGGTACAC CTGCATTGCA 
TCAACCCCCA GTGGTGAAGC AACATGGAGT GCTTACATTG AAGTTCAAGA ATTTGGAGTT 
CCAGTTCAGC CTCCAAGACC TACTGACCCA AATTTAATCC CTAGTGCCCC ATCAAAACCT 
GAAGTGACAG ATGTCAGCAG AAATACAGTC ACATTATCAT GGCAACCAAA TTTGAATTCA 
GGAGCAACTC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG 
CAGACCGTAG CAGAGAATGT GAAAACAGAA ACATTTGCCA TTAAAGGACT CAAACCTAAT 
GCAATTTACC TTTTCCTTGT GAGGGCAGCT AATGCATATG GAATTAGTGA TCCAAGCCAA 
ATATCAGATC CAGTGAAAAC ACAAGATGTC CTACCAACAA GTCAGGGGGT GGACCACAAG 
CAGGTCCAGA GAGAGCTGGG GAATGCTGTT CTGCACCTCC ACAACCCCAC CGTCCTTTCT 
TCCTCTTCCA TCGAAGTGCA CTGGACAGTA GATCAACAGT CTCAGTATAT ACAAGGATAT 
AAAATTCTCT ATCGGCCATC TGGAGCCAAC CACGGAGAAT CAGACTGGTT AGTTTTTGAA 
GTGAGGACGC CAGCCAAAAA CAGTGTGGTT ATCCCTGATC TCAGAAAGGG AGTCAACTAT 
GAAATTAAGG CTCGCCCTTT TTTTAATGAA TTTCAAGGAG CAGATAGTGA AATCAAGTTC 
GCCAAAACCC TGGAAGAAGC ACCCAGTGCC CCACCCCAAG GTGTAACTGT ATCCAAGAAT 
GATGGAAACG GAACTGCAAT TCTAGTTAGT TGGCAGCCAC CTCCAGAAGA CACTCAAAAT 
GGAATGGTCC AAGAGTATAA GGTTTGGTGT CTGGGCAATG AAACTCGATA CCACATCAAC 
AAAACAGTGG ATGGTTCCAC CTTTTCCGTG GTCATTCCCT TTCTTGTTCC TGGAATCCGA 
TACAGTGTGG AAGTGGCAGC CAGCACTGGG GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG 
TTCATCCAGC TGGATGCCCA TGGAAACCCT GTGTCACCTG AGGACCAAGT CAGCCTCGCT 
CAGCAGATTT CAGATGTGGT GAAGCAGCCG GCCTTCATAG CAGGTATTGG AGCAGCCTGT 
TGGATCATCC TCATGGTCTT CAGCATCTGG CTTTATCGAC ACCGCAAGAA GAGAAACGGA 
CTTACTAGTA CTTACGCGGG TATCAGAAAA GTCCCGTCTT TTACCTTCAC ACCAACAGTA 
ACTTACCAGA GAGGAGGCGA AGCTGTCAGC AGTGGAGGGA GGCCCGGACT TCTCAACATC 
AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CTAATACTGG CAACAACCAC 
AATGACTGCT CCATCAGCTG CTGCACGGCA GGCAATGGAA ACAGCGACAG CAACCTCACT 
ACCTACAGTC GCCCAGCTGA TTGTATAGCA AATTATAACA ACCAACTGGA TAACAAACAA 
ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC 
AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGATG GGCGTTTTGT CAATCCATCA 
GGGCAGCCTA CTCCTTATGC CACCACTCAG CTCATCCAGT CAAACCTCAG CAACAACATG 
AACAATGGCA GCGGGGACTC TGGCGAGAAG CACTGGAAAC CACTGGGACA GCAGAAACAA 
GAAGTGGCAC CAGTTCAGTA CAACATCATG GAGCAAAACA AGCTGAACAA AGGTGAAGAG 
GCTTATTATC GAGCAAATGA CACAGTTCCT CAAACTATCC CATACAACCA ATCATACGAC 
CAGAACACAG GAGGATCCTA CAACAGCTCA GACCGGGGCA GTAGTACATC TGGGAGTCAG 
GGGCACAAGA AAGGGGCAAG AACACCCAAG GTACCAAAAC AGGGTGGCAT GAACTGGGCA 
GACCTGCTTC CTCCTCCCCC AGCACATCCT CCTCCACACA GCAATAGCGA AGAGTACAAC 
ATTTCTGTAG ATGAAAGCTA TGACCAAGAA ATGCCATGTC CCGTGCCACC AGCAAGGATG 
TATTTGCAAC AAGATGAATT AGAAGAGGAG GAAGATGAAC GAGGCCCCAC TCCCCCCGTT 
CGGGGAGCAG CTTCTTCTCC AGCTGCCGTG TCCTATAGCC ATCAGTCCAC TGCCACTCTG 
ACTCCCTCCC CACAGGAAGA ACTCCAGCCC ATGTTACAGG ATTGTCCAGA GGAGACTGGC 
CACATGCAGC ACCAGCCCGA CAGGAGACGG CAGCCTGTGA GTCCTCCTCC ACCACCACGG 
CCGATCTCCC CTCCACATAC CTATGGCTAC ATTTCAGGAC CCCTGGTCTC AGATATGGAT 
ACAGATGCGC CAGAAGAGGA AGAAGACGAA GCCGACATGG AGGTAGCCAA GATGCAAACC 
AGAAGGCTTT TGTTACGTGG GCTTGAGCAG ACACCTGCCT CCAGTGTTGG GGACCTGGAG 
AGCTCTGTCA CGGGGTCCAT GATCAACGGC TGGGGCTCAG CCTCAGAGGA GGACAACATT 
TCCAGCGGAC GCTCCAGTGT TAGTTCTTCG GATGGCTCCT TTTTCACTGA TGCTGACTTT 
GCCCAGGCAG TCGCAGCAGC GGCAGAGTAT GCTGGTCTGA AAGTGGCACG ACGGCAAATG 
CAGGATGCTG CTGGCCGTCG ACATTTTCAT GCGTCTCAGT GCCCTAGGCC CACAAGTCCT 
GTGTCTACAG ACAGCAACAT GAGTGCCGCC GTAATGCAGA AAACCAGACC AGCCAAGAAA 
CCGAAACACC AGCCAGGACA TCTGCGCAGA GAAACCTACA CAGATGATCT TCCACCACCT 
CCTGTGCCGC CACCTGCTAT AAAGTCACCT ACTGCCCAAT CCAAGACACA GCTGGAAGTA 
CGACCTGTAG TGGTGCCAAA ACTCCCTTCT GTGGATGCAA GAACAGACAG ATCATCAGAC 
AGAAAAGGAA GCAGTTACAA GGGGAGAGAA GTATTGGATG GAAGACAGGT TGTTGACATG 
CGAACAAATC CAGGTGATCC CAGAGAAGCA CAGGAACAGC AAAATGACGG GAAAGGACGT 
GGAAACAAGG CAGCAAAACG AGACCTTCCA CCAGCAAAGA CTCATCTCAT CCAAGAGGAT 
ATTCTACCTT ATTGTAGACC TACTTTTCCA ACATCAAATA ATCCCAGAGA TCCCAGTTCC 
TCAAGCTCAA TGTCATCAAG AGGATCAGGA AGCAGACAAA GAGAACAAGC AAATGTAGGT 
CGAAGAAATA TTGCAGAAAT GCAGGTACTT GGAGGATATG AAAGAGGAGA AGATAATAAT 
GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211335.1
CDS	56..5122
Translation	MMLRPSQGLVCRRRGKDEACCVLSLCVFLSHVDLLVSGHAVIEMATTVTTVTPSAALVLFPTDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_024355567.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024355567.1

ORF Insert Sequence:

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGAACCTC CACATTTTGT TGTGAAACCC CGTGACCAGG TTGTTGCTTT GGGACGGACT 
GTAACTTTTC AGTGTGAAGC AACCGGAAAT CCTCAACCAG CTATTTTTTG GAGGAGAGAA 
GGGAGTCAGA ATCTACTTTT CTCATATCAA CCACCACAGT CATCCAGCCG ATTTTCAGTC 
TCCCAGACTG GCGACCTCAC AATTACTAAT GTCCAGCGAT CTGATGTTGG TTATTACATC 
TGCCAGACTT TAAATGTTGC TGGAAGCATC ATCACAAAGG CATATTTGGA AGTTACAGAT 
GTGATTGCAG ATCGGCCTCC CCCAGTTATT CGACAAGGTC CTGTGAATCA GACTGTAGCT 
GTGGATGGCA CTTTCGTCCT CAGCTGTGTG GCCACAGGCA GTCCAGTGCC CACCATTCTG 
TGGAGAAAGG ATGGAGTCCT CGTTTCAACC CAAGACTCTC GAATCAAACA GTTGGAGAAT 
GGAGTACTGC AGATCCGATA TGCTAAGCTG GGTGATACTG GTCGGTACAC CTGCATTGCA 
TCAACCCCCA GTGGTGAAGC AACATGGAGT GCTTACATTG AAGTTCAAGA ATTTGGAGTT 
CCAGTTCAGC CTCCAAGACC TACTGACCCA AATTTAATCC CTAGTGCCCC ATCAAAACCT 
GAAGTGACAG ATGTCAGCAG AAATACAGTC ACATTATCAT GGCAACCAAA TTTGAATTCA 
GGAGCAACTC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG 
CAGACCGTAG CAGAGAATGT GAAAACAGAA ACATTTGCCA TTAAAGGACT CAAACCTAAT 
GCAATTTACC TTTTCCTTGT GAGGGCAGCT AATGCATATG GAATTAGTGA TCCAAGCCAA 
ATATCAGATC CAGTGAAAAC ACAAGATGTC CTACCAACAA GTCAGGGGGT GGACCACAAG 
CAGGTCCAGA GAGAGCTGGG GAATGCTGTT CTGCACCTCC ACAACCCCAC CGTCCTTTCT 
TCCTCTTCCA TCGAAGTGCA CTGGACAGTA GATCAACAGT CTCAGTATAT ACAAGGATAT 
AAAATTCTCT ATCGGCCATC TGGAGCCAAC CACGGAGAAT CAGACTGGTT AGTTTTTGAA 
GTGAGGACGC CAGCCAAAAA CAGTGTGGTT ATCCCTGATC TCAGAAAGGG AGTCAACTAT 
GAAATTAAGG CTCGCCCTTT TTTTAATGAA TTTCAAGGAG CAGATAGTGA AATCAAGTTC 
GCCAAAACCC TGGAAGAAGC ACCCAGTGCC CCACCCCAAG GTGTAACTGT ATCCAAGAAT 
GATGGAAACG GAACTGCAAT TCTAGTTAGT TGGCAGCCAC CTCCAGAAGA CACTCAAAAT 
GGAATGGTCC AAGAGTATAA GGTTTGGTGT CTGGGCAATG AAACTCGATA CCACATCAAC 
AAAACAGTGG ATGGTTCCAC CTTTTCCGTG GTCATTCCCT TTCTTGTTCC TGGAATCCGA 
TACAGTGTGG AAGTGGCAGC CAGCACTGGG GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG 
TTCATCCAGC TGGATGCCCA TGGAAACCCT GTGTCACCTG AGGACCAAGT CAGCCTCGCT 
CAGCAGATTT CAGATGTGGT GAAGCAGCCG GCCTTCATAG CAGGTATTGG AGCAGCCTGT 
TGGATCATCC TCATGGTCTT CAGCATCTGG CTTTATCGAC ACCGCAAGAA GAGAAACGGA 
CTTACTAGTA CTTACGCGGG TATCAGAAAA GTCCCGTCTT TTACCTTCAC ACCAACAGTA 
ACTTACCAGA GAGGAGGCGA AGCTGTCAGC AGTGGAGGGA GGCCCGGACT TCTCAACATC 
AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CTAATACTGG CAACAACCAC 
AATGACTGCT CCATCAGCTG CTGCACGGCA GGCAATGGAA ACAGCGACAG CAACCTCACT 
ACCTACAGTC GCCCAGCTGA TTGTATAGCA AATTATAACA ACCAACTGGA TAACAAACAA 
ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC 
AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGATG GGCGTTTTGT CAATCCATCA 
GGGCAGCCTA CTCCTTATGC CACCACTCAG CTCATCCAGT CAAACCTCAG CAACAACATG 
AACAATGGCA GCGGGGACTC TGGCGAGAAG CACTGGAAAC CACTGGGACA GCAGAAACAA 
GAAGTGGCAC CAGTTCAGTA CAACATCATG GAGCAAAACA AGCTGAACAA AGGTGAAGAG 
GCTTATTATC GAGCAAATGA CACAGTTCCT CAAACTATCC CATACAACCA ATCATACGAC 
CAGAACACAG GAGGATCCTA CAACAGCTCA GACCGGGGCA GTAGTACATC TGGGAGTCAG 
GGGCACAAGA AAGGGGCAAG AACACCCAAG GTACCAAAAC AGGGTGGCAT GAACTGGGCA 
GACCTGCTTC CTCCTCCCCC AGCACATCCT CCTCCACACA GCAATAGCGA AGAGTACAAC 
ATTTCTGTAG ATGAAAGCTA TGACCAAGAA ATGCCATGTC CCGTGCCACC AGCAAGGATG 
TATTTGCAAC AAGATGAATT AGAAGAGGAG GAAGATGAAC GAGGCCCCAC TCCCCCCGTT 
CGGGGAGCAG CTTCTTCTCC AGCTGCCGTG TCCTATAGCC ATCAGTCCAC TGCCACTCTG 
ACTCCCTCCC CACAGGAAGA ACTCCAGCCC ATGTTACAGG ATTGTCCAGA GGAGACTGGC 
CACATGCAGC ACCAGCCCGA CAGGAGACGG CAGCCTGTGA GTCCTCCTCC ACCACCACGG 
CCGATCTCCC CTCCACATAC CTATGGCTAC ATTTCAGGAC CCCTGGTCTC AGATATGGAT 
ACAGATGCGC CAGAAGAGGA AGAAGACGAA GCCGACATGG AGGTAGCCAA GATGCAAACC 
AGAAGGCTTT TGTTACGTGG GCTTGAGCAG ACACCTGCCT CCAGTGTTGG GGACCTGGAG 
AGCTCTGTCA CGGGGTCCAT GATCAACGGC TGGGGCTCAG CCTCAGAGGA GGACAACATT 
TCCAGCGGAC GCTCCAGTGT TAGTTCTTCG GATGGCTCCT TTTTCACTGA TGCTGACTTT 
GCCCAGGCAG TCGCAGCAGC GGCAGAGTAT GCTGGTCTGA AAGTGGCACG ACGGCAAATG 
CAGGATGCTG CTGGCCGTCG ACATTTTCAT GCGTCTCAGT GCCCTAGGCC CACAAGTCCT 
GTGTCTACAG ACAGCAACAT GAGTGCCGCC GTAATGCAGA AAACCAGACC AGCCAAGAAA 
CCGAAACACC AGCCAGGACA TCTGCGCAGA GAAACCTACA CAGATGATCT TCCACCACCT 
CCTGTGCCGC CACCTGCTAT AAAGTCACCT ACTGCCCAAT CCAAGACACA GCTGGAAGTA 
CGACCTGTAG TGGTGCCAAA ACTCCCTTCT GTGGATGCAA GAACAGACAG ATCATCAGAC 
AGAAAAGGAA GCAGTTACAA GGGGAGAGAA GTATTGGATG GAAGACAGGT TGTTGACATG 
CGAACAAATC CAGGTGATCC CAGAGAAGCA CAGGAACAGC AAAATGACGG GAAAGGACGT 
GGAAACAAGG CAGCAAAACG AGACCTTCCA CCAGCAAAGA CTCATCTCAT CCAAGAGGAT 
ATTCTACCTT ATTGTAGACC TACTTTTCCA ACATCAAATA ATCCCAGAGA TCCCAGTTCC 
TCAAGCTCAA TGTCATCAAG AGGATCAGGA AGCAGACAAA GAGAACAAGC AAATGTAGGT 
CGAAGAAATA TTGCAGAAAT GCAGGTACTT GGAGGATATG AAAGAGGAGA AGATAATAAT 
GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_024355568.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211336.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc105011
Clone ID Related Accession (Same CDS sequence)	XM_024355568.1
Accession Version	XM_024355568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TCCCGTCTTT TACCTTCACA 
CCAACAGTAA CTTACCAGAG AGGAGGCGAA GCTGTCAGCA GTGGAGGGAG GCCCGGACTT 
CTCAACATCA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC TAATACTGGC 
AACAACCACA ATGACTGCTC CATCAGCTGC TGCACGGCAG GCAATGGAAA CAGCGACAGC 
AACCTCACTA CCTACAGTCG CCCAGCTGAT TGTATAGCAA ATTATAACAA CCAACTGGAT 
AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT 
AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGATGG GCGTTTTGTC 
AATCCATCAG GGCAGCCTAC TCCTTATGCC ACCACTCAGC TCATCCAGTC AAACCTCAGC 
AACAACATGA ACAATGGCAG CGGGGACTCT GGCGAGAAGC ACTGGAAACC ACTGGGACAG 
CAGAAACAAG AAGTGGCACC AGTTCAGTAC AACATCATGG AGCAAAACAA GCTGAACAAA 
GATTATCGAG CAAATGACAC AGTTCCTCAA ACTATCCCAT ACAACCAATC ATACGACCAG 
AACACAGGAG GATCCTACAA CAGCTCAGAC CGGGGCAGTA GTACATCTGG GAGTCAGGGG 
CACAAGAAAG GGGCAAGAAC ACCCAAGGTA CCAAAACAGG GTGGCATGAA CTGGGCAGAC 
CTGCTTCCTC CTCCCCCAGC ACATCCTCCT CCACACAGCA ATAGCGAAGA GTACAACATT 
TCTGTAGATG AAAGCTATGA CCAAGAAATG CCATGTCCCG TGCCACCAGC AAGGATGTAT 
TTGCAACAAG ATGAATTAGA AGAGGAGGAA GATGAACGAG GCCCCACTCC CCCCGTTCGG 
GGAGCAGCTT CTTCTCCAGC TGCCGTGTCC TATAGCCATC AGTCCACTGC CACTCTGACT 
CCCTCCCCAC AGGAAGAACT CCAGCCCATG TTACAGGATT GTCCAGAGGA GACTGGCCAC 
ATGCAGCACC AGCCCGACAG GAGACGGCAG CCTGTGAGTC CTCCTCCACC ACCACGGCCG 
ATCTCCCCTC CACATACCTA TGGCTACATT TCAGGACCCC TGGTCTCAGA TATGGATACA 
GATGCGCCAG AAGAGGAAGA AGACGAAGCC GACATGGAGG TAGCCAAGAT GCAAACCAGA 
AGGCTTTTGT TACGTGGGCT TGAGCAGACA CCTGCCTCCA GTGTTGGGGA CCTGGAGAGC 
TCTGTCACGG GGTCCATGAT CAACGGCTGG GGCTCAGCCT CAGAGGAGGA CAACATTTCC 
AGCGGACGCT CCAGTGTTAG TTCTTCGGAT GGCTCCTTTT TCACTGATGC TGACTTTGCC 
CAGGCAGTCG CAGCAGCGGC AGAGTATGCT GGTCTGAAAG TGGCACGACG GCAAATGCAG 
GATGCTGCTG GCCGTCGACA TTTTCATGCG TCTCAGTGCC CTAGGCCCAC AAGTCCTGTG 
TCTACAGACA GCAACATGAG TGCCGCCGTA ATGCAGAAAA CCAGACCAGC CAAGAAACCG 
AAACACCAGC CAGGACATCT GCGCAGAGAA ACCTACACAG ATGATCTTCC ACCACCTCCT 
GTGCCGCCAC CTGCTATAAA GTCACCTACT GCCCAATCCA AGACACAGCT GGAAGTACGA 
CCTGTAGTGG TGCCAAAACT CCCTTCTGTG GATGCAAGAA CAGACAGATC ATCAGACAGA 
AAAGGAAGCA GTTACAAGGG GAGAGAAGTA TTGGATGGAA GACAGGTTGT TGACATGCGA 
ACAAATCCAG GTGATCCCAG AGAAGCACAG GAACAGCAAA ATGACGGGAA AGGACGTGGA 
AACAAGGCAG CAAAACGAGA CCTTCCACCA GCAAAGACTC ATCTCATCCA AGAGGATATT 
CTACCTTATT GTAGACCTAC TTTTCCAACA TCAAATAATC CCAGAGATCC CAGTTCCTCA 
AGCTCAATGT CATCAAGAGG ATCAGGAAGC AGACAAAGAG AACAAGCAAA TGTAGGTCGA 
AGAAATATTG CAGAAATGCA GGTACTTGGA GGATATGAAA GAGGAGAAGA TAATAATGAA 
GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211336.1
CDS	54..5117
Translation	MMLRPSQGLVCRRRGKDEACCVLSLCVFLSHVDLLVSGHAVIEMATTVTTVTPSAALVLFPTDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_024355568.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024355568.1

ORF Insert Sequence:

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TCCCGTCTTT TACCTTCACA 
CCAACAGTAA CTTACCAGAG AGGAGGCGAA GCTGTCAGCA GTGGAGGGAG GCCCGGACTT 
CTCAACATCA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC TAATACTGGC 
AACAACCACA ATGACTGCTC CATCAGCTGC TGCACGGCAG GCAATGGAAA CAGCGACAGC 
AACCTCACTA CCTACAGTCG CCCAGCTGAT TGTATAGCAA ATTATAACAA CCAACTGGAT 
AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT 
AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGATGG GCGTTTTGTC 
AATCCATCAG GGCAGCCTAC TCCTTATGCC ACCACTCAGC TCATCCAGTC AAACCTCAGC 
AACAACATGA ACAATGGCAG CGGGGACTCT GGCGAGAAGC ACTGGAAACC ACTGGGACAG 
CAGAAACAAG AAGTGGCACC AGTTCAGTAC AACATCATGG AGCAAAACAA GCTGAACAAA 
GATTATCGAG CAAATGACAC AGTTCCTCAA ACTATCCCAT ACAACCAATC ATACGACCAG 
AACACAGGAG GATCCTACAA CAGCTCAGAC CGGGGCAGTA GTACATCTGG GAGTCAGGGG 
CACAAGAAAG GGGCAAGAAC ACCCAAGGTA CCAAAACAGG GTGGCATGAA CTGGGCAGAC 
CTGCTTCCTC CTCCCCCAGC ACATCCTCCT CCACACAGCA ATAGCGAAGA GTACAACATT 
TCTGTAGATG AAAGCTATGA CCAAGAAATG CCATGTCCCG TGCCACCAGC AAGGATGTAT 
TTGCAACAAG ATGAATTAGA AGAGGAGGAA GATGAACGAG GCCCCACTCC CCCCGTTCGG 
GGAGCAGCTT CTTCTCCAGC TGCCGTGTCC TATAGCCATC AGTCCACTGC CACTCTGACT 
CCCTCCCCAC AGGAAGAACT CCAGCCCATG TTACAGGATT GTCCAGAGGA GACTGGCCAC 
ATGCAGCACC AGCCCGACAG GAGACGGCAG CCTGTGAGTC CTCCTCCACC ACCACGGCCG 
ATCTCCCCTC CACATACCTA TGGCTACATT TCAGGACCCC TGGTCTCAGA TATGGATACA 
GATGCGCCAG AAGAGGAAGA AGACGAAGCC GACATGGAGG TAGCCAAGAT GCAAACCAGA 
AGGCTTTTGT TACGTGGGCT TGAGCAGACA CCTGCCTCCA GTGTTGGGGA CCTGGAGAGC 
TCTGTCACGG GGTCCATGAT CAACGGCTGG GGCTCAGCCT CAGAGGAGGA CAACATTTCC 
AGCGGACGCT CCAGTGTTAG TTCTTCGGAT GGCTCCTTTT TCACTGATGC TGACTTTGCC 
CAGGCAGTCG CAGCAGCGGC AGAGTATGCT GGTCTGAAAG TGGCACGACG GCAAATGCAG 
GATGCTGCTG GCCGTCGACA TTTTCATGCG TCTCAGTGCC CTAGGCCCAC AAGTCCTGTG 
TCTACAGACA GCAACATGAG TGCCGCCGTA ATGCAGAAAA CCAGACCAGC CAAGAAACCG 
AAACACCAGC CAGGACATCT GCGCAGAGAA ACCTACACAG ATGATCTTCC ACCACCTCCT 
GTGCCGCCAC CTGCTATAAA GTCACCTACT GCCCAATCCA AGACACAGCT GGAAGTACGA 
CCTGTAGTGG TGCCAAAACT CCCTTCTGTG GATGCAAGAA CAGACAGATC ATCAGACAGA 
AAAGGAAGCA GTTACAAGGG GAGAGAAGTA TTGGATGGAA GACAGGTTGT TGACATGCGA 
ACAAATCCAG GTGATCCCAG AGAAGCACAG GAACAGCAAA ATGACGGGAA AGGACGTGGA 
AACAAGGCAG CAAAACGAGA CCTTCCACCA GCAAAGACTC ATCTCATCCA AGAGGATATT 
CTACCTTATT GTAGACCTAC TTTTCCAACA TCAAATAATC CCAGAGATCC CAGTTCCTCA 
AGCTCAATGT CATCAAGAGG ATCAGGAAGC AGACAAAGAG AACAAGCAAA TGTAGGTCGA 
AGAAATATTG CAGAAATGCA GGTACTTGGA GGATATGAAA GAGGAGAAGA TAATAATGAA 
GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_024355569.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211337.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc105012
Clone ID Related Accession (Same CDS sequence)	XM_024355569.1
Accession Version	XM_024355569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGAACCTC CACATTTTGT TGTGAAACCC CGTGACCAGG TTGTTGCTTT GGGACGGACT 
GTAACTTTTC AGTGTGAAGC AACCGGAAAT CCTCAACCAG CTATTTTTTG GAGGAGAGAA 
GGGAGTCAGA ATCTACTTTT CTCATATCAA CCACCACAGT CATCCAGCCG ATTTTCAGTC 
TCCCAGACTG GCGACCTCAC AATTACTAAT GTCCAGCGAT CTGATGTTGG TTATTACATC 
TGCCAGACTT TAAATGTTGC TGGAAGCATC ATCACAAAGG CATATTTGGA AGTTACAGAT 
GTGATTGCAG ATCGGCCTCC CCCAGTTATT CGACAAGGTC CTGTGAATCA GACTGTAGCT 
GTGGATGGCA CTTTCGTCCT CAGCTGTGTG GCCACAGGCA GTCCAGTGCC CACCATTCTG 
TGGAGAAAGG ATGGAGTCCT CGTTTCAACC CAAGACTCTC GAATCAAACA GTTGGAGAAT 
GGAGTACTGC AGATCCGATA TGCTAAGCTG GGTGATACTG GTCGGTACAC CTGCATTGCA 
TCAACCCCCA GTGGTGAAGC AACATGGAGT GCTTACATTG AAGTTCAAGA ATTTGGAGTT 
CCAGTTCAGC CTCCAAGACC TACTGACCCA AATTTAATCC CTAGTGCCCC ATCAAAACCT 
GAAGTGACAG ATGTCAGCAG AAATACAGTC ACATTATCAT GGCAACCAAA TTTGAATTCA 
GGAGCAACTC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG 
CAGACCGTAG CAGAGAATGT GAAAACAGAA ACATTTGCCA TTAAAGGACT CAAACCTAAT 
GCAATTTACC TTTTCCTTGT GAGGGCAGCT AATGCATATG GAATTAGTGA TCCAAGCCAA 
ATATCAGATC CAGTGAAAAC ACAAGATGTC CTACCAACAA GTCAGGGGGT GGACCACAAG 
CAGGTCCAGA GAGAGCTGGG GAATGCTGTT CTGCACCTCC ACAACCCCAC CGTCCTTTCT 
TCCTCTTCCA TCGAAGTGCA CTGGACAGTA GATCAACAGT CTCAGTATAT ACAAGGATAT 
AAAATTCTCT ATCGGCCATC TGGAGCCAAC CACGGAGAAT CAGACTGGTT AGTTTTTGAA 
GTGAGGACGC CAGCCAAAAA CAGTGTGGTT ATCCCTGATC TCAGAAAGGG AGTCAACTAT 
GAAATTAAGG CTCGCCCTTT TTTTAATGAA TTTCAAGGAG CAGATAGTGA AATCAAGTTC 
GCCAAAACCC TGGAAGAAGC ACCCAGTGCC CCACCCCAAG GTGTAACTGT ATCCAAGAAT 
GATGGAAACG GAACTGCAAT TCTAGTTAGT TGGCAGCCAC CTCCAGAAGA CACTCAAAAT 
GGAATGGTCC AAGAGTATAA GGTTTGGTGT CTGGGCAATG AAACTCGATA CCACATCAAC 
AAAACAGTGG ATGGTTCCAC CTTTTCCGTG GTCATTCCCT TTCTTGTTCC TGGAATCCGA 
TACAGTGTGG AAGTGGCAGC CAGCACTGGG GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG 
TTCATCCAGC TGGATGCCCA TGGAAACCCT GTGTCACCTG AGGACCAAGT CAGCCTCGCT 
CAGCAGATTT CAGATGTGGT GAAGCAGCCG GCCTTCATAG CAGGTATTGG AGCAGCCTGT 
TGGATCATCC TCATGGTCTT CAGCATCTGG CTTTATCGAC ACCGCAAGAA GAGAAACGGA 
CTTACTAGTA CTTACGCGGG TATCAGAAAA GTCCCGTCTT TTACCTTCAC ACCAACAGTA 
ACTTACCAGA GAGGAGGCGA AGCTGTCAGC AGTGGAGGGA GGCCCGGACT TCTCAACATC 
AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CTAATACTGG CAACAACCAC 
AATGACTGCT CCATCAGCTG CTGCACGGCA GGCAATGGAA ACAGCGACAG CAACCTCACT 
ACCTACAGTC GCCCAGCTGA TTGTATAGCA AATTATAACA ACCAACTGGA TAACAAACAA 
ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC 
AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGATG GGCGTTTTGT CAATCCATCA 
GGGCAGCCTA CTCCTTATGC CACCACTCAG CTCATCCAGT CAAACCTCAG CAACAACATG 
AACAATGGCA GCGGGGACTC TGGCGAGAAG CACTGGAAAC CACTGGGACA GCAGAAACAA 
GAAGTGGCAC CAGTTCAGTA CAACATCATG GAGCAAAACA AGCTGAACAA AGATTATCGA 
GCAAATGACA CAGTTCCTCA AACTATCCCA TACAACCAAT CATACGACCA GAACACAGGA 
GGATCCTACA ACAGCTCAGA CCGGGGCAGT AGTACATCTG GGAGTCAGGG GCACAAGAAA 
GGGGCAAGAA CACCCAAGGT ACCAAAACAG GGTGGCATGA ACTGGGCAGA CCTGCTTCCT 
CCTCCCCCAG CACATCCTCC TCCACACAGC AATAGCGAAG AGTACAACAT TTCTGTAGAT 
GAAAGCTATG ACCAAGAAAT GCCATGTCCC GTGCCACCAG CAAGGATGTA TTTGCAACAA 
GATGAATTAG AAGAGGAGGA AGATGAACGA GGCCCCACTC CCCCCGTTCG GGGAGCAGCT 
TCTTCTCCAG CTGCCGTGTC CTATAGCCAT CAGTCCACTG CCACTCTGAC TCCCTCCCCA 
CAGGAAGAAC TCCAGCCCAT GTTACAGGAT TGTCCAGAGG AGACTGGCCA CATGCAGCAC 
CAGCCCGACA GGAGACGGCA GCCTGTGAGT CCTCCTCCAC CACCACGGCC GATCTCCCCT 
CCACATACCT ATGGCTACAT TTCAGGACCC CTGGTCTCAG ATATGGATAC AGATGCGCCA 
GAAGAGGAAG AAGACGAAGC CGACATGGAG GTAGCCAAGA TGCAAACCAG AAGGCTTTTG 
TTACGTGGGC TTGAGCAGAC ACCTGCCTCC AGTGTTGGGG ACCTGGAGAG CTCTGTCACG 
GGGTCCATGA TCAACGGCTG GGGCTCAGCC TCAGAGGAGG ACAACATTTC CAGCGGACGC 
TCCAGTGTTA GTTCTTCGGA TGGCTCCTTT TTCACTGATG CTGACTTTGC CCAGGCAGTC 
GCAGCAGCGG CAGAGTATGC TGGTCTGAAA GTGGCACGAC GGCAAATGCA GGATGCTGCT 
GGCCGTCGAC ATTTTCATGC GTCTCAGTGC CCTAGGCCCA CAAGTCCTGT GTCTACAGAC 
AGCAACATGA GTGCCGCCGT AATGCAGAAA ACCAGACCAG CCAAGAAACC GAAACACCAG 
CCAGGACATC TGCGCAGAGA AACCTACACA GATGATCTTC CACCACCTCC TGTGCCGCCA 
CCTGCTATAA AGTCACCTAC TGCCCAATCC AAGACACAGC TGGAAGTACG ACCTGTAGTG 
GTGCCAAAAC TCCCTTCTGT GGATGCAAGA ACAGACAGAT CATCAGACAG AAAAGGAAGC 
AGTTACAAGG GGAGAGAAGT ATTGGATGGA AGACAGGTTG TTGACATGCG AACAAATCCA 
GGTGATCCCA GAGAAGCACA GGAACAGCAA AATGACGGGA AAGGACGTGG AAACAAGGCA 
GCAAAACGAG ACCTTCCACC AGCAAAGACT CATCTCATCC AAGAGGATAT TCTACCTTAT 
TGTAGACCTA CTTTTCCAAC ATCAAATAAT CCCAGAGATC CCAGTTCCTC AAGCTCAATG 
TCATCAAGAG GATCAGGAAG CAGACAAAGA GAACAAGCAA ATGTAGGTCG AAGAAATATT 
GCAGAAATGC AGGTACTTGG AGGATATGAA AGAGGAGAAG ATAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211337.1
CDS	54..5108
Translation	MMLRPSQGLVCRRRGKDEACCVLSLCVFLSHVDLLVSGHAVIEMATTVTTVTPSAALVLFPTDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_024355569.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024355569.1

ORF Insert Sequence:

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGAACCTC CACATTTTGT TGTGAAACCC CGTGACCAGG TTGTTGCTTT GGGACGGACT 
GTAACTTTTC AGTGTGAAGC AACCGGAAAT CCTCAACCAG CTATTTTTTG GAGGAGAGAA 
GGGAGTCAGA ATCTACTTTT CTCATATCAA CCACCACAGT CATCCAGCCG ATTTTCAGTC 
TCCCAGACTG GCGACCTCAC AATTACTAAT GTCCAGCGAT CTGATGTTGG TTATTACATC 
TGCCAGACTT TAAATGTTGC TGGAAGCATC ATCACAAAGG CATATTTGGA AGTTACAGAT 
GTGATTGCAG ATCGGCCTCC CCCAGTTATT CGACAAGGTC CTGTGAATCA GACTGTAGCT 
GTGGATGGCA CTTTCGTCCT CAGCTGTGTG GCCACAGGCA GTCCAGTGCC CACCATTCTG 
TGGAGAAAGG ATGGAGTCCT CGTTTCAACC CAAGACTCTC GAATCAAACA GTTGGAGAAT 
GGAGTACTGC AGATCCGATA TGCTAAGCTG GGTGATACTG GTCGGTACAC CTGCATTGCA 
TCAACCCCCA GTGGTGAAGC AACATGGAGT GCTTACATTG AAGTTCAAGA ATTTGGAGTT 
CCAGTTCAGC CTCCAAGACC TACTGACCCA AATTTAATCC CTAGTGCCCC ATCAAAACCT 
GAAGTGACAG ATGTCAGCAG AAATACAGTC ACATTATCAT GGCAACCAAA TTTGAATTCA 
GGAGCAACTC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG 
CAGACCGTAG CAGAGAATGT GAAAACAGAA ACATTTGCCA TTAAAGGACT CAAACCTAAT 
GCAATTTACC TTTTCCTTGT GAGGGCAGCT AATGCATATG GAATTAGTGA TCCAAGCCAA 
ATATCAGATC CAGTGAAAAC ACAAGATGTC CTACCAACAA GTCAGGGGGT GGACCACAAG 
CAGGTCCAGA GAGAGCTGGG GAATGCTGTT CTGCACCTCC ACAACCCCAC CGTCCTTTCT 
TCCTCTTCCA TCGAAGTGCA CTGGACAGTA GATCAACAGT CTCAGTATAT ACAAGGATAT 
AAAATTCTCT ATCGGCCATC TGGAGCCAAC CACGGAGAAT CAGACTGGTT AGTTTTTGAA 
GTGAGGACGC CAGCCAAAAA CAGTGTGGTT ATCCCTGATC TCAGAAAGGG AGTCAACTAT 
GAAATTAAGG CTCGCCCTTT TTTTAATGAA TTTCAAGGAG CAGATAGTGA AATCAAGTTC 
GCCAAAACCC TGGAAGAAGC ACCCAGTGCC CCACCCCAAG GTGTAACTGT ATCCAAGAAT 
GATGGAAACG GAACTGCAAT TCTAGTTAGT TGGCAGCCAC CTCCAGAAGA CACTCAAAAT 
GGAATGGTCC AAGAGTATAA GGTTTGGTGT CTGGGCAATG AAACTCGATA CCACATCAAC 
AAAACAGTGG ATGGTTCCAC CTTTTCCGTG GTCATTCCCT TTCTTGTTCC TGGAATCCGA 
TACAGTGTGG AAGTGGCAGC CAGCACTGGG GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG 
TTCATCCAGC TGGATGCCCA TGGAAACCCT GTGTCACCTG AGGACCAAGT CAGCCTCGCT 
CAGCAGATTT CAGATGTGGT GAAGCAGCCG GCCTTCATAG CAGGTATTGG AGCAGCCTGT 
TGGATCATCC TCATGGTCTT CAGCATCTGG CTTTATCGAC ACCGCAAGAA GAGAAACGGA 
CTTACTAGTA CTTACGCGGG TATCAGAAAA GTCCCGTCTT TTACCTTCAC ACCAACAGTA 
ACTTACCAGA GAGGAGGCGA AGCTGTCAGC AGTGGAGGGA GGCCCGGACT TCTCAACATC 
AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CTAATACTGG CAACAACCAC 
AATGACTGCT CCATCAGCTG CTGCACGGCA GGCAATGGAA ACAGCGACAG CAACCTCACT 
ACCTACAGTC GCCCAGCTGA TTGTATAGCA AATTATAACA ACCAACTGGA TAACAAACAA 
ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC 
AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGATG GGCGTTTTGT CAATCCATCA 
GGGCAGCCTA CTCCTTATGC CACCACTCAG CTCATCCAGT CAAACCTCAG CAACAACATG 
AACAATGGCA GCGGGGACTC TGGCGAGAAG CACTGGAAAC CACTGGGACA GCAGAAACAA 
GAAGTGGCAC CAGTTCAGTA CAACATCATG GAGCAAAACA AGCTGAACAA AGATTATCGA 
GCAAATGACA CAGTTCCTCA AACTATCCCA TACAACCAAT CATACGACCA GAACACAGGA 
GGATCCTACA ACAGCTCAGA CCGGGGCAGT AGTACATCTG GGAGTCAGGG GCACAAGAAA 
GGGGCAAGAA CACCCAAGGT ACCAAAACAG GGTGGCATGA ACTGGGCAGA CCTGCTTCCT 
CCTCCCCCAG CACATCCTCC TCCACACAGC AATAGCGAAG AGTACAACAT TTCTGTAGAT 
GAAAGCTATG ACCAAGAAAT GCCATGTCCC GTGCCACCAG CAAGGATGTA TTTGCAACAA 
GATGAATTAG AAGAGGAGGA AGATGAACGA GGCCCCACTC CCCCCGTTCG GGGAGCAGCT 
TCTTCTCCAG CTGCCGTGTC CTATAGCCAT CAGTCCACTG CCACTCTGAC TCCCTCCCCA 
CAGGAAGAAC TCCAGCCCAT GTTACAGGAT TGTCCAGAGG AGACTGGCCA CATGCAGCAC 
CAGCCCGACA GGAGACGGCA GCCTGTGAGT CCTCCTCCAC CACCACGGCC GATCTCCCCT 
CCACATACCT ATGGCTACAT TTCAGGACCC CTGGTCTCAG ATATGGATAC AGATGCGCCA 
GAAGAGGAAG AAGACGAAGC CGACATGGAG GTAGCCAAGA TGCAAACCAG AAGGCTTTTG 
TTACGTGGGC TTGAGCAGAC ACCTGCCTCC AGTGTTGGGG ACCTGGAGAG CTCTGTCACG 
GGGTCCATGA TCAACGGCTG GGGCTCAGCC TCAGAGGAGG ACAACATTTC CAGCGGACGC 
TCCAGTGTTA GTTCTTCGGA TGGCTCCTTT TTCACTGATG CTGACTTTGC CCAGGCAGTC 
GCAGCAGCGG CAGAGTATGC TGGTCTGAAA GTGGCACGAC GGCAAATGCA GGATGCTGCT 
GGCCGTCGAC ATTTTCATGC GTCTCAGTGC CCTAGGCCCA CAAGTCCTGT GTCTACAGAC 
AGCAACATGA GTGCCGCCGT AATGCAGAAA ACCAGACCAG CCAAGAAACC GAAACACCAG 
CCAGGACATC TGCGCAGAGA AACCTACACA GATGATCTTC CACCACCTCC TGTGCCGCCA 
CCTGCTATAA AGTCACCTAC TGCCCAATCC AAGACACAGC TGGAAGTACG ACCTGTAGTG 
GTGCCAAAAC TCCCTTCTGT GGATGCAAGA ACAGACAGAT CATCAGACAG AAAAGGAAGC 
AGTTACAAGG GGAGAGAAGT ATTGGATGGA AGACAGGTTG TTGACATGCG AACAAATCCA 
GGTGATCCCA GAGAAGCACA GGAACAGCAA AATGACGGGA AAGGACGTGG AAACAAGGCA 
GCAAAACGAG ACCTTCCACC AGCAAAGACT CATCTCATCC AAGAGGATAT TCTACCTTAT 
TGTAGACCTA CTTTTCCAAC ATCAAATAAT CCCAGAGATC CCAGTTCCTC AAGCTCAATG 
TCATCAAGAG GATCAGGAAG CAGACAAAGA GAACAAGCAA ATGTAGGTCG AAGAAATATT 
GCAGAAATGC AGGTACTTGG AGGATATGAA AGAGGAGAAG ATAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_024355570.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211338.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

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CloneID	OPc105013
Clone ID Related Accession (Same CDS sequence)	XM_024355570.1
Accession Version	XM_024355570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TAACTTACCA GAGAGGAGGC 
GAAGCTGTCA GCAGTGGAGG GAGGCCCGGA CTTCTCAACA TCAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCTAATACT GGCAACAACC ACAATGACTG CTCCATCAGC 
TGCTGCACGG CAGGCAATGG AAACAGCGAC AGCAACCTCA CTACCTACAG TCGCCCAGCT 
GATTGTATAG CAAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA TGGGCGTTTT GTCAATCCAT CAGGGCAGCC TACTCCTTAT 
GCCACCACTC AGCTCATCCA GTCAAACCTC AGCAACAACA TGAACAATGG CAGCGGGGAC 
TCTGGCGAGA AGCACTGGAA ACCACTGGGA CAGCAGAAAC AAGAAGTGGC ACCAGTTCAG 
TACAACATCA TGGAGCAAAA CAAGCTGAAC AAAGGTGAAG AGGCTTATTA TCGAGCAAAT 
GACACAGTTC CTCAAACTAT CCCATACAAC CAATCATACG ACCAGAACAC AGGAGGATCC 
TACAACAGCT CAGACCGGGG CAGTAGTACA TCTGGGAGTC AGGGGCACAA GAAAGGGGCA 
AGAACACCCA AGGTACCAAA ACAGGGTGGC ATGAACTGGG CAGACCTGCT TCCTCCTCCC 
CCAGCACATC CTCCTCCACA CAGCAATAGC GAAGAGTACA ACATTTCTGT AGATGAAAGC 
TATGACCAAG AAATGCCATG TCCCGTGCCA CCAGCAAGGA TGTATTTGCA ACAAGATGAA 
TTAGAAGAGG AGGAAGATGA ACGAGGCCCC ACTCCCCCCG TTCGGGGAGC AGCTTCTTCT 
CCAGCTGCCG TGTCCTATAG CCATCAGTCC ACTGCCACTC TGACTCCCTC CCCACAGGAA 
GAACTCCAGC CCATGTTACA GGATTGTCCA GAGGAGACTG GCCACATGCA GCACCAGCCC 
GACAGGAGAC GGCAGCCTGT GAGTCCTCCT CCACCACCAC GGCCGATCTC CCCTCCACAT 
ACCTATGGCT ACATTTCAGG ACCCCTGGTC TCAGATATGG ATACAGATGC GCCAGAAGAG 
GAAGAAGACG AAGCCGACAT GGAGGTAGCC AAGATGCAAA CCAGAAGGCT TTTGTTACGT 
GGGCTTGAGC AGACACCTGC CTCCAGTGTT GGGGACCTGG AGAGCTCTGT CACGGGGTCC 
ATGATCAACG GCTGGGGCTC AGCCTCAGAG GAGGACAACA TTTCCAGCGG ACGCTCCAGT 
GTTAGTTCTT CGGATGGCTC CTTTTTCACT GATGCTGACT TTGCCCAGGC AGTCGCAGCA 
GCGGCAGAGT ATGCTGGTCT GAAAGTGGCA CGACGGCAAA TGCAGGATGC TGCTGGCCGT 
CGACATTTTC ATGCGTCTCA GTGCCCTAGG CCCACAAGTC CTGTGTCTAC AGACAGCAAC 
ATGAGTGCCG CCGTAATGCA GAAAACCAGA CCAGCCAAGA AACCGAAACA CCAGCCAGGA 
CATCTGCGCA GAGAAACCTA CACAGATGAT CTTCCACCAC CTCCTGTGCC GCCACCTGCT 
ATAAAGTCAC CTACTGCCCA ATCCAAGACA CAGCTGGAAG TACGACCTGT AGTGGTGCCA 
AAACTCCCTT CTGTGGATGC AAGAACAGAC AGATCATCAG ACAGAAAAGG AAGCAGTTAC 
AAGGGGAGAG AAGTATTGGA TGGAAGACAG GTTGTTGACA TGCGAACAAA TCCAGGTGAT 
CCCAGAGAAG CACAGGAACA GCAAAATGAC GGGAAAGGAC GTGGAAACAA GGCAGCAAAA 
CGAGACCTTC CACCAGCAAA GACTCATCTC ATCCAAGAGG ATATTCTACC TTATTGTAGA 
CCTACTTTTC CAACATCAAA TAATCCCAGA GATCCCAGTT CCTCAAGCTC AATGTCATCA 
AGAGGATCAG GAAGCAGACA AAGAGAACAA GCAAATGTAG GTCGAAGAAA TATTGCAGAA 
ATGCAGGTAC TTGGAGGATA TGAAAGAGGA GAAGATAATA ATGAAGAATT AGAGGAAACT 
GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211338.1
CDS	55..5103
Translation	MMLRPSQGLVCRRRGKDEACCVLSLCVFLSHVDLLVSGHAVIEMATTVTTVTPSAALVLFPTDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_024355570.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024355570.1

ORF Insert Sequence:

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TAACTTACCA GAGAGGAGGC 
GAAGCTGTCA GCAGTGGAGG GAGGCCCGGA CTTCTCAACA TCAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCTAATACT GGCAACAACC ACAATGACTG CTCCATCAGC 
TGCTGCACGG CAGGCAATGG AAACAGCGAC AGCAACCTCA CTACCTACAG TCGCCCAGCT 
GATTGTATAG CAAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA TGGGCGTTTT GTCAATCCAT CAGGGCAGCC TACTCCTTAT 
GCCACCACTC AGCTCATCCA GTCAAACCTC AGCAACAACA TGAACAATGG CAGCGGGGAC 
TCTGGCGAGA AGCACTGGAA ACCACTGGGA CAGCAGAAAC AAGAAGTGGC ACCAGTTCAG 
TACAACATCA TGGAGCAAAA CAAGCTGAAC AAAGGTGAAG AGGCTTATTA TCGAGCAAAT 
GACACAGTTC CTCAAACTAT CCCATACAAC CAATCATACG ACCAGAACAC AGGAGGATCC 
TACAACAGCT CAGACCGGGG CAGTAGTACA TCTGGGAGTC AGGGGCACAA GAAAGGGGCA 
AGAACACCCA AGGTACCAAA ACAGGGTGGC ATGAACTGGG CAGACCTGCT TCCTCCTCCC 
CCAGCACATC CTCCTCCACA CAGCAATAGC GAAGAGTACA ACATTTCTGT AGATGAAAGC 
TATGACCAAG AAATGCCATG TCCCGTGCCA CCAGCAAGGA TGTATTTGCA ACAAGATGAA 
TTAGAAGAGG AGGAAGATGA ACGAGGCCCC ACTCCCCCCG TTCGGGGAGC AGCTTCTTCT 
CCAGCTGCCG TGTCCTATAG CCATCAGTCC ACTGCCACTC TGACTCCCTC CCCACAGGAA 
GAACTCCAGC CCATGTTACA GGATTGTCCA GAGGAGACTG GCCACATGCA GCACCAGCCC 
GACAGGAGAC GGCAGCCTGT GAGTCCTCCT CCACCACCAC GGCCGATCTC CCCTCCACAT 
ACCTATGGCT ACATTTCAGG ACCCCTGGTC TCAGATATGG ATACAGATGC GCCAGAAGAG 
GAAGAAGACG AAGCCGACAT GGAGGTAGCC AAGATGCAAA CCAGAAGGCT TTTGTTACGT 
GGGCTTGAGC AGACACCTGC CTCCAGTGTT GGGGACCTGG AGAGCTCTGT CACGGGGTCC 
ATGATCAACG GCTGGGGCTC AGCCTCAGAG GAGGACAACA TTTCCAGCGG ACGCTCCAGT 
GTTAGTTCTT CGGATGGCTC CTTTTTCACT GATGCTGACT TTGCCCAGGC AGTCGCAGCA 
GCGGCAGAGT ATGCTGGTCT GAAAGTGGCA CGACGGCAAA TGCAGGATGC TGCTGGCCGT 
CGACATTTTC ATGCGTCTCA GTGCCCTAGG CCCACAAGTC CTGTGTCTAC AGACAGCAAC 
ATGAGTGCCG CCGTAATGCA GAAAACCAGA CCAGCCAAGA AACCGAAACA CCAGCCAGGA 
CATCTGCGCA GAGAAACCTA CACAGATGAT CTTCCACCAC CTCCTGTGCC GCCACCTGCT 
ATAAAGTCAC CTACTGCCCA ATCCAAGACA CAGCTGGAAG TACGACCTGT AGTGGTGCCA 
AAACTCCCTT CTGTGGATGC AAGAACAGAC AGATCATCAG ACAGAAAAGG AAGCAGTTAC 
AAGGGGAGAG AAGTATTGGA TGGAAGACAG GTTGTTGACA TGCGAACAAA TCCAGGTGAT 
CCCAGAGAAG CACAGGAACA GCAAAATGAC GGGAAAGGAC GTGGAAACAA GGCAGCAAAA 
CGAGACCTTC CACCAGCAAA GACTCATCTC ATCCAAGAGG ATATTCTACC TTATTGTAGA 
CCTACTTTTC CAACATCAAA TAATCCCAGA GATCCCAGTT CCTCAAGCTC AATGTCATCA 
AGAGGATCAG GAAGCAGACA AAGAGAACAA GCAAATGTAG GTCGAAGAAA TATTGCAGAA 
ATGCAGGTAC TTGGAGGATA TGAAAGAGGA GAAGATAATA ATGAAGAATT AGAGGAAACT 
GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_024355571.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211339.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

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CloneID	OPc105014
Clone ID Related Accession (Same CDS sequence)	XM_024355571.1
Accession Version	XM_024355571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TAACTTACCA GAGAGGAGGC 
GAAGCTGTCA GCAGTGGAGG GAGGCCCGGA CTTCTCAACA TCAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCTAATACT GGCAACAACC ACAATGACTG CTCCATCAGC 
TGCTGCACGG CAGGCAATGG AAACAGCGAC AGCAACCTCA CTACCTACAG TCGCCCAGCT 
GATTGTATAG CAAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA TGGGCGTTTT GTCAATCCAT CAGGGCAGCC TACTCCTTAT 
GCCACCACTC AGCTCATCCA GTCAAACCTC AGCAACAACA TGAACAATGG CAGCGGGGAC 
TCTGGCGAGA AGCACTGGAA ACCACTGGGA CAGCAGAAAC AAGAAGTGGC ACCAGTTCAG 
TACAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GAGCAAATGA CACAGTTCCT 
CAAACTATCC CATACAACCA ATCATACGAC CAGAACACAG GAGGATCCTA CAACAGCTCA 
GACCGGGGCA GTAGTACATC TGGGAGTCAG GGGCACAAGA AAGGGGCAAG AACACCCAAG 
GTACCAAAAC AGGGTGGCAT GAACTGGGCA GACCTGCTTC CTCCTCCCCC AGCACATCCT 
CCTCCACACA GCAATAGCGA AGAGTACAAC ATTTCTGTAG ATGAAAGCTA TGACCAAGAA 
ATGCCATGTC CCGTGCCACC AGCAAGGATG TATTTGCAAC AAGATGAATT AGAAGAGGAG 
GAAGATGAAC GAGGCCCCAC TCCCCCCGTT CGGGGAGCAG CTTCTTCTCC AGCTGCCGTG 
TCCTATAGCC ATCAGTCCAC TGCCACTCTG ACTCCCTCCC CACAGGAAGA ACTCCAGCCC 
ATGTTACAGG ATTGTCCAGA GGAGACTGGC CACATGCAGC ACCAGCCCGA CAGGAGACGG 
CAGCCTGTGA GTCCTCCTCC ACCACCACGG CCGATCTCCC CTCCACATAC CTATGGCTAC 
ATTTCAGGAC CCCTGGTCTC AGATATGGAT ACAGATGCGC CAGAAGAGGA AGAAGACGAA 
GCCGACATGG AGGTAGCCAA GATGCAAACC AGAAGGCTTT TGTTACGTGG GCTTGAGCAG 
ACACCTGCCT CCAGTGTTGG GGACCTGGAG AGCTCTGTCA CGGGGTCCAT GATCAACGGC 
TGGGGCTCAG CCTCAGAGGA GGACAACATT TCCAGCGGAC GCTCCAGTGT TAGTTCTTCG 
GATGGCTCCT TTTTCACTGA TGCTGACTTT GCCCAGGCAG TCGCAGCAGC GGCAGAGTAT 
GCTGGTCTGA AAGTGGCACG ACGGCAAATG CAGGATGCTG CTGGCCGTCG ACATTTTCAT 
GCGTCTCAGT GCCCTAGGCC CACAAGTCCT GTGTCTACAG ACAGCAACAT GAGTGCCGCC 
GTAATGCAGA AAACCAGACC AGCCAAGAAA CCGAAACACC AGCCAGGACA TCTGCGCAGA 
GAAACCTACA CAGATGATCT TCCACCACCT CCTGTGCCGC CACCTGCTAT AAAGTCACCT 
ACTGCCCAAT CCAAGACACA GCTGGAAGTA CGACCTGTAG TGGTGCCAAA ACTCCCTTCT 
GTGGATGCAA GAACAGACAG ATCATCAGAC AGAAAAGGAA GCAGTTACAA GGGGAGAGAA 
GTATTGGATG GAAGACAGGT TGTTGACATG CGAACAAATC CAGGTGATCC CAGAGAAGCA 
CAGGAACAGC AAAATGACGG GAAAGGACGT GGAAACAAGG CAGCAAAACG AGACCTTCCA 
CCAGCAAAGA CTCATCTCAT CCAAGAGGAT ATTCTACCTT ATTGTAGACC TACTTTTCCA 
ACATCAAATA ATCCCAGAGA TCCCAGTTCC TCAAGCTCAA TGTCATCAAG AGGATCAGGA 
AGCAGACAAA GAGAACAAGC AAATGTAGGT CGAAGAAATA TTGCAGAAAT GCAGGTACTT 
GGAGGATATG AAAGAGGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211339.1
CDS	53..5089
Translation	MMLRPSQGLVCRRRGKDEACCVLSLCVFLSHVDLLVSGHAVIEMATTVTTVTPSAALVLFPTDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_024355571.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024355571.1

ORF Insert Sequence:

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TAACTTACCA GAGAGGAGGC 
GAAGCTGTCA GCAGTGGAGG GAGGCCCGGA CTTCTCAACA TCAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCTAATACT GGCAACAACC ACAATGACTG CTCCATCAGC 
TGCTGCACGG CAGGCAATGG AAACAGCGAC AGCAACCTCA CTACCTACAG TCGCCCAGCT 
GATTGTATAG CAAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA TGGGCGTTTT GTCAATCCAT CAGGGCAGCC TACTCCTTAT 
GCCACCACTC AGCTCATCCA GTCAAACCTC AGCAACAACA TGAACAATGG CAGCGGGGAC 
TCTGGCGAGA AGCACTGGAA ACCACTGGGA CAGCAGAAAC AAGAAGTGGC ACCAGTTCAG 
TACAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GAGCAAATGA CACAGTTCCT 
CAAACTATCC CATACAACCA ATCATACGAC CAGAACACAG GAGGATCCTA CAACAGCTCA 
GACCGGGGCA GTAGTACATC TGGGAGTCAG GGGCACAAGA AAGGGGCAAG AACACCCAAG 
GTACCAAAAC AGGGTGGCAT GAACTGGGCA GACCTGCTTC CTCCTCCCCC AGCACATCCT 
CCTCCACACA GCAATAGCGA AGAGTACAAC ATTTCTGTAG ATGAAAGCTA TGACCAAGAA 
ATGCCATGTC CCGTGCCACC AGCAAGGATG TATTTGCAAC AAGATGAATT AGAAGAGGAG 
GAAGATGAAC GAGGCCCCAC TCCCCCCGTT CGGGGAGCAG CTTCTTCTCC AGCTGCCGTG 
TCCTATAGCC ATCAGTCCAC TGCCACTCTG ACTCCCTCCC CACAGGAAGA ACTCCAGCCC 
ATGTTACAGG ATTGTCCAGA GGAGACTGGC CACATGCAGC ACCAGCCCGA CAGGAGACGG 
CAGCCTGTGA GTCCTCCTCC ACCACCACGG CCGATCTCCC CTCCACATAC CTATGGCTAC 
ATTTCAGGAC CCCTGGTCTC AGATATGGAT ACAGATGCGC CAGAAGAGGA AGAAGACGAA 
GCCGACATGG AGGTAGCCAA GATGCAAACC AGAAGGCTTT TGTTACGTGG GCTTGAGCAG 
ACACCTGCCT CCAGTGTTGG GGACCTGGAG AGCTCTGTCA CGGGGTCCAT GATCAACGGC 
TGGGGCTCAG CCTCAGAGGA GGACAACATT TCCAGCGGAC GCTCCAGTGT TAGTTCTTCG 
GATGGCTCCT TTTTCACTGA TGCTGACTTT GCCCAGGCAG TCGCAGCAGC GGCAGAGTAT 
GCTGGTCTGA AAGTGGCACG ACGGCAAATG CAGGATGCTG CTGGCCGTCG ACATTTTCAT 
GCGTCTCAGT GCCCTAGGCC CACAAGTCCT GTGTCTACAG ACAGCAACAT GAGTGCCGCC 
GTAATGCAGA AAACCAGACC AGCCAAGAAA CCGAAACACC AGCCAGGACA TCTGCGCAGA 
GAAACCTACA CAGATGATCT TCCACCACCT CCTGTGCCGC CACCTGCTAT AAAGTCACCT 
ACTGCCCAAT CCAAGACACA GCTGGAAGTA CGACCTGTAG TGGTGCCAAA ACTCCCTTCT 
GTGGATGCAA GAACAGACAG ATCATCAGAC AGAAAAGGAA GCAGTTACAA GGGGAGAGAA 
GTATTGGATG GAAGACAGGT TGTTGACATG CGAACAAATC CAGGTGATCC CAGAGAAGCA 
CAGGAACAGC AAAATGACGG GAAAGGACGT GGAAACAAGG CAGCAAAACG AGACCTTCCA 
CCAGCAAAGA CTCATCTCAT CCAAGAGGAT ATTCTACCTT ATTGTAGACC TACTTTTCCA 
ACATCAAATA ATCCCAGAGA TCCCAGTTCC TCAAGCTCAA TGTCATCAAG AGGATCAGGA 
AGCAGACAAA GAGAACAAGC AAATGTAGGT CGAAGAAATA TTGCAGAAAT GCAGGTACTT 
GGAGGATATG AAAGAGGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941496.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796985.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

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CloneID	OPc105015
Clone ID Related Accession (Same CDS sequence)	XM_016941496.2
Accession Version	XM_016941496.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016941496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG GAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCCGGACT TCTCAACATC AGTGAACCTG CCACGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTATGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCATG 
GAGCAAAACA AGCTGAACAA AGGTGAAGAG GCTTATTATC GAGCAAATGA CACAGTTCCT 
CAAACTATCC CATACAACCA ATCATACGAC CAGAACACAG GAGGATCCTA CAACAGCTCA 
GACCGGGGCA GTAGTACATC TGGGAGTCAG GGGCACAAGA AAGGGGCAAG AACACCCAAG 
GTACCAAAAC AGGGTGGCAT GAACTGGGCA GACCTGCTTC CTCCTCCCCC AGCACATCCT 
CCTCCACACA GCAATAGCGA AGAGTACAAC ATTTCTGTAG ATGAAAGCTA TGACCAAGAA 
ATGCCATGTC CCGTGCCACC AGCAAGGATG TATTTGCAAC AAGATGAATT AGAAGAGGAG 
GAAGATGAAC GAGGCCCCAC TCCCCCCGTT CGGGGAGCAG CTTCTTCTCC AGCTGCCGTG 
TCCTATAGCC ATCAGTCCAC TGCCACTCTG ACTCCCTCCC CACAGGAAGA ACTCCAGCCC 
ATGTTACAGG ATTGTCCAGA GGAGACTGGC CACATGCAGC ACCAGCCCGA CAGGAGACGG 
CAGCCTGTGA GTCCTCCTCC ACCACCACGG CCGATCTCCC CTCCACATAC CTATGGCTAC 
ATTTCAGGAC CCCTGGTCTC AGATATGGAT ACAGATGCGC CAGAAGAGGA AGAAGACGAA 
GCCGACATGG AGGTAGCCAA GATGCAAACC AGAAGGCTTT TGTTACGTGG GCTTGAGCAG 
ACACCTGCCT CCAGTGTTGG GGACCTGGAG AGCTCTGTCA CGGGGTCCAT GATCAACGGC 
TGGGGCTCAG CCTCAGAGGA GGACAACATT TCCAGCGGAC GCTCCAGTGT TAGTTCTTCG 
GATGGCTCCT TTTTCACTGA TGCTGACTTT GCCCAGGCAG TCGCAGCAGC GGCAGAGTAT 
GCTGGTCTGA AAGTGGCACG ACGGCAAATG CAGGATGCTG CTGGCCGTCG ACATTTTCAT 
GCGTCTCAGT GCCCTAGGCC CACAAGTCCT GTGTCTACAG ACAGCAACAT GAGTGCCGCC 
GTAATGCAGA AAACCAGACC AGCCAAGAAA CCGAAACACC AGCCAGGACA TCTGCGCAGA 
GAAACCTACA CAGATGATCT TCCACCACCT CCTGTGCCGC CACCTGCTAT AAAGTCACCT 
ACTGCCCAAT CCAAGACACA GCTGGAAGTA CGACCTGTAG TGGTGCCAAA ACTCCCTTCT 
GTGGATGCAA GAACAGACAG ATCATCAGAC AGAAAAGGAA GCAGTTACAA GGGGAGAGAA 
GTATTGGATG GAAGACAGGT TGTTGACATG CGAACAAATC CAGGTGATCC CAGAGAAGCA 
CAGGAACAGC AAAATGACGG GAAAGGACGT GGAAACAAGG CAGCAAAACG AGACCTTCCA 
CCAGCAAAGA CTCATCTCAT CCAAGAGGAT ATTCTACCTT ATTGTAGACC TACTTTTCCA 
ACATCAAATA ATCCCAGAGA TCCCAGTTCC TCAAGCTCAA TGTCATCAAG AGGATCAGGA 
AGCAGACAAA GAGAACAAGC AAATGTAGGT CGAAGAAATA TTGCAGAAAT GCAGGTACTT 
GGAGGATATG AAAGAGGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796985.1
CDS	293..5269
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941496.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941496.2

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG GAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCCGGACT TCTCAACATC AGTGAACCTG CCACGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTATGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCATG 
GAGCAAAACA AGCTGAACAA AGGTGAAGAG GCTTATTATC GAGCAAATGA CACAGTTCCT 
CAAACTATCC CATACAACCA ATCATACGAC CAGAACACAG GAGGATCCTA CAACAGCTCA 
GACCGGGGCA GTAGTACATC TGGGAGTCAG GGGCACAAGA AAGGGGCAAG AACACCCAAG 
GTACCAAAAC AGGGTGGCAT GAACTGGGCA GACCTGCTTC CTCCTCCCCC AGCACATCCT 
CCTCCACACA GCAATAGCGA AGAGTACAAC ATTTCTGTAG ATGAAAGCTA TGACCAAGAA 
ATGCCATGTC CCGTGCCACC AGCAAGGATG TATTTGCAAC AAGATGAATT AGAAGAGGAG 
GAAGATGAAC GAGGCCCCAC TCCCCCCGTT CGGGGAGCAG CTTCTTCTCC AGCTGCCGTG 
TCCTATAGCC ATCAGTCCAC TGCCACTCTG ACTCCCTCCC CACAGGAAGA ACTCCAGCCC 
ATGTTACAGG ATTGTCCAGA GGAGACTGGC CACATGCAGC ACCAGCCCGA CAGGAGACGG 
CAGCCTGTGA GTCCTCCTCC ACCACCACGG CCGATCTCCC CTCCACATAC CTATGGCTAC 
ATTTCAGGAC CCCTGGTCTC AGATATGGAT ACAGATGCGC CAGAAGAGGA AGAAGACGAA 
GCCGACATGG AGGTAGCCAA GATGCAAACC AGAAGGCTTT TGTTACGTGG GCTTGAGCAG 
ACACCTGCCT CCAGTGTTGG GGACCTGGAG AGCTCTGTCA CGGGGTCCAT GATCAACGGC 
TGGGGCTCAG CCTCAGAGGA GGACAACATT TCCAGCGGAC GCTCCAGTGT TAGTTCTTCG 
GATGGCTCCT TTTTCACTGA TGCTGACTTT GCCCAGGCAG TCGCAGCAGC GGCAGAGTAT 
GCTGGTCTGA AAGTGGCACG ACGGCAAATG CAGGATGCTG CTGGCCGTCG ACATTTTCAT 
GCGTCTCAGT GCCCTAGGCC CACAAGTCCT GTGTCTACAG ACAGCAACAT GAGTGCCGCC 
GTAATGCAGA AAACCAGACC AGCCAAGAAA CCGAAACACC AGCCAGGACA TCTGCGCAGA 
GAAACCTACA CAGATGATCT TCCACCACCT CCTGTGCCGC CACCTGCTAT AAAGTCACCT 
ACTGCCCAAT CCAAGACACA GCTGGAAGTA CGACCTGTAG TGGTGCCAAA ACTCCCTTCT 
GTGGATGCAA GAACAGACAG ATCATCAGAC AGAAAAGGAA GCAGTTACAA GGGGAGAGAA 
GTATTGGATG GAAGACAGGT TGTTGACATG CGAACAAATC CAGGTGATCC CAGAGAAGCA 
CAGGAACAGC AAAATGACGG GAAAGGACGT GGAAACAAGG CAGCAAAACG AGACCTTCCA 
CCAGCAAAGA CTCATCTCAT CCAAGAGGAT ATTCTACCTT ATTGTAGACC TACTTTTCCA 
ACATCAAATA ATCCCAGAGA TCCCAGTTCC TCAAGCTCAA TGTCATCAAG AGGATCAGGA 
AGCAGACAAA GAGAACAAGC AAATGTAGGT CGAAGAAATA TTGCAGAAAT GCAGGTACTT 
GGAGGATATG AAAGAGGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941499.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796988.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

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CloneID	OPc105016
Clone ID Related Accession (Same CDS sequence)	XM_016941499.2
Accession Version	XM_016941499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016941499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTTT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC TGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCA 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATTTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GGAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT TATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT CGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCCGGAC TTCTCAACAT CAGTGAACCT GCCACGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796988.1
CDS	209..5164
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941499.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941499.2

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTTT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC TGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCA 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATTTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GGAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT TATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT CGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCCGGAC TTCTCAACAT CAGTGAACCT GCCACGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941502.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796991.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

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CloneID	OPc105017
Clone ID Related Accession (Same CDS sequence)	XM_016941502.2
Accession Version	XM_016941502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016941502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGC TGTTACTGAT GCTGCTGCTC TCGATGGCTG AGATAACTTC TCCTGTGGGT 
GTCTTGGACA GAACGAAAGC TGGGTCAAGG TGGAAAGGTG AAAAGAATCG CTCCCGTCTT 
CGTCAGGAAG ATTTTCCACC TCGCATTGTT GAACACCCTT CAGACCTGAT TGTCTCAAAA 
GGAGAACCTG CAACTTTGAA CTGCAAAGCT GAAGGCCGCC CCACACCCAC TATTGAATGG 
TACAAAGGGG GAGAGAGAGT GGAGACAGAC AAAGATGACC CTCGCTCACA CCGAATGTTG 
CTGCCGAGTG GATCTTTATT TTTCTTACGT ATAGTACATG GACGGAAAAG TAGACCTGAT 
GAAGGAGTCT ATGTCTGTGT AGCAAGGAAT TACCTTGGAG AGGCTGTGAG CCACAATGCA 
TCGCTGGAAG TAGCCATACT TCGGGATGAC TTCAGACAAA ACCCTTCGGA TGTCATGGTT 
GCAGTAGGAG AGCCTGCAGT AATGGAATGC CAACCTCCAC GAGGCCATCC TGAGCCCACC 
ATTTCATGGA AGAAAGATGG CTCTCCACTG GATGATAAAG ATGAAAGAAT AACTATACGA 
GGAGGAAAGC TCATGATCAC TTACACCCGT AAAAGTGACG CTGGCAAATA TGTTTGTGTT 
GGTACCAATA TGGTTGGGGA ACGTGAGAGT GAAGTAGCCG AGCTGACTGT CTTAGAGAGA 
CCATCATTTG TGAAGAGACC CAGTAACTTG GCAGTAACTG TGGATGACAG TGCAGAATTT 
AAATGTGAGG CCCGAGGTGA CCCTGTACCT ACAGTACGAT GGAGGAAAGA TGATGGAGAG 
CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACCT TGAAAATTAG GAAGGTGACA 
GCTGGTGACA TGGGTTCATA CACTTGTGTT GCAGAAAATA TGGTGGGCAA AGCTGAAGCA 
TCTGCTACTC TGACTGTGCA AGTTGAGTCT GAACCTCCAC ATTTTGTTGT GAAACCCCGT 
GACCAGGTTG TTGCTTTGGG ACGGACTGTA ACTTTTCAGT GTGAAGCAAC CGGAAATCCT 
CAACCAGCTA TTTTTTGGAG GAGAGAAGGG AGTCAGAATC TACTTTTCTC ATATCAACCA 
CCACAGTCAT CCAGCCGATT TTCAGTCTCC CAGACTGGCG ACCTCACAAT TACTAATGTC 
CAGCGATCTG ATGTTGGTTA TTACATCTGC CAGACTTTAA ATGTTGCTGG AAGCATCATC 
ACAAAGGCAT ATTTGGAAGT TACAGATGTG ATTGCAGATC GGCCTCCCCC AGTTATTCGA 
CAAGGTCCTG TGAATCAGAC TGTAGCTGTG GATGGCACTT TCGTCCTCAG CTGTGTGGCC 
ACAGGCAGTC CAGTGCCCAC CATTCTGTGG AGAAAGGATG GAGTCCTCGT TTCAACCCAA 
GACTCTCGAA TCAAACAGTT GGAGAATGGA GTACTGCAGA TCCGATATGC TAAGCTGGGT 
GATACTGGTC GGTACACCTG CATTGCATCA ACCCCCAGTG GTGAAGCAAC ATGGAGTGCT 
TACATTGAAG TTCAAGAATT TGGAGTTCCA GTTCAGCCTC CAAGACCTAC TGACCCAAAT 
TTAATCCCTA GTGCCCCATC AAAACCTGAA GTGACAGATG TCAGCAGAAA TACAGTCACA 
TTATCATGGC AACCAAATTT GAATTCAGGA GCAACTCCAA CATCTTATAT TATAGAAGCC 
TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACCGTAGCAG AGAATGTGAA AACAGAAACA 
TTTGCCATTA AAGGACTCAA ACCTAATGCA ATTTACCTTT TCCTTGTGAG GGCAGCTAAT 
GCATATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACACA AGATGTCCTA 
CCAACAAGTC AGGGGGTGGA CCACAAGCAG GTCCAGAGAG AGCTGGGGAA TGCTGTTCTG 
CACCTCCACA ACCCCACCGT CCTTTCTTCC TCTTCCATCG AAGTGCACTG GACAGTAGAT 
CAACAGTCTC AGTATATACA AGGATATAAA ATTCTCTATC GGCCATCTGG AGCCAACCAC 
GGAGAATCAG ACTGGTTAGT TTTTGAAGTG AGGACGCCAG CCAAAAACAG TGTGGTTATC 
CCTGATCTCA GAAAGGGAGT CAACTATGAA ATTAAGGCTC GCCCTTTTTT TAATGAATTT 
CAAGGAGCAG ATAGTGAAAT CAAGTTCGCC AAAACCCTGG AAGAAGCACC CAGTGCCCCA 
CCCCAAGGTG TAACTGTATC CAAGAATGAT GGAAACGGAA CTGCAATTCT AGTTAGTTGG 
CAGCCACCTC CAGAAGACAC TCAAAATGGA ATGGTCCAAG AGTATAAGGT TTGGTGTCTG 
GGCAATGAAA CTCGATACCA CATCAACAAA ACAGTGGATG GTTCCACCTT TTCCGTGGTC 
ATTCCCTTTC TTGTTCCTGG AATCCGATAC AGTGTGGAAG TGGCAGCCAG CACTGGGGCT 
GGGTCTGGGG TAAAGAGTGA GCCTCAGTTC ATCCAGCTGG ATGCCCATGG AAACCCTGTG 
TCACCTGAGG ACCAAGTCAG CCTCGCTCAG CAGATTTCAG ATGTGGTGAA GCAGCCGGCC 
TTCATAGCAG GTATTGGAGC AGCCTGTTGG ATCATCCTCA TGGTCTTCAG CATCTGGCTT 
TATCGACACC GCAAGAAGAG AAACGGACTT ACTAGTACTT ACGCGGGTAT CAGAAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTAACT TACCAGAGAG GAGGCGAAGC TGTCAGCAGT 
GGAGGGAGGC CCGGACTTCT CAACATCAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC 
ACGTGGCCTA ATACTGGCAA CAACCACAAT GACTGCTCCA TCAGCTGCTG CACGGCAGGC 
AATGGAAACA GCGACAGCAA CCTCACTACC TACAGTCGCC CAGCTGATTG TATAGCAAAT 
TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT 
GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG 
AAGGATGGGC GTTTTGTCAA TCCATCAGGG CAGCCTACTC CTTATGCCAC CACTCAGCTC 
ATCCAGTCAA ACCTCAGCAA CAACATGAAC AATGGCAGCG GGGACTCTGG CGAGAAGCAC 
TGGAAACCAC TGGGACAGCA GAAACAAGAA GTGGCACCAG TTCAGTACAA CATCATGGAG 
CAAAACAAGC TGAACAAAGG TGAAGAGGCT TATTATCGAG CAAATGACAC AGTTCCTCAA 
ACTATCCCAT ACAACCAATC ATACGACCAG AACACAGGAG GATCCTACAA CAGCTCAGAC 
CGGGGCAGTA GTACATCTGG GAGTCAGGGG CACAAGAAAG GGGCAAGAAC ACCCAAGGTA 
CCAAAACAGG GTGGCATGAA CTGGGCAGAC CTGCTTCCTC CTCCCCCAGC ACATCCTCCT 
CCACACAGCA ATAGCGAAGA GTACAACATT TCTGTAGATG AAAGCTATGA CCAAGAAATG 
CCATGTCCCG TGCCACCAGC AAGGATGTAT TTGCAACAAG ATGAATTAGA AGAGGAGGAA 
GATGAACGAG GCCCCACTCC CCCCGTTCGG GGAGCAGCTT CTTCTCCAGC TGCCGTGTCC 
TATAGCCATC AGTCCACTGC CACTCTGACT CCCTCCCCAC AGGAAGAACT CCAGCCCATG 
TTACAGGATT GTCCAGAGGA GACTGGCCAC ATGCAGCACC AGCCCGACAG GAGACGGCAG 
CCTGTGAGTC CTCCTCCACC ACCACGGCCG ATCTCCCCTC CACATACCTA TGGCTACATT 
TCAGGACCCC TGGTCTCAGA TATGGATACA GATGCGCCAG AAGAGGAAGA AGACGAAGCC 
GACATGGAGG TAGCCAAGAT GCAAACCAGA AGGCTTTTGT TACGTGGGCT TGAGCAGACA 
CCTGCCTCCA GTGTTGGGGA CCTGGAGAGC TCTGTCACGG GGTCCATGAT CAACGGCTGG 
GGCTCAGCCT CAGAGGAGGA CAACATTTCC AGCGGACGCT CCAGTGTTAG TTCTTCGGAT 
GGCTCCTTTT TCACTGATGC TGACTTTGCC CAGGCAGTCG CAGCAGCGGC AGAGTATGCT 
GGTCTGAAAG TGGCACGACG GCAAATGCAG GATGCTGCTG GCCGTCGACA TTTTCATGCG 
TCTCAGTGCC CTAGGCCCAC AAGTCCTGTG TCTACAGACA GCAACATGAG TGCCGCCGTA 
ATGCAGAAAA CCAGACCAGC CAAGAAACCG AAACACCAGC CAGGACATCT GCGCAGAGAA 
ACCTACACAG ATGATCTTCC ACCACCTCCT GTGCCGCCAC CTGCTATAAA GTCACCTACT 
GCCCAATCCA AGACACAGCT GGAAGTACGA CCTGTAGTGG TGCCAAAACT CCCTTCTGTG 
GATGCAAGAA CAGACAGATC ATCAGACAGA AAAGGAAGCA GTTACAAGGG GAGAGAAGTA 
TTGGATGGAA GACAGGTTGT TGACATGCGA ACAAATCCAG GTGATCCCAG AGAAGCACAG 
GAACAGCAAA ATGACGGGAA AGGACGTGGA AACAAGGCAG CAAAACGAGA CCTTCCACCA 
GCAAAGACTC ATCTCATCCA AGAGGATATT CTACCTTATT GTAGACCTAC TTTTCCAACA 
TCAAATAATC CCAGAGATCC CAGTTCCTCA AGCTCAATGT CATCAAGAGG ATCAGGAAGC 
AGACAAAGAG AACAAGCAAA TGTAGGTCGA AGAAATATTG CAGAAATGCA GGTACTTGGA 
GGATATGAAA GAGGAGAAGA TAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796991.1
CDS	36..4949
Translation	MAVLLLMLLLSMAEITSPVGVLDRTKAGSRWKGEKNRSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941502.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941502.2

ORF Insert Sequence:

ATGGCTGTGC TGTTACTGAT GCTGCTGCTC TCGATGGCTG AGATAACTTC TCCTGTGGGT 
GTCTTGGACA GAACGAAAGC TGGGTCAAGG TGGAAAGGTG AAAAGAATCG CTCCCGTCTT 
CGTCAGGAAG ATTTTCCACC TCGCATTGTT GAACACCCTT CAGACCTGAT TGTCTCAAAA 
GGAGAACCTG CAACTTTGAA CTGCAAAGCT GAAGGCCGCC CCACACCCAC TATTGAATGG 
TACAAAGGGG GAGAGAGAGT GGAGACAGAC AAAGATGACC CTCGCTCACA CCGAATGTTG 
CTGCCGAGTG GATCTTTATT TTTCTTACGT ATAGTACATG GACGGAAAAG TAGACCTGAT 
GAAGGAGTCT ATGTCTGTGT AGCAAGGAAT TACCTTGGAG AGGCTGTGAG CCACAATGCA 
TCGCTGGAAG TAGCCATACT TCGGGATGAC TTCAGACAAA ACCCTTCGGA TGTCATGGTT 
GCAGTAGGAG AGCCTGCAGT AATGGAATGC CAACCTCCAC GAGGCCATCC TGAGCCCACC 
ATTTCATGGA AGAAAGATGG CTCTCCACTG GATGATAAAG ATGAAAGAAT AACTATACGA 
GGAGGAAAGC TCATGATCAC TTACACCCGT AAAAGTGACG CTGGCAAATA TGTTTGTGTT 
GGTACCAATA TGGTTGGGGA ACGTGAGAGT GAAGTAGCCG AGCTGACTGT CTTAGAGAGA 
CCATCATTTG TGAAGAGACC CAGTAACTTG GCAGTAACTG TGGATGACAG TGCAGAATTT 
AAATGTGAGG CCCGAGGTGA CCCTGTACCT ACAGTACGAT GGAGGAAAGA TGATGGAGAG 
CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACCT TGAAAATTAG GAAGGTGACA 
GCTGGTGACA TGGGTTCATA CACTTGTGTT GCAGAAAATA TGGTGGGCAA AGCTGAAGCA 
TCTGCTACTC TGACTGTGCA AGTTGAGTCT GAACCTCCAC ATTTTGTTGT GAAACCCCGT 
GACCAGGTTG TTGCTTTGGG ACGGACTGTA ACTTTTCAGT GTGAAGCAAC CGGAAATCCT 
CAACCAGCTA TTTTTTGGAG GAGAGAAGGG AGTCAGAATC TACTTTTCTC ATATCAACCA 
CCACAGTCAT CCAGCCGATT TTCAGTCTCC CAGACTGGCG ACCTCACAAT TACTAATGTC 
CAGCGATCTG ATGTTGGTTA TTACATCTGC CAGACTTTAA ATGTTGCTGG AAGCATCATC 
ACAAAGGCAT ATTTGGAAGT TACAGATGTG ATTGCAGATC GGCCTCCCCC AGTTATTCGA 
CAAGGTCCTG TGAATCAGAC TGTAGCTGTG GATGGCACTT TCGTCCTCAG CTGTGTGGCC 
ACAGGCAGTC CAGTGCCCAC CATTCTGTGG AGAAAGGATG GAGTCCTCGT TTCAACCCAA 
GACTCTCGAA TCAAACAGTT GGAGAATGGA GTACTGCAGA TCCGATATGC TAAGCTGGGT 
GATACTGGTC GGTACACCTG CATTGCATCA ACCCCCAGTG GTGAAGCAAC ATGGAGTGCT 
TACATTGAAG TTCAAGAATT TGGAGTTCCA GTTCAGCCTC CAAGACCTAC TGACCCAAAT 
TTAATCCCTA GTGCCCCATC AAAACCTGAA GTGACAGATG TCAGCAGAAA TACAGTCACA 
TTATCATGGC AACCAAATTT GAATTCAGGA GCAACTCCAA CATCTTATAT TATAGAAGCC 
TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACCGTAGCAG AGAATGTGAA AACAGAAACA 
TTTGCCATTA AAGGACTCAA ACCTAATGCA ATTTACCTTT TCCTTGTGAG GGCAGCTAAT 
GCATATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACACA AGATGTCCTA 
CCAACAAGTC AGGGGGTGGA CCACAAGCAG GTCCAGAGAG AGCTGGGGAA TGCTGTTCTG 
CACCTCCACA ACCCCACCGT CCTTTCTTCC TCTTCCATCG AAGTGCACTG GACAGTAGAT 
CAACAGTCTC AGTATATACA AGGATATAAA ATTCTCTATC GGCCATCTGG AGCCAACCAC 
GGAGAATCAG ACTGGTTAGT TTTTGAAGTG AGGACGCCAG CCAAAAACAG TGTGGTTATC 
CCTGATCTCA GAAAGGGAGT CAACTATGAA ATTAAGGCTC GCCCTTTTTT TAATGAATTT 
CAAGGAGCAG ATAGTGAAAT CAAGTTCGCC AAAACCCTGG AAGAAGCACC CAGTGCCCCA 
CCCCAAGGTG TAACTGTATC CAAGAATGAT GGAAACGGAA CTGCAATTCT AGTTAGTTGG 
CAGCCACCTC CAGAAGACAC TCAAAATGGA ATGGTCCAAG AGTATAAGGT TTGGTGTCTG 
GGCAATGAAA CTCGATACCA CATCAACAAA ACAGTGGATG GTTCCACCTT TTCCGTGGTC 
ATTCCCTTTC TTGTTCCTGG AATCCGATAC AGTGTGGAAG TGGCAGCCAG CACTGGGGCT 
GGGTCTGGGG TAAAGAGTGA GCCTCAGTTC ATCCAGCTGG ATGCCCATGG AAACCCTGTG 
TCACCTGAGG ACCAAGTCAG CCTCGCTCAG CAGATTTCAG ATGTGGTGAA GCAGCCGGCC 
TTCATAGCAG GTATTGGAGC AGCCTGTTGG ATCATCCTCA TGGTCTTCAG CATCTGGCTT 
TATCGACACC GCAAGAAGAG AAACGGACTT ACTAGTACTT ACGCGGGTAT CAGAAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTAACT TACCAGAGAG GAGGCGAAGC TGTCAGCAGT 
GGAGGGAGGC CCGGACTTCT CAACATCAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC 
ACGTGGCCTA ATACTGGCAA CAACCACAAT GACTGCTCCA TCAGCTGCTG CACGGCAGGC 
AATGGAAACA GCGACAGCAA CCTCACTACC TACAGTCGCC CAGCTGATTG TATAGCAAAT 
TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT 
GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG 
AAGGATGGGC GTTTTGTCAA TCCATCAGGG CAGCCTACTC CTTATGCCAC CACTCAGCTC 
ATCCAGTCAA ACCTCAGCAA CAACATGAAC AATGGCAGCG GGGACTCTGG CGAGAAGCAC 
TGGAAACCAC TGGGACAGCA GAAACAAGAA GTGGCACCAG TTCAGTACAA CATCATGGAG 
CAAAACAAGC TGAACAAAGG TGAAGAGGCT TATTATCGAG CAAATGACAC AGTTCCTCAA 
ACTATCCCAT ACAACCAATC ATACGACCAG AACACAGGAG GATCCTACAA CAGCTCAGAC 
CGGGGCAGTA GTACATCTGG GAGTCAGGGG CACAAGAAAG GGGCAAGAAC ACCCAAGGTA 
CCAAAACAGG GTGGCATGAA CTGGGCAGAC CTGCTTCCTC CTCCCCCAGC ACATCCTCCT 
CCACACAGCA ATAGCGAAGA GTACAACATT TCTGTAGATG AAAGCTATGA CCAAGAAATG 
CCATGTCCCG TGCCACCAGC AAGGATGTAT TTGCAACAAG ATGAATTAGA AGAGGAGGAA 
GATGAACGAG GCCCCACTCC CCCCGTTCGG GGAGCAGCTT CTTCTCCAGC TGCCGTGTCC 
TATAGCCATC AGTCCACTGC CACTCTGACT CCCTCCCCAC AGGAAGAACT CCAGCCCATG 
TTACAGGATT GTCCAGAGGA GACTGGCCAC ATGCAGCACC AGCCCGACAG GAGACGGCAG 
CCTGTGAGTC CTCCTCCACC ACCACGGCCG ATCTCCCCTC CACATACCTA TGGCTACATT 
TCAGGACCCC TGGTCTCAGA TATGGATACA GATGCGCCAG AAGAGGAAGA AGACGAAGCC 
GACATGGAGG TAGCCAAGAT GCAAACCAGA AGGCTTTTGT TACGTGGGCT TGAGCAGACA 
CCTGCCTCCA GTGTTGGGGA CCTGGAGAGC TCTGTCACGG GGTCCATGAT CAACGGCTGG 
GGCTCAGCCT CAGAGGAGGA CAACATTTCC AGCGGACGCT CCAGTGTTAG TTCTTCGGAT 
GGCTCCTTTT TCACTGATGC TGACTTTGCC CAGGCAGTCG CAGCAGCGGC AGAGTATGCT 
GGTCTGAAAG TGGCACGACG GCAAATGCAG GATGCTGCTG GCCGTCGACA TTTTCATGCG 
TCTCAGTGCC CTAGGCCCAC AAGTCCTGTG TCTACAGACA GCAACATGAG TGCCGCCGTA 
ATGCAGAAAA CCAGACCAGC CAAGAAACCG AAACACCAGC CAGGACATCT GCGCAGAGAA 
ACCTACACAG ATGATCTTCC ACCACCTCCT GTGCCGCCAC CTGCTATAAA GTCACCTACT 
GCCCAATCCA AGACACAGCT GGAAGTACGA CCTGTAGTGG TGCCAAAACT CCCTTCTGTG 
GATGCAAGAA CAGACAGATC ATCAGACAGA AAAGGAAGCA GTTACAAGGG GAGAGAAGTA 
TTGGATGGAA GACAGGTTGT TGACATGCGA ACAAATCCAG GTGATCCCAG AGAAGCACAG 
GAACAGCAAA ATGACGGGAA AGGACGTGGA AACAAGGCAG CAAAACGAGA CCTTCCACCA 
GCAAAGACTC ATCTCATCCA AGAGGATATT CTACCTTATT GTAGACCTAC TTTTCCAACA 
TCAAATAATC CCAGAGATCC CAGTTCCTCA AGCTCAATGT CATCAAGAGG ATCAGGAAGC 
AGACAAAGAG AACAAGCAAA TGTAGGTCGA AGAAATATTG CAGAAATGCA GGTACTTGGA 
GGATATGAAA GAGGAGAAGA TAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_024355572.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211340.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OPc105018
Clone ID Related Accession (Same CDS sequence)	XM_024355572.1
Accession Version	XM_024355572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TCCCGTCTTT TACCTTCACA 
CCAACAGTAA CTTACCAGAG AGGAGGCGAA GCTGTCAGCA GTGGAGGGAG GCCCGGACTT 
CTCAACATCA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC TAATACTGGC 
AACAACCACA ATGACTGCTC CATCAGCTGC TGCACGGCAG GCAATGGAAA CAGCGACAGC 
AACCTCACTA CCTACAGTCG CCCAGGGCAG CCTACTCCTT ATGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGGTGA AGAGGCTTAT TATCGAGCAA ATGACACAGT TCCTCAAACT 
ATCCCATACA ACCAATCATA CGACCAGAAC ACAGGAGGAT CCTACAACAG CTCAGACCGG 
GGCAGTAGTA CATCTGGGAG TCAGGGGCAC AAGAAAGGGG CAAGAACACC CAAGGTACCA 
AAACAGGGTG GCATGAACTG GGCAGACCTG CTTCCTCCTC CCCCAGCACA TCCTCCTCCA 
CACAGCAATA GCGAAGAGTA CAACATTTCT GTAGATGAAA GCTATGACCA AGAAATGCCA 
TGTCCCGTGC CACCAGCAAG GATGTATTTG CAACAAGATG AATTAGAAGA GGAGGAAGAT 
GAACGAGGCC CCACTCCCCC CGTTCGGGGA GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT 
AGCCATCAGT CCACTGCCAC TCTGACTCCC TCCCCACAGG AAGAACTCCA GCCCATGTTA 
CAGGATTGTC CAGAGGAGAC TGGCCACATG CAGCACCAGC CCGACAGGAG ACGGCAGCCT 
GTGAGTCCTC CTCCACCACC ACGGCCGATC TCCCCTCCAC ATACCTATGG CTACATTTCA 
GGACCCCTGG TCTCAGATAT GGATACAGAT GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC 
ATGGAGGTAG CCAAGATGCA AACCAGAAGG CTTTTGTTAC GTGGGCTTGA GCAGACACCT 
GCCTCCAGTG TTGGGGACCT GGAGAGCTCT GTCACGGGGT CCATGATCAA CGGCTGGGGC 
TCAGCCTCAG AGGAGGACAA CATTTCCAGC GGACGCTCCA GTGTTAGTTC TTCGGATGGC 
TCCTTTTTCA CTGATGCTGA CTTTGCCCAG GCAGTCGCAG CAGCGGCAGA GTATGCTGGT 
CTGAAAGTGG CACGACGGCA AATGCAGGAT GCTGCTGGCC GTCGACATTT TCATGCGTCT 
CAGTGCCCTA GGCCCACAAG TCCTGTGTCT ACAGACAGCA ACATGAGTGC CGCCGTAATG 
CAGAAAACCA GACCAGCCAA GAAACCGAAA CACCAGCCAG GACATCTGCG CAGAGAAACC 
TACACAGATG ATCTTCCACC ACCTCCTGTG CCGCCACCTG CTATAAAGTC ACCTACTGCC 
CAATCCAAGA CACAGCTGGA AGTACGACCT GTAGTGGTGC CAAAACTCCC TTCTGTGGAT 
GCAAGAACAG ACAGATCATC AGACAGAAAA GGAAGCAGTT ACAAGGGGAG AGAAGTATTG 
GATGGAAGAC AGGTTGTTGA CATGCGAACA AATCCAGGTG ATCCCAGAGA AGCACAGGAA 
CAGCAAAATG ACGGGAAAGG ACGTGGAAAC AAGGCAGCAA AACGAGACCT TCCACCAGCA 
AAGACTCATC TCATCCAAGA GGATATTCTA CCTTATTGTA GACCTACTTT TCCAACATCA 
AATAATCCCA GAGATCCCAG TTCCTCAAGC TCAATGTCAT CAAGAGGATC AGGAAGCAGA 
CAAAGAGAAC AAGCAAATGT AGGTCGAAGA AATATTGCAG AAATGCAGGT ACTTGGAGGA 
TATGAAAGAG GAGAAGATAA TAATGAAGAA TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211340.1
CDS	56..4966
Translation	MMLRPSQGLVCRRRGKDEACCVLSLCVFLSHVDLLVSGHAVIEMATTVTTVTPSAALVLFPTDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_024355572.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024355572.1

ORF Insert Sequence:

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TCCCGTCTTT TACCTTCACA 
CCAACAGTAA CTTACCAGAG AGGAGGCGAA GCTGTCAGCA GTGGAGGGAG GCCCGGACTT 
CTCAACATCA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC TAATACTGGC 
AACAACCACA ATGACTGCTC CATCAGCTGC TGCACGGCAG GCAATGGAAA CAGCGACAGC 
AACCTCACTA CCTACAGTCG CCCAGGGCAG CCTACTCCTT ATGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGGTGA AGAGGCTTAT TATCGAGCAA ATGACACAGT TCCTCAAACT 
ATCCCATACA ACCAATCATA CGACCAGAAC ACAGGAGGAT CCTACAACAG CTCAGACCGG 
GGCAGTAGTA CATCTGGGAG TCAGGGGCAC AAGAAAGGGG CAAGAACACC CAAGGTACCA 
AAACAGGGTG GCATGAACTG GGCAGACCTG CTTCCTCCTC CCCCAGCACA TCCTCCTCCA 
CACAGCAATA GCGAAGAGTA CAACATTTCT GTAGATGAAA GCTATGACCA AGAAATGCCA 
TGTCCCGTGC CACCAGCAAG GATGTATTTG CAACAAGATG AATTAGAAGA GGAGGAAGAT 
GAACGAGGCC CCACTCCCCC CGTTCGGGGA GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT 
AGCCATCAGT CCACTGCCAC TCTGACTCCC TCCCCACAGG AAGAACTCCA GCCCATGTTA 
CAGGATTGTC CAGAGGAGAC TGGCCACATG CAGCACCAGC CCGACAGGAG ACGGCAGCCT 
GTGAGTCCTC CTCCACCACC ACGGCCGATC TCCCCTCCAC ATACCTATGG CTACATTTCA 
GGACCCCTGG TCTCAGATAT GGATACAGAT GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC 
ATGGAGGTAG CCAAGATGCA AACCAGAAGG CTTTTGTTAC GTGGGCTTGA GCAGACACCT 
GCCTCCAGTG TTGGGGACCT GGAGAGCTCT GTCACGGGGT CCATGATCAA CGGCTGGGGC 
TCAGCCTCAG AGGAGGACAA CATTTCCAGC GGACGCTCCA GTGTTAGTTC TTCGGATGGC 
TCCTTTTTCA CTGATGCTGA CTTTGCCCAG GCAGTCGCAG CAGCGGCAGA GTATGCTGGT 
CTGAAAGTGG CACGACGGCA AATGCAGGAT GCTGCTGGCC GTCGACATTT TCATGCGTCT 
CAGTGCCCTA GGCCCACAAG TCCTGTGTCT ACAGACAGCA ACATGAGTGC CGCCGTAATG 
CAGAAAACCA GACCAGCCAA GAAACCGAAA CACCAGCCAG GACATCTGCG CAGAGAAACC 
TACACAGATG ATCTTCCACC ACCTCCTGTG CCGCCACCTG CTATAAAGTC ACCTACTGCC 
CAATCCAAGA CACAGCTGGA AGTACGACCT GTAGTGGTGC CAAAACTCCC TTCTGTGGAT 
GCAAGAACAG ACAGATCATC AGACAGAAAA GGAAGCAGTT ACAAGGGGAG AGAAGTATTG 
GATGGAAGAC AGGTTGTTGA CATGCGAACA AATCCAGGTG ATCCCAGAGA AGCACAGGAA 
CAGCAAAATG ACGGGAAAGG ACGTGGAAAC AAGGCAGCAA AACGAGACCT TCCACCAGCA 
AAGACTCATC TCATCCAAGA GGATATTCTA CCTTATTGTA GACCTACTTT TCCAACATCA 
AATAATCCCA GAGATCCCAG TTCCTCAAGC TCAATGTCAT CAAGAGGATC AGGAAGCAGA 
CAAAGAGAAC AAGCAAATGT AGGTCGAAGA AATATTGCAG AAATGCAGGT ACTTGGAGGA 
TATGAAAGAG GAGAAGATAA TAATGAAGAA TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_024355573.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024211341.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X11, mRNA.

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CloneID	OPc105019
Clone ID Related Accession (Same CDS sequence)	XM_024355573.1
Accession Version	XM_024355573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TAACTTACCA GAGAGGAGGC 
GAAGCTGTCA GCAGTGGAGG GAGGCCCGGA CTTCTCAACA TCAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCTAATACT GGCAACAACC ACAATGACTG CTCCATCAGC 
TGCTGCACGG CAGGCAATGG AAACAGCGAC AGCAACCTCA CTACCTACAG TCGCCCAGGG 
CAGCCTACTC CTTATGCCAC CACTCAGCTC ATCCAGTCAA ACCTCAGCAA CAACATGAAC 
AATGGCAGCG GGGACTCTGG CGAGAAGCAC TGGAAACCAC TGGGACAGCA GAAACAAGAA 
GTGGCACCAG TTCAGTACAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGAGCA 
AATGACACAG TTCCTCAAAC TATCCCATAC AACCAATCAT ACGACCAGAA CACAGGAGGA 
TCCTACAACA GCTCAGACCG GGGCAGTAGT ACATCTGGGA GTCAGGGGCA CAAGAAAGGG 
GCAAGAACAC CCAAGGTACC AAAACAGGGT GGCATGAACT GGGCAGACCT GCTTCCTCCT 
CCCCCAGCAC ATCCTCCTCC ACACAGCAAT AGCGAAGAGT ACAACATTTC TGTAGATGAA 
AGCTATGACC AAGAAATGCC ATGTCCCGTG CCACCAGCAA GGATGTATTT GCAACAAGAT 
GAATTAGAAG AGGAGGAAGA TGAACGAGGC CCCACTCCCC CCGTTCGGGG AGCAGCTTCT 
TCTCCAGCTG CCGTGTCCTA TAGCCATCAG TCCACTGCCA CTCTGACTCC CTCCCCACAG 
GAAGAACTCC AGCCCATGTT ACAGGATTGT CCAGAGGAGA CTGGCCACAT GCAGCACCAG 
CCCGACAGGA GACGGCAGCC TGTGAGTCCT CCTCCACCAC CACGGCCGAT CTCCCCTCCA 
CATACCTATG GCTACATTTC AGGACCCCTG GTCTCAGATA TGGATACAGA TGCGCCAGAA 
GAGGAAGAAG ACGAAGCCGA CATGGAGGTA GCCAAGATGC AAACCAGAAG GCTTTTGTTA 
CGTGGGCTTG AGCAGACACC TGCCTCCAGT GTTGGGGACC TGGAGAGCTC TGTCACGGGG 
TCCATGATCA ACGGCTGGGG CTCAGCCTCA GAGGAGGACA ACATTTCCAG CGGACGCTCC 
AGTGTTAGTT CTTCGGATGG CTCCTTTTTC ACTGATGCTG ACTTTGCCCA GGCAGTCGCA 
GCAGCGGCAG AGTATGCTGG TCTGAAAGTG GCACGACGGC AAATGCAGGA TGCTGCTGGC 
CGTCGACATT TTCATGCGTC TCAGTGCCCT AGGCCCACAA GTCCTGTGTC TACAGACAGC 
AACATGAGTG CCGCCGTAAT GCAGAAAACC AGACCAGCCA AGAAACCGAA ACACCAGCCA 
GGACATCTGC GCAGAGAAAC CTACACAGAT GATCTTCCAC CACCTCCTGT GCCGCCACCT 
GCTATAAAGT CACCTACTGC CCAATCCAAG ACACAGCTGG AAGTACGACC TGTAGTGGTG 
CCAAAACTCC CTTCTGTGGA TGCAAGAACA GACAGATCAT CAGACAGAAA AGGAAGCAGT 
TACAAGGGGA GAGAAGTATT GGATGGAAGA CAGGTTGTTG ACATGCGAAC AAATCCAGGT 
GATCCCAGAG AAGCACAGGA ACAGCAAAAT GACGGGAAAG GACGTGGAAA CAAGGCAGCA 
AAACGAGACC TTCCACCAGC AAAGACTCAT CTCATCCAAG AGGATATTCT ACCTTATTGT 
AGACCTACTT TTCCAACATC AAATAATCCC AGAGATCCCA GTTCCTCAAG CTCAATGTCA 
TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA CAAGCAAATG TAGGTCGAAG AAATATTGCA 
GAAATGCAGG TACTTGGAGG ATATGAAAGA GGAGAAGATA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024211341.1
CDS	54..4925
Translation	MMLRPSQGLVCRRRGKDEACCVLSLCVFLSHVDLLVSGHAVIEMATTVTTVTPSAALVLFPTDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_024355573.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024355573.1

ORF Insert Sequence:

ATGATGCTGA GACCTTCGCA GGGCTTAGTG TGCCGCAGGA GGGGCAAGGA TGAAGCGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTACT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAAGATGT AGAGAGGGGG AACGACCACG GGACGCCAAT CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTATACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCTCGCATTG TTGAACACCC TTCAGACCTG ATTGTCTCAA AAGGAGAACC TGCAACTTTG 
AACTGCAAAG CTGAAGGCCG CCCCACACCC ACTATTGAAT GGTACAAAGG GGGAGAGAGA 
GTGGAGACAG ACAAAGATGA CCCTCGCTCA CACCGAATGT TGCTGCCGAG TGGATCTTTA 
TTTTTCTTAC GTATAGTACA TGGACGGAAA AGTAGACCTG ATGAAGGAGT CTATGTCTGT 
GTAGCAAGGA ATTACCTTGG AGAGGCTGTG AGCCACAATG CATCGCTGGA AGTAGCCATA 
CTTCGGGATG ACTTCAGACA AAACCCTTCG GATGTCATGG TTGCAGTAGG AGAGCCTGCA 
GTAATGGAAT GCCAACCTCC ACGAGGCCAT CCTGAGCCCA CCATTTCATG GAAGAAAGAT 
GGCTCTCCAC TGGATGATAA AGATGAAAGA ATAACTATAC GAGGAGGAAA GCTCATGATC 
ACTTACACCC GTAAAAGTGA CGCTGGCAAA TATGTTTGTG TTGGTACCAA TATGGTTGGG 
GAACGTGAGA GTGAAGTAGC CGAGCTGACT GTCTTAGAGA GACCATCATT TGTGAAGAGA 
CCCAGTAACT TGGCAGTAAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGT 
GACCCTGTAC CTACAGTACG ATGGAGGAAA GATGATGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC CTTGAAAATT AGGAAGGTGA CAGCTGGTGA CATGGGTTCA 
TACACTTGTG TTGCAGAAAA TATGGTGGGC AAAGCTGAAG CATCTGCTAC TCTGACTGTG 
CAAGTTGAGT CTGAACCTCC ACATTTTGTT GTGAAACCCC GTGACCAGGT TGTTGCTTTG 
GGACGGACTG TAACTTTTCA GTGTGAAGCA ACCGGAAATC CTCAACCAGC TATTTTTTGG 
AGGAGAGAAG GGAGTCAGAA TCTACTTTTC TCATATCAAC CACCACAGTC ATCCAGCCGA 
TTTTCAGTCT CCCAGACTGG CGACCTCACA ATTACTAATG TCCAGCGATC TGATGTTGGT 
TATTACATCT GCCAGACTTT AAATGTTGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA 
GTTACAGATG TGATTGCAGA TCGGCCTCCC CCAGTTATTC GACAAGGTCC TGTGAATCAG 
ACTGTAGCTG TGGATGGCAC TTTCGTCCTC AGCTGTGTGG CCACAGGCAG TCCAGTGCCC 
ACCATTCTGT GGAGAAAGGA TGGAGTCCTC GTTTCAACCC AAGACTCTCG AATCAAACAG 
TTGGAGAATG GAGTACTGCA GATCCGATAT GCTAAGCTGG GTGATACTGG TCGGTACACC 
TGCATTGCAT CAACCCCCAG TGGTGAAGCA ACATGGAGTG CTTACATTGA AGTTCAAGAA 
TTTGGAGTTC CAGTTCAGCC TCCAAGACCT ACTGACCCAA ATTTAATCCC TAGTGCCCCA 
TCAAAACCTG AAGTGACAGA TGTCAGCAGA AATACAGTCA CATTATCATG GCAACCAAAT 
TTGAATTCAG GAGCAACTCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACCGTAGC AGAGAATGTG AAAACAGAAA CATTTGCCAT TAAAGGACTC 
AAACCTAATG CAATTTACCT TTTCCTTGTG AGGGCAGCTA ATGCATATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACA CAAGATGTCC TACCAACAAG TCAGGGGGTG 
GACCACAAGC AGGTCCAGAG AGAGCTGGGG AATGCTGTTC TGCACCTCCA CAACCCCACC 
GTCCTTTCTT CCTCTTCCAT CGAAGTGCAC TGGACAGTAG ATCAACAGTC TCAGTATATA 
CAAGGATATA AAATTCTCTA TCGGCCATCT GGAGCCAACC ACGGAGAATC AGACTGGTTA 
GTTTTTGAAG TGAGGACGCC AGCCAAAAAC AGTGTGGTTA TCCCTGATCT CAGAAAGGGA 
GTCAACTATG AAATTAAGGC TCGCCCTTTT TTTAATGAAT TTCAAGGAGC AGATAGTGAA 
ATCAAGTTCG CCAAAACCCT GGAAGAAGCA CCCAGTGCCC CACCCCAAGG TGTAACTGTA 
TCCAAGAATG ATGGAAACGG AACTGCAATT CTAGTTAGTT GGCAGCCACC TCCAGAAGAC 
ACTCAAAATG GAATGGTCCA AGAGTATAAG GTTTGGTGTC TGGGCAATGA AACTCGATAC 
CACATCAACA AAACAGTGGA TGGTTCCACC TTTTCCGTGG TCATTCCCTT TCTTGTTCCT 
GGAATCCGAT ACAGTGTGGA AGTGGCAGCC AGCACTGGGG CTGGGTCTGG GGTAAAGAGT 
GAGCCTCAGT TCATCCAGCT GGATGCCCAT GGAAACCCTG TGTCACCTGA GGACCAAGTC 
AGCCTCGCTC AGCAGATTTC AGATGTGGTG AAGCAGCCGG CCTTCATAGC AGGTATTGGA 
GCAGCCTGTT GGATCATCCT CATGGTCTTC AGCATCTGGC TTTATCGACA CCGCAAGAAG 
AGAAACGGAC TTACTAGTAC TTACGCGGGT ATCAGAAAAG TAACTTACCA GAGAGGAGGC 
GAAGCTGTCA GCAGTGGAGG GAGGCCCGGA CTTCTCAACA TCAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCTAATACT GGCAACAACC ACAATGACTG CTCCATCAGC 
TGCTGCACGG CAGGCAATGG AAACAGCGAC AGCAACCTCA CTACCTACAG TCGCCCAGGG 
CAGCCTACTC CTTATGCCAC CACTCAGCTC ATCCAGTCAA ACCTCAGCAA CAACATGAAC 
AATGGCAGCG GGGACTCTGG CGAGAAGCAC TGGAAACCAC TGGGACAGCA GAAACAAGAA 
GTGGCACCAG TTCAGTACAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGAGCA 
AATGACACAG TTCCTCAAAC TATCCCATAC AACCAATCAT ACGACCAGAA CACAGGAGGA 
TCCTACAACA GCTCAGACCG GGGCAGTAGT ACATCTGGGA GTCAGGGGCA CAAGAAAGGG 
GCAAGAACAC CCAAGGTACC AAAACAGGGT GGCATGAACT GGGCAGACCT GCTTCCTCCT 
CCCCCAGCAC ATCCTCCTCC ACACAGCAAT AGCGAAGAGT ACAACATTTC TGTAGATGAA 
AGCTATGACC AAGAAATGCC ATGTCCCGTG CCACCAGCAA GGATGTATTT GCAACAAGAT 
GAATTAGAAG AGGAGGAAGA TGAACGAGGC CCCACTCCCC CCGTTCGGGG AGCAGCTTCT 
TCTCCAGCTG CCGTGTCCTA TAGCCATCAG TCCACTGCCA CTCTGACTCC CTCCCCACAG 
GAAGAACTCC AGCCCATGTT ACAGGATTGT CCAGAGGAGA CTGGCCACAT GCAGCACCAG 
CCCGACAGGA GACGGCAGCC TGTGAGTCCT CCTCCACCAC CACGGCCGAT CTCCCCTCCA 
CATACCTATG GCTACATTTC AGGACCCCTG GTCTCAGATA TGGATACAGA TGCGCCAGAA 
GAGGAAGAAG ACGAAGCCGA CATGGAGGTA GCCAAGATGC AAACCAGAAG GCTTTTGTTA 
CGTGGGCTTG AGCAGACACC TGCCTCCAGT GTTGGGGACC TGGAGAGCTC TGTCACGGGG 
TCCATGATCA ACGGCTGGGG CTCAGCCTCA GAGGAGGACA ACATTTCCAG CGGACGCTCC 
AGTGTTAGTT CTTCGGATGG CTCCTTTTTC ACTGATGCTG ACTTTGCCCA GGCAGTCGCA 
GCAGCGGCAG AGTATGCTGG TCTGAAAGTG GCACGACGGC AAATGCAGGA TGCTGCTGGC 
CGTCGACATT TTCATGCGTC TCAGTGCCCT AGGCCCACAA GTCCTGTGTC TACAGACAGC 
AACATGAGTG CCGCCGTAAT GCAGAAAACC AGACCAGCCA AGAAACCGAA ACACCAGCCA 
GGACATCTGC GCAGAGAAAC CTACACAGAT GATCTTCCAC CACCTCCTGT GCCGCCACCT 
GCTATAAAGT CACCTACTGC CCAATCCAAG ACACAGCTGG AAGTACGACC TGTAGTGGTG 
CCAAAACTCC CTTCTGTGGA TGCAAGAACA GACAGATCAT CAGACAGAAA AGGAAGCAGT 
TACAAGGGGA GAGAAGTATT GGATGGAAGA CAGGTTGTTG ACATGCGAAC AAATCCAGGT 
GATCCCAGAG AAGCACAGGA ACAGCAAAAT GACGGGAAAG GACGTGGAAA CAAGGCAGCA 
AAACGAGACC TTCCACCAGC AAAGACTCAT CTCATCCAAG AGGATATTCT ACCTTATTGT 
AGACCTACTT TTCCAACATC AAATAATCCC AGAGATCCCA GTTCCTCAAG CTCAATGTCA 
TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA CAAGCAAATG TAGGTCGAAG AAATATTGCA 
GAAATGCAGG TACTTGGAGG ATATGAAAGA GGAGAAGATA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941503.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796992.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X12, mRNA.

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CloneID	OPc105020
Clone ID Related Accession (Same CDS sequence)	XM_016941503.2
Accession Version	XM_016941503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016941503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGGAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC ATCAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA TGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA GAGGCTTATT ATCGAGCAAA TGACACAGTT 
CCTCAAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCC GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACAGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGATGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTGGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCTGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACCGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTGTGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796992.1
CDS	1203..6062
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941503.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941503.2

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGGAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC ATCAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA TGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA GAGGCTTATT ATCGAGCAAA TGACACAGTT 
CCTCAAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCC GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACAGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGATGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTGGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCTGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACCGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTGTGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941504.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796993.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X13, mRNA.

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CloneID	OPc105021
Clone ID Related Accession (Same CDS sequence)	XM_016941504.2
Accession Version	XM_016941504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016941504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGACG GACTGTAACT TTTCAGTGTG AAGCAACCGG AAATCCTCAA 
CCAGCTATTT TTTGGAGGAG AGAAGGGAGT CAGAATCTAC TTTTCTCATA TCAACCACCA 
CAGTCATCCA GCCGATTTTC AGTCTCCCAG ACTGGCGACC TCACAATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA CATCTGCCAG ACTTTAAATG TTGCTGGAAG CATCATCACA 
AAGGCATATT TGGAAGTTAC AGATGTGATT GCAGATCGGC CTCCCCCAGT TATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCTGTGGAT GGCACTTTCG TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACCAT TCTGTGGAGA AAGGATGGAG TCCTCGTTTC AACCCAAGAC 
TCTCGAATCA AACAGTTGGA GAATGGAGTA CTGCAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AAGCAACATG GAGTGCTTAC 
ATTGAAGTTC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCTACTGA CCCAAATTTA 
ATCCCTAGTG CCCCATCAAA ACCTGAAGTG ACAGATGTCA GCAGAAATAC AGTCACATTA 
TCATGGCAAC CAAATTTGAA TTCAGGAGCA ACTCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC AGAAACATTT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCTAATGCA 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACACAAGA TGTCCTACCA 
ACAAGTCAGG GGGTGGACCA CAAGCAGGTC CAGAGAGAGC TGGGGAATGC TGTTCTGCAC 
CTCCACAACC CCACCGTCCT TTCTTCCTCT TCCATCGAAG TGCACTGGAC AGTAGATCAA 
CAGTCTCAGT ATATACAAGG ATATAAAATT CTCTATCGGC CATCTGGAGC CAACCACGGA 
GAATCAGACT GGTTAGTTTT TGAAGTGAGG ACGCCAGCCA AAAACAGTGT GGTTATCCCT 
GATCTCAGAA AGGGAGTCAA CTATGAAATT AAGGCTCGCC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATCAA GTTCGCCAAA ACCCTGGAAG AAGCACCCAG TGCCCCACCC 
CAAGGTGTAA CTGTATCCAA GAATGATGGA AACGGAACTG CAATTCTAGT TAGTTGGCAG 
CCACCTCCAG AAGACACTCA AAATGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTC GATACCACAT CAACAAAACA GTGGATGGTT CCACCTTTTC CGTGGTCATT 
CCCTTTCTTG TTCCTGGAAT CCGATACAGT GTGGAAGTGG CAGCCAGCAC TGGGGCTGGG 
TCTGGGGTAA AGAGTGAGCC TCAGTTCATC CAGCTGGATG CCCATGGAAA CCCTGTGTCA 
CCTGAGGACC AAGTCAGCCT CGCTCAGCAG ATTTCAGATG TGGTGAAGCA GCCGGCCTTC 
ATAGCAGGTA TTGGAGCAGC CTGTTGGATC ATCCTCATGG TCTTCAGCAT CTGGCTTTAT 
CGACACCGCA AGAAGAGAAA CGGACTTACT AGTACTTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAC CAGAGAGGAG GCGAAGCTGT CAGCAGTGGA 
GGGAGGCCCG GACTTCTCAA CATCAGTGAA CCTGCCACGC AGCCATGGCT GGCAGACACG 
TGGCCTAATA CTGGCAACAA CCACAATGAC TGCTCCATCA GCTGCTGCAC GGCAGGCAAT 
GGAAACAGCG ACAGCAACCT CACTACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCTACTCCTT ATGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGAGCAAAT GACACAGTTC CTCAAACTAT CCCATACAAC 
CAATCATACG ACCAGAACAC AGGAGGATCC TACAACAGCT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AGGGGCACAA GAAAGGGGCA AGAACACCCA AGGTACCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCTCCACA CAGCAATAGC 
GAAGAGTACA ACATTTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG TCCCGTGCCA 
CCAGCAAGGA TGTATTTGCA ACAAGATGAA TTAGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACTCCCCCCG TTCGGGGAGC AGCTTCTTCT CCAGCTGCCG TGTCCTATAG CCATCAGTCC 
ACTGCCACTC TGACTCCCTC CCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCA 
GAGGAGACTG GCCACATGCA GCACCAGCCC GACAGGAGAC GGCAGCCTGT GAGTCCTCCT 
CCACCACCAC GGCCGATCTC CCCTCCACAT ACCTATGGCT ACATTTCAGG ACCCCTGGTC 
TCAGATATGG ATACAGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAGGTAGCC 
AAGATGCAAA CCAGAAGGCT TTTGTTACGT GGGCTTGAGC AGACACCTGC CTCCAGTGTT 
GGGGACCTGG AGAGCTCTGT CACGGGGTCC ATGATCAACG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGT GTTAGTTCTT CGGATGGCTC CTTTTTCACT 
GATGCTGACT TTGCCCAGGC AGTCGCAGCA GCGGCAGAGT ATGCTGGTCT GAAAGTGGCA 
CGACGGCAAA TGCAGGATGC TGCTGGCCGT CGACATTTTC ATGCGTCTCA GTGCCCTAGG 
CCCACAAGTC CTGTGTCTAC AGACAGCAAC ATGAGTGCCG CCGTAATGCA GAAAACCAGA 
CCAGCCAAGA AACCGAAACA CCAGCCAGGA CATCTGCGCA GAGAAACCTA CACAGATGAT 
CTTCCACCAC CTCCTGTGCC GCCACCTGCT ATAAAGTCAC CTACTGCCCA ATCCAAGACA 
CAGCTGGAAG TACGACCTGT AGTGGTGCCA AAACTCCCTT CTGTGGATGC AAGAACAGAC 
AGATCATCAG ACAGAAAAGG AAGCAGTTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAG 
GTTGTTGACA TGCGAACAAA TCCAGGTGAT CCCAGAGAAG CACAGGAACA GCAAAATGAC 
GGGAAAGGAC GTGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTATTGTAGA CCTACTTTTC CAACATCAAA TAATCCCAGA 
GATCCCAGTT CCTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTAC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796993.1
CDS	1..4839
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941504.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941504.2

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGACG GACTGTAACT TTTCAGTGTG AAGCAACCGG AAATCCTCAA 
CCAGCTATTT TTTGGAGGAG AGAAGGGAGT CAGAATCTAC TTTTCTCATA TCAACCACCA 
CAGTCATCCA GCCGATTTTC AGTCTCCCAG ACTGGCGACC TCACAATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA CATCTGCCAG ACTTTAAATG TTGCTGGAAG CATCATCACA 
AAGGCATATT TGGAAGTTAC AGATGTGATT GCAGATCGGC CTCCCCCAGT TATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCTGTGGAT GGCACTTTCG TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACCAT TCTGTGGAGA AAGGATGGAG TCCTCGTTTC AACCCAAGAC 
TCTCGAATCA AACAGTTGGA GAATGGAGTA CTGCAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AAGCAACATG GAGTGCTTAC 
ATTGAAGTTC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCTACTGA CCCAAATTTA 
ATCCCTAGTG CCCCATCAAA ACCTGAAGTG ACAGATGTCA GCAGAAATAC AGTCACATTA 
TCATGGCAAC CAAATTTGAA TTCAGGAGCA ACTCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC AGAAACATTT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCTAATGCA 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACACAAGA TGTCCTACCA 
ACAAGTCAGG GGGTGGACCA CAAGCAGGTC CAGAGAGAGC TGGGGAATGC TGTTCTGCAC 
CTCCACAACC CCACCGTCCT TTCTTCCTCT TCCATCGAAG TGCACTGGAC AGTAGATCAA 
CAGTCTCAGT ATATACAAGG ATATAAAATT CTCTATCGGC CATCTGGAGC CAACCACGGA 
GAATCAGACT GGTTAGTTTT TGAAGTGAGG ACGCCAGCCA AAAACAGTGT GGTTATCCCT 
GATCTCAGAA AGGGAGTCAA CTATGAAATT AAGGCTCGCC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATCAA GTTCGCCAAA ACCCTGGAAG AAGCACCCAG TGCCCCACCC 
CAAGGTGTAA CTGTATCCAA GAATGATGGA AACGGAACTG CAATTCTAGT TAGTTGGCAG 
CCACCTCCAG AAGACACTCA AAATGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTC GATACCACAT CAACAAAACA GTGGATGGTT CCACCTTTTC CGTGGTCATT 
CCCTTTCTTG TTCCTGGAAT CCGATACAGT GTGGAAGTGG CAGCCAGCAC TGGGGCTGGG 
TCTGGGGTAA AGAGTGAGCC TCAGTTCATC CAGCTGGATG CCCATGGAAA CCCTGTGTCA 
CCTGAGGACC AAGTCAGCCT CGCTCAGCAG ATTTCAGATG TGGTGAAGCA GCCGGCCTTC 
ATAGCAGGTA TTGGAGCAGC CTGTTGGATC ATCCTCATGG TCTTCAGCAT CTGGCTTTAT 
CGACACCGCA AGAAGAGAAA CGGACTTACT AGTACTTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAC CAGAGAGGAG GCGAAGCTGT CAGCAGTGGA 
GGGAGGCCCG GACTTCTCAA CATCAGTGAA CCTGCCACGC AGCCATGGCT GGCAGACACG 
TGGCCTAATA CTGGCAACAA CCACAATGAC TGCTCCATCA GCTGCTGCAC GGCAGGCAAT 
GGAAACAGCG ACAGCAACCT CACTACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCTACTCCTT ATGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGAGCAAAT GACACAGTTC CTCAAACTAT CCCATACAAC 
CAATCATACG ACCAGAACAC AGGAGGATCC TACAACAGCT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AGGGGCACAA GAAAGGGGCA AGAACACCCA AGGTACCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCTCCACA CAGCAATAGC 
GAAGAGTACA ACATTTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG TCCCGTGCCA 
CCAGCAAGGA TGTATTTGCA ACAAGATGAA TTAGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACTCCCCCCG TTCGGGGAGC AGCTTCTTCT CCAGCTGCCG TGTCCTATAG CCATCAGTCC 
ACTGCCACTC TGACTCCCTC CCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCA 
GAGGAGACTG GCCACATGCA GCACCAGCCC GACAGGAGAC GGCAGCCTGT GAGTCCTCCT 
CCACCACCAC GGCCGATCTC CCCTCCACAT ACCTATGGCT ACATTTCAGG ACCCCTGGTC 
TCAGATATGG ATACAGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAGGTAGCC 
AAGATGCAAA CCAGAAGGCT TTTGTTACGT GGGCTTGAGC AGACACCTGC CTCCAGTGTT 
GGGGACCTGG AGAGCTCTGT CACGGGGTCC ATGATCAACG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGT GTTAGTTCTT CGGATGGCTC CTTTTTCACT 
GATGCTGACT TTGCCCAGGC AGTCGCAGCA GCGGCAGAGT ATGCTGGTCT GAAAGTGGCA 
CGACGGCAAA TGCAGGATGC TGCTGGCCGT CGACATTTTC ATGCGTCTCA GTGCCCTAGG 
CCCACAAGTC CTGTGTCTAC AGACAGCAAC ATGAGTGCCG CCGTAATGCA GAAAACCAGA 
CCAGCCAAGA AACCGAAACA CCAGCCAGGA CATCTGCGCA GAGAAACCTA CACAGATGAT 
CTTCCACCAC CTCCTGTGCC GCCACCTGCT ATAAAGTCAC CTACTGCCCA ATCCAAGACA 
CAGCTGGAAG TACGACCTGT AGTGGTGCCA AAACTCCCTT CTGTGGATGC AAGAACAGAC 
AGATCATCAG ACAGAAAAGG AAGCAGTTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAG 
GTTGTTGACA TGCGAACAAA TCCAGGTGAT CCCAGAGAAG CACAGGAACA GCAAAATGAC 
GGGAAAGGAC GTGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTATTGTAGA CCTACTTTTC CAACATCAAA TAATCCCAGA 
GATCCCAGTT CCTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTAC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_516592.6 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_516592.4 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X14, mRNA.

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CloneID	OPc105022
Clone ID Related Accession (Same CDS sequence)	XM_516592.6
Accession Version	XM_516592.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_516592.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGGAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC CGGACTTCTC 
AACATCAGTG AACCTGCCAC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCTACTCC TTATGCCACC ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGCGG GGACTCTGGC GAGAAGCACT GGAAACCACT GGGACAGCAG 
AAACAAGAAG TGGCACCAGT TCAGTACAAC ATCATGGAGC AAAACAAGCT GAACAAAGAT 
TATCGAGCAA ATGACACAGT TCCTCAAACT ATCCCATACA ACCAATCATA CGACCAGAAC 
ACAGGAGGAT CCTACAACAG CTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAGGGGCAC 
AAGAAAGGGG CAAGAACACC CAAGGTACCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCTCCA CACAGCAATA GCGAAGAGTA CAACATTTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG 
CAACAAGATG AATTAGAAGA GGAGGAAGAT GAACGAGGCC CCACTCCCCC CGTTCGGGGA 
GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT AGCCATCAGT CCACTGCCAC TCTGACTCCC 
TCCCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CAGAGGAGAC TGGCCACATG 
CAGCACCAGC CCGACAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC ACGGCCGATC 
TCCCCTCCAC ATACCTATGG CTACATTTCA GGACCCCTGG TCTCAGATAT GGATACAGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAGGTAG CCAAGATGCA AACCAGAAGG 
CTTTTGTTAC GTGGGCTTGA GCAGACACCT GCCTCCAGTG TTGGGGACCT GGAGAGCTCT 
GTCACGGGGT CCATGATCAA CGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GTGTTAGTTC TTCGGATGGC TCCTTTTTCA CTGATGCTGA CTTTGCCCAG 
GCAGTCGCAG CAGCGGCAGA GTATGCTGGT CTGAAAGTGG CACGACGGCA AATGCAGGAT 
GCTGCTGGCC GTCGACATTT TCATGCGTCT CAGTGCCCTA GGCCCACAAG TCCTGTGTCT 
ACAGACAGCA ACATGAGTGC CGCCGTAATG CAGAAAACCA GACCAGCCAA GAAACCGAAA 
CACCAGCCAG GACATCTGCG CAGAGAAACC TACACAGATG ATCTTCCACC ACCTCCTGTG 
CCGCCACCTG CTATAAAGTC ACCTACTGCC CAATCCAAGA CACAGCTGGA AGTACGACCT 
GTAGTGGTGC CAAAACTCCC TTCTGTGGAT GCAAGAACAG ACAGATCATC AGACAGAAAA 
GGAAGCAGTT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AGGTTGTTGA CATGCGAACA 
AATCCAGGTG ATCCCAGAGA AGCACAGGAA CAGCAAAATG ACGGGAAAGG ACGTGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTATTGTA GACCTACTTT TCCAACATCA AATAATCCCA GAGATCCCAG TTCCTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT ACTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_516592.4
CDS	1007..5827
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_516592.6
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_516592.6

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGGAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC CGGACTTCTC 
AACATCAGTG AACCTGCCAC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCTACTCC TTATGCCACC ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGCGG GGACTCTGGC GAGAAGCACT GGAAACCACT GGGACAGCAG 
AAACAAGAAG TGGCACCAGT TCAGTACAAC ATCATGGAGC AAAACAAGCT GAACAAAGAT 
TATCGAGCAA ATGACACAGT TCCTCAAACT ATCCCATACA ACCAATCATA CGACCAGAAC 
ACAGGAGGAT CCTACAACAG CTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAGGGGCAC 
AAGAAAGGGG CAAGAACACC CAAGGTACCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCTCCA CACAGCAATA GCGAAGAGTA CAACATTTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG 
CAACAAGATG AATTAGAAGA GGAGGAAGAT GAACGAGGCC CCACTCCCCC CGTTCGGGGA 
GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT AGCCATCAGT CCACTGCCAC TCTGACTCCC 
TCCCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CAGAGGAGAC TGGCCACATG 
CAGCACCAGC CCGACAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC ACGGCCGATC 
TCCCCTCCAC ATACCTATGG CTACATTTCA GGACCCCTGG TCTCAGATAT GGATACAGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAGGTAG CCAAGATGCA AACCAGAAGG 
CTTTTGTTAC GTGGGCTTGA GCAGACACCT GCCTCCAGTG TTGGGGACCT GGAGAGCTCT 
GTCACGGGGT CCATGATCAA CGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GTGTTAGTTC TTCGGATGGC TCCTTTTTCA CTGATGCTGA CTTTGCCCAG 
GCAGTCGCAG CAGCGGCAGA GTATGCTGGT CTGAAAGTGG CACGACGGCA AATGCAGGAT 
GCTGCTGGCC GTCGACATTT TCATGCGTCT CAGTGCCCTA GGCCCACAAG TCCTGTGTCT 
ACAGACAGCA ACATGAGTGC CGCCGTAATG CAGAAAACCA GACCAGCCAA GAAACCGAAA 
CACCAGCCAG GACATCTGCG CAGAGAAACC TACACAGATG ATCTTCCACC ACCTCCTGTG 
CCGCCACCTG CTATAAAGTC ACCTACTGCC CAATCCAAGA CACAGCTGGA AGTACGACCT 
GTAGTGGTGC CAAAACTCCC TTCTGTGGAT GCAAGAACAG ACAGATCATC AGACAGAAAA 
GGAAGCAGTT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AGGTTGTTGA CATGCGAACA 
AATCCAGGTG ATCCCAGAGA AGCACAGGAA CAGCAAAATG ACGGGAAAGG ACGTGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTATTGTA GACCTACTTT TCCAACATCA AATAATCCCA GAGATCCCAG TTCCTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT ACTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_003309895.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003309943.2 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OPc105023
Clone ID Related Accession (Same CDS sequence)	XM_003309895.4
Accession Version	XM_003309895.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_003309895.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGGAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC CGGACTTCTC 
AACATCAGTG AACCTGCCAC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003309943.2
CDS	1007..5662
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_003309895.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003309895.4

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGGAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC CGGACTTCTC 
AACATCAGTG AACCTGCCAC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941508.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796997.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OPc105024
Clone ID Related Accession (Same CDS sequence)	XM_016941508.2
Accession Version	XM_016941508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016941508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGGAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC ATCAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA TGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA GAGGCTTATT ATCGAGCAAA TGACACAGTT 
CCTCAAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCC GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACAGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGATGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTGGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCTGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACCGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTGTGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796997.1
CDS	204..4823
Translation	MLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941508.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941508.2

ORF Insert Sequence:

ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGGAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC ATCAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA TGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA GAGGCTTATT ATCGAGCAAA TGACACAGTT 
CCTCAAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCC GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACAGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGATGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTGGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCTGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACCGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTGTGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941496.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796985.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

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CloneID	OPc82301
Clone ID Related Accession (Same CDS sequence)	XM_016941496.1
Accession Version	XM_016941496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCCGGACT TCTCAACATC AGTGAACCTG CCACGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTATGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCATG 
GAGCAAAACA AGCTGAACAA AGGTGAAGAG GCTTATTATC GAGCAAATGA CACAGTTCCT 
CAAACTATCC CATACAACCA ATCATACGAC CAGAACACAG GAGGATCCTA CAACAGCTCA 
GACCGGGGCA GTAGTACATC TGGGAGTCAG GGGCACAAGA AAGGGGCAAG AACACCCAAG 
GTACCAAAAC AGGGTGGCAT GAACTGGGCA GACCTGCTTC CTCCTCCCCC AGCACATCCT 
CCTCCACACA GCAATAGCGA AGAGTACAAC ATTTCTGTAG ATGAAAGCTA TGACCAAGAA 
ATGCCATGTC CCGTGCCACC AGCAAGGATG TATTTGCAAC AAGATGAATT AGAAGAGGAG 
GAAGATGAAC GAGGCCCCAC TCCCCCCGTT CGGGGAGCAG CTTCTTCTCC AGCTGCCGTG 
TCCTATAGCC ATCAGTCCAC TGCCACTCTG ACTCCCTCCC CACAGGAAGA ACTCCAGCCC 
ATGTTACAGG ATTGTCCAGA GGAGACTGGC CACATGCAGC ACCAGCCCGA CAGGAGACGG 
CAGCCTGTGA GTCCTCCTCC ACCACCACGG CCGATCTCCC CTCCACATAC CTATGGCTAC 
ATTTCAGGAC CCCTGGTCTC AGATATGGAT ACAGATGCGC CAGAAGAGGA AGAAGACGAA 
GCCGACATGG AGGTAGCCAA GATGCAAACC AGAAGGCTTT TGTTACGTGG GCTTGAGCAG 
ACACCTGCCT CCAGTGTTGG GGACCTGGAG AGCTCTGTCA CGGGGTCCAT GATCAACGGC 
TGGGGCTCAG CCTCAGAGGA GGACAACATT TCCAGCGGAC GCTCCAGTGT TAGTTCTTCG 
GATGGCTCCT TTTTCACTGA TGCTGACTTT GCCCAGGCAG TCGCAGCAGC GGCAGAGTAT 
GCTGGTCTGA AAGTGGCACG ACGGCAAATG CAGGATGCTG CTGGCCGTCG ACATTTTCAT 
GCGTCTCAGT GCCCTAGGCC CACAAGTCCT GTGTCTACAG ACAGCAACAT GAGTGCCGCC 
GTAATGCAGA AAACCAGACC AGCCAAGAAA CCGAAACACC AGCCAGGACA TCTGCGCAGA 
GAAACCTACA CAGATGATCT TCCACCACCT CCTGTGCCGC CACCTGCTAT AAAGTCACCT 
ACTGCCCAAT CCAAGACACA GCTGGAAGTA CGACCTGTAG TGGTGCCAAA ACTCCCTTCT 
GTGGATGCAA GAACAGACAG ATCATCAGAC AGAAAAGGAA GCAGTTACAA GGGGAGAGAA 
GTATTGGATG GAAGACAGGT TGTTGACATG CGAACAAATC CAGGTGATCC CAGAGAAGCA 
CAGGAACAGC AAAATGACGG GAAAGGACGT GGAAACAAGG CAGCAAAACG AGACCTTCCA 
CCAGCAAAGA CTCATCTCAT CCAAGAGGAT ATTCTACCTT ATTGTAGACC TACTTTTCCA 
ACATCAAATA ATCCCAGAGA TCCCAGTTCC TCAAGCTCAA TGTCATCAAG AGGATCAGGA 
AGCAGACAAA GAGAACAAGC AAATGTAGGT CGAAGAAATA TTGCAGAAAT GCAGGTACTT 
GGAGGATATG AAAGAGGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796985.1
CDS	293..5269
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941496.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941496.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCCGGACT TCTCAACATC AGTGAACCTG CCACGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTATGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCATG 
GAGCAAAACA AGCTGAACAA AGGTGAAGAG GCTTATTATC GAGCAAATGA CACAGTTCCT 
CAAACTATCC CATACAACCA ATCATACGAC CAGAACACAG GAGGATCCTA CAACAGCTCA 
GACCGGGGCA GTAGTACATC TGGGAGTCAG GGGCACAAGA AAGGGGCAAG AACACCCAAG 
GTACCAAAAC AGGGTGGCAT GAACTGGGCA GACCTGCTTC CTCCTCCCCC AGCACATCCT 
CCTCCACACA GCAATAGCGA AGAGTACAAC ATTTCTGTAG ATGAAAGCTA TGACCAAGAA 
ATGCCATGTC CCGTGCCACC AGCAAGGATG TATTTGCAAC AAGATGAATT AGAAGAGGAG 
GAAGATGAAC GAGGCCCCAC TCCCCCCGTT CGGGGAGCAG CTTCTTCTCC AGCTGCCGTG 
TCCTATAGCC ATCAGTCCAC TGCCACTCTG ACTCCCTCCC CACAGGAAGA ACTCCAGCCC 
ATGTTACAGG ATTGTCCAGA GGAGACTGGC CACATGCAGC ACCAGCCCGA CAGGAGACGG 
CAGCCTGTGA GTCCTCCTCC ACCACCACGG CCGATCTCCC CTCCACATAC CTATGGCTAC 
ATTTCAGGAC CCCTGGTCTC AGATATGGAT ACAGATGCGC CAGAAGAGGA AGAAGACGAA 
GCCGACATGG AGGTAGCCAA GATGCAAACC AGAAGGCTTT TGTTACGTGG GCTTGAGCAG 
ACACCTGCCT CCAGTGTTGG GGACCTGGAG AGCTCTGTCA CGGGGTCCAT GATCAACGGC 
TGGGGCTCAG CCTCAGAGGA GGACAACATT TCCAGCGGAC GCTCCAGTGT TAGTTCTTCG 
GATGGCTCCT TTTTCACTGA TGCTGACTTT GCCCAGGCAG TCGCAGCAGC GGCAGAGTAT 
GCTGGTCTGA AAGTGGCACG ACGGCAAATG CAGGATGCTG CTGGCCGTCG ACATTTTCAT 
GCGTCTCAGT GCCCTAGGCC CACAAGTCCT GTGTCTACAG ACAGCAACAT GAGTGCCGCC 
GTAATGCAGA AAACCAGACC AGCCAAGAAA CCGAAACACC AGCCAGGACA TCTGCGCAGA 
GAAACCTACA CAGATGATCT TCCACCACCT CCTGTGCCGC CACCTGCTAT AAAGTCACCT 
ACTGCCCAAT CCAAGACACA GCTGGAAGTA CGACCTGTAG TGGTGCCAAA ACTCCCTTCT 
GTGGATGCAA GAACAGACAG ATCATCAGAC AGAAAAGGAA GCAGTTACAA GGGGAGAGAA 
GTATTGGATG GAAGACAGGT TGTTGACATG CGAACAAATC CAGGTGATCC CAGAGAAGCA 
CAGGAACAGC AAAATGACGG GAAAGGACGT GGAAACAAGG CAGCAAAACG AGACCTTCCA 
CCAGCAAAGA CTCATCTCAT CCAAGAGGAT ATTCTACCTT ATTGTAGACC TACTTTTCCA 
ACATCAAATA ATCCCAGAGA TCCCAGTTCC TCAAGCTCAA TGTCATCAAG AGGATCAGGA 
AGCAGACAAA GAGAACAAGC AAATGTAGGT CGAAGAAATA TTGCAGAAAT GCAGGTACTT 
GGAGGATATG AAAGAGGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941497.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796986.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

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CloneID	OPc82302
Clone ID Related Accession (Same CDS sequence)	XM_016941497.1
Accession Version	XM_016941497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTTT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC TGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCA 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATTTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT TATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT CGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCCGGAC TTCTCAACAT CAGTGAACCT GCCACGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGGTGAAGA GGCTTATTAT CGAGCAAATG ACACAGTTCC TCAAACTATC 
CCATACAACC AATCATACGA CCAGAACACA GGAGGATCCT ACAACAGCTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA GGGGCACAAG AAAGGGGCAA GAACACCCAA GGTACCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCTCCACAC 
AGCAATAGCG AAGAGTACAA CATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT 
CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAAGATGAAT TAGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CTCCCCCCGT TCGGGGAGCA GCTTCTTCTC CAGCTGCCGT GTCCTATAGC 
CATCAGTCCA CTGCCACTCT GACTCCCTCC CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCAG AGGAGACTGG CCACATGCAG CACCAGCCCG ACAGGAGACG GCAGCCTGTG 
AGTCCTCCTC CACCACCACG GCCGATCTCC CCTCCACATA CCTATGGCTA CATTTCAGGA 
CCCCTGGTCT CAGATATGGA TACAGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAGGTAGCCA AGATGCAAAC CAGAAGGCTT TTGTTACGTG GGCTTGAGCA GACACCTGCC 
TCCAGTGTTG GGGACCTGGA GAGCTCTGTC ACGGGGTCCA TGATCAACGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGTG TTAGTTCTTC GGATGGCTCC 
TTTTTCACTG ATGCTGACTT TGCCCAGGCA GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG 
AAAGTGGCAC GACGGCAAAT GCAGGATGCT GCTGGCCGTC GACATTTTCA TGCGTCTCAG 
TGCCCTAGGC CCACAAGTCC TGTGTCTACA GACAGCAACA TGAGTGCCGC CGTAATGCAG 
AAAACCAGAC CAGCCAAGAA ACCGAAACAC CAGCCAGGAC ATCTGCGCAG AGAAACCTAC 
ACAGATGATC TTCCACCACC TCCTGTGCCG CCACCTGCTA TAAAGTCACC TACTGCCCAA 
TCCAAGACAC AGCTGGAAGT ACGACCTGTA GTGGTGCCAA AACTCCCTTC TGTGGATGCA 
AGAACAGACA GATCATCAGA CAGAAAAGGA AGCAGTTACA AGGGGAGAGA AGTATTGGAT 
GGAAGACAGG TTGTTGACAT GCGAACAAAT CCAGGTGATC CCAGAGAAGC ACAGGAACAG 
CAAAATGACG GGAAAGGACG TGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TATTGTAGAC CTACTTTTCC AACATCAAAT 
AATCCCAGAG ATCCCAGTTC CTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTACT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796986.1
CDS	293..5260
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941497.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941497.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTTT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC TGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCA 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATTTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT TATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT CGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCCGGAC TTCTCAACAT CAGTGAACCT GCCACGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGGTGAAGA GGCTTATTAT CGAGCAAATG ACACAGTTCC TCAAACTATC 
CCATACAACC AATCATACGA CCAGAACACA GGAGGATCCT ACAACAGCTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA GGGGCACAAG AAAGGGGCAA GAACACCCAA GGTACCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCTCCACAC 
AGCAATAGCG AAGAGTACAA CATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT 
CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAAGATGAAT TAGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CTCCCCCCGT TCGGGGAGCA GCTTCTTCTC CAGCTGCCGT GTCCTATAGC 
CATCAGTCCA CTGCCACTCT GACTCCCTCC CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCAG AGGAGACTGG CCACATGCAG CACCAGCCCG ACAGGAGACG GCAGCCTGTG 
AGTCCTCCTC CACCACCACG GCCGATCTCC CCTCCACATA CCTATGGCTA CATTTCAGGA 
CCCCTGGTCT CAGATATGGA TACAGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAGGTAGCCA AGATGCAAAC CAGAAGGCTT TTGTTACGTG GGCTTGAGCA GACACCTGCC 
TCCAGTGTTG GGGACCTGGA GAGCTCTGTC ACGGGGTCCA TGATCAACGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGTG TTAGTTCTTC GGATGGCTCC 
TTTTTCACTG ATGCTGACTT TGCCCAGGCA GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG 
AAAGTGGCAC GACGGCAAAT GCAGGATGCT GCTGGCCGTC GACATTTTCA TGCGTCTCAG 
TGCCCTAGGC CCACAAGTCC TGTGTCTACA GACAGCAACA TGAGTGCCGC CGTAATGCAG 
AAAACCAGAC CAGCCAAGAA ACCGAAACAC CAGCCAGGAC ATCTGCGCAG AGAAACCTAC 
ACAGATGATC TTCCACCACC TCCTGTGCCG CCACCTGCTA TAAAGTCACC TACTGCCCAA 
TCCAAGACAC AGCTGGAAGT ACGACCTGTA GTGGTGCCAA AACTCCCTTC TGTGGATGCA 
AGAACAGACA GATCATCAGA CAGAAAAGGA AGCAGTTACA AGGGGAGAGA AGTATTGGAT 
GGAAGACAGG TTGTTGACAT GCGAACAAAT CCAGGTGATC CCAGAGAAGC ACAGGAACAG 
CAAAATGACG GGAAAGGACG TGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TATTGTAGAC CTACTTTTCC AACATCAAAT 
AATCCCAGAG ATCCCAGTTC CTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTACT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941498.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796987.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

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CloneID	OPc82303
Clone ID Related Accession (Same CDS sequence)	XM_016941498.1
Accession Version	XM_016941498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCCGGACT TCTCAACATC AGTGAACCTG CCACGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTATGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCATG 
GAGCAAAACA AGCTGAACAA AGATTATCGA GCAAATGACA CAGTTCCTCA AACTATCCCA 
TACAACCAAT CATACGACCA GAACACAGGA GGATCCTACA ACAGCTCAGA CCGGGGCAGT 
AGTACATCTG GGAGTCAGGG GCACAAGAAA GGGGCAAGAA CACCCAAGGT ACCAAAACAG 
GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CACATCCTCC TCCACACAGC 
AATAGCGAAG AGTACAACAT TTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGTCCC 
GTGCCACCAG CAAGGATGTA TTTGCAACAA GATGAATTAG AAGAGGAGGA AGATGAACGA 
GGCCCCACTC CCCCCGTTCG GGGAGCAGCT TCTTCTCCAG CTGCCGTGTC CTATAGCCAT 
CAGTCCACTG CCACTCTGAC TCCCTCCCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCAGAGG AGACTGGCCA CATGCAGCAC CAGCCCGACA GGAGACGGCA GCCTGTGAGT 
CCTCCTCCAC CACCACGGCC GATCTCCCCT CCACATACCT ATGGCTACAT TTCAGGACCC 
CTGGTCTCAG ATATGGATAC AGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAG 
GTAGCCAAGA TGCAAACCAG AAGGCTTTTG TTACGTGGGC TTGAGCAGAC ACCTGCCTCC 
AGTGTTGGGG ACCTGGAGAG CTCTGTCACG GGGTCCATGA TCAACGGCTG GGGCTCAGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGTGTTA GTTCTTCGGA TGGCTCCTTT 
TTCACTGATG CTGACTTTGC CCAGGCAGTC GCAGCAGCGG CAGAGTATGC TGGTCTGAAA 
GTGGCACGAC GGCAAATGCA GGATGCTGCT GGCCGTCGAC ATTTTCATGC GTCTCAGTGC 
CCTAGGCCCA CAAGTCCTGT GTCTACAGAC AGCAACATGA GTGCCGCCGT AATGCAGAAA 
ACCAGACCAG CCAAGAAACC GAAACACCAG CCAGGACATC TGCGCAGAGA AACCTACACA 
GATGATCTTC CACCACCTCC TGTGCCGCCA CCTGCTATAA AGTCACCTAC TGCCCAATCC 
AAGACACAGC TGGAAGTACG ACCTGTAGTG GTGCCAAAAC TCCCTTCTGT GGATGCAAGA 
ACAGACAGAT CATCAGACAG AAAAGGAAGC AGTTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAGGTTG TTGACATGCG AACAAATCCA GGTGATCCCA GAGAAGCACA GGAACAGCAA 
AATGACGGGA AAGGACGTGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGACT 
CATCTCATCC AAGAGGATAT TCTACCTTAT TGTAGACCTA CTTTTCCAAC ATCAAATAAT 
CCCAGAGATC CCAGTTCCTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAAATGC AGGTACTTGG AGGATATGAA 
AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796987.1
CDS	293..5257
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941498.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941498.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCCGGACT TCTCAACATC AGTGAACCTG CCACGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTATGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCATG 
GAGCAAAACA AGCTGAACAA AGATTATCGA GCAAATGACA CAGTTCCTCA AACTATCCCA 
TACAACCAAT CATACGACCA GAACACAGGA GGATCCTACA ACAGCTCAGA CCGGGGCAGT 
AGTACATCTG GGAGTCAGGG GCACAAGAAA GGGGCAAGAA CACCCAAGGT ACCAAAACAG 
GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CACATCCTCC TCCACACAGC 
AATAGCGAAG AGTACAACAT TTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGTCCC 
GTGCCACCAG CAAGGATGTA TTTGCAACAA GATGAATTAG AAGAGGAGGA AGATGAACGA 
GGCCCCACTC CCCCCGTTCG GGGAGCAGCT TCTTCTCCAG CTGCCGTGTC CTATAGCCAT 
CAGTCCACTG CCACTCTGAC TCCCTCCCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCAGAGG AGACTGGCCA CATGCAGCAC CAGCCCGACA GGAGACGGCA GCCTGTGAGT 
CCTCCTCCAC CACCACGGCC GATCTCCCCT CCACATACCT ATGGCTACAT TTCAGGACCC 
CTGGTCTCAG ATATGGATAC AGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAG 
GTAGCCAAGA TGCAAACCAG AAGGCTTTTG TTACGTGGGC TTGAGCAGAC ACCTGCCTCC 
AGTGTTGGGG ACCTGGAGAG CTCTGTCACG GGGTCCATGA TCAACGGCTG GGGCTCAGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGTGTTA GTTCTTCGGA TGGCTCCTTT 
TTCACTGATG CTGACTTTGC CCAGGCAGTC GCAGCAGCGG CAGAGTATGC TGGTCTGAAA 
GTGGCACGAC GGCAAATGCA GGATGCTGCT GGCCGTCGAC ATTTTCATGC GTCTCAGTGC 
CCTAGGCCCA CAAGTCCTGT GTCTACAGAC AGCAACATGA GTGCCGCCGT AATGCAGAAA 
ACCAGACCAG CCAAGAAACC GAAACACCAG CCAGGACATC TGCGCAGAGA AACCTACACA 
GATGATCTTC CACCACCTCC TGTGCCGCCA CCTGCTATAA AGTCACCTAC TGCCCAATCC 
AAGACACAGC TGGAAGTACG ACCTGTAGTG GTGCCAAAAC TCCCTTCTGT GGATGCAAGA 
ACAGACAGAT CATCAGACAG AAAAGGAAGC AGTTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAGGTTG TTGACATGCG AACAAATCCA GGTGATCCCA GAGAAGCACA GGAACAGCAA 
AATGACGGGA AAGGACGTGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGACT 
CATCTCATCC AAGAGGATAT TCTACCTTAT TGTAGACCTA CTTTTCCAAC ATCAAATAAT 
CCCAGAGATC CCAGTTCCTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAAATGC AGGTACTTGG AGGATATGAA 
AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941499.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796988.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc82304
Clone ID Related Accession (Same CDS sequence)	XM_016941499.1
Accession Version	XM_016941499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTTT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC TGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCA 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATTTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT TATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT CGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCCGGAC TTCTCAACAT CAGTGAACCT GCCACGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796988.1
CDS	209..5164
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941499.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941499.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTTT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC TGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCA 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATTTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT TATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT CGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCCGGAC TTCTCAACAT CAGTGAACCT GCCACGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941500.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796989.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPc82305
Clone ID Related Accession (Same CDS sequence)	XM_016941500.1
Accession Version	XM_016941500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CTACTCCTTA TGCCACCACT CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGCGGGGA CTCTGGCGAG AAGCACTGGA AACCACTGGG ACAGCAGAAA 
CAAGAAGTGG CACCAGTTCA GTACAACATC ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA 
GAGGCTTATT ATCGAGCAAA TGACACAGTT CCTCAAACTA TCCCATACAA CCAATCATAC 
GACCAGAACA CAGGAGGATC CTACAACAGC TCAGACCGGG GCAGTAGTAC ATCTGGGAGT 
CAGGGGCACA AGAAAGGGGC AAGAACACCC AAGGTACCAA AACAGGGTGG CATGAACTGG 
GCAGACCTGC TTCCTCCTCC CCCAGCACAT CCTCCTCCAC ACAGCAATAG CGAAGAGTAC 
AACATTTCTG TAGATGAAAG CTATGACCAA GAAATGCCAT GTCCCGTGCC ACCAGCAAGG 
ATGTATTTGC AACAAGATGA ATTAGAAGAG GAGGAAGATG AACGAGGCCC CACTCCCCCC 
GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC GTGTCCTATA GCCATCAGTC CACTGCCACT 
CTGACTCCCT CCCCACAGGA AGAACTCCAG CCCATGTTAC AGGATTGTCC AGAGGAGACT 
GGCCACATGC AGCACCAGCC CGACAGGAGA CGGCAGCCTG TGAGTCCTCC TCCACCACCA 
CGGCCGATCT CCCCTCCACA TACCTATGGC TACATTTCAG GACCCCTGGT CTCAGATATG 
GATACAGATG CGCCAGAAGA GGAAGAAGAC GAAGCCGACA TGGAGGTAGC CAAGATGCAA 
ACCAGAAGGC TTTTGTTACG TGGGCTTGAG CAGACACCTG CCTCCAGTGT TGGGGACCTG 
GAGAGCTCTG TCACGGGGTC CATGATCAAC GGCTGGGGCT CAGCCTCAGA GGAGGACAAC 
ATTTCCAGCG GACGCTCCAG TGTTAGTTCT TCGGATGGCT CCTTTTTCAC TGATGCTGAC 
TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG TATGCTGGTC TGAAAGTGGC ACGACGGCAA 
ATGCAGGATG CTGCTGGCCG TCGACATTTT CATGCGTCTC AGTGCCCTAG GCCCACAAGT 
CCTGTGTCTA CAGACAGCAA CATGAGTGCC GCCGTAATGC AGAAAACCAG ACCAGCCAAG 
AAACCGAAAC ACCAGCCAGG ACATCTGCGC AGAGAAACCT ACACAGATGA TCTTCCACCA 
CCTCCTGTGC CGCCACCTGC TATAAAGTCA CCTACTGCCC AATCCAAGAC ACAGCTGGAA 
GTACGACCTG TAGTGGTGCC AAAACTCCCT TCTGTGGATG CAAGAACAGA CAGATCATCA 
GACAGAAAAG GAAGCAGTTA CAAGGGGAGA GAAGTATTGG ATGGAAGACA GGTTGTTGAC 
ATGCGAACAA ATCCAGGTGA TCCCAGAGAA GCACAGGAAC AGCAAAATGA CGGGAAAGGA 
CGTGGAAACA AGGCAGCAAA ACGAGACCTT CCACCAGCAA AGACTCATCT CATCCAAGAG 
GATATTCTAC CTTATTGTAG ACCTACTTTT CCAACATCAA ATAATCCCAG AGATCCCAGT 
TCCTCAAGCT CAATGTCATC AAGAGGATCA GGAAGCAGAC AAAGAGAACA AGCAAATGTA 
GGTCGAAGAA ATATTGCAGA AATGCAGGTA CTTGGAGGAT ATGAAAGAGG AGAAGATAAT 
AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796989.1
CDS	293..5242
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941500.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941500.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CTACTCCTTA TGCCACCACT CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGCGGGGA CTCTGGCGAG AAGCACTGGA AACCACTGGG ACAGCAGAAA 
CAAGAAGTGG CACCAGTTCA GTACAACATC ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA 
GAGGCTTATT ATCGAGCAAA TGACACAGTT CCTCAAACTA TCCCATACAA CCAATCATAC 
GACCAGAACA CAGGAGGATC CTACAACAGC TCAGACCGGG GCAGTAGTAC ATCTGGGAGT 
CAGGGGCACA AGAAAGGGGC AAGAACACCC AAGGTACCAA AACAGGGTGG CATGAACTGG 
GCAGACCTGC TTCCTCCTCC CCCAGCACAT CCTCCTCCAC ACAGCAATAG CGAAGAGTAC 
AACATTTCTG TAGATGAAAG CTATGACCAA GAAATGCCAT GTCCCGTGCC ACCAGCAAGG 
ATGTATTTGC AACAAGATGA ATTAGAAGAG GAGGAAGATG AACGAGGCCC CACTCCCCCC 
GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC GTGTCCTATA GCCATCAGTC CACTGCCACT 
CTGACTCCCT CCCCACAGGA AGAACTCCAG CCCATGTTAC AGGATTGTCC AGAGGAGACT 
GGCCACATGC AGCACCAGCC CGACAGGAGA CGGCAGCCTG TGAGTCCTCC TCCACCACCA 
CGGCCGATCT CCCCTCCACA TACCTATGGC TACATTTCAG GACCCCTGGT CTCAGATATG 
GATACAGATG CGCCAGAAGA GGAAGAAGAC GAAGCCGACA TGGAGGTAGC CAAGATGCAA 
ACCAGAAGGC TTTTGTTACG TGGGCTTGAG CAGACACCTG CCTCCAGTGT TGGGGACCTG 
GAGAGCTCTG TCACGGGGTC CATGATCAAC GGCTGGGGCT CAGCCTCAGA GGAGGACAAC 
ATTTCCAGCG GACGCTCCAG TGTTAGTTCT TCGGATGGCT CCTTTTTCAC TGATGCTGAC 
TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG TATGCTGGTC TGAAAGTGGC ACGACGGCAA 
ATGCAGGATG CTGCTGGCCG TCGACATTTT CATGCGTCTC AGTGCCCTAG GCCCACAAGT 
CCTGTGTCTA CAGACAGCAA CATGAGTGCC GCCGTAATGC AGAAAACCAG ACCAGCCAAG 
AAACCGAAAC ACCAGCCAGG ACATCTGCGC AGAGAAACCT ACACAGATGA TCTTCCACCA 
CCTCCTGTGC CGCCACCTGC TATAAAGTCA CCTACTGCCC AATCCAAGAC ACAGCTGGAA 
GTACGACCTG TAGTGGTGCC AAAACTCCCT TCTGTGGATG CAAGAACAGA CAGATCATCA 
GACAGAAAAG GAAGCAGTTA CAAGGGGAGA GAAGTATTGG ATGGAAGACA GGTTGTTGAC 
ATGCGAACAA ATCCAGGTGA TCCCAGAGAA GCACAGGAAC AGCAAAATGA CGGGAAAGGA 
CGTGGAAACA AGGCAGCAAA ACGAGACCTT CCACCAGCAA AGACTCATCT CATCCAAGAG 
GATATTCTAC CTTATTGTAG ACCTACTTTT CCAACATCAA ATAATCCCAG AGATCCCAGT 
TCCTCAAGCT CAATGTCATC AAGAGGATCA GGAAGCAGAC AAAGAGAACA AGCAAATGTA 
GGTCGAAGAA ATATTGCAGA AATGCAGGTA CTTGGAGGAT ATGAAAGAGG AGAAGATAAT 
AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941501.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796990.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

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CloneID	OPc82306
Clone ID Related Accession (Same CDS sequence)	XM_016941501.1
Accession Version	XM_016941501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CTACTCCTTA TGCCACCACT CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGCGGGGA CTCTGGCGAG AAGCACTGGA AACCACTGGG ACAGCAGAAA 
CAAGAAGTGG CACCAGTTCA GTACAACATC ATGGAGCAAA ACAAGCTGAA CAAAGATTAT 
CGAGCAAATG ACACAGTTCC TCAAACTATC CCATACAACC AATCATACGA CCAGAACACA 
GGAGGATCCT ACAACAGCTC AGACCGGGGC AGTAGTACAT CTGGGAGTCA GGGGCACAAG 
AAAGGGGCAA GAACACCCAA GGTACCAAAA CAGGGTGGCA TGAACTGGGC AGACCTGCTT 
CCTCCTCCCC CAGCACATCC TCCTCCACAC AGCAATAGCG AAGAGTACAA CATTTCTGTA 
GATGAAAGCT ATGACCAAGA AATGCCATGT CCCGTGCCAC CAGCAAGGAT GTATTTGCAA 
CAAGATGAAT TAGAAGAGGA GGAAGATGAA CGAGGCCCCA CTCCCCCCGT TCGGGGAGCA 
GCTTCTTCTC CAGCTGCCGT GTCCTATAGC CATCAGTCCA CTGCCACTCT GACTCCCTCC 
CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCAG AGGAGACTGG CCACATGCAG 
CACCAGCCCG ACAGGAGACG GCAGCCTGTG AGTCCTCCTC CACCACCACG GCCGATCTCC 
CCTCCACATA CCTATGGCTA CATTTCAGGA CCCCTGGTCT CAGATATGGA TACAGATGCG 
CCAGAAGAGG AAGAAGACGA AGCCGACATG GAGGTAGCCA AGATGCAAAC CAGAAGGCTT 
TTGTTACGTG GGCTTGAGCA GACACCTGCC TCCAGTGTTG GGGACCTGGA GAGCTCTGTC 
ACGGGGTCCA TGATCAACGG CTGGGGCTCA GCCTCAGAGG AGGACAACAT TTCCAGCGGA 
CGCTCCAGTG TTAGTTCTTC GGATGGCTCC TTTTTCACTG ATGCTGACTT TGCCCAGGCA 
GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG AAAGTGGCAC GACGGCAAAT GCAGGATGCT 
GCTGGCCGTC GACATTTTCA TGCGTCTCAG TGCCCTAGGC CCACAAGTCC TGTGTCTACA 
GACAGCAACA TGAGTGCCGC CGTAATGCAG AAAACCAGAC CAGCCAAGAA ACCGAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAACCTAC ACAGATGATC TTCCACCACC TCCTGTGCCG 
CCACCTGCTA TAAAGTCACC TACTGCCCAA TCCAAGACAC AGCTGGAAGT ACGACCTGTA 
GTGGTGCCAA AACTCCCTTC TGTGGATGCA AGAACAGACA GATCATCAGA CAGAAAAGGA 
AGCAGTTACA AGGGGAGAGA AGTATTGGAT GGAAGACAGG TTGTTGACAT GCGAACAAAT 
CCAGGTGATC CCAGAGAAGC ACAGGAACAG CAAAATGACG GGAAAGGACG TGGAAACAAG 
GCAGCAAAAC GAGACCTTCC ACCAGCAAAG ACTCATCTCA TCCAAGAGGA TATTCTACCT 
TATTGTAGAC CTACTTTTCC AACATCAAAT AATCCCAGAG ATCCCAGTTC CTCAAGCTCA 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAAA TGCAGGTACT TGGAGGATAT GAAAGAGGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796990.1
CDS	293..5230
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941501.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941501.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CTACTCCTTA TGCCACCACT CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGCGGGGA CTCTGGCGAG AAGCACTGGA AACCACTGGG ACAGCAGAAA 
CAAGAAGTGG CACCAGTTCA GTACAACATC ATGGAGCAAA ACAAGCTGAA CAAAGATTAT 
CGAGCAAATG ACACAGTTCC TCAAACTATC CCATACAACC AATCATACGA CCAGAACACA 
GGAGGATCCT ACAACAGCTC AGACCGGGGC AGTAGTACAT CTGGGAGTCA GGGGCACAAG 
AAAGGGGCAA GAACACCCAA GGTACCAAAA CAGGGTGGCA TGAACTGGGC AGACCTGCTT 
CCTCCTCCCC CAGCACATCC TCCTCCACAC AGCAATAGCG AAGAGTACAA CATTTCTGTA 
GATGAAAGCT ATGACCAAGA AATGCCATGT CCCGTGCCAC CAGCAAGGAT GTATTTGCAA 
CAAGATGAAT TAGAAGAGGA GGAAGATGAA CGAGGCCCCA CTCCCCCCGT TCGGGGAGCA 
GCTTCTTCTC CAGCTGCCGT GTCCTATAGC CATCAGTCCA CTGCCACTCT GACTCCCTCC 
CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCAG AGGAGACTGG CCACATGCAG 
CACCAGCCCG ACAGGAGACG GCAGCCTGTG AGTCCTCCTC CACCACCACG GCCGATCTCC 
CCTCCACATA CCTATGGCTA CATTTCAGGA CCCCTGGTCT CAGATATGGA TACAGATGCG 
CCAGAAGAGG AAGAAGACGA AGCCGACATG GAGGTAGCCA AGATGCAAAC CAGAAGGCTT 
TTGTTACGTG GGCTTGAGCA GACACCTGCC TCCAGTGTTG GGGACCTGGA GAGCTCTGTC 
ACGGGGTCCA TGATCAACGG CTGGGGCTCA GCCTCAGAGG AGGACAACAT TTCCAGCGGA 
CGCTCCAGTG TTAGTTCTTC GGATGGCTCC TTTTTCACTG ATGCTGACTT TGCCCAGGCA 
GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG AAAGTGGCAC GACGGCAAAT GCAGGATGCT 
GCTGGCCGTC GACATTTTCA TGCGTCTCAG TGCCCTAGGC CCACAAGTCC TGTGTCTACA 
GACAGCAACA TGAGTGCCGC CGTAATGCAG AAAACCAGAC CAGCCAAGAA ACCGAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAACCTAC ACAGATGATC TTCCACCACC TCCTGTGCCG 
CCACCTGCTA TAAAGTCACC TACTGCCCAA TCCAAGACAC AGCTGGAAGT ACGACCTGTA 
GTGGTGCCAA AACTCCCTTC TGTGGATGCA AGAACAGACA GATCATCAGA CAGAAAAGGA 
AGCAGTTACA AGGGGAGAGA AGTATTGGAT GGAAGACAGG TTGTTGACAT GCGAACAAAT 
CCAGGTGATC CCAGAGAAGC ACAGGAACAG CAAAATGACG GGAAAGGACG TGGAAACAAG 
GCAGCAAAAC GAGACCTTCC ACCAGCAAAG ACTCATCTCA TCCAAGAGGA TATTCTACCT 
TATTGTAGAC CTACTTTTCC AACATCAAAT AATCCCAGAG ATCCCAGTTC CTCAAGCTCA 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAAA TGCAGGTACT TGGAGGATAT GAAAGAGGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941502.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796991.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

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CloneID	OPc82307
Clone ID Related Accession (Same CDS sequence)	XM_016941502.1
Accession Version	XM_016941502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGC TGTTACTGAT GCTGCTGCTC TCGATGGCTG AGATAACTTC TCCTGTGGGT 
GTCTTGGACA GAACGAAAGC TGGGTCAAGG TGGAAAGGTG AAAAGAATCG CTCCCGTCTT 
CGTCAGGAAG ATTTTCCACC TCGCATTGTT GAACACCCTT CAGACCTGAT TGTCTCAAAA 
GGAGAACCTG CAACTTTGAA CTGCAAAGCT GAAGGCCGCC CCACACCCAC TATTGAATGG 
TACAAAGGGG GAGAGAGAGT GGAGACAGAC AAAGATGACC CTCGCTCACA CCGAATGTTG 
CTGCCGAGTG GATCTTTATT TTTCTTACGT ATAGTACATG GACGGAAAAG TAGACCTGAT 
GAAGGAGTCT ATGTCTGTGT AGCAAGGAAT TACCTTGGAG AGGCTGTGAG CCACAATGCA 
TCGCTGGAAG TAGCCATACT TCGGGATGAC TTCAGACAAA ACCCTTCGGA TGTCATGGTT 
GCAGTAGGAG AGCCTGCAGT AATGGAATGC CAACCTCCAC GAGGCCATCC TGAGCCCACC 
ATTTCATGGA AGAAAGATGG CTCTCCACTG GATGATAAAG ATGAAAGAAT AACTATACGA 
GGAGGAAAGC TCATGATCAC TTACACCCGT AAAAGTGACG CTGGCAAATA TGTTTGTGTT 
GGTACCAATA TGGTTGGGGA ACGTGAGAGT GAAGTAGCCG AGCTGACTGT CTTAGAGAGA 
CCATCATTTG TGAAGAGACC CAGTAACTTG GCAGTAACTG TGGATGACAG TGCAGAATTT 
AAATGTGAGG CCCGAGGTGA CCCTGTACCT ACAGTACGAT GGAGGAAAGA TGATGGAGAG 
CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACCT TGAAAATTAG GAAGGTGACA 
GCTGGTGACA TGGGTTCATA CACTTGTGTT GCAGAAAATA TGGTGGGCAA AGCTGAAGCA 
TCTGCTACTC TGACTGTGCA AGTTGAGTCT GAACCTCCAC ATTTTGTTGT GAAACCCCGT 
GACCAGGTTG TTGCTTTGGG ACGGACTGTA ACTTTTCAGT GTGAAGCAAC CGGAAATCCT 
CAACCAGCTA TTTTTTGGAG GAGAGAAGGG AGTCAGAATC TACTTTTCTC ATATCAACCA 
CCACAGTCAT CCAGCCGATT TTCAGTCTCC CAGACTGGCG ACCTCACAAT TACTAATGTC 
CAGCGATCTG ATGTTGGTTA TTACATCTGC CAGACTTTAA ATGTTGCTGG AAGCATCATC 
ACAAAGGCAT ATTTGGAAGT TACAGATGTG ATTGCAGATC GGCCTCCCCC AGTTATTCGA 
CAAGGTCCTG TGAATCAGAC TGTAGCTGTG GATGGCACTT TCGTCCTCAG CTGTGTGGCC 
ACAGGCAGTC CAGTGCCCAC CATTCTGTGG AGAAAGGATG GAGTCCTCGT TTCAACCCAA 
GACTCTCGAA TCAAACAGTT GGAGAATGGA GTACTGCAGA TCCGATATGC TAAGCTGGGT 
GATACTGGTC GGTACACCTG CATTGCATCA ACCCCCAGTG GTGAAGCAAC ATGGAGTGCT 
TACATTGAAG TTCAAGAATT TGGAGTTCCA GTTCAGCCTC CAAGACCTAC TGACCCAAAT 
TTAATCCCTA GTGCCCCATC AAAACCTGAA GTGACAGATG TCAGCAGAAA TACAGTCACA 
TTATCATGGC AACCAAATTT GAATTCAGGA GCAACTCCAA CATCTTATAT TATAGAAGCC 
TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACCGTAGCAG AGAATGTGAA AACAGAAACA 
TTTGCCATTA AAGGACTCAA ACCTAATGCA ATTTACCTTT TCCTTGTGAG GGCAGCTAAT 
GCATATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACACA AGATGTCCTA 
CCAACAAGTC AGGGGGTGGA CCACAAGCAG GTCCAGAGAG AGCTGGGAAA TGCTGTTCTG 
CACCTCCACA ACCCCACCGT CCTTTCTTCC TCTTCCATCG AAGTGCACTG GACAGTAGAT 
CAACAGTCTC AGTATATACA AGGATATAAA ATTCTCTATC GGCCATCTGG AGCCAACCAC 
GGAGAATCAG ACTGGTTAGT TTTTGAAGTG AGGACGCCAG CCAAAAACAG TGTGGTTATC 
CCTGATCTCA GAAAGGGAGT CAACTATGAA ATTAAGGCTC GCCCTTTTTT TAATGAATTT 
CAAGGAGCAG ATAGTGAAAT CAAGTTCGCC AAAACCCTGG AAGAAGCACC CAGTGCCCCA 
CCCCAAGGTG TAACTGTATC CAAGAATGAT GGAAACGGAA CTGCAATTCT AGTTAGTTGG 
CAGCCACCTC CAGAAGACAC TCAAAATGGA ATGGTCCAAG AGTATAAGGT TTGGTGTCTG 
GGCAATGAAA CTCGATACCA CATCAACAAA ACAGTGGATG GTTCCACCTT TTCCGTGGTC 
ATTCCCTTTC TTGTTCCTGG AATCCGATAC AGTGTGGAAG TGGCAGCCAG CACTGGGGCT 
GGGTCTGGGG TAAAGAGTGA GCCTCAGTTC ATCCAGCTGG ATGCCCATGG AAACCCTGTG 
TCACCTGAGG ACCAAGTCAG CCTCGCTCAG CAGATTTCAG ATGTGGTGAA GCAGCCGGCC 
TTCATAGCAG GTATTGGAGC AGCCTGTTGG ATCATCCTCA TGGTCTTCAG CATCTGGCTT 
TATCGACACC GCAAGAAGAG AAACGGACTT ACTAGTACTT ACGCGGGTAT CAGAAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTAACT TACCAGAGAG GAGGCGAAGC TGTCAGCAGT 
GGAGGGAGGC CCGGACTTCT CAACATCAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC 
ACGTGGCCTA ATACTGGCAA CAACCACAAT GACTGCTCCA TCAGCTGCTG CACGGCAGGC 
AATGGAAACA GCGACAGCAA CCTCACTACC TACAGTCGCC CAGCTGATTG TATAGCAAAT 
TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT 
GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG 
AAGGATGGGC GTTTTGTCAA TCCATCAGGG CAGCCTACTC CTTATGCCAC CACTCAGCTC 
ATCCAGTCAA ACCTCAGCAA CAACATGAAC AATGGCAGCG GGGACTCTGG CGAGAAGCAC 
TGGAAACCAC TGGGACAGCA GAAACAAGAA GTGGCACCAG TTCAGTACAA CATCATGGAG 
CAAAACAAGC TGAACAAAGG TGAAGAGGCT TATTATCGAG CAAATGACAC AGTTCCTCAA 
ACTATCCCAT ACAACCAATC ATACGACCAG AACACAGGAG GATCCTACAA CAGCTCAGAC 
CGGGGCAGTA GTACATCTGG GAGTCAGGGG CACAAGAAAG GGGCAAGAAC ACCCAAGGTA 
CCAAAACAGG GTGGCATGAA CTGGGCAGAC CTGCTTCCTC CTCCCCCAGC ACATCCTCCT 
CCACACAGCA ATAGCGAAGA GTACAACATT TCTGTAGATG AAAGCTATGA CCAAGAAATG 
CCATGTCCCG TGCCACCAGC AAGGATGTAT TTGCAACAAG ATGAATTAGA AGAGGAGGAA 
GATGAACGAG GCCCCACTCC CCCCGTTCGG GGAGCAGCTT CTTCTCCAGC TGCCGTGTCC 
TATAGCCATC AGTCCACTGC CACTCTGACT CCCTCCCCAC AGGAAGAACT CCAGCCCATG 
TTACAGGATT GTCCAGAGGA GACTGGCCAC ATGCAGCACC AGCCCGACAG GAGACGGCAG 
CCTGTGAGTC CTCCTCCACC ACCACGGCCG ATCTCCCCTC CACATACCTA TGGCTACATT 
TCAGGACCCC TGGTCTCAGA TATGGATACA GATGCGCCAG AAGAGGAAGA AGACGAAGCC 
GACATGGAGG TAGCCAAGAT GCAAACCAGA AGGCTTTTGT TACGTGGGCT TGAGCAGACA 
CCTGCCTCCA GTGTTGGGGA CCTGGAGAGC TCTGTCACGG GGTCCATGAT CAACGGCTGG 
GGCTCAGCCT CAGAGGAGGA CAACATTTCC AGCGGACGCT CCAGTGTTAG TTCTTCGGAT 
GGCTCCTTTT TCACTGATGC TGACTTTGCC CAGGCAGTCG CAGCAGCGGC AGAGTATGCT 
GGTCTGAAAG TGGCACGACG GCAAATGCAG GATGCTGCTG GCCGTCGACA TTTTCATGCG 
TCTCAGTGCC CTAGGCCCAC AAGTCCTGTG TCTACAGACA GCAACATGAG TGCCGCCGTA 
ATGCAGAAAA CCAGACCAGC CAAGAAACCG AAACACCAGC CAGGACATCT GCGCAGAGAA 
ACCTACACAG ATGATCTTCC ACCACCTCCT GTGCCGCCAC CTGCTATAAA GTCACCTACT 
GCCCAATCCA AGACACAGCT GGAAGTACGA CCTGTAGTGG TGCCAAAACT CCCTTCTGTG 
GATGCAAGAA CAGACAGATC ATCAGACAGA AAAGGAAGCA GTTACAAGGG GAGAGAAGTA 
TTGGATGGAA GACAGGTTGT TGACATGCGA ACAAATCCAG GTGATCCCAG AGAAGCACAG 
GAACAGCAAA ATGACGGGAA AGGACGTGGA AACAAGGCAG CAAAACGAGA CCTTCCACCA 
GCAAAGACTC ATCTCATCCA AGAGGATATT CTACCTTATT GTAGACCTAC TTTTCCAACA 
TCAAATAATC CCAGAGATCC CAGTTCCTCA AGCTCAATGT CATCAAGAGG ATCAGGAAGC 
AGACAAAGAG AACAAGCAAA TGTAGGTCGA AGAAATATTG CAGAAATGCA GGTACTTGGA 
GGATATGAAA GAGGAGAAGA TAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796991.1
CDS	407..5320
Translation	MAVLLLMLLLSMAEITSPVGVLDRTKAGSRWKGEKNRSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941502.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941502.1

ORF Insert Sequence:

ATGGCTGTGC TGTTACTGAT GCTGCTGCTC TCGATGGCTG AGATAACTTC TCCTGTGGGT 
GTCTTGGACA GAACGAAAGC TGGGTCAAGG TGGAAAGGTG AAAAGAATCG CTCCCGTCTT 
CGTCAGGAAG ATTTTCCACC TCGCATTGTT GAACACCCTT CAGACCTGAT TGTCTCAAAA 
GGAGAACCTG CAACTTTGAA CTGCAAAGCT GAAGGCCGCC CCACACCCAC TATTGAATGG 
TACAAAGGGG GAGAGAGAGT GGAGACAGAC AAAGATGACC CTCGCTCACA CCGAATGTTG 
CTGCCGAGTG GATCTTTATT TTTCTTACGT ATAGTACATG GACGGAAAAG TAGACCTGAT 
GAAGGAGTCT ATGTCTGTGT AGCAAGGAAT TACCTTGGAG AGGCTGTGAG CCACAATGCA 
TCGCTGGAAG TAGCCATACT TCGGGATGAC TTCAGACAAA ACCCTTCGGA TGTCATGGTT 
GCAGTAGGAG AGCCTGCAGT AATGGAATGC CAACCTCCAC GAGGCCATCC TGAGCCCACC 
ATTTCATGGA AGAAAGATGG CTCTCCACTG GATGATAAAG ATGAAAGAAT AACTATACGA 
GGAGGAAAGC TCATGATCAC TTACACCCGT AAAAGTGACG CTGGCAAATA TGTTTGTGTT 
GGTACCAATA TGGTTGGGGA ACGTGAGAGT GAAGTAGCCG AGCTGACTGT CTTAGAGAGA 
CCATCATTTG TGAAGAGACC CAGTAACTTG GCAGTAACTG TGGATGACAG TGCAGAATTT 
AAATGTGAGG CCCGAGGTGA CCCTGTACCT ACAGTACGAT GGAGGAAAGA TGATGGAGAG 
CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACCT TGAAAATTAG GAAGGTGACA 
GCTGGTGACA TGGGTTCATA CACTTGTGTT GCAGAAAATA TGGTGGGCAA AGCTGAAGCA 
TCTGCTACTC TGACTGTGCA AGTTGAGTCT GAACCTCCAC ATTTTGTTGT GAAACCCCGT 
GACCAGGTTG TTGCTTTGGG ACGGACTGTA ACTTTTCAGT GTGAAGCAAC CGGAAATCCT 
CAACCAGCTA TTTTTTGGAG GAGAGAAGGG AGTCAGAATC TACTTTTCTC ATATCAACCA 
CCACAGTCAT CCAGCCGATT TTCAGTCTCC CAGACTGGCG ACCTCACAAT TACTAATGTC 
CAGCGATCTG ATGTTGGTTA TTACATCTGC CAGACTTTAA ATGTTGCTGG AAGCATCATC 
ACAAAGGCAT ATTTGGAAGT TACAGATGTG ATTGCAGATC GGCCTCCCCC AGTTATTCGA 
CAAGGTCCTG TGAATCAGAC TGTAGCTGTG GATGGCACTT TCGTCCTCAG CTGTGTGGCC 
ACAGGCAGTC CAGTGCCCAC CATTCTGTGG AGAAAGGATG GAGTCCTCGT TTCAACCCAA 
GACTCTCGAA TCAAACAGTT GGAGAATGGA GTACTGCAGA TCCGATATGC TAAGCTGGGT 
GATACTGGTC GGTACACCTG CATTGCATCA ACCCCCAGTG GTGAAGCAAC ATGGAGTGCT 
TACATTGAAG TTCAAGAATT TGGAGTTCCA GTTCAGCCTC CAAGACCTAC TGACCCAAAT 
TTAATCCCTA GTGCCCCATC AAAACCTGAA GTGACAGATG TCAGCAGAAA TACAGTCACA 
TTATCATGGC AACCAAATTT GAATTCAGGA GCAACTCCAA CATCTTATAT TATAGAAGCC 
TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACCGTAGCAG AGAATGTGAA AACAGAAACA 
TTTGCCATTA AAGGACTCAA ACCTAATGCA ATTTACCTTT TCCTTGTGAG GGCAGCTAAT 
GCATATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACACA AGATGTCCTA 
CCAACAAGTC AGGGGGTGGA CCACAAGCAG GTCCAGAGAG AGCTGGGAAA TGCTGTTCTG 
CACCTCCACA ACCCCACCGT CCTTTCTTCC TCTTCCATCG AAGTGCACTG GACAGTAGAT 
CAACAGTCTC AGTATATACA AGGATATAAA ATTCTCTATC GGCCATCTGG AGCCAACCAC 
GGAGAATCAG ACTGGTTAGT TTTTGAAGTG AGGACGCCAG CCAAAAACAG TGTGGTTATC 
CCTGATCTCA GAAAGGGAGT CAACTATGAA ATTAAGGCTC GCCCTTTTTT TAATGAATTT 
CAAGGAGCAG ATAGTGAAAT CAAGTTCGCC AAAACCCTGG AAGAAGCACC CAGTGCCCCA 
CCCCAAGGTG TAACTGTATC CAAGAATGAT GGAAACGGAA CTGCAATTCT AGTTAGTTGG 
CAGCCACCTC CAGAAGACAC TCAAAATGGA ATGGTCCAAG AGTATAAGGT TTGGTGTCTG 
GGCAATGAAA CTCGATACCA CATCAACAAA ACAGTGGATG GTTCCACCTT TTCCGTGGTC 
ATTCCCTTTC TTGTTCCTGG AATCCGATAC AGTGTGGAAG TGGCAGCCAG CACTGGGGCT 
GGGTCTGGGG TAAAGAGTGA GCCTCAGTTC ATCCAGCTGG ATGCCCATGG AAACCCTGTG 
TCACCTGAGG ACCAAGTCAG CCTCGCTCAG CAGATTTCAG ATGTGGTGAA GCAGCCGGCC 
TTCATAGCAG GTATTGGAGC AGCCTGTTGG ATCATCCTCA TGGTCTTCAG CATCTGGCTT 
TATCGACACC GCAAGAAGAG AAACGGACTT ACTAGTACTT ACGCGGGTAT CAGAAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTAACT TACCAGAGAG GAGGCGAAGC TGTCAGCAGT 
GGAGGGAGGC CCGGACTTCT CAACATCAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC 
ACGTGGCCTA ATACTGGCAA CAACCACAAT GACTGCTCCA TCAGCTGCTG CACGGCAGGC 
AATGGAAACA GCGACAGCAA CCTCACTACC TACAGTCGCC CAGCTGATTG TATAGCAAAT 
TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT 
GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG 
AAGGATGGGC GTTTTGTCAA TCCATCAGGG CAGCCTACTC CTTATGCCAC CACTCAGCTC 
ATCCAGTCAA ACCTCAGCAA CAACATGAAC AATGGCAGCG GGGACTCTGG CGAGAAGCAC 
TGGAAACCAC TGGGACAGCA GAAACAAGAA GTGGCACCAG TTCAGTACAA CATCATGGAG 
CAAAACAAGC TGAACAAAGG TGAAGAGGCT TATTATCGAG CAAATGACAC AGTTCCTCAA 
ACTATCCCAT ACAACCAATC ATACGACCAG AACACAGGAG GATCCTACAA CAGCTCAGAC 
CGGGGCAGTA GTACATCTGG GAGTCAGGGG CACAAGAAAG GGGCAAGAAC ACCCAAGGTA 
CCAAAACAGG GTGGCATGAA CTGGGCAGAC CTGCTTCCTC CTCCCCCAGC ACATCCTCCT 
CCACACAGCA ATAGCGAAGA GTACAACATT TCTGTAGATG AAAGCTATGA CCAAGAAATG 
CCATGTCCCG TGCCACCAGC AAGGATGTAT TTGCAACAAG ATGAATTAGA AGAGGAGGAA 
GATGAACGAG GCCCCACTCC CCCCGTTCGG GGAGCAGCTT CTTCTCCAGC TGCCGTGTCC 
TATAGCCATC AGTCCACTGC CACTCTGACT CCCTCCCCAC AGGAAGAACT CCAGCCCATG 
TTACAGGATT GTCCAGAGGA GACTGGCCAC ATGCAGCACC AGCCCGACAG GAGACGGCAG 
CCTGTGAGTC CTCCTCCACC ACCACGGCCG ATCTCCCCTC CACATACCTA TGGCTACATT 
TCAGGACCCC TGGTCTCAGA TATGGATACA GATGCGCCAG AAGAGGAAGA AGACGAAGCC 
GACATGGAGG TAGCCAAGAT GCAAACCAGA AGGCTTTTGT TACGTGGGCT TGAGCAGACA 
CCTGCCTCCA GTGTTGGGGA CCTGGAGAGC TCTGTCACGG GGTCCATGAT CAACGGCTGG 
GGCTCAGCCT CAGAGGAGGA CAACATTTCC AGCGGACGCT CCAGTGTTAG TTCTTCGGAT 
GGCTCCTTTT TCACTGATGC TGACTTTGCC CAGGCAGTCG CAGCAGCGGC AGAGTATGCT 
GGTCTGAAAG TGGCACGACG GCAAATGCAG GATGCTGCTG GCCGTCGACA TTTTCATGCG 
TCTCAGTGCC CTAGGCCCAC AAGTCCTGTG TCTACAGACA GCAACATGAG TGCCGCCGTA 
ATGCAGAAAA CCAGACCAGC CAAGAAACCG AAACACCAGC CAGGACATCT GCGCAGAGAA 
ACCTACACAG ATGATCTTCC ACCACCTCCT GTGCCGCCAC CTGCTATAAA GTCACCTACT 
GCCCAATCCA AGACACAGCT GGAAGTACGA CCTGTAGTGG TGCCAAAACT CCCTTCTGTG 
GATGCAAGAA CAGACAGATC ATCAGACAGA AAAGGAAGCA GTTACAAGGG GAGAGAAGTA 
TTGGATGGAA GACAGGTTGT TGACATGCGA ACAAATCCAG GTGATCCCAG AGAAGCACAG 
GAACAGCAAA ATGACGGGAA AGGACGTGGA AACAAGGCAG CAAAACGAGA CCTTCCACCA 
GCAAAGACTC ATCTCATCCA AGAGGATATT CTACCTTATT GTAGACCTAC TTTTCCAACA 
TCAAATAATC CCAGAGATCC CAGTTCCTCA AGCTCAATGT CATCAAGAGG ATCAGGAAGC 
AGACAAAGAG AACAAGCAAA TGTAGGTCGA AGAAATATTG CAGAAATGCA GGTACTTGGA 
GGATATGAAA GAGGAGAAGA TAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941503.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796992.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

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CloneID	OPc82308
Clone ID Related Accession (Same CDS sequence)	XM_016941503.1
Accession Version	XM_016941503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC ATCAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA TGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA GAGGCTTATT ATCGAGCAAA TGACACAGTT 
CCTCAAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCC GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACAGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGATGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTGGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCTGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACCGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTGTGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796992.1
CDS	1216..6075
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941503.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941503.1

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC ATCAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA TGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA GAGGCTTATT ATCGAGCAAA TGACACAGTT 
CCTCAAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCC GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACAGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGATGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTGGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCTGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACCGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTGTGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941504.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796993.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

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CloneID	OPc82309
Clone ID Related Accession (Same CDS sequence)	XM_016941504.1
Accession Version	XM_016941504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGACG GACTGTAACT TTTCAGTGTG AAGCAACCGG AAATCCTCAA 
CCAGCTATTT TTTGGAGGAG AGAAGGGAGT CAGAATCTAC TTTTCTCATA TCAACCACCA 
CAGTCATCCA GCCGATTTTC AGTCTCCCAG ACTGGCGACC TCACAATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA CATCTGCCAG ACTTTAAATG TTGCTGGAAG CATCATCACA 
AAGGCATATT TGGAAGTTAC AGATGTGATT GCAGATCGGC CTCCCCCAGT TATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCTGTGGAT GGCACTTTCG TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACCAT TCTGTGGAGA AAGGATGGAG TCCTCGTTTC AACCCAAGAC 
TCTCGAATCA AACAGTTGGA GAATGGAGTA CTGCAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AAGCAACATG GAGTGCTTAC 
ATTGAAGTTC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCTACTGA CCCAAATTTA 
ATCCCTAGTG CCCCATCAAA ACCTGAAGTG ACAGATGTCA GCAGAAATAC AGTCACATTA 
TCATGGCAAC CAAATTTGAA TTCAGGAGCA ACTCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC AGAAACATTT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCTAATGCA 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACACAAGA TGTCCTACCA 
ACAAGTCAGG GGGTGGACCA CAAGCAGGTC CAGAGAGAGC TGGGAAATGC TGTTCTGCAC 
CTCCACAACC CCACCGTCCT TTCTTCCTCT TCCATCGAAG TGCACTGGAC AGTAGATCAA 
CAGTCTCAGT ATATACAAGG ATATAAAATT CTCTATCGGC CATCTGGAGC CAACCACGGA 
GAATCAGACT GGTTAGTTTT TGAAGTGAGG ACGCCAGCCA AAAACAGTGT GGTTATCCCT 
GATCTCAGAA AGGGAGTCAA CTATGAAATT AAGGCTCGCC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATCAA GTTCGCCAAA ACCCTGGAAG AAGCACCCAG TGCCCCACCC 
CAAGGTGTAA CTGTATCCAA GAATGATGGA AACGGAACTG CAATTCTAGT TAGTTGGCAG 
CCACCTCCAG AAGACACTCA AAATGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTC GATACCACAT CAACAAAACA GTGGATGGTT CCACCTTTTC CGTGGTCATT 
CCCTTTCTTG TTCCTGGAAT CCGATACAGT GTGGAAGTGG CAGCCAGCAC TGGGGCTGGG 
TCTGGGGTAA AGAGTGAGCC TCAGTTCATC CAGCTGGATG CCCATGGAAA CCCTGTGTCA 
CCTGAGGACC AAGTCAGCCT CGCTCAGCAG ATTTCAGATG TGGTGAAGCA GCCGGCCTTC 
ATAGCAGGTA TTGGAGCAGC CTGTTGGATC ATCCTCATGG TCTTCAGCAT CTGGCTTTAT 
CGACACCGCA AGAAGAGAAA CGGACTTACT AGTACTTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAC CAGAGAGGAG GCGAAGCTGT CAGCAGTGGA 
GGGAGGCCCG GACTTCTCAA CATCAGTGAA CCTGCCACGC AGCCATGGCT GGCAGACACG 
TGGCCTAATA CTGGCAACAA CCACAATGAC TGCTCCATCA GCTGCTGCAC GGCAGGCAAT 
GGAAACAGCG ACAGCAACCT CACTACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCTACTCCTT ATGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGAGCAAAT GACACAGTTC CTCAAACTAT CCCATACAAC 
CAATCATACG ACCAGAACAC AGGAGGATCC TACAACAGCT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AGGGGCACAA GAAAGGGGCA AGAACACCCA AGGTACCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCTCCACA CAGCAATAGC 
GAAGAGTACA ACATTTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG TCCCGTGCCA 
CCAGCAAGGA TGTATTTGCA ACAAGATGAA TTAGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACTCCCCCCG TTCGGGGAGC AGCTTCTTCT CCAGCTGCCG TGTCCTATAG CCATCAGTCC 
ACTGCCACTC TGACTCCCTC CCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCA 
GAGGAGACTG GCCACATGCA GCACCAGCCC GACAGGAGAC GGCAGCCTGT GAGTCCTCCT 
CCACCACCAC GGCCGATCTC CCCTCCACAT ACCTATGGCT ACATTTCAGG ACCCCTGGTC 
TCAGATATGG ATACAGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAGGTAGCC 
AAGATGCAAA CCAGAAGGCT TTTGTTACGT GGGCTTGAGC AGACACCTGC CTCCAGTGTT 
GGGGACCTGG AGAGCTCTGT CACGGGGTCC ATGATCAACG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGT GTTAGTTCTT CGGATGGCTC CTTTTTCACT 
GATGCTGACT TTGCCCAGGC AGTCGCAGCA GCGGCAGAGT ATGCTGGTCT GAAAGTGGCA 
CGACGGCAAA TGCAGGATGC TGCTGGCCGT CGACATTTTC ATGCGTCTCA GTGCCCTAGG 
CCCACAAGTC CTGTGTCTAC AGACAGCAAC ATGAGTGCCG CCGTAATGCA GAAAACCAGA 
CCAGCCAAGA AACCGAAACA CCAGCCAGGA CATCTGCGCA GAGAAACCTA CACAGATGAT 
CTTCCACCAC CTCCTGTGCC GCCACCTGCT ATAAAGTCAC CTACTGCCCA ATCCAAGACA 
CAGCTGGAAG TACGACCTGT AGTGGTGCCA AAACTCCCTT CTGTGGATGC AAGAACAGAC 
AGATCATCAG ACAGAAAAGG AAGCAGTTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAG 
GTTGTTGACA TGCGAACAAA TCCAGGTGAT CCCAGAGAAG CACAGGAACA GCAAAATGAC 
GGGAAAGGAC GTGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTATTGTAGA CCTACTTTTC CAACATCAAA TAATCCCAGA 
GATCCCAGTT CCTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTAC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796993.1
CDS	1..4839
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941504.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941504.1

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGACG GACTGTAACT TTTCAGTGTG AAGCAACCGG AAATCCTCAA 
CCAGCTATTT TTTGGAGGAG AGAAGGGAGT CAGAATCTAC TTTTCTCATA TCAACCACCA 
CAGTCATCCA GCCGATTTTC AGTCTCCCAG ACTGGCGACC TCACAATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA CATCTGCCAG ACTTTAAATG TTGCTGGAAG CATCATCACA 
AAGGCATATT TGGAAGTTAC AGATGTGATT GCAGATCGGC CTCCCCCAGT TATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCTGTGGAT GGCACTTTCG TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACCAT TCTGTGGAGA AAGGATGGAG TCCTCGTTTC AACCCAAGAC 
TCTCGAATCA AACAGTTGGA GAATGGAGTA CTGCAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AAGCAACATG GAGTGCTTAC 
ATTGAAGTTC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCTACTGA CCCAAATTTA 
ATCCCTAGTG CCCCATCAAA ACCTGAAGTG ACAGATGTCA GCAGAAATAC AGTCACATTA 
TCATGGCAAC CAAATTTGAA TTCAGGAGCA ACTCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC AGAAACATTT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCTAATGCA 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACACAAGA TGTCCTACCA 
ACAAGTCAGG GGGTGGACCA CAAGCAGGTC CAGAGAGAGC TGGGAAATGC TGTTCTGCAC 
CTCCACAACC CCACCGTCCT TTCTTCCTCT TCCATCGAAG TGCACTGGAC AGTAGATCAA 
CAGTCTCAGT ATATACAAGG ATATAAAATT CTCTATCGGC CATCTGGAGC CAACCACGGA 
GAATCAGACT GGTTAGTTTT TGAAGTGAGG ACGCCAGCCA AAAACAGTGT GGTTATCCCT 
GATCTCAGAA AGGGAGTCAA CTATGAAATT AAGGCTCGCC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATCAA GTTCGCCAAA ACCCTGGAAG AAGCACCCAG TGCCCCACCC 
CAAGGTGTAA CTGTATCCAA GAATGATGGA AACGGAACTG CAATTCTAGT TAGTTGGCAG 
CCACCTCCAG AAGACACTCA AAATGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTC GATACCACAT CAACAAAACA GTGGATGGTT CCACCTTTTC CGTGGTCATT 
CCCTTTCTTG TTCCTGGAAT CCGATACAGT GTGGAAGTGG CAGCCAGCAC TGGGGCTGGG 
TCTGGGGTAA AGAGTGAGCC TCAGTTCATC CAGCTGGATG CCCATGGAAA CCCTGTGTCA 
CCTGAGGACC AAGTCAGCCT CGCTCAGCAG ATTTCAGATG TGGTGAAGCA GCCGGCCTTC 
ATAGCAGGTA TTGGAGCAGC CTGTTGGATC ATCCTCATGG TCTTCAGCAT CTGGCTTTAT 
CGACACCGCA AGAAGAGAAA CGGACTTACT AGTACTTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAC CAGAGAGGAG GCGAAGCTGT CAGCAGTGGA 
GGGAGGCCCG GACTTCTCAA CATCAGTGAA CCTGCCACGC AGCCATGGCT GGCAGACACG 
TGGCCTAATA CTGGCAACAA CCACAATGAC TGCTCCATCA GCTGCTGCAC GGCAGGCAAT 
GGAAACAGCG ACAGCAACCT CACTACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCTACTCCTT ATGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGAGCAAAT GACACAGTTC CTCAAACTAT CCCATACAAC 
CAATCATACG ACCAGAACAC AGGAGGATCC TACAACAGCT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AGGGGCACAA GAAAGGGGCA AGAACACCCA AGGTACCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCTCCACA CAGCAATAGC 
GAAGAGTACA ACATTTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG TCCCGTGCCA 
CCAGCAAGGA TGTATTTGCA ACAAGATGAA TTAGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACTCCCCCCG TTCGGGGAGC AGCTTCTTCT CCAGCTGCCG TGTCCTATAG CCATCAGTCC 
ACTGCCACTC TGACTCCCTC CCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCA 
GAGGAGACTG GCCACATGCA GCACCAGCCC GACAGGAGAC GGCAGCCTGT GAGTCCTCCT 
CCACCACCAC GGCCGATCTC CCCTCCACAT ACCTATGGCT ACATTTCAGG ACCCCTGGTC 
TCAGATATGG ATACAGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAGGTAGCC 
AAGATGCAAA CCAGAAGGCT TTTGTTACGT GGGCTTGAGC AGACACCTGC CTCCAGTGTT 
GGGGACCTGG AGAGCTCTGT CACGGGGTCC ATGATCAACG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGT GTTAGTTCTT CGGATGGCTC CTTTTTCACT 
GATGCTGACT TTGCCCAGGC AGTCGCAGCA GCGGCAGAGT ATGCTGGTCT GAAAGTGGCA 
CGACGGCAAA TGCAGGATGC TGCTGGCCGT CGACATTTTC ATGCGTCTCA GTGCCCTAGG 
CCCACAAGTC CTGTGTCTAC AGACAGCAAC ATGAGTGCCG CCGTAATGCA GAAAACCAGA 
CCAGCCAAGA AACCGAAACA CCAGCCAGGA CATCTGCGCA GAGAAACCTA CACAGATGAT 
CTTCCACCAC CTCCTGTGCC GCCACCTGCT ATAAAGTCAC CTACTGCCCA ATCCAAGACA 
CAGCTGGAAG TACGACCTGT AGTGGTGCCA AAACTCCCTT CTGTGGATGC AAGAACAGAC 
AGATCATCAG ACAGAAAAGG AAGCAGTTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAG 
GTTGTTGACA TGCGAACAAA TCCAGGTGAT CCCAGAGAAG CACAGGAACA GCAAAATGAC 
GGGAAAGGAC GTGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTATTGTAGA CCTACTTTTC CAACATCAAA TAATCCCAGA 
GATCCCAGTT CCTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTAC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_516592.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_516592.4 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.

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CloneID	OPc82310
Clone ID Related Accession (Same CDS sequence)	XM_516592.5
Accession Version	XM_516592.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_516592.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC CGGACTTCTC 
AACATCAGTG AACCTGCCAC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCTACTCC TTATGCCACC ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGCGG GGACTCTGGC GAGAAGCACT GGAAACCACT GGGACAGCAG 
AAACAAGAAG TGGCACCAGT TCAGTACAAC ATCATGGAGC AAAACAAGCT GAACAAAGAT 
TATCGAGCAA ATGACACAGT TCCTCAAACT ATCCCATACA ACCAATCATA CGACCAGAAC 
ACAGGAGGAT CCTACAACAG CTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAGGGGCAC 
AAGAAAGGGG CAAGAACACC CAAGGTACCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCTCCA CACAGCAATA GCGAAGAGTA CAACATTTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG 
CAACAAGATG AATTAGAAGA GGAGGAAGAT GAACGAGGCC CCACTCCCCC CGTTCGGGGA 
GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT AGCCATCAGT CCACTGCCAC TCTGACTCCC 
TCCCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CAGAGGAGAC TGGCCACATG 
CAGCACCAGC CCGACAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC ACGGCCGATC 
TCCCCTCCAC ATACCTATGG CTACATTTCA GGACCCCTGG TCTCAGATAT GGATACAGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAGGTAG CCAAGATGCA AACCAGAAGG 
CTTTTGTTAC GTGGGCTTGA GCAGACACCT GCCTCCAGTG TTGGGGACCT GGAGAGCTCT 
GTCACGGGGT CCATGATCAA CGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GTGTTAGTTC TTCGGATGGC TCCTTTTTCA CTGATGCTGA CTTTGCCCAG 
GCAGTCGCAG CAGCGGCAGA GTATGCTGGT CTGAAAGTGG CACGACGGCA AATGCAGGAT 
GCTGCTGGCC GTCGACATTT TCATGCGTCT CAGTGCCCTA GGCCCACAAG TCCTGTGTCT 
ACAGACAGCA ACATGAGTGC CGCCGTAATG CAGAAAACCA GACCAGCCAA GAAACCGAAA 
CACCAGCCAG GACATCTGCG CAGAGAAACC TACACAGATG ATCTTCCACC ACCTCCTGTG 
CCGCCACCTG CTATAAAGTC ACCTACTGCC CAATCCAAGA CACAGCTGGA AGTACGACCT 
GTAGTGGTGC CAAAACTCCC TTCTGTGGAT GCAAGAACAG ACAGATCATC AGACAGAAAA 
GGAAGCAGTT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AGGTTGTTGA CATGCGAACA 
AATCCAGGTG ATCCCAGAGA AGCACAGGAA CAGCAAAATG ACGGGAAAGG ACGTGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTATTGTA GACCTACTTT TCCAACATCA AATAATCCCA GAGATCCCAG TTCCTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT ACTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_516592.4
CDS	1008..5828
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_516592.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_516592.5

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC CGGACTTCTC 
AACATCAGTG AACCTGCCAC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCTACTCC TTATGCCACC ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGCGG GGACTCTGGC GAGAAGCACT GGAAACCACT GGGACAGCAG 
AAACAAGAAG TGGCACCAGT TCAGTACAAC ATCATGGAGC AAAACAAGCT GAACAAAGAT 
TATCGAGCAA ATGACACAGT TCCTCAAACT ATCCCATACA ACCAATCATA CGACCAGAAC 
ACAGGAGGAT CCTACAACAG CTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAGGGGCAC 
AAGAAAGGGG CAAGAACACC CAAGGTACCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCTCCA CACAGCAATA GCGAAGAGTA CAACATTTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG 
CAACAAGATG AATTAGAAGA GGAGGAAGAT GAACGAGGCC CCACTCCCCC CGTTCGGGGA 
GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT AGCCATCAGT CCACTGCCAC TCTGACTCCC 
TCCCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CAGAGGAGAC TGGCCACATG 
CAGCACCAGC CCGACAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC ACGGCCGATC 
TCCCCTCCAC ATACCTATGG CTACATTTCA GGACCCCTGG TCTCAGATAT GGATACAGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAGGTAG CCAAGATGCA AACCAGAAGG 
CTTTTGTTAC GTGGGCTTGA GCAGACACCT GCCTCCAGTG TTGGGGACCT GGAGAGCTCT 
GTCACGGGGT CCATGATCAA CGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GTGTTAGTTC TTCGGATGGC TCCTTTTTCA CTGATGCTGA CTTTGCCCAG 
GCAGTCGCAG CAGCGGCAGA GTATGCTGGT CTGAAAGTGG CACGACGGCA AATGCAGGAT 
GCTGCTGGCC GTCGACATTT TCATGCGTCT CAGTGCCCTA GGCCCACAAG TCCTGTGTCT 
ACAGACAGCA ACATGAGTGC CGCCGTAATG CAGAAAACCA GACCAGCCAA GAAACCGAAA 
CACCAGCCAG GACATCTGCG CAGAGAAACC TACACAGATG ATCTTCCACC ACCTCCTGTG 
CCGCCACCTG CTATAAAGTC ACCTACTGCC CAATCCAAGA CACAGCTGGA AGTACGACCT 
GTAGTGGTGC CAAAACTCCC TTCTGTGGAT GCAAGAACAG ACAGATCATC AGACAGAAAA 
GGAAGCAGTT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AGGTTGTTGA CATGCGAACA 
AATCCAGGTG ATCCCAGAGA AGCACAGGAA CAGCAAAATG ACGGGAAAGG ACGTGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTATTGTA GACCTACTTT TCCAACATCA AATAATCCCA GAGATCCCAG TTCCTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT ACTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941506.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796995.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OPc82311
Clone ID Related Accession (Same CDS sequence)	XM_016941506.1
Accession Version	XM_016941506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCCGGACT TCTCAACATC AGTGAACCTG CCACGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGGGCA GCCTACTCCT 
TATGCCACCA CTCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGCGGG 
GACTCTGGCG AGAAGCACTG GAAACCACTG GGACAGCAGA AACAAGAAGT GGCACCAGTT 
CAGTACAACA TCATGGAGCA AAACAAGCTG AACAAAGGTG AAGAGGCTTA TTATCGAGCA 
AATGACACAG TTCCTCAAAC TATCCCATAC AACCAATCAT ACGACCAGAA CACAGGAGGA 
TCCTACAACA GCTCAGACCG GGGCAGTAGT ACATCTGGGA GTCAGGGGCA CAAGAAAGGG 
GCAAGAACAC CCAAGGTACC AAAACAGGGT GGCATGAACT GGGCAGACCT GCTTCCTCCT 
CCCCCAGCAC ATCCTCCTCC ACACAGCAAT AGCGAAGAGT ACAACATTTC TGTAGATGAA 
AGCTATGACC AAGAAATGCC ATGTCCCGTG CCACCAGCAA GGATGTATTT GCAACAAGAT 
GAATTAGAAG AGGAGGAAGA TGAACGAGGC CCCACTCCCC CCGTTCGGGG AGCAGCTTCT 
TCTCCAGCTG CCGTGTCCTA TAGCCATCAG TCCACTGCCA CTCTGACTCC CTCCCCACAG 
GAAGAACTCC AGCCCATGTT ACAGGATTGT CCAGAGGAGA CTGGCCACAT GCAGCACCAG 
CCCGACAGGA GACGGCAGCC TGTGAGTCCT CCTCCACCAC CACGGCCGAT CTCCCCTCCA 
CATACCTATG GCTACATTTC AGGACCCCTG GTCTCAGATA TGGATACAGA TGCGCCAGAA 
GAGGAAGAAG ACGAAGCCGA CATGGAGGTA GCCAAGATGC AAACCAGAAG GCTTTTGTTA 
CGTGGGCTTG AGCAGACACC TGCCTCCAGT GTTGGGGACC TGGAGAGCTC TGTCACGGGG 
TCCATGATCA ACGGCTGGGG CTCAGCCTCA GAGGAGGACA ACATTTCCAG CGGACGCTCC 
AGTGTTAGTT CTTCGGATGG CTCCTTTTTC ACTGATGCTG ACTTTGCCCA GGCAGTCGCA 
GCAGCGGCAG AGTATGCTGG TCTGAAAGTG GCACGACGGC AAATGCAGGA TGCTGCTGGC 
CGTCGACATT TTCATGCGTC TCAGTGCCCT AGGCCCACAA GTCCTGTGTC TACAGACAGC 
AACATGAGTG CCGCCGTAAT GCAGAAAACC AGACCAGCCA AGAAACCGAA ACACCAGCCA 
GGACATCTGC GCAGAGAAAC CTACACAGAT GATCTTCCAC CACCTCCTGT GCCGCCACCT 
GCTATAAAGT CACCTACTGC CCAATCCAAG ACACAGCTGG AAGTACGACC TGTAGTGGTG 
CCAAAACTCC CTTCTGTGGA TGCAAGAACA GACAGATCAT CAGACAGAAA AGGAAGCAGT 
TACAAGGGGA GAGAAGTATT GGATGGAAGA CAGGTTGTTG ACATGCGAAC AAATCCAGGT 
GATCCCAGAG AAGCACAGGA ACAGCAAAAT GACGGGAAAG GACGTGGAAA CAAGGCAGCA 
AAACGAGACC TTCCACCAGC AAAGACTCAT CTCATCCAAG AGGATATTCT ACCTTATTGT 
AGACCTACTT TTCCAACATC AAATAATCCC AGAGATCCCA GTTCCTCAAG CTCAATGTCA 
TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA CAAGCAAATG TAGGTCGAAG AAATATTGCA 
GAAATGCAGG TACTTGGAGG ATATGAAAGA GGAGAAGATA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796995.1
CDS	293..5104
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941506.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941506.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCCGGACT TCTCAACATC AGTGAACCTG CCACGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGGGCA GCCTACTCCT 
TATGCCACCA CTCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGCGGG 
GACTCTGGCG AGAAGCACTG GAAACCACTG GGACAGCAGA AACAAGAAGT GGCACCAGTT 
CAGTACAACA TCATGGAGCA AAACAAGCTG AACAAAGGTG AAGAGGCTTA TTATCGAGCA 
AATGACACAG TTCCTCAAAC TATCCCATAC AACCAATCAT ACGACCAGAA CACAGGAGGA 
TCCTACAACA GCTCAGACCG GGGCAGTAGT ACATCTGGGA GTCAGGGGCA CAAGAAAGGG 
GCAAGAACAC CCAAGGTACC AAAACAGGGT GGCATGAACT GGGCAGACCT GCTTCCTCCT 
CCCCCAGCAC ATCCTCCTCC ACACAGCAAT AGCGAAGAGT ACAACATTTC TGTAGATGAA 
AGCTATGACC AAGAAATGCC ATGTCCCGTG CCACCAGCAA GGATGTATTT GCAACAAGAT 
GAATTAGAAG AGGAGGAAGA TGAACGAGGC CCCACTCCCC CCGTTCGGGG AGCAGCTTCT 
TCTCCAGCTG CCGTGTCCTA TAGCCATCAG TCCACTGCCA CTCTGACTCC CTCCCCACAG 
GAAGAACTCC AGCCCATGTT ACAGGATTGT CCAGAGGAGA CTGGCCACAT GCAGCACCAG 
CCCGACAGGA GACGGCAGCC TGTGAGTCCT CCTCCACCAC CACGGCCGAT CTCCCCTCCA 
CATACCTATG GCTACATTTC AGGACCCCTG GTCTCAGATA TGGATACAGA TGCGCCAGAA 
GAGGAAGAAG ACGAAGCCGA CATGGAGGTA GCCAAGATGC AAACCAGAAG GCTTTTGTTA 
CGTGGGCTTG AGCAGACACC TGCCTCCAGT GTTGGGGACC TGGAGAGCTC TGTCACGGGG 
TCCATGATCA ACGGCTGGGG CTCAGCCTCA GAGGAGGACA ACATTTCCAG CGGACGCTCC 
AGTGTTAGTT CTTCGGATGG CTCCTTTTTC ACTGATGCTG ACTTTGCCCA GGCAGTCGCA 
GCAGCGGCAG AGTATGCTGG TCTGAAAGTG GCACGACGGC AAATGCAGGA TGCTGCTGGC 
CGTCGACATT TTCATGCGTC TCAGTGCCCT AGGCCCACAA GTCCTGTGTC TACAGACAGC 
AACATGAGTG CCGCCGTAAT GCAGAAAACC AGACCAGCCA AGAAACCGAA ACACCAGCCA 
GGACATCTGC GCAGAGAAAC CTACACAGAT GATCTTCCAC CACCTCCTGT GCCGCCACCT 
GCTATAAAGT CACCTACTGC CCAATCCAAG ACACAGCTGG AAGTACGACC TGTAGTGGTG 
CCAAAACTCC CTTCTGTGGA TGCAAGAACA GACAGATCAT CAGACAGAAA AGGAAGCAGT 
TACAAGGGGA GAGAAGTATT GGATGGAAGA CAGGTTGTTG ACATGCGAAC AAATCCAGGT 
GATCCCAGAG AAGCACAGGA ACAGCAAAAT GACGGGAAAG GACGTGGAAA CAAGGCAGCA 
AAACGAGACC TTCCACCAGC AAAGACTCAT CTCATCCAAG AGGATATTCT ACCTTATTGT 
AGACCTACTT TTCCAACATC AAATAATCCC AGAGATCCCA GTTCCTCAAG CTCAATGTCA 
TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA CAAGCAAATG TAGGTCGAAG AAATATTGCA 
GAAATGCAGG TACTTGGAGG ATATGAAAGA GGAGAAGATA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941507.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796996.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X12, mRNA.

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CloneID	OPc82312
Clone ID Related Accession (Same CDS sequence)	XM_016941507.1
Accession Version	XM_016941507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGG GCAGCCTACT CCTTATGCCA CCACTCAGCT CATCCAGTCA 
AACCTCAGCA ACAACATGAA CAATGGCAGC GGGGACTCTG GCGAGAAGCA CTGGAAACCA 
CTGGGACAGC AGAAACAAGA AGTGGCACCA GTTCAGTACA ACATCATGGA GCAAAACAAG 
CTGAACAAAG ATTATCGAGC AAATGACACA GTTCCTCAAA CTATCCCATA CAACCAATCA 
TACGACCAGA ACACAGGAGG ATCCTACAAC AGCTCAGACC GGGGCAGTAG TACATCTGGG 
AGTCAGGGGC ACAAGAAAGG GGCAAGAACA CCCAAGGTAC CAAAACAGGG TGGCATGAAC 
TGGGCAGACC TGCTTCCTCC TCCCCCAGCA CATCCTCCTC CACACAGCAA TAGCGAAGAG 
TACAACATTT CTGTAGATGA AAGCTATGAC CAAGAAATGC CATGTCCCGT GCCACCAGCA 
AGGATGTATT TGCAACAAGA TGAATTAGAA GAGGAGGAAG ATGAACGAGG CCCCACTCCC 
CCCGTTCGGG GAGCAGCTTC TTCTCCAGCT GCCGTGTCCT ATAGCCATCA GTCCACTGCC 
ACTCTGACTC CCTCCCCACA GGAAGAACTC CAGCCCATGT TACAGGATTG TCCAGAGGAG 
ACTGGCCACA TGCAGCACCA GCCCGACAGG AGACGGCAGC CTGTGAGTCC TCCTCCACCA 
CCACGGCCGA TCTCCCCTCC ACATACCTAT GGCTACATTT CAGGACCCCT GGTCTCAGAT 
ATGGATACAG ATGCGCCAGA AGAGGAAGAA GACGAAGCCG ACATGGAGGT AGCCAAGATG 
CAAACCAGAA GGCTTTTGTT ACGTGGGCTT GAGCAGACAC CTGCCTCCAG TGTTGGGGAC 
CTGGAGAGCT CTGTCACGGG GTCCATGATC AACGGCTGGG GCTCAGCCTC AGAGGAGGAC 
AACATTTCCA GCGGACGCTC CAGTGTTAGT TCTTCGGATG GCTCCTTTTT CACTGATGCT 
GACTTTGCCC AGGCAGTCGC AGCAGCGGCA GAGTATGCTG GTCTGAAAGT GGCACGACGG 
CAAATGCAGG ATGCTGCTGG CCGTCGACAT TTTCATGCGT CTCAGTGCCC TAGGCCCACA 
AGTCCTGTGT CTACAGACAG CAACATGAGT GCCGCCGTAA TGCAGAAAAC CAGACCAGCC 
AAGAAACCGA AACACCAGCC AGGACATCTG CGCAGAGAAA CCTACACAGA TGATCTTCCA 
CCACCTCCTG TGCCGCCACC TGCTATAAAG TCACCTACTG CCCAATCCAA GACACAGCTG 
GAAGTACGAC CTGTAGTGGT GCCAAAACTC CCTTCTGTGG ATGCAAGAAC AGACAGATCA 
TCAGACAGAA AAGGAAGCAG TTACAAGGGG AGAGAAGTAT TGGATGGAAG ACAGGTTGTT 
GACATGCGAA CAAATCCAGG TGATCCCAGA GAAGCACAGG AACAGCAAAA TGACGGGAAA 
GGACGTGGAA ACAAGGCAGC AAAACGAGAC CTTCCACCAG CAAAGACTCA TCTCATCCAA 
GAGGATATTC TACCTTATTG TAGACCTACT TTTCCAACAT CAAATAATCC CAGAGATCCC 
AGTTCCTCAA GCTCAATGTC ATCAAGAGGA TCAGGAAGCA GACAAAGAGA ACAAGCAAAT 
GTAGGTCGAA GAAATATTGC AGAAATGCAG GTACTTGGAG GATATGAAAG AGGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796996.1
CDS	293..5065
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941507.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941507.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT GCAAGTTGAG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTTTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCT GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCAT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTC GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CTTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGG GCAGCCTACT CCTTATGCCA CCACTCAGCT CATCCAGTCA 
AACCTCAGCA ACAACATGAA CAATGGCAGC GGGGACTCTG GCGAGAAGCA CTGGAAACCA 
CTGGGACAGC AGAAACAAGA AGTGGCACCA GTTCAGTACA ACATCATGGA GCAAAACAAG 
CTGAACAAAG ATTATCGAGC AAATGACACA GTTCCTCAAA CTATCCCATA CAACCAATCA 
TACGACCAGA ACACAGGAGG ATCCTACAAC AGCTCAGACC GGGGCAGTAG TACATCTGGG 
AGTCAGGGGC ACAAGAAAGG GGCAAGAACA CCCAAGGTAC CAAAACAGGG TGGCATGAAC 
TGGGCAGACC TGCTTCCTCC TCCCCCAGCA CATCCTCCTC CACACAGCAA TAGCGAAGAG 
TACAACATTT CTGTAGATGA AAGCTATGAC CAAGAAATGC CATGTCCCGT GCCACCAGCA 
AGGATGTATT TGCAACAAGA TGAATTAGAA GAGGAGGAAG ATGAACGAGG CCCCACTCCC 
CCCGTTCGGG GAGCAGCTTC TTCTCCAGCT GCCGTGTCCT ATAGCCATCA GTCCACTGCC 
ACTCTGACTC CCTCCCCACA GGAAGAACTC CAGCCCATGT TACAGGATTG TCCAGAGGAG 
ACTGGCCACA TGCAGCACCA GCCCGACAGG AGACGGCAGC CTGTGAGTCC TCCTCCACCA 
CCACGGCCGA TCTCCCCTCC ACATACCTAT GGCTACATTT CAGGACCCCT GGTCTCAGAT 
ATGGATACAG ATGCGCCAGA AGAGGAAGAA GACGAAGCCG ACATGGAGGT AGCCAAGATG 
CAAACCAGAA GGCTTTTGTT ACGTGGGCTT GAGCAGACAC CTGCCTCCAG TGTTGGGGAC 
CTGGAGAGCT CTGTCACGGG GTCCATGATC AACGGCTGGG GCTCAGCCTC AGAGGAGGAC 
AACATTTCCA GCGGACGCTC CAGTGTTAGT TCTTCGGATG GCTCCTTTTT CACTGATGCT 
GACTTTGCCC AGGCAGTCGC AGCAGCGGCA GAGTATGCTG GTCTGAAAGT GGCACGACGG 
CAAATGCAGG ATGCTGCTGG CCGTCGACAT TTTCATGCGT CTCAGTGCCC TAGGCCCACA 
AGTCCTGTGT CTACAGACAG CAACATGAGT GCCGCCGTAA TGCAGAAAAC CAGACCAGCC 
AAGAAACCGA AACACCAGCC AGGACATCTG CGCAGAGAAA CCTACACAGA TGATCTTCCA 
CCACCTCCTG TGCCGCCACC TGCTATAAAG TCACCTACTG CCCAATCCAA GACACAGCTG 
GAAGTACGAC CTGTAGTGGT GCCAAAACTC CCTTCTGTGG ATGCAAGAAC AGACAGATCA 
TCAGACAGAA AAGGAAGCAG TTACAAGGGG AGAGAAGTAT TGGATGGAAG ACAGGTTGTT 
GACATGCGAA CAAATCCAGG TGATCCCAGA GAAGCACAGG AACAGCAAAA TGACGGGAAA 
GGACGTGGAA ACAAGGCAGC AAAACGAGAC CTTCCACCAG CAAAGACTCA TCTCATCCAA 
GAGGATATTC TACCTTATTG TAGACCTACT TTTCCAACAT CAAATAATCC CAGAGATCCC 
AGTTCCTCAA GCTCAATGTC ATCAAGAGGA TCAGGAAGCA GACAAAGAGA ACAAGCAAAT 
GTAGGTCGAA GAAATATTGC AGAAATGCAG GTACTTGGAG GATATGAAAG AGGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_003309895.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003309943.2 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X13, mRNA.

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CloneID	OPc82313
Clone ID Related Accession (Same CDS sequence)	XM_003309895.3
Accession Version	XM_003309895.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_003309895.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC CGGACTTCTC 
AACATCAGTG AACCTGCCAC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003309943.2
CDS	1008..5663
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKDYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_003309895.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003309895.3

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC CGGACTTCTC 
AACATCAGTG AACCTGCCAC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGGGCAGCCT ACTCCTTATG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCAT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC AAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCCGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CAGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ATGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTGGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCTG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC CGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTG TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TATTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_016941508.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796997.1 Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X14, mRNA.

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CloneID	OPc82314
Clone ID Related Accession (Same CDS sequence)	XM_016941508.1
Accession Version	XM_016941508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	roundabout homolog 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC ATCAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA TGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA GAGGCTTATT ATCGAGCAAA TGACACAGTT 
CCTCAAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCC GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACAGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGATGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTGGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCTGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACCGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTGTGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796997.1
CDS	205..4824
Translation	MLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVESEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIMEQNKLNKGEEAYYRANDTVPQTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKPKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSVDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_016941508.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes roundabout guidance receptor 1 (ROBO1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016941508.1

ORF Insert Sequence:

ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTGCAAGTT GAGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT TTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCTGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CATGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTTATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTCGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCCGG ACTTCTCAAC ATCAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA TGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGGTGAA GAGGCTTATT ATCGAGCAAA TGACACAGTT 
CCTCAAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCC GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACAGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGATGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTGGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCTGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACCGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTGTGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ROBO1
Entrez Gene ID	460518
Full Name	roundabout guidance receptor 1
General protein information	Preferred Names roundabout guidance receptor 1 Names roundabout homolog 1 roundabout 1 roundabout, axon guidance receptor, homolog 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010242	XP_554155.3
Danio rerio (zebrafish)	robo1	NP_571556.1
Drosophila melanogaster (fruit fly)	robo3	NP_001259866.1
Homo sapiens (human)	ROBO1	NP_002932.1
Pan troglodytes (chimpanzee)	ROBO1	XP_516592.3
Gallus gallus (chicken)	ROBO1	XP_004938403.1
Caenorhabditis elegans (roundworm)	sax-3	NP_001024990.1
Xenopus tropicalis (tropical clawed frog)	robo1	NP_001096171.1
	ROBO1	XP_002802665.1
Canis lupus familiaris (dog)	ROBO1	XP_544814.4
Bos taurus (cattle)	ROBO1	NP_001179817.2
Mus musculus (house mouse)	Robo1	NP_062286.2
Rattus norvegicus (Norway rat)	Robo1	NP_071524.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ROBO1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

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