Robo1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006522960.3	XP_006523023.1	roundabout homolog 1 isoform X5
XM_006522963.3	XP_006523026.1	roundabout homolog 1 isoform X9
XM_030249003.1	XP_030104863.1	roundabout homolog 1 isoform X10
XM_011246106.2	XP_011244408.1	roundabout homolog 1 isoform X12
NM_019413.2	NP_062286.2	roundabout homolog 1 precursor
XM_017316894.1	XP_017172383.1	roundabout homolog 1 isoform X4
XM_006522958.2	XP_006523021.1	roundabout homolog 1 isoform X3
XM_006522960.4	XP_006523023.1	roundabout homolog 1 isoform X5
XM_006522961.2	XP_006523024.1	roundabout homolog 1 isoform X7
XM_017316893.1	XP_017172382.1	roundabout homolog 1 isoform X1
XM_006522962.4	XP_006523025.1	roundabout homolog 1 isoform X8
XM_006522964.4	XP_006523027.1	roundabout homolog 1 isoform X11
XM_006522965.1	XP_006523028.1	roundabout homolog 1 isoform X12
XM_017316894.2	XP_017172383.1	roundabout homolog 1 isoform X4
XM_011246105.2	XP_011244407.1	roundabout homolog 1 isoform X6
XM_006522962.3	XP_006523025.1	roundabout homolog 1 isoform X8
XM_006522964.3	XP_006523027.1	roundabout homolog 1 isoform X10
XM_006522957.2	XP_006523020.1	roundabout homolog 1 isoform X2
XM_006522961.3	XP_006523024.1	roundabout homolog 1 isoform X7
XM_006522963.4	XP_006523026.1	roundabout homolog 1 isoform X9
XM_006522965.2	XP_006523028.1	roundabout homolog 1 isoform X13
XM_030249005.1	XP_030104865.1	roundabout homolog 1 isoform X14

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu52700	XM_006522960.4 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52701	XM_006522961.3 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52702	XM_006522962.4 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52703	XM_006522963.4 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52704	XM_006522964.4 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52705	XM_006522965.2 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79277	XM_017316893.1 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79278	XM_017316894.2 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu86897	XM_030249003.1 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu86898	XM_030249005.1 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu22089	NM_019413.2 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu52697	XM_006522957.2 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu52698	XM_006522958.2 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu68623	XM_011246105.2 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu68624	XM_011246106.2 Latest version!	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu52700	XM_006522960.3	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu79278	XM_017316894.1	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52702	XM_006522962.3	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52704	XM_006522964.3	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52701	XM_006522961.2	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52703	XM_006522963.3	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52705	XM_006522965.1	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

Robo1 ( XM_006522960.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523023.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu52700
Clone ID Related Accession (Same CDS sequence)	XM_006522960.3 , XM_006522960.4
Accession Version	XM_006522960.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522960.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG ACCCTGAGGA CCTAGAGAGG 
GGGAACGACA ACGGAACGCC AGCCCCCACC TCTGATAACG ATGACAATTC GCTGGGCTAC 
ACAGGCTCCC GTCTTCGTCA GGAAGATTTT CCACCCCGCA TTGTTGAACA CCCTTCAGAC 
CTGATCGTCT CCAAAGGAGA ACCTGCCACT CTGAACTGTA AAGCCGAAGG CCGCCCCACC 
CCGACGATAG AATGGTACAA AGGTGGGGAA AGAGTGGAGA CGGACAAAGA TGACCCGCGC 
TCACACCGGA TGCTGCTGCC AAGCGGGTCT TTATTTTTCT TACGCATAGT CCATGGACGG 
AAGAGTAGGC CCGACGAAGG GGTCTACATC TGTGTGGCTA GGAATTACCT CGGAGAGGCT 
GTGAGCCACA ACGCATCGCT GGAGGTAGCT ATACTACGGG ATGACTTCAG ACAAAACCCT 
TCAGATGTCA TGGTGGCAGT AGGCGAACCT GCAGTCATGG AATGCCAGCC TCCACGGGGC 
CATCCAGAGC CCACCATCTC CTGGAAAAAG GATGGCTCAC CCCTGGATGA TAAAGATGAA 
AGAATCACTA TACGAGGAGG AAAACTCATG ATCACTTATA CGCGTAAAAG CGATGCTGGC 
AAGTATGTTT GCGTTGGCAC CAACATGGTG GGGGAACGGG AGAGTGAAGT CGCAGAGCTA 
ACCGTTTTAG AGAGACCATC GTTTGTGAAG AGACCCAGTA ATTTGGCAGT GACTGTGGAT 
GACAGTGCAG AATTTAAATG TGAGGCCCGA GGAGACCCTG TGCCTACAGT TCGATGGAGG 
AAAGATGACG GAGAGCTGCC CAAATCCAGA TATGAAATCC GAGATGATCA TACTTTGAAA 
ATTCGGAAGG TGACTGCTGG CGACATGGGA TCATACACTT GTGTGGCAGA AAATATGGTC 
GGGAAAGCTG AAGCTTCCGC TACTTTGACA GTTCAAGTTG GGTCTGAGCC GCCACATTTC 
GTTGTAAAAC CTCGGGACCA GGTGGTTGCC TTGGGAAGAA CTGTGACGTT TCAGTGCGAA 
GCAACTGGAA ATCCTCAACC AGCTATCTTT TGGAGGAGAG AGGGGAGTCA GAATCTACTT 
TTCTCCTACC AGCCTCCACA GTCCTCTAGC CGATTTTCAG TTTCGCAGAC AGGAGACCTC 
ACCATTACGA ACGTCCAGCG GTCTGATGTC GGCTACTACA TCTGTCAGAC TTTAAATGTT 
GCTGGAAGTA TCATCACAAA GGCCTATTTG GAAGTTACTG ATGTGATTGC AGACCGGCCT 
CCCCCAGTCA TTCGGCAAGG TCCTGTGAAT CAGACTGTAG CAGTGGATGG CACTTTAATC 
CTCAGCTGTG TGGCCACAGG CAGTCCAGCA CCCACAATTC TGTGGAGGAA GGATGGAGTC 
CTTGTTTCAA CCCAAGATTC TCGGATCAAA CAGCTGGAGA GTGGCGTGCT GCAGATCCGC 
TACGCTAAGC TGGGTGACAC GGGTCGGTAC ACCTGCACCG CATCGACCCC CAGCGGTGAG 
GCCACGTGGA GCGCTTACAT CGAAGTCCAG GAATTTGGGG TTCCAGTTCA ACCCCCAAGA 
CCCACCGACC CCAACTTAAT CCCCAGCGCC CCCTCAAAGC CCGAAGTTAC AGATGTCAGC 
AAAAACACGG TCACTTTATC GTGGCAGCCA AACTTGAACT CAGGAGCAAC CCCAACATCT 
TATATTATAG AAGCCTTCAG CCATGCATCT GGTAGCAGCT GGCAGACGGC GGCAGAAAAT 
GTGAAAACAG AAACATTTGC CATCAAAGGA CTCAAACCTA ACGCCATTTA CCTTTTCCTG 
GTGAGGGCAG CAAATGCCTA TGGAATTAGT GATCCAAGCC AAATATCAGA TCCAGTGAAA 
ACGCAAGATG TCCCACCAAC CAGCCAAGGG GTGGACCACA AGCAGGTCCA AAGAGAACTG 
GGGAATGTTG TCTTACATCT GCACAACCCG ACCATCCTTT CTTCCTCCTC AGTCGAAGTG 
CACTGGACGG TGGACCAACA ATCTCAATAT ATTCAAGGCT ATAAAATTCT CTACCGCCCA 
TCTGGAGCCA GCCATGGTGA ATCGGAGTGG TTAGTTTTTG AAGTGAGGAC GCCAACGAAA 
AACAGCGTGG TGATCCCCGA TCTCAGAAAA GGCGTCAACT ACGAGATTAA GGCTCGCCCA 
TTTTTTAATG AGTTTCAAGG AGCAGACAGT GAAATCAAAT TTGCCAAAAC CTTAGAAGAA 
GCACCAAGTG CCCCTCCCCG AAGTGTAACT GTATCCAAGA ATGATGGAAA TGGGACTGCA 
ATCCTGGTCA CTTGGCAACC GCCTCCTGAA GACACACAAA ATGGAATGGT CCAAGAATAT 
AAGGTTTGGT GTCTCGGTAA TGAAACGAAG TATCACATAA ACAAGACAGT CGATGGCTCG 
ACCTTCTCGG TGGTCATCCC CTCTCTGGTT CCTGGGATCC GATACAGTGT GGAGGTGGCA 
GCGAGCACTG GGGCGGGTCC CGGGGTGAAG AGCGAACCTC AGTTCATCCA GTTAGATTCT 
CACGGAAACC CCGTGTCTCC CGAGGACCAA GTCAGCCTTG CTCAGCAGAT CTCCGACGTG 
GTGAGGCAGC CGGCGTTCAT TGCAGGCATT GGGGCAGCCT GTTGGATTAT CCTCATGGTC 
TTCAGCATCT GGCTTTACAG ACACCGCAAG AAGAGAAACG GACTCACCAG CACGTATGCG 
GGTATCCGGA AAGTCCCGTC CTTTACCTTC ACGCCAACAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT 
GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT 
GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC 
TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG 
TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT 
CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA 
GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG 
GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT 
CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA 
ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG 
GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG 
TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC 
ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG 
CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC 
ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA 
GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG 
ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC 
TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC 
GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT 
GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT 
GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT 
GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG 
GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC 
ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT 
ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA 
GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA 
CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA 
CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA 
ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG 
AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT 
GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523023.1
CDS	426..5402
Translation	MGGIKMKWKHLPLLVISLLTLSRKQLLLAQLIPDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522960.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522960.4

ORF Insert Sequence:

ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG ACCCTGAGGA CCTAGAGAGG 
GGGAACGACA ACGGAACGCC AGCCCCCACC TCTGATAACG ATGACAATTC GCTGGGCTAC 
ACAGGCTCCC GTCTTCGTCA GGAAGATTTT CCACCCCGCA TTGTTGAACA CCCTTCAGAC 
CTGATCGTCT CCAAAGGAGA ACCTGCCACT CTGAACTGTA AAGCCGAAGG CCGCCCCACC 
CCGACGATAG AATGGTACAA AGGTGGGGAA AGAGTGGAGA CGGACAAAGA TGACCCGCGC 
TCACACCGGA TGCTGCTGCC AAGCGGGTCT TTATTTTTCT TACGCATAGT CCATGGACGG 
AAGAGTAGGC CCGACGAAGG GGTCTACATC TGTGTGGCTA GGAATTACCT CGGAGAGGCT 
GTGAGCCACA ACGCATCGCT GGAGGTAGCT ATACTACGGG ATGACTTCAG ACAAAACCCT 
TCAGATGTCA TGGTGGCAGT AGGCGAACCT GCAGTCATGG AATGCCAGCC TCCACGGGGC 
CATCCAGAGC CCACCATCTC CTGGAAAAAG GATGGCTCAC CCCTGGATGA TAAAGATGAA 
AGAATCACTA TACGAGGAGG AAAACTCATG ATCACTTATA CGCGTAAAAG CGATGCTGGC 
AAGTATGTTT GCGTTGGCAC CAACATGGTG GGGGAACGGG AGAGTGAAGT CGCAGAGCTA 
ACCGTTTTAG AGAGACCATC GTTTGTGAAG AGACCCAGTA ATTTGGCAGT GACTGTGGAT 
GACAGTGCAG AATTTAAATG TGAGGCCCGA GGAGACCCTG TGCCTACAGT TCGATGGAGG 
AAAGATGACG GAGAGCTGCC CAAATCCAGA TATGAAATCC GAGATGATCA TACTTTGAAA 
ATTCGGAAGG TGACTGCTGG CGACATGGGA TCATACACTT GTGTGGCAGA AAATATGGTC 
GGGAAAGCTG AAGCTTCCGC TACTTTGACA GTTCAAGTTG GGTCTGAGCC GCCACATTTC 
GTTGTAAAAC CTCGGGACCA GGTGGTTGCC TTGGGAAGAA CTGTGACGTT TCAGTGCGAA 
GCAACTGGAA ATCCTCAACC AGCTATCTTT TGGAGGAGAG AGGGGAGTCA GAATCTACTT 
TTCTCCTACC AGCCTCCACA GTCCTCTAGC CGATTTTCAG TTTCGCAGAC AGGAGACCTC 
ACCATTACGA ACGTCCAGCG GTCTGATGTC GGCTACTACA TCTGTCAGAC TTTAAATGTT 
GCTGGAAGTA TCATCACAAA GGCCTATTTG GAAGTTACTG ATGTGATTGC AGACCGGCCT 
CCCCCAGTCA TTCGGCAAGG TCCTGTGAAT CAGACTGTAG CAGTGGATGG CACTTTAATC 
CTCAGCTGTG TGGCCACAGG CAGTCCAGCA CCCACAATTC TGTGGAGGAA GGATGGAGTC 
CTTGTTTCAA CCCAAGATTC TCGGATCAAA CAGCTGGAGA GTGGCGTGCT GCAGATCCGC 
TACGCTAAGC TGGGTGACAC GGGTCGGTAC ACCTGCACCG CATCGACCCC CAGCGGTGAG 
GCCACGTGGA GCGCTTACAT CGAAGTCCAG GAATTTGGGG TTCCAGTTCA ACCCCCAAGA 
CCCACCGACC CCAACTTAAT CCCCAGCGCC CCCTCAAAGC CCGAAGTTAC AGATGTCAGC 
AAAAACACGG TCACTTTATC GTGGCAGCCA AACTTGAACT CAGGAGCAAC CCCAACATCT 
TATATTATAG AAGCCTTCAG CCATGCATCT GGTAGCAGCT GGCAGACGGC GGCAGAAAAT 
GTGAAAACAG AAACATTTGC CATCAAAGGA CTCAAACCTA ACGCCATTTA CCTTTTCCTG 
GTGAGGGCAG CAAATGCCTA TGGAATTAGT GATCCAAGCC AAATATCAGA TCCAGTGAAA 
ACGCAAGATG TCCCACCAAC CAGCCAAGGG GTGGACCACA AGCAGGTCCA AAGAGAACTG 
GGGAATGTTG TCTTACATCT GCACAACCCG ACCATCCTTT CTTCCTCCTC AGTCGAAGTG 
CACTGGACGG TGGACCAACA ATCTCAATAT ATTCAAGGCT ATAAAATTCT CTACCGCCCA 
TCTGGAGCCA GCCATGGTGA ATCGGAGTGG TTAGTTTTTG AAGTGAGGAC GCCAACGAAA 
AACAGCGTGG TGATCCCCGA TCTCAGAAAA GGCGTCAACT ACGAGATTAA GGCTCGCCCA 
TTTTTTAATG AGTTTCAAGG AGCAGACAGT GAAATCAAAT TTGCCAAAAC CTTAGAAGAA 
GCACCAAGTG CCCCTCCCCG AAGTGTAACT GTATCCAAGA ATGATGGAAA TGGGACTGCA 
ATCCTGGTCA CTTGGCAACC GCCTCCTGAA GACACACAAA ATGGAATGGT CCAAGAATAT 
AAGGTTTGGT GTCTCGGTAA TGAAACGAAG TATCACATAA ACAAGACAGT CGATGGCTCG 
ACCTTCTCGG TGGTCATCCC CTCTCTGGTT CCTGGGATCC GATACAGTGT GGAGGTGGCA 
GCGAGCACTG GGGCGGGTCC CGGGGTGAAG AGCGAACCTC AGTTCATCCA GTTAGATTCT 
CACGGAAACC CCGTGTCTCC CGAGGACCAA GTCAGCCTTG CTCAGCAGAT CTCCGACGTG 
GTGAGGCAGC CGGCGTTCAT TGCAGGCATT GGGGCAGCCT GTTGGATTAT CCTCATGGTC 
TTCAGCATCT GGCTTTACAG ACACCGCAAG AAGAGAAACG GACTCACCAG CACGTATGCG 
GGTATCCGGA AAGTCCCGTC CTTTACCTTC ACGCCAACAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT 
GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT 
GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC 
TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG 
TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT 
CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA 
GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG 
GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT 
CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA 
ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG 
GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG 
TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC 
ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG 
CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC 
ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA 
GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG 
ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC 
TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC 
GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT 
GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT 
GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT 
GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG 
GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC 
ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT 
ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA 
GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA 
CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA 
CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA 
ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG 
AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT 
GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522961.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523024.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA.

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CloneID	OMu52701
Clone ID Related Accession (Same CDS sequence)	XM_006522961.2 , XM_006522961.3
Accession Version	XM_006522961.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT 
CGTCAGGAAG ATTTTCCACC CCGCATTGTT GAACACCCTT CAGACCTGAT CGTCTCCAAA 
GGAGAACCTG CCACTCTGAA CTGTAAAGCC GAAGGCCGCC CCACCCCGAC GATAGAATGG 
TACAAAGGTG GGGAAAGAGT GGAGACGGAC AAAGATGACC CGCGCTCACA CCGGATGCTG 
CTGCCAAGCG GGTCTTTATT TTTCTTACGC ATAGTCCATG GACGGAAGAG TAGGCCCGAC 
GAAGGGGTCT ACATCTGTGT GGCTAGGAAT TACCTCGGAG AGGCTGTGAG CCACAACGCA 
TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTCATGGTG 
GCAGTAGGCG AACCTGCAGT CATGGAATGC CAGCCTCCAC GGGGCCATCC AGAGCCCACC 
ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ATGAAAGAAT CACTATACGA 
GGAGGAAAAC TCATGATCAC TTATACGCGT AAAAGCGATG CTGGCAAGTA TGTTTGCGTT 
GGCACCAACA TGGTGGGGGA ACGGGAGAGT GAAGTCGCAG AGCTAACCGT TTTAGAGAGA 
CCATCGTTTG TGAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCAGAATTT 
AAATGTGAGG CCCGAGGAGA CCCTGTGCCT ACAGTTCGAT GGAGGAAAGA TGACGGAGAG 
CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACT 
GCTGGCGACA TGGGATCATA CACTTGTGTG GCAGAAAATA TGGTCGGGAA AGCTGAAGCT 
TCCGCTACTT TGACAGTTCA AGTTGGGTCT GAGCCGCCAC ATTTCGTTGT AAAACCTCGG 
GACCAGGTGG TTGCCTTGGG AAGAACTGTG ACGTTTCAGT GCGAAGCAAC TGGAAATCCT 
CAACCAGCTA TCTTTTGGAG GAGAGAGGGG AGTCAGAATC TACTTTTCTC CTACCAGCCT 
CCACAGTCCT CTAGCCGATT TTCAGTTTCG CAGACAGGAG ACCTCACCAT TACGAACGTC 
CAGCGGTCTG ATGTCGGCTA CTACATCTGT CAGACTTTAA ATGTTGCTGG AAGTATCATC 
ACAAAGGCCT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGG 
CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGCACTT TAATCCTCAG CTGTGTGGCC 
ACAGGCAGTC CAGCACCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA 
GATTCTCGGA TCAAACAGCT GGAGAGTGGC GTGCTGCAGA TCCGCTACGC TAAGCTGGGT 
GACACGGGTC GGTACACCTG CACCGCATCG ACCCCCAGCG GTGAGGCCAC GTGGAGCGCT 
TACATCGAAG TCCAGGAATT TGGGGTTCCA GTTCAACCCC CAAGACCCAC CGACCCCAAC 
TTAATCCCCA GCGCCCCCTC AAAGCCCGAA GTTACAGATG TCAGCAAAAA CACGGTCACT 
TTATCGTGGC AGCCAAACTT GAACTCAGGA GCAACCCCAA CATCTTATAT TATAGAAGCC 
TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACGGCGGCAG AAAATGTGAA AACAGAAACA 
TTTGCCATCA AAGGACTCAA ACCTAACGCC ATTTACCTTT TCCTGGTGAG GGCAGCAAAT 
GCCTATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACGCA AGATGTCCCA 
CCAACCAGCC AAGGGGTGGA CCACAAGCAG GTCCAAAGAG AACTGGGGAA TGTTGTCTTA 
CATCTGCACA ACCCGACCAT CCTTTCTTCC TCCTCAGTCG AAGTGCACTG GACGGTGGAC 
CAACAATCTC AATATATTCA AGGCTATAAA ATTCTCTACC GCCCATCTGG AGCCAGCCAT 
GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACGCCAA CGAAAAACAG CGTGGTGATC 
CCCGATCTCA GAAAAGGCGT CAACTACGAG ATTAAGGCTC GCCCATTTTT TAATGAGTTT 
CAAGGAGCAG ACAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT 
CCCCGAAGTG TAACTGTATC CAAGAATGAT GGAAATGGGA CTGCAATCCT GGTCACTTGG 
CAACCGCCTC CTGAAGACAC ACAAAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC 
GGTAATGAAA CGAAGTATCA CATAAACAAG ACAGTCGATG GCTCGACCTT CTCGGTGGTC 
ATCCCCTCTC TGGTTCCTGG GATCCGATAC AGTGTGGAGG TGGCAGCGAG CACTGGGGCG 
GGTCCCGGGG TGAAGAGCGA ACCTCAGTTC ATCCAGTTAG ATTCTCACGG AAACCCCGTG 
TCTCCCGAGG ACCAAGTCAG CCTTGCTCAG CAGATCTCCG ACGTGGTGAG GCAGCCGGCG 
TTCATTGCAG GCATTGGGGC AGCCTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGCTT 
TACAGACACC GCAAGAAGAG AAACGGACTC ACCAGCACGT ATGCGGGTAT CCGGAAAGTC 
CCGTCCTTTA CCTTCACGCC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT 
GGAGGGAGGC CGGGACTCCT CAACATTAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC 
ACGTGGCCCA ACACAGGCAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACGGCCGGC 
AATGGCAACA GCGACAGCAA CCTGACAACC TACAGTCGCC CAGCTGATTG TATAGCCAAT 
TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT 
GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG 
AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTATGCCAC CACTCAACTC 
ATCCAGGCGA ACCTCAGCAA CAATATGAAT AATGGCGCCG GGGACTCCAG CGAGAAGCAC 
TGGAAGCCTC CGGGACAGCA GAAACCGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG 
CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACGA TCCCTCCAAC CATCCCATAC 
AACCAGTCCT ATGACCAGAA CACAGGAGGG TCTTATAACA GCTCAGATCG GGGCAGTAGT 
ACATCCGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT 
GGCATGAACT GGGCAGACTT GCTGCCCCCT CCTCCGGCGC ATCCTCCCCC ACATAGCAAC 
AGTGAAGAAT ACAACATGTC CGTAGATGAA AGCTATGATC AAGAAATGCC CTGTCCAGTG 
CCACCTGCGC GGATGTATTT GCAACAGGAT GAATTAGAGG AAGAGGAAGA TGAAAGAGGC 
CCCACTCCCC CTGTCCGGGG GGCAGCTTCT TCTCCAGCTG CTGTGTCCTA CAGCCACCAA 
TCCACGGCCA CTCTCACCCC TTCTCCCCAG GAAGAGCTCC AGCCCATGTT ACAGGATTGT 
CCGGAGGACC TCGGGCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC CGTGAGTCCT 
CCTCCACCGC CACGGCCAAT TTCCCCACCT CATACTTACG GCTACATTTC AGGACCCCTT 
GTCTCAGATA TGGATACCGA TGCCCCAGAA GAGGAGGAGG ACGAAGCTGA CATGGAAGTT 
GCCAAAATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTGG AGCAGACCCC AGCATCCAGT 
GTTGGGGACC TTGAGAGCTC CGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCG 
GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCCGATGG CTCCTTTTTC 
ACTGACGCTG ATTTTGCCCA GGCAGTTGCT GCAGCTGCGG AGTATGCGGG CCTGAAAGTG 
GCTCGCCGCC AAATGCAAGA TGCTGCTGGC CGCCGCCACT TCCATGCCTC TCAGTGCCCA 
AGGCCCACGA GTCCTGTGTC CACAGACAGC AACATGAGTG CTGTTGTGAT CCAGAAAGCC 
AGACCCGCCA AGAAGCAGAA ACACCAGCCA GGACATCTGC GCAGGGAAGC CTACGCAGAT 
GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCACTGT CCAGTCCAAG 
GCACAGCTGG AGGTACGGCC TGTCATGGTG CCAAAACTCG CGTCTATAGA AGCAAGGACA 
GATAGATCGT CAGACAGAAA AGGAGGCAGT TACAAGGGGA GAGAAGCTCT GGATGGAAGA 
CAAGTCACTG ACCTGCGAAC AAATCCAAGT GACCCCAGAG AGGCACAGGA ACAGCCAAAT 
GACGGGAAAG GCCGAGGAAC AAAGGCAGCC AAGCGAGACC TTCCACCAGC AAAGACGCAC 
CTCGGCCAAG AGGATATTCT TCCGTACTGT AGACCTACTT TCCCAACGTC AAACAACCCT 
AGAGACCCCA GTTCCTCCAG CTCTATGTCA TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG 
CAAGCAAATG TGGGCCGAAG GAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA 
GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523024.1
CDS	471..5372
Translation	MAVLLLMLLFSMAEITSPVGVSGRMRAGSRWRGEKNRSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522961.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522961.3

ORF Insert Sequence:

ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT 
CGTCAGGAAG ATTTTCCACC CCGCATTGTT GAACACCCTT CAGACCTGAT CGTCTCCAAA 
GGAGAACCTG CCACTCTGAA CTGTAAAGCC GAAGGCCGCC CCACCCCGAC GATAGAATGG 
TACAAAGGTG GGGAAAGAGT GGAGACGGAC AAAGATGACC CGCGCTCACA CCGGATGCTG 
CTGCCAAGCG GGTCTTTATT TTTCTTACGC ATAGTCCATG GACGGAAGAG TAGGCCCGAC 
GAAGGGGTCT ACATCTGTGT GGCTAGGAAT TACCTCGGAG AGGCTGTGAG CCACAACGCA 
TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTCATGGTG 
GCAGTAGGCG AACCTGCAGT CATGGAATGC CAGCCTCCAC GGGGCCATCC AGAGCCCACC 
ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ATGAAAGAAT CACTATACGA 
GGAGGAAAAC TCATGATCAC TTATACGCGT AAAAGCGATG CTGGCAAGTA TGTTTGCGTT 
GGCACCAACA TGGTGGGGGA ACGGGAGAGT GAAGTCGCAG AGCTAACCGT TTTAGAGAGA 
CCATCGTTTG TGAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCAGAATTT 
AAATGTGAGG CCCGAGGAGA CCCTGTGCCT ACAGTTCGAT GGAGGAAAGA TGACGGAGAG 
CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACT 
GCTGGCGACA TGGGATCATA CACTTGTGTG GCAGAAAATA TGGTCGGGAA AGCTGAAGCT 
TCCGCTACTT TGACAGTTCA AGTTGGGTCT GAGCCGCCAC ATTTCGTTGT AAAACCTCGG 
GACCAGGTGG TTGCCTTGGG AAGAACTGTG ACGTTTCAGT GCGAAGCAAC TGGAAATCCT 
CAACCAGCTA TCTTTTGGAG GAGAGAGGGG AGTCAGAATC TACTTTTCTC CTACCAGCCT 
CCACAGTCCT CTAGCCGATT TTCAGTTTCG CAGACAGGAG ACCTCACCAT TACGAACGTC 
CAGCGGTCTG ATGTCGGCTA CTACATCTGT CAGACTTTAA ATGTTGCTGG AAGTATCATC 
ACAAAGGCCT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGG 
CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGCACTT TAATCCTCAG CTGTGTGGCC 
ACAGGCAGTC CAGCACCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA 
GATTCTCGGA TCAAACAGCT GGAGAGTGGC GTGCTGCAGA TCCGCTACGC TAAGCTGGGT 
GACACGGGTC GGTACACCTG CACCGCATCG ACCCCCAGCG GTGAGGCCAC GTGGAGCGCT 
TACATCGAAG TCCAGGAATT TGGGGTTCCA GTTCAACCCC CAAGACCCAC CGACCCCAAC 
TTAATCCCCA GCGCCCCCTC AAAGCCCGAA GTTACAGATG TCAGCAAAAA CACGGTCACT 
TTATCGTGGC AGCCAAACTT GAACTCAGGA GCAACCCCAA CATCTTATAT TATAGAAGCC 
TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACGGCGGCAG AAAATGTGAA AACAGAAACA 
TTTGCCATCA AAGGACTCAA ACCTAACGCC ATTTACCTTT TCCTGGTGAG GGCAGCAAAT 
GCCTATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACGCA AGATGTCCCA 
CCAACCAGCC AAGGGGTGGA CCACAAGCAG GTCCAAAGAG AACTGGGGAA TGTTGTCTTA 
CATCTGCACA ACCCGACCAT CCTTTCTTCC TCCTCAGTCG AAGTGCACTG GACGGTGGAC 
CAACAATCTC AATATATTCA AGGCTATAAA ATTCTCTACC GCCCATCTGG AGCCAGCCAT 
GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACGCCAA CGAAAAACAG CGTGGTGATC 
CCCGATCTCA GAAAAGGCGT CAACTACGAG ATTAAGGCTC GCCCATTTTT TAATGAGTTT 
CAAGGAGCAG ACAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT 
CCCCGAAGTG TAACTGTATC CAAGAATGAT GGAAATGGGA CTGCAATCCT GGTCACTTGG 
CAACCGCCTC CTGAAGACAC ACAAAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC 
GGTAATGAAA CGAAGTATCA CATAAACAAG ACAGTCGATG GCTCGACCTT CTCGGTGGTC 
ATCCCCTCTC TGGTTCCTGG GATCCGATAC AGTGTGGAGG TGGCAGCGAG CACTGGGGCG 
GGTCCCGGGG TGAAGAGCGA ACCTCAGTTC ATCCAGTTAG ATTCTCACGG AAACCCCGTG 
TCTCCCGAGG ACCAAGTCAG CCTTGCTCAG CAGATCTCCG ACGTGGTGAG GCAGCCGGCG 
TTCATTGCAG GCATTGGGGC AGCCTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGCTT 
TACAGACACC GCAAGAAGAG AAACGGACTC ACCAGCACGT ATGCGGGTAT CCGGAAAGTC 
CCGTCCTTTA CCTTCACGCC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT 
GGAGGGAGGC CGGGACTCCT CAACATTAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC 
ACGTGGCCCA ACACAGGCAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACGGCCGGC 
AATGGCAACA GCGACAGCAA CCTGACAACC TACAGTCGCC CAGCTGATTG TATAGCCAAT 
TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT 
GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG 
AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTATGCCAC CACTCAACTC 
ATCCAGGCGA ACCTCAGCAA CAATATGAAT AATGGCGCCG GGGACTCCAG CGAGAAGCAC 
TGGAAGCCTC CGGGACAGCA GAAACCGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG 
CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACGA TCCCTCCAAC CATCCCATAC 
AACCAGTCCT ATGACCAGAA CACAGGAGGG TCTTATAACA GCTCAGATCG GGGCAGTAGT 
ACATCCGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT 
GGCATGAACT GGGCAGACTT GCTGCCCCCT CCTCCGGCGC ATCCTCCCCC ACATAGCAAC 
AGTGAAGAAT ACAACATGTC CGTAGATGAA AGCTATGATC AAGAAATGCC CTGTCCAGTG 
CCACCTGCGC GGATGTATTT GCAACAGGAT GAATTAGAGG AAGAGGAAGA TGAAAGAGGC 
CCCACTCCCC CTGTCCGGGG GGCAGCTTCT TCTCCAGCTG CTGTGTCCTA CAGCCACCAA 
TCCACGGCCA CTCTCACCCC TTCTCCCCAG GAAGAGCTCC AGCCCATGTT ACAGGATTGT 
CCGGAGGACC TCGGGCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC CGTGAGTCCT 
CCTCCACCGC CACGGCCAAT TTCCCCACCT CATACTTACG GCTACATTTC AGGACCCCTT 
GTCTCAGATA TGGATACCGA TGCCCCAGAA GAGGAGGAGG ACGAAGCTGA CATGGAAGTT 
GCCAAAATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTGG AGCAGACCCC AGCATCCAGT 
GTTGGGGACC TTGAGAGCTC CGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCG 
GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCCGATGG CTCCTTTTTC 
ACTGACGCTG ATTTTGCCCA GGCAGTTGCT GCAGCTGCGG AGTATGCGGG CCTGAAAGTG 
GCTCGCCGCC AAATGCAAGA TGCTGCTGGC CGCCGCCACT TCCATGCCTC TCAGTGCCCA 
AGGCCCACGA GTCCTGTGTC CACAGACAGC AACATGAGTG CTGTTGTGAT CCAGAAAGCC 
AGACCCGCCA AGAAGCAGAA ACACCAGCCA GGACATCTGC GCAGGGAAGC CTACGCAGAT 
GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCACTGT CCAGTCCAAG 
GCACAGCTGG AGGTACGGCC TGTCATGGTG CCAAAACTCG CGTCTATAGA AGCAAGGACA 
GATAGATCGT CAGACAGAAA AGGAGGCAGT TACAAGGGGA GAGAAGCTCT GGATGGAAGA 
CAAGTCACTG ACCTGCGAAC AAATCCAAGT GACCCCAGAG AGGCACAGGA ACAGCCAAAT 
GACGGGAAAG GCCGAGGAAC AAAGGCAGCC AAGCGAGACC TTCCACCAGC AAAGACGCAC 
CTCGGCCAAG AGGATATTCT TCCGTACTGT AGACCTACTT TCCCAACGTC AAACAACCCT 
AGAGACCCCA GTTCCTCCAG CTCTATGTCA TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG 
CAAGCAAATG TGGGCCGAAG GAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA 
GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522962.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523025.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu52702
Clone ID Related Accession (Same CDS sequence)	XM_006522962.4 , XM_006522962.3
Accession Version	XM_006522962.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522962.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG 
CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC 
CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC 
AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC 
AACCTGACAA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC 
CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG 
AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC 
CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA 
TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC 
ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT 
GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA 
CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC 
ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC 
ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG 
GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT 
CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC 
TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC 
AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT 
GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA 
GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT 
GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT 
CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG 
CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA 
CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT 
CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA 
CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT 
AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA 
GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC 
GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC 
GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA 
GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA 
GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA 
GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523025.1
CDS	98..4996
Translation	MVLRPARGSVCCRKGKEDVCCVLSLCVFLSHVDLLVSGHAVIEMATTVTSVTPSAALVLFPTDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522962.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522962.4

ORF Insert Sequence:

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG 
CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC 
CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC 
AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC 
AACCTGACAA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC 
CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG 
AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC 
CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA 
TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC 
ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT 
GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA 
CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC 
ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC 
ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG 
GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT 
CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC 
TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC 
AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT 
GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA 
GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT 
GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT 
CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG 
CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA 
CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT 
CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA 
CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT 
AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA 
GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC 
GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC 
GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA 
GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA 
GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA 
GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522963.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523026.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA.

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CloneID	OMu52703
Clone ID Related Accession (Same CDS sequence)	XM_006522963.3 , XM_006522963.4
Accession Version	XM_006522963.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522963.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG GCTCCCGTCT TCGTCAGGAA 
GATTTTCCAC CCCGCATTGT TGAACACCCT TCAGACCTGA TCGTCTCCAA AGGAGAACCT 
GCCACTCTGA ACTGTAAAGC CGAAGGCCGC CCCACCCCGA CGATAGAATG GTACAAAGGT 
GGGGAAAGAG TGGAGACGGA CAAAGATGAC CCGCGCTCAC ACCGGATGCT GCTGCCAAGC 
GGGTCTTTAT TTTTCTTACG CATAGTCCAT GGACGGAAGA GTAGGCCCGA CGAAGGGGTC 
TACATCTGTG TGGCTAGGAA TTACCTCGGA GAGGCTGTGA GCCACAACGC ATCGCTGGAG 
GTAGCTATAC TACGGGATGA CTTCAGACAA AACCCTTCAG ATGTCATGGT GGCAGTAGGC 
GAACCTGCAG TCATGGAATG CCAGCCTCCA CGGGGCCATC CAGAGCCCAC CATCTCCTGG 
AAAAAGGATG GCTCACCCCT GGATGATAAA GATGAAAGAA TCACTATACG AGGAGGAAAA 
CTCATGATCA CTTATACGCG TAAAAGCGAT GCTGGCAAGT ATGTTTGCGT TGGCACCAAC 
ATGGTGGGGG AACGGGAGAG TGAAGTCGCA GAGCTAACCG TTTTAGAGAG ACCATCGTTT 
GTGAAGAGAC CCAGTAATTT GGCAGTGACT GTGGATGACA GTGCAGAATT TAAATGTGAG 
GCCCGAGGAG ACCCTGTGCC TACAGTTCGA TGGAGGAAAG ATGACGGAGA GCTGCCCAAA 
TCCAGATATG AAATCCGAGA TGATCATACT TTGAAAATTC GGAAGGTGAC TGCTGGCGAC 
ATGGGATCAT ACACTTGTGT GGCAGAAAAT ATGGTCGGGA AAGCTGAAGC TTCCGCTACT 
TTGACAGTTC AAGTTGGGTC TGAGCCGCCA CATTTCGTTG TAAAACCTCG GGACCAGGTG 
GTTGCCTTGG GAAGAACTGT GACGTTTCAG TGCGAAGCAA CTGGAAATCC TCAACCAGCT 
ATCTTTTGGA GGAGAGAGGG GAGTCAGAAT CTACTTTTCT CCTACCAGCC TCCACAGTCC 
TCTAGCCGAT TTTCAGTTTC GCAGACAGGA GACCTCACCA TTACGAACGT CCAGCGGTCT 
GATGTCGGCT ACTACATCTG TCAGACTTTA AATGTTGCTG GAAGTATCAT CACAAAGGCC 
TATTTGGAAG TTACTGATGT GATTGCAGAC CGGCCTCCCC CAGTCATTCG GCAAGGTCCT 
GTGAATCAGA CTGTAGCAGT GGATGGCACT TTAATCCTCA GCTGTGTGGC CACAGGCAGT 
CCAGCACCCA CAATTCTGTG GAGGAAGGAT GGAGTCCTTG TTTCAACCCA AGATTCTCGG 
ATCAAACAGC TGGAGAGTGG CGTGCTGCAG ATCCGCTACG CTAAGCTGGG TGACACGGGT 
CGGTACACCT GCACCGCATC GACCCCCAGC GGTGAGGCCA CGTGGAGCGC TTACATCGAA 
GTCCAGGAAT TTGGGGTTCC AGTTCAACCC CCAAGACCCA CCGACCCCAA CTTAATCCCC 
AGCGCCCCCT CAAAGCCCGA AGTTACAGAT GTCAGCAAAA ACACGGTCAC TTTATCGTGG 
CAGCCAAACT TGAACTCAGG AGCAACCCCA ACATCTTATA TTATAGAAGC CTTCAGCCAT 
GCATCTGGTA GCAGCTGGCA GACGGCGGCA GAAAATGTGA AAACAGAAAC ATTTGCCATC 
AAAGGACTCA AACCTAACGC CATTTACCTT TTCCTGGTGA GGGCAGCAAA TGCCTATGGA 
ATTAGTGATC CAAGCCAAAT ATCAGATCCA GTGAAAACGC AAGATGTCCC ACCAACCAGC 
CAAGGGGTGG ACCACAAGCA GGTCCAAAGA GAACTGGGGA ATGTTGTCTT ACATCTGCAC 
AACCCGACCA TCCTTTCTTC CTCCTCAGTC GAAGTGCACT GGACGGTGGA CCAACAATCT 
CAATATATTC AAGGCTATAA AATTCTCTAC CGCCCATCTG GAGCCAGCCA TGGTGAATCG 
GAGTGGTTAG TTTTTGAAGT GAGGACGCCA ACGAAAAACA GCGTGGTGAT CCCCGATCTC 
AGAAAAGGCG TCAACTACGA GATTAAGGCT CGCCCATTTT TTAATGAGTT TCAAGGAGCA 
GACAGTGAAA TCAAATTTGC CAAAACCTTA GAAGAAGCAC CAAGTGCCCC TCCCCGAAGT 
GTAACTGTAT CCAAGAATGA TGGAAATGGG ACTGCAATCC TGGTCACTTG GCAACCGCCT 
CCTGAAGACA CACAAAATGG AATGGTCCAA GAATATAAGG TTTGGTGTCT CGGTAATGAA 
ACGAAGTATC ACATAAACAA GACAGTCGAT GGCTCGACCT TCTCGGTGGT CATCCCCTCT 
CTGGTTCCTG GGATCCGATA CAGTGTGGAG GTGGCAGCGA GCACTGGGGC GGGTCCCGGG 
GTGAAGAGCG AACCTCAGTT CATCCAGTTA GATTCTCACG GAAACCCCGT GTCTCCCGAG 
GACCAAGTCA GCCTTGCTCA GCAGATCTCC GACGTGGTGA GGCAGCCGGC GTTCATTGCA 
GGCATTGGGG CAGCCTGTTG GATTATCCTC ATGGTCTTCA GCATCTGGCT TTACAGACAC 
CGCAAGAAGA GAAACGGACT CACCAGCACG TATGCGGGTA TCCGGAAAGT CCCGTCCTTT 
ACCTTCACGC CAACAGTAAC TTATCAAAGA GGAGGCGAAG CCGTCAGCAG TGGAGGGAGG 
CCGGGACTCC TCAACATTAG TGAACCTGCC ACGCAGCCAT GGCTGGCAGA CACGTGGCCC 
AACACAGGCA ACAACCACAA TGACTGCTCC ATCAACTGCT GCACGGCCGG CAATGGCAAC 
AGCGACAGCA ACCTGACAAC CTACAGTCGC CCAGCTGATT GTATAGCCAA TTATAACAAC 
CAACTGGATA ACAAACAAAC AAATCTGATG CTCCCTGAGT CAACTGTTTA TGGTGATGTG 
GACCTTAGTA ACAAAATCAA TGAGATGAAA ACCTTCAATA GCCCAAATCT GAAGGACGGG 
CGTTTTGTCA ATCCATCAGG GCAGCCCACT CCCTATGCCA CCACTCAACT CATCCAGGCG 
AACCTCAGCA ACAATATGAA TAATGGCGCC GGGGACTCCA GCGAGAAGCA CTGGAAGCCT 
CCGGGACAGC AGAAACCGGA AGTGGCACCA ATTCAGTATA ACATCATGGA GCAAAACAAG 
CTGAACAAAG ATTATCGGGC AAATGACACG ATCCCTCCAA CCATCCCATA CAACCAGTCC 
TATGACCAGA ACACAGGAGG GTCTTATAAC AGCTCAGATC GGGGCAGTAG TACATCCGGG 
AGTCAAGGCC ACAAGAAAGG GGCACGGACA CCAAAGGCAC CCAAGCAGGG TGGCATGAAC 
TGGGCAGACT TGCTGCCCCC TCCTCCGGCG CATCCTCCCC CACATAGCAA CAGTGAAGAA 
TACAACATGT CCGTAGATGA AAGCTATGAT CAAGAAATGC CCTGTCCAGT GCCACCTGCG 
CGGATGTATT TGCAACAGGA TGAATTAGAG GAAGAGGAAG ATGAAAGAGG CCCCACTCCC 
CCTGTCCGGG GGGCAGCTTC TTCTCCAGCT GCTGTGTCCT ACAGCCACCA ATCCACGGCC 
ACTCTCACCC CTTCTCCCCA GGAAGAGCTC CAGCCCATGT TACAGGATTG TCCGGAGGAC 
CTCGGGCACA TGCCCCACCC ACCAGACAGG AGGCGGCAGC CCGTGAGTCC TCCTCCACCG 
CCACGGCCAA TTTCCCCACC TCATACTTAC GGCTACATTT CAGGACCCCT TGTCTCAGAT 
ATGGATACCG ATGCCCCAGA AGAGGAGGAG GACGAAGCTG ACATGGAAGT TGCCAAAATG 
CAGACCCGAA GGCTGTTGTT ACGTGGGCTG GAGCAGACCC CAGCATCCAG TGTTGGGGAC 
CTTGAGAGCT CCGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC GGAAGAGGAC 
AACATTTCCA GTGGGCGCTC CAGTGTCAGT TCATCCGATG GCTCCTTTTT CACTGACGCT 
GATTTTGCCC AGGCAGTTGC TGCAGCTGCG GAGTATGCGG GCCTGAAAGT GGCTCGCCGC 
CAAATGCAAG ATGCTGCTGG CCGCCGCCAC TTCCATGCCT CTCAGTGCCC AAGGCCCACG 
AGTCCTGTGT CCACAGACAG CAACATGAGT GCTGTTGTGA TCCAGAAAGC CAGACCCGCC 
AAGAAGCAGA AACACCAGCC AGGACATCTG CGCAGGGAAG CCTACGCAGA TGATCTTCCA 
CCCCCTCCAG TGCCACCACC TGCTATAAAA TCGCCCACTG TCCAGTCCAA GGCACAGCTG 
GAGGTACGGC CTGTCATGGT GCCAAAACTC GCGTCTATAG AAGCAAGGAC AGATAGATCG 
TCAGACAGAA AAGGAGGCAG TTACAAGGGG AGAGAAGCTC TGGATGGAAG ACAAGTCACT 
GACCTGCGAA CAAATCCAAG TGACCCCAGA GAGGCACAGG AACAGCCAAA TGACGGGAAA 
GGCCGAGGAA CAAAGGCAGC CAAGCGAGAC CTTCCACCAG CAAAGACGCA CCTCGGCCAA 
GAGGATATTC TTCCGTACTG TAGACCTACT TTCCCAACGT CAAACAACCC TAGAGACCCC 
AGTTCCTCCA GCTCTATGTC ATCAAGAGGA TCAGGGAGCC GACAAAGAGA GCAAGCAAAT 
GTGGGCCGAA GGAATATGGC AGAAATGCAA GTACTTGGGG GATTTGAAAG AGGAGATGAG 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523026.1
CDS	426..5318
Translation	MGGIKMKWKHLPLLVISLLTLSRKQLLLAQLIPGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522963.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522963.4

ORF Insert Sequence:

ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG GCTCCCGTCT TCGTCAGGAA 
GATTTTCCAC CCCGCATTGT TGAACACCCT TCAGACCTGA TCGTCTCCAA AGGAGAACCT 
GCCACTCTGA ACTGTAAAGC CGAAGGCCGC CCCACCCCGA CGATAGAATG GTACAAAGGT 
GGGGAAAGAG TGGAGACGGA CAAAGATGAC CCGCGCTCAC ACCGGATGCT GCTGCCAAGC 
GGGTCTTTAT TTTTCTTACG CATAGTCCAT GGACGGAAGA GTAGGCCCGA CGAAGGGGTC 
TACATCTGTG TGGCTAGGAA TTACCTCGGA GAGGCTGTGA GCCACAACGC ATCGCTGGAG 
GTAGCTATAC TACGGGATGA CTTCAGACAA AACCCTTCAG ATGTCATGGT GGCAGTAGGC 
GAACCTGCAG TCATGGAATG CCAGCCTCCA CGGGGCCATC CAGAGCCCAC CATCTCCTGG 
AAAAAGGATG GCTCACCCCT GGATGATAAA GATGAAAGAA TCACTATACG AGGAGGAAAA 
CTCATGATCA CTTATACGCG TAAAAGCGAT GCTGGCAAGT ATGTTTGCGT TGGCACCAAC 
ATGGTGGGGG AACGGGAGAG TGAAGTCGCA GAGCTAACCG TTTTAGAGAG ACCATCGTTT 
GTGAAGAGAC CCAGTAATTT GGCAGTGACT GTGGATGACA GTGCAGAATT TAAATGTGAG 
GCCCGAGGAG ACCCTGTGCC TACAGTTCGA TGGAGGAAAG ATGACGGAGA GCTGCCCAAA 
TCCAGATATG AAATCCGAGA TGATCATACT TTGAAAATTC GGAAGGTGAC TGCTGGCGAC 
ATGGGATCAT ACACTTGTGT GGCAGAAAAT ATGGTCGGGA AAGCTGAAGC TTCCGCTACT 
TTGACAGTTC AAGTTGGGTC TGAGCCGCCA CATTTCGTTG TAAAACCTCG GGACCAGGTG 
GTTGCCTTGG GAAGAACTGT GACGTTTCAG TGCGAAGCAA CTGGAAATCC TCAACCAGCT 
ATCTTTTGGA GGAGAGAGGG GAGTCAGAAT CTACTTTTCT CCTACCAGCC TCCACAGTCC 
TCTAGCCGAT TTTCAGTTTC GCAGACAGGA GACCTCACCA TTACGAACGT CCAGCGGTCT 
GATGTCGGCT ACTACATCTG TCAGACTTTA AATGTTGCTG GAAGTATCAT CACAAAGGCC 
TATTTGGAAG TTACTGATGT GATTGCAGAC CGGCCTCCCC CAGTCATTCG GCAAGGTCCT 
GTGAATCAGA CTGTAGCAGT GGATGGCACT TTAATCCTCA GCTGTGTGGC CACAGGCAGT 
CCAGCACCCA CAATTCTGTG GAGGAAGGAT GGAGTCCTTG TTTCAACCCA AGATTCTCGG 
ATCAAACAGC TGGAGAGTGG CGTGCTGCAG ATCCGCTACG CTAAGCTGGG TGACACGGGT 
CGGTACACCT GCACCGCATC GACCCCCAGC GGTGAGGCCA CGTGGAGCGC TTACATCGAA 
GTCCAGGAAT TTGGGGTTCC AGTTCAACCC CCAAGACCCA CCGACCCCAA CTTAATCCCC 
AGCGCCCCCT CAAAGCCCGA AGTTACAGAT GTCAGCAAAA ACACGGTCAC TTTATCGTGG 
CAGCCAAACT TGAACTCAGG AGCAACCCCA ACATCTTATA TTATAGAAGC CTTCAGCCAT 
GCATCTGGTA GCAGCTGGCA GACGGCGGCA GAAAATGTGA AAACAGAAAC ATTTGCCATC 
AAAGGACTCA AACCTAACGC CATTTACCTT TTCCTGGTGA GGGCAGCAAA TGCCTATGGA 
ATTAGTGATC CAAGCCAAAT ATCAGATCCA GTGAAAACGC AAGATGTCCC ACCAACCAGC 
CAAGGGGTGG ACCACAAGCA GGTCCAAAGA GAACTGGGGA ATGTTGTCTT ACATCTGCAC 
AACCCGACCA TCCTTTCTTC CTCCTCAGTC GAAGTGCACT GGACGGTGGA CCAACAATCT 
CAATATATTC AAGGCTATAA AATTCTCTAC CGCCCATCTG GAGCCAGCCA TGGTGAATCG 
GAGTGGTTAG TTTTTGAAGT GAGGACGCCA ACGAAAAACA GCGTGGTGAT CCCCGATCTC 
AGAAAAGGCG TCAACTACGA GATTAAGGCT CGCCCATTTT TTAATGAGTT TCAAGGAGCA 
GACAGTGAAA TCAAATTTGC CAAAACCTTA GAAGAAGCAC CAAGTGCCCC TCCCCGAAGT 
GTAACTGTAT CCAAGAATGA TGGAAATGGG ACTGCAATCC TGGTCACTTG GCAACCGCCT 
CCTGAAGACA CACAAAATGG AATGGTCCAA GAATATAAGG TTTGGTGTCT CGGTAATGAA 
ACGAAGTATC ACATAAACAA GACAGTCGAT GGCTCGACCT TCTCGGTGGT CATCCCCTCT 
CTGGTTCCTG GGATCCGATA CAGTGTGGAG GTGGCAGCGA GCACTGGGGC GGGTCCCGGG 
GTGAAGAGCG AACCTCAGTT CATCCAGTTA GATTCTCACG GAAACCCCGT GTCTCCCGAG 
GACCAAGTCA GCCTTGCTCA GCAGATCTCC GACGTGGTGA GGCAGCCGGC GTTCATTGCA 
GGCATTGGGG CAGCCTGTTG GATTATCCTC ATGGTCTTCA GCATCTGGCT TTACAGACAC 
CGCAAGAAGA GAAACGGACT CACCAGCACG TATGCGGGTA TCCGGAAAGT CCCGTCCTTT 
ACCTTCACGC CAACAGTAAC TTATCAAAGA GGAGGCGAAG CCGTCAGCAG TGGAGGGAGG 
CCGGGACTCC TCAACATTAG TGAACCTGCC ACGCAGCCAT GGCTGGCAGA CACGTGGCCC 
AACACAGGCA ACAACCACAA TGACTGCTCC ATCAACTGCT GCACGGCCGG CAATGGCAAC 
AGCGACAGCA ACCTGACAAC CTACAGTCGC CCAGCTGATT GTATAGCCAA TTATAACAAC 
CAACTGGATA ACAAACAAAC AAATCTGATG CTCCCTGAGT CAACTGTTTA TGGTGATGTG 
GACCTTAGTA ACAAAATCAA TGAGATGAAA ACCTTCAATA GCCCAAATCT GAAGGACGGG 
CGTTTTGTCA ATCCATCAGG GCAGCCCACT CCCTATGCCA CCACTCAACT CATCCAGGCG 
AACCTCAGCA ACAATATGAA TAATGGCGCC GGGGACTCCA GCGAGAAGCA CTGGAAGCCT 
CCGGGACAGC AGAAACCGGA AGTGGCACCA ATTCAGTATA ACATCATGGA GCAAAACAAG 
CTGAACAAAG ATTATCGGGC AAATGACACG ATCCCTCCAA CCATCCCATA CAACCAGTCC 
TATGACCAGA ACACAGGAGG GTCTTATAAC AGCTCAGATC GGGGCAGTAG TACATCCGGG 
AGTCAAGGCC ACAAGAAAGG GGCACGGACA CCAAAGGCAC CCAAGCAGGG TGGCATGAAC 
TGGGCAGACT TGCTGCCCCC TCCTCCGGCG CATCCTCCCC CACATAGCAA CAGTGAAGAA 
TACAACATGT CCGTAGATGA AAGCTATGAT CAAGAAATGC CCTGTCCAGT GCCACCTGCG 
CGGATGTATT TGCAACAGGA TGAATTAGAG GAAGAGGAAG ATGAAAGAGG CCCCACTCCC 
CCTGTCCGGG GGGCAGCTTC TTCTCCAGCT GCTGTGTCCT ACAGCCACCA ATCCACGGCC 
ACTCTCACCC CTTCTCCCCA GGAAGAGCTC CAGCCCATGT TACAGGATTG TCCGGAGGAC 
CTCGGGCACA TGCCCCACCC ACCAGACAGG AGGCGGCAGC CCGTGAGTCC TCCTCCACCG 
CCACGGCCAA TTTCCCCACC TCATACTTAC GGCTACATTT CAGGACCCCT TGTCTCAGAT 
ATGGATACCG ATGCCCCAGA AGAGGAGGAG GACGAAGCTG ACATGGAAGT TGCCAAAATG 
CAGACCCGAA GGCTGTTGTT ACGTGGGCTG GAGCAGACCC CAGCATCCAG TGTTGGGGAC 
CTTGAGAGCT CCGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC GGAAGAGGAC 
AACATTTCCA GTGGGCGCTC CAGTGTCAGT TCATCCGATG GCTCCTTTTT CACTGACGCT 
GATTTTGCCC AGGCAGTTGC TGCAGCTGCG GAGTATGCGG GCCTGAAAGT GGCTCGCCGC 
CAAATGCAAG ATGCTGCTGG CCGCCGCCAC TTCCATGCCT CTCAGTGCCC AAGGCCCACG 
AGTCCTGTGT CCACAGACAG CAACATGAGT GCTGTTGTGA TCCAGAAAGC CAGACCCGCC 
AAGAAGCAGA AACACCAGCC AGGACATCTG CGCAGGGAAG CCTACGCAGA TGATCTTCCA 
CCCCCTCCAG TGCCACCACC TGCTATAAAA TCGCCCACTG TCCAGTCCAA GGCACAGCTG 
GAGGTACGGC CTGTCATGGT GCCAAAACTC GCGTCTATAG AAGCAAGGAC AGATAGATCG 
TCAGACAGAA AAGGAGGCAG TTACAAGGGG AGAGAAGCTC TGGATGGAAG ACAAGTCACT 
GACCTGCGAA CAAATCCAAG TGACCCCAGA GAGGCACAGG AACAGCCAAA TGACGGGAAA 
GGCCGAGGAA CAAAGGCAGC CAAGCGAGAC CTTCCACCAG CAAAGACGCA CCTCGGCCAA 
GAGGATATTC TTCCGTACTG TAGACCTACT TTCCCAACGT CAAACAACCC TAGAGACCCC 
AGTTCCTCCA GCTCTATGTC ATCAAGAGGA TCAGGGAGCC GACAAAGAGA GCAAGCAAAT 
GTGGGCCGAA GGAATATGGC AGAAATGCAA GTACTTGGGG GATTTGAAAG AGGAGATGAG 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522964.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523027.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X11, mRNA.

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CloneID	OMu52704
Clone ID Related Accession (Same CDS sequence)	XM_006522964.4 , XM_006522964.3
Accession Version	XM_006522964.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522964.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGGG 
CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT 
AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA 
GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA 
AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG 
TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG 
GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT 
CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA 
AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT 
GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT 
TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG 
GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA 
CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT 
CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA 
GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA 
CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG 
TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC 
AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT 
GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC 
CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC 
AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA 
GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT 
GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG 
CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT 
TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT 
GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC 
AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT 
AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA 
TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA 
GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523027.1
CDS	99..4970
Translation	MVLRPARGSVCCRKGKEDVCCVLSLCVFLSHVDLLVSGHAVIEMATTVTSVTPSAALVLFPTDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522964.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522964.4

ORF Insert Sequence:

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGGG 
CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT 
AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA 
GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA 
AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG 
TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG 
GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT 
CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA 
AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT 
GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT 
TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG 
GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA 
CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT 
CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA 
GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA 
CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG 
TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC 
AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT 
GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC 
CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC 
AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA 
GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT 
GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG 
CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT 
TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT 
GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC 
AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT 
AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA 
TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA 
GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522965.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523028.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X13, mRNA.

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CloneID	OMu52705
Clone ID Related Accession (Same CDS sequence)	XM_006522965.1 , XM_006522965.2
Accession Version	XM_006522965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC 
TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA 
GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG 
ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG 
CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC 
AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC 
ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG 
CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT 
ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT 
TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA 
GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG 
GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT 
GAAGCTTCCG CTACTTTGAC AGTTCAAGTT GGGTCTGAGC CGCCACATTT CGTTGTAAAA 
CCTCGGGACC AGGTGGTTGC CTTGGGAAGA ACTGTGACGT TTCAGTGCGA AGCAACTGGA 
AATCCTCAAC CAGCTATCTT TTGGAGGAGA GAGGGGAGTC AGAATCTACT TTTCTCCTAC 
CAGCCTCCAC AGTCCTCTAG CCGATTTTCA GTTTCGCAGA CAGGAGACCT CACCATTACG 
AACGTCCAGC GGTCTGATGT CGGCTACTAC ATCTGTCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AGGCCTATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC 
ATTCGGCAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GCACTTTAAT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGTGGCGTGC TGCAGATCCG CTACGCTAAG 
CTGGGTGACA CGGGTCGGTA CACCTGCACC GCATCGACCC CCAGCGGTGA GGCCACGTGG 
AGCGCTTACA TCGAAGTCCA GGAATTTGGG GTTCCAGTTC AACCCCCAAG ACCCACCGAC 
CCCAACTTAA TCCCCAGCGC CCCCTCAAAG CCCGAAGTTA CAGATGTCAG CAAAAACACG 
GTCACTTTAT CGTGGCAGCC AAACTTGAAC TCAGGAGCAA CCCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACGG CGGCAGAAAA TGTGAAAACA 
GAAACATTTG CCATCAAAGG ACTCAAACCT AACGCCATTT ACCTTTTCCT GGTGAGGGCA 
GCAAATGCCT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACGCAAGAT 
GTCCCACCAA CCAGCCAAGG GGTGGACCAC AAGCAGGTCC AAAGAGAACT GGGGAATGTT 
GTCTTACATC TGCACAACCC GACCATCCTT TCTTCCTCCT CAGTCGAAGT GCACTGGACG 
GTGGACCAAC AATCTCAATA TATTCAAGGC TATAAAATTC TCTACCGCCC ATCTGGAGCC 
AGCCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CGCCAACGAA AAACAGCGTG 
GTGATCCCCG ATCTCAGAAA AGGCGTCAAC TACGAGATTA AGGCTCGCCC ATTTTTTAAT 
GAGTTTCAAG GAGCAGACAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT 
GCCCCTCCCC GAAGTGTAAC TGTATCCAAG AATGATGGAA ATGGGACTGC AATCCTGGTC 
ACTTGGCAAC CGCCTCCTGA AGACACACAA AATGGAATGG TCCAAGAATA TAAGGTTTGG 
TGTCTCGGTA ATGAAACGAA GTATCACATA AACAAGACAG TCGATGGCTC GACCTTCTCG 
GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGATACAGTG TGGAGGTGGC AGCGAGCACT 
GGGGCGGGTC CCGGGGTGAA GAGCGAACCT CAGTTCATCC AGTTAGATTC TCACGGAAAC 
CCCGTGTCTC CCGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCCGACGT GGTGAGGCAG 
CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC TGTTGGATTA TCCTCATGGT CTTCAGCATC 
TGGCTTTACA GACACCGCAA GAAGAGAAAC GGACTCACCA GCACGTATGC GGGTATCCGG 
AAAGTCCCGT CCTTTACCTT CACGCCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC 
AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATTAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCCAACAC AGGCAACAAC CACAATGACT GCTCCATCAA CTGCTGCACG 
GCCGGCAATG GCAACAGCGA CAGCAACCTG ACAACCTACA GTCGCCCAGC TGATTGTATA 
GCCAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA TGCCACCACT 
CAACTCATCC AGGCGAACCT CAGCAACAAT ATGAATAATG GCGCCGGGGA CTCCAGCGAG 
AAGCACTGGA AGCCTCCGGG ACAGCAGAAA CCGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACGATCCC TCCAACCATC 
CCATACAACC AGTCCTATGA CCAGAACACA GGAGGGTCTT ATAACAGCTC AGATCGGGGC 
AGTAGTACAT CCGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG 
CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCTC CGGCGCATCC TCCCCCACAT 
AGCAACAGTG AAGAATACAA CATGTCCGTA GATGAAAGCT ATGATCAAGA AATGCCCTGT 
CCAGTGCCAC CTGCGCGGAT GTATTTGCAA CAGGATGAAT TAGAGGAAGA GGAAGATGAA 
AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA GCTTCTTCTC CAGCTGCTGT GTCCTACAGC 
CACCAATCCA CGGCCACTCT CACCCCTTCT CCCCAGGAAG AGCTCCAGCC CATGTTACAG 
GATTGTCCGG AGGACCTCGG GCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCCGTG 
AGTCCTCCTC CACCGCCACG GCCAATTTCC CCACCTCATA CTTACGGCTA CATTTCAGGA 
CCCCTTGTCT CAGATATGGA TACCGATGCC CCAGAAGAGG AGGAGGACGA AGCTGACATG 
GAAGTTGCCA AAATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTGGAGCA GACCCCAGCA 
TCCAGTGTTG GGGACCTTGA GAGCTCCGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA 
GCCTCGGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC CGATGGCTCC 
TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG 
AAAGTGGCTC GCCGCCAAAT GCAAGATGCT GCTGGCCGCC GCCACTTCCA TGCCTCTCAG 
TGCCCAAGGC CCACGAGTCC TGTGTCCACA GACAGCAACA TGAGTGCTGT TGTGATCCAG 
AAAGCCAGAC CCGCCAAGAA GCAGAAACAC CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC 
GCAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CACTGTCCAG 
TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC ATGGTGCCAA AACTCGCGTC TATAGAAGCA 
AGGACAGATA GATCGTCAGA CAGAAAAGGA GGCAGTTACA AGGGGAGAGA AGCTCTGGAT 
GGAAGACAAG TCACTGACCT GCGAACAAAT CCAAGTGACC CCAGAGAGGC ACAGGAACAG 
CCAAATGACG GGAAAGGCCG AGGAACAAAG GCAGCCAAGC GAGACCTTCC ACCAGCAAAG 
ACGCACCTCG GCCAAGAGGA TATTCTTCCG TACTGTAGAC CTACTTTCCC AACGTCAAAC 
AACCCTAGAG ACCCCAGTTC CTCCAGCTCT ATGTCATCAA GAGGATCAGG GAGCCGACAA 
AGAGAGCAAG CAAATGTGGG CCGAAGGAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT 
GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523028.1
CDS	1366..6213
Translation	MIAEPARFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522965.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522965.2

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC 
TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA 
GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG 
ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG 
CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC 
AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC 
ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG 
CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT 
ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT 
TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA 
GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG 
GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT 
GAAGCTTCCG CTACTTTGAC AGTTCAAGTT GGGTCTGAGC CGCCACATTT CGTTGTAAAA 
CCTCGGGACC AGGTGGTTGC CTTGGGAAGA ACTGTGACGT TTCAGTGCGA AGCAACTGGA 
AATCCTCAAC CAGCTATCTT TTGGAGGAGA GAGGGGAGTC AGAATCTACT TTTCTCCTAC 
CAGCCTCCAC AGTCCTCTAG CCGATTTTCA GTTTCGCAGA CAGGAGACCT CACCATTACG 
AACGTCCAGC GGTCTGATGT CGGCTACTAC ATCTGTCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AGGCCTATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC 
ATTCGGCAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GCACTTTAAT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGTGGCGTGC TGCAGATCCG CTACGCTAAG 
CTGGGTGACA CGGGTCGGTA CACCTGCACC GCATCGACCC CCAGCGGTGA GGCCACGTGG 
AGCGCTTACA TCGAAGTCCA GGAATTTGGG GTTCCAGTTC AACCCCCAAG ACCCACCGAC 
CCCAACTTAA TCCCCAGCGC CCCCTCAAAG CCCGAAGTTA CAGATGTCAG CAAAAACACG 
GTCACTTTAT CGTGGCAGCC AAACTTGAAC TCAGGAGCAA CCCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACGG CGGCAGAAAA TGTGAAAACA 
GAAACATTTG CCATCAAAGG ACTCAAACCT AACGCCATTT ACCTTTTCCT GGTGAGGGCA 
GCAAATGCCT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACGCAAGAT 
GTCCCACCAA CCAGCCAAGG GGTGGACCAC AAGCAGGTCC AAAGAGAACT GGGGAATGTT 
GTCTTACATC TGCACAACCC GACCATCCTT TCTTCCTCCT CAGTCGAAGT GCACTGGACG 
GTGGACCAAC AATCTCAATA TATTCAAGGC TATAAAATTC TCTACCGCCC ATCTGGAGCC 
AGCCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CGCCAACGAA AAACAGCGTG 
GTGATCCCCG ATCTCAGAAA AGGCGTCAAC TACGAGATTA AGGCTCGCCC ATTTTTTAAT 
GAGTTTCAAG GAGCAGACAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT 
GCCCCTCCCC GAAGTGTAAC TGTATCCAAG AATGATGGAA ATGGGACTGC AATCCTGGTC 
ACTTGGCAAC CGCCTCCTGA AGACACACAA AATGGAATGG TCCAAGAATA TAAGGTTTGG 
TGTCTCGGTA ATGAAACGAA GTATCACATA AACAAGACAG TCGATGGCTC GACCTTCTCG 
GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGATACAGTG TGGAGGTGGC AGCGAGCACT 
GGGGCGGGTC CCGGGGTGAA GAGCGAACCT CAGTTCATCC AGTTAGATTC TCACGGAAAC 
CCCGTGTCTC CCGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCCGACGT GGTGAGGCAG 
CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC TGTTGGATTA TCCTCATGGT CTTCAGCATC 
TGGCTTTACA GACACCGCAA GAAGAGAAAC GGACTCACCA GCACGTATGC GGGTATCCGG 
AAAGTCCCGT CCTTTACCTT CACGCCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC 
AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATTAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCCAACAC AGGCAACAAC CACAATGACT GCTCCATCAA CTGCTGCACG 
GCCGGCAATG GCAACAGCGA CAGCAACCTG ACAACCTACA GTCGCCCAGC TGATTGTATA 
GCCAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA TGCCACCACT 
CAACTCATCC AGGCGAACCT CAGCAACAAT ATGAATAATG GCGCCGGGGA CTCCAGCGAG 
AAGCACTGGA AGCCTCCGGG ACAGCAGAAA CCGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACGATCCC TCCAACCATC 
CCATACAACC AGTCCTATGA CCAGAACACA GGAGGGTCTT ATAACAGCTC AGATCGGGGC 
AGTAGTACAT CCGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG 
CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCTC CGGCGCATCC TCCCCCACAT 
AGCAACAGTG AAGAATACAA CATGTCCGTA GATGAAAGCT ATGATCAAGA AATGCCCTGT 
CCAGTGCCAC CTGCGCGGAT GTATTTGCAA CAGGATGAAT TAGAGGAAGA GGAAGATGAA 
AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA GCTTCTTCTC CAGCTGCTGT GTCCTACAGC 
CACCAATCCA CGGCCACTCT CACCCCTTCT CCCCAGGAAG AGCTCCAGCC CATGTTACAG 
GATTGTCCGG AGGACCTCGG GCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCCGTG 
AGTCCTCCTC CACCGCCACG GCCAATTTCC CCACCTCATA CTTACGGCTA CATTTCAGGA 
CCCCTTGTCT CAGATATGGA TACCGATGCC CCAGAAGAGG AGGAGGACGA AGCTGACATG 
GAAGTTGCCA AAATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTGGAGCA GACCCCAGCA 
TCCAGTGTTG GGGACCTTGA GAGCTCCGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA 
GCCTCGGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC CGATGGCTCC 
TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG 
AAAGTGGCTC GCCGCCAAAT GCAAGATGCT GCTGGCCGCC GCCACTTCCA TGCCTCTCAG 
TGCCCAAGGC CCACGAGTCC TGTGTCCACA GACAGCAACA TGAGTGCTGT TGTGATCCAG 
AAAGCCAGAC CCGCCAAGAA GCAGAAACAC CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC 
GCAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CACTGTCCAG 
TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC ATGGTGCCAA AACTCGCGTC TATAGAAGCA 
AGGACAGATA GATCGTCAGA CAGAAAAGGA GGCAGTTACA AGGGGAGAGA AGCTCTGGAT 
GGAAGACAAG TCACTGACCT GCGAACAAAT CCAAGTGACC CCAGAGAGGC ACAGGAACAG 
CCAAATGACG GGAAAGGCCG AGGAACAAAG GCAGCCAAGC GAGACCTTCC ACCAGCAAAG 
ACGCACCTCG GCCAAGAGGA TATTCTTCCG TACTGTAGAC CTACTTTCCC AACGTCAAAC 
AACCCTAGAG ACCCCAGTTC CTCCAGCTCT ATGTCATCAA GAGGATCAGG GAGCCGACAA 
AGAGAGCAAG CAAATGTGGG CCGAAGGAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT 
GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_017316893.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172382.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X1, mRNA.

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CloneID	OMu79277
Clone ID Related Accession (Same CDS sequence)	XM_017316893.1
Accession Version	XM_017316893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG 
CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC 
CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC 
AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC 
AACCTGACAA CCTACAGTCG CCCAGCTGAT TGTATAGCCA ATTATAACAA CCAACTGGAT 
AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT 
AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGACGG GCGTTTTGTC 
AATCCATCAG GGCAGCCCAC TCCCTATGCC ACCACTCAAC TCATCCAGGC GAACCTCAGC 
AACAATATGA ATAATGGCGC CGGGGACTCC AGCGAGAAGC ACTGGAAGCC TCCGGGACAG 
CAGAAACCGG AAGTGGCACC AATTCAGTAT AACATCATGG AGCAAAACAA GCTGAACAAA 
GATTATCGGG CAAATGACAC GATCCCTCCA ACCATCCCAT ACAACCAGTC CTATGACCAG 
AACACAGGAG GGTCTTATAA CAGCTCAGAT CGGGGCAGTA GTACATCCGG GAGTCAAGGC 
CACAAGAAAG GGGCACGGAC ACCAAAGGCA CCCAAGCAGG GTGGCATGAA CTGGGCAGAC 
TTGCTGCCCC CTCCTCCGGC GCATCCTCCC CCACATAGCA ACAGTGAAGA ATACAACATG 
TCCGTAGATG AAAGCTATGA TCAAGAAATG CCCTGTCCAG TGCCACCTGC GCGGATGTAT 
TTGCAACAGG ATGAATTAGA GGAAGAGGAA GATGAAAGAG GCCCCACTCC CCCTGTCCGG 
GGGGCAGCTT CTTCTCCAGC TGCTGTGTCC TACAGCCACC AATCCACGGC CACTCTCACC 
CCTTCTCCCC AGGAAGAGCT CCAGCCCATG TTACAGGATT GTCCGGAGGA CCTCGGGCAC 
ATGCCCCACC CACCAGACAG GAGGCGGCAG CCCGTGAGTC CTCCTCCACC GCCACGGCCA 
ATTTCCCCAC CTCATACTTA CGGCTACATT TCAGGACCCC TTGTCTCAGA TATGGATACC 
GATGCCCCAG AAGAGGAGGA GGACGAAGCT GACATGGAAG TTGCCAAAAT GCAGACCCGA 
AGGCTGTTGT TACGTGGGCT GGAGCAGACC CCAGCATCCA GTGTTGGGGA CCTTGAGAGC 
TCCGTCACTG GGTCCATGAT CAATGGCTGG GGCTCAGCCT CGGAAGAGGA CAACATTTCC 
AGTGGGCGCT CCAGTGTCAG TTCATCCGAT GGCTCCTTTT TCACTGACGC TGATTTTGCC 
CAGGCAGTTG CTGCAGCTGC GGAGTATGCG GGCCTGAAAG TGGCTCGCCG CCAAATGCAA 
GATGCTGCTG GCCGCCGCCA CTTCCATGCC TCTCAGTGCC CAAGGCCCAC GAGTCCTGTG 
TCCACAGACA GCAACATGAG TGCTGTTGTG ATCCAGAAAG CCAGACCCGC CAAGAAGCAG 
AAACACCAGC CAGGACATCT GCGCAGGGAA GCCTACGCAG ATGATCTTCC ACCCCCTCCA 
GTGCCACCAC CTGCTATAAA ATCGCCCACT GTCCAGTCCA AGGCACAGCT GGAGGTACGG 
CCTGTCATGG TGCCAAAACT CGCGTCTATA GAAGCAAGGA CAGATAGATC GTCAGACAGA 
AAAGGAGGCA GTTACAAGGG GAGAGAAGCT CTGGATGGAA GACAAGTCAC TGACCTGCGA 
ACAAATCCAA GTGACCCCAG AGAGGCACAG GAACAGCCAA ATGACGGGAA AGGCCGAGGA 
ACAAAGGCAG CCAAGCGAGA CCTTCCACCA GCAAAGACGC ACCTCGGCCA AGAGGATATT 
CTTCCGTACT GTAGACCTAC TTTCCCAACG TCAAACAACC CTAGAGACCC CAGTTCCTCC 
AGCTCTATGT CATCAAGAGG ATCAGGGAGC CGACAAAGAG AGCAAGCAAA TGTGGGCCGA 
AGGAATATGG CAGAAATGCA AGTACTTGGG GGATTTGAAA GAGGAGATGA GAATAATGAA 
GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172382.1
CDS	98..5161
Translation	MVLRPARGSVCCRKGKEDVCCVLSLCVFLSHVDLLVSGHAVIEMATTVTSVTPSAALVLFPTDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_017316893.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316893.1

ORF Insert Sequence:

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG 
CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC 
CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC 
AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC 
AACCTGACAA CCTACAGTCG CCCAGCTGAT TGTATAGCCA ATTATAACAA CCAACTGGAT 
AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT 
AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGACGG GCGTTTTGTC 
AATCCATCAG GGCAGCCCAC TCCCTATGCC ACCACTCAAC TCATCCAGGC GAACCTCAGC 
AACAATATGA ATAATGGCGC CGGGGACTCC AGCGAGAAGC ACTGGAAGCC TCCGGGACAG 
CAGAAACCGG AAGTGGCACC AATTCAGTAT AACATCATGG AGCAAAACAA GCTGAACAAA 
GATTATCGGG CAAATGACAC GATCCCTCCA ACCATCCCAT ACAACCAGTC CTATGACCAG 
AACACAGGAG GGTCTTATAA CAGCTCAGAT CGGGGCAGTA GTACATCCGG GAGTCAAGGC 
CACAAGAAAG GGGCACGGAC ACCAAAGGCA CCCAAGCAGG GTGGCATGAA CTGGGCAGAC 
TTGCTGCCCC CTCCTCCGGC GCATCCTCCC CCACATAGCA ACAGTGAAGA ATACAACATG 
TCCGTAGATG AAAGCTATGA TCAAGAAATG CCCTGTCCAG TGCCACCTGC GCGGATGTAT 
TTGCAACAGG ATGAATTAGA GGAAGAGGAA GATGAAAGAG GCCCCACTCC CCCTGTCCGG 
GGGGCAGCTT CTTCTCCAGC TGCTGTGTCC TACAGCCACC AATCCACGGC CACTCTCACC 
CCTTCTCCCC AGGAAGAGCT CCAGCCCATG TTACAGGATT GTCCGGAGGA CCTCGGGCAC 
ATGCCCCACC CACCAGACAG GAGGCGGCAG CCCGTGAGTC CTCCTCCACC GCCACGGCCA 
ATTTCCCCAC CTCATACTTA CGGCTACATT TCAGGACCCC TTGTCTCAGA TATGGATACC 
GATGCCCCAG AAGAGGAGGA GGACGAAGCT GACATGGAAG TTGCCAAAAT GCAGACCCGA 
AGGCTGTTGT TACGTGGGCT GGAGCAGACC CCAGCATCCA GTGTTGGGGA CCTTGAGAGC 
TCCGTCACTG GGTCCATGAT CAATGGCTGG GGCTCAGCCT CGGAAGAGGA CAACATTTCC 
AGTGGGCGCT CCAGTGTCAG TTCATCCGAT GGCTCCTTTT TCACTGACGC TGATTTTGCC 
CAGGCAGTTG CTGCAGCTGC GGAGTATGCG GGCCTGAAAG TGGCTCGCCG CCAAATGCAA 
GATGCTGCTG GCCGCCGCCA CTTCCATGCC TCTCAGTGCC CAAGGCCCAC GAGTCCTGTG 
TCCACAGACA GCAACATGAG TGCTGTTGTG ATCCAGAAAG CCAGACCCGC CAAGAAGCAG 
AAACACCAGC CAGGACATCT GCGCAGGGAA GCCTACGCAG ATGATCTTCC ACCCCCTCCA 
GTGCCACCAC CTGCTATAAA ATCGCCCACT GTCCAGTCCA AGGCACAGCT GGAGGTACGG 
CCTGTCATGG TGCCAAAACT CGCGTCTATA GAAGCAAGGA CAGATAGATC GTCAGACAGA 
AAAGGAGGCA GTTACAAGGG GAGAGAAGCT CTGGATGGAA GACAAGTCAC TGACCTGCGA 
ACAAATCCAA GTGACCCCAG AGAGGCACAG GAACAGCCAA ATGACGGGAA AGGCCGAGGA 
ACAAAGGCAG CCAAGCGAGA CCTTCCACCA GCAAAGACGC ACCTCGGCCA AGAGGATATT 
CTTCCGTACT GTAGACCTAC TTTCCCAACG TCAAACAACC CTAGAGACCC CAGTTCCTCC 
AGCTCTATGT CATCAAGAGG ATCAGGGAGC CGACAAAGAG AGCAAGCAAA TGTGGGCCGA 
AGGAATATGG CAGAAATGCA AGTACTTGGG GGATTTGAAA GAGGAGATGA GAATAATGAA 
GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_017316894.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172383.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu79278
Clone ID Related Accession (Same CDS sequence)	XM_017316894.1 , XM_017316894.2
Accession Version	XM_017316894.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCA CCCTGAGGAC 
CTAGAGAGGG GGAACGACAA CGGAACGCCA GCCCCCACCT CTGATAACGA TGACAATTCG 
CTGGGCTACA CAGGCTCCCG TCTTCGTCAG GAAGATTTTC CACCCCGCAT TGTTGAACAC 
CCTTCAGACC TGATCGTCTC CAAAGGAGAA CCTGCCACTC TGAACTGTAA AGCCGAAGGC 
CGCCCCACCC CGACGATAGA ATGGTACAAA GGTGGGGAAA GAGTGGAGAC GGACAAAGAT 
GACCCGCGCT CACACCGGAT GCTGCTGCCA AGCGGGTCTT TATTTTTCTT ACGCATAGTC 
CATGGACGGA AGAGTAGGCC CGACGAAGGG GTCTACATCT GTGTGGCTAG GAATTACCTC 
GGAGAGGCTG TGAGCCACAA CGCATCGCTG GAGGTAGCTA TACTACGGGA TGACTTCAGA 
CAAAACCCTT CAGATGTCAT GGTGGCAGTA GGCGAACCTG CAGTCATGGA ATGCCAGCCT 
CCACGGGGCC ATCCAGAGCC CACCATCTCC TGGAAAAAGG ATGGCTCACC CCTGGATGAT 
AAAGATGAAA GAATCACTAT ACGAGGAGGA AAACTCATGA TCACTTATAC GCGTAAAAGC 
GATGCTGGCA AGTATGTTTG CGTTGGCACC AACATGGTGG GGGAACGGGA GAGTGAAGTC 
GCAGAGCTAA CCGTTTTAGA GAGACCATCG TTTGTGAAGA GACCCAGTAA TTTGGCAGTG 
ACTGTGGATG ACAGTGCAGA ATTTAAATGT GAGGCCCGAG GAGACCCTGT GCCTACAGTT 
CGATGGAGGA AAGATGACGG AGAGCTGCCC AAATCCAGAT ATGAAATCCG AGATGATCAT 
ACTTTGAAAA TTCGGAAGGT GACTGCTGGC GACATGGGAT CATACACTTG TGTGGCAGAA 
AATATGGTCG GGAAAGCTGA AGCTTCCGCT ACTTTGACAG TTCAAGTTGG GTCTGAGCCG 
CCACATTTCG TTGTAAAACC TCGGGACCAG GTGGTTGCCT TGGGAAGAAC TGTGACGTTT 
CAGTGCGAAG CAACTGGAAA TCCTCAACCA GCTATCTTTT GGAGGAGAGA GGGGAGTCAG 
AATCTACTTT TCTCCTACCA GCCTCCACAG TCCTCTAGCC GATTTTCAGT TTCGCAGACA 
GGAGACCTCA CCATTACGAA CGTCCAGCGG TCTGATGTCG GCTACTACAT CTGTCAGACT 
TTAAATGTTG CTGGAAGTAT CATCACAAAG GCCTATTTGG AAGTTACTGA TGTGATTGCA 
GACCGGCCTC CCCCAGTCAT TCGGCAAGGT CCTGTGAATC AGACTGTAGC AGTGGATGGC 
ACTTTAATCC TCAGCTGTGT GGCCACAGGC AGTCCAGCAC CCACAATTCT GTGGAGGAAG 
GATGGAGTCC TTGTTTCAAC CCAAGATTCT CGGATCAAAC AGCTGGAGAG TGGCGTGCTG 
CAGATCCGCT ACGCTAAGCT GGGTGACACG GGTCGGTACA CCTGCACCGC ATCGACCCCC 
AGCGGTGAGG CCACGTGGAG CGCTTACATC GAAGTCCAGG AATTTGGGGT TCCAGTTCAA 
CCCCCAAGAC CCACCGACCC CAACTTAATC CCCAGCGCCC CCTCAAAGCC CGAAGTTACA 
GATGTCAGCA AAAACACGGT CACTTTATCG TGGCAGCCAA ACTTGAACTC AGGAGCAACC 
CCAACATCTT ATATTATAGA AGCCTTCAGC CATGCATCTG GTAGCAGCTG GCAGACGGCG 
GCAGAAAATG TGAAAACAGA AACATTTGCC ATCAAAGGAC TCAAACCTAA CGCCATTTAC 
CTTTTCCTGG TGAGGGCAGC AAATGCCTAT GGAATTAGTG ATCCAAGCCA AATATCAGAT 
CCAGTGAAAA CGCAAGATGT CCCACCAACC AGCCAAGGGG TGGACCACAA GCAGGTCCAA 
AGAGAACTGG GGAATGTTGT CTTACATCTG CACAACCCGA CCATCCTTTC TTCCTCCTCA 
GTCGAAGTGC ACTGGACGGT GGACCAACAA TCTCAATATA TTCAAGGCTA TAAAATTCTC 
TACCGCCCAT CTGGAGCCAG CCATGGTGAA TCGGAGTGGT TAGTTTTTGA AGTGAGGACG 
CCAACGAAAA ACAGCGTGGT GATCCCCGAT CTCAGAAAAG GCGTCAACTA CGAGATTAAG 
GCTCGCCCAT TTTTTAATGA GTTTCAAGGA GCAGACAGTG AAATCAAATT TGCCAAAACC 
TTAGAAGAAG CACCAAGTGC CCCTCCCCGA AGTGTAACTG TATCCAAGAA TGATGGAAAT 
GGGACTGCAA TCCTGGTCAC TTGGCAACCG CCTCCTGAAG ACACACAAAA TGGAATGGTC 
CAAGAATATA AGGTTTGGTG TCTCGGTAAT GAAACGAAGT ATCACATAAA CAAGACAGTC 
GATGGCTCGA CCTTCTCGGT GGTCATCCCC TCTCTGGTTC CTGGGATCCG ATACAGTGTG 
GAGGTGGCAG CGAGCACTGG GGCGGGTCCC GGGGTGAAGA GCGAACCTCA GTTCATCCAG 
TTAGATTCTC ACGGAAACCC CGTGTCTCCC GAGGACCAAG TCAGCCTTGC TCAGCAGATC 
TCCGACGTGG TGAGGCAGCC GGCGTTCATT GCAGGCATTG GGGCAGCCTG TTGGATTATC 
CTCATGGTCT TCAGCATCTG GCTTTACAGA CACCGCAAGA AGAGAAACGG ACTCACCAGC 
ACGTATGCGG GTATCCGGAA AGTCCCGTCC TTTACCTTCA CGCCAACAGT AACTTATCAA 
AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG AGGCCGGGAC TCCTCAACAT TAGTGAACCT 
GCCACGCAGC CATGGCTGGC AGACACGTGG CCCAACACAG GCAACAACCA CAATGACTGC 
TCCATCAACT GCTGCACGGC CGGCAATGGC AACAGCGACA GCAACCTGAC AACCTACAGT 
CGCCCAGCTG ATTGTATAGC CAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG 
ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG 
AAAACCTTCA ATAGCCCAAA TCTGAAGGAC GGGCGTTTTG TCAATCCATC AGGGCAGCCC 
ACTCCCTATG CCACCACTCA ACTCATCCAG GCGAACCTCA GCAACAATAT GAATAATGGC 
GCCGGGGACT CCAGCGAGAA GCACTGGAAG CCTCCGGGAC AGCAGAAACC GGAAGTGGCA 
CCAATTCAGT ATAACATCAT GGAGCAAAAC AAGCTGAACA AAGATTATCG GGCAAATGAC 
ACGATCCCTC CAACCATCCC ATACAACCAG TCCTATGACC AGAACACAGG AGGGTCTTAT 
AACAGCTCAG ATCGGGGCAG TAGTACATCC GGGAGTCAAG GCCACAAGAA AGGGGCACGG 
ACACCAAAGG CACCCAAGCA GGGTGGCATG AACTGGGCAG ACTTGCTGCC CCCTCCTCCG 
GCGCATCCTC CCCCACATAG CAACAGTGAA GAATACAACA TGTCCGTAGA TGAAAGCTAT 
GATCAAGAAA TGCCCTGTCC AGTGCCACCT GCGCGGATGT ATTTGCAACA GGATGAATTA 
GAGGAAGAGG AAGATGAAAG AGGCCCCACT CCCCCTGTCC GGGGGGCAGC TTCTTCTCCA 
GCTGCTGTGT CCTACAGCCA CCAATCCACG GCCACTCTCA CCCCTTCTCC CCAGGAAGAG 
CTCCAGCCCA TGTTACAGGA TTGTCCGGAG GACCTCGGGC ACATGCCCCA CCCACCAGAC 
AGGAGGCGGC AGCCCGTGAG TCCTCCTCCA CCGCCACGGC CAATTTCCCC ACCTCATACT 
TACGGCTACA TTTCAGGACC CCTTGTCTCA GATATGGATA CCGATGCCCC AGAAGAGGAG 
GAGGACGAAG CTGACATGGA AGTTGCCAAA ATGCAGACCC GAAGGCTGTT GTTACGTGGG 
CTGGAGCAGA CCCCAGCATC CAGTGTTGGG GACCTTGAGA GCTCCGTCAC TGGGTCCATG 
ATCAATGGCT GGGGCTCAGC CTCGGAAGAG GACAACATTT CCAGTGGGCG CTCCAGTGTC 
AGTTCATCCG ATGGCTCCTT TTTCACTGAC GCTGATTTTG CCCAGGCAGT TGCTGCAGCT 
GCGGAGTATG CGGGCCTGAA AGTGGCTCGC CGCCAAATGC AAGATGCTGC TGGCCGCCGC 
CACTTCCATG CCTCTCAGTG CCCAAGGCCC ACGAGTCCTG TGTCCACAGA CAGCAACATG 
AGTGCTGTTG TGATCCAGAA AGCCAGACCC GCCAAGAAGC AGAAACACCA GCCAGGACAT 
CTGCGCAGGG AAGCCTACGC AGATGATCTT CCACCCCCTC CAGTGCCACC ACCTGCTATA 
AAATCGCCCA CTGTCCAGTC CAAGGCACAG CTGGAGGTAC GGCCTGTCAT GGTGCCAAAA 
CTCGCGTCTA TAGAAGCAAG GACAGATAGA TCGTCAGACA GAAAAGGAGG CAGTTACAAG 
GGGAGAGAAG CTCTGGATGG AAGACAAGTC ACTGACCTGC GAACAAATCC AAGTGACCCC 
AGAGAGGCAC AGGAACAGCC AAATGACGGG AAAGGCCGAG GAACAAAGGC AGCCAAGCGA 
GACCTTCCAC CAGCAAAGAC GCACCTCGGC CAAGAGGATA TTCTTCCGTA CTGTAGACCT 
ACTTTCCCAA CGTCAAACAA CCCTAGAGAC CCCAGTTCCT CCAGCTCTAT GTCATCAAGA 
GGATCAGGGA GCCGACAAAG AGAGCAAGCA AATGTGGGCC GAAGGAATAT GGCAGAAATG 
CAAGTACTTG GGGGATTTGA AAGAGGAGAT GAGAATAATG AAGAATTAGA GGAAACTGAA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172383.1
CDS	124..5109
Translation	MAVLLLMLLFSMAEITSPVGVSGRMRAGSRWRGEKNHPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_017316894.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316894.1

ORF Insert Sequence:

ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCA CCCTGAGGAC 
CTAGAGAGGG GGAACGACAA CGGAACGCCA GCCCCCACCT CTGATAACGA TGACAATTCG 
CTGGGCTACA CAGGCTCCCG TCTTCGTCAG GAAGATTTTC CACCCCGCAT TGTTGAACAC 
CCTTCAGACC TGATCGTCTC CAAAGGAGAA CCTGCCACTC TGAACTGTAA AGCCGAAGGC 
CGCCCCACCC CGACGATAGA ATGGTACAAA GGTGGGGAAA GAGTGGAGAC GGACAAAGAT 
GACCCGCGCT CACACCGGAT GCTGCTGCCA AGCGGGTCTT TATTTTTCTT ACGCATAGTC 
CATGGACGGA AGAGTAGGCC CGACGAAGGG GTCTACATCT GTGTGGCTAG GAATTACCTC 
GGAGAGGCTG TGAGCCACAA CGCATCGCTG GAGGTAGCTA TACTACGGGA TGACTTCAGA 
CAAAACCCTT CAGATGTCAT GGTGGCAGTA GGCGAACCTG CAGTCATGGA ATGCCAGCCT 
CCACGGGGCC ATCCAGAGCC CACCATCTCC TGGAAAAAGG ATGGCTCACC CCTGGATGAT 
AAAGATGAAA GAATCACTAT ACGAGGAGGA AAACTCATGA TCACTTATAC GCGTAAAAGC 
GATGCTGGCA AGTATGTTTG CGTTGGCACC AACATGGTGG GGGAACGGGA GAGTGAAGTC 
GCAGAGCTAA CCGTTTTAGA GAGACCATCG TTTGTGAAGA GACCCAGTAA TTTGGCAGTG 
ACTGTGGATG ACAGTGCAGA ATTTAAATGT GAGGCCCGAG GAGACCCTGT GCCTACAGTT 
CGATGGAGGA AAGATGACGG AGAGCTGCCC AAATCCAGAT ATGAAATCCG AGATGATCAT 
ACTTTGAAAA TTCGGAAGGT GACTGCTGGC GACATGGGAT CATACACTTG TGTGGCAGAA 
AATATGGTCG GGAAAGCTGA AGCTTCCGCT ACTTTGACAG TTCAAGTTGG GTCTGAGCCG 
CCACATTTCG TTGTAAAACC TCGGGACCAG GTGGTTGCCT TGGGAAGAAC TGTGACGTTT 
CAGTGCGAAG CAACTGGAAA TCCTCAACCA GCTATCTTTT GGAGGAGAGA GGGGAGTCAG 
AATCTACTTT TCTCCTACCA GCCTCCACAG TCCTCTAGCC GATTTTCAGT TTCGCAGACA 
GGAGACCTCA CCATTACGAA CGTCCAGCGG TCTGATGTCG GCTACTACAT CTGTCAGACT 
TTAAATGTTG CTGGAAGTAT CATCACAAAG GCCTATTTGG AAGTTACTGA TGTGATTGCA 
GACCGGCCTC CCCCAGTCAT TCGGCAAGGT CCTGTGAATC AGACTGTAGC AGTGGATGGC 
ACTTTAATCC TCAGCTGTGT GGCCACAGGC AGTCCAGCAC CCACAATTCT GTGGAGGAAG 
GATGGAGTCC TTGTTTCAAC CCAAGATTCT CGGATCAAAC AGCTGGAGAG TGGCGTGCTG 
CAGATCCGCT ACGCTAAGCT GGGTGACACG GGTCGGTACA CCTGCACCGC ATCGACCCCC 
AGCGGTGAGG CCACGTGGAG CGCTTACATC GAAGTCCAGG AATTTGGGGT TCCAGTTCAA 
CCCCCAAGAC CCACCGACCC CAACTTAATC CCCAGCGCCC CCTCAAAGCC CGAAGTTACA 
GATGTCAGCA AAAACACGGT CACTTTATCG TGGCAGCCAA ACTTGAACTC AGGAGCAACC 
CCAACATCTT ATATTATAGA AGCCTTCAGC CATGCATCTG GTAGCAGCTG GCAGACGGCG 
GCAGAAAATG TGAAAACAGA AACATTTGCC ATCAAAGGAC TCAAACCTAA CGCCATTTAC 
CTTTTCCTGG TGAGGGCAGC AAATGCCTAT GGAATTAGTG ATCCAAGCCA AATATCAGAT 
CCAGTGAAAA CGCAAGATGT CCCACCAACC AGCCAAGGGG TGGACCACAA GCAGGTCCAA 
AGAGAACTGG GGAATGTTGT CTTACATCTG CACAACCCGA CCATCCTTTC TTCCTCCTCA 
GTCGAAGTGC ACTGGACGGT GGACCAACAA TCTCAATATA TTCAAGGCTA TAAAATTCTC 
TACCGCCCAT CTGGAGCCAG CCATGGTGAA TCGGAGTGGT TAGTTTTTGA AGTGAGGACG 
CCAACGAAAA ACAGCGTGGT GATCCCCGAT CTCAGAAAAG GCGTCAACTA CGAGATTAAG 
GCTCGCCCAT TTTTTAATGA GTTTCAAGGA GCAGACAGTG AAATCAAATT TGCCAAAACC 
TTAGAAGAAG CACCAAGTGC CCCTCCCCGA AGTGTAACTG TATCCAAGAA TGATGGAAAT 
GGGACTGCAA TCCTGGTCAC TTGGCAACCG CCTCCTGAAG ACACACAAAA TGGAATGGTC 
CAAGAATATA AGGTTTGGTG TCTCGGTAAT GAAACGAAGT ATCACATAAA CAAGACAGTC 
GATGGCTCGA CCTTCTCGGT GGTCATCCCC TCTCTGGTTC CTGGGATCCG ATACAGTGTG 
GAGGTGGCAG CGAGCACTGG GGCGGGTCCC GGGGTGAAGA GCGAACCTCA GTTCATCCAG 
TTAGATTCTC ACGGAAACCC CGTGTCTCCC GAGGACCAAG TCAGCCTTGC TCAGCAGATC 
TCCGACGTGG TGAGGCAGCC GGCGTTCATT GCAGGCATTG GGGCAGCCTG TTGGATTATC 
CTCATGGTCT TCAGCATCTG GCTTTACAGA CACCGCAAGA AGAGAAACGG ACTCACCAGC 
ACGTATGCGG GTATCCGGAA AGTCCCGTCC TTTACCTTCA CGCCAACAGT AACTTATCAA 
AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG AGGCCGGGAC TCCTCAACAT TAGTGAACCT 
GCCACGCAGC CATGGCTGGC AGACACGTGG CCCAACACAG GCAACAACCA CAATGACTGC 
TCCATCAACT GCTGCACGGC CGGCAATGGC AACAGCGACA GCAACCTGAC AACCTACAGT 
CGCCCAGCTG ATTGTATAGC CAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG 
ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG 
AAAACCTTCA ATAGCCCAAA TCTGAAGGAC GGGCGTTTTG TCAATCCATC AGGGCAGCCC 
ACTCCCTATG CCACCACTCA ACTCATCCAG GCGAACCTCA GCAACAATAT GAATAATGGC 
GCCGGGGACT CCAGCGAGAA GCACTGGAAG CCTCCGGGAC AGCAGAAACC GGAAGTGGCA 
CCAATTCAGT ATAACATCAT GGAGCAAAAC AAGCTGAACA AAGATTATCG GGCAAATGAC 
ACGATCCCTC CAACCATCCC ATACAACCAG TCCTATGACC AGAACACAGG AGGGTCTTAT 
AACAGCTCAG ATCGGGGCAG TAGTACATCC GGGAGTCAAG GCCACAAGAA AGGGGCACGG 
ACACCAAAGG CACCCAAGCA GGGTGGCATG AACTGGGCAG ACTTGCTGCC CCCTCCTCCG 
GCGCATCCTC CCCCACATAG CAACAGTGAA GAATACAACA TGTCCGTAGA TGAAAGCTAT 
GATCAAGAAA TGCCCTGTCC AGTGCCACCT GCGCGGATGT ATTTGCAACA GGATGAATTA 
GAGGAAGAGG AAGATGAAAG AGGCCCCACT CCCCCTGTCC GGGGGGCAGC TTCTTCTCCA 
GCTGCTGTGT CCTACAGCCA CCAATCCACG GCCACTCTCA CCCCTTCTCC CCAGGAAGAG 
CTCCAGCCCA TGTTACAGGA TTGTCCGGAG GACCTCGGGC ACATGCCCCA CCCACCAGAC 
AGGAGGCGGC AGCCCGTGAG TCCTCCTCCA CCGCCACGGC CAATTTCCCC ACCTCATACT 
TACGGCTACA TTTCAGGACC CCTTGTCTCA GATATGGATA CCGATGCCCC AGAAGAGGAG 
GAGGACGAAG CTGACATGGA AGTTGCCAAA ATGCAGACCC GAAGGCTGTT GTTACGTGGG 
CTGGAGCAGA CCCCAGCATC CAGTGTTGGG GACCTTGAGA GCTCCGTCAC TGGGTCCATG 
ATCAATGGCT GGGGCTCAGC CTCGGAAGAG GACAACATTT CCAGTGGGCG CTCCAGTGTC 
AGTTCATCCG ATGGCTCCTT TTTCACTGAC GCTGATTTTG CCCAGGCAGT TGCTGCAGCT 
GCGGAGTATG CGGGCCTGAA AGTGGCTCGC CGCCAAATGC AAGATGCTGC TGGCCGCCGC 
CACTTCCATG CCTCTCAGTG CCCAAGGCCC ACGAGTCCTG TGTCCACAGA CAGCAACATG 
AGTGCTGTTG TGATCCAGAA AGCCAGACCC GCCAAGAAGC AGAAACACCA GCCAGGACAT 
CTGCGCAGGG AAGCCTACGC AGATGATCTT CCACCCCCTC CAGTGCCACC ACCTGCTATA 
AAATCGCCCA CTGTCCAGTC CAAGGCACAG CTGGAGGTAC GGCCTGTCAT GGTGCCAAAA 
CTCGCGTCTA TAGAAGCAAG GACAGATAGA TCGTCAGACA GAAAAGGAGG CAGTTACAAG 
GGGAGAGAAG CTCTGGATGG AAGACAAGTC ACTGACCTGC GAACAAATCC AAGTGACCCC 
AGAGAGGCAC AGGAACAGCC AAATGACGGG AAAGGCCGAG GAACAAAGGC AGCCAAGCGA 
GACCTTCCAC CAGCAAAGAC GCACCTCGGC CAAGAGGATA TTCTTCCGTA CTGTAGACCT 
ACTTTCCCAA CGTCAAACAA CCCTAGAGAC CCCAGTTCCT CCAGCTCTAT GTCATCAAGA 
GGATCAGGGA GCCGACAAAG AGAGCAAGCA AATGTGGGCC GAAGGAATAT GGCAGAAATG 
CAAGTACTTG GGGGATTTGA AAGAGGAGAT GAGAATAATG AAGAATTAGA GGAAACTGAA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_017316894.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172383.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu79278
Clone ID Related Accession (Same CDS sequence)	XM_017316894.1 , XM_017316894.2
Accession Version	XM_017316894.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCA CCCTGAGGAC 
CTAGAGAGGG GGAACGACAA CGGAACGCCA GCCCCCACCT CTGATAACGA TGACAATTCG 
CTGGGCTACA CAGGCTCCCG TCTTCGTCAG GAAGATTTTC CACCCCGCAT TGTTGAACAC 
CCTTCAGACC TGATCGTCTC CAAAGGAGAA CCTGCCACTC TGAACTGTAA AGCCGAAGGC 
CGCCCCACCC CGACGATAGA ATGGTACAAA GGTGGGGAAA GAGTGGAGAC GGACAAAGAT 
GACCCGCGCT CACACCGGAT GCTGCTGCCA AGCGGGTCTT TATTTTTCTT ACGCATAGTC 
CATGGACGGA AGAGTAGGCC CGACGAAGGG GTCTACATCT GTGTGGCTAG GAATTACCTC 
GGAGAGGCTG TGAGCCACAA CGCATCGCTG GAGGTAGCTA TACTACGGGA TGACTTCAGA 
CAAAACCCTT CAGATGTCAT GGTGGCAGTA GGCGAACCTG CAGTCATGGA ATGCCAGCCT 
CCACGGGGCC ATCCAGAGCC CACCATCTCC TGGAAAAAGG ATGGCTCACC CCTGGATGAT 
AAAGATGAAA GAATCACTAT ACGAGGAGGA AAACTCATGA TCACTTATAC GCGTAAAAGC 
GATGCTGGCA AGTATGTTTG CGTTGGCACC AACATGGTGG GGGAACGGGA GAGTGAAGTC 
GCAGAGCTAA CCGTTTTAGA GAGACCATCG TTTGTGAAGA GACCCAGTAA TTTGGCAGTG 
ACTGTGGATG ACAGTGCAGA ATTTAAATGT GAGGCCCGAG GAGACCCTGT GCCTACAGTT 
CGATGGAGGA AAGATGACGG AGAGCTGCCC AAATCCAGAT ATGAAATCCG AGATGATCAT 
ACTTTGAAAA TTCGGAAGGT GACTGCTGGC GACATGGGAT CATACACTTG TGTGGCAGAA 
AATATGGTCG GGAAAGCTGA AGCTTCCGCT ACTTTGACAG TTCAAGTTGG GTCTGAGCCG 
CCACATTTCG TTGTAAAACC TCGGGACCAG GTGGTTGCCT TGGGAAGAAC TGTGACGTTT 
CAGTGCGAAG CAACTGGAAA TCCTCAACCA GCTATCTTTT GGAGGAGAGA GGGGAGTCAG 
AATCTACTTT TCTCCTACCA GCCTCCACAG TCCTCTAGCC GATTTTCAGT TTCGCAGACA 
GGAGACCTCA CCATTACGAA CGTCCAGCGG TCTGATGTCG GCTACTACAT CTGTCAGACT 
TTAAATGTTG CTGGAAGTAT CATCACAAAG GCCTATTTGG AAGTTACTGA TGTGATTGCA 
GACCGGCCTC CCCCAGTCAT TCGGCAAGGT CCTGTGAATC AGACTGTAGC AGTGGATGGC 
ACTTTAATCC TCAGCTGTGT GGCCACAGGC AGTCCAGCAC CCACAATTCT GTGGAGGAAG 
GATGGAGTCC TTGTTTCAAC CCAAGATTCT CGGATCAAAC AGCTGGAGAG TGGCGTGCTG 
CAGATCCGCT ACGCTAAGCT GGGTGACACG GGTCGGTACA CCTGCACCGC ATCGACCCCC 
AGCGGTGAGG CCACGTGGAG CGCTTACATC GAAGTCCAGG AATTTGGGGT TCCAGTTCAA 
CCCCCAAGAC CCACCGACCC CAACTTAATC CCCAGCGCCC CCTCAAAGCC CGAAGTTACA 
GATGTCAGCA AAAACACGGT CACTTTATCG TGGCAGCCAA ACTTGAACTC AGGAGCAACC 
CCAACATCTT ATATTATAGA AGCCTTCAGC CATGCATCTG GTAGCAGCTG GCAGACGGCG 
GCAGAAAATG TGAAAACAGA AACATTTGCC ATCAAAGGAC TCAAACCTAA CGCCATTTAC 
CTTTTCCTGG TGAGGGCAGC AAATGCCTAT GGAATTAGTG ATCCAAGCCA AATATCAGAT 
CCAGTGAAAA CGCAAGATGT CCCACCAACC AGCCAAGGGG TGGACCACAA GCAGGTCCAA 
AGAGAACTGG GGAATGTTGT CTTACATCTG CACAACCCGA CCATCCTTTC TTCCTCCTCA 
GTCGAAGTGC ACTGGACGGT GGACCAACAA TCTCAATATA TTCAAGGCTA TAAAATTCTC 
TACCGCCCAT CTGGAGCCAG CCATGGTGAA TCGGAGTGGT TAGTTTTTGA AGTGAGGACG 
CCAACGAAAA ACAGCGTGGT GATCCCCGAT CTCAGAAAAG GCGTCAACTA CGAGATTAAG 
GCTCGCCCAT TTTTTAATGA GTTTCAAGGA GCAGACAGTG AAATCAAATT TGCCAAAACC 
TTAGAAGAAG CACCAAGTGC CCCTCCCCGA AGTGTAACTG TATCCAAGAA TGATGGAAAT 
GGGACTGCAA TCCTGGTCAC TTGGCAACCG CCTCCTGAAG ACACACAAAA TGGAATGGTC 
CAAGAATATA AGGTTTGGTG TCTCGGTAAT GAAACGAAGT ATCACATAAA CAAGACAGTC 
GATGGCTCGA CCTTCTCGGT GGTCATCCCC TCTCTGGTTC CTGGGATCCG ATACAGTGTG 
GAGGTGGCAG CGAGCACTGG GGCGGGTCCC GGGGTGAAGA GCGAACCTCA GTTCATCCAG 
TTAGATTCTC ACGGAAACCC CGTGTCTCCC GAGGACCAAG TCAGCCTTGC TCAGCAGATC 
TCCGACGTGG TGAGGCAGCC GGCGTTCATT GCAGGCATTG GGGCAGCCTG TTGGATTATC 
CTCATGGTCT TCAGCATCTG GCTTTACAGA CACCGCAAGA AGAGAAACGG ACTCACCAGC 
ACGTATGCGG GTATCCGGAA AGTCCCGTCC TTTACCTTCA CGCCAACAGT AACTTATCAA 
AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG AGGCCGGGAC TCCTCAACAT TAGTGAACCT 
GCCACGCAGC CATGGCTGGC AGACACGTGG CCCAACACAG GCAACAACCA CAATGACTGC 
TCCATCAACT GCTGCACGGC CGGCAATGGC AACAGCGACA GCAACCTGAC AACCTACAGT 
CGCCCAGCTG ATTGTATAGC CAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG 
ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG 
AAAACCTTCA ATAGCCCAAA TCTGAAGGAC GGGCGTTTTG TCAATCCATC AGGGCAGCCC 
ACTCCCTATG CCACCACTCA ACTCATCCAG GCGAACCTCA GCAACAATAT GAATAATGGC 
GCCGGGGACT CCAGCGAGAA GCACTGGAAG CCTCCGGGAC AGCAGAAACC GGAAGTGGCA 
CCAATTCAGT ATAACATCAT GGAGCAAAAC AAGCTGAACA AAGATTATCG GGCAAATGAC 
ACGATCCCTC CAACCATCCC ATACAACCAG TCCTATGACC AGAACACAGG AGGGTCTTAT 
AACAGCTCAG ATCGGGGCAG TAGTACATCC GGGAGTCAAG GCCACAAGAA AGGGGCACGG 
ACACCAAAGG CACCCAAGCA GGGTGGCATG AACTGGGCAG ACTTGCTGCC CCCTCCTCCG 
GCGCATCCTC CCCCACATAG CAACAGTGAA GAATACAACA TGTCCGTAGA TGAAAGCTAT 
GATCAAGAAA TGCCCTGTCC AGTGCCACCT GCGCGGATGT ATTTGCAACA GGATGAATTA 
GAGGAAGAGG AAGATGAAAG AGGCCCCACT CCCCCTGTCC GGGGGGCAGC TTCTTCTCCA 
GCTGCTGTGT CCTACAGCCA CCAATCCACG GCCACTCTCA CCCCTTCTCC CCAGGAAGAG 
CTCCAGCCCA TGTTACAGGA TTGTCCGGAG GACCTCGGGC ACATGCCCCA CCCACCAGAC 
AGGAGGCGGC AGCCCGTGAG TCCTCCTCCA CCGCCACGGC CAATTTCCCC ACCTCATACT 
TACGGCTACA TTTCAGGACC CCTTGTCTCA GATATGGATA CCGATGCCCC AGAAGAGGAG 
GAGGACGAAG CTGACATGGA AGTTGCCAAA ATGCAGACCC GAAGGCTGTT GTTACGTGGG 
CTGGAGCAGA CCCCAGCATC CAGTGTTGGG GACCTTGAGA GCTCCGTCAC TGGGTCCATG 
ATCAATGGCT GGGGCTCAGC CTCGGAAGAG GACAACATTT CCAGTGGGCG CTCCAGTGTC 
AGTTCATCCG ATGGCTCCTT TTTCACTGAC GCTGATTTTG CCCAGGCAGT TGCTGCAGCT 
GCGGAGTATG CGGGCCTGAA AGTGGCTCGC CGCCAAATGC AAGATGCTGC TGGCCGCCGC 
CACTTCCATG CCTCTCAGTG CCCAAGGCCC ACGAGTCCTG TGTCCACAGA CAGCAACATG 
AGTGCTGTTG TGATCCAGAA AGCCAGACCC GCCAAGAAGC AGAAACACCA GCCAGGACAT 
CTGCGCAGGG AAGCCTACGC AGATGATCTT CCACCCCCTC CAGTGCCACC ACCTGCTATA 
AAATCGCCCA CTGTCCAGTC CAAGGCACAG CTGGAGGTAC GGCCTGTCAT GGTGCCAAAA 
CTCGCGTCTA TAGAAGCAAG GACAGATAGA TCGTCAGACA GAAAAGGAGG CAGTTACAAG 
GGGAGAGAAG CTCTGGATGG AAGACAAGTC ACTGACCTGC GAACAAATCC AAGTGACCCC 
AGAGAGGCAC AGGAACAGCC AAATGACGGG AAAGGCCGAG GAACAAAGGC AGCCAAGCGA 
GACCTTCCAC CAGCAAAGAC GCACCTCGGC CAAGAGGATA TTCTTCCGTA CTGTAGACCT 
ACTTTCCCAA CGTCAAACAA CCCTAGAGAC CCCAGTTCCT CCAGCTCTAT GTCATCAAGA 
GGATCAGGGA GCCGACAAAG AGAGCAAGCA AATGTGGGCC GAAGGAATAT GGCAGAAATG 
CAAGTACTTG GGGGATTTGA AAGAGGAGAT GAGAATAATG AAGAATTAGA GGAAACTGAA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172383.1
CDS	473..5458
Translation	MAVLLLMLLFSMAEITSPVGVSGRMRAGSRWRGEKNHPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_017316894.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316894.2

ORF Insert Sequence:

ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCA CCCTGAGGAC 
CTAGAGAGGG GGAACGACAA CGGAACGCCA GCCCCCACCT CTGATAACGA TGACAATTCG 
CTGGGCTACA CAGGCTCCCG TCTTCGTCAG GAAGATTTTC CACCCCGCAT TGTTGAACAC 
CCTTCAGACC TGATCGTCTC CAAAGGAGAA CCTGCCACTC TGAACTGTAA AGCCGAAGGC 
CGCCCCACCC CGACGATAGA ATGGTACAAA GGTGGGGAAA GAGTGGAGAC GGACAAAGAT 
GACCCGCGCT CACACCGGAT GCTGCTGCCA AGCGGGTCTT TATTTTTCTT ACGCATAGTC 
CATGGACGGA AGAGTAGGCC CGACGAAGGG GTCTACATCT GTGTGGCTAG GAATTACCTC 
GGAGAGGCTG TGAGCCACAA CGCATCGCTG GAGGTAGCTA TACTACGGGA TGACTTCAGA 
CAAAACCCTT CAGATGTCAT GGTGGCAGTA GGCGAACCTG CAGTCATGGA ATGCCAGCCT 
CCACGGGGCC ATCCAGAGCC CACCATCTCC TGGAAAAAGG ATGGCTCACC CCTGGATGAT 
AAAGATGAAA GAATCACTAT ACGAGGAGGA AAACTCATGA TCACTTATAC GCGTAAAAGC 
GATGCTGGCA AGTATGTTTG CGTTGGCACC AACATGGTGG GGGAACGGGA GAGTGAAGTC 
GCAGAGCTAA CCGTTTTAGA GAGACCATCG TTTGTGAAGA GACCCAGTAA TTTGGCAGTG 
ACTGTGGATG ACAGTGCAGA ATTTAAATGT GAGGCCCGAG GAGACCCTGT GCCTACAGTT 
CGATGGAGGA AAGATGACGG AGAGCTGCCC AAATCCAGAT ATGAAATCCG AGATGATCAT 
ACTTTGAAAA TTCGGAAGGT GACTGCTGGC GACATGGGAT CATACACTTG TGTGGCAGAA 
AATATGGTCG GGAAAGCTGA AGCTTCCGCT ACTTTGACAG TTCAAGTTGG GTCTGAGCCG 
CCACATTTCG TTGTAAAACC TCGGGACCAG GTGGTTGCCT TGGGAAGAAC TGTGACGTTT 
CAGTGCGAAG CAACTGGAAA TCCTCAACCA GCTATCTTTT GGAGGAGAGA GGGGAGTCAG 
AATCTACTTT TCTCCTACCA GCCTCCACAG TCCTCTAGCC GATTTTCAGT TTCGCAGACA 
GGAGACCTCA CCATTACGAA CGTCCAGCGG TCTGATGTCG GCTACTACAT CTGTCAGACT 
TTAAATGTTG CTGGAAGTAT CATCACAAAG GCCTATTTGG AAGTTACTGA TGTGATTGCA 
GACCGGCCTC CCCCAGTCAT TCGGCAAGGT CCTGTGAATC AGACTGTAGC AGTGGATGGC 
ACTTTAATCC TCAGCTGTGT GGCCACAGGC AGTCCAGCAC CCACAATTCT GTGGAGGAAG 
GATGGAGTCC TTGTTTCAAC CCAAGATTCT CGGATCAAAC AGCTGGAGAG TGGCGTGCTG 
CAGATCCGCT ACGCTAAGCT GGGTGACACG GGTCGGTACA CCTGCACCGC ATCGACCCCC 
AGCGGTGAGG CCACGTGGAG CGCTTACATC GAAGTCCAGG AATTTGGGGT TCCAGTTCAA 
CCCCCAAGAC CCACCGACCC CAACTTAATC CCCAGCGCCC CCTCAAAGCC CGAAGTTACA 
GATGTCAGCA AAAACACGGT CACTTTATCG TGGCAGCCAA ACTTGAACTC AGGAGCAACC 
CCAACATCTT ATATTATAGA AGCCTTCAGC CATGCATCTG GTAGCAGCTG GCAGACGGCG 
GCAGAAAATG TGAAAACAGA AACATTTGCC ATCAAAGGAC TCAAACCTAA CGCCATTTAC 
CTTTTCCTGG TGAGGGCAGC AAATGCCTAT GGAATTAGTG ATCCAAGCCA AATATCAGAT 
CCAGTGAAAA CGCAAGATGT CCCACCAACC AGCCAAGGGG TGGACCACAA GCAGGTCCAA 
AGAGAACTGG GGAATGTTGT CTTACATCTG CACAACCCGA CCATCCTTTC TTCCTCCTCA 
GTCGAAGTGC ACTGGACGGT GGACCAACAA TCTCAATATA TTCAAGGCTA TAAAATTCTC 
TACCGCCCAT CTGGAGCCAG CCATGGTGAA TCGGAGTGGT TAGTTTTTGA AGTGAGGACG 
CCAACGAAAA ACAGCGTGGT GATCCCCGAT CTCAGAAAAG GCGTCAACTA CGAGATTAAG 
GCTCGCCCAT TTTTTAATGA GTTTCAAGGA GCAGACAGTG AAATCAAATT TGCCAAAACC 
TTAGAAGAAG CACCAAGTGC CCCTCCCCGA AGTGTAACTG TATCCAAGAA TGATGGAAAT 
GGGACTGCAA TCCTGGTCAC TTGGCAACCG CCTCCTGAAG ACACACAAAA TGGAATGGTC 
CAAGAATATA AGGTTTGGTG TCTCGGTAAT GAAACGAAGT ATCACATAAA CAAGACAGTC 
GATGGCTCGA CCTTCTCGGT GGTCATCCCC TCTCTGGTTC CTGGGATCCG ATACAGTGTG 
GAGGTGGCAG CGAGCACTGG GGCGGGTCCC GGGGTGAAGA GCGAACCTCA GTTCATCCAG 
TTAGATTCTC ACGGAAACCC CGTGTCTCCC GAGGACCAAG TCAGCCTTGC TCAGCAGATC 
TCCGACGTGG TGAGGCAGCC GGCGTTCATT GCAGGCATTG GGGCAGCCTG TTGGATTATC 
CTCATGGTCT TCAGCATCTG GCTTTACAGA CACCGCAAGA AGAGAAACGG ACTCACCAGC 
ACGTATGCGG GTATCCGGAA AGTCCCGTCC TTTACCTTCA CGCCAACAGT AACTTATCAA 
AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG AGGCCGGGAC TCCTCAACAT TAGTGAACCT 
GCCACGCAGC CATGGCTGGC AGACACGTGG CCCAACACAG GCAACAACCA CAATGACTGC 
TCCATCAACT GCTGCACGGC CGGCAATGGC AACAGCGACA GCAACCTGAC AACCTACAGT 
CGCCCAGCTG ATTGTATAGC CAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG 
ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG 
AAAACCTTCA ATAGCCCAAA TCTGAAGGAC GGGCGTTTTG TCAATCCATC AGGGCAGCCC 
ACTCCCTATG CCACCACTCA ACTCATCCAG GCGAACCTCA GCAACAATAT GAATAATGGC 
GCCGGGGACT CCAGCGAGAA GCACTGGAAG CCTCCGGGAC AGCAGAAACC GGAAGTGGCA 
CCAATTCAGT ATAACATCAT GGAGCAAAAC AAGCTGAACA AAGATTATCG GGCAAATGAC 
ACGATCCCTC CAACCATCCC ATACAACCAG TCCTATGACC AGAACACAGG AGGGTCTTAT 
AACAGCTCAG ATCGGGGCAG TAGTACATCC GGGAGTCAAG GCCACAAGAA AGGGGCACGG 
ACACCAAAGG CACCCAAGCA GGGTGGCATG AACTGGGCAG ACTTGCTGCC CCCTCCTCCG 
GCGCATCCTC CCCCACATAG CAACAGTGAA GAATACAACA TGTCCGTAGA TGAAAGCTAT 
GATCAAGAAA TGCCCTGTCC AGTGCCACCT GCGCGGATGT ATTTGCAACA GGATGAATTA 
GAGGAAGAGG AAGATGAAAG AGGCCCCACT CCCCCTGTCC GGGGGGCAGC TTCTTCTCCA 
GCTGCTGTGT CCTACAGCCA CCAATCCACG GCCACTCTCA CCCCTTCTCC CCAGGAAGAG 
CTCCAGCCCA TGTTACAGGA TTGTCCGGAG GACCTCGGGC ACATGCCCCA CCCACCAGAC 
AGGAGGCGGC AGCCCGTGAG TCCTCCTCCA CCGCCACGGC CAATTTCCCC ACCTCATACT 
TACGGCTACA TTTCAGGACC CCTTGTCTCA GATATGGATA CCGATGCCCC AGAAGAGGAG 
GAGGACGAAG CTGACATGGA AGTTGCCAAA ATGCAGACCC GAAGGCTGTT GTTACGTGGG 
CTGGAGCAGA CCCCAGCATC CAGTGTTGGG GACCTTGAGA GCTCCGTCAC TGGGTCCATG 
ATCAATGGCT GGGGCTCAGC CTCGGAAGAG GACAACATTT CCAGTGGGCG CTCCAGTGTC 
AGTTCATCCG ATGGCTCCTT TTTCACTGAC GCTGATTTTG CCCAGGCAGT TGCTGCAGCT 
GCGGAGTATG CGGGCCTGAA AGTGGCTCGC CGCCAAATGC AAGATGCTGC TGGCCGCCGC 
CACTTCCATG CCTCTCAGTG CCCAAGGCCC ACGAGTCCTG TGTCCACAGA CAGCAACATG 
AGTGCTGTTG TGATCCAGAA AGCCAGACCC GCCAAGAAGC AGAAACACCA GCCAGGACAT 
CTGCGCAGGG AAGCCTACGC AGATGATCTT CCACCCCCTC CAGTGCCACC ACCTGCTATA 
AAATCGCCCA CTGTCCAGTC CAAGGCACAG CTGGAGGTAC GGCCTGTCAT GGTGCCAAAA 
CTCGCGTCTA TAGAAGCAAG GACAGATAGA TCGTCAGACA GAAAAGGAGG CAGTTACAAG 
GGGAGAGAAG CTCTGGATGG AAGACAAGTC ACTGACCTGC GAACAAATCC AAGTGACCCC 
AGAGAGGCAC AGGAACAGCC AAATGACGGG AAAGGCCGAG GAACAAAGGC AGCCAAGCGA 
GACCTTCCAC CAGCAAAGAC GCACCTCGGC CAAGAGGATA TTCTTCCGTA CTGTAGACCT 
ACTTTCCCAA CGTCAAACAA CCCTAGAGAC CCCAGTTCCT CCAGCTCTAT GTCATCAAGA 
GGATCAGGGA GCCGACAAAG AGAGCAAGCA AATGTGGGCC GAAGGAATAT GGCAGAAATG 
CAAGTACTTG GGGGATTTGA AAGAGGAGAT GAGAATAATG AAGAATTAGA GGAAACTGAA 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_030249003.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104863.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA.

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CloneID	OMu86897
Clone ID Related Accession (Same CDS sequence)	XM_030249003.1
Accession Version	XM_030249003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGAGCCGC CACATTTCGT TGTAAAACCT CGGGACCAGG TGGTTGCCTT GGGAAGAACT 
GTGACGTTTC AGTGCGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GAGGAGAGAG 
GGGAGTCAGA ATCTACTTTT CTCCTACCAG CCTCCACAGT CCTCTAGCCG ATTTTCAGTT 
TCGCAGACAG GAGACCTCAC CATTACGAAC GTCCAGCGGT CTGATGTCGG CTACTACATC 
TGTCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAGG CCTATTTGGA AGTTACTGAT 
GTGATTGCAG ACCGGCCTCC CCCAGTCATT CGGCAAGGTC CTGTGAATCA GACTGTAGCA 
GTGGATGGCA CTTTAATCCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACAATTCTG 
TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGT 
GGCGTGCTGC AGATCCGCTA CGCTAAGCTG GGTGACACGG GTCGGTACAC CTGCACCGCA 
TCGACCCCCA GCGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGGGTT 
CCAGTTCAAC CCCCAAGACC CACCGACCCC AACTTAATCC CCAGCGCCCC CTCAAAGCCC 
GAAGTTACAG ATGTCAGCAA AAACACGGTC ACTTTATCGT GGCAGCCAAA CTTGAACTCA 
GGAGCAACCC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG 
CAGACGGCGG CAGAAAATGT GAAAACAGAA ACATTTGCCA TCAAAGGACT CAAACCTAAC 
GCCATTTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GAATTAGTGA TCCAAGCCAA 
ATATCAGATC CAGTGAAAAC GCAAGATGTC CCACCAACCA GCCAAGGGGT GGACCACAAG 
CAGGTCCAAA GAGAACTGGG GAATGTTGTC TTACATCTGC ACAACCCGAC CATCCTTTCT 
TCCTCCTCAG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGCTAT 
AAAATTCTCT ACCGCCCATC TGGAGCCAGC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA 
GTGAGGACGC CAACGAAAAA CAGCGTGGTG ATCCCCGATC TCAGAAAAGG CGTCAACTAC 
GAGATTAAGG CTCGCCCATT TTTTAATGAG TTTCAAGGAG CAGACAGTGA AATCAAATTT 
GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTAACTGT ATCCAAGAAT 
GATGGAAATG GGACTGCAAT CCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAAAAT 
GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGTAATG AAACGAAGTA TCACATAAAC 
AAGACAGTCG ATGGCTCGAC CTTCTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGA 
TACAGTGTGG AGGTGGCAGC GAGCACTGGG GCGGGTCCCG GGGTGAAGAG CGAACCTCAG 
TTCATCCAGT TAGATTCTCA CGGAAACCCC GTGTCTCCCG AGGACCAAGT CAGCCTTGCT 
CAGCAGATCT CCGACGTGGT GAGGCAGCCG GCGTTCATTG CAGGCATTGG GGCAGCCTGT 
TGGATTATCC TCATGGTCTT CAGCATCTGG CTTTACAGAC ACCGCAAGAA GAGAAACGGA 
CTCACCAGCA CGTATGCGGG TATCCGGAAA GTCCCGTCCT TTACCTTCAC GCCAACAGTA 
ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATT 
AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CCAACACAGG CAACAACCAC 
AATGACTGCT CCATCAACTG CTGCACGGCC GGCAATGGCA ACAGCGACAG CAACCTGACA 
ACCTACAGTC GCCCAGGGCA GCCCACTCCC TATGCCACCA CTCAACTCAT CCAGGCGAAC 
CTCAGCAACA ATATGAATAA TGGCGCCGGG GACTCCAGCG AGAAGCACTG GAAGCCTCCG 
GGACAGCAGA AACCGGAAGT GGCACCAATT CAGTATAACA TCATGGAGCA AAACAAGCTG 
AACAAAGATT ATCGGGCAAA TGACACGATC CCTCCAACCA TCCCATACAA CCAGTCCTAT 
GACCAGAACA CAGGAGGGTC TTATAACAGC TCAGATCGGG GCAGTAGTAC ATCCGGGAGT 
CAAGGCCACA AGAAAGGGGC ACGGACACCA AAGGCACCCA AGCAGGGTGG CATGAACTGG 
GCAGACTTGC TGCCCCCTCC TCCGGCGCAT CCTCCCCCAC ATAGCAACAG TGAAGAATAC 
AACATGTCCG TAGATGAAAG CTATGATCAA GAAATGCCCT GTCCAGTGCC ACCTGCGCGG 
ATGTATTTGC AACAGGATGA ATTAGAGGAA GAGGAAGATG AAAGAGGCCC CACTCCCCCT 
GTCCGGGGGG CAGCTTCTTC TCCAGCTGCT GTGTCCTACA GCCACCAATC CACGGCCACT 
CTCACCCCTT CTCCCCAGGA AGAGCTCCAG CCCATGTTAC AGGATTGTCC GGAGGACCTC 
GGGCACATGC CCCACCCACC AGACAGGAGG CGGCAGCCCG TGAGTCCTCC TCCACCGCCA 
CGGCCAATTT CCCCACCTCA TACTTACGGC TACATTTCAG GACCCCTTGT CTCAGATATG 
GATACCGATG CCCCAGAAGA GGAGGAGGAC GAAGCTGACA TGGAAGTTGC CAAAATGCAG 
ACCCGAAGGC TGTTGTTACG TGGGCTGGAG CAGACCCCAG CATCCAGTGT TGGGGACCTT 
GAGAGCTCCG TCACTGGGTC CATGATCAAT GGCTGGGGCT CAGCCTCGGA AGAGGACAAC 
ATTTCCAGTG GGCGCTCCAG TGTCAGTTCA TCCGATGGCT CCTTTTTCAC TGACGCTGAT 
TTTGCCCAGG CAGTTGCTGC AGCTGCGGAG TATGCGGGCC TGAAAGTGGC TCGCCGCCAA 
ATGCAAGATG CTGCTGGCCG CCGCCACTTC CATGCCTCTC AGTGCCCAAG GCCCACGAGT 
CCTGTGTCCA CAGACAGCAA CATGAGTGCT GTTGTGATCC AGAAAGCCAG ACCCGCCAAG 
AAGCAGAAAC ACCAGCCAGG ACATCTGCGC AGGGAAGCCT ACGCAGATGA TCTTCCACCC 
CCTCCAGTGC CACCACCTGC TATAAAATCG CCCACTGTCC AGTCCAAGGC ACAGCTGGAG 
GTACGGCCTG TCATGGTGCC AAAACTCGCG TCTATAGAAG CAAGGACAGA TAGATCGTCA 
GACAGAAAAG GAGGCAGTTA CAAGGGGAGA GAAGCTCTGG ATGGAAGACA AGTCACTGAC 
CTGCGAACAA ATCCAAGTGA CCCCAGAGAG GCACAGGAAC AGCCAAATGA CGGGAAAGGC 
CGAGGAACAA AGGCAGCCAA GCGAGACCTT CCACCAGCAA AGACGCACCT CGGCCAAGAG 
GATATTCTTC CGTACTGTAG ACCTACTTTC CCAACGTCAA ACAACCCTAG AGACCCCAGT 
TCCTCCAGCT CTATGTCATC AAGAGGATCA GGGAGCCGAC AAAGAGAGCA AGCAAATGTG 
GGCCGAAGGA ATATGGCAGA AATGCAAGTA CTTGGGGGAT TTGAAAGAGG AGATGAGAAT 
AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104863.1
CDS	97..4986
Translation	MVLRPARGSVCCRKGKEDVCCVLSLCVFLSHVDLLVSGHAVIEMATTVTSVTPSAALVLFPTDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_030249003.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249003.1

ORF Insert Sequence:

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGAGCCGC CACATTTCGT TGTAAAACCT CGGGACCAGG TGGTTGCCTT GGGAAGAACT 
GTGACGTTTC AGTGCGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GAGGAGAGAG 
GGGAGTCAGA ATCTACTTTT CTCCTACCAG CCTCCACAGT CCTCTAGCCG ATTTTCAGTT 
TCGCAGACAG GAGACCTCAC CATTACGAAC GTCCAGCGGT CTGATGTCGG CTACTACATC 
TGTCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAGG CCTATTTGGA AGTTACTGAT 
GTGATTGCAG ACCGGCCTCC CCCAGTCATT CGGCAAGGTC CTGTGAATCA GACTGTAGCA 
GTGGATGGCA CTTTAATCCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACAATTCTG 
TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGT 
GGCGTGCTGC AGATCCGCTA CGCTAAGCTG GGTGACACGG GTCGGTACAC CTGCACCGCA 
TCGACCCCCA GCGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGGGTT 
CCAGTTCAAC CCCCAAGACC CACCGACCCC AACTTAATCC CCAGCGCCCC CTCAAAGCCC 
GAAGTTACAG ATGTCAGCAA AAACACGGTC ACTTTATCGT GGCAGCCAAA CTTGAACTCA 
GGAGCAACCC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG 
CAGACGGCGG CAGAAAATGT GAAAACAGAA ACATTTGCCA TCAAAGGACT CAAACCTAAC 
GCCATTTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GAATTAGTGA TCCAAGCCAA 
ATATCAGATC CAGTGAAAAC GCAAGATGTC CCACCAACCA GCCAAGGGGT GGACCACAAG 
CAGGTCCAAA GAGAACTGGG GAATGTTGTC TTACATCTGC ACAACCCGAC CATCCTTTCT 
TCCTCCTCAG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGCTAT 
AAAATTCTCT ACCGCCCATC TGGAGCCAGC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA 
GTGAGGACGC CAACGAAAAA CAGCGTGGTG ATCCCCGATC TCAGAAAAGG CGTCAACTAC 
GAGATTAAGG CTCGCCCATT TTTTAATGAG TTTCAAGGAG CAGACAGTGA AATCAAATTT 
GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTAACTGT ATCCAAGAAT 
GATGGAAATG GGACTGCAAT CCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAAAAT 
GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGTAATG AAACGAAGTA TCACATAAAC 
AAGACAGTCG ATGGCTCGAC CTTCTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGA 
TACAGTGTGG AGGTGGCAGC GAGCACTGGG GCGGGTCCCG GGGTGAAGAG CGAACCTCAG 
TTCATCCAGT TAGATTCTCA CGGAAACCCC GTGTCTCCCG AGGACCAAGT CAGCCTTGCT 
CAGCAGATCT CCGACGTGGT GAGGCAGCCG GCGTTCATTG CAGGCATTGG GGCAGCCTGT 
TGGATTATCC TCATGGTCTT CAGCATCTGG CTTTACAGAC ACCGCAAGAA GAGAAACGGA 
CTCACCAGCA CGTATGCGGG TATCCGGAAA GTCCCGTCCT TTACCTTCAC GCCAACAGTA 
ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATT 
AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CCAACACAGG CAACAACCAC 
AATGACTGCT CCATCAACTG CTGCACGGCC GGCAATGGCA ACAGCGACAG CAACCTGACA 
ACCTACAGTC GCCCAGGGCA GCCCACTCCC TATGCCACCA CTCAACTCAT CCAGGCGAAC 
CTCAGCAACA ATATGAATAA TGGCGCCGGG GACTCCAGCG AGAAGCACTG GAAGCCTCCG 
GGACAGCAGA AACCGGAAGT GGCACCAATT CAGTATAACA TCATGGAGCA AAACAAGCTG 
AACAAAGATT ATCGGGCAAA TGACACGATC CCTCCAACCA TCCCATACAA CCAGTCCTAT 
GACCAGAACA CAGGAGGGTC TTATAACAGC TCAGATCGGG GCAGTAGTAC ATCCGGGAGT 
CAAGGCCACA AGAAAGGGGC ACGGACACCA AAGGCACCCA AGCAGGGTGG CATGAACTGG 
GCAGACTTGC TGCCCCCTCC TCCGGCGCAT CCTCCCCCAC ATAGCAACAG TGAAGAATAC 
AACATGTCCG TAGATGAAAG CTATGATCAA GAAATGCCCT GTCCAGTGCC ACCTGCGCGG 
ATGTATTTGC AACAGGATGA ATTAGAGGAA GAGGAAGATG AAAGAGGCCC CACTCCCCCT 
GTCCGGGGGG CAGCTTCTTC TCCAGCTGCT GTGTCCTACA GCCACCAATC CACGGCCACT 
CTCACCCCTT CTCCCCAGGA AGAGCTCCAG CCCATGTTAC AGGATTGTCC GGAGGACCTC 
GGGCACATGC CCCACCCACC AGACAGGAGG CGGCAGCCCG TGAGTCCTCC TCCACCGCCA 
CGGCCAATTT CCCCACCTCA TACTTACGGC TACATTTCAG GACCCCTTGT CTCAGATATG 
GATACCGATG CCCCAGAAGA GGAGGAGGAC GAAGCTGACA TGGAAGTTGC CAAAATGCAG 
ACCCGAAGGC TGTTGTTACG TGGGCTGGAG CAGACCCCAG CATCCAGTGT TGGGGACCTT 
GAGAGCTCCG TCACTGGGTC CATGATCAAT GGCTGGGGCT CAGCCTCGGA AGAGGACAAC 
ATTTCCAGTG GGCGCTCCAG TGTCAGTTCA TCCGATGGCT CCTTTTTCAC TGACGCTGAT 
TTTGCCCAGG CAGTTGCTGC AGCTGCGGAG TATGCGGGCC TGAAAGTGGC TCGCCGCCAA 
ATGCAAGATG CTGCTGGCCG CCGCCACTTC CATGCCTCTC AGTGCCCAAG GCCCACGAGT 
CCTGTGTCCA CAGACAGCAA CATGAGTGCT GTTGTGATCC AGAAAGCCAG ACCCGCCAAG 
AAGCAGAAAC ACCAGCCAGG ACATCTGCGC AGGGAAGCCT ACGCAGATGA TCTTCCACCC 
CCTCCAGTGC CACCACCTGC TATAAAATCG CCCACTGTCC AGTCCAAGGC ACAGCTGGAG 
GTACGGCCTG TCATGGTGCC AAAACTCGCG TCTATAGAAG CAAGGACAGA TAGATCGTCA 
GACAGAAAAG GAGGCAGTTA CAAGGGGAGA GAAGCTCTGG ATGGAAGACA AGTCACTGAC 
CTGCGAACAA ATCCAAGTGA CCCCAGAGAG GCACAGGAAC AGCCAAATGA CGGGAAAGGC 
CGAGGAACAA AGGCAGCCAA GCGAGACCTT CCACCAGCAA AGACGCACCT CGGCCAAGAG 
GATATTCTTC CGTACTGTAG ACCTACTTTC CCAACGTCAA ACAACCCTAG AGACCCCAGT 
TCCTCCAGCT CTATGTCATC AAGAGGATCA GGGAGCCGAC AAAGAGAGCA AGCAAATGTG 
GGCCGAAGGA ATATGGCAGA AATGCAAGTA CTTGGGGGAT TTGAAAGAGG AGATGAGAAT 
AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_030249005.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104865.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X14, mRNA.

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CloneID	OMu86898
Clone ID Related Accession (Same CDS sequence)	XM_030249005.1
Accession Version	XM_030249005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC 
TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA 
GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG 
ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG 
CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC 
AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC 
ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG 
CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT 
ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT 
TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA 
GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG 
GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT 
GAAGCTTCCG CTACTTTGAC AGTTCAAGAG CCGCCACATT TCGTTGTAAA ACCTCGGGAC 
CAGGTGGTTG CCTTGGGAAG AACTGTGACG TTTCAGTGCG AAGCAACTGG AAATCCTCAA 
CCAGCTATCT TTTGGAGGAG AGAGGGGAGT CAGAATCTAC TTTTCTCCTA CCAGCCTCCA 
CAGTCCTCTA GCCGATTTTC AGTTTCGCAG ACAGGAGACC TCACCATTAC GAACGTCCAG 
CGGTCTGATG TCGGCTACTA CATCTGTCAG ACTTTAAATG TTGCTGGAAG TATCATCACA 
AAGGCCTATT TGGAAGTTAC TGATGTGATT GCAGACCGGC CTCCCCCAGT CATTCGGCAA 
GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGCACTTTAA TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG CACCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT 
TCTCGGATCA AACAGCTGGA GAGTGGCGTG CTGCAGATCC GCTACGCTAA GCTGGGTGAC 
ACGGGTCGGT ACACCTGCAC CGCATCGACC CCCAGCGGTG AGGCCACGTG GAGCGCTTAC 
ATCGAAGTCC AGGAATTTGG GGTTCCAGTT CAACCCCCAA GACCCACCGA CCCCAACTTA 
ATCCCCAGCG CCCCCTCAAA GCCCGAAGTT ACAGATGTCA GCAAAAACAC GGTCACTTTA 
TCGTGGCAGC CAAACTTGAA CTCAGGAGCA ACCCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACG GCGGCAGAAA ATGTGAAAAC AGAAACATTT 
GCCATCAAAG GACTCAAACC TAACGCCATT TACCTTTTCC TGGTGAGGGC AGCAAATGCC 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACGCAAGA TGTCCCACCA 
ACCAGCCAAG GGGTGGACCA CAAGCAGGTC CAAAGAGAAC TGGGGAATGT TGTCTTACAT 
CTGCACAACC CGACCATCCT TTCTTCCTCC TCAGTCGAAG TGCACTGGAC GGTGGACCAA 
CAATCTCAAT ATATTCAAGG CTATAAAATT CTCTACCGCC CATCTGGAGC CAGCCATGGT 
GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACGCCAACGA AAAACAGCGT GGTGATCCCC 
GATCTCAGAA AAGGCGTCAA CTACGAGATT AAGGCTCGCC CATTTTTTAA TGAGTTTCAA 
GGAGCAGACA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC 
CGAAGTGTAA CTGTATCCAA GAATGATGGA AATGGGACTG CAATCCTGGT CACTTGGCAA 
CCGCCTCCTG AAGACACACA AAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGT 
AATGAAACGA AGTATCACAT AAACAAGACA GTCGATGGCT CGACCTTCTC GGTGGTCATC 
CCCTCTCTGG TTCCTGGGAT CCGATACAGT GTGGAGGTGG CAGCGAGCAC TGGGGCGGGT 
CCCGGGGTGA AGAGCGAACC TCAGTTCATC CAGTTAGATT CTCACGGAAA CCCCGTGTCT 
CCCGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCCGACG TGGTGAGGCA GCCGGCGTTC 
ATTGCAGGCA TTGGGGCAGC CTGTTGGATT ATCCTCATGG TCTTCAGCAT CTGGCTTTAC 
AGACACCGCA AGAAGAGAAA CGGACTCACC AGCACGTATG CGGGTATCCG GAAAGTAACT 
TATCAAAGAG GAGGCGAAGC CGTCAGCAGT GGAGGGAGGC CGGGACTCCT CAACATTAGT 
GAACCTGCCA CGCAGCCATG GCTGGCAGAC ACGTGGCCCA ACACAGGCAA CAACCACAAT 
GACTGCTCCA TCAACTGCTG CACGGCCGGC AATGGCAACA GCGACAGCAA CCTGACAACC 
TACAGTCGCC CAGCTGATTG TATAGCCAAT TATAACAACC AACTGGATAA CAAACAAACA 
AATCTGATGC TCCCTGAGTC AACTGTTTAT GGTGATGTGG ACCTTAGTAA CAAAATCAAT 
GAGATGAAAA CCTTCAATAG CCCAAATCTG AAGGACGGGC GTTTTGTCAA TCCATCAGGG 
CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT 
AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA 
GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA 
AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG 
TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG 
GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT 
CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA 
AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT 
GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT 
TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG 
GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA 
CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT 
CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA 
GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA 
CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG 
TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC 
AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT 
GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC 
CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC 
AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA 
GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT 
GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG 
CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT 
TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT 
GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC 
AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT 
AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA 
TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA 
GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104865.1
CDS	1366..6177
Translation	MIAEPARFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_030249005.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249005.1

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC 
TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA 
GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG 
ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG 
CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC 
AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC 
ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG 
CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT 
ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT 
TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA 
GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG 
GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT 
GAAGCTTCCG CTACTTTGAC AGTTCAAGAG CCGCCACATT TCGTTGTAAA ACCTCGGGAC 
CAGGTGGTTG CCTTGGGAAG AACTGTGACG TTTCAGTGCG AAGCAACTGG AAATCCTCAA 
CCAGCTATCT TTTGGAGGAG AGAGGGGAGT CAGAATCTAC TTTTCTCCTA CCAGCCTCCA 
CAGTCCTCTA GCCGATTTTC AGTTTCGCAG ACAGGAGACC TCACCATTAC GAACGTCCAG 
CGGTCTGATG TCGGCTACTA CATCTGTCAG ACTTTAAATG TTGCTGGAAG TATCATCACA 
AAGGCCTATT TGGAAGTTAC TGATGTGATT GCAGACCGGC CTCCCCCAGT CATTCGGCAA 
GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGCACTTTAA TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG CACCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT 
TCTCGGATCA AACAGCTGGA GAGTGGCGTG CTGCAGATCC GCTACGCTAA GCTGGGTGAC 
ACGGGTCGGT ACACCTGCAC CGCATCGACC CCCAGCGGTG AGGCCACGTG GAGCGCTTAC 
ATCGAAGTCC AGGAATTTGG GGTTCCAGTT CAACCCCCAA GACCCACCGA CCCCAACTTA 
ATCCCCAGCG CCCCCTCAAA GCCCGAAGTT ACAGATGTCA GCAAAAACAC GGTCACTTTA 
TCGTGGCAGC CAAACTTGAA CTCAGGAGCA ACCCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACG GCGGCAGAAA ATGTGAAAAC AGAAACATTT 
GCCATCAAAG GACTCAAACC TAACGCCATT TACCTTTTCC TGGTGAGGGC AGCAAATGCC 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACGCAAGA TGTCCCACCA 
ACCAGCCAAG GGGTGGACCA CAAGCAGGTC CAAAGAGAAC TGGGGAATGT TGTCTTACAT 
CTGCACAACC CGACCATCCT TTCTTCCTCC TCAGTCGAAG TGCACTGGAC GGTGGACCAA 
CAATCTCAAT ATATTCAAGG CTATAAAATT CTCTACCGCC CATCTGGAGC CAGCCATGGT 
GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACGCCAACGA AAAACAGCGT GGTGATCCCC 
GATCTCAGAA AAGGCGTCAA CTACGAGATT AAGGCTCGCC CATTTTTTAA TGAGTTTCAA 
GGAGCAGACA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC 
CGAAGTGTAA CTGTATCCAA GAATGATGGA AATGGGACTG CAATCCTGGT CACTTGGCAA 
CCGCCTCCTG AAGACACACA AAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGT 
AATGAAACGA AGTATCACAT AAACAAGACA GTCGATGGCT CGACCTTCTC GGTGGTCATC 
CCCTCTCTGG TTCCTGGGAT CCGATACAGT GTGGAGGTGG CAGCGAGCAC TGGGGCGGGT 
CCCGGGGTGA AGAGCGAACC TCAGTTCATC CAGTTAGATT CTCACGGAAA CCCCGTGTCT 
CCCGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCCGACG TGGTGAGGCA GCCGGCGTTC 
ATTGCAGGCA TTGGGGCAGC CTGTTGGATT ATCCTCATGG TCTTCAGCAT CTGGCTTTAC 
AGACACCGCA AGAAGAGAAA CGGACTCACC AGCACGTATG CGGGTATCCG GAAAGTAACT 
TATCAAAGAG GAGGCGAAGC CGTCAGCAGT GGAGGGAGGC CGGGACTCCT CAACATTAGT 
GAACCTGCCA CGCAGCCATG GCTGGCAGAC ACGTGGCCCA ACACAGGCAA CAACCACAAT 
GACTGCTCCA TCAACTGCTG CACGGCCGGC AATGGCAACA GCGACAGCAA CCTGACAACC 
TACAGTCGCC CAGCTGATTG TATAGCCAAT TATAACAACC AACTGGATAA CAAACAAACA 
AATCTGATGC TCCCTGAGTC AACTGTTTAT GGTGATGTGG ACCTTAGTAA CAAAATCAAT 
GAGATGAAAA CCTTCAATAG CCCAAATCTG AAGGACGGGC GTTTTGTCAA TCCATCAGGG 
CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT 
AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA 
GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA 
AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG 
TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG 
GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT 
CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA 
AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT 
GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT 
TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG 
GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA 
CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT 
CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA 
GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA 
CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG 
TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC 
AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT 
GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC 
CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC 
AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA 
GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT 
GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG 
CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT 
TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT 
GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC 
AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT 
AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA 
TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA 
GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( NM_019413.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062286.2 Mus musculus roundabout guidance receptor 1 (Robo1), mRNA.

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CloneID	OMu22089
Clone ID Related Accession (Same CDS sequence)	NM_019413.2
Accession Version	NM_019413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143001.1, Y17793.1, AK040651.1, CK781477.1, AK135605.1 and AC164551.2. On Jan 13, 2007 this sequence version replaced NM_019413.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y17793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC 
TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA 
GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG 
ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG 
CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC 
AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC 
ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG 
CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT 
ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT 
TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA 
GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG 
GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT 
GAAGCTTCCG CTACTTTGAC AGTTCAAGAG CCGCCACATT TCGTTGTAAA ACCTCGGGAC 
CAGGTGGTTG CCTTGGGAAG AACTGTGACG TTTCAGTGCG AAGCAACTGG AAATCCTCAA 
CCAGCTATCT TTTGGAGGAG AGAGGGGAGT CAGAATCTAC TTTTCTCCTA CCAGCCTCCA 
CAGTCCTCTA GCCGATTTTC AGTTTCGCAG ACAGGAGACC TCACCATTAC GAACGTCCAG 
CGGTCTGATG TCGGCTACTA CATCTGTCAG ACTTTAAATG TTGCTGGAAG TATCATCACA 
AAGGCCTATT TGGAAGTTAC TGATGTGATT GCAGACCGGC CTCCCCCAGT CATTCGGCAA 
GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGCACTTTAA TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG CACCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT 
TCTCGGATCA AACAGCTGGA GAGTGGCGTG CTGCAGATCC GCTACGCTAA GCTGGGTGAC 
ACGGGTCGGT ACACCTGCAC CGCATCGACC CCCAGCGGTG AGGCCACGTG GAGCGCTTAC 
ATCGAAGTCC AGGAATTTGG GGTTCCAGTT CAACCCCCAA GACCCACCGA CCCCAACTTA 
ATCCCCAGCG CCCCCTCAAA GCCCGAAGTT ACAGATGTCA GCAAAAACAC GGTCACTTTA 
TCGTGGCAGC CAAACTTGAA CTCAGGAGCA ACCCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACG GCGGCAGAAA ATGTGAAAAC AGAAACATTT 
GCCATCAAAG GACTCAAACC TAACGCCATT TACCTTTTCC TGGTGAGGGC AGCAAATGCC 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACGCAAGA TGTCCCACCA 
ACCAGCCAAG GGGTGGACCA CAAGCAGGTC CAAAGAGAAC TGGGGAATGT TGTCTTACAT 
CTGCACAACC CGACCATCCT TTCTTCCTCC TCAGTCGAAG TGCACTGGAC GGTGGACCAA 
CAATCTCAAT ATATTCAAGG CTATAAAATT CTCTACCGCC CATCTGGAGC CAGCCATGGT 
GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACGCCAACGA AAAACAGCGT GGTGATCCCC 
GATCTCAGAA AAGGCGTCAA CTACGAGATT AAGGCTCGCC CATTTTTTAA TGAGTTTCAA 
GGAGCAGACA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC 
CGAAGTGTAA CTGTATCCAA GAATGATGGA AATGGGACTG CAATCCTGGT CACTTGGCAA 
CCGCCTCCTG AAGACACACA AAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGT 
AATGAAACGA AGTATCACAT AAACAAGACA GTCGATGGCT CGACCTTCTC GGTGGTCATC 
CCCTCTCTGG TTCCTGGGAT CCGATACAGT GTGGAGGTGG CAGCGAGCAC TGGGGCGGGT 
CCCGGGGTGA AGAGCGAACC TCAGTTCATC CAGTTAGATT CTCACGGAAA CCCCGTGTCT 
CCCGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCCGACG TGGTGAGGCA GCCGGCGTTC 
ATTGCAGGCA TTGGGGCAGC CTGTTGGATT ATCCTCATGG TCTTCAGCAT CTGGCTTTAC 
AGACACCGCA AGAAGAGAAA CGGACTCACC AGCACGTATG CGGGTATCCG GAAAGTCCCG 
TCCTTTACCT TCACGCCAAC AGTAACTTAT CAAAGAGGAG GCGAAGCCGT CAGCAGTGGA 
GGGAGGCCGG GACTCCTCAA CATTAGTGAA CCTGCCACGC AGCCATGGCT GGCAGACACG 
TGGCCCAACA CAGGCAACAA CCACAATGAC TGCTCCATCA ACTGCTGCAC GGCCGGCAAT 
GGCAACAGCG ACAGCAACCT GACAACCTAC AGTCGCCCAG CTGATTGTAT AGCCAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GACGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC 
CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG 
AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC 
CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA 
TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC 
ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT 
GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA 
CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC 
ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC 
ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG 
GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT 
CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC 
TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC 
AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT 
GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA 
GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT 
GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT 
CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG 
CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA 
CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT 
CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA 
CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT 
AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA 
GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC 
GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC 
GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA 
GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA 
GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA 
GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062286.2
CDS	992..5830
Translation	MIAEPARFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	NM_019413.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019413.2

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC 
TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA 
GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG 
ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG 
CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC 
AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC 
ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG 
CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT 
ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT 
TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA 
GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG 
GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT 
GAAGCTTCCG CTACTTTGAC AGTTCAAGAG CCGCCACATT TCGTTGTAAA ACCTCGGGAC 
CAGGTGGTTG CCTTGGGAAG AACTGTGACG TTTCAGTGCG AAGCAACTGG AAATCCTCAA 
CCAGCTATCT TTTGGAGGAG AGAGGGGAGT CAGAATCTAC TTTTCTCCTA CCAGCCTCCA 
CAGTCCTCTA GCCGATTTTC AGTTTCGCAG ACAGGAGACC TCACCATTAC GAACGTCCAG 
CGGTCTGATG TCGGCTACTA CATCTGTCAG ACTTTAAATG TTGCTGGAAG TATCATCACA 
AAGGCCTATT TGGAAGTTAC TGATGTGATT GCAGACCGGC CTCCCCCAGT CATTCGGCAA 
GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGCACTTTAA TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG CACCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT 
TCTCGGATCA AACAGCTGGA GAGTGGCGTG CTGCAGATCC GCTACGCTAA GCTGGGTGAC 
ACGGGTCGGT ACACCTGCAC CGCATCGACC CCCAGCGGTG AGGCCACGTG GAGCGCTTAC 
ATCGAAGTCC AGGAATTTGG GGTTCCAGTT CAACCCCCAA GACCCACCGA CCCCAACTTA 
ATCCCCAGCG CCCCCTCAAA GCCCGAAGTT ACAGATGTCA GCAAAAACAC GGTCACTTTA 
TCGTGGCAGC CAAACTTGAA CTCAGGAGCA ACCCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACG GCGGCAGAAA ATGTGAAAAC AGAAACATTT 
GCCATCAAAG GACTCAAACC TAACGCCATT TACCTTTTCC TGGTGAGGGC AGCAAATGCC 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACGCAAGA TGTCCCACCA 
ACCAGCCAAG GGGTGGACCA CAAGCAGGTC CAAAGAGAAC TGGGGAATGT TGTCTTACAT 
CTGCACAACC CGACCATCCT TTCTTCCTCC TCAGTCGAAG TGCACTGGAC GGTGGACCAA 
CAATCTCAAT ATATTCAAGG CTATAAAATT CTCTACCGCC CATCTGGAGC CAGCCATGGT 
GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACGCCAACGA AAAACAGCGT GGTGATCCCC 
GATCTCAGAA AAGGCGTCAA CTACGAGATT AAGGCTCGCC CATTTTTTAA TGAGTTTCAA 
GGAGCAGACA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC 
CGAAGTGTAA CTGTATCCAA GAATGATGGA AATGGGACTG CAATCCTGGT CACTTGGCAA 
CCGCCTCCTG AAGACACACA AAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGT 
AATGAAACGA AGTATCACAT AAACAAGACA GTCGATGGCT CGACCTTCTC GGTGGTCATC 
CCCTCTCTGG TTCCTGGGAT CCGATACAGT GTGGAGGTGG CAGCGAGCAC TGGGGCGGGT 
CCCGGGGTGA AGAGCGAACC TCAGTTCATC CAGTTAGATT CTCACGGAAA CCCCGTGTCT 
CCCGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCCGACG TGGTGAGGCA GCCGGCGTTC 
ATTGCAGGCA TTGGGGCAGC CTGTTGGATT ATCCTCATGG TCTTCAGCAT CTGGCTTTAC 
AGACACCGCA AGAAGAGAAA CGGACTCACC AGCACGTATG CGGGTATCCG GAAAGTCCCG 
TCCTTTACCT TCACGCCAAC AGTAACTTAT CAAAGAGGAG GCGAAGCCGT CAGCAGTGGA 
GGGAGGCCGG GACTCCTCAA CATTAGTGAA CCTGCCACGC AGCCATGGCT GGCAGACACG 
TGGCCCAACA CAGGCAACAA CCACAATGAC TGCTCCATCA ACTGCTGCAC GGCCGGCAAT 
GGCAACAGCG ACAGCAACCT GACAACCTAC AGTCGCCCAG CTGATTGTAT AGCCAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GACGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC 
CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG 
AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC 
CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA 
TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC 
ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT 
GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA 
CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC 
ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC 
ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG 
GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT 
CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC 
TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC 
AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT 
GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA 
GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT 
GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT 
CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG 
CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA 
CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT 
CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA 
CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT 
AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA 
GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC 
GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC 
GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA 
GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA 
GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA 
GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522957.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523020.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X2, mRNA.

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CloneID	OMu52697
Clone ID Related Accession (Same CDS sequence)	XM_006522957.2
Accession Version	XM_006522957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006522957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGAGCCGC CACATTTCGT TGTAAAACCT CGGGACCAGG TGGTTGCCTT GGGAAGAACT 
GTGACGTTTC AGTGCGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GAGGAGAGAG 
GGGAGTCAGA ATCTACTTTT CTCCTACCAG CCTCCACAGT CCTCTAGCCG ATTTTCAGTT 
TCGCAGACAG GAGACCTCAC CATTACGAAC GTCCAGCGGT CTGATGTCGG CTACTACATC 
TGTCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAGG CCTATTTGGA AGTTACTGAT 
GTGATTGCAG ACCGGCCTCC CCCAGTCATT CGGCAAGGTC CTGTGAATCA GACTGTAGCA 
GTGGATGGCA CTTTAATCCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACAATTCTG 
TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGT 
GGCGTGCTGC AGATCCGCTA CGCTAAGCTG GGTGACACGG GTCGGTACAC CTGCACCGCA 
TCGACCCCCA GCGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGGGTT 
CCAGTTCAAC CCCCAAGACC CACCGACCCC AACTTAATCC CCAGCGCCCC CTCAAAGCCC 
GAAGTTACAG ATGTCAGCAA AAACACGGTC ACTTTATCGT GGCAGCCAAA CTTGAACTCA 
GGAGCAACCC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG 
CAGACGGCGG CAGAAAATGT GAAAACAGAA ACATTTGCCA TCAAAGGACT CAAACCTAAC 
GCCATTTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GAATTAGTGA TCCAAGCCAA 
ATATCAGATC CAGTGAAAAC GCAAGATGTC CCACCAACCA GCCAAGGGGT GGACCACAAG 
CAGGTCCAAA GAGAACTGGG GAATGTTGTC TTACATCTGC ACAACCCGAC CATCCTTTCT 
TCCTCCTCAG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGCTAT 
AAAATTCTCT ACCGCCCATC TGGAGCCAGC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA 
GTGAGGACGC CAACGAAAAA CAGCGTGGTG ATCCCCGATC TCAGAAAAGG CGTCAACTAC 
GAGATTAAGG CTCGCCCATT TTTTAATGAG TTTCAAGGAG CAGACAGTGA AATCAAATTT 
GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTAACTGT ATCCAAGAAT 
GATGGAAATG GGACTGCAAT CCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAAAAT 
GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGTAATG AAACGAAGTA TCACATAAAC 
AAGACAGTCG ATGGCTCGAC CTTCTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGA 
TACAGTGTGG AGGTGGCAGC GAGCACTGGG GCGGGTCCCG GGGTGAAGAG CGAACCTCAG 
TTCATCCAGT TAGATTCTCA CGGAAACCCC GTGTCTCCCG AGGACCAAGT CAGCCTTGCT 
CAGCAGATCT CCGACGTGGT GAGGCAGCCG GCGTTCATTG CAGGCATTGG GGCAGCCTGT 
TGGATTATCC TCATGGTCTT CAGCATCTGG CTTTACAGAC ACCGCAAGAA GAGAAACGGA 
CTCACCAGCA CGTATGCGGG TATCCGGAAA GTCCCGTCCT TTACCTTCAC GCCAACAGTA 
ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATT 
AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CCAACACAGG CAACAACCAC 
AATGACTGCT CCATCAACTG CTGCACGGCC GGCAATGGCA ACAGCGACAG CAACCTGACA 
ACCTACAGTC GCCCAGCTGA TTGTATAGCC AATTATAACA ACCAACTGGA TAACAAACAA 
ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC 
AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGACG GGCGTTTTGT CAATCCATCA 
GGGCAGCCCA CTCCCTATGC CACCACTCAA CTCATCCAGG CGAACCTCAG CAACAATATG 
AATAATGGCG CCGGGGACTC CAGCGAGAAG CACTGGAAGC CTCCGGGACA GCAGAAACCG 
GAAGTGGCAC CAATTCAGTA TAACATCATG GAGCAAAACA AGCTGAACAA AGATTATCGG 
GCAAATGACA CGATCCCTCC AACCATCCCA TACAACCAGT CCTATGACCA GAACACAGGA 
GGGTCTTATA ACAGCTCAGA TCGGGGCAGT AGTACATCCG GGAGTCAAGG CCACAAGAAA 
GGGGCACGGA CACCAAAGGC ACCCAAGCAG GGTGGCATGA ACTGGGCAGA CTTGCTGCCC 
CCTCCTCCGG CGCATCCTCC CCCACATAGC AACAGTGAAG AATACAACAT GTCCGTAGAT 
GAAAGCTATG ATCAAGAAAT GCCCTGTCCA GTGCCACCTG CGCGGATGTA TTTGCAACAG 
GATGAATTAG AGGAAGAGGA AGATGAAAGA GGCCCCACTC CCCCTGTCCG GGGGGCAGCT 
TCTTCTCCAG CTGCTGTGTC CTACAGCCAC CAATCCACGG CCACTCTCAC CCCTTCTCCC 
CAGGAAGAGC TCCAGCCCAT GTTACAGGAT TGTCCGGAGG ACCTCGGGCA CATGCCCCAC 
CCACCAGACA GGAGGCGGCA GCCCGTGAGT CCTCCTCCAC CGCCACGGCC AATTTCCCCA 
CCTCATACTT ACGGCTACAT TTCAGGACCC CTTGTCTCAG ATATGGATAC CGATGCCCCA 
GAAGAGGAGG AGGACGAAGC TGACATGGAA GTTGCCAAAA TGCAGACCCG AAGGCTGTTG 
TTACGTGGGC TGGAGCAGAC CCCAGCATCC AGTGTTGGGG ACCTTGAGAG CTCCGTCACT 
GGGTCCATGA TCAATGGCTG GGGCTCAGCC TCGGAAGAGG ACAACATTTC CAGTGGGCGC 
TCCAGTGTCA GTTCATCCGA TGGCTCCTTT TTCACTGACG CTGATTTTGC CCAGGCAGTT 
GCTGCAGCTG CGGAGTATGC GGGCCTGAAA GTGGCTCGCC GCCAAATGCA AGATGCTGCT 
GGCCGCCGCC ACTTCCATGC CTCTCAGTGC CCAAGGCCCA CGAGTCCTGT GTCCACAGAC 
AGCAACATGA GTGCTGTTGT GATCCAGAAA GCCAGACCCG CCAAGAAGCA GAAACACCAG 
CCAGGACATC TGCGCAGGGA AGCCTACGCA GATGATCTTC CACCCCCTCC AGTGCCACCA 
CCTGCTATAA AATCGCCCAC TGTCCAGTCC AAGGCACAGC TGGAGGTACG GCCTGTCATG 
GTGCCAAAAC TCGCGTCTAT AGAAGCAAGG ACAGATAGAT CGTCAGACAG AAAAGGAGGC 
AGTTACAAGG GGAGAGAAGC TCTGGATGGA AGACAAGTCA CTGACCTGCG AACAAATCCA 
AGTGACCCCA GAGAGGCACA GGAACAGCCA AATGACGGGA AAGGCCGAGG AACAAAGGCA 
GCCAAGCGAG ACCTTCCACC AGCAAAGACG CACCTCGGCC AAGAGGATAT TCTTCCGTAC 
TGTAGACCTA CTTTCCCAAC GTCAAACAAC CCTAGAGACC CCAGTTCCTC CAGCTCTATG 
TCATCAAGAG GATCAGGGAG CCGACAAAGA GAGCAAGCAA ATGTGGGCCG AAGGAATATG 
GCAGAAATGC AAGTACTTGG GGGATTTGAA AGAGGAGATG AGAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523020.1
CDS	98..5152
Translation	MVLRPARGSVCCRKGKEDVCCVLSLCVFLSHVDLLVSGHAVIEMATTVTSVTPSAALVLFPTDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522957.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522957.2

ORF Insert Sequence:

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGAGCCGC CACATTTCGT TGTAAAACCT CGGGACCAGG TGGTTGCCTT GGGAAGAACT 
GTGACGTTTC AGTGCGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GAGGAGAGAG 
GGGAGTCAGA ATCTACTTTT CTCCTACCAG CCTCCACAGT CCTCTAGCCG ATTTTCAGTT 
TCGCAGACAG GAGACCTCAC CATTACGAAC GTCCAGCGGT CTGATGTCGG CTACTACATC 
TGTCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAGG CCTATTTGGA AGTTACTGAT 
GTGATTGCAG ACCGGCCTCC CCCAGTCATT CGGCAAGGTC CTGTGAATCA GACTGTAGCA 
GTGGATGGCA CTTTAATCCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACAATTCTG 
TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGT 
GGCGTGCTGC AGATCCGCTA CGCTAAGCTG GGTGACACGG GTCGGTACAC CTGCACCGCA 
TCGACCCCCA GCGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGGGTT 
CCAGTTCAAC CCCCAAGACC CACCGACCCC AACTTAATCC CCAGCGCCCC CTCAAAGCCC 
GAAGTTACAG ATGTCAGCAA AAACACGGTC ACTTTATCGT GGCAGCCAAA CTTGAACTCA 
GGAGCAACCC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG 
CAGACGGCGG CAGAAAATGT GAAAACAGAA ACATTTGCCA TCAAAGGACT CAAACCTAAC 
GCCATTTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GAATTAGTGA TCCAAGCCAA 
ATATCAGATC CAGTGAAAAC GCAAGATGTC CCACCAACCA GCCAAGGGGT GGACCACAAG 
CAGGTCCAAA GAGAACTGGG GAATGTTGTC TTACATCTGC ACAACCCGAC CATCCTTTCT 
TCCTCCTCAG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGCTAT 
AAAATTCTCT ACCGCCCATC TGGAGCCAGC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA 
GTGAGGACGC CAACGAAAAA CAGCGTGGTG ATCCCCGATC TCAGAAAAGG CGTCAACTAC 
GAGATTAAGG CTCGCCCATT TTTTAATGAG TTTCAAGGAG CAGACAGTGA AATCAAATTT 
GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTAACTGT ATCCAAGAAT 
GATGGAAATG GGACTGCAAT CCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAAAAT 
GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGTAATG AAACGAAGTA TCACATAAAC 
AAGACAGTCG ATGGCTCGAC CTTCTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGA 
TACAGTGTGG AGGTGGCAGC GAGCACTGGG GCGGGTCCCG GGGTGAAGAG CGAACCTCAG 
TTCATCCAGT TAGATTCTCA CGGAAACCCC GTGTCTCCCG AGGACCAAGT CAGCCTTGCT 
CAGCAGATCT CCGACGTGGT GAGGCAGCCG GCGTTCATTG CAGGCATTGG GGCAGCCTGT 
TGGATTATCC TCATGGTCTT CAGCATCTGG CTTTACAGAC ACCGCAAGAA GAGAAACGGA 
CTCACCAGCA CGTATGCGGG TATCCGGAAA GTCCCGTCCT TTACCTTCAC GCCAACAGTA 
ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATT 
AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CCAACACAGG CAACAACCAC 
AATGACTGCT CCATCAACTG CTGCACGGCC GGCAATGGCA ACAGCGACAG CAACCTGACA 
ACCTACAGTC GCCCAGCTGA TTGTATAGCC AATTATAACA ACCAACTGGA TAACAAACAA 
ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC 
AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGACG GGCGTTTTGT CAATCCATCA 
GGGCAGCCCA CTCCCTATGC CACCACTCAA CTCATCCAGG CGAACCTCAG CAACAATATG 
AATAATGGCG CCGGGGACTC CAGCGAGAAG CACTGGAAGC CTCCGGGACA GCAGAAACCG 
GAAGTGGCAC CAATTCAGTA TAACATCATG GAGCAAAACA AGCTGAACAA AGATTATCGG 
GCAAATGACA CGATCCCTCC AACCATCCCA TACAACCAGT CCTATGACCA GAACACAGGA 
GGGTCTTATA ACAGCTCAGA TCGGGGCAGT AGTACATCCG GGAGTCAAGG CCACAAGAAA 
GGGGCACGGA CACCAAAGGC ACCCAAGCAG GGTGGCATGA ACTGGGCAGA CTTGCTGCCC 
CCTCCTCCGG CGCATCCTCC CCCACATAGC AACAGTGAAG AATACAACAT GTCCGTAGAT 
GAAAGCTATG ATCAAGAAAT GCCCTGTCCA GTGCCACCTG CGCGGATGTA TTTGCAACAG 
GATGAATTAG AGGAAGAGGA AGATGAAAGA GGCCCCACTC CCCCTGTCCG GGGGGCAGCT 
TCTTCTCCAG CTGCTGTGTC CTACAGCCAC CAATCCACGG CCACTCTCAC CCCTTCTCCC 
CAGGAAGAGC TCCAGCCCAT GTTACAGGAT TGTCCGGAGG ACCTCGGGCA CATGCCCCAC 
CCACCAGACA GGAGGCGGCA GCCCGTGAGT CCTCCTCCAC CGCCACGGCC AATTTCCCCA 
CCTCATACTT ACGGCTACAT TTCAGGACCC CTTGTCTCAG ATATGGATAC CGATGCCCCA 
GAAGAGGAGG AGGACGAAGC TGACATGGAA GTTGCCAAAA TGCAGACCCG AAGGCTGTTG 
TTACGTGGGC TGGAGCAGAC CCCAGCATCC AGTGTTGGGG ACCTTGAGAG CTCCGTCACT 
GGGTCCATGA TCAATGGCTG GGGCTCAGCC TCGGAAGAGG ACAACATTTC CAGTGGGCGC 
TCCAGTGTCA GTTCATCCGA TGGCTCCTTT TTCACTGACG CTGATTTTGC CCAGGCAGTT 
GCTGCAGCTG CGGAGTATGC GGGCCTGAAA GTGGCTCGCC GCCAAATGCA AGATGCTGCT 
GGCCGCCGCC ACTTCCATGC CTCTCAGTGC CCAAGGCCCA CGAGTCCTGT GTCCACAGAC 
AGCAACATGA GTGCTGTTGT GATCCAGAAA GCCAGACCCG CCAAGAAGCA GAAACACCAG 
CCAGGACATC TGCGCAGGGA AGCCTACGCA GATGATCTTC CACCCCCTCC AGTGCCACCA 
CCTGCTATAA AATCGCCCAC TGTCCAGTCC AAGGCACAGC TGGAGGTACG GCCTGTCATG 
GTGCCAAAAC TCGCGTCTAT AGAAGCAAGG ACAGATAGAT CGTCAGACAG AAAAGGAGGC 
AGTTACAAGG GGAGAGAAGC TCTGGATGGA AGACAAGTCA CTGACCTGCG AACAAATCCA 
AGTGACCCCA GAGAGGCACA GGAACAGCCA AATGACGGGA AAGGCCGAGG AACAAAGGCA 
GCCAAGCGAG ACCTTCCACC AGCAAAGACG CACCTCGGCC AAGAGGATAT TCTTCCGTAC 
TGTAGACCTA CTTTCCCAAC GTCAAACAAC CCTAGAGACC CCAGTTCCTC CAGCTCTATG 
TCATCAAGAG GATCAGGGAG CCGACAAAGA GAGCAAGCAA ATGTGGGCCG AAGGAATATG 
GCAGAAATGC AAGTACTTGG GGGATTTGAA AGAGGAGATG AGAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522958.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523021.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu52698
Clone ID Related Accession (Same CDS sequence)	XM_006522958.2
Accession Version	XM_006522958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006522958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT 
GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT 
GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC 
TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG 
TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT 
CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA 
GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG 
GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT 
CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA 
ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG 
GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG 
TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC 
ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG 
CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC 
ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA 
GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG 
ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC 
TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC 
GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT 
GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT 
GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT 
GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG 
GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC 
ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT 
ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA 
GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA 
CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA 
CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA 
ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG 
AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT 
GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523021.1
CDS	99..5135
Translation	MVLRPARGSVCCRKGKEDVCCVLSLCVFLSHVDLLVSGHAVIEMATTVTSVTPSAALVLFPTDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522958.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522958.2

ORF Insert Sequence:

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT 
GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT 
GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC 
TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG 
TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT 
CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA 
GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG 
GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT 
CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA 
ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG 
GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG 
TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC 
ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG 
CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC 
ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA 
GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG 
ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC 
TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC 
GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT 
GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT 
GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT 
GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG 
GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC 
ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT 
ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA 
GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA 
CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA 
CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA 
ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG 
AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT 
GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522960.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523023.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu52700
Clone ID Related Accession (Same CDS sequence)	XM_006522960.3 , XM_006522960.4
Accession Version	XM_006522960.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG ACCCTGAGGA CCTAGAGAGG 
GGGAACGACA ACGGAACGCC AGCCCCCACC TCTGATAACG ATGACAATTC GCTGGGCTAC 
ACAGGCTCCC GTCTTCGTCA GGAAGATTTT CCACCCCGCA TTGTTGAACA CCCTTCAGAC 
CTGATCGTCT CCAAAGGAGA ACCTGCCACT CTGAACTGTA AAGCCGAAGG CCGCCCCACC 
CCGACGATAG AATGGTACAA AGGTGGGGAA AGAGTGGAGA CGGACAAAGA TGACCCGCGC 
TCACACCGGA TGCTGCTGCC AAGCGGGTCT TTATTTTTCT TACGCATAGT CCATGGACGG 
AAGAGTAGGC CCGACGAAGG GGTCTACATC TGTGTGGCTA GGAATTACCT CGGAGAGGCT 
GTGAGCCACA ACGCATCGCT GGAGGTAGCT ATACTACGGG ATGACTTCAG ACAAAACCCT 
TCAGATGTCA TGGTGGCAGT AGGCGAACCT GCAGTCATGG AATGCCAGCC TCCACGGGGC 
CATCCAGAGC CCACCATCTC CTGGAAAAAG GATGGCTCAC CCCTGGATGA TAAAGATGAA 
AGAATCACTA TACGAGGAGG AAAACTCATG ATCACTTATA CGCGTAAAAG CGATGCTGGC 
AAGTATGTTT GCGTTGGCAC CAACATGGTG GGGGAACGGG AGAGTGAAGT CGCAGAGCTA 
ACCGTTTTAG AGAGACCATC GTTTGTGAAG AGACCCAGTA ATTTGGCAGT GACTGTGGAT 
GACAGTGCAG AATTTAAATG TGAGGCCCGA GGAGACCCTG TGCCTACAGT TCGATGGAGG 
AAAGATGACG GAGAGCTGCC CAAATCCAGA TATGAAATCC GAGATGATCA TACTTTGAAA 
ATTCGGAAGG TGACTGCTGG CGACATGGGA TCATACACTT GTGTGGCAGA AAATATGGTC 
GGGAAAGCTG AAGCTTCCGC TACTTTGACA GTTCAAGTTG GGTCTGAGCC GCCACATTTC 
GTTGTAAAAC CTCGGGACCA GGTGGTTGCC TTGGGAAGAA CTGTGACGTT TCAGTGCGAA 
GCAACTGGAA ATCCTCAACC AGCTATCTTT TGGAGGAGAG AGGGGAGTCA GAATCTACTT 
TTCTCCTACC AGCCTCCACA GTCCTCTAGC CGATTTTCAG TTTCGCAGAC AGGAGACCTC 
ACCATTACGA ACGTCCAGCG GTCTGATGTC GGCTACTACA TCTGTCAGAC TTTAAATGTT 
GCTGGAAGTA TCATCACAAA GGCCTATTTG GAAGTTACTG ATGTGATTGC AGACCGGCCT 
CCCCCAGTCA TTCGGCAAGG TCCTGTGAAT CAGACTGTAG CAGTGGATGG CACTTTAATC 
CTCAGCTGTG TGGCCACAGG CAGTCCAGCA CCCACAATTC TGTGGAGGAA GGATGGAGTC 
CTTGTTTCAA CCCAAGATTC TCGGATCAAA CAGCTGGAGA GTGGCGTGCT GCAGATCCGC 
TACGCTAAGC TGGGTGACAC GGGTCGGTAC ACCTGCACCG CATCGACCCC CAGCGGTGAG 
GCCACGTGGA GCGCTTACAT CGAAGTCCAG GAATTTGGGG TTCCAGTTCA ACCCCCAAGA 
CCCACCGACC CCAACTTAAT CCCCAGCGCC CCCTCAAAGC CCGAAGTTAC AGATGTCAGC 
AAAAACACGG TCACTTTATC GTGGCAGCCA AACTTGAACT CAGGAGCAAC CCCAACATCT 
TATATTATAG AAGCCTTCAG CCATGCATCT GGTAGCAGCT GGCAGACGGC GGCAGAAAAT 
GTGAAAACAG AAACATTTGC CATCAAAGGA CTCAAACCTA ACGCCATTTA CCTTTTCCTG 
GTGAGGGCAG CAAATGCCTA TGGAATTAGT GATCCAAGCC AAATATCAGA TCCAGTGAAA 
ACGCAAGATG TCCCACCAAC CAGCCAAGGG GTGGACCACA AGCAGGTCCA AAGAGAACTG 
GGGAATGTTG TCTTACATCT GCACAACCCG ACCATCCTTT CTTCCTCCTC AGTCGAAGTG 
CACTGGACGG TGGACCAACA ATCTCAATAT ATTCAAGGCT ATAAAATTCT CTACCGCCCA 
TCTGGAGCCA GCCATGGTGA ATCGGAGTGG TTAGTTTTTG AAGTGAGGAC GCCAACGAAA 
AACAGCGTGG TGATCCCCGA TCTCAGAAAA GGCGTCAACT ACGAGATTAA GGCTCGCCCA 
TTTTTTAATG AGTTTCAAGG AGCAGACAGT GAAATCAAAT TTGCCAAAAC CTTAGAAGAA 
GCACCAAGTG CCCCTCCCCG AAGTGTAACT GTATCCAAGA ATGATGGAAA TGGGACTGCA 
ATCCTGGTCA CTTGGCAACC GCCTCCTGAA GACACACAAA ATGGAATGGT CCAAGAATAT 
AAGGTTTGGT GTCTCGGTAA TGAAACGAAG TATCACATAA ACAAGACAGT CGATGGCTCG 
ACCTTCTCGG TGGTCATCCC CTCTCTGGTT CCTGGGATCC GATACAGTGT GGAGGTGGCA 
GCGAGCACTG GGGCGGGTCC CGGGGTGAAG AGCGAACCTC AGTTCATCCA GTTAGATTCT 
CACGGAAACC CCGTGTCTCC CGAGGACCAA GTCAGCCTTG CTCAGCAGAT CTCCGACGTG 
GTGAGGCAGC CGGCGTTCAT TGCAGGCATT GGGGCAGCCT GTTGGATTAT CCTCATGGTC 
TTCAGCATCT GGCTTTACAG ACACCGCAAG AAGAGAAACG GACTCACCAG CACGTATGCG 
GGTATCCGGA AAGTCCCGTC CTTTACCTTC ACGCCAACAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT 
GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT 
GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC 
TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG 
TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT 
CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA 
GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG 
GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT 
CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA 
ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG 
GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG 
TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC 
ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG 
CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC 
ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA 
GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG 
ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC 
TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC 
GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT 
GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT 
GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT 
GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG 
GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC 
ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT 
ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA 
GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA 
CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA 
CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA 
ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG 
AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT 
GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523023.1
CDS	393..5369
Translation	MGGIKMKWKHLPLLVISLLTLSRKQLLLAQLIPDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522960.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522960.3

ORF Insert Sequence:

ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG ACCCTGAGGA CCTAGAGAGG 
GGGAACGACA ACGGAACGCC AGCCCCCACC TCTGATAACG ATGACAATTC GCTGGGCTAC 
ACAGGCTCCC GTCTTCGTCA GGAAGATTTT CCACCCCGCA TTGTTGAACA CCCTTCAGAC 
CTGATCGTCT CCAAAGGAGA ACCTGCCACT CTGAACTGTA AAGCCGAAGG CCGCCCCACC 
CCGACGATAG AATGGTACAA AGGTGGGGAA AGAGTGGAGA CGGACAAAGA TGACCCGCGC 
TCACACCGGA TGCTGCTGCC AAGCGGGTCT TTATTTTTCT TACGCATAGT CCATGGACGG 
AAGAGTAGGC CCGACGAAGG GGTCTACATC TGTGTGGCTA GGAATTACCT CGGAGAGGCT 
GTGAGCCACA ACGCATCGCT GGAGGTAGCT ATACTACGGG ATGACTTCAG ACAAAACCCT 
TCAGATGTCA TGGTGGCAGT AGGCGAACCT GCAGTCATGG AATGCCAGCC TCCACGGGGC 
CATCCAGAGC CCACCATCTC CTGGAAAAAG GATGGCTCAC CCCTGGATGA TAAAGATGAA 
AGAATCACTA TACGAGGAGG AAAACTCATG ATCACTTATA CGCGTAAAAG CGATGCTGGC 
AAGTATGTTT GCGTTGGCAC CAACATGGTG GGGGAACGGG AGAGTGAAGT CGCAGAGCTA 
ACCGTTTTAG AGAGACCATC GTTTGTGAAG AGACCCAGTA ATTTGGCAGT GACTGTGGAT 
GACAGTGCAG AATTTAAATG TGAGGCCCGA GGAGACCCTG TGCCTACAGT TCGATGGAGG 
AAAGATGACG GAGAGCTGCC CAAATCCAGA TATGAAATCC GAGATGATCA TACTTTGAAA 
ATTCGGAAGG TGACTGCTGG CGACATGGGA TCATACACTT GTGTGGCAGA AAATATGGTC 
GGGAAAGCTG AAGCTTCCGC TACTTTGACA GTTCAAGTTG GGTCTGAGCC GCCACATTTC 
GTTGTAAAAC CTCGGGACCA GGTGGTTGCC TTGGGAAGAA CTGTGACGTT TCAGTGCGAA 
GCAACTGGAA ATCCTCAACC AGCTATCTTT TGGAGGAGAG AGGGGAGTCA GAATCTACTT 
TTCTCCTACC AGCCTCCACA GTCCTCTAGC CGATTTTCAG TTTCGCAGAC AGGAGACCTC 
ACCATTACGA ACGTCCAGCG GTCTGATGTC GGCTACTACA TCTGTCAGAC TTTAAATGTT 
GCTGGAAGTA TCATCACAAA GGCCTATTTG GAAGTTACTG ATGTGATTGC AGACCGGCCT 
CCCCCAGTCA TTCGGCAAGG TCCTGTGAAT CAGACTGTAG CAGTGGATGG CACTTTAATC 
CTCAGCTGTG TGGCCACAGG CAGTCCAGCA CCCACAATTC TGTGGAGGAA GGATGGAGTC 
CTTGTTTCAA CCCAAGATTC TCGGATCAAA CAGCTGGAGA GTGGCGTGCT GCAGATCCGC 
TACGCTAAGC TGGGTGACAC GGGTCGGTAC ACCTGCACCG CATCGACCCC CAGCGGTGAG 
GCCACGTGGA GCGCTTACAT CGAAGTCCAG GAATTTGGGG TTCCAGTTCA ACCCCCAAGA 
CCCACCGACC CCAACTTAAT CCCCAGCGCC CCCTCAAAGC CCGAAGTTAC AGATGTCAGC 
AAAAACACGG TCACTTTATC GTGGCAGCCA AACTTGAACT CAGGAGCAAC CCCAACATCT 
TATATTATAG AAGCCTTCAG CCATGCATCT GGTAGCAGCT GGCAGACGGC GGCAGAAAAT 
GTGAAAACAG AAACATTTGC CATCAAAGGA CTCAAACCTA ACGCCATTTA CCTTTTCCTG 
GTGAGGGCAG CAAATGCCTA TGGAATTAGT GATCCAAGCC AAATATCAGA TCCAGTGAAA 
ACGCAAGATG TCCCACCAAC CAGCCAAGGG GTGGACCACA AGCAGGTCCA AAGAGAACTG 
GGGAATGTTG TCTTACATCT GCACAACCCG ACCATCCTTT CTTCCTCCTC AGTCGAAGTG 
CACTGGACGG TGGACCAACA ATCTCAATAT ATTCAAGGCT ATAAAATTCT CTACCGCCCA 
TCTGGAGCCA GCCATGGTGA ATCGGAGTGG TTAGTTTTTG AAGTGAGGAC GCCAACGAAA 
AACAGCGTGG TGATCCCCGA TCTCAGAAAA GGCGTCAACT ACGAGATTAA GGCTCGCCCA 
TTTTTTAATG AGTTTCAAGG AGCAGACAGT GAAATCAAAT TTGCCAAAAC CTTAGAAGAA 
GCACCAAGTG CCCCTCCCCG AAGTGTAACT GTATCCAAGA ATGATGGAAA TGGGACTGCA 
ATCCTGGTCA CTTGGCAACC GCCTCCTGAA GACACACAAA ATGGAATGGT CCAAGAATAT 
AAGGTTTGGT GTCTCGGTAA TGAAACGAAG TATCACATAA ACAAGACAGT CGATGGCTCG 
ACCTTCTCGG TGGTCATCCC CTCTCTGGTT CCTGGGATCC GATACAGTGT GGAGGTGGCA 
GCGAGCACTG GGGCGGGTCC CGGGGTGAAG AGCGAACCTC AGTTCATCCA GTTAGATTCT 
CACGGAAACC CCGTGTCTCC CGAGGACCAA GTCAGCCTTG CTCAGCAGAT CTCCGACGTG 
GTGAGGCAGC CGGCGTTCAT TGCAGGCATT GGGGCAGCCT GTTGGATTAT CCTCATGGTC 
TTCAGCATCT GGCTTTACAG ACACCGCAAG AAGAGAAACG GACTCACCAG CACGTATGCG 
GGTATCCGGA AAGTCCCGTC CTTTACCTTC ACGCCAACAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT 
GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT 
GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC 
AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT 
GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC 
TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG 
TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT 
CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA 
GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG 
GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT 
CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA 
ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG 
GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG 
TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC 
ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG 
CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC 
ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA 
GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG 
ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC 
TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC 
GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT 
GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT 
GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT 
GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG 
GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC 
ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT 
ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA 
GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA 
CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA 
CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA 
ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG 
AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT 
GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522961.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523024.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA.

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CloneID	OMu52701
Clone ID Related Accession (Same CDS sequence)	XM_006522961.2 , XM_006522961.3
Accession Version	XM_006522961.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT 
CGTCAGGAAG ATTTTCCACC CCGCATTGTT GAACACCCTT CAGACCTGAT CGTCTCCAAA 
GGAGAACCTG CCACTCTGAA CTGTAAAGCC GAAGGCCGCC CCACCCCGAC GATAGAATGG 
TACAAAGGTG GGGAAAGAGT GGAGACGGAC AAAGATGACC CGCGCTCACA CCGGATGCTG 
CTGCCAAGCG GGTCTTTATT TTTCTTACGC ATAGTCCATG GACGGAAGAG TAGGCCCGAC 
GAAGGGGTCT ACATCTGTGT GGCTAGGAAT TACCTCGGAG AGGCTGTGAG CCACAACGCA 
TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTCATGGTG 
GCAGTAGGCG AACCTGCAGT CATGGAATGC CAGCCTCCAC GGGGCCATCC AGAGCCCACC 
ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ATGAAAGAAT CACTATACGA 
GGAGGAAAAC TCATGATCAC TTATACGCGT AAAAGCGATG CTGGCAAGTA TGTTTGCGTT 
GGCACCAACA TGGTGGGGGA ACGGGAGAGT GAAGTCGCAG AGCTAACCGT TTTAGAGAGA 
CCATCGTTTG TGAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCAGAATTT 
AAATGTGAGG CCCGAGGAGA CCCTGTGCCT ACAGTTCGAT GGAGGAAAGA TGACGGAGAG 
CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACT 
GCTGGCGACA TGGGATCATA CACTTGTGTG GCAGAAAATA TGGTCGGGAA AGCTGAAGCT 
TCCGCTACTT TGACAGTTCA AGTTGGGTCT GAGCCGCCAC ATTTCGTTGT AAAACCTCGG 
GACCAGGTGG TTGCCTTGGG AAGAACTGTG ACGTTTCAGT GCGAAGCAAC TGGAAATCCT 
CAACCAGCTA TCTTTTGGAG GAGAGAGGGG AGTCAGAATC TACTTTTCTC CTACCAGCCT 
CCACAGTCCT CTAGCCGATT TTCAGTTTCG CAGACAGGAG ACCTCACCAT TACGAACGTC 
CAGCGGTCTG ATGTCGGCTA CTACATCTGT CAGACTTTAA ATGTTGCTGG AAGTATCATC 
ACAAAGGCCT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGG 
CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGCACTT TAATCCTCAG CTGTGTGGCC 
ACAGGCAGTC CAGCACCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA 
GATTCTCGGA TCAAACAGCT GGAGAGTGGC GTGCTGCAGA TCCGCTACGC TAAGCTGGGT 
GACACGGGTC GGTACACCTG CACCGCATCG ACCCCCAGCG GTGAGGCCAC GTGGAGCGCT 
TACATCGAAG TCCAGGAATT TGGGGTTCCA GTTCAACCCC CAAGACCCAC CGACCCCAAC 
TTAATCCCCA GCGCCCCCTC AAAGCCCGAA GTTACAGATG TCAGCAAAAA CACGGTCACT 
TTATCGTGGC AGCCAAACTT GAACTCAGGA GCAACCCCAA CATCTTATAT TATAGAAGCC 
TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACGGCGGCAG AAAATGTGAA AACAGAAACA 
TTTGCCATCA AAGGACTCAA ACCTAACGCC ATTTACCTTT TCCTGGTGAG GGCAGCAAAT 
GCCTATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACGCA AGATGTCCCA 
CCAACCAGCC AAGGGGTGGA CCACAAGCAG GTCCAAAGAG AACTGGGGAA TGTTGTCTTA 
CATCTGCACA ACCCGACCAT CCTTTCTTCC TCCTCAGTCG AAGTGCACTG GACGGTGGAC 
CAACAATCTC AATATATTCA AGGCTATAAA ATTCTCTACC GCCCATCTGG AGCCAGCCAT 
GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACGCCAA CGAAAAACAG CGTGGTGATC 
CCCGATCTCA GAAAAGGCGT CAACTACGAG ATTAAGGCTC GCCCATTTTT TAATGAGTTT 
CAAGGAGCAG ACAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT 
CCCCGAAGTG TAACTGTATC CAAGAATGAT GGAAATGGGA CTGCAATCCT GGTCACTTGG 
CAACCGCCTC CTGAAGACAC ACAAAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC 
GGTAATGAAA CGAAGTATCA CATAAACAAG ACAGTCGATG GCTCGACCTT CTCGGTGGTC 
ATCCCCTCTC TGGTTCCTGG GATCCGATAC AGTGTGGAGG TGGCAGCGAG CACTGGGGCG 
GGTCCCGGGG TGAAGAGCGA ACCTCAGTTC ATCCAGTTAG ATTCTCACGG AAACCCCGTG 
TCTCCCGAGG ACCAAGTCAG CCTTGCTCAG CAGATCTCCG ACGTGGTGAG GCAGCCGGCG 
TTCATTGCAG GCATTGGGGC AGCCTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGCTT 
TACAGACACC GCAAGAAGAG AAACGGACTC ACCAGCACGT ATGCGGGTAT CCGGAAAGTC 
CCGTCCTTTA CCTTCACGCC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT 
GGAGGGAGGC CGGGACTCCT CAACATTAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC 
ACGTGGCCCA ACACAGGCAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACGGCCGGC 
AATGGCAACA GCGACAGCAA CCTGACAACC TACAGTCGCC CAGCTGATTG TATAGCCAAT 
TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT 
GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG 
AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTATGCCAC CACTCAACTC 
ATCCAGGCGA ACCTCAGCAA CAATATGAAT AATGGCGCCG GGGACTCCAG CGAGAAGCAC 
TGGAAGCCTC CGGGACAGCA GAAACCGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG 
CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACGA TCCCTCCAAC CATCCCATAC 
AACCAGTCCT ATGACCAGAA CACAGGAGGG TCTTATAACA GCTCAGATCG GGGCAGTAGT 
ACATCCGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT 
GGCATGAACT GGGCAGACTT GCTGCCCCCT CCTCCGGCGC ATCCTCCCCC ACATAGCAAC 
AGTGAAGAAT ACAACATGTC CGTAGATGAA AGCTATGATC AAGAAATGCC CTGTCCAGTG 
CCACCTGCGC GGATGTATTT GCAACAGGAT GAATTAGAGG AAGAGGAAGA TGAAAGAGGC 
CCCACTCCCC CTGTCCGGGG GGCAGCTTCT TCTCCAGCTG CTGTGTCCTA CAGCCACCAA 
TCCACGGCCA CTCTCACCCC TTCTCCCCAG GAAGAGCTCC AGCCCATGTT ACAGGATTGT 
CCGGAGGACC TCGGGCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC CGTGAGTCCT 
CCTCCACCGC CACGGCCAAT TTCCCCACCT CATACTTACG GCTACATTTC AGGACCCCTT 
GTCTCAGATA TGGATACCGA TGCCCCAGAA GAGGAGGAGG ACGAAGCTGA CATGGAAGTT 
GCCAAAATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTGG AGCAGACCCC AGCATCCAGT 
GTTGGGGACC TTGAGAGCTC CGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCG 
GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCCGATGG CTCCTTTTTC 
ACTGACGCTG ATTTTGCCCA GGCAGTTGCT GCAGCTGCGG AGTATGCGGG CCTGAAAGTG 
GCTCGCCGCC AAATGCAAGA TGCTGCTGGC CGCCGCCACT TCCATGCCTC TCAGTGCCCA 
AGGCCCACGA GTCCTGTGTC CACAGACAGC AACATGAGTG CTGTTGTGAT CCAGAAAGCC 
AGACCCGCCA AGAAGCAGAA ACACCAGCCA GGACATCTGC GCAGGGAAGC CTACGCAGAT 
GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCACTGT CCAGTCCAAG 
GCACAGCTGG AGGTACGGCC TGTCATGGTG CCAAAACTCG CGTCTATAGA AGCAAGGACA 
GATAGATCGT CAGACAGAAA AGGAGGCAGT TACAAGGGGA GAGAAGCTCT GGATGGAAGA 
CAAGTCACTG ACCTGCGAAC AAATCCAAGT GACCCCAGAG AGGCACAGGA ACAGCCAAAT 
GACGGGAAAG GCCGAGGAAC AAAGGCAGCC AAGCGAGACC TTCCACCAGC AAAGACGCAC 
CTCGGCCAAG AGGATATTCT TCCGTACTGT AGACCTACTT TCCCAACGTC AAACAACCCT 
AGAGACCCCA GTTCCTCCAG CTCTATGTCA TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG 
CAAGCAAATG TGGGCCGAAG GAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA 
GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523024.1
CDS	478..5379
Translation	MAVLLLMLLFSMAEITSPVGVSGRMRAGSRWRGEKNRSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522961.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522961.2

ORF Insert Sequence:

ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT 
CGTCAGGAAG ATTTTCCACC CCGCATTGTT GAACACCCTT CAGACCTGAT CGTCTCCAAA 
GGAGAACCTG CCACTCTGAA CTGTAAAGCC GAAGGCCGCC CCACCCCGAC GATAGAATGG 
TACAAAGGTG GGGAAAGAGT GGAGACGGAC AAAGATGACC CGCGCTCACA CCGGATGCTG 
CTGCCAAGCG GGTCTTTATT TTTCTTACGC ATAGTCCATG GACGGAAGAG TAGGCCCGAC 
GAAGGGGTCT ACATCTGTGT GGCTAGGAAT TACCTCGGAG AGGCTGTGAG CCACAACGCA 
TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTCATGGTG 
GCAGTAGGCG AACCTGCAGT CATGGAATGC CAGCCTCCAC GGGGCCATCC AGAGCCCACC 
ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ATGAAAGAAT CACTATACGA 
GGAGGAAAAC TCATGATCAC TTATACGCGT AAAAGCGATG CTGGCAAGTA TGTTTGCGTT 
GGCACCAACA TGGTGGGGGA ACGGGAGAGT GAAGTCGCAG AGCTAACCGT TTTAGAGAGA 
CCATCGTTTG TGAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCAGAATTT 
AAATGTGAGG CCCGAGGAGA CCCTGTGCCT ACAGTTCGAT GGAGGAAAGA TGACGGAGAG 
CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACT 
GCTGGCGACA TGGGATCATA CACTTGTGTG GCAGAAAATA TGGTCGGGAA AGCTGAAGCT 
TCCGCTACTT TGACAGTTCA AGTTGGGTCT GAGCCGCCAC ATTTCGTTGT AAAACCTCGG 
GACCAGGTGG TTGCCTTGGG AAGAACTGTG ACGTTTCAGT GCGAAGCAAC TGGAAATCCT 
CAACCAGCTA TCTTTTGGAG GAGAGAGGGG AGTCAGAATC TACTTTTCTC CTACCAGCCT 
CCACAGTCCT CTAGCCGATT TTCAGTTTCG CAGACAGGAG ACCTCACCAT TACGAACGTC 
CAGCGGTCTG ATGTCGGCTA CTACATCTGT CAGACTTTAA ATGTTGCTGG AAGTATCATC 
ACAAAGGCCT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGG 
CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGCACTT TAATCCTCAG CTGTGTGGCC 
ACAGGCAGTC CAGCACCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA 
GATTCTCGGA TCAAACAGCT GGAGAGTGGC GTGCTGCAGA TCCGCTACGC TAAGCTGGGT 
GACACGGGTC GGTACACCTG CACCGCATCG ACCCCCAGCG GTGAGGCCAC GTGGAGCGCT 
TACATCGAAG TCCAGGAATT TGGGGTTCCA GTTCAACCCC CAAGACCCAC CGACCCCAAC 
TTAATCCCCA GCGCCCCCTC AAAGCCCGAA GTTACAGATG TCAGCAAAAA CACGGTCACT 
TTATCGTGGC AGCCAAACTT GAACTCAGGA GCAACCCCAA CATCTTATAT TATAGAAGCC 
TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACGGCGGCAG AAAATGTGAA AACAGAAACA 
TTTGCCATCA AAGGACTCAA ACCTAACGCC ATTTACCTTT TCCTGGTGAG GGCAGCAAAT 
GCCTATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACGCA AGATGTCCCA 
CCAACCAGCC AAGGGGTGGA CCACAAGCAG GTCCAAAGAG AACTGGGGAA TGTTGTCTTA 
CATCTGCACA ACCCGACCAT CCTTTCTTCC TCCTCAGTCG AAGTGCACTG GACGGTGGAC 
CAACAATCTC AATATATTCA AGGCTATAAA ATTCTCTACC GCCCATCTGG AGCCAGCCAT 
GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACGCCAA CGAAAAACAG CGTGGTGATC 
CCCGATCTCA GAAAAGGCGT CAACTACGAG ATTAAGGCTC GCCCATTTTT TAATGAGTTT 
CAAGGAGCAG ACAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT 
CCCCGAAGTG TAACTGTATC CAAGAATGAT GGAAATGGGA CTGCAATCCT GGTCACTTGG 
CAACCGCCTC CTGAAGACAC ACAAAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC 
GGTAATGAAA CGAAGTATCA CATAAACAAG ACAGTCGATG GCTCGACCTT CTCGGTGGTC 
ATCCCCTCTC TGGTTCCTGG GATCCGATAC AGTGTGGAGG TGGCAGCGAG CACTGGGGCG 
GGTCCCGGGG TGAAGAGCGA ACCTCAGTTC ATCCAGTTAG ATTCTCACGG AAACCCCGTG 
TCTCCCGAGG ACCAAGTCAG CCTTGCTCAG CAGATCTCCG ACGTGGTGAG GCAGCCGGCG 
TTCATTGCAG GCATTGGGGC AGCCTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGCTT 
TACAGACACC GCAAGAAGAG AAACGGACTC ACCAGCACGT ATGCGGGTAT CCGGAAAGTC 
CCGTCCTTTA CCTTCACGCC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT 
GGAGGGAGGC CGGGACTCCT CAACATTAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC 
ACGTGGCCCA ACACAGGCAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACGGCCGGC 
AATGGCAACA GCGACAGCAA CCTGACAACC TACAGTCGCC CAGCTGATTG TATAGCCAAT 
TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT 
GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG 
AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTATGCCAC CACTCAACTC 
ATCCAGGCGA ACCTCAGCAA CAATATGAAT AATGGCGCCG GGGACTCCAG CGAGAAGCAC 
TGGAAGCCTC CGGGACAGCA GAAACCGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG 
CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACGA TCCCTCCAAC CATCCCATAC 
AACCAGTCCT ATGACCAGAA CACAGGAGGG TCTTATAACA GCTCAGATCG GGGCAGTAGT 
ACATCCGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT 
GGCATGAACT GGGCAGACTT GCTGCCCCCT CCTCCGGCGC ATCCTCCCCC ACATAGCAAC 
AGTGAAGAAT ACAACATGTC CGTAGATGAA AGCTATGATC AAGAAATGCC CTGTCCAGTG 
CCACCTGCGC GGATGTATTT GCAACAGGAT GAATTAGAGG AAGAGGAAGA TGAAAGAGGC 
CCCACTCCCC CTGTCCGGGG GGCAGCTTCT TCTCCAGCTG CTGTGTCCTA CAGCCACCAA 
TCCACGGCCA CTCTCACCCC TTCTCCCCAG GAAGAGCTCC AGCCCATGTT ACAGGATTGT 
CCGGAGGACC TCGGGCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC CGTGAGTCCT 
CCTCCACCGC CACGGCCAAT TTCCCCACCT CATACTTACG GCTACATTTC AGGACCCCTT 
GTCTCAGATA TGGATACCGA TGCCCCAGAA GAGGAGGAGG ACGAAGCTGA CATGGAAGTT 
GCCAAAATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTGG AGCAGACCCC AGCATCCAGT 
GTTGGGGACC TTGAGAGCTC CGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCG 
GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCCGATGG CTCCTTTTTC 
ACTGACGCTG ATTTTGCCCA GGCAGTTGCT GCAGCTGCGG AGTATGCGGG CCTGAAAGTG 
GCTCGCCGCC AAATGCAAGA TGCTGCTGGC CGCCGCCACT TCCATGCCTC TCAGTGCCCA 
AGGCCCACGA GTCCTGTGTC CACAGACAGC AACATGAGTG CTGTTGTGAT CCAGAAAGCC 
AGACCCGCCA AGAAGCAGAA ACACCAGCCA GGACATCTGC GCAGGGAAGC CTACGCAGAT 
GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCACTGT CCAGTCCAAG 
GCACAGCTGG AGGTACGGCC TGTCATGGTG CCAAAACTCG CGTCTATAGA AGCAAGGACA 
GATAGATCGT CAGACAGAAA AGGAGGCAGT TACAAGGGGA GAGAAGCTCT GGATGGAAGA 
CAAGTCACTG ACCTGCGAAC AAATCCAAGT GACCCCAGAG AGGCACAGGA ACAGCCAAAT 
GACGGGAAAG GCCGAGGAAC AAAGGCAGCC AAGCGAGACC TTCCACCAGC AAAGACGCAC 
CTCGGCCAAG AGGATATTCT TCCGTACTGT AGACCTACTT TCCCAACGTC AAACAACCCT 
AGAGACCCCA GTTCCTCCAG CTCTATGTCA TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG 
CAAGCAAATG TGGGCCGAAG GAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA 
GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522962.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523025.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA.

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CloneID	OMu52702
Clone ID Related Accession (Same CDS sequence)	XM_006522962.4 , XM_006522962.3
Accession Version	XM_006522962.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522962.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG 
CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC 
CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC 
AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC 
AACCTGACAA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC 
CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG 
AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC 
CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA 
TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC 
ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT 
GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA 
CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC 
ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC 
ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG 
GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT 
CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC 
TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC 
AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT 
GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA 
GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT 
GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT 
CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG 
CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA 
CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT 
CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA 
CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT 
AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA 
GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC 
GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC 
GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA 
GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA 
GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA 
GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523025.1
CDS	97..4995
Translation	MVLRPARGSVCCRKGKEDVCCVLSLCVFLSHVDLLVSGHAVIEMATTVTSVTPSAALVLFPTDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522962.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522962.3

ORF Insert Sequence:

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG 
CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC 
CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC 
AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC 
AACCTGACAA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC 
CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG 
AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC 
CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA 
TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC 
ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT 
GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA 
CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC 
ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC 
ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG 
GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT 
CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC 
TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC 
AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT 
GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA 
GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT 
GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT 
CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG 
CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA 
CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT 
CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA 
CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT 
AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA 
GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC 
GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC 
GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA 
GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA 
GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA 
GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522963.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523026.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA.

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CloneID	OMu52703
Clone ID Related Accession (Same CDS sequence)	XM_006522963.3 , XM_006522963.4
Accession Version	XM_006522963.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG GCTCCCGTCT TCGTCAGGAA 
GATTTTCCAC CCCGCATTGT TGAACACCCT TCAGACCTGA TCGTCTCCAA AGGAGAACCT 
GCCACTCTGA ACTGTAAAGC CGAAGGCCGC CCCACCCCGA CGATAGAATG GTACAAAGGT 
GGGGAAAGAG TGGAGACGGA CAAAGATGAC CCGCGCTCAC ACCGGATGCT GCTGCCAAGC 
GGGTCTTTAT TTTTCTTACG CATAGTCCAT GGACGGAAGA GTAGGCCCGA CGAAGGGGTC 
TACATCTGTG TGGCTAGGAA TTACCTCGGA GAGGCTGTGA GCCACAACGC ATCGCTGGAG 
GTAGCTATAC TACGGGATGA CTTCAGACAA AACCCTTCAG ATGTCATGGT GGCAGTAGGC 
GAACCTGCAG TCATGGAATG CCAGCCTCCA CGGGGCCATC CAGAGCCCAC CATCTCCTGG 
AAAAAGGATG GCTCACCCCT GGATGATAAA GATGAAAGAA TCACTATACG AGGAGGAAAA 
CTCATGATCA CTTATACGCG TAAAAGCGAT GCTGGCAAGT ATGTTTGCGT TGGCACCAAC 
ATGGTGGGGG AACGGGAGAG TGAAGTCGCA GAGCTAACCG TTTTAGAGAG ACCATCGTTT 
GTGAAGAGAC CCAGTAATTT GGCAGTGACT GTGGATGACA GTGCAGAATT TAAATGTGAG 
GCCCGAGGAG ACCCTGTGCC TACAGTTCGA TGGAGGAAAG ATGACGGAGA GCTGCCCAAA 
TCCAGATATG AAATCCGAGA TGATCATACT TTGAAAATTC GGAAGGTGAC TGCTGGCGAC 
ATGGGATCAT ACACTTGTGT GGCAGAAAAT ATGGTCGGGA AAGCTGAAGC TTCCGCTACT 
TTGACAGTTC AAGTTGGGTC TGAGCCGCCA CATTTCGTTG TAAAACCTCG GGACCAGGTG 
GTTGCCTTGG GAAGAACTGT GACGTTTCAG TGCGAAGCAA CTGGAAATCC TCAACCAGCT 
ATCTTTTGGA GGAGAGAGGG GAGTCAGAAT CTACTTTTCT CCTACCAGCC TCCACAGTCC 
TCTAGCCGAT TTTCAGTTTC GCAGACAGGA GACCTCACCA TTACGAACGT CCAGCGGTCT 
GATGTCGGCT ACTACATCTG TCAGACTTTA AATGTTGCTG GAAGTATCAT CACAAAGGCC 
TATTTGGAAG TTACTGATGT GATTGCAGAC CGGCCTCCCC CAGTCATTCG GCAAGGTCCT 
GTGAATCAGA CTGTAGCAGT GGATGGCACT TTAATCCTCA GCTGTGTGGC CACAGGCAGT 
CCAGCACCCA CAATTCTGTG GAGGAAGGAT GGAGTCCTTG TTTCAACCCA AGATTCTCGG 
ATCAAACAGC TGGAGAGTGG CGTGCTGCAG ATCCGCTACG CTAAGCTGGG TGACACGGGT 
CGGTACACCT GCACCGCATC GACCCCCAGC GGTGAGGCCA CGTGGAGCGC TTACATCGAA 
GTCCAGGAAT TTGGGGTTCC AGTTCAACCC CCAAGACCCA CCGACCCCAA CTTAATCCCC 
AGCGCCCCCT CAAAGCCCGA AGTTACAGAT GTCAGCAAAA ACACGGTCAC TTTATCGTGG 
CAGCCAAACT TGAACTCAGG AGCAACCCCA ACATCTTATA TTATAGAAGC CTTCAGCCAT 
GCATCTGGTA GCAGCTGGCA GACGGCGGCA GAAAATGTGA AAACAGAAAC ATTTGCCATC 
AAAGGACTCA AACCTAACGC CATTTACCTT TTCCTGGTGA GGGCAGCAAA TGCCTATGGA 
ATTAGTGATC CAAGCCAAAT ATCAGATCCA GTGAAAACGC AAGATGTCCC ACCAACCAGC 
CAAGGGGTGG ACCACAAGCA GGTCCAAAGA GAACTGGGGA ATGTTGTCTT ACATCTGCAC 
AACCCGACCA TCCTTTCTTC CTCCTCAGTC GAAGTGCACT GGACGGTGGA CCAACAATCT 
CAATATATTC AAGGCTATAA AATTCTCTAC CGCCCATCTG GAGCCAGCCA TGGTGAATCG 
GAGTGGTTAG TTTTTGAAGT GAGGACGCCA ACGAAAAACA GCGTGGTGAT CCCCGATCTC 
AGAAAAGGCG TCAACTACGA GATTAAGGCT CGCCCATTTT TTAATGAGTT TCAAGGAGCA 
GACAGTGAAA TCAAATTTGC CAAAACCTTA GAAGAAGCAC CAAGTGCCCC TCCCCGAAGT 
GTAACTGTAT CCAAGAATGA TGGAAATGGG ACTGCAATCC TGGTCACTTG GCAACCGCCT 
CCTGAAGACA CACAAAATGG AATGGTCCAA GAATATAAGG TTTGGTGTCT CGGTAATGAA 
ACGAAGTATC ACATAAACAA GACAGTCGAT GGCTCGACCT TCTCGGTGGT CATCCCCTCT 
CTGGTTCCTG GGATCCGATA CAGTGTGGAG GTGGCAGCGA GCACTGGGGC GGGTCCCGGG 
GTGAAGAGCG AACCTCAGTT CATCCAGTTA GATTCTCACG GAAACCCCGT GTCTCCCGAG 
GACCAAGTCA GCCTTGCTCA GCAGATCTCC GACGTGGTGA GGCAGCCGGC GTTCATTGCA 
GGCATTGGGG CAGCCTGTTG GATTATCCTC ATGGTCTTCA GCATCTGGCT TTACAGACAC 
CGCAAGAAGA GAAACGGACT CACCAGCACG TATGCGGGTA TCCGGAAAGT CCCGTCCTTT 
ACCTTCACGC CAACAGTAAC TTATCAAAGA GGAGGCGAAG CCGTCAGCAG TGGAGGGAGG 
CCGGGACTCC TCAACATTAG TGAACCTGCC ACGCAGCCAT GGCTGGCAGA CACGTGGCCC 
AACACAGGCA ACAACCACAA TGACTGCTCC ATCAACTGCT GCACGGCCGG CAATGGCAAC 
AGCGACAGCA ACCTGACAAC CTACAGTCGC CCAGCTGATT GTATAGCCAA TTATAACAAC 
CAACTGGATA ACAAACAAAC AAATCTGATG CTCCCTGAGT CAACTGTTTA TGGTGATGTG 
GACCTTAGTA ACAAAATCAA TGAGATGAAA ACCTTCAATA GCCCAAATCT GAAGGACGGG 
CGTTTTGTCA ATCCATCAGG GCAGCCCACT CCCTATGCCA CCACTCAACT CATCCAGGCG 
AACCTCAGCA ACAATATGAA TAATGGCGCC GGGGACTCCA GCGAGAAGCA CTGGAAGCCT 
CCGGGACAGC AGAAACCGGA AGTGGCACCA ATTCAGTATA ACATCATGGA GCAAAACAAG 
CTGAACAAAG ATTATCGGGC AAATGACACG ATCCCTCCAA CCATCCCATA CAACCAGTCC 
TATGACCAGA ACACAGGAGG GTCTTATAAC AGCTCAGATC GGGGCAGTAG TACATCCGGG 
AGTCAAGGCC ACAAGAAAGG GGCACGGACA CCAAAGGCAC CCAAGCAGGG TGGCATGAAC 
TGGGCAGACT TGCTGCCCCC TCCTCCGGCG CATCCTCCCC CACATAGCAA CAGTGAAGAA 
TACAACATGT CCGTAGATGA AAGCTATGAT CAAGAAATGC CCTGTCCAGT GCCACCTGCG 
CGGATGTATT TGCAACAGGA TGAATTAGAG GAAGAGGAAG ATGAAAGAGG CCCCACTCCC 
CCTGTCCGGG GGGCAGCTTC TTCTCCAGCT GCTGTGTCCT ACAGCCACCA ATCCACGGCC 
ACTCTCACCC CTTCTCCCCA GGAAGAGCTC CAGCCCATGT TACAGGATTG TCCGGAGGAC 
CTCGGGCACA TGCCCCACCC ACCAGACAGG AGGCGGCAGC CCGTGAGTCC TCCTCCACCG 
CCACGGCCAA TTTCCCCACC TCATACTTAC GGCTACATTT CAGGACCCCT TGTCTCAGAT 
ATGGATACCG ATGCCCCAGA AGAGGAGGAG GACGAAGCTG ACATGGAAGT TGCCAAAATG 
CAGACCCGAA GGCTGTTGTT ACGTGGGCTG GAGCAGACCC CAGCATCCAG TGTTGGGGAC 
CTTGAGAGCT CCGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC GGAAGAGGAC 
AACATTTCCA GTGGGCGCTC CAGTGTCAGT TCATCCGATG GCTCCTTTTT CACTGACGCT 
GATTTTGCCC AGGCAGTTGC TGCAGCTGCG GAGTATGCGG GCCTGAAAGT GGCTCGCCGC 
CAAATGCAAG ATGCTGCTGG CCGCCGCCAC TTCCATGCCT CTCAGTGCCC AAGGCCCACG 
AGTCCTGTGT CCACAGACAG CAACATGAGT GCTGTTGTGA TCCAGAAAGC CAGACCCGCC 
AAGAAGCAGA AACACCAGCC AGGACATCTG CGCAGGGAAG CCTACGCAGA TGATCTTCCA 
CCCCCTCCAG TGCCACCACC TGCTATAAAA TCGCCCACTG TCCAGTCCAA GGCACAGCTG 
GAGGTACGGC CTGTCATGGT GCCAAAACTC GCGTCTATAG AAGCAAGGAC AGATAGATCG 
TCAGACAGAA AAGGAGGCAG TTACAAGGGG AGAGAAGCTC TGGATGGAAG ACAAGTCACT 
GACCTGCGAA CAAATCCAAG TGACCCCAGA GAGGCACAGG AACAGCCAAA TGACGGGAAA 
GGCCGAGGAA CAAAGGCAGC CAAGCGAGAC CTTCCACCAG CAAAGACGCA CCTCGGCCAA 
GAGGATATTC TTCCGTACTG TAGACCTACT TTCCCAACGT CAAACAACCC TAGAGACCCC 
AGTTCCTCCA GCTCTATGTC ATCAAGAGGA TCAGGGAGCC GACAAAGAGA GCAAGCAAAT 
GTGGGCCGAA GGAATATGGC AGAAATGCAA GTACTTGGGG GATTTGAAAG AGGAGATGAG 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523026.1
CDS	392..5284
Translation	MGGIKMKWKHLPLLVISLLTLSRKQLLLAQLIPGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522963.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522963.3

ORF Insert Sequence:

ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG GCTCCCGTCT TCGTCAGGAA 
GATTTTCCAC CCCGCATTGT TGAACACCCT TCAGACCTGA TCGTCTCCAA AGGAGAACCT 
GCCACTCTGA ACTGTAAAGC CGAAGGCCGC CCCACCCCGA CGATAGAATG GTACAAAGGT 
GGGGAAAGAG TGGAGACGGA CAAAGATGAC CCGCGCTCAC ACCGGATGCT GCTGCCAAGC 
GGGTCTTTAT TTTTCTTACG CATAGTCCAT GGACGGAAGA GTAGGCCCGA CGAAGGGGTC 
TACATCTGTG TGGCTAGGAA TTACCTCGGA GAGGCTGTGA GCCACAACGC ATCGCTGGAG 
GTAGCTATAC TACGGGATGA CTTCAGACAA AACCCTTCAG ATGTCATGGT GGCAGTAGGC 
GAACCTGCAG TCATGGAATG CCAGCCTCCA CGGGGCCATC CAGAGCCCAC CATCTCCTGG 
AAAAAGGATG GCTCACCCCT GGATGATAAA GATGAAAGAA TCACTATACG AGGAGGAAAA 
CTCATGATCA CTTATACGCG TAAAAGCGAT GCTGGCAAGT ATGTTTGCGT TGGCACCAAC 
ATGGTGGGGG AACGGGAGAG TGAAGTCGCA GAGCTAACCG TTTTAGAGAG ACCATCGTTT 
GTGAAGAGAC CCAGTAATTT GGCAGTGACT GTGGATGACA GTGCAGAATT TAAATGTGAG 
GCCCGAGGAG ACCCTGTGCC TACAGTTCGA TGGAGGAAAG ATGACGGAGA GCTGCCCAAA 
TCCAGATATG AAATCCGAGA TGATCATACT TTGAAAATTC GGAAGGTGAC TGCTGGCGAC 
ATGGGATCAT ACACTTGTGT GGCAGAAAAT ATGGTCGGGA AAGCTGAAGC TTCCGCTACT 
TTGACAGTTC AAGTTGGGTC TGAGCCGCCA CATTTCGTTG TAAAACCTCG GGACCAGGTG 
GTTGCCTTGG GAAGAACTGT GACGTTTCAG TGCGAAGCAA CTGGAAATCC TCAACCAGCT 
ATCTTTTGGA GGAGAGAGGG GAGTCAGAAT CTACTTTTCT CCTACCAGCC TCCACAGTCC 
TCTAGCCGAT TTTCAGTTTC GCAGACAGGA GACCTCACCA TTACGAACGT CCAGCGGTCT 
GATGTCGGCT ACTACATCTG TCAGACTTTA AATGTTGCTG GAAGTATCAT CACAAAGGCC 
TATTTGGAAG TTACTGATGT GATTGCAGAC CGGCCTCCCC CAGTCATTCG GCAAGGTCCT 
GTGAATCAGA CTGTAGCAGT GGATGGCACT TTAATCCTCA GCTGTGTGGC CACAGGCAGT 
CCAGCACCCA CAATTCTGTG GAGGAAGGAT GGAGTCCTTG TTTCAACCCA AGATTCTCGG 
ATCAAACAGC TGGAGAGTGG CGTGCTGCAG ATCCGCTACG CTAAGCTGGG TGACACGGGT 
CGGTACACCT GCACCGCATC GACCCCCAGC GGTGAGGCCA CGTGGAGCGC TTACATCGAA 
GTCCAGGAAT TTGGGGTTCC AGTTCAACCC CCAAGACCCA CCGACCCCAA CTTAATCCCC 
AGCGCCCCCT CAAAGCCCGA AGTTACAGAT GTCAGCAAAA ACACGGTCAC TTTATCGTGG 
CAGCCAAACT TGAACTCAGG AGCAACCCCA ACATCTTATA TTATAGAAGC CTTCAGCCAT 
GCATCTGGTA GCAGCTGGCA GACGGCGGCA GAAAATGTGA AAACAGAAAC ATTTGCCATC 
AAAGGACTCA AACCTAACGC CATTTACCTT TTCCTGGTGA GGGCAGCAAA TGCCTATGGA 
ATTAGTGATC CAAGCCAAAT ATCAGATCCA GTGAAAACGC AAGATGTCCC ACCAACCAGC 
CAAGGGGTGG ACCACAAGCA GGTCCAAAGA GAACTGGGGA ATGTTGTCTT ACATCTGCAC 
AACCCGACCA TCCTTTCTTC CTCCTCAGTC GAAGTGCACT GGACGGTGGA CCAACAATCT 
CAATATATTC AAGGCTATAA AATTCTCTAC CGCCCATCTG GAGCCAGCCA TGGTGAATCG 
GAGTGGTTAG TTTTTGAAGT GAGGACGCCA ACGAAAAACA GCGTGGTGAT CCCCGATCTC 
AGAAAAGGCG TCAACTACGA GATTAAGGCT CGCCCATTTT TTAATGAGTT TCAAGGAGCA 
GACAGTGAAA TCAAATTTGC CAAAACCTTA GAAGAAGCAC CAAGTGCCCC TCCCCGAAGT 
GTAACTGTAT CCAAGAATGA TGGAAATGGG ACTGCAATCC TGGTCACTTG GCAACCGCCT 
CCTGAAGACA CACAAAATGG AATGGTCCAA GAATATAAGG TTTGGTGTCT CGGTAATGAA 
ACGAAGTATC ACATAAACAA GACAGTCGAT GGCTCGACCT TCTCGGTGGT CATCCCCTCT 
CTGGTTCCTG GGATCCGATA CAGTGTGGAG GTGGCAGCGA GCACTGGGGC GGGTCCCGGG 
GTGAAGAGCG AACCTCAGTT CATCCAGTTA GATTCTCACG GAAACCCCGT GTCTCCCGAG 
GACCAAGTCA GCCTTGCTCA GCAGATCTCC GACGTGGTGA GGCAGCCGGC GTTCATTGCA 
GGCATTGGGG CAGCCTGTTG GATTATCCTC ATGGTCTTCA GCATCTGGCT TTACAGACAC 
CGCAAGAAGA GAAACGGACT CACCAGCACG TATGCGGGTA TCCGGAAAGT CCCGTCCTTT 
ACCTTCACGC CAACAGTAAC TTATCAAAGA GGAGGCGAAG CCGTCAGCAG TGGAGGGAGG 
CCGGGACTCC TCAACATTAG TGAACCTGCC ACGCAGCCAT GGCTGGCAGA CACGTGGCCC 
AACACAGGCA ACAACCACAA TGACTGCTCC ATCAACTGCT GCACGGCCGG CAATGGCAAC 
AGCGACAGCA ACCTGACAAC CTACAGTCGC CCAGCTGATT GTATAGCCAA TTATAACAAC 
CAACTGGATA ACAAACAAAC AAATCTGATG CTCCCTGAGT CAACTGTTTA TGGTGATGTG 
GACCTTAGTA ACAAAATCAA TGAGATGAAA ACCTTCAATA GCCCAAATCT GAAGGACGGG 
CGTTTTGTCA ATCCATCAGG GCAGCCCACT CCCTATGCCA CCACTCAACT CATCCAGGCG 
AACCTCAGCA ACAATATGAA TAATGGCGCC GGGGACTCCA GCGAGAAGCA CTGGAAGCCT 
CCGGGACAGC AGAAACCGGA AGTGGCACCA ATTCAGTATA ACATCATGGA GCAAAACAAG 
CTGAACAAAG ATTATCGGGC AAATGACACG ATCCCTCCAA CCATCCCATA CAACCAGTCC 
TATGACCAGA ACACAGGAGG GTCTTATAAC AGCTCAGATC GGGGCAGTAG TACATCCGGG 
AGTCAAGGCC ACAAGAAAGG GGCACGGACA CCAAAGGCAC CCAAGCAGGG TGGCATGAAC 
TGGGCAGACT TGCTGCCCCC TCCTCCGGCG CATCCTCCCC CACATAGCAA CAGTGAAGAA 
TACAACATGT CCGTAGATGA AAGCTATGAT CAAGAAATGC CCTGTCCAGT GCCACCTGCG 
CGGATGTATT TGCAACAGGA TGAATTAGAG GAAGAGGAAG ATGAAAGAGG CCCCACTCCC 
CCTGTCCGGG GGGCAGCTTC TTCTCCAGCT GCTGTGTCCT ACAGCCACCA ATCCACGGCC 
ACTCTCACCC CTTCTCCCCA GGAAGAGCTC CAGCCCATGT TACAGGATTG TCCGGAGGAC 
CTCGGGCACA TGCCCCACCC ACCAGACAGG AGGCGGCAGC CCGTGAGTCC TCCTCCACCG 
CCACGGCCAA TTTCCCCACC TCATACTTAC GGCTACATTT CAGGACCCCT TGTCTCAGAT 
ATGGATACCG ATGCCCCAGA AGAGGAGGAG GACGAAGCTG ACATGGAAGT TGCCAAAATG 
CAGACCCGAA GGCTGTTGTT ACGTGGGCTG GAGCAGACCC CAGCATCCAG TGTTGGGGAC 
CTTGAGAGCT CCGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC GGAAGAGGAC 
AACATTTCCA GTGGGCGCTC CAGTGTCAGT TCATCCGATG GCTCCTTTTT CACTGACGCT 
GATTTTGCCC AGGCAGTTGC TGCAGCTGCG GAGTATGCGG GCCTGAAAGT GGCTCGCCGC 
CAAATGCAAG ATGCTGCTGG CCGCCGCCAC TTCCATGCCT CTCAGTGCCC AAGGCCCACG 
AGTCCTGTGT CCACAGACAG CAACATGAGT GCTGTTGTGA TCCAGAAAGC CAGACCCGCC 
AAGAAGCAGA AACACCAGCC AGGACATCTG CGCAGGGAAG CCTACGCAGA TGATCTTCCA 
CCCCCTCCAG TGCCACCACC TGCTATAAAA TCGCCCACTG TCCAGTCCAA GGCACAGCTG 
GAGGTACGGC CTGTCATGGT GCCAAAACTC GCGTCTATAG AAGCAAGGAC AGATAGATCG 
TCAGACAGAA AAGGAGGCAG TTACAAGGGG AGAGAAGCTC TGGATGGAAG ACAAGTCACT 
GACCTGCGAA CAAATCCAAG TGACCCCAGA GAGGCACAGG AACAGCCAAA TGACGGGAAA 
GGCCGAGGAA CAAAGGCAGC CAAGCGAGAC CTTCCACCAG CAAAGACGCA CCTCGGCCAA 
GAGGATATTC TTCCGTACTG TAGACCTACT TTCCCAACGT CAAACAACCC TAGAGACCCC 
AGTTCCTCCA GCTCTATGTC ATCAAGAGGA TCAGGGAGCC GACAAAGAGA GCAAGCAAAT 
GTGGGCCGAA GGAATATGGC AGAAATGCAA GTACTTGGGG GATTTGAAAG AGGAGATGAG 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522964.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523027.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA.

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CloneID	OMu52704
Clone ID Related Accession (Same CDS sequence)	XM_006522964.4 , XM_006522964.3
Accession Version	XM_006522964.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522964.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGGG 
CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT 
AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA 
GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA 
AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG 
TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG 
GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT 
CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA 
AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT 
GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT 
TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG 
GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA 
CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT 
CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA 
GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA 
CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG 
TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC 
AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT 
GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC 
CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC 
AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA 
GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT 
GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG 
CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT 
TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT 
GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC 
AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT 
AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA 
TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA 
GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523027.1
CDS	98..4969
Translation	MVLRPARGSVCCRKGKEDVCCVLSLCVFLSHVDLLVSGHAVIEMATTVTSVTPSAALVLFPTDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522964.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522964.3

ORF Insert Sequence:

ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT 
GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT 
CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT 
GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA 
CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG 
AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA 
GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA 
TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT 
GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA 
CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA 
GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT 
GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC 
ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG 
GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA 
CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA 
GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT 
GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA 
TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT 
CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG 
GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG 
AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA 
TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC 
TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA 
GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG 
ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC 
ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG 
CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC 
TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA 
TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC 
TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC 
TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT 
AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC 
AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT 
CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG 
GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC 
ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT 
CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA 
GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC 
GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA 
ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA 
TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC 
ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT 
CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT 
GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC 
GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC 
AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG 
GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG 
AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC 
GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG 
CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC 
TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGGG 
CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT 
AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA 
GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA 
AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG 
TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG 
GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT 
CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA 
AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT 
GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT 
TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG 
GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA 
CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT 
CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA 
GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA 
CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG 
TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC 
AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT 
GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC 
CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC 
AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA 
GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT 
GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG 
CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT 
TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT 
GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC 
AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT 
AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA 
TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA 
GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA 
ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_006522965.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523028.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu52705
Clone ID Related Accession (Same CDS sequence)	XM_006522965.1 , XM_006522965.2
Accession Version	XM_006522965.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC 
TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA 
GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG 
ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG 
CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC 
AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC 
ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG 
CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT 
ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT 
TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA 
GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG 
GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT 
GAAGCTTCCG CTACTTTGAC AGTTCAAGTT GGGTCTGAGC CGCCACATTT CGTTGTAAAA 
CCTCGGGACC AGGTGGTTGC CTTGGGAAGA ACTGTGACGT TTCAGTGCGA AGCAACTGGA 
AATCCTCAAC CAGCTATCTT TTGGAGGAGA GAGGGGAGTC AGAATCTACT TTTCTCCTAC 
CAGCCTCCAC AGTCCTCTAG CCGATTTTCA GTTTCGCAGA CAGGAGACCT CACCATTACG 
AACGTCCAGC GGTCTGATGT CGGCTACTAC ATCTGTCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AGGCCTATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC 
ATTCGGCAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GCACTTTAAT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGTGGCGTGC TGCAGATCCG CTACGCTAAG 
CTGGGTGACA CGGGTCGGTA CACCTGCACC GCATCGACCC CCAGCGGTGA GGCCACGTGG 
AGCGCTTACA TCGAAGTCCA GGAATTTGGG GTTCCAGTTC AACCCCCAAG ACCCACCGAC 
CCCAACTTAA TCCCCAGCGC CCCCTCAAAG CCCGAAGTTA CAGATGTCAG CAAAAACACG 
GTCACTTTAT CGTGGCAGCC AAACTTGAAC TCAGGAGCAA CCCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACGG CGGCAGAAAA TGTGAAAACA 
GAAACATTTG CCATCAAAGG ACTCAAACCT AACGCCATTT ACCTTTTCCT GGTGAGGGCA 
GCAAATGCCT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACGCAAGAT 
GTCCCACCAA CCAGCCAAGG GGTGGACCAC AAGCAGGTCC AAAGAGAACT GGGGAATGTT 
GTCTTACATC TGCACAACCC GACCATCCTT TCTTCCTCCT CAGTCGAAGT GCACTGGACG 
GTGGACCAAC AATCTCAATA TATTCAAGGC TATAAAATTC TCTACCGCCC ATCTGGAGCC 
AGCCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CGCCAACGAA AAACAGCGTG 
GTGATCCCCG ATCTCAGAAA AGGCGTCAAC TACGAGATTA AGGCTCGCCC ATTTTTTAAT 
GAGTTTCAAG GAGCAGACAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT 
GCCCCTCCCC GAAGTGTAAC TGTATCCAAG AATGATGGAA ATGGGACTGC AATCCTGGTC 
ACTTGGCAAC CGCCTCCTGA AGACACACAA AATGGAATGG TCCAAGAATA TAAGGTTTGG 
TGTCTCGGTA ATGAAACGAA GTATCACATA AACAAGACAG TCGATGGCTC GACCTTCTCG 
GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGATACAGTG TGGAGGTGGC AGCGAGCACT 
GGGGCGGGTC CCGGGGTGAA GAGCGAACCT CAGTTCATCC AGTTAGATTC TCACGGAAAC 
CCCGTGTCTC CCGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCCGACGT GGTGAGGCAG 
CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC TGTTGGATTA TCCTCATGGT CTTCAGCATC 
TGGCTTTACA GACACCGCAA GAAGAGAAAC GGACTCACCA GCACGTATGC GGGTATCCGG 
AAAGTCCCGT CCTTTACCTT CACGCCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC 
AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATTAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCCAACAC AGGCAACAAC CACAATGACT GCTCCATCAA CTGCTGCACG 
GCCGGCAATG GCAACAGCGA CAGCAACCTG ACAACCTACA GTCGCCCAGC TGATTGTATA 
GCCAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA TGCCACCACT 
CAACTCATCC AGGCGAACCT CAGCAACAAT ATGAATAATG GCGCCGGGGA CTCCAGCGAG 
AAGCACTGGA AGCCTCCGGG ACAGCAGAAA CCGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACGATCCC TCCAACCATC 
CCATACAACC AGTCCTATGA CCAGAACACA GGAGGGTCTT ATAACAGCTC AGATCGGGGC 
AGTAGTACAT CCGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG 
CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCTC CGGCGCATCC TCCCCCACAT 
AGCAACAGTG AAGAATACAA CATGTCCGTA GATGAAAGCT ATGATCAAGA AATGCCCTGT 
CCAGTGCCAC CTGCGCGGAT GTATTTGCAA CAGGATGAAT TAGAGGAAGA GGAAGATGAA 
AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA GCTTCTTCTC CAGCTGCTGT GTCCTACAGC 
CACCAATCCA CGGCCACTCT CACCCCTTCT CCCCAGGAAG AGCTCCAGCC CATGTTACAG 
GATTGTCCGG AGGACCTCGG GCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCCGTG 
AGTCCTCCTC CACCGCCACG GCCAATTTCC CCACCTCATA CTTACGGCTA CATTTCAGGA 
CCCCTTGTCT CAGATATGGA TACCGATGCC CCAGAAGAGG AGGAGGACGA AGCTGACATG 
GAAGTTGCCA AAATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTGGAGCA GACCCCAGCA 
TCCAGTGTTG GGGACCTTGA GAGCTCCGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA 
GCCTCGGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC CGATGGCTCC 
TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG 
AAAGTGGCTC GCCGCCAAAT GCAAGATGCT GCTGGCCGCC GCCACTTCCA TGCCTCTCAG 
TGCCCAAGGC CCACGAGTCC TGTGTCCACA GACAGCAACA TGAGTGCTGT TGTGATCCAG 
AAAGCCAGAC CCGCCAAGAA GCAGAAACAC CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC 
GCAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CACTGTCCAG 
TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC ATGGTGCCAA AACTCGCGTC TATAGAAGCA 
AGGACAGATA GATCGTCAGA CAGAAAAGGA GGCAGTTACA AGGGGAGAGA AGCTCTGGAT 
GGAAGACAAG TCACTGACCT GCGAACAAAT CCAAGTGACC CCAGAGAGGC ACAGGAACAG 
CCAAATGACG GGAAAGGCCG AGGAACAAAG GCAGCCAAGC GAGACCTTCC ACCAGCAAAG 
ACGCACCTCG GCCAAGAGGA TATTCTTCCG TACTGTAGAC CTACTTTCCC AACGTCAAAC 
AACCCTAGAG ACCCCAGTTC CTCCAGCTCT ATGTCATCAA GAGGATCAGG GAGCCGACAA 
AGAGAGCAAG CAAATGTGGG CCGAAGGAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT 
GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523028.1
CDS	1107..5954
Translation	MIAEPARFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_006522965.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522965.1

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC 
TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA 
GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG 
ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG 
CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC 
AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC 
ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG 
CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT 
ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT 
TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA 
GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG 
GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT 
GAAGCTTCCG CTACTTTGAC AGTTCAAGTT GGGTCTGAGC CGCCACATTT CGTTGTAAAA 
CCTCGGGACC AGGTGGTTGC CTTGGGAAGA ACTGTGACGT TTCAGTGCGA AGCAACTGGA 
AATCCTCAAC CAGCTATCTT TTGGAGGAGA GAGGGGAGTC AGAATCTACT TTTCTCCTAC 
CAGCCTCCAC AGTCCTCTAG CCGATTTTCA GTTTCGCAGA CAGGAGACCT CACCATTACG 
AACGTCCAGC GGTCTGATGT CGGCTACTAC ATCTGTCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AGGCCTATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC 
ATTCGGCAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GCACTTTAAT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGTGGCGTGC TGCAGATCCG CTACGCTAAG 
CTGGGTGACA CGGGTCGGTA CACCTGCACC GCATCGACCC CCAGCGGTGA GGCCACGTGG 
AGCGCTTACA TCGAAGTCCA GGAATTTGGG GTTCCAGTTC AACCCCCAAG ACCCACCGAC 
CCCAACTTAA TCCCCAGCGC CCCCTCAAAG CCCGAAGTTA CAGATGTCAG CAAAAACACG 
GTCACTTTAT CGTGGCAGCC AAACTTGAAC TCAGGAGCAA CCCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACGG CGGCAGAAAA TGTGAAAACA 
GAAACATTTG CCATCAAAGG ACTCAAACCT AACGCCATTT ACCTTTTCCT GGTGAGGGCA 
GCAAATGCCT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACGCAAGAT 
GTCCCACCAA CCAGCCAAGG GGTGGACCAC AAGCAGGTCC AAAGAGAACT GGGGAATGTT 
GTCTTACATC TGCACAACCC GACCATCCTT TCTTCCTCCT CAGTCGAAGT GCACTGGACG 
GTGGACCAAC AATCTCAATA TATTCAAGGC TATAAAATTC TCTACCGCCC ATCTGGAGCC 
AGCCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CGCCAACGAA AAACAGCGTG 
GTGATCCCCG ATCTCAGAAA AGGCGTCAAC TACGAGATTA AGGCTCGCCC ATTTTTTAAT 
GAGTTTCAAG GAGCAGACAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT 
GCCCCTCCCC GAAGTGTAAC TGTATCCAAG AATGATGGAA ATGGGACTGC AATCCTGGTC 
ACTTGGCAAC CGCCTCCTGA AGACACACAA AATGGAATGG TCCAAGAATA TAAGGTTTGG 
TGTCTCGGTA ATGAAACGAA GTATCACATA AACAAGACAG TCGATGGCTC GACCTTCTCG 
GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGATACAGTG TGGAGGTGGC AGCGAGCACT 
GGGGCGGGTC CCGGGGTGAA GAGCGAACCT CAGTTCATCC AGTTAGATTC TCACGGAAAC 
CCCGTGTCTC CCGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCCGACGT GGTGAGGCAG 
CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC TGTTGGATTA TCCTCATGGT CTTCAGCATC 
TGGCTTTACA GACACCGCAA GAAGAGAAAC GGACTCACCA GCACGTATGC GGGTATCCGG 
AAAGTCCCGT CCTTTACCTT CACGCCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC 
AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATTAGTGAAC CTGCCACGCA GCCATGGCTG 
GCAGACACGT GGCCCAACAC AGGCAACAAC CACAATGACT GCTCCATCAA CTGCTGCACG 
GCCGGCAATG GCAACAGCGA CAGCAACCTG ACAACCTACA GTCGCCCAGC TGATTGTATA 
GCCAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA TGCCACCACT 
CAACTCATCC AGGCGAACCT CAGCAACAAT ATGAATAATG GCGCCGGGGA CTCCAGCGAG 
AAGCACTGGA AGCCTCCGGG ACAGCAGAAA CCGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACGATCCC TCCAACCATC 
CCATACAACC AGTCCTATGA CCAGAACACA GGAGGGTCTT ATAACAGCTC AGATCGGGGC 
AGTAGTACAT CCGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG 
CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCTC CGGCGCATCC TCCCCCACAT 
AGCAACAGTG AAGAATACAA CATGTCCGTA GATGAAAGCT ATGATCAAGA AATGCCCTGT 
CCAGTGCCAC CTGCGCGGAT GTATTTGCAA CAGGATGAAT TAGAGGAAGA GGAAGATGAA 
AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA GCTTCTTCTC CAGCTGCTGT GTCCTACAGC 
CACCAATCCA CGGCCACTCT CACCCCTTCT CCCCAGGAAG AGCTCCAGCC CATGTTACAG 
GATTGTCCGG AGGACCTCGG GCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCCGTG 
AGTCCTCCTC CACCGCCACG GCCAATTTCC CCACCTCATA CTTACGGCTA CATTTCAGGA 
CCCCTTGTCT CAGATATGGA TACCGATGCC CCAGAAGAGG AGGAGGACGA AGCTGACATG 
GAAGTTGCCA AAATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTGGAGCA GACCCCAGCA 
TCCAGTGTTG GGGACCTTGA GAGCTCCGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA 
GCCTCGGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC CGATGGCTCC 
TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG 
AAAGTGGCTC GCCGCCAAAT GCAAGATGCT GCTGGCCGCC GCCACTTCCA TGCCTCTCAG 
TGCCCAAGGC CCACGAGTCC TGTGTCCACA GACAGCAACA TGAGTGCTGT TGTGATCCAG 
AAAGCCAGAC CCGCCAAGAA GCAGAAACAC CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC 
GCAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CACTGTCCAG 
TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC ATGGTGCCAA AACTCGCGTC TATAGAAGCA 
AGGACAGATA GATCGTCAGA CAGAAAAGGA GGCAGTTACA AGGGGAGAGA AGCTCTGGAT 
GGAAGACAAG TCACTGACCT GCGAACAAAT CCAAGTGACC CCAGAGAGGC ACAGGAACAG 
CCAAATGACG GGAAAGGCCG AGGAACAAAG GCAGCCAAGC GAGACCTTCC ACCAGCAAAG 
ACGCACCTCG GCCAAGAGGA TATTCTTCCG TACTGTAGAC CTACTTTCCC AACGTCAAAC 
AACCCTAGAG ACCCCAGTTC CTCCAGCTCT ATGTCATCAA GAGGATCAGG GAGCCGACAA 
AGAGAGCAAG CAAATGTGGG CCGAAGGAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT 
GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_011246105.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244407.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu68623
Clone ID Related Accession (Same CDS sequence)	XM_011246105.2
Accession Version	XM_011246105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCACA GCCGCTGTAG ACTCTTGCGT GCAAAGGGCC TGTCATGTCC AGAAGAAACT 
GACCCTGAGG ACCTAGAGAG GGGGAACGAC AACGGAACGC CAGCCCCCAC CTCTGATAAC 
GATGACAATT CGCTGGGCTA CACAGGCTCC CGTCTTCGTC AGGAAGATTT TCCACCCCGC 
ATTGTTGAAC ACCCTTCAGA CCTGATCGTC TCCAAAGGAG AACCTGCCAC TCTGAACTGT 
AAAGCCGAAG GCCGCCCCAC CCCGACGATA GAATGGTACA AAGGTGGGGA AAGAGTGGAG 
ACGGACAAAG ATGACCCGCG CTCACACCGG ATGCTGCTGC CAAGCGGGTC TTTATTTTTC 
TTACGCATAG TCCATGGACG GAAGAGTAGG CCCGACGAAG GGGTCTACAT CTGTGTGGCT 
AGGAATTACC TCGGAGAGGC TGTGAGCCAC AACGCATCGC TGGAGGTAGC TATACTACGG 
GATGACTTCA GACAAAACCC TTCAGATGTC ATGGTGGCAG TAGGCGAACC TGCAGTCATG 
GAATGCCAGC CTCCACGGGG CCATCCAGAG CCCACCATCT CCTGGAAAAA GGATGGCTCA 
CCCCTGGATG ATAAAGATGA AAGAATCACT ATACGAGGAG GAAAACTCAT GATCACTTAT 
ACGCGTAAAA GCGATGCTGG CAAGTATGTT TGCGTTGGCA CCAACATGGT GGGGGAACGG 
GAGAGTGAAG TCGCAGAGCT AACCGTTTTA GAGAGACCAT CGTTTGTGAA GAGACCCAGT 
AATTTGGCAG TGACTGTGGA TGACAGTGCA GAATTTAAAT GTGAGGCCCG AGGAGACCCT 
GTGCCTACAG TTCGATGGAG GAAAGATGAC GGAGAGCTGC CCAAATCCAG ATATGAAATC 
CGAGATGATC ATACTTTGAA AATTCGGAAG GTGACTGCTG GCGACATGGG ATCATACACT 
TGTGTGGCAG AAAATATGGT CGGGAAAGCT GAAGCTTCCG CTACTTTGAC AGTTCAAGTT 
GGGTCTGAGC CGCCACATTT CGTTGTAAAA CCTCGGGACC AGGTGGTTGC CTTGGGAAGA 
ACTGTGACGT TTCAGTGCGA AGCAACTGGA AATCCTCAAC CAGCTATCTT TTGGAGGAGA 
GAGGGGAGTC AGAATCTACT TTTCTCCTAC CAGCCTCCAC AGTCCTCTAG CCGATTTTCA 
GTTTCGCAGA CAGGAGACCT CACCATTACG AACGTCCAGC GGTCTGATGT CGGCTACTAC 
ATCTGTCAGA CTTTAAATGT TGCTGGAAGT ATCATCACAA AGGCCTATTT GGAAGTTACT 
GATGTGATTG CAGACCGGCC TCCCCCAGTC ATTCGGCAAG GTCCTGTGAA TCAGACTGTA 
GCAGTGGATG GCACTTTAAT CCTCAGCTGT GTGGCCACAG GCAGTCCAGC ACCCACAATT 
CTGTGGAGGA AGGATGGAGT CCTTGTTTCA ACCCAAGATT CTCGGATCAA ACAGCTGGAG 
AGTGGCGTGC TGCAGATCCG CTACGCTAAG CTGGGTGACA CGGGTCGGTA CACCTGCACC 
GCATCGACCC CCAGCGGTGA GGCCACGTGG AGCGCTTACA TCGAAGTCCA GGAATTTGGG 
GTTCCAGTTC AACCCCCAAG ACCCACCGAC CCCAACTTAA TCCCCAGCGC CCCCTCAAAG 
CCCGAAGTTA CAGATGTCAG CAAAAACACG GTCACTTTAT CGTGGCAGCC AAACTTGAAC 
TCAGGAGCAA CCCCAACATC TTATATTATA GAAGCCTTCA GCCATGCATC TGGTAGCAGC 
TGGCAGACGG CGGCAGAAAA TGTGAAAACA GAAACATTTG CCATCAAAGG ACTCAAACCT 
AACGCCATTT ACCTTTTCCT GGTGAGGGCA GCAAATGCCT ATGGAATTAG TGATCCAAGC 
CAAATATCAG ATCCAGTGAA AACGCAAGAT GTCCCACCAA CCAGCCAAGG GGTGGACCAC 
AAGCAGGTCC AAAGAGAACT GGGGAATGTT GTCTTACATC TGCACAACCC GACCATCCTT 
TCTTCCTCCT CAGTCGAAGT GCACTGGACG GTGGACCAAC AATCTCAATA TATTCAAGGC 
TATAAAATTC TCTACCGCCC ATCTGGAGCC AGCCATGGTG AATCGGAGTG GTTAGTTTTT 
GAAGTGAGGA CGCCAACGAA AAACAGCGTG GTGATCCCCG ATCTCAGAAA AGGCGTCAAC 
TACGAGATTA AGGCTCGCCC ATTTTTTAAT GAGTTTCAAG GAGCAGACAG TGAAATCAAA 
TTTGCCAAAA CCTTAGAAGA AGCACCAAGT GCCCCTCCCC GAAGTGTAAC TGTATCCAAG 
AATGATGGAA ATGGGACTGC AATCCTGGTC ACTTGGCAAC CGCCTCCTGA AGACACACAA 
AATGGAATGG TCCAAGAATA TAAGGTTTGG TGTCTCGGTA ATGAAACGAA GTATCACATA 
AACAAGACAG TCGATGGCTC GACCTTCTCG GTGGTCATCC CCTCTCTGGT TCCTGGGATC 
CGATACAGTG TGGAGGTGGC AGCGAGCACT GGGGCGGGTC CCGGGGTGAA GAGCGAACCT 
CAGTTCATCC AGTTAGATTC TCACGGAAAC CCCGTGTCTC CCGAGGACCA AGTCAGCCTT 
GCTCAGCAGA TCTCCGACGT GGTGAGGCAG CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC 
TGTTGGATTA TCCTCATGGT CTTCAGCATC TGGCTTTACA GACACCGCAA GAAGAGAAAC 
GGACTCACCA GCACGTATGC GGGTATCCGG AAAGTCCCGT CCTTTACCTT CACGCCAACA 
GTAACTTATC AAAGAGGAGG CGAAGCCGTC AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC 
ATTAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCCAACAC AGGCAACAAC 
CACAATGACT GCTCCATCAA CTGCTGCACG GCCGGCAATG GCAACAGCGA CAGCAACCTG 
ACAACCTACA GTCGCCCAGC TGATTGTATA GCCAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ACGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CCACTCCCTA TGCCACCACT CAACTCATCC AGGCGAACCT CAGCAACAAT 
ATGAATAATG GCGCCGGGGA CTCCAGCGAG AAGCACTGGA AGCCTCCGGG ACAGCAGAAA 
CCGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAGCTGAA CAAAGATTAT 
CGGGCAAATG ACACGATCCC TCCAACCATC CCATACAACC AGTCCTATGA CCAGAACACA 
GGAGGGTCTT ATAACAGCTC AGATCGGGGC AGTAGTACAT CCGGGAGTCA AGGCCACAAG 
AAAGGGGCAC GGACACCAAA GGCACCCAAG CAGGGTGGCA TGAACTGGGC AGACTTGCTG 
CCCCCTCCTC CGGCGCATCC TCCCCCACAT AGCAACAGTG AAGAATACAA CATGTCCGTA 
GATGAAAGCT ATGATCAAGA AATGCCCTGT CCAGTGCCAC CTGCGCGGAT GTATTTGCAA 
CAGGATGAAT TAGAGGAAGA GGAAGATGAA AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA 
GCTTCTTCTC CAGCTGCTGT GTCCTACAGC CACCAATCCA CGGCCACTCT CACCCCTTCT 
CCCCAGGAAG AGCTCCAGCC CATGTTACAG GATTGTCCGG AGGACCTCGG GCACATGCCC 
CACCCACCAG ACAGGAGGCG GCAGCCCGTG AGTCCTCCTC CACCGCCACG GCCAATTTCC 
CCACCTCATA CTTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACCGATGCC 
CCAGAAGAGG AGGAGGACGA AGCTGACATG GAAGTTGCCA AAATGCAGAC CCGAAGGCTG 
TTGTTACGTG GGCTGGAGCA GACCCCAGCA TCCAGTGTTG GGGACCTTGA GAGCTCCGTC 
ACTGGGTCCA TGATCAATGG CTGGGGCTCA GCCTCGGAAG AGGACAACAT TTCCAGTGGG 
CGCTCCAGTG TCAGTTCATC CGATGGCTCC TTTTTCACTG ACGCTGATTT TGCCCAGGCA 
GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG AAAGTGGCTC GCCGCCAAAT GCAAGATGCT 
GCTGGCCGCC GCCACTTCCA TGCCTCTCAG TGCCCAAGGC CCACGAGTCC TGTGTCCACA 
GACAGCAACA TGAGTGCTGT TGTGATCCAG AAAGCCAGAC CCGCCAAGAA GCAGAAACAC 
CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC GCAGATGATC TTCCACCCCC TCCAGTGCCA 
CCACCTGCTA TAAAATCGCC CACTGTCCAG TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC 
ATGGTGCCAA AACTCGCGTC TATAGAAGCA AGGACAGATA GATCGTCAGA CAGAAAAGGA 
GGCAGTTACA AGGGGAGAGA AGCTCTGGAT GGAAGACAAG TCACTGACCT GCGAACAAAT 
CCAAGTGACC CCAGAGAGGC ACAGGAACAG CCAAATGACG GGAAAGGCCG AGGAACAAAG 
GCAGCCAAGC GAGACCTTCC ACCAGCAAAG ACGCACCTCG GCCAAGAGGA TATTCTTCCG 
TACTGTAGAC CTACTTTCCC AACGTCAAAC AACCCTAGAG ACCCCAGTTC CTCCAGCTCT 
ATGTCATCAA GAGGATCAGG GAGCCGACAA AGAGAGCAAG CAAATGTGGG CCGAAGGAAT 
ATGGCAGAAA TGCAAGTACT TGGGGGATTT GAAAGAGGAG ATGAGAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244407.1
CDS	20..4957
Translation	MNHSRCRLLRAKGLSCPEETDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_011246105.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246105.2

ORF Insert Sequence:

ATGAATCACA GCCGCTGTAG ACTCTTGCGT GCAAAGGGCC TGTCATGTCC AGAAGAAACT 
GACCCTGAGG ACCTAGAGAG GGGGAACGAC AACGGAACGC CAGCCCCCAC CTCTGATAAC 
GATGACAATT CGCTGGGCTA CACAGGCTCC CGTCTTCGTC AGGAAGATTT TCCACCCCGC 
ATTGTTGAAC ACCCTTCAGA CCTGATCGTC TCCAAAGGAG AACCTGCCAC TCTGAACTGT 
AAAGCCGAAG GCCGCCCCAC CCCGACGATA GAATGGTACA AAGGTGGGGA AAGAGTGGAG 
ACGGACAAAG ATGACCCGCG CTCACACCGG ATGCTGCTGC CAAGCGGGTC TTTATTTTTC 
TTACGCATAG TCCATGGACG GAAGAGTAGG CCCGACGAAG GGGTCTACAT CTGTGTGGCT 
AGGAATTACC TCGGAGAGGC TGTGAGCCAC AACGCATCGC TGGAGGTAGC TATACTACGG 
GATGACTTCA GACAAAACCC TTCAGATGTC ATGGTGGCAG TAGGCGAACC TGCAGTCATG 
GAATGCCAGC CTCCACGGGG CCATCCAGAG CCCACCATCT CCTGGAAAAA GGATGGCTCA 
CCCCTGGATG ATAAAGATGA AAGAATCACT ATACGAGGAG GAAAACTCAT GATCACTTAT 
ACGCGTAAAA GCGATGCTGG CAAGTATGTT TGCGTTGGCA CCAACATGGT GGGGGAACGG 
GAGAGTGAAG TCGCAGAGCT AACCGTTTTA GAGAGACCAT CGTTTGTGAA GAGACCCAGT 
AATTTGGCAG TGACTGTGGA TGACAGTGCA GAATTTAAAT GTGAGGCCCG AGGAGACCCT 
GTGCCTACAG TTCGATGGAG GAAAGATGAC GGAGAGCTGC CCAAATCCAG ATATGAAATC 
CGAGATGATC ATACTTTGAA AATTCGGAAG GTGACTGCTG GCGACATGGG ATCATACACT 
TGTGTGGCAG AAAATATGGT CGGGAAAGCT GAAGCTTCCG CTACTTTGAC AGTTCAAGTT 
GGGTCTGAGC CGCCACATTT CGTTGTAAAA CCTCGGGACC AGGTGGTTGC CTTGGGAAGA 
ACTGTGACGT TTCAGTGCGA AGCAACTGGA AATCCTCAAC CAGCTATCTT TTGGAGGAGA 
GAGGGGAGTC AGAATCTACT TTTCTCCTAC CAGCCTCCAC AGTCCTCTAG CCGATTTTCA 
GTTTCGCAGA CAGGAGACCT CACCATTACG AACGTCCAGC GGTCTGATGT CGGCTACTAC 
ATCTGTCAGA CTTTAAATGT TGCTGGAAGT ATCATCACAA AGGCCTATTT GGAAGTTACT 
GATGTGATTG CAGACCGGCC TCCCCCAGTC ATTCGGCAAG GTCCTGTGAA TCAGACTGTA 
GCAGTGGATG GCACTTTAAT CCTCAGCTGT GTGGCCACAG GCAGTCCAGC ACCCACAATT 
CTGTGGAGGA AGGATGGAGT CCTTGTTTCA ACCCAAGATT CTCGGATCAA ACAGCTGGAG 
AGTGGCGTGC TGCAGATCCG CTACGCTAAG CTGGGTGACA CGGGTCGGTA CACCTGCACC 
GCATCGACCC CCAGCGGTGA GGCCACGTGG AGCGCTTACA TCGAAGTCCA GGAATTTGGG 
GTTCCAGTTC AACCCCCAAG ACCCACCGAC CCCAACTTAA TCCCCAGCGC CCCCTCAAAG 
CCCGAAGTTA CAGATGTCAG CAAAAACACG GTCACTTTAT CGTGGCAGCC AAACTTGAAC 
TCAGGAGCAA CCCCAACATC TTATATTATA GAAGCCTTCA GCCATGCATC TGGTAGCAGC 
TGGCAGACGG CGGCAGAAAA TGTGAAAACA GAAACATTTG CCATCAAAGG ACTCAAACCT 
AACGCCATTT ACCTTTTCCT GGTGAGGGCA GCAAATGCCT ATGGAATTAG TGATCCAAGC 
CAAATATCAG ATCCAGTGAA AACGCAAGAT GTCCCACCAA CCAGCCAAGG GGTGGACCAC 
AAGCAGGTCC AAAGAGAACT GGGGAATGTT GTCTTACATC TGCACAACCC GACCATCCTT 
TCTTCCTCCT CAGTCGAAGT GCACTGGACG GTGGACCAAC AATCTCAATA TATTCAAGGC 
TATAAAATTC TCTACCGCCC ATCTGGAGCC AGCCATGGTG AATCGGAGTG GTTAGTTTTT 
GAAGTGAGGA CGCCAACGAA AAACAGCGTG GTGATCCCCG ATCTCAGAAA AGGCGTCAAC 
TACGAGATTA AGGCTCGCCC ATTTTTTAAT GAGTTTCAAG GAGCAGACAG TGAAATCAAA 
TTTGCCAAAA CCTTAGAAGA AGCACCAAGT GCCCCTCCCC GAAGTGTAAC TGTATCCAAG 
AATGATGGAA ATGGGACTGC AATCCTGGTC ACTTGGCAAC CGCCTCCTGA AGACACACAA 
AATGGAATGG TCCAAGAATA TAAGGTTTGG TGTCTCGGTA ATGAAACGAA GTATCACATA 
AACAAGACAG TCGATGGCTC GACCTTCTCG GTGGTCATCC CCTCTCTGGT TCCTGGGATC 
CGATACAGTG TGGAGGTGGC AGCGAGCACT GGGGCGGGTC CCGGGGTGAA GAGCGAACCT 
CAGTTCATCC AGTTAGATTC TCACGGAAAC CCCGTGTCTC CCGAGGACCA AGTCAGCCTT 
GCTCAGCAGA TCTCCGACGT GGTGAGGCAG CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC 
TGTTGGATTA TCCTCATGGT CTTCAGCATC TGGCTTTACA GACACCGCAA GAAGAGAAAC 
GGACTCACCA GCACGTATGC GGGTATCCGG AAAGTCCCGT CCTTTACCTT CACGCCAACA 
GTAACTTATC AAAGAGGAGG CGAAGCCGTC AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC 
ATTAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCCAACAC AGGCAACAAC 
CACAATGACT GCTCCATCAA CTGCTGCACG GCCGGCAATG GCAACAGCGA CAGCAACCTG 
ACAACCTACA GTCGCCCAGC TGATTGTATA GCCAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ACGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CCACTCCCTA TGCCACCACT CAACTCATCC AGGCGAACCT CAGCAACAAT 
ATGAATAATG GCGCCGGGGA CTCCAGCGAG AAGCACTGGA AGCCTCCGGG ACAGCAGAAA 
CCGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAGCTGAA CAAAGATTAT 
CGGGCAAATG ACACGATCCC TCCAACCATC CCATACAACC AGTCCTATGA CCAGAACACA 
GGAGGGTCTT ATAACAGCTC AGATCGGGGC AGTAGTACAT CCGGGAGTCA AGGCCACAAG 
AAAGGGGCAC GGACACCAAA GGCACCCAAG CAGGGTGGCA TGAACTGGGC AGACTTGCTG 
CCCCCTCCTC CGGCGCATCC TCCCCCACAT AGCAACAGTG AAGAATACAA CATGTCCGTA 
GATGAAAGCT ATGATCAAGA AATGCCCTGT CCAGTGCCAC CTGCGCGGAT GTATTTGCAA 
CAGGATGAAT TAGAGGAAGA GGAAGATGAA AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA 
GCTTCTTCTC CAGCTGCTGT GTCCTACAGC CACCAATCCA CGGCCACTCT CACCCCTTCT 
CCCCAGGAAG AGCTCCAGCC CATGTTACAG GATTGTCCGG AGGACCTCGG GCACATGCCC 
CACCCACCAG ACAGGAGGCG GCAGCCCGTG AGTCCTCCTC CACCGCCACG GCCAATTTCC 
CCACCTCATA CTTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACCGATGCC 
CCAGAAGAGG AGGAGGACGA AGCTGACATG GAAGTTGCCA AAATGCAGAC CCGAAGGCTG 
TTGTTACGTG GGCTGGAGCA GACCCCAGCA TCCAGTGTTG GGGACCTTGA GAGCTCCGTC 
ACTGGGTCCA TGATCAATGG CTGGGGCTCA GCCTCGGAAG AGGACAACAT TTCCAGTGGG 
CGCTCCAGTG TCAGTTCATC CGATGGCTCC TTTTTCACTG ACGCTGATTT TGCCCAGGCA 
GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG AAAGTGGCTC GCCGCCAAAT GCAAGATGCT 
GCTGGCCGCC GCCACTTCCA TGCCTCTCAG TGCCCAAGGC CCACGAGTCC TGTGTCCACA 
GACAGCAACA TGAGTGCTGT TGTGATCCAG AAAGCCAGAC CCGCCAAGAA GCAGAAACAC 
CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC GCAGATGATC TTCCACCCCC TCCAGTGCCA 
CCACCTGCTA TAAAATCGCC CACTGTCCAG TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC 
ATGGTGCCAA AACTCGCGTC TATAGAAGCA AGGACAGATA GATCGTCAGA CAGAAAAGGA 
GGCAGTTACA AGGGGAGAGA AGCTCTGGAT GGAAGACAAG TCACTGACCT GCGAACAAAT 
CCAAGTGACC CCAGAGAGGC ACAGGAACAG CCAAATGACG GGAAAGGCCG AGGAACAAAG 
GCAGCCAAGC GAGACCTTCC ACCAGCAAAG ACGCACCTCG GCCAAGAGGA TATTCTTCCG 
TACTGTAGAC CTACTTTCCC AACGTCAAAC AACCCTAGAG ACCCCAGTTC CTCCAGCTCT 
ATGTCATCAA GAGGATCAGG GAGCCGACAA AGAGAGCAAG CAAATGTGGG CCGAAGGAAT 
ATGGCAGAAA TGCAAGTACT TGGGGGATTT GAAAGAGGAG ATGAGAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo1 ( XM_011246106.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244408.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA.

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CloneID	OMu68624
Clone ID Related Accession (Same CDS sequence)	XM_011246106.2
Accession Version	XM_011246106.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCACA GCCGCTGTAG ACTCTTGCGT GCAAAGGGCC TGTCATGTCC AGAAGAAACT 
GGCTCCCGTC TTCGTCAGGA AGATTTTCCA CCCCGCATTG TTGAACACCC TTCAGACCTG 
ATCGTCTCCA AAGGAGAACC TGCCACTCTG AACTGTAAAG CCGAAGGCCG CCCCACCCCG 
ACGATAGAAT GGTACAAAGG TGGGGAAAGA GTGGAGACGG ACAAAGATGA CCCGCGCTCA 
CACCGGATGC TGCTGCCAAG CGGGTCTTTA TTTTTCTTAC GCATAGTCCA TGGACGGAAG 
AGTAGGCCCG ACGAAGGGGT CTACATCTGT GTGGCTAGGA ATTACCTCGG AGAGGCTGTG 
AGCCACAACG CATCGCTGGA GGTAGCTATA CTACGGGATG ACTTCAGACA AAACCCTTCA 
GATGTCATGG TGGCAGTAGG CGAACCTGCA GTCATGGAAT GCCAGCCTCC ACGGGGCCAT 
CCAGAGCCCA CCATCTCCTG GAAAAAGGAT GGCTCACCCC TGGATGATAA AGATGAAAGA 
ATCACTATAC GAGGAGGAAA ACTCATGATC ACTTATACGC GTAAAAGCGA TGCTGGCAAG 
TATGTTTGCG TTGGCACCAA CATGGTGGGG GAACGGGAGA GTGAAGTCGC AGAGCTAACC 
GTTTTAGAGA GACCATCGTT TGTGAAGAGA CCCAGTAATT TGGCAGTGAC TGTGGATGAC 
AGTGCAGAAT TTAAATGTGA GGCCCGAGGA GACCCTGTGC CTACAGTTCG ATGGAGGAAA 
GATGACGGAG AGCTGCCCAA ATCCAGATAT GAAATCCGAG ATGATCATAC TTTGAAAATT 
CGGAAGGTGA CTGCTGGCGA CATGGGATCA TACACTTGTG TGGCAGAAAA TATGGTCGGG 
AAAGCTGAAG CTTCCGCTAC TTTGACAGTT CAAGTTGGGT CTGAGCCGCC ACATTTCGTT 
GTAAAACCTC GGGACCAGGT GGTTGCCTTG GGAAGAACTG TGACGTTTCA GTGCGAAGCA 
ACTGGAAATC CTCAACCAGC TATCTTTTGG AGGAGAGAGG GGAGTCAGAA TCTACTTTTC 
TCCTACCAGC CTCCACAGTC CTCTAGCCGA TTTTCAGTTT CGCAGACAGG AGACCTCACC 
ATTACGAACG TCCAGCGGTC TGATGTCGGC TACTACATCT GTCAGACTTT AAATGTTGCT 
GGAAGTATCA TCACAAAGGC CTATTTGGAA GTTACTGATG TGATTGCAGA CCGGCCTCCC 
CCAGTCATTC GGCAAGGTCC TGTGAATCAG ACTGTAGCAG TGGATGGCAC TTTAATCCTC 
AGCTGTGTGG CCACAGGCAG TCCAGCACCC ACAATTCTGT GGAGGAAGGA TGGAGTCCTT 
GTTTCAACCC AAGATTCTCG GATCAAACAG CTGGAGAGTG GCGTGCTGCA GATCCGCTAC 
GCTAAGCTGG GTGACACGGG TCGGTACACC TGCACCGCAT CGACCCCCAG CGGTGAGGCC 
ACGTGGAGCG CTTACATCGA AGTCCAGGAA TTTGGGGTTC CAGTTCAACC CCCAAGACCC 
ACCGACCCCA ACTTAATCCC CAGCGCCCCC TCAAAGCCCG AAGTTACAGA TGTCAGCAAA 
AACACGGTCA CTTTATCGTG GCAGCCAAAC TTGAACTCAG GAGCAACCCC AACATCTTAT 
ATTATAGAAG CCTTCAGCCA TGCATCTGGT AGCAGCTGGC AGACGGCGGC AGAAAATGTG 
AAAACAGAAA CATTTGCCAT CAAAGGACTC AAACCTAACG CCATTTACCT TTTCCTGGTG 
AGGGCAGCAA ATGCCTATGG AATTAGTGAT CCAAGCCAAA TATCAGATCC AGTGAAAACG 
CAAGATGTCC CACCAACCAG CCAAGGGGTG GACCACAAGC AGGTCCAAAG AGAACTGGGG 
AATGTTGTCT TACATCTGCA CAACCCGACC ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC 
TGGACGGTGG ACCAACAATC TCAATATATT CAAGGCTATA AAATTCTCTA CCGCCCATCT 
GGAGCCAGCC ATGGTGAATC GGAGTGGTTA GTTTTTGAAG TGAGGACGCC AACGAAAAAC 
AGCGTGGTGA TCCCCGATCT CAGAAAAGGC GTCAACTACG AGATTAAGGC TCGCCCATTT 
TTTAATGAGT TTCAAGGAGC AGACAGTGAA ATCAAATTTG CCAAAACCTT AGAAGAAGCA 
CCAAGTGCCC CTCCCCGAAG TGTAACTGTA TCCAAGAATG ATGGAAATGG GACTGCAATC 
CTGGTCACTT GGCAACCGCC TCCTGAAGAC ACACAAAATG GAATGGTCCA AGAATATAAG 
GTTTGGTGTC TCGGTAATGA AACGAAGTAT CACATAAACA AGACAGTCGA TGGCTCGACC 
TTCTCGGTGG TCATCCCCTC TCTGGTTCCT GGGATCCGAT ACAGTGTGGA GGTGGCAGCG 
AGCACTGGGG CGGGTCCCGG GGTGAAGAGC GAACCTCAGT TCATCCAGTT AGATTCTCAC 
GGAAACCCCG TGTCTCCCGA GGACCAAGTC AGCCTTGCTC AGCAGATCTC CGACGTGGTG 
AGGCAGCCGG CGTTCATTGC AGGCATTGGG GCAGCCTGTT GGATTATCCT CATGGTCTTC 
AGCATCTGGC TTTACAGACA CCGCAAGAAG AGAAACGGAC TCACCAGCAC GTATGCGGGT 
ATCCGGAAAG TCCCGTCCTT TACCTTCACG CCAACAGTAA CTTATCAAAG AGGAGGCGAA 
GCCGTCAGCA GTGGAGGGAG GCCGGGACTC CTCAACATTA GTGAACCTGC CACGCAGCCA 
TGGCTGGCAG ACACGTGGCC CAACACAGGC AACAACCACA ATGACTGCTC CATCAACTGC 
TGCACGGCCG GCAATGGCAA CAGCGACAGC AACCTGACAA CCTACAGTCG CCCAGCTGAT 
TGTATAGCCA ATTATAACAA CCAACTGGAT AACAAACAAA CAAATCTGAT GCTCCCTGAG 
TCAACTGTTT ATGGTGATGT GGACCTTAGT AACAAAATCA ATGAGATGAA AACCTTCAAT 
AGCCCAAATC TGAAGGACGG GCGTTTTGTC AATCCATCAG GGCAGCCCAC TCCCTATGCC 
ACCACTCAAC TCATCCAGGC GAACCTCAGC AACAATATGA ATAATGGCGC CGGGGACTCC 
AGCGAGAAGC ACTGGAAGCC TCCGGGACAG CAGAAACCGG AAGTGGCACC AATTCAGTAT 
AACATCATGG AGCAAAACAA GCTGAACAAA GATTATCGGG CAAATGACAC GATCCCTCCA 
ACCATCCCAT ACAACCAGTC CTATGACCAG AACACAGGAG GGTCTTATAA CAGCTCAGAT 
CGGGGCAGTA GTACATCCGG GAGTCAAGGC CACAAGAAAG GGGCACGGAC ACCAAAGGCA 
CCCAAGCAGG GTGGCATGAA CTGGGCAGAC TTGCTGCCCC CTCCTCCGGC GCATCCTCCC 
CCACATAGCA ACAGTGAAGA ATACAACATG TCCGTAGATG AAAGCTATGA TCAAGAAATG 
CCCTGTCCAG TGCCACCTGC GCGGATGTAT TTGCAACAGG ATGAATTAGA GGAAGAGGAA 
GATGAAAGAG GCCCCACTCC CCCTGTCCGG GGGGCAGCTT CTTCTCCAGC TGCTGTGTCC 
TACAGCCACC AATCCACGGC CACTCTCACC CCTTCTCCCC AGGAAGAGCT CCAGCCCATG 
TTACAGGATT GTCCGGAGGA CCTCGGGCAC ATGCCCCACC CACCAGACAG GAGGCGGCAG 
CCCGTGAGTC CTCCTCCACC GCCACGGCCA ATTTCCCCAC CTCATACTTA CGGCTACATT 
TCAGGACCCC TTGTCTCAGA TATGGATACC GATGCCCCAG AAGAGGAGGA GGACGAAGCT 
GACATGGAAG TTGCCAAAAT GCAGACCCGA AGGCTGTTGT TACGTGGGCT GGAGCAGACC 
CCAGCATCCA GTGTTGGGGA CCTTGAGAGC TCCGTCACTG GGTCCATGAT CAATGGCTGG 
GGCTCAGCCT CGGAAGAGGA CAACATTTCC AGTGGGCGCT CCAGTGTCAG TTCATCCGAT 
GGCTCCTTTT TCACTGACGC TGATTTTGCC CAGGCAGTTG CTGCAGCTGC GGAGTATGCG 
GGCCTGAAAG TGGCTCGCCG CCAAATGCAA GATGCTGCTG GCCGCCGCCA CTTCCATGCC 
TCTCAGTGCC CAAGGCCCAC GAGTCCTGTG TCCACAGACA GCAACATGAG TGCTGTTGTG 
ATCCAGAAAG CCAGACCCGC CAAGAAGCAG AAACACCAGC CAGGACATCT GCGCAGGGAA 
GCCTACGCAG ATGATCTTCC ACCCCCTCCA GTGCCACCAC CTGCTATAAA ATCGCCCACT 
GTCCAGTCCA AGGCACAGCT GGAGGTACGG CCTGTCATGG TGCCAAAACT CGCGTCTATA 
GAAGCAAGGA CAGATAGATC GTCAGACAGA AAAGGAGGCA GTTACAAGGG GAGAGAAGCT 
CTGGATGGAA GACAAGTCAC TGACCTGCGA ACAAATCCAA GTGACCCCAG AGAGGCACAG 
GAACAGCCAA ATGACGGGAA AGGCCGAGGA ACAAAGGCAG CCAAGCGAGA CCTTCCACCA 
GCAAAGACGC ACCTCGGCCA AGAGGATATT CTTCCGTACT GTAGACCTAC TTTCCCAACG 
TCAAACAACC CTAGAGACCC CAGTTCCTCC AGCTCTATGT CATCAAGAGG ATCAGGGAGC 
CGACAAAGAG AGCAAGCAAA TGTGGGCCGA AGGAATATGG CAGAAATGCA AGTACTTGGG 
GGATTTGAAA GAGGAGATGA GAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244408.1
CDS	16..4869
Translation	MNHSRCRLLRAKGLSCPEETGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES

Target ORF information:

RefSeq Version	XM_011246106.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246106.2

ORF Insert Sequence:

ATGAATCACA GCCGCTGTAG ACTCTTGCGT GCAAAGGGCC TGTCATGTCC AGAAGAAACT 
GGCTCCCGTC TTCGTCAGGA AGATTTTCCA CCCCGCATTG TTGAACACCC TTCAGACCTG 
ATCGTCTCCA AAGGAGAACC TGCCACTCTG AACTGTAAAG CCGAAGGCCG CCCCACCCCG 
ACGATAGAAT GGTACAAAGG TGGGGAAAGA GTGGAGACGG ACAAAGATGA CCCGCGCTCA 
CACCGGATGC TGCTGCCAAG CGGGTCTTTA TTTTTCTTAC GCATAGTCCA TGGACGGAAG 
AGTAGGCCCG ACGAAGGGGT CTACATCTGT GTGGCTAGGA ATTACCTCGG AGAGGCTGTG 
AGCCACAACG CATCGCTGGA GGTAGCTATA CTACGGGATG ACTTCAGACA AAACCCTTCA 
GATGTCATGG TGGCAGTAGG CGAACCTGCA GTCATGGAAT GCCAGCCTCC ACGGGGCCAT 
CCAGAGCCCA CCATCTCCTG GAAAAAGGAT GGCTCACCCC TGGATGATAA AGATGAAAGA 
ATCACTATAC GAGGAGGAAA ACTCATGATC ACTTATACGC GTAAAAGCGA TGCTGGCAAG 
TATGTTTGCG TTGGCACCAA CATGGTGGGG GAACGGGAGA GTGAAGTCGC AGAGCTAACC 
GTTTTAGAGA GACCATCGTT TGTGAAGAGA CCCAGTAATT TGGCAGTGAC TGTGGATGAC 
AGTGCAGAAT TTAAATGTGA GGCCCGAGGA GACCCTGTGC CTACAGTTCG ATGGAGGAAA 
GATGACGGAG AGCTGCCCAA ATCCAGATAT GAAATCCGAG ATGATCATAC TTTGAAAATT 
CGGAAGGTGA CTGCTGGCGA CATGGGATCA TACACTTGTG TGGCAGAAAA TATGGTCGGG 
AAAGCTGAAG CTTCCGCTAC TTTGACAGTT CAAGTTGGGT CTGAGCCGCC ACATTTCGTT 
GTAAAACCTC GGGACCAGGT GGTTGCCTTG GGAAGAACTG TGACGTTTCA GTGCGAAGCA 
ACTGGAAATC CTCAACCAGC TATCTTTTGG AGGAGAGAGG GGAGTCAGAA TCTACTTTTC 
TCCTACCAGC CTCCACAGTC CTCTAGCCGA TTTTCAGTTT CGCAGACAGG AGACCTCACC 
ATTACGAACG TCCAGCGGTC TGATGTCGGC TACTACATCT GTCAGACTTT AAATGTTGCT 
GGAAGTATCA TCACAAAGGC CTATTTGGAA GTTACTGATG TGATTGCAGA CCGGCCTCCC 
CCAGTCATTC GGCAAGGTCC TGTGAATCAG ACTGTAGCAG TGGATGGCAC TTTAATCCTC 
AGCTGTGTGG CCACAGGCAG TCCAGCACCC ACAATTCTGT GGAGGAAGGA TGGAGTCCTT 
GTTTCAACCC AAGATTCTCG GATCAAACAG CTGGAGAGTG GCGTGCTGCA GATCCGCTAC 
GCTAAGCTGG GTGACACGGG TCGGTACACC TGCACCGCAT CGACCCCCAG CGGTGAGGCC 
ACGTGGAGCG CTTACATCGA AGTCCAGGAA TTTGGGGTTC CAGTTCAACC CCCAAGACCC 
ACCGACCCCA ACTTAATCCC CAGCGCCCCC TCAAAGCCCG AAGTTACAGA TGTCAGCAAA 
AACACGGTCA CTTTATCGTG GCAGCCAAAC TTGAACTCAG GAGCAACCCC AACATCTTAT 
ATTATAGAAG CCTTCAGCCA TGCATCTGGT AGCAGCTGGC AGACGGCGGC AGAAAATGTG 
AAAACAGAAA CATTTGCCAT CAAAGGACTC AAACCTAACG CCATTTACCT TTTCCTGGTG 
AGGGCAGCAA ATGCCTATGG AATTAGTGAT CCAAGCCAAA TATCAGATCC AGTGAAAACG 
CAAGATGTCC CACCAACCAG CCAAGGGGTG GACCACAAGC AGGTCCAAAG AGAACTGGGG 
AATGTTGTCT TACATCTGCA CAACCCGACC ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC 
TGGACGGTGG ACCAACAATC TCAATATATT CAAGGCTATA AAATTCTCTA CCGCCCATCT 
GGAGCCAGCC ATGGTGAATC GGAGTGGTTA GTTTTTGAAG TGAGGACGCC AACGAAAAAC 
AGCGTGGTGA TCCCCGATCT CAGAAAAGGC GTCAACTACG AGATTAAGGC TCGCCCATTT 
TTTAATGAGT TTCAAGGAGC AGACAGTGAA ATCAAATTTG CCAAAACCTT AGAAGAAGCA 
CCAAGTGCCC CTCCCCGAAG TGTAACTGTA TCCAAGAATG ATGGAAATGG GACTGCAATC 
CTGGTCACTT GGCAACCGCC TCCTGAAGAC ACACAAAATG GAATGGTCCA AGAATATAAG 
GTTTGGTGTC TCGGTAATGA AACGAAGTAT CACATAAACA AGACAGTCGA TGGCTCGACC 
TTCTCGGTGG TCATCCCCTC TCTGGTTCCT GGGATCCGAT ACAGTGTGGA GGTGGCAGCG 
AGCACTGGGG CGGGTCCCGG GGTGAAGAGC GAACCTCAGT TCATCCAGTT AGATTCTCAC 
GGAAACCCCG TGTCTCCCGA GGACCAAGTC AGCCTTGCTC AGCAGATCTC CGACGTGGTG 
AGGCAGCCGG CGTTCATTGC AGGCATTGGG GCAGCCTGTT GGATTATCCT CATGGTCTTC 
AGCATCTGGC TTTACAGACA CCGCAAGAAG AGAAACGGAC TCACCAGCAC GTATGCGGGT 
ATCCGGAAAG TCCCGTCCTT TACCTTCACG CCAACAGTAA CTTATCAAAG AGGAGGCGAA 
GCCGTCAGCA GTGGAGGGAG GCCGGGACTC CTCAACATTA GTGAACCTGC CACGCAGCCA 
TGGCTGGCAG ACACGTGGCC CAACACAGGC AACAACCACA ATGACTGCTC CATCAACTGC 
TGCACGGCCG GCAATGGCAA CAGCGACAGC AACCTGACAA CCTACAGTCG CCCAGCTGAT 
TGTATAGCCA ATTATAACAA CCAACTGGAT AACAAACAAA CAAATCTGAT GCTCCCTGAG 
TCAACTGTTT ATGGTGATGT GGACCTTAGT AACAAAATCA ATGAGATGAA AACCTTCAAT 
AGCCCAAATC TGAAGGACGG GCGTTTTGTC AATCCATCAG GGCAGCCCAC TCCCTATGCC 
ACCACTCAAC TCATCCAGGC GAACCTCAGC AACAATATGA ATAATGGCGC CGGGGACTCC 
AGCGAGAAGC ACTGGAAGCC TCCGGGACAG CAGAAACCGG AAGTGGCACC AATTCAGTAT 
AACATCATGG AGCAAAACAA GCTGAACAAA GATTATCGGG CAAATGACAC GATCCCTCCA 
ACCATCCCAT ACAACCAGTC CTATGACCAG AACACAGGAG GGTCTTATAA CAGCTCAGAT 
CGGGGCAGTA GTACATCCGG GAGTCAAGGC CACAAGAAAG GGGCACGGAC ACCAAAGGCA 
CCCAAGCAGG GTGGCATGAA CTGGGCAGAC TTGCTGCCCC CTCCTCCGGC GCATCCTCCC 
CCACATAGCA ACAGTGAAGA ATACAACATG TCCGTAGATG AAAGCTATGA TCAAGAAATG 
CCCTGTCCAG TGCCACCTGC GCGGATGTAT TTGCAACAGG ATGAATTAGA GGAAGAGGAA 
GATGAAAGAG GCCCCACTCC CCCTGTCCGG GGGGCAGCTT CTTCTCCAGC TGCTGTGTCC 
TACAGCCACC AATCCACGGC CACTCTCACC CCTTCTCCCC AGGAAGAGCT CCAGCCCATG 
TTACAGGATT GTCCGGAGGA CCTCGGGCAC ATGCCCCACC CACCAGACAG GAGGCGGCAG 
CCCGTGAGTC CTCCTCCACC GCCACGGCCA ATTTCCCCAC CTCATACTTA CGGCTACATT 
TCAGGACCCC TTGTCTCAGA TATGGATACC GATGCCCCAG AAGAGGAGGA GGACGAAGCT 
GACATGGAAG TTGCCAAAAT GCAGACCCGA AGGCTGTTGT TACGTGGGCT GGAGCAGACC 
CCAGCATCCA GTGTTGGGGA CCTTGAGAGC TCCGTCACTG GGTCCATGAT CAATGGCTGG 
GGCTCAGCCT CGGAAGAGGA CAACATTTCC AGTGGGCGCT CCAGTGTCAG TTCATCCGAT 
GGCTCCTTTT TCACTGACGC TGATTTTGCC CAGGCAGTTG CTGCAGCTGC GGAGTATGCG 
GGCCTGAAAG TGGCTCGCCG CCAAATGCAA GATGCTGCTG GCCGCCGCCA CTTCCATGCC 
TCTCAGTGCC CAAGGCCCAC GAGTCCTGTG TCCACAGACA GCAACATGAG TGCTGTTGTG 
ATCCAGAAAG CCAGACCCGC CAAGAAGCAG AAACACCAGC CAGGACATCT GCGCAGGGAA 
GCCTACGCAG ATGATCTTCC ACCCCCTCCA GTGCCACCAC CTGCTATAAA ATCGCCCACT 
GTCCAGTCCA AGGCACAGCT GGAGGTACGG CCTGTCATGG TGCCAAAACT CGCGTCTATA 
GAAGCAAGGA CAGATAGATC GTCAGACAGA AAAGGAGGCA GTTACAAGGG GAGAGAAGCT 
CTGGATGGAA GACAAGTCAC TGACCTGCGA ACAAATCCAA GTGACCCCAG AGAGGCACAG 
GAACAGCCAA ATGACGGGAA AGGCCGAGGA ACAAAGGCAG CCAAGCGAGA CCTTCCACCA 
GCAAAGACGC ACCTCGGCCA AGAGGATATT CTTCCGTACT GTAGACCTAC TTTCCCAACG 
TCAAACAACC CTAGAGACCC CAGTTCCTCC AGCTCTATGT CATCAAGAGG ATCAGGGAGC 
CGACAAAGAG AGCAAGCAAA TGTGGGCCGA AGGAATATGG CAGAAATGCA AGTACTTGGG 
GGATTTGAAA GAGGAGATGA GAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Robo1
Entrez Gene ID	19876
Full Name	roundabout guidance receptor 1
Synonyms	AW494633,AW742721,DUTT1,Gm310
General protein information	Preferred Names roundabout guidance receptor 1 Names roundabout homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C3.1

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010242	XP_554155.3
Danio rerio (zebrafish)	robo1	NP_571556.1
Drosophila melanogaster (fruit fly)	robo3	NP_001259866.1
Homo sapiens (human)	ROBO1	NP_002932.1
Pan troglodytes (chimpanzee)	ROBO1	XP_516592.3
Gallus gallus (chicken)	ROBO1	XP_004938403.1
Caenorhabditis elegans (roundworm)	sax-3	NP_001024990.1
Xenopus tropicalis (tropical clawed frog)	robo1	NP_001096171.1
	ROBO1	XP_002802665.1
Canis lupus familiaris (dog)	ROBO1	XP_544814.4
Bos taurus (cattle)	ROBO1	NP_001179817.2
Mus musculus (house mouse)	Robo1	NP_062286.2
Rattus norvegicus (Norway rat)	Robo1	NP_071524.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Robo1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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