ROBO1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017006983.1	XP_016862472.1	roundabout homolog 1 isoform X5
XM_011533978.1	XP_011532280.1	roundabout homolog 1 isoform X2
XM_017006982.1	XP_016862471.1	roundabout homolog 1 isoform X3
NM_002941.4	NP_002932.1	roundabout homolog 1 isoform a precursor
NM_133631.3	NP_598334.2	roundabout homolog 1 isoform b
XM_011533976.1	XP_011532278.1	roundabout homolog 1 isoform X1
XM_011533977.2	XP_011532279.1	roundabout homolog 1 isoform X1
XM_017006983.2	XP_016862472.1	roundabout homolog 1 isoform X5
XM_011533980.1	XP_011532282.1	roundabout homolog 1 isoform X8
NM_001145845.1	NP_001139317.1	roundabout homolog 1 isoform d
XM_006713277.3	XP_006713340.1	roundabout homolog 1 isoform X4
NM_002941.3	NP_002932.1	roundabout homolog 1 isoform a precursor
XM_006713277.2	XP_006713340.1	roundabout homolog 1 isoform X4
XM_017006985.1	XP_016862474.1	roundabout homolog 1 isoform X9
XM_011533979.1	XP_011532281.1	roundabout homolog 1 isoform X6
XM_017006984.1	XP_016862473.1	roundabout homolog 1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu94456	XM_017006982.1 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94457	XM_017006983.2 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94458	XM_017006984.1 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94459	XM_017006985.1 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu14416	NM_133631.3 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu15133	NM_001145845.1 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu18976	NM_002941.4 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu37405	XM_006713277.3 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu60276	XM_011533976.1 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu60276	XM_011533977.2 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu60277	XM_011533978.1 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu60278	XM_011533979.1 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu60279	XM_011533980.1 Latest version!	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu94457	XM_017006983.1	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu18976	NM_002941.3	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu37405	XM_006713277.2	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

ROBO1 ( XM_017006982.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862471.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu94456
Clone ID Related Accession (Same CDS sequence)	XM_017006982.1
Accession Version	XM_017006982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGGC TCCCGTCTTC GTCAGGAAGA TTTTCCACCT 
CGCATTGTTG AACACCCTTC AGACCTGATT GTCTCAAAAG GAGAACCTGC AACTTTGAAC 
TGCAAAGCTG AAGGCCGCCC CACACCCACT ATTGAATGGT ACAAAGGGGG AGAGAGAGTG 
GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCGAGTGG ATCTTTATTT 
TTCTTACGTA TAGTACATGG ACGGAAAAGT AGACCTGATG AAGGAGTCTA TGTCTGTGTA 
GCAAGGAATT ACCTTGGAGA GGCTGTGAGC CACAATGCAT CGCTGGAAGT AGCCATACTT 
CGGGATGACT TCAGACAAAA CCCTTCGGAT GTCATGGTTG CAGTAGGAGA GCCTGCAGTA 
ATGGAATGCC AACCTCCACG AGGCCATCCT GAGCCCACCA TTTCATGGAA GAAAGATGGC 
TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GAGGAAAGCT CATGATCACT 
TACACCCGTA AAAGTGACGC TGGCAAATAT GTTTGTGTTG GTACCAATAT GGTTGGGGAA 
CGTGAGAGTG AAGTAGCCGA GCTGACTGTC TTAGAGAGAC CATCATTTGT GAAGAGACCC 
AGTAACTTGG CAGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC CCGAGGTGAC 
CCTGTACCTA CAGTACGATG GAGGAAAGAT GATGGAGAGC TGCCCAAATC CAGATATGAA 
ATCCGAGATG ATCATACCTT GAAAATTAGG AAGGTGACAG CTGGTGACAT GGGTTCATAC 
ACTTGTGTTG CAGAAAATAT GGTGGGCAAA GCTGAAGCAT CTGCTACTCT GACTGTTCAA 
GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCCCGTG ACCAGGTTGT TGCTTTGGGA 
CGGACTGTAA CTTTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTCTGGAGG 
AGAGAAGGGA GTCAGAATCT ACTTTTCTCA TATCAACCAC CACAGTCATC CAGCCGATTT 
TCAGTCTCCC AGACTGGCGA CCTCACAATT ACTAATGTCC AGCGATCTGA TGTTGGTTAT 
TACATCTGCC AGACTTTAAA TGTTGCTGGA AGCATCATCA CAAAGGCATA TTTGGAAGTT 
ACAGATGTGA TTGCAGATCG GCCTCCCCCA GTTATTCGAC AAGGTCCTGT GAATCAGACT 
GTAGCCGTGG ATGGCACTTT CGTCCTCAGC TGTGTGGCCA CAGGCAGTCC AGTGCCCACC 
ATTCTGTGGA GAAAGGATGG AGTCCTCGTT TCAACCCAAG ACTCTCGAAT CAAACAGTTG 
GAGAATGGAG TACTGCAGAT CCGATATGCT AAGCTGGGTG ATACTGGTCG GTACACCTGC 
ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT TCAAGAATTT 
GGAGTTCCAG TTCAGCCTCC AAGACCTACT GACCCAAATT TAATCCCTAG TGCCCCATCA 
AAACCTGAAG TGACAGATGT CAGCAGAAAT ACAGTCACAT TATCGTGGCA ACCAAATTTG 
AATTCAGGAG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC 
AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT CTGCCATTAA AGGACTCAAA 
CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCTAATG CATATGGAAT TAGTGATCCA 
AGCCAAATAT CAGATCCAGT GAAAACACAA GATGTCCTAC CAACAAGTCA GGGGGTGGAC 
CACAAGCAGG TCCAGAGAGA GCTGGGAAAT GCTGTTCTGC ACCTCCACAA CCCCACCGTC 
CTTTCTTCCT CTTCCATCGA AGTGCACTGG ACAGTAGATC AACAGTCTCA GTATATACAA 
GGATATAAAA TTCTCTATCG GCCATCTGGA GCCAACCACG GAGAATCAGA CTGGTTAGTT 
TTTGAAGTGA GGACGCCAGC CAAAAACAGT GTGGTAATCC CTGATCTCAG AAAGGGAGTC 
AACTATGAAA TTAAGGCTCG CCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATC 
AAGTTTGCCA AAACCCTGGA AGAAGCACCC AGTGCCCCAC CCCAAGGTGT AACTGTATCC 
AAGAATGATG GAAACGGAAC TGCAATTCTA GTTAGTTGGC AGCCACCTCC AGAAGACACT 
CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGATACCAC 
ATCAACAAAA CAGTGGATGG TTCCACCTTT TCCGTGGTCA TTCCCTTTCT TGTTCCTGGA 
ATCCGATACA GTGTGGAAGT GGCAGCCAGC ACTGGGGCTG GGTCTGGGGT AAAGAGTGAG 
CCTCAGTTCA TCCAGCTGGA TGCCCATGGA AACCCTGTGT CACCTGAGGA CCAAGTCAGC 
CTCGCTCAGC AGATTTCAGA TGTGGTGAAG CAGCCGGCCT TCATAGCAGG TATTGGAGCA 
GCCTGTTGGA TCATCCTCAT GGTCTTCAGC ATCTGGCTTT ATCGACACCG CAAGAAGAGA 
AACGGACTTA CTAGTACCTA CGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA 
ACAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC TGGACTTCTC 
AACATCAGTG AACCTGCCGC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCTACTCC TTACGCCACC ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGCGG GGACTCTGGC GAGAAGCACT GGAAACCACT GGGACAGCAG 
AAACAAGAAG TGGCACCAGT TCAGTACAAC ATCGTGGAGC AAAACAAGCT GAACAAAGAT 
TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA CGACCAGAAC 
ACAGGAGGAT CCTACAACAG CTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAGGGGCAC 
AAGAAAGGGG CAAGAACACC CAAGGTACCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCTCCA CACAGCAATA GCGAAGAGTA CAACATTTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG 
CAACAAGATG AATTAGAAGA GGAGGAAGAT GAACGAGGCC CCACTCCCCC TGTTCGGGGA 
GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT AGCCATCAGT CCACTGCCAC TCTGACTCCC 
TCCCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CAGAGGAGAC TGGCCACATG 
CAGCACCAGC CCGACAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC ACGGCCGATC 
TCCCCTCCAC ATACCTATGG CTACATTTCA GGACCCCTGG TCTCAGATAT GGATACGGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAGGTAG CCAAGATGCA AACCAGAAGG 
CTTTTGTTAC GTGGGCTTGA GCAGACACCT GCCTCCAGTG TTGGGGACCT GGAGAGCTCT 
GTCACGGGGT CCATGATCAA CGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GTGTTAGTTC TTCGGACGGC TCCTTTTTCA CTGATGCTGA CTTTGCCCAG 
GCAGTCGCAG CAGCGGCAGA GTATGCTGGT CTGAAAGTAG CACGACGGCA AATGCAGGAT 
GCTGCTGGCC GTCGACATTT TCATGCGTCT CAGTGCCCTA GGCCCACAAG TCCCGTGTCT 
ACAGACAGCA ACATGAGTGC CGCCGTAATG CAGAAAACCA GACCAGCCAA GAAACTGAAA 
CACCAGCCAG GACATCTGCG CAGAGAAACC TACACAGATG ATCTTCCACC ACCTCCTGTG 
CCGCCACCTG CTATAAAGTC ACCTACTGCC CAATCCAAGA CACAGCTGGA AGTACGACCT 
GTAGTGGTGC CAAAACTCCC TTCTATGGAT GCAAGAACAG ACAGATCATC AGACAGAAAA 
GGAAGCAGTT ACAAGGGGAG AGAAGTGTTG GATGGAAGAC AGGTTGTTGA CATGCGAACA 
AATCCAGGTG ATCCCAGAGA AGCACAGGAA CAGCAAAATG ACGGGAAAGG ACGTGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTATTGTA GACCTACTTT TCCAACATCA AATAATCCCA GAGATCCCAG TTCCTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT ACTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862471.1
CDS	294..5174
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_017006982.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006982.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGGC TCCCGTCTTC GTCAGGAAGA TTTTCCACCT 
CGCATTGTTG AACACCCTTC AGACCTGATT GTCTCAAAAG GAGAACCTGC AACTTTGAAC 
TGCAAAGCTG AAGGCCGCCC CACACCCACT ATTGAATGGT ACAAAGGGGG AGAGAGAGTG 
GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCGAGTGG ATCTTTATTT 
TTCTTACGTA TAGTACATGG ACGGAAAAGT AGACCTGATG AAGGAGTCTA TGTCTGTGTA 
GCAAGGAATT ACCTTGGAGA GGCTGTGAGC CACAATGCAT CGCTGGAAGT AGCCATACTT 
CGGGATGACT TCAGACAAAA CCCTTCGGAT GTCATGGTTG CAGTAGGAGA GCCTGCAGTA 
ATGGAATGCC AACCTCCACG AGGCCATCCT GAGCCCACCA TTTCATGGAA GAAAGATGGC 
TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GAGGAAAGCT CATGATCACT 
TACACCCGTA AAAGTGACGC TGGCAAATAT GTTTGTGTTG GTACCAATAT GGTTGGGGAA 
CGTGAGAGTG AAGTAGCCGA GCTGACTGTC TTAGAGAGAC CATCATTTGT GAAGAGACCC 
AGTAACTTGG CAGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC CCGAGGTGAC 
CCTGTACCTA CAGTACGATG GAGGAAAGAT GATGGAGAGC TGCCCAAATC CAGATATGAA 
ATCCGAGATG ATCATACCTT GAAAATTAGG AAGGTGACAG CTGGTGACAT GGGTTCATAC 
ACTTGTGTTG CAGAAAATAT GGTGGGCAAA GCTGAAGCAT CTGCTACTCT GACTGTTCAA 
GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCCCGTG ACCAGGTTGT TGCTTTGGGA 
CGGACTGTAA CTTTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTCTGGAGG 
AGAGAAGGGA GTCAGAATCT ACTTTTCTCA TATCAACCAC CACAGTCATC CAGCCGATTT 
TCAGTCTCCC AGACTGGCGA CCTCACAATT ACTAATGTCC AGCGATCTGA TGTTGGTTAT 
TACATCTGCC AGACTTTAAA TGTTGCTGGA AGCATCATCA CAAAGGCATA TTTGGAAGTT 
ACAGATGTGA TTGCAGATCG GCCTCCCCCA GTTATTCGAC AAGGTCCTGT GAATCAGACT 
GTAGCCGTGG ATGGCACTTT CGTCCTCAGC TGTGTGGCCA CAGGCAGTCC AGTGCCCACC 
ATTCTGTGGA GAAAGGATGG AGTCCTCGTT TCAACCCAAG ACTCTCGAAT CAAACAGTTG 
GAGAATGGAG TACTGCAGAT CCGATATGCT AAGCTGGGTG ATACTGGTCG GTACACCTGC 
ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT TCAAGAATTT 
GGAGTTCCAG TTCAGCCTCC AAGACCTACT GACCCAAATT TAATCCCTAG TGCCCCATCA 
AAACCTGAAG TGACAGATGT CAGCAGAAAT ACAGTCACAT TATCGTGGCA ACCAAATTTG 
AATTCAGGAG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC 
AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT CTGCCATTAA AGGACTCAAA 
CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCTAATG CATATGGAAT TAGTGATCCA 
AGCCAAATAT CAGATCCAGT GAAAACACAA GATGTCCTAC CAACAAGTCA GGGGGTGGAC 
CACAAGCAGG TCCAGAGAGA GCTGGGAAAT GCTGTTCTGC ACCTCCACAA CCCCACCGTC 
CTTTCTTCCT CTTCCATCGA AGTGCACTGG ACAGTAGATC AACAGTCTCA GTATATACAA 
GGATATAAAA TTCTCTATCG GCCATCTGGA GCCAACCACG GAGAATCAGA CTGGTTAGTT 
TTTGAAGTGA GGACGCCAGC CAAAAACAGT GTGGTAATCC CTGATCTCAG AAAGGGAGTC 
AACTATGAAA TTAAGGCTCG CCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATC 
AAGTTTGCCA AAACCCTGGA AGAAGCACCC AGTGCCCCAC CCCAAGGTGT AACTGTATCC 
AAGAATGATG GAAACGGAAC TGCAATTCTA GTTAGTTGGC AGCCACCTCC AGAAGACACT 
CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGATACCAC 
ATCAACAAAA CAGTGGATGG TTCCACCTTT TCCGTGGTCA TTCCCTTTCT TGTTCCTGGA 
ATCCGATACA GTGTGGAAGT GGCAGCCAGC ACTGGGGCTG GGTCTGGGGT AAAGAGTGAG 
CCTCAGTTCA TCCAGCTGGA TGCCCATGGA AACCCTGTGT CACCTGAGGA CCAAGTCAGC 
CTCGCTCAGC AGATTTCAGA TGTGGTGAAG CAGCCGGCCT TCATAGCAGG TATTGGAGCA 
GCCTGTTGGA TCATCCTCAT GGTCTTCAGC ATCTGGCTTT ATCGACACCG CAAGAAGAGA 
AACGGACTTA CTAGTACCTA CGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA 
ACAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC TGGACTTCTC 
AACATCAGTG AACCTGCCGC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCTACTCC TTACGCCACC ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGCGG GGACTCTGGC GAGAAGCACT GGAAACCACT GGGACAGCAG 
AAACAAGAAG TGGCACCAGT TCAGTACAAC ATCGTGGAGC AAAACAAGCT GAACAAAGAT 
TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA CGACCAGAAC 
ACAGGAGGAT CCTACAACAG CTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAGGGGCAC 
AAGAAAGGGG CAAGAACACC CAAGGTACCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCTCCA CACAGCAATA GCGAAGAGTA CAACATTTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG 
CAACAAGATG AATTAGAAGA GGAGGAAGAT GAACGAGGCC CCACTCCCCC TGTTCGGGGA 
GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT AGCCATCAGT CCACTGCCAC TCTGACTCCC 
TCCCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CAGAGGAGAC TGGCCACATG 
CAGCACCAGC CCGACAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC ACGGCCGATC 
TCCCCTCCAC ATACCTATGG CTACATTTCA GGACCCCTGG TCTCAGATAT GGATACGGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAGGTAG CCAAGATGCA AACCAGAAGG 
CTTTTGTTAC GTGGGCTTGA GCAGACACCT GCCTCCAGTG TTGGGGACCT GGAGAGCTCT 
GTCACGGGGT CCATGATCAA CGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GTGTTAGTTC TTCGGACGGC TCCTTTTTCA CTGATGCTGA CTTTGCCCAG 
GCAGTCGCAG CAGCGGCAGA GTATGCTGGT CTGAAAGTAG CACGACGGCA AATGCAGGAT 
GCTGCTGGCC GTCGACATTT TCATGCGTCT CAGTGCCCTA GGCCCACAAG TCCCGTGTCT 
ACAGACAGCA ACATGAGTGC CGCCGTAATG CAGAAAACCA GACCAGCCAA GAAACTGAAA 
CACCAGCCAG GACATCTGCG CAGAGAAACC TACACAGATG ATCTTCCACC ACCTCCTGTG 
CCGCCACCTG CTATAAAGTC ACCTACTGCC CAATCCAAGA CACAGCTGGA AGTACGACCT 
GTAGTGGTGC CAAAACTCCC TTCTATGGAT GCAAGAACAG ACAGATCATC AGACAGAAAA 
GGAAGCAGTT ACAAGGGGAG AGAAGTGTTG GATGGAAGAC AGGTTGTTGA CATGCGAACA 
AATCCAGGTG ATCCCAGAGA AGCACAGGAA CAGCAAAATG ACGGGAAAGG ACGTGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTATTGTA GACCTACTTT TCCAACATCA AATAATCCCA GAGATCCCAG TTCCTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT ACTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_017006983.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862472.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu94457
Clone ID Related Accession (Same CDS sequence)	XM_017006983.1 , XM_017006983.2
Accession Version	XM_017006983.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGACG GACTGTAACT TTTCAGTGTG AAGCAACCGG AAATCCTCAA 
CCAGCTATTT TCTGGAGGAG AGAAGGGAGT CAGAATCTAC TTTTCTCATA TCAACCACCA 
CAGTCATCCA GCCGATTTTC AGTCTCCCAG ACTGGCGACC TCACAATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA CATCTGCCAG ACTTTAAATG TTGCTGGAAG CATCATCACA 
AAGGCATATT TGGAAGTTAC AGATGTGATT GCAGATCGGC CTCCCCCAGT TATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCCGTGGAT GGCACTTTCG TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACCAT TCTGTGGAGA AAGGATGGAG TCCTCGTTTC AACCCAAGAC 
TCTCGAATCA AACAGTTGGA GAATGGAGTA CTGCAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AAGCAACATG GAGTGCTTAC 
ATTGAAGTTC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCTACTGA CCCAAATTTA 
ATCCCTAGTG CCCCATCAAA ACCTGAAGTG ACAGATGTCA GCAGAAATAC AGTCACATTA 
TCGTGGCAAC CAAATTTGAA TTCAGGAGCA ACTCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC AGAAACATCT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCTAATGCA 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACACAAGA TGTCCTACCA 
ACAAGTCAGG GGGTGGACCA CAAGCAGGTC CAGAGAGAGC TGGGAAATGC TGTTCTGCAC 
CTCCACAACC CCACCGTCCT TTCTTCCTCT TCCATCGAAG TGCACTGGAC AGTAGATCAA 
CAGTCTCAGT ATATACAAGG ATATAAAATT CTCTATCGGC CATCTGGAGC CAACCACGGA 
GAATCAGACT GGTTAGTTTT TGAAGTGAGG ACGCCAGCCA AAAACAGTGT GGTAATCCCT 
GATCTCAGAA AGGGAGTCAA CTATGAAATT AAGGCTCGCC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATCAA GTTTGCCAAA ACCCTGGAAG AAGCACCCAG TGCCCCACCC 
CAAGGTGTAA CTGTATCCAA GAATGATGGA AACGGAACTG CAATTCTAGT TAGTTGGCAG 
CCACCTCCAG AAGACACTCA AAATGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTC GATACCACAT CAACAAAACA GTGGATGGTT CCACCTTTTC CGTGGTCATT 
CCCTTTCTTG TTCCTGGAAT CCGATACAGT GTGGAAGTGG CAGCCAGCAC TGGGGCTGGG 
TCTGGGGTAA AGAGTGAGCC TCAGTTCATC CAGCTGGATG CCCATGGAAA CCCTGTGTCA 
CCTGAGGACC AAGTCAGCCT CGCTCAGCAG ATTTCAGATG TGGTGAAGCA GCCGGCCTTC 
ATAGCAGGTA TTGGAGCAGC CTGTTGGATC ATCCTCATGG TCTTCAGCAT CTGGCTTTAT 
CGACACCGCA AGAAGAGAAA CGGACTTACT AGTACCTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAC CAGAGAGGAG GCGAAGCTGT CAGCAGTGGA 
GGGAGGCCTG GACTTCTCAA CATCAGTGAA CCTGCCGCGC AGCCATGGCT GGCAGACACG 
TGGCCTAATA CTGGCAACAA CCACAATGAC TGCTCCATCA GCTGCTGCAC GGCAGGCAAT 
GGAAACAGCG ACAGCAACCT CACTACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCTACTCCTT ACGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CGTGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGAGCAAAT GACACAGTTC CTCCAACTAT CCCATACAAC 
CAATCATACG ACCAGAACAC AGGAGGATCC TACAACAGCT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AGGGGCACAA GAAAGGGGCA AGAACACCCA AGGTACCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCTCCACA CAGCAATAGC 
GAAGAGTACA ACATTTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG TCCCGTGCCA 
CCAGCAAGGA TGTATTTGCA ACAAGATGAA TTAGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACTCCCCCTG TTCGGGGAGC AGCTTCTTCT CCAGCTGCCG TGTCCTATAG CCATCAGTCC 
ACTGCCACTC TGACTCCCTC CCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCA 
GAGGAGACTG GCCACATGCA GCACCAGCCC GACAGGAGAC GGCAGCCTGT GAGTCCTCCT 
CCACCACCAC GGCCGATCTC CCCTCCACAT ACCTATGGCT ACATTTCAGG ACCCCTGGTC 
TCAGATATGG ATACGGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAGGTAGCC 
AAGATGCAAA CCAGAAGGCT TTTGTTACGT GGGCTTGAGC AGACACCTGC CTCCAGTGTT 
GGGGACCTGG AGAGCTCTGT CACGGGGTCC ATGATCAACG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGT GTTAGTTCTT CGGACGGCTC CTTTTTCACT 
GATGCTGACT TTGCCCAGGC AGTCGCAGCA GCGGCAGAGT ATGCTGGTCT GAAAGTAGCA 
CGACGGCAAA TGCAGGATGC TGCTGGCCGT CGACATTTTC ATGCGTCTCA GTGCCCTAGG 
CCCACAAGTC CCGTGTCTAC AGACAGCAAC ATGAGTGCCG CCGTAATGCA GAAAACCAGA 
CCAGCCAAGA AACTGAAACA CCAGCCAGGA CATCTGCGCA GAGAAACCTA CACAGATGAT 
CTTCCACCAC CTCCTGTGCC GCCACCTGCT ATAAAGTCAC CTACTGCCCA ATCCAAGACA 
CAGCTGGAAG TACGACCTGT AGTGGTGCCA AAACTCCCTT CTATGGATGC AAGAACAGAC 
AGATCATCAG ACAGAAAAGG AAGCAGTTAC AAGGGGAGAG AAGTGTTGGA TGGAAGACAG 
GTTGTTGACA TGCGAACAAA TCCAGGTGAT CCCAGAGAAG CACAGGAACA GCAAAATGAC 
GGGAAAGGAC GTGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTATTGTAGA CCTACTTTTC CAACATCAAA TAATCCCAGA 
GATCCCAGTT CCTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTAC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862472.1
CDS	968..5806
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_017006983.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006983.1

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGACG GACTGTAACT TTTCAGTGTG AAGCAACCGG AAATCCTCAA 
CCAGCTATTT TCTGGAGGAG AGAAGGGAGT CAGAATCTAC TTTTCTCATA TCAACCACCA 
CAGTCATCCA GCCGATTTTC AGTCTCCCAG ACTGGCGACC TCACAATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA CATCTGCCAG ACTTTAAATG TTGCTGGAAG CATCATCACA 
AAGGCATATT TGGAAGTTAC AGATGTGATT GCAGATCGGC CTCCCCCAGT TATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCCGTGGAT GGCACTTTCG TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACCAT TCTGTGGAGA AAGGATGGAG TCCTCGTTTC AACCCAAGAC 
TCTCGAATCA AACAGTTGGA GAATGGAGTA CTGCAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AAGCAACATG GAGTGCTTAC 
ATTGAAGTTC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCTACTGA CCCAAATTTA 
ATCCCTAGTG CCCCATCAAA ACCTGAAGTG ACAGATGTCA GCAGAAATAC AGTCACATTA 
TCGTGGCAAC CAAATTTGAA TTCAGGAGCA ACTCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC AGAAACATCT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCTAATGCA 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACACAAGA TGTCCTACCA 
ACAAGTCAGG GGGTGGACCA CAAGCAGGTC CAGAGAGAGC TGGGAAATGC TGTTCTGCAC 
CTCCACAACC CCACCGTCCT TTCTTCCTCT TCCATCGAAG TGCACTGGAC AGTAGATCAA 
CAGTCTCAGT ATATACAAGG ATATAAAATT CTCTATCGGC CATCTGGAGC CAACCACGGA 
GAATCAGACT GGTTAGTTTT TGAAGTGAGG ACGCCAGCCA AAAACAGTGT GGTAATCCCT 
GATCTCAGAA AGGGAGTCAA CTATGAAATT AAGGCTCGCC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATCAA GTTTGCCAAA ACCCTGGAAG AAGCACCCAG TGCCCCACCC 
CAAGGTGTAA CTGTATCCAA GAATGATGGA AACGGAACTG CAATTCTAGT TAGTTGGCAG 
CCACCTCCAG AAGACACTCA AAATGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTC GATACCACAT CAACAAAACA GTGGATGGTT CCACCTTTTC CGTGGTCATT 
CCCTTTCTTG TTCCTGGAAT CCGATACAGT GTGGAAGTGG CAGCCAGCAC TGGGGCTGGG 
TCTGGGGTAA AGAGTGAGCC TCAGTTCATC CAGCTGGATG CCCATGGAAA CCCTGTGTCA 
CCTGAGGACC AAGTCAGCCT CGCTCAGCAG ATTTCAGATG TGGTGAAGCA GCCGGCCTTC 
ATAGCAGGTA TTGGAGCAGC CTGTTGGATC ATCCTCATGG TCTTCAGCAT CTGGCTTTAT 
CGACACCGCA AGAAGAGAAA CGGACTTACT AGTACCTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAC CAGAGAGGAG GCGAAGCTGT CAGCAGTGGA 
GGGAGGCCTG GACTTCTCAA CATCAGTGAA CCTGCCGCGC AGCCATGGCT GGCAGACACG 
TGGCCTAATA CTGGCAACAA CCACAATGAC TGCTCCATCA GCTGCTGCAC GGCAGGCAAT 
GGAAACAGCG ACAGCAACCT CACTACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCTACTCCTT ACGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CGTGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGAGCAAAT GACACAGTTC CTCCAACTAT CCCATACAAC 
CAATCATACG ACCAGAACAC AGGAGGATCC TACAACAGCT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AGGGGCACAA GAAAGGGGCA AGAACACCCA AGGTACCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCTCCACA CAGCAATAGC 
GAAGAGTACA ACATTTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG TCCCGTGCCA 
CCAGCAAGGA TGTATTTGCA ACAAGATGAA TTAGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACTCCCCCTG TTCGGGGAGC AGCTTCTTCT CCAGCTGCCG TGTCCTATAG CCATCAGTCC 
ACTGCCACTC TGACTCCCTC CCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCA 
GAGGAGACTG GCCACATGCA GCACCAGCCC GACAGGAGAC GGCAGCCTGT GAGTCCTCCT 
CCACCACCAC GGCCGATCTC CCCTCCACAT ACCTATGGCT ACATTTCAGG ACCCCTGGTC 
TCAGATATGG ATACGGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAGGTAGCC 
AAGATGCAAA CCAGAAGGCT TTTGTTACGT GGGCTTGAGC AGACACCTGC CTCCAGTGTT 
GGGGACCTGG AGAGCTCTGT CACGGGGTCC ATGATCAACG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGT GTTAGTTCTT CGGACGGCTC CTTTTTCACT 
GATGCTGACT TTGCCCAGGC AGTCGCAGCA GCGGCAGAGT ATGCTGGTCT GAAAGTAGCA 
CGACGGCAAA TGCAGGATGC TGCTGGCCGT CGACATTTTC ATGCGTCTCA GTGCCCTAGG 
CCCACAAGTC CCGTGTCTAC AGACAGCAAC ATGAGTGCCG CCGTAATGCA GAAAACCAGA 
CCAGCCAAGA AACTGAAACA CCAGCCAGGA CATCTGCGCA GAGAAACCTA CACAGATGAT 
CTTCCACCAC CTCCTGTGCC GCCACCTGCT ATAAAGTCAC CTACTGCCCA ATCCAAGACA 
CAGCTGGAAG TACGACCTGT AGTGGTGCCA AAACTCCCTT CTATGGATGC AAGAACAGAC 
AGATCATCAG ACAGAAAAGG AAGCAGTTAC AAGGGGAGAG AAGTGTTGGA TGGAAGACAG 
GTTGTTGACA TGCGAACAAA TCCAGGTGAT CCCAGAGAAG CACAGGAACA GCAAAATGAC 
GGGAAAGGAC GTGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTATTGTAGA CCTACTTTTC CAACATCAAA TAATCCCAGA 
GATCCCAGTT CCTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTAC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_017006983.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862472.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu94457
Clone ID Related Accession (Same CDS sequence)	XM_017006983.1 , XM_017006983.2
Accession Version	XM_017006983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGACG GACTGTAACT TTTCAGTGTG AAGCAACCGG AAATCCTCAA 
CCAGCTATTT TCTGGAGGAG AGAAGGGAGT CAGAATCTAC TTTTCTCATA TCAACCACCA 
CAGTCATCCA GCCGATTTTC AGTCTCCCAG ACTGGCGACC TCACAATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA CATCTGCCAG ACTTTAAATG TTGCTGGAAG CATCATCACA 
AAGGCATATT TGGAAGTTAC AGATGTGATT GCAGATCGGC CTCCCCCAGT TATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCCGTGGAT GGCACTTTCG TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACCAT TCTGTGGAGA AAGGATGGAG TCCTCGTTTC AACCCAAGAC 
TCTCGAATCA AACAGTTGGA GAATGGAGTA CTGCAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AAGCAACATG GAGTGCTTAC 
ATTGAAGTTC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCTACTGA CCCAAATTTA 
ATCCCTAGTG CCCCATCAAA ACCTGAAGTG ACAGATGTCA GCAGAAATAC AGTCACATTA 
TCGTGGCAAC CAAATTTGAA TTCAGGAGCA ACTCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC AGAAACATCT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCTAATGCA 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACACAAGA TGTCCTACCA 
ACAAGTCAGG GGGTGGACCA CAAGCAGGTC CAGAGAGAGC TGGGAAATGC TGTTCTGCAC 
CTCCACAACC CCACCGTCCT TTCTTCCTCT TCCATCGAAG TGCACTGGAC AGTAGATCAA 
CAGTCTCAGT ATATACAAGG ATATAAAATT CTCTATCGGC CATCTGGAGC CAACCACGGA 
GAATCAGACT GGTTAGTTTT TGAAGTGAGG ACGCCAGCCA AAAACAGTGT GGTAATCCCT 
GATCTCAGAA AGGGAGTCAA CTATGAAATT AAGGCTCGCC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATCAA GTTTGCCAAA ACCCTGGAAG AAGCACCCAG TGCCCCACCC 
CAAGGTGTAA CTGTATCCAA GAATGATGGA AACGGAACTG CAATTCTAGT TAGTTGGCAG 
CCACCTCCAG AAGACACTCA AAATGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTC GATACCACAT CAACAAAACA GTGGATGGTT CCACCTTTTC CGTGGTCATT 
CCCTTTCTTG TTCCTGGAAT CCGATACAGT GTGGAAGTGG CAGCCAGCAC TGGGGCTGGG 
TCTGGGGTAA AGAGTGAGCC TCAGTTCATC CAGCTGGATG CCCATGGAAA CCCTGTGTCA 
CCTGAGGACC AAGTCAGCCT CGCTCAGCAG ATTTCAGATG TGGTGAAGCA GCCGGCCTTC 
ATAGCAGGTA TTGGAGCAGC CTGTTGGATC ATCCTCATGG TCTTCAGCAT CTGGCTTTAT 
CGACACCGCA AGAAGAGAAA CGGACTTACT AGTACCTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAC CAGAGAGGAG GCGAAGCTGT CAGCAGTGGA 
GGGAGGCCTG GACTTCTCAA CATCAGTGAA CCTGCCGCGC AGCCATGGCT GGCAGACACG 
TGGCCTAATA CTGGCAACAA CCACAATGAC TGCTCCATCA GCTGCTGCAC GGCAGGCAAT 
GGAAACAGCG ACAGCAACCT CACTACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCTACTCCTT ACGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CGTGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGAGCAAAT GACACAGTTC CTCCAACTAT CCCATACAAC 
CAATCATACG ACCAGAACAC AGGAGGATCC TACAACAGCT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AGGGGCACAA GAAAGGGGCA AGAACACCCA AGGTACCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCTCCACA CAGCAATAGC 
GAAGAGTACA ACATTTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG TCCCGTGCCA 
CCAGCAAGGA TGTATTTGCA ACAAGATGAA TTAGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACTCCCCCTG TTCGGGGAGC AGCTTCTTCT CCAGCTGCCG TGTCCTATAG CCATCAGTCC 
ACTGCCACTC TGACTCCCTC CCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCA 
GAGGAGACTG GCCACATGCA GCACCAGCCC GACAGGAGAC GGCAGCCTGT GAGTCCTCCT 
CCACCACCAC GGCCGATCTC CCCTCCACAT ACCTATGGCT ACATTTCAGG ACCCCTGGTC 
TCAGATATGG ATACGGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAGGTAGCC 
AAGATGCAAA CCAGAAGGCT TTTGTTACGT GGGCTTGAGC AGACACCTGC CTCCAGTGTT 
GGGGACCTGG AGAGCTCTGT CACGGGGTCC ATGATCAACG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGT GTTAGTTCTT CGGACGGCTC CTTTTTCACT 
GATGCTGACT TTGCCCAGGC AGTCGCAGCA GCGGCAGAGT ATGCTGGTCT GAAAGTAGCA 
CGACGGCAAA TGCAGGATGC TGCTGGCCGT CGACATTTTC ATGCGTCTCA GTGCCCTAGG 
CCCACAAGTC CCGTGTCTAC AGACAGCAAC ATGAGTGCCG CCGTAATGCA GAAAACCAGA 
CCAGCCAAGA AACTGAAACA CCAGCCAGGA CATCTGCGCA GAGAAACCTA CACAGATGAT 
CTTCCACCAC CTCCTGTGCC GCCACCTGCT ATAAAGTCAC CTACTGCCCA ATCCAAGACA 
CAGCTGGAAG TACGACCTGT AGTGGTGCCA AAACTCCCTT CTATGGATGC AAGAACAGAC 
AGATCATCAG ACAGAAAAGG AAGCAGTTAC AAGGGGAGAG AAGTGTTGGA TGGAAGACAG 
GTTGTTGACA TGCGAACAAA TCCAGGTGAT CCCAGAGAAG CACAGGAACA GCAAAATGAC 
GGGAAAGGAC GTGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTATTGTAGA CCTACTTTTC CAACATCAAA TAATCCCAGA 
GATCCCAGTT CCTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTAC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862472.1
CDS	1015..5853
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_017006983.2
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006983.2

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGACG GACTGTAACT TTTCAGTGTG AAGCAACCGG AAATCCTCAA 
CCAGCTATTT TCTGGAGGAG AGAAGGGAGT CAGAATCTAC TTTTCTCATA TCAACCACCA 
CAGTCATCCA GCCGATTTTC AGTCTCCCAG ACTGGCGACC TCACAATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA CATCTGCCAG ACTTTAAATG TTGCTGGAAG CATCATCACA 
AAGGCATATT TGGAAGTTAC AGATGTGATT GCAGATCGGC CTCCCCCAGT TATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCCGTGGAT GGCACTTTCG TCCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACCAT TCTGTGGAGA AAGGATGGAG TCCTCGTTTC AACCCAAGAC 
TCTCGAATCA AACAGTTGGA GAATGGAGTA CTGCAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AAGCAACATG GAGTGCTTAC 
ATTGAAGTTC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCTACTGA CCCAAATTTA 
ATCCCTAGTG CCCCATCAAA ACCTGAAGTG ACAGATGTCA GCAGAAATAC AGTCACATTA 
TCGTGGCAAC CAAATTTGAA TTCAGGAGCA ACTCCAACAT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC AGAAACATCT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCTAATGCA 
TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACACAAGA TGTCCTACCA 
ACAAGTCAGG GGGTGGACCA CAAGCAGGTC CAGAGAGAGC TGGGAAATGC TGTTCTGCAC 
CTCCACAACC CCACCGTCCT TTCTTCCTCT TCCATCGAAG TGCACTGGAC AGTAGATCAA 
CAGTCTCAGT ATATACAAGG ATATAAAATT CTCTATCGGC CATCTGGAGC CAACCACGGA 
GAATCAGACT GGTTAGTTTT TGAAGTGAGG ACGCCAGCCA AAAACAGTGT GGTAATCCCT 
GATCTCAGAA AGGGAGTCAA CTATGAAATT AAGGCTCGCC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATCAA GTTTGCCAAA ACCCTGGAAG AAGCACCCAG TGCCCCACCC 
CAAGGTGTAA CTGTATCCAA GAATGATGGA AACGGAACTG CAATTCTAGT TAGTTGGCAG 
CCACCTCCAG AAGACACTCA AAATGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTC GATACCACAT CAACAAAACA GTGGATGGTT CCACCTTTTC CGTGGTCATT 
CCCTTTCTTG TTCCTGGAAT CCGATACAGT GTGGAAGTGG CAGCCAGCAC TGGGGCTGGG 
TCTGGGGTAA AGAGTGAGCC TCAGTTCATC CAGCTGGATG CCCATGGAAA CCCTGTGTCA 
CCTGAGGACC AAGTCAGCCT CGCTCAGCAG ATTTCAGATG TGGTGAAGCA GCCGGCCTTC 
ATAGCAGGTA TTGGAGCAGC CTGTTGGATC ATCCTCATGG TCTTCAGCAT CTGGCTTTAT 
CGACACCGCA AGAAGAGAAA CGGACTTACT AGTACCTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAC CAGAGAGGAG GCGAAGCTGT CAGCAGTGGA 
GGGAGGCCTG GACTTCTCAA CATCAGTGAA CCTGCCGCGC AGCCATGGCT GGCAGACACG 
TGGCCTAATA CTGGCAACAA CCACAATGAC TGCTCCATCA GCTGCTGCAC GGCAGGCAAT 
GGAAACAGCG ACAGCAACCT CACTACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCTACTCCTT ACGCCACCAC TCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGCGGGG ACTCTGGCGA GAAGCACTGG 
AAACCACTGG GACAGCAGAA ACAAGAAGTG GCACCAGTTC AGTACAACAT CGTGGAGCAA 
AACAAGCTGA ACAAAGATTA TCGAGCAAAT GACACAGTTC CTCCAACTAT CCCATACAAC 
CAATCATACG ACCAGAACAC AGGAGGATCC TACAACAGCT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AGGGGCACAA GAAAGGGGCA AGAACACCCA AGGTACCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCTCCACA CAGCAATAGC 
GAAGAGTACA ACATTTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG TCCCGTGCCA 
CCAGCAAGGA TGTATTTGCA ACAAGATGAA TTAGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACTCCCCCTG TTCGGGGAGC AGCTTCTTCT CCAGCTGCCG TGTCCTATAG CCATCAGTCC 
ACTGCCACTC TGACTCCCTC CCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCA 
GAGGAGACTG GCCACATGCA GCACCAGCCC GACAGGAGAC GGCAGCCTGT GAGTCCTCCT 
CCACCACCAC GGCCGATCTC CCCTCCACAT ACCTATGGCT ACATTTCAGG ACCCCTGGTC 
TCAGATATGG ATACGGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAGGTAGCC 
AAGATGCAAA CCAGAAGGCT TTTGTTACGT GGGCTTGAGC AGACACCTGC CTCCAGTGTT 
GGGGACCTGG AGAGCTCTGT CACGGGGTCC ATGATCAACG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGT GTTAGTTCTT CGGACGGCTC CTTTTTCACT 
GATGCTGACT TTGCCCAGGC AGTCGCAGCA GCGGCAGAGT ATGCTGGTCT GAAAGTAGCA 
CGACGGCAAA TGCAGGATGC TGCTGGCCGT CGACATTTTC ATGCGTCTCA GTGCCCTAGG 
CCCACAAGTC CCGTGTCTAC AGACAGCAAC ATGAGTGCCG CCGTAATGCA GAAAACCAGA 
CCAGCCAAGA AACTGAAACA CCAGCCAGGA CATCTGCGCA GAGAAACCTA CACAGATGAT 
CTTCCACCAC CTCCTGTGCC GCCACCTGCT ATAAAGTCAC CTACTGCCCA ATCCAAGACA 
CAGCTGGAAG TACGACCTGT AGTGGTGCCA AAACTCCCTT CTATGGATGC AAGAACAGAC 
AGATCATCAG ACAGAAAAGG AAGCAGTTAC AAGGGGAGAG AAGTGTTGGA TGGAAGACAG 
GTTGTTGACA TGCGAACAAA TCCAGGTGAT CCCAGAGAAG CACAGGAACA GCAAAATGAC 
GGGAAAGGAC GTGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTATTGTAGA CCTACTTTTC CAACATCAAA TAATCCCAGA 
GATCCCAGTT CCTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTAC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_017006984.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862473.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

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CloneID	OHu94458
Clone ID Related Accession (Same CDS sequence)	XM_017006984.1
Accession Version	XM_017006984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTCT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC CGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCG 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATCTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT AATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT TGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACCTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCTGGAC TTCTCAACAT CAGTGAACCT GCCGCGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGGGC AGCCTACTCC TTACGCCACC 
ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC AACATGAACA ATGGCAGCGG GGACTCTGGC 
GAGAAGCACT GGAAACCACT GGGACAGCAG AAACAAGAAG TGGCACCAGT TCAGTACAAC 
ATCGTGGAGC AAAACAAGCT GAACAAAGAT TATCGAGCAA ATGACACAGT TCCTCCAACT 
ATCCCATACA ACCAATCATA CGACCAGAAC ACAGGAGGAT CCTACAACAG CTCAGACCGG 
GGCAGTAGTA CATCTGGGAG TCAGGGGCAC AAGAAAGGGG CAAGAACACC CAAGGTACCA 
AAACAGGGTG GCATGAACTG GGCAGACCTG CTTCCTCCTC CCCCAGCACA TCCTCCTCCA 
CACAGCAATA GCGAAGAGTA CAACATTTCT GTAGATGAAA GCTATGACCA AGAAATGCCA 
TGTCCCGTGC CACCAGCAAG GATGTATTTG CAACAAGATG AATTAGAAGA GGAGGAAGAT 
GAACGAGGCC CCACTCCCCC TGTTCGGGGA GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT 
AGCCATCAGT CCACTGCCAC TCTGACTCCC TCCCCACAGG AAGAACTCCA GCCCATGTTA 
CAGGATTGTC CAGAGGAGAC TGGCCACATG CAGCACCAGC CCGACAGGAG ACGGCAGCCT 
GTGAGTCCTC CTCCACCACC ACGGCCGATC TCCCCTCCAC ATACCTATGG CTACATTTCA 
GGACCCCTGG TCTCAGATAT GGATACGGAT GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC 
ATGGAGGTAG CCAAGATGCA AACCAGAAGG CTTTTGTTAC GTGGGCTTGA GCAGACACCT 
GCCTCCAGTG TTGGGGACCT GGAGAGCTCT GTCACGGGGT CCATGATCAA CGGCTGGGGC 
TCAGCCTCAG AGGAGGACAA CATTTCCAGC GGACGCTCCA GTGTTAGTTC TTCGGACGGC 
TCCTTTTTCA CTGATGCTGA CTTTGCCCAG GCAGTCGCAG CAGCGGCAGA GTATGCTGGT 
CTGAAAGTAG CACGACGGCA AATGCAGGAT GCTGCTGGCC GTCGACATTT TCATGCGTCT 
CAGTGCCCTA GGCCCACAAG TCCCGTGTCT ACAGACAGCA ACATGAGTGC CGCCGTAATG 
CAGAAAACCA GACCAGCCAA GAAACTGAAA CACCAGCCAG GACATCTGCG CAGAGAAACC 
TACACAGATG ATCTTCCACC ACCTCCTGTG CCGCCACCTG CTATAAAGTC ACCTACTGCC 
CAATCCAAGA CACAGCTGGA AGTACGACCT GTAGTGGTGC CAAAACTCCC TTCTATGGAT 
GCAAGAACAG ACAGATCATC AGACAGAAAA GGAAGCAGTT ACAAGGGGAG AGAAGTGTTG 
GATGGAAGAC AGGTTGTTGA CATGCGAACA AATCCAGGTG ATCCCAGAGA AGCACAGGAA 
CAGCAAAATG ACGGGAAAGG ACGTGGAAAC AAGGCAGCAA AACGAGACCT TCCACCAGCA 
AAGACTCATC TCATCCAAGA GGATATTCTA CCTTATTGTA GACCTACTTT TCCAACATCA 
AATAATCCCA GAGATCCCAG TTCCTCAAGC TCAATGTCAT CAAGAGGATC AGGAAGCAGA 
CAAAGAGAAC AAGCAAATGT AGGTCGAAGA AATATTGCAG AAATGCAGGT ACTTGGAGGA 
TATGAAAGAG GAGAAGATAA TAATGAAGAA TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862473.1
CDS	298..5088
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_017006984.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006984.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTCT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC CGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCG 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATCTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT AATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT TGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACCTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCTGGAC TTCTCAACAT CAGTGAACCT GCCGCGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGGGC AGCCTACTCC TTACGCCACC 
ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC AACATGAACA ATGGCAGCGG GGACTCTGGC 
GAGAAGCACT GGAAACCACT GGGACAGCAG AAACAAGAAG TGGCACCAGT TCAGTACAAC 
ATCGTGGAGC AAAACAAGCT GAACAAAGAT TATCGAGCAA ATGACACAGT TCCTCCAACT 
ATCCCATACA ACCAATCATA CGACCAGAAC ACAGGAGGAT CCTACAACAG CTCAGACCGG 
GGCAGTAGTA CATCTGGGAG TCAGGGGCAC AAGAAAGGGG CAAGAACACC CAAGGTACCA 
AAACAGGGTG GCATGAACTG GGCAGACCTG CTTCCTCCTC CCCCAGCACA TCCTCCTCCA 
CACAGCAATA GCGAAGAGTA CAACATTTCT GTAGATGAAA GCTATGACCA AGAAATGCCA 
TGTCCCGTGC CACCAGCAAG GATGTATTTG CAACAAGATG AATTAGAAGA GGAGGAAGAT 
GAACGAGGCC CCACTCCCCC TGTTCGGGGA GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT 
AGCCATCAGT CCACTGCCAC TCTGACTCCC TCCCCACAGG AAGAACTCCA GCCCATGTTA 
CAGGATTGTC CAGAGGAGAC TGGCCACATG CAGCACCAGC CCGACAGGAG ACGGCAGCCT 
GTGAGTCCTC CTCCACCACC ACGGCCGATC TCCCCTCCAC ATACCTATGG CTACATTTCA 
GGACCCCTGG TCTCAGATAT GGATACGGAT GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC 
ATGGAGGTAG CCAAGATGCA AACCAGAAGG CTTTTGTTAC GTGGGCTTGA GCAGACACCT 
GCCTCCAGTG TTGGGGACCT GGAGAGCTCT GTCACGGGGT CCATGATCAA CGGCTGGGGC 
TCAGCCTCAG AGGAGGACAA CATTTCCAGC GGACGCTCCA GTGTTAGTTC TTCGGACGGC 
TCCTTTTTCA CTGATGCTGA CTTTGCCCAG GCAGTCGCAG CAGCGGCAGA GTATGCTGGT 
CTGAAAGTAG CACGACGGCA AATGCAGGAT GCTGCTGGCC GTCGACATTT TCATGCGTCT 
CAGTGCCCTA GGCCCACAAG TCCCGTGTCT ACAGACAGCA ACATGAGTGC CGCCGTAATG 
CAGAAAACCA GACCAGCCAA GAAACTGAAA CACCAGCCAG GACATCTGCG CAGAGAAACC 
TACACAGATG ATCTTCCACC ACCTCCTGTG CCGCCACCTG CTATAAAGTC ACCTACTGCC 
CAATCCAAGA CACAGCTGGA AGTACGACCT GTAGTGGTGC CAAAACTCCC TTCTATGGAT 
GCAAGAACAG ACAGATCATC AGACAGAAAA GGAAGCAGTT ACAAGGGGAG AGAAGTGTTG 
GATGGAAGAC AGGTTGTTGA CATGCGAACA AATCCAGGTG ATCCCAGAGA AGCACAGGAA 
CAGCAAAATG ACGGGAAAGG ACGTGGAAAC AAGGCAGCAA AACGAGACCT TCCACCAGCA 
AAGACTCATC TCATCCAAGA GGATATTCTA CCTTATTGTA GACCTACTTT TCCAACATCA 
AATAATCCCA GAGATCCCAG TTCCTCAAGC TCAATGTCAT CAAGAGGATC AGGAAGCAGA 
CAAAGAGAAC AAGCAAATGT AGGTCGAAGA AATATTGCAG AAATGCAGGT ACTTGGAGGA 
TATGAAAGAG GAGAAGATAA TAATGAAGAA TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_017006985.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862474.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.

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CloneID	OHu94459
Clone ID Related Accession (Same CDS sequence)	XM_017006985.1
Accession Version	XM_017006985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC ATCAGTGAAC CTGCCGCGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA CGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
GTGGAGCAAA ACAAGCTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC 
CCATACAACC AATCATACGA CCAGAACACA GGAGGATCCT ACAACAGCTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA GGGGCACAAG AAAGGGGCAA GAACACCCAA GGTACCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCTCCACAC 
AGCAATAGCG AAGAGTACAA CATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT 
CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAAGATGAAT TAGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CTCCCCCTGT TCGGGGAGCA GCTTCTTCTC CAGCTGCCGT GTCCTATAGC 
CATCAGTCCA CTGCCACTCT GACTCCCTCC CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCAG AGGAGACTGG CCACATGCAG CACCAGCCCG ACAGGAGACG GCAGCCTGTG 
AGTCCTCCTC CACCACCACG GCCGATCTCC CCTCCACATA CCTATGGCTA CATTTCAGGA 
CCCCTGGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAGGTAGCCA AGATGCAAAC CAGAAGGCTT TTGTTACGTG GGCTTGAGCA GACACCTGCC 
TCCAGTGTTG GGGACCTGGA GAGCTCTGTC ACGGGGTCCA TGATCAACGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGTG TTAGTTCTTC GGACGGCTCC 
TTTTTCACTG ATGCTGACTT TGCCCAGGCA GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG 
AAAGTAGCAC GACGGCAAAT GCAGGATGCT GCTGGCCGTC GACATTTTCA TGCGTCTCAG 
TGCCCTAGGC CCACAAGTCC CGTGTCTACA GACAGCAACA TGAGTGCCGC CGTAATGCAG 
AAAACCAGAC CAGCCAAGAA ACTGAAACAC CAGCCAGGAC ATCTGCGCAG AGAAACCTAC 
ACAGATGATC TTCCACCACC TCCTGTGCCG CCACCTGCTA TAAAGTCACC TACTGCCCAA 
TCCAAGACAC AGCTGGAAGT ACGACCTGTA GTGGTGCCAA AACTCCCTTC TATGGATGCA 
AGAACAGACA GATCATCAGA CAGAAAAGGA AGCAGTTACA AGGGGAGAGA AGTGTTGGAT 
GGAAGACAGG TTGTTGACAT GCGAACAAAT CCAGGTGATC CCAGAGAAGC ACAGGAACAG 
CAAAATGACG GGAAAGGACG TGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TATTGTAGAC CTACTTTTCC AACATCAAAT 
AATCCCAGAG ATCCCAGTTC CTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTACT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862474.1
CDS	308..4915
Translation	MLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_017006985.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006985.1

ORF Insert Sequence:

ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC ATCAGTGAAC CTGCCGCGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA CGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
GTGGAGCAAA ACAAGCTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC 
CCATACAACC AATCATACGA CCAGAACACA GGAGGATCCT ACAACAGCTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA GGGGCACAAG AAAGGGGCAA GAACACCCAA GGTACCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCTCCACAC 
AGCAATAGCG AAGAGTACAA CATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT 
CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAAGATGAAT TAGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CTCCCCCTGT TCGGGGAGCA GCTTCTTCTC CAGCTGCCGT GTCCTATAGC 
CATCAGTCCA CTGCCACTCT GACTCCCTCC CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCAG AGGAGACTGG CCACATGCAG CACCAGCCCG ACAGGAGACG GCAGCCTGTG 
AGTCCTCCTC CACCACCACG GCCGATCTCC CCTCCACATA CCTATGGCTA CATTTCAGGA 
CCCCTGGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAGGTAGCCA AGATGCAAAC CAGAAGGCTT TTGTTACGTG GGCTTGAGCA GACACCTGCC 
TCCAGTGTTG GGGACCTGGA GAGCTCTGTC ACGGGGTCCA TGATCAACGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGTG TTAGTTCTTC GGACGGCTCC 
TTTTTCACTG ATGCTGACTT TGCCCAGGCA GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG 
AAAGTAGCAC GACGGCAAAT GCAGGATGCT GCTGGCCGTC GACATTTTCA TGCGTCTCAG 
TGCCCTAGGC CCACAAGTCC CGTGTCTACA GACAGCAACA TGAGTGCCGC CGTAATGCAG 
AAAACCAGAC CAGCCAAGAA ACTGAAACAC CAGCCAGGAC ATCTGCGCAG AGAAACCTAC 
ACAGATGATC TTCCACCACC TCCTGTGCCG CCACCTGCTA TAAAGTCACC TACTGCCCAA 
TCCAAGACAC AGCTGGAAGT ACGACCTGTA GTGGTGCCAA AACTCCCTTC TATGGATGCA 
AGAACAGACA GATCATCAGA CAGAAAAGGA AGCAGTTACA AGGGGAGAGA AGTGTTGGAT 
GGAAGACAGG TTGTTGACAT GCGAACAAAT CCAGGTGATC CCAGAGAAGC ACAGGAACAG 
CAAAATGACG GGAAAGGACG TGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TATTGTAGAC CTACTTTTCC AACATCAAAT 
AATCCCAGAG ATCCCAGTTC CTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTACT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( NM_133631.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_598334.2 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 2, mRNA.

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CloneID	OHu14416
Clone ID Related Accession (Same CDS sequence)	NM_133631.3
Accession Version	NM_133631.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP228659.1, AC016946.15, BC157861.1, BC115020.1 and BC029676.1. On or before Apr 8, 2010 this sequence version replaced NM_001145844.1, NM_133631.2. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, uses a distinct translation start site, includes an alternate in-frame exon in the 5' coding region, and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus, compared to isoform a. The 5' UTR of this variant contains a 55 aa uORF with a strong Kozak signal that may inhibit translation of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC157861.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC TGGACTTCTC 
AACATCAGTG AACCTGCCGC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCTACTCC TTACGCCACC ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGCGG GGACTCTGGC GAGAAGCACT GGAAACCACT GGGACAGCAG 
AAACAAGAAG TGGCACCAGT TCAGTACAAC ATCGTGGAGC AAAACAAGCT GAACAAAGAT 
TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA CGACCAGAAC 
ACAGGAGGAT CCTACAACAG CTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAGGGGCAC 
AAGAAAGGGG CAAGAACACC CAAGGTACCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCTCCA CACAGCAATA GCGAAGAGTA CAACATTTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG 
CAACAAGATG AATTAGAAGA GGAGGAAGAT GAACGAGGCC CCACTCCCCC TGTTCGGGGA 
GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT AGCCATCAGT CCACTGCCAC TCTGACTCCC 
TCCCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CAGAGGAGAC TGGCCACATG 
CAGCACCAGC CCGACAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC ACGGCCGATC 
TCCCCTCCAC ATACCTATGG CTACATTTCA GGACCCCTGG TCTCAGATAT GGATACGGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAGGTAG CCAAGATGCA AACCAGAAGG 
CTTTTGTTAC GTGGGCTTGA GCAGACACCT GCCTCCAGTG TTGGGGACCT GGAGAGCTCT 
GTCACGGGGT CCATGATCAA CGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GTGTTAGTTC TTCGGACGGC TCCTTTTTCA CTGATGCTGA CTTTGCCCAG 
GCAGTCGCAG CAGCGGCAGA GTATGCTGGT CTGAAAGTAG CACGACGGCA AATGCAGGAT 
GCTGCTGGCC GTCGACATTT TCATGCGTCT CAGTGCCCTA GGCCCACAAG TCCCGTGTCT 
ACAGACAGCA ACATGAGTGC CGCCGTAATG CAGAAAACCA GACCAGCCAA GAAACTGAAA 
CACCAGCCAG GACATCTGCG CAGAGAAACC TACACAGATG ATCTTCCACC ACCTCCTGTG 
CCGCCACCTG CTATAAAGTC ACCTACTGCC CAATCCAAGA CACAGCTGGA AGTACGACCT 
GTAGTGGTGC CAAAACTCCC TTCTATGGAT GCAAGAACAG ACAGATCATC AGACAGAAAA 
GGAAGCAGTT ACAAGGGGAG AGAAGTGTTG GATGGAAGAC AGGTTGTTGA CATGCGAACA 
AATCCAGGTG ATCCCAGAGA AGCACAGGAA CAGCAAAATG ACGGGAAAGG ACGTGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTATTGTA GACCTACTTT TCCAACATCA AATAATCCCA GAGATCCCAG TTCCTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT ACTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598334.2
CDS	999..5819
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	NM_133631.3
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133631.3

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC TGGACTTCTC 
AACATCAGTG AACCTGCCGC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCTACTCC TTACGCCACC ACTCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGCGG GGACTCTGGC GAGAAGCACT GGAAACCACT GGGACAGCAG 
AAACAAGAAG TGGCACCAGT TCAGTACAAC ATCGTGGAGC AAAACAAGCT GAACAAAGAT 
TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA CGACCAGAAC 
ACAGGAGGAT CCTACAACAG CTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAGGGGCAC 
AAGAAAGGGG CAAGAACACC CAAGGTACCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCTCCA CACAGCAATA GCGAAGAGTA CAACATTTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG 
CAACAAGATG AATTAGAAGA GGAGGAAGAT GAACGAGGCC CCACTCCCCC TGTTCGGGGA 
GCAGCTTCTT CTCCAGCTGC CGTGTCCTAT AGCCATCAGT CCACTGCCAC TCTGACTCCC 
TCCCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CAGAGGAGAC TGGCCACATG 
CAGCACCAGC CCGACAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC ACGGCCGATC 
TCCCCTCCAC ATACCTATGG CTACATTTCA GGACCCCTGG TCTCAGATAT GGATACGGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAGGTAG CCAAGATGCA AACCAGAAGG 
CTTTTGTTAC GTGGGCTTGA GCAGACACCT GCCTCCAGTG TTGGGGACCT GGAGAGCTCT 
GTCACGGGGT CCATGATCAA CGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GTGTTAGTTC TTCGGACGGC TCCTTTTTCA CTGATGCTGA CTTTGCCCAG 
GCAGTCGCAG CAGCGGCAGA GTATGCTGGT CTGAAAGTAG CACGACGGCA AATGCAGGAT 
GCTGCTGGCC GTCGACATTT TCATGCGTCT CAGTGCCCTA GGCCCACAAG TCCCGTGTCT 
ACAGACAGCA ACATGAGTGC CGCCGTAATG CAGAAAACCA GACCAGCCAA GAAACTGAAA 
CACCAGCCAG GACATCTGCG CAGAGAAACC TACACAGATG ATCTTCCACC ACCTCCTGTG 
CCGCCACCTG CTATAAAGTC ACCTACTGCC CAATCCAAGA CACAGCTGGA AGTACGACCT 
GTAGTGGTGC CAAAACTCCC TTCTATGGAT GCAAGAACAG ACAGATCATC AGACAGAAAA 
GGAAGCAGTT ACAAGGGGAG AGAAGTGTTG GATGGAAGAC AGGTTGTTGA CATGCGAACA 
AATCCAGGTG ATCCCAGAGA AGCACAGGAA CAGCAAAATG ACGGGAAAGG ACGTGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTATTGTA GACCTACTTT TCCAACATCA AATAATCCCA GAGATCCCAG TTCCTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT ACTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( NM_001145845.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139317.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 4, mRNA.

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CloneID	OHu15133
Clone ID Related Accession (Same CDS sequence)	NM_001145845.1
Accession Version	NM_001145845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP228659.1, AC016946.15, BC115022.1, AC055731.22, AC108719.3, AC106720.8, AK025535.1 and BC029676.1. Transcript Variant: This variant (4) represents use of an alternate promoter and 5' UTR, uses a distinct translation start site, includes an alternate in-frame exon in the 5' coding region, lacks an alternate in-frame exon in the central coding region, and uses an alternate splice site in the central coding region, compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus and differs at three internal regions, compared to isoform a. The 5' UTR of this variant contains a 55 aa uORF with a strong Kozak signal that may inhibit translation of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC115022.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC TGGACTTCTC 
AACATCAGTG AACCTGCCGC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGGGCAGCCT ACTCCTTACG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCGT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCTGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ACGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTAGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCCG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC TGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTA TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TGTTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139317.1
CDS	999..5654
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	NM_001145845.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145845.1

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTAACTT ACCAGAGAGG AGGCGAAGCT GTCAGCAGTG GAGGGAGGCC TGGACTTCTC 
AACATCAGTG AACCTGCCGC GCAGCCATGG CTGGCAGACA CGTGGCCTAA TACTGGCAAC 
AACCACAATG ACTGCTCCAT CAGCTGCTGC ACGGCAGGCA ATGGAAACAG CGACAGCAAC 
CTCACTACCT ACAGTCGCCC AGGGCAGCCT ACTCCTTACG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCGT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCTGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ACGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTAGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCCG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC TGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTA TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TGTTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( NM_002941.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002932.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18976
Clone ID Related Accession (Same CDS sequence)	NM_002941.4 , NM_002941.3
Accession Version	NM_002941.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC125624.6, DR003097.1, AF040990.1 and AK025535.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002941.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF040990.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000464233.6/ ENSP00000420321.1 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTCT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC CGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCG 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATCTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT AATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT TGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACCTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCTGGAC TTCTCAACAT CAGTGAACCT GCCGCGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTACG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCGT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCTGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ACGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTAGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCCG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC TGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTA TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TGTTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002932.1
CDS	298..5253
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	NM_002941.4
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002941.4

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTCT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC CGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCG 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATCTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT AATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT TGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACCTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCTGGAC TTCTCAACAT CAGTGAACCT GCCGCGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTACG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCGT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCTGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ACGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTAGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCCG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC TGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTA TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TGTTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( NM_002941.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002932.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 1, mRNA.

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CloneID	OHu18976
Clone ID Related Accession (Same CDS sequence)	NM_002941.4 , NM_002941.3
Accession Version	NM_002941.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR003097.1, AF040990.1, AK025535.1 and BC029676.1. This sequence is a reference standard in the RefSeqGene project. On Mar 14, 2009 this sequence version replaced NM_002941.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF040990.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTCT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC CGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCG 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATCTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT AATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT TGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACCTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCTGGAC TTCTCAACAT CAGTGAACCT GCCGCGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTACG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCGT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCTGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ACGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTAGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCCG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC TGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTA TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TGTTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002932.1
CDS	209..5164
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	NM_002941.3
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002941.3

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA 
GCTATTTTCT GGAGGAGAGA AGGGAGTCAG AATCTACTTT TCTCATATCA ACCACCACAG 
TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA CAATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG CTGGAAGCAT CATCACAAAG 
GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC CCCCAGTTAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC CGTGGATGGC ACTTTCGTCC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC TCGTTTCAAC CCAAGACTCT 
CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT 
GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CTACTGACCC AAATTTAATC 
CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA GAAATACAGT CACATTATCG 
TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATCTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC TAATGCATAT 
GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA CACAAGATGT CCTACCAACA 
AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG GAAATGCTGT TCTGCACCTC 
CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC ACTGGACAGT AGATCAACAG 
TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT CTGGAGCCAA CCACGGAGAA 
TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA ACAGTGTGGT AATCCCTGAT 
CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATCAAGTT TGCCAAAACC CTGGAAGAAG CACCCAGTGC CCCACCCCAA 
GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA TTCTAGTTAG TTGGCAGCCA 
CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA CCTTTTCCGT GGTCATTCCC 
TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG CCAGCACTGG GGCTGGGTCT 
GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC ATGGAAACCC TGTGTCACCT 
GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG TGAAGCAGCC GGCCTTCATA 
GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT TCAGCATCTG GCTTTATCGA 
CACCGCAAGA AGAGAAACGG ACTTACTAGT ACCTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTACCAG AGAGGAGGCG AAGCTGTCAG CAGTGGAGGG 
AGGCCTGGAC TTCTCAACAT CAGTGAACCT GCCGCGCAGC CATGGCTGGC AGACACGTGG 
CCTAATACTG GCAACAACCA CAATGACTGC TCCATCAGCT GCTGCACGGC AGGCAATGGA 
AACAGCGACA GCAACCTCAC TACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCT ACTCCTTACG CCACCACTCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CTGGCGAGAA GCACTGGAAA 
CCACTGGGAC AGCAGAAACA AGAAGTGGCA CCAGTTCAGT ACAACATCGT GGAGCAAAAC 
AAGCTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA 
TCATACGACC AGAACACAGG AGGATCCTAC AACAGCTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAGG GGCACAAGAA AGGGGCAAGA ACACCCAAGG TACCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CTCCACACAG CAATAGCGAA 
GAGTACAACA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA 
GCAAGGATGT ATTTGCAACA AGATGAATTA GAAGAGGAGG AAGATGAACG AGGCCCCACT 
CCCCCTGTTC GGGGAGCAGC TTCTTCTCCA GCTGCCGTGT CCTATAGCCA TCAGTCCACT 
GCCACTCTGA CTCCCTCCCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCAGAG 
GAGACTGGCC ACATGCAGCA CCAGCCCGAC AGGAGACGGC AGCCTGTGAG TCCTCCTCCA 
CCACCACGGC CGATCTCCCC TCCACATACC TATGGCTACA TTTCAGGACC CCTGGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA GGTAGCCAAG 
ATGCAAACCA GAAGGCTTTT GTTACGTGGG CTTGAGCAGA CACCTGCCTC CAGTGTTGGG 
GACCTGGAGA GCTCTGTCAC GGGGTCCATG ATCAACGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGTGTT AGTTCTTCGG ACGGCTCCTT TTTCACTGAT 
GCTGACTTTG CCCAGGCAGT CGCAGCAGCG GCAGAGTATG CTGGTCTGAA AGTAGCACGA 
CGGCAAATGC AGGATGCTGC TGGCCGTCGA CATTTTCATG CGTCTCAGTG CCCTAGGCCC 
ACAAGTCCCG TGTCTACAGA CAGCAACATG AGTGCCGCCG TAATGCAGAA AACCAGACCA 
GCCAAGAAAC TGAAACACCA GCCAGGACAT CTGCGCAGAG AAACCTACAC AGATGATCTT 
CCACCACCTC CTGTGCCGCC ACCTGCTATA AAGTCACCTA CTGCCCAATC CAAGACACAG 
CTGGAAGTAC GACCTGTAGT GGTGCCAAAA CTCCCTTCTA TGGATGCAAG AACAGACAGA 
TCATCAGACA GAAAAGGAAG CAGTTACAAG GGGAGAGAAG TGTTGGATGG AAGACAGGTT 
GTTGACATGC GAACAAATCC AGGTGATCCC AGAGAAGCAC AGGAACAGCA AAATGACGGG 
AAAGGACGTG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA TTGTAGACCT ACTTTTCCAA CATCAAATAA TCCCAGAGAT 
CCCAGTTCCT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTACTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_006713277.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713340.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

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CloneID	OHu37405
Clone ID Related Accession (Same CDS sequence)	XM_006713277.3 , XM_006713277.2
Accession Version	XM_006713277.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006713277.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC ATCAGTGAAC CTGCCGCGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA CGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
GTGGAGCAAA ACAAGCTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC 
CCATACAACC AATCATACGA CCAGAACACA GGAGGATCCT ACAACAGCTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA GGGGCACAAG AAAGGGGCAA GAACACCCAA GGTACCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCTCCACAC 
AGCAATAGCG AAGAGTACAA CATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT 
CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAAGATGAAT TAGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CTCCCCCTGT TCGGGGAGCA GCTTCTTCTC CAGCTGCCGT GTCCTATAGC 
CATCAGTCCA CTGCCACTCT GACTCCCTCC CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCAG AGGAGACTGG CCACATGCAG CACCAGCCCG ACAGGAGACG GCAGCCTGTG 
AGTCCTCCTC CACCACCACG GCCGATCTCC CCTCCACATA CCTATGGCTA CATTTCAGGA 
CCCCTGGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAGGTAGCCA AGATGCAAAC CAGAAGGCTT TTGTTACGTG GGCTTGAGCA GACACCTGCC 
TCCAGTGTTG GGGACCTGGA GAGCTCTGTC ACGGGGTCCA TGATCAACGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGTG TTAGTTCTTC GGACGGCTCC 
TTTTTCACTG ATGCTGACTT TGCCCAGGCA GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG 
AAAGTAGCAC GACGGCAAAT GCAGGATGCT GCTGGCCGTC GACATTTTCA TGCGTCTCAG 
TGCCCTAGGC CCACAAGTCC CGTGTCTACA GACAGCAACA TGAGTGCCGC CGTAATGCAG 
AAAACCAGAC CAGCCAAGAA ACTGAAACAC CAGCCAGGAC ATCTGCGCAG AGAAACCTAC 
ACAGATGATC TTCCACCACC TCCTGTGCCG CCACCTGCTA TAAAGTCACC TACTGCCCAA 
TCCAAGACAC AGCTGGAAGT ACGACCTGTA GTGGTGCCAA AACTCCCTTC TATGGATGCA 
AGAACAGACA GATCATCAGA CAGAAAAGGA AGCAGTTACA AGGGGAGAGA AGTGTTGGAT 
GGAAGACAGG TTGTTGACAT GCGAACAAAT CCAGGTGATC CCAGAGAAGC ACAGGAACAG 
CAAAATGACG GGAAAGGACG TGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TATTGTAGAC CTACTTTTCC AACATCAAAT 
AATCCCAGAG ATCCCAGTTC CTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTACT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713340.1
CDS	1027..5874
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_006713277.3
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713277.3

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC ATCAGTGAAC CTGCCGCGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA CGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
GTGGAGCAAA ACAAGCTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC 
CCATACAACC AATCATACGA CCAGAACACA GGAGGATCCT ACAACAGCTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA GGGGCACAAG AAAGGGGCAA GAACACCCAA GGTACCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCTCCACAC 
AGCAATAGCG AAGAGTACAA CATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT 
CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAAGATGAAT TAGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CTCCCCCTGT TCGGGGAGCA GCTTCTTCTC CAGCTGCCGT GTCCTATAGC 
CATCAGTCCA CTGCCACTCT GACTCCCTCC CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCAG AGGAGACTGG CCACATGCAG CACCAGCCCG ACAGGAGACG GCAGCCTGTG 
AGTCCTCCTC CACCACCACG GCCGATCTCC CCTCCACATA CCTATGGCTA CATTTCAGGA 
CCCCTGGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAGGTAGCCA AGATGCAAAC CAGAAGGCTT TTGTTACGTG GGCTTGAGCA GACACCTGCC 
TCCAGTGTTG GGGACCTGGA GAGCTCTGTC ACGGGGTCCA TGATCAACGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGTG TTAGTTCTTC GGACGGCTCC 
TTTTTCACTG ATGCTGACTT TGCCCAGGCA GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG 
AAAGTAGCAC GACGGCAAAT GCAGGATGCT GCTGGCCGTC GACATTTTCA TGCGTCTCAG 
TGCCCTAGGC CCACAAGTCC CGTGTCTACA GACAGCAACA TGAGTGCCGC CGTAATGCAG 
AAAACCAGAC CAGCCAAGAA ACTGAAACAC CAGCCAGGAC ATCTGCGCAG AGAAACCTAC 
ACAGATGATC TTCCACCACC TCCTGTGCCG CCACCTGCTA TAAAGTCACC TACTGCCCAA 
TCCAAGACAC AGCTGGAAGT ACGACCTGTA GTGGTGCCAA AACTCCCTTC TATGGATGCA 
AGAACAGACA GATCATCAGA CAGAAAAGGA AGCAGTTACA AGGGGAGAGA AGTGTTGGAT 
GGAAGACAGG TTGTTGACAT GCGAACAAAT CCAGGTGATC CCAGAGAAGC ACAGGAACAG 
CAAAATGACG GGAAAGGACG TGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TATTGTAGAC CTACTTTTCC AACATCAAAT 
AATCCCAGAG ATCCCAGTTC CTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTACT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_006713277.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713340.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

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CloneID	OHu37405
Clone ID Related Accession (Same CDS sequence)	XM_006713277.3 , XM_006713277.2
Accession Version	XM_006713277.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006713277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC ATCAGTGAAC CTGCCGCGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA CGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
GTGGAGCAAA ACAAGCTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC 
CCATACAACC AATCATACGA CCAGAACACA GGAGGATCCT ACAACAGCTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA GGGGCACAAG AAAGGGGCAA GAACACCCAA GGTACCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCTCCACAC 
AGCAATAGCG AAGAGTACAA CATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT 
CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAAGATGAAT TAGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CTCCCCCTGT TCGGGGAGCA GCTTCTTCTC CAGCTGCCGT GTCCTATAGC 
CATCAGTCCA CTGCCACTCT GACTCCCTCC CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCAG AGGAGACTGG CCACATGCAG CACCAGCCCG ACAGGAGACG GCAGCCTGTG 
AGTCCTCCTC CACCACCACG GCCGATCTCC CCTCCACATA CCTATGGCTA CATTTCAGGA 
CCCCTGGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAGGTAGCCA AGATGCAAAC CAGAAGGCTT TTGTTACGTG GGCTTGAGCA GACACCTGCC 
TCCAGTGTTG GGGACCTGGA GAGCTCTGTC ACGGGGTCCA TGATCAACGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGTG TTAGTTCTTC GGACGGCTCC 
TTTTTCACTG ATGCTGACTT TGCCCAGGCA GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG 
AAAGTAGCAC GACGGCAAAT GCAGGATGCT GCTGGCCGTC GACATTTTCA TGCGTCTCAG 
TGCCCTAGGC CCACAAGTCC CGTGTCTACA GACAGCAACA TGAGTGCCGC CGTAATGCAG 
AAAACCAGAC CAGCCAAGAA ACTGAAACAC CAGCCAGGAC ATCTGCGCAG AGAAACCTAC 
ACAGATGATC TTCCACCACC TCCTGTGCCG CCACCTGCTA TAAAGTCACC TACTGCCCAA 
TCCAAGACAC AGCTGGAAGT ACGACCTGTA GTGGTGCCAA AACTCCCTTC TATGGATGCA 
AGAACAGACA GATCATCAGA CAGAAAAGGA AGCAGTTACA AGGGGAGAGA AGTGTTGGAT 
GGAAGACAGG TTGTTGACAT GCGAACAAAT CCAGGTGATC CCAGAGAAGC ACAGGAACAG 
CAAAATGACG GGAAAGGACG TGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TATTGTAGAC CTACTTTTCC AACATCAAAT 
AATCCCAGAG ATCCCAGTTC CTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTACT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713340.1
CDS	968..5815
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_006713277.2
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713277.2

ORF Insert Sequence:

ATGATTGCGG AGCCCGCTCA CTTTTACCTG TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCAGA CCTGATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GCCGCCCCAC ACCCACTATT 
GAATGGTACA AAGGGGGAGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CGAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG GAAAAGTAGA 
CCTGATGAAG GAGTCTATGT CTGTGTAGCA AGGAATTACC TTGGAGAGGC TGTGAGCCAC 
AATGCATCGC TGGAAGTAGC CATACTTCGG GATGACTTCA GACAAAACCC TTCGGATGTC 
ATGGTTGCAG TAGGAGAGCC TGCAGTAATG GAATGCCAAC CTCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATCACTTAC ACCCGTAAAA GTGACGCTGG CAAATATGTT 
TGTGTTGGTA CCAATATGGT TGGGGAACGT GAGAGTGAAG TAGCCGAGCT GACTGTCTTA 
GAGAGACCAT CATTTGTGAA GAGACCCAGT AACTTGGCAG TAACTGTGGA TGACAGTGCA 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTACCTACAG TACGATGGAG GAAAGATGAT 
GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACCTTGAA AATTAGGAAG 
GTGACAGCTG GTGACATGGG TTCATACACT TGTGTTGCAG AAAATATGGT GGGCAAAGCT 
GAAGCATCTG CTACTCTGAC TGTTCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGACGG ACTGTAACTT TTCAGTGTGA AGCAACCGGA 
AATCCTCAAC CAGCTATTTT CTGGAGGAGA GAAGGGAGTC AGAATCTACT TTTCTCATAT 
CAACCACCAC AGTCATCCAG CCGATTTTCA GTCTCCCAGA CTGGCGACCT CACAATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAC ATCTGCCAGA CTTTAAATGT TGCTGGAAGC 
ATCATCACAA AGGCATATTT GGAAGTTACA GATGTGATTG CAGATCGGCC TCCCCCAGTT 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCCGTGGATG GCACTTTCGT CCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACCATT CTGTGGAGAA AGGATGGAGT CCTCGTTTCA 
ACCCAAGACT CTCGAATCAA ACAGTTGGAG AATGGAGTAC TGCAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG 
AGTGCTTACA TTGAAGTTCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCTACTGAC 
CCAAATTTAA TCCCTAGTGC CCCATCAAAA CCTGAAGTGA CAGATGTCAG CAGAAATACA 
GTCACATTAT CGTGGCAACC AAATTTGAAT TCAGGAGCAA CTCCAACATC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA 
GAAACATCTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCTAATGCAT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACACAAGAT 
GTCCTACCAA CAAGTCAGGG GGTGGACCAC AAGCAGGTCC AGAGAGAGCT GGGAAATGCT 
GTTCTGCACC TCCACAACCC CACCGTCCTT TCTTCCTCTT CCATCGAAGT GCACTGGACA 
GTAGATCAAC AGTCTCAGTA TATACAAGGA TATAAAATTC TCTATCGGCC ATCTGGAGCC 
AACCACGGAG AATCAGACTG GTTAGTTTTT GAAGTGAGGA CGCCAGCCAA AAACAGTGTG 
GTAATCCCTG ATCTCAGAAA GGGAGTCAAC TATGAAATTA AGGCTCGCCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATCAAG TTTGCCAAAA CCCTGGAAGA AGCACCCAGT 
GCCCCACCCC AAGGTGTAAC TGTATCCAAG AATGATGGAA ACGGAACTGC AATTCTAGTT 
AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTCG ATACCACATC AACAAAACAG TGGATGGTTC CACCTTTTCC 
GTGGTCATTC CCTTTCTTGT TCCTGGAATC CGATACAGTG TGGAAGTGGC AGCCAGCACT 
GGGGCTGGGT CTGGGGTAAA GAGTGAGCCT CAGTTCATCC AGCTGGATGC CCATGGAAAC 
CCTGTGTCAC CTGAGGACCA AGTCAGCCTC GCTCAGCAGA TTTCAGATGT GGTGAAGCAG 
CCGGCCTTCA TAGCAGGTAT TGGAGCAGCC TGTTGGATCA TCCTCATGGT CTTCAGCATC 
TGGCTTTATC GACACCGCAA GAAGAGAAAC GGACTTACTA GTACCTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTACC AGAGAGGAGG CGAAGCTGTC 
AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC ATCAGTGAAC CTGCCGCGCA GCCATGGCTG 
GCAGACACGT GGCCTAATAC TGGCAACAAC CACAATGACT GCTCCATCAG CTGCTGCACG 
GCAGGCAATG GAAACAGCGA CAGCAACCTC ACTACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CTACTCCTTA CGCCACCACT 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCTGGCGAG 
AAGCACTGGA AACCACTGGG ACAGCAGAAA CAAGAAGTGG CACCAGTTCA GTACAACATC 
GTGGAGCAAA ACAAGCTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC 
CCATACAACC AATCATACGA CCAGAACACA GGAGGATCCT ACAACAGCTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA GGGGCACAAG AAAGGGGCAA GAACACCCAA GGTACCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCTCCACAC 
AGCAATAGCG AAGAGTACAA CATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT 
CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAAGATGAAT TAGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CTCCCCCTGT TCGGGGAGCA GCTTCTTCTC CAGCTGCCGT GTCCTATAGC 
CATCAGTCCA CTGCCACTCT GACTCCCTCC CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCAG AGGAGACTGG CCACATGCAG CACCAGCCCG ACAGGAGACG GCAGCCTGTG 
AGTCCTCCTC CACCACCACG GCCGATCTCC CCTCCACATA CCTATGGCTA CATTTCAGGA 
CCCCTGGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAGGTAGCCA AGATGCAAAC CAGAAGGCTT TTGTTACGTG GGCTTGAGCA GACACCTGCC 
TCCAGTGTTG GGGACCTGGA GAGCTCTGTC ACGGGGTCCA TGATCAACGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGTG TTAGTTCTTC GGACGGCTCC 
TTTTTCACTG ATGCTGACTT TGCCCAGGCA GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG 
AAAGTAGCAC GACGGCAAAT GCAGGATGCT GCTGGCCGTC GACATTTTCA TGCGTCTCAG 
TGCCCTAGGC CCACAAGTCC CGTGTCTACA GACAGCAACA TGAGTGCCGC CGTAATGCAG 
AAAACCAGAC CAGCCAAGAA ACTGAAACAC CAGCCAGGAC ATCTGCGCAG AGAAACCTAC 
ACAGATGATC TTCCACCACC TCCTGTGCCG CCACCTGCTA TAAAGTCACC TACTGCCCAA 
TCCAAGACAC AGCTGGAAGT ACGACCTGTA GTGGTGCCAA AACTCCCTTC TATGGATGCA 
AGAACAGACA GATCATCAGA CAGAAAAGGA AGCAGTTACA AGGGGAGAGA AGTGTTGGAT 
GGAAGACAGG TTGTTGACAT GCGAACAAAT CCAGGTGATC CCAGAGAAGC ACAGGAACAG 
CAAAATGACG GGAAAGGACG TGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TATTGTAGAC CTACTTTTCC AACATCAAAT 
AATCCCAGAG ATCCCAGTTC CTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTACT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_011533976.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532278.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

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CloneID	OHu60276
Clone ID Related Accession (Same CDS sequence)	XM_011533976.1 , XM_011533977.2
Accession Version	XM_011533976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCTGGACT TCTCAACATC AGTGAACCTG CCGCGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTACGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCGTG 
GAGCAAAACA AGCTGAACAA AGATTATCGA GCAAATGACA CAGTTCCTCC AACTATCCCA 
TACAACCAAT CATACGACCA GAACACAGGA GGATCCTACA ACAGCTCAGA CCGGGGCAGT 
AGTACATCTG GGAGTCAGGG GCACAAGAAA GGGGCAAGAA CACCCAAGGT ACCAAAACAG 
GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CACATCCTCC TCCACACAGC 
AATAGCGAAG AGTACAACAT TTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGTCCC 
GTGCCACCAG CAAGGATGTA TTTGCAACAA GATGAATTAG AAGAGGAGGA AGATGAACGA 
GGCCCCACTC CCCCTGTTCG GGGAGCAGCT TCTTCTCCAG CTGCCGTGTC CTATAGCCAT 
CAGTCCACTG CCACTCTGAC TCCCTCCCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCAGAGG AGACTGGCCA CATGCAGCAC CAGCCCGACA GGAGACGGCA GCCTGTGAGT 
CCTCCTCCAC CACCACGGCC GATCTCCCCT CCACATACCT ATGGCTACAT TTCAGGACCC 
CTGGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAG 
GTAGCCAAGA TGCAAACCAG AAGGCTTTTG TTACGTGGGC TTGAGCAGAC ACCTGCCTCC 
AGTGTTGGGG ACCTGGAGAG CTCTGTCACG GGGTCCATGA TCAACGGCTG GGGCTCAGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGTGTTA GTTCTTCGGA CGGCTCCTTT 
TTCACTGATG CTGACTTTGC CCAGGCAGTC GCAGCAGCGG CAGAGTATGC TGGTCTGAAA 
GTAGCACGAC GGCAAATGCA GGATGCTGCT GGCCGTCGAC ATTTTCATGC GTCTCAGTGC 
CCTAGGCCCA CAAGTCCCGT GTCTACAGAC AGCAACATGA GTGCCGCCGT AATGCAGAAA 
ACCAGACCAG CCAAGAAACT GAAACACCAG CCAGGACATC TGCGCAGAGA AACCTACACA 
GATGATCTTC CACCACCTCC TGTGCCGCCA CCTGCTATAA AGTCACCTAC TGCCCAATCC 
AAGACACAGC TGGAAGTACG ACCTGTAGTG GTGCCAAAAC TCCCTTCTAT GGATGCAAGA 
ACAGACAGAT CATCAGACAG AAAAGGAAGC AGTTACAAGG GGAGAGAAGT GTTGGATGGA 
AGACAGGTTG TTGACATGCG AACAAATCCA GGTGATCCCA GAGAAGCACA GGAACAGCAA 
AATGACGGGA AAGGACGTGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGACT 
CATCTCATCC AAGAGGATAT TCTACCTTAT TGTAGACCTA CTTTTCCAAC ATCAAATAAT 
CCCAGAGATC CCAGTTCCTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAAATGC AGGTACTTGG AGGATATGAA 
AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532278.1
CDS	298..5262
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_011533976.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533976.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCTGGACT TCTCAACATC AGTGAACCTG CCGCGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTACGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCGTG 
GAGCAAAACA AGCTGAACAA AGATTATCGA GCAAATGACA CAGTTCCTCC AACTATCCCA 
TACAACCAAT CATACGACCA GAACACAGGA GGATCCTACA ACAGCTCAGA CCGGGGCAGT 
AGTACATCTG GGAGTCAGGG GCACAAGAAA GGGGCAAGAA CACCCAAGGT ACCAAAACAG 
GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CACATCCTCC TCCACACAGC 
AATAGCGAAG AGTACAACAT TTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGTCCC 
GTGCCACCAG CAAGGATGTA TTTGCAACAA GATGAATTAG AAGAGGAGGA AGATGAACGA 
GGCCCCACTC CCCCTGTTCG GGGAGCAGCT TCTTCTCCAG CTGCCGTGTC CTATAGCCAT 
CAGTCCACTG CCACTCTGAC TCCCTCCCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCAGAGG AGACTGGCCA CATGCAGCAC CAGCCCGACA GGAGACGGCA GCCTGTGAGT 
CCTCCTCCAC CACCACGGCC GATCTCCCCT CCACATACCT ATGGCTACAT TTCAGGACCC 
CTGGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAG 
GTAGCCAAGA TGCAAACCAG AAGGCTTTTG TTACGTGGGC TTGAGCAGAC ACCTGCCTCC 
AGTGTTGGGG ACCTGGAGAG CTCTGTCACG GGGTCCATGA TCAACGGCTG GGGCTCAGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGTGTTA GTTCTTCGGA CGGCTCCTTT 
TTCACTGATG CTGACTTTGC CCAGGCAGTC GCAGCAGCGG CAGAGTATGC TGGTCTGAAA 
GTAGCACGAC GGCAAATGCA GGATGCTGCT GGCCGTCGAC ATTTTCATGC GTCTCAGTGC 
CCTAGGCCCA CAAGTCCCGT GTCTACAGAC AGCAACATGA GTGCCGCCGT AATGCAGAAA 
ACCAGACCAG CCAAGAAACT GAAACACCAG CCAGGACATC TGCGCAGAGA AACCTACACA 
GATGATCTTC CACCACCTCC TGTGCCGCCA CCTGCTATAA AGTCACCTAC TGCCCAATCC 
AAGACACAGC TGGAAGTACG ACCTGTAGTG GTGCCAAAAC TCCCTTCTAT GGATGCAAGA 
ACAGACAGAT CATCAGACAG AAAAGGAAGC AGTTACAAGG GGAGAGAAGT GTTGGATGGA 
AGACAGGTTG TTGACATGCG AACAAATCCA GGTGATCCCA GAGAAGCACA GGAACAGCAA 
AATGACGGGA AAGGACGTGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGACT 
CATCTCATCC AAGAGGATAT TCTACCTTAT TGTAGACCTA CTTTTCCAAC ATCAAATAAT 
CCCAGAGATC CCAGTTCCTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAAATGC AGGTACTTGG AGGATATGAA 
AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_011533977.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532279.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

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CloneID	OHu60276
Clone ID Related Accession (Same CDS sequence)	XM_011533976.1 , XM_011533977.2
Accession Version	XM_011533977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCTGGACT TCTCAACATC AGTGAACCTG CCGCGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTACGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCGTG 
GAGCAAAACA AGCTGAACAA AGATTATCGA GCAAATGACA CAGTTCCTCC AACTATCCCA 
TACAACCAAT CATACGACCA GAACACAGGA GGATCCTACA ACAGCTCAGA CCGGGGCAGT 
AGTACATCTG GGAGTCAGGG GCACAAGAAA GGGGCAAGAA CACCCAAGGT ACCAAAACAG 
GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CACATCCTCC TCCACACAGC 
AATAGCGAAG AGTACAACAT TTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGTCCC 
GTGCCACCAG CAAGGATGTA TTTGCAACAA GATGAATTAG AAGAGGAGGA AGATGAACGA 
GGCCCCACTC CCCCTGTTCG GGGAGCAGCT TCTTCTCCAG CTGCCGTGTC CTATAGCCAT 
CAGTCCACTG CCACTCTGAC TCCCTCCCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCAGAGG AGACTGGCCA CATGCAGCAC CAGCCCGACA GGAGACGGCA GCCTGTGAGT 
CCTCCTCCAC CACCACGGCC GATCTCCCCT CCACATACCT ATGGCTACAT TTCAGGACCC 
CTGGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAG 
GTAGCCAAGA TGCAAACCAG AAGGCTTTTG TTACGTGGGC TTGAGCAGAC ACCTGCCTCC 
AGTGTTGGGG ACCTGGAGAG CTCTGTCACG GGGTCCATGA TCAACGGCTG GGGCTCAGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGTGTTA GTTCTTCGGA CGGCTCCTTT 
TTCACTGATG CTGACTTTGC CCAGGCAGTC GCAGCAGCGG CAGAGTATGC TGGTCTGAAA 
GTAGCACGAC GGCAAATGCA GGATGCTGCT GGCCGTCGAC ATTTTCATGC GTCTCAGTGC 
CCTAGGCCCA CAAGTCCCGT GTCTACAGAC AGCAACATGA GTGCCGCCGT AATGCAGAAA 
ACCAGACCAG CCAAGAAACT GAAACACCAG CCAGGACATC TGCGCAGAGA AACCTACACA 
GATGATCTTC CACCACCTCC TGTGCCGCCA CCTGCTATAA AGTCACCTAC TGCCCAATCC 
AAGACACAGC TGGAAGTACG ACCTGTAGTG GTGCCAAAAC TCCCTTCTAT GGATGCAAGA 
ACAGACAGAT CATCAGACAG AAAAGGAAGC AGTTACAAGG GGAGAGAAGT GTTGGATGGA 
AGACAGGTTG TTGACATGCG AACAAATCCA GGTGATCCCA GAGAAGCACA GGAACAGCAA 
AATGACGGGA AAGGACGTGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGACT 
CATCTCATCC AAGAGGATAT TCTACCTTAT TGTAGACCTA CTTTTCCAAC ATCAAATAAT 
CCCAGAGATC CCAGTTCCTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAAATGC AGGTACTTGG AGGATATGAA 
AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532279.1
CDS	151..5115
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_011533977.2
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533977.2

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCTGGACT TCTCAACATC AGTGAACCTG CCGCGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCTA CTCCTTACGC CACCACTCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC TGGCGAGAAG 
CACTGGAAAC CACTGGGACA GCAGAAACAA GAAGTGGCAC CAGTTCAGTA CAACATCGTG 
GAGCAAAACA AGCTGAACAA AGATTATCGA GCAAATGACA CAGTTCCTCC AACTATCCCA 
TACAACCAAT CATACGACCA GAACACAGGA GGATCCTACA ACAGCTCAGA CCGGGGCAGT 
AGTACATCTG GGAGTCAGGG GCACAAGAAA GGGGCAAGAA CACCCAAGGT ACCAAAACAG 
GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CACATCCTCC TCCACACAGC 
AATAGCGAAG AGTACAACAT TTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGTCCC 
GTGCCACCAG CAAGGATGTA TTTGCAACAA GATGAATTAG AAGAGGAGGA AGATGAACGA 
GGCCCCACTC CCCCTGTTCG GGGAGCAGCT TCTTCTCCAG CTGCCGTGTC CTATAGCCAT 
CAGTCCACTG CCACTCTGAC TCCCTCCCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCAGAGG AGACTGGCCA CATGCAGCAC CAGCCCGACA GGAGACGGCA GCCTGTGAGT 
CCTCCTCCAC CACCACGGCC GATCTCCCCT CCACATACCT ATGGCTACAT TTCAGGACCC 
CTGGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAG 
GTAGCCAAGA TGCAAACCAG AAGGCTTTTG TTACGTGGGC TTGAGCAGAC ACCTGCCTCC 
AGTGTTGGGG ACCTGGAGAG CTCTGTCACG GGGTCCATGA TCAACGGCTG GGGCTCAGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGTGTTA GTTCTTCGGA CGGCTCCTTT 
TTCACTGATG CTGACTTTGC CCAGGCAGTC GCAGCAGCGG CAGAGTATGC TGGTCTGAAA 
GTAGCACGAC GGCAAATGCA GGATGCTGCT GGCCGTCGAC ATTTTCATGC GTCTCAGTGC 
CCTAGGCCCA CAAGTCCCGT GTCTACAGAC AGCAACATGA GTGCCGCCGT AATGCAGAAA 
ACCAGACCAG CCAAGAAACT GAAACACCAG CCAGGACATC TGCGCAGAGA AACCTACACA 
GATGATCTTC CACCACCTCC TGTGCCGCCA CCTGCTATAA AGTCACCTAC TGCCCAATCC 
AAGACACAGC TGGAAGTACG ACCTGTAGTG GTGCCAAAAC TCCCTTCTAT GGATGCAAGA 
ACAGACAGAT CATCAGACAG AAAAGGAAGC AGTTACAAGG GGAGAGAAGT GTTGGATGGA 
AGACAGGTTG TTGACATGCG AACAAATCCA GGTGATCCCA GAGAAGCACA GGAACAGCAA 
AATGACGGGA AAGGACGTGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGACT 
CATCTCATCC AAGAGGATAT TCTACCTTAT TGTAGACCTA CTTTTCCAAC ATCAAATAAT 
CCCAGAGATC CCAGTTCCTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAAATGC AGGTACTTGG AGGATATGAA 
AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_011533978.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532280.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu60277
Clone ID Related Accession (Same CDS sequence)	XM_011533978.1
Accession Version	XM_011533978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC 
ATCAGTGAAC CTGCCGCGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CTACTCCTTA CGCCACCACT CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGCGGGGA CTCTGGCGAG AAGCACTGGA AACCACTGGG ACAGCAGAAA 
CAAGAAGTGG CACCAGTTCA GTACAACATC GTGGAGCAAA ACAAGCTGAA CAAAGATTAT 
CGAGCAAATG ACACAGTTCC TCCAACTATC CCATACAACC AATCATACGA CCAGAACACA 
GGAGGATCCT ACAACAGCTC AGACCGGGGC AGTAGTACAT CTGGGAGTCA GGGGCACAAG 
AAAGGGGCAA GAACACCCAA GGTACCAAAA CAGGGTGGCA TGAACTGGGC AGACCTGCTT 
CCTCCTCCCC CAGCACATCC TCCTCCACAC AGCAATAGCG AAGAGTACAA CATTTCTGTA 
GATGAAAGCT ATGACCAAGA AATGCCATGT CCCGTGCCAC CAGCAAGGAT GTATTTGCAA 
CAAGATGAAT TAGAAGAGGA GGAAGATGAA CGAGGCCCCA CTCCCCCTGT TCGGGGAGCA 
GCTTCTTCTC CAGCTGCCGT GTCCTATAGC CATCAGTCCA CTGCCACTCT GACTCCCTCC 
CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCAG AGGAGACTGG CCACATGCAG 
CACCAGCCCG ACAGGAGACG GCAGCCTGTG AGTCCTCCTC CACCACCACG GCCGATCTCC 
CCTCCACATA CCTATGGCTA CATTTCAGGA CCCCTGGTCT CAGATATGGA TACGGATGCG 
CCAGAAGAGG AAGAAGACGA AGCCGACATG GAGGTAGCCA AGATGCAAAC CAGAAGGCTT 
TTGTTACGTG GGCTTGAGCA GACACCTGCC TCCAGTGTTG GGGACCTGGA GAGCTCTGTC 
ACGGGGTCCA TGATCAACGG CTGGGGCTCA GCCTCAGAGG AGGACAACAT TTCCAGCGGA 
CGCTCCAGTG TTAGTTCTTC GGACGGCTCC TTTTTCACTG ATGCTGACTT TGCCCAGGCA 
GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG AAAGTAGCAC GACGGCAAAT GCAGGATGCT 
GCTGGCCGTC GACATTTTCA TGCGTCTCAG TGCCCTAGGC CCACAAGTCC CGTGTCTACA 
GACAGCAACA TGAGTGCCGC CGTAATGCAG AAAACCAGAC CAGCCAAGAA ACTGAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAACCTAC ACAGATGATC TTCCACCACC TCCTGTGCCG 
CCACCTGCTA TAAAGTCACC TACTGCCCAA TCCAAGACAC AGCTGGAAGT ACGACCTGTA 
GTGGTGCCAA AACTCCCTTC TATGGATGCA AGAACAGACA GATCATCAGA CAGAAAAGGA 
AGCAGTTACA AGGGGAGAGA AGTGTTGGAT GGAAGACAGG TTGTTGACAT GCGAACAAAT 
CCAGGTGATC CCAGAGAAGC ACAGGAACAG CAAAATGACG GGAAAGGACG TGGAAACAAG 
GCAGCAAAAC GAGACCTTCC ACCAGCAAAG ACTCATCTCA TCCAAGAGGA TATTCTACCT 
TATTGTAGAC CTACTTTTCC AACATCAAAT AATCCCAGAG ATCCCAGTTC CTCAAGCTCA 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAAA TGCAGGTACT TGGAGGATAT GAAAGAGGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532280.1
CDS	298..5235
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_011533978.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533978.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC 
ATCAGTGAAC CTGCCGCGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CTACTCCTTA CGCCACCACT CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGCGGGGA CTCTGGCGAG AAGCACTGGA AACCACTGGG ACAGCAGAAA 
CAAGAAGTGG CACCAGTTCA GTACAACATC GTGGAGCAAA ACAAGCTGAA CAAAGATTAT 
CGAGCAAATG ACACAGTTCC TCCAACTATC CCATACAACC AATCATACGA CCAGAACACA 
GGAGGATCCT ACAACAGCTC AGACCGGGGC AGTAGTACAT CTGGGAGTCA GGGGCACAAG 
AAAGGGGCAA GAACACCCAA GGTACCAAAA CAGGGTGGCA TGAACTGGGC AGACCTGCTT 
CCTCCTCCCC CAGCACATCC TCCTCCACAC AGCAATAGCG AAGAGTACAA CATTTCTGTA 
GATGAAAGCT ATGACCAAGA AATGCCATGT CCCGTGCCAC CAGCAAGGAT GTATTTGCAA 
CAAGATGAAT TAGAAGAGGA GGAAGATGAA CGAGGCCCCA CTCCCCCTGT TCGGGGAGCA 
GCTTCTTCTC CAGCTGCCGT GTCCTATAGC CATCAGTCCA CTGCCACTCT GACTCCCTCC 
CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCAG AGGAGACTGG CCACATGCAG 
CACCAGCCCG ACAGGAGACG GCAGCCTGTG AGTCCTCCTC CACCACCACG GCCGATCTCC 
CCTCCACATA CCTATGGCTA CATTTCAGGA CCCCTGGTCT CAGATATGGA TACGGATGCG 
CCAGAAGAGG AAGAAGACGA AGCCGACATG GAGGTAGCCA AGATGCAAAC CAGAAGGCTT 
TTGTTACGTG GGCTTGAGCA GACACCTGCC TCCAGTGTTG GGGACCTGGA GAGCTCTGTC 
ACGGGGTCCA TGATCAACGG CTGGGGCTCA GCCTCAGAGG AGGACAACAT TTCCAGCGGA 
CGCTCCAGTG TTAGTTCTTC GGACGGCTCC TTTTTCACTG ATGCTGACTT TGCCCAGGCA 
GTCGCAGCAG CGGCAGAGTA TGCTGGTCTG AAAGTAGCAC GACGGCAAAT GCAGGATGCT 
GCTGGCCGTC GACATTTTCA TGCGTCTCAG TGCCCTAGGC CCACAAGTCC CGTGTCTACA 
GACAGCAACA TGAGTGCCGC CGTAATGCAG AAAACCAGAC CAGCCAAGAA ACTGAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAACCTAC ACAGATGATC TTCCACCACC TCCTGTGCCG 
CCACCTGCTA TAAAGTCACC TACTGCCCAA TCCAAGACAC AGCTGGAAGT ACGACCTGTA 
GTGGTGCCAA AACTCCCTTC TATGGATGCA AGAACAGACA GATCATCAGA CAGAAAAGGA 
AGCAGTTACA AGGGGAGAGA AGTGTTGGAT GGAAGACAGG TTGTTGACAT GCGAACAAAT 
CCAGGTGATC CCAGAGAAGC ACAGGAACAG CAAAATGACG GGAAAGGACG TGGAAACAAG 
GCAGCAAAAC GAGACCTTCC ACCAGCAAAG ACTCATCTCA TCCAAGAGGA TATTCTACCT 
TATTGTAGAC CTACTTTTCC AACATCAAAT AATCCCAGAG ATCCCAGTTC CTCAAGCTCA 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAAA TGCAGGTACT TGGAGGATAT GAAAGAGGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_011533979.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532281.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

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CloneID	OHu60278
Clone ID Related Accession (Same CDS sequence)	XM_011533979.1
Accession Version	XM_011533979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCTGGACT TCTCAACATC AGTGAACCTG CCGCGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGGGCA GCCTACTCCT 
TACGCCACCA CTCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGCGGG 
GACTCTGGCG AGAAGCACTG GAAACCACTG GGACAGCAGA AACAAGAAGT GGCACCAGTT 
CAGTACAACA TCGTGGAGCA AAACAAGCTG AACAAAGATT ATCGAGCAAA TGACACAGTT 
CCTCCAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCT GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGACGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTAGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCCGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACTGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTATGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTGTTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532281.1
CDS	298..5097
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_011533979.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533979.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTACCAGA GAGGAGGCGA AGCTGTCAGC 
AGTGGAGGGA GGCCTGGACT TCTCAACATC AGTGAACCTG CCGCGCAGCC ATGGCTGGCA 
GACACGTGGC CTAATACTGG CAACAACCAC AATGACTGCT CCATCAGCTG CTGCACGGCA 
GGCAATGGAA ACAGCGACAG CAACCTCACT ACCTACAGTC GCCCAGGGCA GCCTACTCCT 
TACGCCACCA CTCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGCGGG 
GACTCTGGCG AGAAGCACTG GAAACCACTG GGACAGCAGA AACAAGAAGT GGCACCAGTT 
CAGTACAACA TCGTGGAGCA AAACAAGCTG AACAAAGATT ATCGAGCAAA TGACACAGTT 
CCTCCAACTA TCCCATACAA CCAATCATAC GACCAGAACA CAGGAGGATC CTACAACAGC 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAGGGGCACA AGAAAGGGGC AAGAACACCC 
AAGGTACCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCTCCAC ACAGCAATAG CGAAGAGTAC AACATTTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAAGATGA ATTAGAAGAG 
GAGGAAGATG AACGAGGCCC CACTCCCCCT GTTCGGGGAG CAGCTTCTTC TCCAGCTGCC 
GTGTCCTATA GCCATCAGTC CACTGCCACT CTGACTCCCT CCCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC AGAGGAGACT GGCCACATGC AGCACCAGCC CGACAGGAGA 
CGGCAGCCTG TGAGTCCTCC TCCACCACCA CGGCCGATCT CCCCTCCACA TACCTATGGC 
TACATTTCAG GACCCCTGGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAGGTAGC CAAGATGCAA ACCAGAAGGC TTTTGTTACG TGGGCTTGAG 
CAGACACCTG CCTCCAGTGT TGGGGACCTG GAGAGCTCTG TCACGGGGTC CATGATCAAC 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG TGTTAGTTCT 
TCGGACGGCT CCTTTTTCAC TGATGCTGAC TTTGCCCAGG CAGTCGCAGC AGCGGCAGAG 
TATGCTGGTC TGAAAGTAGC ACGACGGCAA ATGCAGGATG CTGCTGGCCG TCGACATTTT 
CATGCGTCTC AGTGCCCTAG GCCCACAAGT CCCGTGTCTA CAGACAGCAA CATGAGTGCC 
GCCGTAATGC AGAAAACCAG ACCAGCCAAG AAACTGAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAACCT ACACAGATGA TCTTCCACCA CCTCCTGTGC CGCCACCTGC TATAAAGTCA 
CCTACTGCCC AATCCAAGAC ACAGCTGGAA GTACGACCTG TAGTGGTGCC AAAACTCCCT 
TCTATGGATG CAAGAACAGA CAGATCATCA GACAGAAAAG GAAGCAGTTA CAAGGGGAGA 
GAAGTGTTGG ATGGAAGACA GGTTGTTGAC ATGCGAACAA ATCCAGGTGA TCCCAGAGAA 
GCACAGGAAC AGCAAAATGA CGGGAAAGGA CGTGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTATTGTAG ACCTACTTTT 
CCAACATCAA ATAATCCCAG AGATCCCAGT TCCTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTA 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_011533980.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532282.1 Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

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CloneID	OHu60279
Clone ID Related Accession (Same CDS sequence)	XM_011533980.1
Accession Version	XM_011533980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC 
ATCAGTGAAC CTGCCGCGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGG GCAGCCTACT CCTTACGCCA CCACTCAGCT CATCCAGTCA 
AACCTCAGCA ACAACATGAA CAATGGCAGC GGGGACTCTG GCGAGAAGCA CTGGAAACCA 
CTGGGACAGC AGAAACAAGA AGTGGCACCA GTTCAGTACA ACATCGTGGA GCAAAACAAG 
CTGAACAAAG ATTATCGAGC AAATGACACA GTTCCTCCAA CTATCCCATA CAACCAATCA 
TACGACCAGA ACACAGGAGG ATCCTACAAC AGCTCAGACC GGGGCAGTAG TACATCTGGG 
AGTCAGGGGC ACAAGAAAGG GGCAAGAACA CCCAAGGTAC CAAAACAGGG TGGCATGAAC 
TGGGCAGACC TGCTTCCTCC TCCCCCAGCA CATCCTCCTC CACACAGCAA TAGCGAAGAG 
TACAACATTT CTGTAGATGA AAGCTATGAC CAAGAAATGC CATGTCCCGT GCCACCAGCA 
AGGATGTATT TGCAACAAGA TGAATTAGAA GAGGAGGAAG ATGAACGAGG CCCCACTCCC 
CCTGTTCGGG GAGCAGCTTC TTCTCCAGCT GCCGTGTCCT ATAGCCATCA GTCCACTGCC 
ACTCTGACTC CCTCCCCACA GGAAGAACTC CAGCCCATGT TACAGGATTG TCCAGAGGAG 
ACTGGCCACA TGCAGCACCA GCCCGACAGG AGACGGCAGC CTGTGAGTCC TCCTCCACCA 
CCACGGCCGA TCTCCCCTCC ACATACCTAT GGCTACATTT CAGGACCCCT GGTCTCAGAT 
ATGGATACGG ATGCGCCAGA AGAGGAAGAA GACGAAGCCG ACATGGAGGT AGCCAAGATG 
CAAACCAGAA GGCTTTTGTT ACGTGGGCTT GAGCAGACAC CTGCCTCCAG TGTTGGGGAC 
CTGGAGAGCT CTGTCACGGG GTCCATGATC AACGGCTGGG GCTCAGCCTC AGAGGAGGAC 
AACATTTCCA GCGGACGCTC CAGTGTTAGT TCTTCGGACG GCTCCTTTTT CACTGATGCT 
GACTTTGCCC AGGCAGTCGC AGCAGCGGCA GAGTATGCTG GTCTGAAAGT AGCACGACGG 
CAAATGCAGG ATGCTGCTGG CCGTCGACAT TTTCATGCGT CTCAGTGCCC TAGGCCCACA 
AGTCCCGTGT CTACAGACAG CAACATGAGT GCCGCCGTAA TGCAGAAAAC CAGACCAGCC 
AAGAAACTGA AACACCAGCC AGGACATCTG CGCAGAGAAA CCTACACAGA TGATCTTCCA 
CCACCTCCTG TGCCGCCACC TGCTATAAAG TCACCTACTG CCCAATCCAA GACACAGCTG 
GAAGTACGAC CTGTAGTGGT GCCAAAACTC CCTTCTATGG ATGCAAGAAC AGACAGATCA 
TCAGACAGAA AAGGAAGCAG TTACAAGGGG AGAGAAGTGT TGGATGGAAG ACAGGTTGTT 
GACATGCGAA CAAATCCAGG TGATCCCAGA GAAGCACAGG AACAGCAAAA TGACGGGAAA 
GGACGTGGAA ACAAGGCAGC AAAACGAGAC CTTCCACCAG CAAAGACTCA TCTCATCCAA 
GAGGATATTC TACCTTATTG TAGACCTACT TTTCCAACAT CAAATAATCC CAGAGATCCC 
AGTTCCTCAA GCTCAATGTC ATCAAGAGGA TCAGGAAGCA GACAAAGAGA ACAAGCAAAT 
GTAGGTCGAA GAAATATTGC AGAAATGCAG GTACTTGGAG GATATGAAAG AGGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532282.1
CDS	298..5070
Translation	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_011533980.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533980.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATGATATCAC TCCTCAGCTT ATCCCCAAAT 
CACCTGTTTC TGGCCCAGCT TATTCCAGAC CCTGAAGATG TAGAGAGGGG GAACGACCAC 
GGGACGCCAA TCCCCACCTC TGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCAGACCT GATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGCC GCCCCACACC CACTATTGAA 
TGGTACAAAG GGGGAGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCGA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGGAA AAGTAGACCT 
GATGAAGGAG TCTATGTCTG TGTAGCAAGG AATTACCTTG GAGAGGCTGT GAGCCACAAT 
GCATCGCTGG AAGTAGCCAT ACTTCGGGAT GACTTCAGAC AAAACCCTTC GGATGTCATG 
GTTGCAGTAG GAGAGCCTGC AGTAATGGAA TGCCAACCTC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGTAAAAGTG ACGCTGGCAA ATATGTTTGT 
GTTGGTACCA ATATGGTTGG GGAACGTGAG AGTGAAGTAG CCGAGCTGAC TGTCTTAGAG 
AGACCATCAT TTGTGAAGAG ACCCAGTAAC TTGGCAGTAA CTGTGGATGA CAGTGCAGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTA CCTACAGTAC GATGGAGGAA AGATGATGGA 
GAGCTGCCCA AATCCAGATA TGAAATCCGA GATGATCATA CCTTGAAAAT TAGGAAGGTG 
ACAGCTGGTG ACATGGGTTC ATACACTTGT GTTGCAGAAA ATATGGTGGG CAAAGCTGAA 
GCATCTGCTA CTCTGACTGT TCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGACGGACT GTAACTTTTC AGTGTGAAGC AACCGGAAAT 
CCTCAACCAG CTATTTTCTG GAGGAGAGAA GGGAGTCAGA ATCTACTTTT CTCATATCAA 
CCACCACAGT CATCCAGCCG ATTTTCAGTC TCCCAGACTG GCGACCTCAC AATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTACATC TGCCAGACTT TAAATGTTGC TGGAAGCATC 
ATCACAAAGG CATATTTGGA AGTTACAGAT GTGATTGCAG ATCGGCCTCC CCCAGTTATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCC GTGGATGGCA CTTTCGTCCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACCATTCTG TGGAGAAAGG ATGGAGTCCT CGTTTCAACC 
CAAGACTCTC GAATCAAACA GTTGGAGAAT GGAGTACTGC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT 
GCTTACATTG AAGTTCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC TACTGACCCA 
AATTTAATCC CTAGTGCCCC ATCAAAACCT GAAGTGACAG ATGTCAGCAG AAATACAGTC 
ACATTATCGT GGCAACCAAA TTTGAATTCA GGAGCAACTC CAACATCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA 
ACATCTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCT 
AATGCATATG GAATTAGTGA TCCAAGCCAA ATATCAGATC CAGTGAAAAC ACAAGATGTC 
CTACCAACAA GTCAGGGGGT GGACCACAAG CAGGTCCAGA GAGAGCTGGG AAATGCTGTT 
CTGCACCTCC ACAACCCCAC CGTCCTTTCT TCCTCTTCCA TCGAAGTGCA CTGGACAGTA 
GATCAACAGT CTCAGTATAT ACAAGGATAT AAAATTCTCT ATCGGCCATC TGGAGCCAAC 
CACGGAGAAT CAGACTGGTT AGTTTTTGAA GTGAGGACGC CAGCCAAAAA CAGTGTGGTA 
ATCCCTGATC TCAGAAAGGG AGTCAACTAT GAAATTAAGG CTCGCCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATCAAGTTT GCCAAAACCC TGGAAGAAGC ACCCAGTGCC 
CCACCCCAAG GTGTAACTGT ATCCAAGAAT GATGGAAACG GAACTGCAAT TCTAGTTAGT 
TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTCGATA CCACATCAAC AAAACAGTGG ATGGTTCCAC CTTTTCCGTG 
GTCATTCCCT TTCTTGTTCC TGGAATCCGA TACAGTGTGG AAGTGGCAGC CAGCACTGGG 
GCTGGGTCTG GGGTAAAGAG TGAGCCTCAG TTCATCCAGC TGGATGCCCA TGGAAACCCT 
GTGTCACCTG AGGACCAAGT CAGCCTCGCT CAGCAGATTT CAGATGTGGT GAAGCAGCCG 
GCCTTCATAG CAGGTATTGG AGCAGCCTGT TGGATCATCC TCATGGTCTT CAGCATCTGG 
CTTTATCGAC ACCGCAAGAA GAGAAACGGA CTTACTAGTA CCTACGCGGG TATCAGAAAA 
GTAACTTACC AGAGAGGAGG CGAAGCTGTC AGCAGTGGAG GGAGGCCTGG ACTTCTCAAC 
ATCAGTGAAC CTGCCGCGCA GCCATGGCTG GCAGACACGT GGCCTAATAC TGGCAACAAC 
CACAATGACT GCTCCATCAG CTGCTGCACG GCAGGCAATG GAAACAGCGA CAGCAACCTC 
ACTACCTACA GTCGCCCAGG GCAGCCTACT CCTTACGCCA CCACTCAGCT CATCCAGTCA 
AACCTCAGCA ACAACATGAA CAATGGCAGC GGGGACTCTG GCGAGAAGCA CTGGAAACCA 
CTGGGACAGC AGAAACAAGA AGTGGCACCA GTTCAGTACA ACATCGTGGA GCAAAACAAG 
CTGAACAAAG ATTATCGAGC AAATGACACA GTTCCTCCAA CTATCCCATA CAACCAATCA 
TACGACCAGA ACACAGGAGG ATCCTACAAC AGCTCAGACC GGGGCAGTAG TACATCTGGG 
AGTCAGGGGC ACAAGAAAGG GGCAAGAACA CCCAAGGTAC CAAAACAGGG TGGCATGAAC 
TGGGCAGACC TGCTTCCTCC TCCCCCAGCA CATCCTCCTC CACACAGCAA TAGCGAAGAG 
TACAACATTT CTGTAGATGA AAGCTATGAC CAAGAAATGC CATGTCCCGT GCCACCAGCA 
AGGATGTATT TGCAACAAGA TGAATTAGAA GAGGAGGAAG ATGAACGAGG CCCCACTCCC 
CCTGTTCGGG GAGCAGCTTC TTCTCCAGCT GCCGTGTCCT ATAGCCATCA GTCCACTGCC 
ACTCTGACTC CCTCCCCACA GGAAGAACTC CAGCCCATGT TACAGGATTG TCCAGAGGAG 
ACTGGCCACA TGCAGCACCA GCCCGACAGG AGACGGCAGC CTGTGAGTCC TCCTCCACCA 
CCACGGCCGA TCTCCCCTCC ACATACCTAT GGCTACATTT CAGGACCCCT GGTCTCAGAT 
ATGGATACGG ATGCGCCAGA AGAGGAAGAA GACGAAGCCG ACATGGAGGT AGCCAAGATG 
CAAACCAGAA GGCTTTTGTT ACGTGGGCTT GAGCAGACAC CTGCCTCCAG TGTTGGGGAC 
CTGGAGAGCT CTGTCACGGG GTCCATGATC AACGGCTGGG GCTCAGCCTC AGAGGAGGAC 
AACATTTCCA GCGGACGCTC CAGTGTTAGT TCTTCGGACG GCTCCTTTTT CACTGATGCT 
GACTTTGCCC AGGCAGTCGC AGCAGCGGCA GAGTATGCTG GTCTGAAAGT AGCACGACGG 
CAAATGCAGG ATGCTGCTGG CCGTCGACAT TTTCATGCGT CTCAGTGCCC TAGGCCCACA 
AGTCCCGTGT CTACAGACAG CAACATGAGT GCCGCCGTAA TGCAGAAAAC CAGACCAGCC 
AAGAAACTGA AACACCAGCC AGGACATCTG CGCAGAGAAA CCTACACAGA TGATCTTCCA 
CCACCTCCTG TGCCGCCACC TGCTATAAAG TCACCTACTG CCCAATCCAA GACACAGCTG 
GAAGTACGAC CTGTAGTGGT GCCAAAACTC CCTTCTATGG ATGCAAGAAC AGACAGATCA 
TCAGACAGAA AAGGAAGCAG TTACAAGGGG AGAGAAGTGT TGGATGGAAG ACAGGTTGTT 
GACATGCGAA CAAATCCAGG TGATCCCAGA GAAGCACAGG AACAGCAAAA TGACGGGAAA 
GGACGTGGAA ACAAGGCAGC AAAACGAGAC CTTCCACCAG CAAAGACTCA TCTCATCCAA 
GAGGATATTC TACCTTATTG TAGACCTACT TTTCCAACAT CAAATAATCC CAGAGATCCC 
AGTTCCTCAA GCTCAATGTC ATCAAGAGGA TCAGGAAGCA GACAAAGAGA ACAAGCAAAT 
GTAGGTCGAA GAAATATTGC AGAAATGCAG GTACTTGGAG GATATGAAAG AGGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ROBO1
Entrez Gene ID	6091
Full Name	roundabout guidance receptor 1
Synonyms	DUTT1,SAX3
General protein information	Preferred Names roundabout guidance receptor 1 Names roundabout homolog 1 deleted in U twenty twenty roundabout, axon guidance receptor, homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p12.3
Summary	Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009].
Disorder MIM:	602430

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010242	XP_554155.3
Danio rerio (zebrafish)	robo1	NP_571556.1
Drosophila melanogaster (fruit fly)	robo3	NP_001259866.1
Homo sapiens (human)	ROBO1	NP_002932.1
Pan troglodytes (chimpanzee)	ROBO1	XP_516592.3
Gallus gallus (chicken)	ROBO1	XP_004938403.1
Caenorhabditis elegans (roundworm)	sax-3	NP_001024990.1
Xenopus tropicalis (tropical clawed frog)	robo1	NP_001096171.1
	ROBO1	XP_002802665.1
Canis lupus familiaris (dog)	ROBO1	XP_544814.4
Bos taurus (cattle)	ROBO1	NP_001179817.2
Mus musculus (house mouse)	Robo1	NP_062286.2
Rattus norvegicus (Norway rat)	Robo1	NP_071524.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ROBO1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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