robo1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012956258.2	XP_012811712.2	roundabout homolog 1 isoform X2
XM_012956257.2	XP_012811711.1	roundabout homolog 1 isoform X7
XM_012956263.2	XP_012811717.1	roundabout homolog 1 isoform X8
XM_012956256.2	XP_012811710.2	roundabout homolog 1 isoform X1
XM_012956261.3	XP_012811715.3	roundabout homolog 1 isoform X5
XM_012956262.3	XP_012811716.3	roundabout homolog 1 isoform X6
XM_012956264.2	XP_012811718.1	roundabout homolog 1 isoform X9
XM_012956257.3	XP_012811711.2	roundabout homolog 1 isoform X7
XM_012956259.2	XP_012811713.2	roundabout homolog 1 isoform X3
XM_012956261.2	XP_012811715.2	roundabout homolog 1 isoform X5
XM_012956262.2	XP_012811716.2	roundabout homolog 1 isoform X6
XM_012956263.3	XP_012811717.2	roundabout homolog 1 isoform X8
XM_012956260.2	XP_012811714.2	roundabout homolog 1 isoform X4
XM_012956256.3	XP_012811710.3	roundabout homolog 1 isoform X1
XM_012956258.3	XP_012811712.3	roundabout homolog 1 isoform X2
XM_012956259.3	XP_012811713.3	roundabout homolog 1 isoform X3
XM_012956260.3	XP_012811714.3	roundabout homolog 1 isoform X4
NM_001102701.1	NP_001096171.1	roundabout homolog 1 precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42423	XM_012956256.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa42424	XM_012956258.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa42425	XM_012956259.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa42426	XM_012956260.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa42427	XM_012956261.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa42428	XM_012956262.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa51062	XM_012956256.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51063	XM_012956258.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51064	XM_012956259.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51065	XM_012956260.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51066	XM_012956261.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51067	XM_012956262.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa51068	XM_012956257.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa51069	XM_012956263.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa23658	NM_001102701.1 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa30328	XM_012956257.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa30334	XM_012956263.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa30335	XM_012956264.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

robo1 ( XM_012956256.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811710.2 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa42423
Clone ID Related Accession (Same CDS sequence)	XM_012956256.2
Accession Version	XM_012956256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTGGCG TACCAGCGAG GAGGGGAAGC TGTAAGCAGC 
GCAGGCCGGC CTGGGCTCTT AAATATTTCT GAGCCTGCTA ATCAGCCGTG GCTCGCAGAT 
ACCTGGCCAA ACACTGGAAA CAATCACAAC GAATGCCCCA TCAACTGCTG CACCTCCGGG 
AACGGCAACA GCGACAGCAA CCTCGCCACT TACAGCCGCC CAGCTGACTG CATAGCGAAT 
TACAACAACC AGTTGGACAG CAAGCAGACA AACCTGATGG TGCCCGACTC GGCTGTTTAT 
GGGGATGTAG ACTTAAGCAA CAAGATCAAT GAGATGAAAA CCTTCAACAG CCCCAATCTG 
AAAGATGGGA GGTTTGTCAA TCCTGCCGGC CAGCCCACCC CTTATGCCAC CACTCAGCTC 
ATCCAGTCCA ACATAAGCAA CAACATCAAC ACGTCTGCCG GTGATGTGGA TGAAAAACAC 
TGGAAACCCC CGTGCCAACA GAAACCGGAT GTTACCCCAA TGCAGTACAA CATCATGGAG 
CAAAACAAGA TCAACAAAGA TTACCGAGCT TCGGACACCA TTCCTTCCAC CATTCCCTAC 
AACCAGTCCC ATGATCAGAA TGCCGGAGGG TCATACAACA GCTCAGACCG GGGAAGTAGC 
ACATCTGGGA GCCAAGGTCA TAAGAAGAGT ACTCGTACCC CGAAAGTATC AAAGCAGGCT 
GGCATGAACT GGGCAGATCT TTTGCCACCA CCACCTGCTC ACCCCCCACC ACATGGTGCC 
ACTGAAGACT ACACTTTGTC AGCAGATGAA AGCTATGACA GAGAGTTACC TTGCCCAATA 
CCACCTACAA GAATGTATCT TCAGCAAGAC GAACTGGAAG AGGAAGATGA AAGGGGTCCC 
ACACCTCCTA TTAGAGGAGC AGCTTCTTCT CCGGCTGCTG TGTCCTACAG CCATCAGTCC 
ACTGCCACTC TTACCCCATC ACCTCAGGAC GAACTCCAGC CAATGCTTCA GGACTGTCCC 
GAGGAGATCG GTCATACTCA GCATCAGCTA GAAAGAAGGC GTCATCAAAT TAGTCCTCCT 
CCACCTCCAA GGCCCATTTC CCCGCCTCAT ACATATGGTT ATATTTCTGG ACCAATAGTC 
TCCGATATGG ATACTGATGT TCCTGAAGAG GAAGGGGATG ATGGGGAAAT TGAAGTCACC 
AAGATGCAAA GTCGCAGACT TCTTTTACGT GGCCTGGAGC AGACTCCTGC TTCCAGTGTT 
GGAGACCTAG AAAGTTCTGT GACTGGTTCC ATGATCAATG GATGGGGCTC AGCTTCTGAA 
GAAGACAATG TTTCCAGTGG ACGTTCCAGT GTCAGTTCAT CGGATGGATC ATTTTTCACG 
GATGCGGATT TTGCACAGGC TGTCGCCGCA GCCGCAGAAT ATGCTGGGCT TAAAGTAGCA 
AGGCATCAAA TGCAGGAAGC AGCAGGAGTG TCGCGTCTTT GCCTCCCAGG TCGAAGACCT 
TTCCACGCGT CTCACTGTCC AAGACCAACA AGCCCAGTGT CAACAGACAG CAACATGAGC 
ACCGCTGTCA TACAGAAAAG CCGTCCTGCC AAAAAACAAA AACACCAGCA GGGACATCTT 
CGGAAAGATG TCTACACAGA TGATTTGCCA CCACCGCCAC TGCCACCTCC TGCTATCAAA 
TCTCCAACTG GTCCATCCAA AGCACAACTA GAAGGACGGC CTGGCCTTTC ACAGAAGTAC 
CTTGTTGCAG AACAAAGACT GGAAAGGCCA GGAGAGAGGA AAGGTGTCAG CTACAGGGGC 
AGGGAAGGTC CTGACAGCAG ACAACAACAT GCAGAAGCAA GAACCAATTC AGGGGAGAGA 
AGAGAGTCTT CGGAACAGCA ACTTGATGGA AAGATGAGAA AAGGGAAAGC TTCAAAGTGG 
GATGGGGCAC CTATAGTAGC AAATCAAATA CAAGAGGATA TATTACCCTA TTGCAAACCA 
ACATTCCCCA CTTCAAACAA CCCTCGTGAT CCCAGTTCCT CCAGCTCAAT GTCCTCAAGA 
GGATCTGGTG GACGACGGAG GGGAGAACAA ACAACAGGTC GCAGAAATGC AGCAGAGATG 
CAGCTCTTAG GAAATTATGA ACGAGGGGTT GATGAAGATG AAATAGAGGA AACAGAAAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811710.2
CDS	16..5058
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISEPANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956256.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956256.2

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTGGCG TACCAGCGAG GAGGGGAAGC TGTAAGCAGC 
GCAGGCCGGC CTGGGCTCTT AAATATTTCT GAGCCTGCTA ATCAGCCGTG GCTCGCAGAT 
ACCTGGCCAA ACACTGGAAA CAATCACAAC GAATGCCCCA TCAACTGCTG CACCTCCGGG 
AACGGCAACA GCGACAGCAA CCTCGCCACT TACAGCCGCC CAGCTGACTG CATAGCGAAT 
TACAACAACC AGTTGGACAG CAAGCAGACA AACCTGATGG TGCCCGACTC GGCTGTTTAT 
GGGGATGTAG ACTTAAGCAA CAAGATCAAT GAGATGAAAA CCTTCAACAG CCCCAATCTG 
AAAGATGGGA GGTTTGTCAA TCCTGCCGGC CAGCCCACCC CTTATGCCAC CACTCAGCTC 
ATCCAGTCCA ACATAAGCAA CAACATCAAC ACGTCTGCCG GTGATGTGGA TGAAAAACAC 
TGGAAACCCC CGTGCCAACA GAAACCGGAT GTTACCCCAA TGCAGTACAA CATCATGGAG 
CAAAACAAGA TCAACAAAGA TTACCGAGCT TCGGACACCA TTCCTTCCAC CATTCCCTAC 
AACCAGTCCC ATGATCAGAA TGCCGGAGGG TCATACAACA GCTCAGACCG GGGAAGTAGC 
ACATCTGGGA GCCAAGGTCA TAAGAAGAGT ACTCGTACCC CGAAAGTATC AAAGCAGGCT 
GGCATGAACT GGGCAGATCT TTTGCCACCA CCACCTGCTC ACCCCCCACC ACATGGTGCC 
ACTGAAGACT ACACTTTGTC AGCAGATGAA AGCTATGACA GAGAGTTACC TTGCCCAATA 
CCACCTACAA GAATGTATCT TCAGCAAGAC GAACTGGAAG AGGAAGATGA AAGGGGTCCC 
ACACCTCCTA TTAGAGGAGC AGCTTCTTCT CCGGCTGCTG TGTCCTACAG CCATCAGTCC 
ACTGCCACTC TTACCCCATC ACCTCAGGAC GAACTCCAGC CAATGCTTCA GGACTGTCCC 
GAGGAGATCG GTCATACTCA GCATCAGCTA GAAAGAAGGC GTCATCAAAT TAGTCCTCCT 
CCACCTCCAA GGCCCATTTC CCCGCCTCAT ACATATGGTT ATATTTCTGG ACCAATAGTC 
TCCGATATGG ATACTGATGT TCCTGAAGAG GAAGGGGATG ATGGGGAAAT TGAAGTCACC 
AAGATGCAAA GTCGCAGACT TCTTTTACGT GGCCTGGAGC AGACTCCTGC TTCCAGTGTT 
GGAGACCTAG AAAGTTCTGT GACTGGTTCC ATGATCAATG GATGGGGCTC AGCTTCTGAA 
GAAGACAATG TTTCCAGTGG ACGTTCCAGT GTCAGTTCAT CGGATGGATC ATTTTTCACG 
GATGCGGATT TTGCACAGGC TGTCGCCGCA GCCGCAGAAT ATGCTGGGCT TAAAGTAGCA 
AGGCATCAAA TGCAGGAAGC AGCAGGAGTG TCGCGTCTTT GCCTCCCAGG TCGAAGACCT 
TTCCACGCGT CTCACTGTCC AAGACCAACA AGCCCAGTGT CAACAGACAG CAACATGAGC 
ACCGCTGTCA TACAGAAAAG CCGTCCTGCC AAAAAACAAA AACACCAGCA GGGACATCTT 
CGGAAAGATG TCTACACAGA TGATTTGCCA CCACCGCCAC TGCCACCTCC TGCTATCAAA 
TCTCCAACTG GTCCATCCAA AGCACAACTA GAAGGACGGC CTGGCCTTTC ACAGAAGTAC 
CTTGTTGCAG AACAAAGACT GGAAAGGCCA GGAGAGAGGA AAGGTGTCAG CTACAGGGGC 
AGGGAAGGTC CTGACAGCAG ACAACAACAT GCAGAAGCAA GAACCAATTC AGGGGAGAGA 
AGAGAGTCTT CGGAACAGCA ACTTGATGGA AAGATGAGAA AAGGGAAAGC TTCAAAGTGG 
GATGGGGCAC CTATAGTAGC AAATCAAATA CAAGAGGATA TATTACCCTA TTGCAAACCA 
ACATTCCCCA CTTCAAACAA CCCTCGTGAT CCCAGTTCCT CCAGCTCAAT GTCCTCAAGA 
GGATCTGGTG GACGACGGAG GGGAGAACAA ACAACAGGTC GCAGAAATGC AGCAGAGATG 
CAGCTCTTAG GAAATTATGA ACGAGGGGTT GATGAAGATG AAATAGAGGA AACAGAAAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956258.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811712.2 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42424
Clone ID Related Accession (Same CDS sequence)	XM_012956258.2
Accession Version	XM_012956258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGAGCCTCCG AATTTTGTTG TCAAACCTCG TGACCAAGTT 
GTTGCTCTGG GACGCACAGT CACATTTCAG TGTGAAGCCA CCGGAAACCC CCAGCCAGCC 
ATTTTTTGGC AAAAGGAGGG AAGCCAGAAC CTTCTCTTTT CCTACCAACC TCCACAATCG 
TCGAGCCGCT TCTCCGTGTC CCAGACTGGT GACCTGACTA TAACCACCGT TCAGAAGTCA 
GACGTGGGCT ACTACATCTG CCAGACTCTC AACGTTGCCG GGAGCATTAC AACAAAAGCC 
TTCTTAGAAG TTACCGATGT GGTGGCCGAC CGTCCTCCTC CAGTCATTCG TCAGGGACCC 
GTGAATCAGA CAGTAGCTGT TGATGGCACC TTAATAATGA ACTGTATCGC CAGCGGCATT 
CCTGTTCCCA CAATCCACTG GAGAAAGGAC GGGATCCTGA TACCGACCCA AGACTCTCGC 
ATTAAACAGC AGGACTCAGG AGCTCTGCAG ATCCGTTACG CCAAGCTGAG TGATACCGGT 
CGGTACACCT GCACTGCCTC CACTACTAGC GGGGAAGCAA ACTGGAGCGC GTACATAGAA 
GTCCAAGAAT TTGGAGTTCC GGTTCAACCA CCCAGAGCCA CTGATCCCAA CCTGATTCCA 
AGTGCTCCAT CAAAACCAGA AGTCACGGAC GTCAGCAAAA ATACTGTAAC TTTATCCTGG 
CAGCCAAACC TCAACTCAGG TGCTACTCCT ACGTCGTACC TCATAGAGGC CTTCAGCCAT 
GCGTCTGGAA GCAGCTGGCA AACAGTGGCA GAAAATGTCA AGACCGATTC CTTTGCAGTT 
AAAGCACTGA AGCCCAATGC AATCTACCTC TTCCTCGTGA GAGCAGTAAA CGCATACGGA 
TTAAGTGATC CAAGCCAAAT ATCCGATCCT GTAAAAACAC AAGATATTCC TCCTACAAGT 
CAAGGAGTGG ATCACAAACA GGTGCAGAGA GAACTGGGCG ATGTTGTGCT ACACCTTCAT 
AACCCAACAA TACTTTCCTC TTCCTCCATC GAAGTACATT GGACTGTGGA CCAGCAATCA 
CAGTACATCC AAGGATACAA GATCCTCTAC AGGCCAAGCG CAGAGAATCG AGGCGAATCT 
GCATGGTCCA TATTTGAAGT CAGGACTCCG GCCAAAAACA GCGCAGTTAT TCCTGAACTG 
AGAAAAGGCG TTAACTACGA GATCAAAGCC CGCCCCTTTT TTAATGAGTT TCAAGGAGCT 
GATAGTGAAG TGCGGTTCGC TAAGACAATG GAAGAAGCAC CCAGTGCTCC CCCGCAGAGC 
GTTACTGTAA CCAAGAATGA TGGGAATGGA ACTGCTATTG TTGTGGCATG GCAGCCTCCC 
CCTGAAGATA CTCAGAATGG GATGGTCCAG GAATACAAGG TTTGGTGCCT GGGGAATGAG 
AGTCGCTATC ACATAAACAA GACTGTCGAT GGCTCTACCC TTTCCGTGGT CATCCCGTCC 
TTAGTCCCTG GTATCAAATA CAGCGTGGAG GTGGCAGCCA GCACGGGCGC AGGATCCGGT 
GTGAAAAGTG AACCCAACTA CATCCAGTTA GATTCCTTTG GAAACCCGGT GCCTCCGGGG 
GACCAAGTCA GCCTGGCCCA GCAGATATCC GATGTGGTGA AGCAGCCGGC ATTCATAGCA 
GGGATTGGTG CTGCCTGCTG GATAATCCTC ATGGTATTCA GTATCTGGTT GTACCGGCAC 
AGAAAGAAAC GCAATGGGCT CACCAGTTCC TATGCTGGAA TACGGAAAGT CCCGTCTTTT 
ACCTTCACAC CAACAGTGGC GTACCAGCGA GGAGGGGAAG CTGTAAGCAG CGCAGGCCGG 
CCTGGGCTCT TAAATATTTC TGAGCCTGCT AATCAGCCGT GGCTCGCAGA TACCTGGCCA 
AACACTGGAA ACAATCACAA CGAATGCCCC ATCAACTGCT GCACCTCCGG GAACGGCAAC 
AGCGACAGCA ACCTCGCCAC TTACAGCCGC CCAGCTGACT GCATAGCGAA TTACAACAAC 
CAGTTGGACA GCAAGCAGAC AAACCTGATG GTGCCCGACT CGGCTGTTTA TGGGGATGTA 
GACTTAAGCA ACAAGATCAA TGAGATGAAA ACCTTCAACA GCCCCAATCT GAAAGATGGG 
AGGTTTGTCA ATCCTGCCGG CCAGCCCACC CCTTATGCCA CCACTCAGCT CATCCAGTCC 
AACATAAGCA ACAACATCAA CACGTCTGCC GGTGATGTGG ATGAAAAACA CTGGAAACCC 
CCGTGCCAAC AGAAACCGGA TGTTACCCCA ATGCAGTACA ACATCATGGA GCAAAACAAG 
ATCAACAAAG ATTACCGAGC TTCGGACACC ATTCCTTCCA CCATTCCCTA CAACCAGTCC 
CATGATCAGA ATGCCGGAGG GTCATACAAC AGCTCAGACC GGGGAAGTAG CACATCTGGG 
AGCCAAGGTC ATAAGAAGAG TACTCGTACC CCGAAAGTAT CAAAGCAGGC TGGCATGAAC 
TGGGCAGATC TTTTGCCACC ACCACCTGCT CACCCCCCAC CACATGGTGC CACTGAAGAC 
TACACTTTGT CAGCAGATGA AAGCTATGAC AGAGAGTTAC CTTGCCCAAT ACCACCTACA 
AGAATGTATC TTCAGCAAGA CGAACTGGAA GAGGAAGATG AAAGGGGTCC CACACCTCCT 
ATTAGAGGAG CAGCTTCTTC TCCGGCTGCT GTGTCCTACA GCCATCAGTC CACTGCCACT 
CTTACCCCAT CACCTCAGGA CGAACTCCAG CCAATGCTTC AGGACTGTCC CGAGGAGATC 
GGTCATACTC AGCATCAGCT AGAAAGAAGG CGTCATCAAA TTAGTCCTCC TCCACCTCCA 
AGGCCCATTT CCCCGCCTCA TACATATGGT TATATTTCTG GACCAATAGT CTCCGATATG 
GATACTGATG TTCCTGAAGA GGAAGGGGAT GATGGGGAAA TTGAAGTCAC CAAGATGCAA 
AGTCGCAGAC TTCTTTTACG TGGCCTGGAG CAGACTCCTG CTTCCAGTGT TGGAGACCTA 
GAAAGTTCTG TGACTGGTTC CATGATCAAT GGATGGGGCT CAGCTTCTGA AGAAGACAAT 
GTTTCCAGTG GACGTTCCAG TGTCAGTTCA TCGGATGGAT CATTTTTCAC GGATGCGGAT 
TTTGCACAGG CTGTCGCCGC AGCCGCAGAA TATGCTGGGC TTAAAGTAGC AAGGCATCAA 
ATGCAGGAAG CAGCAGGAGT GTCGCGTCTT TGCCTCCCAG GTCGAAGACC TTTCCACGCG 
TCTCACTGTC CAAGACCAAC AAGCCCAGTG TCAACAGACA GCAACATGAG CACCGCTGTC 
ATACAGAAAA GCCGTCCTGC CAAAAAACAA AAACACCAGC AGGGACATCT TCGGAAAGAT 
GTCTACACAG ATGATTTGCC ACCACCGCCA CTGCCACCTC CTGCTATCAA ATCTCCAACT 
GGTCCATCCA AAGCACAACT AGAAGGACGG CCTGGCCTTT CACAGAAGTA CCTTGTTGCA 
GAACAAAGAC TGGAAAGGCC AGGAGAGAGG AAAGGTGTCA GCTACAGGGG CAGGGAAGGT 
CCTGACAGCA GACAACAACA TGCAGAAGCA AGAACCAATT CAGGGGAGAG AAGAGAGTCT 
TCGGAACAGC AACTTGATGG AAAGATGAGA AAAGGGAAAG CTTCAAAGTG GGATGGGGCA 
CCTATAGTAG CAAATCAAAT ACAAGAGGAT ATATTACCCT ATTGCAAACC AACATTCCCC 
ACTTCAAACA ACCCTCGTGA TCCCAGTTCC TCCAGCTCAA TGTCCTCAAG AGGATCTGGT 
GGACGACGGA GGGGAGAACA AACAACAGGT CGCAGAAATG CAGCAGAGAT GCAGCTCTTA 
GGAAATTATG AACGAGGGGT TGATGAAGAT GAAATAGAGG AAACAGAAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811712.2
CDS	16..5049
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISEPANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956258.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956258.2

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGAGCCTCCG AATTTTGTTG TCAAACCTCG TGACCAAGTT 
GTTGCTCTGG GACGCACAGT CACATTTCAG TGTGAAGCCA CCGGAAACCC CCAGCCAGCC 
ATTTTTTGGC AAAAGGAGGG AAGCCAGAAC CTTCTCTTTT CCTACCAACC TCCACAATCG 
TCGAGCCGCT TCTCCGTGTC CCAGACTGGT GACCTGACTA TAACCACCGT TCAGAAGTCA 
GACGTGGGCT ACTACATCTG CCAGACTCTC AACGTTGCCG GGAGCATTAC AACAAAAGCC 
TTCTTAGAAG TTACCGATGT GGTGGCCGAC CGTCCTCCTC CAGTCATTCG TCAGGGACCC 
GTGAATCAGA CAGTAGCTGT TGATGGCACC TTAATAATGA ACTGTATCGC CAGCGGCATT 
CCTGTTCCCA CAATCCACTG GAGAAAGGAC GGGATCCTGA TACCGACCCA AGACTCTCGC 
ATTAAACAGC AGGACTCAGG AGCTCTGCAG ATCCGTTACG CCAAGCTGAG TGATACCGGT 
CGGTACACCT GCACTGCCTC CACTACTAGC GGGGAAGCAA ACTGGAGCGC GTACATAGAA 
GTCCAAGAAT TTGGAGTTCC GGTTCAACCA CCCAGAGCCA CTGATCCCAA CCTGATTCCA 
AGTGCTCCAT CAAAACCAGA AGTCACGGAC GTCAGCAAAA ATACTGTAAC TTTATCCTGG 
CAGCCAAACC TCAACTCAGG TGCTACTCCT ACGTCGTACC TCATAGAGGC CTTCAGCCAT 
GCGTCTGGAA GCAGCTGGCA AACAGTGGCA GAAAATGTCA AGACCGATTC CTTTGCAGTT 
AAAGCACTGA AGCCCAATGC AATCTACCTC TTCCTCGTGA GAGCAGTAAA CGCATACGGA 
TTAAGTGATC CAAGCCAAAT ATCCGATCCT GTAAAAACAC AAGATATTCC TCCTACAAGT 
CAAGGAGTGG ATCACAAACA GGTGCAGAGA GAACTGGGCG ATGTTGTGCT ACACCTTCAT 
AACCCAACAA TACTTTCCTC TTCCTCCATC GAAGTACATT GGACTGTGGA CCAGCAATCA 
CAGTACATCC AAGGATACAA GATCCTCTAC AGGCCAAGCG CAGAGAATCG AGGCGAATCT 
GCATGGTCCA TATTTGAAGT CAGGACTCCG GCCAAAAACA GCGCAGTTAT TCCTGAACTG 
AGAAAAGGCG TTAACTACGA GATCAAAGCC CGCCCCTTTT TTAATGAGTT TCAAGGAGCT 
GATAGTGAAG TGCGGTTCGC TAAGACAATG GAAGAAGCAC CCAGTGCTCC CCCGCAGAGC 
GTTACTGTAA CCAAGAATGA TGGGAATGGA ACTGCTATTG TTGTGGCATG GCAGCCTCCC 
CCTGAAGATA CTCAGAATGG GATGGTCCAG GAATACAAGG TTTGGTGCCT GGGGAATGAG 
AGTCGCTATC ACATAAACAA GACTGTCGAT GGCTCTACCC TTTCCGTGGT CATCCCGTCC 
TTAGTCCCTG GTATCAAATA CAGCGTGGAG GTGGCAGCCA GCACGGGCGC AGGATCCGGT 
GTGAAAAGTG AACCCAACTA CATCCAGTTA GATTCCTTTG GAAACCCGGT GCCTCCGGGG 
GACCAAGTCA GCCTGGCCCA GCAGATATCC GATGTGGTGA AGCAGCCGGC ATTCATAGCA 
GGGATTGGTG CTGCCTGCTG GATAATCCTC ATGGTATTCA GTATCTGGTT GTACCGGCAC 
AGAAAGAAAC GCAATGGGCT CACCAGTTCC TATGCTGGAA TACGGAAAGT CCCGTCTTTT 
ACCTTCACAC CAACAGTGGC GTACCAGCGA GGAGGGGAAG CTGTAAGCAG CGCAGGCCGG 
CCTGGGCTCT TAAATATTTC TGAGCCTGCT AATCAGCCGT GGCTCGCAGA TACCTGGCCA 
AACACTGGAA ACAATCACAA CGAATGCCCC ATCAACTGCT GCACCTCCGG GAACGGCAAC 
AGCGACAGCA ACCTCGCCAC TTACAGCCGC CCAGCTGACT GCATAGCGAA TTACAACAAC 
CAGTTGGACA GCAAGCAGAC AAACCTGATG GTGCCCGACT CGGCTGTTTA TGGGGATGTA 
GACTTAAGCA ACAAGATCAA TGAGATGAAA ACCTTCAACA GCCCCAATCT GAAAGATGGG 
AGGTTTGTCA ATCCTGCCGG CCAGCCCACC CCTTATGCCA CCACTCAGCT CATCCAGTCC 
AACATAAGCA ACAACATCAA CACGTCTGCC GGTGATGTGG ATGAAAAACA CTGGAAACCC 
CCGTGCCAAC AGAAACCGGA TGTTACCCCA ATGCAGTACA ACATCATGGA GCAAAACAAG 
ATCAACAAAG ATTACCGAGC TTCGGACACC ATTCCTTCCA CCATTCCCTA CAACCAGTCC 
CATGATCAGA ATGCCGGAGG GTCATACAAC AGCTCAGACC GGGGAAGTAG CACATCTGGG 
AGCCAAGGTC ATAAGAAGAG TACTCGTACC CCGAAAGTAT CAAAGCAGGC TGGCATGAAC 
TGGGCAGATC TTTTGCCACC ACCACCTGCT CACCCCCCAC CACATGGTGC CACTGAAGAC 
TACACTTTGT CAGCAGATGA AAGCTATGAC AGAGAGTTAC CTTGCCCAAT ACCACCTACA 
AGAATGTATC TTCAGCAAGA CGAACTGGAA GAGGAAGATG AAAGGGGTCC CACACCTCCT 
ATTAGAGGAG CAGCTTCTTC TCCGGCTGCT GTGTCCTACA GCCATCAGTC CACTGCCACT 
CTTACCCCAT CACCTCAGGA CGAACTCCAG CCAATGCTTC AGGACTGTCC CGAGGAGATC 
GGTCATACTC AGCATCAGCT AGAAAGAAGG CGTCATCAAA TTAGTCCTCC TCCACCTCCA 
AGGCCCATTT CCCCGCCTCA TACATATGGT TATATTTCTG GACCAATAGT CTCCGATATG 
GATACTGATG TTCCTGAAGA GGAAGGGGAT GATGGGGAAA TTGAAGTCAC CAAGATGCAA 
AGTCGCAGAC TTCTTTTACG TGGCCTGGAG CAGACTCCTG CTTCCAGTGT TGGAGACCTA 
GAAAGTTCTG TGACTGGTTC CATGATCAAT GGATGGGGCT CAGCTTCTGA AGAAGACAAT 
GTTTCCAGTG GACGTTCCAG TGTCAGTTCA TCGGATGGAT CATTTTTCAC GGATGCGGAT 
TTTGCACAGG CTGTCGCCGC AGCCGCAGAA TATGCTGGGC TTAAAGTAGC AAGGCATCAA 
ATGCAGGAAG CAGCAGGAGT GTCGCGTCTT TGCCTCCCAG GTCGAAGACC TTTCCACGCG 
TCTCACTGTC CAAGACCAAC AAGCCCAGTG TCAACAGACA GCAACATGAG CACCGCTGTC 
ATACAGAAAA GCCGTCCTGC CAAAAAACAA AAACACCAGC AGGGACATCT TCGGAAAGAT 
GTCTACACAG ATGATTTGCC ACCACCGCCA CTGCCACCTC CTGCTATCAA ATCTCCAACT 
GGTCCATCCA AAGCACAACT AGAAGGACGG CCTGGCCTTT CACAGAAGTA CCTTGTTGCA 
GAACAAAGAC TGGAAAGGCC AGGAGAGAGG AAAGGTGTCA GCTACAGGGG CAGGGAAGGT 
CCTGACAGCA GACAACAACA TGCAGAAGCA AGAACCAATT CAGGGGAGAG AAGAGAGTCT 
TCGGAACAGC AACTTGATGG AAAGATGAGA AAAGGGAAAG CTTCAAAGTG GGATGGGGCA 
CCTATAGTAG CAAATCAAAT ACAAGAGGAT ATATTACCCT ATTGCAAACC AACATTCCCC 
ACTTCAAACA ACCCTCGTGA TCCCAGTTCC TCCAGCTCAA TGTCCTCAAG AGGATCTGGT 
GGACGACGGA GGGGAGAACA AACAACAGGT CGCAGAAATG CAGCAGAGAT GCAGCTCTTA 
GGAAATTATG AACGAGGGGT TGATGAAGAT GAAATAGAGG AAACAGAAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956259.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811713.2 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa42425
Clone ID Related Accession (Same CDS sequence)	XM_012956259.2
Accession Version	XM_012956259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTGGCG TACCAGCGAG GAGGGGAAGC TGTAAGCAGC 
GCAGGCCGGC CTGGGCTCTT AAATATTTCT GAGCCTGCTA ATCAGCCGTG GCTCGCAGAT 
ACCTGGCCAA ACACTGGAAA CAATCACAAC GAATGCCCCA TCAACTGCTG CACCTCCGGG 
AACGGCAACA GCGACAGCAA CCTCGCCACT TACAGCCGCC CAGCTGACTG CATAGCGAAT 
TACAACAACC AGTTGGACAG CAAGCAGACA AACCTGATGG TGCCCGACTC GGCTGTTTAT 
GGGGATGTAG ACTTAAGCAA CAAGATCAAT GAGATGAAAA CCTTCAACAG CCCCAATCTG 
AAAGATGGGA GGTTTGTCAA TCCTGCCGGC CAGCCCACCC CTTATGCCAC CACTCAGCTC 
ATCCAGTCCA ACATAAGCAA CAACATCAAC ACGTCTGCCG GTGATGTGGA TGAAAAACAC 
TGGAAACCCC CGTGCCAACA GAAACCGGAT GTTACCCCAA TGCAGTACAA CATCATGGAG 
CAAAACAAGA TCAACAAAGA TTACCGAGCT TCGGACACCA TTCCTTCCAC CATTCCCTAC 
AACCAGTCCC ATGATCAGAA TGCCGGAGGG TCATACAACA GCTCAGACCG GGGAAGTAGC 
ACATCTGGGA GCCAAGGTCA TAAGAAGAGT ACTCGTACCC CGAAAGTATC AAAGCAGGCT 
GGCATGAACT GGGCAGATCT TTTGCCACCA CCACCTGCTC ACCCCCCACC ACATGGTGCC 
ACTGAAGACT ACACTTTGTC AGCAGATGAA AGCTATGACA GAGAGTTACC TTGCCCAATA 
CCACCTACAA GAATGTATCT TCAGCAAGAC GAACTGGAAG AGGAAGATGA AAGGGGTCCC 
ACACCTCCTA TTAGAGGAGC AGCTTCTTCT CCGGCTGCTG TGTCCTACAG CCATCAGTCC 
ACTGCCACTC TTACCCCATC ACCTCAGGAC GAACTCCAGC CAATGCTTCA GGACTGTCCC 
GAGGAGATCG GTCATACTCA GCATCAGCTA GAAAGAAGGC GTCATCAAAT TAGTCCTCCT 
CCACCTCCAA GGCCCATTTC CCCGCCTCAT ACATATGGTT ATATTTCTGG ACCAATAGTC 
TCCGATATGG ATACTGATGT TCCTGAAGAG GAAGGGGATG ATGGGGAAAT TGAAGTCACC 
AAGATGCAAA GTCGCAGACT TCTTTTACGT GGCCTGGAGC AGACTCCTGC TTCCAGTGTT 
GGAGACCTAG AAAGTTCTGT GACTGGTTCC ATGATCAATG GATGGGGCTC AGCTTCTGAA 
GAAGACAATG TTTCCAGTGG ACGTTCCAGT GTCAGTTCAT CGGATGGATC ATTTTTCACG 
GATGCGGATT TTGCACAGGC TGTCGCCGCA GCCGCAGAAT ATGCTGGGCT TAAAGTAGCA 
AGGCATCAAA TGCAGGAAGC AGCAGGAGGT CGAAGACCTT TCCACGCGTC TCACTGTCCA 
AGACCAACAA GCCCAGTGTC AACAGACAGC AACATGAGCA CCGCTGTCAT ACAGAAAAGC 
CGTCCTGCCA AAAAACAAAA ACACCAGCAG GGACATCTTC GGAAAGATGT CTACACAGAT 
GATTTGCCAC CACCGCCACT GCCACCTCCT GCTATCAAAT CTCCAACTGG TCCATCCAAA 
GCACAACTAG AAGGACGGCC TGGCCTTTCA CAGAAGTACC TTGTTGCAGA ACAAAGACTG 
GAAAGGCCAG GAGAGAGGAA AGGTGTCAGC TACAGGGGCA GGGAAGGTCC TGACAGCAGA 
CAACAACATG CAGAAGCAAG AACCAATTCA GGGGAGAGAA GAGAGTCTTC GGAACAGCAA 
CTTGATGGAA AGATGAGAAA AGGGAAAGCT TCAAAGTGGG ATGGGGCACC TATAGTAGCA 
AATCAAATAC AAGAGGATAT ATTACCCTAT TGCAAACCAA CATTCCCCAC TTCAAACAAC 
CCTCGTGATC CCAGTTCCTC CAGCTCAATG TCCTCAAGAG GATCTGGTGG ACGACGGAGG 
GGAGAACAAA CAACAGGTCG CAGAAATGCA GCAGAGATGC AGCTCTTAGG AAATTATGAA 
CGAGGGGTTG ATGAAGATGA AATAGAGGAA ACAGAAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811713.2
CDS	16..5037
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISEPANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956259.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956259.2

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTGGCG TACCAGCGAG GAGGGGAAGC TGTAAGCAGC 
GCAGGCCGGC CTGGGCTCTT AAATATTTCT GAGCCTGCTA ATCAGCCGTG GCTCGCAGAT 
ACCTGGCCAA ACACTGGAAA CAATCACAAC GAATGCCCCA TCAACTGCTG CACCTCCGGG 
AACGGCAACA GCGACAGCAA CCTCGCCACT TACAGCCGCC CAGCTGACTG CATAGCGAAT 
TACAACAACC AGTTGGACAG CAAGCAGACA AACCTGATGG TGCCCGACTC GGCTGTTTAT 
GGGGATGTAG ACTTAAGCAA CAAGATCAAT GAGATGAAAA CCTTCAACAG CCCCAATCTG 
AAAGATGGGA GGTTTGTCAA TCCTGCCGGC CAGCCCACCC CTTATGCCAC CACTCAGCTC 
ATCCAGTCCA ACATAAGCAA CAACATCAAC ACGTCTGCCG GTGATGTGGA TGAAAAACAC 
TGGAAACCCC CGTGCCAACA GAAACCGGAT GTTACCCCAA TGCAGTACAA CATCATGGAG 
CAAAACAAGA TCAACAAAGA TTACCGAGCT TCGGACACCA TTCCTTCCAC CATTCCCTAC 
AACCAGTCCC ATGATCAGAA TGCCGGAGGG TCATACAACA GCTCAGACCG GGGAAGTAGC 
ACATCTGGGA GCCAAGGTCA TAAGAAGAGT ACTCGTACCC CGAAAGTATC AAAGCAGGCT 
GGCATGAACT GGGCAGATCT TTTGCCACCA CCACCTGCTC ACCCCCCACC ACATGGTGCC 
ACTGAAGACT ACACTTTGTC AGCAGATGAA AGCTATGACA GAGAGTTACC TTGCCCAATA 
CCACCTACAA GAATGTATCT TCAGCAAGAC GAACTGGAAG AGGAAGATGA AAGGGGTCCC 
ACACCTCCTA TTAGAGGAGC AGCTTCTTCT CCGGCTGCTG TGTCCTACAG CCATCAGTCC 
ACTGCCACTC TTACCCCATC ACCTCAGGAC GAACTCCAGC CAATGCTTCA GGACTGTCCC 
GAGGAGATCG GTCATACTCA GCATCAGCTA GAAAGAAGGC GTCATCAAAT TAGTCCTCCT 
CCACCTCCAA GGCCCATTTC CCCGCCTCAT ACATATGGTT ATATTTCTGG ACCAATAGTC 
TCCGATATGG ATACTGATGT TCCTGAAGAG GAAGGGGATG ATGGGGAAAT TGAAGTCACC 
AAGATGCAAA GTCGCAGACT TCTTTTACGT GGCCTGGAGC AGACTCCTGC TTCCAGTGTT 
GGAGACCTAG AAAGTTCTGT GACTGGTTCC ATGATCAATG GATGGGGCTC AGCTTCTGAA 
GAAGACAATG TTTCCAGTGG ACGTTCCAGT GTCAGTTCAT CGGATGGATC ATTTTTCACG 
GATGCGGATT TTGCACAGGC TGTCGCCGCA GCCGCAGAAT ATGCTGGGCT TAAAGTAGCA 
AGGCATCAAA TGCAGGAAGC AGCAGGAGGT CGAAGACCTT TCCACGCGTC TCACTGTCCA 
AGACCAACAA GCCCAGTGTC AACAGACAGC AACATGAGCA CCGCTGTCAT ACAGAAAAGC 
CGTCCTGCCA AAAAACAAAA ACACCAGCAG GGACATCTTC GGAAAGATGT CTACACAGAT 
GATTTGCCAC CACCGCCACT GCCACCTCCT GCTATCAAAT CTCCAACTGG TCCATCCAAA 
GCACAACTAG AAGGACGGCC TGGCCTTTCA CAGAAGTACC TTGTTGCAGA ACAAAGACTG 
GAAAGGCCAG GAGAGAGGAA AGGTGTCAGC TACAGGGGCA GGGAAGGTCC TGACAGCAGA 
CAACAACATG CAGAAGCAAG AACCAATTCA GGGGAGAGAA GAGAGTCTTC GGAACAGCAA 
CTTGATGGAA AGATGAGAAA AGGGAAAGCT TCAAAGTGGG ATGGGGCACC TATAGTAGCA 
AATCAAATAC AAGAGGATAT ATTACCCTAT TGCAAACCAA CATTCCCCAC TTCAAACAAC 
CCTCGTGATC CCAGTTCCTC CAGCTCAATG TCCTCAAGAG GATCTGGTGG ACGACGGAGG 
GGAGAACAAA CAACAGGTCG CAGAAATGCA GCAGAGATGC AGCTCTTAGG AAATTATGAA 
CGAGGGGTTG ATGAAGATGA AATAGAGGAA ACAGAAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956260.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811714.2 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X4, mRNA.

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CloneID	OXa42426
Clone ID Related Accession (Same CDS sequence)	XM_012956260.2
Accession Version	XM_012956260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTG 
GCGTACCAGC GAGGAGGGGA AGCTGTAAGC AGCGCAGGCC GGCCTGGGCT CTTAAATATT 
TCTGAGCCTG CTAATCAGCC GTGGCTCGCA GATACCTGGC CAAACACTGG AAACAATCAC 
AACGAATGCC CCATCAACTG CTGCACCTCC GGGAACGGCA ACAGCGACAG CAACCTCGCC 
ACTTACAGCC GCCCAGCTGA CTGCATAGCG AATTACAACA ACCAGTTGGA CAGCAAGCAG 
ACAAACCTGA TGGTGCCCGA CTCGGCTGTT TATGGGGATG TAGACTTAAG CAACAAGATC 
AATGAGATGA AAACCTTCAA CAGCCCCAAT CTGAAAGATG GGAGGTTTGT CAATCCTGCC 
GGCCAGCCCA CCCCTTATGC CACCACTCAG CTCATCCAGT CCAACATAAG CAACAACATC 
AACACGTCTG CCGGTGATGT GGATGAAAAA CACTGGAAAC CCCCGTGCCA ACAGAAACCG 
GATGTTACCC CAATGCAGTA CAACATCATG GAGCAAAACA AGATCAACAA AGATTACCGA 
GCTTCGGACA CCATTCCTTC CACCATTCCC TACAACCAGT CCCATGATCA GAATGCCGGA 
GGGTCATACA ACAGCTCAGA CCGGGGAAGT AGCACATCTG GGAGCCAAGG TCATAAGAAG 
AGTACTCGTA CCCCGAAAGT ATCAAAGCAG GCTGGCATGA ACTGGGCAGA TCTTTTGCCA 
CCACCACCTG CTCACCCCCC ACCACATGGT GCCACTGAAG ACTACACTTT GTCAGCAGAT 
GAAAGCTATG ACAGAGAGTT ACCTTGCCCA ATACCACCTA CAAGAATGTA TCTTCAGCAA 
GACGAACTGG AAGAGGAAGA TGAAAGGGGT CCCACACCTC CTATTAGAGG AGCAGCTTCT 
TCTCCGGCTG CTGTGTCCTA CAGCCATCAG TCCACTGCCA CTCTTACCCC ATCACCTCAG 
GACGAACTCC AGCCAATGCT TCAGGACTGT CCCGAGGAGA TCGGTCATAC TCAGCATCAG 
CTAGAAAGAA GGCGTCATCA AATTAGTCCT CCTCCACCTC CAAGGCCCAT TTCCCCGCCT 
CATACATATG GTTATATTTC TGGACCAATA GTCTCCGATA TGGATACTGA TGTTCCTGAA 
GAGGAAGGGG ATGATGGGGA AATTGAAGTC ACCAAGATGC AAAGTCGCAG ACTTCTTTTA 
CGTGGCCTGG AGCAGACTCC TGCTTCCAGT GTTGGAGACC TAGAAAGTTC TGTGACTGGT 
TCCATGATCA ATGGATGGGG CTCAGCTTCT GAAGAAGACA ATGTTTCCAG TGGACGTTCC 
AGTGTCAGTT CATCGGATGG ATCATTTTTC ACGGATGCGG ATTTTGCACA GGCTGTCGCC 
GCAGCCGCAG AATATGCTGG GCTTAAAGTA GCAAGGCATC AAATGCAGGA AGCAGCAGGA 
GTGTCGCGTC TTTGCCTCCC AGGTCGAAGA CCTTTCCACG CGTCTCACTG TCCAAGACCA 
ACAAGCCCAG TGTCAACAGA CAGCAACATG AGCACCGCTG TCATACAGAA AAGCCGTCCT 
GCCAAAAAAC AAAAACACCA GCAGGGACAT CTTCGGAAAG ATGTCTACAC AGATGATTTG 
CCACCACCGC CACTGCCACC TCCTGCTATC AAATCTCCAA CTGGTCCATC CAAAGCACAA 
CTAGAAGGAC GGCCTGGCCT TTCACAGAAG TACCTTGTTG CAGAACAAAG ACTGGAAAGG 
CCAGGAGAGA GGAAAGGTGT CAGCTACAGG GGCAGGGAAG GTCCTGACAG CAGACAACAA 
CATGCAGAAG CAAGAACCAA TTCAGGGGAG AGAAGAGAGT CTTCGGAACA GCAACTTGAT 
GGAAAGATGA GAAAAGGGAA AGCTTCAAAG TGGGATGGGG CACCTATAGT AGCAAATCAA 
ATACAAGAGG ATATATTACC CTATTGCAAA CCAACATTCC CCACTTCAAA CAACCCTCGT 
GATCCCAGTT CCTCCAGCTC AATGTCCTCA AGAGGATCTG GTGGACGACG GAGGGGAGAA 
CAAACAACAG GTCGCAGAAA TGCAGCAGAG ATGCAGCTCT TAGGAAATTA TGAACGAGGG 
GTTGATGAAG ATGAAATAGA GGAAACAGAA AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811714.2
CDS	16..5031
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVAYQRGGEAVSSAGRPGLLNISEPANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956260.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956260.2

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTG 
GCGTACCAGC GAGGAGGGGA AGCTGTAAGC AGCGCAGGCC GGCCTGGGCT CTTAAATATT 
TCTGAGCCTG CTAATCAGCC GTGGCTCGCA GATACCTGGC CAAACACTGG AAACAATCAC 
AACGAATGCC CCATCAACTG CTGCACCTCC GGGAACGGCA ACAGCGACAG CAACCTCGCC 
ACTTACAGCC GCCCAGCTGA CTGCATAGCG AATTACAACA ACCAGTTGGA CAGCAAGCAG 
ACAAACCTGA TGGTGCCCGA CTCGGCTGTT TATGGGGATG TAGACTTAAG CAACAAGATC 
AATGAGATGA AAACCTTCAA CAGCCCCAAT CTGAAAGATG GGAGGTTTGT CAATCCTGCC 
GGCCAGCCCA CCCCTTATGC CACCACTCAG CTCATCCAGT CCAACATAAG CAACAACATC 
AACACGTCTG CCGGTGATGT GGATGAAAAA CACTGGAAAC CCCCGTGCCA ACAGAAACCG 
GATGTTACCC CAATGCAGTA CAACATCATG GAGCAAAACA AGATCAACAA AGATTACCGA 
GCTTCGGACA CCATTCCTTC CACCATTCCC TACAACCAGT CCCATGATCA GAATGCCGGA 
GGGTCATACA ACAGCTCAGA CCGGGGAAGT AGCACATCTG GGAGCCAAGG TCATAAGAAG 
AGTACTCGTA CCCCGAAAGT ATCAAAGCAG GCTGGCATGA ACTGGGCAGA TCTTTTGCCA 
CCACCACCTG CTCACCCCCC ACCACATGGT GCCACTGAAG ACTACACTTT GTCAGCAGAT 
GAAAGCTATG ACAGAGAGTT ACCTTGCCCA ATACCACCTA CAAGAATGTA TCTTCAGCAA 
GACGAACTGG AAGAGGAAGA TGAAAGGGGT CCCACACCTC CTATTAGAGG AGCAGCTTCT 
TCTCCGGCTG CTGTGTCCTA CAGCCATCAG TCCACTGCCA CTCTTACCCC ATCACCTCAG 
GACGAACTCC AGCCAATGCT TCAGGACTGT CCCGAGGAGA TCGGTCATAC TCAGCATCAG 
CTAGAAAGAA GGCGTCATCA AATTAGTCCT CCTCCACCTC CAAGGCCCAT TTCCCCGCCT 
CATACATATG GTTATATTTC TGGACCAATA GTCTCCGATA TGGATACTGA TGTTCCTGAA 
GAGGAAGGGG ATGATGGGGA AATTGAAGTC ACCAAGATGC AAAGTCGCAG ACTTCTTTTA 
CGTGGCCTGG AGCAGACTCC TGCTTCCAGT GTTGGAGACC TAGAAAGTTC TGTGACTGGT 
TCCATGATCA ATGGATGGGG CTCAGCTTCT GAAGAAGACA ATGTTTCCAG TGGACGTTCC 
AGTGTCAGTT CATCGGATGG ATCATTTTTC ACGGATGCGG ATTTTGCACA GGCTGTCGCC 
GCAGCCGCAG AATATGCTGG GCTTAAAGTA GCAAGGCATC AAATGCAGGA AGCAGCAGGA 
GTGTCGCGTC TTTGCCTCCC AGGTCGAAGA CCTTTCCACG CGTCTCACTG TCCAAGACCA 
ACAAGCCCAG TGTCAACAGA CAGCAACATG AGCACCGCTG TCATACAGAA AAGCCGTCCT 
GCCAAAAAAC AAAAACACCA GCAGGGACAT CTTCGGAAAG ATGTCTACAC AGATGATTTG 
CCACCACCGC CACTGCCACC TCCTGCTATC AAATCTCCAA CTGGTCCATC CAAAGCACAA 
CTAGAAGGAC GGCCTGGCCT TTCACAGAAG TACCTTGTTG CAGAACAAAG ACTGGAAAGG 
CCAGGAGAGA GGAAAGGTGT CAGCTACAGG GGCAGGGAAG GTCCTGACAG CAGACAACAA 
CATGCAGAAG CAAGAACCAA TTCAGGGGAG AGAAGAGAGT CTTCGGAACA GCAACTTGAT 
GGAAAGATGA GAAAAGGGAA AGCTTCAAAG TGGGATGGGG CACCTATAGT AGCAAATCAA 
ATACAAGAGG ATATATTACC CTATTGCAAA CCAACATTCC CCACTTCAAA CAACCCTCGT 
GATCCCAGTT CCTCCAGCTC AATGTCCTCA AGAGGATCTG GTGGACGACG GAGGGGAGAA 
CAAACAACAG GTCGCAGAAA TGCAGCAGAG ATGCAGCTCT TAGGAAATTA TGAACGAGGG 
GTTGATGAAG ATGAAATAGA GGAAACAGAA AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956261.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811715.2 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X5, mRNA.

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CloneID	OXa42427
Clone ID Related Accession (Same CDS sequence)	XM_012956261.2
Accession Version	XM_012956261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGAGCCTCCG AATTTTGTTG TCAAACCTCG TGACCAAGTT 
GTTGCTCTGG GACGCACAGT CACATTTCAG TGTGAAGCCA CCGGAAACCC CCAGCCAGCC 
ATTTTTTGGC AAAAGGAGGG AAGCCAGAAC CTTCTCTTTT CCTACCAACC TCCACAATCG 
TCGAGCCGCT TCTCCGTGTC CCAGACTGGT GACCTGACTA TAACCACCGT TCAGAAGTCA 
GACGTGGGCT ACTACATCTG CCAGACTCTC AACGTTGCCG GGAGCATTAC AACAAAAGCC 
TTCTTAGAAG TTACCGATGT GGTGGCCGAC CGTCCTCCTC CAGTCATTCG TCAGGGACCC 
GTGAATCAGA CAGTAGCTGT TGATGGCACC TTAATAATGA ACTGTATCGC CAGCGGCATT 
CCTGTTCCCA CAATCCACTG GAGAAAGGAC GGGATCCTGA TACCGACCCA AGACTCTCGC 
ATTAAACAGC AGGACTCAGG AGCTCTGCAG ATCCGTTACG CCAAGCTGAG TGATACCGGT 
CGGTACACCT GCACTGCCTC CACTACTAGC GGGGAAGCAA ACTGGAGCGC GTACATAGAA 
GTCCAAGAAT TTGGAGTTCC GGTTCAACCA CCCAGAGCCA CTGATCCCAA CCTGATTCCA 
AGTGCTCCAT CAAAACCAGA AGTCACGGAC GTCAGCAAAA ATACTGTAAC TTTATCCTGG 
CAGCCAAACC TCAACTCAGG TGCTACTCCT ACGTCGTACC TCATAGAGGC CTTCAGCCAT 
GCGTCTGGAA GCAGCTGGCA AACAGTGGCA GAAAATGTCA AGACCGATTC CTTTGCAGTT 
AAAGCACTGA AGCCCAATGC AATCTACCTC TTCCTCGTGA GAGCAGTAAA CGCATACGGA 
TTAAGTGATC CAAGCCAAAT ATCCGATCCT GTAAAAACAC AAGATATTCC TCCTACAAGT 
CAAGGAGTGG ATCACAAACA GGTGCAGAGA GAACTGGGCG ATGTTGTGCT ACACCTTCAT 
AACCCAACAA TACTTTCCTC TTCCTCCATC GAAGTACATT GGACTGTGGA CCAGCAATCA 
CAGTACATCC AAGGATACAA GATCCTCTAC AGGCCAAGCG CAGAGAATCG AGGCGAATCT 
GCATGGTCCA TATTTGAAGT CAGGACTCCG GCCAAAAACA GCGCAGTTAT TCCTGAACTG 
AGAAAAGGCG TTAACTACGA GATCAAAGCC CGCCCCTTTT TTAATGAGTT TCAAGGAGCT 
GATAGTGAAG TGCGGTTCGC TAAGACAATG GAAGAAGCAC CCAGTGCTCC CCCGCAGAGC 
GTTACTGTAA CCAAGAATGA TGGGAATGGA ACTGCTATTG TTGTGGCATG GCAGCCTCCC 
CCTGAAGATA CTCAGAATGG GATGGTCCAG GAATACAAGG TTTGGTGCCT GGGGAATGAG 
AGTCGCTATC ACATAAACAA GACTGTCGAT GGCTCTACCC TTTCCGTGGT CATCCCGTCC 
TTAGTCCCTG GTATCAAATA CAGCGTGGAG GTGGCAGCCA GCACGGGCGC AGGATCCGGT 
GTGAAAAGTG AACCCAACTA CATCCAGTTA GATTCCTTTG GAAACCCGGT GCCTCCGGGG 
GACCAAGTCA GCCTGGCCCA GCAGATATCC GATGTGGTGA AGCAGCCGGC ATTCATAGCA 
GGGATTGGTG CTGCCTGCTG GATAATCCTC ATGGTATTCA GTATCTGGTT GTACCGGCAC 
AGAAAGAAAC GCAATGGGCT CACCAGTTCC TATGCTGGAA TACGGAAAGT CCCGTCTTTT 
ACCTTCACAC CAACAGTGGC GTACCAGCGA GGAGGGGAAG CTGTAAGCAG CGCAGGCCGG 
CCTGGGCTCT TAAATATTTC TGAGCCTGCT AATCAGCCGT GGCTCGCAGA TACCTGGCCA 
AACACTGGAA ACAATCACAA CGAATGCCCC ATCAACTGCT GCACCTCCGG GAACGGCAAC 
AGCGACAGCA ACCTCGCCAC TTACAGCCGC CCAGCTGACT GCATAGCGAA TTACAACAAC 
CAGTTGGACA GCAAGCAGAC AAACCTGATG GTGCCCGACT CGGCTGTTTA TGGGGATGTA 
GACTTAAGCA ACAAGATCAA TGAGATGAAA ACCTTCAACA GCCCCAATCT GAAAGATGGG 
AGGTTTGTCA ATCCTGCCGG CCAGCCCACC CCTTATGCCA CCACTCAGCT CATCCAGTCC 
AACATAAGCA ACAACATCAA CACGTCTGCC GGTGATGTGG ATGAAAAACA CTGGAAACCC 
CCGTGCCAAC AGAAACCGGA TGTTACCCCA ATGCAGTACA ACATCATGGA GCAAAACAAG 
ATCAACAAAG ATTACCGAGC TTCGGACACC ATTCCTTCCA CCATTCCCTA CAACCAGTCC 
CATGATCAGA ATGCCGGAGG GTCATACAAC AGCTCAGACC GGGGAAGTAG CACATCTGGG 
AGCCAAGGTC ATAAGAAGAG TACTCGTACC CCGAAAGTAT CAAAGCAGGC TGGCATGAAC 
TGGGCAGATC TTTTGCCACC ACCACCTGCT CACCCCCCAC CACATGGTGC CACTGAAGAC 
TACACTTTGT CAGCAGATGA AAGCTATGAC AGAGAGTTAC CTTGCCCAAT ACCACCTACA 
AGAATGTATC TTCAGCAAGA CGAACTGGAA GAGGAAGATG AAAGGGGTCC CACACCTCCT 
ATTAGAGGAG CAGCTTCTTC TCCGGCTGCT GTGTCCTACA GCCATCAGTC CACTGCCACT 
CTTACCCCAT CACCTCAGGA CGAACTCCAG CCAATGCTTC AGGACTGTCC CGAGGAGATC 
GGTCATACTC AGCATCAGCT AGAAAGAAGG CGTCATCAAA TTAGTCCTCC TCCACCTCCA 
AGGCCCATTT CCCCGCCTCA TACATATGGT TATATTTCTG GACCAATAGT CTCCGATATG 
GATACTGATG TTCCTGAAGA GGAAGGGGAT GATGGGGAAA TTGAAGTCAC CAAGATGCAA 
AGTCGCAGAC TTCTTTTACG TGGCCTGGAG CAGACTCCTG CTTCCAGTGT TGGAGACCTA 
GAAAGTTCTG TGACTGGTTC CATGATCAAT GGATGGGGCT CAGCTTCTGA AGAAGACAAT 
GTTTCCAGTG GACGTTCCAG TGTCAGTTCA TCGGATGGAT CATTTTTCAC GGATGCGGAT 
TTTGCACAGG CTGTCGCCGC AGCCGCAGAA TATGCTGGGC TTAAAGTAGC AAGGCATCAA 
ATGCAGGAAG CAGCAGGAGG TCGAAGACCT TTCCACGCGT CTCACTGTCC AAGACCAACA 
AGCCCAGTGT CAACAGACAG CAACATGAGC ACCGCTGTCA TACAGAAAAG CCGTCCTGCC 
AAAAAACAAA AACACCAGCA GGGACATCTT CGGAAAGATG TCTACACAGA TGATTTGCCA 
CCACCGCCAC TGCCACCTCC TGCTATCAAA TCTCCAACTG GTCCATCCAA AGCACAACTA 
GAAGGACGGC CTGGCCTTTC ACAGAAGTAC CTTGTTGCAG AACAAAGACT GGAAAGGCCA 
GGAGAGAGGA AAGGTGTCAG CTACAGGGGC AGGGAAGGTC CTGACAGCAG ACAACAACAT 
GCAGAAGCAA GAACCAATTC AGGGGAGAGA AGAGAGTCTT CGGAACAGCA ACTTGATGGA 
AAGATGAGAA AAGGGAAAGC TTCAAAGTGG GATGGGGCAC CTATAGTAGC AAATCAAATA 
CAAGAGGATA TATTACCCTA TTGCAAACCA ACATTCCCCA CTTCAAACAA CCCTCGTGAT 
CCCAGTTCCT CCAGCTCAAT GTCCTCAAGA GGATCTGGTG GACGACGGAG GGGAGAACAA 
ACAACAGGTC GCAGAAATGC AGCAGAGATG CAGCTCTTAG GAAATTATGA ACGAGGGGTT 
GATGAAGATG AAATAGAGGA AACAGAAAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811715.2
CDS	16..5028
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISEPANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956261.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956261.2

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGAGCCTCCG AATTTTGTTG TCAAACCTCG TGACCAAGTT 
GTTGCTCTGG GACGCACAGT CACATTTCAG TGTGAAGCCA CCGGAAACCC CCAGCCAGCC 
ATTTTTTGGC AAAAGGAGGG AAGCCAGAAC CTTCTCTTTT CCTACCAACC TCCACAATCG 
TCGAGCCGCT TCTCCGTGTC CCAGACTGGT GACCTGACTA TAACCACCGT TCAGAAGTCA 
GACGTGGGCT ACTACATCTG CCAGACTCTC AACGTTGCCG GGAGCATTAC AACAAAAGCC 
TTCTTAGAAG TTACCGATGT GGTGGCCGAC CGTCCTCCTC CAGTCATTCG TCAGGGACCC 
GTGAATCAGA CAGTAGCTGT TGATGGCACC TTAATAATGA ACTGTATCGC CAGCGGCATT 
CCTGTTCCCA CAATCCACTG GAGAAAGGAC GGGATCCTGA TACCGACCCA AGACTCTCGC 
ATTAAACAGC AGGACTCAGG AGCTCTGCAG ATCCGTTACG CCAAGCTGAG TGATACCGGT 
CGGTACACCT GCACTGCCTC CACTACTAGC GGGGAAGCAA ACTGGAGCGC GTACATAGAA 
GTCCAAGAAT TTGGAGTTCC GGTTCAACCA CCCAGAGCCA CTGATCCCAA CCTGATTCCA 
AGTGCTCCAT CAAAACCAGA AGTCACGGAC GTCAGCAAAA ATACTGTAAC TTTATCCTGG 
CAGCCAAACC TCAACTCAGG TGCTACTCCT ACGTCGTACC TCATAGAGGC CTTCAGCCAT 
GCGTCTGGAA GCAGCTGGCA AACAGTGGCA GAAAATGTCA AGACCGATTC CTTTGCAGTT 
AAAGCACTGA AGCCCAATGC AATCTACCTC TTCCTCGTGA GAGCAGTAAA CGCATACGGA 
TTAAGTGATC CAAGCCAAAT ATCCGATCCT GTAAAAACAC AAGATATTCC TCCTACAAGT 
CAAGGAGTGG ATCACAAACA GGTGCAGAGA GAACTGGGCG ATGTTGTGCT ACACCTTCAT 
AACCCAACAA TACTTTCCTC TTCCTCCATC GAAGTACATT GGACTGTGGA CCAGCAATCA 
CAGTACATCC AAGGATACAA GATCCTCTAC AGGCCAAGCG CAGAGAATCG AGGCGAATCT 
GCATGGTCCA TATTTGAAGT CAGGACTCCG GCCAAAAACA GCGCAGTTAT TCCTGAACTG 
AGAAAAGGCG TTAACTACGA GATCAAAGCC CGCCCCTTTT TTAATGAGTT TCAAGGAGCT 
GATAGTGAAG TGCGGTTCGC TAAGACAATG GAAGAAGCAC CCAGTGCTCC CCCGCAGAGC 
GTTACTGTAA CCAAGAATGA TGGGAATGGA ACTGCTATTG TTGTGGCATG GCAGCCTCCC 
CCTGAAGATA CTCAGAATGG GATGGTCCAG GAATACAAGG TTTGGTGCCT GGGGAATGAG 
AGTCGCTATC ACATAAACAA GACTGTCGAT GGCTCTACCC TTTCCGTGGT CATCCCGTCC 
TTAGTCCCTG GTATCAAATA CAGCGTGGAG GTGGCAGCCA GCACGGGCGC AGGATCCGGT 
GTGAAAAGTG AACCCAACTA CATCCAGTTA GATTCCTTTG GAAACCCGGT GCCTCCGGGG 
GACCAAGTCA GCCTGGCCCA GCAGATATCC GATGTGGTGA AGCAGCCGGC ATTCATAGCA 
GGGATTGGTG CTGCCTGCTG GATAATCCTC ATGGTATTCA GTATCTGGTT GTACCGGCAC 
AGAAAGAAAC GCAATGGGCT CACCAGTTCC TATGCTGGAA TACGGAAAGT CCCGTCTTTT 
ACCTTCACAC CAACAGTGGC GTACCAGCGA GGAGGGGAAG CTGTAAGCAG CGCAGGCCGG 
CCTGGGCTCT TAAATATTTC TGAGCCTGCT AATCAGCCGT GGCTCGCAGA TACCTGGCCA 
AACACTGGAA ACAATCACAA CGAATGCCCC ATCAACTGCT GCACCTCCGG GAACGGCAAC 
AGCGACAGCA ACCTCGCCAC TTACAGCCGC CCAGCTGACT GCATAGCGAA TTACAACAAC 
CAGTTGGACA GCAAGCAGAC AAACCTGATG GTGCCCGACT CGGCTGTTTA TGGGGATGTA 
GACTTAAGCA ACAAGATCAA TGAGATGAAA ACCTTCAACA GCCCCAATCT GAAAGATGGG 
AGGTTTGTCA ATCCTGCCGG CCAGCCCACC CCTTATGCCA CCACTCAGCT CATCCAGTCC 
AACATAAGCA ACAACATCAA CACGTCTGCC GGTGATGTGG ATGAAAAACA CTGGAAACCC 
CCGTGCCAAC AGAAACCGGA TGTTACCCCA ATGCAGTACA ACATCATGGA GCAAAACAAG 
ATCAACAAAG ATTACCGAGC TTCGGACACC ATTCCTTCCA CCATTCCCTA CAACCAGTCC 
CATGATCAGA ATGCCGGAGG GTCATACAAC AGCTCAGACC GGGGAAGTAG CACATCTGGG 
AGCCAAGGTC ATAAGAAGAG TACTCGTACC CCGAAAGTAT CAAAGCAGGC TGGCATGAAC 
TGGGCAGATC TTTTGCCACC ACCACCTGCT CACCCCCCAC CACATGGTGC CACTGAAGAC 
TACACTTTGT CAGCAGATGA AAGCTATGAC AGAGAGTTAC CTTGCCCAAT ACCACCTACA 
AGAATGTATC TTCAGCAAGA CGAACTGGAA GAGGAAGATG AAAGGGGTCC CACACCTCCT 
ATTAGAGGAG CAGCTTCTTC TCCGGCTGCT GTGTCCTACA GCCATCAGTC CACTGCCACT 
CTTACCCCAT CACCTCAGGA CGAACTCCAG CCAATGCTTC AGGACTGTCC CGAGGAGATC 
GGTCATACTC AGCATCAGCT AGAAAGAAGG CGTCATCAAA TTAGTCCTCC TCCACCTCCA 
AGGCCCATTT CCCCGCCTCA TACATATGGT TATATTTCTG GACCAATAGT CTCCGATATG 
GATACTGATG TTCCTGAAGA GGAAGGGGAT GATGGGGAAA TTGAAGTCAC CAAGATGCAA 
AGTCGCAGAC TTCTTTTACG TGGCCTGGAG CAGACTCCTG CTTCCAGTGT TGGAGACCTA 
GAAAGTTCTG TGACTGGTTC CATGATCAAT GGATGGGGCT CAGCTTCTGA AGAAGACAAT 
GTTTCCAGTG GACGTTCCAG TGTCAGTTCA TCGGATGGAT CATTTTTCAC GGATGCGGAT 
TTTGCACAGG CTGTCGCCGC AGCCGCAGAA TATGCTGGGC TTAAAGTAGC AAGGCATCAA 
ATGCAGGAAG CAGCAGGAGG TCGAAGACCT TTCCACGCGT CTCACTGTCC AAGACCAACA 
AGCCCAGTGT CAACAGACAG CAACATGAGC ACCGCTGTCA TACAGAAAAG CCGTCCTGCC 
AAAAAACAAA AACACCAGCA GGGACATCTT CGGAAAGATG TCTACACAGA TGATTTGCCA 
CCACCGCCAC TGCCACCTCC TGCTATCAAA TCTCCAACTG GTCCATCCAA AGCACAACTA 
GAAGGACGGC CTGGCCTTTC ACAGAAGTAC CTTGTTGCAG AACAAAGACT GGAAAGGCCA 
GGAGAGAGGA AAGGTGTCAG CTACAGGGGC AGGGAAGGTC CTGACAGCAG ACAACAACAT 
GCAGAAGCAA GAACCAATTC AGGGGAGAGA AGAGAGTCTT CGGAACAGCA ACTTGATGGA 
AAGATGAGAA AAGGGAAAGC TTCAAAGTGG GATGGGGCAC CTATAGTAGC AAATCAAATA 
CAAGAGGATA TATTACCCTA TTGCAAACCA ACATTCCCCA CTTCAAACAA CCCTCGTGAT 
CCCAGTTCCT CCAGCTCAAT GTCCTCAAGA GGATCTGGTG GACGACGGAG GGGAGAACAA 
ACAACAGGTC GCAGAAATGC AGCAGAGATG CAGCTCTTAG GAAATTATGA ACGAGGGGTT 
GATGAAGATG AAATAGAGGA AACAGAAAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956262.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811716.2 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X6, mRNA.

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CloneID	OXa42428
Clone ID Related Accession (Same CDS sequence)	XM_012956262.2
Accession Version	XM_012956262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTG 
GCGTACCAGC GAGGAGGGGA AGCTGTAAGC AGCGCAGGCC GGCCTGGGCT CTTAAATATT 
TCTGAGCCTG CTAATCAGCC GTGGCTCGCA GATACCTGGC CAAACACTGG AAACAATCAC 
AACGAATGCC CCATCAACTG CTGCACCTCC GGGAACGGCA ACAGCGACAG CAACCTCGCC 
ACTTACAGCC GCCCAGCTGA CTGCATAGCG AATTACAACA ACCAGTTGGA CAGCAAGCAG 
ACAAACCTGA TGGTGCCCGA CTCGGCTGTT TATGGGGATG TAGACTTAAG CAACAAGATC 
AATGAGATGA AAACCTTCAA CAGCCCCAAT CTGAAAGATG GGAGGTTTGT CAATCCTGCC 
GGCCAGCCCA CCCCTTATGC CACCACTCAG CTCATCCAGT CCAACATAAG CAACAACATC 
AACACGTCTG CCGGTGATGT GGATGAAAAA CACTGGAAAC CCCCGTGCCA ACAGAAACCG 
GATGTTACCC CAATGCAGTA CAACATCATG GAGCAAAACA AGATCAACAA AGATTACCGA 
GCTTCGGACA CCATTCCTTC CACCATTCCC TACAACCAGT CCCATGATCA GAATGCCGGA 
GGGTCATACA ACAGCTCAGA CCGGGGAAGT AGCACATCTG GGAGCCAAGG TCATAAGAAG 
AGTACTCGTA CCCCGAAAGT ATCAAAGCAG GCTGGCATGA ACTGGGCAGA TCTTTTGCCA 
CCACCACCTG CTCACCCCCC ACCACATGGT GCCACTGAAG ACTACACTTT GTCAGCAGAT 
GAAAGCTATG ACAGAGAGTT ACCTTGCCCA ATACCACCTA CAAGAATGTA TCTTCAGCAA 
GACGAACTGG AAGAGGAAGA TGAAAGGGGT CCCACACCTC CTATTAGAGG AGCAGCTTCT 
TCTCCGGCTG CTGTGTCCTA CAGCCATCAG TCCACTGCCA CTCTTACCCC ATCACCTCAG 
GACGAACTCC AGCCAATGCT TCAGGACTGT CCCGAGGAGA TCGGTCATAC TCAGCATCAG 
CTAGAAAGAA GGCGTCATCA AATTAGTCCT CCTCCACCTC CAAGGCCCAT TTCCCCGCCT 
CATACATATG GTTATATTTC TGGACCAATA GTCTCCGATA TGGATACTGA TGTTCCTGAA 
GAGGAAGGGG ATGATGGGGA AATTGAAGTC ACCAAGATGC AAAGTCGCAG ACTTCTTTTA 
CGTGGCCTGG AGCAGACTCC TGCTTCCAGT GTTGGAGACC TAGAAAGTTC TGTGACTGGT 
TCCATGATCA ATGGATGGGG CTCAGCTTCT GAAGAAGACA ATGTTTCCAG TGGACGTTCC 
AGTGTCAGTT CATCGGATGG ATCATTTTTC ACGGATGCGG ATTTTGCACA GGCTGTCGCC 
GCAGCCGCAG AATATGCTGG GCTTAAAGTA GCAAGGCATC AAATGCAGGA AGCAGCAGGA 
GGTCGAAGAC CTTTCCACGC GTCTCACTGT CCAAGACCAA CAAGCCCAGT GTCAACAGAC 
AGCAACATGA GCACCGCTGT CATACAGAAA AGCCGTCCTG CCAAAAAACA AAAACACCAG 
CAGGGACATC TTCGGAAAGA TGTCTACACA GATGATTTGC CACCACCGCC ACTGCCACCT 
CCTGCTATCA AATCTCCAAC TGGTCCATCC AAAGCACAAC TAGAAGGACG GCCTGGCCTT 
TCACAGAAGT ACCTTGTTGC AGAACAAAGA CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC 
AGCTACAGGG GCAGGGAAGG TCCTGACAGC AGACAACAAC ATGCAGAAGC AAGAACCAAT 
TCAGGGGAGA GAAGAGAGTC TTCGGAACAG CAACTTGATG GAAAGATGAG AAAAGGGAAA 
GCTTCAAAGT GGGATGGGGC ACCTATAGTA GCAAATCAAA TACAAGAGGA TATATTACCC 
TATTGCAAAC CAACATTCCC CACTTCAAAC AACCCTCGTG ATCCCAGTTC CTCCAGCTCA 
ATGTCCTCAA GAGGATCTGG TGGACGACGG AGGGGAGAAC AAACAACAGG TCGCAGAAAT 
GCAGCAGAGA TGCAGCTCTT AGGAAATTAT GAACGAGGGG TTGATGAAGA TGAAATAGAG 
GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811716.2
CDS	16..5010
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVAYQRGGEAVSSAGRPGLLNISEPANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956262.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956262.2

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTCGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTG 
GCGTACCAGC GAGGAGGGGA AGCTGTAAGC AGCGCAGGCC GGCCTGGGCT CTTAAATATT 
TCTGAGCCTG CTAATCAGCC GTGGCTCGCA GATACCTGGC CAAACACTGG AAACAATCAC 
AACGAATGCC CCATCAACTG CTGCACCTCC GGGAACGGCA ACAGCGACAG CAACCTCGCC 
ACTTACAGCC GCCCAGCTGA CTGCATAGCG AATTACAACA ACCAGTTGGA CAGCAAGCAG 
ACAAACCTGA TGGTGCCCGA CTCGGCTGTT TATGGGGATG TAGACTTAAG CAACAAGATC 
AATGAGATGA AAACCTTCAA CAGCCCCAAT CTGAAAGATG GGAGGTTTGT CAATCCTGCC 
GGCCAGCCCA CCCCTTATGC CACCACTCAG CTCATCCAGT CCAACATAAG CAACAACATC 
AACACGTCTG CCGGTGATGT GGATGAAAAA CACTGGAAAC CCCCGTGCCA ACAGAAACCG 
GATGTTACCC CAATGCAGTA CAACATCATG GAGCAAAACA AGATCAACAA AGATTACCGA 
GCTTCGGACA CCATTCCTTC CACCATTCCC TACAACCAGT CCCATGATCA GAATGCCGGA 
GGGTCATACA ACAGCTCAGA CCGGGGAAGT AGCACATCTG GGAGCCAAGG TCATAAGAAG 
AGTACTCGTA CCCCGAAAGT ATCAAAGCAG GCTGGCATGA ACTGGGCAGA TCTTTTGCCA 
CCACCACCTG CTCACCCCCC ACCACATGGT GCCACTGAAG ACTACACTTT GTCAGCAGAT 
GAAAGCTATG ACAGAGAGTT ACCTTGCCCA ATACCACCTA CAAGAATGTA TCTTCAGCAA 
GACGAACTGG AAGAGGAAGA TGAAAGGGGT CCCACACCTC CTATTAGAGG AGCAGCTTCT 
TCTCCGGCTG CTGTGTCCTA CAGCCATCAG TCCACTGCCA CTCTTACCCC ATCACCTCAG 
GACGAACTCC AGCCAATGCT TCAGGACTGT CCCGAGGAGA TCGGTCATAC TCAGCATCAG 
CTAGAAAGAA GGCGTCATCA AATTAGTCCT CCTCCACCTC CAAGGCCCAT TTCCCCGCCT 
CATACATATG GTTATATTTC TGGACCAATA GTCTCCGATA TGGATACTGA TGTTCCTGAA 
GAGGAAGGGG ATGATGGGGA AATTGAAGTC ACCAAGATGC AAAGTCGCAG ACTTCTTTTA 
CGTGGCCTGG AGCAGACTCC TGCTTCCAGT GTTGGAGACC TAGAAAGTTC TGTGACTGGT 
TCCATGATCA ATGGATGGGG CTCAGCTTCT GAAGAAGACA ATGTTTCCAG TGGACGTTCC 
AGTGTCAGTT CATCGGATGG ATCATTTTTC ACGGATGCGG ATTTTGCACA GGCTGTCGCC 
GCAGCCGCAG AATATGCTGG GCTTAAAGTA GCAAGGCATC AAATGCAGGA AGCAGCAGGA 
GGTCGAAGAC CTTTCCACGC GTCTCACTGT CCAAGACCAA CAAGCCCAGT GTCAACAGAC 
AGCAACATGA GCACCGCTGT CATACAGAAA AGCCGTCCTG CCAAAAAACA AAAACACCAG 
CAGGGACATC TTCGGAAAGA TGTCTACACA GATGATTTGC CACCACCGCC ACTGCCACCT 
CCTGCTATCA AATCTCCAAC TGGTCCATCC AAAGCACAAC TAGAAGGACG GCCTGGCCTT 
TCACAGAAGT ACCTTGTTGC AGAACAAAGA CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC 
AGCTACAGGG GCAGGGAAGG TCCTGACAGC AGACAACAAC ATGCAGAAGC AAGAACCAAT 
TCAGGGGAGA GAAGAGAGTC TTCGGAACAG CAACTTGATG GAAAGATGAG AAAAGGGAAA 
GCTTCAAAGT GGGATGGGGC ACCTATAGTA GCAAATCAAA TACAAGAGGA TATATTACCC 
TATTGCAAAC CAACATTCCC CACTTCAAAC AACCCTCGTG ATCCCAGTTC CTCCAGCTCA 
ATGTCCTCAA GAGGATCTGG TGGACGACGG AGGGGAGAAC AAACAACAGG TCGCAGAAAT 
GCAGCAGAGA TGCAGCTCTT AGGAAATTAT GAACGAGGGG TTGATGAAGA TGAAATAGAG 
GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956256.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811710.3 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X1, mRNA.

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CloneID	OXa51062
Clone ID Related Accession (Same CDS sequence)	XM_012956256.3
Accession Version	XM_012956256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTGGCG TACCAGCGAG GAGGGGAAGC TGTAAGCAGC 
GCAGGCCGGC CTGGGCTCTT AAATATTTCT GAGACTGCTA ATCAGCCGTG GCTCGCAGAT 
ACCTGGCCAA ACACTGGAAA CAATCACAAC GAATGCCCCA TCAACTGCTG CACCTCCGGG 
AATGGCAACA GCGACAGCAA CCTCGCCACT TACAGCCGCC CAGCTGACTG CATAGCGAAT 
TACAACAACC AGTTGGACAG CAAGCAGACA AACCTGATGG TGCCCGACTC GGCTGTTTAT 
GGGGATGTAG ACTTAAGCAA CAAGATCAAT GAGATGAAAA CCTTCAACAG CCCCAATCTG 
AAAGATGGGA GGTTTGTCAA TCCTGCCGGC CAGCCCACCC CTTATGCCAC CACTCAGCTC 
ATCCAGTCCA ACATAAGCAA CAACATCAAC ACGTCTGCCG GTGATGTGGA TGAAAAACAC 
TGGAAACCCC CGTGCCAACA GAAACCGGAT GTTACCCCAA TGCAGTACAA CATCATGGAG 
CAAAACAAGA TCAACAAAGA TTACCGAGCT TCGGACACCA TTCCTTCCAC CATTCCCTAC 
AACCAGTCCC ATGATCAGAA TGCCGGAGGG TCATACAACA GCTCAGACCG GGGAAGTAGC 
ACATCTGGGA GCCAAGGTCA TAAGAAGAGT ACTCGTACCC CGAAAGTATC AAAGCAGGCT 
GGCATGAACT GGGCAGATCT TTTGCCACCA CCACCTGCTC ACCCCCCACC ACATGGTGCC 
ACTGAAGACT ACACTTTGTC AGCAGATGAA AGCTATGACA GAGAGTTACC TTGCCCAATA 
CCACCTACAA GAATGTATCT TCAGCAAGAC GAACTGGAAG AGGAAGATGA AAGGGGTCCC 
ACACCTCCTA TTAGAGGAGC AGCTTCTTCT CCGGCTGCTG TGTCCTACAG CCATCAGTCC 
ACTGCCACTC TTACCCCATC ACCTCAGGAC GAACTCCAGC CAATGCTTCA GGACTGTCCC 
GAGGAGATCG GTCATACTCA GCATCAGCTA GAAAGAAGGC GTCATCAAAT TAGTCCTCCT 
CCACCTCCAA GGCCCATTTC CCCGCCTCAT ACATATGGTT ATATTTCTGG ACCAATAGTC 
TCCGATATGG ATACTGATGT TCCTGAAGAG GAAGGGGATG ATGGGGAAAT TGAAGTCACC 
AAGATGCAAA GTCGCAGACT TCTTTTACGT GGCCTGGAGC AGACTCCTGC TTCCAGTGTT 
GGAGACCTAG AAAGTTCTGT GACTGGTTCC ATGATCAATG GATGGGGCTC AGCTTCTGAA 
GAAGACAATG TTTCCAGTGG ACGTTCCAGT GTCAGTTCAT CGGATGGATC ATTTTTCACG 
GATGCGGATT TTGCACAGGC TGTCGCCGCA GCCGCAGAAT ATGCTGGGCT TAAAGTAGCA 
AGGCATCAAA TGCAGGAAGC AGCAGGAGTG TCGCGTCTTT GCCTCCCAGG TCGAAGACCT 
TTCCACGCGT CTCACTGTCC AAGACCAACA AGCCCAGTGT CAACAGACAG CAACATGAGC 
ACCGCTGTCA TACAGAAAAG CCGTCCTGCC AAAAAACAAA AACACCAGCA GGGACATCTT 
CGGAAAGATG TCTACACAGA TGATTTGCCA CCACCGCCAC TGCCACCTCC TGCTATCAAA 
TCTCCAACTG GTCCATCCAA AGCACAACTA GAAGGACGGC CTGGCCTTTC ACAGAAGTAC 
CTTGTTGCAG AACAAAGACT GGAAAGGCCA GGAGAGAGGA AAGGTGTCAG CTACAGGGGC 
AGGGAAGGTC CTGACAGCAG ACAACAACAT GCAGAAGCAA GAACCAATTC AGGAGAGAGA 
AGAGAGTCTT CGGAACAGCA ACTTGATGGA AAGATGAGAA AAGGGAAAGC TTCAAAGTGG 
GATGGGGCAC CTATAGTAGC AAATCAAATA CAAGAGGATA TATTACCCTA TTGCAAACCA 
ACATTCCCCA CTTCAAACAA CCCTCGTGAT CCCAGTTCCT CCAGCTCAAT GTCCTCAAGA 
GGATCTGGTG GACGACGGAG GGGAGAACAA ACAACAGGTC GCAGAAATGC AGCAGAGATG 
CAGCTCTTAG GAAATTATGA ACGAGGGGTT GATGAAGATG AAATAGAGGA AACAGAAAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811710.3
CDS	16..5058
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISETANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956256.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956256.3

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTGGCG TACCAGCGAG GAGGGGAAGC TGTAAGCAGC 
GCAGGCCGGC CTGGGCTCTT AAATATTTCT GAGACTGCTA ATCAGCCGTG GCTCGCAGAT 
ACCTGGCCAA ACACTGGAAA CAATCACAAC GAATGCCCCA TCAACTGCTG CACCTCCGGG 
AATGGCAACA GCGACAGCAA CCTCGCCACT TACAGCCGCC CAGCTGACTG CATAGCGAAT 
TACAACAACC AGTTGGACAG CAAGCAGACA AACCTGATGG TGCCCGACTC GGCTGTTTAT 
GGGGATGTAG ACTTAAGCAA CAAGATCAAT GAGATGAAAA CCTTCAACAG CCCCAATCTG 
AAAGATGGGA GGTTTGTCAA TCCTGCCGGC CAGCCCACCC CTTATGCCAC CACTCAGCTC 
ATCCAGTCCA ACATAAGCAA CAACATCAAC ACGTCTGCCG GTGATGTGGA TGAAAAACAC 
TGGAAACCCC CGTGCCAACA GAAACCGGAT GTTACCCCAA TGCAGTACAA CATCATGGAG 
CAAAACAAGA TCAACAAAGA TTACCGAGCT TCGGACACCA TTCCTTCCAC CATTCCCTAC 
AACCAGTCCC ATGATCAGAA TGCCGGAGGG TCATACAACA GCTCAGACCG GGGAAGTAGC 
ACATCTGGGA GCCAAGGTCA TAAGAAGAGT ACTCGTACCC CGAAAGTATC AAAGCAGGCT 
GGCATGAACT GGGCAGATCT TTTGCCACCA CCACCTGCTC ACCCCCCACC ACATGGTGCC 
ACTGAAGACT ACACTTTGTC AGCAGATGAA AGCTATGACA GAGAGTTACC TTGCCCAATA 
CCACCTACAA GAATGTATCT TCAGCAAGAC GAACTGGAAG AGGAAGATGA AAGGGGTCCC 
ACACCTCCTA TTAGAGGAGC AGCTTCTTCT CCGGCTGCTG TGTCCTACAG CCATCAGTCC 
ACTGCCACTC TTACCCCATC ACCTCAGGAC GAACTCCAGC CAATGCTTCA GGACTGTCCC 
GAGGAGATCG GTCATACTCA GCATCAGCTA GAAAGAAGGC GTCATCAAAT TAGTCCTCCT 
CCACCTCCAA GGCCCATTTC CCCGCCTCAT ACATATGGTT ATATTTCTGG ACCAATAGTC 
TCCGATATGG ATACTGATGT TCCTGAAGAG GAAGGGGATG ATGGGGAAAT TGAAGTCACC 
AAGATGCAAA GTCGCAGACT TCTTTTACGT GGCCTGGAGC AGACTCCTGC TTCCAGTGTT 
GGAGACCTAG AAAGTTCTGT GACTGGTTCC ATGATCAATG GATGGGGCTC AGCTTCTGAA 
GAAGACAATG TTTCCAGTGG ACGTTCCAGT GTCAGTTCAT CGGATGGATC ATTTTTCACG 
GATGCGGATT TTGCACAGGC TGTCGCCGCA GCCGCAGAAT ATGCTGGGCT TAAAGTAGCA 
AGGCATCAAA TGCAGGAAGC AGCAGGAGTG TCGCGTCTTT GCCTCCCAGG TCGAAGACCT 
TTCCACGCGT CTCACTGTCC AAGACCAACA AGCCCAGTGT CAACAGACAG CAACATGAGC 
ACCGCTGTCA TACAGAAAAG CCGTCCTGCC AAAAAACAAA AACACCAGCA GGGACATCTT 
CGGAAAGATG TCTACACAGA TGATTTGCCA CCACCGCCAC TGCCACCTCC TGCTATCAAA 
TCTCCAACTG GTCCATCCAA AGCACAACTA GAAGGACGGC CTGGCCTTTC ACAGAAGTAC 
CTTGTTGCAG AACAAAGACT GGAAAGGCCA GGAGAGAGGA AAGGTGTCAG CTACAGGGGC 
AGGGAAGGTC CTGACAGCAG ACAACAACAT GCAGAAGCAA GAACCAATTC AGGAGAGAGA 
AGAGAGTCTT CGGAACAGCA ACTTGATGGA AAGATGAGAA AAGGGAAAGC TTCAAAGTGG 
GATGGGGCAC CTATAGTAGC AAATCAAATA CAAGAGGATA TATTACCCTA TTGCAAACCA 
ACATTCCCCA CTTCAAACAA CCCTCGTGAT CCCAGTTCCT CCAGCTCAAT GTCCTCAAGA 
GGATCTGGTG GACGACGGAG GGGAGAACAA ACAACAGGTC GCAGAAATGC AGCAGAGATG 
CAGCTCTTAG GAAATTATGA ACGAGGGGTT GATGAAGATG AAATAGAGGA AACAGAAAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956258.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811712.3 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X2, mRNA.

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CloneID	OXa51063
Clone ID Related Accession (Same CDS sequence)	XM_012956258.3
Accession Version	XM_012956258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGAGCCTCCG AATTTTGTTG TCAAACCTCG TGACCAAGTT 
GTTGCTCTGG GACGCACAGT CACATTTCAG TGTGAAGCCA CCGGAAACCC CCAGCCAGCC 
ATTTTTTGGC AAAAGGAGGG AAGCCAGAAC CTTCTCTTTT CCTACCAACC TCCACAATCG 
TCGAGCCGCT TCTCCGTGTC CCAGACTGGT GACCTGACTA TAACCACCGT TCAGAAGTCA 
GACGTGGGCT ACTACATCTG CCAGACTCTC AACGTTGCCG GGAGCATTAC AACAAAAGCC 
TTCTTAGAAG TTACCGATGT GGTGGCCGAC CGTCCTCCTC CAGTCATTCG TCAGGGACCC 
GTGAATCAGA CAGTAGCTGT TGATGGCACC TTAATAATGA ACTGTATCGC CAGCGGCATT 
CCTGTTCCCA CAATCCACTG GAGAAAGGAC GGGATCCTGA TACCGACCCA AGACTCTCGC 
ATTAAACAGC AGGACTCAGG AGCTCTGCAG ATCCGTTACG CCAAGCTGAG TGATACCGGT 
CGGTACACCT GCACTGCCTC CACTACTAGC GGGGAAGCAA ACTGGAGCGC GTACATAGAA 
GTCCAAGAAT TTGGAGTTCC GGTTCAACCA CCCAGAGCCA CTGATCCCAA CCTGATTCCA 
AGTGCTCCAT CAAAACCAGA AGTCACGGAC GTCAGCAAAA ATACTGTAAC TTTATCCTGG 
CAGCCAAACC TCAACTCAGG TGCTACTCCT ACGTCGTACC TCATAGAGGC CTTCAGCCAT 
GCGTCTGGAA GCAGCTGGCA AACAGTGGCA GAAAATGTCA AGACCGATTC CTTTGCAGTT 
AAAGCACTGA AGCCCAATGC AATCTACCTC TTCCTCGTGA GAGCAGTAAA CGCATACGGA 
TTAAGTGATC CAAGCCAAAT ATCCGATCCT GTAAAAACAC AAGATATTCC TCCTACAAGT 
CAAGGAGTGG ATCACAAACA GGTGCAGAGA GAACTGGGCG ATGTTGTGCT ACACCTTCAT 
AACCCAACAA TACTTTCCTC TTCCTCCATC GAAGTACATT GGACTGTGGA CCAGCAATCA 
CAGTACATCC AAGGATACAA GATCCTCTAC AGGCCAAGCG CAGAGAATCG AGGCGAATCT 
GCATGGTCCA TATTTGAAGT CAGGACTCCG GCCAAAAACA GCGCAGTTAT TCCTGAACTG 
AGAAAAGGCG TTAACTACGA GATCAAAGCC CGCCCCTTTT TTAATGAGTT TCAAGGAGCT 
GATAGTGAAG TGCGGTTCGC TAAGACAATG GAAGAAGCAC CCAGTGCTCC CCCGCAGAGC 
GTTACTGTAA CCAAGAATGA TGGGAATGGA ACTGCTATTG TTGTGGCATG GCAGCCTCCC 
CCTGAAGATA CTCAGAATGG GATGGTCCAG GAATACAAGG TTTGGTGCCT GGGGAATGAG 
AGTCGCTATC ACATAAACAA GACTGTCGAT GGCTCTACCC TTTCCGTGGT CATCCCGTCC 
TTAGTCCCTG GTATCAAATA CAGCGTGGAG GTGGCAGCCA GCACGGGCGC AGGATCCGGT 
GTGAAAAGTG AACCCAACTA CATCCAGTTA GATTCCTTTG GAAACCCGGT GCCTCCGGGG 
GACCAAGTCA GCCTGGCCCA GCAGATATCC GATGTGGTGA AGCAGCCGGC ATTCATAGCA 
GGGATTGGTG CTGCCTGCTG GATAATCCTC ATGGTATTCA GTATCTGGTT GTACCGGCAC 
AGAAAGAAAC GCAATGGGCT CACCAGTTCC TATGCTGGAA TACGGAAAGT CCCGTCTTTT 
ACCTTCACAC CAACAGTGGC GTACCAGCGA GGAGGGGAAG CTGTAAGCAG CGCAGGCCGG 
CCTGGGCTCT TAAATATTTC TGAGACTGCT AATCAGCCGT GGCTCGCAGA TACCTGGCCA 
AACACTGGAA ACAATCACAA CGAATGCCCC ATCAACTGCT GCACCTCCGG GAATGGCAAC 
AGCGACAGCA ACCTCGCCAC TTACAGCCGC CCAGCTGACT GCATAGCGAA TTACAACAAC 
CAGTTGGACA GCAAGCAGAC AAACCTGATG GTGCCCGACT CGGCTGTTTA TGGGGATGTA 
GACTTAAGCA ACAAGATCAA TGAGATGAAA ACCTTCAACA GCCCCAATCT GAAAGATGGG 
AGGTTTGTCA ATCCTGCCGG CCAGCCCACC CCTTATGCCA CCACTCAGCT CATCCAGTCC 
AACATAAGCA ACAACATCAA CACGTCTGCC GGTGATGTGG ATGAAAAACA CTGGAAACCC 
CCGTGCCAAC AGAAACCGGA TGTTACCCCA ATGCAGTACA ACATCATGGA GCAAAACAAG 
ATCAACAAAG ATTACCGAGC TTCGGACACC ATTCCTTCCA CCATTCCCTA CAACCAGTCC 
CATGATCAGA ATGCCGGAGG GTCATACAAC AGCTCAGACC GGGGAAGTAG CACATCTGGG 
AGCCAAGGTC ATAAGAAGAG TACTCGTACC CCGAAAGTAT CAAAGCAGGC TGGCATGAAC 
TGGGCAGATC TTTTGCCACC ACCACCTGCT CACCCCCCAC CACATGGTGC CACTGAAGAC 
TACACTTTGT CAGCAGATGA AAGCTATGAC AGAGAGTTAC CTTGCCCAAT ACCACCTACA 
AGAATGTATC TTCAGCAAGA CGAACTGGAA GAGGAAGATG AAAGGGGTCC CACACCTCCT 
ATTAGAGGAG CAGCTTCTTC TCCGGCTGCT GTGTCCTACA GCCATCAGTC CACTGCCACT 
CTTACCCCAT CACCTCAGGA CGAACTCCAG CCAATGCTTC AGGACTGTCC CGAGGAGATC 
GGTCATACTC AGCATCAGCT AGAAAGAAGG CGTCATCAAA TTAGTCCTCC TCCACCTCCA 
AGGCCCATTT CCCCGCCTCA TACATATGGT TATATTTCTG GACCAATAGT CTCCGATATG 
GATACTGATG TTCCTGAAGA GGAAGGGGAT GATGGGGAAA TTGAAGTCAC CAAGATGCAA 
AGTCGCAGAC TTCTTTTACG TGGCCTGGAG CAGACTCCTG CTTCCAGTGT TGGAGACCTA 
GAAAGTTCTG TGACTGGTTC CATGATCAAT GGATGGGGCT CAGCTTCTGA AGAAGACAAT 
GTTTCCAGTG GACGTTCCAG TGTCAGTTCA TCGGATGGAT CATTTTTCAC GGATGCGGAT 
TTTGCACAGG CTGTCGCCGC AGCCGCAGAA TATGCTGGGC TTAAAGTAGC AAGGCATCAA 
ATGCAGGAAG CAGCAGGAGT GTCGCGTCTT TGCCTCCCAG GTCGAAGACC TTTCCACGCG 
TCTCACTGTC CAAGACCAAC AAGCCCAGTG TCAACAGACA GCAACATGAG CACCGCTGTC 
ATACAGAAAA GCCGTCCTGC CAAAAAACAA AAACACCAGC AGGGACATCT TCGGAAAGAT 
GTCTACACAG ATGATTTGCC ACCACCGCCA CTGCCACCTC CTGCTATCAA ATCTCCAACT 
GGTCCATCCA AAGCACAACT AGAAGGACGG CCTGGCCTTT CACAGAAGTA CCTTGTTGCA 
GAACAAAGAC TGGAAAGGCC AGGAGAGAGG AAAGGTGTCA GCTACAGGGG CAGGGAAGGT 
CCTGACAGCA GACAACAACA TGCAGAAGCA AGAACCAATT CAGGAGAGAG AAGAGAGTCT 
TCGGAACAGC AACTTGATGG AAAGATGAGA AAAGGGAAAG CTTCAAAGTG GGATGGGGCA 
CCTATAGTAG CAAATCAAAT ACAAGAGGAT ATATTACCCT ATTGCAAACC AACATTCCCC 
ACTTCAAACA ACCCTCGTGA TCCCAGTTCC TCCAGCTCAA TGTCCTCAAG AGGATCTGGT 
GGACGACGGA GGGGAGAACA AACAACAGGT CGCAGAAATG CAGCAGAGAT GCAGCTCTTA 
GGAAATTATG AACGAGGGGT TGATGAAGAT GAAATAGAGG AAACAGAAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811712.3
CDS	16..5049
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISETANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956258.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956258.3

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGAGCCTCCG AATTTTGTTG TCAAACCTCG TGACCAAGTT 
GTTGCTCTGG GACGCACAGT CACATTTCAG TGTGAAGCCA CCGGAAACCC CCAGCCAGCC 
ATTTTTTGGC AAAAGGAGGG AAGCCAGAAC CTTCTCTTTT CCTACCAACC TCCACAATCG 
TCGAGCCGCT TCTCCGTGTC CCAGACTGGT GACCTGACTA TAACCACCGT TCAGAAGTCA 
GACGTGGGCT ACTACATCTG CCAGACTCTC AACGTTGCCG GGAGCATTAC AACAAAAGCC 
TTCTTAGAAG TTACCGATGT GGTGGCCGAC CGTCCTCCTC CAGTCATTCG TCAGGGACCC 
GTGAATCAGA CAGTAGCTGT TGATGGCACC TTAATAATGA ACTGTATCGC CAGCGGCATT 
CCTGTTCCCA CAATCCACTG GAGAAAGGAC GGGATCCTGA TACCGACCCA AGACTCTCGC 
ATTAAACAGC AGGACTCAGG AGCTCTGCAG ATCCGTTACG CCAAGCTGAG TGATACCGGT 
CGGTACACCT GCACTGCCTC CACTACTAGC GGGGAAGCAA ACTGGAGCGC GTACATAGAA 
GTCCAAGAAT TTGGAGTTCC GGTTCAACCA CCCAGAGCCA CTGATCCCAA CCTGATTCCA 
AGTGCTCCAT CAAAACCAGA AGTCACGGAC GTCAGCAAAA ATACTGTAAC TTTATCCTGG 
CAGCCAAACC TCAACTCAGG TGCTACTCCT ACGTCGTACC TCATAGAGGC CTTCAGCCAT 
GCGTCTGGAA GCAGCTGGCA AACAGTGGCA GAAAATGTCA AGACCGATTC CTTTGCAGTT 
AAAGCACTGA AGCCCAATGC AATCTACCTC TTCCTCGTGA GAGCAGTAAA CGCATACGGA 
TTAAGTGATC CAAGCCAAAT ATCCGATCCT GTAAAAACAC AAGATATTCC TCCTACAAGT 
CAAGGAGTGG ATCACAAACA GGTGCAGAGA GAACTGGGCG ATGTTGTGCT ACACCTTCAT 
AACCCAACAA TACTTTCCTC TTCCTCCATC GAAGTACATT GGACTGTGGA CCAGCAATCA 
CAGTACATCC AAGGATACAA GATCCTCTAC AGGCCAAGCG CAGAGAATCG AGGCGAATCT 
GCATGGTCCA TATTTGAAGT CAGGACTCCG GCCAAAAACA GCGCAGTTAT TCCTGAACTG 
AGAAAAGGCG TTAACTACGA GATCAAAGCC CGCCCCTTTT TTAATGAGTT TCAAGGAGCT 
GATAGTGAAG TGCGGTTCGC TAAGACAATG GAAGAAGCAC CCAGTGCTCC CCCGCAGAGC 
GTTACTGTAA CCAAGAATGA TGGGAATGGA ACTGCTATTG TTGTGGCATG GCAGCCTCCC 
CCTGAAGATA CTCAGAATGG GATGGTCCAG GAATACAAGG TTTGGTGCCT GGGGAATGAG 
AGTCGCTATC ACATAAACAA GACTGTCGAT GGCTCTACCC TTTCCGTGGT CATCCCGTCC 
TTAGTCCCTG GTATCAAATA CAGCGTGGAG GTGGCAGCCA GCACGGGCGC AGGATCCGGT 
GTGAAAAGTG AACCCAACTA CATCCAGTTA GATTCCTTTG GAAACCCGGT GCCTCCGGGG 
GACCAAGTCA GCCTGGCCCA GCAGATATCC GATGTGGTGA AGCAGCCGGC ATTCATAGCA 
GGGATTGGTG CTGCCTGCTG GATAATCCTC ATGGTATTCA GTATCTGGTT GTACCGGCAC 
AGAAAGAAAC GCAATGGGCT CACCAGTTCC TATGCTGGAA TACGGAAAGT CCCGTCTTTT 
ACCTTCACAC CAACAGTGGC GTACCAGCGA GGAGGGGAAG CTGTAAGCAG CGCAGGCCGG 
CCTGGGCTCT TAAATATTTC TGAGACTGCT AATCAGCCGT GGCTCGCAGA TACCTGGCCA 
AACACTGGAA ACAATCACAA CGAATGCCCC ATCAACTGCT GCACCTCCGG GAATGGCAAC 
AGCGACAGCA ACCTCGCCAC TTACAGCCGC CCAGCTGACT GCATAGCGAA TTACAACAAC 
CAGTTGGACA GCAAGCAGAC AAACCTGATG GTGCCCGACT CGGCTGTTTA TGGGGATGTA 
GACTTAAGCA ACAAGATCAA TGAGATGAAA ACCTTCAACA GCCCCAATCT GAAAGATGGG 
AGGTTTGTCA ATCCTGCCGG CCAGCCCACC CCTTATGCCA CCACTCAGCT CATCCAGTCC 
AACATAAGCA ACAACATCAA CACGTCTGCC GGTGATGTGG ATGAAAAACA CTGGAAACCC 
CCGTGCCAAC AGAAACCGGA TGTTACCCCA ATGCAGTACA ACATCATGGA GCAAAACAAG 
ATCAACAAAG ATTACCGAGC TTCGGACACC ATTCCTTCCA CCATTCCCTA CAACCAGTCC 
CATGATCAGA ATGCCGGAGG GTCATACAAC AGCTCAGACC GGGGAAGTAG CACATCTGGG 
AGCCAAGGTC ATAAGAAGAG TACTCGTACC CCGAAAGTAT CAAAGCAGGC TGGCATGAAC 
TGGGCAGATC TTTTGCCACC ACCACCTGCT CACCCCCCAC CACATGGTGC CACTGAAGAC 
TACACTTTGT CAGCAGATGA AAGCTATGAC AGAGAGTTAC CTTGCCCAAT ACCACCTACA 
AGAATGTATC TTCAGCAAGA CGAACTGGAA GAGGAAGATG AAAGGGGTCC CACACCTCCT 
ATTAGAGGAG CAGCTTCTTC TCCGGCTGCT GTGTCCTACA GCCATCAGTC CACTGCCACT 
CTTACCCCAT CACCTCAGGA CGAACTCCAG CCAATGCTTC AGGACTGTCC CGAGGAGATC 
GGTCATACTC AGCATCAGCT AGAAAGAAGG CGTCATCAAA TTAGTCCTCC TCCACCTCCA 
AGGCCCATTT CCCCGCCTCA TACATATGGT TATATTTCTG GACCAATAGT CTCCGATATG 
GATACTGATG TTCCTGAAGA GGAAGGGGAT GATGGGGAAA TTGAAGTCAC CAAGATGCAA 
AGTCGCAGAC TTCTTTTACG TGGCCTGGAG CAGACTCCTG CTTCCAGTGT TGGAGACCTA 
GAAAGTTCTG TGACTGGTTC CATGATCAAT GGATGGGGCT CAGCTTCTGA AGAAGACAAT 
GTTTCCAGTG GACGTTCCAG TGTCAGTTCA TCGGATGGAT CATTTTTCAC GGATGCGGAT 
TTTGCACAGG CTGTCGCCGC AGCCGCAGAA TATGCTGGGC TTAAAGTAGC AAGGCATCAA 
ATGCAGGAAG CAGCAGGAGT GTCGCGTCTT TGCCTCCCAG GTCGAAGACC TTTCCACGCG 
TCTCACTGTC CAAGACCAAC AAGCCCAGTG TCAACAGACA GCAACATGAG CACCGCTGTC 
ATACAGAAAA GCCGTCCTGC CAAAAAACAA AAACACCAGC AGGGACATCT TCGGAAAGAT 
GTCTACACAG ATGATTTGCC ACCACCGCCA CTGCCACCTC CTGCTATCAA ATCTCCAACT 
GGTCCATCCA AAGCACAACT AGAAGGACGG CCTGGCCTTT CACAGAAGTA CCTTGTTGCA 
GAACAAAGAC TGGAAAGGCC AGGAGAGAGG AAAGGTGTCA GCTACAGGGG CAGGGAAGGT 
CCTGACAGCA GACAACAACA TGCAGAAGCA AGAACCAATT CAGGAGAGAG AAGAGAGTCT 
TCGGAACAGC AACTTGATGG AAAGATGAGA AAAGGGAAAG CTTCAAAGTG GGATGGGGCA 
CCTATAGTAG CAAATCAAAT ACAAGAGGAT ATATTACCCT ATTGCAAACC AACATTCCCC 
ACTTCAAACA ACCCTCGTGA TCCCAGTTCC TCCAGCTCAA TGTCCTCAAG AGGATCTGGT 
GGACGACGGA GGGGAGAACA AACAACAGGT CGCAGAAATG CAGCAGAGAT GCAGCTCTTA 
GGAAATTATG AACGAGGGGT TGATGAAGAT GAAATAGAGG AAACAGAAAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956259.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811713.3 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa51064
Clone ID Related Accession (Same CDS sequence)	XM_012956259.3
Accession Version	XM_012956259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956259.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTGGCG TACCAGCGAG GAGGGGAAGC TGTAAGCAGC 
GCAGGCCGGC CTGGGCTCTT AAATATTTCT GAGACTGCTA ATCAGCCGTG GCTCGCAGAT 
ACCTGGCCAA ACACTGGAAA CAATCACAAC GAATGCCCCA TCAACTGCTG CACCTCCGGG 
AATGGCAACA GCGACAGCAA CCTCGCCACT TACAGCCGCC CAGCTGACTG CATAGCGAAT 
TACAACAACC AGTTGGACAG CAAGCAGACA AACCTGATGG TGCCCGACTC GGCTGTTTAT 
GGGGATGTAG ACTTAAGCAA CAAGATCAAT GAGATGAAAA CCTTCAACAG CCCCAATCTG 
AAAGATGGGA GGTTTGTCAA TCCTGCCGGC CAGCCCACCC CTTATGCCAC CACTCAGCTC 
ATCCAGTCCA ACATAAGCAA CAACATCAAC ACGTCTGCCG GTGATGTGGA TGAAAAACAC 
TGGAAACCCC CGTGCCAACA GAAACCGGAT GTTACCCCAA TGCAGTACAA CATCATGGAG 
CAAAACAAGA TCAACAAAGA TTACCGAGCT TCGGACACCA TTCCTTCCAC CATTCCCTAC 
AACCAGTCCC ATGATCAGAA TGCCGGAGGG TCATACAACA GCTCAGACCG GGGAAGTAGC 
ACATCTGGGA GCCAAGGTCA TAAGAAGAGT ACTCGTACCC CGAAAGTATC AAAGCAGGCT 
GGCATGAACT GGGCAGATCT TTTGCCACCA CCACCTGCTC ACCCCCCACC ACATGGTGCC 
ACTGAAGACT ACACTTTGTC AGCAGATGAA AGCTATGACA GAGAGTTACC TTGCCCAATA 
CCACCTACAA GAATGTATCT TCAGCAAGAC GAACTGGAAG AGGAAGATGA AAGGGGTCCC 
ACACCTCCTA TTAGAGGAGC AGCTTCTTCT CCGGCTGCTG TGTCCTACAG CCATCAGTCC 
ACTGCCACTC TTACCCCATC ACCTCAGGAC GAACTCCAGC CAATGCTTCA GGACTGTCCC 
GAGGAGATCG GTCATACTCA GCATCAGCTA GAAAGAAGGC GTCATCAAAT TAGTCCTCCT 
CCACCTCCAA GGCCCATTTC CCCGCCTCAT ACATATGGTT ATATTTCTGG ACCAATAGTC 
TCCGATATGG ATACTGATGT TCCTGAAGAG GAAGGGGATG ATGGGGAAAT TGAAGTCACC 
AAGATGCAAA GTCGCAGACT TCTTTTACGT GGCCTGGAGC AGACTCCTGC TTCCAGTGTT 
GGAGACCTAG AAAGTTCTGT GACTGGTTCC ATGATCAATG GATGGGGCTC AGCTTCTGAA 
GAAGACAATG TTTCCAGTGG ACGTTCCAGT GTCAGTTCAT CGGATGGATC ATTTTTCACG 
GATGCGGATT TTGCACAGGC TGTCGCCGCA GCCGCAGAAT ATGCTGGGCT TAAAGTAGCA 
AGGCATCAAA TGCAGGAAGC AGCAGGAGGT CGAAGACCTT TCCACGCGTC TCACTGTCCA 
AGACCAACAA GCCCAGTGTC AACAGACAGC AACATGAGCA CCGCTGTCAT ACAGAAAAGC 
CGTCCTGCCA AAAAACAAAA ACACCAGCAG GGACATCTTC GGAAAGATGT CTACACAGAT 
GATTTGCCAC CACCGCCACT GCCACCTCCT GCTATCAAAT CTCCAACTGG TCCATCCAAA 
GCACAACTAG AAGGACGGCC TGGCCTTTCA CAGAAGTACC TTGTTGCAGA ACAAAGACTG 
GAAAGGCCAG GAGAGAGGAA AGGTGTCAGC TACAGGGGCA GGGAAGGTCC TGACAGCAGA 
CAACAACATG CAGAAGCAAG AACCAATTCA GGAGAGAGAA GAGAGTCTTC GGAACAGCAA 
CTTGATGGAA AGATGAGAAA AGGGAAAGCT TCAAAGTGGG ATGGGGCACC TATAGTAGCA 
AATCAAATAC AAGAGGATAT ATTACCCTAT TGCAAACCAA CATTCCCCAC TTCAAACAAC 
CCTCGTGATC CCAGTTCCTC CAGCTCAATG TCCTCAAGAG GATCTGGTGG ACGACGGAGG 
GGAGAACAAA CAACAGGTCG CAGAAATGCA GCAGAGATGC AGCTCTTAGG AAATTATGAA 
CGAGGGGTTG ATGAAGATGA AATAGAGGAA ACAGAAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811713.3
CDS	16..5037
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISETANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956259.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956259.3

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTC 
CCGTCTTTTA CCTTCACACC AACAGTGGCG TACCAGCGAG GAGGGGAAGC TGTAAGCAGC 
GCAGGCCGGC CTGGGCTCTT AAATATTTCT GAGACTGCTA ATCAGCCGTG GCTCGCAGAT 
ACCTGGCCAA ACACTGGAAA CAATCACAAC GAATGCCCCA TCAACTGCTG CACCTCCGGG 
AATGGCAACA GCGACAGCAA CCTCGCCACT TACAGCCGCC CAGCTGACTG CATAGCGAAT 
TACAACAACC AGTTGGACAG CAAGCAGACA AACCTGATGG TGCCCGACTC GGCTGTTTAT 
GGGGATGTAG ACTTAAGCAA CAAGATCAAT GAGATGAAAA CCTTCAACAG CCCCAATCTG 
AAAGATGGGA GGTTTGTCAA TCCTGCCGGC CAGCCCACCC CTTATGCCAC CACTCAGCTC 
ATCCAGTCCA ACATAAGCAA CAACATCAAC ACGTCTGCCG GTGATGTGGA TGAAAAACAC 
TGGAAACCCC CGTGCCAACA GAAACCGGAT GTTACCCCAA TGCAGTACAA CATCATGGAG 
CAAAACAAGA TCAACAAAGA TTACCGAGCT TCGGACACCA TTCCTTCCAC CATTCCCTAC 
AACCAGTCCC ATGATCAGAA TGCCGGAGGG TCATACAACA GCTCAGACCG GGGAAGTAGC 
ACATCTGGGA GCCAAGGTCA TAAGAAGAGT ACTCGTACCC CGAAAGTATC AAAGCAGGCT 
GGCATGAACT GGGCAGATCT TTTGCCACCA CCACCTGCTC ACCCCCCACC ACATGGTGCC 
ACTGAAGACT ACACTTTGTC AGCAGATGAA AGCTATGACA GAGAGTTACC TTGCCCAATA 
CCACCTACAA GAATGTATCT TCAGCAAGAC GAACTGGAAG AGGAAGATGA AAGGGGTCCC 
ACACCTCCTA TTAGAGGAGC AGCTTCTTCT CCGGCTGCTG TGTCCTACAG CCATCAGTCC 
ACTGCCACTC TTACCCCATC ACCTCAGGAC GAACTCCAGC CAATGCTTCA GGACTGTCCC 
GAGGAGATCG GTCATACTCA GCATCAGCTA GAAAGAAGGC GTCATCAAAT TAGTCCTCCT 
CCACCTCCAA GGCCCATTTC CCCGCCTCAT ACATATGGTT ATATTTCTGG ACCAATAGTC 
TCCGATATGG ATACTGATGT TCCTGAAGAG GAAGGGGATG ATGGGGAAAT TGAAGTCACC 
AAGATGCAAA GTCGCAGACT TCTTTTACGT GGCCTGGAGC AGACTCCTGC TTCCAGTGTT 
GGAGACCTAG AAAGTTCTGT GACTGGTTCC ATGATCAATG GATGGGGCTC AGCTTCTGAA 
GAAGACAATG TTTCCAGTGG ACGTTCCAGT GTCAGTTCAT CGGATGGATC ATTTTTCACG 
GATGCGGATT TTGCACAGGC TGTCGCCGCA GCCGCAGAAT ATGCTGGGCT TAAAGTAGCA 
AGGCATCAAA TGCAGGAAGC AGCAGGAGGT CGAAGACCTT TCCACGCGTC TCACTGTCCA 
AGACCAACAA GCCCAGTGTC AACAGACAGC AACATGAGCA CCGCTGTCAT ACAGAAAAGC 
CGTCCTGCCA AAAAACAAAA ACACCAGCAG GGACATCTTC GGAAAGATGT CTACACAGAT 
GATTTGCCAC CACCGCCACT GCCACCTCCT GCTATCAAAT CTCCAACTGG TCCATCCAAA 
GCACAACTAG AAGGACGGCC TGGCCTTTCA CAGAAGTACC TTGTTGCAGA ACAAAGACTG 
GAAAGGCCAG GAGAGAGGAA AGGTGTCAGC TACAGGGGCA GGGAAGGTCC TGACAGCAGA 
CAACAACATG CAGAAGCAAG AACCAATTCA GGAGAGAGAA GAGAGTCTTC GGAACAGCAA 
CTTGATGGAA AGATGAGAAA AGGGAAAGCT TCAAAGTGGG ATGGGGCACC TATAGTAGCA 
AATCAAATAC AAGAGGATAT ATTACCCTAT TGCAAACCAA CATTCCCCAC TTCAAACAAC 
CCTCGTGATC CCAGTTCCTC CAGCTCAATG TCCTCAAGAG GATCTGGTGG ACGACGGAGG 
GGAGAACAAA CAACAGGTCG CAGAAATGCA GCAGAGATGC AGCTCTTAGG AAATTATGAA 
CGAGGGGTTG ATGAAGATGA AATAGAGGAA ACAGAAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956260.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811714.3 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X4, mRNA.

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CloneID	OXa51065
Clone ID Related Accession (Same CDS sequence)	XM_012956260.3
Accession Version	XM_012956260.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956260.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTG 
GCGTACCAGC GAGGAGGGGA AGCTGTAAGC AGCGCAGGCC GGCCTGGGCT CTTAAATATT 
TCTGAGACTG CTAATCAGCC GTGGCTCGCA GATACCTGGC CAAACACTGG AAACAATCAC 
AACGAATGCC CCATCAACTG CTGCACCTCC GGGAATGGCA ACAGCGACAG CAACCTCGCC 
ACTTACAGCC GCCCAGCTGA CTGCATAGCG AATTACAACA ACCAGTTGGA CAGCAAGCAG 
ACAAACCTGA TGGTGCCCGA CTCGGCTGTT TATGGGGATG TAGACTTAAG CAACAAGATC 
AATGAGATGA AAACCTTCAA CAGCCCCAAT CTGAAAGATG GGAGGTTTGT CAATCCTGCC 
GGCCAGCCCA CCCCTTATGC CACCACTCAG CTCATCCAGT CCAACATAAG CAACAACATC 
AACACGTCTG CCGGTGATGT GGATGAAAAA CACTGGAAAC CCCCGTGCCA ACAGAAACCG 
GATGTTACCC CAATGCAGTA CAACATCATG GAGCAAAACA AGATCAACAA AGATTACCGA 
GCTTCGGACA CCATTCCTTC CACCATTCCC TACAACCAGT CCCATGATCA GAATGCCGGA 
GGGTCATACA ACAGCTCAGA CCGGGGAAGT AGCACATCTG GGAGCCAAGG TCATAAGAAG 
AGTACTCGTA CCCCGAAAGT ATCAAAGCAG GCTGGCATGA ACTGGGCAGA TCTTTTGCCA 
CCACCACCTG CTCACCCCCC ACCACATGGT GCCACTGAAG ACTACACTTT GTCAGCAGAT 
GAAAGCTATG ACAGAGAGTT ACCTTGCCCA ATACCACCTA CAAGAATGTA TCTTCAGCAA 
GACGAACTGG AAGAGGAAGA TGAAAGGGGT CCCACACCTC CTATTAGAGG AGCAGCTTCT 
TCTCCGGCTG CTGTGTCCTA CAGCCATCAG TCCACTGCCA CTCTTACCCC ATCACCTCAG 
GACGAACTCC AGCCAATGCT TCAGGACTGT CCCGAGGAGA TCGGTCATAC TCAGCATCAG 
CTAGAAAGAA GGCGTCATCA AATTAGTCCT CCTCCACCTC CAAGGCCCAT TTCCCCGCCT 
CATACATATG GTTATATTTC TGGACCAATA GTCTCCGATA TGGATACTGA TGTTCCTGAA 
GAGGAAGGGG ATGATGGGGA AATTGAAGTC ACCAAGATGC AAAGTCGCAG ACTTCTTTTA 
CGTGGCCTGG AGCAGACTCC TGCTTCCAGT GTTGGAGACC TAGAAAGTTC TGTGACTGGT 
TCCATGATCA ATGGATGGGG CTCAGCTTCT GAAGAAGACA ATGTTTCCAG TGGACGTTCC 
AGTGTCAGTT CATCGGATGG ATCATTTTTC ACGGATGCGG ATTTTGCACA GGCTGTCGCC 
GCAGCCGCAG AATATGCTGG GCTTAAAGTA GCAAGGCATC AAATGCAGGA AGCAGCAGGA 
GTGTCGCGTC TTTGCCTCCC AGGTCGAAGA CCTTTCCACG CGTCTCACTG TCCAAGACCA 
ACAAGCCCAG TGTCAACAGA CAGCAACATG AGCACCGCTG TCATACAGAA AAGCCGTCCT 
GCCAAAAAAC AAAAACACCA GCAGGGACAT CTTCGGAAAG ATGTCTACAC AGATGATTTG 
CCACCACCGC CACTGCCACC TCCTGCTATC AAATCTCCAA CTGGTCCATC CAAAGCACAA 
CTAGAAGGAC GGCCTGGCCT TTCACAGAAG TACCTTGTTG CAGAACAAAG ACTGGAAAGG 
CCAGGAGAGA GGAAAGGTGT CAGCTACAGG GGCAGGGAAG GTCCTGACAG CAGACAACAA 
CATGCAGAAG CAAGAACCAA TTCAGGAGAG AGAAGAGAGT CTTCGGAACA GCAACTTGAT 
GGAAAGATGA GAAAAGGGAA AGCTTCAAAG TGGGATGGGG CACCTATAGT AGCAAATCAA 
ATACAAGAGG ATATATTACC CTATTGCAAA CCAACATTCC CCACTTCAAA CAACCCTCGT 
GATCCCAGTT CCTCCAGCTC AATGTCCTCA AGAGGATCTG GTGGACGACG GAGGGGAGAA 
CAAACAACAG GTCGCAGAAA TGCAGCAGAG ATGCAGCTCT TAGGAAATTA TGAACGAGGG 
GTTGATGAAG ATGAAATAGA GGAAACAGAA AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811714.3
CDS	16..5031
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVAYQRGGEAVSSAGRPGLLNISETANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956260.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956260.3

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTG 
GCGTACCAGC GAGGAGGGGA AGCTGTAAGC AGCGCAGGCC GGCCTGGGCT CTTAAATATT 
TCTGAGACTG CTAATCAGCC GTGGCTCGCA GATACCTGGC CAAACACTGG AAACAATCAC 
AACGAATGCC CCATCAACTG CTGCACCTCC GGGAATGGCA ACAGCGACAG CAACCTCGCC 
ACTTACAGCC GCCCAGCTGA CTGCATAGCG AATTACAACA ACCAGTTGGA CAGCAAGCAG 
ACAAACCTGA TGGTGCCCGA CTCGGCTGTT TATGGGGATG TAGACTTAAG CAACAAGATC 
AATGAGATGA AAACCTTCAA CAGCCCCAAT CTGAAAGATG GGAGGTTTGT CAATCCTGCC 
GGCCAGCCCA CCCCTTATGC CACCACTCAG CTCATCCAGT CCAACATAAG CAACAACATC 
AACACGTCTG CCGGTGATGT GGATGAAAAA CACTGGAAAC CCCCGTGCCA ACAGAAACCG 
GATGTTACCC CAATGCAGTA CAACATCATG GAGCAAAACA AGATCAACAA AGATTACCGA 
GCTTCGGACA CCATTCCTTC CACCATTCCC TACAACCAGT CCCATGATCA GAATGCCGGA 
GGGTCATACA ACAGCTCAGA CCGGGGAAGT AGCACATCTG GGAGCCAAGG TCATAAGAAG 
AGTACTCGTA CCCCGAAAGT ATCAAAGCAG GCTGGCATGA ACTGGGCAGA TCTTTTGCCA 
CCACCACCTG CTCACCCCCC ACCACATGGT GCCACTGAAG ACTACACTTT GTCAGCAGAT 
GAAAGCTATG ACAGAGAGTT ACCTTGCCCA ATACCACCTA CAAGAATGTA TCTTCAGCAA 
GACGAACTGG AAGAGGAAGA TGAAAGGGGT CCCACACCTC CTATTAGAGG AGCAGCTTCT 
TCTCCGGCTG CTGTGTCCTA CAGCCATCAG TCCACTGCCA CTCTTACCCC ATCACCTCAG 
GACGAACTCC AGCCAATGCT TCAGGACTGT CCCGAGGAGA TCGGTCATAC TCAGCATCAG 
CTAGAAAGAA GGCGTCATCA AATTAGTCCT CCTCCACCTC CAAGGCCCAT TTCCCCGCCT 
CATACATATG GTTATATTTC TGGACCAATA GTCTCCGATA TGGATACTGA TGTTCCTGAA 
GAGGAAGGGG ATGATGGGGA AATTGAAGTC ACCAAGATGC AAAGTCGCAG ACTTCTTTTA 
CGTGGCCTGG AGCAGACTCC TGCTTCCAGT GTTGGAGACC TAGAAAGTTC TGTGACTGGT 
TCCATGATCA ATGGATGGGG CTCAGCTTCT GAAGAAGACA ATGTTTCCAG TGGACGTTCC 
AGTGTCAGTT CATCGGATGG ATCATTTTTC ACGGATGCGG ATTTTGCACA GGCTGTCGCC 
GCAGCCGCAG AATATGCTGG GCTTAAAGTA GCAAGGCATC AAATGCAGGA AGCAGCAGGA 
GTGTCGCGTC TTTGCCTCCC AGGTCGAAGA CCTTTCCACG CGTCTCACTG TCCAAGACCA 
ACAAGCCCAG TGTCAACAGA CAGCAACATG AGCACCGCTG TCATACAGAA AAGCCGTCCT 
GCCAAAAAAC AAAAACACCA GCAGGGACAT CTTCGGAAAG ATGTCTACAC AGATGATTTG 
CCACCACCGC CACTGCCACC TCCTGCTATC AAATCTCCAA CTGGTCCATC CAAAGCACAA 
CTAGAAGGAC GGCCTGGCCT TTCACAGAAG TACCTTGTTG CAGAACAAAG ACTGGAAAGG 
CCAGGAGAGA GGAAAGGTGT CAGCTACAGG GGCAGGGAAG GTCCTGACAG CAGACAACAA 
CATGCAGAAG CAAGAACCAA TTCAGGAGAG AGAAGAGAGT CTTCGGAACA GCAACTTGAT 
GGAAAGATGA GAAAAGGGAA AGCTTCAAAG TGGGATGGGG CACCTATAGT AGCAAATCAA 
ATACAAGAGG ATATATTACC CTATTGCAAA CCAACATTCC CCACTTCAAA CAACCCTCGT 
GATCCCAGTT CCTCCAGCTC AATGTCCTCA AGAGGATCTG GTGGACGACG GAGGGGAGAA 
CAAACAACAG GTCGCAGAAA TGCAGCAGAG ATGCAGCTCT TAGGAAATTA TGAACGAGGG 
GTTGATGAAG ATGAAATAGA GGAAACAGAA AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956261.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811715.3 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X5, mRNA.

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CloneID	OXa51066
Clone ID Related Accession (Same CDS sequence)	XM_012956261.3
Accession Version	XM_012956261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGAGCCTCCG AATTTTGTTG TCAAACCTCG TGACCAAGTT 
GTTGCTCTGG GACGCACAGT CACATTTCAG TGTGAAGCCA CCGGAAACCC CCAGCCAGCC 
ATTTTTTGGC AAAAGGAGGG AAGCCAGAAC CTTCTCTTTT CCTACCAACC TCCACAATCG 
TCGAGCCGCT TCTCCGTGTC CCAGACTGGT GACCTGACTA TAACCACCGT TCAGAAGTCA 
GACGTGGGCT ACTACATCTG CCAGACTCTC AACGTTGCCG GGAGCATTAC AACAAAAGCC 
TTCTTAGAAG TTACCGATGT GGTGGCCGAC CGTCCTCCTC CAGTCATTCG TCAGGGACCC 
GTGAATCAGA CAGTAGCTGT TGATGGCACC TTAATAATGA ACTGTATCGC CAGCGGCATT 
CCTGTTCCCA CAATCCACTG GAGAAAGGAC GGGATCCTGA TACCGACCCA AGACTCTCGC 
ATTAAACAGC AGGACTCAGG AGCTCTGCAG ATCCGTTACG CCAAGCTGAG TGATACCGGT 
CGGTACACCT GCACTGCCTC CACTACTAGC GGGGAAGCAA ACTGGAGCGC GTACATAGAA 
GTCCAAGAAT TTGGAGTTCC GGTTCAACCA CCCAGAGCCA CTGATCCCAA CCTGATTCCA 
AGTGCTCCAT CAAAACCAGA AGTCACGGAC GTCAGCAAAA ATACTGTAAC TTTATCCTGG 
CAGCCAAACC TCAACTCAGG TGCTACTCCT ACGTCGTACC TCATAGAGGC CTTCAGCCAT 
GCGTCTGGAA GCAGCTGGCA AACAGTGGCA GAAAATGTCA AGACCGATTC CTTTGCAGTT 
AAAGCACTGA AGCCCAATGC AATCTACCTC TTCCTCGTGA GAGCAGTAAA CGCATACGGA 
TTAAGTGATC CAAGCCAAAT ATCCGATCCT GTAAAAACAC AAGATATTCC TCCTACAAGT 
CAAGGAGTGG ATCACAAACA GGTGCAGAGA GAACTGGGCG ATGTTGTGCT ACACCTTCAT 
AACCCAACAA TACTTTCCTC TTCCTCCATC GAAGTACATT GGACTGTGGA CCAGCAATCA 
CAGTACATCC AAGGATACAA GATCCTCTAC AGGCCAAGCG CAGAGAATCG AGGCGAATCT 
GCATGGTCCA TATTTGAAGT CAGGACTCCG GCCAAAAACA GCGCAGTTAT TCCTGAACTG 
AGAAAAGGCG TTAACTACGA GATCAAAGCC CGCCCCTTTT TTAATGAGTT TCAAGGAGCT 
GATAGTGAAG TGCGGTTCGC TAAGACAATG GAAGAAGCAC CCAGTGCTCC CCCGCAGAGC 
GTTACTGTAA CCAAGAATGA TGGGAATGGA ACTGCTATTG TTGTGGCATG GCAGCCTCCC 
CCTGAAGATA CTCAGAATGG GATGGTCCAG GAATACAAGG TTTGGTGCCT GGGGAATGAG 
AGTCGCTATC ACATAAACAA GACTGTCGAT GGCTCTACCC TTTCCGTGGT CATCCCGTCC 
TTAGTCCCTG GTATCAAATA CAGCGTGGAG GTGGCAGCCA GCACGGGCGC AGGATCCGGT 
GTGAAAAGTG AACCCAACTA CATCCAGTTA GATTCCTTTG GAAACCCGGT GCCTCCGGGG 
GACCAAGTCA GCCTGGCCCA GCAGATATCC GATGTGGTGA AGCAGCCGGC ATTCATAGCA 
GGGATTGGTG CTGCCTGCTG GATAATCCTC ATGGTATTCA GTATCTGGTT GTACCGGCAC 
AGAAAGAAAC GCAATGGGCT CACCAGTTCC TATGCTGGAA TACGGAAAGT CCCGTCTTTT 
ACCTTCACAC CAACAGTGGC GTACCAGCGA GGAGGGGAAG CTGTAAGCAG CGCAGGCCGG 
CCTGGGCTCT TAAATATTTC TGAGACTGCT AATCAGCCGT GGCTCGCAGA TACCTGGCCA 
AACACTGGAA ACAATCACAA CGAATGCCCC ATCAACTGCT GCACCTCCGG GAATGGCAAC 
AGCGACAGCA ACCTCGCCAC TTACAGCCGC CCAGCTGACT GCATAGCGAA TTACAACAAC 
CAGTTGGACA GCAAGCAGAC AAACCTGATG GTGCCCGACT CGGCTGTTTA TGGGGATGTA 
GACTTAAGCA ACAAGATCAA TGAGATGAAA ACCTTCAACA GCCCCAATCT GAAAGATGGG 
AGGTTTGTCA ATCCTGCCGG CCAGCCCACC CCTTATGCCA CCACTCAGCT CATCCAGTCC 
AACATAAGCA ACAACATCAA CACGTCTGCC GGTGATGTGG ATGAAAAACA CTGGAAACCC 
CCGTGCCAAC AGAAACCGGA TGTTACCCCA ATGCAGTACA ACATCATGGA GCAAAACAAG 
ATCAACAAAG ATTACCGAGC TTCGGACACC ATTCCTTCCA CCATTCCCTA CAACCAGTCC 
CATGATCAGA ATGCCGGAGG GTCATACAAC AGCTCAGACC GGGGAAGTAG CACATCTGGG 
AGCCAAGGTC ATAAGAAGAG TACTCGTACC CCGAAAGTAT CAAAGCAGGC TGGCATGAAC 
TGGGCAGATC TTTTGCCACC ACCACCTGCT CACCCCCCAC CACATGGTGC CACTGAAGAC 
TACACTTTGT CAGCAGATGA AAGCTATGAC AGAGAGTTAC CTTGCCCAAT ACCACCTACA 
AGAATGTATC TTCAGCAAGA CGAACTGGAA GAGGAAGATG AAAGGGGTCC CACACCTCCT 
ATTAGAGGAG CAGCTTCTTC TCCGGCTGCT GTGTCCTACA GCCATCAGTC CACTGCCACT 
CTTACCCCAT CACCTCAGGA CGAACTCCAG CCAATGCTTC AGGACTGTCC CGAGGAGATC 
GGTCATACTC AGCATCAGCT AGAAAGAAGG CGTCATCAAA TTAGTCCTCC TCCACCTCCA 
AGGCCCATTT CCCCGCCTCA TACATATGGT TATATTTCTG GACCAATAGT CTCCGATATG 
GATACTGATG TTCCTGAAGA GGAAGGGGAT GATGGGGAAA TTGAAGTCAC CAAGATGCAA 
AGTCGCAGAC TTCTTTTACG TGGCCTGGAG CAGACTCCTG CTTCCAGTGT TGGAGACCTA 
GAAAGTTCTG TGACTGGTTC CATGATCAAT GGATGGGGCT CAGCTTCTGA AGAAGACAAT 
GTTTCCAGTG GACGTTCCAG TGTCAGTTCA TCGGATGGAT CATTTTTCAC GGATGCGGAT 
TTTGCACAGG CTGTCGCCGC AGCCGCAGAA TATGCTGGGC TTAAAGTAGC AAGGCATCAA 
ATGCAGGAAG CAGCAGGAGG TCGAAGACCT TTCCACGCGT CTCACTGTCC AAGACCAACA 
AGCCCAGTGT CAACAGACAG CAACATGAGC ACCGCTGTCA TACAGAAAAG CCGTCCTGCC 
AAAAAACAAA AACACCAGCA GGGACATCTT CGGAAAGATG TCTACACAGA TGATTTGCCA 
CCACCGCCAC TGCCACCTCC TGCTATCAAA TCTCCAACTG GTCCATCCAA AGCACAACTA 
GAAGGACGGC CTGGCCTTTC ACAGAAGTAC CTTGTTGCAG AACAAAGACT GGAAAGGCCA 
GGAGAGAGGA AAGGTGTCAG CTACAGGGGC AGGGAAGGTC CTGACAGCAG ACAACAACAT 
GCAGAAGCAA GAACCAATTC AGGAGAGAGA AGAGAGTCTT CGGAACAGCA ACTTGATGGA 
AAGATGAGAA AAGGGAAAGC TTCAAAGTGG GATGGGGCAC CTATAGTAGC AAATCAAATA 
CAAGAGGATA TATTACCCTA TTGCAAACCA ACATTCCCCA CTTCAAACAA CCCTCGTGAT 
CCCAGTTCCT CCAGCTCAAT GTCCTCAAGA GGATCTGGTG GACGACGGAG GGGAGAACAA 
ACAACAGGTC GCAGAAATGC AGCAGAGATG CAGCTCTTAG GAAATTATGA ACGAGGGGTT 
GATGAAGATG AAATAGAGGA AACAGAAAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811715.3
CDS	16..5028
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISETANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956261.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956261.3

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGAGCCTCCG AATTTTGTTG TCAAACCTCG TGACCAAGTT 
GTTGCTCTGG GACGCACAGT CACATTTCAG TGTGAAGCCA CCGGAAACCC CCAGCCAGCC 
ATTTTTTGGC AAAAGGAGGG AAGCCAGAAC CTTCTCTTTT CCTACCAACC TCCACAATCG 
TCGAGCCGCT TCTCCGTGTC CCAGACTGGT GACCTGACTA TAACCACCGT TCAGAAGTCA 
GACGTGGGCT ACTACATCTG CCAGACTCTC AACGTTGCCG GGAGCATTAC AACAAAAGCC 
TTCTTAGAAG TTACCGATGT GGTGGCCGAC CGTCCTCCTC CAGTCATTCG TCAGGGACCC 
GTGAATCAGA CAGTAGCTGT TGATGGCACC TTAATAATGA ACTGTATCGC CAGCGGCATT 
CCTGTTCCCA CAATCCACTG GAGAAAGGAC GGGATCCTGA TACCGACCCA AGACTCTCGC 
ATTAAACAGC AGGACTCAGG AGCTCTGCAG ATCCGTTACG CCAAGCTGAG TGATACCGGT 
CGGTACACCT GCACTGCCTC CACTACTAGC GGGGAAGCAA ACTGGAGCGC GTACATAGAA 
GTCCAAGAAT TTGGAGTTCC GGTTCAACCA CCCAGAGCCA CTGATCCCAA CCTGATTCCA 
AGTGCTCCAT CAAAACCAGA AGTCACGGAC GTCAGCAAAA ATACTGTAAC TTTATCCTGG 
CAGCCAAACC TCAACTCAGG TGCTACTCCT ACGTCGTACC TCATAGAGGC CTTCAGCCAT 
GCGTCTGGAA GCAGCTGGCA AACAGTGGCA GAAAATGTCA AGACCGATTC CTTTGCAGTT 
AAAGCACTGA AGCCCAATGC AATCTACCTC TTCCTCGTGA GAGCAGTAAA CGCATACGGA 
TTAAGTGATC CAAGCCAAAT ATCCGATCCT GTAAAAACAC AAGATATTCC TCCTACAAGT 
CAAGGAGTGG ATCACAAACA GGTGCAGAGA GAACTGGGCG ATGTTGTGCT ACACCTTCAT 
AACCCAACAA TACTTTCCTC TTCCTCCATC GAAGTACATT GGACTGTGGA CCAGCAATCA 
CAGTACATCC AAGGATACAA GATCCTCTAC AGGCCAAGCG CAGAGAATCG AGGCGAATCT 
GCATGGTCCA TATTTGAAGT CAGGACTCCG GCCAAAAACA GCGCAGTTAT TCCTGAACTG 
AGAAAAGGCG TTAACTACGA GATCAAAGCC CGCCCCTTTT TTAATGAGTT TCAAGGAGCT 
GATAGTGAAG TGCGGTTCGC TAAGACAATG GAAGAAGCAC CCAGTGCTCC CCCGCAGAGC 
GTTACTGTAA CCAAGAATGA TGGGAATGGA ACTGCTATTG TTGTGGCATG GCAGCCTCCC 
CCTGAAGATA CTCAGAATGG GATGGTCCAG GAATACAAGG TTTGGTGCCT GGGGAATGAG 
AGTCGCTATC ACATAAACAA GACTGTCGAT GGCTCTACCC TTTCCGTGGT CATCCCGTCC 
TTAGTCCCTG GTATCAAATA CAGCGTGGAG GTGGCAGCCA GCACGGGCGC AGGATCCGGT 
GTGAAAAGTG AACCCAACTA CATCCAGTTA GATTCCTTTG GAAACCCGGT GCCTCCGGGG 
GACCAAGTCA GCCTGGCCCA GCAGATATCC GATGTGGTGA AGCAGCCGGC ATTCATAGCA 
GGGATTGGTG CTGCCTGCTG GATAATCCTC ATGGTATTCA GTATCTGGTT GTACCGGCAC 
AGAAAGAAAC GCAATGGGCT CACCAGTTCC TATGCTGGAA TACGGAAAGT CCCGTCTTTT 
ACCTTCACAC CAACAGTGGC GTACCAGCGA GGAGGGGAAG CTGTAAGCAG CGCAGGCCGG 
CCTGGGCTCT TAAATATTTC TGAGACTGCT AATCAGCCGT GGCTCGCAGA TACCTGGCCA 
AACACTGGAA ACAATCACAA CGAATGCCCC ATCAACTGCT GCACCTCCGG GAATGGCAAC 
AGCGACAGCA ACCTCGCCAC TTACAGCCGC CCAGCTGACT GCATAGCGAA TTACAACAAC 
CAGTTGGACA GCAAGCAGAC AAACCTGATG GTGCCCGACT CGGCTGTTTA TGGGGATGTA 
GACTTAAGCA ACAAGATCAA TGAGATGAAA ACCTTCAACA GCCCCAATCT GAAAGATGGG 
AGGTTTGTCA ATCCTGCCGG CCAGCCCACC CCTTATGCCA CCACTCAGCT CATCCAGTCC 
AACATAAGCA ACAACATCAA CACGTCTGCC GGTGATGTGG ATGAAAAACA CTGGAAACCC 
CCGTGCCAAC AGAAACCGGA TGTTACCCCA ATGCAGTACA ACATCATGGA GCAAAACAAG 
ATCAACAAAG ATTACCGAGC TTCGGACACC ATTCCTTCCA CCATTCCCTA CAACCAGTCC 
CATGATCAGA ATGCCGGAGG GTCATACAAC AGCTCAGACC GGGGAAGTAG CACATCTGGG 
AGCCAAGGTC ATAAGAAGAG TACTCGTACC CCGAAAGTAT CAAAGCAGGC TGGCATGAAC 
TGGGCAGATC TTTTGCCACC ACCACCTGCT CACCCCCCAC CACATGGTGC CACTGAAGAC 
TACACTTTGT CAGCAGATGA AAGCTATGAC AGAGAGTTAC CTTGCCCAAT ACCACCTACA 
AGAATGTATC TTCAGCAAGA CGAACTGGAA GAGGAAGATG AAAGGGGTCC CACACCTCCT 
ATTAGAGGAG CAGCTTCTTC TCCGGCTGCT GTGTCCTACA GCCATCAGTC CACTGCCACT 
CTTACCCCAT CACCTCAGGA CGAACTCCAG CCAATGCTTC AGGACTGTCC CGAGGAGATC 
GGTCATACTC AGCATCAGCT AGAAAGAAGG CGTCATCAAA TTAGTCCTCC TCCACCTCCA 
AGGCCCATTT CCCCGCCTCA TACATATGGT TATATTTCTG GACCAATAGT CTCCGATATG 
GATACTGATG TTCCTGAAGA GGAAGGGGAT GATGGGGAAA TTGAAGTCAC CAAGATGCAA 
AGTCGCAGAC TTCTTTTACG TGGCCTGGAG CAGACTCCTG CTTCCAGTGT TGGAGACCTA 
GAAAGTTCTG TGACTGGTTC CATGATCAAT GGATGGGGCT CAGCTTCTGA AGAAGACAAT 
GTTTCCAGTG GACGTTCCAG TGTCAGTTCA TCGGATGGAT CATTTTTCAC GGATGCGGAT 
TTTGCACAGG CTGTCGCCGC AGCCGCAGAA TATGCTGGGC TTAAAGTAGC AAGGCATCAA 
ATGCAGGAAG CAGCAGGAGG TCGAAGACCT TTCCACGCGT CTCACTGTCC AAGACCAACA 
AGCCCAGTGT CAACAGACAG CAACATGAGC ACCGCTGTCA TACAGAAAAG CCGTCCTGCC 
AAAAAACAAA AACACCAGCA GGGACATCTT CGGAAAGATG TCTACACAGA TGATTTGCCA 
CCACCGCCAC TGCCACCTCC TGCTATCAAA TCTCCAACTG GTCCATCCAA AGCACAACTA 
GAAGGACGGC CTGGCCTTTC ACAGAAGTAC CTTGTTGCAG AACAAAGACT GGAAAGGCCA 
GGAGAGAGGA AAGGTGTCAG CTACAGGGGC AGGGAAGGTC CTGACAGCAG ACAACAACAT 
GCAGAAGCAA GAACCAATTC AGGAGAGAGA AGAGAGTCTT CGGAACAGCA ACTTGATGGA 
AAGATGAGAA AAGGGAAAGC TTCAAAGTGG GATGGGGCAC CTATAGTAGC AAATCAAATA 
CAAGAGGATA TATTACCCTA TTGCAAACCA ACATTCCCCA CTTCAAACAA CCCTCGTGAT 
CCCAGTTCCT CCAGCTCAAT GTCCTCAAGA GGATCTGGTG GACGACGGAG GGGAGAACAA 
ACAACAGGTC GCAGAAATGC AGCAGAGATG CAGCTCTTAG GAAATTATGA ACGAGGGGTT 
GATGAAGATG AAATAGAGGA AACAGAAAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956262.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811716.3 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X6, mRNA.

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CloneID	OXa51067
Clone ID Related Accession (Same CDS sequence)	XM_012956262.3
Accession Version	XM_012956262.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956262.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTG 
GCGTACCAGC GAGGAGGGGA AGCTGTAAGC AGCGCAGGCC GGCCTGGGCT CTTAAATATT 
TCTGAGACTG CTAATCAGCC GTGGCTCGCA GATACCTGGC CAAACACTGG AAACAATCAC 
AACGAATGCC CCATCAACTG CTGCACCTCC GGGAATGGCA ACAGCGACAG CAACCTCGCC 
ACTTACAGCC GCCCAGCTGA CTGCATAGCG AATTACAACA ACCAGTTGGA CAGCAAGCAG 
ACAAACCTGA TGGTGCCCGA CTCGGCTGTT TATGGGGATG TAGACTTAAG CAACAAGATC 
AATGAGATGA AAACCTTCAA CAGCCCCAAT CTGAAAGATG GGAGGTTTGT CAATCCTGCC 
GGCCAGCCCA CCCCTTATGC CACCACTCAG CTCATCCAGT CCAACATAAG CAACAACATC 
AACACGTCTG CCGGTGATGT GGATGAAAAA CACTGGAAAC CCCCGTGCCA ACAGAAACCG 
GATGTTACCC CAATGCAGTA CAACATCATG GAGCAAAACA AGATCAACAA AGATTACCGA 
GCTTCGGACA CCATTCCTTC CACCATTCCC TACAACCAGT CCCATGATCA GAATGCCGGA 
GGGTCATACA ACAGCTCAGA CCGGGGAAGT AGCACATCTG GGAGCCAAGG TCATAAGAAG 
AGTACTCGTA CCCCGAAAGT ATCAAAGCAG GCTGGCATGA ACTGGGCAGA TCTTTTGCCA 
CCACCACCTG CTCACCCCCC ACCACATGGT GCCACTGAAG ACTACACTTT GTCAGCAGAT 
GAAAGCTATG ACAGAGAGTT ACCTTGCCCA ATACCACCTA CAAGAATGTA TCTTCAGCAA 
GACGAACTGG AAGAGGAAGA TGAAAGGGGT CCCACACCTC CTATTAGAGG AGCAGCTTCT 
TCTCCGGCTG CTGTGTCCTA CAGCCATCAG TCCACTGCCA CTCTTACCCC ATCACCTCAG 
GACGAACTCC AGCCAATGCT TCAGGACTGT CCCGAGGAGA TCGGTCATAC TCAGCATCAG 
CTAGAAAGAA GGCGTCATCA AATTAGTCCT CCTCCACCTC CAAGGCCCAT TTCCCCGCCT 
CATACATATG GTTATATTTC TGGACCAATA GTCTCCGATA TGGATACTGA TGTTCCTGAA 
GAGGAAGGGG ATGATGGGGA AATTGAAGTC ACCAAGATGC AAAGTCGCAG ACTTCTTTTA 
CGTGGCCTGG AGCAGACTCC TGCTTCCAGT GTTGGAGACC TAGAAAGTTC TGTGACTGGT 
TCCATGATCA ATGGATGGGG CTCAGCTTCT GAAGAAGACA ATGTTTCCAG TGGACGTTCC 
AGTGTCAGTT CATCGGATGG ATCATTTTTC ACGGATGCGG ATTTTGCACA GGCTGTCGCC 
GCAGCCGCAG AATATGCTGG GCTTAAAGTA GCAAGGCATC AAATGCAGGA AGCAGCAGGA 
GGTCGAAGAC CTTTCCACGC GTCTCACTGT CCAAGACCAA CAAGCCCAGT GTCAACAGAC 
AGCAACATGA GCACCGCTGT CATACAGAAA AGCCGTCCTG CCAAAAAACA AAAACACCAG 
CAGGGACATC TTCGGAAAGA TGTCTACACA GATGATTTGC CACCACCGCC ACTGCCACCT 
CCTGCTATCA AATCTCCAAC TGGTCCATCC AAAGCACAAC TAGAAGGACG GCCTGGCCTT 
TCACAGAAGT ACCTTGTTGC AGAACAAAGA CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC 
AGCTACAGGG GCAGGGAAGG TCCTGACAGC AGACAACAAC ATGCAGAAGC AAGAACCAAT 
TCAGGAGAGA GAAGAGAGTC TTCGGAACAG CAACTTGATG GAAAGATGAG AAAAGGGAAA 
GCTTCAAAGT GGGATGGGGC ACCTATAGTA GCAAATCAAA TACAAGAGGA TATATTACCC 
TATTGCAAAC CAACATTCCC CACTTCAAAC AACCCTCGTG ATCCCAGTTC CTCCAGCTCA 
ATGTCCTCAA GAGGATCTGG TGGACGACGG AGGGGAGAAC AAACAACAGG TCGCAGAAAT 
GCAGCAGAGA TGCAGCTCTT AGGAAATTAT GAACGAGGGG TTGATGAAGA TGAAATAGAG 
GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811716.3
CDS	16..5010
Translation	MMRLPSLLLLHVFLPCMDLHVTGNVWNKIAFSLATVTPSAALIYFSTDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVAYQRGGEAVSSAGRPGLLNISETANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956262.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956262.3

ORF Insert Sequence:

ATGATGAGAC TTCCATCTCT GCTGTTGCTT CATGTCTTCC TTCCATGCAT GGACCTACAT 
GTAACTGGAA ATGTTTGGAA CAAAATTGCA TTTTCTCTTG CCACTGTGAC CCCATCTGCG 
GCTTTGATAT ATTTTTCAAC AGACTCCGAT GATCTTGACC GGGGGTTGGA CAGCGGGACG 
GCACTTCCTA CATCTGATCA TGATGACAAT TCGCTGGTCT ATACAGGGTC ACGTCTTCGG 
CAAGAAGATT TCCCACCGCG AATTGTTGAG CATCCCTCAG ACCTAATTGT CTCCAAAGGT 
GAACCAGCAA CGCTGAACTG CAAGGCCGAA GGGAGGCCGA CTCCAACCAT TGAGTGGTAT 
AAAGGAGGAG ACAAAGTAGA GACAGATAAA GACGATCCTC GCTCTCACCG AATGTTGTTG 
CCCAGTGGAT CACTATTTTT TTTACGTATC GTGCATGGAC GCAAAATCAG GCCGGATGAA 
GGGGTCTATG TTTGTGTTGC AAGGAATTAC CTCGGAGAAG CTGTGAGCCA CAATGCATCG 
CTGGAAGTTG CAATTTTGAG AGACGACTTC AGACAAAACC CTTCAGATGT AATGGTAGCG 
GTGGGGGAGC CTGCAGTAAT GGAATGCCAG CCGCCCAGAG GTCATCCTGA GCCGACAATA 
TCCTGGAAAA AAGATGGGAC GCCAATAGAC GATGATAAGG ATGAAAGAAT AACTATAAGA 
GGAGGGAAAC TTATGATCAC CTACACCAGG AAAAGCGATG CTGGAAAATA TGTGTGTGTT 
GGAACCAATA TGGTGGGAGA ACGGGAAAGC GAAGTAGCAG AACTTACTGT TTTAGAAAGG 
CCATCATTTG TGAGGAGACC CAGTAATATG GCAGTTACCG TAGATGATAG CGTTGAATTC 
AAATGTGAGG CCCGGGGTGA TCCTGTCCCT ACTGTTCGAT GGAGGAAAGA TGATGGGGAT 
CTGCCTAAAT CAAGATATGA AATCCGTGAT GACCACACCT TGAAGATAAG GAAGGTCACA 
GCAGGGGACA TGGGATCCTA TACTTGTGTT GCAGAGAACA TGGTTGGAAA AGCGGAAGCT 
TCGGCCGTCC TAACAGTTCA AGTTGTGTCT GAGCCTCCGA ATTTTGTTGT CAAACCTCGT 
GACCAAGTTG TTGCTCTGGG ACGCACAGTC ACATTTCAGT GTGAAGCCAC CGGAAACCCC 
CAGCCAGCCA TTTTTTGGCA AAAGGAGGGA AGCCAGAACC TTCTCTTTTC CTACCAACCT 
CCACAATCGT CGAGCCGCTT CTCCGTGTCC CAGACTGGTG ACCTGACTAT AACCACCGTT 
CAGAAGTCAG ACGTGGGCTA CTACATCTGC CAGACTCTCA ACGTTGCCGG GAGCATTACA 
ACAAAAGCCT TCTTAGAAGT TACCGATGTG GTGGCCGACC GTCCTCCTCC AGTCATTCGT 
CAGGGACCCG TGAATCAGAC AGTAGCTGTT GATGGCACCT TAATAATGAA CTGTATCGCC 
AGCGGCATTC CTGTTCCCAC AATCCACTGG AGAAAGGACG GGATCCTGAT ACCGACCCAA 
GACTCTCGCA TTAAACAGCA GGACTCAGGA GCTCTGCAGA TCCGTTACGC CAAGCTGAGT 
GATACCGGTC GGTACACCTG CACTGCCTCC ACTACTAGCG GGGAAGCAAA CTGGAGCGCG 
TACATAGAAG TCCAAGAATT TGGAGTTCCG GTTCAACCAC CCAGAGCCAC TGATCCCAAC 
CTGATTCCAA GTGCTCCATC AAAACCAGAA GTCACGGACG TCAGCAAAAA TACTGTAACT 
TTATCCTGGC AGCCAAACCT CAACTCAGGT GCTACTCCTA CGTCGTACCT CATAGAGGCC 
TTCAGCCATG CGTCTGGAAG CAGCTGGCAA ACAGTGGCAG AAAATGTCAA GACCGATTCC 
TTTGCAGTTA AAGCACTGAA GCCCAATGCA ATCTACCTCT TCCTCGTGAG AGCAGTAAAC 
GCATACGGAT TAAGTGATCC AAGCCAAATA TCCGATCCTG TAAAAACACA AGATATTCCT 
CCTACAAGTC AAGGAGTGGA TCACAAACAG GTGCAGAGAG AACTGGGCGA TGTTGTGCTA 
CACCTTCATA ACCCAACAAT ACTTTCCTCT TCCTCCATCG AAGTACATTG GACTGTGGAC 
CAGCAATCAC AGTACATCCA AGGATACAAG ATCCTCTACA GGCCAAGCGC AGAGAATCGA 
GGCGAATCTG CATGGTCCAT ATTTGAAGTC AGGACTCCGG CCAAAAACAG CGCAGTTATT 
CCTGAACTGA GAAAAGGCGT TAACTACGAG ATCAAAGCCC GCCCCTTTTT TAATGAGTTT 
CAAGGAGCTG ATAGTGAAGT GCGGTTCGCT AAGACAATGG AAGAAGCACC CAGTGCTCCC 
CCGCAGAGCG TTACTGTAAC CAAGAATGAT GGGAATGGAA CTGCTATTGT TGTGGCATGG 
CAGCCTCCCC CTGAAGATAC TCAGAATGGG ATGGTCCAGG AATACAAGGT TTGGTGCCTG 
GGGAATGAGA GTCGCTATCA CATAAACAAG ACTGTCGATG GCTCTACCCT TTCCGTGGTC 
ATCCCGTCCT TAGTCCCTGG TATCAAATAC AGCGTGGAGG TGGCAGCCAG CACGGGCGCA 
GGATCCGGTG TGAAAAGTGA ACCCAACTAC ATCCAGTTAG ATTCCTTTGG AAACCCGGTG 
CCTCCGGGGG ACCAAGTCAG CCTGGCCCAG CAGATATCCG ATGTGGTGAA GCAGCCGGCA 
TTCATAGCAG GGATTGGTGC TGCCTGCTGG ATAATCCTCA TGGTATTCAG TATCTGGTTG 
TACCGGCACA GAAAGAAACG CAATGGGCTC ACCAGTTCCT ATGCTGGAAT ACGGAAAGTG 
GCGTACCAGC GAGGAGGGGA AGCTGTAAGC AGCGCAGGCC GGCCTGGGCT CTTAAATATT 
TCTGAGACTG CTAATCAGCC GTGGCTCGCA GATACCTGGC CAAACACTGG AAACAATCAC 
AACGAATGCC CCATCAACTG CTGCACCTCC GGGAATGGCA ACAGCGACAG CAACCTCGCC 
ACTTACAGCC GCCCAGCTGA CTGCATAGCG AATTACAACA ACCAGTTGGA CAGCAAGCAG 
ACAAACCTGA TGGTGCCCGA CTCGGCTGTT TATGGGGATG TAGACTTAAG CAACAAGATC 
AATGAGATGA AAACCTTCAA CAGCCCCAAT CTGAAAGATG GGAGGTTTGT CAATCCTGCC 
GGCCAGCCCA CCCCTTATGC CACCACTCAG CTCATCCAGT CCAACATAAG CAACAACATC 
AACACGTCTG CCGGTGATGT GGATGAAAAA CACTGGAAAC CCCCGTGCCA ACAGAAACCG 
GATGTTACCC CAATGCAGTA CAACATCATG GAGCAAAACA AGATCAACAA AGATTACCGA 
GCTTCGGACA CCATTCCTTC CACCATTCCC TACAACCAGT CCCATGATCA GAATGCCGGA 
GGGTCATACA ACAGCTCAGA CCGGGGAAGT AGCACATCTG GGAGCCAAGG TCATAAGAAG 
AGTACTCGTA CCCCGAAAGT ATCAAAGCAG GCTGGCATGA ACTGGGCAGA TCTTTTGCCA 
CCACCACCTG CTCACCCCCC ACCACATGGT GCCACTGAAG ACTACACTTT GTCAGCAGAT 
GAAAGCTATG ACAGAGAGTT ACCTTGCCCA ATACCACCTA CAAGAATGTA TCTTCAGCAA 
GACGAACTGG AAGAGGAAGA TGAAAGGGGT CCCACACCTC CTATTAGAGG AGCAGCTTCT 
TCTCCGGCTG CTGTGTCCTA CAGCCATCAG TCCACTGCCA CTCTTACCCC ATCACCTCAG 
GACGAACTCC AGCCAATGCT TCAGGACTGT CCCGAGGAGA TCGGTCATAC TCAGCATCAG 
CTAGAAAGAA GGCGTCATCA AATTAGTCCT CCTCCACCTC CAAGGCCCAT TTCCCCGCCT 
CATACATATG GTTATATTTC TGGACCAATA GTCTCCGATA TGGATACTGA TGTTCCTGAA 
GAGGAAGGGG ATGATGGGGA AATTGAAGTC ACCAAGATGC AAAGTCGCAG ACTTCTTTTA 
CGTGGCCTGG AGCAGACTCC TGCTTCCAGT GTTGGAGACC TAGAAAGTTC TGTGACTGGT 
TCCATGATCA ATGGATGGGG CTCAGCTTCT GAAGAAGACA ATGTTTCCAG TGGACGTTCC 
AGTGTCAGTT CATCGGATGG ATCATTTTTC ACGGATGCGG ATTTTGCACA GGCTGTCGCC 
GCAGCCGCAG AATATGCTGG GCTTAAAGTA GCAAGGCATC AAATGCAGGA AGCAGCAGGA 
GGTCGAAGAC CTTTCCACGC GTCTCACTGT CCAAGACCAA CAAGCCCAGT GTCAACAGAC 
AGCAACATGA GCACCGCTGT CATACAGAAA AGCCGTCCTG CCAAAAAACA AAAACACCAG 
CAGGGACATC TTCGGAAAGA TGTCTACACA GATGATTTGC CACCACCGCC ACTGCCACCT 
CCTGCTATCA AATCTCCAAC TGGTCCATCC AAAGCACAAC TAGAAGGACG GCCTGGCCTT 
TCACAGAAGT ACCTTGTTGC AGAACAAAGA CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC 
AGCTACAGGG GCAGGGAAGG TCCTGACAGC AGACAACAAC ATGCAGAAGC AAGAACCAAT 
TCAGGAGAGA GAAGAGAGTC TTCGGAACAG CAACTTGATG GAAAGATGAG AAAAGGGAAA 
GCTTCAAAGT GGGATGGGGC ACCTATAGTA GCAAATCAAA TACAAGAGGA TATATTACCC 
TATTGCAAAC CAACATTCCC CACTTCAAAC AACCCTCGTG ATCCCAGTTC CTCCAGCTCA 
ATGTCCTCAA GAGGATCTGG TGGACGACGG AGGGGAGAAC AAACAACAGG TCGCAGAAAT 
GCAGCAGAGA TGCAGCTCTT AGGAAATTAT GAACGAGGGG TTGATGAAGA TGAAATAGAG 
GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956257.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811711.2 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X7, mRNA.

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CloneID	OXa51068
Clone ID Related Accession (Same CDS sequence)	XM_012956257.3
Accession Version	XM_012956257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTTC CCAACACTGC TGCTATGTTT CTGGTGGCAC TGTCTGGTCT ACTTTTCCAG 
CCATTGGTGG GACAACTGAT TCCAGACTCC GATGATCTTG ACCGGGGGTT GGACAGCGGG 
ACGGCACTTC CTACATCTGA TCATGATGAC AATTCGCTGG TCTATACAGG GTCACGTCTT 
CGGCAAGAAG ATTTCCCACC GCGAATTGTT GAGCATCCCT CAGACCTAAT TGTCTCCAAA 
GGTGAACCAG CAACGCTGAA CTGCAAGGCC GAAGGGAGGC CGACTCCAAC CATTGAGTGG 
TATAAAGGAG GAGACAAAGT AGAGACAGAT AAAGACGATC CTCGCTCTCA CCGAATGTTG 
TTGCCCAGTG GATCACTATT TTTTTTACGT ATCGTGCATG GACGCAAAAT CAGGCCGGAT 
GAAGGGGTCT ATGTTTGTGT TGCAAGGAAT TACCTCGGAG AAGCTGTGAG CCACAATGCA 
TCGCTGGAAG TTGCAATTTT GAGAGACGAC TTCAGACAAA ACCCTTCAGA TGTAATGGTA 
GCGGTGGGGG AGCCTGCAGT AATGGAATGC CAGCCGCCCA GAGGTCATCC TGAGCCGACA 
ATATCCTGGA AAAAAGATGG GACGCCAATA GACGATGATA AGGATGAAAG AATAACTATA 
AGAGGAGGGA AACTTATGAT CACCTACACC AGGAAAAGCG ATGCTGGAAA ATATGTGTGT 
GTTGGAACCA ATATGGTGGG AGAACGGGAA AGCGAAGTAG CAGAACTTAC TGTTTTAGAA 
AGGCCATCAT TTGTGAGGAG ACCCAGTAAT ATGGCAGTTA CCGTAGATGA TAGCGTTGAA 
TTCAAATGTG AGGCCCGGGG TGATCCTGTC CCTACTGTTC GATGGAGGAA AGATGATGGG 
GATCTGCCTA AATCAAGATA TGAAATCCGT GATGACCACA CCTTGAAGAT AAGGAAGGTC 
ACAGCAGGGG ACATGGGATC CTATACTTGT GTTGCAGAGA ACATGGTTGG AAAAGCGGAA 
GCTTCGGCCG TCCTAACAGT TCAAGTTGTG TCTGAGCCTC CGAATTTTGT TGTCAAACCT 
CGTGACCAAG TTGTTGCTCT GGGACGCACA GTCACATTTC AGTGTGAAGC CACCGGAAAC 
CCCCAGCCAG CCATTTTTTG GCAAAAGGAG GGAAGCCAGA ACCTTCTCTT TTCCTACCAA 
CCTCCACAAT CGTCGAGCCG CTTCTCCGTG TCCCAGACTG GTGACCTGAC TATAACCACC 
GTTCAGAAGT CAGACGTGGG CTACTACATC TGCCAGACTC TCAACGTTGC CGGGAGCATT 
ACAACAAAAG CCTTCTTAGA AGTTACCGAT GTGGTGGCCG ACCGTCCTCC TCCAGTCATT 
CGTCAGGGAC CCGTGAATCA GACAGTAGCT GTTGATGGCA CCTTAATAAT GAACTGTATC 
GCCAGCGGCA TTCCTGTTCC CACAATCCAC TGGAGAAAGG ACGGGATCCT GATACCGACC 
CAAGACTCTC GCATTAAACA GCAGGACTCA GGAGCTCTGC AGATCCGTTA CGCCAAGCTG 
AGTGATACCG GTCGGTACAC CTGCACTGCC TCCACTACTA GCGGGGAAGC AAACTGGAGC 
GCGTACATAG AAGTCCAAGA ATTTGGAGTT CCGGTTCAAC CACCCAGAGC CACTGATCCC 
AACCTGATTC CAAGTGCTCC ATCAAAACCA GAAGTCACGG ACGTCAGCAA AAATACTGTA 
ACTTTATCCT GGCAGCCAAA CCTCAACTCA GGTGCTACTC CTACGTCGTA CCTCATAGAG 
GCCTTCAGCC ATGCGTCTGG AAGCAGCTGG CAAACAGTGG CAGAAAATGT CAAGACCGAT 
TCCTTTGCAG TTAAAGCACT GAAGCCCAAT GCAATCTACC TCTTCCTCGT GAGAGCAGTA 
AACGCATACG GATTAAGTGA TCCAAGCCAA ATATCCGATC CTGTAAAAAC ACAAGATATT 
CCTCCTACAA GTCAAGGAGT GGATCACAAA CAGGTGCAGA GAGAACTGGG CGATGTTGTG 
CTACACCTTC ATAACCCAAC AATACTTTCC TCTTCCTCCA TCGAAGTACA TTGGACTGTG 
GACCAGCAAT CACAGTACAT CCAAGGATAC AAGATCCTCT ACAGGCCAAG CGCAGAGAAT 
CGAGGCGAAT CTGCATGGTC CATATTTGAA GTCAGGACTC CGGCCAAAAA CAGCGCAGTT 
ATTCCTGAAC TGAGAAAAGG CGTTAACTAC GAGATCAAAG CCCGCCCCTT TTTTAATGAG 
TTTCAAGGAG CTGATAGTGA AGTGCGGTTC GCTAAGACAA TGGAAGAAGC ACCCAGTGCT 
CCCCCGCAGA GCGTTACTGT AACCAAGAAT GATGGGAATG GAACTGCTAT TGTTGTGGCA 
TGGCAGCCTC CCCCTGAAGA TACTCAGAAT GGGATGGTCC AGGAATACAA GGTTTGGTGC 
CTGGGGAATG AGAGTCGCTA TCACATAAAC AAGACTGTCG ATGGCTCTAC CCTTTCCGTG 
GTCATCCCGT CCTTAGTCCC TGGTATCAAA TACAGCGTGG AGGTGGCAGC CAGCACGGGC 
GCAGGATCCG GTGTGAAAAG TGAACCCAAC TACATCCAGT TAGATTCCTT TGGAAACCCG 
GTGCCTCCGG GGGACCAAGT CAGCCTGGCC CAGCAGATAT CCGATGTGGT GAAGCAGCCG 
GCATTCATAG CAGGGATTGG TGCTGCCTGC TGGATAATCC TCATGGTATT CAGTATCTGG 
TTGTACCGGC ACAGAAAGAA ACGCAATGGG CTCACCAGTT CCTATGCTGG AATACGGAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTG GCGTACCAGC GAGGAGGGGA AGCTGTAAGC 
AGCGCAGGCC GGCCTGGGCT CTTAAATATT TCTGAGACTG CTAATCAGCC GTGGCTCGCA 
GATACCTGGC CAAACACTGG AAACAATCAC AACGAATGCC CCATCAACTG CTGCACCTCC 
GGGAATGGCA ACAGCGACAG CAACCTCGCC ACTTACAGCC GCCCAGCTGA CTGCATAGCG 
AATTACAACA ACCAGTTGGA CAGCAAGCAG ACAAACCTGA TGGTGCCCGA CTCGGCTGTT 
TATGGGGATG TAGACTTAAG CAACAAGATC AATGAGATGA AAACCTTCAA CAGCCCCAAT 
CTGAAAGATG GGAGGTTTGT CAATCCTGCC GGCCAGCCCA CCCCTTATGC CACCACTCAG 
CTCATCCAGT CCAACATAAG CAACAACATC AACACGTCTG CCGGTGATGT GGATGAAAAA 
CACTGGAAAC CCCCGTGCCA ACAGAAACCG GATGTTACCC CAATGCAGTA CAACATCATG 
GAGCAAAACA AGATCAACAA AGATTACCGA GCTTCGGACA CCATTCCTTC CACCATTCCC 
TACAACCAGT CCCATGATCA GAATGCCGGA GGGTCATACA ACAGCTCAGA CCGGGGAAGT 
AGCACATCTG GGAGCCAAGG TCATAAGAAG AGTACTCGTA CCCCGAAAGT ATCAAAGCAG 
GCTGGCATGA ACTGGGCAGA TCTTTTGCCA CCACCACCTG CTCACCCCCC ACCACATGGT 
GCCACTGAAG ACTACACTTT GTCAGCAGAT GAAAGCTATG ACAGAGAGTT ACCTTGCCCA 
ATACCACCTA CAAGAATGTA TCTTCAGCAA GACGAACTGG AAGAGGAAGA TGAAAGGGGT 
CCCACACCTC CTATTAGAGG AGCAGCTTCT TCTCCGGCTG CTGTGTCCTA CAGCCATCAG 
TCCACTGCCA CTCTTACCCC ATCACCTCAG GACGAACTCC AGCCAATGCT TCAGGACTGT 
CCCGAGGAGA TCGGTCATAC TCAGCATCAG CTAGAAAGAA GGCGTCATCA AATTAGTCCT 
CCTCCACCTC CAAGGCCCAT TTCCCCGCCT CATACATATG GTTATATTTC TGGACCAATA 
GTCTCCGATA TGGATACTGA TGTTCCTGAA GAGGAAGGGG ATGATGGGGA AATTGAAGTC 
ACCAAGATGC AAAGTCGCAG ACTTCTTTTA CGTGGCCTGG AGCAGACTCC TGCTTCCAGT 
GTTGGAGACC TAGAAAGTTC TGTGACTGGT TCCATGATCA ATGGATGGGG CTCAGCTTCT 
GAAGAAGACA ATGTTTCCAG TGGACGTTCC AGTGTCAGTT CATCGGATGG ATCATTTTTC 
ACGGATGCGG ATTTTGCACA GGCTGTCGCC GCAGCCGCAG AATATGCTGG GCTTAAAGTA 
GCAAGGCATC AAATGCAGGA AGCAGCAGGA GTGTCGCGTC TTTGCCTCCC AGGTCGAAGA 
CCTTTCCACG CGTCTCACTG TCCAAGACCA ACAAGCCCAG TGTCAACAGA CAGCAACATG 
AGCACCGCTG TCATACAGAA AAGCCGTCCT GCCAAAAAAC AAAAACACCA GCAGGGACAT 
CTTCGGAAAG ATGTCTACAC AGATGATTTG CCACCACCGC CACTGCCACC TCCTGCTATC 
AAATCTCCAA CTGGTCCATC CAAAGCACAA CTAGAAGGAC GGCCTGGCCT TTCACAGAAG 
TACCTTGTTG CAGAACAAAG ACTGGAAAGG CCAGGAGAGA GGAAAGGTGT CAGCTACAGG 
GGCAGGGAAG GTCCTGACAG CAGACAACAA CATGCAGAAG CAAGAACCAA TTCAGGAGAG 
AGAAGAGAGT CTTCGGAACA GCAACTTGAT GGAAAGATGA GAAAAGGGAA AGCTTCAAAG 
TGGGATGGGG CACCTATAGT AGCAAATCAA ATACAAGAGG ATATATTACC CTATTGCAAA 
CCAACATTCC CCACTTCAAA CAACCCTCGT GATCCCAGTT CCTCCAGCTC AATGTCCTCA 
AGAGGATCTG GTGGACGACG GAGGGGAGAA CAAACAACAG GTCGCAGAAA TGCAGCAGAG 
ATGCAGCTCT TAGGAAATTA TGAACGAGGG GTTGATGAAG ATGAAATAGA GGAAACAGAA 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811711.2
CDS	262..5247
Translation	MKLPNTAAMFLVALSGLLFQPLVGQLIPDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISETANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956257.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956257.3

ORF Insert Sequence:

ATGAAACTTC CCAACACTGC TGCTATGTTT CTGGTGGCAC TGTCTGGTCT ACTTTTCCAG 
CCATTGGTGG GACAACTGAT TCCAGACTCC GATGATCTTG ACCGGGGGTT GGACAGCGGG 
ACGGCACTTC CTACATCTGA TCATGATGAC AATTCGCTGG TCTATACAGG GTCACGTCTT 
CGGCAAGAAG ATTTCCCACC GCGAATTGTT GAGCATCCCT CAGACCTAAT TGTCTCCAAA 
GGTGAACCAG CAACGCTGAA CTGCAAGGCC GAAGGGAGGC CGACTCCAAC CATTGAGTGG 
TATAAAGGAG GAGACAAAGT AGAGACAGAT AAAGACGATC CTCGCTCTCA CCGAATGTTG 
TTGCCCAGTG GATCACTATT TTTTTTACGT ATCGTGCATG GACGCAAAAT CAGGCCGGAT 
GAAGGGGTCT ATGTTTGTGT TGCAAGGAAT TACCTCGGAG AAGCTGTGAG CCACAATGCA 
TCGCTGGAAG TTGCAATTTT GAGAGACGAC TTCAGACAAA ACCCTTCAGA TGTAATGGTA 
GCGGTGGGGG AGCCTGCAGT AATGGAATGC CAGCCGCCCA GAGGTCATCC TGAGCCGACA 
ATATCCTGGA AAAAAGATGG GACGCCAATA GACGATGATA AGGATGAAAG AATAACTATA 
AGAGGAGGGA AACTTATGAT CACCTACACC AGGAAAAGCG ATGCTGGAAA ATATGTGTGT 
GTTGGAACCA ATATGGTGGG AGAACGGGAA AGCGAAGTAG CAGAACTTAC TGTTTTAGAA 
AGGCCATCAT TTGTGAGGAG ACCCAGTAAT ATGGCAGTTA CCGTAGATGA TAGCGTTGAA 
TTCAAATGTG AGGCCCGGGG TGATCCTGTC CCTACTGTTC GATGGAGGAA AGATGATGGG 
GATCTGCCTA AATCAAGATA TGAAATCCGT GATGACCACA CCTTGAAGAT AAGGAAGGTC 
ACAGCAGGGG ACATGGGATC CTATACTTGT GTTGCAGAGA ACATGGTTGG AAAAGCGGAA 
GCTTCGGCCG TCCTAACAGT TCAAGTTGTG TCTGAGCCTC CGAATTTTGT TGTCAAACCT 
CGTGACCAAG TTGTTGCTCT GGGACGCACA GTCACATTTC AGTGTGAAGC CACCGGAAAC 
CCCCAGCCAG CCATTTTTTG GCAAAAGGAG GGAAGCCAGA ACCTTCTCTT TTCCTACCAA 
CCTCCACAAT CGTCGAGCCG CTTCTCCGTG TCCCAGACTG GTGACCTGAC TATAACCACC 
GTTCAGAAGT CAGACGTGGG CTACTACATC TGCCAGACTC TCAACGTTGC CGGGAGCATT 
ACAACAAAAG CCTTCTTAGA AGTTACCGAT GTGGTGGCCG ACCGTCCTCC TCCAGTCATT 
CGTCAGGGAC CCGTGAATCA GACAGTAGCT GTTGATGGCA CCTTAATAAT GAACTGTATC 
GCCAGCGGCA TTCCTGTTCC CACAATCCAC TGGAGAAAGG ACGGGATCCT GATACCGACC 
CAAGACTCTC GCATTAAACA GCAGGACTCA GGAGCTCTGC AGATCCGTTA CGCCAAGCTG 
AGTGATACCG GTCGGTACAC CTGCACTGCC TCCACTACTA GCGGGGAAGC AAACTGGAGC 
GCGTACATAG AAGTCCAAGA ATTTGGAGTT CCGGTTCAAC CACCCAGAGC CACTGATCCC 
AACCTGATTC CAAGTGCTCC ATCAAAACCA GAAGTCACGG ACGTCAGCAA AAATACTGTA 
ACTTTATCCT GGCAGCCAAA CCTCAACTCA GGTGCTACTC CTACGTCGTA CCTCATAGAG 
GCCTTCAGCC ATGCGTCTGG AAGCAGCTGG CAAACAGTGG CAGAAAATGT CAAGACCGAT 
TCCTTTGCAG TTAAAGCACT GAAGCCCAAT GCAATCTACC TCTTCCTCGT GAGAGCAGTA 
AACGCATACG GATTAAGTGA TCCAAGCCAA ATATCCGATC CTGTAAAAAC ACAAGATATT 
CCTCCTACAA GTCAAGGAGT GGATCACAAA CAGGTGCAGA GAGAACTGGG CGATGTTGTG 
CTACACCTTC ATAACCCAAC AATACTTTCC TCTTCCTCCA TCGAAGTACA TTGGACTGTG 
GACCAGCAAT CACAGTACAT CCAAGGATAC AAGATCCTCT ACAGGCCAAG CGCAGAGAAT 
CGAGGCGAAT CTGCATGGTC CATATTTGAA GTCAGGACTC CGGCCAAAAA CAGCGCAGTT 
ATTCCTGAAC TGAGAAAAGG CGTTAACTAC GAGATCAAAG CCCGCCCCTT TTTTAATGAG 
TTTCAAGGAG CTGATAGTGA AGTGCGGTTC GCTAAGACAA TGGAAGAAGC ACCCAGTGCT 
CCCCCGCAGA GCGTTACTGT AACCAAGAAT GATGGGAATG GAACTGCTAT TGTTGTGGCA 
TGGCAGCCTC CCCCTGAAGA TACTCAGAAT GGGATGGTCC AGGAATACAA GGTTTGGTGC 
CTGGGGAATG AGAGTCGCTA TCACATAAAC AAGACTGTCG ATGGCTCTAC CCTTTCCGTG 
GTCATCCCGT CCTTAGTCCC TGGTATCAAA TACAGCGTGG AGGTGGCAGC CAGCACGGGC 
GCAGGATCCG GTGTGAAAAG TGAACCCAAC TACATCCAGT TAGATTCCTT TGGAAACCCG 
GTGCCTCCGG GGGACCAAGT CAGCCTGGCC CAGCAGATAT CCGATGTGGT GAAGCAGCCG 
GCATTCATAG CAGGGATTGG TGCTGCCTGC TGGATAATCC TCATGGTATT CAGTATCTGG 
TTGTACCGGC ACAGAAAGAA ACGCAATGGG CTCACCAGTT CCTATGCTGG AATACGGAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTG GCGTACCAGC GAGGAGGGGA AGCTGTAAGC 
AGCGCAGGCC GGCCTGGGCT CTTAAATATT TCTGAGACTG CTAATCAGCC GTGGCTCGCA 
GATACCTGGC CAAACACTGG AAACAATCAC AACGAATGCC CCATCAACTG CTGCACCTCC 
GGGAATGGCA ACAGCGACAG CAACCTCGCC ACTTACAGCC GCCCAGCTGA CTGCATAGCG 
AATTACAACA ACCAGTTGGA CAGCAAGCAG ACAAACCTGA TGGTGCCCGA CTCGGCTGTT 
TATGGGGATG TAGACTTAAG CAACAAGATC AATGAGATGA AAACCTTCAA CAGCCCCAAT 
CTGAAAGATG GGAGGTTTGT CAATCCTGCC GGCCAGCCCA CCCCTTATGC CACCACTCAG 
CTCATCCAGT CCAACATAAG CAACAACATC AACACGTCTG CCGGTGATGT GGATGAAAAA 
CACTGGAAAC CCCCGTGCCA ACAGAAACCG GATGTTACCC CAATGCAGTA CAACATCATG 
GAGCAAAACA AGATCAACAA AGATTACCGA GCTTCGGACA CCATTCCTTC CACCATTCCC 
TACAACCAGT CCCATGATCA GAATGCCGGA GGGTCATACA ACAGCTCAGA CCGGGGAAGT 
AGCACATCTG GGAGCCAAGG TCATAAGAAG AGTACTCGTA CCCCGAAAGT ATCAAAGCAG 
GCTGGCATGA ACTGGGCAGA TCTTTTGCCA CCACCACCTG CTCACCCCCC ACCACATGGT 
GCCACTGAAG ACTACACTTT GTCAGCAGAT GAAAGCTATG ACAGAGAGTT ACCTTGCCCA 
ATACCACCTA CAAGAATGTA TCTTCAGCAA GACGAACTGG AAGAGGAAGA TGAAAGGGGT 
CCCACACCTC CTATTAGAGG AGCAGCTTCT TCTCCGGCTG CTGTGTCCTA CAGCCATCAG 
TCCACTGCCA CTCTTACCCC ATCACCTCAG GACGAACTCC AGCCAATGCT TCAGGACTGT 
CCCGAGGAGA TCGGTCATAC TCAGCATCAG CTAGAAAGAA GGCGTCATCA AATTAGTCCT 
CCTCCACCTC CAAGGCCCAT TTCCCCGCCT CATACATATG GTTATATTTC TGGACCAATA 
GTCTCCGATA TGGATACTGA TGTTCCTGAA GAGGAAGGGG ATGATGGGGA AATTGAAGTC 
ACCAAGATGC AAAGTCGCAG ACTTCTTTTA CGTGGCCTGG AGCAGACTCC TGCTTCCAGT 
GTTGGAGACC TAGAAAGTTC TGTGACTGGT TCCATGATCA ATGGATGGGG CTCAGCTTCT 
GAAGAAGACA ATGTTTCCAG TGGACGTTCC AGTGTCAGTT CATCGGATGG ATCATTTTTC 
ACGGATGCGG ATTTTGCACA GGCTGTCGCC GCAGCCGCAG AATATGCTGG GCTTAAAGTA 
GCAAGGCATC AAATGCAGGA AGCAGCAGGA GTGTCGCGTC TTTGCCTCCC AGGTCGAAGA 
CCTTTCCACG CGTCTCACTG TCCAAGACCA ACAAGCCCAG TGTCAACAGA CAGCAACATG 
AGCACCGCTG TCATACAGAA AAGCCGTCCT GCCAAAAAAC AAAAACACCA GCAGGGACAT 
CTTCGGAAAG ATGTCTACAC AGATGATTTG CCACCACCGC CACTGCCACC TCCTGCTATC 
AAATCTCCAA CTGGTCCATC CAAAGCACAA CTAGAAGGAC GGCCTGGCCT TTCACAGAAG 
TACCTTGTTG CAGAACAAAG ACTGGAAAGG CCAGGAGAGA GGAAAGGTGT CAGCTACAGG 
GGCAGGGAAG GTCCTGACAG CAGACAACAA CATGCAGAAG CAAGAACCAA TTCAGGAGAG 
AGAAGAGAGT CTTCGGAACA GCAACTTGAT GGAAAGATGA GAAAAGGGAA AGCTTCAAAG 
TGGGATGGGG CACCTATAGT AGCAAATCAA ATACAAGAGG ATATATTACC CTATTGCAAA 
CCAACATTCC CCACTTCAAA CAACCCTCGT GATCCCAGTT CCTCCAGCTC AATGTCCTCA 
AGAGGATCTG GTGGACGACG GAGGGGAGAA CAAACAACAG GTCGCAGAAA TGCAGCAGAG 
ATGCAGCTCT TAGGAAATTA TGAACGAGGG GTTGATGAAG ATGAAATAGA GGAAACAGAA 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956263.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811717.2 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X8, mRNA.

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CloneID	OXa51069
Clone ID Related Accession (Same CDS sequence)	XM_012956263.3
Accession Version	XM_012956263.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCAG AGTTGGCTCA CTTTTTCCTG CTCTGTGGAA TCATGTGCTT GACTTCAGGG 
TCACGTCTTC GGCAAGAAGA TTTCCCACCG CGAATTGTTG AGCATCCCTC AGACCTAATT 
GTCTCCAAAG GTGAACCAGC AACGCTGAAC TGCAAGGCCG AAGGGAGGCC GACTCCAACC 
ATTGAGTGGT ATAAAGGAGG AGACAAAGTA GAGACAGATA AAGACGATCC TCGCTCTCAC 
CGAATGTTGT TGCCCAGTGG ATCACTATTT TTTTTACGTA TCGTGCATGG ACGCAAAATC 
AGGCCGGATG AAGGGGTCTA TGTTTGTGTT GCAAGGAATT ACCTCGGAGA AGCTGTGAGC 
CACAATGCAT CGCTGGAAGT TGCAATTTTG AGAGACGACT TCAGACAAAA CCCTTCAGAT 
GTAATGGTAG CGGTGGGGGA GCCTGCAGTA ATGGAATGCC AGCCGCCCAG AGGTCATCCT 
GAGCCGACAA TATCCTGGAA AAAAGATGGG ACGCCAATAG ACGATGATAA GGATGAAAGA 
ATAACTATAA GAGGAGGGAA ACTTATGATC ACCTACACCA GGAAAAGCGA TGCTGGAAAA 
TATGTGTGTG TTGGAACCAA TATGGTGGGA GAACGGGAAA GCGAAGTAGC AGAACTTACT 
GTTTTAGAAA GGCCATCATT TGTGAGGAGA CCCAGTAATA TGGCAGTTAC CGTAGATGAT 
AGCGTTGAAT TCAAATGTGA GGCCCGGGGT GATCCTGTCC CTACTGTTCG ATGGAGGAAA 
GATGATGGGG ATCTGCCTAA ATCAAGATAT GAAATCCGTG ATGACCACAC CTTGAAGATA 
AGGAAGGTCA CAGCAGGGGA CATGGGATCC TATACTTGTG TTGCAGAGAA CATGGTTGGA 
AAAGCGGAAG CTTCGGCCGT CCTAACAGTT CAAGTTGTGT CTGAGCCTCC GAATTTTGTT 
GTCAAACCTC GTGACCAAGT TGTTGCTCTG GGACGCACAG TCACATTTCA GTGTGAAGCC 
ACCGGAAACC CCCAGCCAGC CATTTTTTGG CAAAAGGAGG GAAGCCAGAA CCTTCTCTTT 
TCCTACCAAC CTCCACAATC GTCGAGCCGC TTCTCCGTGT CCCAGACTGG TGACCTGACT 
ATAACCACCG TTCAGAAGTC AGACGTGGGC TACTACATCT GCCAGACTCT CAACGTTGCC 
GGGAGCATTA CAACAAAAGC CTTCTTAGAA GTTACCGATG TGGTGGCCGA CCGTCCTCCT 
CCAGTCATTC GTCAGGGACC CGTGAATCAG ACAGTAGCTG TTGATGGCAC CTTAATAATG 
AACTGTATCG CCAGCGGCAT TCCTGTTCCC ACAATCCACT GGAGAAAGGA CGGGATCCTG 
ATACCGACCC AAGACTCTCG CATTAAACAG CAGGACTCAG GAGCTCTGCA GATCCGTTAC 
GCCAAGCTGA GTGATACCGG TCGGTACACC TGCACTGCCT CCACTACTAG CGGGGAAGCA 
AACTGGAGCG CGTACATAGA AGTCCAAGAA TTTGGAGTTC CGGTTCAACC ACCCAGAGCC 
ACTGATCCCA ACCTGATTCC AAGTGCTCCA TCAAAACCAG AAGTCACGGA CGTCAGCAAA 
AATACTGTAA CTTTATCCTG GCAGCCAAAC CTCAACTCAG GTGCTACTCC TACGTCGTAC 
CTCATAGAGG CCTTCAGCCA TGCGTCTGGA AGCAGCTGGC AAACAGTGGC AGAAAATGTC 
AAGACCGATT CCTTTGCAGT TAAAGCACTG AAGCCCAATG CAATCTACCT CTTCCTCGTG 
AGAGCAGTAA ACGCATACGG ATTAAGTGAT CCAAGCCAAA TATCCGATCC TGTAAAAACA 
CAAGATATTC CTCCTACAAG TCAAGGAGTG GATCACAAAC AGGTGCAGAG AGAACTGGGC 
GATGTTGTGC TACACCTTCA TAACCCAACA ATACTTTCCT CTTCCTCCAT CGAAGTACAT 
TGGACTGTGG ACCAGCAATC ACAGTACATC CAAGGATACA AGATCCTCTA CAGGCCAAGC 
GCAGAGAATC GAGGCGAATC TGCATGGTCC ATATTTGAAG TCAGGACTCC GGCCAAAAAC 
AGCGCAGTTA TTCCTGAACT GAGAAAAGGC GTTAACTACG AGATCAAAGC CCGCCCCTTT 
TTTAATGAGT TTCAAGGAGC TGATAGTGAA GTGCGGTTCG CTAAGACAAT GGAAGAAGCA 
CCCAGTGCTC CCCCGCAGAG CGTTACTGTA ACCAAGAATG ATGGGAATGG AACTGCTATT 
GTTGTGGCAT GGCAGCCTCC CCCTGAAGAT ACTCAGAATG GGATGGTCCA GGAATACAAG 
GTTTGGTGCC TGGGGAATGA GAGTCGCTAT CACATAAACA AGACTGTCGA TGGCTCTACC 
CTTTCCGTGG TCATCCCGTC CTTAGTCCCT GGTATCAAAT ACAGCGTGGA GGTGGCAGCC 
AGCACGGGCG CAGGATCCGG TGTGAAAAGT GAACCCAACT ACATCCAGTT AGATTCCTTT 
GGAAACCCGG TGCCTCCGGG GGACCAAGTC AGCCTGGCCC AGCAGATATC CGATGTGGTG 
AAGCAGCCGG CATTCATAGC AGGGATTGGT GCTGCCTGCT GGATAATCCT CATGGTATTC 
AGTATCTGGT TGTACCGGCA CAGAAAGAAA CGCAATGGGC TCACCAGTTC CTATGCTGGA 
ATACGGAAAG TCCCGTCTTT TACCTTCACA CCAACAGTGG CGTACCAGCG AGGAGGGGAA 
GCTGTAAGCA GCGCAGGCCG GCCTGGGCTC TTAAATATTT CTGAGACTGC TAATCAGCCG 
TGGCTCGCAG ATACCTGGCC AAACACTGGA AACAATCACA ACGAATGCCC CATCAACTGC 
TGCACCTCCG GGAATGGCAA CAGCGACAGC AACCTCGCCA CTTACAGCCG CCCAGCTGAC 
TGCATAGCGA ATTACAACAA CCAGTTGGAC AGCAAGCAGA CAAACCTGAT GGTGCCCGAC 
TCGGCTGTTT ATGGGGATGT AGACTTAAGC AACAAGATCA ATGAGATGAA AACCTTCAAC 
AGCCCCAATC TGAAAGATGG GAGGTTTGTC AATCCTGCCG GCCAGCCCAC CCCTTATGCC 
ACCACTCAGC TCATCCAGTC CAACATAAGC AACAACATCA ACACGTCTGC CGGTGATGTG 
GATGAAAAAC ACTGGAAACC CCCGTGCCAA CAGAAACCGG ATGTTACCCC AATGCAGTAC 
AACATCATGG AGCAAAACAA GATCAACAAA GATTACCGAG CTTCGGACAC CATTCCTTCC 
ACCATTCCCT ACAACCAGTC CCATGATCAG AATGCCGGAG GGTCATACAA CAGCTCAGAC 
CGGGGAAGTA GCACATCTGG GAGCCAAGGT CATAAGAAGA GTACTCGTAC CCCGAAAGTA 
TCAAAGCAGG CTGGCATGAA CTGGGCAGAT CTTTTGCCAC CACCACCTGC TCACCCCCCA 
CCACATGGTG CCACTGAAGA CTACACTTTG TCAGCAGATG AAAGCTATGA CAGAGAGTTA 
CCTTGCCCAA TACCACCTAC AAGAATGTAT CTTCAGCAAG ACGAACTGGA AGAGGAAGAT 
GAAAGGGGTC CCACACCTCC TATTAGAGGA GCAGCTTCTT CTCCGGCTGC TGTGTCCTAC 
AGCCATCAGT CCACTGCCAC TCTTACCCCA TCACCTCAGG ACGAACTCCA GCCAATGCTT 
CAGGACTGTC CCGAGGAGAT CGGTCATACT CAGCATCAGC TAGAAAGAAG GCGTCATCAA 
ATTAGTCCTC CTCCACCTCC AAGGCCCATT TCCCCGCCTC ATACATATGG TTATATTTCT 
GGACCAATAG TCTCCGATAT GGATACTGAT GTTCCTGAAG AGGAAGGGGA TGATGGGGAA 
ATTGAAGTCA CCAAGATGCA AAGTCGCAGA CTTCTTTTAC GTGGCCTGGA GCAGACTCCT 
GCTTCCAGTG TTGGAGACCT AGAAAGTTCT GTGACTGGTT CCATGATCAA TGGATGGGGC 
TCAGCTTCTG AAGAAGACAA TGTTTCCAGT GGACGTTCCA GTGTCAGTTC ATCGGATGGA 
TCATTTTTCA CGGATGCGGA TTTTGCACAG GCTGTCGCCG CAGCCGCAGA ATATGCTGGG 
CTTAAAGTAG CAAGGCATCA AATGCAGGAA GCAGCAGGAG TGTCGCGTCT TTGCCTCCCA 
GGTCGAAGAC CTTTCCACGC GTCTCACTGT CCAAGACCAA CAAGCCCAGT GTCAACAGAC 
AGCAACATGA GCACCGCTGT CATACAGAAA AGCCGTCCTG CCAAAAAACA AAAACACCAG 
CAGGGACATC TTCGGAAAGA TGTCTACACA GATGATTTGC CACCACCGCC ACTGCCACCT 
CCTGCTATCA AATCTCCAAC TGGTCCATCC AAAGCACAAC TAGAAGGACG GCCTGGCCTT 
TCACAGAAGT ACCTTGTTGC AGAACAAAGA CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC 
AGCTACAGGG GCAGGGAAGG TCCTGACAGC AGACAACAAC ATGCAGAAGC AAGAACCAAT 
TCAGGAGAGA GAAGAGAGTC TTCGGAACAG CAACTTGATG GAAAGATGAG AAAAGGGAAA 
GCTTCAAAGT GGGATGGGGC ACCTATAGTA GCAAATCAAA TACAAGAGGA TATATTACCC 
TATTGCAAAC CAACATTCCC CACTTCAAAC AACCCTCGTG ATCCCAGTTC CTCCAGCTCA 
ATGTCCTCAA GAGGATCTGG TGGACGACGG AGGGGAGAAC AAACAACAGG TCGCAGAAAT 
GCAGCAGAGA TGCAGCTCTT AGGAAATTAT GAACGAGGGG TTGATGAAGA TGAAATAGAG 
GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811717.2
CDS	651..5525
Translation	MLSELAHFFLLCGIMCLTSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISETANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956263.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956263.3

ORF Insert Sequence:

ATGCTCTCAG AGTTGGCTCA CTTTTTCCTG CTCTGTGGAA TCATGTGCTT GACTTCAGGG 
TCACGTCTTC GGCAAGAAGA TTTCCCACCG CGAATTGTTG AGCATCCCTC AGACCTAATT 
GTCTCCAAAG GTGAACCAGC AACGCTGAAC TGCAAGGCCG AAGGGAGGCC GACTCCAACC 
ATTGAGTGGT ATAAAGGAGG AGACAAAGTA GAGACAGATA AAGACGATCC TCGCTCTCAC 
CGAATGTTGT TGCCCAGTGG ATCACTATTT TTTTTACGTA TCGTGCATGG ACGCAAAATC 
AGGCCGGATG AAGGGGTCTA TGTTTGTGTT GCAAGGAATT ACCTCGGAGA AGCTGTGAGC 
CACAATGCAT CGCTGGAAGT TGCAATTTTG AGAGACGACT TCAGACAAAA CCCTTCAGAT 
GTAATGGTAG CGGTGGGGGA GCCTGCAGTA ATGGAATGCC AGCCGCCCAG AGGTCATCCT 
GAGCCGACAA TATCCTGGAA AAAAGATGGG ACGCCAATAG ACGATGATAA GGATGAAAGA 
ATAACTATAA GAGGAGGGAA ACTTATGATC ACCTACACCA GGAAAAGCGA TGCTGGAAAA 
TATGTGTGTG TTGGAACCAA TATGGTGGGA GAACGGGAAA GCGAAGTAGC AGAACTTACT 
GTTTTAGAAA GGCCATCATT TGTGAGGAGA CCCAGTAATA TGGCAGTTAC CGTAGATGAT 
AGCGTTGAAT TCAAATGTGA GGCCCGGGGT GATCCTGTCC CTACTGTTCG ATGGAGGAAA 
GATGATGGGG ATCTGCCTAA ATCAAGATAT GAAATCCGTG ATGACCACAC CTTGAAGATA 
AGGAAGGTCA CAGCAGGGGA CATGGGATCC TATACTTGTG TTGCAGAGAA CATGGTTGGA 
AAAGCGGAAG CTTCGGCCGT CCTAACAGTT CAAGTTGTGT CTGAGCCTCC GAATTTTGTT 
GTCAAACCTC GTGACCAAGT TGTTGCTCTG GGACGCACAG TCACATTTCA GTGTGAAGCC 
ACCGGAAACC CCCAGCCAGC CATTTTTTGG CAAAAGGAGG GAAGCCAGAA CCTTCTCTTT 
TCCTACCAAC CTCCACAATC GTCGAGCCGC TTCTCCGTGT CCCAGACTGG TGACCTGACT 
ATAACCACCG TTCAGAAGTC AGACGTGGGC TACTACATCT GCCAGACTCT CAACGTTGCC 
GGGAGCATTA CAACAAAAGC CTTCTTAGAA GTTACCGATG TGGTGGCCGA CCGTCCTCCT 
CCAGTCATTC GTCAGGGACC CGTGAATCAG ACAGTAGCTG TTGATGGCAC CTTAATAATG 
AACTGTATCG CCAGCGGCAT TCCTGTTCCC ACAATCCACT GGAGAAAGGA CGGGATCCTG 
ATACCGACCC AAGACTCTCG CATTAAACAG CAGGACTCAG GAGCTCTGCA GATCCGTTAC 
GCCAAGCTGA GTGATACCGG TCGGTACACC TGCACTGCCT CCACTACTAG CGGGGAAGCA 
AACTGGAGCG CGTACATAGA AGTCCAAGAA TTTGGAGTTC CGGTTCAACC ACCCAGAGCC 
ACTGATCCCA ACCTGATTCC AAGTGCTCCA TCAAAACCAG AAGTCACGGA CGTCAGCAAA 
AATACTGTAA CTTTATCCTG GCAGCCAAAC CTCAACTCAG GTGCTACTCC TACGTCGTAC 
CTCATAGAGG CCTTCAGCCA TGCGTCTGGA AGCAGCTGGC AAACAGTGGC AGAAAATGTC 
AAGACCGATT CCTTTGCAGT TAAAGCACTG AAGCCCAATG CAATCTACCT CTTCCTCGTG 
AGAGCAGTAA ACGCATACGG ATTAAGTGAT CCAAGCCAAA TATCCGATCC TGTAAAAACA 
CAAGATATTC CTCCTACAAG TCAAGGAGTG GATCACAAAC AGGTGCAGAG AGAACTGGGC 
GATGTTGTGC TACACCTTCA TAACCCAACA ATACTTTCCT CTTCCTCCAT CGAAGTACAT 
TGGACTGTGG ACCAGCAATC ACAGTACATC CAAGGATACA AGATCCTCTA CAGGCCAAGC 
GCAGAGAATC GAGGCGAATC TGCATGGTCC ATATTTGAAG TCAGGACTCC GGCCAAAAAC 
AGCGCAGTTA TTCCTGAACT GAGAAAAGGC GTTAACTACG AGATCAAAGC CCGCCCCTTT 
TTTAATGAGT TTCAAGGAGC TGATAGTGAA GTGCGGTTCG CTAAGACAAT GGAAGAAGCA 
CCCAGTGCTC CCCCGCAGAG CGTTACTGTA ACCAAGAATG ATGGGAATGG AACTGCTATT 
GTTGTGGCAT GGCAGCCTCC CCCTGAAGAT ACTCAGAATG GGATGGTCCA GGAATACAAG 
GTTTGGTGCC TGGGGAATGA GAGTCGCTAT CACATAAACA AGACTGTCGA TGGCTCTACC 
CTTTCCGTGG TCATCCCGTC CTTAGTCCCT GGTATCAAAT ACAGCGTGGA GGTGGCAGCC 
AGCACGGGCG CAGGATCCGG TGTGAAAAGT GAACCCAACT ACATCCAGTT AGATTCCTTT 
GGAAACCCGG TGCCTCCGGG GGACCAAGTC AGCCTGGCCC AGCAGATATC CGATGTGGTG 
AAGCAGCCGG CATTCATAGC AGGGATTGGT GCTGCCTGCT GGATAATCCT CATGGTATTC 
AGTATCTGGT TGTACCGGCA CAGAAAGAAA CGCAATGGGC TCACCAGTTC CTATGCTGGA 
ATACGGAAAG TCCCGTCTTT TACCTTCACA CCAACAGTGG CGTACCAGCG AGGAGGGGAA 
GCTGTAAGCA GCGCAGGCCG GCCTGGGCTC TTAAATATTT CTGAGACTGC TAATCAGCCG 
TGGCTCGCAG ATACCTGGCC AAACACTGGA AACAATCACA ACGAATGCCC CATCAACTGC 
TGCACCTCCG GGAATGGCAA CAGCGACAGC AACCTCGCCA CTTACAGCCG CCCAGCTGAC 
TGCATAGCGA ATTACAACAA CCAGTTGGAC AGCAAGCAGA CAAACCTGAT GGTGCCCGAC 
TCGGCTGTTT ATGGGGATGT AGACTTAAGC AACAAGATCA ATGAGATGAA AACCTTCAAC 
AGCCCCAATC TGAAAGATGG GAGGTTTGTC AATCCTGCCG GCCAGCCCAC CCCTTATGCC 
ACCACTCAGC TCATCCAGTC CAACATAAGC AACAACATCA ACACGTCTGC CGGTGATGTG 
GATGAAAAAC ACTGGAAACC CCCGTGCCAA CAGAAACCGG ATGTTACCCC AATGCAGTAC 
AACATCATGG AGCAAAACAA GATCAACAAA GATTACCGAG CTTCGGACAC CATTCCTTCC 
ACCATTCCCT ACAACCAGTC CCATGATCAG AATGCCGGAG GGTCATACAA CAGCTCAGAC 
CGGGGAAGTA GCACATCTGG GAGCCAAGGT CATAAGAAGA GTACTCGTAC CCCGAAAGTA 
TCAAAGCAGG CTGGCATGAA CTGGGCAGAT CTTTTGCCAC CACCACCTGC TCACCCCCCA 
CCACATGGTG CCACTGAAGA CTACACTTTG TCAGCAGATG AAAGCTATGA CAGAGAGTTA 
CCTTGCCCAA TACCACCTAC AAGAATGTAT CTTCAGCAAG ACGAACTGGA AGAGGAAGAT 
GAAAGGGGTC CCACACCTCC TATTAGAGGA GCAGCTTCTT CTCCGGCTGC TGTGTCCTAC 
AGCCATCAGT CCACTGCCAC TCTTACCCCA TCACCTCAGG ACGAACTCCA GCCAATGCTT 
CAGGACTGTC CCGAGGAGAT CGGTCATACT CAGCATCAGC TAGAAAGAAG GCGTCATCAA 
ATTAGTCCTC CTCCACCTCC AAGGCCCATT TCCCCGCCTC ATACATATGG TTATATTTCT 
GGACCAATAG TCTCCGATAT GGATACTGAT GTTCCTGAAG AGGAAGGGGA TGATGGGGAA 
ATTGAAGTCA CCAAGATGCA AAGTCGCAGA CTTCTTTTAC GTGGCCTGGA GCAGACTCCT 
GCTTCCAGTG TTGGAGACCT AGAAAGTTCT GTGACTGGTT CCATGATCAA TGGATGGGGC 
TCAGCTTCTG AAGAAGACAA TGTTTCCAGT GGACGTTCCA GTGTCAGTTC ATCGGATGGA 
TCATTTTTCA CGGATGCGGA TTTTGCACAG GCTGTCGCCG CAGCCGCAGA ATATGCTGGG 
CTTAAAGTAG CAAGGCATCA AATGCAGGAA GCAGCAGGAG TGTCGCGTCT TTGCCTCCCA 
GGTCGAAGAC CTTTCCACGC GTCTCACTGT CCAAGACCAA CAAGCCCAGT GTCAACAGAC 
AGCAACATGA GCACCGCTGT CATACAGAAA AGCCGTCCTG CCAAAAAACA AAAACACCAG 
CAGGGACATC TTCGGAAAGA TGTCTACACA GATGATTTGC CACCACCGCC ACTGCCACCT 
CCTGCTATCA AATCTCCAAC TGGTCCATCC AAAGCACAAC TAGAAGGACG GCCTGGCCTT 
TCACAGAAGT ACCTTGTTGC AGAACAAAGA CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC 
AGCTACAGGG GCAGGGAAGG TCCTGACAGC AGACAACAAC ATGCAGAAGC AAGAACCAAT 
TCAGGAGAGA GAAGAGAGTC TTCGGAACAG CAACTTGATG GAAAGATGAG AAAAGGGAAA 
GCTTCAAAGT GGGATGGGGC ACCTATAGTA GCAAATCAAA TACAAGAGGA TATATTACCC 
TATTGCAAAC CAACATTCCC CACTTCAAAC AACCCTCGTG ATCCCAGTTC CTCCAGCTCA 
ATGTCCTCAA GAGGATCTGG TGGACGACGG AGGGGAGAAC AAACAACAGG TCGCAGAAAT 
GCAGCAGAGA TGCAGCTCTT AGGAAATTAT GAACGAGGGG TTGATGAAGA TGAAATAGAG 
GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( NM_001102701.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001096171.1 Xenopus tropicalis roundabout guidance receptor 1 (robo1), mRNA.

Price & Availability↑

CloneID	OXa23658
Clone ID Related Accession (Same CDS sequence)	NM_001102701.1
Accession Version	NM_001102701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC136019.1. ##Evidence-Data-START## Transcript exon combination :: BC136019.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028309, SAMD00028310 [ECO:0000348] ##Evidence-Data-END##

ATGAAACTTC CCAACACTGC TGCTATGTTT CTGGTGGCAC TGTCTGGTCT ACTTTTCCAG 
CCATTGGTGG GACAACTGAT TCCAGACTCC GATGATCTTG ACCGGGGGTT GGACAGCGGG 
ACGGCACTTC CTACATCTGA TCATGATGAC AATTCGCTGG TCTATACAGG GTCACGTCTT 
CGGCAAGAAG ATTTCCCACC GCGAATTGTT GAGCATCCCT CAGACCTAAT TGTCTCCAAA 
GGTGAACCAG CAACGCTGAA CTGCAAGGCC GAAGGGAGGC CGACTCCAAC CATTGAGTGG 
TATAAAGGAG GAGACAAAGT AGAGACAGAT AAAGACGATC CTCGCTCTCA CCGAATGTTG 
TTGCCCAGTG GATCACTATT TTTTTTACGT ATCGTGCATG GACGCAAAAT CAGGCCGGAT 
GAAGGGGTCT ATGTTTGTGT TGCAAGGAAT TACCTCGGAG AAGCTGTGAG CCACAATGCA 
TCGCTGGAAG TTGCAATTTT GAGAGACGAC TTCAGACAAA ACCCTTCAGA TGTAATGGTA 
GCGGTGGGGG AGCCTGCAGT AATGGAATGC CAGCCGCCCA GAGGTCATCC TGAGCCGACA 
ATATCCTGGA AAAAAGATGG GACGCCAATA GACGATGATA AGGATGAAAG AATAACTATA 
AGAGGAGGGA AACTTATGAT CACCTACACC AGGAAAAGCG ATGCTGGAAA ATATGTGTGT 
GTTGGAACCA ATATGGTGGG AGAACGGGAA AGCGAAGTAG CAGAACTTAC TGTTTTAGAA 
AGGCCATCAT TTGTGAGGAG ACCCAGTAAT ATGGCAGTTA CCGTAGATGA TAGCGTTGAA 
TTCAAATGTG AGGCCCGGGG TGATCCTGTC CCTACTGTTC GATGGAGGAA AGATGATGGG 
GATCTGCCTA AATCAAGATA TGAAATCCGT GATGACCACA CCTTGAAGAT AAGGAAGGTC 
ACAGCAGGGG ACATGGGATC CTATACTTGT GTTGCAGAGA ACATGGTCGG AAAAGCGGAA 
GCTTCGGCCG TCCTAACAGT TCAAGAGCCT CCGAATTTTG TTGTCAAACC TCGTGACCAA 
GTTGTTGCTC TGGGACGCAC AGTCACATTT CAGTGTGAAG CCACCGGAAA CCCCCAGCCA 
GCCATTTTTT GGCAAAAGGA GGGAAGCCAG AACCTTCTCT TTTCCTACCA ACCTCCACAA 
TCGTCGAGCC GCTTCTCCGT GTCCCAGACT GGTGACCTGA CTATAACCAC CGTTCAGAAG 
TCAGACGTGG GCTACTACAT CTGCCAGACT CTCAACGTTG CCGGGAGCAT TACAACAAAA 
GCCTTCTTAG AAGTTACCGA TGTGGTGGCC GACCGTCCTC CTCCAGTCAT TCGTCAGGGA 
CCCGTGAATC AGACAGTAGC TGTCGATGGC ACCTTAATAA TGAACTGTAT CGCCAGCGGC 
ATTCCTGTTC CCACAATCCA CTGGAGAAAG GACGGGATCC TGATACCGAC CCAAGACTCT 
CGCATTAAAC AGCAGGACTC AGGAGCTCTG CAGATCCGTT ACGCCAAGCT GAGTGATACC 
GGTCGGTACA CCTGCACTGC CTCCACTACT AGCGGGGAAG CAAACTGGAG CGCGTACATA 
GAAGTCCAAG AATTTGGAGT TCCGGTTCAA CCACCCAGAG CCACTGATCC CAACCTGATT 
CCAAGTGCTC CATCAAAACC AGAAGTCACG GACGTCAGCA AAAATACTGT AACTTTATCC 
TGGCAGCCAA ACCTCAACTC AGGTGCTACT CCTACGTCGT ACCTCATAGA GGCCTTCAGC 
CATGCGTCTG GAAGCAGCTG GCAAACAGTG GCAGAAAATG TCAAGACCGA TTCCTTTGCA 
GTTAAAGCAC TGAAGCCCAA TGCAATCTAC CTCTTCCTCG TGAGAGCAGT AAACGCATAC 
GGATTAAGTG ATCCAAGCCA AATATCCGAT CCTGTAAAAA CACAAGATAT TCCTCCTACA 
AGTCAAGGAG TGGATCACAA ACAGGTGCAG AGAGAACTGG GCGATGTTGT GCTACACCTT 
CATAACCCAA CAATACTTTC CTCTTCCTCC ATCGAAGTAC ATTGGACTGT GGACCAGCAA 
TCACAGTACA TCCAAGGATA CAAGATCCTC TACAGGCCAA GCGCAGAGAA TCGAGGCGAA 
TCTGCATGGT CCATATTTGA AGTCAGGACT CCGGCCAAAA ACAGCGCAGT TATTCCTGAA 
CTGAGAAAAG GCGTTAACTA CGAGATCAAA GCCCGCCCCT TTTTTAATGA GTTTCAAGGA 
GCTGATAGTG AAGTGCGGTT CGCTAAGACA ATGGAAGAAG CACCCAGTGC TCCCCCGCAG 
AGCGTTACTG TAACCAAGAA TGATGGGAAT GGAACTGCTA TTGTTGTGGC ATGGCAGCCT 
CCCCCTGAAG ATACTCAGAA TGGGATGGTC CAGGAATACA AGGTTTGGTG CCTGGGGAAT 
GAGAGTCGCT ATCACATAAA CAAGACTGTC GATGGCTCTA CCCTTTCCGT GGTCATCCCA 
TCCTTAGTCC CTGTTATCAA ATACAGCGTG GAGGTGGCAG CCAGCACGGG CGCAGGATCC 
GGTGTGAAAA GTGAACCCAA CTACATCCAG TTAGATTCCT TTGGAAACCC GGTGCCTCCG 
GGGGACCAAG TCAGCCTGGC CCAGCAGATA TCCGATGTGG TGAAGCAGCC GGCATTCATA 
GCAGGGATTG GTGCTGCCTG CTGGATAATC CTCATGGTAT TCAGTATCTG GTTGTACCGG 
CACAGAAAGA AACGCAATGG GCTCACCAGT TCCTATGCTG GAATACGGAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT GGCGTACCAG CGAGGAGGGG AAGCTGTAAG CAGCGCAGGC 
CGGCCTGGGC TCTTAAATAT TTCTGAGACT GCTAATCAGC CGTGGCTCGC AGATACCTGG 
CCAAACACTG GAAACAATCA CAACGAATGC CCCATCAACT GCTGCACCTC CGGGAATGGC 
AACAGCGACA GCAACCTCGC CACTTACAGC CGCCCAGCTG ACTGCATAGC GAATTACAAC 
AACCAGTTGG ACAGCAAGCA GACAAACCTG ATGGTGCCTG ACTCGGCTGT TTATGGGGAT 
GTAGACTTAA GCAACAAGAT CAATGAGATG AAAACCTTCA ACAGCCCCAA TCTGAAAGAT 
GGGAGGTTTG TCAATCCTGC CGGCCAGCCC ACCCCTTATG CCACCACTCA GCTCATCCAG 
TCCAACATAA GCAACAACAT CAACACGTCT GCCGGTGATG TGGATGAAAA ACACTGGAAA 
CCCCCGTGCC AACAGAAACC GGATGTTACC CCAATGCAGT ACAACATCAT GGAGCAAAAC 
AAGATCAACA AAGATTACCG AGCTTCGGAC ACCATTCCTT CCACCATTCC CTACAACCAG 
TCCCATGATC AGAATGCCGG AGGGTCATAC AACAGCTCAG ACCGGGGAAG TAGCACATCT 
GGGAGCCAAG GTCATAAGAA GAGTACTCGT ACCCCGAAAG TATCAAAGCA GGCTGGCATG 
AACTGGGCAG ATCTTTTGCC ACCACCACCT GCTCACCCCC CACCACATGG TGCCACTGAA 
GACTACACTT TGTCAGCAGA TGAAAGCTAT GACAGAGAGT TACCTTGCCC AATACCACCT 
ACAAGAATGT ATCTTCAGCA AGACGAACTG GAAGAGGAAG ATGAAAGGGG TCCCACACCT 
CCTATTAGAG GAGCAGCTTC TTCTCCGGCT GCTGTGTCCT ACAGCCATCA GTCCACTGCC 
ACTCTTACCC CATCACCTCA GGACGAACTC CAGCCAATGC TTCAGGACTG TCCCGAGGAG 
ATCGGTCATA CTCAGCATCA GCTAGAAAGA AGGCGTCATC AAATTAGTCC TCCTCCACCT 
CCAAGGCCCA TTTCCCCGCC TCATACATAT GGTTATATTT CTGGACCAAT AGTCTCCGAT 
ATGGATACTG ATGTTCCTGA AGAGGAAGGG GATGACGGGG AAATTGAAGT CACCAAGATG 
CAAAGTCGCA GACTTCTTTT ACGTGGCCTG GAGCAGACTC CTGCTTCCAG TGTTGGAGAC 
CTAGAAAGTT CTGTGACTGG TTCCATGATC AATGGATGGG GCTCAGCTTC TGAAGAAGAC 
AATGTTTCCA GTGGACGTTC CAGTGTCAGT TCATCGGATG GATCATTTTT CACGGATGCG 
GATTTTGCAC AGGCTGTCGC CGCAGCCGCA GAATATGCTG GGCTTAAAGT AGCAAGGCAT 
CAAATGCAGG AAGCAGCAGG AGGTCGAAGA CCTTTCCACG CGTCTCACTG TCCAAGACCA 
ACAAGCCCAG TGTCAACAGA CAGCAACATG AGCACCGCTG TCATACAGAA AAGCCGTCCT 
GCCAAAAAAC AAAAACACCA GCAGGGACAT CTTCGGAAAG ATGTCTACAC AGATGATTTG 
CCACCACCGC CACTGCCACC TCCTGCTATC AAATCTCCAA CTGGTCCATC CAAAGCACAA 
CTAGAAGGAC GGCCTGGCCT TTCACAGAAG TACCTTGTTG CAGAACAAAG ACTGGAAAGG 
CCAGGAGAGA GGAAAGGTGT CAGCTACAGG GGCAGGGAAG GTCCTGACAG CAGACAACAA 
CATGCAGAAG CAAGAACCAA TTCAGGGGAG AGAAGAGAGT CTTCGGAACA GCAACTTGAT 
GGAAAGATGA GAAAAGGGAA AGCTTCAAAG TGGGATGGGG CACCTATAGT AGCAAATCAA 
ATACAAGAGG ATATATTACC CTATTGCAAA CCAACATTCC CCACTTCAAA CAACCCTCGT 
GATCCCAGTT CCTCCAGCTC AATGTCCTCA AGAGGATCTG GTGGACGACG GAGGGGAGAA 
CAAACAACAG GTCGCAGAAA TGCAGCAGAG ATGCAGCTCT TAGGAAATTA TGAACGAGGG 
GTTGATGAAG ATGAAATAGA GGAAACAGAA AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096171.1
CDS	136..5091
Translation	MKLPNTAAMFLVALSGLLFQPLVGQLIPDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPVIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISETANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	NM_001102701.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102701.1

ORF Insert Sequence:

ATGAAACTTC CCAACACTGC TGCTATGTTT CTGGTGGCAC TGTCTGGTCT ACTTTTCCAG 
CCATTGGTGG GACAACTGAT TCCAGACTCC GATGATCTTG ACCGGGGGTT GGACAGCGGG 
ACGGCACTTC CTACATCTGA TCATGATGAC AATTCGCTGG TCTATACAGG GTCACGTCTT 
CGGCAAGAAG ATTTCCCACC GCGAATTGTT GAGCATCCCT CAGACCTAAT TGTCTCCAAA 
GGTGAACCAG CAACGCTGAA CTGCAAGGCC GAAGGGAGGC CGACTCCAAC CATTGAGTGG 
TATAAAGGAG GAGACAAAGT AGAGACAGAT AAAGACGATC CTCGCTCTCA CCGAATGTTG 
TTGCCCAGTG GATCACTATT TTTTTTACGT ATCGTGCATG GACGCAAAAT CAGGCCGGAT 
GAAGGGGTCT ATGTTTGTGT TGCAAGGAAT TACCTCGGAG AAGCTGTGAG CCACAATGCA 
TCGCTGGAAG TTGCAATTTT GAGAGACGAC TTCAGACAAA ACCCTTCAGA TGTAATGGTA 
GCGGTGGGGG AGCCTGCAGT AATGGAATGC CAGCCGCCCA GAGGTCATCC TGAGCCGACA 
ATATCCTGGA AAAAAGATGG GACGCCAATA GACGATGATA AGGATGAAAG AATAACTATA 
AGAGGAGGGA AACTTATGAT CACCTACACC AGGAAAAGCG ATGCTGGAAA ATATGTGTGT 
GTTGGAACCA ATATGGTGGG AGAACGGGAA AGCGAAGTAG CAGAACTTAC TGTTTTAGAA 
AGGCCATCAT TTGTGAGGAG ACCCAGTAAT ATGGCAGTTA CCGTAGATGA TAGCGTTGAA 
TTCAAATGTG AGGCCCGGGG TGATCCTGTC CCTACTGTTC GATGGAGGAA AGATGATGGG 
GATCTGCCTA AATCAAGATA TGAAATCCGT GATGACCACA CCTTGAAGAT AAGGAAGGTC 
ACAGCAGGGG ACATGGGATC CTATACTTGT GTTGCAGAGA ACATGGTCGG AAAAGCGGAA 
GCTTCGGCCG TCCTAACAGT TCAAGAGCCT CCGAATTTTG TTGTCAAACC TCGTGACCAA 
GTTGTTGCTC TGGGACGCAC AGTCACATTT CAGTGTGAAG CCACCGGAAA CCCCCAGCCA 
GCCATTTTTT GGCAAAAGGA GGGAAGCCAG AACCTTCTCT TTTCCTACCA ACCTCCACAA 
TCGTCGAGCC GCTTCTCCGT GTCCCAGACT GGTGACCTGA CTATAACCAC CGTTCAGAAG 
TCAGACGTGG GCTACTACAT CTGCCAGACT CTCAACGTTG CCGGGAGCAT TACAACAAAA 
GCCTTCTTAG AAGTTACCGA TGTGGTGGCC GACCGTCCTC CTCCAGTCAT TCGTCAGGGA 
CCCGTGAATC AGACAGTAGC TGTCGATGGC ACCTTAATAA TGAACTGTAT CGCCAGCGGC 
ATTCCTGTTC CCACAATCCA CTGGAGAAAG GACGGGATCC TGATACCGAC CCAAGACTCT 
CGCATTAAAC AGCAGGACTC AGGAGCTCTG CAGATCCGTT ACGCCAAGCT GAGTGATACC 
GGTCGGTACA CCTGCACTGC CTCCACTACT AGCGGGGAAG CAAACTGGAG CGCGTACATA 
GAAGTCCAAG AATTTGGAGT TCCGGTTCAA CCACCCAGAG CCACTGATCC CAACCTGATT 
CCAAGTGCTC CATCAAAACC AGAAGTCACG GACGTCAGCA AAAATACTGT AACTTTATCC 
TGGCAGCCAA ACCTCAACTC AGGTGCTACT CCTACGTCGT ACCTCATAGA GGCCTTCAGC 
CATGCGTCTG GAAGCAGCTG GCAAACAGTG GCAGAAAATG TCAAGACCGA TTCCTTTGCA 
GTTAAAGCAC TGAAGCCCAA TGCAATCTAC CTCTTCCTCG TGAGAGCAGT AAACGCATAC 
GGATTAAGTG ATCCAAGCCA AATATCCGAT CCTGTAAAAA CACAAGATAT TCCTCCTACA 
AGTCAAGGAG TGGATCACAA ACAGGTGCAG AGAGAACTGG GCGATGTTGT GCTACACCTT 
CATAACCCAA CAATACTTTC CTCTTCCTCC ATCGAAGTAC ATTGGACTGT GGACCAGCAA 
TCACAGTACA TCCAAGGATA CAAGATCCTC TACAGGCCAA GCGCAGAGAA TCGAGGCGAA 
TCTGCATGGT CCATATTTGA AGTCAGGACT CCGGCCAAAA ACAGCGCAGT TATTCCTGAA 
CTGAGAAAAG GCGTTAACTA CGAGATCAAA GCCCGCCCCT TTTTTAATGA GTTTCAAGGA 
GCTGATAGTG AAGTGCGGTT CGCTAAGACA ATGGAAGAAG CACCCAGTGC TCCCCCGCAG 
AGCGTTACTG TAACCAAGAA TGATGGGAAT GGAACTGCTA TTGTTGTGGC ATGGCAGCCT 
CCCCCTGAAG ATACTCAGAA TGGGATGGTC CAGGAATACA AGGTTTGGTG CCTGGGGAAT 
GAGAGTCGCT ATCACATAAA CAAGACTGTC GATGGCTCTA CCCTTTCCGT GGTCATCCCA 
TCCTTAGTCC CTGTTATCAA ATACAGCGTG GAGGTGGCAG CCAGCACGGG CGCAGGATCC 
GGTGTGAAAA GTGAACCCAA CTACATCCAG TTAGATTCCT TTGGAAACCC GGTGCCTCCG 
GGGGACCAAG TCAGCCTGGC CCAGCAGATA TCCGATGTGG TGAAGCAGCC GGCATTCATA 
GCAGGGATTG GTGCTGCCTG CTGGATAATC CTCATGGTAT TCAGTATCTG GTTGTACCGG 
CACAGAAAGA AACGCAATGG GCTCACCAGT TCCTATGCTG GAATACGGAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT GGCGTACCAG CGAGGAGGGG AAGCTGTAAG CAGCGCAGGC 
CGGCCTGGGC TCTTAAATAT TTCTGAGACT GCTAATCAGC CGTGGCTCGC AGATACCTGG 
CCAAACACTG GAAACAATCA CAACGAATGC CCCATCAACT GCTGCACCTC CGGGAATGGC 
AACAGCGACA GCAACCTCGC CACTTACAGC CGCCCAGCTG ACTGCATAGC GAATTACAAC 
AACCAGTTGG ACAGCAAGCA GACAAACCTG ATGGTGCCTG ACTCGGCTGT TTATGGGGAT 
GTAGACTTAA GCAACAAGAT CAATGAGATG AAAACCTTCA ACAGCCCCAA TCTGAAAGAT 
GGGAGGTTTG TCAATCCTGC CGGCCAGCCC ACCCCTTATG CCACCACTCA GCTCATCCAG 
TCCAACATAA GCAACAACAT CAACACGTCT GCCGGTGATG TGGATGAAAA ACACTGGAAA 
CCCCCGTGCC AACAGAAACC GGATGTTACC CCAATGCAGT ACAACATCAT GGAGCAAAAC 
AAGATCAACA AAGATTACCG AGCTTCGGAC ACCATTCCTT CCACCATTCC CTACAACCAG 
TCCCATGATC AGAATGCCGG AGGGTCATAC AACAGCTCAG ACCGGGGAAG TAGCACATCT 
GGGAGCCAAG GTCATAAGAA GAGTACTCGT ACCCCGAAAG TATCAAAGCA GGCTGGCATG 
AACTGGGCAG ATCTTTTGCC ACCACCACCT GCTCACCCCC CACCACATGG TGCCACTGAA 
GACTACACTT TGTCAGCAGA TGAAAGCTAT GACAGAGAGT TACCTTGCCC AATACCACCT 
ACAAGAATGT ATCTTCAGCA AGACGAACTG GAAGAGGAAG ATGAAAGGGG TCCCACACCT 
CCTATTAGAG GAGCAGCTTC TTCTCCGGCT GCTGTGTCCT ACAGCCATCA GTCCACTGCC 
ACTCTTACCC CATCACCTCA GGACGAACTC CAGCCAATGC TTCAGGACTG TCCCGAGGAG 
ATCGGTCATA CTCAGCATCA GCTAGAAAGA AGGCGTCATC AAATTAGTCC TCCTCCACCT 
CCAAGGCCCA TTTCCCCGCC TCATACATAT GGTTATATTT CTGGACCAAT AGTCTCCGAT 
ATGGATACTG ATGTTCCTGA AGAGGAAGGG GATGACGGGG AAATTGAAGT CACCAAGATG 
CAAAGTCGCA GACTTCTTTT ACGTGGCCTG GAGCAGACTC CTGCTTCCAG TGTTGGAGAC 
CTAGAAAGTT CTGTGACTGG TTCCATGATC AATGGATGGG GCTCAGCTTC TGAAGAAGAC 
AATGTTTCCA GTGGACGTTC CAGTGTCAGT TCATCGGATG GATCATTTTT CACGGATGCG 
GATTTTGCAC AGGCTGTCGC CGCAGCCGCA GAATATGCTG GGCTTAAAGT AGCAAGGCAT 
CAAATGCAGG AAGCAGCAGG AGGTCGAAGA CCTTTCCACG CGTCTCACTG TCCAAGACCA 
ACAAGCCCAG TGTCAACAGA CAGCAACATG AGCACCGCTG TCATACAGAA AAGCCGTCCT 
GCCAAAAAAC AAAAACACCA GCAGGGACAT CTTCGGAAAG ATGTCTACAC AGATGATTTG 
CCACCACCGC CACTGCCACC TCCTGCTATC AAATCTCCAA CTGGTCCATC CAAAGCACAA 
CTAGAAGGAC GGCCTGGCCT TTCACAGAAG TACCTTGTTG CAGAACAAAG ACTGGAAAGG 
CCAGGAGAGA GGAAAGGTGT CAGCTACAGG GGCAGGGAAG GTCCTGACAG CAGACAACAA 
CATGCAGAAG CAAGAACCAA TTCAGGGGAG AGAAGAGAGT CTTCGGAACA GCAACTTGAT 
GGAAAGATGA GAAAAGGGAA AGCTTCAAAG TGGGATGGGG CACCTATAGT AGCAAATCAA 
ATACAAGAGG ATATATTACC CTATTGCAAA CCAACATTCC CCACTTCAAA CAACCCTCGT 
GATCCCAGTT CCTCCAGCTC AATGTCCTCA AGAGGATCTG GTGGACGACG GAGGGGAGAA 
CAAACAACAG GTCGCAGAAA TGCAGCAGAG ATGCAGCTCT TAGGAAATTA TGAACGAGGG 
GTTGATGAAG ATGAAATAGA GGAAACAGAA AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956257.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811711.1 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X7, mRNA.

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CloneID	OXa30328
Clone ID Related Accession (Same CDS sequence)	XM_012956257.2
Accession Version	XM_012956257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTTC CCAACACTGC TGCTATGTTT CTGGTGGCAC TGTCTGGTCT ACTTTTCCAG 
CCATTGGTGG GACAACTGAT TCCAGACTCC GATGATCTTG ACCGGGGGTT GGACAGCGGG 
ACGGCACTTC CTACATCTGA TCATGATGAC AATTCGCTGG TCTATACAGG GTCACGTCTT 
CGGCAAGAAG ATTTCCCACC GCGAATTGTT GAGCATCCCT CAGACCTAAT TGTCTCCAAA 
GGTGAACCAG CAACGCTGAA CTGCAAGGCC GAAGGGAGGC CGACTCCAAC CATTGAGTGG 
TATAAAGGAG GAGACAAAGT AGAGACAGAT AAAGACGATC CTCGCTCTCA CCGAATGTTG 
TTGCCCAGTG GATCACTATT TTTTTTACGT ATCGTGCATG GACGCAAAAT CAGGCCGGAT 
GAAGGGGTCT ATGTTTGTGT TGCAAGGAAT TACCTCGGAG AAGCTGTGAG CCACAATGCA 
TCGCTGGAAG TTGCAATTTT GAGAGACGAC TTCAGACAAA ACCCTTCAGA TGTAATGGTA 
GCGGTGGGGG AGCCTGCAGT AATGGAATGC CAGCCGCCCA GAGGTCATCC TGAGCCGACA 
ATATCCTGGA AAAAAGATGG GACGCCAATA GACGATGATA AGGATGAAAG AATAACTATA 
AGAGGAGGGA AACTTATGAT CACCTACACC AGGAAAAGCG ATGCTGGAAA ATATGTGTGT 
GTTGGAACCA ATATGGTGGG AGAACGGGAA AGCGAAGTAG CAGAACTTAC TGTTTTAGAA 
AGGCCATCAT TTGTGAGGAG ACCCAGTAAT ATGGCAGTTA CCGTAGATGA TAGCGTTGAA 
TTCAAATGTG AGGCCCGGGG TGATCCTGTC CCTACTGTTC GATGGAGGAA AGATGATGGG 
GATCTGCCTA AATCAAGATA TGAAATCCGT GATGACCACA CCTTGAAGAT AAGGAAGGTC 
ACAGCAGGGG ACATGGGATC CTATACTTGT GTTGCAGAGA ACATGGTCGG AAAAGCGGAA 
GCTTCGGCCG TCCTAACAGT TCAAGTTGTG TCTGAGCCTC CGAATTTTGT TGTCAAACCT 
CGTGACCAAG TTGTTGCTCT GGGACGCACA GTCACATTTC AGTGTGAAGC CACCGGAAAC 
CCCCAGCCAG CCATTTTTTG GCAAAAGGAG GGAAGCCAGA ACCTTCTCTT TTCCTACCAA 
CCTCCACAAT CGTCGAGCCG CTTCTCCGTG TCCCAGACTG GTGACCTGAC TATAACCACC 
GTTCAGAAGT CAGACGTGGG CTACTACATC TGCCAGACTC TCAACGTTGC CGGGAGCATT 
ACAACAAAAG CCTTCTTAGA AGTTACCGAT GTGGTGGCCG ACCGTCCTCC TCCAGTCATT 
CGTCAGGGAC CCGTGAATCA GACAGTAGCT GTTGATGGCA CCTTAATAAT GAACTGTATC 
GCCAGCGGCA TTCCTGTTCC CACAATCCAC TGGAGAAAGG ACGGGATCCT GATACCGACC 
CAAGACTCTC GCATTAAACA GCAGGACTCA GGAGCTCTGC AGATCCGTTA CGCCAAGCTG 
AGTGATACCG GTCGGTACAC CTGCACTGCC TCCACTACTA GCGGGGAAGC AAACTGGAGC 
GCGTACATAG AAGTCCAAGA ATTTGGAGTT CCGGTTCAAC CACCCAGAGC CACTGATCCC 
AACCTGATTC CAAGTGCTCC ATCAAAACCA GAAGTCACGG ACGTCAGCAA AAATACTGTA 
ACTTTATCCT GGCAGCCAAA CCTCAACTCA GGTGCTACTC CTACGTCGTA CCTCATAGAG 
GCCTTCAGCC ATGCGTCTGG AAGCAGCTGG CAAACAGTGG CAGAAAATGT CAAGACCGAT 
TCCTTTGCAG TTAAAGCACT GAAGCCCAAT GCAATCTACC TCTTCCTCGT GAGAGCAGTA 
AACGCATACG GATTAAGTGA TCCAAGCCAA ATATCCGATC CTGTAAAAAC ACAAGATATT 
CCTCCTACAA GTCAAGGAGT GGATCACAAA CAGGTGCAGA GAGAACTGGG CGATGTTGTG 
CTACACCTTC ATAACCCAAC AATACTTTCC TCTTCCTCCA TCGAAGTACA TTGGACTGTG 
GACCAGCAAT CACAGTACAT CCAAGGATAC AAGATCCTCT ACAGGCCAAG CGCAGAGAAT 
CGAGGCGAAT CTGCATGGTC CATATTTGAA GTCAGGACTC CGGCCAAAAA CAGCGCAGTT 
ATTCCTGAAC TGAGAAAAGG CGTTAACTAC GAGATCAAAG CCCGCCCCTT TTTTAATGAG 
TTTCAAGGAG CTGATAGTGA AGTGCGGTTC GCTAAGACAA TGGAAGAAGC ACCCAGTGCT 
CCCCCGCAGA GCGTTACTGT AACCAAGAAT GATGGGAATG GAACTGCTAT TGTTGTGGCA 
TGGCAGCCTC CCCCTGAAGA TACTCAGAAT GGGATGGTCC AGGAATACAA GGTTTGGTGC 
CTGGGGAATG AGAGTCGCTA TCACATAAAC AAGACTGTCG ATGGCTCTAC CCTTTCCGTG 
GTCATCCCGT CCTTAGTCCC TGGTATCAAA TACAGCGTGG AGGTGGCAGC CAGCACGGGC 
GCAGGATCCG GTGTGAAAAG TGAACCCAAC TACATCCAGT TAGATTCCTT TGGAAACCCG 
GTGCCTCCGG GGGACCAAGT CAGCCTGGCC CAGCAGATAT CCGATGTGGT GAAGCAGCCG 
GCATTCATAG CAGGGATTGG TGCTGCCTGC TGGATAATCC TCATGGTATT CAGTATCTGG 
TTGTACCGGC ACAGAAAGAA ACGCAATGGG CTCACCAGTT CCTATGCTGG AATACGGAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTG GCGTACCAGC GAGGAGGGGA AGCTGTAAGC 
AGCGCAGGCC GGCCTGGGCT CTTAAATATT TCTGAGCCTG CTAATCAGCC GTGGCTCGCA 
GATACCTGGC CAAACACTGG AAACAATCAC AACGAATGCC CCATCAACTG CTGCACCTCC 
GGGAACGGCA ACAGCGACAG CAACCTCGCC ACTTACAGCC GCCCAGCTGA CTGCATAGCG 
AATTACAACA ACCAGTTGGA CAGCAAGCAG ACAAACCTGA TGGTGCCCGA CTCGGCTGTT 
TATGGGGATG TAGACTTAAG CAACAAGATC AATGAGATGA AAACCTTCAA CAGCCCCAAT 
CTGAAAGATG GGAGGTTTGT CAATCCTGCC GGCCAGCCCA CCCCTTATGC CACCACTCAG 
CTCATCCAGT CCAACATAAG CAACAACATC AACACGTCTG CCGGTGATGT GGATGAAAAA 
CACTGGAAAC CCCCGTGCCA ACAGAAACCG GATGTTACCC CAATGCAGTA CAACATCATG 
GAGCAAAACA AGATCAACAA AGATTACCGA GCTTCGGACA CCATTCCTTC CACCATTCCC 
TACAACCAGT CCCATGATCA GAATGCCGGA GGGTCATACA ACAGCTCAGA CCGGGGAAGT 
AGCACATCTG GGAGCCAAGG TCATAAGAAG AGTACTCGTA CCCCGAAAGT ATCAAAGCAG 
GCTGGCATGA ACTGGGCAGA TCTTTTGCCA CCACCACCTG CTCACCCCCC ACCACATGGT 
GCCACTGAAG ACTACACTTT GTCAGCAGAT GAAAGCTATG ACAGAGAGTT ACCTTGCCCA 
ATACCACCTA CAAGAATGTA TCTTCAGCAA GACGAACTGG AAGAGGAAGA TGAAAGGGGT 
CCCACACCTC CTATTAGAGG AGCAGCTTCT TCTCCGGCTG CTGTGTCCTA CAGCCATCAG 
TCCACTGCCA CTCTTACCCC ATCACCTCAG GACGAACTCC AGCCAATGCT TCAGGACTGT 
CCCGAGGAGA TCGGTCATAC TCAGCATCAG CTAGAAAGAA GGCGTCATCA AATTAGTCCT 
CCTCCACCTC CAAGGCCCAT TTCCCCGCCT CATACATATG GTTATATTTC TGGACCAATA 
GTCTCCGATA TGGATACTGA TGTTCCTGAA GAGGAAGGGG ATGATGGGGA AATTGAAGTC 
ACCAAGATGC AAAGTCGCAG ACTTCTTTTA CGTGGCCTGG AGCAGACTCC TGCTTCCAGT 
GTTGGAGACC TAGAAAGTTC TGTGACTGGT TCCATGATCA ATGGATGGGG CTCAGCTTCT 
GAAGAAGACA ATGTTTCCAG TGGACGTTCC AGTGTCAGTT CATCGGATGG ATCATTTTTC 
ACGGATGCGG ATTTTGCACA GGCTGTCGCC GCAGCCGCAG AATATGCTGG GCTTAAAGTA 
GCAAGGCATC AAATGCAGGA AGCAGCAGGA GTGTCGCGTC TTTGCCTCCC AGGTCGAAGA 
CCTTTCCACG CGTCTCACTG TCCAAGACCA ACAAGCCCAG TGTCAACAGA CAGCAACATG 
AGCACCGCTG TCATACAGAA AAGCCGTCCT GCCAAAAAAC AAAAACACCA GCAGGGACAT 
CTTCGGAAAG ATGTCTACAC AGATGATTTG CCACCACCGC CACTGCCACC TCCTGCTATC 
AAATCTCCAA CTGGTCCATC CAAAGCACAA CTAGAAGGAC GGCCTGGCCT TTCACAGAAG 
TACCTTGTTG CAGAACAAAG ACTGGAAAGG CCAGGAGAGA GGAAAGGTGT CAGCTACAGG 
GGCAGGGAAG GTCCTGACAG CAGACAACAA CATGCAGAAG CAAGAACCAA TTCAGGGGAG 
AGAAGAGAGT CTTCGGAACA GCAACTTGAT GGAAAGATGA GAAAAGGGAA AGCTTCAAAG 
TGGGATGGGG CACCTATAGT AGCAAATCAA ATACAAGAGG ATATATTACC CTATTGCAAA 
CCAACATTCC CCACTTCAAA CAACCCTCGT GATCCCAGTT CCTCCAGCTC AATGTCCTCA 
AGAGGATCTG GTGGACGACG GAGGGGAGAA CAAACAACAG GTCGCAGAAA TGCAGCAGAG 
ATGCAGCTCT TAGGAAATTA TGAACGAGGG GTTGATGAAG ATGAAATAGA GGAAACAGAA 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811711.1
CDS	166..5151
Translation	MKLPNTAAMFLVALSGLLFQPLVGQLIPDSDDLDRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISEPANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956257.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956257.2

ORF Insert Sequence:

ATGAAACTTC CCAACACTGC TGCTATGTTT CTGGTGGCAC TGTCTGGTCT ACTTTTCCAG 
CCATTGGTGG GACAACTGAT TCCAGACTCC GATGATCTTG ACCGGGGGTT GGACAGCGGG 
ACGGCACTTC CTACATCTGA TCATGATGAC AATTCGCTGG TCTATACAGG GTCACGTCTT 
CGGCAAGAAG ATTTCCCACC GCGAATTGTT GAGCATCCCT CAGACCTAAT TGTCTCCAAA 
GGTGAACCAG CAACGCTGAA CTGCAAGGCC GAAGGGAGGC CGACTCCAAC CATTGAGTGG 
TATAAAGGAG GAGACAAAGT AGAGACAGAT AAAGACGATC CTCGCTCTCA CCGAATGTTG 
TTGCCCAGTG GATCACTATT TTTTTTACGT ATCGTGCATG GACGCAAAAT CAGGCCGGAT 
GAAGGGGTCT ATGTTTGTGT TGCAAGGAAT TACCTCGGAG AAGCTGTGAG CCACAATGCA 
TCGCTGGAAG TTGCAATTTT GAGAGACGAC TTCAGACAAA ACCCTTCAGA TGTAATGGTA 
GCGGTGGGGG AGCCTGCAGT AATGGAATGC CAGCCGCCCA GAGGTCATCC TGAGCCGACA 
ATATCCTGGA AAAAAGATGG GACGCCAATA GACGATGATA AGGATGAAAG AATAACTATA 
AGAGGAGGGA AACTTATGAT CACCTACACC AGGAAAAGCG ATGCTGGAAA ATATGTGTGT 
GTTGGAACCA ATATGGTGGG AGAACGGGAA AGCGAAGTAG CAGAACTTAC TGTTTTAGAA 
AGGCCATCAT TTGTGAGGAG ACCCAGTAAT ATGGCAGTTA CCGTAGATGA TAGCGTTGAA 
TTCAAATGTG AGGCCCGGGG TGATCCTGTC CCTACTGTTC GATGGAGGAA AGATGATGGG 
GATCTGCCTA AATCAAGATA TGAAATCCGT GATGACCACA CCTTGAAGAT AAGGAAGGTC 
ACAGCAGGGG ACATGGGATC CTATACTTGT GTTGCAGAGA ACATGGTCGG AAAAGCGGAA 
GCTTCGGCCG TCCTAACAGT TCAAGTTGTG TCTGAGCCTC CGAATTTTGT TGTCAAACCT 
CGTGACCAAG TTGTTGCTCT GGGACGCACA GTCACATTTC AGTGTGAAGC CACCGGAAAC 
CCCCAGCCAG CCATTTTTTG GCAAAAGGAG GGAAGCCAGA ACCTTCTCTT TTCCTACCAA 
CCTCCACAAT CGTCGAGCCG CTTCTCCGTG TCCCAGACTG GTGACCTGAC TATAACCACC 
GTTCAGAAGT CAGACGTGGG CTACTACATC TGCCAGACTC TCAACGTTGC CGGGAGCATT 
ACAACAAAAG CCTTCTTAGA AGTTACCGAT GTGGTGGCCG ACCGTCCTCC TCCAGTCATT 
CGTCAGGGAC CCGTGAATCA GACAGTAGCT GTTGATGGCA CCTTAATAAT GAACTGTATC 
GCCAGCGGCA TTCCTGTTCC CACAATCCAC TGGAGAAAGG ACGGGATCCT GATACCGACC 
CAAGACTCTC GCATTAAACA GCAGGACTCA GGAGCTCTGC AGATCCGTTA CGCCAAGCTG 
AGTGATACCG GTCGGTACAC CTGCACTGCC TCCACTACTA GCGGGGAAGC AAACTGGAGC 
GCGTACATAG AAGTCCAAGA ATTTGGAGTT CCGGTTCAAC CACCCAGAGC CACTGATCCC 
AACCTGATTC CAAGTGCTCC ATCAAAACCA GAAGTCACGG ACGTCAGCAA AAATACTGTA 
ACTTTATCCT GGCAGCCAAA CCTCAACTCA GGTGCTACTC CTACGTCGTA CCTCATAGAG 
GCCTTCAGCC ATGCGTCTGG AAGCAGCTGG CAAACAGTGG CAGAAAATGT CAAGACCGAT 
TCCTTTGCAG TTAAAGCACT GAAGCCCAAT GCAATCTACC TCTTCCTCGT GAGAGCAGTA 
AACGCATACG GATTAAGTGA TCCAAGCCAA ATATCCGATC CTGTAAAAAC ACAAGATATT 
CCTCCTACAA GTCAAGGAGT GGATCACAAA CAGGTGCAGA GAGAACTGGG CGATGTTGTG 
CTACACCTTC ATAACCCAAC AATACTTTCC TCTTCCTCCA TCGAAGTACA TTGGACTGTG 
GACCAGCAAT CACAGTACAT CCAAGGATAC AAGATCCTCT ACAGGCCAAG CGCAGAGAAT 
CGAGGCGAAT CTGCATGGTC CATATTTGAA GTCAGGACTC CGGCCAAAAA CAGCGCAGTT 
ATTCCTGAAC TGAGAAAAGG CGTTAACTAC GAGATCAAAG CCCGCCCCTT TTTTAATGAG 
TTTCAAGGAG CTGATAGTGA AGTGCGGTTC GCTAAGACAA TGGAAGAAGC ACCCAGTGCT 
CCCCCGCAGA GCGTTACTGT AACCAAGAAT GATGGGAATG GAACTGCTAT TGTTGTGGCA 
TGGCAGCCTC CCCCTGAAGA TACTCAGAAT GGGATGGTCC AGGAATACAA GGTTTGGTGC 
CTGGGGAATG AGAGTCGCTA TCACATAAAC AAGACTGTCG ATGGCTCTAC CCTTTCCGTG 
GTCATCCCGT CCTTAGTCCC TGGTATCAAA TACAGCGTGG AGGTGGCAGC CAGCACGGGC 
GCAGGATCCG GTGTGAAAAG TGAACCCAAC TACATCCAGT TAGATTCCTT TGGAAACCCG 
GTGCCTCCGG GGGACCAAGT CAGCCTGGCC CAGCAGATAT CCGATGTGGT GAAGCAGCCG 
GCATTCATAG CAGGGATTGG TGCTGCCTGC TGGATAATCC TCATGGTATT CAGTATCTGG 
TTGTACCGGC ACAGAAAGAA ACGCAATGGG CTCACCAGTT CCTATGCTGG AATACGGAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTG GCGTACCAGC GAGGAGGGGA AGCTGTAAGC 
AGCGCAGGCC GGCCTGGGCT CTTAAATATT TCTGAGCCTG CTAATCAGCC GTGGCTCGCA 
GATACCTGGC CAAACACTGG AAACAATCAC AACGAATGCC CCATCAACTG CTGCACCTCC 
GGGAACGGCA ACAGCGACAG CAACCTCGCC ACTTACAGCC GCCCAGCTGA CTGCATAGCG 
AATTACAACA ACCAGTTGGA CAGCAAGCAG ACAAACCTGA TGGTGCCCGA CTCGGCTGTT 
TATGGGGATG TAGACTTAAG CAACAAGATC AATGAGATGA AAACCTTCAA CAGCCCCAAT 
CTGAAAGATG GGAGGTTTGT CAATCCTGCC GGCCAGCCCA CCCCTTATGC CACCACTCAG 
CTCATCCAGT CCAACATAAG CAACAACATC AACACGTCTG CCGGTGATGT GGATGAAAAA 
CACTGGAAAC CCCCGTGCCA ACAGAAACCG GATGTTACCC CAATGCAGTA CAACATCATG 
GAGCAAAACA AGATCAACAA AGATTACCGA GCTTCGGACA CCATTCCTTC CACCATTCCC 
TACAACCAGT CCCATGATCA GAATGCCGGA GGGTCATACA ACAGCTCAGA CCGGGGAAGT 
AGCACATCTG GGAGCCAAGG TCATAAGAAG AGTACTCGTA CCCCGAAAGT ATCAAAGCAG 
GCTGGCATGA ACTGGGCAGA TCTTTTGCCA CCACCACCTG CTCACCCCCC ACCACATGGT 
GCCACTGAAG ACTACACTTT GTCAGCAGAT GAAAGCTATG ACAGAGAGTT ACCTTGCCCA 
ATACCACCTA CAAGAATGTA TCTTCAGCAA GACGAACTGG AAGAGGAAGA TGAAAGGGGT 
CCCACACCTC CTATTAGAGG AGCAGCTTCT TCTCCGGCTG CTGTGTCCTA CAGCCATCAG 
TCCACTGCCA CTCTTACCCC ATCACCTCAG GACGAACTCC AGCCAATGCT TCAGGACTGT 
CCCGAGGAGA TCGGTCATAC TCAGCATCAG CTAGAAAGAA GGCGTCATCA AATTAGTCCT 
CCTCCACCTC CAAGGCCCAT TTCCCCGCCT CATACATATG GTTATATTTC TGGACCAATA 
GTCTCCGATA TGGATACTGA TGTTCCTGAA GAGGAAGGGG ATGATGGGGA AATTGAAGTC 
ACCAAGATGC AAAGTCGCAG ACTTCTTTTA CGTGGCCTGG AGCAGACTCC TGCTTCCAGT 
GTTGGAGACC TAGAAAGTTC TGTGACTGGT TCCATGATCA ATGGATGGGG CTCAGCTTCT 
GAAGAAGACA ATGTTTCCAG TGGACGTTCC AGTGTCAGTT CATCGGATGG ATCATTTTTC 
ACGGATGCGG ATTTTGCACA GGCTGTCGCC GCAGCCGCAG AATATGCTGG GCTTAAAGTA 
GCAAGGCATC AAATGCAGGA AGCAGCAGGA GTGTCGCGTC TTTGCCTCCC AGGTCGAAGA 
CCTTTCCACG CGTCTCACTG TCCAAGACCA ACAAGCCCAG TGTCAACAGA CAGCAACATG 
AGCACCGCTG TCATACAGAA AAGCCGTCCT GCCAAAAAAC AAAAACACCA GCAGGGACAT 
CTTCGGAAAG ATGTCTACAC AGATGATTTG CCACCACCGC CACTGCCACC TCCTGCTATC 
AAATCTCCAA CTGGTCCATC CAAAGCACAA CTAGAAGGAC GGCCTGGCCT TTCACAGAAG 
TACCTTGTTG CAGAACAAAG ACTGGAAAGG CCAGGAGAGA GGAAAGGTGT CAGCTACAGG 
GGCAGGGAAG GTCCTGACAG CAGACAACAA CATGCAGAAG CAAGAACCAA TTCAGGGGAG 
AGAAGAGAGT CTTCGGAACA GCAACTTGAT GGAAAGATGA GAAAAGGGAA AGCTTCAAAG 
TGGGATGGGG CACCTATAGT AGCAAATCAA ATACAAGAGG ATATATTACC CTATTGCAAA 
CCAACATTCC CCACTTCAAA CAACCCTCGT GATCCCAGTT CCTCCAGCTC AATGTCCTCA 
AGAGGATCTG GTGGACGACG GAGGGGAGAA CAAACAACAG GTCGCAGAAA TGCAGCAGAG 
ATGCAGCTCT TAGGAAATTA TGAACGAGGG GTTGATGAAG ATGAAATAGA GGAAACAGAA 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956263.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811717.1 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X8, mRNA.

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CloneID	OXa30334
Clone ID Related Accession (Same CDS sequence)	XM_012956263.2
Accession Version	XM_012956263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCAG AGTTGGCTCA CTTTTTCCTG CTCTGTGGAA TCATGTGCTT GACTTCAGGG 
TCACGTCTTC GGCAAGAAGA TTTCCCACCG CGAATTGTTG AGCATCCCTC AGACCTAATT 
GTCTCCAAAG GTGAACCAGC AACGCTGAAC TGCAAGGCCG AAGGGAGGCC GACTCCAACC 
ATTGAGTGGT ATAAAGGAGG AGACAAAGTA GAGACAGATA AAGACGATCC TCGCTCTCAC 
CGAATGTTGT TGCCCAGTGG ATCACTATTT TTTTTACGTA TCGTGCATGG ACGCAAAATC 
AGGCCGGATG AAGGGGTCTA TGTTTGTGTT GCAAGGAATT ACCTCGGAGA AGCTGTGAGC 
CACAATGCAT CGCTGGAAGT TGCAATTTTG AGAGACGACT TCAGACAAAA CCCTTCAGAT 
GTAATGGTAG CGGTGGGGGA GCCTGCAGTA ATGGAATGCC AGCCGCCCAG AGGTCATCCT 
GAGCCGACAA TATCCTGGAA AAAAGATGGG ACGCCAATAG ACGATGATAA GGATGAAAGA 
ATAACTATAA GAGGAGGGAA ACTTATGATC ACCTACACCA GGAAAAGCGA TGCTGGAAAA 
TATGTGTGTG TTGGAACCAA TATGGTGGGA GAACGGGAAA GCGAAGTAGC AGAACTTACT 
GTTTTAGAAA GGCCATCATT TGTGAGGAGA CCCAGTAATA TGGCAGTTAC CGTAGATGAT 
AGCGTTGAAT TCAAATGTGA GGCCCGGGGT GATCCTGTCC CTACTGTTCG ATGGAGGAAA 
GATGATGGGG ATCTGCCTAA ATCAAGATAT GAAATCCGTG ATGACCACAC CTTGAAGATA 
AGGAAGGTCA CAGCAGGGGA CATGGGATCC TATACTTGTG TTGCAGAGAA CATGGTCGGA 
AAAGCGGAAG CTTCGGCCGT CCTAACAGTT CAAGTTGTGT CTGAGCCTCC GAATTTTGTT 
GTCAAACCTC GTGACCAAGT TGTTGCTCTG GGACGCACAG TCACATTTCA GTGTGAAGCC 
ACCGGAAACC CCCAGCCAGC CATTTTTTGG CAAAAGGAGG GAAGCCAGAA CCTTCTCTTT 
TCCTACCAAC CTCCACAATC GTCGAGCCGC TTCTCCGTGT CCCAGACTGG TGACCTGACT 
ATAACCACCG TTCAGAAGTC AGACGTGGGC TACTACATCT GCCAGACTCT CAACGTTGCC 
GGGAGCATTA CAACAAAAGC CTTCTTAGAA GTTACCGATG TGGTGGCCGA CCGTCCTCCT 
CCAGTCATTC GTCAGGGACC CGTGAATCAG ACAGTAGCTG TTGATGGCAC CTTAATAATG 
AACTGTATCG CCAGCGGCAT TCCTGTTCCC ACAATCCACT GGAGAAAGGA CGGGATCCTG 
ATACCGACCC AAGACTCTCG CATTAAACAG CAGGACTCAG GAGCTCTGCA GATCCGTTAC 
GCCAAGCTGA GTGATACCGG TCGGTACACC TGCACTGCCT CCACTACTAG CGGGGAAGCA 
AACTGGAGCG CGTACATAGA AGTCCAAGAA TTTGGAGTTC CGGTTCAACC ACCCAGAGCC 
ACTGATCCCA ACCTGATTCC AAGTGCTCCA TCAAAACCAG AAGTCACGGA CGTCAGCAAA 
AATACTGTAA CTTTATCCTG GCAGCCAAAC CTCAACTCAG GTGCTACTCC TACGTCGTAC 
CTCATAGAGG CCTTCAGCCA TGCGTCTGGA AGCAGCTGGC AAACAGTGGC AGAAAATGTC 
AAGACCGATT CCTTTGCAGT TAAAGCACTG AAGCCCAATG CAATCTACCT CTTCCTCGTG 
AGAGCAGTAA ACGCATACGG ATTAAGTGAT CCAAGCCAAA TATCCGATCC TGTAAAAACA 
CAAGATATTC CTCCTACAAG TCAAGGAGTG GATCACAAAC AGGTGCAGAG AGAACTGGGC 
GATGTTGTGC TACACCTTCA TAACCCAACA ATACTTTCCT CTTCCTCCAT CGAAGTACAT 
TGGACTGTGG ACCAGCAATC ACAGTACATC CAAGGATACA AGATCCTCTA CAGGCCAAGC 
GCAGAGAATC GAGGCGAATC TGCATGGTCC ATATTTGAAG TCAGGACTCC GGCCAAAAAC 
AGCGCAGTTA TTCCTGAACT GAGAAAAGGC GTTAACTACG AGATCAAAGC CCGCCCCTTT 
TTTAATGAGT TTCAAGGAGC TGATAGTGAA GTGCGGTTCG CTAAGACAAT GGAAGAAGCA 
CCCAGTGCTC CCCCGCAGAG CGTTACTGTA ACCAAGAATG ATGGGAATGG AACTGCTATT 
GTTGTGGCAT GGCAGCCTCC CCCTGAAGAT ACTCAGAATG GGATGGTCCA GGAATACAAG 
GTTTGGTGCC TGGGGAATGA GAGTCGCTAT CACATAAACA AGACTGTCGA TGGCTCTACC 
CTTTCCGTGG TCATCCCGTC CTTAGTCCCT GGTATCAAAT ACAGCGTGGA GGTGGCAGCC 
AGCACGGGCG CAGGATCCGG TGTGAAAAGT GAACCCAACT ACATCCAGTT AGATTCCTTT 
GGAAACCCGG TGCCTCCGGG GGACCAAGTC AGCCTGGCCC AGCAGATATC CGATGTGGTG 
AAGCAGCCGG CATTCATAGC AGGGATTGGT GCTGCCTGCT GGATAATCCT CATGGTATTC 
AGTATCTGGT TGTACCGGCA CAGAAAGAAA CGCAATGGGC TCACCAGTTC CTATGCTGGA 
ATACGGAAAG TCCCGTCTTT TACCTTCACA CCAACAGTGG CGTACCAGCG AGGAGGGGAA 
GCTGTAAGCA GCGCAGGCCG GCCTGGGCTC TTAAATATTT CTGAGCCTGC TAATCAGCCG 
TGGCTCGCAG ATACCTGGCC AAACACTGGA AACAATCACA ACGAATGCCC CATCAACTGC 
TGCACCTCCG GGAACGGCAA CAGCGACAGC AACCTCGCCA CTTACAGCCG CCCAGCTGAC 
TGCATAGCGA ATTACAACAA CCAGTTGGAC AGCAAGCAGA CAAACCTGAT GGTGCCCGAC 
TCGGCTGTTT ATGGGGATGT AGACTTAAGC AACAAGATCA ATGAGATGAA AACCTTCAAC 
AGCCCCAATC TGAAAGATGG GAGGTTTGTC AATCCTGCCG GCCAGCCCAC CCCTTATGCC 
ACCACTCAGC TCATCCAGTC CAACATAAGC AACAACATCA ACACGTCTGC CGGTGATGTG 
GATGAAAAAC ACTGGAAACC CCCGTGCCAA CAGAAACCGG ATGTTACCCC AATGCAGTAC 
AACATCATGG AGCAAAACAA GATCAACAAA GATTACCGAG CTTCGGACAC CATTCCTTCC 
ACCATTCCCT ACAACCAGTC CCATGATCAG AATGCCGGAG GGTCATACAA CAGCTCAGAC 
CGGGGAAGTA GCACATCTGG GAGCCAAGGT CATAAGAAGA GTACTCGTAC CCCGAAAGTA 
TCAAAGCAGG CTGGCATGAA CTGGGCAGAT CTTTTGCCAC CACCACCTGC TCACCCCCCA 
CCACATGGTG CCACTGAAGA CTACACTTTG TCAGCAGATG AAAGCTATGA CAGAGAGTTA 
CCTTGCCCAA TACCACCTAC AAGAATGTAT CTTCAGCAAG ACGAACTGGA AGAGGAAGAT 
GAAAGGGGTC CCACACCTCC TATTAGAGGA GCAGCTTCTT CTCCGGCTGC TGTGTCCTAC 
AGCCATCAGT CCACTGCCAC TCTTACCCCA TCACCTCAGG ACGAACTCCA GCCAATGCTT 
CAGGACTGTC CCGAGGAGAT CGGTCATACT CAGCATCAGC TAGAAAGAAG GCGTCATCAA 
ATTAGTCCTC CTCCACCTCC AAGGCCCATT TCCCCGCCTC ATACATATGG TTATATTTCT 
GGACCAATAG TCTCCGATAT GGATACTGAT GTTCCTGAAG AGGAAGGGGA TGATGGGGAA 
ATTGAAGTCA CCAAGATGCA AAGTCGCAGA CTTCTTTTAC GTGGCCTGGA GCAGACTCCT 
GCTTCCAGTG TTGGAGACCT AGAAAGTTCT GTGACTGGTT CCATGATCAA TGGATGGGGC 
TCAGCTTCTG AAGAAGACAA TGTTTCCAGT GGACGTTCCA GTGTCAGTTC ATCGGATGGA 
TCATTTTTCA CGGATGCGGA TTTTGCACAG GCTGTCGCCG CAGCCGCAGA ATATGCTGGG 
CTTAAAGTAG CAAGGCATCA AATGCAGGAA GCAGCAGGAG TGTCGCGTCT TTGCCTCCCA 
GGTCGAAGAC CTTTCCACGC GTCTCACTGT CCAAGACCAA CAAGCCCAGT GTCAACAGAC 
AGCAACATGA GCACCGCTGT CATACAGAAA AGCCGTCCTG CCAAAAAACA AAAACACCAG 
CAGGGACATC TTCGGAAAGA TGTCTACACA GATGATTTGC CACCACCGCC ACTGCCACCT 
CCTGCTATCA AATCTCCAAC TGGTCCATCC AAAGCACAAC TAGAAGGACG GCCTGGCCTT 
TCACAGAAGT ACCTTGTTGC AGAACAAAGA CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC 
AGCTACAGGG GCAGGGAAGG TCCTGACAGC AGACAACAAC ATGCAGAAGC AAGAACCAAT 
TCAGGGGAGA GAAGAGAGTC TTCGGAACAG CAACTTGATG GAAAGATGAG AAAAGGGAAA 
GCTTCAAAGT GGGATGGGGC ACCTATAGTA GCAAATCAAA TACAAGAGGA TATATTACCC 
TATTGCAAAC CAACATTCCC CACTTCAAAC AACCCTCGTG ATCCCAGTTC CTCCAGCTCA 
ATGTCCTCAA GAGGATCTGG TGGACGACGG AGGGGAGAAC AAACAACAGG TCGCAGAAAT 
GCAGCAGAGA TGCAGCTCTT AGGAAATTAT GAACGAGGGG TTGATGAAGA TGAAATAGAG 
GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811717.1
CDS	1472..6346
Translation	MLSELAHFFLLCGIMCLTSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQVVSEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISEPANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGVSRLCLPGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956263.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956263.2

ORF Insert Sequence:

ATGCTCTCAG AGTTGGCTCA CTTTTTCCTG CTCTGTGGAA TCATGTGCTT GACTTCAGGG 
TCACGTCTTC GGCAAGAAGA TTTCCCACCG CGAATTGTTG AGCATCCCTC AGACCTAATT 
GTCTCCAAAG GTGAACCAGC AACGCTGAAC TGCAAGGCCG AAGGGAGGCC GACTCCAACC 
ATTGAGTGGT ATAAAGGAGG AGACAAAGTA GAGACAGATA AAGACGATCC TCGCTCTCAC 
CGAATGTTGT TGCCCAGTGG ATCACTATTT TTTTTACGTA TCGTGCATGG ACGCAAAATC 
AGGCCGGATG AAGGGGTCTA TGTTTGTGTT GCAAGGAATT ACCTCGGAGA AGCTGTGAGC 
CACAATGCAT CGCTGGAAGT TGCAATTTTG AGAGACGACT TCAGACAAAA CCCTTCAGAT 
GTAATGGTAG CGGTGGGGGA GCCTGCAGTA ATGGAATGCC AGCCGCCCAG AGGTCATCCT 
GAGCCGACAA TATCCTGGAA AAAAGATGGG ACGCCAATAG ACGATGATAA GGATGAAAGA 
ATAACTATAA GAGGAGGGAA ACTTATGATC ACCTACACCA GGAAAAGCGA TGCTGGAAAA 
TATGTGTGTG TTGGAACCAA TATGGTGGGA GAACGGGAAA GCGAAGTAGC AGAACTTACT 
GTTTTAGAAA GGCCATCATT TGTGAGGAGA CCCAGTAATA TGGCAGTTAC CGTAGATGAT 
AGCGTTGAAT TCAAATGTGA GGCCCGGGGT GATCCTGTCC CTACTGTTCG ATGGAGGAAA 
GATGATGGGG ATCTGCCTAA ATCAAGATAT GAAATCCGTG ATGACCACAC CTTGAAGATA 
AGGAAGGTCA CAGCAGGGGA CATGGGATCC TATACTTGTG TTGCAGAGAA CATGGTCGGA 
AAAGCGGAAG CTTCGGCCGT CCTAACAGTT CAAGTTGTGT CTGAGCCTCC GAATTTTGTT 
GTCAAACCTC GTGACCAAGT TGTTGCTCTG GGACGCACAG TCACATTTCA GTGTGAAGCC 
ACCGGAAACC CCCAGCCAGC CATTTTTTGG CAAAAGGAGG GAAGCCAGAA CCTTCTCTTT 
TCCTACCAAC CTCCACAATC GTCGAGCCGC TTCTCCGTGT CCCAGACTGG TGACCTGACT 
ATAACCACCG TTCAGAAGTC AGACGTGGGC TACTACATCT GCCAGACTCT CAACGTTGCC 
GGGAGCATTA CAACAAAAGC CTTCTTAGAA GTTACCGATG TGGTGGCCGA CCGTCCTCCT 
CCAGTCATTC GTCAGGGACC CGTGAATCAG ACAGTAGCTG TTGATGGCAC CTTAATAATG 
AACTGTATCG CCAGCGGCAT TCCTGTTCCC ACAATCCACT GGAGAAAGGA CGGGATCCTG 
ATACCGACCC AAGACTCTCG CATTAAACAG CAGGACTCAG GAGCTCTGCA GATCCGTTAC 
GCCAAGCTGA GTGATACCGG TCGGTACACC TGCACTGCCT CCACTACTAG CGGGGAAGCA 
AACTGGAGCG CGTACATAGA AGTCCAAGAA TTTGGAGTTC CGGTTCAACC ACCCAGAGCC 
ACTGATCCCA ACCTGATTCC AAGTGCTCCA TCAAAACCAG AAGTCACGGA CGTCAGCAAA 
AATACTGTAA CTTTATCCTG GCAGCCAAAC CTCAACTCAG GTGCTACTCC TACGTCGTAC 
CTCATAGAGG CCTTCAGCCA TGCGTCTGGA AGCAGCTGGC AAACAGTGGC AGAAAATGTC 
AAGACCGATT CCTTTGCAGT TAAAGCACTG AAGCCCAATG CAATCTACCT CTTCCTCGTG 
AGAGCAGTAA ACGCATACGG ATTAAGTGAT CCAAGCCAAA TATCCGATCC TGTAAAAACA 
CAAGATATTC CTCCTACAAG TCAAGGAGTG GATCACAAAC AGGTGCAGAG AGAACTGGGC 
GATGTTGTGC TACACCTTCA TAACCCAACA ATACTTTCCT CTTCCTCCAT CGAAGTACAT 
TGGACTGTGG ACCAGCAATC ACAGTACATC CAAGGATACA AGATCCTCTA CAGGCCAAGC 
GCAGAGAATC GAGGCGAATC TGCATGGTCC ATATTTGAAG TCAGGACTCC GGCCAAAAAC 
AGCGCAGTTA TTCCTGAACT GAGAAAAGGC GTTAACTACG AGATCAAAGC CCGCCCCTTT 
TTTAATGAGT TTCAAGGAGC TGATAGTGAA GTGCGGTTCG CTAAGACAAT GGAAGAAGCA 
CCCAGTGCTC CCCCGCAGAG CGTTACTGTA ACCAAGAATG ATGGGAATGG AACTGCTATT 
GTTGTGGCAT GGCAGCCTCC CCCTGAAGAT ACTCAGAATG GGATGGTCCA GGAATACAAG 
GTTTGGTGCC TGGGGAATGA GAGTCGCTAT CACATAAACA AGACTGTCGA TGGCTCTACC 
CTTTCCGTGG TCATCCCGTC CTTAGTCCCT GGTATCAAAT ACAGCGTGGA GGTGGCAGCC 
AGCACGGGCG CAGGATCCGG TGTGAAAAGT GAACCCAACT ACATCCAGTT AGATTCCTTT 
GGAAACCCGG TGCCTCCGGG GGACCAAGTC AGCCTGGCCC AGCAGATATC CGATGTGGTG 
AAGCAGCCGG CATTCATAGC AGGGATTGGT GCTGCCTGCT GGATAATCCT CATGGTATTC 
AGTATCTGGT TGTACCGGCA CAGAAAGAAA CGCAATGGGC TCACCAGTTC CTATGCTGGA 
ATACGGAAAG TCCCGTCTTT TACCTTCACA CCAACAGTGG CGTACCAGCG AGGAGGGGAA 
GCTGTAAGCA GCGCAGGCCG GCCTGGGCTC TTAAATATTT CTGAGCCTGC TAATCAGCCG 
TGGCTCGCAG ATACCTGGCC AAACACTGGA AACAATCACA ACGAATGCCC CATCAACTGC 
TGCACCTCCG GGAACGGCAA CAGCGACAGC AACCTCGCCA CTTACAGCCG CCCAGCTGAC 
TGCATAGCGA ATTACAACAA CCAGTTGGAC AGCAAGCAGA CAAACCTGAT GGTGCCCGAC 
TCGGCTGTTT ATGGGGATGT AGACTTAAGC AACAAGATCA ATGAGATGAA AACCTTCAAC 
AGCCCCAATC TGAAAGATGG GAGGTTTGTC AATCCTGCCG GCCAGCCCAC CCCTTATGCC 
ACCACTCAGC TCATCCAGTC CAACATAAGC AACAACATCA ACACGTCTGC CGGTGATGTG 
GATGAAAAAC ACTGGAAACC CCCGTGCCAA CAGAAACCGG ATGTTACCCC AATGCAGTAC 
AACATCATGG AGCAAAACAA GATCAACAAA GATTACCGAG CTTCGGACAC CATTCCTTCC 
ACCATTCCCT ACAACCAGTC CCATGATCAG AATGCCGGAG GGTCATACAA CAGCTCAGAC 
CGGGGAAGTA GCACATCTGG GAGCCAAGGT CATAAGAAGA GTACTCGTAC CCCGAAAGTA 
TCAAAGCAGG CTGGCATGAA CTGGGCAGAT CTTTTGCCAC CACCACCTGC TCACCCCCCA 
CCACATGGTG CCACTGAAGA CTACACTTTG TCAGCAGATG AAAGCTATGA CAGAGAGTTA 
CCTTGCCCAA TACCACCTAC AAGAATGTAT CTTCAGCAAG ACGAACTGGA AGAGGAAGAT 
GAAAGGGGTC CCACACCTCC TATTAGAGGA GCAGCTTCTT CTCCGGCTGC TGTGTCCTAC 
AGCCATCAGT CCACTGCCAC TCTTACCCCA TCACCTCAGG ACGAACTCCA GCCAATGCTT 
CAGGACTGTC CCGAGGAGAT CGGTCATACT CAGCATCAGC TAGAAAGAAG GCGTCATCAA 
ATTAGTCCTC CTCCACCTCC AAGGCCCATT TCCCCGCCTC ATACATATGG TTATATTTCT 
GGACCAATAG TCTCCGATAT GGATACTGAT GTTCCTGAAG AGGAAGGGGA TGATGGGGAA 
ATTGAAGTCA CCAAGATGCA AAGTCGCAGA CTTCTTTTAC GTGGCCTGGA GCAGACTCCT 
GCTTCCAGTG TTGGAGACCT AGAAAGTTCT GTGACTGGTT CCATGATCAA TGGATGGGGC 
TCAGCTTCTG AAGAAGACAA TGTTTCCAGT GGACGTTCCA GTGTCAGTTC ATCGGATGGA 
TCATTTTTCA CGGATGCGGA TTTTGCACAG GCTGTCGCCG CAGCCGCAGA ATATGCTGGG 
CTTAAAGTAG CAAGGCATCA AATGCAGGAA GCAGCAGGAG TGTCGCGTCT TTGCCTCCCA 
GGTCGAAGAC CTTTCCACGC GTCTCACTGT CCAAGACCAA CAAGCCCAGT GTCAACAGAC 
AGCAACATGA GCACCGCTGT CATACAGAAA AGCCGTCCTG CCAAAAAACA AAAACACCAG 
CAGGGACATC TTCGGAAAGA TGTCTACACA GATGATTTGC CACCACCGCC ACTGCCACCT 
CCTGCTATCA AATCTCCAAC TGGTCCATCC AAAGCACAAC TAGAAGGACG GCCTGGCCTT 
TCACAGAAGT ACCTTGTTGC AGAACAAAGA CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC 
AGCTACAGGG GCAGGGAAGG TCCTGACAGC AGACAACAAC ATGCAGAAGC AAGAACCAAT 
TCAGGGGAGA GAAGAGAGTC TTCGGAACAG CAACTTGATG GAAAGATGAG AAAAGGGAAA 
GCTTCAAAGT GGGATGGGGC ACCTATAGTA GCAAATCAAA TACAAGAGGA TATATTACCC 
TATTGCAAAC CAACATTCCC CACTTCAAAC AACCCTCGTG ATCCCAGTTC CTCCAGCTCA 
ATGTCCTCAA GAGGATCTGG TGGACGACGG AGGGGAGAAC AAACAACAGG TCGCAGAAAT 
GCAGCAGAGA TGCAGCTCTT AGGAAATTAT GAACGAGGGG TTGATGAAGA TGAAATAGAG 
GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo1 ( XM_012956264.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811718.1 Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X9, mRNA.

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CloneID	OXa30335
Clone ID Related Accession (Same CDS sequence)	XM_012956264.2
Accession Version	XM_012956264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956264.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCAG AGTTGGCTCA CTTTTTCCTG CTCTGTGGAA TCATGTGCTT GACTTCAGGG 
TCACGTCTTC GGCAAGAAGA TTTCCCACCG CGAATTGTTG AGCATCCCTC AGACCTAATT 
GTCTCCAAAG GTGAACCAGC AACGCTGAAC TGCAAGGCCG AAGGGAGGCC GACTCCAACC 
ATTGAGTGGT ATAAAGGAGG AGACAAAGTA GAGACAGATA AAGACGATCC TCGCTCTCAC 
CGAATGTTGT TGCCCAGTGG ATCACTATTT TTTTTACGTA TCGTGCATGG ACGCAAAATC 
AGGCCGGATG AAGGGGTCTA TGTTTGTGTT GCAAGGAATT ACCTCGGAGA AGCTGTGAGC 
CACAATGCAT CGCTGGAAGT TGCAATTTTG AGAGACGACT TCAGACAAAA CCCTTCAGAT 
GTAATGGTAG CGGTGGGGGA GCCTGCAGTA ATGGAATGCC AGCCGCCCAG AGGTCATCCT 
GAGCCGACAA TATCCTGGAA AAAAGATGGG ACGCCAATAG ACGATGATAA GGATGAAAGA 
ATAACTATAA GAGGAGGGAA ACTTATGATC ACCTACACCA GGAAAAGCGA TGCTGGAAAA 
TATGTGTGTG TTGGAACCAA TATGGTGGGA GAACGGGAAA GCGAAGTAGC AGAACTTACT 
GTTTTAGAAA GGCCATCATT TGTGAGGAGA CCCAGTAATA TGGCAGTTAC CGTAGATGAT 
AGCGTTGAAT TCAAATGTGA GGCCCGGGGT GATCCTGTCC CTACTGTTCG ATGGAGGAAA 
GATGATGGGG ATCTGCCTAA ATCAAGATAT GAAATCCGTG ATGACCACAC CTTGAAGATA 
AGGAAGGTCA CAGCAGGGGA CATGGGATCC TATACTTGTG TTGCAGAGAA CATGGTCGGA 
AAAGCGGAAG CTTCGGCCGT CCTAACAGTT CAAGAGCCTC CGAATTTTGT TGTCAAACCT 
CGTGACCAAG TTGTTGCTCT GGGACGCACA GTCACATTTC AGTGTGAAGC CACCGGAAAC 
CCCCAGCCAG CCATTTTTTG GCAAAAGGAG GGAAGCCAGA ACCTTCTCTT TTCCTACCAA 
CCTCCACAAT CGTCGAGCCG CTTCTCCGTG TCCCAGACTG GTGACCTGAC TATAACCACC 
GTTCAGAAGT CAGACGTGGG CTACTACATC TGCCAGACTC TCAACGTTGC CGGGAGCATT 
ACAACAAAAG CCTTCTTAGA AGTTACCGAT GTGGTGGCCG ACCGTCCTCC TCCAGTCATT 
CGTCAGGGAC CCGTGAATCA GACAGTAGCT GTTGATGGCA CCTTAATAAT GAACTGTATC 
GCCAGCGGCA TTCCTGTTCC CACAATCCAC TGGAGAAAGG ACGGGATCCT GATACCGACC 
CAAGACTCTC GCATTAAACA GCAGGACTCA GGAGCTCTGC AGATCCGTTA CGCCAAGCTG 
AGTGATACCG GTCGGTACAC CTGCACTGCC TCCACTACTA GCGGGGAAGC AAACTGGAGC 
GCGTACATAG AAGTCCAAGA ATTTGGAGTT CCGGTTCAAC CACCCAGAGC CACTGATCCC 
AACCTGATTC CAAGTGCTCC ATCAAAACCA GAAGTCACGG ACGTCAGCAA AAATACTGTA 
ACTTTATCCT GGCAGCCAAA CCTCAACTCA GGTGCTACTC CTACGTCGTA CCTCATAGAG 
GCCTTCAGCC ATGCGTCTGG AAGCAGCTGG CAAACAGTGG CAGAAAATGT CAAGACCGAT 
TCCTTTGCAG TTAAAGCACT GAAGCCCAAT GCAATCTACC TCTTCCTCGT GAGAGCAGTA 
AACGCATACG GATTAAGTGA TCCAAGCCAA ATATCCGATC CTGTAAAAAC ACAAGATATT 
CCTCCTACAA GTCAAGGAGT GGATCACAAA CAGGTGCAGA GAGAACTGGG CGATGTTGTG 
CTACACCTTC ATAACCCAAC AATACTTTCC TCTTCCTCCA TCGAAGTACA TTGGACTGTG 
GACCAGCAAT CACAGTACAT CCAAGGATAC AAGATCCTCT ACAGGCCAAG CGCAGAGAAT 
CGAGGCGAAT CTGCATGGTC CATATTTGAA GTCAGGACTC CGGCCAAAAA CAGCGCAGTT 
ATTCCTGAAC TGAGAAAAGG CGTTAACTAC GAGATCAAAG CCCGCCCCTT TTTTAATGAG 
TTTCAAGGAG CTGATAGTGA AGTGCGGTTC GCTAAGACAA TGGAAGAAGC ACCCAGTGCT 
CCCCCGCAGA GCGTTACTGT AACCAAGAAT GATGGGAATG GAACTGCTAT TGTTGTGGCA 
TGGCAGCCTC CCCCTGAAGA TACTCAGAAT GGGATGGTCC AGGAATACAA GGTTTGGTGC 
CTGGGGAATG AGAGTCGCTA TCACATAAAC AAGACTGTCG ATGGCTCTAC CCTTTCCGTG 
GTCATCCCGT CCTTAGTCCC TGGTATCAAA TACAGCGTGG AGGTGGCAGC CAGCACGGGC 
GCAGGATCCG GTGTGAAAAG TGAACCCAAC TACATCCAGT TAGATTCCTT TGGAAACCCG 
GTGCCTCCGG GGGACCAAGT CAGCCTGGCC CAGCAGATAT CCGATGTGGT GAAGCAGCCG 
GCATTCATAG CAGGGATTGG TGCTGCCTGC TGGATAATCC TCATGGTATT CAGTATCTGG 
TTGTACCGGC ACAGAAAGAA ACGCAATGGG CTCACCAGTT CCTATGCTGG AATACGGAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTG GCGTACCAGC GAGGAGGGGA AGCTGTAAGC 
AGCGCAGGCC GGCCTGGGCT CTTAAATATT TCTGAGCCTG CTAATCAGCC GTGGCTCGCA 
GATACCTGGC CAAACACTGG AAACAATCAC AACGAATGCC CCATCAACTG CTGCACCTCC 
GGGAACGGCA ACAGCGACAG CAACCTCGCC ACTTACAGCC GCCCAGCTGA CTGCATAGCG 
AATTACAACA ACCAGTTGGA CAGCAAGCAG ACAAACCTGA TGGTGCCCGA CTCGGCTGTT 
TATGGGGATG TAGACTTAAG CAACAAGATC AATGAGATGA AAACCTTCAA CAGCCCCAAT 
CTGAAAGATG GGAGGTTTGT CAATCCTGCC GGCCAGCCCA CCCCTTATGC CACCACTCAG 
CTCATCCAGT CCAACATAAG CAACAACATC AACACGTCTG CCGGTGATGT GGATGAAAAA 
CACTGGAAAC CCCCGTGCCA ACAGAAACCG GATGTTACCC CAATGCAGTA CAACATCATG 
GAGCAAAACA AGATCAACAA AGATTACCGA GCTTCGGACA CCATTCCTTC CACCATTCCC 
TACAACCAGT CCCATGATCA GAATGCCGGA GGGTCATACA ACAGCTCAGA CCGGGGAAGT 
AGCACATCTG GGAGCCAAGG TCATAAGAAG AGTACTCGTA CCCCGAAAGT ATCAAAGCAG 
GCTGGCATGA ACTGGGCAGA TCTTTTGCCA CCACCACCTG CTCACCCCCC ACCACATGGT 
GCCACTGAAG ACTACACTTT GTCAGCAGAT GAAAGCTATG ACAGAGAGTT ACCTTGCCCA 
ATACCACCTA CAAGAATGTA TCTTCAGCAA GACGAACTGG AAGAGGAAGA TGAAAGGGGT 
CCCACACCTC CTATTAGAGG AGCAGCTTCT TCTCCGGCTG CTGTGTCCTA CAGCCATCAG 
TCCACTGCCA CTCTTACCCC ATCACCTCAG GACGAACTCC AGCCAATGCT TCAGGACTGT 
CCCGAGGAGA TCGGTCATAC TCAGCATCAG CTAGAAAGAA GGCGTCATCA AATTAGTCCT 
CCTCCACCTC CAAGGCCCAT TTCCCCGCCT CATACATATG GTTATATTTC TGGACCAATA 
GTCTCCGATA TGGATACTGA TGTTCCTGAA GAGGAAGGGG ATGATGGGGA AATTGAAGTC 
ACCAAGATGC AAAGTCGCAG ACTTCTTTTA CGTGGCCTGG AGCAGACTCC TGCTTCCAGT 
GTTGGAGACC TAGAAAGTTC TGTGACTGGT TCCATGATCA ATGGATGGGG CTCAGCTTCT 
GAAGAAGACA ATGTTTCCAG TGGACGTTCC AGTGTCAGTT CATCGGATGG ATCATTTTTC 
ACGGATGCGG ATTTTGCACA GGCTGTCGCC GCAGCCGCAG AATATGCTGG GCTTAAAGTA 
GCAAGGCATC AAATGCAGGA AGCAGCAGGA GGTCGAAGAC CTTTCCACGC GTCTCACTGT 
CCAAGACCAA CAAGCCCAGT GTCAACAGAC AGCAACATGA GCACCGCTGT CATACAGAAA 
AGCCGTCCTG CCAAAAAACA AAAACACCAG CAGGGACATC TTCGGAAAGA TGTCTACACA 
GATGATTTGC CACCACCGCC ACTGCCACCT CCTGCTATCA AATCTCCAAC TGGTCCATCC 
AAAGCACAAC TAGAAGGACG GCCTGGCCTT TCACAGAAGT ACCTTGTTGC AGAACAAAGA 
CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC AGCTACAGGG GCAGGGAAGG TCCTGACAGC 
AGACAACAAC ATGCAGAAGC AAGAACCAAT TCAGGGGAGA GAAGAGAGTC TTCGGAACAG 
CAACTTGATG GAAAGATGAG AAAAGGGAAA GCTTCAAAGT GGGATGGGGC ACCTATAGTA 
GCAAATCAAA TACAAGAGGA TATATTACCC TATTGCAAAC CAACATTCCC CACTTCAAAC 
AACCCTCGTG ATCCCAGTTC CTCCAGCTCA ATGTCCTCAA GAGGATCTGG TGGACGACGG 
AGGGGAGAAC AAACAACAGG TCGCAGAAAT GCAGCAGAGA TGCAGCTCTT AGGAAATTAT 
GAACGAGGGG TTGATGAAGA TGAAATAGAG GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811718.1
CDS	1471..6315
Translation	MLSELAHFFLLCGIMCLTSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIASGIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQISDPVKTQDIPPTSQGVDHKQVQRELGDVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSAENRGESAWSIFEVRTPAKNSAVIPELRKGVNYEIKARPFFNEFQGADSEVRFAKTMEEAPSAPPQSVTVTKNDGNGTAIVVAWQPPPEDTQNGMVQEYKVWCLGNESRYHINKTVDGSTLSVVIPSLVPGIKYSVEVAASTGAGSGVKSEPNYIQLDSFGNPVPPGDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSSYAGIRKVPSFTFTPTVAYQRGGEAVSSAGRPGLLNISEPANQPWLADTWPNTGNNHNECPINCCTSGNGNSDSNLATYSRPADCIANYNNQLDSKQTNLMVPDSAVYGDVDLSNKINEMKTFNSPNLKDGRFVNPAGQPTPYATTQLIQSNISNNINTSAGDVDEKHWKPPCQQKPDVTPMQYNIMEQNKINKDYRASDTIPSTIPYNQSHDQNAGGSYNSSDRGSSTSGSQGHKKSTRTPKVSKQAGMNWADLLPPPPAHPPPHGATEDYTLSADESYDRELPCPIPPTRMYLQQDELEEEDERGPTPPIRGAASSPAAVSYSHQSTATLTPSPQDELQPMLQDCPEEIGHTQHQLERRRHQISPPPPPRPISPPHTYGYISGPIVSDMDTDVPEEEGDDGEIEVTKMQSRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNVSSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARHQMQEAAGGRRPFHASHCPRPTSPVSTDSNMSTAVIQKSRPAKKQKHQQGHLRKDVYTDDLPPPPLPPPAIKSPTGPSKAQLEGRPGLSQKYLVAEQRLERPGERKGVSYRGREGPDSRQQHAEARTNSGERRESSEQQLDGKMRKGKASKWDGAPIVANQIQEDILPYCKPTFPTSNNPRDPSSSSSMSSRGSGGRRRGEQTTGRRNAAEMQLLGNYERGVDEDEIEETES

Target ORF information:

RefSeq Version	XM_012956264.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 1 (robo1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956264.2

ORF Insert Sequence:

ATGCTCTCAG AGTTGGCTCA CTTTTTCCTG CTCTGTGGAA TCATGTGCTT GACTTCAGGG 
TCACGTCTTC GGCAAGAAGA TTTCCCACCG CGAATTGTTG AGCATCCCTC AGACCTAATT 
GTCTCCAAAG GTGAACCAGC AACGCTGAAC TGCAAGGCCG AAGGGAGGCC GACTCCAACC 
ATTGAGTGGT ATAAAGGAGG AGACAAAGTA GAGACAGATA AAGACGATCC TCGCTCTCAC 
CGAATGTTGT TGCCCAGTGG ATCACTATTT TTTTTACGTA TCGTGCATGG ACGCAAAATC 
AGGCCGGATG AAGGGGTCTA TGTTTGTGTT GCAAGGAATT ACCTCGGAGA AGCTGTGAGC 
CACAATGCAT CGCTGGAAGT TGCAATTTTG AGAGACGACT TCAGACAAAA CCCTTCAGAT 
GTAATGGTAG CGGTGGGGGA GCCTGCAGTA ATGGAATGCC AGCCGCCCAG AGGTCATCCT 
GAGCCGACAA TATCCTGGAA AAAAGATGGG ACGCCAATAG ACGATGATAA GGATGAAAGA 
ATAACTATAA GAGGAGGGAA ACTTATGATC ACCTACACCA GGAAAAGCGA TGCTGGAAAA 
TATGTGTGTG TTGGAACCAA TATGGTGGGA GAACGGGAAA GCGAAGTAGC AGAACTTACT 
GTTTTAGAAA GGCCATCATT TGTGAGGAGA CCCAGTAATA TGGCAGTTAC CGTAGATGAT 
AGCGTTGAAT TCAAATGTGA GGCCCGGGGT GATCCTGTCC CTACTGTTCG ATGGAGGAAA 
GATGATGGGG ATCTGCCTAA ATCAAGATAT GAAATCCGTG ATGACCACAC CTTGAAGATA 
AGGAAGGTCA CAGCAGGGGA CATGGGATCC TATACTTGTG TTGCAGAGAA CATGGTCGGA 
AAAGCGGAAG CTTCGGCCGT CCTAACAGTT CAAGAGCCTC CGAATTTTGT TGTCAAACCT 
CGTGACCAAG TTGTTGCTCT GGGACGCACA GTCACATTTC AGTGTGAAGC CACCGGAAAC 
CCCCAGCCAG CCATTTTTTG GCAAAAGGAG GGAAGCCAGA ACCTTCTCTT TTCCTACCAA 
CCTCCACAAT CGTCGAGCCG CTTCTCCGTG TCCCAGACTG GTGACCTGAC TATAACCACC 
GTTCAGAAGT CAGACGTGGG CTACTACATC TGCCAGACTC TCAACGTTGC CGGGAGCATT 
ACAACAAAAG CCTTCTTAGA AGTTACCGAT GTGGTGGCCG ACCGTCCTCC TCCAGTCATT 
CGTCAGGGAC CCGTGAATCA GACAGTAGCT GTTGATGGCA CCTTAATAAT GAACTGTATC 
GCCAGCGGCA TTCCTGTTCC CACAATCCAC TGGAGAAAGG ACGGGATCCT GATACCGACC 
CAAGACTCTC GCATTAAACA GCAGGACTCA GGAGCTCTGC AGATCCGTTA CGCCAAGCTG 
AGTGATACCG GTCGGTACAC CTGCACTGCC TCCACTACTA GCGGGGAAGC AAACTGGAGC 
GCGTACATAG AAGTCCAAGA ATTTGGAGTT CCGGTTCAAC CACCCAGAGC CACTGATCCC 
AACCTGATTC CAAGTGCTCC ATCAAAACCA GAAGTCACGG ACGTCAGCAA AAATACTGTA 
ACTTTATCCT GGCAGCCAAA CCTCAACTCA GGTGCTACTC CTACGTCGTA CCTCATAGAG 
GCCTTCAGCC ATGCGTCTGG AAGCAGCTGG CAAACAGTGG CAGAAAATGT CAAGACCGAT 
TCCTTTGCAG TTAAAGCACT GAAGCCCAAT GCAATCTACC TCTTCCTCGT GAGAGCAGTA 
AACGCATACG GATTAAGTGA TCCAAGCCAA ATATCCGATC CTGTAAAAAC ACAAGATATT 
CCTCCTACAA GTCAAGGAGT GGATCACAAA CAGGTGCAGA GAGAACTGGG CGATGTTGTG 
CTACACCTTC ATAACCCAAC AATACTTTCC TCTTCCTCCA TCGAAGTACA TTGGACTGTG 
GACCAGCAAT CACAGTACAT CCAAGGATAC AAGATCCTCT ACAGGCCAAG CGCAGAGAAT 
CGAGGCGAAT CTGCATGGTC CATATTTGAA GTCAGGACTC CGGCCAAAAA CAGCGCAGTT 
ATTCCTGAAC TGAGAAAAGG CGTTAACTAC GAGATCAAAG CCCGCCCCTT TTTTAATGAG 
TTTCAAGGAG CTGATAGTGA AGTGCGGTTC GCTAAGACAA TGGAAGAAGC ACCCAGTGCT 
CCCCCGCAGA GCGTTACTGT AACCAAGAAT GATGGGAATG GAACTGCTAT TGTTGTGGCA 
TGGCAGCCTC CCCCTGAAGA TACTCAGAAT GGGATGGTCC AGGAATACAA GGTTTGGTGC 
CTGGGGAATG AGAGTCGCTA TCACATAAAC AAGACTGTCG ATGGCTCTAC CCTTTCCGTG 
GTCATCCCGT CCTTAGTCCC TGGTATCAAA TACAGCGTGG AGGTGGCAGC CAGCACGGGC 
GCAGGATCCG GTGTGAAAAG TGAACCCAAC TACATCCAGT TAGATTCCTT TGGAAACCCG 
GTGCCTCCGG GGGACCAAGT CAGCCTGGCC CAGCAGATAT CCGATGTGGT GAAGCAGCCG 
GCATTCATAG CAGGGATTGG TGCTGCCTGC TGGATAATCC TCATGGTATT CAGTATCTGG 
TTGTACCGGC ACAGAAAGAA ACGCAATGGG CTCACCAGTT CCTATGCTGG AATACGGAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTG GCGTACCAGC GAGGAGGGGA AGCTGTAAGC 
AGCGCAGGCC GGCCTGGGCT CTTAAATATT TCTGAGCCTG CTAATCAGCC GTGGCTCGCA 
GATACCTGGC CAAACACTGG AAACAATCAC AACGAATGCC CCATCAACTG CTGCACCTCC 
GGGAACGGCA ACAGCGACAG CAACCTCGCC ACTTACAGCC GCCCAGCTGA CTGCATAGCG 
AATTACAACA ACCAGTTGGA CAGCAAGCAG ACAAACCTGA TGGTGCCCGA CTCGGCTGTT 
TATGGGGATG TAGACTTAAG CAACAAGATC AATGAGATGA AAACCTTCAA CAGCCCCAAT 
CTGAAAGATG GGAGGTTTGT CAATCCTGCC GGCCAGCCCA CCCCTTATGC CACCACTCAG 
CTCATCCAGT CCAACATAAG CAACAACATC AACACGTCTG CCGGTGATGT GGATGAAAAA 
CACTGGAAAC CCCCGTGCCA ACAGAAACCG GATGTTACCC CAATGCAGTA CAACATCATG 
GAGCAAAACA AGATCAACAA AGATTACCGA GCTTCGGACA CCATTCCTTC CACCATTCCC 
TACAACCAGT CCCATGATCA GAATGCCGGA GGGTCATACA ACAGCTCAGA CCGGGGAAGT 
AGCACATCTG GGAGCCAAGG TCATAAGAAG AGTACTCGTA CCCCGAAAGT ATCAAAGCAG 
GCTGGCATGA ACTGGGCAGA TCTTTTGCCA CCACCACCTG CTCACCCCCC ACCACATGGT 
GCCACTGAAG ACTACACTTT GTCAGCAGAT GAAAGCTATG ACAGAGAGTT ACCTTGCCCA 
ATACCACCTA CAAGAATGTA TCTTCAGCAA GACGAACTGG AAGAGGAAGA TGAAAGGGGT 
CCCACACCTC CTATTAGAGG AGCAGCTTCT TCTCCGGCTG CTGTGTCCTA CAGCCATCAG 
TCCACTGCCA CTCTTACCCC ATCACCTCAG GACGAACTCC AGCCAATGCT TCAGGACTGT 
CCCGAGGAGA TCGGTCATAC TCAGCATCAG CTAGAAAGAA GGCGTCATCA AATTAGTCCT 
CCTCCACCTC CAAGGCCCAT TTCCCCGCCT CATACATATG GTTATATTTC TGGACCAATA 
GTCTCCGATA TGGATACTGA TGTTCCTGAA GAGGAAGGGG ATGATGGGGA AATTGAAGTC 
ACCAAGATGC AAAGTCGCAG ACTTCTTTTA CGTGGCCTGG AGCAGACTCC TGCTTCCAGT 
GTTGGAGACC TAGAAAGTTC TGTGACTGGT TCCATGATCA ATGGATGGGG CTCAGCTTCT 
GAAGAAGACA ATGTTTCCAG TGGACGTTCC AGTGTCAGTT CATCGGATGG ATCATTTTTC 
ACGGATGCGG ATTTTGCACA GGCTGTCGCC GCAGCCGCAG AATATGCTGG GCTTAAAGTA 
GCAAGGCATC AAATGCAGGA AGCAGCAGGA GGTCGAAGAC CTTTCCACGC GTCTCACTGT 
CCAAGACCAA CAAGCCCAGT GTCAACAGAC AGCAACATGA GCACCGCTGT CATACAGAAA 
AGCCGTCCTG CCAAAAAACA AAAACACCAG CAGGGACATC TTCGGAAAGA TGTCTACACA 
GATGATTTGC CACCACCGCC ACTGCCACCT CCTGCTATCA AATCTCCAAC TGGTCCATCC 
AAAGCACAAC TAGAAGGACG GCCTGGCCTT TCACAGAAGT ACCTTGTTGC AGAACAAAGA 
CTGGAAAGGC CAGGAGAGAG GAAAGGTGTC AGCTACAGGG GCAGGGAAGG TCCTGACAGC 
AGACAACAAC ATGCAGAAGC AAGAACCAAT TCAGGGGAGA GAAGAGAGTC TTCGGAACAG 
CAACTTGATG GAAAGATGAG AAAAGGGAAA GCTTCAAAGT GGGATGGGGC ACCTATAGTA 
GCAAATCAAA TACAAGAGGA TATATTACCC TATTGCAAAC CAACATTCCC CACTTCAAAC 
AACCCTCGTG ATCCCAGTTC CTCCAGCTCA ATGTCCTCAA GAGGATCTGG TGGACGACGG 
AGGGGAGAAC AAACAACAGG TCGCAGAAAT GCAGCAGAGA TGCAGCTCTT AGGAAATTAT 
GAACGAGGGG TTGATGAAGA TGAAATAGAG GAAACAGAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	robo1
Entrez Gene ID	100124716
Full Name	roundabout guidance receptor 1
Synonyms	dutt1,roundabout,roundabout-1,sax3
General protein information	Preferred Names roundabout guidance receptor 1 Names roundabout homolog 1 roundabout, axon guidance receptor, homolog 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010242	XP_554155.3
Danio rerio (zebrafish)	robo1	NP_571556.1
Drosophila melanogaster (fruit fly)	robo3	NP_001259866.1
Homo sapiens (human)	ROBO1	NP_002932.1
Pan troglodytes (chimpanzee)	ROBO1	XP_516592.3
Gallus gallus (chicken)	ROBO1	XP_004938403.1
Caenorhabditis elegans (roundworm)	sax-3	NP_001024990.1
Xenopus tropicalis (tropical clawed frog)	robo1	NP_001096171.1
	ROBO1	XP_002802665.1
Canis lupus familiaris (dog)	ROBO1	XP_544814.4
Bos taurus (cattle)	ROBO1	NP_001179817.2
Mus musculus (house mouse)	Robo1	NP_062286.2
Rattus norvegicus (Norway rat)	Robo1	NP_071524.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

robo1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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