ROBO1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005201172.3	XP_005201229.1	roundabout homolog 1 isoform X8
NM_001192888.2	NP_001179817.2	roundabout homolog 1 precursor
XM_005201165.4	XP_005201222.1	roundabout homolog 1 isoform X1
XM_005201171.4	XP_005201228.1	roundabout homolog 1 isoform X5
XM_005201165.3	XP_005201222.1	roundabout homolog 1 isoform X1
XM_005201168.3	XP_005201225.1	roundabout homolog 1 isoform X3
XM_005201170.4	XP_005201227.1	roundabout homolog 1 isoform X4
XM_005201172.4	XP_005201229.1	roundabout homolog 1 isoform X7
XM_015468389.1	XP_015323875.1	roundabout homolog 1 isoform X2
XM_005201169.3	XP_005201226.1	roundabout homolog 1 isoform X5
XM_005201171.3	XP_005201228.1	roundabout homolog 1 isoform X7
XM_005201168.2	XP_005201225.1	roundabout homolog 1 isoform X4
XM_024990628.1	XP_024846396.1	roundabout homolog 1 isoform X6
XM_005201167.3	XP_005201224.1	roundabout homolog 1 isoform X3
XM_005201170.3	XP_005201227.1	roundabout homolog 1 isoform X6
XM_005201167.4	XP_005201224.1	roundabout homolog 1 isoform X2
NM_001192888.3	NP_001179817.2	roundabout homolog 1 precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa218842	XM_024990628.1 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa87635	XM_015468389.1 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa15492	NM_001192888.3 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15492	XM_005201165.4 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15494	XM_005201167.4 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15495	XM_005201168.3 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15496	XM_005201169.3 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15497	XM_005201170.4 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15498	XM_005201171.4 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15499	XM_005201172.4 Latest version!	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa15492	XM_005201165.3	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15499	XM_005201172.3	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15498	XM_005201171.3	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15495	XM_005201168.2	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15497	XM_005201170.3	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15494	XM_005201167.3	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa15492	NM_001192888.2	Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ROBO1 ( XM_024990628.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846396.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa218842
Clone ID Related Accession (Same CDS sequence)	XM_024990628.1
Accession Version	XM_024990628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG 
AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT 
GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG 
GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGG GCAGCCCACC 
CCTTATGCCA CCACCCAGCT CATCCAGTCA AACCTCAGCA ACAACATGAA CAATGGCAGT 
GGAGACTCTG GCGAGAAGCA CTGGAAGCCA CCCGGACAGC AGAAACAGGA AGTGGCACCA 
ATTCAGTATA ACATCATGGA GCAAAACAAA CTGAACAAAG ATTATCGTGC AAATGACACA 
ATTGCTCCAA CTATCCCTTA CAACCAGTCA TATGACCAGA ATACTGGAGG ATCCTACAAC 
AGTTCAGACC GGGGAAGTAG TACATCAGGG AGTCAAGGGC ACAAGAAAGG GGCAAGGACA 
CCCAAAGTGC CAAAGCAAGG TGGCATGAAC TGGGCAGACC TACTGCCCCC TCCCCCAGCA 
CATCCTCCTC CTCATAGCAA CAGTGAAGAG TACAGCATCT CTGTAGATGA AAGCTATGAC 
CAAGAAATGC CATGCCCCGT GCCCCCAGCA CGGATGTATC TGCAACAAGG TGAACTAGAA 
GAGGAGGAAG ATGAACGAGG TCCCACTCCC CCTGTGCGGG GAGCCGCCTC TTCTCCGGCT 
GCCGTGTCTT ACAGCCATCA ATCCACCGCC ACTCTCACGC CCTCTCCACA GGAAGAACTC 
CAGCCCATGT TACAGGATTG TCCCGAGGAG GTGGGCCATG GGCAGCACCA GCCTGACAGG 
AGACGGCAGC CGGTGAGCCC ACCTCCACCT CCAAGGCCGA TTTCCCCACC CCACACCTAC 
GGCTACATTT CAGGACCCCT TGTCTCAGAT ATGGATACGG ATGCGCCAGA AGAGGAAGAA 
GACGAAGCCG ACATGGAAGT AGCCAAGATG CAGACCAGGA GGCTTTTGTT ACGTGGGCTG 
GAGCAGACCC CTGCCTCCAG TGTCGGGGAC CTGGAGAGCT CTGTCACCGG GTCCATGATC 
AATGGTTGGG GCTCTGCCTC AGAGGAGGAC AACATTTCCA GCGGACGCTC CAGCGTTAGT 
TCTTCTGATG GCTCTTTTTT CACTGATGCC GATTTTGCCC AGGCGGTCGC AGCAGCAGCG 
GAGTATGCTG GTCTGAAAGT GGCCCGGCGC CAAATGCAGG ATGCTGCTGG CCGCCGCCAT 
TTTCATGCAT CCCAGTGCCC TAGACCCACA AGTCCTGTTT CTACAGACAG CAACGTGAGT 
GCTGCTGTAG TACAGAAGGC CAGACCAGCC AAGAAACAAA AACACCAGCC AGGACATCTG 
CGCAGAGAAG CCTACACAGA TGATCTTCCA CCTCCCCCTG TGCCACCACC TGCTATCAAG 
TCACCCACTG CCCAGTCCAA GGCCCAGCTG GAGGTTCGAC CCGTGATGGT GCCAAAACTC 
CCTTCTATAG AAGCAAGAAC AGACAGATCA TCAGATAGGA AAGGAGGCAA TTACAAGGGG 
AGAGAAGTAT TGGATGGAAG ACAAGTTGCT GATATGCGAC CAAATCCAGG TGATGCCAGA 
GAAGCACAGG AACAGCCAAA TGACGGGAAA GGACGAGGAA ACAAGGCAGC AAAACGAGAC 
CTTCCACCAG CAAAGGCTCA TCTCATCCAA GAGGATATTC TGCCTTACTG TAGACCTACT 
TTTCCGACAT CAAATAATCC CAGAGATCCT AGTTCTTCAA GTTCTATGTC ATCAAGAGGA 
TCAGGAAGCA GACAAAGAGA ACAAGCAAAT GTAGGTCGAA GAAATATTGC AGAGATGCAA 
GTTCTTGGAG GATATGAAAG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846396.1
CDS	1112..5791
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_024990628.1
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990628.1

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG 
AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT 
GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG 
GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGG GCAGCCCACC 
CCTTATGCCA CCACCCAGCT CATCCAGTCA AACCTCAGCA ACAACATGAA CAATGGCAGT 
GGAGACTCTG GCGAGAAGCA CTGGAAGCCA CCCGGACAGC AGAAACAGGA AGTGGCACCA 
ATTCAGTATA ACATCATGGA GCAAAACAAA CTGAACAAAG ATTATCGTGC AAATGACACA 
ATTGCTCCAA CTATCCCTTA CAACCAGTCA TATGACCAGA ATACTGGAGG ATCCTACAAC 
AGTTCAGACC GGGGAAGTAG TACATCAGGG AGTCAAGGGC ACAAGAAAGG GGCAAGGACA 
CCCAAAGTGC CAAAGCAAGG TGGCATGAAC TGGGCAGACC TACTGCCCCC TCCCCCAGCA 
CATCCTCCTC CTCATAGCAA CAGTGAAGAG TACAGCATCT CTGTAGATGA AAGCTATGAC 
CAAGAAATGC CATGCCCCGT GCCCCCAGCA CGGATGTATC TGCAACAAGG TGAACTAGAA 
GAGGAGGAAG ATGAACGAGG TCCCACTCCC CCTGTGCGGG GAGCCGCCTC TTCTCCGGCT 
GCCGTGTCTT ACAGCCATCA ATCCACCGCC ACTCTCACGC CCTCTCCACA GGAAGAACTC 
CAGCCCATGT TACAGGATTG TCCCGAGGAG GTGGGCCATG GGCAGCACCA GCCTGACAGG 
AGACGGCAGC CGGTGAGCCC ACCTCCACCT CCAAGGCCGA TTTCCCCACC CCACACCTAC 
GGCTACATTT CAGGACCCCT TGTCTCAGAT ATGGATACGG ATGCGCCAGA AGAGGAAGAA 
GACGAAGCCG ACATGGAAGT AGCCAAGATG CAGACCAGGA GGCTTTTGTT ACGTGGGCTG 
GAGCAGACCC CTGCCTCCAG TGTCGGGGAC CTGGAGAGCT CTGTCACCGG GTCCATGATC 
AATGGTTGGG GCTCTGCCTC AGAGGAGGAC AACATTTCCA GCGGACGCTC CAGCGTTAGT 
TCTTCTGATG GCTCTTTTTT CACTGATGCC GATTTTGCCC AGGCGGTCGC AGCAGCAGCG 
GAGTATGCTG GTCTGAAAGT GGCCCGGCGC CAAATGCAGG ATGCTGCTGG CCGCCGCCAT 
TTTCATGCAT CCCAGTGCCC TAGACCCACA AGTCCTGTTT CTACAGACAG CAACGTGAGT 
GCTGCTGTAG TACAGAAGGC CAGACCAGCC AAGAAACAAA AACACCAGCC AGGACATCTG 
CGCAGAGAAG CCTACACAGA TGATCTTCCA CCTCCCCCTG TGCCACCACC TGCTATCAAG 
TCACCCACTG CCCAGTCCAA GGCCCAGCTG GAGGTTCGAC CCGTGATGGT GCCAAAACTC 
CCTTCTATAG AAGCAAGAAC AGACAGATCA TCAGATAGGA AAGGAGGCAA TTACAAGGGG 
AGAGAAGTAT TGGATGGAAG ACAAGTTGCT GATATGCGAC CAAATCCAGG TGATGCCAGA 
GAAGCACAGG AACAGCCAAA TGACGGGAAA GGACGAGGAA ACAAGGCAGC AAAACGAGAC 
CTTCCACCAG CAAAGGCTCA TCTCATCCAA GAGGATATTC TGCCTTACTG TAGACCTACT 
TTTCCGACAT CAAATAATCC CAGAGATCCT AGTTCTTCAA GTTCTATGTC ATCAAGAGGA 
TCAGGAAGCA GACAAAGAGA ACAAGCAAAT GTAGGTCGAA GAAATATTGC AGAGATGCAA 
GTTCTTGGAG GATATGAAAG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_015468389.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323875.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa87635
Clone ID Related Accession (Same CDS sequence)	XM_015468389.1
Accession Version	XM_015468389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTGGTTGCCT TGGGACGAAC GGTCACTTTC CAGTGTGAAG CAACAGGAAA TCCCCAACCA 
GCTATCTTCT GGAGGAGAGA GGGGAGTCAG AATCTGCTTT TCTCATATCA GCCACCACAG 
TCATCCAGCC GTTTTTCAGT CTCCCAGACT GGTGACCTCA CTATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTATAT CTGCCAGACT TTGAATGTTG CCGGAAGTAT CATCACAAAA 
GCATATTTGG AAGTTACCGA TGTGATTGCA GACCGACCTC CCCCAGTCAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACAATTCT GTGGAGAAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT 
CGAATCAAAC AGCTGGAGAC CGGAGTACTA CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGATACA CGTGCATTGC ATCAACCCCC AGTGGTGAAG CAACGTGGAG TGCTTACATT 
GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CCACCGACCC AAATTTAATC 
CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCC GAAATACAGT AACGTTGTCA 
TGGCAACCAA ATTTGAATTC AGGTGCAACT CCGACGTCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACTGTA GCGGAGAATG TGAAAACAGA AACATTTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC CAATGCATAT 
GGAATTAGTG ATCCAAGCCA GATATCAGAC CCAGTGAAAA CACAAGATGT TCCCCCAACC 
AGTCAGGGGG TGGATCACAA GCAGGTCCAG CGAGAACTGG GAAATGTTGT ACTGCATCTC 
CACAACCCCA CCATCCTTTC CTCCTCTTCA ATCGAAGTGC ACTGGACAGT GGACCAACAG 
TCTCAGTATA TTCAAGGATA CAAAGTTCTT TACCGCCCGT CTGGAGCCAA CCATGGTGAA 
TCCGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ACAGTGTGGT TATCCCTGAT 
CTCAAGAAAG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATTAAATT TGCCAAAACC CTAGAAGAAG CACCCAGTGC CCCACCTCAA 
AGTGTGACTG TATCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGCCA 
CCTCCTGAAG ATACTCAAAA TGGAATGGTC CAAGAGTATA AGGTCTGGTG TCTGGGCAAT 
GAAACGCGAT ACCATATAAA CAAGACAGTA GATGGTTCCA CCTTTTCTGT GGTTATTCCC 
TCACTGGTTC CTGGGATCCG ATATAGTGTA GAGGTGGCAG CCAGCACGGG GGCTGGGTCG 
GGGGTGAAGA GTGAGCCACA ATTCATCCAG TTAGATTCTC ATGGAAACCC CGTGTCACCT 
GAGGACCAAG TCAGCCTTGC TCAGCAGATC TCAGACGTAG TGAAGCAGCC GGCATTCATC 
GCAGGAATCG GGGCTGCCTG TTGGATCATC CTTATGGTTT TTAGCATCTG GCTCTATCGA 
CACCGAAAGA AGAGAAATGG GCTGGCTAGT ACTTATGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGAGGCG AAGCTGTGAG CAGTGGAGGG 
AGGCCAGGAC TTCTCAACAT CAGTGAACCT GCCACACAGC CATGGCTTGC AGACACATGG 
CCTAACACTG GCAACAATCA CAATGACTGC TCCATCAATT GCTGCACGGC AGGCAATGGG 
AACAGTGACA GCAACTTGAC CACGTATAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG 
CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG 
TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA 
GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG 
AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA 
GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA 
GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT 
CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC 
GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG 
GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA 
CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG 
ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG 
GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT 
GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG 
CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA 
GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG 
CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA 
TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC 
CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323875.1
CDS	297..5249
Translation	MKWTHAPFLAIISLLSLSKSHLFLAQLIPDPEDVERGKDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_015468389.1
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468389.1

ORF Insert Sequence:

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTGGTTGCCT TGGGACGAAC GGTCACTTTC CAGTGTGAAG CAACAGGAAA TCCCCAACCA 
GCTATCTTCT GGAGGAGAGA GGGGAGTCAG AATCTGCTTT TCTCATATCA GCCACCACAG 
TCATCCAGCC GTTTTTCAGT CTCCCAGACT GGTGACCTCA CTATTACTAA TGTCCAGCGA 
TCTGATGTTG GTTATTATAT CTGCCAGACT TTGAATGTTG CCGGAAGTAT CATCACAAAA 
GCATATTTGG AAGTTACCGA TGTGATTGCA GACCGACCTC CCCCAGTCAT TCGACAAGGT 
CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC 
AGTCCAGTGC CCACAATTCT GTGGAGAAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT 
CGAATCAAAC AGCTGGAGAC CGGAGTACTA CAGATCCGAT ATGCTAAGCT GGGTGATACT 
GGTCGATACA CGTGCATTGC ATCAACCCCC AGTGGTGAAG CAACGTGGAG TGCTTACATT 
GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CCACCGACCC AAATTTAATC 
CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCC GAAATACAGT AACGTTGTCA 
TGGCAACCAA ATTTGAATTC AGGTGCAACT CCGACGTCTT ATATTATAGA AGCCTTCAGC 
CATGCATCTG GTAGCAGCTG GCAGACTGTA GCGGAGAATG TGAAAACAGA AACATTTGCC 
ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC CAATGCATAT 
GGAATTAGTG ATCCAAGCCA GATATCAGAC CCAGTGAAAA CACAAGATGT TCCCCCAACC 
AGTCAGGGGG TGGATCACAA GCAGGTCCAG CGAGAACTGG GAAATGTTGT ACTGCATCTC 
CACAACCCCA CCATCCTTTC CTCCTCTTCA ATCGAAGTGC ACTGGACAGT GGACCAACAG 
TCTCAGTATA TTCAAGGATA CAAAGTTCTT TACCGCCCGT CTGGAGCCAA CCATGGTGAA 
TCCGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ACAGTGTGGT TATCCCTGAT 
CTCAAGAAAG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATTAAATT TGCCAAAACC CTAGAAGAAG CACCCAGTGC CCCACCTCAA 
AGTGTGACTG TATCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGCCA 
CCTCCTGAAG ATACTCAAAA TGGAATGGTC CAAGAGTATA AGGTCTGGTG TCTGGGCAAT 
GAAACGCGAT ACCATATAAA CAAGACAGTA GATGGTTCCA CCTTTTCTGT GGTTATTCCC 
TCACTGGTTC CTGGGATCCG ATATAGTGTA GAGGTGGCAG CCAGCACGGG GGCTGGGTCG 
GGGGTGAAGA GTGAGCCACA ATTCATCCAG TTAGATTCTC ATGGAAACCC CGTGTCACCT 
GAGGACCAAG TCAGCCTTGC TCAGCAGATC TCAGACGTAG TGAAGCAGCC GGCATTCATC 
GCAGGAATCG GGGCTGCCTG TTGGATCATC CTTATGGTTT TTAGCATCTG GCTCTATCGA 
CACCGAAAGA AGAGAAATGG GCTGGCTAGT ACTTATGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGAGGCG AAGCTGTGAG CAGTGGAGGG 
AGGCCAGGAC TTCTCAACAT CAGTGAACCT GCCACACAGC CATGGCTTGC AGACACATGG 
CCTAACACTG GCAACAATCA CAATGACTGC TCCATCAATT GCTGCACGGC AGGCAATGGG 
AACAGTGACA GCAACTTGAC CACGTATAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT 
GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG 
CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG 
TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA 
GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG 
AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA 
GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA 
GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT 
CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC 
GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG 
GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA 
CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG 
ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG 
GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT 
GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG 
CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA 
GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG 
CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA 
TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC 
CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( NM_001192888.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179817.2 Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA.

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CloneID	OBa15492
Clone ID Related Accession (Same CDS sequence)	NM_001192888.2 , XM_005201165.4 , XM_005201165.3 , NM_001192888.3
Accession Version	NM_001192888.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02000642.1, DAAA02000605.1, DAAA02000603.1, DAAA02000593.1, DAAA02000592.1 and DAAA02000591.1. On Apr 26, 2011 this sequence version replaced NM_001192888.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822095, SAMN04019640 [ECO:0000348] ##Evidence-Data-END##

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG 
CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT 
TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT 
AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA 
GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC 
AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC 
GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA 
GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT 
CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC 
CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC 
CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA 
GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC 
AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT 
TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA 
GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC 
CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG 
GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC 
AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA 
ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA 
AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT 
CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA 
AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179817.2
CDS	52..5013
Translation	MKWTHAPFLAIISLLSLSKSHLFLAQLIPDPEDVERGKDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	NM_001192888.2
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192888.2

ORF Insert Sequence:

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG 
CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT 
TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT 
AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA 
GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC 
AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC 
GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA 
GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT 
CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC 
CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC 
CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA 
GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC 
AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT 
TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA 
GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC 
CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG 
GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC 
AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA 
ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA 
AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT 
CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA 
AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( NM_001192888.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179817.2 Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA.

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CloneID	OBa15492
Clone ID Related Accession (Same CDS sequence)	NM_001192888.2 , XM_005201165.4 , XM_005201165.3 , NM_001192888.3
Accession Version	NM_001192888.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-15
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Jun 16, 2018 this sequence version replaced NM_001192888.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG 
CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT 
TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT 
AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA 
GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC 
AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC 
GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA 
GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT 
CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC 
CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC 
CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA 
GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC 
AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT 
TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA 
GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC 
CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG 
GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC 
AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA 
ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA 
AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT 
CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA 
AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179817.2
CDS	52..5013
Translation	MKWTHAPFLAIISLLSLSKSHLFLAQLIPDPEDVERGKDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	NM_001192888.3
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192888.3

ORF Insert Sequence:

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG 
CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT 
TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT 
AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA 
GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC 
AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC 
GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA 
GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT 
CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC 
CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC 
CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA 
GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC 
AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT 
TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA 
GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC 
CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG 
GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC 
AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA 
ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA 
AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT 
CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA 
AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201165.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201222.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

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CloneID	OBa15492
Clone ID Related Accession (Same CDS sequence)	NM_001192888.2 , XM_005201165.4 , XM_005201165.3 , NM_001192888.3
Accession Version	XM_005201165.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201165.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG 
CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT 
TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT 
AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA 
GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC 
AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC 
GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA 
GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT 
CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC 
CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC 
CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA 
GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC 
AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT 
TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA 
GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC 
CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG 
GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC 
AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA 
ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA 
AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT 
CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA 
AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201222.1
CDS	297..5258
Translation	MKWTHAPFLAIISLLSLSKSHLFLAQLIPDPEDVERGKDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201165.4
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201165.4

ORF Insert Sequence:

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG 
CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT 
TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT 
AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA 
GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC 
AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC 
GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA 
GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT 
CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC 
CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC 
CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA 
GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC 
AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT 
TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA 
GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC 
CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG 
GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC 
AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA 
ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA 
AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT 
CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA 
AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201165.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201222.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

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CloneID	OBa15492
Clone ID Related Accession (Same CDS sequence)	NM_001192888.2 , XM_005201165.4 , XM_005201165.3 , NM_001192888.3
Accession Version	XM_005201165.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG 
CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT 
TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT 
AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA 
GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC 
AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC 
GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA 
GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT 
CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC 
CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC 
CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA 
GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC 
AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT 
TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA 
GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC 
CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG 
GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC 
AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA 
ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA 
AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT 
CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA 
AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201222.1
CDS	297..5258
Translation	MKWTHAPFLAIISLLSLSKSHLFLAQLIPDPEDVERGKDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201165.3
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201165.3

ORF Insert Sequence:

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG 
CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG 
CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT 
TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT 
AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA 
GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC 
AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC 
GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA 
GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT 
CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC 
CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC 
CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA 
GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC 
AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC 
TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT 
TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA 
GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC 
CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG 
GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC 
AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA 
ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA 
AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT 
CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA 
AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201167.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201224.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa15494
Clone ID Related Accession (Same CDS sequence)	XM_005201167.3 , XM_005201167.4
Accession Version	XM_005201167.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201167.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTAACTTATC AGAGAGGAGG CGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACACA GCCATGGCTT GCAGACACAT GGCCTAACAC TGGCAACAAT 
CACAATGACT GCTCCATCAA TTGCTGCACG GCAGGCAATG GGAACAGTGA CAGCAACTTG 
ACCACGTATA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CCACCCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGTGGAGA CTCTGGCGAG AAGCACTGGA AGCCACCCGG ACAGCAGAAA 
CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT 
CGTGCAAATG ACACAATTGC TCCAACTATC CCTTACAACC AGTCATATGA CCAGAATACT 
GGAGGATCCT ACAACAGTTC AGACCGGGGA AGTAGTACAT CAGGGAGTCA AGGGCACAAG 
AAAGGGGCAA GGACACCCAA AGTGCCAAAG CAAGGTGGCA TGAACTGGGC AGACCTACTG 
CCCCCTCCCC CAGCACATCC TCCTCCTCAT AGCAACAGTG AAGAGTACAG CATCTCTGTA 
GATGAAAGCT ATGACCAAGA AATGCCATGC CCCGTGCCCC CAGCACGGAT GTATCTGCAA 
CAAGGTGAAC TAGAAGAGGA GGAAGATGAA CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC 
GCCTCTTCTC CGGCTGCCGT GTCTTACAGC CATCAATCCA CCGCCACTCT CACGCCCTCT 
CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCCG AGGAGGTGGG CCATGGGCAG 
CACCAGCCTG ACAGGAGACG GCAGCCGGTG AGCCCACCTC CACCTCCAAG GCCGATTTCC 
CCACCCCACA CCTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACGGATGCG 
CCAGAAGAGG AAGAAGACGA AGCCGACATG GAAGTAGCCA AGATGCAGAC CAGGAGGCTT 
TTGTTACGTG GGCTGGAGCA GACCCCTGCC TCCAGTGTCG GGGACCTGGA GAGCTCTGTC 
ACCGGGTCCA TGATCAATGG TTGGGGCTCT GCCTCAGAGG AGGACAACAT TTCCAGCGGA 
CGCTCCAGCG TTAGTTCTTC TGATGGCTCT TTTTTCACTG ATGCCGATTT TGCCCAGGCG 
GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG AAAGTGGCCC GGCGCCAAAT GCAGGATGCT 
GCTGGCCGCC GCCATTTTCA TGCATCCCAG TGCCCTAGAC CCACAAGTCC TGTTTCTACA 
GACAGCAACG TGAGTGCTGC TGTAGTACAG AAGGCCAGAC CAGCCAAGAA ACAAAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC CCCTGTGCCA 
CCACCTGCTA TCAAGTCACC CACTGCCCAG TCCAAGGCCC AGCTGGAGGT TCGACCCGTG 
ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACA GATCATCAGA TAGGAAAGGA 
GGCAATTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCTGATAT GCGACCAAAT 
CCAGGTGATG CCAGAGAAGC ACAGGAACAG CCAAATGACG GGAAAGGACG AGGAAACAAG 
GCAGCAAAAC GAGACCTTCC ACCAGCAAAG GCTCATCTCA TCCAAGAGGA TATTCTGCCT 
TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGTTCT 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAGA TGCAAGTTCT TGGAGGATAT GAAAGAGAAG ATAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201224.1
CDS	297..5231
Translation	MKWTHAPFLAIISLLSLSKSHLFLAQLIPDPEDVERGKDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201167.3
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201167.3

ORF Insert Sequence:

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTAACTTATC AGAGAGGAGG CGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACACA GCCATGGCTT GCAGACACAT GGCCTAACAC TGGCAACAAT 
CACAATGACT GCTCCATCAA TTGCTGCACG GCAGGCAATG GGAACAGTGA CAGCAACTTG 
ACCACGTATA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CCACCCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGTGGAGA CTCTGGCGAG AAGCACTGGA AGCCACCCGG ACAGCAGAAA 
CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT 
CGTGCAAATG ACACAATTGC TCCAACTATC CCTTACAACC AGTCATATGA CCAGAATACT 
GGAGGATCCT ACAACAGTTC AGACCGGGGA AGTAGTACAT CAGGGAGTCA AGGGCACAAG 
AAAGGGGCAA GGACACCCAA AGTGCCAAAG CAAGGTGGCA TGAACTGGGC AGACCTACTG 
CCCCCTCCCC CAGCACATCC TCCTCCTCAT AGCAACAGTG AAGAGTACAG CATCTCTGTA 
GATGAAAGCT ATGACCAAGA AATGCCATGC CCCGTGCCCC CAGCACGGAT GTATCTGCAA 
CAAGGTGAAC TAGAAGAGGA GGAAGATGAA CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC 
GCCTCTTCTC CGGCTGCCGT GTCTTACAGC CATCAATCCA CCGCCACTCT CACGCCCTCT 
CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCCG AGGAGGTGGG CCATGGGCAG 
CACCAGCCTG ACAGGAGACG GCAGCCGGTG AGCCCACCTC CACCTCCAAG GCCGATTTCC 
CCACCCCACA CCTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACGGATGCG 
CCAGAAGAGG AAGAAGACGA AGCCGACATG GAAGTAGCCA AGATGCAGAC CAGGAGGCTT 
TTGTTACGTG GGCTGGAGCA GACCCCTGCC TCCAGTGTCG GGGACCTGGA GAGCTCTGTC 
ACCGGGTCCA TGATCAATGG TTGGGGCTCT GCCTCAGAGG AGGACAACAT TTCCAGCGGA 
CGCTCCAGCG TTAGTTCTTC TGATGGCTCT TTTTTCACTG ATGCCGATTT TGCCCAGGCG 
GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG AAAGTGGCCC GGCGCCAAAT GCAGGATGCT 
GCTGGCCGCC GCCATTTTCA TGCATCCCAG TGCCCTAGAC CCACAAGTCC TGTTTCTACA 
GACAGCAACG TGAGTGCTGC TGTAGTACAG AAGGCCAGAC CAGCCAAGAA ACAAAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC CCCTGTGCCA 
CCACCTGCTA TCAAGTCACC CACTGCCCAG TCCAAGGCCC AGCTGGAGGT TCGACCCGTG 
ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACA GATCATCAGA TAGGAAAGGA 
GGCAATTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCTGATAT GCGACCAAAT 
CCAGGTGATG CCAGAGAAGC ACAGGAACAG CCAAATGACG GGAAAGGACG AGGAAACAAG 
GCAGCAAAAC GAGACCTTCC ACCAGCAAAG GCTCATCTCA TCCAAGAGGA TATTCTGCCT 
TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGTTCT 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAGA TGCAAGTTCT TGGAGGATAT GAAAGAGAAG ATAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201167.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201224.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa15494
Clone ID Related Accession (Same CDS sequence)	XM_005201167.3 , XM_005201167.4
Accession Version	XM_005201167.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201167.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTAACTTATC AGAGAGGAGG CGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACACA GCCATGGCTT GCAGACACAT GGCCTAACAC TGGCAACAAT 
CACAATGACT GCTCCATCAA TTGCTGCACG GCAGGCAATG GGAACAGTGA CAGCAACTTG 
ACCACGTATA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CCACCCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGTGGAGA CTCTGGCGAG AAGCACTGGA AGCCACCCGG ACAGCAGAAA 
CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT 
CGTGCAAATG ACACAATTGC TCCAACTATC CCTTACAACC AGTCATATGA CCAGAATACT 
GGAGGATCCT ACAACAGTTC AGACCGGGGA AGTAGTACAT CAGGGAGTCA AGGGCACAAG 
AAAGGGGCAA GGACACCCAA AGTGCCAAAG CAAGGTGGCA TGAACTGGGC AGACCTACTG 
CCCCCTCCCC CAGCACATCC TCCTCCTCAT AGCAACAGTG AAGAGTACAG CATCTCTGTA 
GATGAAAGCT ATGACCAAGA AATGCCATGC CCCGTGCCCC CAGCACGGAT GTATCTGCAA 
CAAGGTGAAC TAGAAGAGGA GGAAGATGAA CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC 
GCCTCTTCTC CGGCTGCCGT GTCTTACAGC CATCAATCCA CCGCCACTCT CACGCCCTCT 
CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCCG AGGAGGTGGG CCATGGGCAG 
CACCAGCCTG ACAGGAGACG GCAGCCGGTG AGCCCACCTC CACCTCCAAG GCCGATTTCC 
CCACCCCACA CCTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACGGATGCG 
CCAGAAGAGG AAGAAGACGA AGCCGACATG GAAGTAGCCA AGATGCAGAC CAGGAGGCTT 
TTGTTACGTG GGCTGGAGCA GACCCCTGCC TCCAGTGTCG GGGACCTGGA GAGCTCTGTC 
ACCGGGTCCA TGATCAATGG TTGGGGCTCT GCCTCAGAGG AGGACAACAT TTCCAGCGGA 
CGCTCCAGCG TTAGTTCTTC TGATGGCTCT TTTTTCACTG ATGCCGATTT TGCCCAGGCG 
GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG AAAGTGGCCC GGCGCCAAAT GCAGGATGCT 
GCTGGCCGCC GCCATTTTCA TGCATCCCAG TGCCCTAGAC CCACAAGTCC TGTTTCTACA 
GACAGCAACG TGAGTGCTGC TGTAGTACAG AAGGCCAGAC CAGCCAAGAA ACAAAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC CCCTGTGCCA 
CCACCTGCTA TCAAGTCACC CACTGCCCAG TCCAAGGCCC AGCTGGAGGT TCGACCCGTG 
ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACA GATCATCAGA TAGGAAAGGA 
GGCAATTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCTGATAT GCGACCAAAT 
CCAGGTGATG CCAGAGAAGC ACAGGAACAG CCAAATGACG GGAAAGGACG AGGAAACAAG 
GCAGCAAAAC GAGACCTTCC ACCAGCAAAG GCTCATCTCA TCCAAGAGGA TATTCTGCCT 
TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGTTCT 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAGA TGCAAGTTCT TGGAGGATAT GAAAGAGAAG ATAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201224.1
CDS	297..5231
Translation	MKWTHAPFLAIISLLSLSKSHLFLAQLIPDPEDVERGKDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201167.4
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201167.4

ORF Insert Sequence:

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTAACTTATC AGAGAGGAGG CGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC 
ATCAGTGAAC CTGCCACACA GCCATGGCTT GCAGACACAT GGCCTAACAC TGGCAACAAT 
CACAATGACT GCTCCATCAA TTGCTGCACG GCAGGCAATG GGAACAGTGA CAGCAACTTG 
ACCACGTATA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CCACCCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC 
ATGAACAATG GCAGTGGAGA CTCTGGCGAG AAGCACTGGA AGCCACCCGG ACAGCAGAAA 
CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT 
CGTGCAAATG ACACAATTGC TCCAACTATC CCTTACAACC AGTCATATGA CCAGAATACT 
GGAGGATCCT ACAACAGTTC AGACCGGGGA AGTAGTACAT CAGGGAGTCA AGGGCACAAG 
AAAGGGGCAA GGACACCCAA AGTGCCAAAG CAAGGTGGCA TGAACTGGGC AGACCTACTG 
CCCCCTCCCC CAGCACATCC TCCTCCTCAT AGCAACAGTG AAGAGTACAG CATCTCTGTA 
GATGAAAGCT ATGACCAAGA AATGCCATGC CCCGTGCCCC CAGCACGGAT GTATCTGCAA 
CAAGGTGAAC TAGAAGAGGA GGAAGATGAA CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC 
GCCTCTTCTC CGGCTGCCGT GTCTTACAGC CATCAATCCA CCGCCACTCT CACGCCCTCT 
CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCCG AGGAGGTGGG CCATGGGCAG 
CACCAGCCTG ACAGGAGACG GCAGCCGGTG AGCCCACCTC CACCTCCAAG GCCGATTTCC 
CCACCCCACA CCTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACGGATGCG 
CCAGAAGAGG AAGAAGACGA AGCCGACATG GAAGTAGCCA AGATGCAGAC CAGGAGGCTT 
TTGTTACGTG GGCTGGAGCA GACCCCTGCC TCCAGTGTCG GGGACCTGGA GAGCTCTGTC 
ACCGGGTCCA TGATCAATGG TTGGGGCTCT GCCTCAGAGG AGGACAACAT TTCCAGCGGA 
CGCTCCAGCG TTAGTTCTTC TGATGGCTCT TTTTTCACTG ATGCCGATTT TGCCCAGGCG 
GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG AAAGTGGCCC GGCGCCAAAT GCAGGATGCT 
GCTGGCCGCC GCCATTTTCA TGCATCCCAG TGCCCTAGAC CCACAAGTCC TGTTTCTACA 
GACAGCAACG TGAGTGCTGC TGTAGTACAG AAGGCCAGAC CAGCCAAGAA ACAAAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC CCCTGTGCCA 
CCACCTGCTA TCAAGTCACC CACTGCCCAG TCCAAGGCCC AGCTGGAGGT TCGACCCGTG 
ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACA GATCATCAGA TAGGAAAGGA 
GGCAATTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCTGATAT GCGACCAAAT 
CCAGGTGATG CCAGAGAAGC ACAGGAACAG CCAAATGACG GGAAAGGACG AGGAAACAAG 
GCAGCAAAAC GAGACCTTCC ACCAGCAAAG GCTCATCTCA TCCAAGAGGA TATTCTGCCT 
TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGTTCT 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAGA TGCAAGTTCT TGGAGGATAT GAAAGAGAAG ATAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201168.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201225.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

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CloneID	OBa15495
Clone ID Related Accession (Same CDS sequence)	XM_005201168.3 , XM_005201168.2
Accession Version	XM_005201168.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201168.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG 
AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT 
GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG 
GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACCCCTTA TGCCACCACC 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGTGGAGA CTCTGGCGAG 
AAGCACTGGA AGCCACCCGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGTGCAAATG ACACAATTGC TCCAACTATC 
CCTTACAACC AGTCATATGA CCAGAATACT GGAGGATCCT ACAACAGTTC AGACCGGGGA 
AGTAGTACAT CAGGGAGTCA AGGGCACAAG AAAGGGGCAA GGACACCCAA AGTGCCAAAG 
CAAGGTGGCA TGAACTGGGC AGACCTACTG CCCCCTCCCC CAGCACATCC TCCTCCTCAT 
AGCAACAGTG AAGAGTACAG CATCTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGC 
CCCGTGCCCC CAGCACGGAT GTATCTGCAA CAAGGTGAAC TAGAAGAGGA GGAAGATGAA 
CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC GCCTCTTCTC CGGCTGCCGT GTCTTACAGC 
CATCAATCCA CCGCCACTCT CACGCCCTCT CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCCG AGGAGGTGGG CCATGGGCAG CACCAGCCTG ACAGGAGACG GCAGCCGGTG 
AGCCCACCTC CACCTCCAAG GCCGATTTCC CCACCCCACA CCTACGGCTA CATTTCAGGA 
CCCCTTGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAAGTAGCCA AGATGCAGAC CAGGAGGCTT TTGTTACGTG GGCTGGAGCA GACCCCTGCC 
TCCAGTGTCG GGGACCTGGA GAGCTCTGTC ACCGGGTCCA TGATCAATGG TTGGGGCTCT 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGCG TTAGTTCTTC TGATGGCTCT 
TTTTTCACTG ATGCCGATTT TGCCCAGGCG GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG 
AAAGTGGCCC GGCGCCAAAT GCAGGATGCT GCTGGCCGCC GCCATTTTCA TGCATCCCAG 
TGCCCTAGAC CCACAAGTCC TGTTTCTACA GACAGCAACG TGAGTGCTGC TGTAGTACAG 
AAGGCCAGAC CAGCCAAGAA ACAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC 
ACAGATGATC TTCCACCTCC CCCTGTGCCA CCACCTGCTA TCAAGTCACC CACTGCCCAG 
TCCAAGGCCC AGCTGGAGGT TCGACCCGTG ATGGTGCCAA AACTCCCTTC TATAGAAGCA 
AGAACAGACA GATCATCAGA TAGGAAAGGA GGCAATTACA AGGGGAGAGA AGTATTGGAT 
GGAAGACAAG TTGCTGATAT GCGACCAAAT CCAGGTGATG CCAGAGAAGC ACAGGAACAG 
CCAAATGACG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
GCTCATCTCA TCCAAGAGGA TATTCTGCCT TACTGTAGAC CTACTTTTCC GACATCAAAT 
AATCCCAGAG ATCCTAGTTC TTCAAGTTCT ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAGA TGCAAGTTCT TGGAGGATAT 
GAAAGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201225.1
CDS	1138..5982
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201168.3
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201168.3

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG 
AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT 
GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG 
GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACCCCTTA TGCCACCACC 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGTGGAGA CTCTGGCGAG 
AAGCACTGGA AGCCACCCGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGTGCAAATG ACACAATTGC TCCAACTATC 
CCTTACAACC AGTCATATGA CCAGAATACT GGAGGATCCT ACAACAGTTC AGACCGGGGA 
AGTAGTACAT CAGGGAGTCA AGGGCACAAG AAAGGGGCAA GGACACCCAA AGTGCCAAAG 
CAAGGTGGCA TGAACTGGGC AGACCTACTG CCCCCTCCCC CAGCACATCC TCCTCCTCAT 
AGCAACAGTG AAGAGTACAG CATCTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGC 
CCCGTGCCCC CAGCACGGAT GTATCTGCAA CAAGGTGAAC TAGAAGAGGA GGAAGATGAA 
CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC GCCTCTTCTC CGGCTGCCGT GTCTTACAGC 
CATCAATCCA CCGCCACTCT CACGCCCTCT CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCCG AGGAGGTGGG CCATGGGCAG CACCAGCCTG ACAGGAGACG GCAGCCGGTG 
AGCCCACCTC CACCTCCAAG GCCGATTTCC CCACCCCACA CCTACGGCTA CATTTCAGGA 
CCCCTTGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAAGTAGCCA AGATGCAGAC CAGGAGGCTT TTGTTACGTG GGCTGGAGCA GACCCCTGCC 
TCCAGTGTCG GGGACCTGGA GAGCTCTGTC ACCGGGTCCA TGATCAATGG TTGGGGCTCT 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGCG TTAGTTCTTC TGATGGCTCT 
TTTTTCACTG ATGCCGATTT TGCCCAGGCG GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG 
AAAGTGGCCC GGCGCCAAAT GCAGGATGCT GCTGGCCGCC GCCATTTTCA TGCATCCCAG 
TGCCCTAGAC CCACAAGTCC TGTTTCTACA GACAGCAACG TGAGTGCTGC TGTAGTACAG 
AAGGCCAGAC CAGCCAAGAA ACAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC 
ACAGATGATC TTCCACCTCC CCCTGTGCCA CCACCTGCTA TCAAGTCACC CACTGCCCAG 
TCCAAGGCCC AGCTGGAGGT TCGACCCGTG ATGGTGCCAA AACTCCCTTC TATAGAAGCA 
AGAACAGACA GATCATCAGA TAGGAAAGGA GGCAATTACA AGGGGAGAGA AGTATTGGAT 
GGAAGACAAG TTGCTGATAT GCGACCAAAT CCAGGTGATG CCAGAGAAGC ACAGGAACAG 
CCAAATGACG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
GCTCATCTCA TCCAAGAGGA TATTCTGCCT TACTGTAGAC CTACTTTTCC GACATCAAAT 
AATCCCAGAG ATCCTAGTTC TTCAAGTTCT ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAGA TGCAAGTTCT TGGAGGATAT 
GAAAGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201168.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201225.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

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CloneID	OBa15495
Clone ID Related Accession (Same CDS sequence)	XM_005201168.3 , XM_005201168.2
Accession Version	XM_005201168.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005201168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG 
AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT 
GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG 
GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACCCCTTA TGCCACCACC 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGTGGAGA CTCTGGCGAG 
AAGCACTGGA AGCCACCCGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGTGCAAATG ACACAATTGC TCCAACTATC 
CCTTACAACC AGTCATATGA CCAGAATACT GGAGGATCCT ACAACAGTTC AGACCGGGGA 
AGTAGTACAT CAGGGAGTCA AGGGCACAAG AAAGGGGCAA GGACACCCAA AGTGCCAAAG 
CAAGGTGGCA TGAACTGGGC AGACCTACTG CCCCCTCCCC CAGCACATCC TCCTCCTCAT 
AGCAACAGTG AAGAGTACAG CATCTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGC 
CCCGTGCCCC CAGCACGGAT GTATCTGCAA CAAGGTGAAC TAGAAGAGGA GGAAGATGAA 
CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC GCCTCTTCTC CGGCTGCCGT GTCTTACAGC 
CATCAATCCA CCGCCACTCT CACGCCCTCT CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCCG AGGAGGTGGG CCATGGGCAG CACCAGCCTG ACAGGAGACG GCAGCCGGTG 
AGCCCACCTC CACCTCCAAG GCCGATTTCC CCACCCCACA CCTACGGCTA CATTTCAGGA 
CCCCTTGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAAGTAGCCA AGATGCAGAC CAGGAGGCTT TTGTTACGTG GGCTGGAGCA GACCCCTGCC 
TCCAGTGTCG GGGACCTGGA GAGCTCTGTC ACCGGGTCCA TGATCAATGG TTGGGGCTCT 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGCG TTAGTTCTTC TGATGGCTCT 
TTTTTCACTG ATGCCGATTT TGCCCAGGCG GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG 
AAAGTGGCCC GGCGCCAAAT GCAGGATGCT GCTGGCCGCC GCCATTTTCA TGCATCCCAG 
TGCCCTAGAC CCACAAGTCC TGTTTCTACA GACAGCAACG TGAGTGCTGC TGTAGTACAG 
AAGGCCAGAC CAGCCAAGAA ACAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC 
ACAGATGATC TTCCACCTCC CCCTGTGCCA CCACCTGCTA TCAAGTCACC CACTGCCCAG 
TCCAAGGCCC AGCTGGAGGT TCGACCCGTG ATGGTGCCAA AACTCCCTTC TATAGAAGCA 
AGAACAGACA GATCATCAGA TAGGAAAGGA GGCAATTACA AGGGGAGAGA AGTATTGGAT 
GGAAGACAAG TTGCTGATAT GCGACCAAAT CCAGGTGATG CCAGAGAAGC ACAGGAACAG 
CCAAATGACG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
GCTCATCTCA TCCAAGAGGA TATTCTGCCT TACTGTAGAC CTACTTTTCC GACATCAAAT 
AATCCCAGAG ATCCTAGTTC TTCAAGTTCT ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAGA TGCAAGTTCT TGGAGGATAT 
GAAAGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201225.1
CDS	1014..5858
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201168.2
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201168.2

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG 
AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT 
GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG 
GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACCCCTTA TGCCACCACC 
CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGTGGAGA CTCTGGCGAG 
AAGCACTGGA AGCCACCCGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGTGCAAATG ACACAATTGC TCCAACTATC 
CCTTACAACC AGTCATATGA CCAGAATACT GGAGGATCCT ACAACAGTTC AGACCGGGGA 
AGTAGTACAT CAGGGAGTCA AGGGCACAAG AAAGGGGCAA GGACACCCAA AGTGCCAAAG 
CAAGGTGGCA TGAACTGGGC AGACCTACTG CCCCCTCCCC CAGCACATCC TCCTCCTCAT 
AGCAACAGTG AAGAGTACAG CATCTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGC 
CCCGTGCCCC CAGCACGGAT GTATCTGCAA CAAGGTGAAC TAGAAGAGGA GGAAGATGAA 
CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC GCCTCTTCTC CGGCTGCCGT GTCTTACAGC 
CATCAATCCA CCGCCACTCT CACGCCCTCT CCACAGGAAG AACTCCAGCC CATGTTACAG 
GATTGTCCCG AGGAGGTGGG CCATGGGCAG CACCAGCCTG ACAGGAGACG GCAGCCGGTG 
AGCCCACCTC CACCTCCAAG GCCGATTTCC CCACCCCACA CCTACGGCTA CATTTCAGGA 
CCCCTTGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG 
GAAGTAGCCA AGATGCAGAC CAGGAGGCTT TTGTTACGTG GGCTGGAGCA GACCCCTGCC 
TCCAGTGTCG GGGACCTGGA GAGCTCTGTC ACCGGGTCCA TGATCAATGG TTGGGGCTCT 
GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGCG TTAGTTCTTC TGATGGCTCT 
TTTTTCACTG ATGCCGATTT TGCCCAGGCG GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG 
AAAGTGGCCC GGCGCCAAAT GCAGGATGCT GCTGGCCGCC GCCATTTTCA TGCATCCCAG 
TGCCCTAGAC CCACAAGTCC TGTTTCTACA GACAGCAACG TGAGTGCTGC TGTAGTACAG 
AAGGCCAGAC CAGCCAAGAA ACAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC 
ACAGATGATC TTCCACCTCC CCCTGTGCCA CCACCTGCTA TCAAGTCACC CACTGCCCAG 
TCCAAGGCCC AGCTGGAGGT TCGACCCGTG ATGGTGCCAA AACTCCCTTC TATAGAAGCA 
AGAACAGACA GATCATCAGA TAGGAAAGGA GGCAATTACA AGGGGAGAGA AGTATTGGAT 
GGAAGACAAG TTGCTGATAT GCGACCAAAT CCAGGTGATG CCAGAGAAGC ACAGGAACAG 
CCAAATGACG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG 
GCTCATCTCA TCCAAGAGGA TATTCTGCCT TACTGTAGAC CTACTTTTCC GACATCAAAT 
AATCCCAGAG ATCCTAGTTC TTCAAGTTCT ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAGA TGCAAGTTCT TGGAGGATAT 
GAAAGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201169.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201226.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

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CloneID	OBa15496
Clone ID Related Accession (Same CDS sequence)	XM_005201169.3
Accession Version	XM_005201169.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTGGTTG CCTTGGGACG AACGGTCACT TTCCAGTGTG AAGCAACAGG AAATCCCCAA 
CCAGCTATCT TCTGGAGGAG AGAGGGGAGT CAGAATCTGC TTTTCTCATA TCAGCCACCA 
CAGTCATCCA GCCGTTTTTC AGTCTCCCAG ACTGGTGACC TCACTATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA TATCTGCCAG ACTTTGAATG TTGCCGGAAG TATCATCACA 
AAAGCATATT TGGAAGTTAC CGATGTGATT GCAGACCGAC CTCCCCCAGT CATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGTACTTTAG TGCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACAAT TCTGTGGAGA AAGGATGGAG TCCTTGTTTC AACCCAAGAT 
TCTCGAATCA AACAGCTGGA GACCGGAGTA CTACAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGAT ACACGTGCAT TGCATCAACC CCCAGTGGTG AAGCAACGTG GAGTGCTTAC 
ATTGAAGTCC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCCACCGA CCCAAATTTA 
ATCCCTAGTG CCCCCTCAAA ACCTGAAGTT ACAGATGTCA GCCGAAATAC AGTAACGTTG 
TCATGGCAAC CAAATTTGAA TTCAGGTGCA ACTCCGACGT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACT GTAGCGGAGA ATGTGAAAAC AGAAACATTT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCCAATGCA 
TATGGAATTA GTGATCCAAG CCAGATATCA GACCCAGTGA AAACACAAGA TGTTCCCCCA 
ACCAGTCAGG GGGTGGATCA CAAGCAGGTC CAGCGAGAAC TGGGAAATGT TGTACTGCAT 
CTCCACAACC CCACCATCCT TTCCTCCTCT TCAATCGAAG TGCACTGGAC AGTGGACCAA 
CAGTCTCAGT ATATTCAAGG ATACAAAGTT CTTTACCGCC CGTCTGGAGC CAACCATGGT 
GAATCCGAGT GGTTAGTTTT TGAAGTGAGG ACCCCAACCA AAAACAGTGT GGTTATCCCT 
GATCTCAAGA AAGGAGTCAA CTATGAAATT AAGGCTCGTC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATTAA ATTTGCCAAA ACCCTAGAAG AAGCACCCAG TGCCCCACCT 
CAAAGTGTGA CTGTATCAAA GAATGATGGA AATGGAACTG CAATTCTTGT TAGTTGGCAG 
CCACCTCCTG AAGATACTCA AAATGGAATG GTCCAAGAGT ATAAGGTCTG GTGTCTGGGC 
AATGAAACGC GATACCATAT AAACAAGACA GTAGATGGTT CCACCTTTTC TGTGGTTATT 
CCCTCACTGG TTCCTGGGAT CCGATATAGT GTAGAGGTGG CAGCCAGCAC GGGGGCTGGG 
TCGGGGGTGA AGAGTGAGCC ACAATTCATC CAGTTAGATT CTCATGGAAA CCCCGTGTCA 
CCTGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCAGACG TAGTGAAGCA GCCGGCATTC 
ATCGCAGGAA TCGGGGCTGC CTGTTGGATC ATCCTTATGG TTTTTAGCAT CTGGCTCTAT 
CGACACCGAA AGAAGAGAAA TGGGCTGGCT AGTACTTATG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAT CAGAGAGGAG GCGAAGCTGT GAGCAGTGGA 
GGGAGGCCAG GACTTCTCAA CATCAGTGAA CCTGCCACAC AGCCATGGCT TGCAGACACA 
TGGCCTAACA CTGGCAACAA TCACAATGAC TGCTCCATCA ATTGCTGCAC GGCAGGCAAT 
GGGAACAGTG ACAGCAACTT GACCACGTAT AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACCCCTT ATGCCACCAC CCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGTGGAG ACTCTGGCGA GAAGCACTGG 
AAGCCACCCG GACAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAACTGA ACAAAGATTA TCGTGCAAAT GACACAATTG CTCCAACTAT CCCTTACAAC 
CAGTCATATG ACCAGAATAC TGGAGGATCC TACAACAGTT CAGACCGGGG AAGTAGTACA 
TCAGGGAGTC AAGGGCACAA GAAAGGGGCA AGGACACCCA AAGTGCCAAA GCAAGGTGGC 
ATGAACTGGG CAGACCTACT GCCCCCTCCC CCAGCACATC CTCCTCCTCA TAGCAACAGT 
GAAGAGTACA GCATCTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG CCCCGTGCCC 
CCAGCACGGA TGTATCTGCA ACAAGGTGAA CTAGAAGAGG AGGAAGATGA ACGAGGTCCC 
ACTCCCCCTG TGCGGGGAGC CGCCTCTTCT CCGGCTGCCG TGTCTTACAG CCATCAATCC 
ACCGCCACTC TCACGCCCTC TCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCC 
GAGGAGGTGG GCCATGGGCA GCACCAGCCT GACAGGAGAC GGCAGCCGGT GAGCCCACCT 
CCACCTCCAA GGCCGATTTC CCCACCCCAC ACCTACGGCT ACATTTCAGG ACCCCTTGTC 
TCAGATATGG ATACGGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAAGTAGCC 
AAGATGCAGA CCAGGAGGCT TTTGTTACGT GGGCTGGAGC AGACCCCTGC CTCCAGTGTC 
GGGGACCTGG AGAGCTCTGT CACCGGGTCC ATGATCAATG GTTGGGGCTC TGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGC GTTAGTTCTT CTGATGGCTC TTTTTTCACT 
GATGCCGATT TTGCCCAGGC GGTCGCAGCA GCAGCGGAGT ATGCTGGTCT GAAAGTGGCC 
CGGCGCCAAA TGCAGGATGC TGCTGGCCGC CGCCATTTTC ATGCATCCCA GTGCCCTAGA 
CCCACAAGTC CTGTTTCTAC AGACAGCAAC GTGAGTGCTG CTGTAGTACA GAAGGCCAGA 
CCAGCCAAGA AACAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGCCTA CACAGATGAT 
CTTCCACCTC CCCCTGTGCC ACCACCTGCT ATCAAGTCAC CCACTGCCCA GTCCAAGGCC 
CAGCTGGAGG TTCGACCCGT GATGGTGCCA AAACTCCCTT CTATAGAAGC AAGAACAGAC 
AGATCATCAG ATAGGAAAGG AGGCAATTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAA 
GTTGCTGATA TGCGACCAAA TCCAGGTGAT GCCAGAGAAG CACAGGAACA GCCAAATGAC 
GGGAAAGGAC GAGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GGCTCATCTC 
ATCCAAGAGG ATATTCTGCC TTACTGTAGA CCTACTTTTC CGACATCAAA TAATCCCAGA 
GATCCTAGTT CTTCAAGTTC TATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAG ATGCAAGTTC TTGGAGGATA TGAAAGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201226.1
CDS	1..4836
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201169.3
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201169.3

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTGGTTG CCTTGGGACG AACGGTCACT TTCCAGTGTG AAGCAACAGG AAATCCCCAA 
CCAGCTATCT TCTGGAGGAG AGAGGGGAGT CAGAATCTGC TTTTCTCATA TCAGCCACCA 
CAGTCATCCA GCCGTTTTTC AGTCTCCCAG ACTGGTGACC TCACTATTAC TAATGTCCAG 
CGATCTGATG TTGGTTATTA TATCTGCCAG ACTTTGAATG TTGCCGGAAG TATCATCACA 
AAAGCATATT TGGAAGTTAC CGATGTGATT GCAGACCGAC CTCCCCCAGT CATTCGACAA 
GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGTACTTTAG TGCTCAGCTG TGTGGCCACA 
GGCAGTCCAG TGCCCACAAT TCTGTGGAGA AAGGATGGAG TCCTTGTTTC AACCCAAGAT 
TCTCGAATCA AACAGCTGGA GACCGGAGTA CTACAGATCC GATATGCTAA GCTGGGTGAT 
ACTGGTCGAT ACACGTGCAT TGCATCAACC CCCAGTGGTG AAGCAACGTG GAGTGCTTAC 
ATTGAAGTCC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCCACCGA CCCAAATTTA 
ATCCCTAGTG CCCCCTCAAA ACCTGAAGTT ACAGATGTCA GCCGAAATAC AGTAACGTTG 
TCATGGCAAC CAAATTTGAA TTCAGGTGCA ACTCCGACGT CTTATATTAT AGAAGCCTTC 
AGCCATGCAT CTGGTAGCAG CTGGCAGACT GTAGCGGAGA ATGTGAAAAC AGAAACATTT 
GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCCAATGCA 
TATGGAATTA GTGATCCAAG CCAGATATCA GACCCAGTGA AAACACAAGA TGTTCCCCCA 
ACCAGTCAGG GGGTGGATCA CAAGCAGGTC CAGCGAGAAC TGGGAAATGT TGTACTGCAT 
CTCCACAACC CCACCATCCT TTCCTCCTCT TCAATCGAAG TGCACTGGAC AGTGGACCAA 
CAGTCTCAGT ATATTCAAGG ATACAAAGTT CTTTACCGCC CGTCTGGAGC CAACCATGGT 
GAATCCGAGT GGTTAGTTTT TGAAGTGAGG ACCCCAACCA AAAACAGTGT GGTTATCCCT 
GATCTCAAGA AAGGAGTCAA CTATGAAATT AAGGCTCGTC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATTAA ATTTGCCAAA ACCCTAGAAG AAGCACCCAG TGCCCCACCT 
CAAAGTGTGA CTGTATCAAA GAATGATGGA AATGGAACTG CAATTCTTGT TAGTTGGCAG 
CCACCTCCTG AAGATACTCA AAATGGAATG GTCCAAGAGT ATAAGGTCTG GTGTCTGGGC 
AATGAAACGC GATACCATAT AAACAAGACA GTAGATGGTT CCACCTTTTC TGTGGTTATT 
CCCTCACTGG TTCCTGGGAT CCGATATAGT GTAGAGGTGG CAGCCAGCAC GGGGGCTGGG 
TCGGGGGTGA AGAGTGAGCC ACAATTCATC CAGTTAGATT CTCATGGAAA CCCCGTGTCA 
CCTGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCAGACG TAGTGAAGCA GCCGGCATTC 
ATCGCAGGAA TCGGGGCTGC CTGTTGGATC ATCCTTATGG TTTTTAGCAT CTGGCTCTAT 
CGACACCGAA AGAAGAGAAA TGGGCTGGCT AGTACTTATG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAT CAGAGAGGAG GCGAAGCTGT GAGCAGTGGA 
GGGAGGCCAG GACTTCTCAA CATCAGTGAA CCTGCCACAC AGCCATGGCT TGCAGACACA 
TGGCCTAACA CTGGCAACAA TCACAATGAC TGCTCCATCA ATTGCTGCAC GGCAGGCAAT 
GGGAACAGTG ACAGCAACTT GACCACGTAT AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACCCCTT ATGCCACCAC CCAGCTCATC 
CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGTGGAG ACTCTGGCGA GAAGCACTGG 
AAGCCACCCG GACAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAACTGA ACAAAGATTA TCGTGCAAAT GACACAATTG CTCCAACTAT CCCTTACAAC 
CAGTCATATG ACCAGAATAC TGGAGGATCC TACAACAGTT CAGACCGGGG AAGTAGTACA 
TCAGGGAGTC AAGGGCACAA GAAAGGGGCA AGGACACCCA AAGTGCCAAA GCAAGGTGGC 
ATGAACTGGG CAGACCTACT GCCCCCTCCC CCAGCACATC CTCCTCCTCA TAGCAACAGT 
GAAGAGTACA GCATCTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG CCCCGTGCCC 
CCAGCACGGA TGTATCTGCA ACAAGGTGAA CTAGAAGAGG AGGAAGATGA ACGAGGTCCC 
ACTCCCCCTG TGCGGGGAGC CGCCTCTTCT CCGGCTGCCG TGTCTTACAG CCATCAATCC 
ACCGCCACTC TCACGCCCTC TCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCC 
GAGGAGGTGG GCCATGGGCA GCACCAGCCT GACAGGAGAC GGCAGCCGGT GAGCCCACCT 
CCACCTCCAA GGCCGATTTC CCCACCCCAC ACCTACGGCT ACATTTCAGG ACCCCTTGTC 
TCAGATATGG ATACGGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAAGTAGCC 
AAGATGCAGA CCAGGAGGCT TTTGTTACGT GGGCTGGAGC AGACCCCTGC CTCCAGTGTC 
GGGGACCTGG AGAGCTCTGT CACCGGGTCC ATGATCAATG GTTGGGGCTC TGCCTCAGAG 
GAGGACAACA TTTCCAGCGG ACGCTCCAGC GTTAGTTCTT CTGATGGCTC TTTTTTCACT 
GATGCCGATT TTGCCCAGGC GGTCGCAGCA GCAGCGGAGT ATGCTGGTCT GAAAGTGGCC 
CGGCGCCAAA TGCAGGATGC TGCTGGCCGC CGCCATTTTC ATGCATCCCA GTGCCCTAGA 
CCCACAAGTC CTGTTTCTAC AGACAGCAAC GTGAGTGCTG CTGTAGTACA GAAGGCCAGA 
CCAGCCAAGA AACAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGCCTA CACAGATGAT 
CTTCCACCTC CCCCTGTGCC ACCACCTGCT ATCAAGTCAC CCACTGCCCA GTCCAAGGCC 
CAGCTGGAGG TTCGACCCGT GATGGTGCCA AAACTCCCTT CTATAGAAGC AAGAACAGAC 
AGATCATCAG ATAGGAAAGG AGGCAATTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAA 
GTTGCTGATA TGCGACCAAA TCCAGGTGAT GCCAGAGAAG CACAGGAACA GCCAAATGAC 
GGGAAAGGAC GAGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GGCTCATCTC 
ATCCAAGAGG ATATTCTGCC TTACTGTAGA CCTACTTTTC CGACATCAAA TAATCCCAGA 
GATCCTAGTT CTTCAAGTTC TATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAG ATGCAAGTTC TTGGAGGATA TGAAAGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201170.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201227.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

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CloneID	OBa15497
Clone ID Related Accession (Same CDS sequence)	XM_005201170.4 , XM_005201170.3
Accession Version	XM_005201170.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201170.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC 
AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC 
AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC 
TTGACCACGT ATAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCCACCCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGTGG AGACTCTGGC GAGAAGCACT GGAAGCCACC CGGACAGCAG 
AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT 
TATCGTGCAA ATGACACAAT TGCTCCAACT ATCCCTTACA ACCAGTCATA TGACCAGAAT 
ACTGGAGGAT CCTACAACAG TTCAGACCGG GGAAGTAGTA CATCAGGGAG TCAAGGGCAC 
AAGAAAGGGG CAAGGACACC CAAAGTGCCA AAGCAAGGTG GCATGAACTG GGCAGACCTA 
CTGCCCCCTC CCCCAGCACA TCCTCCTCCT CATAGCAACA GTGAAGAGTA CAGCATCTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGCCCCGTGC CCCCAGCACG GATGTATCTG 
CAACAAGGTG AACTAGAAGA GGAGGAAGAT GAACGAGGTC CCACTCCCCC TGTGCGGGGA 
GCCGCCTCTT CTCCGGCTGC CGTGTCTTAC AGCCATCAAT CCACCGCCAC TCTCACGCCC 
TCTCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CCGAGGAGGT GGGCCATGGG 
CAGCACCAGC CTGACAGGAG ACGGCAGCCG GTGAGCCCAC CTCCACCTCC AAGGCCGATT 
TCCCCACCCC ACACCTACGG CTACATTTCA GGACCCCTTG TCTCAGATAT GGATACGGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAAGTAG CCAAGATGCA GACCAGGAGG 
CTTTTGTTAC GTGGGCTGGA GCAGACCCCT GCCTCCAGTG TCGGGGACCT GGAGAGCTCT 
GTCACCGGGT CCATGATCAA TGGTTGGGGC TCTGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GCGTTAGTTC TTCTGATGGC TCTTTTTTCA CTGATGCCGA TTTTGCCCAG 
GCGGTCGCAG CAGCAGCGGA GTATGCTGGT CTGAAAGTGG CCCGGCGCCA AATGCAGGAT 
GCTGCTGGCC GCCGCCATTT TCATGCATCC CAGTGCCCTA GACCCACAAG TCCTGTTTCT 
ACAGACAGCA ACGTGAGTGC TGCTGTAGTA CAGAAGGCCA GACCAGCCAA GAAACAAAAA 
CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCCCCTGTG 
CCACCACCTG CTATCAAGTC ACCCACTGCC CAGTCCAAGG CCCAGCTGGA GGTTCGACCC 
GTGATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACAGATCATC AGATAGGAAA 
GGAGGCAATT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCTGA TATGCGACCA 
AATCCAGGTG ATGCCAGAGA AGCACAGGAA CAGCCAAATG ACGGGAAAGG ACGAGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGGCTCATC TCATCCAAGA GGATATTCTG 
CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGT 
TCTATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AGATGCAAGT TCTTGGAGGA TATGAAAGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201227.1
CDS	1113..5930
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201170.4
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201170.4

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC 
AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC 
AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC 
TTGACCACGT ATAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCCACCCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGTGG AGACTCTGGC GAGAAGCACT GGAAGCCACC CGGACAGCAG 
AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT 
TATCGTGCAA ATGACACAAT TGCTCCAACT ATCCCTTACA ACCAGTCATA TGACCAGAAT 
ACTGGAGGAT CCTACAACAG TTCAGACCGG GGAAGTAGTA CATCAGGGAG TCAAGGGCAC 
AAGAAAGGGG CAAGGACACC CAAAGTGCCA AAGCAAGGTG GCATGAACTG GGCAGACCTA 
CTGCCCCCTC CCCCAGCACA TCCTCCTCCT CATAGCAACA GTGAAGAGTA CAGCATCTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGCCCCGTGC CCCCAGCACG GATGTATCTG 
CAACAAGGTG AACTAGAAGA GGAGGAAGAT GAACGAGGTC CCACTCCCCC TGTGCGGGGA 
GCCGCCTCTT CTCCGGCTGC CGTGTCTTAC AGCCATCAAT CCACCGCCAC TCTCACGCCC 
TCTCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CCGAGGAGGT GGGCCATGGG 
CAGCACCAGC CTGACAGGAG ACGGCAGCCG GTGAGCCCAC CTCCACCTCC AAGGCCGATT 
TCCCCACCCC ACACCTACGG CTACATTTCA GGACCCCTTG TCTCAGATAT GGATACGGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAAGTAG CCAAGATGCA GACCAGGAGG 
CTTTTGTTAC GTGGGCTGGA GCAGACCCCT GCCTCCAGTG TCGGGGACCT GGAGAGCTCT 
GTCACCGGGT CCATGATCAA TGGTTGGGGC TCTGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GCGTTAGTTC TTCTGATGGC TCTTTTTTCA CTGATGCCGA TTTTGCCCAG 
GCGGTCGCAG CAGCAGCGGA GTATGCTGGT CTGAAAGTGG CCCGGCGCCA AATGCAGGAT 
GCTGCTGGCC GCCGCCATTT TCATGCATCC CAGTGCCCTA GACCCACAAG TCCTGTTTCT 
ACAGACAGCA ACGTGAGTGC TGCTGTAGTA CAGAAGGCCA GACCAGCCAA GAAACAAAAA 
CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCCCCTGTG 
CCACCACCTG CTATCAAGTC ACCCACTGCC CAGTCCAAGG CCCAGCTGGA GGTTCGACCC 
GTGATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACAGATCATC AGATAGGAAA 
GGAGGCAATT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCTGA TATGCGACCA 
AATCCAGGTG ATGCCAGAGA AGCACAGGAA CAGCCAAATG ACGGGAAAGG ACGAGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGGCTCATC TCATCCAAGA GGATATTCTG 
CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGT 
TCTATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AGATGCAAGT TCTTGGAGGA TATGAAAGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201170.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201227.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa15497
Clone ID Related Accession (Same CDS sequence)	XM_005201170.4 , XM_005201170.3
Accession Version	XM_005201170.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC 
AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC 
AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC 
TTGACCACGT ATAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCCACCCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGTGG AGACTCTGGC GAGAAGCACT GGAAGCCACC CGGACAGCAG 
AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT 
TATCGTGCAA ATGACACAAT TGCTCCAACT ATCCCTTACA ACCAGTCATA TGACCAGAAT 
ACTGGAGGAT CCTACAACAG TTCAGACCGG GGAAGTAGTA CATCAGGGAG TCAAGGGCAC 
AAGAAAGGGG CAAGGACACC CAAAGTGCCA AAGCAAGGTG GCATGAACTG GGCAGACCTA 
CTGCCCCCTC CCCCAGCACA TCCTCCTCCT CATAGCAACA GTGAAGAGTA CAGCATCTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGCCCCGTGC CCCCAGCACG GATGTATCTG 
CAACAAGGTG AACTAGAAGA GGAGGAAGAT GAACGAGGTC CCACTCCCCC TGTGCGGGGA 
GCCGCCTCTT CTCCGGCTGC CGTGTCTTAC AGCCATCAAT CCACCGCCAC TCTCACGCCC 
TCTCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CCGAGGAGGT GGGCCATGGG 
CAGCACCAGC CTGACAGGAG ACGGCAGCCG GTGAGCCCAC CTCCACCTCC AAGGCCGATT 
TCCCCACCCC ACACCTACGG CTACATTTCA GGACCCCTTG TCTCAGATAT GGATACGGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAAGTAG CCAAGATGCA GACCAGGAGG 
CTTTTGTTAC GTGGGCTGGA GCAGACCCCT GCCTCCAGTG TCGGGGACCT GGAGAGCTCT 
GTCACCGGGT CCATGATCAA TGGTTGGGGC TCTGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GCGTTAGTTC TTCTGATGGC TCTTTTTTCA CTGATGCCGA TTTTGCCCAG 
GCGGTCGCAG CAGCAGCGGA GTATGCTGGT CTGAAAGTGG CCCGGCGCCA AATGCAGGAT 
GCTGCTGGCC GCCGCCATTT TCATGCATCC CAGTGCCCTA GACCCACAAG TCCTGTTTCT 
ACAGACAGCA ACGTGAGTGC TGCTGTAGTA CAGAAGGCCA GACCAGCCAA GAAACAAAAA 
CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCCCCTGTG 
CCACCACCTG CTATCAAGTC ACCCACTGCC CAGTCCAAGG CCCAGCTGGA GGTTCGACCC 
GTGATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACAGATCATC AGATAGGAAA 
GGAGGCAATT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCTGA TATGCGACCA 
AATCCAGGTG ATGCCAGAGA AGCACAGGAA CAGCCAAATG ACGGGAAAGG ACGAGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGGCTCATC TCATCCAAGA GGATATTCTG 
CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGT 
TCTATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AGATGCAAGT TCTTGGAGGA TATGAAAGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201227.1
CDS	1..4818
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201170.3
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201170.3

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC 
AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC 
AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC 
TTGACCACGT ATAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCCACCCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC 
AACATGAACA ATGGCAGTGG AGACTCTGGC GAGAAGCACT GGAAGCCACC CGGACAGCAG 
AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT 
TATCGTGCAA ATGACACAAT TGCTCCAACT ATCCCTTACA ACCAGTCATA TGACCAGAAT 
ACTGGAGGAT CCTACAACAG TTCAGACCGG GGAAGTAGTA CATCAGGGAG TCAAGGGCAC 
AAGAAAGGGG CAAGGACACC CAAAGTGCCA AAGCAAGGTG GCATGAACTG GGCAGACCTA 
CTGCCCCCTC CCCCAGCACA TCCTCCTCCT CATAGCAACA GTGAAGAGTA CAGCATCTCT 
GTAGATGAAA GCTATGACCA AGAAATGCCA TGCCCCGTGC CCCCAGCACG GATGTATCTG 
CAACAAGGTG AACTAGAAGA GGAGGAAGAT GAACGAGGTC CCACTCCCCC TGTGCGGGGA 
GCCGCCTCTT CTCCGGCTGC CGTGTCTTAC AGCCATCAAT CCACCGCCAC TCTCACGCCC 
TCTCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CCGAGGAGGT GGGCCATGGG 
CAGCACCAGC CTGACAGGAG ACGGCAGCCG GTGAGCCCAC CTCCACCTCC AAGGCCGATT 
TCCCCACCCC ACACCTACGG CTACATTTCA GGACCCCTTG TCTCAGATAT GGATACGGAT 
GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAAGTAG CCAAGATGCA GACCAGGAGG 
CTTTTGTTAC GTGGGCTGGA GCAGACCCCT GCCTCCAGTG TCGGGGACCT GGAGAGCTCT 
GTCACCGGGT CCATGATCAA TGGTTGGGGC TCTGCCTCAG AGGAGGACAA CATTTCCAGC 
GGACGCTCCA GCGTTAGTTC TTCTGATGGC TCTTTTTTCA CTGATGCCGA TTTTGCCCAG 
GCGGTCGCAG CAGCAGCGGA GTATGCTGGT CTGAAAGTGG CCCGGCGCCA AATGCAGGAT 
GCTGCTGGCC GCCGCCATTT TCATGCATCC CAGTGCCCTA GACCCACAAG TCCTGTTTCT 
ACAGACAGCA ACGTGAGTGC TGCTGTAGTA CAGAAGGCCA GACCAGCCAA GAAACAAAAA 
CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCCCCTGTG 
CCACCACCTG CTATCAAGTC ACCCACTGCC CAGTCCAAGG CCCAGCTGGA GGTTCGACCC 
GTGATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACAGATCATC AGATAGGAAA 
GGAGGCAATT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCTGA TATGCGACCA 
AATCCAGGTG ATGCCAGAGA AGCACAGGAA CAGCCAAATG ACGGGAAAGG ACGAGGAAAC 
AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGGCTCATC TCATCCAAGA GGATATTCTG 
CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGT 
TCTATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AGATGCAAGT TCTTGGAGGA TATGAAAGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201171.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201228.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa15498
Clone ID Related Accession (Same CDS sequence)	XM_005201171.4 , XM_005201171.3
Accession Version	XM_005201171.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201171.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGGGCA GCCCACCCCT 
TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGTGGA 
GACTCTGGCG AGAAGCACTG GAAGCCACCC GGACAGCAGA AACAGGAAGT GGCACCAATT 
CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGTGCAAA TGACACAATT 
GCTCCAACTA TCCCTTACAA CCAGTCATAT GACCAGAATA CTGGAGGATC CTACAACAGT 
TCAGACCGGG GAAGTAGTAC ATCAGGGAGT CAAGGGCACA AGAAAGGGGC AAGGACACCC 
AAAGTGCCAA AGCAAGGTGG CATGAACTGG GCAGACCTAC TGCCCCCTCC CCCAGCACAT 
CCTCCTCCTC ATAGCAACAG TGAAGAGTAC AGCATCTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GCCCCGTGCC CCCAGCACGG ATGTATCTGC AACAAGGTGA ACTAGAAGAG 
GAGGAAGATG AACGAGGTCC CACTCCCCCT GTGCGGGGAG CCGCCTCTTC TCCGGCTGCC 
GTGTCTTACA GCCATCAATC CACCGCCACT CTCACGCCCT CTCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC CGAGGAGGTG GGCCATGGGC AGCACCAGCC TGACAGGAGA 
CGGCAGCCGG TGAGCCCACC TCCACCTCCA AGGCCGATTT CCCCACCCCA CACCTACGGC 
TACATTTCAG GACCCCTTGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAAGTAGC CAAGATGCAG ACCAGGAGGC TTTTGTTACG TGGGCTGGAG 
CAGACCCCTG CCTCCAGTGT CGGGGACCTG GAGAGCTCTG TCACCGGGTC CATGATCAAT 
GGTTGGGGCT CTGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG CGTTAGTTCT 
TCTGATGGCT CTTTTTTCAC TGATGCCGAT TTTGCCCAGG CGGTCGCAGC AGCAGCGGAG 
TATGCTGGTC TGAAAGTGGC CCGGCGCCAA ATGCAGGATG CTGCTGGCCG CCGCCATTTT 
CATGCATCCC AGTGCCCTAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CGTGAGTGCT 
GCTGTAGTAC AGAAGGCCAG ACCAGCCAAG AAACAAAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAGCCT ACACAGATGA TCTTCCACCT CCCCCTGTGC CACCACCTGC TATCAAGTCA 
CCCACTGCCC AGTCCAAGGC CCAGCTGGAG GTTCGACCCG TGATGGTGCC AAAACTCCCT 
TCTATAGAAG CAAGAACAGA CAGATCATCA GATAGGAAAG GAGGCAATTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA AGTTGCTGAT ATGCGACCAA ATCCAGGTGA TGCCAGAGAA 
GCACAGGAAC AGCCAAATGA CGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGGCTCATCT CATCCAAGAG GATATTCTGC CTTACTGTAG ACCTACTTTT 
CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGTT CTATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA GATGCAAGTT 
CTTGGAGGAT ATGAAAGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201228.1
CDS	297..5093
Translation	MKWTHAPFLAIISLLSLSKSHLFLAQLIPDPEDVERGKDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201171.4
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201171.4

ORF Insert Sequence:

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGGGCA GCCCACCCCT 
TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGTGGA 
GACTCTGGCG AGAAGCACTG GAAGCCACCC GGACAGCAGA AACAGGAAGT GGCACCAATT 
CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGTGCAAA TGACACAATT 
GCTCCAACTA TCCCTTACAA CCAGTCATAT GACCAGAATA CTGGAGGATC CTACAACAGT 
TCAGACCGGG GAAGTAGTAC ATCAGGGAGT CAAGGGCACA AGAAAGGGGC AAGGACACCC 
AAAGTGCCAA AGCAAGGTGG CATGAACTGG GCAGACCTAC TGCCCCCTCC CCCAGCACAT 
CCTCCTCCTC ATAGCAACAG TGAAGAGTAC AGCATCTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GCCCCGTGCC CCCAGCACGG ATGTATCTGC AACAAGGTGA ACTAGAAGAG 
GAGGAAGATG AACGAGGTCC CACTCCCCCT GTGCGGGGAG CCGCCTCTTC TCCGGCTGCC 
GTGTCTTACA GCCATCAATC CACCGCCACT CTCACGCCCT CTCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC CGAGGAGGTG GGCCATGGGC AGCACCAGCC TGACAGGAGA 
CGGCAGCCGG TGAGCCCACC TCCACCTCCA AGGCCGATTT CCCCACCCCA CACCTACGGC 
TACATTTCAG GACCCCTTGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAAGTAGC CAAGATGCAG ACCAGGAGGC TTTTGTTACG TGGGCTGGAG 
CAGACCCCTG CCTCCAGTGT CGGGGACCTG GAGAGCTCTG TCACCGGGTC CATGATCAAT 
GGTTGGGGCT CTGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG CGTTAGTTCT 
TCTGATGGCT CTTTTTTCAC TGATGCCGAT TTTGCCCAGG CGGTCGCAGC AGCAGCGGAG 
TATGCTGGTC TGAAAGTGGC CCGGCGCCAA ATGCAGGATG CTGCTGGCCG CCGCCATTTT 
CATGCATCCC AGTGCCCTAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CGTGAGTGCT 
GCTGTAGTAC AGAAGGCCAG ACCAGCCAAG AAACAAAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAGCCT ACACAGATGA TCTTCCACCT CCCCCTGTGC CACCACCTGC TATCAAGTCA 
CCCACTGCCC AGTCCAAGGC CCAGCTGGAG GTTCGACCCG TGATGGTGCC AAAACTCCCT 
TCTATAGAAG CAAGAACAGA CAGATCATCA GATAGGAAAG GAGGCAATTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA AGTTGCTGAT ATGCGACCAA ATCCAGGTGA TGCCAGAGAA 
GCACAGGAAC AGCCAAATGA CGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGGCTCATCT CATCCAAGAG GATATTCTGC CTTACTGTAG ACCTACTTTT 
CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGTT CTATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA GATGCAAGTT 
CTTGGAGGAT ATGAAAGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201171.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201228.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

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CloneID	OBa15498
Clone ID Related Accession (Same CDS sequence)	XM_005201171.4 , XM_005201171.3
Accession Version	XM_005201171.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGGGCA GCCCACCCCT 
TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGTGGA 
GACTCTGGCG AGAAGCACTG GAAGCCACCC GGACAGCAGA AACAGGAAGT GGCACCAATT 
CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGTGCAAA TGACACAATT 
GCTCCAACTA TCCCTTACAA CCAGTCATAT GACCAGAATA CTGGAGGATC CTACAACAGT 
TCAGACCGGG GAAGTAGTAC ATCAGGGAGT CAAGGGCACA AGAAAGGGGC AAGGACACCC 
AAAGTGCCAA AGCAAGGTGG CATGAACTGG GCAGACCTAC TGCCCCCTCC CCCAGCACAT 
CCTCCTCCTC ATAGCAACAG TGAAGAGTAC AGCATCTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GCCCCGTGCC CCCAGCACGG ATGTATCTGC AACAAGGTGA ACTAGAAGAG 
GAGGAAGATG AACGAGGTCC CACTCCCCCT GTGCGGGGAG CCGCCTCTTC TCCGGCTGCC 
GTGTCTTACA GCCATCAATC CACCGCCACT CTCACGCCCT CTCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC CGAGGAGGTG GGCCATGGGC AGCACCAGCC TGACAGGAGA 
CGGCAGCCGG TGAGCCCACC TCCACCTCCA AGGCCGATTT CCCCACCCCA CACCTACGGC 
TACATTTCAG GACCCCTTGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAAGTAGC CAAGATGCAG ACCAGGAGGC TTTTGTTACG TGGGCTGGAG 
CAGACCCCTG CCTCCAGTGT CGGGGACCTG GAGAGCTCTG TCACCGGGTC CATGATCAAT 
GGTTGGGGCT CTGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG CGTTAGTTCT 
TCTGATGGCT CTTTTTTCAC TGATGCCGAT TTTGCCCAGG CGGTCGCAGC AGCAGCGGAG 
TATGCTGGTC TGAAAGTGGC CCGGCGCCAA ATGCAGGATG CTGCTGGCCG CCGCCATTTT 
CATGCATCCC AGTGCCCTAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CGTGAGTGCT 
GCTGTAGTAC AGAAGGCCAG ACCAGCCAAG AAACAAAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAGCCT ACACAGATGA TCTTCCACCT CCCCCTGTGC CACCACCTGC TATCAAGTCA 
CCCACTGCCC AGTCCAAGGC CCAGCTGGAG GTTCGACCCG TGATGGTGCC AAAACTCCCT 
TCTATAGAAG CAAGAACAGA CAGATCATCA GATAGGAAAG GAGGCAATTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA AGTTGCTGAT ATGCGACCAA ATCCAGGTGA TGCCAGAGAA 
GCACAGGAAC AGCCAAATGA CGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGGCTCATCT CATCCAAGAG GATATTCTGC CTTACTGTAG ACCTACTTTT 
CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGTT CTATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA GATGCAAGTT 
CTTGGAGGAT ATGAAAGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201228.1
CDS	297..5093
Translation	MKWTHAPFLAIISLLSLSKSHLFLAQLIPDPEDVERGKDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201171.3
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201171.3

ORF Insert Sequence:

ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC 
GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA 
AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA 
TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT 
GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG 
GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA 
CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT 
GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG 
AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG 
GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA 
GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT 
CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG 
CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG 
GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA 
ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA 
GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA 
ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT 
CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA 
CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG 
GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC 
CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT 
ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC 
CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT 
CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG 
GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG 
GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC 
GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG 
GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG 
CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC 
AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA 
GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA 
GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGGGCA GCCCACCCCT 
TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGTGGA 
GACTCTGGCG AGAAGCACTG GAAGCCACCC GGACAGCAGA AACAGGAAGT GGCACCAATT 
CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGTGCAAA TGACACAATT 
GCTCCAACTA TCCCTTACAA CCAGTCATAT GACCAGAATA CTGGAGGATC CTACAACAGT 
TCAGACCGGG GAAGTAGTAC ATCAGGGAGT CAAGGGCACA AGAAAGGGGC AAGGACACCC 
AAAGTGCCAA AGCAAGGTGG CATGAACTGG GCAGACCTAC TGCCCCCTCC CCCAGCACAT 
CCTCCTCCTC ATAGCAACAG TGAAGAGTAC AGCATCTCTG TAGATGAAAG CTATGACCAA 
GAAATGCCAT GCCCCGTGCC CCCAGCACGG ATGTATCTGC AACAAGGTGA ACTAGAAGAG 
GAGGAAGATG AACGAGGTCC CACTCCCCCT GTGCGGGGAG CCGCCTCTTC TCCGGCTGCC 
GTGTCTTACA GCCATCAATC CACCGCCACT CTCACGCCCT CTCCACAGGA AGAACTCCAG 
CCCATGTTAC AGGATTGTCC CGAGGAGGTG GGCCATGGGC AGCACCAGCC TGACAGGAGA 
CGGCAGCCGG TGAGCCCACC TCCACCTCCA AGGCCGATTT CCCCACCCCA CACCTACGGC 
TACATTTCAG GACCCCTTGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC 
GAAGCCGACA TGGAAGTAGC CAAGATGCAG ACCAGGAGGC TTTTGTTACG TGGGCTGGAG 
CAGACCCCTG CCTCCAGTGT CGGGGACCTG GAGAGCTCTG TCACCGGGTC CATGATCAAT 
GGTTGGGGCT CTGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG CGTTAGTTCT 
TCTGATGGCT CTTTTTTCAC TGATGCCGAT TTTGCCCAGG CGGTCGCAGC AGCAGCGGAG 
TATGCTGGTC TGAAAGTGGC CCGGCGCCAA ATGCAGGATG CTGCTGGCCG CCGCCATTTT 
CATGCATCCC AGTGCCCTAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CGTGAGTGCT 
GCTGTAGTAC AGAAGGCCAG ACCAGCCAAG AAACAAAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAGCCT ACACAGATGA TCTTCCACCT CCCCCTGTGC CACCACCTGC TATCAAGTCA 
CCCACTGCCC AGTCCAAGGC CCAGCTGGAG GTTCGACCCG TGATGGTGCC AAAACTCCCT 
TCTATAGAAG CAAGAACAGA CAGATCATCA GATAGGAAAG GAGGCAATTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA AGTTGCTGAT ATGCGACCAA ATCCAGGTGA TGCCAGAGAA 
GCACAGGAAC AGCCAAATGA CGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGAGACCTT 
CCACCAGCAA AGGCTCATCT CATCCAAGAG GATATTCTGC CTTACTGTAG ACCTACTTTT 
CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGTT CTATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA GATGCAAGTT 
CTTGGAGGAT ATGAAAGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201172.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201229.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

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CloneID	OBa15499
Clone ID Related Accession (Same CDS sequence)	XM_005201172.3 , XM_005201172.4
Accession Version	XM_005201172.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC 
AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC 
AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC 
TTGACCACGT ATAGTCGCCC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG 
CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG 
TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA 
GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG 
AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA 
GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA 
GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT 
CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC 
GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG 
GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA 
CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG 
ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG 
GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT 
GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG 
CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA 
GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG 
CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA 
TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC 
CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201229.1
CDS	1..4653
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201172.3
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201172.3

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC 
AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC 
AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC 
TTGACCACGT ATAGTCGCCC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG 
CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG 
TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA 
GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG 
AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA 
GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA 
GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT 
CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC 
GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG 
GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA 
CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG 
ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG 
GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT 
GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG 
CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA 
GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG 
CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA 
TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC 
CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_005201172.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201229.1 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

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CloneID	OBa15499
Clone ID Related Accession (Same CDS sequence)	XM_005201172.3 , XM_005201172.4
Accession Version	XM_005201172.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	roundabout homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201172.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC 
AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC 
AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC 
TTGACCACGT ATAGTCGCCC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG 
CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG 
TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA 
GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG 
AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA 
GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA 
GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT 
CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC 
GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG 
GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA 
CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG 
ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG 
GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT 
GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG 
CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA 
GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG 
CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA 
TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC 
CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201229.1
CDS	1112..5764
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKVLYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLASTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIAPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQGELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEVGHGQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNVSAAVVQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTAQSKAQLEVRPVMVPKLPSIEARTDRSSDRKGGNYKGREVLDGRQVADMRPNPGDAREAQEQPNDGKGRGNKAAKRDLPPAKAHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYEREDNNEELEETES

Target ORF information:

RefSeq Version	XM_005201172.4
Organism	Bos taurus(cattle)
Definition	Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201172.4

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC 
TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC 
GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC 
AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC 
ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT 
ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT 
TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA 
GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT 
GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT 
GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA 
AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT 
CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT 
AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG 
CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA 
GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA 
GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA 
GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA 
GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT 
GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT 
GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA 
GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC 
AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG 
GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT 
GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG 
TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT 
GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG 
GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC 
CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG 
CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC 
TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC 
AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC 
AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC 
TTGACCACGT ATAGTCGCCC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG 
TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG 
CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG 
TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA 
GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG 
AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA 
GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA 
GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT 
CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC 
GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG 
GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA 
CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA 
GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG 
ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG 
GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG 
GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT 
GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG 
CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA 
GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG 
CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA 
TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC 
CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT 
AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ROBO1
Entrez Gene ID	536815
Full Name	roundabout guidance receptor 1
General protein information	Preferred Names roundabout guidance receptor 1 Names roundabout homolog 1 roundabout, axon guidance receptor, homolog 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010242	XP_554155.3
Danio rerio (zebrafish)	robo1	NP_571556.1
Drosophila melanogaster (fruit fly)	robo3	NP_001259866.1
Homo sapiens (human)	ROBO1	NP_002932.1
Pan troglodytes (chimpanzee)	ROBO1	XP_516592.3
Gallus gallus (chicken)	ROBO1	XP_004938403.1
Caenorhabditis elegans (roundworm)	sax-3	NP_001024990.1
Xenopus tropicalis (tropical clawed frog)	robo1	NP_001096171.1
	ROBO1	XP_002802665.1
Canis lupus familiaris (dog)	ROBO1	XP_544814.4
Bos taurus (cattle)	ROBO1	NP_001179817.2
Mus musculus (house mouse)	Robo1	NP_062286.2
Rattus norvegicus (Norway rat)	Robo1	NP_071524.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ROBO1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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