spna2 cDNA ORF clone, Danio rerio(zebrafish)

The following spna2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spna2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa150885 XM_021468823.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa150886 XM_021468824.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa150886 XM_021468826.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa150886 XM_021468825.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa150887 XM_021468827.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa150888 XM_021468828.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa150889 XM_021468829.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa150890 XM_021468832.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa33721 NM_001098488.1
Latest version!
Danio rerio spectrin alpha, non-erythrocytic 1 (sptan1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41932 XM_009295198.2
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41933 XM_009295199.2
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41933 XM_021468830.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41934 XM_021468831.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41934 XM_009295200.2
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41935 XM_009295201.2
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41936 XM_009295202.2
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41937 XM_021468833.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41937 XM_009295203.2
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41938 XM_009295204.2
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41939 XM_021468834.1
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa41939 XM_009295205.2
Latest version!
Danio rerio spectrin alpha 2 (spna2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa150885
    Clone ID Related Accession (Same CDS sequence) XM_021468823.1
    Accession Version XM_021468823.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GCATAGCCTA TCGGAGGGTA
    ATCCGTGTCT ATCAGTATGA AGTCGATGAT GATCTTTCTG GAAGGTCCTG CATGGTGGAA
    GAATCTGGAA CTCTAGAGTC ACAGCTAGAA GCCACTAAGC GTAAGCATCA GGAGATTCGT
    GCCATGCGCA GTCAGCTGAA GAAGATTGAG GATCTGGGAG CTGCCATGGA GGAGGCGCTG
    ATTCTGGACA ATAAATACAC AGAACACAGC ACCGTGGGTC TGGCACAACA GTGGGACCAA
    CTCGACCAGC TGGGCATGAG AATGCAACAT AACCTGGAAC AACAGATCCA AGCCAGAAAC
    ACTACAGGTG TAACAGAAGA GGCCCTGAAG GAGTTCAGCA TGATGTTCAA GCACTTCGAC
    AAAGAAAAAT CTGGCCGTCT GAATCACCAG GAGTTCAAGT CTTGTCTGCG GTCTCTGGGT
    TACGACCTTC CCATGGTGGA AGAAGGAGAA CCAGACCCAG AGTTTGAGTC CATCCTGGAT
    ATAGTGGATC CAAACAGGGA TGGAAACGTG TCCTTGCAGG AGTACATGGC TTTCATGATC
    AGCCGAGAAA CGGAGAACGT CAAGTCAAGC GAGGAGATCG AGAGCGCTTT CCGTGCTCTG
    AGCGCAGAAA ACAAACCCTA CGTCACCAAG GAAGAACTCT ACCAGAACCT GACAAAGGAA
    CAAGCCGACT ACTGCATTTC CCATATGAAG CCCTACCTAG ACAGCAAGGG CCGCGAAATC
    CCATCAGCTT TCGACTTTGT GGAATTCACC CGCTCGCTCT TCGTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324498.1
    CDS4..7494
    Translation

    Target ORF information:

    RefSeq Version XM_021468823.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468823.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GCATAGCCTA TCGGAGGGTA
    ATCCGTGTCT ATCAGTATGA AGTCGATGAT GATCTTTCTG GAAGGTCCTG CATGGTGGAA
    GAATCTGGAA CTCTAGAGTC ACAGCTAGAA GCCACTAAGC GTAAGCATCA GGAGATTCGT
    GCCATGCGCA GTCAGCTGAA GAAGATTGAG GATCTGGGAG CTGCCATGGA GGAGGCGCTG
    ATTCTGGACA ATAAATACAC AGAACACAGC ACCGTGGGTC TGGCACAACA GTGGGACCAA
    CTCGACCAGC TGGGCATGAG AATGCAACAT AACCTGGAAC AACAGATCCA AGCCAGAAAC
    ACTACAGGTG TAACAGAAGA GGCCCTGAAG GAGTTCAGCA TGATGTTCAA GCACTTCGAC
    AAAGAAAAAT CTGGCCGTCT GAATCACCAG GAGTTCAAGT CTTGTCTGCG GTCTCTGGGT
    TACGACCTTC CCATGGTGGA AGAAGGAGAA CCAGACCCAG AGTTTGAGTC CATCCTGGAT
    ATAGTGGATC CAAACAGGGA TGGAAACGTG TCCTTGCAGG AGTACATGGC TTTCATGATC
    AGCCGAGAAA CGGAGAACGT CAAGTCAAGC GAGGAGATCG AGAGCGCTTT CCGTGCTCTG
    AGCGCAGAAA ACAAACCCTA CGTCACCAAG GAAGAACTCT ACCAGAACCT GACAAAGGAA
    CAAGCCGACT ACTGCATTTC CCATATGAAG CCCTACCTAG ACAGCAAGGG CCGCGAAATC
    CCATCAGCTT TCGACTTTGT GGAATTCACC CGCTCGCTCT TCGTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150886
    Clone ID Related Accession (Same CDS sequence) XM_021468825.1 , XM_021468826.1 , XM_021468824.1
    Accession Version XM_021468825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
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    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGCATA
    GCCTATCGGA GGGTAATCCG TGTCTATCAG TATGAAGTCG ATGATGATCT TTCTGGAAGG
    TCCTGCATGG TGGAAGAATC TGGAACTCTA GAGTCACAGC TAGAAGCCAC TAAGCGTAAG
    CATCAGGAGA TTCGTGCCAT GCGCAGTCAG CTGAAGAAGA TTGAGGATCT GGGAGCTGCC
    ATGGAGGAGG CGCTGATTCT GGACAATAAA TACACAGAAC ACAGCACCGT GGGTCTGGCA
    CAACAGTGGG ACCAACTCGA CCAGCTGGGC ATGAGAATGC AACATAACCT GGAACAACAG
    ATCCAAGCCA GAAACACTAC AGGTGTAACA GAAGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TCGACAAAGA AAAATCTGGC CGTCTGAATC ACCAGGAGTT CAAGTCTTGT
    CTGCGGTCTC TGGGTTACGA CCTTCCCATG GTGGAAGAAG GAGAACCAGA CCCAGAGTTT
    GAGTCCATCC TGGATATAGT GGATCCAAAC AGGGATGGAA ACGTGTCCTT GCAGGAGTAC
    ATGGCTTTCA TGATCAGCCG AGAAACGGAG AACGTCAAGT CAAGCGAGGA GATCGAGAGC
    GCTTTCCGTG CTCTGAGCGC AGAAAACAAA CCCTACGTCA CCAAGGAAGA ACTCTACCAG
    AACCTGACAA AGGAACAAGC CGACTACTGC ATTTCCCATA TGAAGCCCTA CCTAGACAGC
    AAGGGCCGCG AAATCCCATC AGCTTTCGAC TTTGTGGAAT TCACCCGCTC GCTCTTCGTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324500.1
    CDS215..7720
    Translation

    Target ORF information:

    RefSeq Version XM_021468825.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGCATA
    GCCTATCGGA GGGTAATCCG TGTCTATCAG TATGAAGTCG ATGATGATCT TTCTGGAAGG
    TCCTGCATGG TGGAAGAATC TGGAACTCTA GAGTCACAGC TAGAAGCCAC TAAGCGTAAG
    CATCAGGAGA TTCGTGCCAT GCGCAGTCAG CTGAAGAAGA TTGAGGATCT GGGAGCTGCC
    ATGGAGGAGG CGCTGATTCT GGACAATAAA TACACAGAAC ACAGCACCGT GGGTCTGGCA
    CAACAGTGGG ACCAACTCGA CCAGCTGGGC ATGAGAATGC AACATAACCT GGAACAACAG
    ATCCAAGCCA GAAACACTAC AGGTGTAACA GAAGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TCGACAAAGA AAAATCTGGC CGTCTGAATC ACCAGGAGTT CAAGTCTTGT
    CTGCGGTCTC TGGGTTACGA CCTTCCCATG GTGGAAGAAG GAGAACCAGA CCCAGAGTTT
    GAGTCCATCC TGGATATAGT GGATCCAAAC AGGGATGGAA ACGTGTCCTT GCAGGAGTAC
    ATGGCTTTCA TGATCAGCCG AGAAACGGAG AACGTCAAGT CAAGCGAGGA GATCGAGAGC
    GCTTTCCGTG CTCTGAGCGC AGAAAACAAA CCCTACGTCA CCAAGGAAGA ACTCTACCAG
    AACCTGACAA AGGAACAAGC CGACTACTGC ATTTCCCATA TGAAGCCCTA CCTAGACAGC
    AAGGGCCGCG AAATCCCATC AGCTTTCGAC TTTGTGGAAT TCACCCGCTC GCTCTTCGTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150886
    Clone ID Related Accession (Same CDS sequence) XM_021468825.1 , XM_021468826.1 , XM_021468824.1
    Accession Version XM_021468826.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGCATA
    GCCTATCGGA GGGTAATCCG TGTCTATCAG TATGAAGTCG ATGATGATCT TTCTGGAAGG
    TCCTGCATGG TGGAAGAATC TGGAACTCTA GAGTCACAGC TAGAAGCCAC TAAGCGTAAG
    CATCAGGAGA TTCGTGCCAT GCGCAGTCAG CTGAAGAAGA TTGAGGATCT GGGAGCTGCC
    ATGGAGGAGG CGCTGATTCT GGACAATAAA TACACAGAAC ACAGCACCGT GGGTCTGGCA
    CAACAGTGGG ACCAACTCGA CCAGCTGGGC ATGAGAATGC AACATAACCT GGAACAACAG
    ATCCAAGCCA GAAACACTAC AGGTGTAACA GAAGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TCGACAAAGA AAAATCTGGC CGTCTGAATC ACCAGGAGTT CAAGTCTTGT
    CTGCGGTCTC TGGGTTACGA CCTTCCCATG GTGGAAGAAG GAGAACCAGA CCCAGAGTTT
    GAGTCCATCC TGGATATAGT GGATCCAAAC AGGGATGGAA ACGTGTCCTT GCAGGAGTAC
    ATGGCTTTCA TGATCAGCCG AGAAACGGAG AACGTCAAGT CAAGCGAGGA GATCGAGAGC
    GCTTTCCGTG CTCTGAGCGC AGAAAACAAA CCCTACGTCA CCAAGGAAGA ACTCTACCAG
    AACCTGACAA AGGAACAAGC CGACTACTGC ATTTCCCATA TGAAGCCCTA CCTAGACAGC
    AAGGGCCGCG AAATCCCATC AGCTTTCGAC TTTGTGGAAT TCACCCGCTC GCTCTTCGTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324501.1
    CDS22..7527
    Translation

    Target ORF information:

    RefSeq Version XM_021468826.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468826.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGCATA
    GCCTATCGGA GGGTAATCCG TGTCTATCAG TATGAAGTCG ATGATGATCT TTCTGGAAGG
    TCCTGCATGG TGGAAGAATC TGGAACTCTA GAGTCACAGC TAGAAGCCAC TAAGCGTAAG
    CATCAGGAGA TTCGTGCCAT GCGCAGTCAG CTGAAGAAGA TTGAGGATCT GGGAGCTGCC
    ATGGAGGAGG CGCTGATTCT GGACAATAAA TACACAGAAC ACAGCACCGT GGGTCTGGCA
    CAACAGTGGG ACCAACTCGA CCAGCTGGGC ATGAGAATGC AACATAACCT GGAACAACAG
    ATCCAAGCCA GAAACACTAC AGGTGTAACA GAAGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TCGACAAAGA AAAATCTGGC CGTCTGAATC ACCAGGAGTT CAAGTCTTGT
    CTGCGGTCTC TGGGTTACGA CCTTCCCATG GTGGAAGAAG GAGAACCAGA CCCAGAGTTT
    GAGTCCATCC TGGATATAGT GGATCCAAAC AGGGATGGAA ACGTGTCCTT GCAGGAGTAC
    ATGGCTTTCA TGATCAGCCG AGAAACGGAG AACGTCAAGT CAAGCGAGGA GATCGAGAGC
    GCTTTCCGTG CTCTGAGCGC AGAAAACAAA CCCTACGTCA CCAAGGAAGA ACTCTACCAG
    AACCTGACAA AGGAACAAGC CGACTACTGC ATTTCCCATA TGAAGCCCTA CCTAGACAGC
    AAGGGCCGCG AAATCCCATC AGCTTTCGAC TTTGTGGAAT TCACCCGCTC GCTCTTCGTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150886
    Clone ID Related Accession (Same CDS sequence) XM_021468825.1 , XM_021468826.1 , XM_021468824.1
    Accession Version XM_021468824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGCATA
    GCCTATCGGA GGGTAATCCG TGTCTATCAG TATGAAGTCG ATGATGATCT TTCTGGAAGG
    TCCTGCATGG TGGAAGAATC TGGAACTCTA GAGTCACAGC TAGAAGCCAC TAAGCGTAAG
    CATCAGGAGA TTCGTGCCAT GCGCAGTCAG CTGAAGAAGA TTGAGGATCT GGGAGCTGCC
    ATGGAGGAGG CGCTGATTCT GGACAATAAA TACACAGAAC ACAGCACCGT GGGTCTGGCA
    CAACAGTGGG ACCAACTCGA CCAGCTGGGC ATGAGAATGC AACATAACCT GGAACAACAG
    ATCCAAGCCA GAAACACTAC AGGTGTAACA GAAGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TCGACAAAGA AAAATCTGGC CGTCTGAATC ACCAGGAGTT CAAGTCTTGT
    CTGCGGTCTC TGGGTTACGA CCTTCCCATG GTGGAAGAAG GAGAACCAGA CCCAGAGTTT
    GAGTCCATCC TGGATATAGT GGATCCAAAC AGGGATGGAA ACGTGTCCTT GCAGGAGTAC
    ATGGCTTTCA TGATCAGCCG AGAAACGGAG AACGTCAAGT CAAGCGAGGA GATCGAGAGC
    GCTTTCCGTG CTCTGAGCGC AGAAAACAAA CCCTACGTCA CCAAGGAAGA ACTCTACCAG
    AACCTGACAA AGGAACAAGC CGACTACTGC ATTTCCCATA TGAAGCCCTA CCTAGACAGC
    AAGGGCCGCG AAATCCCATC AGCTTTCGAC TTTGTGGAAT TCACCCGCTC GCTCTTCGTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324499.1
    CDS136..7641
    Translation

    Target ORF information:

    RefSeq Version XM_021468824.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGCATA
    GCCTATCGGA GGGTAATCCG TGTCTATCAG TATGAAGTCG ATGATGATCT TTCTGGAAGG
    TCCTGCATGG TGGAAGAATC TGGAACTCTA GAGTCACAGC TAGAAGCCAC TAAGCGTAAG
    CATCAGGAGA TTCGTGCCAT GCGCAGTCAG CTGAAGAAGA TTGAGGATCT GGGAGCTGCC
    ATGGAGGAGG CGCTGATTCT GGACAATAAA TACACAGAAC ACAGCACCGT GGGTCTGGCA
    CAACAGTGGG ACCAACTCGA CCAGCTGGGC ATGAGAATGC AACATAACCT GGAACAACAG
    ATCCAAGCCA GAAACACTAC AGGTGTAACA GAAGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TCGACAAAGA AAAATCTGGC CGTCTGAATC ACCAGGAGTT CAAGTCTTGT
    CTGCGGTCTC TGGGTTACGA CCTTCCCATG GTGGAAGAAG GAGAACCAGA CCCAGAGTTT
    GAGTCCATCC TGGATATAGT GGATCCAAAC AGGGATGGAA ACGTGTCCTT GCAGGAGTAC
    ATGGCTTTCA TGATCAGCCG AGAAACGGAG AACGTCAAGT CAAGCGAGGA GATCGAGAGC
    GCTTTCCGTG CTCTGAGCGC AGAAAACAAA CCCTACGTCA CCAAGGAAGA ACTCTACCAG
    AACCTGACAA AGGAACAAGC CGACTACTGC ATTTCCCATA TGAAGCCCTA CCTAGACAGC
    AAGGGCCGCG AAATCCCATC AGCTTTCGAC TTTGTGGAAT TCACCCGCTC GCTCTTCGTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150887
    Clone ID Related Accession (Same CDS sequence) XM_021468827.1
    Accession Version XM_021468827.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGG ATGAAGCAGA CTCCAAAGGT
    GCATCTCCAT GGAAGTCTGT TCGCTTGGCT GTTCAAACAA CGGCCAACTT TAATACTATC
    AAGGAGCTGA ACAACCGCTG GAGGTCTCTG CAGCAGCTGG CTGAAGACCG AAGCAACATG
    CTGGGCAGTG CTCACGAAGT TCAGAGGTTC CACAGAGATG CAGATGAGAC CAAGGAGTGG
    ATCGAAGAGA AGAACCAAGC CCTGAACACT GATAACTATG GCCATGACCT CGCCAGCGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGATTT GAGAGAGATC TAGCCGCTTT AGGAGACAAG
    GTGAATTCTC TTGGTGAGAC TGCAGAGCGT CTGATTCAAT CTCATCCTGA GGCTGTGGAT
    GACATCCAGG AGAAGTGCAC CGAGCTAAAC ACAGCATGGA GCAGCCTGGT GGGTCGTGCA
    GACCAGCGCA AAGAAAAACT GGGCAACTCC CATGACCTGC AGCGCTTCCT CAGTGACTTC
    AGAGATCTCA TGTCTTGGAT CAATGGAATC AGGGGACTTG TGTCTTCCGA TGAGCTGGCC
    AAGGATGTTA CTGGTGCTGA AGCCCTGTTG GAGAGACATC AGGAACACCG CACTGAGATC
    GACGCTCGTG CAGGAACCTT CCTGGCTTTT GAGCAGTTTG GACAGCAGCT GCTCGCTCGT
    GGGCATTACG CAAGCCCTGA GATCAAACAG AAGCTGGAGG CTCTTGACCG GGAGCGTGCT
    GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ACCAGTGCCT GGAGCTGCAG
    TTGTTTAACC GTGACTGTGA GCAGGCTGAG AACTGGATGG CTGCCCGCGA AGCCTTCTTA
    GCCAGCGATG ACAAGGGAGA TTCTCTGGAC AGTGTGGAGG CGCTCATTAA GAAACACGAG
    GACTTTGATA AGGCTATCAA TGTGCAGGAA GAAAAGATTG CTGCACTGCA GTCTTTTGCT
    GACCAGCTCA TCAGCGCTGA CCACTATGCC AAGCCTGAGA TCTTCAAACG ACGCAGTGAG
    GTCTTGGACA GGTGGCTCCG CCTCAAGGCT CAGATGATTG AGAAACGCTC CAAGCTTGGC
    GAGTCACAGA CTCTACAGCA GTTCAGCAGA GACGTTGACG AGATCGAAGC TTGGATCAGT
    GAGAAACTCC AGACCGCCAC AGACGAGTCT TACAAAGACC CCACCAACAT CCAGCTGTCC
    AAGCTGCTGA GCAAACACCA GAAACACCAG GCTTTTGAGG CTGAGCTACA CGCTAACGCT
    GATCGTATCC GCGGAGTGAT TGACACGGGC AATGCTCTCA TCCAGAGAGG TGCCTGTGCT
    GGAAGTGAAG ATGCTGTTAA GGCTCGTCTG AATGCTCTGG ACGAGCAGTG GCAGTTCCTG
    GTCAACAAGT CTGCTGAGAA GAGTCAGAAA CTGAAGGAGG CCAACAAACA GCAGAACTTC
    AACACTGGCA TCAAGGACTT TGACTTCTGG TTGTCTGAGG TTGAGGCTCT TCTTGCCTCT
    GAGGATTATG GGAAAGATCT GGCTTCAGTC AACAATCTGC TGAAGAAGCA CCAGCTGTTG
    GAGGCTGATA TCTCTGCTCA TGAGGATCGT TTGAAGGATC TTAATGGCCA GGCTGACAGT
    CTGATGGCCA GCAACGCTTT CGACACATCT CAGGTCAAAG ACAAGCGGGA TGCCGTTAAC
    GGACGCTTCG GCAAAATCAA GAGCATGGCA GCGGGACGGC GAGCCAAGCT GAACGAATCG
    CATCGTCTGC ACCAGTTCTT CAGGGATCTG GACGATGAGG AGTCCTGGAT TAAGGAAAAG
    AAGTTGTTGG TGAGCTCGGA GGACTACGGA CGTGATTTGA CAGGGGTGCA GAATCTGAGG
    AAGAAGCACA AGAGGCTGGA GGCTGAGCTG GGAGCACACG AGCCGGCCAT TCAGTCTGTG
    TTGGAGACTG GGAAGAAGCT GTCTGACGAC AACACCATTG GACAGGAGGA GATCCAGCAG
    AGACTGGCCC AGTTTGTGGA GCACTGGAGG GAGCTGAAGG ATCTGGCTGC TGCACGTGGG
    CAGAGGCTTG AAGAGTCGCT GGAATACCAG CAGTTTGTGG CGAACGTGGA AGAAGAGGAA
    GCCTGGATCA ACGAGAAACT GAACCTGGTC GGAAGTGAAG ATTACGGTGA TACTCTGGCA
    GCTGTGCAGG GCTTGCTGAA GAAACATGAA GCATTTGAAA CTGATTTTAC TGTGCACAGG
    GACCGTGTGA ATGATGTGTG TTCCAATGGA GATGAACTCG TTAAAAAGGA GAACCACAAT
    GTGGAGAACA TCATGGCTAA AATGAAGGCG TTGCGTGGAA AAGTGTTGGA GCTGGAGAGA
    GCAGCGGCAC AGAGGAAAGC CAAGCTGGAT GAAAACTCTG CCTTCCTGCA GTTCAACTGG
    AAGGCTGATG TGGTGGAGTC CTGGATTGGT GAGAAGGAAA ACAGCCTGAA GACTGATGAC
    TATGGTCGCG ATCTCTCCTC TGTGCAGACG CTGCTCACCA AACAGGAAAC CTTTGATGCA
    GGACTTCAGG CCTTCCAGCA GGAGGGCATC ACCAACATCA CAGCTCTAAA AGACCAGCTG
    CTGGCAGCCA AACATGTGCA GTCTAAGGCC ATCGAGGCTC GTCACGCCAC CCTGATGAAG
    CGCTGGAACC AACTACTATC CAACTCAGCA GCCCGCAAGA AGAAGCTTCT GGAAGCACAG
    GACCACTTCA GAAAGGTGGA GGATCTTTTC CTCACGTTTG CCAAGAAAGC CTCAGCCTTC
    AACAGCTGGT TTGAGAACGC AGAGGAAGAT CTGACCGATC CTGTGAGGTG CAACTCTCTG
    GAGGAGATTA GGGCACTGCG GGATGCCCAT GATGCCTTCC GCTCGTCCCT GAGCTCCGCA
    GAGGCCGACT TCAGCCAGCT GGCAGAACTC GATCGGCAGA TTAAGAGCTA CAACGTGGTG
    TCCAACCCAT ACACCTGGTT CACCATGGAG GCTCTGGAGG AGACCTGGAG TAACCTTCAG
    AAGATCATCA AGGAGCGAGA ACTGGAGCTG CAGAAGGAGC AGCGCCGACA GGAAGAGAAT
    GATAAACTCA GACAGGAGTT TGCTCAGCAT GCTAATGCTT TCCACCAATG GCTGCAGGAG
    ACCAGGACAT ACCTTCTGGA TGGCATAGCC TATCGGAGGG TAATCCGTGT CTATCAGTAT
    GAAGTCGATG ATGATCTTTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG AACTCTAGAG
    TCACAGCTAG AAGCCACTAA GCGTAAGCAT CAGGAGATTC GTGCCATGCG CAGTCAGCTG
    AAGAAGATTG AGGATCTGGG AGCTGCCATG GAGGAGGCGC TGATTCTGGA CAATAAATAC
    ACAGAACACA GCACCGTGGG TCTGGCACAA CAGTGGGACC AACTCGACCA GCTGGGCATG
    AGAATGCAAC ATAACCTGGA ACAACAGATC CAAGCCAGAA ACACTACAGG TGTAACAGAA
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTCG ACAAAGAAAA ATCTGGCCGT
    CTGAATCACC AGGAGTTCAA GTCTTGTCTG CGGTCTCTGG GTTACGACCT TCCCATGGTG
    GAAGAAGGAG AACCAGACCC AGAGTTTGAG TCCATCCTGG ATATAGTGGA TCCAAACAGG
    GATGGAAACG TGTCCTTGCA GGAGTACATG GCTTTCATGA TCAGCCGAGA AACGGAGAAC
    GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT TTCCGTGCTC TGAGCGCAGA AAACAAACCC
    TACGTCACCA AGGAAGAACT CTACCAGAAC CTGACAAAGG AACAAGCCGA CTACTGCATT
    TCCCATATGA AGCCCTACCT AGACAGCAAG GGCCGCGAAA TCCCATCAGC TTTCGACTTT
    GTGGAATTCA CCCGCTCGCT CTTCGTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324502.1
    CDS4..7476
    Translation

    Target ORF information:

    RefSeq Version XM_021468827.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468827.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGG ATGAAGCAGA CTCCAAAGGT
    GCATCTCCAT GGAAGTCTGT TCGCTTGGCT GTTCAAACAA CGGCCAACTT TAATACTATC
    AAGGAGCTGA ACAACCGCTG GAGGTCTCTG CAGCAGCTGG CTGAAGACCG AAGCAACATG
    CTGGGCAGTG CTCACGAAGT TCAGAGGTTC CACAGAGATG CAGATGAGAC CAAGGAGTGG
    ATCGAAGAGA AGAACCAAGC CCTGAACACT GATAACTATG GCCATGACCT CGCCAGCGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGATTT GAGAGAGATC TAGCCGCTTT AGGAGACAAG
    GTGAATTCTC TTGGTGAGAC TGCAGAGCGT CTGATTCAAT CTCATCCTGA GGCTGTGGAT
    GACATCCAGG AGAAGTGCAC CGAGCTAAAC ACAGCATGGA GCAGCCTGGT GGGTCGTGCA
    GACCAGCGCA AAGAAAAACT GGGCAACTCC CATGACCTGC AGCGCTTCCT CAGTGACTTC
    AGAGATCTCA TGTCTTGGAT CAATGGAATC AGGGGACTTG TGTCTTCCGA TGAGCTGGCC
    AAGGATGTTA CTGGTGCTGA AGCCCTGTTG GAGAGACATC AGGAACACCG CACTGAGATC
    GACGCTCGTG CAGGAACCTT CCTGGCTTTT GAGCAGTTTG GACAGCAGCT GCTCGCTCGT
    GGGCATTACG CAAGCCCTGA GATCAAACAG AAGCTGGAGG CTCTTGACCG GGAGCGTGCT
    GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ACCAGTGCCT GGAGCTGCAG
    TTGTTTAACC GTGACTGTGA GCAGGCTGAG AACTGGATGG CTGCCCGCGA AGCCTTCTTA
    GCCAGCGATG ACAAGGGAGA TTCTCTGGAC AGTGTGGAGG CGCTCATTAA GAAACACGAG
    GACTTTGATA AGGCTATCAA TGTGCAGGAA GAAAAGATTG CTGCACTGCA GTCTTTTGCT
    GACCAGCTCA TCAGCGCTGA CCACTATGCC AAGCCTGAGA TCTTCAAACG ACGCAGTGAG
    GTCTTGGACA GGTGGCTCCG CCTCAAGGCT CAGATGATTG AGAAACGCTC CAAGCTTGGC
    GAGTCACAGA CTCTACAGCA GTTCAGCAGA GACGTTGACG AGATCGAAGC TTGGATCAGT
    GAGAAACTCC AGACCGCCAC AGACGAGTCT TACAAAGACC CCACCAACAT CCAGCTGTCC
    AAGCTGCTGA GCAAACACCA GAAACACCAG GCTTTTGAGG CTGAGCTACA CGCTAACGCT
    GATCGTATCC GCGGAGTGAT TGACACGGGC AATGCTCTCA TCCAGAGAGG TGCCTGTGCT
    GGAAGTGAAG ATGCTGTTAA GGCTCGTCTG AATGCTCTGG ACGAGCAGTG GCAGTTCCTG
    GTCAACAAGT CTGCTGAGAA GAGTCAGAAA CTGAAGGAGG CCAACAAACA GCAGAACTTC
    AACACTGGCA TCAAGGACTT TGACTTCTGG TTGTCTGAGG TTGAGGCTCT TCTTGCCTCT
    GAGGATTATG GGAAAGATCT GGCTTCAGTC AACAATCTGC TGAAGAAGCA CCAGCTGTTG
    GAGGCTGATA TCTCTGCTCA TGAGGATCGT TTGAAGGATC TTAATGGCCA GGCTGACAGT
    CTGATGGCCA GCAACGCTTT CGACACATCT CAGGTCAAAG ACAAGCGGGA TGCCGTTAAC
    GGACGCTTCG GCAAAATCAA GAGCATGGCA GCGGGACGGC GAGCCAAGCT GAACGAATCG
    CATCGTCTGC ACCAGTTCTT CAGGGATCTG GACGATGAGG AGTCCTGGAT TAAGGAAAAG
    AAGTTGTTGG TGAGCTCGGA GGACTACGGA CGTGATTTGA CAGGGGTGCA GAATCTGAGG
    AAGAAGCACA AGAGGCTGGA GGCTGAGCTG GGAGCACACG AGCCGGCCAT TCAGTCTGTG
    TTGGAGACTG GGAAGAAGCT GTCTGACGAC AACACCATTG GACAGGAGGA GATCCAGCAG
    AGACTGGCCC AGTTTGTGGA GCACTGGAGG GAGCTGAAGG ATCTGGCTGC TGCACGTGGG
    CAGAGGCTTG AAGAGTCGCT GGAATACCAG CAGTTTGTGG CGAACGTGGA AGAAGAGGAA
    GCCTGGATCA ACGAGAAACT GAACCTGGTC GGAAGTGAAG ATTACGGTGA TACTCTGGCA
    GCTGTGCAGG GCTTGCTGAA GAAACATGAA GCATTTGAAA CTGATTTTAC TGTGCACAGG
    GACCGTGTGA ATGATGTGTG TTCCAATGGA GATGAACTCG TTAAAAAGGA GAACCACAAT
    GTGGAGAACA TCATGGCTAA AATGAAGGCG TTGCGTGGAA AAGTGTTGGA GCTGGAGAGA
    GCAGCGGCAC AGAGGAAAGC CAAGCTGGAT GAAAACTCTG CCTTCCTGCA GTTCAACTGG
    AAGGCTGATG TGGTGGAGTC CTGGATTGGT GAGAAGGAAA ACAGCCTGAA GACTGATGAC
    TATGGTCGCG ATCTCTCCTC TGTGCAGACG CTGCTCACCA AACAGGAAAC CTTTGATGCA
    GGACTTCAGG CCTTCCAGCA GGAGGGCATC ACCAACATCA CAGCTCTAAA AGACCAGCTG
    CTGGCAGCCA AACATGTGCA GTCTAAGGCC ATCGAGGCTC GTCACGCCAC CCTGATGAAG
    CGCTGGAACC AACTACTATC CAACTCAGCA GCCCGCAAGA AGAAGCTTCT GGAAGCACAG
    GACCACTTCA GAAAGGTGGA GGATCTTTTC CTCACGTTTG CCAAGAAAGC CTCAGCCTTC
    AACAGCTGGT TTGAGAACGC AGAGGAAGAT CTGACCGATC CTGTGAGGTG CAACTCTCTG
    GAGGAGATTA GGGCACTGCG GGATGCCCAT GATGCCTTCC GCTCGTCCCT GAGCTCCGCA
    GAGGCCGACT TCAGCCAGCT GGCAGAACTC GATCGGCAGA TTAAGAGCTA CAACGTGGTG
    TCCAACCCAT ACACCTGGTT CACCATGGAG GCTCTGGAGG AGACCTGGAG TAACCTTCAG
    AAGATCATCA AGGAGCGAGA ACTGGAGCTG CAGAAGGAGC AGCGCCGACA GGAAGAGAAT
    GATAAACTCA GACAGGAGTT TGCTCAGCAT GCTAATGCTT TCCACCAATG GCTGCAGGAG
    ACCAGGACAT ACCTTCTGGA TGGCATAGCC TATCGGAGGG TAATCCGTGT CTATCAGTAT
    GAAGTCGATG ATGATCTTTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG AACTCTAGAG
    TCACAGCTAG AAGCCACTAA GCGTAAGCAT CAGGAGATTC GTGCCATGCG CAGTCAGCTG
    AAGAAGATTG AGGATCTGGG AGCTGCCATG GAGGAGGCGC TGATTCTGGA CAATAAATAC
    ACAGAACACA GCACCGTGGG TCTGGCACAA CAGTGGGACC AACTCGACCA GCTGGGCATG
    AGAATGCAAC ATAACCTGGA ACAACAGATC CAAGCCAGAA ACACTACAGG TGTAACAGAA
    GAGGCCCTGA AGGAGTTCAG CATGATGTTC AAGCACTTCG ACAAAGAAAA ATCTGGCCGT
    CTGAATCACC AGGAGTTCAA GTCTTGTCTG CGGTCTCTGG GTTACGACCT TCCCATGGTG
    GAAGAAGGAG AACCAGACCC AGAGTTTGAG TCCATCCTGG ATATAGTGGA TCCAAACAGG
    GATGGAAACG TGTCCTTGCA GGAGTACATG GCTTTCATGA TCAGCCGAGA AACGGAGAAC
    GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT TTCCGTGCTC TGAGCGCAGA AAACAAACCC
    TACGTCACCA AGGAAGAACT CTACCAGAAC CTGACAAAGG AACAAGCCGA CTACTGCATT
    TCCCATATGA AGCCCTACCT AGACAGCAAG GGCCGCGAAA TCCCATCAGC TTTCGACTTT
    GTGGAATTCA CCCGCTCGCT CTTCGTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150888
    Clone ID Related Accession (Same CDS sequence) XM_021468828.1
    Accession Version XM_021468828.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGGA GCTGAACAAC
    CGCTGGAGGT CTCTGCAGCA GCTGGCTGAA GACCGAAGCA ACATGCTGGG CAGTGCTCAC
    GAAGTTCAGA GGTTCCACAG AGATGCAGAT GAGACCAAGG AGTGGATCGA AGAGAAGAAC
    CAAGCCCTGA ACACTGATAA CTATGGCCAT GACCTCGCCA GCGTGCAGGC TCTGCAGCGC
    AAGCATGAAG GATTTGAGAG AGATCTAGCC GCTTTAGGAG ACAAGGTGAA TTCTCTTGGT
    GAGACTGCAG AGCGTCTGAT TCAATCTCAT CCTGAGGCTG TGGATGACAT CCAGGAGAAG
    TGCACCGAGC TAAACACAGC ATGGAGCAGC CTGGTGGGTC GTGCAGACCA GCGCAAAGAA
    AAACTGGGCA ACTCCCATGA CCTGCAGCGC TTCCTCAGTG ACTTCAGAGA TCTCATGTCT
    TGGATCAATG GAATCAGGGG ACTTGTGTCT TCCGATGAGC TGGCCAAGGA TGTTACTGGT
    GCTGAAGCCC TGTTGGAGAG ACATCAGGAA CACCGCACTG AGATCGACGC TCGTGCAGGA
    ACCTTCCTGG CTTTTGAGCA GTTTGGACAG CAGCTGCTCG CTCGTGGGCA TTACGCAAGC
    CCTGAGATCA AACAGAAGCT GGAGGCTCTT GACCGGGAGC GTGCTGACCT GGAGAAGGCC
    TGGGTTCAGC GCAGGATGAT GCTGGACCAG TGCCTGGAGC TGCAGTTGTT TAACCGTGAC
    TGTGAGCAGG CTGAGAACTG GATGGCTGCC CGCGAAGCCT TCTTAGCCAG CGATGACAAG
    GGAGATTCTC TGGACAGTGT GGAGGCGCTC ATTAAGAAAC ACGAGGACTT TGATAAGGCT
    ATCAATGTGC AGGAAGAAAA GATTGCTGCA CTGCAGTCTT TTGCTGACCA GCTCATCAGC
    GCTGACCACT ATGCCAAGCC TGAGATCTTC AAACGACGCA GTGAGGTCTT GGACAGGTGG
    CTCCGCCTCA AGGCTCAGAT GATTGAGAAA CGCTCCAAGC TTGGCGAGTC ACAGACTCTA
    CAGCAGTTCA GCAGAGACGT TGACGAGATC GAAGCTTGGA TCAGTGAGAA ACTCCAGACC
    GCCACAGACG AGTCTTACAA AGACCCCACC AACATCCAGC TGTCCAAGCT GCTGAGCAAA
    CACCAGAAAC ACCAGGCTTT TGAGGCTGAG CTACACGCTA ACGCTGATCG TATCCGCGGA
    GTGATTGACA CGGGCAATGC TCTCATCCAG AGAGGTGCCT GTGCTGGAAG TGAAGATGCT
    GTTAAGGCTC GTCTGAATGC TCTGGACGAG CAGTGGCAGT TCCTGGTCAA CAAGTCTGCT
    GAGAAGAGTC AGAAACTGAA GGAGGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG
    GACTTTGACT TCTGGTTGTC TGAGGTTGAG GCTCTTCTTG CCTCTGAGGA TTATGGGAAA
    GATCTGGCTT CAGTCAACAA TCTGCTGAAG AAGCACCAGC TGTTGGAGGC TGATATCTCT
    GCTCATGAGG ATCGTTTGAA GGATCTTAAT GGCCAGGCTG ACAGTCTGAT GGCCAGCAAC
    GCTTTCGACA CATCTCAGGT CAAAGACAAG CGGGATGCCG TTAACGGACG CTTCGGCAAA
    ATCAAGAGCA TGGCAGCGGG ACGGCGAGCC AAGCTGAACG AATCGCATCG TCTGCACCAG
    TTCTTCAGGG ATCTGGACGA TGAGGAGTCC TGGATTAAGG AAAAGAAGTT GTTGGTGAGC
    TCGGAGGACT ACGGACGTGA TTTGACAGGG GTGCAGAATC TGAGGAAGAA GCACAAGAGG
    CTGGAGGCTG AGCTGGGAGC ACACGAGCCG GCCATTCAGT CTGTGTTGGA GACTGGGAAG
    AAGCTGTCTG ACGACAACAC CATTGGACAG GAGGAGATCC AGCAGAGACT GGCCCAGTTT
    GTGGAGCACT GGAGGGAGCT GAAGGATCTG GCTGCTGCAC GTGGGCAGAG GCTTGAAGAG
    TCGCTGGAAT ACCAGCAGTT TGTGGCGAAC GTGGAAGAAG AGGAAGCCTG GATCAACGAG
    AAACTGAACC TGGTCGGAAG TGAAGATTAC GGTGATACTC TGGCAGCTGT GCAGGGCTTG
    CTGAAGAAAC ATGAAGCATT TGAAACTGAT TTTACTGTGC ACAGGGACCG TGTGAATGAT
    GTGTGTTCCA ATGGAGATGA ACTCGTTAAA AAGGAGAACC ACAATGTGGA GAACATCATG
    GCTAAAATGA AGGCGTTGCG TGGAAAAGTG TTGGAGCTGG AGAGAGCAGC GGCACAGAGG
    AAAGCCAAGC TGGATGAAAA CTCTGCCTTC CTGCAGTTCA ACTGGAAGGC TGATGTGGTG
    GAGTCCTGGA TTGGTGAGAA GGAAAACAGC CTGAAGACTG ATGACTATGG TCGCGATCTC
    TCCTCTGTGC AGACGCTGCT CACCAAACAG GAAACCTTTG ATGCAGGACT TCAGGCCTTC
    CAGCAGGAGG GCATCACCAA CATCACAGCT CTAAAAGACC AGCTGCTGGC AGCCAAACAT
    GTGCAGTCTA AGGCCATCGA GGCTCGTCAC GCCACCCTGA TGAAGCGCTG GAACCAACTA
    CTATCCAACT CAGCAGCCCG CAAGAAGAAG CTTCTGGAAG CACAGGACCA CTTCAGAAAG
    GTGGAGGATC TTTTCCTCAC GTTTGCCAAG AAAGCCTCAG CCTTCAACAG CTGGTTTGAG
    AACGCAGAGG AAGATCTGAC CGATCCTGTG AGGTGCAACT CTCTGGAGGA GATTAGGGCA
    CTGCGGGATG CCCATGATGC CTTCCGCTCG TCCCTGAGCT CCGCAGAGGC CGACTTCAGC
    CAGCTGGCAG AACTCGATCG GCAGATTAAG AGCTACAACG TGGTGTCCAA CCCATACACC
    TGGTTCACCA TGGAGGCTCT GGAGGAGACC TGGAGTAACC TTCAGAAGAT CATCAAGGAG
    CGAGAACTGG AGCTGCAGAA GGAGCAGCGC CGACAGGAAG AGAATGATAA ACTCAGACAG
    GAGTTTGCTC AGCATGCTAA TGCTTTCCAC CAATGGCTGC AGGAGACCAG GACATACCTT
    CTGGATGGCA TAGCCTATCG GAGGGTAATC CGTGTCTATC AGTATGAAGT CGATGATGAT
    CTTTCTGGAA GGTCCTGCAT GGTGGAAGAA TCTGGAACTC TAGAGTCACA GCTAGAAGCC
    ACTAAGCGTA AGCATCAGGA GATTCGTGCC ATGCGCAGTC AGCTGAAGAA GATTGAGGAT
    CTGGGAGCTG CCATGGAGGA GGCGCTGATT CTGGACAATA AATACACAGA ACACAGCACC
    GTGGGTCTGG CACAACAGTG GGACCAACTC GACCAGCTGG GCATGAGAAT GCAACATAAC
    CTGGAACAAC AGATCCAAGC CAGAAACACT ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG
    TTCAGCATGA TGTTCAAGCA CTTCGACAAA GAAAAATCTG GCCGTCTGAA TCACCAGGAG
    TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC GACCTTCCCA TGGTGGAAGA AGGAGAACCA
    GACCCAGAGT TTGAGTCCAT CCTGGATATA GTGGATCCAA ACAGGGATGG AAACGTGTCC
    TTGCAGGAGT ACATGGCTTT CATGATCAGC CGAGAAACGG AGAACGTCAA GTCAAGCGAG
    GAGATCGAGA GCGCTTTCCG TGCTCTGAGC GCAGAAAACA AACCCTACGT CACCAAGGAA
    GAACTCTACC AGAACCTGAC AAAGGAACAA GCCGACTACT GCATTTCCCA TATGAAGCCC
    TACCTAGACA GCAAGGGCCG CGAAATCCCA TCAGCTTTCG ACTTTGTGGA ATTCACCCGC
    TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324503.1
    CDS4..7461
    Translation

    Target ORF information:

    RefSeq Version XM_021468828.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468828.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGGA GCTGAACAAC
    CGCTGGAGGT CTCTGCAGCA GCTGGCTGAA GACCGAAGCA ACATGCTGGG CAGTGCTCAC
    GAAGTTCAGA GGTTCCACAG AGATGCAGAT GAGACCAAGG AGTGGATCGA AGAGAAGAAC
    CAAGCCCTGA ACACTGATAA CTATGGCCAT GACCTCGCCA GCGTGCAGGC TCTGCAGCGC
    AAGCATGAAG GATTTGAGAG AGATCTAGCC GCTTTAGGAG ACAAGGTGAA TTCTCTTGGT
    GAGACTGCAG AGCGTCTGAT TCAATCTCAT CCTGAGGCTG TGGATGACAT CCAGGAGAAG
    TGCACCGAGC TAAACACAGC ATGGAGCAGC CTGGTGGGTC GTGCAGACCA GCGCAAAGAA
    AAACTGGGCA ACTCCCATGA CCTGCAGCGC TTCCTCAGTG ACTTCAGAGA TCTCATGTCT
    TGGATCAATG GAATCAGGGG ACTTGTGTCT TCCGATGAGC TGGCCAAGGA TGTTACTGGT
    GCTGAAGCCC TGTTGGAGAG ACATCAGGAA CACCGCACTG AGATCGACGC TCGTGCAGGA
    ACCTTCCTGG CTTTTGAGCA GTTTGGACAG CAGCTGCTCG CTCGTGGGCA TTACGCAAGC
    CCTGAGATCA AACAGAAGCT GGAGGCTCTT GACCGGGAGC GTGCTGACCT GGAGAAGGCC
    TGGGTTCAGC GCAGGATGAT GCTGGACCAG TGCCTGGAGC TGCAGTTGTT TAACCGTGAC
    TGTGAGCAGG CTGAGAACTG GATGGCTGCC CGCGAAGCCT TCTTAGCCAG CGATGACAAG
    GGAGATTCTC TGGACAGTGT GGAGGCGCTC ATTAAGAAAC ACGAGGACTT TGATAAGGCT
    ATCAATGTGC AGGAAGAAAA GATTGCTGCA CTGCAGTCTT TTGCTGACCA GCTCATCAGC
    GCTGACCACT ATGCCAAGCC TGAGATCTTC AAACGACGCA GTGAGGTCTT GGACAGGTGG
    CTCCGCCTCA AGGCTCAGAT GATTGAGAAA CGCTCCAAGC TTGGCGAGTC ACAGACTCTA
    CAGCAGTTCA GCAGAGACGT TGACGAGATC GAAGCTTGGA TCAGTGAGAA ACTCCAGACC
    GCCACAGACG AGTCTTACAA AGACCCCACC AACATCCAGC TGTCCAAGCT GCTGAGCAAA
    CACCAGAAAC ACCAGGCTTT TGAGGCTGAG CTACACGCTA ACGCTGATCG TATCCGCGGA
    GTGATTGACA CGGGCAATGC TCTCATCCAG AGAGGTGCCT GTGCTGGAAG TGAAGATGCT
    GTTAAGGCTC GTCTGAATGC TCTGGACGAG CAGTGGCAGT TCCTGGTCAA CAAGTCTGCT
    GAGAAGAGTC AGAAACTGAA GGAGGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG
    GACTTTGACT TCTGGTTGTC TGAGGTTGAG GCTCTTCTTG CCTCTGAGGA TTATGGGAAA
    GATCTGGCTT CAGTCAACAA TCTGCTGAAG AAGCACCAGC TGTTGGAGGC TGATATCTCT
    GCTCATGAGG ATCGTTTGAA GGATCTTAAT GGCCAGGCTG ACAGTCTGAT GGCCAGCAAC
    GCTTTCGACA CATCTCAGGT CAAAGACAAG CGGGATGCCG TTAACGGACG CTTCGGCAAA
    ATCAAGAGCA TGGCAGCGGG ACGGCGAGCC AAGCTGAACG AATCGCATCG TCTGCACCAG
    TTCTTCAGGG ATCTGGACGA TGAGGAGTCC TGGATTAAGG AAAAGAAGTT GTTGGTGAGC
    TCGGAGGACT ACGGACGTGA TTTGACAGGG GTGCAGAATC TGAGGAAGAA GCACAAGAGG
    CTGGAGGCTG AGCTGGGAGC ACACGAGCCG GCCATTCAGT CTGTGTTGGA GACTGGGAAG
    AAGCTGTCTG ACGACAACAC CATTGGACAG GAGGAGATCC AGCAGAGACT GGCCCAGTTT
    GTGGAGCACT GGAGGGAGCT GAAGGATCTG GCTGCTGCAC GTGGGCAGAG GCTTGAAGAG
    TCGCTGGAAT ACCAGCAGTT TGTGGCGAAC GTGGAAGAAG AGGAAGCCTG GATCAACGAG
    AAACTGAACC TGGTCGGAAG TGAAGATTAC GGTGATACTC TGGCAGCTGT GCAGGGCTTG
    CTGAAGAAAC ATGAAGCATT TGAAACTGAT TTTACTGTGC ACAGGGACCG TGTGAATGAT
    GTGTGTTCCA ATGGAGATGA ACTCGTTAAA AAGGAGAACC ACAATGTGGA GAACATCATG
    GCTAAAATGA AGGCGTTGCG TGGAAAAGTG TTGGAGCTGG AGAGAGCAGC GGCACAGAGG
    AAAGCCAAGC TGGATGAAAA CTCTGCCTTC CTGCAGTTCA ACTGGAAGGC TGATGTGGTG
    GAGTCCTGGA TTGGTGAGAA GGAAAACAGC CTGAAGACTG ATGACTATGG TCGCGATCTC
    TCCTCTGTGC AGACGCTGCT CACCAAACAG GAAACCTTTG ATGCAGGACT TCAGGCCTTC
    CAGCAGGAGG GCATCACCAA CATCACAGCT CTAAAAGACC AGCTGCTGGC AGCCAAACAT
    GTGCAGTCTA AGGCCATCGA GGCTCGTCAC GCCACCCTGA TGAAGCGCTG GAACCAACTA
    CTATCCAACT CAGCAGCCCG CAAGAAGAAG CTTCTGGAAG CACAGGACCA CTTCAGAAAG
    GTGGAGGATC TTTTCCTCAC GTTTGCCAAG AAAGCCTCAG CCTTCAACAG CTGGTTTGAG
    AACGCAGAGG AAGATCTGAC CGATCCTGTG AGGTGCAACT CTCTGGAGGA GATTAGGGCA
    CTGCGGGATG CCCATGATGC CTTCCGCTCG TCCCTGAGCT CCGCAGAGGC CGACTTCAGC
    CAGCTGGCAG AACTCGATCG GCAGATTAAG AGCTACAACG TGGTGTCCAA CCCATACACC
    TGGTTCACCA TGGAGGCTCT GGAGGAGACC TGGAGTAACC TTCAGAAGAT CATCAAGGAG
    CGAGAACTGG AGCTGCAGAA GGAGCAGCGC CGACAGGAAG AGAATGATAA ACTCAGACAG
    GAGTTTGCTC AGCATGCTAA TGCTTTCCAC CAATGGCTGC AGGAGACCAG GACATACCTT
    CTGGATGGCA TAGCCTATCG GAGGGTAATC CGTGTCTATC AGTATGAAGT CGATGATGAT
    CTTTCTGGAA GGTCCTGCAT GGTGGAAGAA TCTGGAACTC TAGAGTCACA GCTAGAAGCC
    ACTAAGCGTA AGCATCAGGA GATTCGTGCC ATGCGCAGTC AGCTGAAGAA GATTGAGGAT
    CTGGGAGCTG CCATGGAGGA GGCGCTGATT CTGGACAATA AATACACAGA ACACAGCACC
    GTGGGTCTGG CACAACAGTG GGACCAACTC GACCAGCTGG GCATGAGAAT GCAACATAAC
    CTGGAACAAC AGATCCAAGC CAGAAACACT ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG
    TTCAGCATGA TGTTCAAGCA CTTCGACAAA GAAAAATCTG GCCGTCTGAA TCACCAGGAG
    TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC GACCTTCCCA TGGTGGAAGA AGGAGAACCA
    GACCCAGAGT TTGAGTCCAT CCTGGATATA GTGGATCCAA ACAGGGATGG AAACGTGTCC
    TTGCAGGAGT ACATGGCTTT CATGATCAGC CGAGAAACGG AGAACGTCAA GTCAAGCGAG
    GAGATCGAGA GCGCTTTCCG TGCTCTGAGC GCAGAAAACA AACCCTACGT CACCAAGGAA
    GAACTCTACC AGAACCTGAC AAAGGAACAA GCCGACTACT GCATTTCCCA TATGAAGCCC
    TACCTAGACA GCAAGGGCCG CGAAATCCCA TCAGCTTTCG ACTTTGTGGA ATTCACCCGC
    TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150889
    Clone ID Related Accession (Same CDS sequence) XM_021468829.1
    Accession Version XM_021468829.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    481
    541
    601
    661
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    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGCATA
    GCCTATCGGA GGGTAATCCG TGTCTATCAG TATGAAGTCG ATGATGATCT TTCTGGAAGG
    TCCTGCATGG TGGAAGAATC TGGAACTCTA GAGTCACAGC TAGAAGCCAC TAAGCGTAAG
    CATCAGGAGA TTCGTGCCAT GCGCAGTCAG CTGAAGAAGA TTGAGGATCT GGGAGCTGCC
    ATGGAGGAGG CGCTGATTCT GGACAATAAA TACACAGAAC ACAGCACCGT GGGTCTGGCA
    CAACAGTGGG ACCAACTCGA CCAGCTGGGC ATGAGAATGC AACATAACCT GGAACAACAG
    ATCCAAGCCA GAAACACTAC AGGTGTAACA GAAGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TCGACAAAGA AAAATCTGGC CGTCTGAATC ACCAGGAGTT CAAGTCTTGT
    CTGCGGTCTC TGGGTTACGA CCTTCCCATG GTGGAAGAAG GAGAACCAGA CCCAGAGTTT
    GAGTCCATCC TGGATATAGT GGATCCAAAC AGGGATGGAA ACGTGTCCTT GCAGGAGTAC
    ATGGCTTTCA TGATCAGCCG AGAAACGGAG AACGTCAAGT CAAGCGAGGA GATCGAGAGC
    GCTTTCCGTG CTCTGAGCGC AGAAAACAAA CCCTACGTCA CCAAGGAAGA ACTCTACCAG
    AACCTGACAA AGGAACAAGC CGACTACTGC ATTTCCCATA TGAAGCCCTA CCTAGACAGC
    AAGGGCCGCG AAATCCCATC AGCTTTCGAC TTTGTGGAAT TCACCCGCTC GCTCTTCGTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324504.1
    CDS4..7449
    Translation

    Target ORF information:

    RefSeq Version XM_021468829.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468829.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGCATA
    GCCTATCGGA GGGTAATCCG TGTCTATCAG TATGAAGTCG ATGATGATCT TTCTGGAAGG
    TCCTGCATGG TGGAAGAATC TGGAACTCTA GAGTCACAGC TAGAAGCCAC TAAGCGTAAG
    CATCAGGAGA TTCGTGCCAT GCGCAGTCAG CTGAAGAAGA TTGAGGATCT GGGAGCTGCC
    ATGGAGGAGG CGCTGATTCT GGACAATAAA TACACAGAAC ACAGCACCGT GGGTCTGGCA
    CAACAGTGGG ACCAACTCGA CCAGCTGGGC ATGAGAATGC AACATAACCT GGAACAACAG
    ATCCAAGCCA GAAACACTAC AGGTGTAACA GAAGAGGCCC TGAAGGAGTT CAGCATGATG
    TTCAAGCACT TCGACAAAGA AAAATCTGGC CGTCTGAATC ACCAGGAGTT CAAGTCTTGT
    CTGCGGTCTC TGGGTTACGA CCTTCCCATG GTGGAAGAAG GAGAACCAGA CCCAGAGTTT
    GAGTCCATCC TGGATATAGT GGATCCAAAC AGGGATGGAA ACGTGTCCTT GCAGGAGTAC
    ATGGCTTTCA TGATCAGCCG AGAAACGGAG AACGTCAAGT CAAGCGAGGA GATCGAGAGC
    GCTTTCCGTG CTCTGAGCGC AGAAAACAAA CCCTACGTCA CCAAGGAAGA ACTCTACCAG
    AACCTGACAA AGGAACAAGC CGACTACTGC ATTTCCCATA TGAAGCCCTA CCTAGACAGC
    AAGGGCCGCG AAATCCCATC AGCTTTCGAC TTTGTGGAAT TCACCCGCTC GCTCTTCGTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150890
    Clone ID Related Accession (Same CDS sequence) XM_021468832.1
    Accession Version XM_021468832.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGG ATGAAGCAGA CTCCAAAGGT
    GCATCTCCAT GGAAGGAGCT GAACAACCGC TGGAGGTCTC TGCAGCAGCT GGCTGAAGAC
    CGAAGCAACA TGCTGGGCAG TGCTCACGAA GTTCAGAGGT TCCACAGAGA TGCAGATGAG
    ACCAAGGAGT GGATCGAAGA GAAGAACCAA GCCCTGAACA CTGATAACTA TGGCCATGAC
    CTCGCCAGCG TGCAGGCTCT GCAGCGCAAG CATGAAGGAT TTGAGAGAGA TCTAGCCGCT
    TTAGGAGACA AGGTGAATTC TCTTGGTGAG ACTGCAGAGC GTCTGATTCA ATCTCATCCT
    GAGGCTGTGG ATGACATCCA GGAGAAGTGC ACCGAGCTAA ACACAGCATG GAGCAGCCTG
    GTGGGTCGTG CAGACCAGCG CAAAGAAAAA CTGGGCAACT CCCATGACCT GCAGCGCTTC
    CTCAGTGACT TCAGAGATCT CATGTCTTGG ATCAATGGAA TCAGGGGACT TGTGTCTTCC
    GATGAGCTGG CCAAGGATGT TACTGGTGCT GAAGCCCTGT TGGAGAGACA TCAGGAACAC
    CGCACTGAGA TCGACGCTCG TGCAGGAACC TTCCTGGCTT TTGAGCAGTT TGGACAGCAG
    CTGCTCGCTC GTGGGCATTA CGCAAGCCCT GAGATCAAAC AGAAGCTGGA GGCTCTTGAC
    CGGGAGCGTG CTGACCTGGA GAAGGCCTGG GTTCAGCGCA GGATGATGCT GGACCAGTGC
    CTGGAGCTGC AGTTGTTTAA CCGTGACTGT GAGCAGGCTG AGAACTGGAT GGCTGCCCGC
    GAAGCCTTCT TAGCCAGCGA TGACAAGGGA GATTCTCTGG ACAGTGTGGA GGCGCTCATT
    AAGAAACACG AGGACTTTGA TAAGGCTATC AATGTGCAGG AAGAAAAGAT TGCTGCACTG
    CAGTCTTTTG CTGACCAGCT CATCAGCGCT GACCACTATG CCAAGCCTGA GATCTTCAAA
    CGACGCAGTG AGGTCTTGGA CAGGTGGCTC CGCCTCAAGG CTCAGATGAT TGAGAAACGC
    TCCAAGCTTG GCGAGTCACA GACTCTACAG CAGTTCAGCA GAGACGTTGA CGAGATCGAA
    GCTTGGATCA GTGAGAAACT CCAGACCGCC ACAGACGAGT CTTACAAAGA CCCCACCAAC
    ATCCAGCTGT CCAAGCTGCT GAGCAAACAC CAGAAACACC AGGCTTTTGA GGCTGAGCTA
    CACGCTAACG CTGATCGTAT CCGCGGAGTG ATTGACACGG GCAATGCTCT CATCCAGAGA
    GGTGCCTGTG CTGGAAGTGA AGATGCTGTT AAGGCTCGTC TGAATGCTCT GGACGAGCAG
    TGGCAGTTCC TGGTCAACAA GTCTGCTGAG AAGAGTCAGA AACTGAAGGA GGCCAACAAA
    CAGCAGAACT TCAACACTGG CATCAAGGAC TTTGACTTCT GGTTGTCTGA GGTTGAGGCT
    CTTCTTGCCT CTGAGGATTA TGGGAAAGAT CTGGCTTCAG TCAACAATCT GCTGAAGAAG
    CACCAGCTGT TGGAGGCTGA TATCTCTGCT CATGAGGATC GTTTGAAGGA TCTTAATGGC
    CAGGCTGACA GTCTGATGGC CAGCAACGCT TTCGACACAT CTCAGGTCAA AGACAAGCGG
    GATGCCGTTA ACGGACGCTT CGGCAAAATC AAGAGCATGG CAGCGGGACG GCGAGCCAAG
    CTGAACGAAT CGCATCGTCT GCACCAGTTC TTCAGGGATC TGGACGATGA GGAGTCCTGG
    ATTAAGGAAA AGAAGTTGTT GGTGAGCTCG GAGGACTACG GACGTGATTT GACAGGGGTG
    CAGAATCTGA GGAAGAAGCA CAAGAGGCTG GAGGCTGAGC TGGGAGCACA CGAGCCGGCC
    ATTCAGTCTG TGTTGGAGAC TGGGAAGAAG CTGTCTGACG ACAACACCAT TGGACAGGAG
    GAGATCCAGC AGAGACTGGC CCAGTTTGTG GAGCACTGGA GGGAGCTGAA GGATCTGGCT
    GCTGCACGTG GGCAGAGGCT TGAAGAGTCG CTGGAATACC AGCAGTTTGT GGCGAACGTG
    GAAGAAGAGG AAGCCTGGAT CAACGAGAAA CTGAACCTGG TCGGAAGTGA AGATTACGGT
    GATACTCTGG CAGCTGTGCA GGGCTTGCTG AAGAAACATG AAGCATTTGA AACTGATTTT
    ACTGTGCACA GGGACCGTGT GAATGATGTG TGTTCCAATG GAGATGAACT CGTTAAAAAG
    GAGAACCACA ATGTGGAGAA CATCATGGCT AAAATGAAGG CGTTGCGTGG AAAAGTGTTG
    GAGCTGGAGA GAGCAGCGGC ACAGAGGAAA GCCAAGCTGG ATGAAAACTC TGCCTTCCTG
    CAGTTCAACT GGAAGGCTGA TGTGGTGGAG TCCTGGATTG GTGAGAAGGA AAACAGCCTG
    AAGACTGATG ACTATGGTCG CGATCTCTCC TCTGTGCAGA CGCTGCTCAC CAAACAGGAA
    ACCTTTGATG CAGGACTTCA GGCCTTCCAG CAGGAGGGCA TCACCAACAT CACAGCTCTA
    AAAGACCAGC TGCTGGCAGC CAAACATGTG CAGTCTAAGG CCATCGAGGC TCGTCACGCC
    ACCCTGATGA AGCGCTGGAA CCAACTACTA TCCAACTCAG CAGCCCGCAA GAAGAAGCTT
    CTGGAAGCAC AGGACCACTT CAGAAAGGTG GAGGATCTTT TCCTCACGTT TGCCAAGAAA
    GCCTCAGCCT TCAACAGCTG GTTTGAGAAC GCAGAGGAAG ATCTGACCGA TCCTGTGAGG
    TGCAACTCTC TGGAGGAGAT TAGGGCACTG CGGGATGCCC ATGATGCCTT CCGCTCGTCC
    CTGAGCTCCG CAGAGGCCGA CTTCAGCCAG CTGGCAGAAC TCGATCGGCA GATTAAGAGC
    TACAACGTGG TGTCCAACCC ATACACCTGG TTCACCATGG AGGCTCTGGA GGAGACCTGG
    AGTAACCTTC AGAAGATCAT CAAGGAGCGA GAACTGGAGC TGCAGAAGGA GCAGCGCCGA
    CAGGAAGAGA ATGATAAACT CAGACAGGAG TTTGCTCAGC ATGCTAATGC TTTCCACCAA
    TGGCTGCAGG AGACCAGGAC ATACCTTCTG GATGGCATAG CCTATCGGAG GGTAATCCGT
    GTCTATCAGT ATGAAGTCGA TGATGATCTT TCTGGAAGGT CCTGCATGGT GGAAGAATCT
    GGAACTCTAG AGTCACAGCT AGAAGCCACT AAGCGTAAGC ATCAGGAGAT TCGTGCCATG
    CGCAGTCAGC TGAAGAAGAT TGAGGATCTG GGAGCTGCCA TGGAGGAGGC GCTGATTCTG
    GACAATAAAT ACACAGAACA CAGCACCGTG GGTCTGGCAC AACAGTGGGA CCAACTCGAC
    CAGCTGGGCA TGAGAATGCA ACATAACCTG GAACAACAGA TCCAAGCCAG AAACACTACA
    GGTGTAACAG AAGAGGCCCT GAAGGAGTTC AGCATGATGT TCAAGCACTT CGACAAAGAA
    AAATCTGGCC GTCTGAATCA CCAGGAGTTC AAGTCTTGTC TGCGGTCTCT GGGTTACGAC
    CTTCCCATGG TGGAAGAAGG AGAACCAGAC CCAGAGTTTG AGTCCATCCT GGATATAGTG
    GATCCAAACA GGGATGGAAA CGTGTCCTTG CAGGAGTACA TGGCTTTCAT GATCAGCCGA
    GAAACGGAGA ACGTCAAGTC AAGCGAGGAG ATCGAGAGCG CTTTCCGTGC TCTGAGCGCA
    GAAAACAAAC CCTACGTCAC CAAGGAAGAA CTCTACCAGA ACCTGACAAA GGAACAAGCC
    GACTACTGCA TTTCCCATAT GAAGCCCTAC CTAGACAGCA AGGGCCGCGA AATCCCATCA
    GCTTTCGACT TTGTGGAATT CACCCGCTCG CTCTTCGTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324507.1
    CDS4..7428
    Translation

    Target ORF information:

    RefSeq Version XM_021468832.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468832.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGG ATGAAGCAGA CTCCAAAGGT
    GCATCTCCAT GGAAGGAGCT GAACAACCGC TGGAGGTCTC TGCAGCAGCT GGCTGAAGAC
    CGAAGCAACA TGCTGGGCAG TGCTCACGAA GTTCAGAGGT TCCACAGAGA TGCAGATGAG
    ACCAAGGAGT GGATCGAAGA GAAGAACCAA GCCCTGAACA CTGATAACTA TGGCCATGAC
    CTCGCCAGCG TGCAGGCTCT GCAGCGCAAG CATGAAGGAT TTGAGAGAGA TCTAGCCGCT
    TTAGGAGACA AGGTGAATTC TCTTGGTGAG ACTGCAGAGC GTCTGATTCA ATCTCATCCT
    GAGGCTGTGG ATGACATCCA GGAGAAGTGC ACCGAGCTAA ACACAGCATG GAGCAGCCTG
    GTGGGTCGTG CAGACCAGCG CAAAGAAAAA CTGGGCAACT CCCATGACCT GCAGCGCTTC
    CTCAGTGACT TCAGAGATCT CATGTCTTGG ATCAATGGAA TCAGGGGACT TGTGTCTTCC
    GATGAGCTGG CCAAGGATGT TACTGGTGCT GAAGCCCTGT TGGAGAGACA TCAGGAACAC
    CGCACTGAGA TCGACGCTCG TGCAGGAACC TTCCTGGCTT TTGAGCAGTT TGGACAGCAG
    CTGCTCGCTC GTGGGCATTA CGCAAGCCCT GAGATCAAAC AGAAGCTGGA GGCTCTTGAC
    CGGGAGCGTG CTGACCTGGA GAAGGCCTGG GTTCAGCGCA GGATGATGCT GGACCAGTGC
    CTGGAGCTGC AGTTGTTTAA CCGTGACTGT GAGCAGGCTG AGAACTGGAT GGCTGCCCGC
    GAAGCCTTCT TAGCCAGCGA TGACAAGGGA GATTCTCTGG ACAGTGTGGA GGCGCTCATT
    AAGAAACACG AGGACTTTGA TAAGGCTATC AATGTGCAGG AAGAAAAGAT TGCTGCACTG
    CAGTCTTTTG CTGACCAGCT CATCAGCGCT GACCACTATG CCAAGCCTGA GATCTTCAAA
    CGACGCAGTG AGGTCTTGGA CAGGTGGCTC CGCCTCAAGG CTCAGATGAT TGAGAAACGC
    TCCAAGCTTG GCGAGTCACA GACTCTACAG CAGTTCAGCA GAGACGTTGA CGAGATCGAA
    GCTTGGATCA GTGAGAAACT CCAGACCGCC ACAGACGAGT CTTACAAAGA CCCCACCAAC
    ATCCAGCTGT CCAAGCTGCT GAGCAAACAC CAGAAACACC AGGCTTTTGA GGCTGAGCTA
    CACGCTAACG CTGATCGTAT CCGCGGAGTG ATTGACACGG GCAATGCTCT CATCCAGAGA
    GGTGCCTGTG CTGGAAGTGA AGATGCTGTT AAGGCTCGTC TGAATGCTCT GGACGAGCAG
    TGGCAGTTCC TGGTCAACAA GTCTGCTGAG AAGAGTCAGA AACTGAAGGA GGCCAACAAA
    CAGCAGAACT TCAACACTGG CATCAAGGAC TTTGACTTCT GGTTGTCTGA GGTTGAGGCT
    CTTCTTGCCT CTGAGGATTA TGGGAAAGAT CTGGCTTCAG TCAACAATCT GCTGAAGAAG
    CACCAGCTGT TGGAGGCTGA TATCTCTGCT CATGAGGATC GTTTGAAGGA TCTTAATGGC
    CAGGCTGACA GTCTGATGGC CAGCAACGCT TTCGACACAT CTCAGGTCAA AGACAAGCGG
    GATGCCGTTA ACGGACGCTT CGGCAAAATC AAGAGCATGG CAGCGGGACG GCGAGCCAAG
    CTGAACGAAT CGCATCGTCT GCACCAGTTC TTCAGGGATC TGGACGATGA GGAGTCCTGG
    ATTAAGGAAA AGAAGTTGTT GGTGAGCTCG GAGGACTACG GACGTGATTT GACAGGGGTG
    CAGAATCTGA GGAAGAAGCA CAAGAGGCTG GAGGCTGAGC TGGGAGCACA CGAGCCGGCC
    ATTCAGTCTG TGTTGGAGAC TGGGAAGAAG CTGTCTGACG ACAACACCAT TGGACAGGAG
    GAGATCCAGC AGAGACTGGC CCAGTTTGTG GAGCACTGGA GGGAGCTGAA GGATCTGGCT
    GCTGCACGTG GGCAGAGGCT TGAAGAGTCG CTGGAATACC AGCAGTTTGT GGCGAACGTG
    GAAGAAGAGG AAGCCTGGAT CAACGAGAAA CTGAACCTGG TCGGAAGTGA AGATTACGGT
    GATACTCTGG CAGCTGTGCA GGGCTTGCTG AAGAAACATG AAGCATTTGA AACTGATTTT
    ACTGTGCACA GGGACCGTGT GAATGATGTG TGTTCCAATG GAGATGAACT CGTTAAAAAG
    GAGAACCACA ATGTGGAGAA CATCATGGCT AAAATGAAGG CGTTGCGTGG AAAAGTGTTG
    GAGCTGGAGA GAGCAGCGGC ACAGAGGAAA GCCAAGCTGG ATGAAAACTC TGCCTTCCTG
    CAGTTCAACT GGAAGGCTGA TGTGGTGGAG TCCTGGATTG GTGAGAAGGA AAACAGCCTG
    AAGACTGATG ACTATGGTCG CGATCTCTCC TCTGTGCAGA CGCTGCTCAC CAAACAGGAA
    ACCTTTGATG CAGGACTTCA GGCCTTCCAG CAGGAGGGCA TCACCAACAT CACAGCTCTA
    AAAGACCAGC TGCTGGCAGC CAAACATGTG CAGTCTAAGG CCATCGAGGC TCGTCACGCC
    ACCCTGATGA AGCGCTGGAA CCAACTACTA TCCAACTCAG CAGCCCGCAA GAAGAAGCTT
    CTGGAAGCAC AGGACCACTT CAGAAAGGTG GAGGATCTTT TCCTCACGTT TGCCAAGAAA
    GCCTCAGCCT TCAACAGCTG GTTTGAGAAC GCAGAGGAAG ATCTGACCGA TCCTGTGAGG
    TGCAACTCTC TGGAGGAGAT TAGGGCACTG CGGGATGCCC ATGATGCCTT CCGCTCGTCC
    CTGAGCTCCG CAGAGGCCGA CTTCAGCCAG CTGGCAGAAC TCGATCGGCA GATTAAGAGC
    TACAACGTGG TGTCCAACCC ATACACCTGG TTCACCATGG AGGCTCTGGA GGAGACCTGG
    AGTAACCTTC AGAAGATCAT CAAGGAGCGA GAACTGGAGC TGCAGAAGGA GCAGCGCCGA
    CAGGAAGAGA ATGATAAACT CAGACAGGAG TTTGCTCAGC ATGCTAATGC TTTCCACCAA
    TGGCTGCAGG AGACCAGGAC ATACCTTCTG GATGGCATAG CCTATCGGAG GGTAATCCGT
    GTCTATCAGT ATGAAGTCGA TGATGATCTT TCTGGAAGGT CCTGCATGGT GGAAGAATCT
    GGAACTCTAG AGTCACAGCT AGAAGCCACT AAGCGTAAGC ATCAGGAGAT TCGTGCCATG
    CGCAGTCAGC TGAAGAAGAT TGAGGATCTG GGAGCTGCCA TGGAGGAGGC GCTGATTCTG
    GACAATAAAT ACACAGAACA CAGCACCGTG GGTCTGGCAC AACAGTGGGA CCAACTCGAC
    CAGCTGGGCA TGAGAATGCA ACATAACCTG GAACAACAGA TCCAAGCCAG AAACACTACA
    GGTGTAACAG AAGAGGCCCT GAAGGAGTTC AGCATGATGT TCAAGCACTT CGACAAAGAA
    AAATCTGGCC GTCTGAATCA CCAGGAGTTC AAGTCTTGTC TGCGGTCTCT GGGTTACGAC
    CTTCCCATGG TGGAAGAAGG AGAACCAGAC CCAGAGTTTG AGTCCATCCT GGATATAGTG
    GATCCAAACA GGGATGGAAA CGTGTCCTTG CAGGAGTACA TGGCTTTCAT GATCAGCCGA
    GAAACGGAGA ACGTCAAGTC AAGCGAGGAG ATCGAGAGCG CTTTCCGTGC TCTGAGCGCA
    GAAAACAAAC CCTACGTCAC CAAGGAAGAA CTCTACCAGA ACCTGACAAA GGAACAAGCC
    GACTACTGCA TTTCCCATAT GAAGCCCTAC CTAGACAGCA AGGGCCGCGA AATCCCATCA
    GCTTTCGACT TTGTGGAATT CACCCGCTCG CTCTTCGTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa33721
    Clone ID Related Accession (Same CDS sequence) NM_001098488.1
    Accession Version NM_001098488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF375552.1. On Jun 6, 2007 this sequence version replaced XR_028969.1. ##Evidence-Data-START## Transcript exon combination :: EF375552.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATA
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCGGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAATT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGG
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGGAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAATTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCCGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCGGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAG TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGAC TGGAAGAGTC GCTGGAGTAC
    CAGCAGTTTG TGGCGAACGT GGAGGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAC
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC AAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTT
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACTCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCTTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACTG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TCAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGGTCC
    TGCATGGTGG AAGAATCTGG AACTCTAGAG TCACAGCTAG AAGCCACTAA GCGTAAGCAT
    CAGGAGATTC GTGCCATGCG CAGTCAGCTG AAGAAGATTG AGGATCTGGG AGCTGCCATG
    GAGGAGGCGC TGATTCTGGA CAATAAATAC ACAGAACACA GCACCGTGGG TCTGGCACAA
    CAGTGGGACC AACTCGACCA GCTGGGCATG AGAATGCAAC ATAACCTGGA ACAACAGATC
    CAAGCCAGAA ACACTACAGG TGTAACAGAA GAGGCCCTGA AGGAGTTCAG CATGATGTTC
    AAGCACTTCG ACAAAGAAAA ATCTGGCCGT CTGAATCACC AGGAGTTCAA GTCTTGTCTG
    CGGTCTCTGG GTTACGACCT TCCCATGGTG GAAGAAGGAG AACCAGACCC AGAGTTTGAG
    TCCATCCTGG ATATAGTGGA TCCAAACAGG GATGGAAACG TGTCCTTGCA GGAGTACATG
    GCTTTCATGA TCAGCCGAGA AACAGAGAAC GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT
    TTCCGTGCTC TGAGCGCAGA AAACAAACCC TACGTCACCA AGGAAGAACT CTACCAGAAC
    CTGACAAAGG AACAAGCCGA CTACTGCATT TCCCATATGA AGCCCTACCT AGACAGCAAG
    GGCCGCGAAA TCCCATCAGC TTTCGACTTT GTGGAATTCA CCCGCTCGCT CTTCGTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091958.1
    CDS84..7526
    Translation

    Target ORF information:

    RefSeq Version NM_001098488.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha, non-erythrocytic 1 (sptan1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATA
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCGGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAATT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGG
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGGAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAATTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCCGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCGGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAG TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGAC TGGAAGAGTC GCTGGAGTAC
    CAGCAGTTTG TGGCGAACGT GGAGGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAC
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC AAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTT
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACTCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCTTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACTG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TCAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGGTCC
    TGCATGGTGG AAGAATCTGG AACTCTAGAG TCACAGCTAG AAGCCACTAA GCGTAAGCAT
    CAGGAGATTC GTGCCATGCG CAGTCAGCTG AAGAAGATTG AGGATCTGGG AGCTGCCATG
    GAGGAGGCGC TGATTCTGGA CAATAAATAC ACAGAACACA GCACCGTGGG TCTGGCACAA
    CAGTGGGACC AACTCGACCA GCTGGGCATG AGAATGCAAC ATAACCTGGA ACAACAGATC
    CAAGCCAGAA ACACTACAGG TGTAACAGAA GAGGCCCTGA AGGAGTTCAG CATGATGTTC
    AAGCACTTCG ACAAAGAAAA ATCTGGCCGT CTGAATCACC AGGAGTTCAA GTCTTGTCTG
    CGGTCTCTGG GTTACGACCT TCCCATGGTG GAAGAAGGAG AACCAGACCC AGAGTTTGAG
    TCCATCCTGG ATATAGTGGA TCCAAACAGG GATGGAAACG TGTCCTTGCA GGAGTACATG
    GCTTTCATGA TCAGCCGAGA AACAGAGAAC GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT
    TTCCGTGCTC TGAGCGCAGA AAACAAACCC TACGTCACCA AGGAAGAACT CTACCAGAAC
    CTGACAAAGG AACAAGCCGA CTACTGCATT TCCCATATGA AGCCCTACCT AGACAGCAAG
    GGCCGCGAAA TCCCATCAGC TTTCGACTTT GTGGAATTCA CCCGCTCGCT CTTCGTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41932
    Clone ID Related Accession (Same CDS sequence) XM_009295198.2
    Accession Version XM_009295198.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGGTCC
    TGCATGGTGG AAGAATCTGG AACTCTAGAG TCACAGCTAG AAGCCACTAA GCGTAAGCAT
    CAGGAGATTC GTGCCATGCG CAGTCAGCTG AAGAAGATTG AGGATCTGGG AGCTGCCATG
    GAGGAGGCGC TGATTCTGGA CAATAAATAC ACAGAACACA GCACCGTGGG TCTGGCACAA
    CAGTGGGACC AACTCGACCA GCTGGGCATG AGAATGCAAC ATAACCTGGA ACAACAGATC
    CAAGCCAGAA ACACTACAGG TGTAACAGAA GAGGCCCTGA AGGAGTTCAG CATGATGTTC
    AAGCACTTCG ACAAAGAAAA ATCTGGCCGT CTGAATCACC AGGAGTTCAA GTCTTGTCTG
    CGGTCTCTGG GTTACGACCT TCCCATGGTG GAAGAAGGAG AACCAGACCC AGAGTTTGAG
    TCCATCCTGG ATATAGTGGA TCCAAACAGG GATGGAAACG TGTCCTTGCA GGAGTACATG
    GCTTTCATGA TCAGCCGAGA AACGGAGAAC GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT
    TTCCGTGCTC TGAGCGCAGA AAACAAACCC TACGTCACCA AGGAAGAACT CTACCAGAAC
    CTGACAAAGG AACAAGCCGA CTACTGCATT TCCCATATGA AGCCCTACCT AGACAGCAAG
    GGCCGCGAAA TCCCATCAGC TTTCGACTTT GTGGAATTCA CCCGCTCGCT CTTCGTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293473.1
    CDS222..7664
    Translation

    Target ORF information:

    RefSeq Version XM_009295198.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295198.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGGTCC
    TGCATGGTGG AAGAATCTGG AACTCTAGAG TCACAGCTAG AAGCCACTAA GCGTAAGCAT
    CAGGAGATTC GTGCCATGCG CAGTCAGCTG AAGAAGATTG AGGATCTGGG AGCTGCCATG
    GAGGAGGCGC TGATTCTGGA CAATAAATAC ACAGAACACA GCACCGTGGG TCTGGCACAA
    CAGTGGGACC AACTCGACCA GCTGGGCATG AGAATGCAAC ATAACCTGGA ACAACAGATC
    CAAGCCAGAA ACACTACAGG TGTAACAGAA GAGGCCCTGA AGGAGTTCAG CATGATGTTC
    AAGCACTTCG ACAAAGAAAA ATCTGGCCGT CTGAATCACC AGGAGTTCAA GTCTTGTCTG
    CGGTCTCTGG GTTACGACCT TCCCATGGTG GAAGAAGGAG AACCAGACCC AGAGTTTGAG
    TCCATCCTGG ATATAGTGGA TCCAAACAGG GATGGAAACG TGTCCTTGCA GGAGTACATG
    GCTTTCATGA TCAGCCGAGA AACGGAGAAC GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT
    TTCCGTGCTC TGAGCGCAGA AAACAAACCC TACGTCACCA AGGAAGAACT CTACCAGAAC
    CTGACAAAGG AACAAGCCGA CTACTGCATT TCCCATATGA AGCCCTACCT AGACAGCAAG
    GGCCGCGAAA TCCCATCAGC TTTCGACTTT GTGGAATTCA CCCGCTCGCT CTTCGTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41933
    Clone ID Related Accession (Same CDS sequence) XM_021468830.1 , XM_009295199.2
    Accession Version XM_021468830.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GGTCCTGCAT GGTGGAAGAA
    TCTGGAACTC TAGAGTCACA GCTAGAAGCC ACTAAGCGTA AGCATCAGGA GATTCGTGCC
    ATGCGCAGTC AGCTGAAGAA GATTGAGGAT CTGGGAGCTG CCATGGAGGA GGCGCTGATT
    CTGGACAATA AATACACAGA ACACAGCACC GTGGGTCTGG CACAACAGTG GGACCAACTC
    GACCAGCTGG GCATGAGAAT GCAACATAAC CTGGAACAAC AGATCCAAGC CAGAAACACT
    ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG TTCAGCATGA TGTTCAAGCA CTTCGACAAA
    GAAAAATCTG GCCGTCTGAA TCACCAGGAG TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC
    GACCTTCCCA TGGTGGAAGA AGGAGAACCA GACCCAGAGT TTGAGTCCAT CCTGGATATA
    GTGGATCCAA ACAGGGATGG AAACGTGTCC TTGCAGGAGT ACATGGCTTT CATGATCAGC
    CGAGAAACGG AGAACGTCAA GTCAAGCGAG GAGATCGAGA GCGCTTTCCG TGCTCTGAGC
    GCAGAAAACA AACCCTACGT CACCAAGGAA GAACTCTACC AGAACCTGAC AAAGGAACAA
    GCCGACTACT GCATTTCCCA TATGAAGCCC TACCTAGACA GCAAGGGCCG CGAAATCCCA
    TCAGCTTTCG ACTTTGTGGA ATTCACCCGC TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324505.1
    CDS4..7431
    Translation

    Target ORF information:

    RefSeq Version XM_021468830.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468830.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GGTCCTGCAT GGTGGAAGAA
    TCTGGAACTC TAGAGTCACA GCTAGAAGCC ACTAAGCGTA AGCATCAGGA GATTCGTGCC
    ATGCGCAGTC AGCTGAAGAA GATTGAGGAT CTGGGAGCTG CCATGGAGGA GGCGCTGATT
    CTGGACAATA AATACACAGA ACACAGCACC GTGGGTCTGG CACAACAGTG GGACCAACTC
    GACCAGCTGG GCATGAGAAT GCAACATAAC CTGGAACAAC AGATCCAAGC CAGAAACACT
    ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG TTCAGCATGA TGTTCAAGCA CTTCGACAAA
    GAAAAATCTG GCCGTCTGAA TCACCAGGAG TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC
    GACCTTCCCA TGGTGGAAGA AGGAGAACCA GACCCAGAGT TTGAGTCCAT CCTGGATATA
    GTGGATCCAA ACAGGGATGG AAACGTGTCC TTGCAGGAGT ACATGGCTTT CATGATCAGC
    CGAGAAACGG AGAACGTCAA GTCAAGCGAG GAGATCGAGA GCGCTTTCCG TGCTCTGAGC
    GCAGAAAACA AACCCTACGT CACCAAGGAA GAACTCTACC AGAACCTGAC AAAGGAACAA
    GCCGACTACT GCATTTCCCA TATGAAGCCC TACCTAGACA GCAAGGGCCG CGAAATCCCA
    TCAGCTTTCG ACTTTGTGGA ATTCACCCGC TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41933
    Clone ID Related Accession (Same CDS sequence) XM_021468830.1 , XM_009295199.2
    Accession Version XM_009295199.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    601
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    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GGTCCTGCAT GGTGGAAGAA
    TCTGGAACTC TAGAGTCACA GCTAGAAGCC ACTAAGCGTA AGCATCAGGA GATTCGTGCC
    ATGCGCAGTC AGCTGAAGAA GATTGAGGAT CTGGGAGCTG CCATGGAGGA GGCGCTGATT
    CTGGACAATA AATACACAGA ACACAGCACC GTGGGTCTGG CACAACAGTG GGACCAACTC
    GACCAGCTGG GCATGAGAAT GCAACATAAC CTGGAACAAC AGATCCAAGC CAGAAACACT
    ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG TTCAGCATGA TGTTCAAGCA CTTCGACAAA
    GAAAAATCTG GCCGTCTGAA TCACCAGGAG TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC
    GACCTTCCCA TGGTGGAAGA AGGAGAACCA GACCCAGAGT TTGAGTCCAT CCTGGATATA
    GTGGATCCAA ACAGGGATGG AAACGTGTCC TTGCAGGAGT ACATGGCTTT CATGATCAGC
    CGAGAAACGG AGAACGTCAA GTCAAGCGAG GAGATCGAGA GCGCTTTCCG TGCTCTGAGC
    GCAGAAAACA AACCCTACGT CACCAAGGAA GAACTCTACC AGAACCTGAC AAAGGAACAA
    GCCGACTACT GCATTTCCCA TATGAAGCCC TACCTAGACA GCAAGGGCCG CGAAATCCCA
    TCAGCTTTCG ACTTTGTGGA ATTCACCCGC TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293474.1
    CDS114..7541
    Translation

    Target ORF information:

    RefSeq Version XM_009295199.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295199.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GGTCCTGCAT GGTGGAAGAA
    TCTGGAACTC TAGAGTCACA GCTAGAAGCC ACTAAGCGTA AGCATCAGGA GATTCGTGCC
    ATGCGCAGTC AGCTGAAGAA GATTGAGGAT CTGGGAGCTG CCATGGAGGA GGCGCTGATT
    CTGGACAATA AATACACAGA ACACAGCACC GTGGGTCTGG CACAACAGTG GGACCAACTC
    GACCAGCTGG GCATGAGAAT GCAACATAAC CTGGAACAAC AGATCCAAGC CAGAAACACT
    ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG TTCAGCATGA TGTTCAAGCA CTTCGACAAA
    GAAAAATCTG GCCGTCTGAA TCACCAGGAG TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC
    GACCTTCCCA TGGTGGAAGA AGGAGAACCA GACCCAGAGT TTGAGTCCAT CCTGGATATA
    GTGGATCCAA ACAGGGATGG AAACGTGTCC TTGCAGGAGT ACATGGCTTT CATGATCAGC
    CGAGAAACGG AGAACGTCAA GTCAAGCGAG GAGATCGAGA GCGCTTTCCG TGCTCTGAGC
    GCAGAAAACA AACCCTACGT CACCAAGGAA GAACTCTACC AGAACCTGAC AAAGGAACAA
    GCCGACTACT GCATTTCCCA TATGAAGCCC TACCTAGACA GCAAGGGCCG CGAAATCCCA
    TCAGCTTTCG ACTTTGTGGA ATTCACCCGC TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41934
    Clone ID Related Accession (Same CDS sequence) XM_021468831.1 , XM_009295200.2
    Accession Version XM_021468831.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGT CCTGCATGGT GGAAGAATCT
    GGAACTCTAG AGTCACAGCT AGAAGCCACT AAGCGTAAGC ATCAGGAGAT TCGTGCCATG
    CGCAGTCAGC TGAAGAAGAT TGAGGATCTG GGAGCTGCCA TGGAGGAGGC GCTGATTCTG
    GACAATAAAT ACACAGAACA CAGCACCGTG GGTCTGGCAC AACAGTGGGA CCAACTCGAC
    CAGCTGGGCA TGAGAATGCA ACATAACCTG GAACAACAGA TCCAAGCCAG AAACACTACA
    GGTGTAACAG AAGAGGCCCT GAAGGAGTTC AGCATGATGT TCAAGCACTT CGACAAAGAA
    AAATCTGGCC GTCTGAATCA CCAGGAGTTC AAGTCTTGTC TGCGGTCTCT GGGTTACGAC
    CTTCCCATGG TGGAAGAAGG AGAACCAGAC CCAGAGTTTG AGTCCATCCT GGATATAGTG
    GATCCAAACA GGGATGGAAA CGTGTCCTTG CAGGAGTACA TGGCTTTCAT GATCAGCCGA
    GAAACGGAGA ACGTCAAGTC AAGCGAGGAG ATCGAGAGCG CTTTCCGTGC TCTGAGCGCA
    GAAAACAAAC CCTACGTCAC CAAGGAAGAA CTCTACCAGA ACCTGACAAA GGAACAAGCC
    GACTACTGCA TTTCCCATAT GAAGCCCTAC CTAGACAGCA AGGGCCGCGA AATCCCATCA
    GCTTTCGACT TTGTGGAATT CACCCGCTCG CTCTTCGTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324506.1
    CDS4..7428
    Translation

    Target ORF information:

    RefSeq Version XM_021468831.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468831.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGT CCTGCATGGT GGAAGAATCT
    GGAACTCTAG AGTCACAGCT AGAAGCCACT AAGCGTAAGC ATCAGGAGAT TCGTGCCATG
    CGCAGTCAGC TGAAGAAGAT TGAGGATCTG GGAGCTGCCA TGGAGGAGGC GCTGATTCTG
    GACAATAAAT ACACAGAACA CAGCACCGTG GGTCTGGCAC AACAGTGGGA CCAACTCGAC
    CAGCTGGGCA TGAGAATGCA ACATAACCTG GAACAACAGA TCCAAGCCAG AAACACTACA
    GGTGTAACAG AAGAGGCCCT GAAGGAGTTC AGCATGATGT TCAAGCACTT CGACAAAGAA
    AAATCTGGCC GTCTGAATCA CCAGGAGTTC AAGTCTTGTC TGCGGTCTCT GGGTTACGAC
    CTTCCCATGG TGGAAGAAGG AGAACCAGAC CCAGAGTTTG AGTCCATCCT GGATATAGTG
    GATCCAAACA GGGATGGAAA CGTGTCCTTG CAGGAGTACA TGGCTTTCAT GATCAGCCGA
    GAAACGGAGA ACGTCAAGTC AAGCGAGGAG ATCGAGAGCG CTTTCCGTGC TCTGAGCGCA
    GAAAACAAAC CCTACGTCAC CAAGGAAGAA CTCTACCAGA ACCTGACAAA GGAACAAGCC
    GACTACTGCA TTTCCCATAT GAAGCCCTAC CTAGACAGCA AGGGCCGCGA AATCCCATCA
    GCTTTCGACT TTGTGGAATT CACCCGCTCG CTCTTCGTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41934
    Clone ID Related Accession (Same CDS sequence) XM_021468831.1 , XM_009295200.2
    Accession Version XM_009295200.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGT CCTGCATGGT GGAAGAATCT
    GGAACTCTAG AGTCACAGCT AGAAGCCACT AAGCGTAAGC ATCAGGAGAT TCGTGCCATG
    CGCAGTCAGC TGAAGAAGAT TGAGGATCTG GGAGCTGCCA TGGAGGAGGC GCTGATTCTG
    GACAATAAAT ACACAGAACA CAGCACCGTG GGTCTGGCAC AACAGTGGGA CCAACTCGAC
    CAGCTGGGCA TGAGAATGCA ACATAACCTG GAACAACAGA TCCAAGCCAG AAACACTACA
    GGTGTAACAG AAGAGGCCCT GAAGGAGTTC AGCATGATGT TCAAGCACTT CGACAAAGAA
    AAATCTGGCC GTCTGAATCA CCAGGAGTTC AAGTCTTGTC TGCGGTCTCT GGGTTACGAC
    CTTCCCATGG TGGAAGAAGG AGAACCAGAC CCAGAGTTTG AGTCCATCCT GGATATAGTG
    GATCCAAACA GGGATGGAAA CGTGTCCTTG CAGGAGTACA TGGCTTTCAT GATCAGCCGA
    GAAACGGAGA ACGTCAAGTC AAGCGAGGAG ATCGAGAGCG CTTTCCGTGC TCTGAGCGCA
    GAAAACAAAC CCTACGTCAC CAAGGAAGAA CTCTACCAGA ACCTGACAAA GGAACAAGCC
    GACTACTGCA TTTCCCATAT GAAGCCCTAC CTAGACAGCA AGGGCCGCGA AATCCCATCA
    GCTTTCGACT TTGTGGAATT CACCCGCTCG CTCTTCGTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293475.1
    CDS114..7538
    Translation

    Target ORF information:

    RefSeq Version XM_009295200.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGT CCTGCATGGT GGAAGAATCT
    GGAACTCTAG AGTCACAGCT AGAAGCCACT AAGCGTAAGC ATCAGGAGAT TCGTGCCATG
    CGCAGTCAGC TGAAGAAGAT TGAGGATCTG GGAGCTGCCA TGGAGGAGGC GCTGATTCTG
    GACAATAAAT ACACAGAACA CAGCACCGTG GGTCTGGCAC AACAGTGGGA CCAACTCGAC
    CAGCTGGGCA TGAGAATGCA ACATAACCTG GAACAACAGA TCCAAGCCAG AAACACTACA
    GGTGTAACAG AAGAGGCCCT GAAGGAGTTC AGCATGATGT TCAAGCACTT CGACAAAGAA
    AAATCTGGCC GTCTGAATCA CCAGGAGTTC AAGTCTTGTC TGCGGTCTCT GGGTTACGAC
    CTTCCCATGG TGGAAGAAGG AGAACCAGAC CCAGAGTTTG AGTCCATCCT GGATATAGTG
    GATCCAAACA GGGATGGAAA CGTGTCCTTG CAGGAGTACA TGGCTTTCAT GATCAGCCGA
    GAAACGGAGA ACGTCAAGTC AAGCGAGGAG ATCGAGAGCG CTTTCCGTGC TCTGAGCGCA
    GAAAACAAAC CCTACGTCAC CAAGGAAGAA CTCTACCAGA ACCTGACAAA GGAACAAGCC
    GACTACTGCA TTTCCCATAT GAAGCCCTAC CTAGACAGCA AGGGCCGCGA AATCCCATCA
    GCTTTCGACT TTGTGGAATT CACCCGCTCG CTCTTCGTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41935
    Clone ID Related Accession (Same CDS sequence) XM_009295201.2
    Accession Version XM_009295201.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGG ATGAAGCAGA CTCCAAAGGT
    GCATCTCCAT GGAAGTCTGT TCGCTTGGCT GTTCAAACAA CGGCCAACTT TAATACTATC
    AAGGAGCTGA ACAACCGCTG GAGGTCTCTG CAGCAGCTGG CTGAAGACCG AAGCAACATG
    CTGGGCAGTG CTCACGAAGT TCAGAGGTTC CACAGAGATG CAGATGAGAC CAAGGAGTGG
    ATCGAAGAGA AGAACCAAGC CCTGAACACT GATAACTATG GCCATGACCT CGCCAGCGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGATTT GAGAGAGATC TAGCCGCTTT AGGAGACAAG
    GTGAATTCTC TTGGTGAGAC TGCAGAGCGT CTGATTCAAT CTCATCCTGA GGCTGTGGAT
    GACATCCAGG AGAAGTGCAC CGAGCTAAAC ACAGCATGGA GCAGCCTGGT GGGTCGTGCA
    GACCAGCGCA AAGAAAAACT GGGCAACTCC CATGACCTGC AGCGCTTCCT CAGTGACTTC
    AGAGATCTCA TGTCTTGGAT CAATGGAATC AGGGGACTTG TGTCTTCCGA TGAGCTGGCC
    AAGGATGTTA CTGGTGCTGA AGCCCTGTTG GAGAGACATC AGGAACACCG CACTGAGATC
    GACGCTCGTG CAGGAACCTT CCTGGCTTTT GAGCAGTTTG GACAGCAGCT GCTCGCTCGT
    GGGCATTACG CAAGCCCTGA GATCAAACAG AAGCTGGAGG CTCTTGACCG GGAGCGTGCT
    GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ACCAGTGCCT GGAGCTGCAG
    TTGTTTAACC GTGACTGTGA GCAGGCTGAG AACTGGATGG CTGCCCGCGA AGCCTTCTTA
    GCCAGCGATG ACAAGGGAGA TTCTCTGGAC AGTGTGGAGG CGCTCATTAA GAAACACGAG
    GACTTTGATA AGGCTATCAA TGTGCAGGAA GAAAAGATTG CTGCACTGCA GTCTTTTGCT
    GACCAGCTCA TCAGCGCTGA CCACTATGCC AAGCCTGAGA TCTTCAAACG ACGCAGTGAG
    GTCTTGGACA GGTGGCTCCG CCTCAAGGCT CAGATGATTG AGAAACGCTC CAAGCTTGGC
    GAGTCACAGA CTCTACAGCA GTTCAGCAGA GACGTTGACG AGATCGAAGC TTGGATCAGT
    GAGAAACTCC AGACCGCCAC AGACGAGTCT TACAAAGACC CCACCAACAT CCAGCTGTCC
    AAGCTGCTGA GCAAACACCA GAAACACCAG GCTTTTGAGG CTGAGCTACA CGCTAACGCT
    GATCGTATCC GCGGAGTGAT TGACACGGGC AATGCTCTCA TCCAGAGAGG TGCCTGTGCT
    GGAAGTGAAG ATGCTGTTAA GGCTCGTCTG AATGCTCTGG ACGAGCAGTG GCAGTTCCTG
    GTCAACAAGT CTGCTGAGAA GAGTCAGAAA CTGAAGGAGG CCAACAAACA GCAGAACTTC
    AACACTGGCA TCAAGGACTT TGACTTCTGG TTGTCTGAGG TTGAGGCTCT TCTTGCCTCT
    GAGGATTATG GGAAAGATCT GGCTTCAGTC AACAATCTGC TGAAGAAGCA CCAGCTGTTG
    GAGGCTGATA TCTCTGCTCA TGAGGATCGT TTGAAGGATC TTAATGGCCA GGCTGACAGT
    CTGATGGCCA GCAACGCTTT CGACACATCT CAGGTCAAAG ACAAGCGGGA TGCCGTTAAC
    GGACGCTTCG GCAAAATCAA GAGCATGGCA GCGGGACGGC GAGCCAAGCT GAACGAATCG
    CATCGTCTGC ACCAGTTCTT CAGGGATCTG GACGATGAGG AGTCCTGGAT TAAGGAAAAG
    AAGTTGTTGG TGAGCTCGGA GGACTACGGA CGTGATTTGA CAGGGGTGCA GAATCTGAGG
    AAGAAGCACA AGAGGCTGGA GGCTGAGCTG GGAGCACACG AGCCGGCCAT TCAGTCTGTG
    TTGGAGACTG GGAAGAAGCT GTCTGACGAC AACACCATTG GACAGGAGGA GATCCAGCAG
    AGACTGGCCC AGTTTGTGGA GCACTGGAGG GAGCTGAAGG ATCTGGCTGC TGCACGTGGG
    CAGAGGCTTG AAGAGTCGCT GGAATACCAG CAGTTTGTGG CGAACGTGGA AGAAGAGGAA
    GCCTGGATCA ACGAGAAACT GAACCTGGTC GGAAGTGAAG ATTACGGTGA TACTCTGGCA
    GCTGTGCAGG GCTTGCTGAA GAAACATGAA GCATTTGAAA CTGATTTTAC TGTGCACAGG
    GACCGTGTGA ATGATGTGTG TTCCAATGGA GATGAACTCG TTAAAAAGGA GAACCACAAT
    GTGGAGAACA TCATGGCTAA AATGAAGGCG TTGCGTGGAA AAGTGTTGGA GCTGGAGAGA
    GCAGCGGCAC AGAGGAAAGC CAAGCTGGAT GAAAACTCTG CCTTCCTGCA GTTCAACTGG
    AAGGCTGATG TGGTGGAGTC CTGGATTGGT GAGAAGGAAA ACAGCCTGAA GACTGATGAC
    TATGGTCGCG ATCTCTCCTC TGTGCAGACG CTGCTCACCA AACAGGAAAC CTTTGATGCA
    GGACTTCAGG CCTTCCAGCA GGAGGGCATC ACCAACATCA CAGCTCTAAA AGACCAGCTG
    CTGGCAGCCA AACATGTGCA GTCTAAGGCC ATCGAGGCTC GTCACGCCAC CCTGATGAAG
    CGCTGGAACC AACTACTATC CAACTCAGCA GCCCGCAAGA AGAAGCTTCT GGAAGCACAG
    GACCACTTCA GAAAGGTGGA GGATCTTTTC CTCACGTTTG CCAAGAAAGC CTCAGCCTTC
    AACAGCTGGT TTGAGAACGC AGAGGAAGAT CTGACCGATC CTGTGAGGTG CAACTCTCTG
    GAGGAGATTA GGGCACTGCG GGATGCCCAT GATGCCTTCC GCTCGTCCCT GAGCTCCGCA
    GAGGCCGACT TCAGCCAGCT GGCAGAACTC GATCGGCAGA TTAAGAGCTA CAACGTGGTG
    TCCAACCCAT ACACCTGGTT CACCATGGAG GCTCTGGAGG AGACCTGGAG TAACCTTCAG
    AAGATCATCA AGGAGCGAGA ACTGGAGCTG CAGAAGGAGC AGCGCCGACA GGAAGAGAAT
    GATAAACTCA GACAGGAGTT TGCTCAGCAT GCTAATGCTT TCCACCAATG GCTGCAGGAG
    ACCAGGACAT ACCTTCTGGA TGGGTCCTGC ATGGTGGAAG AATCTGGAAC TCTAGAGTCA
    CAGCTAGAAG CCACTAAGCG TAAGCATCAG GAGATTCGTG CCATGCGCAG TCAGCTGAAG
    AAGATTGAGG ATCTGGGAGC TGCCATGGAG GAGGCGCTGA TTCTGGACAA TAAATACACA
    GAACACAGCA CCGTGGGTCT GGCACAACAG TGGGACCAAC TCGACCAGCT GGGCATGAGA
    ATGCAACATA ACCTGGAACA ACAGATCCAA GCCAGAAACA CTACAGGTGT AACAGAAGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTCGACA AAGAAAAATC TGGCCGTCTG
    AATCACCAGG AGTTCAAGTC TTGTCTGCGG TCTCTGGGTT ACGACCTTCC CATGGTGGAA
    GAAGGAGAAC CAGACCCAGA GTTTGAGTCC ATCCTGGATA TAGTGGATCC AAACAGGGAT
    GGAAACGTGT CCTTGCAGGA GTACATGGCT TTCATGATCA GCCGAGAAAC GGAGAACGTC
    AAGTCAAGCG AGGAGATCGA GAGCGCTTTC CGTGCTCTGA GCGCAGAAAA CAAACCCTAC
    GTCACCAAGG AAGAACTCTA CCAGAACCTG ACAAAGGAAC AAGCCGACTA CTGCATTTCC
    CATATGAAGC CCTACCTAGA CAGCAAGGGC CGCGAAATCC CATCAGCTTT CGACTTTGTG
    GAATTCACCC GCTCGCTCTT CGTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293476.1
    CDS114..7523
    Translation

    Target ORF information:

    RefSeq Version XM_009295201.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295201.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGG ATGAAGCAGA CTCCAAAGGT
    GCATCTCCAT GGAAGTCTGT TCGCTTGGCT GTTCAAACAA CGGCCAACTT TAATACTATC
    AAGGAGCTGA ACAACCGCTG GAGGTCTCTG CAGCAGCTGG CTGAAGACCG AAGCAACATG
    CTGGGCAGTG CTCACGAAGT TCAGAGGTTC CACAGAGATG CAGATGAGAC CAAGGAGTGG
    ATCGAAGAGA AGAACCAAGC CCTGAACACT GATAACTATG GCCATGACCT CGCCAGCGTG
    CAGGCTCTGC AGCGCAAGCA TGAAGGATTT GAGAGAGATC TAGCCGCTTT AGGAGACAAG
    GTGAATTCTC TTGGTGAGAC TGCAGAGCGT CTGATTCAAT CTCATCCTGA GGCTGTGGAT
    GACATCCAGG AGAAGTGCAC CGAGCTAAAC ACAGCATGGA GCAGCCTGGT GGGTCGTGCA
    GACCAGCGCA AAGAAAAACT GGGCAACTCC CATGACCTGC AGCGCTTCCT CAGTGACTTC
    AGAGATCTCA TGTCTTGGAT CAATGGAATC AGGGGACTTG TGTCTTCCGA TGAGCTGGCC
    AAGGATGTTA CTGGTGCTGA AGCCCTGTTG GAGAGACATC AGGAACACCG CACTGAGATC
    GACGCTCGTG CAGGAACCTT CCTGGCTTTT GAGCAGTTTG GACAGCAGCT GCTCGCTCGT
    GGGCATTACG CAAGCCCTGA GATCAAACAG AAGCTGGAGG CTCTTGACCG GGAGCGTGCT
    GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ACCAGTGCCT GGAGCTGCAG
    TTGTTTAACC GTGACTGTGA GCAGGCTGAG AACTGGATGG CTGCCCGCGA AGCCTTCTTA
    GCCAGCGATG ACAAGGGAGA TTCTCTGGAC AGTGTGGAGG CGCTCATTAA GAAACACGAG
    GACTTTGATA AGGCTATCAA TGTGCAGGAA GAAAAGATTG CTGCACTGCA GTCTTTTGCT
    GACCAGCTCA TCAGCGCTGA CCACTATGCC AAGCCTGAGA TCTTCAAACG ACGCAGTGAG
    GTCTTGGACA GGTGGCTCCG CCTCAAGGCT CAGATGATTG AGAAACGCTC CAAGCTTGGC
    GAGTCACAGA CTCTACAGCA GTTCAGCAGA GACGTTGACG AGATCGAAGC TTGGATCAGT
    GAGAAACTCC AGACCGCCAC AGACGAGTCT TACAAAGACC CCACCAACAT CCAGCTGTCC
    AAGCTGCTGA GCAAACACCA GAAACACCAG GCTTTTGAGG CTGAGCTACA CGCTAACGCT
    GATCGTATCC GCGGAGTGAT TGACACGGGC AATGCTCTCA TCCAGAGAGG TGCCTGTGCT
    GGAAGTGAAG ATGCTGTTAA GGCTCGTCTG AATGCTCTGG ACGAGCAGTG GCAGTTCCTG
    GTCAACAAGT CTGCTGAGAA GAGTCAGAAA CTGAAGGAGG CCAACAAACA GCAGAACTTC
    AACACTGGCA TCAAGGACTT TGACTTCTGG TTGTCTGAGG TTGAGGCTCT TCTTGCCTCT
    GAGGATTATG GGAAAGATCT GGCTTCAGTC AACAATCTGC TGAAGAAGCA CCAGCTGTTG
    GAGGCTGATA TCTCTGCTCA TGAGGATCGT TTGAAGGATC TTAATGGCCA GGCTGACAGT
    CTGATGGCCA GCAACGCTTT CGACACATCT CAGGTCAAAG ACAAGCGGGA TGCCGTTAAC
    GGACGCTTCG GCAAAATCAA GAGCATGGCA GCGGGACGGC GAGCCAAGCT GAACGAATCG
    CATCGTCTGC ACCAGTTCTT CAGGGATCTG GACGATGAGG AGTCCTGGAT TAAGGAAAAG
    AAGTTGTTGG TGAGCTCGGA GGACTACGGA CGTGATTTGA CAGGGGTGCA GAATCTGAGG
    AAGAAGCACA AGAGGCTGGA GGCTGAGCTG GGAGCACACG AGCCGGCCAT TCAGTCTGTG
    TTGGAGACTG GGAAGAAGCT GTCTGACGAC AACACCATTG GACAGGAGGA GATCCAGCAG
    AGACTGGCCC AGTTTGTGGA GCACTGGAGG GAGCTGAAGG ATCTGGCTGC TGCACGTGGG
    CAGAGGCTTG AAGAGTCGCT GGAATACCAG CAGTTTGTGG CGAACGTGGA AGAAGAGGAA
    GCCTGGATCA ACGAGAAACT GAACCTGGTC GGAAGTGAAG ATTACGGTGA TACTCTGGCA
    GCTGTGCAGG GCTTGCTGAA GAAACATGAA GCATTTGAAA CTGATTTTAC TGTGCACAGG
    GACCGTGTGA ATGATGTGTG TTCCAATGGA GATGAACTCG TTAAAAAGGA GAACCACAAT
    GTGGAGAACA TCATGGCTAA AATGAAGGCG TTGCGTGGAA AAGTGTTGGA GCTGGAGAGA
    GCAGCGGCAC AGAGGAAAGC CAAGCTGGAT GAAAACTCTG CCTTCCTGCA GTTCAACTGG
    AAGGCTGATG TGGTGGAGTC CTGGATTGGT GAGAAGGAAA ACAGCCTGAA GACTGATGAC
    TATGGTCGCG ATCTCTCCTC TGTGCAGACG CTGCTCACCA AACAGGAAAC CTTTGATGCA
    GGACTTCAGG CCTTCCAGCA GGAGGGCATC ACCAACATCA CAGCTCTAAA AGACCAGCTG
    CTGGCAGCCA AACATGTGCA GTCTAAGGCC ATCGAGGCTC GTCACGCCAC CCTGATGAAG
    CGCTGGAACC AACTACTATC CAACTCAGCA GCCCGCAAGA AGAAGCTTCT GGAAGCACAG
    GACCACTTCA GAAAGGTGGA GGATCTTTTC CTCACGTTTG CCAAGAAAGC CTCAGCCTTC
    AACAGCTGGT TTGAGAACGC AGAGGAAGAT CTGACCGATC CTGTGAGGTG CAACTCTCTG
    GAGGAGATTA GGGCACTGCG GGATGCCCAT GATGCCTTCC GCTCGTCCCT GAGCTCCGCA
    GAGGCCGACT TCAGCCAGCT GGCAGAACTC GATCGGCAGA TTAAGAGCTA CAACGTGGTG
    TCCAACCCAT ACACCTGGTT CACCATGGAG GCTCTGGAGG AGACCTGGAG TAACCTTCAG
    AAGATCATCA AGGAGCGAGA ACTGGAGCTG CAGAAGGAGC AGCGCCGACA GGAAGAGAAT
    GATAAACTCA GACAGGAGTT TGCTCAGCAT GCTAATGCTT TCCACCAATG GCTGCAGGAG
    ACCAGGACAT ACCTTCTGGA TGGGTCCTGC ATGGTGGAAG AATCTGGAAC TCTAGAGTCA
    CAGCTAGAAG CCACTAAGCG TAAGCATCAG GAGATTCGTG CCATGCGCAG TCAGCTGAAG
    AAGATTGAGG ATCTGGGAGC TGCCATGGAG GAGGCGCTGA TTCTGGACAA TAAATACACA
    GAACACAGCA CCGTGGGTCT GGCACAACAG TGGGACCAAC TCGACCAGCT GGGCATGAGA
    ATGCAACATA ACCTGGAACA ACAGATCCAA GCCAGAAACA CTACAGGTGT AACAGAAGAG
    GCCCTGAAGG AGTTCAGCAT GATGTTCAAG CACTTCGACA AAGAAAAATC TGGCCGTCTG
    AATCACCAGG AGTTCAAGTC TTGTCTGCGG TCTCTGGGTT ACGACCTTCC CATGGTGGAA
    GAAGGAGAAC CAGACCCAGA GTTTGAGTCC ATCCTGGATA TAGTGGATCC AAACAGGGAT
    GGAAACGTGT CCTTGCAGGA GTACATGGCT TTCATGATCA GCCGAGAAAC GGAGAACGTC
    AAGTCAAGCG AGGAGATCGA GAGCGCTTTC CGTGCTCTGA GCGCAGAAAA CAAACCCTAC
    GTCACCAAGG AAGAACTCTA CCAGAACCTG ACAAAGGAAC AAGCCGACTA CTGCATTTCC
    CATATGAAGC CCTACCTAGA CAGCAAGGGC CGCGAAATCC CATCAGCTTT CGACTTTGTG
    GAATTCACCC GCTCGCTCTT CGTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41936
    Clone ID Related Accession (Same CDS sequence) XM_009295202.2
    Accession Version XM_009295202.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGGA GCTGAACAAC
    CGCTGGAGGT CTCTGCAGCA GCTGGCTGAA GACCGAAGCA ACATGCTGGG CAGTGCTCAC
    GAAGTTCAGA GGTTCCACAG AGATGCAGAT GAGACCAAGG AGTGGATCGA AGAGAAGAAC
    CAAGCCCTGA ACACTGATAA CTATGGCCAT GACCTCGCCA GCGTGCAGGC TCTGCAGCGC
    AAGCATGAAG GATTTGAGAG AGATCTAGCC GCTTTAGGAG ACAAGGTGAA TTCTCTTGGT
    GAGACTGCAG AGCGTCTGAT TCAATCTCAT CCTGAGGCTG TGGATGACAT CCAGGAGAAG
    TGCACCGAGC TAAACACAGC ATGGAGCAGC CTGGTGGGTC GTGCAGACCA GCGCAAAGAA
    AAACTGGGCA ACTCCCATGA CCTGCAGCGC TTCCTCAGTG ACTTCAGAGA TCTCATGTCT
    TGGATCAATG GAATCAGGGG ACTTGTGTCT TCCGATGAGC TGGCCAAGGA TGTTACTGGT
    GCTGAAGCCC TGTTGGAGAG ACATCAGGAA CACCGCACTG AGATCGACGC TCGTGCAGGA
    ACCTTCCTGG CTTTTGAGCA GTTTGGACAG CAGCTGCTCG CTCGTGGGCA TTACGCAAGC
    CCTGAGATCA AACAGAAGCT GGAGGCTCTT GACCGGGAGC GTGCTGACCT GGAGAAGGCC
    TGGGTTCAGC GCAGGATGAT GCTGGACCAG TGCCTGGAGC TGCAGTTGTT TAACCGTGAC
    TGTGAGCAGG CTGAGAACTG GATGGCTGCC CGCGAAGCCT TCTTAGCCAG CGATGACAAG
    GGAGATTCTC TGGACAGTGT GGAGGCGCTC ATTAAGAAAC ACGAGGACTT TGATAAGGCT
    ATCAATGTGC AGGAAGAAAA GATTGCTGCA CTGCAGTCTT TTGCTGACCA GCTCATCAGC
    GCTGACCACT ATGCCAAGCC TGAGATCTTC AAACGACGCA GTGAGGTCTT GGACAGGTGG
    CTCCGCCTCA AGGCTCAGAT GATTGAGAAA CGCTCCAAGC TTGGCGAGTC ACAGACTCTA
    CAGCAGTTCA GCAGAGACGT TGACGAGATC GAAGCTTGGA TCAGTGAGAA ACTCCAGACC
    GCCACAGACG AGTCTTACAA AGACCCCACC AACATCCAGC TGTCCAAGCT GCTGAGCAAA
    CACCAGAAAC ACCAGGCTTT TGAGGCTGAG CTACACGCTA ACGCTGATCG TATCCGCGGA
    GTGATTGACA CGGGCAATGC TCTCATCCAG AGAGGTGCCT GTGCTGGAAG TGAAGATGCT
    GTTAAGGCTC GTCTGAATGC TCTGGACGAG CAGTGGCAGT TCCTGGTCAA CAAGTCTGCT
    GAGAAGAGTC AGAAACTGAA GGAGGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG
    GACTTTGACT TCTGGTTGTC TGAGGTTGAG GCTCTTCTTG CCTCTGAGGA TTATGGGAAA
    GATCTGGCTT CAGTCAACAA TCTGCTGAAG AAGCACCAGC TGTTGGAGGC TGATATCTCT
    GCTCATGAGG ATCGTTTGAA GGATCTTAAT GGCCAGGCTG ACAGTCTGAT GGCCAGCAAC
    GCTTTCGACA CATCTCAGGT CAAAGACAAG CGGGATGCCG TTAACGGACG CTTCGGCAAA
    ATCAAGAGCA TGGCAGCGGG ACGGCGAGCC AAGCTGAACG AATCGCATCG TCTGCACCAG
    TTCTTCAGGG ATCTGGACGA TGAGGAGTCC TGGATTAAGG AAAAGAAGTT GTTGGTGAGC
    TCGGAGGACT ACGGACGTGA TTTGACAGGG GTGCAGAATC TGAGGAAGAA GCACAAGAGG
    CTGGAGGCTG AGCTGGGAGC ACACGAGCCG GCCATTCAGT CTGTGTTGGA GACTGGGAAG
    AAGCTGTCTG ACGACAACAC CATTGGACAG GAGGAGATCC AGCAGAGACT GGCCCAGTTT
    GTGGAGCACT GGAGGGAGCT GAAGGATCTG GCTGCTGCAC GTGGGCAGAG GCTTGAAGAG
    TCGCTGGAAT ACCAGCAGTT TGTGGCGAAC GTGGAAGAAG AGGAAGCCTG GATCAACGAG
    AAACTGAACC TGGTCGGAAG TGAAGATTAC GGTGATACTC TGGCAGCTGT GCAGGGCTTG
    CTGAAGAAAC ATGAAGCATT TGAAACTGAT TTTACTGTGC ACAGGGACCG TGTGAATGAT
    GTGTGTTCCA ATGGAGATGA ACTCGTTAAA AAGGAGAACC ACAATGTGGA GAACATCATG
    GCTAAAATGA AGGCGTTGCG TGGAAAAGTG TTGGAGCTGG AGAGAGCAGC GGCACAGAGG
    AAAGCCAAGC TGGATGAAAA CTCTGCCTTC CTGCAGTTCA ACTGGAAGGC TGATGTGGTG
    GAGTCCTGGA TTGGTGAGAA GGAAAACAGC CTGAAGACTG ATGACTATGG TCGCGATCTC
    TCCTCTGTGC AGACGCTGCT CACCAAACAG GAAACCTTTG ATGCAGGACT TCAGGCCTTC
    CAGCAGGAGG GCATCACCAA CATCACAGCT CTAAAAGACC AGCTGCTGGC AGCCAAACAT
    GTGCAGTCTA AGGCCATCGA GGCTCGTCAC GCCACCCTGA TGAAGCGCTG GAACCAACTA
    CTATCCAACT CAGCAGCCCG CAAGAAGAAG CTTCTGGAAG CACAGGACCA CTTCAGAAAG
    GTGGAGGATC TTTTCCTCAC GTTTGCCAAG AAAGCCTCAG CCTTCAACAG CTGGTTTGAG
    AACGCAGAGG AAGATCTGAC CGATCCTGTG AGGTGCAACT CTCTGGAGGA GATTAGGGCA
    CTGCGGGATG CCCATGATGC CTTCCGCTCG TCCCTGAGCT CCGCAGAGGC CGACTTCAGC
    CAGCTGGCAG AACTCGATCG GCAGATTAAG AGCTACAACG TGGTGTCCAA CCCATACACC
    TGGTTCACCA TGGAGGCTCT GGAGGAGACC TGGAGTAACC TTCAGAAGAT CATCAAGGAG
    CGAGAACTGG AGCTGCAGAA GGAGCAGCGC CGACAGGAAG AGAATGATAA ACTCAGACAG
    GAGTTTGCTC AGCATGCTAA TGCTTTCCAC CAATGGCTGC AGGAGACCAG GACATACCTT
    CTGGATGGGT CCTGCATGGT GGAAGAATCT GGAACTCTAG AGTCACAGCT AGAAGCCACT
    AAGCGTAAGC ATCAGGAGAT TCGTGCCATG CGCAGTCAGC TGAAGAAGAT TGAGGATCTG
    GGAGCTGCCA TGGAGGAGGC GCTGATTCTG GACAATAAAT ACACAGAACA CAGCACCGTG
    GGTCTGGCAC AACAGTGGGA CCAACTCGAC CAGCTGGGCA TGAGAATGCA ACATAACCTG
    GAACAACAGA TCCAAGCCAG AAACACTACA GGTGTAACAG AAGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT CGACAAAGAA AAATCTGGCC GTCTGAATCA CCAGGAGTTC
    AAGTCTTGTC TGCGGTCTCT GGGTTACGAC CTTCCCATGG TGGAAGAAGG AGAACCAGAC
    CCAGAGTTTG AGTCCATCCT GGATATAGTG GATCCAAACA GGGATGGAAA CGTGTCCTTG
    CAGGAGTACA TGGCTTTCAT GATCAGCCGA GAAACGGAGA ACGTCAAGTC AAGCGAGGAG
    ATCGAGAGCG CTTTCCGTGC TCTGAGCGCA GAAAACAAAC CCTACGTCAC CAAGGAAGAA
    CTCTACCAGA ACCTGACAAA GGAACAAGCC GACTACTGCA TTTCCCATAT GAAGCCCTAC
    CTAGACAGCA AGGGCCGCGA AATCCCATCA GCTTTCGACT TTGTGGAATT CACCCGCTCG
    CTCTTCGTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293477.1
    CDS114..7508
    Translation

    Target ORF information:

    RefSeq Version XM_009295202.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295202.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGGA GCTGAACAAC
    CGCTGGAGGT CTCTGCAGCA GCTGGCTGAA GACCGAAGCA ACATGCTGGG CAGTGCTCAC
    GAAGTTCAGA GGTTCCACAG AGATGCAGAT GAGACCAAGG AGTGGATCGA AGAGAAGAAC
    CAAGCCCTGA ACACTGATAA CTATGGCCAT GACCTCGCCA GCGTGCAGGC TCTGCAGCGC
    AAGCATGAAG GATTTGAGAG AGATCTAGCC GCTTTAGGAG ACAAGGTGAA TTCTCTTGGT
    GAGACTGCAG AGCGTCTGAT TCAATCTCAT CCTGAGGCTG TGGATGACAT CCAGGAGAAG
    TGCACCGAGC TAAACACAGC ATGGAGCAGC CTGGTGGGTC GTGCAGACCA GCGCAAAGAA
    AAACTGGGCA ACTCCCATGA CCTGCAGCGC TTCCTCAGTG ACTTCAGAGA TCTCATGTCT
    TGGATCAATG GAATCAGGGG ACTTGTGTCT TCCGATGAGC TGGCCAAGGA TGTTACTGGT
    GCTGAAGCCC TGTTGGAGAG ACATCAGGAA CACCGCACTG AGATCGACGC TCGTGCAGGA
    ACCTTCCTGG CTTTTGAGCA GTTTGGACAG CAGCTGCTCG CTCGTGGGCA TTACGCAAGC
    CCTGAGATCA AACAGAAGCT GGAGGCTCTT GACCGGGAGC GTGCTGACCT GGAGAAGGCC
    TGGGTTCAGC GCAGGATGAT GCTGGACCAG TGCCTGGAGC TGCAGTTGTT TAACCGTGAC
    TGTGAGCAGG CTGAGAACTG GATGGCTGCC CGCGAAGCCT TCTTAGCCAG CGATGACAAG
    GGAGATTCTC TGGACAGTGT GGAGGCGCTC ATTAAGAAAC ACGAGGACTT TGATAAGGCT
    ATCAATGTGC AGGAAGAAAA GATTGCTGCA CTGCAGTCTT TTGCTGACCA GCTCATCAGC
    GCTGACCACT ATGCCAAGCC TGAGATCTTC AAACGACGCA GTGAGGTCTT GGACAGGTGG
    CTCCGCCTCA AGGCTCAGAT GATTGAGAAA CGCTCCAAGC TTGGCGAGTC ACAGACTCTA
    CAGCAGTTCA GCAGAGACGT TGACGAGATC GAAGCTTGGA TCAGTGAGAA ACTCCAGACC
    GCCACAGACG AGTCTTACAA AGACCCCACC AACATCCAGC TGTCCAAGCT GCTGAGCAAA
    CACCAGAAAC ACCAGGCTTT TGAGGCTGAG CTACACGCTA ACGCTGATCG TATCCGCGGA
    GTGATTGACA CGGGCAATGC TCTCATCCAG AGAGGTGCCT GTGCTGGAAG TGAAGATGCT
    GTTAAGGCTC GTCTGAATGC TCTGGACGAG CAGTGGCAGT TCCTGGTCAA CAAGTCTGCT
    GAGAAGAGTC AGAAACTGAA GGAGGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG
    GACTTTGACT TCTGGTTGTC TGAGGTTGAG GCTCTTCTTG CCTCTGAGGA TTATGGGAAA
    GATCTGGCTT CAGTCAACAA TCTGCTGAAG AAGCACCAGC TGTTGGAGGC TGATATCTCT
    GCTCATGAGG ATCGTTTGAA GGATCTTAAT GGCCAGGCTG ACAGTCTGAT GGCCAGCAAC
    GCTTTCGACA CATCTCAGGT CAAAGACAAG CGGGATGCCG TTAACGGACG CTTCGGCAAA
    ATCAAGAGCA TGGCAGCGGG ACGGCGAGCC AAGCTGAACG AATCGCATCG TCTGCACCAG
    TTCTTCAGGG ATCTGGACGA TGAGGAGTCC TGGATTAAGG AAAAGAAGTT GTTGGTGAGC
    TCGGAGGACT ACGGACGTGA TTTGACAGGG GTGCAGAATC TGAGGAAGAA GCACAAGAGG
    CTGGAGGCTG AGCTGGGAGC ACACGAGCCG GCCATTCAGT CTGTGTTGGA GACTGGGAAG
    AAGCTGTCTG ACGACAACAC CATTGGACAG GAGGAGATCC AGCAGAGACT GGCCCAGTTT
    GTGGAGCACT GGAGGGAGCT GAAGGATCTG GCTGCTGCAC GTGGGCAGAG GCTTGAAGAG
    TCGCTGGAAT ACCAGCAGTT TGTGGCGAAC GTGGAAGAAG AGGAAGCCTG GATCAACGAG
    AAACTGAACC TGGTCGGAAG TGAAGATTAC GGTGATACTC TGGCAGCTGT GCAGGGCTTG
    CTGAAGAAAC ATGAAGCATT TGAAACTGAT TTTACTGTGC ACAGGGACCG TGTGAATGAT
    GTGTGTTCCA ATGGAGATGA ACTCGTTAAA AAGGAGAACC ACAATGTGGA GAACATCATG
    GCTAAAATGA AGGCGTTGCG TGGAAAAGTG TTGGAGCTGG AGAGAGCAGC GGCACAGAGG
    AAAGCCAAGC TGGATGAAAA CTCTGCCTTC CTGCAGTTCA ACTGGAAGGC TGATGTGGTG
    GAGTCCTGGA TTGGTGAGAA GGAAAACAGC CTGAAGACTG ATGACTATGG TCGCGATCTC
    TCCTCTGTGC AGACGCTGCT CACCAAACAG GAAACCTTTG ATGCAGGACT TCAGGCCTTC
    CAGCAGGAGG GCATCACCAA CATCACAGCT CTAAAAGACC AGCTGCTGGC AGCCAAACAT
    GTGCAGTCTA AGGCCATCGA GGCTCGTCAC GCCACCCTGA TGAAGCGCTG GAACCAACTA
    CTATCCAACT CAGCAGCCCG CAAGAAGAAG CTTCTGGAAG CACAGGACCA CTTCAGAAAG
    GTGGAGGATC TTTTCCTCAC GTTTGCCAAG AAAGCCTCAG CCTTCAACAG CTGGTTTGAG
    AACGCAGAGG AAGATCTGAC CGATCCTGTG AGGTGCAACT CTCTGGAGGA GATTAGGGCA
    CTGCGGGATG CCCATGATGC CTTCCGCTCG TCCCTGAGCT CCGCAGAGGC CGACTTCAGC
    CAGCTGGCAG AACTCGATCG GCAGATTAAG AGCTACAACG TGGTGTCCAA CCCATACACC
    TGGTTCACCA TGGAGGCTCT GGAGGAGACC TGGAGTAACC TTCAGAAGAT CATCAAGGAG
    CGAGAACTGG AGCTGCAGAA GGAGCAGCGC CGACAGGAAG AGAATGATAA ACTCAGACAG
    GAGTTTGCTC AGCATGCTAA TGCTTTCCAC CAATGGCTGC AGGAGACCAG GACATACCTT
    CTGGATGGGT CCTGCATGGT GGAAGAATCT GGAACTCTAG AGTCACAGCT AGAAGCCACT
    AAGCGTAAGC ATCAGGAGAT TCGTGCCATG CGCAGTCAGC TGAAGAAGAT TGAGGATCTG
    GGAGCTGCCA TGGAGGAGGC GCTGATTCTG GACAATAAAT ACACAGAACA CAGCACCGTG
    GGTCTGGCAC AACAGTGGGA CCAACTCGAC CAGCTGGGCA TGAGAATGCA ACATAACCTG
    GAACAACAGA TCCAAGCCAG AAACACTACA GGTGTAACAG AAGAGGCCCT GAAGGAGTTC
    AGCATGATGT TCAAGCACTT CGACAAAGAA AAATCTGGCC GTCTGAATCA CCAGGAGTTC
    AAGTCTTGTC TGCGGTCTCT GGGTTACGAC CTTCCCATGG TGGAAGAAGG AGAACCAGAC
    CCAGAGTTTG AGTCCATCCT GGATATAGTG GATCCAAACA GGGATGGAAA CGTGTCCTTG
    CAGGAGTACA TGGCTTTCAT GATCAGCCGA GAAACGGAGA ACGTCAAGTC AAGCGAGGAG
    ATCGAGAGCG CTTTCCGTGC TCTGAGCGCA GAAAACAAAC CCTACGTCAC CAAGGAAGAA
    CTCTACCAGA ACCTGACAAA GGAACAAGCC GACTACTGCA TTTCCCATAT GAAGCCCTAC
    CTAGACAGCA AGGGCCGCGA AATCCCATCA GCTTTCGACT TTGTGGAATT CACCCGCTCG
    CTCTTCGTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41937
    Clone ID Related Accession (Same CDS sequence) XM_021468833.1 , XM_009295203.2
    Accession Version XM_021468833.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGGTCC
    TGCATGGTGG AAGAATCTGG AACTCTAGAG TCACAGCTAG AAGCCACTAA GCGTAAGCAT
    CAGGAGATTC GTGCCATGCG CAGTCAGCTG AAGAAGATTG AGGATCTGGG AGCTGCCATG
    GAGGAGGCGC TGATTCTGGA CAATAAATAC ACAGAACACA GCACCGTGGG TCTGGCACAA
    CAGTGGGACC AACTCGACCA GCTGGGCATG AGAATGCAAC ATAACCTGGA ACAACAGATC
    CAAGCCAGAA ACACTACAGG TGTAACAGAA GAGGCCCTGA AGGAGTTCAG CATGATGTTC
    AAGCACTTCG ACAAAGAAAA ATCTGGCCGT CTGAATCACC AGGAGTTCAA GTCTTGTCTG
    CGGTCTCTGG GTTACGACCT TCCCATGGTG GAAGAAGGAG AACCAGACCC AGAGTTTGAG
    TCCATCCTGG ATATAGTGGA TCCAAACAGG GATGGAAACG TGTCCTTGCA GGAGTACATG
    GCTTTCATGA TCAGCCGAGA AACGGAGAAC GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT
    TTCCGTGCTC TGAGCGCAGA AAACAAACCC TACGTCACCA AGGAAGAACT CTACCAGAAC
    CTGACAAAGG AACAAGCCGA CTACTGCATT TCCCATATGA AGCCCTACCT AGACAGCAAG
    GGCCGCGAAA TCCCATCAGC TTTCGACTTT GTGGAATTCA CCCGCTCGCT CTTCGTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324508.1
    CDS4..7386
    Translation

    Target ORF information:

    RefSeq Version XM_021468833.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468833.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGGTCC
    TGCATGGTGG AAGAATCTGG AACTCTAGAG TCACAGCTAG AAGCCACTAA GCGTAAGCAT
    CAGGAGATTC GTGCCATGCG CAGTCAGCTG AAGAAGATTG AGGATCTGGG AGCTGCCATG
    GAGGAGGCGC TGATTCTGGA CAATAAATAC ACAGAACACA GCACCGTGGG TCTGGCACAA
    CAGTGGGACC AACTCGACCA GCTGGGCATG AGAATGCAAC ATAACCTGGA ACAACAGATC
    CAAGCCAGAA ACACTACAGG TGTAACAGAA GAGGCCCTGA AGGAGTTCAG CATGATGTTC
    AAGCACTTCG ACAAAGAAAA ATCTGGCCGT CTGAATCACC AGGAGTTCAA GTCTTGTCTG
    CGGTCTCTGG GTTACGACCT TCCCATGGTG GAAGAAGGAG AACCAGACCC AGAGTTTGAG
    TCCATCCTGG ATATAGTGGA TCCAAACAGG GATGGAAACG TGTCCTTGCA GGAGTACATG
    GCTTTCATGA TCAGCCGAGA AACGGAGAAC GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT
    TTCCGTGCTC TGAGCGCAGA AAACAAACCC TACGTCACCA AGGAAGAACT CTACCAGAAC
    CTGACAAAGG AACAAGCCGA CTACTGCATT TCCCATATGA AGCCCTACCT AGACAGCAAG
    GGCCGCGAAA TCCCATCAGC TTTCGACTTT GTGGAATTCA CCCGCTCGCT CTTCGTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41937
    Clone ID Related Accession (Same CDS sequence) XM_021468833.1 , XM_009295203.2
    Accession Version XM_009295203.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGGTCC
    TGCATGGTGG AAGAATCTGG AACTCTAGAG TCACAGCTAG AAGCCACTAA GCGTAAGCAT
    CAGGAGATTC GTGCCATGCG CAGTCAGCTG AAGAAGATTG AGGATCTGGG AGCTGCCATG
    GAGGAGGCGC TGATTCTGGA CAATAAATAC ACAGAACACA GCACCGTGGG TCTGGCACAA
    CAGTGGGACC AACTCGACCA GCTGGGCATG AGAATGCAAC ATAACCTGGA ACAACAGATC
    CAAGCCAGAA ACACTACAGG TGTAACAGAA GAGGCCCTGA AGGAGTTCAG CATGATGTTC
    AAGCACTTCG ACAAAGAAAA ATCTGGCCGT CTGAATCACC AGGAGTTCAA GTCTTGTCTG
    CGGTCTCTGG GTTACGACCT TCCCATGGTG GAAGAAGGAG AACCAGACCC AGAGTTTGAG
    TCCATCCTGG ATATAGTGGA TCCAAACAGG GATGGAAACG TGTCCTTGCA GGAGTACATG
    GCTTTCATGA TCAGCCGAGA AACGGAGAAC GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT
    TTCCGTGCTC TGAGCGCAGA AAACAAACCC TACGTCACCA AGGAAGAACT CTACCAGAAC
    CTGACAAAGG AACAAGCCGA CTACTGCATT TCCCATATGA AGCCCTACCT AGACAGCAAG
    GGCCGCGAAA TCCCATCAGC TTTCGACTTT GTGGAATTCA CCCGCTCGCT CTTCGTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293478.1
    CDS114..7496
    Translation

    Target ORF information:

    RefSeq Version XM_009295203.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295203.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGCTG TCCAAGCTGC TGAGCAAACA CCAGAAACAC
    CAGGCTTTTG AGGCTGAGCT ACACGCTAAC GCTGATCGTA TCCGCGGAGT GATTGACACG
    GGCAATGCTC TCATCCAGAG AGGTGCCTGT GCTGGAAGTG AAGATGCTGT TAAGGCTCGT
    CTGAATGCTC TGGACGAGCA GTGGCAGTTC CTGGTCAACA AGTCTGCTGA GAAGAGTCAG
    AAACTGAAGG AGGCCAACAA ACAGCAGAAC TTCAACACTG GCATCAAGGA CTTTGACTTC
    TGGTTGTCTG AGGTTGAGGC TCTTCTTGCC TCTGAGGATT ATGGGAAAGA TCTGGCTTCA
    GTCAACAATC TGCTGAAGAA GCACCAGCTG TTGGAGGCTG ATATCTCTGC TCATGAGGAT
    CGTTTGAAGG ATCTTAATGG CCAGGCTGAC AGTCTGATGG CCAGCAACGC TTTCGACACA
    TCTCAGGTCA AAGACAAGCG GGATGCCGTT AACGGACGCT TCGGCAAAAT CAAGAGCATG
    GCAGCGGGAC GGCGAGCCAA GCTGAACGAA TCGCATCGTC TGCACCAGTT CTTCAGGGAT
    CTGGACGATG AGGAGTCCTG GATTAAGGAA AAGAAGTTGT TGGTGAGCTC GGAGGACTAC
    GGACGTGATT TGACAGGGGT GCAGAATCTG AGGAAGAAGC ACAAGAGGCT GGAGGCTGAG
    CTGGGAGCAC ACGAGCCGGC CATTCAGTCT GTGTTGGAGA CTGGGAAGAA GCTGTCTGAC
    GACAACACCA TTGGACAGGA GGAGATCCAG CAGAGACTGG CCCAGTTTGT GGAGCACTGG
    AGGGAGCTGA AGGATCTGGC TGCTGCACGT GGGCAGAGGC TTGAAGAGTC GCTGGAATAC
    CAGCAGTTTG TGGCGAACGT GGAAGAAGAG GAAGCCTGGA TCAACGAGAA ACTGAACCTG
    GTCGGAAGTG AAGATTACGG TGATACTCTG GCAGCTGTGC AGGGCTTGCT GAAGAAACAT
    GAAGCATTTG AAACTGATTT TACTGTGCAC AGGGACCGTG TGAATGATGT GTGTTCCAAT
    GGAGATGAAC TCGTTAAAAA GGAGAACCAC AATGTGGAGA ACATCATGGC TAAAATGAAG
    GCGTTGCGTG GAAAAGTGTT GGAGCTGGAG AGAGCAGCGG CACAGAGGAA AGCCAAGCTG
    GATGAAAACT CTGCCTTCCT GCAGTTCAAC TGGAAGGCTG ATGTGGTGGA GTCCTGGATT
    GGTGAGAAGG AAAACAGCCT GAAGACTGAT GACTATGGTC GCGATCTCTC CTCTGTGCAG
    ACGCTGCTCA CCAAACAGGA AACCTTTGAT GCAGGACTTC AGGCCTTCCA GCAGGAGGGC
    ATCACCAACA TCACAGCTCT AAAAGACCAG CTGCTGGCAG CCAAACATGT GCAGTCTAAG
    GCCATCGAGG CTCGTCACGC CACCCTGATG AAGCGCTGGA ACCAACTACT ATCCAACTCA
    GCAGCCCGCA AGAAGAAGCT TCTGGAAGCA CAGGACCACT TCAGAAAGGT GGAGGATCTT
    TTCCTCACGT TTGCCAAGAA AGCCTCAGCC TTCAACAGCT GGTTTGAGAA CGCAGAGGAA
    GATCTGACCG ATCCTGTGAG GTGCAACTCT CTGGAGGAGA TTAGGGCACT GCGGGATGCC
    CATGATGCCT TCCGCTCGTC CCTGAGCTCC GCAGAGGCCG ACTTCAGCCA GCTGGCAGAA
    CTCGATCGGC AGATTAAGAG CTACAACGTG GTGTCCAACC CATACACCTG GTTCACCATG
    GAGGCTCTGG AGGAGACCTG GAGTAACCTT CAGAAGATCA TCAAGGAGCG AGAACTGGAG
    CTGCAGAAGG AGCAGCGCCG ACAGGAAGAG AATGATAAAC TCAGACAGGA GTTTGCTCAG
    CATGCTAATG CTTTCCACCA ATGGCTGCAG GAGACCAGGA CATACCTTCT GGATGGGTCC
    TGCATGGTGG AAGAATCTGG AACTCTAGAG TCACAGCTAG AAGCCACTAA GCGTAAGCAT
    CAGGAGATTC GTGCCATGCG CAGTCAGCTG AAGAAGATTG AGGATCTGGG AGCTGCCATG
    GAGGAGGCGC TGATTCTGGA CAATAAATAC ACAGAACACA GCACCGTGGG TCTGGCACAA
    CAGTGGGACC AACTCGACCA GCTGGGCATG AGAATGCAAC ATAACCTGGA ACAACAGATC
    CAAGCCAGAA ACACTACAGG TGTAACAGAA GAGGCCCTGA AGGAGTTCAG CATGATGTTC
    AAGCACTTCG ACAAAGAAAA ATCTGGCCGT CTGAATCACC AGGAGTTCAA GTCTTGTCTG
    CGGTCTCTGG GTTACGACCT TCCCATGGTG GAAGAAGGAG AACCAGACCC AGAGTTTGAG
    TCCATCCTGG ATATAGTGGA TCCAAACAGG GATGGAAACG TGTCCTTGCA GGAGTACATG
    GCTTTCATGA TCAGCCGAGA AACGGAGAAC GTCAAGTCAA GCGAGGAGAT CGAGAGCGCT
    TTCCGTGCTC TGAGCGCAGA AAACAAACCC TACGTCACCA AGGAAGAACT CTACCAGAAC
    CTGACAAAGG AACAAGCCGA CTACTGCATT TCCCATATGA AGCCCTACCT AGACAGCAAG
    GGCCGCGAAA TCCCATCAGC TTTCGACTTT GTGGAATTCA CCCGCTCGCT CTTCGTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41938
    Clone ID Related Accession (Same CDS sequence) XM_009295204.2
    Accession Version XM_009295204.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGG ATGAAGCAGA CTCCAAAGGT
    GCATCTCCAT GGAAGGAGCT GAACAACCGC TGGAGGTCTC TGCAGCAGCT GGCTGAAGAC
    CGAAGCAACA TGCTGGGCAG TGCTCACGAA GTTCAGAGGT TCCACAGAGA TGCAGATGAG
    ACCAAGGAGT GGATCGAAGA GAAGAACCAA GCCCTGAACA CTGATAACTA TGGCCATGAC
    CTCGCCAGCG TGCAGGCTCT GCAGCGCAAG CATGAAGGAT TTGAGAGAGA TCTAGCCGCT
    TTAGGAGACA AGGTGAATTC TCTTGGTGAG ACTGCAGAGC GTCTGATTCA ATCTCATCCT
    GAGGCTGTGG ATGACATCCA GGAGAAGTGC ACCGAGCTAA ACACAGCATG GAGCAGCCTG
    GTGGGTCGTG CAGACCAGCG CAAAGAAAAA CTGGGCAACT CCCATGACCT GCAGCGCTTC
    CTCAGTGACT TCAGAGATCT CATGTCTTGG ATCAATGGAA TCAGGGGACT TGTGTCTTCC
    GATGAGCTGG CCAAGGATGT TACTGGTGCT GAAGCCCTGT TGGAGAGACA TCAGGAACAC
    CGCACTGAGA TCGACGCTCG TGCAGGAACC TTCCTGGCTT TTGAGCAGTT TGGACAGCAG
    CTGCTCGCTC GTGGGCATTA CGCAAGCCCT GAGATCAAAC AGAAGCTGGA GGCTCTTGAC
    CGGGAGCGTG CTGACCTGGA GAAGGCCTGG GTTCAGCGCA GGATGATGCT GGACCAGTGC
    CTGGAGCTGC AGTTGTTTAA CCGTGACTGT GAGCAGGCTG AGAACTGGAT GGCTGCCCGC
    GAAGCCTTCT TAGCCAGCGA TGACAAGGGA GATTCTCTGG ACAGTGTGGA GGCGCTCATT
    AAGAAACACG AGGACTTTGA TAAGGCTATC AATGTGCAGG AAGAAAAGAT TGCTGCACTG
    CAGTCTTTTG CTGACCAGCT CATCAGCGCT GACCACTATG CCAAGCCTGA GATCTTCAAA
    CGACGCAGTG AGGTCTTGGA CAGGTGGCTC CGCCTCAAGG CTCAGATGAT TGAGAAACGC
    TCCAAGCTTG GCGAGTCACA GACTCTACAG CAGTTCAGCA GAGACGTTGA CGAGATCGAA
    GCTTGGATCA GTGAGAAACT CCAGACCGCC ACAGACGAGT CTTACAAAGA CCCCACCAAC
    ATCCAGCTGT CCAAGCTGCT GAGCAAACAC CAGAAACACC AGGCTTTTGA GGCTGAGCTA
    CACGCTAACG CTGATCGTAT CCGCGGAGTG ATTGACACGG GCAATGCTCT CATCCAGAGA
    GGTGCCTGTG CTGGAAGTGA AGATGCTGTT AAGGCTCGTC TGAATGCTCT GGACGAGCAG
    TGGCAGTTCC TGGTCAACAA GTCTGCTGAG AAGAGTCAGA AACTGAAGGA GGCCAACAAA
    CAGCAGAACT TCAACACTGG CATCAAGGAC TTTGACTTCT GGTTGTCTGA GGTTGAGGCT
    CTTCTTGCCT CTGAGGATTA TGGGAAAGAT CTGGCTTCAG TCAACAATCT GCTGAAGAAG
    CACCAGCTGT TGGAGGCTGA TATCTCTGCT CATGAGGATC GTTTGAAGGA TCTTAATGGC
    CAGGCTGACA GTCTGATGGC CAGCAACGCT TTCGACACAT CTCAGGTCAA AGACAAGCGG
    GATGCCGTTA ACGGACGCTT CGGCAAAATC AAGAGCATGG CAGCGGGACG GCGAGCCAAG
    CTGAACGAAT CGCATCGTCT GCACCAGTTC TTCAGGGATC TGGACGATGA GGAGTCCTGG
    ATTAAGGAAA AGAAGTTGTT GGTGAGCTCG GAGGACTACG GACGTGATTT GACAGGGGTG
    CAGAATCTGA GGAAGAAGCA CAAGAGGCTG GAGGCTGAGC TGGGAGCACA CGAGCCGGCC
    ATTCAGTCTG TGTTGGAGAC TGGGAAGAAG CTGTCTGACG ACAACACCAT TGGACAGGAG
    GAGATCCAGC AGAGACTGGC CCAGTTTGTG GAGCACTGGA GGGAGCTGAA GGATCTGGCT
    GCTGCACGTG GGCAGAGGCT TGAAGAGTCG CTGGAATACC AGCAGTTTGT GGCGAACGTG
    GAAGAAGAGG AAGCCTGGAT CAACGAGAAA CTGAACCTGG TCGGAAGTGA AGATTACGGT
    GATACTCTGG CAGCTGTGCA GGGCTTGCTG AAGAAACATG AAGCATTTGA AACTGATTTT
    ACTGTGCACA GGGACCGTGT GAATGATGTG TGTTCCAATG GAGATGAACT CGTTAAAAAG
    GAGAACCACA ATGTGGAGAA CATCATGGCT AAAATGAAGG CGTTGCGTGG AAAAGTGTTG
    GAGCTGGAGA GAGCAGCGGC ACAGAGGAAA GCCAAGCTGG ATGAAAACTC TGCCTTCCTG
    CAGTTCAACT GGAAGGCTGA TGTGGTGGAG TCCTGGATTG GTGAGAAGGA AAACAGCCTG
    AAGACTGATG ACTATGGTCG CGATCTCTCC TCTGTGCAGA CGCTGCTCAC CAAACAGGAA
    ACCTTTGATG CAGGACTTCA GGCCTTCCAG CAGGAGGGCA TCACCAACAT CACAGCTCTA
    AAAGACCAGC TGCTGGCAGC CAAACATGTG CAGTCTAAGG CCATCGAGGC TCGTCACGCC
    ACCCTGATGA AGCGCTGGAA CCAACTACTA TCCAACTCAG CAGCCCGCAA GAAGAAGCTT
    CTGGAAGCAC AGGACCACTT CAGAAAGGTG GAGGATCTTT TCCTCACGTT TGCCAAGAAA
    GCCTCAGCCT TCAACAGCTG GTTTGAGAAC GCAGAGGAAG ATCTGACCGA TCCTGTGAGG
    TGCAACTCTC TGGAGGAGAT TAGGGCACTG CGGGATGCCC ATGATGCCTT CCGCTCGTCC
    CTGAGCTCCG CAGAGGCCGA CTTCAGCCAG CTGGCAGAAC TCGATCGGCA GATTAAGAGC
    TACAACGTGG TGTCCAACCC ATACACCTGG TTCACCATGG AGGCTCTGGA GGAGACCTGG
    AGTAACCTTC AGAAGATCAT CAAGGAGCGA GAACTGGAGC TGCAGAAGGA GCAGCGCCGA
    CAGGAAGAGA ATGATAAACT CAGACAGGAG TTTGCTCAGC ATGCTAATGC TTTCCACCAA
    TGGCTGCAGG AGACCAGGAC ATACCTTCTG GATGGGTCCT GCATGGTGGA AGAATCTGGA
    ACTCTAGAGT CACAGCTAGA AGCCACTAAG CGTAAGCATC AGGAGATTCG TGCCATGCGC
    AGTCAGCTGA AGAAGATTGA GGATCTGGGA GCTGCCATGG AGGAGGCGCT GATTCTGGAC
    AATAAATACA CAGAACACAG CACCGTGGGT CTGGCACAAC AGTGGGACCA ACTCGACCAG
    CTGGGCATGA GAATGCAACA TAACCTGGAA CAACAGATCC AAGCCAGAAA CACTACAGGT
    GTAACAGAAG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTCGA CAAAGAAAAA
    TCTGGCCGTC TGAATCACCA GGAGTTCAAG TCTTGTCTGC GGTCTCTGGG TTACGACCTT
    CCCATGGTGG AAGAAGGAGA ACCAGACCCA GAGTTTGAGT CCATCCTGGA TATAGTGGAT
    CCAAACAGGG ATGGAAACGT GTCCTTGCAG GAGTACATGG CTTTCATGAT CAGCCGAGAA
    ACGGAGAACG TCAAGTCAAG CGAGGAGATC GAGAGCGCTT TCCGTGCTCT GAGCGCAGAA
    AACAAACCCT ACGTCACCAA GGAAGAACTC TACCAGAACC TGACAAAGGA ACAAGCCGAC
    TACTGCATTT CCCATATGAA GCCCTACCTA GACAGCAAGG GCCGCGAAAT CCCATCAGCT
    TTCGACTTTG TGGAATTCAC CCGCTCGCTC TTCGTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293479.1
    CDS114..7475
    Translation

    Target ORF information:

    RefSeq Version XM_009295204.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295204.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGACTG CGGCCACCAA GGAGGTGTGC
    AGCGTGTCTA TGCGCATGAA GCAGGTGGAG GAACTGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGG ATGAAGCAGA CTCCAAAGGT
    GCATCTCCAT GGAAGGAGCT GAACAACCGC TGGAGGTCTC TGCAGCAGCT GGCTGAAGAC
    CGAAGCAACA TGCTGGGCAG TGCTCACGAA GTTCAGAGGT TCCACAGAGA TGCAGATGAG
    ACCAAGGAGT GGATCGAAGA GAAGAACCAA GCCCTGAACA CTGATAACTA TGGCCATGAC
    CTCGCCAGCG TGCAGGCTCT GCAGCGCAAG CATGAAGGAT TTGAGAGAGA TCTAGCCGCT
    TTAGGAGACA AGGTGAATTC TCTTGGTGAG ACTGCAGAGC GTCTGATTCA ATCTCATCCT
    GAGGCTGTGG ATGACATCCA GGAGAAGTGC ACCGAGCTAA ACACAGCATG GAGCAGCCTG
    GTGGGTCGTG CAGACCAGCG CAAAGAAAAA CTGGGCAACT CCCATGACCT GCAGCGCTTC
    CTCAGTGACT TCAGAGATCT CATGTCTTGG ATCAATGGAA TCAGGGGACT TGTGTCTTCC
    GATGAGCTGG CCAAGGATGT TACTGGTGCT GAAGCCCTGT TGGAGAGACA TCAGGAACAC
    CGCACTGAGA TCGACGCTCG TGCAGGAACC TTCCTGGCTT TTGAGCAGTT TGGACAGCAG
    CTGCTCGCTC GTGGGCATTA CGCAAGCCCT GAGATCAAAC AGAAGCTGGA GGCTCTTGAC
    CGGGAGCGTG CTGACCTGGA GAAGGCCTGG GTTCAGCGCA GGATGATGCT GGACCAGTGC
    CTGGAGCTGC AGTTGTTTAA CCGTGACTGT GAGCAGGCTG AGAACTGGAT GGCTGCCCGC
    GAAGCCTTCT TAGCCAGCGA TGACAAGGGA GATTCTCTGG ACAGTGTGGA GGCGCTCATT
    AAGAAACACG AGGACTTTGA TAAGGCTATC AATGTGCAGG AAGAAAAGAT TGCTGCACTG
    CAGTCTTTTG CTGACCAGCT CATCAGCGCT GACCACTATG CCAAGCCTGA GATCTTCAAA
    CGACGCAGTG AGGTCTTGGA CAGGTGGCTC CGCCTCAAGG CTCAGATGAT TGAGAAACGC
    TCCAAGCTTG GCGAGTCACA GACTCTACAG CAGTTCAGCA GAGACGTTGA CGAGATCGAA
    GCTTGGATCA GTGAGAAACT CCAGACCGCC ACAGACGAGT CTTACAAAGA CCCCACCAAC
    ATCCAGCTGT CCAAGCTGCT GAGCAAACAC CAGAAACACC AGGCTTTTGA GGCTGAGCTA
    CACGCTAACG CTGATCGTAT CCGCGGAGTG ATTGACACGG GCAATGCTCT CATCCAGAGA
    GGTGCCTGTG CTGGAAGTGA AGATGCTGTT AAGGCTCGTC TGAATGCTCT GGACGAGCAG
    TGGCAGTTCC TGGTCAACAA GTCTGCTGAG AAGAGTCAGA AACTGAAGGA GGCCAACAAA
    CAGCAGAACT TCAACACTGG CATCAAGGAC TTTGACTTCT GGTTGTCTGA GGTTGAGGCT
    CTTCTTGCCT CTGAGGATTA TGGGAAAGAT CTGGCTTCAG TCAACAATCT GCTGAAGAAG
    CACCAGCTGT TGGAGGCTGA TATCTCTGCT CATGAGGATC GTTTGAAGGA TCTTAATGGC
    CAGGCTGACA GTCTGATGGC CAGCAACGCT TTCGACACAT CTCAGGTCAA AGACAAGCGG
    GATGCCGTTA ACGGACGCTT CGGCAAAATC AAGAGCATGG CAGCGGGACG GCGAGCCAAG
    CTGAACGAAT CGCATCGTCT GCACCAGTTC TTCAGGGATC TGGACGATGA GGAGTCCTGG
    ATTAAGGAAA AGAAGTTGTT GGTGAGCTCG GAGGACTACG GACGTGATTT GACAGGGGTG
    CAGAATCTGA GGAAGAAGCA CAAGAGGCTG GAGGCTGAGC TGGGAGCACA CGAGCCGGCC
    ATTCAGTCTG TGTTGGAGAC TGGGAAGAAG CTGTCTGACG ACAACACCAT TGGACAGGAG
    GAGATCCAGC AGAGACTGGC CCAGTTTGTG GAGCACTGGA GGGAGCTGAA GGATCTGGCT
    GCTGCACGTG GGCAGAGGCT TGAAGAGTCG CTGGAATACC AGCAGTTTGT GGCGAACGTG
    GAAGAAGAGG AAGCCTGGAT CAACGAGAAA CTGAACCTGG TCGGAAGTGA AGATTACGGT
    GATACTCTGG CAGCTGTGCA GGGCTTGCTG AAGAAACATG AAGCATTTGA AACTGATTTT
    ACTGTGCACA GGGACCGTGT GAATGATGTG TGTTCCAATG GAGATGAACT CGTTAAAAAG
    GAGAACCACA ATGTGGAGAA CATCATGGCT AAAATGAAGG CGTTGCGTGG AAAAGTGTTG
    GAGCTGGAGA GAGCAGCGGC ACAGAGGAAA GCCAAGCTGG ATGAAAACTC TGCCTTCCTG
    CAGTTCAACT GGAAGGCTGA TGTGGTGGAG TCCTGGATTG GTGAGAAGGA AAACAGCCTG
    AAGACTGATG ACTATGGTCG CGATCTCTCC TCTGTGCAGA CGCTGCTCAC CAAACAGGAA
    ACCTTTGATG CAGGACTTCA GGCCTTCCAG CAGGAGGGCA TCACCAACAT CACAGCTCTA
    AAAGACCAGC TGCTGGCAGC CAAACATGTG CAGTCTAAGG CCATCGAGGC TCGTCACGCC
    ACCCTGATGA AGCGCTGGAA CCAACTACTA TCCAACTCAG CAGCCCGCAA GAAGAAGCTT
    CTGGAAGCAC AGGACCACTT CAGAAAGGTG GAGGATCTTT TCCTCACGTT TGCCAAGAAA
    GCCTCAGCCT TCAACAGCTG GTTTGAGAAC GCAGAGGAAG ATCTGACCGA TCCTGTGAGG
    TGCAACTCTC TGGAGGAGAT TAGGGCACTG CGGGATGCCC ATGATGCCTT CCGCTCGTCC
    CTGAGCTCCG CAGAGGCCGA CTTCAGCCAG CTGGCAGAAC TCGATCGGCA GATTAAGAGC
    TACAACGTGG TGTCCAACCC ATACACCTGG TTCACCATGG AGGCTCTGGA GGAGACCTGG
    AGTAACCTTC AGAAGATCAT CAAGGAGCGA GAACTGGAGC TGCAGAAGGA GCAGCGCCGA
    CAGGAAGAGA ATGATAAACT CAGACAGGAG TTTGCTCAGC ATGCTAATGC TTTCCACCAA
    TGGCTGCAGG AGACCAGGAC ATACCTTCTG GATGGGTCCT GCATGGTGGA AGAATCTGGA
    ACTCTAGAGT CACAGCTAGA AGCCACTAAG CGTAAGCATC AGGAGATTCG TGCCATGCGC
    AGTCAGCTGA AGAAGATTGA GGATCTGGGA GCTGCCATGG AGGAGGCGCT GATTCTGGAC
    AATAAATACA CAGAACACAG CACCGTGGGT CTGGCACAAC AGTGGGACCA ACTCGACCAG
    CTGGGCATGA GAATGCAACA TAACCTGGAA CAACAGATCC AAGCCAGAAA CACTACAGGT
    GTAACAGAAG AGGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCACTTCGA CAAAGAAAAA
    TCTGGCCGTC TGAATCACCA GGAGTTCAAG TCTTGTCTGC GGTCTCTGGG TTACGACCTT
    CCCATGGTGG AAGAAGGAGA ACCAGACCCA GAGTTTGAGT CCATCCTGGA TATAGTGGAT
    CCAAACAGGG ATGGAAACGT GTCCTTGCAG GAGTACATGG CTTTCATGAT CAGCCGAGAA
    ACGGAGAACG TCAAGTCAAG CGAGGAGATC GAGAGCGCTT TCCGTGCTCT GAGCGCAGAA
    AACAAACCCT ACGTCACCAA GGAAGAACTC TACCAGAACC TGACAAAGGA ACAAGCCGAC
    TACTGCATTT CCCATATGAA GCCCTACCTA GACAGCAAGG GCCGCGAAAT CCCATCAGCT
    TTCGACTTTG TGGAATTCAC CCGCTCGCTC TTCGTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41939
    Clone ID Related Accession (Same CDS sequence) XM_009295205.2 , XM_021468834.1
    Accession Version XM_009295205.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GGTCCTGCAT GGTGGAAGAA
    TCTGGAACTC TAGAGTCACA GCTAGAAGCC ACTAAGCGTA AGCATCAGGA GATTCGTGCC
    ATGCGCAGTC AGCTGAAGAA GATTGAGGAT CTGGGAGCTG CCATGGAGGA GGCGCTGATT
    CTGGACAATA AATACACAGA ACACAGCACC GTGGGTCTGG CACAACAGTG GGACCAACTC
    GACCAGCTGG GCATGAGAAT GCAACATAAC CTGGAACAAC AGATCCAAGC CAGAAACACT
    ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG TTCAGCATGA TGTTCAAGCA CTTCGACAAA
    GAAAAATCTG GCCGTCTGAA TCACCAGGAG TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC
    GACCTTCCCA TGGTGGAAGA AGGAGAACCA GACCCAGAGT TTGAGTCCAT CCTGGATATA
    GTGGATCCAA ACAGGGATGG AAACGTGTCC TTGCAGGAGT ACATGGCTTT CATGATCAGC
    CGAGAAACGG AGAACGTCAA GTCAAGCGAG GAGATCGAGA GCGCTTTCCG TGCTCTGAGC
    GCAGAAAACA AACCCTACGT CACCAAGGAA GAACTCTACC AGAACCTGAC AAAGGAACAA
    GCCGACTACT GCATTTCCCA TATGAAGCCC TACCTAGACA GCAAGGGCCG CGAAATCCCA
    TCAGCTTTCG ACTTTGTGGA ATTCACCCGC TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293480.1
    CDS114..7481
    Translation

    Target ORF information:

    RefSeq Version XM_009295205.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295205.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GGTCCTGCAT GGTGGAAGAA
    TCTGGAACTC TAGAGTCACA GCTAGAAGCC ACTAAGCGTA AGCATCAGGA GATTCGTGCC
    ATGCGCAGTC AGCTGAAGAA GATTGAGGAT CTGGGAGCTG CCATGGAGGA GGCGCTGATT
    CTGGACAATA AATACACAGA ACACAGCACC GTGGGTCTGG CACAACAGTG GGACCAACTC
    GACCAGCTGG GCATGAGAAT GCAACATAAC CTGGAACAAC AGATCCAAGC CAGAAACACT
    ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG TTCAGCATGA TGTTCAAGCA CTTCGACAAA
    GAAAAATCTG GCCGTCTGAA TCACCAGGAG TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC
    GACCTTCCCA TGGTGGAAGA AGGAGAACCA GACCCAGAGT TTGAGTCCAT CCTGGATATA
    GTGGATCCAA ACAGGGATGG AAACGTGTCC TTGCAGGAGT ACATGGCTTT CATGATCAGC
    CGAGAAACGG AGAACGTCAA GTCAAGCGAG GAGATCGAGA GCGCTTTCCG TGCTCTGAGC
    GCAGAAAACA AACCCTACGT CACCAAGGAA GAACTCTACC AGAACCTGAC AAAGGAACAA
    GCCGACTACT GCATTTCCCA TATGAAGCCC TACCTAGACA GCAAGGGCCG CGAAATCCCA
    TCAGCTTTCG ACTTTGTGGA ATTCACCCGC TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41939
    Clone ID Related Accession (Same CDS sequence) XM_009295205.2 , XM_021468834.1
    Accession Version XM_021468834.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GGTCCTGCAT GGTGGAAGAA
    TCTGGAACTC TAGAGTCACA GCTAGAAGCC ACTAAGCGTA AGCATCAGGA GATTCGTGCC
    ATGCGCAGTC AGCTGAAGAA GATTGAGGAT CTGGGAGCTG CCATGGAGGA GGCGCTGATT
    CTGGACAATA AATACACAGA ACACAGCACC GTGGGTCTGG CACAACAGTG GGACCAACTC
    GACCAGCTGG GCATGAGAAT GCAACATAAC CTGGAACAAC AGATCCAAGC CAGAAACACT
    ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG TTCAGCATGA TGTTCAAGCA CTTCGACAAA
    GAAAAATCTG GCCGTCTGAA TCACCAGGAG TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC
    GACCTTCCCA TGGTGGAAGA AGGAGAACCA GACCCAGAGT TTGAGTCCAT CCTGGATATA
    GTGGATCCAA ACAGGGATGG AAACGTGTCC TTGCAGGAGT ACATGGCTTT CATGATCAGC
    CGAGAAACGG AGAACGTCAA GTCAAGCGAG GAGATCGAGA GCGCTTTCCG TGCTCTGAGC
    GCAGAAAACA AACCCTACGT CACCAAGGAA GAACTCTACC AGAACCTGAC AAAGGAACAA
    GCCGACTACT GCATTTCCCA TATGAAGCCC TACCTAGACA GCAAGGGCCG CGAAATCCCA
    TCAGCTTTCG ACTTTGTGGA ATTCACCCGC TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324509.1
    CDS4..7371
    Translation

    Target ORF information:

    RefSeq Version XM_021468834.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin alpha 2 (spna2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468834.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATATCA GTGGAGTCAA GGTTTTAGAG ACGGCAGATG ACATCCAAGA GCGCCGACAG 
    CAGGTTCTGG ACCGGTACCG TCGCTTCAAG GAGCTGTCTG TGGTGCGCAG ACAGAAACTC
    GAAGACTCTT ACCGGTTCCA GTTCTTCCGT CGGGATGCAG ATGAACTTGA GAAATGGATC
    CAAGAGAAGC TACAGATTGC CTCTGATGAG AACTACAAGG ACCCCAGTAA CCTGCAGGGA
    AAGCTCCAGA AACATCAAGC GTTTGAAGCA GAGGTGCAGG CTAACGCCGA GGCCATCATC
    AAACTGGACG AGACCGGCAA CCTCATGATA TCCGAGGGCC ACTTTGCATC TGAAACCATC
    CGCACCCGTC TAGAGGAGCT TCACCGTCTG TGGGACTTGC TGCTGCAAAA GACTAAAGAG
    AAGGGAATCC GTCTCCTGCA GGCCCAAAAA CTGGTTCAGT ACCTCCGTGA GTGTGAGGAT
    GCCCTAGACT GGATCAGTGA CAAGGAAGCT ATTGTCACCT CAGAGGAGCT TGGGCAGGAC
    CTGGAACATG TGGAGGTCTT GCAGAAGAAG TTTGAAGAGT TCCAGACCGA TCTGGCAGCC
    CATGAAGAGC GTGTGAATGA GGTGAACCAG GCAGCCGGCA AACTAACCCA GGAGAACCAC
    CCAGAAGCTG AGCTCATCCT GAAAAAGCAG GAGGAGGTGA ACTCCGCCTG GCAGAGGCTG
    AAGGGCTTGG CTCAGCAGAG GCAGGGCAAG CTGTTTGGAG CTGCTGAAGT GCAGCGTTTC
    AACCGGGATG TGGATGAGAC AATCAGCTGG ATTAAGGAGA AGGAGCAGTT GATGGCATCT
    GATGATTTTG GTCGTGACCT CGCCAGCGTT CAAGCTTTGC TCCGGAAACA CGAAGGGCTG
    GAAAGAGATT TGGCAGCTTT GAATGACAAG GTAAACACTC TTGGTGGCGA GGCGGACCGT
    CTGCAGCAAA CACACCCCCA GAATGCCACT CAAATCCACC TGAAGAGAGA TGAACTCATT
    ACCAACTGGG AGCAGATCCA AACCCTGGCG ACTGAGCGAC ATGCTCGACT TAATGACTCC
    TACATGCTGC AGCGCTTCAC TGCAGATTTC CGCGACTTGA CGAGCTGGGT GACCGAGATG
    AAGGCTTTGA TCAATGCAGA TGAACTGGCC AACGATGTGG CTGGAGCGGA GGCCCTGCTT
    GATCGCCACC AGGAGCACAA GGGTGAGATC GACGCTCATG AGGACAGTTT CAAATCTACT
    GATGAGGCCG GTCAGGCACT GCTTAACACT GGACATTATG CTTCTGAGGA AGTCAAAGAA
    AAACTGGGCA TCTTGTCTGA GGAGAAAGTG TCTCTTCTGG AGCTGTGGGA GCTCCGCAGG
    CAGCAATACG AGCAGTGCAT GGACCTGCAG CTCTTTTACA GGGACACTGA GCAGGTTGAC
    AACTGGATGA GCAAGCAGGA GGCTTTCCTG CTGAATGAAG ATCTGGGCGA TTCTCTGGAC
    AGTGTTGAGG CTCTTCTAAA GAAACACGAA GACTTTGAGA AATCCCTCAG TGCTCAGGAA
    GAAAAGATCA CTGCGCTGGA TGAATTTGCC ACCAAACTGA TCCAGAACAA TCACTACGCC
    AAGGATGATG TGGCCACCCG CAGAGATGCA CTGCTGAGCA GACGCAACGC TCTTCATGAG
    CGGGCTCAGT TTCGCCGCGC TGCCCTGGAG GACTCCTTCC ATCTGCAGCA GTTCTTCCGA
    GACTCTGATG AGCTCAAGAG CTGGATTAAC GAGAAGATGA AGACCGCCAC TGATGAGGCC
    TACAAGGATC CATCCAACCT GCAGGGAAAG GTCCAGAAGC ACCAGGCCTT CGAGGCTGAG
    CTTTCTGCCA ATCAGAGCCG CATTGATGCA CTGCAGAAGT CAGGCCAGGA GCTGGTGGAT
    GGGAAACATT ATGCCTCCAG CGAGGTGTCC ACCCGAATGG ATGAAGTCAG CTCTCAATGG
    AAGAAGCTTC TGGAGGCAAC TGAGCTCAAA GGGATTAAGT TGCGTGAAGC GAACCAGCAG
    CAGCAGTTCA ATCGCAATGT GGAGGACATC GAGCTTTGGC TGTATGAGGT GGAGGGACAT
    CTGGCCTCTG ATGACTTTGG CAAAGACCTG ACCAGCGTCC AGAATCTCCA GAAGAAACAT
    GCTCTTCTAG AAGCTGACGT GGCCGCTCAC CAGGACCGTA TTGATGGCAT TACCATCCAG
    GCCAGGCAGT TCCAAGATGC CGGCCATTTT GATGCTGATA ACATCCGGAA AAAACAAGAG
    GCTCTAGTGG CACGTTATGA GGCCCTGAAG GAGCCCATGG CTGCCCGCAA ACAGAAGCTG
    TCCGACTCTC TGCGCCTTCA GCAACTTTTT AGGGATGTAG AAGATGAGGA GACCTGGATC
    AGAGAGAAGG AACCCATTGC TTCCTCCACC AACAGAGGAA AAGACCTTAT TGGGGTCCAA
    AACCTTCTGA AGAAACACCA GGCTCTGCAG GCAGAAATCT CTGGCCACGA ACCCCGAATC
    AAGGCTGTTA CTCAGAAGGG GGAGGCCATG ATTGATGAAG GTCACTTTGC AGGGGAGGAT
    GTGAAGGCTA AACTACAAGA GCTGCACAAC CGCTGGGACG GGCTGAAAGG AAAAGCAGCC
    CAGCGTAGAC AGGATCTGGA GGACTCTCTC CAGGCTCAGC AGTACTTTGC TGATGCCAAC
    GAGGCCGAGT CCTGGATGAG GGAGAAGGAG CCCATTGTAG GAAGCCCTGA CTACGGCAAA
    GATGAGGACT CTGCTGAGGC TTTGCTGAAG AAGCATGAGG CTCTGATGTC TGATTTGAGT
    GCATATGGCA GCAGCATCCA GGCCTTGAAA GAAGAAGCTC AGGCCTGCAG ACAACAAGTG
    GCCCCCACTG ATGACGAGAC TGGCAAGGAG CTGGTTCTGG CTCTGTACGA CTACCAGGAG
    AAGAGTCCAC GTGAGGTCAC CATGAAGAAG GGAGACATCC TCACTCTGCT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACAGGCAGG GCTTTGTTCC CGCAGCGTAC
    GTGAAAAAGT TGGACCCGAC TCAATCTTCC TCTCGTGAAA ATCTGCTGGA CGAACAGGGC
    AGCATCGGTT TGCGACAGGA TCAGATTGAG AACCAGTATG GTACACTGCT AGAGTTGGGA
    GAGAAGAGGA AGGACATGCT GGAGAAGAGC TGCAAGAAGT TCATGCTTTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAATGAGAAA GAGTCTGCGC TTACTAACGA GGAGGTGGGA
    TCTGACCTGG AGCAGGTGGA AGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCAAATG AGTCTCGGCT GAGGGACATA AACAAGGTGG CGTCTGAGCT GGAGTCTGAA
    GGTCTGATGG CAGAAGAGGC TCCTGTGGTT CAGGCCCAGC AAGTAGAGGT GCTGGGCTCA
    GCTCCTGGCA AGGATGAAGC AGACTCCAAA GGTGCATCTC CATGGAAGTC TGTTCGCTTG
    GCTGTTCAAA CAACGGCCAA CTTTAATACT ATCAAGGAGC TGAACAACCG CTGGAGGTCT
    CTGCAGCAGC TGGCTGAAGA CCGAAGCAAC ATGCTGGGCA GTGCTCACGA AGTTCAGAGG
    TTCCACAGAG ATGCAGATGA GACCAAGGAG TGGATCGAAG AGAAGAACCA AGCCCTGAAC
    ACTGATAACT ATGGCCATGA CCTCGCCAGC GTGCAGGCTC TGCAGCGCAA GCATGAAGGA
    TTTGAGAGAG ATCTAGCCGC TTTAGGAGAC AAGGTGAATT CTCTTGGTGA GACTGCAGAG
    CGTCTGATTC AATCTCATCC TGAGGCTGTG GATGACATCC AGGAGAAGTG CACCGAGCTA
    AACACAGCAT GGAGCAGCCT GGTGGGTCGT GCAGACCAGC GCAAAGAAAA ACTGGGCAAC
    TCCCATGACC TGCAGCGCTT CCTCAGTGAC TTCAGAGATC TCATGTCTTG GATCAATGGA
    ATCAGGGGAC TTGTGTCTTC CGATGAGCTG GCCAAGGATG TTACTGGTGC TGAAGCCCTG
    TTGGAGAGAC ATCAGGAACA CCGCACTGAG ATCGACGCTC GTGCAGGAAC CTTCCTGGCT
    TTTGAGCAGT TTGGACAGCA GCTGCTCGCT CGTGGGCATT ACGCAAGCCC TGAGATCAAA
    CAGAAGCTGG AGGCTCTTGA CCGGGAGCGT GCTGACCTGG AGAAGGCCTG GGTTCAGCGC
    AGGATGATGC TGGACCAGTG CCTGGAGCTG CAGTTGTTTA ACCGTGACTG TGAGCAGGCT
    GAGAACTGGA TGGCTGCCCG CGAAGCCTTC TTAGCCAGCG ATGACAAGGG AGATTCTCTG
    GACAGTGTGG AGGCGCTCAT TAAGAAACAC GAGGACTTTG ATAAGGCTAT CAATGTGCAG
    GAAGAAAAGA TTGCTGCACT GCAGTCTTTT GCTGACCAGC TCATCAGCGC TGACCACTAT
    GCCAAGCCTG AGATCTTCAA ACGACGCAGT GAGGTCTTGG ACAGGTGGCT CCGCCTCAAG
    GCTCAGATGA TTGAGAAACG CTCCAAGCTT GGCGAGTCAC AGACTCTACA GCAGTTCAGC
    AGAGACGTTG ACGAGATCGA AGCTTGGATC AGTGAGAAAC TCCAGACCGC CACAGACGAG
    TCTTACAAAG ACCCCACCAA CATCCAGAGC AAACACCAGA AACACCAGGC TTTTGAGGCT
    GAGCTACACG CTAACGCTGA TCGTATCCGC GGAGTGATTG ACACGGGCAA TGCTCTCATC
    CAGAGAGGTG CCTGTGCTGG AAGTGAAGAT GCTGTTAAGG CTCGTCTGAA TGCTCTGGAC
    GAGCAGTGGC AGTTCCTGGT CAACAAGTCT GCTGAGAAGA GTCAGAAACT GAAGGAGGCC
    AACAAACAGC AGAACTTCAA CACTGGCATC AAGGACTTTG ACTTCTGGTT GTCTGAGGTT
    GAGGCTCTTC TTGCCTCTGA GGATTATGGG AAAGATCTGG CTTCAGTCAA CAATCTGCTG
    AAGAAGCACC AGCTGTTGGA GGCTGATATC TCTGCTCATG AGGATCGTTT GAAGGATCTT
    AATGGCCAGG CTGACAGTCT GATGGCCAGC AACGCTTTCG ACACATCTCA GGTCAAAGAC
    AAGCGGGATG CCGTTAACGG ACGCTTCGGC AAAATCAAGA GCATGGCAGC GGGACGGCGA
    GCCAAGCTGA ACGAATCGCA TCGTCTGCAC CAGTTCTTCA GGGATCTGGA CGATGAGGAG
    TCCTGGATTA AGGAAAAGAA GTTGTTGGTG AGCTCGGAGG ACTACGGACG TGATTTGACA
    GGGGTGCAGA ATCTGAGGAA GAAGCACAAG AGGCTGGAGG CTGAGCTGGG AGCACACGAG
    CCGGCCATTC AGTCTGTGTT GGAGACTGGG AAGAAGCTGT CTGACGACAA CACCATTGGA
    CAGGAGGAGA TCCAGCAGAG ACTGGCCCAG TTTGTGGAGC ACTGGAGGGA GCTGAAGGAT
    CTGGCTGCTG CACGTGGGCA GAGGCTTGAA GAGTCGCTGG AATACCAGCA GTTTGTGGCG
    AACGTGGAAG AAGAGGAAGC CTGGATCAAC GAGAAACTGA ACCTGGTCGG AAGTGAAGAT
    TACGGTGATA CTCTGGCAGC TGTGCAGGGC TTGCTGAAGA AACATGAAGC ATTTGAAACT
    GATTTTACTG TGCACAGGGA CCGTGTGAAT GATGTGTGTT CCAATGGAGA TGAACTCGTT
    AAAAAGGAGA ACCACAATGT GGAGAACATC ATGGCTAAAA TGAAGGCGTT GCGTGGAAAA
    GTGTTGGAGC TGGAGAGAGC AGCGGCACAG AGGAAAGCCA AGCTGGATGA AAACTCTGCC
    TTCCTGCAGT TCAACTGGAA GGCTGATGTG GTGGAGTCCT GGATTGGTGA GAAGGAAAAC
    AGCCTGAAGA CTGATGACTA TGGTCGCGAT CTCTCCTCTG TGCAGACGCT GCTCACCAAA
    CAGGAAACCT TTGATGCAGG ACTTCAGGCC TTCCAGCAGG AGGGCATCAC CAACATCACA
    GCTCTAAAAG ACCAGCTGCT GGCAGCCAAA CATGTGCAGT CTAAGGCCAT CGAGGCTCGT
    CACGCCACCC TGATGAAGCG CTGGAACCAA CTACTATCCA ACTCAGCAGC CCGCAAGAAG
    AAGCTTCTGG AAGCACAGGA CCACTTCAGA AAGGTGGAGG ATCTTTTCCT CACGTTTGCC
    AAGAAAGCCT CAGCCTTCAA CAGCTGGTTT GAGAACGCAG AGGAAGATCT GACCGATCCT
    GTGAGGTGCA ACTCTCTGGA GGAGATTAGG GCACTGCGGG ATGCCCATGA TGCCTTCCGC
    TCGTCCCTGA GCTCCGCAGA GGCCGACTTC AGCCAGCTGG CAGAACTCGA TCGGCAGATT
    AAGAGCTACA ACGTGGTGTC CAACCCATAC ACCTGGTTCA CCATGGAGGC TCTGGAGGAG
    ACCTGGAGTA ACCTTCAGAA GATCATCAAG GAGCGAGAAC TGGAGCTGCA GAAGGAGCAG
    CGCCGACAGG AAGAGAATGA TAAACTCAGA CAGGAGTTTG CTCAGCATGC TAATGCTTTC
    CACCAATGGC TGCAGGAGAC CAGGACATAC CTTCTGGATG GGTCCTGCAT GGTGGAAGAA
    TCTGGAACTC TAGAGTCACA GCTAGAAGCC ACTAAGCGTA AGCATCAGGA GATTCGTGCC
    ATGCGCAGTC AGCTGAAGAA GATTGAGGAT CTGGGAGCTG CCATGGAGGA GGCGCTGATT
    CTGGACAATA AATACACAGA ACACAGCACC GTGGGTCTGG CACAACAGTG GGACCAACTC
    GACCAGCTGG GCATGAGAAT GCAACATAAC CTGGAACAAC AGATCCAAGC CAGAAACACT
    ACAGGTGTAA CAGAAGAGGC CCTGAAGGAG TTCAGCATGA TGTTCAAGCA CTTCGACAAA
    GAAAAATCTG GCCGTCTGAA TCACCAGGAG TTCAAGTCTT GTCTGCGGTC TCTGGGTTAC
    GACCTTCCCA TGGTGGAAGA AGGAGAACCA GACCCAGAGT TTGAGTCCAT CCTGGATATA
    GTGGATCCAA ACAGGGATGG AAACGTGTCC TTGCAGGAGT ACATGGCTTT CATGATCAGC
    CGAGAAACGG AGAACGTCAA GTCAAGCGAG GAGATCGAGA GCGCTTTCCG TGCTCTGAGC
    GCAGAAAACA AACCCTACGT CACCAAGGAA GAACTCTACC AGAACCTGAC AAAGGAACAA
    GCCGACTACT GCATTTCCCA TATGAAGCCC TACCTAGACA GCAAGGGCCG CGAAATCCCA
    TCAGCTTTCG ACTTTGTGGA ATTCACCCGC TCGCTCTTCG TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.