ZGC:162193 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZGC:162193
Entrez Gene ID	323315
Full Name	zgc:162193
Synonyms	fam115,fb95a05,tcaf,wu:fb95a05
General protein information	Preferred Names zgc:162193 Names TRPM8 channel-associated factor homolog protein FAM115
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291538.2	XP_009289813.1	TRPM8 channel-associated factor homolog isoform X1
XM_017359215.1	XP_017214704.1	TRPM8 channel-associated factor homolog isoform X1
XM_009291538.3	XP_009289813.1	TRPM8 channel-associated factor homolog isoform X1
XM_021481341.1	XP_021337016.1	TRPM8 channel-associated factor homolog isoform X1
NM_001089327.1	NP_001082796.1	TRPM8 channel-associated factor homolog
XM_009291537.2	XP_009289812.1	TRPM8 channel-associated factor homolog isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FAM115C	NP_775949.2
Canis lupus familiaris (dog)	FAM115C	XP_539847.2
Xenopus tropicalis (tropical clawed frog)	LOC100485132	XP_002941113.2
Xenopus tropicalis (tropical clawed frog)	LOC100498358	XP_002934625.2
Mus musculus (house mouse)	Fam115c	NP_666286.1
Danio rerio (zebrafish)	LOC100535998	XP_003200030.1
Xenopus tropicalis (tropical clawed frog)	LOC100489234	XP_002943401.2
Pan troglodytes (chimpanzee)	FAM115C	XP_519453.3
	FAM115C	XP_001092570.2
Bos taurus (cattle)	FAM115C	NP_001095394.1
Xenopus tropicalis (tropical clawed frog)	fam115c	XP_002934627.2
Danio rerio (zebrafish)	LOC100535908	XP_003200029.1
Danio rerio (zebrafish)	zgc:162193	NP_001082796.1
Danio rerio (zebrafish)	LOC555689	XP_683371.5
Xenopus tropicalis (tropical clawed frog)	fam115a	XP_002934606.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.
Gkika D, Lemonnier L, Shapovalov G, Gordienko D, Poux C, Bernardini M, Bokhobza A, Bidaux G, Degerny C, Verreman K, Guarmit B, Benahmed M, de Launoit Y, Bindels RJ, Fiorio Pla A, Prevarskaya N
The Journal of cell biology208(1)89-107(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ZGC:162193 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZGC:162193 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38349	NM_001089327.1 Latest version!	Danio rerio zgc:162193 (zgc:162193), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa20272	XM_009291537.2 Latest version!	Danio rerio zgc:162193 (zgc:162193), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa20272	XM_009291538.3 Latest version!	Danio rerio zgc:162193 (zgc:162193), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa20272	XM_021481341.1 Latest version!	Danio rerio zgc:162193 (zgc:162193), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa20272	XM_017359215.1 Latest version!	Danio rerio zgc:162193 (zgc:162193), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa20272	XM_009291538.2	Danio rerio zgc:162193 (zgc:162193), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZGC:162193 ( NM_001089327.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001082796.1 Danio rerio zgc:162193 (zgc:162193), mRNA.

Price & Availability↑

CloneID	ODa38349
Clone ID Related Accession (Same CDS sequence)	NM_001089327.1
Accession Version	NM_001089327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	TRPM8 channel-associated factor homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134919.1. ##Evidence-Data-START## Transcript exon combination :: BC134919.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAAAGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC AGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGTGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGTGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGCAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GGGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTATGT 
AGTAACTGCC CTGTAGAAAG TCCCAAAGAT CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGATGCCCTC CTTCCTTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTCCAAGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGAAT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACGAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCTCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TTATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGC TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GATATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082796.1
CDS	169..2907
Translation	MAREQDYYTLMSGMQQLDLQGKAVPSDLVLIGEHAFPLAMNPRGQVLMAASHYGRGRIVVLGHEEYLTRFPGLIENAIMWLMPCTGDAGVVGIQKSLRTVAENLNYSPFKTELGDFQDRFAIYITDAYSVESSAKDLIAFLKAGGGLIIAGQAWSWSYEHPQENTIRNFPGNKVCSVAGIYFSELEGEVGTFPVPRNIPSSWLAVAIGKDFKEDLEFLLEGVSEFDTQGGAIASEVMVHGPLAFPIAVTPNGKTFIAGAYYGQGRVIVVSHEGYMGRDSLSSFMINAIKWLDEGRKGVVGIVPNLTAAHTVLSKSGLDCQLTGFREDLSVYVCTSYSDAQCAEIQEFVAEGGGLLIGGHAWYWAQTHCGSNVMTEYPGNHILNKMGLCLLGNTLGGGLYKAPEIEHSCKHGYHFRSMLQRFAQHVSQGQELTDREQGCLKQLGTDCASYLQMRCHDSAAYTSVVALLSDIVKEVGVPQVCSNCPVESPKDHLMLHIGTEVYKVTPDPDALLPYIIKDRPNLPTLSNARVRITANTGGCEEWISTGLYLSPGMKTYIAVPPEIVGKNWQVQLGCQTDNIGGSNTLKRAPVVHARFPLDSEMVQVWNLWGGLIYLIAPSQTKVDGVEIVVQNAVQAPYFKSGETSVADWVSHIRQAPAPWAELEFENLIMTFDSAFIRNLDRPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPIMMHSGSAPGLVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCNLWSLYVHEQVFGIKSANAHPAITPADRQARTKMYFDGGKDLNSWCMWMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPNDNAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLPAWSDHPVSQYA

Target ORF information:

RefSeq Version	NM_001089327.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162193 (zgc:162193), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089327.1

ORF Insert Sequence:

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAAAGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC AGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGTGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGTGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGCAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GGGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTATGT 
AGTAACTGCC CTGTAGAAAG TCCCAAAGAT CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGATGCCCTC CTTCCTTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTCCAAGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGAAT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACGAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCTCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TTATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGC TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GATATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGC:162193 ( XM_009291538.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289813.1 Danio rerio zgc:162193 (zgc:162193), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa20272
Clone ID Related Accession (Same CDS sequence)	XM_009291538.2 , XM_017359215.1 , XM_009291538.3 , XM_021481341.1 , XM_009291537.2
Accession Version	XM_009291538.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRPM8 channel-associated factor homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289813.1
CDS	244..2982
Translation	MAREQDYYTLMSGMQQLDLQGNAVPSDLVLIGEHAFPLAMNPRGQVLMAASHYGRGRIVVLGHEEYLTRFPGLIENAIMWLMPCTGDAGVVGIQKSLRTVAENLNYSPFKTELGDFQDRFAIYITDAYSVESSAKDLIAFLKAGGGLIIAGQAWSWSYEHPQENTIRNFPGNKVCSVAGIYFSELEGEVGTFPVPRNIPSSWLAVAIGKDFKEDLEFLLEGVSEFDTQGGAIASEVMVHGPLAFPIAVTPNGKTFIAGAYYGQGRVIVVSHEGYMGRDSLSSFMINAIKWLDEGRKGVVGIVPNLTAAHTVLSKSGLDCQLTGFREDLSVYVCTSYSDAQCAEIQEFVAEGGGLLIGGHAWYWAQTHWGSNVMTEYPGNHILNKMGLCLLGNTLGGGLYKAPEIEHSCKHGYHFRSMLQRFAQHVSQGQELTDREQGCLKQLGTDCASYLQMRCHDSAAYTSVVALLSDIVKEVGVPQVCSNCPVESPKDHLMLHIGTEVYKVTPDPDALLPYIIKDRPNLPTLSNARVRITANTGGCEEWISTGLYLSPGMKTYIAVPPEIVGKNWQVQLGCQTDNIGGSNTLKRAPVVHARFPLDSEMVQVWNLWGGLIYLIAPSQTKVDGVEIVVQTAVQAPYFKSGETSVADWVSHIRQAPAPWAELEFENLIMTFDSAFIRNLDRPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPIMMHSGSAPGLVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCNLWSLYVHEQVFGIKSANAHPAITPADRQARTKMYFDGGKDLNSWCMWMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPNDNAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLPAWSDHPVSQYA

Target ORF information:

RefSeq Version	XM_009291538.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:162193 (zgc:162193), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291538.2

ORF Insert Sequence:

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGC:162193 ( XM_017359215.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214704.1 Danio rerio zgc:162193 (zgc:162193), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20272
Clone ID Related Accession (Same CDS sequence)	XM_009291538.2 , XM_017359215.1 , XM_009291538.3 , XM_021481341.1 , XM_009291537.2
Accession Version	XM_017359215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRPM8 channel-associated factor homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214704.1
CDS	2679..5417
Translation	MAREQDYYTLMSGMQQLDLQGNAVPSDLVLIGEHAFPLAMNPRGQVLMAASHYGRGRIVVLGHEEYLTRFPGLIENAIMWLMPCTGDAGVVGIQKSLRTVAENLNYSPFKTELGDFQDRFAIYITDAYSVESSAKDLIAFLKAGGGLIIAGQAWSWSYEHPQENTIRNFPGNKVCSVAGIYFSELEGEVGTFPVPRNIPSSWLAVAIGKDFKEDLEFLLEGVSEFDTQGGAIASEVMVHGPLAFPIAVTPNGKTFIAGAYYGQGRVIVVSHEGYMGRDSLSSFMINAIKWLDEGRKGVVGIVPNLTAAHTVLSKSGLDCQLTGFREDLSVYVCTSYSDAQCAEIQEFVAEGGGLLIGGHAWYWAQTHWGSNVMTEYPGNHILNKMGLCLLGNTLGGGLYKAPEIEHSCKHGYHFRSMLQRFAQHVSQGQELTDREQGCLKQLGTDCASYLQMRCHDSAAYTSVVALLSDIVKEVGVPQVCSNCPVESPKDHLMLHIGTEVYKVTPDPDALLPYIIKDRPNLPTLSNARVRITANTGGCEEWISTGLYLSPGMKTYIAVPPEIVGKNWQVQLGCQTDNIGGSNTLKRAPVVHARFPLDSEMVQVWNLWGGLIYLIAPSQTKVDGVEIVVQTAVQAPYFKSGETSVADWVSHIRQAPAPWAELEFENLIMTFDSAFIRNLDRPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPIMMHSGSAPGLVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCNLWSLYVHEQVFGIKSANAHPAITPADRQARTKMYFDGGKDLNSWCMWMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPNDNAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLPAWSDHPVSQYA

Target ORF information:

RefSeq Version	XM_017359215.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:162193 (zgc:162193), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359215.1

ORF Insert Sequence:

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGC:162193 ( XM_009291538.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289813.1 Danio rerio zgc:162193 (zgc:162193), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20272
Clone ID Related Accession (Same CDS sequence)	XM_009291538.2 , XM_017359215.1 , XM_009291538.3 , XM_021481341.1 , XM_009291537.2
Accession Version	XM_009291538.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TRPM8 channel-associated factor homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289813.1
CDS	255..2993
Translation	MAREQDYYTLMSGMQQLDLQGNAVPSDLVLIGEHAFPLAMNPRGQVLMAASHYGRGRIVVLGHEEYLTRFPGLIENAIMWLMPCTGDAGVVGIQKSLRTVAENLNYSPFKTELGDFQDRFAIYITDAYSVESSAKDLIAFLKAGGGLIIAGQAWSWSYEHPQENTIRNFPGNKVCSVAGIYFSELEGEVGTFPVPRNIPSSWLAVAIGKDFKEDLEFLLEGVSEFDTQGGAIASEVMVHGPLAFPIAVTPNGKTFIAGAYYGQGRVIVVSHEGYMGRDSLSSFMINAIKWLDEGRKGVVGIVPNLTAAHTVLSKSGLDCQLTGFREDLSVYVCTSYSDAQCAEIQEFVAEGGGLLIGGHAWYWAQTHWGSNVMTEYPGNHILNKMGLCLLGNTLGGGLYKAPEIEHSCKHGYHFRSMLQRFAQHVSQGQELTDREQGCLKQLGTDCASYLQMRCHDSAAYTSVVALLSDIVKEVGVPQVCSNCPVESPKDHLMLHIGTEVYKVTPDPDALLPYIIKDRPNLPTLSNARVRITANTGGCEEWISTGLYLSPGMKTYIAVPPEIVGKNWQVQLGCQTDNIGGSNTLKRAPVVHARFPLDSEMVQVWNLWGGLIYLIAPSQTKVDGVEIVVQTAVQAPYFKSGETSVADWVSHIRQAPAPWAELEFENLIMTFDSAFIRNLDRPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPIMMHSGSAPGLVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCNLWSLYVHEQVFGIKSANAHPAITPADRQARTKMYFDGGKDLNSWCMWMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPNDNAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLPAWSDHPVSQYA

Target ORF information:

RefSeq Version	XM_009291538.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162193 (zgc:162193), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291538.3

ORF Insert Sequence:

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGC:162193 ( XM_021481341.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021337016.1 Danio rerio zgc:162193 (zgc:162193), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20272
Clone ID Related Accession (Same CDS sequence)	XM_009291538.2 , XM_017359215.1 , XM_009291538.3 , XM_021481341.1 , XM_009291537.2
Accession Version	XM_021481341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TRPM8 channel-associated factor homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337016.1
CDS	235..2973
Translation	MAREQDYYTLMSGMQQLDLQGNAVPSDLVLIGEHAFPLAMNPRGQVLMAASHYGRGRIVVLGHEEYLTRFPGLIENAIMWLMPCTGDAGVVGIQKSLRTVAENLNYSPFKTELGDFQDRFAIYITDAYSVESSAKDLIAFLKAGGGLIIAGQAWSWSYEHPQENTIRNFPGNKVCSVAGIYFSELEGEVGTFPVPRNIPSSWLAVAIGKDFKEDLEFLLEGVSEFDTQGGAIASEVMVHGPLAFPIAVTPNGKTFIAGAYYGQGRVIVVSHEGYMGRDSLSSFMINAIKWLDEGRKGVVGIVPNLTAAHTVLSKSGLDCQLTGFREDLSVYVCTSYSDAQCAEIQEFVAEGGGLLIGGHAWYWAQTHWGSNVMTEYPGNHILNKMGLCLLGNTLGGGLYKAPEIEHSCKHGYHFRSMLQRFAQHVSQGQELTDREQGCLKQLGTDCASYLQMRCHDSAAYTSVVALLSDIVKEVGVPQVCSNCPVESPKDHLMLHIGTEVYKVTPDPDALLPYIIKDRPNLPTLSNARVRITANTGGCEEWISTGLYLSPGMKTYIAVPPEIVGKNWQVQLGCQTDNIGGSNTLKRAPVVHARFPLDSEMVQVWNLWGGLIYLIAPSQTKVDGVEIVVQTAVQAPYFKSGETSVADWVSHIRQAPAPWAELEFENLIMTFDSAFIRNLDRPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPIMMHSGSAPGLVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCNLWSLYVHEQVFGIKSANAHPAITPADRQARTKMYFDGGKDLNSWCMWMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPNDNAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLPAWSDHPVSQYA

Target ORF information:

RefSeq Version	XM_021481341.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162193 (zgc:162193), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481341.1

ORF Insert Sequence:

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGC:162193 ( XM_009291537.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289812.1 Danio rerio zgc:162193 (zgc:162193), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20272
Clone ID Related Accession (Same CDS sequence)	XM_009291538.2 , XM_017359215.1 , XM_009291538.3 , XM_021481341.1 , XM_009291537.2
Accession Version	XM_009291537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TRPM8 channel-associated factor homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289812.1
CDS	334..3072
Translation	MAREQDYYTLMSGMQQLDLQGNAVPSDLVLIGEHAFPLAMNPRGQVLMAASHYGRGRIVVLGHEEYLTRFPGLIENAIMWLMPCTGDAGVVGIQKSLRTVAENLNYSPFKTELGDFQDRFAIYITDAYSVESSAKDLIAFLKAGGGLIIAGQAWSWSYEHPQENTIRNFPGNKVCSVAGIYFSELEGEVGTFPVPRNIPSSWLAVAIGKDFKEDLEFLLEGVSEFDTQGGAIASEVMVHGPLAFPIAVTPNGKTFIAGAYYGQGRVIVVSHEGYMGRDSLSSFMINAIKWLDEGRKGVVGIVPNLTAAHTVLSKSGLDCQLTGFREDLSVYVCTSYSDAQCAEIQEFVAEGGGLLIGGHAWYWAQTHWGSNVMTEYPGNHILNKMGLCLLGNTLGGGLYKAPEIEHSCKHGYHFRSMLQRFAQHVSQGQELTDREQGCLKQLGTDCASYLQMRCHDSAAYTSVVALLSDIVKEVGVPQVCSNCPVESPKDHLMLHIGTEVYKVTPDPDALLPYIIKDRPNLPTLSNARVRITANTGGCEEWISTGLYLSPGMKTYIAVPPEIVGKNWQVQLGCQTDNIGGSNTLKRAPVVHARFPLDSEMVQVWNLWGGLIYLIAPSQTKVDGVEIVVQTAVQAPYFKSGETSVADWVSHIRQAPAPWAELEFENLIMTFDSAFIRNLDRPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPIMMHSGSAPGLVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCNLWSLYVHEQVFGIKSANAHPAITPADRQARTKMYFDGGKDLNSWCMWMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPNDNAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLPAWSDHPVSQYA

Target ORF information:

RefSeq Version	XM_009291537.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:162193 (zgc:162193), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291537.2

ORF Insert Sequence:

ATGGCACGTG AACAGGACTA CTACACTCTC ATGTCTGGTA TGCAACAGCT TGATTTGCAG 
GGGAACGCAG TCCCCAGTGA TCTTGTACTA ATAGGAGAGC ATGCCTTTCC ACTTGCCATG 
AATCCAAGAG GTCAGGTGCT CATGGCTGCA TCTCATTACG GTCGGGGTCG CATTGTAGTG 
CTGGGACATG AAGAATACTT AACACGATTT CCTGGGCTGA TAGAGAATGC CATAATGTGG 
CTCATGCCAT GTACCGGAGA TGCTGGAGTT GTAGGGATTC AGAAAAGTTT ACGAACAGTA 
GCTGAAAACT TGAACTACAG CCCTTTCAAG ACAGAACTAG GAGACTTTCA AGATAGGTTC 
GCTATATATA TCACTGATGC GTACAGTGTT GAGAGCTCTG CAAAGGATCT GATTGCTTTC 
CTCAAAGCTG GGGGTGGTTT GATCATTGCT GGACAAGCAT GGAGTTGGTC TTATGAACAC 
CCACAGGAAA ACACTATCAG GAACTTTCCA GGAAACAAGG TGTGCAGTGT TGCAGGCATT 
TACTTTTCAG AGCTTGAAGG AGAAGTCGGC ACCTTTCCTG TTCCTCGAAA TATCCCTTCT 
AGTTGGCTTG CCGTGGCAAT AGGCAAAGAC TTTAAAGAGG ACCTAGAGTT CCTGCTGGAA 
GGTGTGTCTG AGTTTGACAC TCAAGGTGGG GCTATAGCAT CAGAGGTGAT GGTGCACGGA 
CCATTAGCGT TTCCCATTGC GGTCACTCCA AATGGAAAAA CATTCATTGC TGGTGCCTAT 
TATGGACAAG GGCGAGTAAT TGTGGTATCC CATGAAGGGT ACATGGGCCG AGACTCTCTG 
TCCTCTTTTA TGATCAATGC TATTAAGTGG CTTGATGAGG GTCGCAAGGG TGTTGTAGGG 
ATCGTACCCA ACCTCACTGC AGCCCATACA GTACTGAGCA AATCTGGTTT AGACTGTCAA 
TTGACCGGTT TCAGAGAAGA TCTAAGCGTC TACGTCTGCA CTTCCTACAG TGATGCACAG 
TGTGCAGAGA TTCAGGAATT TGTTGCAGAA GGTGGAGGTC TTCTTATTGG GGGTCATGCC 
TGGTACTGGG CTCAGACCCA CTGGGGCTCC AATGTGATGA CTGAATACCC CGGAAATCAC 
ATTCTTAACA AGATGGGATT GTGCCTCTTG GGAAACACCT TGGGTGGAGG GTTATATAAA 
GCTCCAGAAA TAGAGCATAG TTGTAAACAT GGGTACCACT TTCGAAGCAT GCTGCAGAGA 
TTTGCTCAGC ATGTAAGCCA AGGGCAAGAG CTGACTGATC GAGAACAGGG CTGTTTGAAG 
CAGCTGGGCA CTGACTGTGC CAGTTACCTT CAAATGCGTT GTCATGACAG TGCTGCTTAC 
ACCTCAGTGG TAGCTCTTCT TAGTGACATA GTAAAGGAAG TAGGTGTTCC ACAGGTGTGT 
AGTAACTGCC CTGTTGAAAG TCCCAAAGAC CACCTGATGC TCCACATTGG TACTGAGGTC 
TACAAAGTGA CGCCTGATCC CGACGCCCTT CTTCCCTACA TCATCAAGGA CAGACCCAAC 
TTGCCAACTT TGTCAAATGC AAGAGTTCGG ATTACTGCCA ACACTGGAGG TTGTGAAGAA 
TGGATAAGCA CAGGCTTATA TCTTTCTCCT GGTATGAAGA CATACATAGC AGTACCTCCA 
GAGATTGTTG GAAAAAATTG GCAGGTGCAG CTTGGTTGCC AGACAGACAA CATTGGTGGA 
TCAAACACCC TGAAGCGGGC ACCTGTAGTC CATGCACGAT TCCCCTTGGA TTCAGAAATG 
GTGCAGGTCT GGAATTTGTG GGGAGGGCTA ATCTACCTAA TAGCACCTTC CCAAACCAAG 
GTGGATGGGG TGGAAATTGT TGTGCAGACT GCAGTACAGG CTCCATACTT CAAGTCTGGA 
GAGACAAGCG TAGCAGACTG GGTGAGCCAT ATTCGTCAGG CACCTGCCCC CTGGGCGGAG 
CTTGAGTTTG AAAACCTCAT CATGACATTT GATTCAGCTT TCATAAGGAA TCTGGACCGC 
CCTGATGAAG TGGCTAAACT TTGGGATACC ATAATGAGAA CTATAACTGA CTTGGCAGCA 
AGGCCACCCA AATTGCCTCG CAAGGAACGT TTTGTTGCTG ATGTTCAGAT CTCTTATGGT 
TTCATGCATG CTGGATATCC CATAATGATG CACTCCGGCT CTGCTCCAGG ACTAGTAAAT 
GTTGAGGAAG CCTATAAATG TGGCCTCTGG GGAGCCATTC ATGAGCTGGG CCATAACCAG 
CAGCGTGGAG TTTGGGAGTT CCCTCCCCAC ACCACTGAGT GCACATGCAA CCTGTGGTCA 
TTGTATGTAC ATGAGCAAGT GTTCGGCATA AAAAGCGCTA ATGCTCATCC AGCCATCACT 
CCAGCAGACC GCCAGGCTCG TACCAAGATG TATTTTGATG GTGGTAAAGA TTTAAACAGC 
TGGTGCATGT GGATGGCACT TGAGACTTAC ATGCAGCTTC AAGAAAAATT TGGCTGGGAT 
GCTTTCAAGA AAGTCTTTTC TCTCTACCAT GACATGACCG GTGTGCCAAA TGACAACGCA 
GGGAAGATGA ATCTATACGC CCAGACCTTC TCCAAAGTGG TCAACCTGAA TTTATCCCCA 
TTCTTCAAAG CATGGGGTTG GCCCATCCAA CCTAACACAG AACAGAATCT CTCCCATCTT 
CCTGCATGGA GTGACCATCC TGTTAGCCAG TATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.
The Journal of cell biology208(1)89-107(2015 Jan)
Gkika D,Lemonnier L,Shapovalov G,Gordienko D,Poux C,Bernardini M,Bokhobza A,Bidaux G,Degerny C,Verreman K,Guarmit B,Benahmed M,de Launoit Y,Bindels RJ,Fiorio Pla A,Prevarskaya N

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ZGC:162193 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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