LOC100489234 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following LOC100489234 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100489234 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa53016 XM_031897382.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53017 XM_031897383.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53018 XM_031897384.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53019 XM_031897385.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53020 XM_031897386.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53020 XM_031897387.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53021 XM_031897388.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53022 XM_031897389.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53013 XM_031897379.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53014 XM_031897380.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53015 XM_031897381.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa41059 XM_018089944.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa53016
    Clone ID Related Accession (Same CDS sequence) XM_031897382.1
    Accession Version XM_031897382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATCCGGTT CCGTGCAAAC TGCTGCTGAG AAAACCAACT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCACTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAG GGTTATCTCA ACACAGAGGA GTTTATGGCT
    TTTCTGAAAA ATGCTGTATC TTGGCTGAGT CCTAATTCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CAATAATCTA TCTGGTGCCG GGTACAAAGT CCGAAATACT
    TCCACCCTGA TTCAGGGCCT GGGCGTGTTC TGCACTAACG GATATGAGGC CCACCAAGCT
    GAGGAGATCA TCTCATTTGT GCGAGGGGGA GGCGGCCTGC TGATTGGGGC GCAGGCTTGG
    CACTGGTCCT ACAGCCATAA AGAGAATGTT CTGCACCATT TCCCAGGGAA CAAAATCATA
    TCTGTTTGTG GGGTGCACTT CACCAGTGAG TACGGAGAGA AAGGAGATTT CTGGGTGACT
    GATGAGATGC CGCAGGCTCC AGTGTGCACA GAGAATATGG CGGCTGATGA AGATTACCAG
    TCTCTGGTCC GTGGCGTTGC GTCTCTGAGT TTCACTGGGG ATGCGGTTCC GTGCAAACTG
    CTGCTGACAG GACCCTGTGC ATTCCCGGTG CTGGTGTCGC CCCGAAAAGA TGTGCTCATC
    GCTGCCTCTC GCTATGGGAA AGGGAAAGTA GTGGTTCTGG CCCATGAGGG TTATCTTAGT
    ACACAGGAGT TTATGGGCTT TTTAAAAAAT GCTGTATCTT GGCTGAGGCC AAACCCAGAT
    GCCGTTATTG GGGTTCATAC TACATTAACA TTGTTAGCCC ATAATCTATT TGGGTACAAA
    ATCCGAAATA CTTCCACCCT GATTCAGGGC CTGGGCGTGT TCTGCACTAA CGGATATGAG
    GCCCACCAAG CTGAGGAGAT CATCTCATTT GTGCGAGGGG GAGGCGGCCT GCTGATTGGG
    GCGCAGGCTT GGCACTGGTC CTACAGCCAT AAAGAGAATG TTCTGCACCA TTTCCCAGGG
    AACAAAATCA TATCTGTTTG TGGGGTGCAC TTCACCAGTG AGTACGGAGA GAAAGGAGAT
    TTCTGGGTGA CTGATGAGAT GCCGCAGGCT CCAGTGTGCA CAGAGAATAT GGCGGCTGAT
    GAAGATTACC AGTCTCTGGT CCGTGGTGTT GCGTCTCTGA GTTTCACTGG GGATGCGGTT
    CCGTGCAAAC TGCTGCTGAC AGGACCCTGT GCATTCCCGG TGCTGGTGTC GCCCCGAAAA
    GATGTGCTCA TCGCTGCCTC TCGCTATGGG AAAGGGAAAG TAGTGGTTCT GGCCCATGAG
    GGTTATCTTA GTACACAGGA GTTTATGGGC TTTTTAAAAA ATGCTGTATC TTGGCTGAGG
    CCAAACCCAG ATGTCATTAT TGGGGTTCAT ACTACATTAC CAGTGTTAGC CCATAATCTA
    TCTGGGTACA AAGTCCGAAA TACTTCCACC CTGATTCAGG GCCTGGGCGT GTTCTGCACT
    AACGGATATG AGGCCCACCA AGCTGAGGAG ATCATCTCAT TTGTGCGAGG GGGAGGCGGC
    CTGCTGATTG GGGCGCAGGC TTGGCACTGG TCCTACAGCC ATAAAGAGAA TGTTCTGCAC
    CATTTCCCAG GGAACAAAAT CATATCTGTT TGTGGGGTGC ACTTCACCAG TGAGTACGGA
    GAGAAAGGAG ATTTCTTGGT GACTGAAGAG ATGCCGCAGG CTCCAGTGTG CACAGATTAT
    GATTTCTCAG TGGGTCAGAA GTTCCTCCTG AATGGCGTCT CTCAGCTGGA CATCAGTGGG
    CCGAGTGTCC CCTCAGACCC CCTCTTGCAC GGCGCTTCGT CCTTCCCAGT TGGGTCGAGT
    GAAGACCAAC AGTGCTTTGT TGGAGCTGCA TATTATGGCC AAGGGCGAGT GGTTGTAGCC
    ACACATGAAG ATTTCCTCTC CAAACCAGAA CTGAAGACTT TAATGCTTAA TGCCATATCT
    TGGCTGGACG TAGGGCAGAG AAGAATCGGA GTCCATGCCG ACCTGAGGGG CTTTGCTGAA
    GTTCTGCAGA AGGAAAACAT CCCCTGTCGG GTATCAAGTT TAACCCCCAA GCTGAGTGTC
    TACTGCTGCC CGTCCTACAG CGACGCTGAA GCAAAGGCCG TCCATCAGTT TGTTGCAGAG
    GGGGGAGGGC TTCTTATCGC TGGCCATGCC TGGTGGTGTT CCTATCAGAA TTCAGCCTGC
    AATGTCCTCA ACTACCCTGG AAACAAAATA CTGAACAGGT TTGGGATTAG CATTCTGTGT
    AGAGCCGTCC CTCAGGGCAT CTATTGGGCA GTCAGTCCCA ATGAAGCCAG TCACCAAAAT
    AACTGCCACA GAGCACTTCA TCATTTGCAG GTTGCACTAA AGAGCGGTAC CAAACTGCTA
    CAGCCCATCA GCACCAGGAT GCGCAAACTC AGGCAGAATG TCAGCGCTTT TATGAGGCAC
    TCGGCCTGTT GTGTTCCCAA TGTCAGCAAG ACTTGTCCTG TGAGCGGCTG CTCCGAAGAA
    GCTTTACTGC TGAGTTTGGC CCACAAATCC CATAATATGA GCCACAAGTG CGATGAATTG
    AGTATTCTGG AGAAGGAAAC AGTCACTGTT AATATTGATG CCACCAACCC AGGTGCCGAC
    GCTTGGAGAA GCACCGGCCT TTATTTGCCC CCCAGAAAGG CAGTGGTTAT TGAGTTCCCG
    GCCTCAGCCG TTCAGCAAGG TTTCCAGGTG CAGGTCGGCT GTCACTCAGA TGATCCGAGC
    GCTAAAGAAA CGCTTAATCG AGCGCCGGCC GTTGTTCGCA GGATTCATGT TGATCGCCAG
    AAGGTTCCAG TGTCCAGTTT CTGGGGAGGC CTCCTGTACA TCATTGTGAG GTCTAACAGC
    AACTTTGGTA CCGTCCCAGT CAGGGTGTAT GGAGCAGAAC CAGCCCCAAT CTATATAAAG
    GGCAAAACCA GCCTGGATTC CTGGTTACAT TCAATCCGCC AGTTGCCTGC CCCTTGGGCC
    GAGCTGGTTG CAGAGAACAT TATCCTGACT GTCCCATCAG ATGCCGTTCG CTCACTAAAG
    AATCCAGAAG CCCTGCTGTC TCTTTGGGAC AGGATCATGG CGGCTATTGC TGACCTTGCA
    GCCAAACCCA AGAAATTTCC ACGACCTGAG CGTATTGTTG CAGATGTGCA GATCTCAGCT
    GGTTGGATGC ATGCTGGATA CCCCATCATG TGTGACTTGG AATCGGCTAA AGAGCTGACG
    GATCTACACC ATATGGAGAA AGAGGGGATC TGGGGGCCAA TTCATGAACT TGGCCACAAC
    CAACAGAAAA CACAGTGGGA GTTCCCACCC CATACCACGG AAGCCACGTG TAACCTCTGG
    GCTGTGTATG TGCATGAAAC GGTCCTTGGG ATCCCGCGGG ATAGAGCCCA CGGCAACCTT
    CAGCCCAACG TCCGGACGTC TCGTATCAAA AGTTACTTGC AGAATGGAGC CAACTTAGAG
    CAGTGGGATG GATGGACAGC GTTGGAGACC TACCTACAGC TGCAGGAAGG CTTGGGCTGG
    GACCCATTCA GGCGCCTATA CAGAGACCCG AGCATCTCCG GCATGAGCAA TGAAAACACA
    TTCAAGATGA ATCTGTGGGC GGAGAAGTTC TCTCGGGCCG CGCAGACCAA TCTGGTTCCG
    TTCTTTGAGG CCTGGGGATG GCCCATTGAC CAATTGACTC GCAGTAAACT CTCCGCATTG
    CCCGTATGGG AGAAGGATCC CATGAAGCCC TATGTTACTG CCAAGAAGTC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753242.1
    CDS109..4605
    Translation

    Target ORF information:

    RefSeq Version XM_031897382.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATCCGGTT CCGTGCAAAC TGCTGCTGAG AAAACCAACT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCACTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAG GGTTATCTCA ACACAGAGGA GTTTATGGCT
    TTTCTGAAAA ATGCTGTATC TTGGCTGAGT CCTAATTCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CAATAATCTA TCTGGTGCCG GGTACAAAGT CCGAAATACT
    TCCACCCTGA TTCAGGGCCT GGGCGTGTTC TGCACTAACG GATATGAGGC CCACCAAGCT
    GAGGAGATCA TCTCATTTGT GCGAGGGGGA GGCGGCCTGC TGATTGGGGC GCAGGCTTGG
    CACTGGTCCT ACAGCCATAA AGAGAATGTT CTGCACCATT TCCCAGGGAA CAAAATCATA
    TCTGTTTGTG GGGTGCACTT CACCAGTGAG TACGGAGAGA AAGGAGATTT CTGGGTGACT
    GATGAGATGC CGCAGGCTCC AGTGTGCACA GAGAATATGG CGGCTGATGA AGATTACCAG
    TCTCTGGTCC GTGGCGTTGC GTCTCTGAGT TTCACTGGGG ATGCGGTTCC GTGCAAACTG
    CTGCTGACAG GACCCTGTGC ATTCCCGGTG CTGGTGTCGC CCCGAAAAGA TGTGCTCATC
    GCTGCCTCTC GCTATGGGAA AGGGAAAGTA GTGGTTCTGG CCCATGAGGG TTATCTTAGT
    ACACAGGAGT TTATGGGCTT TTTAAAAAAT GCTGTATCTT GGCTGAGGCC AAACCCAGAT
    GCCGTTATTG GGGTTCATAC TACATTAACA TTGTTAGCCC ATAATCTATT TGGGTACAAA
    ATCCGAAATA CTTCCACCCT GATTCAGGGC CTGGGCGTGT TCTGCACTAA CGGATATGAG
    GCCCACCAAG CTGAGGAGAT CATCTCATTT GTGCGAGGGG GAGGCGGCCT GCTGATTGGG
    GCGCAGGCTT GGCACTGGTC CTACAGCCAT AAAGAGAATG TTCTGCACCA TTTCCCAGGG
    AACAAAATCA TATCTGTTTG TGGGGTGCAC TTCACCAGTG AGTACGGAGA GAAAGGAGAT
    TTCTGGGTGA CTGATGAGAT GCCGCAGGCT CCAGTGTGCA CAGAGAATAT GGCGGCTGAT
    GAAGATTACC AGTCTCTGGT CCGTGGTGTT GCGTCTCTGA GTTTCACTGG GGATGCGGTT
    CCGTGCAAAC TGCTGCTGAC AGGACCCTGT GCATTCCCGG TGCTGGTGTC GCCCCGAAAA
    GATGTGCTCA TCGCTGCCTC TCGCTATGGG AAAGGGAAAG TAGTGGTTCT GGCCCATGAG
    GGTTATCTTA GTACACAGGA GTTTATGGGC TTTTTAAAAA ATGCTGTATC TTGGCTGAGG
    CCAAACCCAG ATGTCATTAT TGGGGTTCAT ACTACATTAC CAGTGTTAGC CCATAATCTA
    TCTGGGTACA AAGTCCGAAA TACTTCCACC CTGATTCAGG GCCTGGGCGT GTTCTGCACT
    AACGGATATG AGGCCCACCA AGCTGAGGAG ATCATCTCAT TTGTGCGAGG GGGAGGCGGC
    CTGCTGATTG GGGCGCAGGC TTGGCACTGG TCCTACAGCC ATAAAGAGAA TGTTCTGCAC
    CATTTCCCAG GGAACAAAAT CATATCTGTT TGTGGGGTGC ACTTCACCAG TGAGTACGGA
    GAGAAAGGAG ATTTCTTGGT GACTGAAGAG ATGCCGCAGG CTCCAGTGTG CACAGATTAT
    GATTTCTCAG TGGGTCAGAA GTTCCTCCTG AATGGCGTCT CTCAGCTGGA CATCAGTGGG
    CCGAGTGTCC CCTCAGACCC CCTCTTGCAC GGCGCTTCGT CCTTCCCAGT TGGGTCGAGT
    GAAGACCAAC AGTGCTTTGT TGGAGCTGCA TATTATGGCC AAGGGCGAGT GGTTGTAGCC
    ACACATGAAG ATTTCCTCTC CAAACCAGAA CTGAAGACTT TAATGCTTAA TGCCATATCT
    TGGCTGGACG TAGGGCAGAG AAGAATCGGA GTCCATGCCG ACCTGAGGGG CTTTGCTGAA
    GTTCTGCAGA AGGAAAACAT CCCCTGTCGG GTATCAAGTT TAACCCCCAA GCTGAGTGTC
    TACTGCTGCC CGTCCTACAG CGACGCTGAA GCAAAGGCCG TCCATCAGTT TGTTGCAGAG
    GGGGGAGGGC TTCTTATCGC TGGCCATGCC TGGTGGTGTT CCTATCAGAA TTCAGCCTGC
    AATGTCCTCA ACTACCCTGG AAACAAAATA CTGAACAGGT TTGGGATTAG CATTCTGTGT
    AGAGCCGTCC CTCAGGGCAT CTATTGGGCA GTCAGTCCCA ATGAAGCCAG TCACCAAAAT
    AACTGCCACA GAGCACTTCA TCATTTGCAG GTTGCACTAA AGAGCGGTAC CAAACTGCTA
    CAGCCCATCA GCACCAGGAT GCGCAAACTC AGGCAGAATG TCAGCGCTTT TATGAGGCAC
    TCGGCCTGTT GTGTTCCCAA TGTCAGCAAG ACTTGTCCTG TGAGCGGCTG CTCCGAAGAA
    GCTTTACTGC TGAGTTTGGC CCACAAATCC CATAATATGA GCCACAAGTG CGATGAATTG
    AGTATTCTGG AGAAGGAAAC AGTCACTGTT AATATTGATG CCACCAACCC AGGTGCCGAC
    GCTTGGAGAA GCACCGGCCT TTATTTGCCC CCCAGAAAGG CAGTGGTTAT TGAGTTCCCG
    GCCTCAGCCG TTCAGCAAGG TTTCCAGGTG CAGGTCGGCT GTCACTCAGA TGATCCGAGC
    GCTAAAGAAA CGCTTAATCG AGCGCCGGCC GTTGTTCGCA GGATTCATGT TGATCGCCAG
    AAGGTTCCAG TGTCCAGTTT CTGGGGAGGC CTCCTGTACA TCATTGTGAG GTCTAACAGC
    AACTTTGGTA CCGTCCCAGT CAGGGTGTAT GGAGCAGAAC CAGCCCCAAT CTATATAAAG
    GGCAAAACCA GCCTGGATTC CTGGTTACAT TCAATCCGCC AGTTGCCTGC CCCTTGGGCC
    GAGCTGGTTG CAGAGAACAT TATCCTGACT GTCCCATCAG ATGCCGTTCG CTCACTAAAG
    AATCCAGAAG CCCTGCTGTC TCTTTGGGAC AGGATCATGG CGGCTATTGC TGACCTTGCA
    GCCAAACCCA AGAAATTTCC ACGACCTGAG CGTATTGTTG CAGATGTGCA GATCTCAGCT
    GGTTGGATGC ATGCTGGATA CCCCATCATG TGTGACTTGG AATCGGCTAA AGAGCTGACG
    GATCTACACC ATATGGAGAA AGAGGGGATC TGGGGGCCAA TTCATGAACT TGGCCACAAC
    CAACAGAAAA CACAGTGGGA GTTCCCACCC CATACCACGG AAGCCACGTG TAACCTCTGG
    GCTGTGTATG TGCATGAAAC GGTCCTTGGG ATCCCGCGGG ATAGAGCCCA CGGCAACCTT
    CAGCCCAACG TCCGGACGTC TCGTATCAAA AGTTACTTGC AGAATGGAGC CAACTTAGAG
    CAGTGGGATG GATGGACAGC GTTGGAGACC TACCTACAGC TGCAGGAAGG CTTGGGCTGG
    GACCCATTCA GGCGCCTATA CAGAGACCCG AGCATCTCCG GCATGAGCAA TGAAAACACA
    TTCAAGATGA ATCTGTGGGC GGAGAAGTTC TCTCGGGCCG CGCAGACCAA TCTGGTTCCG
    TTCTTTGAGG CCTGGGGATG GCCCATTGAC CAATTGACTC GCAGTAAACT CTCCGCATTG
    CCCGTATGGG AGAAGGATCC CATGAAGCCC TATGTTACTG CCAAGAAGTC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53017
    Clone ID Related Accession (Same CDS sequence) XM_031897383.1
    Accession Version XM_031897383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGAGAAT ATGGCGGCTG ATGAAGATTA CCAGTCTCTG
    GTCCGTGGCG TTGCGTCTCT GAGTTTCACT GGGGATGCGG TTCCGTGCAA ACTGCTGCTG
    ACAGGACCCT GTGCATTCCC GGTGCTGGTG TCGCCCCGAA AAGATGTGCT CATCGCTGCC
    TCTCGCTATG GGAAAGGGAA AGTAGTGGTT CTGGCCCATG AGGGTTATCT TAGTACACAG
    GAGTTTATGG GCTTTTTAAA AAATGCTGTA TCTTGGCTGA GGCCAAACCC AGATGCCGTT
    ATTGGGGTTC ATACTACATT AACATTGTTA GCCCATAATC TATTTGGGTA CAAAATCCGA
    AATACTTCCA CCCTGATTCA GGGCCTGGGC GTGTTCTGCA CTAACGGATA TGAGGCCCAC
    CAAGCTGAGG AGATCATCTC ATTTGTGCGA GGGGGAGGCG GCCTGCTGAT TGGGGCGCAG
    GCTTGGCACT GGTCCTACAG CCATAAAGAG AATGTTCTGC ACCATTTCCC AGGGAACAAA
    ATCATATCTG TTTGTGGGGT GCACTTCACC AGTGAGTACG GAGAGAAAGG AGATTTCTGG
    GTGACTGATG AGATGCCGCA GGCTCCAGTG TGCACAGAGA ATATGGCGGC TGATGAAGAT
    TACCAGTCTC TGGTCCGTGG TGTTGCGTCT CTGAGTTTCA CTGGGGATGC GGTTCCGTGC
    AAACTGCTGC TGACAGGACC CTGTGCATTC CCGGTGCTGG TGTCGCCCCG AAAAGATGTG
    CTCATCGCTG CCTCTCGCTA TGGGAAAGGG AAAGTAGTGG TTCTGGCCCA TGAGGGTTAT
    CTTAGTACAC AGGAGTTTAT GGGCTTTTTA AAAAATGCTG TATCTTGGCT GAGGCCAAAC
    CCAGATGTCA TTATTGGGGT TCATACTACA TTACCAGTGT TAGCCCATAA TCTATCTGGG
    TACAAAGTCC GAAATACTTC CACCCTGATT CAGGGCCTGG GCGTGTTCTG CACTAACGGA
    TATGAGGCCC ACCAAGCTGA GGAGATCATC TCATTTGTGC GAGGGGGAGG CGGCCTGCTG
    ATTGGGGCGC AGGCTTGGCA CTGGTCCTAC AGCCATAAAG AGAATGTTCT GCACCATTTC
    CCAGGGAACA AAATCATATC TGTTTGTGGG GTGCACTTCA CCAGTGAGTA CGGAGAGAAA
    GGAGATTTCT TGGTGACTGA AGAGATGCCG CAGGCTCCAG TGTGCACAGA TTATGATTTC
    TCAGTGGGTC AGAAGTTCCT CCTGAATGGC GTCTCTCAGC TGGACATCAG TGGGCCGAGT
    GTCCCCTCAG ACCCCCTCTT GCACGGCGCT TCGTCCTTCC CAGTTGGGTC GAGTGAAGAC
    CAACAGTGCT TTGTTGGAGC TGCATATTAT GGCCAAGGGC GAGTGGTTGT AGCCACACAT
    GAAGATTTCC TCTCCAAACC AGAACTGAAG ACTTTAATGC TTAATGCCAT ATCTTGGCTG
    GACGTAGGGC AGAGAAGAAT CGGAGTCCAT GCCGACCTGA GGGGCTTTGC TGAAGTTCTG
    CAGAAGGAAA ACATCCCCTG TCGGGTATCA AGTTTAACCC CCAAGCTGAG TGTCTACTGC
    TGCCCGTCCT ACAGCGACGC TGAAGCAAAG GCCGTCCATC AGTTTGTTGC AGAGGGGGGA
    GGGCTTCTTA TCGCTGGCCA TGCCTGGTGG TGTTCCTATC AGAATTCAGC CTGCAATGTC
    CTCAACTACC CTGGAAACAA AATACTGAAC AGGTTTGGGA TTAGCATTCT GTGTAGAGCC
    GTCCCTCAGG GCATCTATTG GGCAGTCAGT CCCAATGAAG CCAGTCACCA AAATAACTGC
    CACAGAGCAC TTCATCATTT GCAGGTTGCA CTAAAGAGCG GTACCAAACT GCTACAGCCC
    ATCAGCACCA GGATGCGCAA ACTCAGGCAG AATGTCAGCG CTTTTATGAG GCACTCGGCC
    TGTTGTGTTC CCAATGTCAG CAAGACTTGT CCTGTGAGCG GCTGCTCCGA AGAAGCTTTA
    CTGCTGAGTT TGGCCCACAA ATCCCATAAT ATGAGCCACA AGTGCGATGA ATTGAGTATT
    CTGGAGAAGG AAACAGTCAC TGTTAATATT GATGCCACCA ACCCAGGTGC CGACGCTTGG
    AGAAGCACCG GCCTTTATTT GCCCCCCAGA AAGGCAGTGG TTATTGAGTT CCCGGCCTCA
    GCCGTTCAGC AAGGTTTCCA GGTGCAGGTC GGCTGTCACT CAGATGATCC GAGCGCTAAA
    GAAACGCTTA ATCGAGCGCC GGCCGTTGTT CGCAGGATTC ATGTTGATCG CCAGAAGGTT
    CCAGTGTCCA GTTTCTGGGG AGGCCTCCTG TACATCATTG TGAGGTCTAA CAGCAACTTT
    GGTACCGTCC CAGTCAGGGT GTATGGAGCA GAACCAGCCC CAATCTATAT AAAGGGCAAA
    ACCAGCCTGG ATTCCTGGTT ACATTCAATC CGCCAGTTGC CTGCCCCTTG GGCCGAGCTG
    GTTGCAGAGA ACATTATCCT GACTGTCCCA TCAGATGCCG TTCGCTCACT AAAGAATCCA
    GAAGCCCTGC TGTCTCTTTG GGACAGGATC ATGGCGGCTA TTGCTGACCT TGCAGCCAAA
    CCCAAGAAAT TTCCACGACC TGAGCGTATT GTTGCAGATG TGCAGATCTC AGCTGGTTGG
    ATGCATGCTG GATACCCCAT CATGTGTGAC TTGGAATCGG CTAAAGAGCT GACGGATCTA
    CACCATATGG AGAAAGAGGG GATCTGGGGG CCAATTCATG AACTTGGCCA CAACCAACAG
    AAAACACAGT GGGAGTTCCC ACCCCATACC ACGGAAGCCA CGTGTAACCT CTGGGCTGTG
    TATGTGCATG AAACGGTCCT TGGGATCCCG CGGGATAGAG CCCACGGCAA CCTTCAGCCC
    AACGTCCGGA CGTCTCGTAT CAAAAGTTAC TTGCAGAATG GAGCCAACTT AGAGCAGTGG
    GATGGATGGA CAGCGTTGGA GACCTACCTA CAGCTGCAGG AAGGCTTGGG CTGGGACCCA
    TTCAGGCGCC TATACAGAGA CCCGAGCATC TCCGGCATGA GCAATGAAAA CACATTCAAG
    ATGAATCTGT GGGCGGAGAA GTTCTCTCGG GCCGCGCAGA CCAATCTGGT TCCGTTCTTT
    GAGGCCTGGG GATGGCCCAT TGACCAATTG ACTCGCAGTA AACTCTCCGC ATTGCCCGTA
    TGGGAGAAGG ATCCCATGAA GCCCTATGTT ACTGCCAAGA AGTCTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753243.1
    CDS110..4600
    Translation

    Target ORF information:

    RefSeq Version XM_031897383.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGAGAAT ATGGCGGCTG ATGAAGATTA CCAGTCTCTG
    GTCCGTGGCG TTGCGTCTCT GAGTTTCACT GGGGATGCGG TTCCGTGCAA ACTGCTGCTG
    ACAGGACCCT GTGCATTCCC GGTGCTGGTG TCGCCCCGAA AAGATGTGCT CATCGCTGCC
    TCTCGCTATG GGAAAGGGAA AGTAGTGGTT CTGGCCCATG AGGGTTATCT TAGTACACAG
    GAGTTTATGG GCTTTTTAAA AAATGCTGTA TCTTGGCTGA GGCCAAACCC AGATGCCGTT
    ATTGGGGTTC ATACTACATT AACATTGTTA GCCCATAATC TATTTGGGTA CAAAATCCGA
    AATACTTCCA CCCTGATTCA GGGCCTGGGC GTGTTCTGCA CTAACGGATA TGAGGCCCAC
    CAAGCTGAGG AGATCATCTC ATTTGTGCGA GGGGGAGGCG GCCTGCTGAT TGGGGCGCAG
    GCTTGGCACT GGTCCTACAG CCATAAAGAG AATGTTCTGC ACCATTTCCC AGGGAACAAA
    ATCATATCTG TTTGTGGGGT GCACTTCACC AGTGAGTACG GAGAGAAAGG AGATTTCTGG
    GTGACTGATG AGATGCCGCA GGCTCCAGTG TGCACAGAGA ATATGGCGGC TGATGAAGAT
    TACCAGTCTC TGGTCCGTGG TGTTGCGTCT CTGAGTTTCA CTGGGGATGC GGTTCCGTGC
    AAACTGCTGC TGACAGGACC CTGTGCATTC CCGGTGCTGG TGTCGCCCCG AAAAGATGTG
    CTCATCGCTG CCTCTCGCTA TGGGAAAGGG AAAGTAGTGG TTCTGGCCCA TGAGGGTTAT
    CTTAGTACAC AGGAGTTTAT GGGCTTTTTA AAAAATGCTG TATCTTGGCT GAGGCCAAAC
    CCAGATGTCA TTATTGGGGT TCATACTACA TTACCAGTGT TAGCCCATAA TCTATCTGGG
    TACAAAGTCC GAAATACTTC CACCCTGATT CAGGGCCTGG GCGTGTTCTG CACTAACGGA
    TATGAGGCCC ACCAAGCTGA GGAGATCATC TCATTTGTGC GAGGGGGAGG CGGCCTGCTG
    ATTGGGGCGC AGGCTTGGCA CTGGTCCTAC AGCCATAAAG AGAATGTTCT GCACCATTTC
    CCAGGGAACA AAATCATATC TGTTTGTGGG GTGCACTTCA CCAGTGAGTA CGGAGAGAAA
    GGAGATTTCT TGGTGACTGA AGAGATGCCG CAGGCTCCAG TGTGCACAGA TTATGATTTC
    TCAGTGGGTC AGAAGTTCCT CCTGAATGGC GTCTCTCAGC TGGACATCAG TGGGCCGAGT
    GTCCCCTCAG ACCCCCTCTT GCACGGCGCT TCGTCCTTCC CAGTTGGGTC GAGTGAAGAC
    CAACAGTGCT TTGTTGGAGC TGCATATTAT GGCCAAGGGC GAGTGGTTGT AGCCACACAT
    GAAGATTTCC TCTCCAAACC AGAACTGAAG ACTTTAATGC TTAATGCCAT ATCTTGGCTG
    GACGTAGGGC AGAGAAGAAT CGGAGTCCAT GCCGACCTGA GGGGCTTTGC TGAAGTTCTG
    CAGAAGGAAA ACATCCCCTG TCGGGTATCA AGTTTAACCC CCAAGCTGAG TGTCTACTGC
    TGCCCGTCCT ACAGCGACGC TGAAGCAAAG GCCGTCCATC AGTTTGTTGC AGAGGGGGGA
    GGGCTTCTTA TCGCTGGCCA TGCCTGGTGG TGTTCCTATC AGAATTCAGC CTGCAATGTC
    CTCAACTACC CTGGAAACAA AATACTGAAC AGGTTTGGGA TTAGCATTCT GTGTAGAGCC
    GTCCCTCAGG GCATCTATTG GGCAGTCAGT CCCAATGAAG CCAGTCACCA AAATAACTGC
    CACAGAGCAC TTCATCATTT GCAGGTTGCA CTAAAGAGCG GTACCAAACT GCTACAGCCC
    ATCAGCACCA GGATGCGCAA ACTCAGGCAG AATGTCAGCG CTTTTATGAG GCACTCGGCC
    TGTTGTGTTC CCAATGTCAG CAAGACTTGT CCTGTGAGCG GCTGCTCCGA AGAAGCTTTA
    CTGCTGAGTT TGGCCCACAA ATCCCATAAT ATGAGCCACA AGTGCGATGA ATTGAGTATT
    CTGGAGAAGG AAACAGTCAC TGTTAATATT GATGCCACCA ACCCAGGTGC CGACGCTTGG
    AGAAGCACCG GCCTTTATTT GCCCCCCAGA AAGGCAGTGG TTATTGAGTT CCCGGCCTCA
    GCCGTTCAGC AAGGTTTCCA GGTGCAGGTC GGCTGTCACT CAGATGATCC GAGCGCTAAA
    GAAACGCTTA ATCGAGCGCC GGCCGTTGTT CGCAGGATTC ATGTTGATCG CCAGAAGGTT
    CCAGTGTCCA GTTTCTGGGG AGGCCTCCTG TACATCATTG TGAGGTCTAA CAGCAACTTT
    GGTACCGTCC CAGTCAGGGT GTATGGAGCA GAACCAGCCC CAATCTATAT AAAGGGCAAA
    ACCAGCCTGG ATTCCTGGTT ACATTCAATC CGCCAGTTGC CTGCCCCTTG GGCCGAGCTG
    GTTGCAGAGA ACATTATCCT GACTGTCCCA TCAGATGCCG TTCGCTCACT AAAGAATCCA
    GAAGCCCTGC TGTCTCTTTG GGACAGGATC ATGGCGGCTA TTGCTGACCT TGCAGCCAAA
    CCCAAGAAAT TTCCACGACC TGAGCGTATT GTTGCAGATG TGCAGATCTC AGCTGGTTGG
    ATGCATGCTG GATACCCCAT CATGTGTGAC TTGGAATCGG CTAAAGAGCT GACGGATCTA
    CACCATATGG AGAAAGAGGG GATCTGGGGG CCAATTCATG AACTTGGCCA CAACCAACAG
    AAAACACAGT GGGAGTTCCC ACCCCATACC ACGGAAGCCA CGTGTAACCT CTGGGCTGTG
    TATGTGCATG AAACGGTCCT TGGGATCCCG CGGGATAGAG CCCACGGCAA CCTTCAGCCC
    AACGTCCGGA CGTCTCGTAT CAAAAGTTAC TTGCAGAATG GAGCCAACTT AGAGCAGTGG
    GATGGATGGA CAGCGTTGGA GACCTACCTA CAGCTGCAGG AAGGCTTGGG CTGGGACCCA
    TTCAGGCGCC TATACAGAGA CCCGAGCATC TCCGGCATGA GCAATGAAAA CACATTCAAG
    ATGAATCTGT GGGCGGAGAA GTTCTCTCGG GCCGCGCAGA CCAATCTGGT TCCGTTCTTT
    GAGGCCTGGG GATGGCCCAT TGACCAATTG ACTCGCAGTA AACTCTCCGC ATTGCCCGTA
    TGGGAGAAGG ATCCCATGAA GCCCTATGTT ACTGCCAAGA AGTCTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53018
    Clone ID Related Accession (Same CDS sequence) XM_031897384.1
    Accession Version XM_031897384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATCCGGT TCCGTGCAAA CTGCTGCTGA GAAAACCAAC TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCACTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTC AACACAGAGG AGTTTATGGC TTTTCTGAAA
    AATGCTGTAT CTTGGCTGAG TCCTAATTCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    CCAGTGTTAG CCAATAATCT ATCTGGTGCC GGGTACAAAG TCCGAAATAC TTCCACCCTG
    ATTCAGGGCC TGGGCGTGTT CTGCACTAAC GGATATGAGG CCCACCAAGC TGAGGAGATC
    ATCTCATTTG TGCGAGGGGG AGGCGGCCTG CTGATTGGGG CGCAGGCTTG GCACTGGTCC
    TACAGCCATA AAGAGAATGT TCTGCACCAT TTCCCAGGGA ACAAAATCAT ATCTGTTTGT
    GGGGTGCACT TCACCAGTGA GTACGGAGAG AAAGGAGATT TCTGGGTGAC TGATGAGATG
    CCGCAGGCTC CAGTGTGCAC AGAGAATATG GCGGCTGATG AAGATTACCA GTCTCTGGTC
    CGTGGCGTTG CGTCTCTGAG TTTCACTGGG GATGCGGTTC CGTGCAAACT GCTGCTGACA
    GGACCCTGTG CATTCCCGGT GCTGGTGTCG CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT
    CGCTATGGGA AAGGGAAAGT AGTGGTTCTG GCCCATGAGG GTTATCTTAG TACACAGGAG
    TTTATGGGCT TTTTAAAAAA TGCTGTATCT TGGCTGAGGC CAAACCCAGA TGCCGTTATT
    GGGGTTCATA CTACATTAAC ATTGTTAGCC CATAATCTAT TTGGGTACAA AATCCGAAAT
    ACTTCCACCC TGATTCAGGG CCTGGGCGTG TTCTGCACTA ACGGATATGA GGCCCACCAA
    GCTGAGGAGA TCATCTCATT TGTGCGAGGG GGAGGCGGCC TGCTGATTGG GGCGCAGGCT
    TGGCACTGGT CCTACAGCCA TAAAGAGAAT GTTCTGCACC ATTTCCCAGG GAACAAAATC
    ATATCTGTTT GTGGGGTGCA CTTCACCAGT GAGTACGGAG AGAAAGGAGA TTTCTGGGTG
    ACTGATGAGA TGCCGCAGGC TCCAGTGTGC ACAGATTATG ATTTCTCAGT GGGTCAGAAG
    TTCCTCCTGA ATGGCGTCTC TCAGCTGGAC ATCAGTGGGC CGAGTGTCCC CTCAGACCCC
    CTCTTGCACG GCGCTTCGTC CTTCCCAGTT GGGTCGAGTG AAGACCAACA GTGCTTTGTT
    GGAGCTGCAT ATTATGGCCA AGGGCGAGTG GTTGTAGCCA CACATGAAGA TTTCCTCTCC
    AAACCAGAAC TGAAGACTTT AATGCTTAAT GCCATATCTT GGCTGGACGT AGGGCAGAGA
    AGAATCGGAG TCCATGCCGA CCTGAGGGGC TTTGCTGAAG TTCTGCAGAA GGAAAACATC
    CCCTGTCGGG TATCAAGTTT AACCCCCAAG CTGAGTGTCT ACTGCTGCCC GTCCTACAGC
    GACGCTGAAG CAAAGGCCGT CCATCAGTTT GTTGCAGAGG GGGGAGGGCT TCTTATCGCT
    GGCCATGCCT GGTGGTGTTC CTATCAGAAT TCAGCCTGCA ATGTCCTCAA CTACCCTGGA
    AACAAAATAC TGAACAGGTT TGGGATTAGC ATTCTGTGTA GAGCCGTCCC TCAGGGCATC
    TATTGGGCAG TCAGTCCCAA TGAAGCCAGT CACCAAAATA ACTGCCACAG AGCACTTCAT
    CATTTGCAGG TTGCACTAAA GAGCGGTACC AAACTGCTAC AGCCCATCAG CACCAGGATG
    CGCAAACTCA GGCAGAATGT CAGCGCTTTT ATGAGGCACT CGGCCTGTTG TGTTCCCAAT
    GTCAGCAAGA CTTGTCCTGT GAGCGGCTGC TCCGAAGAAG CTTTACTGCT GAGTTTGGCC
    CACAAATCCC ATAATATGAG CCACAAGTGC GATGAATTGA GTATTCTGGA GAAGGAAACA
    GTCACTGTTA ATATTGATGC CACCAACCCA GGTGCCGACG CTTGGAGAAG CACCGGCCTT
    TATTTGCCCC CCAGAAAGGC AGTGGTTATT GAGTTCCCGG CCTCAGCCGT TCAGCAAGGT
    TTCCAGGTGC AGGTCGGCTG TCACTCAGAT GATCCGAGCG CTAAAGAAAC GCTTAATCGA
    GCGCCGGCCG TTGTTCGCAG GATTCATGTT GATCGCCAGA AGGTTCCAGT GTCCAGTTTC
    TGGGGAGGCC TCCTGTACAT CATTGTGAGG TCTAACAGCA ACTTTGGTAC CGTCCCAGTC
    AGGGTGTATG GAGCAGAACC AGCCCCAATC TATATAAAGG GCAAAACCAG CCTGGATTCC
    TGGTTACATT CAATCCGCCA GTTGCCTGCC CCTTGGGCCG AGCTGGTTGC AGAGAACATT
    ATCCTGACTG TCCCATCAGA TGCCGTTCGC TCACTAAAGA ATCCAGAAGC CCTGCTGTCT
    CTTTGGGACA GGATCATGGC GGCTATTGCT GACCTTGCAG CCAAACCCAA GAAATTTCCA
    CGACCTGAGC GTATTGTTGC AGATGTGCAG ATCTCAGCTG GTTGGATGCA TGCTGGATAC
    CCCATCATGT GTGACTTGGA ATCGGCTAAA GAGCTGACGG ATCTACACCA TATGGAGAAA
    GAGGGGATCT GGGGGCCAAT TCATGAACTT GGCCACAACC AACAGAAAAC ACAGTGGGAG
    TTCCCACCCC ATACCACGGA AGCCACGTGT AACCTCTGGG CTGTGTATGT GCATGAAACG
    GTCCTTGGGA TCCCGCGGGA TAGAGCCCAC GGCAACCTTC AGCCCAACGT CCGGACGTCT
    CGTATCAAAA GTTACTTGCA GAATGGAGCC AACTTAGAGC AGTGGGATGG ATGGACAGCG
    TTGGAGACCT ACCTACAGCT GCAGGAAGGC TTGGGCTGGG ACCCATTCAG GCGCCTATAC
    AGAGACCCGA GCATCTCCGG CATGAGCAAT GAAAACACAT TCAAGATGAA TCTGTGGGCG
    GAGAAGTTCT CTCGGGCCGC GCAGACCAAT CTGGTTCCGT TCTTTGAGGC CTGGGGATGG
    CCCATTGACC AATTGACTCG CAGTAAACTC TCCGCATTGC CCGTATGGGA GAAGGATCCC
    ATGAAGCCCT ATGTTACTGC CAAGAAGTCT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753244.1
    CDS110..4585
    Translation

    Target ORF information:

    RefSeq Version XM_031897384.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATCCGGT TCCGTGCAAA CTGCTGCTGA GAAAACCAAC TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCACTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTC AACACAGAGG AGTTTATGGC TTTTCTGAAA
    AATGCTGTAT CTTGGCTGAG TCCTAATTCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    CCAGTGTTAG CCAATAATCT ATCTGGTGCC GGGTACAAAG TCCGAAATAC TTCCACCCTG
    ATTCAGGGCC TGGGCGTGTT CTGCACTAAC GGATATGAGG CCCACCAAGC TGAGGAGATC
    ATCTCATTTG TGCGAGGGGG AGGCGGCCTG CTGATTGGGG CGCAGGCTTG GCACTGGTCC
    TACAGCCATA AAGAGAATGT TCTGCACCAT TTCCCAGGGA ACAAAATCAT ATCTGTTTGT
    GGGGTGCACT TCACCAGTGA GTACGGAGAG AAAGGAGATT TCTGGGTGAC TGATGAGATG
    CCGCAGGCTC CAGTGTGCAC AGAGAATATG GCGGCTGATG AAGATTACCA GTCTCTGGTC
    CGTGGCGTTG CGTCTCTGAG TTTCACTGGG GATGCGGTTC CGTGCAAACT GCTGCTGACA
    GGACCCTGTG CATTCCCGGT GCTGGTGTCG CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT
    CGCTATGGGA AAGGGAAAGT AGTGGTTCTG GCCCATGAGG GTTATCTTAG TACACAGGAG
    TTTATGGGCT TTTTAAAAAA TGCTGTATCT TGGCTGAGGC CAAACCCAGA TGCCGTTATT
    GGGGTTCATA CTACATTAAC ATTGTTAGCC CATAATCTAT TTGGGTACAA AATCCGAAAT
    ACTTCCACCC TGATTCAGGG CCTGGGCGTG TTCTGCACTA ACGGATATGA GGCCCACCAA
    GCTGAGGAGA TCATCTCATT TGTGCGAGGG GGAGGCGGCC TGCTGATTGG GGCGCAGGCT
    TGGCACTGGT CCTACAGCCA TAAAGAGAAT GTTCTGCACC ATTTCCCAGG GAACAAAATC
    ATATCTGTTT GTGGGGTGCA CTTCACCAGT GAGTACGGAG AGAAAGGAGA TTTCTGGGTG
    ACTGATGAGA TGCCGCAGGC TCCAGTGTGC ACAGATTATG ATTTCTCAGT GGGTCAGAAG
    TTCCTCCTGA ATGGCGTCTC TCAGCTGGAC ATCAGTGGGC CGAGTGTCCC CTCAGACCCC
    CTCTTGCACG GCGCTTCGTC CTTCCCAGTT GGGTCGAGTG AAGACCAACA GTGCTTTGTT
    GGAGCTGCAT ATTATGGCCA AGGGCGAGTG GTTGTAGCCA CACATGAAGA TTTCCTCTCC
    AAACCAGAAC TGAAGACTTT AATGCTTAAT GCCATATCTT GGCTGGACGT AGGGCAGAGA
    AGAATCGGAG TCCATGCCGA CCTGAGGGGC TTTGCTGAAG TTCTGCAGAA GGAAAACATC
    CCCTGTCGGG TATCAAGTTT AACCCCCAAG CTGAGTGTCT ACTGCTGCCC GTCCTACAGC
    GACGCTGAAG CAAAGGCCGT CCATCAGTTT GTTGCAGAGG GGGGAGGGCT TCTTATCGCT
    GGCCATGCCT GGTGGTGTTC CTATCAGAAT TCAGCCTGCA ATGTCCTCAA CTACCCTGGA
    AACAAAATAC TGAACAGGTT TGGGATTAGC ATTCTGTGTA GAGCCGTCCC TCAGGGCATC
    TATTGGGCAG TCAGTCCCAA TGAAGCCAGT CACCAAAATA ACTGCCACAG AGCACTTCAT
    CATTTGCAGG TTGCACTAAA GAGCGGTACC AAACTGCTAC AGCCCATCAG CACCAGGATG
    CGCAAACTCA GGCAGAATGT CAGCGCTTTT ATGAGGCACT CGGCCTGTTG TGTTCCCAAT
    GTCAGCAAGA CTTGTCCTGT GAGCGGCTGC TCCGAAGAAG CTTTACTGCT GAGTTTGGCC
    CACAAATCCC ATAATATGAG CCACAAGTGC GATGAATTGA GTATTCTGGA GAAGGAAACA
    GTCACTGTTA ATATTGATGC CACCAACCCA GGTGCCGACG CTTGGAGAAG CACCGGCCTT
    TATTTGCCCC CCAGAAAGGC AGTGGTTATT GAGTTCCCGG CCTCAGCCGT TCAGCAAGGT
    TTCCAGGTGC AGGTCGGCTG TCACTCAGAT GATCCGAGCG CTAAAGAAAC GCTTAATCGA
    GCGCCGGCCG TTGTTCGCAG GATTCATGTT GATCGCCAGA AGGTTCCAGT GTCCAGTTTC
    TGGGGAGGCC TCCTGTACAT CATTGTGAGG TCTAACAGCA ACTTTGGTAC CGTCCCAGTC
    AGGGTGTATG GAGCAGAACC AGCCCCAATC TATATAAAGG GCAAAACCAG CCTGGATTCC
    TGGTTACATT CAATCCGCCA GTTGCCTGCC CCTTGGGCCG AGCTGGTTGC AGAGAACATT
    ATCCTGACTG TCCCATCAGA TGCCGTTCGC TCACTAAAGA ATCCAGAAGC CCTGCTGTCT
    CTTTGGGACA GGATCATGGC GGCTATTGCT GACCTTGCAG CCAAACCCAA GAAATTTCCA
    CGACCTGAGC GTATTGTTGC AGATGTGCAG ATCTCAGCTG GTTGGATGCA TGCTGGATAC
    CCCATCATGT GTGACTTGGA ATCGGCTAAA GAGCTGACGG ATCTACACCA TATGGAGAAA
    GAGGGGATCT GGGGGCCAAT TCATGAACTT GGCCACAACC AACAGAAAAC ACAGTGGGAG
    TTCCCACCCC ATACCACGGA AGCCACGTGT AACCTCTGGG CTGTGTATGT GCATGAAACG
    GTCCTTGGGA TCCCGCGGGA TAGAGCCCAC GGCAACCTTC AGCCCAACGT CCGGACGTCT
    CGTATCAAAA GTTACTTGCA GAATGGAGCC AACTTAGAGC AGTGGGATGG ATGGACAGCG
    TTGGAGACCT ACCTACAGCT GCAGGAAGGC TTGGGCTGGG ACCCATTCAG GCGCCTATAC
    AGAGACCCGA GCATCTCCGG CATGAGCAAT GAAAACACAT TCAAGATGAA TCTGTGGGCG
    GAGAAGTTCT CTCGGGCCGC GCAGACCAAT CTGGTTCCGT TCTTTGAGGC CTGGGGATGG
    CCCATTGACC AATTGACTCG CAGTAAACTC TCCGCATTGC CCGTATGGGA GAAGGATCCC
    ATGAAGCCCT ATGTTACTGC CAAGAAGTCT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53019
    Clone ID Related Accession (Same CDS sequence) XM_031897385.1
    Accession Version XM_031897385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATCCGGT TCCGTGCAAA CTGCTGCTGA GAAAACCAAC TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCACTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTC AACACAGAGG AGTTTATGGC TTTTCTGAAA
    AATGCTGTAT CTTGGCTGAG TCCTAATTCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    CCAGTGTTAG CCAATAATCT ATCTGGTGCC GGGTACAAAG TCCGAAATAC TTCCACCCTG
    ATTCAGGGCC TGGGCGTGTT CTGCACTAAC GGATATGAGG CCCACCAAGC TGAGGAGATC
    ATCTCATTTG TGCGAGGGGG AGGCGGCCTG CTGATTGGGG CGCAGGCTTG GCACTGGTCC
    TACAGCCATA AAGAGAATGT TCTGCACCAT TTCCCAGGGA ACAAAATCAT ATCTGTTTGT
    GGGGTGCACT TCACCAGTGA GTACGGAGAG AAAGGAGATT TCTGGGTGAC TGATGAGATG
    CCGCAGGCTC CAGTGTGCAC AGAGAATATG GCGGCTGATG AAGATTACCA GTCTCTGGTC
    CGTGGTGTTG CGTCTCTGAG TTTCACTGGG GATGCGGTTC CGTGCAAACT GCTGCTGACA
    GGACCCTGTG CATTCCCGGT GCTGGTGTCG CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT
    CGCTATGGGA AAGGGAAAGT AGTGGTTCTG GCCCATGAGG GTTATCTTAG TACACAGGAG
    TTTATGGGCT TTTTAAAAAA TGCTGTATCT TGGCTGAGGC CAAACCCAGA TGTCATTATT
    GGGGTTCATA CTACATTACC AGTGTTAGCC CATAATCTAT CTGGGTACAA AGTCCGAAAT
    ACTTCCACCC TGATTCAGGG CCTGGGCGTG TTCTGCACTA ACGGATATGA GGCCCACCAA
    GCTGAGGAGA TCATCTCATT TGTGCGAGGG GGAGGCGGCC TGCTGATTGG GGCGCAGGCT
    TGGCACTGGT CCTACAGCCA TAAAGAGAAT GTTCTGCACC ATTTCCCAGG GAACAAAATC
    ATATCTGTTT GTGGGGTGCA CTTCACCAGT GAGTACGGAG AGAAAGGAGA TTTCTTGGTG
    ACTGAAGAGA TGCCGCAGGC TCCAGTGTGC ACAGATTATG ATTTCTCAGT GGGTCAGAAG
    TTCCTCCTGA ATGGCGTCTC TCAGCTGGAC ATCAGTGGGC CGAGTGTCCC CTCAGACCCC
    CTCTTGCACG GCGCTTCGTC CTTCCCAGTT GGGTCGAGTG AAGACCAACA GTGCTTTGTT
    GGAGCTGCAT ATTATGGCCA AGGGCGAGTG GTTGTAGCCA CACATGAAGA TTTCCTCTCC
    AAACCAGAAC TGAAGACTTT AATGCTTAAT GCCATATCTT GGCTGGACGT AGGGCAGAGA
    AGAATCGGAG TCCATGCCGA CCTGAGGGGC TTTGCTGAAG TTCTGCAGAA GGAAAACATC
    CCCTGTCGGG TATCAAGTTT AACCCCCAAG CTGAGTGTCT ACTGCTGCCC GTCCTACAGC
    GACGCTGAAG CAAAGGCCGT CCATCAGTTT GTTGCAGAGG GGGGAGGGCT TCTTATCGCT
    GGCCATGCCT GGTGGTGTTC CTATCAGAAT TCAGCCTGCA ATGTCCTCAA CTACCCTGGA
    AACAAAATAC TGAACAGGTT TGGGATTAGC ATTCTGTGTA GAGCCGTCCC TCAGGGCATC
    TATTGGGCAG TCAGTCCCAA TGAAGCCAGT CACCAAAATA ACTGCCACAG AGCACTTCAT
    CATTTGCAGG TTGCACTAAA GAGCGGTACC AAACTGCTAC AGCCCATCAG CACCAGGATG
    CGCAAACTCA GGCAGAATGT CAGCGCTTTT ATGAGGCACT CGGCCTGTTG TGTTCCCAAT
    GTCAGCAAGA CTTGTCCTGT GAGCGGCTGC TCCGAAGAAG CTTTACTGCT GAGTTTGGCC
    CACAAATCCC ATAATATGAG CCACAAGTGC GATGAATTGA GTATTCTGGA GAAGGAAACA
    GTCACTGTTA ATATTGATGC CACCAACCCA GGTGCCGACG CTTGGAGAAG CACCGGCCTT
    TATTTGCCCC CCAGAAAGGC AGTGGTTATT GAGTTCCCGG CCTCAGCCGT TCAGCAAGGT
    TTCCAGGTGC AGGTCGGCTG TCACTCAGAT GATCCGAGCG CTAAAGAAAC GCTTAATCGA
    GCGCCGGCCG TTGTTCGCAG GATTCATGTT GATCGCCAGA AGGTTCCAGT GTCCAGTTTC
    TGGGGAGGCC TCCTGTACAT CATTGTGAGG TCTAACAGCA ACTTTGGTAC CGTCCCAGTC
    AGGGTGTATG GAGCAGAACC AGCCCCAATC TATATAAAGG GCAAAACCAG CCTGGATTCC
    TGGTTACATT CAATCCGCCA GTTGCCTGCC CCTTGGGCCG AGCTGGTTGC AGAGAACATT
    ATCCTGACTG TCCCATCAGA TGCCGTTCGC TCACTAAAGA ATCCAGAAGC CCTGCTGTCT
    CTTTGGGACA GGATCATGGC GGCTATTGCT GACCTTGCAG CCAAACCCAA GAAATTTCCA
    CGACCTGAGC GTATTGTTGC AGATGTGCAG ATCTCAGCTG GTTGGATGCA TGCTGGATAC
    CCCATCATGT GTGACTTGGA ATCGGCTAAA GAGCTGACGG ATCTACACCA TATGGAGAAA
    GAGGGGATCT GGGGGCCAAT TCATGAACTT GGCCACAACC AACAGAAAAC ACAGTGGGAG
    TTCCCACCCC ATACCACGGA AGCCACGTGT AACCTCTGGG CTGTGTATGT GCATGAAACG
    GTCCTTGGGA TCCCGCGGGA TAGAGCCCAC GGCAACCTTC AGCCCAACGT CCGGACGTCT
    CGTATCAAAA GTTACTTGCA GAATGGAGCC AACTTAGAGC AGTGGGATGG ATGGACAGCG
    TTGGAGACCT ACCTACAGCT GCAGGAAGGC TTGGGCTGGG ACCCATTCAG GCGCCTATAC
    AGAGACCCGA GCATCTCCGG CATGAGCAAT GAAAACACAT TCAAGATGAA TCTGTGGGCG
    GAGAAGTTCT CTCGGGCCGC GCAGACCAAT CTGGTTCCGT TCTTTGAGGC CTGGGGATGG
    CCCATTGACC AATTGACTCG CAGTAAACTC TCCGCATTGC CCGTATGGGA GAAGGATCCC
    ATGAAGCCCT ATGTTACTGC CAAGAAGTCT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753245.1
    CDS111..4586
    Translation

    Target ORF information:

    RefSeq Version XM_031897385.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATCCGGT TCCGTGCAAA CTGCTGCTGA GAAAACCAAC TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCACTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTC AACACAGAGG AGTTTATGGC TTTTCTGAAA
    AATGCTGTAT CTTGGCTGAG TCCTAATTCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    CCAGTGTTAG CCAATAATCT ATCTGGTGCC GGGTACAAAG TCCGAAATAC TTCCACCCTG
    ATTCAGGGCC TGGGCGTGTT CTGCACTAAC GGATATGAGG CCCACCAAGC TGAGGAGATC
    ATCTCATTTG TGCGAGGGGG AGGCGGCCTG CTGATTGGGG CGCAGGCTTG GCACTGGTCC
    TACAGCCATA AAGAGAATGT TCTGCACCAT TTCCCAGGGA ACAAAATCAT ATCTGTTTGT
    GGGGTGCACT TCACCAGTGA GTACGGAGAG AAAGGAGATT TCTGGGTGAC TGATGAGATG
    CCGCAGGCTC CAGTGTGCAC AGAGAATATG GCGGCTGATG AAGATTACCA GTCTCTGGTC
    CGTGGTGTTG CGTCTCTGAG TTTCACTGGG GATGCGGTTC CGTGCAAACT GCTGCTGACA
    GGACCCTGTG CATTCCCGGT GCTGGTGTCG CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT
    CGCTATGGGA AAGGGAAAGT AGTGGTTCTG GCCCATGAGG GTTATCTTAG TACACAGGAG
    TTTATGGGCT TTTTAAAAAA TGCTGTATCT TGGCTGAGGC CAAACCCAGA TGTCATTATT
    GGGGTTCATA CTACATTACC AGTGTTAGCC CATAATCTAT CTGGGTACAA AGTCCGAAAT
    ACTTCCACCC TGATTCAGGG CCTGGGCGTG TTCTGCACTA ACGGATATGA GGCCCACCAA
    GCTGAGGAGA TCATCTCATT TGTGCGAGGG GGAGGCGGCC TGCTGATTGG GGCGCAGGCT
    TGGCACTGGT CCTACAGCCA TAAAGAGAAT GTTCTGCACC ATTTCCCAGG GAACAAAATC
    ATATCTGTTT GTGGGGTGCA CTTCACCAGT GAGTACGGAG AGAAAGGAGA TTTCTTGGTG
    ACTGAAGAGA TGCCGCAGGC TCCAGTGTGC ACAGATTATG ATTTCTCAGT GGGTCAGAAG
    TTCCTCCTGA ATGGCGTCTC TCAGCTGGAC ATCAGTGGGC CGAGTGTCCC CTCAGACCCC
    CTCTTGCACG GCGCTTCGTC CTTCCCAGTT GGGTCGAGTG AAGACCAACA GTGCTTTGTT
    GGAGCTGCAT ATTATGGCCA AGGGCGAGTG GTTGTAGCCA CACATGAAGA TTTCCTCTCC
    AAACCAGAAC TGAAGACTTT AATGCTTAAT GCCATATCTT GGCTGGACGT AGGGCAGAGA
    AGAATCGGAG TCCATGCCGA CCTGAGGGGC TTTGCTGAAG TTCTGCAGAA GGAAAACATC
    CCCTGTCGGG TATCAAGTTT AACCCCCAAG CTGAGTGTCT ACTGCTGCCC GTCCTACAGC
    GACGCTGAAG CAAAGGCCGT CCATCAGTTT GTTGCAGAGG GGGGAGGGCT TCTTATCGCT
    GGCCATGCCT GGTGGTGTTC CTATCAGAAT TCAGCCTGCA ATGTCCTCAA CTACCCTGGA
    AACAAAATAC TGAACAGGTT TGGGATTAGC ATTCTGTGTA GAGCCGTCCC TCAGGGCATC
    TATTGGGCAG TCAGTCCCAA TGAAGCCAGT CACCAAAATA ACTGCCACAG AGCACTTCAT
    CATTTGCAGG TTGCACTAAA GAGCGGTACC AAACTGCTAC AGCCCATCAG CACCAGGATG
    CGCAAACTCA GGCAGAATGT CAGCGCTTTT ATGAGGCACT CGGCCTGTTG TGTTCCCAAT
    GTCAGCAAGA CTTGTCCTGT GAGCGGCTGC TCCGAAGAAG CTTTACTGCT GAGTTTGGCC
    CACAAATCCC ATAATATGAG CCACAAGTGC GATGAATTGA GTATTCTGGA GAAGGAAACA
    GTCACTGTTA ATATTGATGC CACCAACCCA GGTGCCGACG CTTGGAGAAG CACCGGCCTT
    TATTTGCCCC CCAGAAAGGC AGTGGTTATT GAGTTCCCGG CCTCAGCCGT TCAGCAAGGT
    TTCCAGGTGC AGGTCGGCTG TCACTCAGAT GATCCGAGCG CTAAAGAAAC GCTTAATCGA
    GCGCCGGCCG TTGTTCGCAG GATTCATGTT GATCGCCAGA AGGTTCCAGT GTCCAGTTTC
    TGGGGAGGCC TCCTGTACAT CATTGTGAGG TCTAACAGCA ACTTTGGTAC CGTCCCAGTC
    AGGGTGTATG GAGCAGAACC AGCCCCAATC TATATAAAGG GCAAAACCAG CCTGGATTCC
    TGGTTACATT CAATCCGCCA GTTGCCTGCC CCTTGGGCCG AGCTGGTTGC AGAGAACATT
    ATCCTGACTG TCCCATCAGA TGCCGTTCGC TCACTAAAGA ATCCAGAAGC CCTGCTGTCT
    CTTTGGGACA GGATCATGGC GGCTATTGCT GACCTTGCAG CCAAACCCAA GAAATTTCCA
    CGACCTGAGC GTATTGTTGC AGATGTGCAG ATCTCAGCTG GTTGGATGCA TGCTGGATAC
    CCCATCATGT GTGACTTGGA ATCGGCTAAA GAGCTGACGG ATCTACACCA TATGGAGAAA
    GAGGGGATCT GGGGGCCAAT TCATGAACTT GGCCACAACC AACAGAAAAC ACAGTGGGAG
    TTCCCACCCC ATACCACGGA AGCCACGTGT AACCTCTGGG CTGTGTATGT GCATGAAACG
    GTCCTTGGGA TCCCGCGGGA TAGAGCCCAC GGCAACCTTC AGCCCAACGT CCGGACGTCT
    CGTATCAAAA GTTACTTGCA GAATGGAGCC AACTTAGAGC AGTGGGATGG ATGGACAGCG
    TTGGAGACCT ACCTACAGCT GCAGGAAGGC TTGGGCTGGG ACCCATTCAG GCGCCTATAC
    AGAGACCCGA GCATCTCCGG CATGAGCAAT GAAAACACAT TCAAGATGAA TCTGTGGGCG
    GAGAAGTTCT CTCGGGCCGC GCAGACCAAT CTGGTTCCGT TCTTTGAGGC CTGGGGATGG
    CCCATTGACC AATTGACTCG CAGTAAACTC TCCGCATTGC CCGTATGGGA GAAGGATCCC
    ATGAAGCCCT ATGTTACTGC CAAGAAGTCT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53020
    Clone ID Related Accession (Same CDS sequence) XM_031897386.1 , XM_031897387.1
    Accession Version XM_031897386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTA ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCGTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AAGGTTATCT TAGTACACAG GAGTTTATGG GCTTTTTAAA TAATGCTGTA
    TCTTGGCTGA GGCCAAACCC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCCATAATC TATCTGGGTA CAAAGTCCGA AATACTTCCA CCCTGATTCA GGGCCTGGGC
    GTGTTCTGCA CTAACGGATA TGAGGCCCAC CAAGCTGAGG AGATCATCTC ATTTGTGCGA
    GGGGGAGGCG GCCTGCTGAT TGGGGCGCAG GCTTGGCACT GGTCCTACAG CCATAAAGAG
    AATGTTCTGC ACCATTTCCC AGGGAACAAA ATCATATCTG TTTGTGGGGT GCACTTCACC
    AGTGAGTACG GAGAGAAAGG AGATTTCTGG GTGACTGAAG AGATGCCGCA GGTTGCAGTG
    TGCACAGATT ACCAGTCTCT GGTCCGTGGC GTTGCGTCTC TGAGTTTCAC TGGGGATCCG
    GTTCCGTGCA AACTGCTGCT GAGAAAACCA ACTGCATTCC CGGTGCTGGT GTCGCCCCGA
    AAAGATGTGC TCATCGCTGC CTCTCACTAT GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT
    GAGGGTTATC TCAACACAGA GGAGTTTATG GCTTTTCTGA AAAATGCTGT ATCTTGGCTG
    AGTCCTAATT CAGATGCCGT TATTGGGGTT CATACTACAT TACCAGTGTT AGCCAATAAT
    CTATCTGGTG CCGGGTACAA AGTCCGAAAT ACTTCCACCC TGATTCAGGG CCTGGGCGTG
    TTCTGCACTA ACGGATATGA GGCCCACCAA GCTGAGGAGA TCATCTCATT TGTGCGAGGG
    GGAGGCGGCC TGCTGATTGG GGCGCAGGCT TGGCACTGGT CCTACAGCCA TAAAGAGAAT
    GTTCTGCACC ATTTCCCAGG GAACAAAATC ATATCTGTTT GTGGGGTGCA CTTCACCAGT
    GAGTACGGAG AGAAAGGAGA TTTCTGGGTG ACTGATGAGA TGCCGCAGGC TCCAGTGTGC
    ACAGAGAATA TGGCGGCTGA TGAAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATGCGGT TCCGTGCAAA CTGCTGCTGA CAGGACCCTG TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCGCTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTT AGTACACAGG AGTTTATGGG CTTTTTAAAA
    AATGCTGTAT CTTGGCTGAG GCCAAACCCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    ACATTGTTAG CCCATAATCT ATTTGGGTAC AAAATCCGAA ATACTTCCAC CCTGATTCAG
    GGCCTGGGCG TGTTCTGCAC TAACGGATAT GAGGCCCACC AAGCTGAGGA GATCATCTCA
    TTTGTGCGAG GGGGAGGCGG CCTGCTGATT GGGGCGCAGG CTTGGCACTG GTCCTACAGC
    CATAAAGAGA ATGTTCTGCA CCATTTCCCA GGGAACAAAA TCATATCTGT TTGTGGGGTG
    CACTTCACCA GTGAGTACGG AGAGAAAGGA GATTTCTGGG TGACTGATGA GATGCCGCAG
    GCTCCAGTGT GCACAGAGAA TATGGCGGCT GATGAAGATT ACCAGTCTCT GGTCCGTGGT
    GTTGCGTCTC TGAGTTTCAC TGGGGATGCG GTTCCGTGCA AACTGCTGCT GACAGGACCC
    TGTGCATTCC CGGTGCTGGT GTCGCCCCGA AAAGATGTGC TCATCGCTGC CTCTCGCTAT
    GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT GAGGGTTATC TTAGTACACA GGAGTTTATG
    GGCTTTTTAA AAAATGCTGT ATCTTGGCTG AGGCCAAACC CAGATGTCAT TATTGGGGTT
    CATACTACAT TACCAGTGTT AGCCCATAAT CTATCTGGGT ACAAAGTCCG AAATACTTCC
    ACCCTGATTC AGGGCCTGGG CGTGTTCTGC ACTAACGGAT ATGAGGCCCA CCAAGCTGAG
    GAGATCATCT CATTTGTGCG AGGGGGAGGC GGCCTGCTGA TTGGGGCGCA GGCTTGGCAC
    TGGTCCTACA GCCATAAAGA GAATGTTCTG CACCATTTCC CAGGGAACAA AATCATATCT
    GTTTGTGGGG TGCACTTCAC CAGTGAGTAC GGAGAGAAAG GAGATTTCTT GGTGACTGAA
    GAGATGCCGC AGGCTCCAGT GTGCACAGAT TATGATTTCT CAGTGGGTCA GAAGTTCCTC
    CTGAATGGCG TCTCTCAGCT GGACATCAGT GGGCCGAGTG TCCCCTCAGA CCCCCTCTTG
    CACGGCGCTT CGTCCTTCCC AGTTGGGTCG AGTGAAGACC AACAGTGCTT TGTTGGAGCT
    GCATATTATG GCCAAGGGCG AGTGGTTGTA GCCACACATG AAGATTTCCT CTCCAAACCA
    GAACTGAAGA CTTTAATGCT TAATGCCATA TCTTGGCTGG ACGTAGGGCA GAGAAGAATC
    GGAGTCCATG CCGACCTGAG GGGCTTTGCT GAAGTTCTGC AGAAGGAAAA CATCCCCTGT
    CGGGTATCAA GTTTAACCCC CAAGCTGAGT GTCTACTGCT GCCCGTCCTA CAGCGACGCT
    GAAGCAAAGG CCGTCCATCA GTTTGTTGCA GAGGGGGGAG GGCTTCTTAT CGCTGGCCAT
    GCCTGGTGGT GTTCCTATCA GAATTCAGCC TGCAATGTCC TCAACTACCC TGGAAACAAA
    ATACTGAACA GGTTTGGGAT TAGCATTCTG TGTAGAGCCG TCCCTCAGGG CATCTATTGG
    GCAGTCAGTC CCAATGAAGC CAGTCACCAA AATAACTGCC ACAGAGCACT TCATCATTTG
    CAGGTTGCAC TAAAGAGCGG TACCAAACTG CTACAGCCCA TCAGCACCAG GATGCGCAAA
    CTCAGGCAGA ATGTCAGCGC TTTTATGAGG CACTCGGCCT GTTGTGTTCC CAATGTCAGC
    AAGACTTGTC CTGTGAGCGG CTGCTCCGAA GAAGCTTTAC TGCTGAGTTT GGCCCACAAA
    TCCCATAATA TGAGCCACAA GTGCGATGAA TTGAGTATTC TGGAGAAGGA AACAGTCACT
    GTTAATATTG ATGCCACCAA CCCAGGTGCC GACGCTTGGA GAAGCACCGG CCTTTATTTG
    CCCCCCAGAA AGGCAGTGGT TATTGAGTTC CCGGCCTCAG CCGTTCAGCA AGGTTTCCAG
    GTGCAGGTCG GCTGTCACTC AGATGATCCG AGCGCTAAAG AAACGCTTAA TCGAGCGCCG
    GCCGTTGTTC GCAGGATTCA TGTTGATCGC CAGAAGGTTC CAGTGTCCAG TTTCTGGGGA
    GGCCTCCTGT ACATCATTGT GAGGTCTAAC AGCAACTTTG GTACCGTCCC AGTCAGGGTG
    TATGGAGCAG AACCAGCCCC AATCTATATA AAGGGCAAAA CCAGCCTGGA TTCCTGGTTA
    CATTCAATCC GCCAGTTGCC TGCCCCTTGG GCCGAGCTGG TTGCAGAGAA CATTATCCTG
    ACTGTCCCAT CAGATGCCGT TCGCTCACTA AAGAATCCAG AAGCCCTGCT GTCTCTTTGG
    GACAGGATCA TGGCGGCTAT TGCTGACCTT GCAGCCAAAC CCAAGAAATT TCCACGACCT
    GAGCGTATTG TTGCAGATGT GCAGATCTCA GCTGGTTGGA TGCATGCTGG ATACCCCATC
    ATGTGTGACT TGGAATCGGC TAAAGAGCTG ACGGATCTAC ACCATATGGA GAAAGAGGGG
    ATCTGGGGGC CAATTCATGA ACTTGGCCAC AACCAACAGA AAACACAGTG GGAGTTCCCA
    CCCCATACCA CGGAAGCCAC GTGTAACCTC TGGGCTGTGT ATGTGCATGA AACGGTCCTT
    GGGATCCCGC GGGATAGAGC CCACGGCAAC CTTCAGCCCA ACGTCCGGAC GTCTCGTATC
    AAAAGTTACT TGCAGAATGG AGCCAACTTA GAGCAGTGGG ATGGATGGAC AGCGTTGGAG
    ACCTACCTAC AGCTGCAGGA AGGCTTGGGC TGGGACCCAT TCAGGCGCCT ATACAGAGAC
    CCGAGCATCT CCGGCATGAG CAATGAAAAC ACATTCAAGA TGAATCTGTG GGCGGAGAAG
    TTCTCTCGGG CCGCGCAGAC CAATCTGGTT CCGTTCTTTG AGGCCTGGGG ATGGCCCATT
    GACCAATTGA CTCGCAGTAA ACTCTCCGCA TTGCCCGTAT GGGAGAAGGA TCCCATGAAG
    CCCTATGTTA CTGCCAAGAA GTCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753246.1
    CDS89..4558
    Translation

    Target ORF information:

    RefSeq Version XM_031897386.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTA ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCGTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AAGGTTATCT TAGTACACAG GAGTTTATGG GCTTTTTAAA TAATGCTGTA
    TCTTGGCTGA GGCCAAACCC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCCATAATC TATCTGGGTA CAAAGTCCGA AATACTTCCA CCCTGATTCA GGGCCTGGGC
    GTGTTCTGCA CTAACGGATA TGAGGCCCAC CAAGCTGAGG AGATCATCTC ATTTGTGCGA
    GGGGGAGGCG GCCTGCTGAT TGGGGCGCAG GCTTGGCACT GGTCCTACAG CCATAAAGAG
    AATGTTCTGC ACCATTTCCC AGGGAACAAA ATCATATCTG TTTGTGGGGT GCACTTCACC
    AGTGAGTACG GAGAGAAAGG AGATTTCTGG GTGACTGAAG AGATGCCGCA GGTTGCAGTG
    TGCACAGATT ACCAGTCTCT GGTCCGTGGC GTTGCGTCTC TGAGTTTCAC TGGGGATCCG
    GTTCCGTGCA AACTGCTGCT GAGAAAACCA ACTGCATTCC CGGTGCTGGT GTCGCCCCGA
    AAAGATGTGC TCATCGCTGC CTCTCACTAT GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT
    GAGGGTTATC TCAACACAGA GGAGTTTATG GCTTTTCTGA AAAATGCTGT ATCTTGGCTG
    AGTCCTAATT CAGATGCCGT TATTGGGGTT CATACTACAT TACCAGTGTT AGCCAATAAT
    CTATCTGGTG CCGGGTACAA AGTCCGAAAT ACTTCCACCC TGATTCAGGG CCTGGGCGTG
    TTCTGCACTA ACGGATATGA GGCCCACCAA GCTGAGGAGA TCATCTCATT TGTGCGAGGG
    GGAGGCGGCC TGCTGATTGG GGCGCAGGCT TGGCACTGGT CCTACAGCCA TAAAGAGAAT
    GTTCTGCACC ATTTCCCAGG GAACAAAATC ATATCTGTTT GTGGGGTGCA CTTCACCAGT
    GAGTACGGAG AGAAAGGAGA TTTCTGGGTG ACTGATGAGA TGCCGCAGGC TCCAGTGTGC
    ACAGAGAATA TGGCGGCTGA TGAAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATGCGGT TCCGTGCAAA CTGCTGCTGA CAGGACCCTG TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCGCTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTT AGTACACAGG AGTTTATGGG CTTTTTAAAA
    AATGCTGTAT CTTGGCTGAG GCCAAACCCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    ACATTGTTAG CCCATAATCT ATTTGGGTAC AAAATCCGAA ATACTTCCAC CCTGATTCAG
    GGCCTGGGCG TGTTCTGCAC TAACGGATAT GAGGCCCACC AAGCTGAGGA GATCATCTCA
    TTTGTGCGAG GGGGAGGCGG CCTGCTGATT GGGGCGCAGG CTTGGCACTG GTCCTACAGC
    CATAAAGAGA ATGTTCTGCA CCATTTCCCA GGGAACAAAA TCATATCTGT TTGTGGGGTG
    CACTTCACCA GTGAGTACGG AGAGAAAGGA GATTTCTGGG TGACTGATGA GATGCCGCAG
    GCTCCAGTGT GCACAGAGAA TATGGCGGCT GATGAAGATT ACCAGTCTCT GGTCCGTGGT
    GTTGCGTCTC TGAGTTTCAC TGGGGATGCG GTTCCGTGCA AACTGCTGCT GACAGGACCC
    TGTGCATTCC CGGTGCTGGT GTCGCCCCGA AAAGATGTGC TCATCGCTGC CTCTCGCTAT
    GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT GAGGGTTATC TTAGTACACA GGAGTTTATG
    GGCTTTTTAA AAAATGCTGT ATCTTGGCTG AGGCCAAACC CAGATGTCAT TATTGGGGTT
    CATACTACAT TACCAGTGTT AGCCCATAAT CTATCTGGGT ACAAAGTCCG AAATACTTCC
    ACCCTGATTC AGGGCCTGGG CGTGTTCTGC ACTAACGGAT ATGAGGCCCA CCAAGCTGAG
    GAGATCATCT CATTTGTGCG AGGGGGAGGC GGCCTGCTGA TTGGGGCGCA GGCTTGGCAC
    TGGTCCTACA GCCATAAAGA GAATGTTCTG CACCATTTCC CAGGGAACAA AATCATATCT
    GTTTGTGGGG TGCACTTCAC CAGTGAGTAC GGAGAGAAAG GAGATTTCTT GGTGACTGAA
    GAGATGCCGC AGGCTCCAGT GTGCACAGAT TATGATTTCT CAGTGGGTCA GAAGTTCCTC
    CTGAATGGCG TCTCTCAGCT GGACATCAGT GGGCCGAGTG TCCCCTCAGA CCCCCTCTTG
    CACGGCGCTT CGTCCTTCCC AGTTGGGTCG AGTGAAGACC AACAGTGCTT TGTTGGAGCT
    GCATATTATG GCCAAGGGCG AGTGGTTGTA GCCACACATG AAGATTTCCT CTCCAAACCA
    GAACTGAAGA CTTTAATGCT TAATGCCATA TCTTGGCTGG ACGTAGGGCA GAGAAGAATC
    GGAGTCCATG CCGACCTGAG GGGCTTTGCT GAAGTTCTGC AGAAGGAAAA CATCCCCTGT
    CGGGTATCAA GTTTAACCCC CAAGCTGAGT GTCTACTGCT GCCCGTCCTA CAGCGACGCT
    GAAGCAAAGG CCGTCCATCA GTTTGTTGCA GAGGGGGGAG GGCTTCTTAT CGCTGGCCAT
    GCCTGGTGGT GTTCCTATCA GAATTCAGCC TGCAATGTCC TCAACTACCC TGGAAACAAA
    ATACTGAACA GGTTTGGGAT TAGCATTCTG TGTAGAGCCG TCCCTCAGGG CATCTATTGG
    GCAGTCAGTC CCAATGAAGC CAGTCACCAA AATAACTGCC ACAGAGCACT TCATCATTTG
    CAGGTTGCAC TAAAGAGCGG TACCAAACTG CTACAGCCCA TCAGCACCAG GATGCGCAAA
    CTCAGGCAGA ATGTCAGCGC TTTTATGAGG CACTCGGCCT GTTGTGTTCC CAATGTCAGC
    AAGACTTGTC CTGTGAGCGG CTGCTCCGAA GAAGCTTTAC TGCTGAGTTT GGCCCACAAA
    TCCCATAATA TGAGCCACAA GTGCGATGAA TTGAGTATTC TGGAGAAGGA AACAGTCACT
    GTTAATATTG ATGCCACCAA CCCAGGTGCC GACGCTTGGA GAAGCACCGG CCTTTATTTG
    CCCCCCAGAA AGGCAGTGGT TATTGAGTTC CCGGCCTCAG CCGTTCAGCA AGGTTTCCAG
    GTGCAGGTCG GCTGTCACTC AGATGATCCG AGCGCTAAAG AAACGCTTAA TCGAGCGCCG
    GCCGTTGTTC GCAGGATTCA TGTTGATCGC CAGAAGGTTC CAGTGTCCAG TTTCTGGGGA
    GGCCTCCTGT ACATCATTGT GAGGTCTAAC AGCAACTTTG GTACCGTCCC AGTCAGGGTG
    TATGGAGCAG AACCAGCCCC AATCTATATA AAGGGCAAAA CCAGCCTGGA TTCCTGGTTA
    CATTCAATCC GCCAGTTGCC TGCCCCTTGG GCCGAGCTGG TTGCAGAGAA CATTATCCTG
    ACTGTCCCAT CAGATGCCGT TCGCTCACTA AAGAATCCAG AAGCCCTGCT GTCTCTTTGG
    GACAGGATCA TGGCGGCTAT TGCTGACCTT GCAGCCAAAC CCAAGAAATT TCCACGACCT
    GAGCGTATTG TTGCAGATGT GCAGATCTCA GCTGGTTGGA TGCATGCTGG ATACCCCATC
    ATGTGTGACT TGGAATCGGC TAAAGAGCTG ACGGATCTAC ACCATATGGA GAAAGAGGGG
    ATCTGGGGGC CAATTCATGA ACTTGGCCAC AACCAACAGA AAACACAGTG GGAGTTCCCA
    CCCCATACCA CGGAAGCCAC GTGTAACCTC TGGGCTGTGT ATGTGCATGA AACGGTCCTT
    GGGATCCCGC GGGATAGAGC CCACGGCAAC CTTCAGCCCA ACGTCCGGAC GTCTCGTATC
    AAAAGTTACT TGCAGAATGG AGCCAACTTA GAGCAGTGGG ATGGATGGAC AGCGTTGGAG
    ACCTACCTAC AGCTGCAGGA AGGCTTGGGC TGGGACCCAT TCAGGCGCCT ATACAGAGAC
    CCGAGCATCT CCGGCATGAG CAATGAAAAC ACATTCAAGA TGAATCTGTG GGCGGAGAAG
    TTCTCTCGGG CCGCGCAGAC CAATCTGGTT CCGTTCTTTG AGGCCTGGGG ATGGCCCATT
    GACCAATTGA CTCGCAGTAA ACTCTCCGCA TTGCCCGTAT GGGAGAAGGA TCCCATGAAG
    CCCTATGTTA CTGCCAAGAA GTCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53020
    Clone ID Related Accession (Same CDS sequence) XM_031897386.1 , XM_031897387.1
    Accession Version XM_031897387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTA ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCGTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AAGGTTATCT TAGTACACAG GAGTTTATGG GCTTTTTAAA TAATGCTGTA
    TCTTGGCTGA GGCCAAACCC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCCATAATC TATCTGGGTA CAAAGTCCGA AATACTTCCA CCCTGATTCA GGGCCTGGGC
    GTGTTCTGCA CTAACGGATA TGAGGCCCAC CAAGCTGAGG AGATCATCTC ATTTGTGCGA
    GGGGGAGGCG GCCTGCTGAT TGGGGCGCAG GCTTGGCACT GGTCCTACAG CCATAAAGAG
    AATGTTCTGC ACCATTTCCC AGGGAACAAA ATCATATCTG TTTGTGGGGT GCACTTCACC
    AGTGAGTACG GAGAGAAAGG AGATTTCTGG GTGACTGAAG AGATGCCGCA GGTTGCAGTG
    TGCACAGATT ACCAGTCTCT GGTCCGTGGC GTTGCGTCTC TGAGTTTCAC TGGGGATCCG
    GTTCCGTGCA AACTGCTGCT GAGAAAACCA ACTGCATTCC CGGTGCTGGT GTCGCCCCGA
    AAAGATGTGC TCATCGCTGC CTCTCACTAT GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT
    GAGGGTTATC TCAACACAGA GGAGTTTATG GCTTTTCTGA AAAATGCTGT ATCTTGGCTG
    AGTCCTAATT CAGATGCCGT TATTGGGGTT CATACTACAT TACCAGTGTT AGCCAATAAT
    CTATCTGGTG CCGGGTACAA AGTCCGAAAT ACTTCCACCC TGATTCAGGG CCTGGGCGTG
    TTCTGCACTA ACGGATATGA GGCCCACCAA GCTGAGGAGA TCATCTCATT TGTGCGAGGG
    GGAGGCGGCC TGCTGATTGG GGCGCAGGCT TGGCACTGGT CCTACAGCCA TAAAGAGAAT
    GTTCTGCACC ATTTCCCAGG GAACAAAATC ATATCTGTTT GTGGGGTGCA CTTCACCAGT
    GAGTACGGAG AGAAAGGAGA TTTCTGGGTG ACTGATGAGA TGCCGCAGGC TCCAGTGTGC
    ACAGAGAATA TGGCGGCTGA TGAAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATGCGGT TCCGTGCAAA CTGCTGCTGA CAGGACCCTG TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCGCTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTT AGTACACAGG AGTTTATGGG CTTTTTAAAA
    AATGCTGTAT CTTGGCTGAG GCCAAACCCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    ACATTGTTAG CCCATAATCT ATTTGGGTAC AAAATCCGAA ATACTTCCAC CCTGATTCAG
    GGCCTGGGCG TGTTCTGCAC TAACGGATAT GAGGCCCACC AAGCTGAGGA GATCATCTCA
    TTTGTGCGAG GGGGAGGCGG CCTGCTGATT GGGGCGCAGG CTTGGCACTG GTCCTACAGC
    CATAAAGAGA ATGTTCTGCA CCATTTCCCA GGGAACAAAA TCATATCTGT TTGTGGGGTG
    CACTTCACCA GTGAGTACGG AGAGAAAGGA GATTTCTGGG TGACTGATGA GATGCCGCAG
    GCTCCAGTGT GCACAGAGAA TATGGCGGCT GATGAAGATT ACCAGTCTCT GGTCCGTGGT
    GTTGCGTCTC TGAGTTTCAC TGGGGATGCG GTTCCGTGCA AACTGCTGCT GACAGGACCC
    TGTGCATTCC CGGTGCTGGT GTCGCCCCGA AAAGATGTGC TCATCGCTGC CTCTCGCTAT
    GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT GAGGGTTATC TTAGTACACA GGAGTTTATG
    GGCTTTTTAA AAAATGCTGT ATCTTGGCTG AGGCCAAACC CAGATGTCAT TATTGGGGTT
    CATACTACAT TACCAGTGTT AGCCCATAAT CTATCTGGGT ACAAAGTCCG AAATACTTCC
    ACCCTGATTC AGGGCCTGGG CGTGTTCTGC ACTAACGGAT ATGAGGCCCA CCAAGCTGAG
    GAGATCATCT CATTTGTGCG AGGGGGAGGC GGCCTGCTGA TTGGGGCGCA GGCTTGGCAC
    TGGTCCTACA GCCATAAAGA GAATGTTCTG CACCATTTCC CAGGGAACAA AATCATATCT
    GTTTGTGGGG TGCACTTCAC CAGTGAGTAC GGAGAGAAAG GAGATTTCTT GGTGACTGAA
    GAGATGCCGC AGGCTCCAGT GTGCACAGAT TATGATTTCT CAGTGGGTCA GAAGTTCCTC
    CTGAATGGCG TCTCTCAGCT GGACATCAGT GGGCCGAGTG TCCCCTCAGA CCCCCTCTTG
    CACGGCGCTT CGTCCTTCCC AGTTGGGTCG AGTGAAGACC AACAGTGCTT TGTTGGAGCT
    GCATATTATG GCCAAGGGCG AGTGGTTGTA GCCACACATG AAGATTTCCT CTCCAAACCA
    GAACTGAAGA CTTTAATGCT TAATGCCATA TCTTGGCTGG ACGTAGGGCA GAGAAGAATC
    GGAGTCCATG CCGACCTGAG GGGCTTTGCT GAAGTTCTGC AGAAGGAAAA CATCCCCTGT
    CGGGTATCAA GTTTAACCCC CAAGCTGAGT GTCTACTGCT GCCCGTCCTA CAGCGACGCT
    GAAGCAAAGG CCGTCCATCA GTTTGTTGCA GAGGGGGGAG GGCTTCTTAT CGCTGGCCAT
    GCCTGGTGGT GTTCCTATCA GAATTCAGCC TGCAATGTCC TCAACTACCC TGGAAACAAA
    ATACTGAACA GGTTTGGGAT TAGCATTCTG TGTAGAGCCG TCCCTCAGGG CATCTATTGG
    GCAGTCAGTC CCAATGAAGC CAGTCACCAA AATAACTGCC ACAGAGCACT TCATCATTTG
    CAGGTTGCAC TAAAGAGCGG TACCAAACTG CTACAGCCCA TCAGCACCAG GATGCGCAAA
    CTCAGGCAGA ATGTCAGCGC TTTTATGAGG CACTCGGCCT GTTGTGTTCC CAATGTCAGC
    AAGACTTGTC CTGTGAGCGG CTGCTCCGAA GAAGCTTTAC TGCTGAGTTT GGCCCACAAA
    TCCCATAATA TGAGCCACAA GTGCGATGAA TTGAGTATTC TGGAGAAGGA AACAGTCACT
    GTTAATATTG ATGCCACCAA CCCAGGTGCC GACGCTTGGA GAAGCACCGG CCTTTATTTG
    CCCCCCAGAA AGGCAGTGGT TATTGAGTTC CCGGCCTCAG CCGTTCAGCA AGGTTTCCAG
    GTGCAGGTCG GCTGTCACTC AGATGATCCG AGCGCTAAAG AAACGCTTAA TCGAGCGCCG
    GCCGTTGTTC GCAGGATTCA TGTTGATCGC CAGAAGGTTC CAGTGTCCAG TTTCTGGGGA
    GGCCTCCTGT ACATCATTGT GAGGTCTAAC AGCAACTTTG GTACCGTCCC AGTCAGGGTG
    TATGGAGCAG AACCAGCCCC AATCTATATA AAGGGCAAAA CCAGCCTGGA TTCCTGGTTA
    CATTCAATCC GCCAGTTGCC TGCCCCTTGG GCCGAGCTGG TTGCAGAGAA CATTATCCTG
    ACTGTCCCAT CAGATGCCGT TCGCTCACTA AAGAATCCAG AAGCCCTGCT GTCTCTTTGG
    GACAGGATCA TGGCGGCTAT TGCTGACCTT GCAGCCAAAC CCAAGAAATT TCCACGACCT
    GAGCGTATTG TTGCAGATGT GCAGATCTCA GCTGGTTGGA TGCATGCTGG ATACCCCATC
    ATGTGTGACT TGGAATCGGC TAAAGAGCTG ACGGATCTAC ACCATATGGA GAAAGAGGGG
    ATCTGGGGGC CAATTCATGA ACTTGGCCAC AACCAACAGA AAACACAGTG GGAGTTCCCA
    CCCCATACCA CGGAAGCCAC GTGTAACCTC TGGGCTGTGT ATGTGCATGA AACGGTCCTT
    GGGATCCCGC GGGATAGAGC CCACGGCAAC CTTCAGCCCA ACGTCCGGAC GTCTCGTATC
    AAAAGTTACT TGCAGAATGG AGCCAACTTA GAGCAGTGGG ATGGATGGAC AGCGTTGGAG
    ACCTACCTAC AGCTGCAGGA AGGCTTGGGC TGGGACCCAT TCAGGCGCCT ATACAGAGAC
    CCGAGCATCT CCGGCATGAG CAATGAAAAC ACATTCAAGA TGAATCTGTG GGCGGAGAAG
    TTCTCTCGGG CCGCGCAGAC CAATCTGGTT CCGTTCTTTG AGGCCTGGGG ATGGCCCATT
    GACCAATTGA CTCGCAGTAA ACTCTCCGCA TTGCCCGTAT GGGAGAAGGA TCCCATGAAG
    CCCTATGTTA CTGCCAAGAA GTCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753247.1
    CDS42..4511
    Translation

    Target ORF information:

    RefSeq Version XM_031897387.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTA ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCGTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AAGGTTATCT TAGTACACAG GAGTTTATGG GCTTTTTAAA TAATGCTGTA
    TCTTGGCTGA GGCCAAACCC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCCATAATC TATCTGGGTA CAAAGTCCGA AATACTTCCA CCCTGATTCA GGGCCTGGGC
    GTGTTCTGCA CTAACGGATA TGAGGCCCAC CAAGCTGAGG AGATCATCTC ATTTGTGCGA
    GGGGGAGGCG GCCTGCTGAT TGGGGCGCAG GCTTGGCACT GGTCCTACAG CCATAAAGAG
    AATGTTCTGC ACCATTTCCC AGGGAACAAA ATCATATCTG TTTGTGGGGT GCACTTCACC
    AGTGAGTACG GAGAGAAAGG AGATTTCTGG GTGACTGAAG AGATGCCGCA GGTTGCAGTG
    TGCACAGATT ACCAGTCTCT GGTCCGTGGC GTTGCGTCTC TGAGTTTCAC TGGGGATCCG
    GTTCCGTGCA AACTGCTGCT GAGAAAACCA ACTGCATTCC CGGTGCTGGT GTCGCCCCGA
    AAAGATGTGC TCATCGCTGC CTCTCACTAT GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT
    GAGGGTTATC TCAACACAGA GGAGTTTATG GCTTTTCTGA AAAATGCTGT ATCTTGGCTG
    AGTCCTAATT CAGATGCCGT TATTGGGGTT CATACTACAT TACCAGTGTT AGCCAATAAT
    CTATCTGGTG CCGGGTACAA AGTCCGAAAT ACTTCCACCC TGATTCAGGG CCTGGGCGTG
    TTCTGCACTA ACGGATATGA GGCCCACCAA GCTGAGGAGA TCATCTCATT TGTGCGAGGG
    GGAGGCGGCC TGCTGATTGG GGCGCAGGCT TGGCACTGGT CCTACAGCCA TAAAGAGAAT
    GTTCTGCACC ATTTCCCAGG GAACAAAATC ATATCTGTTT GTGGGGTGCA CTTCACCAGT
    GAGTACGGAG AGAAAGGAGA TTTCTGGGTG ACTGATGAGA TGCCGCAGGC TCCAGTGTGC
    ACAGAGAATA TGGCGGCTGA TGAAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATGCGGT TCCGTGCAAA CTGCTGCTGA CAGGACCCTG TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCGCTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTT AGTACACAGG AGTTTATGGG CTTTTTAAAA
    AATGCTGTAT CTTGGCTGAG GCCAAACCCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    ACATTGTTAG CCCATAATCT ATTTGGGTAC AAAATCCGAA ATACTTCCAC CCTGATTCAG
    GGCCTGGGCG TGTTCTGCAC TAACGGATAT GAGGCCCACC AAGCTGAGGA GATCATCTCA
    TTTGTGCGAG GGGGAGGCGG CCTGCTGATT GGGGCGCAGG CTTGGCACTG GTCCTACAGC
    CATAAAGAGA ATGTTCTGCA CCATTTCCCA GGGAACAAAA TCATATCTGT TTGTGGGGTG
    CACTTCACCA GTGAGTACGG AGAGAAAGGA GATTTCTGGG TGACTGATGA GATGCCGCAG
    GCTCCAGTGT GCACAGAGAA TATGGCGGCT GATGAAGATT ACCAGTCTCT GGTCCGTGGT
    GTTGCGTCTC TGAGTTTCAC TGGGGATGCG GTTCCGTGCA AACTGCTGCT GACAGGACCC
    TGTGCATTCC CGGTGCTGGT GTCGCCCCGA AAAGATGTGC TCATCGCTGC CTCTCGCTAT
    GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT GAGGGTTATC TTAGTACACA GGAGTTTATG
    GGCTTTTTAA AAAATGCTGT ATCTTGGCTG AGGCCAAACC CAGATGTCAT TATTGGGGTT
    CATACTACAT TACCAGTGTT AGCCCATAAT CTATCTGGGT ACAAAGTCCG AAATACTTCC
    ACCCTGATTC AGGGCCTGGG CGTGTTCTGC ACTAACGGAT ATGAGGCCCA CCAAGCTGAG
    GAGATCATCT CATTTGTGCG AGGGGGAGGC GGCCTGCTGA TTGGGGCGCA GGCTTGGCAC
    TGGTCCTACA GCCATAAAGA GAATGTTCTG CACCATTTCC CAGGGAACAA AATCATATCT
    GTTTGTGGGG TGCACTTCAC CAGTGAGTAC GGAGAGAAAG GAGATTTCTT GGTGACTGAA
    GAGATGCCGC AGGCTCCAGT GTGCACAGAT TATGATTTCT CAGTGGGTCA GAAGTTCCTC
    CTGAATGGCG TCTCTCAGCT GGACATCAGT GGGCCGAGTG TCCCCTCAGA CCCCCTCTTG
    CACGGCGCTT CGTCCTTCCC AGTTGGGTCG AGTGAAGACC AACAGTGCTT TGTTGGAGCT
    GCATATTATG GCCAAGGGCG AGTGGTTGTA GCCACACATG AAGATTTCCT CTCCAAACCA
    GAACTGAAGA CTTTAATGCT TAATGCCATA TCTTGGCTGG ACGTAGGGCA GAGAAGAATC
    GGAGTCCATG CCGACCTGAG GGGCTTTGCT GAAGTTCTGC AGAAGGAAAA CATCCCCTGT
    CGGGTATCAA GTTTAACCCC CAAGCTGAGT GTCTACTGCT GCCCGTCCTA CAGCGACGCT
    GAAGCAAAGG CCGTCCATCA GTTTGTTGCA GAGGGGGGAG GGCTTCTTAT CGCTGGCCAT
    GCCTGGTGGT GTTCCTATCA GAATTCAGCC TGCAATGTCC TCAACTACCC TGGAAACAAA
    ATACTGAACA GGTTTGGGAT TAGCATTCTG TGTAGAGCCG TCCCTCAGGG CATCTATTGG
    GCAGTCAGTC CCAATGAAGC CAGTCACCAA AATAACTGCC ACAGAGCACT TCATCATTTG
    CAGGTTGCAC TAAAGAGCGG TACCAAACTG CTACAGCCCA TCAGCACCAG GATGCGCAAA
    CTCAGGCAGA ATGTCAGCGC TTTTATGAGG CACTCGGCCT GTTGTGTTCC CAATGTCAGC
    AAGACTTGTC CTGTGAGCGG CTGCTCCGAA GAAGCTTTAC TGCTGAGTTT GGCCCACAAA
    TCCCATAATA TGAGCCACAA GTGCGATGAA TTGAGTATTC TGGAGAAGGA AACAGTCACT
    GTTAATATTG ATGCCACCAA CCCAGGTGCC GACGCTTGGA GAAGCACCGG CCTTTATTTG
    CCCCCCAGAA AGGCAGTGGT TATTGAGTTC CCGGCCTCAG CCGTTCAGCA AGGTTTCCAG
    GTGCAGGTCG GCTGTCACTC AGATGATCCG AGCGCTAAAG AAACGCTTAA TCGAGCGCCG
    GCCGTTGTTC GCAGGATTCA TGTTGATCGC CAGAAGGTTC CAGTGTCCAG TTTCTGGGGA
    GGCCTCCTGT ACATCATTGT GAGGTCTAAC AGCAACTTTG GTACCGTCCC AGTCAGGGTG
    TATGGAGCAG AACCAGCCCC AATCTATATA AAGGGCAAAA CCAGCCTGGA TTCCTGGTTA
    CATTCAATCC GCCAGTTGCC TGCCCCTTGG GCCGAGCTGG TTGCAGAGAA CATTATCCTG
    ACTGTCCCAT CAGATGCCGT TCGCTCACTA AAGAATCCAG AAGCCCTGCT GTCTCTTTGG
    GACAGGATCA TGGCGGCTAT TGCTGACCTT GCAGCCAAAC CCAAGAAATT TCCACGACCT
    GAGCGTATTG TTGCAGATGT GCAGATCTCA GCTGGTTGGA TGCATGCTGG ATACCCCATC
    ATGTGTGACT TGGAATCGGC TAAAGAGCTG ACGGATCTAC ACCATATGGA GAAAGAGGGG
    ATCTGGGGGC CAATTCATGA ACTTGGCCAC AACCAACAGA AAACACAGTG GGAGTTCCCA
    CCCCATACCA CGGAAGCCAC GTGTAACCTC TGGGCTGTGT ATGTGCATGA AACGGTCCTT
    GGGATCCCGC GGGATAGAGC CCACGGCAAC CTTCAGCCCA ACGTCCGGAC GTCTCGTATC
    AAAAGTTACT TGCAGAATGG AGCCAACTTA GAGCAGTGGG ATGGATGGAC AGCGTTGGAG
    ACCTACCTAC AGCTGCAGGA AGGCTTGGGC TGGGACCCAT TCAGGCGCCT ATACAGAGAC
    CCGAGCATCT CCGGCATGAG CAATGAAAAC ACATTCAAGA TGAATCTGTG GGCGGAGAAG
    TTCTCTCGGG CCGCGCAGAC CAATCTGGTT CCGTTCTTTG AGGCCTGGGG ATGGCCCATT
    GACCAATTGA CTCGCAGTAA ACTCTCCGCA TTGCCCGTAT GGGAGAAGGA TCCCATGAAG
    CCCTATGTTA CTGCCAAGAA GTCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53021
    Clone ID Related Accession (Same CDS sequence) XM_031897388.1
    Accession Version XM_031897388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATGCGGT TCCGTGCAAA CTGCTGCTGA CAGGACCCTG TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCGCTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTT AGTACACAGG AGTTTATGGG CTTTTTAAAA
    AATGCTGTAT CTTGGCTGAG GCCAAACCCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    ACATTGTTAG CCCATAATCT ATTTGGGTAC AAAATCCGAA ATACTTCCAC CCTGATTCAG
    GGCCTGGGCG TGTTCTGCAC TAACGGATAT GAGGCCCACC AAGCTGAGGA GATCATCTCA
    TTTGTGCGAG GGGGAGGCGG CCTGCTGATT GGGGCGCAGG CTTGGCACTG GTCCTACAGC
    CATAAAGAGA ATGTTCTGCA CCATTTCCCA GGGAACAAAA TCATATCTGT TTGTGGGGTG
    CACTTCACCA GTGAGTACGG AGAGAAAGGA GATTTCTGGG TGACTGATGA GATGCCGCAG
    GCTCCAGTGT GCACAGAGAA TATGGCGGCT GATGAAGATT ACCAGTCTCT GGTCCGTGGT
    GTTGCGTCTC TGAGTTTCAC TGGGGATGCG GTTCCGTGCA AACTGCTGCT GACAGGACCC
    TGTGCATTCC CGGTGCTGGT GTCGCCCCGA AAAGATGTGC TCATCGCTGC CTCTCGCTAT
    GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT GAGGGTTATC TTAGTACACA GGAGTTTATG
    GGCTTTTTAA AAAATGCTGT ATCTTGGCTG AGGCCAAACC CAGATGTCAT TATTGGGGTT
    CATACTACAT TACCAGTGTT AGCCCATAAT CTATCTGGGT ACAAAGTCCG AAATACTTCC
    ACCCTGATTC AGGGCCTGGG CGTGTTCTGC ACTAACGGAT ATGAGGCCCA CCAAGCTGAG
    GAGATCATCT CATTTGTGCG AGGGGGAGGC GGCCTGCTGA TTGGGGCGCA GGCTTGGCAC
    TGGTCCTACA GCCATAAAGA GAATGTTCTG CACCATTTCC CAGGGAACAA AATCATATCT
    GTTTGTGGGG TGCACTTCAC CAGTGAGTAC GGAGAGAAAG GAGATTTCTT GGTGACTGAA
    GAGATGCCGC AGGCTCCAGT GTGCACAGAT TATGATTTCT CAGTGGGTCA GAAGTTCCTC
    CTGAATGGCG TCTCTCAGCT GGACATCAGT GGGCCGAGTG TCCCCTCAGA CCCCCTCTTG
    CACGGCGCTT CGTCCTTCCC AGTTGGGTCG AGTGAAGACC AACAGTGCTT TGTTGGAGCT
    GCATATTATG GCCAAGGGCG AGTGGTTGTA GCCACACATG AAGATTTCCT CTCCAAACCA
    GAACTGAAGA CTTTAATGCT TAATGCCATA TCTTGGCTGG ACGTAGGGCA GAGAAGAATC
    GGAGTCCATG CCGACCTGAG GGGCTTTGCT GAAGTTCTGC AGAAGGAAAA CATCCCCTGT
    CGGGTATCAA GTTTAACCCC CAAGCTGAGT GTCTACTGCT GCCCGTCCTA CAGCGACGCT
    GAAGCAAAGG CCGTCCATCA GTTTGTTGCA GAGGGGGGAG GGCTTCTTAT CGCTGGCCAT
    GCCTGGTGGT GTTCCTATCA GAATTCAGCC TGCAATGTCC TCAACTACCC TGGAAACAAA
    ATACTGAACA GGTTTGGGAT TAGCATTCTG TGTAGAGCCG TCCCTCAGGG CATCTATTGG
    GCAGTCAGTC CCAATGAAGC CAGTCACCAA AATAACTGCC ACAGAGCACT TCATCATTTG
    CAGGTTGCAC TAAAGAGCGG TACCAAACTG CTACAGCCCA TCAGCACCAG GATGCGCAAA
    CTCAGGCAGA ATGTCAGCGC TTTTATGAGG CACTCGGCCT GTTGTGTTCC CAATGTCAGC
    AAGACTTGTC CTGTGAGCGG CTGCTCCGAA GAAGCTTTAC TGCTGAGTTT GGCCCACAAA
    TCCCATAATA TGAGCCACAA GTGCGATGAA TTGAGTATTC TGGAGAAGGA AACAGTCACT
    GTTAATATTG ATGCCACCAA CCCAGGTGCC GACGCTTGGA GAAGCACCGG CCTTTATTTG
    CCCCCCAGAA AGGCAGTGGT TATTGAGTTC CCGGCCTCAG CCGTTCAGCA AGGTTTCCAG
    GTGCAGGTCG GCTGTCACTC AGATGATCCG AGCGCTAAAG AAACGCTTAA TCGAGCGCCG
    GCCGTTGTTC GCAGGATTCA TGTTGATCGC CAGAAGGTTC CAGTGTCCAG TTTCTGGGGA
    GGCCTCCTGT ACATCATTGT GAGGTCTAAC AGCAACTTTG GTACCGTCCC AGTCAGGGTG
    TATGGAGCAG AACCAGCCCC AATCTATATA AAGGGCAAAA CCAGCCTGGA TTCCTGGTTA
    CATTCAATCC GCCAGTTGCC TGCCCCTTGG GCCGAGCTGG TTGCAGAGAA CATTATCCTG
    ACTGTCCCAT CAGATGCCGT TCGCTCACTA AAGAATCCAG AAGCCCTGCT GTCTCTTTGG
    GACAGGATCA TGGCGGCTAT TGCTGACCTT GCAGCCAAAC CCAAGAAATT TCCACGACCT
    GAGCGTATTG TTGCAGATGT GCAGATCTCA GCTGGTTGGA TGCATGCTGG ATACCCCATC
    ATGTGTGACT TGGAATCGGC TAAAGAGCTG ACGGATCTAC ACCATATGGA GAAAGAGGGG
    ATCTGGGGGC CAATTCATGA ACTTGGCCAC AACCAACAGA AAACACAGTG GGAGTTCCCA
    CCCCATACCA CGGAAGCCAC GTGTAACCTC TGGGCTGTGT ATGTGCATGA AACGGTCCTT
    GGGATCCCGC GGGATAGAGC CCACGGCAAC CTTCAGCCCA ACGTCCGGAC GTCTCGTATC
    AAAAGTTACT TGCAGAATGG AGCCAACTTA GAGCAGTGGG ATGGATGGAC AGCGTTGGAG
    ACCTACCTAC AGCTGCAGGA AGGCTTGGGC TGGGACCCAT TCAGGCGCCT ATACAGAGAC
    CCGAGCATCT CCGGCATGAG CAATGAAAAC ACATTCAAGA TGAATCTGTG GGCGGAGAAG
    TTCTCTCGGG CCGCGCAGAC CAATCTGGTT CCGTTCTTTG AGGCCTGGGG ATGGCCCATT
    GACCAATTGA CTCGCAGTAA ACTCTCCGCA TTGCCCGTAT GGGAGAAGGA TCCCATGAAG
    CCCTATGTTA CTGCCAAGAA GTCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753248.1
    CDS110..4579
    Translation

    Target ORF information:

    RefSeq Version XM_031897388.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATGCGGT TCCGTGCAAA CTGCTGCTGA CAGGACCCTG TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCGCTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTT AGTACACAGG AGTTTATGGG CTTTTTAAAA
    AATGCTGTAT CTTGGCTGAG GCCAAACCCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    ACATTGTTAG CCCATAATCT ATTTGGGTAC AAAATCCGAA ATACTTCCAC CCTGATTCAG
    GGCCTGGGCG TGTTCTGCAC TAACGGATAT GAGGCCCACC AAGCTGAGGA GATCATCTCA
    TTTGTGCGAG GGGGAGGCGG CCTGCTGATT GGGGCGCAGG CTTGGCACTG GTCCTACAGC
    CATAAAGAGA ATGTTCTGCA CCATTTCCCA GGGAACAAAA TCATATCTGT TTGTGGGGTG
    CACTTCACCA GTGAGTACGG AGAGAAAGGA GATTTCTGGG TGACTGATGA GATGCCGCAG
    GCTCCAGTGT GCACAGAGAA TATGGCGGCT GATGAAGATT ACCAGTCTCT GGTCCGTGGT
    GTTGCGTCTC TGAGTTTCAC TGGGGATGCG GTTCCGTGCA AACTGCTGCT GACAGGACCC
    TGTGCATTCC CGGTGCTGGT GTCGCCCCGA AAAGATGTGC TCATCGCTGC CTCTCGCTAT
    GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT GAGGGTTATC TTAGTACACA GGAGTTTATG
    GGCTTTTTAA AAAATGCTGT ATCTTGGCTG AGGCCAAACC CAGATGTCAT TATTGGGGTT
    CATACTACAT TACCAGTGTT AGCCCATAAT CTATCTGGGT ACAAAGTCCG AAATACTTCC
    ACCCTGATTC AGGGCCTGGG CGTGTTCTGC ACTAACGGAT ATGAGGCCCA CCAAGCTGAG
    GAGATCATCT CATTTGTGCG AGGGGGAGGC GGCCTGCTGA TTGGGGCGCA GGCTTGGCAC
    TGGTCCTACA GCCATAAAGA GAATGTTCTG CACCATTTCC CAGGGAACAA AATCATATCT
    GTTTGTGGGG TGCACTTCAC CAGTGAGTAC GGAGAGAAAG GAGATTTCTT GGTGACTGAA
    GAGATGCCGC AGGCTCCAGT GTGCACAGAT TATGATTTCT CAGTGGGTCA GAAGTTCCTC
    CTGAATGGCG TCTCTCAGCT GGACATCAGT GGGCCGAGTG TCCCCTCAGA CCCCCTCTTG
    CACGGCGCTT CGTCCTTCCC AGTTGGGTCG AGTGAAGACC AACAGTGCTT TGTTGGAGCT
    GCATATTATG GCCAAGGGCG AGTGGTTGTA GCCACACATG AAGATTTCCT CTCCAAACCA
    GAACTGAAGA CTTTAATGCT TAATGCCATA TCTTGGCTGG ACGTAGGGCA GAGAAGAATC
    GGAGTCCATG CCGACCTGAG GGGCTTTGCT GAAGTTCTGC AGAAGGAAAA CATCCCCTGT
    CGGGTATCAA GTTTAACCCC CAAGCTGAGT GTCTACTGCT GCCCGTCCTA CAGCGACGCT
    GAAGCAAAGG CCGTCCATCA GTTTGTTGCA GAGGGGGGAG GGCTTCTTAT CGCTGGCCAT
    GCCTGGTGGT GTTCCTATCA GAATTCAGCC TGCAATGTCC TCAACTACCC TGGAAACAAA
    ATACTGAACA GGTTTGGGAT TAGCATTCTG TGTAGAGCCG TCCCTCAGGG CATCTATTGG
    GCAGTCAGTC CCAATGAAGC CAGTCACCAA AATAACTGCC ACAGAGCACT TCATCATTTG
    CAGGTTGCAC TAAAGAGCGG TACCAAACTG CTACAGCCCA TCAGCACCAG GATGCGCAAA
    CTCAGGCAGA ATGTCAGCGC TTTTATGAGG CACTCGGCCT GTTGTGTTCC CAATGTCAGC
    AAGACTTGTC CTGTGAGCGG CTGCTCCGAA GAAGCTTTAC TGCTGAGTTT GGCCCACAAA
    TCCCATAATA TGAGCCACAA GTGCGATGAA TTGAGTATTC TGGAGAAGGA AACAGTCACT
    GTTAATATTG ATGCCACCAA CCCAGGTGCC GACGCTTGGA GAAGCACCGG CCTTTATTTG
    CCCCCCAGAA AGGCAGTGGT TATTGAGTTC CCGGCCTCAG CCGTTCAGCA AGGTTTCCAG
    GTGCAGGTCG GCTGTCACTC AGATGATCCG AGCGCTAAAG AAACGCTTAA TCGAGCGCCG
    GCCGTTGTTC GCAGGATTCA TGTTGATCGC CAGAAGGTTC CAGTGTCCAG TTTCTGGGGA
    GGCCTCCTGT ACATCATTGT GAGGTCTAAC AGCAACTTTG GTACCGTCCC AGTCAGGGTG
    TATGGAGCAG AACCAGCCCC AATCTATATA AAGGGCAAAA CCAGCCTGGA TTCCTGGTTA
    CATTCAATCC GCCAGTTGCC TGCCCCTTGG GCCGAGCTGG TTGCAGAGAA CATTATCCTG
    ACTGTCCCAT CAGATGCCGT TCGCTCACTA AAGAATCCAG AAGCCCTGCT GTCTCTTTGG
    GACAGGATCA TGGCGGCTAT TGCTGACCTT GCAGCCAAAC CCAAGAAATT TCCACGACCT
    GAGCGTATTG TTGCAGATGT GCAGATCTCA GCTGGTTGGA TGCATGCTGG ATACCCCATC
    ATGTGTGACT TGGAATCGGC TAAAGAGCTG ACGGATCTAC ACCATATGGA GAAAGAGGGG
    ATCTGGGGGC CAATTCATGA ACTTGGCCAC AACCAACAGA AAACACAGTG GGAGTTCCCA
    CCCCATACCA CGGAAGCCAC GTGTAACCTC TGGGCTGTGT ATGTGCATGA AACGGTCCTT
    GGGATCCCGC GGGATAGAGC CCACGGCAAC CTTCAGCCCA ACGTCCGGAC GTCTCGTATC
    AAAAGTTACT TGCAGAATGG AGCCAACTTA GAGCAGTGGG ATGGATGGAC AGCGTTGGAG
    ACCTACCTAC AGCTGCAGGA AGGCTTGGGC TGGGACCCAT TCAGGCGCCT ATACAGAGAC
    CCGAGCATCT CCGGCATGAG CAATGAAAAC ACATTCAAGA TGAATCTGTG GGCGGAGAAG
    TTCTCTCGGG CCGCGCAGAC CAATCTGGTT CCGTTCTTTG AGGCCTGGGG ATGGCCCATT
    GACCAATTGA CTCGCAGTAA ACTCTCCGCA TTGCCCGTAT GGGAGAAGGA TCCCATGAAG
    CCCTATGTTA CTGCCAAGAA GTCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53022
    Clone ID Related Accession (Same CDS sequence) XM_031897389.1
    Accession Version XM_031897389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGCTTTT TAAAAAATGC TGTATCTTGG CTGAGGCCAA ACCCAGATGC CATTATTGGG 
    GTTCATACTA CATTACCAGT GTTAGCCCAT AATCTATCTG GGTACAAAGT CCGAAATACT
    TCCACCCTGA TTCAGGGCCT GGGCGTGTTC TGCACTAACG GATATGAGGC CCACCAAGCT
    GAGGAGATCA TCTCATTTGT GCGAGGGGGA GGCGGCCTGC TGATTGGGGC GCAGGCTTGG
    CACTGGTCCT ACAGCCATAA AGAGAATGTT CTGCACCATT TCCCAGGGAA CAAAATCATA
    TCTGTTTGTG GGGTGCACTT CACCAGTGAG TACGGAGAGA AAGGAGATTT CTGGGTGACT
    GAAGAGATGC CGCAGGCTCC AGTGTGCACA GAGAATATGG CGGCTAATGA AGATTACCAG
    TCTCTGGTCC GTGGCGTTGC GTCTCTGAGT TTCACTGGGG ATCCGGTTCC GTGCAAACTG
    CTGCTGAGAA AACCAACTGC ATTCCCGGTG CTGGTGTCGC CCCGAAAAGA TGTGCTCATC
    GCTGCCTCTC ACTATGGGAA AGGGAAAGTA GTGGTTCTGG CCCATGAGGG TTATCTCAAC
    ACAGAGGAGT TTATGGCTTT TCTGAAAAAT GCTGTATCTT GGCTGAGTCC TAATTCAGAT
    GCCGTTATTG GGGTTCATAC TACATTACCA GTGTTAGCCA ATAATCTATC TGGTGCCGGG
    TACAAAGTCC GAAATACTTC CACCCTGATT CAGGGCCTGG GCGTGTTCTG CACTAACGGA
    TATGAGGCCC ACCAAGCTGA GGAGATCATC TCATTTGTGC GAGGGGGAGG CGGCCTGCTG
    ATTGGGGCGC AGGCTTGGCA CTGGTCCTAC AGCCATAAAG AGAATGTTCT GCACCATTTC
    CCAGGGAACA AAATCATATC TGTTTGTGGG GTGCACTTCA CCAGTGAGTA CGGAGAGAAA
    GGAGATTTCT GGGTGACTGA TGAGATGCCG CAGGCTCCAG TGTGCACAGA GAATATGGCG
    GCTGATGAAG ATTACCAGTC TCTGGTCCGT GGCGTTGCGT CTCTGAGTTT CACTGGGGAT
    GCGGTTCCGT GCAAACTGCT GCTGACAGGA CCCTGTGCAT TCCCGGTGCT GGTGTCGCCC
    CGAAAAGATG TGCTCATCGC TGCCTCTCGC TATGGGAAAG GGAAAGTAGT GGTTCTGGCC
    CATGAGGGTT ATCTTAGTAC ACAGGAGTTT ATGGGCTTTT TAAAAAATGC TGTATCTTGG
    CTGAGGCCAA ACCCAGATGC CGTTATTGGG GTTCATACTA CATTAACATT GTTAGCCCAT
    AATCTATTTG GGTACAAAAT CCGAAATACT TCCACCCTGA TTCAGGGCCT GGGCGTGTTC
    TGCACTAACG GATATGAGGC CCACCAAGCT GAGGAGATCA TCTCATTTGT GCGAGGGGGA
    GGCGGCCTGC TGATTGGGGC GCAGGCTTGG CACTGGTCCT ACAGCCATAA AGAGAATGTT
    CTGCACCATT TCCCAGGGAA CAAAATCATA TCTGTTTGTG GGGTGCACTT CACCAGTGAG
    TACGGAGAGA AAGGAGATTT CTGGGTGACT GATGAGATGC CGCAGGCTCC AGTGTGCACA
    GAGAATATGG CGGCTGATGA AGATTACCAG TCTCTGGTCC GTGGTGTTGC GTCTCTGAGT
    TTCACTGGGG ATGCGGTTCC GTGCAAACTG CTGCTGACAG GACCCTGTGC ATTCCCGGTG
    CTGGTGTCGC CCCGAAAAGA TGTGCTCATC GCTGCCTCTC GCTATGGGAA AGGGAAAGTA
    GTGGTTCTGG CCCATGAGGG TTATCTTAGT ACACAGGAGT TTATGGGCTT TTTAAAAAAT
    GCTGTATCTT GGCTGAGGCC AAACCCAGAT GTCATTATTG GGGTTCATAC TACATTACCA
    GTGTTAGCCC ATAATCTATC TGGGTACAAA GTCCGAAATA CTTCCACCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTACAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTTGGTGA CTGAAGAGAT GCCGCAGGCT
    CCAGTGTGCA CAGATTATGA TTTCTCAGTG GGTCAGAAGT TCCTCCTGAA TGGCGTCTCT
    CAGCTGGACA TCAGTGGGCC GAGTGTCCCC TCAGACCCCC TCTTGCACGG CGCTTCGTCC
    TTCCCAGTTG GGTCGAGTGA AGACCAACAG TGCTTTGTTG GAGCTGCATA TTATGGCCAA
    GGGCGAGTGG TTGTAGCCAC ACATGAAGAT TTCCTCTCCA AACCAGAACT GAAGACTTTA
    ATGCTTAATG CCATATCTTG GCTGGACGTA GGGCAGAGAA GAATCGGAGT CCATGCCGAC
    CTGAGGGGCT TTGCTGAAGT TCTGCAGAAG GAAAACATCC CCTGTCGGGT ATCAAGTTTA
    ACCCCCAAGC TGAGTGTCTA CTGCTGCCCG TCCTACAGCG ACGCTGAAGC AAAGGCCGTC
    CATCAGTTTG TTGCAGAGGG GGGAGGGCTT CTTATCGCTG GCCATGCCTG GTGGTGTTCC
    TATCAGAATT CAGCCTGCAA TGTCCTCAAC TACCCTGGAA ACAAAATACT GAACAGGTTT
    GGGATTAGCA TTCTGTGTAG AGCCGTCCCT CAGGGCATCT ATTGGGCAGT CAGTCCCAAT
    GAAGCCAGTC ACCAAAATAA CTGCCACAGA GCACTTCATC ATTTGCAGGT TGCACTAAAG
    AGCGGTACCA AACTGCTACA GCCCATCAGC ACCAGGATGC GCAAACTCAG GCAGAATGTC
    AGCGCTTTTA TGAGGCACTC GGCCTGTTGT GTTCCCAATG TCAGCAAGAC TTGTCCTGTG
    AGCGGCTGCT CCGAAGAAGC TTTACTGCTG AGTTTGGCCC ACAAATCCCA TAATATGAGC
    CACAAGTGCG ATGAATTGAG TATTCTGGAG AAGGAAACAG TCACTGTTAA TATTGATGCC
    ACCAACCCAG GTGCCGACGC TTGGAGAAGC ACCGGCCTTT ATTTGCCCCC CAGAAAGGCA
    GTGGTTATTG AGTTCCCGGC CTCAGCCGTT CAGCAAGGTT TCCAGGTGCA GGTCGGCTGT
    CACTCAGATG ATCCGAGCGC TAAAGAAACG CTTAATCGAG CGCCGGCCGT TGTTCGCAGG
    ATTCATGTTG ATCGCCAGAA GGTTCCAGTG TCCAGTTTCT GGGGAGGCCT CCTGTACATC
    ATTGTGAGGT CTAACAGCAA CTTTGGTACC GTCCCAGTCA GGGTGTATGG AGCAGAACCA
    GCCCCAATCT ATATAAAGGG CAAAACCAGC CTGGATTCCT GGTTACATTC AATCCGCCAG
    TTGCCTGCCC CTTGGGCCGA GCTGGTTGCA GAGAACATTA TCCTGACTGT CCCATCAGAT
    GCCGTTCGCT CACTAAAGAA TCCAGAAGCC CTGCTGTCTC TTTGGGACAG GATCATGGCG
    GCTATTGCTG ACCTTGCAGC CAAACCCAAG AAATTTCCAC GACCTGAGCG TATTGTTGCA
    GATGTGCAGA TCTCAGCTGG TTGGATGCAT GCTGGATACC CCATCATGTG TGACTTGGAA
    TCGGCTAAAG AGCTGACGGA TCTACACCAT ATGGAGAAAG AGGGGATCTG GGGGCCAATT
    CATGAACTTG GCCACAACCA ACAGAAAACA CAGTGGGAGT TCCCACCCCA TACCACGGAA
    GCCACGTGTA ACCTCTGGGC TGTGTATGTG CATGAAACGG TCCTTGGGAT CCCGCGGGAT
    AGAGCCCACG GCAACCTTCA GCCCAACGTC CGGACGTCTC GTATCAAAAG TTACTTGCAG
    AATGGAGCCA ACTTAGAGCA GTGGGATGGA TGGACAGCGT TGGAGACCTA CCTACAGCTG
    CAGGAAGGCT TGGGCTGGGA CCCATTCAGG CGCCTATACA GAGACCCGAG CATCTCCGGC
    ATGAGCAATG AAAACACATT CAAGATGAAT CTGTGGGCGG AGAAGTTCTC TCGGGCCGCG
    CAGACCAATC TGGTTCCGTT CTTTGAGGCC TGGGGATGGC CCATTGACCA ATTGACTCGC
    AGTAAACTCT CCGCATTGCC CGTATGGGAG AAGGATCCCA TGAAGCCCTA TGTTACTGCC
    AAGAAGTCTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753249.1
    CDS359..4633
    Translation

    Target ORF information:

    RefSeq Version XM_031897389.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGCTTTT TAAAAAATGC TGTATCTTGG CTGAGGCCAA ACCCAGATGC CATTATTGGG 
    GTTCATACTA CATTACCAGT GTTAGCCCAT AATCTATCTG GGTACAAAGT CCGAAATACT
    TCCACCCTGA TTCAGGGCCT GGGCGTGTTC TGCACTAACG GATATGAGGC CCACCAAGCT
    GAGGAGATCA TCTCATTTGT GCGAGGGGGA GGCGGCCTGC TGATTGGGGC GCAGGCTTGG
    CACTGGTCCT ACAGCCATAA AGAGAATGTT CTGCACCATT TCCCAGGGAA CAAAATCATA
    TCTGTTTGTG GGGTGCACTT CACCAGTGAG TACGGAGAGA AAGGAGATTT CTGGGTGACT
    GAAGAGATGC CGCAGGCTCC AGTGTGCACA GAGAATATGG CGGCTAATGA AGATTACCAG
    TCTCTGGTCC GTGGCGTTGC GTCTCTGAGT TTCACTGGGG ATCCGGTTCC GTGCAAACTG
    CTGCTGAGAA AACCAACTGC ATTCCCGGTG CTGGTGTCGC CCCGAAAAGA TGTGCTCATC
    GCTGCCTCTC ACTATGGGAA AGGGAAAGTA GTGGTTCTGG CCCATGAGGG TTATCTCAAC
    ACAGAGGAGT TTATGGCTTT TCTGAAAAAT GCTGTATCTT GGCTGAGTCC TAATTCAGAT
    GCCGTTATTG GGGTTCATAC TACATTACCA GTGTTAGCCA ATAATCTATC TGGTGCCGGG
    TACAAAGTCC GAAATACTTC CACCCTGATT CAGGGCCTGG GCGTGTTCTG CACTAACGGA
    TATGAGGCCC ACCAAGCTGA GGAGATCATC TCATTTGTGC GAGGGGGAGG CGGCCTGCTG
    ATTGGGGCGC AGGCTTGGCA CTGGTCCTAC AGCCATAAAG AGAATGTTCT GCACCATTTC
    CCAGGGAACA AAATCATATC TGTTTGTGGG GTGCACTTCA CCAGTGAGTA CGGAGAGAAA
    GGAGATTTCT GGGTGACTGA TGAGATGCCG CAGGCTCCAG TGTGCACAGA GAATATGGCG
    GCTGATGAAG ATTACCAGTC TCTGGTCCGT GGCGTTGCGT CTCTGAGTTT CACTGGGGAT
    GCGGTTCCGT GCAAACTGCT GCTGACAGGA CCCTGTGCAT TCCCGGTGCT GGTGTCGCCC
    CGAAAAGATG TGCTCATCGC TGCCTCTCGC TATGGGAAAG GGAAAGTAGT GGTTCTGGCC
    CATGAGGGTT ATCTTAGTAC ACAGGAGTTT ATGGGCTTTT TAAAAAATGC TGTATCTTGG
    CTGAGGCCAA ACCCAGATGC CGTTATTGGG GTTCATACTA CATTAACATT GTTAGCCCAT
    AATCTATTTG GGTACAAAAT CCGAAATACT TCCACCCTGA TTCAGGGCCT GGGCGTGTTC
    TGCACTAACG GATATGAGGC CCACCAAGCT GAGGAGATCA TCTCATTTGT GCGAGGGGGA
    GGCGGCCTGC TGATTGGGGC GCAGGCTTGG CACTGGTCCT ACAGCCATAA AGAGAATGTT
    CTGCACCATT TCCCAGGGAA CAAAATCATA TCTGTTTGTG GGGTGCACTT CACCAGTGAG
    TACGGAGAGA AAGGAGATTT CTGGGTGACT GATGAGATGC CGCAGGCTCC AGTGTGCACA
    GAGAATATGG CGGCTGATGA AGATTACCAG TCTCTGGTCC GTGGTGTTGC GTCTCTGAGT
    TTCACTGGGG ATGCGGTTCC GTGCAAACTG CTGCTGACAG GACCCTGTGC ATTCCCGGTG
    CTGGTGTCGC CCCGAAAAGA TGTGCTCATC GCTGCCTCTC GCTATGGGAA AGGGAAAGTA
    GTGGTTCTGG CCCATGAGGG TTATCTTAGT ACACAGGAGT TTATGGGCTT TTTAAAAAAT
    GCTGTATCTT GGCTGAGGCC AAACCCAGAT GTCATTATTG GGGTTCATAC TACATTACCA
    GTGTTAGCCC ATAATCTATC TGGGTACAAA GTCCGAAATA CTTCCACCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTACAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTTGGTGA CTGAAGAGAT GCCGCAGGCT
    CCAGTGTGCA CAGATTATGA TTTCTCAGTG GGTCAGAAGT TCCTCCTGAA TGGCGTCTCT
    CAGCTGGACA TCAGTGGGCC GAGTGTCCCC TCAGACCCCC TCTTGCACGG CGCTTCGTCC
    TTCCCAGTTG GGTCGAGTGA AGACCAACAG TGCTTTGTTG GAGCTGCATA TTATGGCCAA
    GGGCGAGTGG TTGTAGCCAC ACATGAAGAT TTCCTCTCCA AACCAGAACT GAAGACTTTA
    ATGCTTAATG CCATATCTTG GCTGGACGTA GGGCAGAGAA GAATCGGAGT CCATGCCGAC
    CTGAGGGGCT TTGCTGAAGT TCTGCAGAAG GAAAACATCC CCTGTCGGGT ATCAAGTTTA
    ACCCCCAAGC TGAGTGTCTA CTGCTGCCCG TCCTACAGCG ACGCTGAAGC AAAGGCCGTC
    CATCAGTTTG TTGCAGAGGG GGGAGGGCTT CTTATCGCTG GCCATGCCTG GTGGTGTTCC
    TATCAGAATT CAGCCTGCAA TGTCCTCAAC TACCCTGGAA ACAAAATACT GAACAGGTTT
    GGGATTAGCA TTCTGTGTAG AGCCGTCCCT CAGGGCATCT ATTGGGCAGT CAGTCCCAAT
    GAAGCCAGTC ACCAAAATAA CTGCCACAGA GCACTTCATC ATTTGCAGGT TGCACTAAAG
    AGCGGTACCA AACTGCTACA GCCCATCAGC ACCAGGATGC GCAAACTCAG GCAGAATGTC
    AGCGCTTTTA TGAGGCACTC GGCCTGTTGT GTTCCCAATG TCAGCAAGAC TTGTCCTGTG
    AGCGGCTGCT CCGAAGAAGC TTTACTGCTG AGTTTGGCCC ACAAATCCCA TAATATGAGC
    CACAAGTGCG ATGAATTGAG TATTCTGGAG AAGGAAACAG TCACTGTTAA TATTGATGCC
    ACCAACCCAG GTGCCGACGC TTGGAGAAGC ACCGGCCTTT ATTTGCCCCC CAGAAAGGCA
    GTGGTTATTG AGTTCCCGGC CTCAGCCGTT CAGCAAGGTT TCCAGGTGCA GGTCGGCTGT
    CACTCAGATG ATCCGAGCGC TAAAGAAACG CTTAATCGAG CGCCGGCCGT TGTTCGCAGG
    ATTCATGTTG ATCGCCAGAA GGTTCCAGTG TCCAGTTTCT GGGGAGGCCT CCTGTACATC
    ATTGTGAGGT CTAACAGCAA CTTTGGTACC GTCCCAGTCA GGGTGTATGG AGCAGAACCA
    GCCCCAATCT ATATAAAGGG CAAAACCAGC CTGGATTCCT GGTTACATTC AATCCGCCAG
    TTGCCTGCCC CTTGGGCCGA GCTGGTTGCA GAGAACATTA TCCTGACTGT CCCATCAGAT
    GCCGTTCGCT CACTAAAGAA TCCAGAAGCC CTGCTGTCTC TTTGGGACAG GATCATGGCG
    GCTATTGCTG ACCTTGCAGC CAAACCCAAG AAATTTCCAC GACCTGAGCG TATTGTTGCA
    GATGTGCAGA TCTCAGCTGG TTGGATGCAT GCTGGATACC CCATCATGTG TGACTTGGAA
    TCGGCTAAAG AGCTGACGGA TCTACACCAT ATGGAGAAAG AGGGGATCTG GGGGCCAATT
    CATGAACTTG GCCACAACCA ACAGAAAACA CAGTGGGAGT TCCCACCCCA TACCACGGAA
    GCCACGTGTA ACCTCTGGGC TGTGTATGTG CATGAAACGG TCCTTGGGAT CCCGCGGGAT
    AGAGCCCACG GCAACCTTCA GCCCAACGTC CGGACGTCTC GTATCAAAAG TTACTTGCAG
    AATGGAGCCA ACTTAGAGCA GTGGGATGGA TGGACAGCGT TGGAGACCTA CCTACAGCTG
    CAGGAAGGCT TGGGCTGGGA CCCATTCAGG CGCCTATACA GAGACCCGAG CATCTCCGGC
    ATGAGCAATG AAAACACATT CAAGATGAAT CTGTGGGCGG AGAAGTTCTC TCGGGCCGCG
    CAGACCAATC TGGTTCCGTT CTTTGAGGCC TGGGGATGGC CCATTGACCA ATTGACTCGC
    AGTAAACTCT CCGCATTGCC CGTATGGGAG AAGGATCCCA TGAAGCCCTA TGTTACTGCC
    AAGAAGTCTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53013
    Clone ID Related Accession (Same CDS sequence) XM_031897379.1
    Accession Version XM_031897379.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATCCGGT TCCGTGCAAA CTGCTGCTGA GAAAACCAAC TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCACTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTC AACACAGAGG AGTTTATGGC TTTTCTGAAA
    AATGCTGTAT CTTGGCTGAG TCCTAATTCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    CCAGTGTTAG CCAATAATCT ATCTGGTGCC GGGTACAAAG TCCGAAATAC TTCCACCCTG
    ATTCAGGGCC TGGGCGTGTT CTGCACTAAC GGATATGAGG CCCACCAAGC TGAGGAGATC
    ATCTCATTTG TGCGAGGGGG AGGCGGCCTG CTGATTGGGG CGCAGGCTTG GCACTGGTCC
    TACAGCCATA AAGAGAATGT TCTGCACCAT TTCCCAGGGA ACAAAATCAT ATCTGTTTGT
    GGGGTGCACT TCACCAGTGA GTACGGAGAG AAAGGAGATT TCTGGGTGAC TGATGAGATG
    CCGCAGGCTC CAGTGTGCAC AGAGAATATG GCGGCTGATG AAGATTACCA GTCTCTGGTC
    CGTGGCGTTG CGTCTCTGAG TTTCACTGGG GATGCGGTTC CGTGCAAACT GCTGCTGACA
    GGACCCTGTG CATTCCCGGT GCTGGTGTCG CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT
    CGCTATGGGA AAGGGAAAGT AGTGGTTCTG GCCCATGAGG GTTATCTTAG TACACAGGAG
    TTTATGGGCT TTTTAAAAAA TGCTGTATCT TGGCTGAGGC CAAACCCAGA TGCCGTTATT
    GGGGTTCATA CTACATTAAC ATTGTTAGCC CATAATCTAT TTGGGTACAA AATCCGAAAT
    ACTTCCACCC TGATTCAGGG CCTGGGCGTG TTCTGCACTA ACGGATATGA GGCCCACCAA
    GCTGAGGAGA TCATCTCATT TGTGCGAGGG GGAGGCGGCC TGCTGATTGG GGCGCAGGCT
    TGGCACTGGT CCTACAGCCA TAAAGAGAAT GTTCTGCACC ATTTCCCAGG GAACAAAATC
    ATATCTGTTT GTGGGGTGCA CTTCACCAGT GAGTACGGAG AGAAAGGAGA TTTCTGGGTG
    ACTGATGAGA TGCCGCAGGC TCCAGTGTGC ACAGAGAATA TGGCGGCTGA TGAAGATTAC
    CAGTCTCTGG TCCGTGGTGT TGCGTCTCTG AGTTTCACTG GGGATGCGGT TCCGTGCAAA
    CTGCTGCTGA CAGGACCCTG TGCATTCCCG GTGCTGGTGT CGCCCCGAAA AGATGTGCTC
    ATCGCTGCCT CTCGCTATGG GAAAGGGAAA GTAGTGGTTC TGGCCCATGA GGGTTATCTT
    AGTACACAGG AGTTTATGGG CTTTTTAAAA AATGCTGTAT CTTGGCTGAG GCCAAACCCA
    GATGTCATTA TTGGGGTTCA TACTACATTA CCAGTGTTAG CCCATAATCT ATCTGGGTAC
    AAAGTCCGAA ATACTTCCAC CCTGATTCAG GGCCTGGGCG TGTTCTGCAC TAACGGATAT
    GAGGCCCACC AAGCTGAGGA GATCATCTCA TTTGTGCGAG GGGGAGGCGG CCTGCTGATT
    GGGGCGCAGG CTTGGCACTG GTCCTACAGC CATAAAGAGA ATGTTCTGCA CCATTTCCCA
    GGGAACAAAA TCATATCTGT TTGTGGGGTG CACTTCACCA GTGAGTACGG AGAGAAAGGA
    GATTTCTTGG TGACTGAAGA GATGCCGCAG GCTCCAGTGT GCACAGATTA TGATTTCTCA
    GTGGGTCAGA AGTTCCTCCT GAATGGCGTC TCTCAGCTGG ACATCAGTGG GCCGAGTGTC
    CCCTCAGACC CCCTCTTGCA CGGCGCTTCG TCCTTCCCAG TTGGGTCGAG TGAAGACCAA
    CAGTGCTTTG TTGGAGCTGC ATATTATGGC CAAGGGCGAG TGGTTGTAGC CACACATGAA
    GATTTCCTCT CCAAACCAGA ACTGAAGACT TTAATGCTTA ATGCCATATC TTGGCTGGAC
    GTAGGGCAGA GAAGAATCGG AGTCCATGCC GACCTGAGGG GCTTTGCTGA AGTTCTGCAG
    AAGGAAAACA TCCCCTGTCG GGTATCAAGT TTAACCCCCA AGCTGAGTGT CTACTGCTGC
    CCGTCCTACA GCGACGCTGA AGCAAAGGCC GTCCATCAGT TTGTTGCAGA GGGGGGAGGG
    CTTCTTATCG CTGGCCATGC CTGGTGGTGT TCCTATCAGA ATTCAGCCTG CAATGTCCTC
    AACTACCCTG GAAACAAAAT ACTGAACAGG TTTGGGATTA GCATTCTGTG TAGAGCCGTC
    CCTCAGGGCA TCTATTGGGC AGTCAGTCCC AATGAAGCCA GTCACCAAAA TAACTGCCAC
    AGAGCACTTC ATCATTTGCA GGTTGCACTA AAGAGCGGTA CCAAACTGCT ACAGCCCATC
    AGCACCAGGA TGCGCAAACT CAGGCAGAAT GTCAGCGCTT TTATGAGGCA CTCGGCCTGT
    TGTGTTCCCA ATGTCAGCAA GACTTGTCCT GTGAGCGGCT GCTCCGAAGA AGCTTTACTG
    CTGAGTTTGG CCCACAAATC CCATAATATG AGCCACAAGT GCGATGAATT GAGTATTCTG
    GAGAAGGAAA CAGTCACTGT TAATATTGAT GCCACCAACC CAGGTGCCGA CGCTTGGAGA
    AGCACCGGCC TTTATTTGCC CCCCAGAAAG GCAGTGGTTA TTGAGTTCCC GGCCTCAGCC
    GTTCAGCAAG GTTTCCAGGT GCAGGTCGGC TGTCACTCAG ATGATCCGAG CGCTAAAGAA
    ACGCTTAATC GAGCGCCGGC CGTTGTTCGC AGGATTCATG TTGATCGCCA GAAGGTTCCA
    GTGTCCAGTT TCTGGGGAGG CCTCCTGTAC ATCATTGTGA GGTCTAACAG CAACTTTGGT
    ACCGTCCCAG TCAGGGTGTA TGGAGCAGAA CCAGCCCCAA TCTATATAAA GGGCAAAACC
    AGCCTGGATT CCTGGTTACA TTCAATCCGC CAGTTGCCTG CCCCTTGGGC CGAGCTGGTT
    GCAGAGAACA TTATCCTGAC TGTCCCATCA GATGCCGTTC GCTCACTAAA GAATCCAGAA
    GCCCTGCTGT CTCTTTGGGA CAGGATCATG GCGGCTATTG CTGACCTTGC AGCCAAACCC
    AAGAAATTTC CACGACCTGA GCGTATTGTT GCAGATGTGC AGATCTCAGC TGGTTGGATG
    CATGCTGGAT ACCCCATCAT GTGTGACTTG GAATCGGCTA AAGAGCTGAC GGATCTACAC
    CATATGGAGA AAGAGGGGAT CTGGGGGCCA ATTCATGAAC TTGGCCACAA CCAACAGAAA
    ACACAGTGGG AGTTCCCACC CCATACCACG GAAGCCACGT GTAACCTCTG GGCTGTGTAT
    GTGCATGAAA CGGTCCTTGG GATCCCGCGG GATAGAGCCC ACGGCAACCT TCAGCCCAAC
    GTCCGGACGT CTCGTATCAA AAGTTACTTG CAGAATGGAG CCAACTTAGA GCAGTGGGAT
    GGATGGACAG CGTTGGAGAC CTACCTACAG CTGCAGGAAG GCTTGGGCTG GGACCCATTC
    AGGCGCCTAT ACAGAGACCC GAGCATCTCC GGCATGAGCA ATGAAAACAC ATTCAAGATG
    AATCTGTGGG CGGAGAAGTT CTCTCGGGCC GCGCAGACCA ATCTGGTTCC GTTCTTTGAG
    GCCTGGGGAT GGCCCATTGA CCAATTGACT CGCAGTAAAC TCTCCGCATT GCCCGTATGG
    GAGAAGGATC CCATGAAGCC CTATGTTACT GCCAAGAAGT CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753239.1
    CDS108..5195
    Translation

    Target ORF information:

    RefSeq Version XM_031897379.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897379.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGAGAATAT GGCGGCTAAT GAAGATTACC AGTCTCTGGT CCGTGGCGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAA GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAATA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGCCGTTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTGGGT GACTGAAGAG
    ATGCCGCAGG TTGCAGTGTG CACAGATTAC CAGTCTCTGG TCCGTGGCGT TGCGTCTCTG
    AGTTTCACTG GGGATCCGGT TCCGTGCAAA CTGCTGCTGA GAAAACCAAC TGCATTCCCG
    GTGCTGGTGT CGCCCCGAAA AGATGTGCTC ATCGCTGCCT CTCACTATGG GAAAGGGAAA
    GTAGTGGTTC TGGCCCATGA GGGTTATCTC AACACAGAGG AGTTTATGGC TTTTCTGAAA
    AATGCTGTAT CTTGGCTGAG TCCTAATTCA GATGCCGTTA TTGGGGTTCA TACTACATTA
    CCAGTGTTAG CCAATAATCT ATCTGGTGCC GGGTACAAAG TCCGAAATAC TTCCACCCTG
    ATTCAGGGCC TGGGCGTGTT CTGCACTAAC GGATATGAGG CCCACCAAGC TGAGGAGATC
    ATCTCATTTG TGCGAGGGGG AGGCGGCCTG CTGATTGGGG CGCAGGCTTG GCACTGGTCC
    TACAGCCATA AAGAGAATGT TCTGCACCAT TTCCCAGGGA ACAAAATCAT ATCTGTTTGT
    GGGGTGCACT TCACCAGTGA GTACGGAGAG AAAGGAGATT TCTGGGTGAC TGATGAGATG
    CCGCAGGCTC CAGTGTGCAC AGAGAATATG GCGGCTGATG AAGATTACCA GTCTCTGGTC
    CGTGGCGTTG CGTCTCTGAG TTTCACTGGG GATGCGGTTC CGTGCAAACT GCTGCTGACA
    GGACCCTGTG CATTCCCGGT GCTGGTGTCG CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT
    CGCTATGGGA AAGGGAAAGT AGTGGTTCTG GCCCATGAGG GTTATCTTAG TACACAGGAG
    TTTATGGGCT TTTTAAAAAA TGCTGTATCT TGGCTGAGGC CAAACCCAGA TGCCGTTATT
    GGGGTTCATA CTACATTAAC ATTGTTAGCC CATAATCTAT TTGGGTACAA AATCCGAAAT
    ACTTCCACCC TGATTCAGGG CCTGGGCGTG TTCTGCACTA ACGGATATGA GGCCCACCAA
    GCTGAGGAGA TCATCTCATT TGTGCGAGGG GGAGGCGGCC TGCTGATTGG GGCGCAGGCT
    TGGCACTGGT CCTACAGCCA TAAAGAGAAT GTTCTGCACC ATTTCCCAGG GAACAAAATC
    ATATCTGTTT GTGGGGTGCA CTTCACCAGT GAGTACGGAG AGAAAGGAGA TTTCTGGGTG
    ACTGATGAGA TGCCGCAGGC TCCAGTGTGC ACAGAGAATA TGGCGGCTGA TGAAGATTAC
    CAGTCTCTGG TCCGTGGTGT TGCGTCTCTG AGTTTCACTG GGGATGCGGT TCCGTGCAAA
    CTGCTGCTGA CAGGACCCTG TGCATTCCCG GTGCTGGTGT CGCCCCGAAA AGATGTGCTC
    ATCGCTGCCT CTCGCTATGG GAAAGGGAAA GTAGTGGTTC TGGCCCATGA GGGTTATCTT
    AGTACACAGG AGTTTATGGG CTTTTTAAAA AATGCTGTAT CTTGGCTGAG GCCAAACCCA
    GATGTCATTA TTGGGGTTCA TACTACATTA CCAGTGTTAG CCCATAATCT ATCTGGGTAC
    AAAGTCCGAA ATACTTCCAC CCTGATTCAG GGCCTGGGCG TGTTCTGCAC TAACGGATAT
    GAGGCCCACC AAGCTGAGGA GATCATCTCA TTTGTGCGAG GGGGAGGCGG CCTGCTGATT
    GGGGCGCAGG CTTGGCACTG GTCCTACAGC CATAAAGAGA ATGTTCTGCA CCATTTCCCA
    GGGAACAAAA TCATATCTGT TTGTGGGGTG CACTTCACCA GTGAGTACGG AGAGAAAGGA
    GATTTCTTGG TGACTGAAGA GATGCCGCAG GCTCCAGTGT GCACAGATTA TGATTTCTCA
    GTGGGTCAGA AGTTCCTCCT GAATGGCGTC TCTCAGCTGG ACATCAGTGG GCCGAGTGTC
    CCCTCAGACC CCCTCTTGCA CGGCGCTTCG TCCTTCCCAG TTGGGTCGAG TGAAGACCAA
    CAGTGCTTTG TTGGAGCTGC ATATTATGGC CAAGGGCGAG TGGTTGTAGC CACACATGAA
    GATTTCCTCT CCAAACCAGA ACTGAAGACT TTAATGCTTA ATGCCATATC TTGGCTGGAC
    GTAGGGCAGA GAAGAATCGG AGTCCATGCC GACCTGAGGG GCTTTGCTGA AGTTCTGCAG
    AAGGAAAACA TCCCCTGTCG GGTATCAAGT TTAACCCCCA AGCTGAGTGT CTACTGCTGC
    CCGTCCTACA GCGACGCTGA AGCAAAGGCC GTCCATCAGT TTGTTGCAGA GGGGGGAGGG
    CTTCTTATCG CTGGCCATGC CTGGTGGTGT TCCTATCAGA ATTCAGCCTG CAATGTCCTC
    AACTACCCTG GAAACAAAAT ACTGAACAGG TTTGGGATTA GCATTCTGTG TAGAGCCGTC
    CCTCAGGGCA TCTATTGGGC AGTCAGTCCC AATGAAGCCA GTCACCAAAA TAACTGCCAC
    AGAGCACTTC ATCATTTGCA GGTTGCACTA AAGAGCGGTA CCAAACTGCT ACAGCCCATC
    AGCACCAGGA TGCGCAAACT CAGGCAGAAT GTCAGCGCTT TTATGAGGCA CTCGGCCTGT
    TGTGTTCCCA ATGTCAGCAA GACTTGTCCT GTGAGCGGCT GCTCCGAAGA AGCTTTACTG
    CTGAGTTTGG CCCACAAATC CCATAATATG AGCCACAAGT GCGATGAATT GAGTATTCTG
    GAGAAGGAAA CAGTCACTGT TAATATTGAT GCCACCAACC CAGGTGCCGA CGCTTGGAGA
    AGCACCGGCC TTTATTTGCC CCCCAGAAAG GCAGTGGTTA TTGAGTTCCC GGCCTCAGCC
    GTTCAGCAAG GTTTCCAGGT GCAGGTCGGC TGTCACTCAG ATGATCCGAG CGCTAAAGAA
    ACGCTTAATC GAGCGCCGGC CGTTGTTCGC AGGATTCATG TTGATCGCCA GAAGGTTCCA
    GTGTCCAGTT TCTGGGGAGG CCTCCTGTAC ATCATTGTGA GGTCTAACAG CAACTTTGGT
    ACCGTCCCAG TCAGGGTGTA TGGAGCAGAA CCAGCCCCAA TCTATATAAA GGGCAAAACC
    AGCCTGGATT CCTGGTTACA TTCAATCCGC CAGTTGCCTG CCCCTTGGGC CGAGCTGGTT
    GCAGAGAACA TTATCCTGAC TGTCCCATCA GATGCCGTTC GCTCACTAAA GAATCCAGAA
    GCCCTGCTGT CTCTTTGGGA CAGGATCATG GCGGCTATTG CTGACCTTGC AGCCAAACCC
    AAGAAATTTC CACGACCTGA GCGTATTGTT GCAGATGTGC AGATCTCAGC TGGTTGGATG
    CATGCTGGAT ACCCCATCAT GTGTGACTTG GAATCGGCTA AAGAGCTGAC GGATCTACAC
    CATATGGAGA AAGAGGGGAT CTGGGGGCCA ATTCATGAAC TTGGCCACAA CCAACAGAAA
    ACACAGTGGG AGTTCCCACC CCATACCACG GAAGCCACGT GTAACCTCTG GGCTGTGTAT
    GTGCATGAAA CGGTCCTTGG GATCCCGCGG GATAGAGCCC ACGGCAACCT TCAGCCCAAC
    GTCCGGACGT CTCGTATCAA AAGTTACTTG CAGAATGGAG CCAACTTAGA GCAGTGGGAT
    GGATGGACAG CGTTGGAGAC CTACCTACAG CTGCAGGAAG GCTTGGGCTG GGACCCATTC
    AGGCGCCTAT ACAGAGACCC GAGCATCTCC GGCATGAGCA ATGAAAACAC ATTCAAGATG
    AATCTGTGGG CGGAGAAGTT CTCTCGGGCC GCGCAGACCA ATCTGGTTCC GTTCTTTGAG
    GCCTGGGGAT GGCCCATTGA CCAATTGACT CGCAGTAAAC TCTCCGCATT GCCCGTATGG
    GAGAAGGATC CCATGAAGCC CTATGTTACT GCCAAGAAGT CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53014
    Clone ID Related Accession (Same CDS sequence) XM_031897380.1
    Accession Version XM_031897380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGATTACCA GTCTCTGGTC CGTGGCGTTG CGTCTCTGAG TTTCACTGGG
    GATGCGGTTC CGTGCAAACT GCTGCTGACA GGACCCTGTG CATTCCCGGT GCTGGTGTCG
    CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT CGCTATGGGA AAGGGAAAGT AGTGGTTCTG
    GCCCATGAAG GTTATCTTAG TACACAGGAG TTTATGGGCT TTTTAAATAA TGCTGTATCT
    TGGCTGAGGC CAAACCCAGA TGCCGTTATT GGGGTTCATA CTACATTACC AGTGTTAGCC
    CATAATCTAT CTGGGTACAA AGTCCGAAAT ACTTCCACCC TGATTCAGGG CCTGGGCGTG
    TTCTGCACTA ACGGATATGA GGCCCACCAA GCTGAGGAGA TCATCTCATT TGTGCGAGGG
    GGAGGCGGCC TGCTGATTGG GGCGCAGGCT TGGCACTGGT CCTACAGCCA TAAAGAGAAT
    GTTCTGCACC ATTTCCCAGG GAACAAAATC ATATCTGTTT GTGGGGTGCA CTTCACCAGT
    GAGTACGGAG AGAAAGGAGA TTTCTGGGTG ACTGAAGAGA TGCCGCAGGT TGCAGTGTGC
    ACAGATTACC AGTCTCTGGT CCGTGGCGTT GCGTCTCTGA GTTTCACTGG GGATCCGGTT
    CCGTGCAAAC TGCTGCTGAG AAAACCAACT GCATTCCCGG TGCTGGTGTC GCCCCGAAAA
    GATGTGCTCA TCGCTGCCTC TCACTATGGG AAAGGGAAAG TAGTGGTTCT GGCCCATGAG
    GGTTATCTCA ACACAGAGGA GTTTATGGCT TTTCTGAAAA ATGCTGTATC TTGGCTGAGT
    CCTAATTCAG ATGCCGTTAT TGGGGTTCAT ACTACATTAC CAGTGTTAGC CAATAATCTA
    TCTGGTGCCG GGTACAAAGT CCGAAATACT TCCACCCTGA TTCAGGGCCT GGGCGTGTTC
    TGCACTAACG GATATGAGGC CCACCAAGCT GAGGAGATCA TCTCATTTGT GCGAGGGGGA
    GGCGGCCTGC TGATTGGGGC GCAGGCTTGG CACTGGTCCT ACAGCCATAA AGAGAATGTT
    CTGCACCATT TCCCAGGGAA CAAAATCATA TCTGTTTGTG GGGTGCACTT CACCAGTGAG
    TACGGAGAGA AAGGAGATTT CTGGGTGACT GATGAGATGC CGCAGGCTCC AGTGTGCACA
    GAGAATATGG CGGCTGATGA AGATTACCAG TCTCTGGTCC GTGGCGTTGC GTCTCTGAGT
    TTCACTGGGG ATGCGGTTCC GTGCAAACTG CTGCTGACAG GACCCTGTGC ATTCCCGGTG
    CTGGTGTCGC CCCGAAAAGA TGTGCTCATC GCTGCCTCTC GCTATGGGAA AGGGAAAGTA
    GTGGTTCTGG CCCATGAGGG TTATCTTAGT ACACAGGAGT TTATGGGCTT TTTAAAAAAT
    GCTGTATCTT GGCTGAGGCC AAACCCAGAT GCCGTTATTG GGGTTCATAC TACATTAACA
    TTGTTAGCCC ATAATCTATT TGGGTACAAA ATCCGAAATA CTTCCACCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTACAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGATGAGAT GCCGCAGGCT
    CCAGTGTGCA CAGAGAATAT GGCGGCTGAT GAAGATTACC AGTCTCTGGT CCGTGGTGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAG GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAAAA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGTCATTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTTGGT GACTGAAGAG
    ATGCCGCAGG CTCCAGTGTG CACAGATTAT GATTTCTCAG TGGGTCAGAA GTTCCTCCTG
    AATGGCGTCT CTCAGCTGGA CATCAGTGGG CCGAGTGTCC CCTCAGACCC CCTCTTGCAC
    GGCGCTTCGT CCTTCCCAGT TGGGTCGAGT GAAGACCAAC AGTGCTTTGT TGGAGCTGCA
    TATTATGGCC AAGGGCGAGT GGTTGTAGCC ACACATGAAG ATTTCCTCTC CAAACCAGAA
    CTGAAGACTT TAATGCTTAA TGCCATATCT TGGCTGGACG TAGGGCAGAG AAGAATCGGA
    GTCCATGCCG ACCTGAGGGG CTTTGCTGAA GTTCTGCAGA AGGAAAACAT CCCCTGTCGG
    GTATCAAGTT TAACCCCCAA GCTGAGTGTC TACTGCTGCC CGTCCTACAG CGACGCTGAA
    GCAAAGGCCG TCCATCAGTT TGTTGCAGAG GGGGGAGGGC TTCTTATCGC TGGCCATGCC
    TGGTGGTGTT CCTATCAGAA TTCAGCCTGC AATGTCCTCA ACTACCCTGG AAACAAAATA
    CTGAACAGGT TTGGGATTAG CATTCTGTGT AGAGCCGTCC CTCAGGGCAT CTATTGGGCA
    GTCAGTCCCA ATGAAGCCAG TCACCAAAAT AACTGCCACA GAGCACTTCA TCATTTGCAG
    GTTGCACTAA AGAGCGGTAC CAAACTGCTA CAGCCCATCA GCACCAGGAT GCGCAAACTC
    AGGCAGAATG TCAGCGCTTT TATGAGGCAC TCGGCCTGTT GTGTTCCCAA TGTCAGCAAG
    ACTTGTCCTG TGAGCGGCTG CTCCGAAGAA GCTTTACTGC TGAGTTTGGC CCACAAATCC
    CATAATATGA GCCACAAGTG CGATGAATTG AGTATTCTGG AGAAGGAAAC AGTCACTGTT
    AATATTGATG CCACCAACCC AGGTGCCGAC GCTTGGAGAA GCACCGGCCT TTATTTGCCC
    CCCAGAAAGG CAGTGGTTAT TGAGTTCCCG GCCTCAGCCG TTCAGCAAGG TTTCCAGGTG
    CAGGTCGGCT GTCACTCAGA TGATCCGAGC GCTAAAGAAA CGCTTAATCG AGCGCCGGCC
    GTTGTTCGCA GGATTCATGT TGATCGCCAG AAGGTTCCAG TGTCCAGTTT CTGGGGAGGC
    CTCCTGTACA TCATTGTGAG GTCTAACAGC AACTTTGGTA CCGTCCCAGT CAGGGTGTAT
    GGAGCAGAAC CAGCCCCAAT CTATATAAAG GGCAAAACCA GCCTGGATTC CTGGTTACAT
    TCAATCCGCC AGTTGCCTGC CCCTTGGGCC GAGCTGGTTG CAGAGAACAT TATCCTGACT
    GTCCCATCAG ATGCCGTTCG CTCACTAAAG AATCCAGAAG CCCTGCTGTC TCTTTGGGAC
    AGGATCATGG CGGCTATTGC TGACCTTGCA GCCAAACCCA AGAAATTTCC ACGACCTGAG
    CGTATTGTTG CAGATGTGCA GATCTCAGCT GGTTGGATGC ATGCTGGATA CCCCATCATG
    TGTGACTTGG AATCGGCTAA AGAGCTGACG GATCTACACC ATATGGAGAA AGAGGGGATC
    TGGGGGCCAA TTCATGAACT TGGCCACAAC CAACAGAAAA CACAGTGGGA GTTCCCACCC
    CATACCACGG AAGCCACGTG TAACCTCTGG GCTGTGTATG TGCATGAAAC GGTCCTTGGG
    ATCCCGCGGG ATAGAGCCCA CGGCAACCTT CAGCCCAACG TCCGGACGTC TCGTATCAAA
    AGTTACTTGC AGAATGGAGC CAACTTAGAG CAGTGGGATG GATGGACAGC GTTGGAGACC
    TACCTACAGC TGCAGGAAGG CTTGGGCTGG GACCCATTCA GGCGCCTATA CAGAGACCCG
    AGCATCTCCG GCATGAGCAA TGAAAACACA TTCAAGATGA ATCTGTGGGC GGAGAAGTTC
    TCTCGGGCCG CGCAGACCAA TCTGGTTCCG TTCTTTGAGG CCTGGGGATG GCCCATTGAC
    CAATTGACTC GCAGTAAACT CTCCGCATTG CCCGTATGGG AGAAGGATCC CATGAAGCCC
    TATGTTACTG CCAAGAAGTC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753240.1
    CDS108..5174
    Translation

    Target ORF information:

    RefSeq Version XM_031897380.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGGCTG ATGAAGATTA CCAGTCTCTG GTCCGTGGCG TTGCTTCTCT GAGTTTCACT 
    GGGGATGCGG TTCCGTGCAA ACTGCTGCTG ACAGGACCCT GTGCATTCCC GGTGCTGGTG
    TCGCCCCGAA AAGATGTGCT CATCGCTGCC TCTCGCTATG GGAAAGGGAA AGTAGTGGTT
    CTGGCCCATG AGGGTTATCT CAACACAGAG GAGTTTATGG CTTTTCTGAA AAATGCTGTA
    TCTTGGCTGA GTCCTAATTC AGATGCCGTT ATTGGGGTTC ATACTACATT ACCAGTGTTA
    GCCAATAATC TATCTGGTGC CGGGTACAAA GTCCGAAAAA CTTCCTCCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTGCAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT
    GCAGTGTGCA CAGATTACCA GTCTCTGGTC CGTGGCGTTG CGTCTCTGAG TTTCACTGGG
    GATGCGGTTC CGTGCAAACT GCTGCTGACA GGACCCTGTG CATTCCCGGT GCTGGTGTCG
    CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT CGCTATGGGA AAGGGAAAGT AGTGGTTCTG
    GCCCATGAAG GTTATCTTAG TACACAGGAG TTTATGGGCT TTTTAAATAA TGCTGTATCT
    TGGCTGAGGC CAAACCCAGA TGCCGTTATT GGGGTTCATA CTACATTACC AGTGTTAGCC
    CATAATCTAT CTGGGTACAA AGTCCGAAAT ACTTCCACCC TGATTCAGGG CCTGGGCGTG
    TTCTGCACTA ACGGATATGA GGCCCACCAA GCTGAGGAGA TCATCTCATT TGTGCGAGGG
    GGAGGCGGCC TGCTGATTGG GGCGCAGGCT TGGCACTGGT CCTACAGCCA TAAAGAGAAT
    GTTCTGCACC ATTTCCCAGG GAACAAAATC ATATCTGTTT GTGGGGTGCA CTTCACCAGT
    GAGTACGGAG AGAAAGGAGA TTTCTGGGTG ACTGAAGAGA TGCCGCAGGT TGCAGTGTGC
    ACAGATTACC AGTCTCTGGT CCGTGGCGTT GCGTCTCTGA GTTTCACTGG GGATCCGGTT
    CCGTGCAAAC TGCTGCTGAG AAAACCAACT GCATTCCCGG TGCTGGTGTC GCCCCGAAAA
    GATGTGCTCA TCGCTGCCTC TCACTATGGG AAAGGGAAAG TAGTGGTTCT GGCCCATGAG
    GGTTATCTCA ACACAGAGGA GTTTATGGCT TTTCTGAAAA ATGCTGTATC TTGGCTGAGT
    CCTAATTCAG ATGCCGTTAT TGGGGTTCAT ACTACATTAC CAGTGTTAGC CAATAATCTA
    TCTGGTGCCG GGTACAAAGT CCGAAATACT TCCACCCTGA TTCAGGGCCT GGGCGTGTTC
    TGCACTAACG GATATGAGGC CCACCAAGCT GAGGAGATCA TCTCATTTGT GCGAGGGGGA
    GGCGGCCTGC TGATTGGGGC GCAGGCTTGG CACTGGTCCT ACAGCCATAA AGAGAATGTT
    CTGCACCATT TCCCAGGGAA CAAAATCATA TCTGTTTGTG GGGTGCACTT CACCAGTGAG
    TACGGAGAGA AAGGAGATTT CTGGGTGACT GATGAGATGC CGCAGGCTCC AGTGTGCACA
    GAGAATATGG CGGCTGATGA AGATTACCAG TCTCTGGTCC GTGGCGTTGC GTCTCTGAGT
    TTCACTGGGG ATGCGGTTCC GTGCAAACTG CTGCTGACAG GACCCTGTGC ATTCCCGGTG
    CTGGTGTCGC CCCGAAAAGA TGTGCTCATC GCTGCCTCTC GCTATGGGAA AGGGAAAGTA
    GTGGTTCTGG CCCATGAGGG TTATCTTAGT ACACAGGAGT TTATGGGCTT TTTAAAAAAT
    GCTGTATCTT GGCTGAGGCC AAACCCAGAT GCCGTTATTG GGGTTCATAC TACATTAACA
    TTGTTAGCCC ATAATCTATT TGGGTACAAA ATCCGAAATA CTTCCACCCT GATTCAGGGC
    CTGGGCGTGT TCTGCACTAA CGGATATGAG GCCCACCAAG CTGAGGAGAT CATCTCATTT
    GTGCGAGGGG GAGGCGGCCT GCTGATTGGG GCGCAGGCTT GGCACTGGTC CTACAGCCAT
    AAAGAGAATG TTCTGCACCA TTTCCCAGGG AACAAAATCA TATCTGTTTG TGGGGTGCAC
    TTCACCAGTG AGTACGGAGA GAAAGGAGAT TTCTGGGTGA CTGATGAGAT GCCGCAGGCT
    CCAGTGTGCA CAGAGAATAT GGCGGCTGAT GAAGATTACC AGTCTCTGGT CCGTGGTGTT
    GCGTCTCTGA GTTTCACTGG GGATGCGGTT CCGTGCAAAC TGCTGCTGAC AGGACCCTGT
    GCATTCCCGG TGCTGGTGTC GCCCCGAAAA GATGTGCTCA TCGCTGCCTC TCGCTATGGG
    AAAGGGAAAG TAGTGGTTCT GGCCCATGAG GGTTATCTTA GTACACAGGA GTTTATGGGC
    TTTTTAAAAA ATGCTGTATC TTGGCTGAGG CCAAACCCAG ATGTCATTAT TGGGGTTCAT
    ACTACATTAC CAGTGTTAGC CCATAATCTA TCTGGGTACA AAGTCCGAAA TACTTCCACC
    CTGATTCAGG GCCTGGGCGT GTTCTGCACT AACGGATATG AGGCCCACCA AGCTGAGGAG
    ATCATCTCAT TTGTGCGAGG GGGAGGCGGC CTGCTGATTG GGGCGCAGGC TTGGCACTGG
    TCCTACAGCC ATAAAGAGAA TGTTCTGCAC CATTTCCCAG GGAACAAAAT CATATCTGTT
    TGTGGGGTGC ACTTCACCAG TGAGTACGGA GAGAAAGGAG ATTTCTTGGT GACTGAAGAG
    ATGCCGCAGG CTCCAGTGTG CACAGATTAT GATTTCTCAG TGGGTCAGAA GTTCCTCCTG
    AATGGCGTCT CTCAGCTGGA CATCAGTGGG CCGAGTGTCC CCTCAGACCC CCTCTTGCAC
    GGCGCTTCGT CCTTCCCAGT TGGGTCGAGT GAAGACCAAC AGTGCTTTGT TGGAGCTGCA
    TATTATGGCC AAGGGCGAGT GGTTGTAGCC ACACATGAAG ATTTCCTCTC CAAACCAGAA
    CTGAAGACTT TAATGCTTAA TGCCATATCT TGGCTGGACG TAGGGCAGAG AAGAATCGGA
    GTCCATGCCG ACCTGAGGGG CTTTGCTGAA GTTCTGCAGA AGGAAAACAT CCCCTGTCGG
    GTATCAAGTT TAACCCCCAA GCTGAGTGTC TACTGCTGCC CGTCCTACAG CGACGCTGAA
    GCAAAGGCCG TCCATCAGTT TGTTGCAGAG GGGGGAGGGC TTCTTATCGC TGGCCATGCC
    TGGTGGTGTT CCTATCAGAA TTCAGCCTGC AATGTCCTCA ACTACCCTGG AAACAAAATA
    CTGAACAGGT TTGGGATTAG CATTCTGTGT AGAGCCGTCC CTCAGGGCAT CTATTGGGCA
    GTCAGTCCCA ATGAAGCCAG TCACCAAAAT AACTGCCACA GAGCACTTCA TCATTTGCAG
    GTTGCACTAA AGAGCGGTAC CAAACTGCTA CAGCCCATCA GCACCAGGAT GCGCAAACTC
    AGGCAGAATG TCAGCGCTTT TATGAGGCAC TCGGCCTGTT GTGTTCCCAA TGTCAGCAAG
    ACTTGTCCTG TGAGCGGCTG CTCCGAAGAA GCTTTACTGC TGAGTTTGGC CCACAAATCC
    CATAATATGA GCCACAAGTG CGATGAATTG AGTATTCTGG AGAAGGAAAC AGTCACTGTT
    AATATTGATG CCACCAACCC AGGTGCCGAC GCTTGGAGAA GCACCGGCCT TTATTTGCCC
    CCCAGAAAGG CAGTGGTTAT TGAGTTCCCG GCCTCAGCCG TTCAGCAAGG TTTCCAGGTG
    CAGGTCGGCT GTCACTCAGA TGATCCGAGC GCTAAAGAAA CGCTTAATCG AGCGCCGGCC
    GTTGTTCGCA GGATTCATGT TGATCGCCAG AAGGTTCCAG TGTCCAGTTT CTGGGGAGGC
    CTCCTGTACA TCATTGTGAG GTCTAACAGC AACTTTGGTA CCGTCCCAGT CAGGGTGTAT
    GGAGCAGAAC CAGCCCCAAT CTATATAAAG GGCAAAACCA GCCTGGATTC CTGGTTACAT
    TCAATCCGCC AGTTGCCTGC CCCTTGGGCC GAGCTGGTTG CAGAGAACAT TATCCTGACT
    GTCCCATCAG ATGCCGTTCG CTCACTAAAG AATCCAGAAG CCCTGCTGTC TCTTTGGGAC
    AGGATCATGG CGGCTATTGC TGACCTTGCA GCCAAACCCA AGAAATTTCC ACGACCTGAG
    CGTATTGTTG CAGATGTGCA GATCTCAGCT GGTTGGATGC ATGCTGGATA CCCCATCATG
    TGTGACTTGG AATCGGCTAA AGAGCTGACG GATCTACACC ATATGGAGAA AGAGGGGATC
    TGGGGGCCAA TTCATGAACT TGGCCACAAC CAACAGAAAA CACAGTGGGA GTTCCCACCC
    CATACCACGG AAGCCACGTG TAACCTCTGG GCTGTGTATG TGCATGAAAC GGTCCTTGGG
    ATCCCGCGGG ATAGAGCCCA CGGCAACCTT CAGCCCAACG TCCGGACGTC TCGTATCAAA
    AGTTACTTGC AGAATGGAGC CAACTTAGAG CAGTGGGATG GATGGACAGC GTTGGAGACC
    TACCTACAGC TGCAGGAAGG CTTGGGCTGG GACCCATTCA GGCGCCTATA CAGAGACCCG
    AGCATCTCCG GCATGAGCAA TGAAAACACA TTCAAGATGA ATCTGTGGGC GGAGAAGTTC
    TCTCGGGCCG CGCAGACCAA TCTGGTTCCG TTCTTTGAGG CCTGGGGATG GCCCATTGAC
    CAATTGACTC GCAGTAAACT CTCCGCATTG CCCGTATGGG AGAAGGATCC CATGAAGCCC
    TATGTTACTG CCAAGAAGTC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53015
    Clone ID Related Accession (Same CDS sequence) XM_031897381.1
    Accession Version XM_031897381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGCTTTT TAAAAAATGC TGTATCTTGG CTGAGGCCAA ACCCAGATGC CATTATTGGG 
    GTTCATACTA CATTACCAGT GTTAGCCCAT AATCTATCTG GGTACAAAGT CCGAAATACT
    TCCACCCTGA TTCAGGGCCT GGGCGTGTTC TGCACTAACG GATATGAGGC CCACCAAGCT
    GAGGAGATCA TCTCATTTGT GCGAGGGGGA GGCGGCCTGC TGATTGGGGC GCAGGCTTGG
    CACTGGTCCT ACAGCCATAA AGAGAATGTT CTGCACCATT TCCCAGGGAA CAAAATCATA
    TCTGTTTGTG GGGTGCACTT CACCAGTGAG TACGGAGAGA AAGGAGATTT CTGGGTGACT
    GAAGAGATGC CGCAGGCTCC AGTGTGCACA GAGAATATGG CGGCTAATGA AGATTACCAG
    TCTCTGGTCC GTGGCGTTGC GTCTCTGAGT TTCACTGGGG ATGCGGTTCC GTGCAAACTG
    CTGCTGACAG GACCCTGTGC ATTCCCGGTG CTGGTGTCGC CCCGAAAAGA TGTGCTCATC
    GCTGCCTCTC GCTATGGGAA AGGGAAAGTA GTGGTTCTGG CCCATGAAGG TTATCTTAGT
    ACACAGGAGT TTATGGGCTT TTTAAATAAT GCTGTATCTT GGCTGAGGCC AAACCCAGAT
    GCCGTTATTG GGGTTCATAC TACATTACCA GTGTTAGCCC ATAATCTATC TGGGTACAAA
    GTCCGAAATA CTTCCACCCT GATTCAGGGC CTGGGCGTGT TCTGCACTAA CGGATATGAG
    GCCCACCAAG CTGAGGAGAT CATCTCATTT GTGCGAGGGG GAGGCGGCCT GCTGATTGGG
    GCGCAGGCTT GGCACTGGTC CTACAGCCAT AAAGAGAATG TTCTGCACCA TTTCCCAGGG
    AACAAAATCA TATCTGTTTG TGGGGTGCAC TTCACCAGTG AGTACGGAGA GAAAGGAGAT
    TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT GCAGTGTGCA CAGATTACCA GTCTCTGGTC
    CGTGGCGTTG CGTCTCTGAG TTTCACTGGG GATCCGGTTC CGTGCAAACT GCTGCTGAGA
    AAACCAACTG CATTCCCGGT GCTGGTGTCG CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT
    CACTATGGGA AAGGGAAAGT AGTGGTTCTG GCCCATGAGG GTTATCTCAA CACAGAGGAG
    TTTATGGCTT TTCTGAAAAA TGCTGTATCT TGGCTGAGTC CTAATTCAGA TGCCGTTATT
    GGGGTTCATA CTACATTACC AGTGTTAGCC AATAATCTAT CTGGTGCCGG GTACAAAGTC
    CGAAATACTT CCACCCTGAT TCAGGGCCTG GGCGTGTTCT GCACTAACGG ATATGAGGCC
    CACCAAGCTG AGGAGATCAT CTCATTTGTG CGAGGGGGAG GCGGCCTGCT GATTGGGGCG
    CAGGCTTGGC ACTGGTCCTA CAGCCATAAA GAGAATGTTC TGCACCATTT CCCAGGGAAC
    AAAATCATAT CTGTTTGTGG GGTGCACTTC ACCAGTGAGT ACGGAGAGAA AGGAGATTTC
    TGGGTGACTG ATGAGATGCC GCAGGCTCCA GTGTGCACAG AGAATATGGC GGCTGATGAA
    GATTACCAGT CTCTGGTCCG TGGCGTTGCG TCTCTGAGTT TCACTGGGGA TGCGGTTCCG
    TGCAAACTGC TGCTGACAGG ACCCTGTGCA TTCCCGGTGC TGGTGTCGCC CCGAAAAGAT
    GTGCTCATCG CTGCCTCTCG CTATGGGAAA GGGAAAGTAG TGGTTCTGGC CCATGAGGGT
    TATCTTAGTA CACAGGAGTT TATGGGCTTT TTAAAAAATG CTGTATCTTG GCTGAGGCCA
    AACCCAGATG CCGTTATTGG GGTTCATACT ACATTAACAT TGTTAGCCCA TAATCTATTT
    GGGTACAAAA TCCGAAATAC TTCCACCCTG ATTCAGGGCC TGGGCGTGTT CTGCACTAAC
    GGATATGAGG CCCACCAAGC TGAGGAGATC ATCTCATTTG TGCGAGGGGG AGGCGGCCTG
    CTGATTGGGG CGCAGGCTTG GCACTGGTCC TACAGCCATA AAGAGAATGT TCTGCACCAT
    TTCCCAGGGA ACAAAATCAT ATCTGTTTGT GGGGTGCACT TCACCAGTGA GTACGGAGAG
    AAAGGAGATT TCTGGGTGAC TGATGAGATG CCGCAGGCTC CAGTGTGCAC AGAGAATATG
    GCGGCTGATG AAGATTACCA GTCTCTGGTC CGTGGTGTTG CGTCTCTGAG TTTCACTGGG
    GATGCGGTTC CGTGCAAACT GCTGCTGACA GGACCCTGTG CATTCCCGGT GCTGGTGTCG
    CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT CGCTATGGGA AAGGGAAAGT AGTGGTTCTG
    GCCCATGAGG GTTATCTTAG TACACAGGAG TTTATGGGCT TTTTAAAAAA TGCTGTATCT
    TGGCTGAGGC CAAACCCAGA TGTCATTATT GGGGTTCATA CTACATTACC AGTGTTAGCC
    CATAATCTAT CTGGGTACAA AGTCCGAAAT ACTTCCACCC TGATTCAGGG CCTGGGCGTG
    TTCTGCACTA ACGGATATGA GGCCCACCAA GCTGAGGAGA TCATCTCATT TGTGCGAGGG
    GGAGGCGGCC TGCTGATTGG GGCGCAGGCT TGGCACTGGT CCTACAGCCA TAAAGAGAAT
    GTTCTGCACC ATTTCCCAGG GAACAAAATC ATATCTGTTT GTGGGGTGCA CTTCACCAGT
    GAGTACGGAG AGAAAGGAGA TTTCTTGGTG ACTGAAGAGA TGCCGCAGGC TCCAGTGTGC
    ACAGATTATG ATTTCTCAGT GGGTCAGAAG TTCCTCCTGA ATGGCGTCTC TCAGCTGGAC
    ATCAGTGGGC CGAGTGTCCC CTCAGACCCC CTCTTGCACG GCGCTTCGTC CTTCCCAGTT
    GGGTCGAGTG AAGACCAACA GTGCTTTGTT GGAGCTGCAT ATTATGGCCA AGGGCGAGTG
    GTTGTAGCCA CACATGAAGA TTTCCTCTCC AAACCAGAAC TGAAGACTTT AATGCTTAAT
    GCCATATCTT GGCTGGACGT AGGGCAGAGA AGAATCGGAG TCCATGCCGA CCTGAGGGGC
    TTTGCTGAAG TTCTGCAGAA GGAAAACATC CCCTGTCGGG TATCAAGTTT AACCCCCAAG
    CTGAGTGTCT ACTGCTGCCC GTCCTACAGC GACGCTGAAG CAAAGGCCGT CCATCAGTTT
    GTTGCAGAGG GGGGAGGGCT TCTTATCGCT GGCCATGCCT GGTGGTGTTC CTATCAGAAT
    TCAGCCTGCA ATGTCCTCAA CTACCCTGGA AACAAAATAC TGAACAGGTT TGGGATTAGC
    ATTCTGTGTA GAGCCGTCCC TCAGGGCATC TATTGGGCAG TCAGTCCCAA TGAAGCCAGT
    CACCAAAATA ACTGCCACAG AGCACTTCAT CATTTGCAGG TTGCACTAAA GAGCGGTACC
    AAACTGCTAC AGCCCATCAG CACCAGGATG CGCAAACTCA GGCAGAATGT CAGCGCTTTT
    ATGAGGCACT CGGCCTGTTG TGTTCCCAAT GTCAGCAAGA CTTGTCCTGT GAGCGGCTGC
    TCCGAAGAAG CTTTACTGCT GAGTTTGGCC CACAAATCCC ATAATATGAG CCACAAGTGC
    GATGAATTGA GTATTCTGGA GAAGGAAACA GTCACTGTTA ATATTGATGC CACCAACCCA
    GGTGCCGACG CTTGGAGAAG CACCGGCCTT TATTTGCCCC CCAGAAAGGC AGTGGTTATT
    GAGTTCCCGG CCTCAGCCGT TCAGCAAGGT TTCCAGGTGC AGGTCGGCTG TCACTCAGAT
    GATCCGAGCG CTAAAGAAAC GCTTAATCGA GCGCCGGCCG TTGTTCGCAG GATTCATGTT
    GATCGCCAGA AGGTTCCAGT GTCCAGTTTC TGGGGAGGCC TCCTGTACAT CATTGTGAGG
    TCTAACAGCA ACTTTGGTAC CGTCCCAGTC AGGGTGTATG GAGCAGAACC AGCCCCAATC
    TATATAAAGG GCAAAACCAG CCTGGATTCC TGGTTACATT CAATCCGCCA GTTGCCTGCC
    CCTTGGGCCG AGCTGGTTGC AGAGAACATT ATCCTGACTG TCCCATCAGA TGCCGTTCGC
    TCACTAAAGA ATCCAGAAGC CCTGCTGTCT CTTTGGGACA GGATCATGGC GGCTATTGCT
    GACCTTGCAG CCAAACCCAA GAAATTTCCA CGACCTGAGC GTATTGTTGC AGATGTGCAG
    ATCTCAGCTG GTTGGATGCA TGCTGGATAC CCCATCATGT GTGACTTGGA ATCGGCTAAA
    GAGCTGACGG ATCTACACCA TATGGAGAAA GAGGGGATCT GGGGGCCAAT TCATGAACTT
    GGCCACAACC AACAGAAAAC ACAGTGGGAG TTCCCACCCC ATACCACGGA AGCCACGTGT
    AACCTCTGGG CTGTGTATGT GCATGAAACG GTCCTTGGGA TCCCGCGGGA TAGAGCCCAC
    GGCAACCTTC AGCCCAACGT CCGGACGTCT CGTATCAAAA GTTACTTGCA GAATGGAGCC
    AACTTAGAGC AGTGGGATGG ATGGACAGCG TTGGAGACCT ACCTACAGCT GCAGGAAGGC
    TTGGGCTGGG ACCCATTCAG GCGCCTATAC AGAGACCCGA GCATCTCCGG CATGAGCAAT
    GAAAACACAT TCAAGATGAA TCTGTGGGCG GAGAAGTTCT CTCGGGCCGC GCAGACCAAT
    CTGGTTCCGT TCTTTGAGGC CTGGGGATGG CCCATTGACC AATTGACTCG CAGTAAACTC
    TCCGCATTGC CCGTATGGGA GAAGGATCCC ATGAAGCCCT ATGTTACTGC CAAGAAGTCT
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753241.1
    CDS359..5224
    Translation

    Target ORF information:

    RefSeq Version XM_031897381.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGCTTTT TAAAAAATGC TGTATCTTGG CTGAGGCCAA ACCCAGATGC CATTATTGGG 
    GTTCATACTA CATTACCAGT GTTAGCCCAT AATCTATCTG GGTACAAAGT CCGAAATACT
    TCCACCCTGA TTCAGGGCCT GGGCGTGTTC TGCACTAACG GATATGAGGC CCACCAAGCT
    GAGGAGATCA TCTCATTTGT GCGAGGGGGA GGCGGCCTGC TGATTGGGGC GCAGGCTTGG
    CACTGGTCCT ACAGCCATAA AGAGAATGTT CTGCACCATT TCCCAGGGAA CAAAATCATA
    TCTGTTTGTG GGGTGCACTT CACCAGTGAG TACGGAGAGA AAGGAGATTT CTGGGTGACT
    GAAGAGATGC CGCAGGCTCC AGTGTGCACA GAGAATATGG CGGCTAATGA AGATTACCAG
    TCTCTGGTCC GTGGCGTTGC GTCTCTGAGT TTCACTGGGG ATGCGGTTCC GTGCAAACTG
    CTGCTGACAG GACCCTGTGC ATTCCCGGTG CTGGTGTCGC CCCGAAAAGA TGTGCTCATC
    GCTGCCTCTC GCTATGGGAA AGGGAAAGTA GTGGTTCTGG CCCATGAAGG TTATCTTAGT
    ACACAGGAGT TTATGGGCTT TTTAAATAAT GCTGTATCTT GGCTGAGGCC AAACCCAGAT
    GCCGTTATTG GGGTTCATAC TACATTACCA GTGTTAGCCC ATAATCTATC TGGGTACAAA
    GTCCGAAATA CTTCCACCCT GATTCAGGGC CTGGGCGTGT TCTGCACTAA CGGATATGAG
    GCCCACCAAG CTGAGGAGAT CATCTCATTT GTGCGAGGGG GAGGCGGCCT GCTGATTGGG
    GCGCAGGCTT GGCACTGGTC CTACAGCCAT AAAGAGAATG TTCTGCACCA TTTCCCAGGG
    AACAAAATCA TATCTGTTTG TGGGGTGCAC TTCACCAGTG AGTACGGAGA GAAAGGAGAT
    TTCTGGGTGA CTGAAGAGAT GCCGCAGGTT GCAGTGTGCA CAGATTACCA GTCTCTGGTC
    CGTGGCGTTG CGTCTCTGAG TTTCACTGGG GATCCGGTTC CGTGCAAACT GCTGCTGAGA
    AAACCAACTG CATTCCCGGT GCTGGTGTCG CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT
    CACTATGGGA AAGGGAAAGT AGTGGTTCTG GCCCATGAGG GTTATCTCAA CACAGAGGAG
    TTTATGGCTT TTCTGAAAAA TGCTGTATCT TGGCTGAGTC CTAATTCAGA TGCCGTTATT
    GGGGTTCATA CTACATTACC AGTGTTAGCC AATAATCTAT CTGGTGCCGG GTACAAAGTC
    CGAAATACTT CCACCCTGAT TCAGGGCCTG GGCGTGTTCT GCACTAACGG ATATGAGGCC
    CACCAAGCTG AGGAGATCAT CTCATTTGTG CGAGGGGGAG GCGGCCTGCT GATTGGGGCG
    CAGGCTTGGC ACTGGTCCTA CAGCCATAAA GAGAATGTTC TGCACCATTT CCCAGGGAAC
    AAAATCATAT CTGTTTGTGG GGTGCACTTC ACCAGTGAGT ACGGAGAGAA AGGAGATTTC
    TGGGTGACTG ATGAGATGCC GCAGGCTCCA GTGTGCACAG AGAATATGGC GGCTGATGAA
    GATTACCAGT CTCTGGTCCG TGGCGTTGCG TCTCTGAGTT TCACTGGGGA TGCGGTTCCG
    TGCAAACTGC TGCTGACAGG ACCCTGTGCA TTCCCGGTGC TGGTGTCGCC CCGAAAAGAT
    GTGCTCATCG CTGCCTCTCG CTATGGGAAA GGGAAAGTAG TGGTTCTGGC CCATGAGGGT
    TATCTTAGTA CACAGGAGTT TATGGGCTTT TTAAAAAATG CTGTATCTTG GCTGAGGCCA
    AACCCAGATG CCGTTATTGG GGTTCATACT ACATTAACAT TGTTAGCCCA TAATCTATTT
    GGGTACAAAA TCCGAAATAC TTCCACCCTG ATTCAGGGCC TGGGCGTGTT CTGCACTAAC
    GGATATGAGG CCCACCAAGC TGAGGAGATC ATCTCATTTG TGCGAGGGGG AGGCGGCCTG
    CTGATTGGGG CGCAGGCTTG GCACTGGTCC TACAGCCATA AAGAGAATGT TCTGCACCAT
    TTCCCAGGGA ACAAAATCAT ATCTGTTTGT GGGGTGCACT TCACCAGTGA GTACGGAGAG
    AAAGGAGATT TCTGGGTGAC TGATGAGATG CCGCAGGCTC CAGTGTGCAC AGAGAATATG
    GCGGCTGATG AAGATTACCA GTCTCTGGTC CGTGGTGTTG CGTCTCTGAG TTTCACTGGG
    GATGCGGTTC CGTGCAAACT GCTGCTGACA GGACCCTGTG CATTCCCGGT GCTGGTGTCG
    CCCCGAAAAG ATGTGCTCAT CGCTGCCTCT CGCTATGGGA AAGGGAAAGT AGTGGTTCTG
    GCCCATGAGG GTTATCTTAG TACACAGGAG TTTATGGGCT TTTTAAAAAA TGCTGTATCT
    TGGCTGAGGC CAAACCCAGA TGTCATTATT GGGGTTCATA CTACATTACC AGTGTTAGCC
    CATAATCTAT CTGGGTACAA AGTCCGAAAT ACTTCCACCC TGATTCAGGG CCTGGGCGTG
    TTCTGCACTA ACGGATATGA GGCCCACCAA GCTGAGGAGA TCATCTCATT TGTGCGAGGG
    GGAGGCGGCC TGCTGATTGG GGCGCAGGCT TGGCACTGGT CCTACAGCCA TAAAGAGAAT
    GTTCTGCACC ATTTCCCAGG GAACAAAATC ATATCTGTTT GTGGGGTGCA CTTCACCAGT
    GAGTACGGAG AGAAAGGAGA TTTCTTGGTG ACTGAAGAGA TGCCGCAGGC TCCAGTGTGC
    ACAGATTATG ATTTCTCAGT GGGTCAGAAG TTCCTCCTGA ATGGCGTCTC TCAGCTGGAC
    ATCAGTGGGC CGAGTGTCCC CTCAGACCCC CTCTTGCACG GCGCTTCGTC CTTCCCAGTT
    GGGTCGAGTG AAGACCAACA GTGCTTTGTT GGAGCTGCAT ATTATGGCCA AGGGCGAGTG
    GTTGTAGCCA CACATGAAGA TTTCCTCTCC AAACCAGAAC TGAAGACTTT AATGCTTAAT
    GCCATATCTT GGCTGGACGT AGGGCAGAGA AGAATCGGAG TCCATGCCGA CCTGAGGGGC
    TTTGCTGAAG TTCTGCAGAA GGAAAACATC CCCTGTCGGG TATCAAGTTT AACCCCCAAG
    CTGAGTGTCT ACTGCTGCCC GTCCTACAGC GACGCTGAAG CAAAGGCCGT CCATCAGTTT
    GTTGCAGAGG GGGGAGGGCT TCTTATCGCT GGCCATGCCT GGTGGTGTTC CTATCAGAAT
    TCAGCCTGCA ATGTCCTCAA CTACCCTGGA AACAAAATAC TGAACAGGTT TGGGATTAGC
    ATTCTGTGTA GAGCCGTCCC TCAGGGCATC TATTGGGCAG TCAGTCCCAA TGAAGCCAGT
    CACCAAAATA ACTGCCACAG AGCACTTCAT CATTTGCAGG TTGCACTAAA GAGCGGTACC
    AAACTGCTAC AGCCCATCAG CACCAGGATG CGCAAACTCA GGCAGAATGT CAGCGCTTTT
    ATGAGGCACT CGGCCTGTTG TGTTCCCAAT GTCAGCAAGA CTTGTCCTGT GAGCGGCTGC
    TCCGAAGAAG CTTTACTGCT GAGTTTGGCC CACAAATCCC ATAATATGAG CCACAAGTGC
    GATGAATTGA GTATTCTGGA GAAGGAAACA GTCACTGTTA ATATTGATGC CACCAACCCA
    GGTGCCGACG CTTGGAGAAG CACCGGCCTT TATTTGCCCC CCAGAAAGGC AGTGGTTATT
    GAGTTCCCGG CCTCAGCCGT TCAGCAAGGT TTCCAGGTGC AGGTCGGCTG TCACTCAGAT
    GATCCGAGCG CTAAAGAAAC GCTTAATCGA GCGCCGGCCG TTGTTCGCAG GATTCATGTT
    GATCGCCAGA AGGTTCCAGT GTCCAGTTTC TGGGGAGGCC TCCTGTACAT CATTGTGAGG
    TCTAACAGCA ACTTTGGTAC CGTCCCAGTC AGGGTGTATG GAGCAGAACC AGCCCCAATC
    TATATAAAGG GCAAAACCAG CCTGGATTCC TGGTTACATT CAATCCGCCA GTTGCCTGCC
    CCTTGGGCCG AGCTGGTTGC AGAGAACATT ATCCTGACTG TCCCATCAGA TGCCGTTCGC
    TCACTAAAGA ATCCAGAAGC CCTGCTGTCT CTTTGGGACA GGATCATGGC GGCTATTGCT
    GACCTTGCAG CCAAACCCAA GAAATTTCCA CGACCTGAGC GTATTGTTGC AGATGTGCAG
    ATCTCAGCTG GTTGGATGCA TGCTGGATAC CCCATCATGT GTGACTTGGA ATCGGCTAAA
    GAGCTGACGG ATCTACACCA TATGGAGAAA GAGGGGATCT GGGGGCCAAT TCATGAACTT
    GGCCACAACC AACAGAAAAC ACAGTGGGAG TTCCCACCCC ATACCACGGA AGCCACGTGT
    AACCTCTGGG CTGTGTATGT GCATGAAACG GTCCTTGGGA TCCCGCGGGA TAGAGCCCAC
    GGCAACCTTC AGCCCAACGT CCGGACGTCT CGTATCAAAA GTTACTTGCA GAATGGAGCC
    AACTTAGAGC AGTGGGATGG ATGGACAGCG TTGGAGACCT ACCTACAGCT GCAGGAAGGC
    TTGGGCTGGG ACCCATTCAG GCGCCTATAC AGAGACCCGA GCATCTCCGG CATGAGCAAT
    GAAAACACAT TCAAGATGAA TCTGTGGGCG GAGAAGTTCT CTCGGGCCGC GCAGACCAAT
    CTGGTTCCGT TCTTTGAGGC CTGGGGATGG CCCATTGACC AATTGACTCG CAGTAAACTC
    TCCGCATTGC CCGTATGGGA GAAGGATCCC ATGAAGCCCT ATGTTACTGC CAAGAAGTCT
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41059
    Clone ID Related Accession (Same CDS sequence) XM_018089944.1
    Accession Version XM_018089944.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683507.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTTTC TGAAAAATGC TGTATCTTGG CTGAGTCCTA ATTCAGATGC CGTTATTGGG 
    GTTCATACTA CATTACCAGT GTTAGCCAAT AATCTATCTG GTGCCGGGTA CAAAGTCCGA
    AATACTTCCA CCCTGATTCA GGGCCTGGGC GTGTTCTGCA CTAACGGATA TGAGGCCCAC
    CAAGCTGAGG AGATCATCTC ATTTGTGCGA GGGGGAGGCG GCCTGCTGAT TGGGGCGCAG
    GCTTGGCACT GGTCCTACAG CCATAAAGAG AATGTTCTGC ACCATTTCCC AGGGAACAAA
    ATCATATCTG TTTGTGGGGT GCACTTCACC AGTGAGTACG GAGAGAAAGG AGATTTCTGG
    GTGACTGATG AGATGCCGCA GGCTCCAGTG TGCACAGAGA ATATGGCGGC TGATGAAGAT
    TACCAGTCTC TGGTCCGTGG CGTTGCGTCT CTGAGTTTCA CTGGGGATGC GGTTCCGTGC
    AAACTGCTGC TGACAGGACC CTGTGCATTC CCGGTGCTGG TGTCACCCCG AAAAGATGTG
    CTCATCGCTG CCTCTCGCTA TGGGAAAGGG AAAGTAGTGG TTCTGGCCCA TGAGGGTTAT
    CTTAGTACAC AGGAGTTTAT GGGCTTTTTA AAAAATGCTG TATCTTGGCT GAGGCCAAAC
    CCAGATGCCG TTATTGGGAT TCATACTACA TTACCAGTGT TAGCCCATAA TCTATCTGGG
    TACAAAATCC AAAATACTTC CACCCTGATT CAGGGCCTGG GCGTGTTCTG CACTAACGGA
    TATGAGGCCC ACCAAGCTGA GGAGATCATC TCATTTGTGC GAGGGGGAGG CGGCCTGCTG
    ATTGGGGCGC AGGCTTGGCA CTGGTCCTAC AGCCATAAAG AGAATGTTCT GCACCATTTC
    CCAGGGAACA AAATCATATC TGTTTGTGGG GTGCACTTCA CCAGTGAGTA CGGAGAGAAA
    GGAGATTTCT GGGTGACTGA TGAGATGCCG CAGGTTGCAG TGTGCACAGA TTACCAGTCT
    CTGGTCCGTG GCGTTGCGTC TCTGAGTTTC ACTGGGGATC CGGTTCCGTG CAAACTGCTG
    CTGAGAAAAC CAACTGCATT CCCGGTGCTG GTGTCGCCCC GAAAAGATGT GCTCATCGCT
    GCCTCTCACT ATGGGAAAGG GAAAGTAGTG GTTCTGGCCC ATGAGGGTTA TCTCAACACA
    GAGGAGTTTA TGGCTTTTCT GAAAAATGCT GTATCTTGGC TGAGTCCTAA TTCAGATGCC
    GTTATTGGGG TTCATACTAC ATTACCAGTG TTAGCCAATA ATCTATCTGG TGCCGGGTAC
    AAAGTCCGAA ATACTTCCAC CCTGATTCAG GGCCTGGGCG TGTTCTGCAC TAACGGATAT
    GAGGCCCACC AAGCTGAGGA GATCATCTCA TTTGTGCGAG GGGGAGGCGG CCTGCTGATT
    GGGGCGCAGG CTTGGCACTG GTCCTACAGC CATAAAGAGA ATGTTCTGCA CCATTTCCCA
    GGGAACAAAA TCATATCTGT TTGTGGGGTG CACTTCACCA GTGAGTACGG AGAGAAAGGA
    GATTTCTGGG TGACTGATGA GATGCCGCAG GTTGCAGTGT GCACAGAGAA TATGGCGGCT
    GATGAAGATT ACCAGTCTCT GGTCCGTGGC GTTGCGTCTC TGAGTTTCAC TGGGGATGCG
    GTTCCGTGCA AACTGCTGCT GACAGGACCC TGTGCATTCC CGGTGCTGGT GTCACCCCGA
    AAAGATGTGC TCATCGCTGC CTCTCGCTAT GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT
    GAGGGCTATC TTAGTACACA GGAGTTTATG GGCTTTTTAA AAAATGCTGT ATCTTGGCTG
    AGGCCAAACC CAGATGCCGT TATTGGGGTT CATACTACAT TAACATTGTT AGCCCATAAT
    CTGTCTGGGT ACAAAATCCG AAATACTTCC ACCCTGATTC AGGGCCTGGG CGTGTTCTGC
    ACTAACGGAT ATGAGGCCCA CCAAGCTGAG GAGATCATCT CATTTGTGCG AGGGGGAGGC
    GGCCTGCTGA TTGGGGCGCA GGCTTGGCAC TGGTCCTACA GCCATAAAGA GAATGTTCTG
    CACCATTTCC CAGGGAACAA AATCATATCT GTTTGTGGGG TGCACTTCAC CAGTGAGTAC
    GGAGAGAAAG GAGATTTCTG GGTGACTGAT GAGATGCCGC AGGTTGCAGT GTGCACAGAT
    TACCAGTCTC TGGTCCGTGG CGTTGCGTCT CTGAGTTTCA CTGGGGATCC GGTTCCGTGC
    AAACTGCTGC TGAGAAAACC AACTGCATTC CCGGTGCTGG TGTCGCCCCG AAAAGATGTG
    CTCATCGCTG CCTCTCACTA TGGGAAAGGG AAAGTAGTGG TTCTGGCCCA TGAGGGTTAT
    CTCAACACAG AGGAGTTTAT GGCTTTTCTG AAAAATGCTG TATCTTGGCT GAGTCCTAAT
    TCAGATGCCG TTATTGGGGT TCATACTACA TTACCAGTGT TAGCCAATAA TCTATCTGGT
    GCCGGGTACA AAGTCCGAAA TACTTCCACC CTGATTCAGG GCCTGGGCGT GTTCTGCACT
    AACGGATATG AGGCCCACCA AGCTGAGGAG ATCATCTCAT TTGTGCGAGG GGGAGGCGGC
    CTGCTGATTG GGGCGCAGGC TTGGCACTGG TCCTACAGCC ATAAAGAGAA TGTTCTGCAC
    CATTTCCCAG GGAACAAAAT CATATCTGTT TGTGGGGTGC ACTTCACCAG TGAGTACGGA
    GAGAAAGGAG ATTTCTGGGT GACTGATGAG ATGCCGCAGG CTCCAGTGTG CACAGATTAT
    GACTTCTCAG TGGGTCAGAA GTTCCTCCTG AATGGCGTCT CTCAGCTGGA CATCAGTGGG
    CCGAGTGTCC CCTCAGACCC CCTCTTGCAC GGCGCTTCAT CCTTCCCAGT TGGGTCGAGT
    GAAGACCAAC AGTGCTTTGT TGGAGCTGCA TATTATGGCC AAGGGCGAGT GGTTGTAGCC
    ACACATGAAG ATTTCCTCTC CAAACCAGAA CTGAAGACTT TAATGCTTAA TGCCATATCT
    TGGCTGGACG TAGGGCAGAG AAGAATCGGA GTCCATGCCG ACCTGAGGGG CTTTGCTGAA
    GTTCTGCAGA AGGAAAACAT CCCCTGTCGG GTATCAAGTT TAACCCCCAA GCTGAGTGTC
    TACTGCTGCC CGTCCTACAG CGACGCTGAA GCAAAGGCCG TCCATCAGTT TGTTGCAGAG
    GGGGGAGGGC TTCTTATCGC TGGCCATGCC TGGTGGTGTT CCTATCAGAA TTCAGCCTGC
    AATGTCCTCA ACTACCCTGG AAACAAAATA CTGAACAGGT TTGGGATTAG CATTCTGTGT
    AGAGCCGTCC CTCAGGGCAT CTATTGGGCA GTCAGTCCCA ATGAAGCCAG TCACCAAAAT
    AACTGCCACA GAGCACTTCA TCATTTGCAG GTTGCACTAA AGAGCGGTAC CAAACTGCTA
    CAGCCCATCA GCACCAGGAT GCGCAAACTC AGGCAGAATG TCAGCGCTTT TATGAGGCAC
    TCGGCCTGTT GTGTTCCCAA AGTCAGCAAG ACTTGTCCTG TGAGCGGCTG CTCCGAAGAA
    GCTTTACTGC TGAGTTTGGC CCACAAATCC CATAATATGA GCCACAAGTG TGATGATTTG
    AGTATTCTGG AGAAGGAACC TTCAGTCACT GTTAATATTG ATGCCACCAA CCCAGGTGCC
    GACGCTTGGA GAAGCACCGG CCTTTATTTG CCCCCCAGAA AGGCAGTGGT TATTGAGTTC
    CCGGCCTCGG CCGTGCAGCA AGGGTTCCAG GTGCAGGTCG GCTGTCACTC AGATGATCCG
    AGCGCTAAAG AAACGCTTAA TCGAGCGCCG GTCGTTGTTC GCAGGATTCA TGTTGATCGC
    CAGAAGGTTC CAGTGTCCAG TTTCTGGGGA GGCCTCCTGT ACATCATTGT GAGGTCTAAC
    AGCAACTTTG GTACCGTCCC AGTCAGGGTG TATGGAGCAG AACCAGCCCC AATCTATATA
    AAGGGCAAAA CCAGCCTGGA TTCCTGGTTA CATTCAATCC GCCAGTTGCC TGCCCCTTGG
    GCCGAGCTGG TTACAGAGAA CATTATCCTG ACTGTCCCAT CAGATGCCGT TCGCTCACTA
    AAGAATCCAG AAGCCCTGCT GTCTCTTTGG GACAGGATCA TGGCGGCTAT TGCTGACCTT
    GCAGCCAAAC CCAAGAAATT TCCACGACCT GAGCGTATTG TTGCAGATGT GCAGATCTCA
    GCTGGTTGGA TGCATGCTGG ATACCCCATC ATGTGTGACT TGGAATCGGC TAAAGAGCTG
    ACGGATCTAC ACCATATGGA GAAAGAGGGG ATCTGGGGGC CAATTCATGA ACTTGGCCAC
    AACCAACAGA AAACACAGTG GGAGTTCCCA CCCCATACCA CGGAAGCCAC GTGTAACCTC
    TGGGCTGTGT ATGTGCATGA AACGGTCCTT GGGATCCCGC GGGATAGAGC CCACGGCAAC
    CTTCAGCCCA ACGTCCGGAC GTCTCGTATC AAAAGTTACT TGCAGAATGG AGCCAACTTA
    GAGCAGTGGG ATGGATGGAC AGCGTTGGAG ACCTACCTAC AGCTGCAGGA AGGCTTGGGC
    TGGGACCCAT TCAGGCGCCT ATACAGAGAC CCGAGCATCT CCGGCATGAG CAATGAAAAC
    ACATTCAAGA TGAATCTGTG GGCGGAGAAG TTCTCTCGGG CCGCGCAGAC CAATCTGGTT
    CCGTTCTTTG AGGCCTGGGG ATGGCCCATT GACCAATCGA CTCGCAGTAA ACTCTCCGCA
    TTGCCCGTAT GGGAGAAGGA TCCCATGAAG CCCTATGTTA CTGCCAAGAA GTCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945433.1
    CDS407..5266
    Translation

    Target ORF information:

    RefSeq Version XM_018089944.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor homolog (LOC100489234), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089944.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTTTC TGAAAAATGC TGTATCTTGG CTGAGTCCTA ATTCAGATGC CGTTATTGGG 
    GTTCATACTA CATTACCAGT GTTAGCCAAT AATCTATCTG GTGCCGGGTA CAAAGTCCGA
    AATACTTCCA CCCTGATTCA GGGCCTGGGC GTGTTCTGCA CTAACGGATA TGAGGCCCAC
    CAAGCTGAGG AGATCATCTC ATTTGTGCGA GGGGGAGGCG GCCTGCTGAT TGGGGCGCAG
    GCTTGGCACT GGTCCTACAG CCATAAAGAG AATGTTCTGC ACCATTTCCC AGGGAACAAA
    ATCATATCTG TTTGTGGGGT GCACTTCACC AGTGAGTACG GAGAGAAAGG AGATTTCTGG
    GTGACTGATG AGATGCCGCA GGCTCCAGTG TGCACAGAGA ATATGGCGGC TGATGAAGAT
    TACCAGTCTC TGGTCCGTGG CGTTGCGTCT CTGAGTTTCA CTGGGGATGC GGTTCCGTGC
    AAACTGCTGC TGACAGGACC CTGTGCATTC CCGGTGCTGG TGTCACCCCG AAAAGATGTG
    CTCATCGCTG CCTCTCGCTA TGGGAAAGGG AAAGTAGTGG TTCTGGCCCA TGAGGGTTAT
    CTTAGTACAC AGGAGTTTAT GGGCTTTTTA AAAAATGCTG TATCTTGGCT GAGGCCAAAC
    CCAGATGCCG TTATTGGGAT TCATACTACA TTACCAGTGT TAGCCCATAA TCTATCTGGG
    TACAAAATCC AAAATACTTC CACCCTGATT CAGGGCCTGG GCGTGTTCTG CACTAACGGA
    TATGAGGCCC ACCAAGCTGA GGAGATCATC TCATTTGTGC GAGGGGGAGG CGGCCTGCTG
    ATTGGGGCGC AGGCTTGGCA CTGGTCCTAC AGCCATAAAG AGAATGTTCT GCACCATTTC
    CCAGGGAACA AAATCATATC TGTTTGTGGG GTGCACTTCA CCAGTGAGTA CGGAGAGAAA
    GGAGATTTCT GGGTGACTGA TGAGATGCCG CAGGTTGCAG TGTGCACAGA TTACCAGTCT
    CTGGTCCGTG GCGTTGCGTC TCTGAGTTTC ACTGGGGATC CGGTTCCGTG CAAACTGCTG
    CTGAGAAAAC CAACTGCATT CCCGGTGCTG GTGTCGCCCC GAAAAGATGT GCTCATCGCT
    GCCTCTCACT ATGGGAAAGG GAAAGTAGTG GTTCTGGCCC ATGAGGGTTA TCTCAACACA
    GAGGAGTTTA TGGCTTTTCT GAAAAATGCT GTATCTTGGC TGAGTCCTAA TTCAGATGCC
    GTTATTGGGG TTCATACTAC ATTACCAGTG TTAGCCAATA ATCTATCTGG TGCCGGGTAC
    AAAGTCCGAA ATACTTCCAC CCTGATTCAG GGCCTGGGCG TGTTCTGCAC TAACGGATAT
    GAGGCCCACC AAGCTGAGGA GATCATCTCA TTTGTGCGAG GGGGAGGCGG CCTGCTGATT
    GGGGCGCAGG CTTGGCACTG GTCCTACAGC CATAAAGAGA ATGTTCTGCA CCATTTCCCA
    GGGAACAAAA TCATATCTGT TTGTGGGGTG CACTTCACCA GTGAGTACGG AGAGAAAGGA
    GATTTCTGGG TGACTGATGA GATGCCGCAG GTTGCAGTGT GCACAGAGAA TATGGCGGCT
    GATGAAGATT ACCAGTCTCT GGTCCGTGGC GTTGCGTCTC TGAGTTTCAC TGGGGATGCG
    GTTCCGTGCA AACTGCTGCT GACAGGACCC TGTGCATTCC CGGTGCTGGT GTCACCCCGA
    AAAGATGTGC TCATCGCTGC CTCTCGCTAT GGGAAAGGGA AAGTAGTGGT TCTGGCCCAT
    GAGGGCTATC TTAGTACACA GGAGTTTATG GGCTTTTTAA AAAATGCTGT ATCTTGGCTG
    AGGCCAAACC CAGATGCCGT TATTGGGGTT CATACTACAT TAACATTGTT AGCCCATAAT
    CTGTCTGGGT ACAAAATCCG AAATACTTCC ACCCTGATTC AGGGCCTGGG CGTGTTCTGC
    ACTAACGGAT ATGAGGCCCA CCAAGCTGAG GAGATCATCT CATTTGTGCG AGGGGGAGGC
    GGCCTGCTGA TTGGGGCGCA GGCTTGGCAC TGGTCCTACA GCCATAAAGA GAATGTTCTG
    CACCATTTCC CAGGGAACAA AATCATATCT GTTTGTGGGG TGCACTTCAC CAGTGAGTAC
    GGAGAGAAAG GAGATTTCTG GGTGACTGAT GAGATGCCGC AGGTTGCAGT GTGCACAGAT
    TACCAGTCTC TGGTCCGTGG CGTTGCGTCT CTGAGTTTCA CTGGGGATCC GGTTCCGTGC
    AAACTGCTGC TGAGAAAACC AACTGCATTC CCGGTGCTGG TGTCGCCCCG AAAAGATGTG
    CTCATCGCTG CCTCTCACTA TGGGAAAGGG AAAGTAGTGG TTCTGGCCCA TGAGGGTTAT
    CTCAACACAG AGGAGTTTAT GGCTTTTCTG AAAAATGCTG TATCTTGGCT GAGTCCTAAT
    TCAGATGCCG TTATTGGGGT TCATACTACA TTACCAGTGT TAGCCAATAA TCTATCTGGT
    GCCGGGTACA AAGTCCGAAA TACTTCCACC CTGATTCAGG GCCTGGGCGT GTTCTGCACT
    AACGGATATG AGGCCCACCA AGCTGAGGAG ATCATCTCAT TTGTGCGAGG GGGAGGCGGC
    CTGCTGATTG GGGCGCAGGC TTGGCACTGG TCCTACAGCC ATAAAGAGAA TGTTCTGCAC
    CATTTCCCAG GGAACAAAAT CATATCTGTT TGTGGGGTGC ACTTCACCAG TGAGTACGGA
    GAGAAAGGAG ATTTCTGGGT GACTGATGAG ATGCCGCAGG CTCCAGTGTG CACAGATTAT
    GACTTCTCAG TGGGTCAGAA GTTCCTCCTG AATGGCGTCT CTCAGCTGGA CATCAGTGGG
    CCGAGTGTCC CCTCAGACCC CCTCTTGCAC GGCGCTTCAT CCTTCCCAGT TGGGTCGAGT
    GAAGACCAAC AGTGCTTTGT TGGAGCTGCA TATTATGGCC AAGGGCGAGT GGTTGTAGCC
    ACACATGAAG ATTTCCTCTC CAAACCAGAA CTGAAGACTT TAATGCTTAA TGCCATATCT
    TGGCTGGACG TAGGGCAGAG AAGAATCGGA GTCCATGCCG ACCTGAGGGG CTTTGCTGAA
    GTTCTGCAGA AGGAAAACAT CCCCTGTCGG GTATCAAGTT TAACCCCCAA GCTGAGTGTC
    TACTGCTGCC CGTCCTACAG CGACGCTGAA GCAAAGGCCG TCCATCAGTT TGTTGCAGAG
    GGGGGAGGGC TTCTTATCGC TGGCCATGCC TGGTGGTGTT CCTATCAGAA TTCAGCCTGC
    AATGTCCTCA ACTACCCTGG AAACAAAATA CTGAACAGGT TTGGGATTAG CATTCTGTGT
    AGAGCCGTCC CTCAGGGCAT CTATTGGGCA GTCAGTCCCA ATGAAGCCAG TCACCAAAAT
    AACTGCCACA GAGCACTTCA TCATTTGCAG GTTGCACTAA AGAGCGGTAC CAAACTGCTA
    CAGCCCATCA GCACCAGGAT GCGCAAACTC AGGCAGAATG TCAGCGCTTT TATGAGGCAC
    TCGGCCTGTT GTGTTCCCAA AGTCAGCAAG ACTTGTCCTG TGAGCGGCTG CTCCGAAGAA
    GCTTTACTGC TGAGTTTGGC CCACAAATCC CATAATATGA GCCACAAGTG TGATGATTTG
    AGTATTCTGG AGAAGGAACC TTCAGTCACT GTTAATATTG ATGCCACCAA CCCAGGTGCC
    GACGCTTGGA GAAGCACCGG CCTTTATTTG CCCCCCAGAA AGGCAGTGGT TATTGAGTTC
    CCGGCCTCGG CCGTGCAGCA AGGGTTCCAG GTGCAGGTCG GCTGTCACTC AGATGATCCG
    AGCGCTAAAG AAACGCTTAA TCGAGCGCCG GTCGTTGTTC GCAGGATTCA TGTTGATCGC
    CAGAAGGTTC CAGTGTCCAG TTTCTGGGGA GGCCTCCTGT ACATCATTGT GAGGTCTAAC
    AGCAACTTTG GTACCGTCCC AGTCAGGGTG TATGGAGCAG AACCAGCCCC AATCTATATA
    AAGGGCAAAA CCAGCCTGGA TTCCTGGTTA CATTCAATCC GCCAGTTGCC TGCCCCTTGG
    GCCGAGCTGG TTACAGAGAA CATTATCCTG ACTGTCCCAT CAGATGCCGT TCGCTCACTA
    AAGAATCCAG AAGCCCTGCT GTCTCTTTGG GACAGGATCA TGGCGGCTAT TGCTGACCTT
    GCAGCCAAAC CCAAGAAATT TCCACGACCT GAGCGTATTG TTGCAGATGT GCAGATCTCA
    GCTGGTTGGA TGCATGCTGG ATACCCCATC ATGTGTGACT TGGAATCGGC TAAAGAGCTG
    ACGGATCTAC ACCATATGGA GAAAGAGGGG ATCTGGGGGC CAATTCATGA ACTTGGCCAC
    AACCAACAGA AAACACAGTG GGAGTTCCCA CCCCATACCA CGGAAGCCAC GTGTAACCTC
    TGGGCTGTGT ATGTGCATGA AACGGTCCTT GGGATCCCGC GGGATAGAGC CCACGGCAAC
    CTTCAGCCCA ACGTCCGGAC GTCTCGTATC AAAAGTTACT TGCAGAATGG AGCCAACTTA
    GAGCAGTGGG ATGGATGGAC AGCGTTGGAG ACCTACCTAC AGCTGCAGGA AGGCTTGGGC
    TGGGACCCAT TCAGGCGCCT ATACAGAGAC CCGAGCATCT CCGGCATGAG CAATGAAAAC
    ACATTCAAGA TGAATCTGTG GGCGGAGAAG TTCTCTCGGG CCGCGCAGAC CAATCTGGTT
    CCGTTCTTTG AGGCCTGGGG ATGGCCCATT GACCAATCGA CTCGCAGTAA ACTCTCCGCA
    TTGCCCGTAT GGGAGAAGGA TCCCATGAAG CCCTATGTTA CTGCCAAGAA GTCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.