tcaf2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following tcaf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tcaf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa12933 XM_002934581.5
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor 2 (tcaf2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa43328 XM_018092375.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor 2 (tcaf2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa54971 XM_031899106.1
Latest version!
Xenopus tropicalis TRPM8 channel-associated factor 2 (tcaf2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OXa12933 XM_002934581.4 Xenopus tropicalis TRPM8 channel-associated factor 2 (tcaf2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa12933
    Clone ID Related Accession (Same CDS sequence) XM_002934581.4 , XM_002934581.5
    Accession Version XM_002934581.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934581.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAAGTTC ATGAAGATTA CCACTCTTTG GTCCATTGTA TAGGTTCTCT GGATTTCTCT 
    GGGGAAGCTG TTCCATGCAA GCTGTTGCTA ACAGGAGACA CTGCATTCCC TGTGCTGGTA
    ACTCCAAAAA AAGATGTGCT CATTGCTGCT GCTCGTTATG GAAAAGGAAA AGTAGTGGTT
    ATGGCCCATG AGGGTTATCT AAACATGAAT GAGTTTATGG ACTTTTTGAA AAATGCGGTA
    TCTTGGTTAA GGCCAAATTC AGAAGCCAAC ATTGGGGTGC AAAATGCATT ACATCTGCTT
    GCAGATAATC TCTCTGTCTA CGGTTCCAGA GTCCAAAAGA CTTCCACCCT GACTGAGGGC
    CTAGGAGTGT TCTGTACTAA TGGATATGAT GATAGCCAGG CTCAGCAGAT CATTTCATTT
    GTGCGAGAAG GGGGAGGCCT GCTGATTGGC GCTCAGGCTT GGCACTGGTC CTACAGCCAC
    AGAGAGGAAA ATGTCCTCCA CCATTTTCCA GGAAACAAAA TTATATCAGT TTCTGGGGTG
    TACTTTACCA GTGACTATGG AGAGAAAGGA AAATTCTGTG TGACTGAGAA TATACCACAG
    ACTACAATTT ATACAAGTTT TGACTTCTCA CTCGACCAGA AGTACCTTCT GAATGGCATG
    TCTCAGCTGG ACATCAGTGG GTCAAATATT CCTTCAGATC TTCTATTGCA TGGTGCTTTG
    TCATTTCCAA TTGGGCTAAG TGAAAACAAG CAATGTTTTC TTGGTGCTAC ATATTATGGC
    AAAGGACGAG TGGTTGTAGC AACACATGAA AGTTACCTAT CCAAACCAGA GCTCAAAACA
    TTAATGCTCA AAGTCATATC TTGGCTAGAT ATAAACCAGA ACAGAAGAAT TGGTGTTCAC
    AAGAACCTGG GAAGCTTTGC TGAGCTTTTG AAGGGAGAAA ACATCCCCTG TAATGTATCT
    AGTTTAGTCT CTGATCTAAG TGTTTACTGC TGCAAGTCCT ACAGTGATGC AGAAGCAAAG
    GCCATCCATC AGTTTGTTGC AGAGGGTGGA GGACTTCTTA TTGCTGGCCA TGCCTGGTAT
    TGGTCCTATC AGAATTCAGA CTTGGATGTC CTTACCCACT ACCCTGGAAA CAAGATATTG
    AATAAGTTTG GCATTAGCAT ACTGGATAGA ACCATTCCTG AGGGCAATTA TAAGGCAATC
    AATCCAGATA AAACCAATGA ACAGTATCAC TTTCACAGAG CACTTTGTAA TTTGCAAACT
    GAACTTCAGA GCAGTGCAGA ACTGAAACCT CCCTTGAGCA CCTGGATGAT CAAGCTTAGG
    CAGGATGTCA CCGCTTTTAT GAGGCTCCCA GCCAGTCCTA TCATCAGCTC CATTCAGAGT
    CAGTTTGTAG AAATGATGCG AACATGTGAT ATACCTAAGG TCAGCAAGGC GTGTCCTGTG
    AGCAGCTGCT CCAAAGAAGC ATTAATCTTG TGTTTGGCCC ATGAAACCTT TACTGTGAGC
    CAGGAGTGTG ATGAATCAAA TAATCTGGAA AAGGGACCTT CAGTCACTGT TGAGATTGAT
    GGCACAAACC CTGGTAATGA TGCCTGGAGA AGCACTGGCC TGTACTTGGC TCCCAGAAAG
    ACAGCAGTTC TTGAGTTTCC TGCGTCAGTT GTGCAGCAAG GTCTTAAGGT GCAGGTTGGC
    TGTCAGGCAG ACGATTTGAG CTCTGCAGAA AAGTATTGTC GTGCCCCTGT TGTTGTTCGC
    AAATGTCATG TTGATAGACA AAAAGTTTCA GTGTCCTGTT TCTGGGGAGG ACTTCTATAC
    ATCATTGTGA AAGCTAACAG CAATTTGGGT ATAATCCCAG TTAAGGTGTA TGGAGCAGAA
    CCAGCCCCAG TCTATATAAA AGGTAAGACT AACCTTGACT CATGGTTACA ATCAGTTCGC
    TATTTGCCTG CTCCATGGGC AGAGCTGATT ACAGAGAACA TTATCTTAAC CGTACCCTCA
    GATGCTATTC GCTCACTAAG TGATCCAGAA GCGCTGCTGT CTCTTTGGGA CAAGATAATG
    GTCTCTATTA CTGAGCTTGC AGCCATACCA AAGACATTTC CACGTCCTGA GCGATTTGTT
    GCAGATGTGC AGATCTCAGC AGGTTGGATG CATGCTGGAT ATCCCATTAT GTGCCACTTG
    GAATCAGCTA AAGAACTGAC TGACCTAAAC GTTATGCAGA CAGTAGGAAT CTGGGGTCCA
    ATACATGAAC TAGGACATAA TCAGCAGAAA ATAAACTGGG AGTTTCCACC CCACACAACG
    GAAGCCACAT GTAATCTATG GTCTGTGTAT GTGCATGAAA CAGTTCTGGG AATCCCACGG
    AATAAAGCAC ACGGTTCTCT ACAGCCAGAA ACCAGGGCCT GTTATATACA GGAATATGTG
    AAAAATGGAT CCAATTTGGA ACAATGGGAT GTATGGACAG CATTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCGGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGCA
    ACTCGCATTA AATTATCTGC ATTGCCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATCA AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934627.2
    CDS112..2829
    Translation

    Target ORF information:

    RefSeq Version XM_002934581.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor 2 (tcaf2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934581.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAAGTTC ATGAAGATTA CCACTCTTTG GTCCATTGTA TAGGTTCTCT GGATTTCTCT 
    GGGGAAGCTG TTCCATGCAA GCTGTTGCTA ACAGGAGACA CTGCATTCCC TGTGCTGGTA
    ACTCCAAAAA AAGATGTGCT CATTGCTGCT GCTCGTTATG GAAAAGGAAA AGTAGTGGTT
    ATGGCCCATG AGGGTTATCT AAACATGAAT GAGTTTATGG ACTTTTTGAA AAATGCGGTA
    TCTTGGTTAA GGCCAAATTC AGAAGCCAAC ATTGGGGTGC AAAATGCATT ACATCTGCTT
    GCAGATAATC TCTCTGTCTA CGGTTCCAGA GTCCAAAAGA CTTCCACCCT GACTGAGGGC
    CTAGGAGTGT TCTGTACTAA TGGATATGAT GATAGCCAGG CTCAGCAGAT CATTTCATTT
    GTGCGAGAAG GGGGAGGCCT GCTGATTGGC GCTCAGGCTT GGCACTGGTC CTACAGCCAC
    AGAGAGGAAA ATGTCCTCCA CCATTTTCCA GGAAACAAAA TTATATCAGT TTCTGGGGTG
    TACTTTACCA GTGACTATGG AGAGAAAGGA AAATTCTGTG TGACTGAGAA TATACCACAG
    ACTACAATTT ATACAAGTTT TGACTTCTCA CTCGACCAGA AGTACCTTCT GAATGGCATG
    TCTCAGCTGG ACATCAGTGG GTCAAATATT CCTTCAGATC TTCTATTGCA TGGTGCTTTG
    TCATTTCCAA TTGGGCTAAG TGAAAACAAG CAATGTTTTC TTGGTGCTAC ATATTATGGC
    AAAGGACGAG TGGTTGTAGC AACACATGAA AGTTACCTAT CCAAACCAGA GCTCAAAACA
    TTAATGCTCA AAGTCATATC TTGGCTAGAT ATAAACCAGA ACAGAAGAAT TGGTGTTCAC
    AAGAACCTGG GAAGCTTTGC TGAGCTTTTG AAGGGAGAAA ACATCCCCTG TAATGTATCT
    AGTTTAGTCT CTGATCTAAG TGTTTACTGC TGCAAGTCCT ACAGTGATGC AGAAGCAAAG
    GCCATCCATC AGTTTGTTGC AGAGGGTGGA GGACTTCTTA TTGCTGGCCA TGCCTGGTAT
    TGGTCCTATC AGAATTCAGA CTTGGATGTC CTTACCCACT ACCCTGGAAA CAAGATATTG
    AATAAGTTTG GCATTAGCAT ACTGGATAGA ACCATTCCTG AGGGCAATTA TAAGGCAATC
    AATCCAGATA AAACCAATGA ACAGTATCAC TTTCACAGAG CACTTTGTAA TTTGCAAACT
    GAACTTCAGA GCAGTGCAGA ACTGAAACCT CCCTTGAGCA CCTGGATGAT CAAGCTTAGG
    CAGGATGTCA CCGCTTTTAT GAGGCTCCCA GCCAGTCCTA TCATCAGCTC CATTCAGAGT
    CAGTTTGTAG AAATGATGCG AACATGTGAT ATACCTAAGG TCAGCAAGGC GTGTCCTGTG
    AGCAGCTGCT CCAAAGAAGC ATTAATCTTG TGTTTGGCCC ATGAAACCTT TACTGTGAGC
    CAGGAGTGTG ATGAATCAAA TAATCTGGAA AAGGGACCTT CAGTCACTGT TGAGATTGAT
    GGCACAAACC CTGGTAATGA TGCCTGGAGA AGCACTGGCC TGTACTTGGC TCCCAGAAAG
    ACAGCAGTTC TTGAGTTTCC TGCGTCAGTT GTGCAGCAAG GTCTTAAGGT GCAGGTTGGC
    TGTCAGGCAG ACGATTTGAG CTCTGCAGAA AAGTATTGTC GTGCCCCTGT TGTTGTTCGC
    AAATGTCATG TTGATAGACA AAAAGTTTCA GTGTCCTGTT TCTGGGGAGG ACTTCTATAC
    ATCATTGTGA AAGCTAACAG CAATTTGGGT ATAATCCCAG TTAAGGTGTA TGGAGCAGAA
    CCAGCCCCAG TCTATATAAA AGGTAAGACT AACCTTGACT CATGGTTACA ATCAGTTCGC
    TATTTGCCTG CTCCATGGGC AGAGCTGATT ACAGAGAACA TTATCTTAAC CGTACCCTCA
    GATGCTATTC GCTCACTAAG TGATCCAGAA GCGCTGCTGT CTCTTTGGGA CAAGATAATG
    GTCTCTATTA CTGAGCTTGC AGCCATACCA AAGACATTTC CACGTCCTGA GCGATTTGTT
    GCAGATGTGC AGATCTCAGC AGGTTGGATG CATGCTGGAT ATCCCATTAT GTGCCACTTG
    GAATCAGCTA AAGAACTGAC TGACCTAAAC GTTATGCAGA CAGTAGGAAT CTGGGGTCCA
    ATACATGAAC TAGGACATAA TCAGCAGAAA ATAAACTGGG AGTTTCCACC CCACACAACG
    GAAGCCACAT GTAATCTATG GTCTGTGTAT GTGCATGAAA CAGTTCTGGG AATCCCACGG
    AATAAAGCAC ACGGTTCTCT ACAGCCAGAA ACCAGGGCCT GTTATATACA GGAATATGTG
    AAAAATGGAT CCAATTTGGA ACAATGGGAT GTATGGACAG CATTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCGGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGCA
    ACTCGCATTA AATTATCTGC ATTGCCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATCA AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12933
    Clone ID Related Accession (Same CDS sequence) XM_002934581.4 , XM_002934581.5
    Accession Version XM_002934581.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934581.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAAGTTC ATGAAGATTA CCACTCTTTG GTCCATTGTA TAGGTTCTCT GGATTTCTCT 
    GGGGAAGCTG TTCCATGCAA GCTGTTGCTA ACAGGAGACA CTGCATTCCC TGTGCTGGTA
    ACTCCAAAAA AAGATGTGCT CATTGCTGCT GCTCGTTATG GAAAAGGAAA AGTAGTGGTT
    ATGGCCCATG AGGGTTATCT AAACATGAAT GAGTTTATGG ACTTTTTGAA AAATGCGGTA
    TCTTGGTTAA GGCCAAATTC AGAAGCCAAC ATTGGGGTGC AAAATGCATT ACATCTGCTT
    GCAGATAATC TCTCTGTCTA CGGTTCCAGA GTCCAAAAGA CTTCCACCCT GACTGAGGGC
    CTAGGAGTGT TCTGTACTAA TGGATATGAT GATAGCCAGG CTCAGCAGAT CATTTCATTT
    GTGCGAGAAG GGGGAGGCCT GCTGATTGGC GCTCAGGCTT GGCACTGGTC CTACAGCCAC
    AGAGAGGAAA ATGTCCTCCA CCATTTTCCA GGAAACAAAA TTATATCAGT TTCTGGGGTG
    TACTTTACCA GTGACTATGG AGAGAAAGGA AAATTCTGTG TGACTGAGAA TATACCACAG
    ACTACAATTT ATACAAGTTT TGACTTCTCA CTCGACCAGA AGTACCTTCT GAATGGCATG
    TCTCAGCTGG ACATCAGTGG GTCAAATATT CCTTCAGATC TTCTATTGCA TGGTGCTTTG
    TCATTTCCAA TTGGGCTAAG TGAAAACAAG CAATGTTTTC TTGGTGCTAC ATATTATGGC
    AAAGGACGAG TGGTTGTAGC AACACATGAA AGTTACCTAT CCAAACCAGA GCTCAAAACA
    TTAATGCTCA AAGTCATATC TTGGCTAGAT ATAAACCAGA ACAGAAGAAT TGGTGTTCAC
    AAGAACCTGG GAAGCTTTGC TGAGCTTTTG AAGGGAGAAA ACATCCCCTG TAATGTATCT
    AGTTTAGTCT CTGATCTAAG TGTTTACTGC TGCAAGTCCT ACAGTGATGC AGAAGCAAAG
    GCCATCCATC AGTTTGTTGC AGAGGGTGGA GGACTTCTTA TTGCTGGCCA TGCCTGGTAT
    TGGTCCTATC AGAATTCAGA CTTGGATGTC CTTACCCACT ACCCTGGAAA CAAGATATTG
    AATAAGTTTG GCATTAGCAT ACTGGATAGA ACCATTCCTG AGGGCAATTA TAAGGCAATC
    AATCCAGATA AAACCAATGA ACAGTATCAC TTTCACAGAG CACTTTGTAA TTTGCAAACT
    GAACTTCAGA GCAGTGCAGA ACTGAAACCT CCCTTGAGCA CCTGGATGAT CAAGCTTAGG
    CAGGATGTCA CCGCTTTTAT GAGGCTCCCA GCCAGTCCTA TCATCAGCTC CATTCAGAGT
    CAGTTTGTAG AAATGATGCG AACATGTGAT ATACCTAAGG TCAGCAAGGC GTGTCCTGTG
    AGCAGCTGCT CCAAAGAAGC ATTAATCTTG TGTTTGGCCC ATGAAACCTT TACTGTGAGC
    CAGGAGTGTG ATGAATCAAA TAATCTGGAA AAGGGACCTT CAGTCACTGT TGAGATTGAT
    GGCACAAACC CTGGTAATGA TGCCTGGAGA AGCACTGGCC TGTACTTGGC TCCCAGAAAG
    ACAGCAGTTC TTGAGTTTCC TGCGTCAGTT GTGCAGCAAG GTCTTAAGGT GCAGGTTGGC
    TGTCAGGCAG ACGATTTGAG CTCTGCAGAA AAGTATTGTC GTGCCCCTGT TGTTGTTCGC
    AAATGTCATG TTGATAGACA AAAAGTTTCA GTGTCCTGTT TCTGGGGAGG ACTTCTATAC
    ATCATTGTGA AAGCTAACAG CAATTTGGGT ATAATCCCAG TTAAGGTGTA TGGAGCAGAA
    CCAGCCCCAG TCTATATAAA AGGTAAGACT AACCTTGACT CATGGTTACA ATCAGTTCGC
    TATTTGCCTG CTCCATGGGC AGAGCTGATT ACAGAGAACA TTATCTTAAC CGTACCCTCA
    GATGCTATTC GCTCACTAAG TGATCCAGAA GCGCTGCTGT CTCTTTGGGA CAAGATAATG
    GTCTCTATTA CTGAGCTTGC AGCCATACCA AAGACATTTC CACGTCCTGA GCGATTTGTT
    GCAGATGTGC AGATCTCAGC AGGTTGGATG CATGCTGGAT ATCCCATTAT GTGCCACTTG
    GAATCAGCTA AAGAACTGAC TGACCTAAAC GTTATGCAGA CAGTAGGAAT CTGGGGTCCA
    ATACATGAAC TAGGACATAA TCAGCAGAAA ATAAACTGGG AGTTTCCACC CCACACAACG
    GAAGCCACAT GTAATCTATG GTCTGTGTAT GTGCATGAAA CAGTTCTGGG AATCCCACGG
    AATAAAGCAC ACGGTTCTCT ACAGCCAGAA ACCAGGGCCT GTTATATACA GGAATATGTG
    AAAAATGGAT CCAATTTGGA ACAATGGGAT GTATGGACAG CATTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCGGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGCA
    ACTCGCATTA AATTATCTGC ATTGCCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATCA AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934627.2
    CDS112..2829
    Translation

    Target ORF information:

    RefSeq Version XM_002934581.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor 2 (tcaf2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934581.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAAGTTC ATGAAGATTA CCACTCTTTG GTCCATTGTA TAGGTTCTCT GGATTTCTCT 
    GGGGAAGCTG TTCCATGCAA GCTGTTGCTA ACAGGAGACA CTGCATTCCC TGTGCTGGTA
    ACTCCAAAAA AAGATGTGCT CATTGCTGCT GCTCGTTATG GAAAAGGAAA AGTAGTGGTT
    ATGGCCCATG AGGGTTATCT AAACATGAAT GAGTTTATGG ACTTTTTGAA AAATGCGGTA
    TCTTGGTTAA GGCCAAATTC AGAAGCCAAC ATTGGGGTGC AAAATGCATT ACATCTGCTT
    GCAGATAATC TCTCTGTCTA CGGTTCCAGA GTCCAAAAGA CTTCCACCCT GACTGAGGGC
    CTAGGAGTGT TCTGTACTAA TGGATATGAT GATAGCCAGG CTCAGCAGAT CATTTCATTT
    GTGCGAGAAG GGGGAGGCCT GCTGATTGGC GCTCAGGCTT GGCACTGGTC CTACAGCCAC
    AGAGAGGAAA ATGTCCTCCA CCATTTTCCA GGAAACAAAA TTATATCAGT TTCTGGGGTG
    TACTTTACCA GTGACTATGG AGAGAAAGGA AAATTCTGTG TGACTGAGAA TATACCACAG
    ACTACAATTT ATACAAGTTT TGACTTCTCA CTCGACCAGA AGTACCTTCT GAATGGCATG
    TCTCAGCTGG ACATCAGTGG GTCAAATATT CCTTCAGATC TTCTATTGCA TGGTGCTTTG
    TCATTTCCAA TTGGGCTAAG TGAAAACAAG CAATGTTTTC TTGGTGCTAC ATATTATGGC
    AAAGGACGAG TGGTTGTAGC AACACATGAA AGTTACCTAT CCAAACCAGA GCTCAAAACA
    TTAATGCTCA AAGTCATATC TTGGCTAGAT ATAAACCAGA ACAGAAGAAT TGGTGTTCAC
    AAGAACCTGG GAAGCTTTGC TGAGCTTTTG AAGGGAGAAA ACATCCCCTG TAATGTATCT
    AGTTTAGTCT CTGATCTAAG TGTTTACTGC TGCAAGTCCT ACAGTGATGC AGAAGCAAAG
    GCCATCCATC AGTTTGTTGC AGAGGGTGGA GGACTTCTTA TTGCTGGCCA TGCCTGGTAT
    TGGTCCTATC AGAATTCAGA CTTGGATGTC CTTACCCACT ACCCTGGAAA CAAGATATTG
    AATAAGTTTG GCATTAGCAT ACTGGATAGA ACCATTCCTG AGGGCAATTA TAAGGCAATC
    AATCCAGATA AAACCAATGA ACAGTATCAC TTTCACAGAG CACTTTGTAA TTTGCAAACT
    GAACTTCAGA GCAGTGCAGA ACTGAAACCT CCCTTGAGCA CCTGGATGAT CAAGCTTAGG
    CAGGATGTCA CCGCTTTTAT GAGGCTCCCA GCCAGTCCTA TCATCAGCTC CATTCAGAGT
    CAGTTTGTAG AAATGATGCG AACATGTGAT ATACCTAAGG TCAGCAAGGC GTGTCCTGTG
    AGCAGCTGCT CCAAAGAAGC ATTAATCTTG TGTTTGGCCC ATGAAACCTT TACTGTGAGC
    CAGGAGTGTG ATGAATCAAA TAATCTGGAA AAGGGACCTT CAGTCACTGT TGAGATTGAT
    GGCACAAACC CTGGTAATGA TGCCTGGAGA AGCACTGGCC TGTACTTGGC TCCCAGAAAG
    ACAGCAGTTC TTGAGTTTCC TGCGTCAGTT GTGCAGCAAG GTCTTAAGGT GCAGGTTGGC
    TGTCAGGCAG ACGATTTGAG CTCTGCAGAA AAGTATTGTC GTGCCCCTGT TGTTGTTCGC
    AAATGTCATG TTGATAGACA AAAAGTTTCA GTGTCCTGTT TCTGGGGAGG ACTTCTATAC
    ATCATTGTGA AAGCTAACAG CAATTTGGGT ATAATCCCAG TTAAGGTGTA TGGAGCAGAA
    CCAGCCCCAG TCTATATAAA AGGTAAGACT AACCTTGACT CATGGTTACA ATCAGTTCGC
    TATTTGCCTG CTCCATGGGC AGAGCTGATT ACAGAGAACA TTATCTTAAC CGTACCCTCA
    GATGCTATTC GCTCACTAAG TGATCCAGAA GCGCTGCTGT CTCTTTGGGA CAAGATAATG
    GTCTCTATTA CTGAGCTTGC AGCCATACCA AAGACATTTC CACGTCCTGA GCGATTTGTT
    GCAGATGTGC AGATCTCAGC AGGTTGGATG CATGCTGGAT ATCCCATTAT GTGCCACTTG
    GAATCAGCTA AAGAACTGAC TGACCTAAAC GTTATGCAGA CAGTAGGAAT CTGGGGTCCA
    ATACATGAAC TAGGACATAA TCAGCAGAAA ATAAACTGGG AGTTTCCACC CCACACAACG
    GAAGCCACAT GTAATCTATG GTCTGTGTAT GTGCATGAAA CAGTTCTGGG AATCCCACGG
    AATAAAGCAC ACGGTTCTCT ACAGCCAGAA ACCAGGGCCT GTTATATACA GGAATATGTG
    AAAAATGGAT CCAATTTGGA ACAATGGGAT GTATGGACAG CATTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCGGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGCA
    ACTCGCATTA AATTATCTGC ATTGCCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATCA AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43328
    Clone ID Related Accession (Same CDS sequence) XM_018092375.1
    Accession Version XM_018092375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAAGTTC ATGAAGATTA CCACTCTTTG GTCCATTGTA TAGGTTCTCT GGATTTCTCT 
    GGGGAAGCTG TTCCATGCAA GCTGTTGCTA ACAGGAGACA CTGCATTCCC TGTGCTGGTA
    ACTCCAAAAA AAGATGTGCT CATTGCTGCT GCTCGTTATG GAAAAGGAAA AGTAGTGGTT
    ATGGCCCATG AGGGTTATCT AAACATGAAT GAGTTTATGG ACTTTTTGAA AAATGCGGTA
    TCTTGGTTAA GGCCAAATTC AGAAGCCAAC ATTGGGGTGC AAAATGCATT ACATCTGCTT
    GCAGATAATC TCTCTGTCTA CGGTTCCAGA GTCCAAAAGA CTTCCACCCT GACTGAGGGC
    CTAGGAGTGT TCTGTACTAA TGGATATGAT GATAGCCAGG CTCAGCAGAT CATTTCATTT
    GTGCGAGAAG GGGGAGGCCT GCTGATTGGC GCTCAGGCTT GGCACTGGTC CTACAGCCAC
    AGAGAGGAAA ATGTCCTCCA CCATTTTCCA GGAAACAAAA TTATATCAGT TTCTGGGGTG
    TACTTTACCA GTGACTATGG AGAGAAAGGA AAATTCTGTG TGACTGAGAA TATACCACAG
    ACTACAATTT ATACAAGTTT TGACTTCTCA CTCGACCAGA AGTACCTTCT GAATGGCATG
    TCTCAGCTGG ACATCAGTGG GTCAAATATT CCTTCAGATC TTCTATTGCA TGGTGCTTTG
    TCATTTCCAA TTGGGCTAAG TGAAAACAAG CAATGTTTTC TTGGTGCTAC ATATTATGGC
    AAAGGACGAG TGGTTGTAGC AACACATGAA AGTTACCTAT CCAAACCAGA GCTCAAAACA
    TTAATGCTCA AAGTCATATC TTGGCTAGAT ATAAACCAGA ACAGAAGAAT TGGTGTTCAC
    AAGAACCTGG GAAGCTTTGC TGAGCTTTTG AAGGGAGAAA ACATCCCCTG TAATGTATCT
    AGTTTAGTCT CTGATCTAAG TGTTTACTGC TGCAAGTCCT ACAGTGATGC AGAAGCAAAG
    GCCATCCATC AGTTTGTTGC AGAGGGTGGA GGACTTCTTA TTGCTGGCCA TGCCTGGTAT
    TGGTCCTATC AGAATTCAGA CTTGGATGTC CTTACCCACT ACCCTGGAAA CAAGATATTG
    AATAAGTTTG GCATTAGCAT ACTGGATAGA ACCATTCCTG AGGGCAATTA TAAGGCAATC
    AATCCAGATA AAACCAATGA ACAGTATCAC TTTCACAGAG CACTTTGTAA TTTGCAAACT
    GAACTTCAGA GCAGTGCAGA ACTGAAACCT CCCTTGAGCA CCTGGATGAT CAAGCTTAGG
    CAGGATGTCA CCGCTTTTAT GAGGCTCCCA GCCAGTCCTA TCATCAGCTC CATTCAGAGT
    CAGTTTGTAG AAATGATGCG AACATGTGAT ATACCTAAGG TCAGCAAGGC GTGTCCTGTG
    AGCAGCTGCT CCAAAGAAGC ATTAATCTTG TGTTTGGCCC ATGAAACCTT TACTGTGAGC
    CAGGAGTGTG ATGAATCAAA TAATCTGGAA AAGGGACCTT CAGTCACTGT TGAGATTGAT
    GGCACAAACC CTGGTAATGA TGCCTGGAGA AGCACTGGCC TGTACTTGGC TCCCAGAAAG
    ACAGCAGTTC TTGAGTTTCC TGCGTCAGTT GTGCAGCAAG GTCTTAAGGT GCAGGTTGGC
    TGTCAGGCAG ACGATTTGAG CTCTGCAGAA AAGTATTGTC GTGCCCCTGT TGTTGTTCGC
    AAATGTCATG TTGATAGACA AAAAGTTTCA GTGTCCTGTT TCTGGGGAGG ACTTCTATAC
    ATCATTGTGA AAGCTAACAG CAATTTGGGT ATAATCCCAG TTAAGGTGTA TGGAGCAGAA
    CCAGCCCCAG TCTATATAAA AGGTAAGACT AACCTTGACT CATGGTTACA ATCAGTTCGC
    TATTTGCCTG CTCCATGGGC AGAGCTGATT ACAGAGAACA TTATCTTAAC CGTACCCTCA
    GATGCTATTC GCTCACTAAG TGATCCAGAA GCGCTGCTGT CTCTTTGGGA CAAGATAATG
    GTCTCTATTA CTGAGCTTGC AGCCATACCA AAGACATTTC CACGTCCTGA GCGATTTGTT
    GCAGATGTGC AGATCTCAGC AGGTTGGATG CATGCTGGAT ATCCCATTAT GTGCCACTTG
    GAATCAGCTA AAGAACTGAC TGACCTAAAC GTTATGCAGA CAGTAGGAAT CTGGGGTCCA
    ATACATGAAC TAGGACATAA TCAGCAGAAA ATAAACTGGG AGTTTCCACC CCACACAACG
    GAAGCCACAT GTAATCTATG GTCTGTGTAT GTGCATGAAA CAGTTCTGGG AATCCCACGG
    AATAAAGCAC ACGGTTCTCT ACAGCCAGAA ACCAGGGCCT GTTATATACA GGAATATGTG
    AAAAATGGAT CCAATTTGGA ACAATGGGAT GTATGGACAG CATTGGAGAC CTATCTACAG
    TTGACCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947864.1
    CDS112..2583
    Translation

    Target ORF information:

    RefSeq Version XM_018092375.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor 2 (tcaf2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAAGTTC ATGAAGATTA CCACTCTTTG GTCCATTGTA TAGGTTCTCT GGATTTCTCT 
    GGGGAAGCTG TTCCATGCAA GCTGTTGCTA ACAGGAGACA CTGCATTCCC TGTGCTGGTA
    ACTCCAAAAA AAGATGTGCT CATTGCTGCT GCTCGTTATG GAAAAGGAAA AGTAGTGGTT
    ATGGCCCATG AGGGTTATCT AAACATGAAT GAGTTTATGG ACTTTTTGAA AAATGCGGTA
    TCTTGGTTAA GGCCAAATTC AGAAGCCAAC ATTGGGGTGC AAAATGCATT ACATCTGCTT
    GCAGATAATC TCTCTGTCTA CGGTTCCAGA GTCCAAAAGA CTTCCACCCT GACTGAGGGC
    CTAGGAGTGT TCTGTACTAA TGGATATGAT GATAGCCAGG CTCAGCAGAT CATTTCATTT
    GTGCGAGAAG GGGGAGGCCT GCTGATTGGC GCTCAGGCTT GGCACTGGTC CTACAGCCAC
    AGAGAGGAAA ATGTCCTCCA CCATTTTCCA GGAAACAAAA TTATATCAGT TTCTGGGGTG
    TACTTTACCA GTGACTATGG AGAGAAAGGA AAATTCTGTG TGACTGAGAA TATACCACAG
    ACTACAATTT ATACAAGTTT TGACTTCTCA CTCGACCAGA AGTACCTTCT GAATGGCATG
    TCTCAGCTGG ACATCAGTGG GTCAAATATT CCTTCAGATC TTCTATTGCA TGGTGCTTTG
    TCATTTCCAA TTGGGCTAAG TGAAAACAAG CAATGTTTTC TTGGTGCTAC ATATTATGGC
    AAAGGACGAG TGGTTGTAGC AACACATGAA AGTTACCTAT CCAAACCAGA GCTCAAAACA
    TTAATGCTCA AAGTCATATC TTGGCTAGAT ATAAACCAGA ACAGAAGAAT TGGTGTTCAC
    AAGAACCTGG GAAGCTTTGC TGAGCTTTTG AAGGGAGAAA ACATCCCCTG TAATGTATCT
    AGTTTAGTCT CTGATCTAAG TGTTTACTGC TGCAAGTCCT ACAGTGATGC AGAAGCAAAG
    GCCATCCATC AGTTTGTTGC AGAGGGTGGA GGACTTCTTA TTGCTGGCCA TGCCTGGTAT
    TGGTCCTATC AGAATTCAGA CTTGGATGTC CTTACCCACT ACCCTGGAAA CAAGATATTG
    AATAAGTTTG GCATTAGCAT ACTGGATAGA ACCATTCCTG AGGGCAATTA TAAGGCAATC
    AATCCAGATA AAACCAATGA ACAGTATCAC TTTCACAGAG CACTTTGTAA TTTGCAAACT
    GAACTTCAGA GCAGTGCAGA ACTGAAACCT CCCTTGAGCA CCTGGATGAT CAAGCTTAGG
    CAGGATGTCA CCGCTTTTAT GAGGCTCCCA GCCAGTCCTA TCATCAGCTC CATTCAGAGT
    CAGTTTGTAG AAATGATGCG AACATGTGAT ATACCTAAGG TCAGCAAGGC GTGTCCTGTG
    AGCAGCTGCT CCAAAGAAGC ATTAATCTTG TGTTTGGCCC ATGAAACCTT TACTGTGAGC
    CAGGAGTGTG ATGAATCAAA TAATCTGGAA AAGGGACCTT CAGTCACTGT TGAGATTGAT
    GGCACAAACC CTGGTAATGA TGCCTGGAGA AGCACTGGCC TGTACTTGGC TCCCAGAAAG
    ACAGCAGTTC TTGAGTTTCC TGCGTCAGTT GTGCAGCAAG GTCTTAAGGT GCAGGTTGGC
    TGTCAGGCAG ACGATTTGAG CTCTGCAGAA AAGTATTGTC GTGCCCCTGT TGTTGTTCGC
    AAATGTCATG TTGATAGACA AAAAGTTTCA GTGTCCTGTT TCTGGGGAGG ACTTCTATAC
    ATCATTGTGA AAGCTAACAG CAATTTGGGT ATAATCCCAG TTAAGGTGTA TGGAGCAGAA
    CCAGCCCCAG TCTATATAAA AGGTAAGACT AACCTTGACT CATGGTTACA ATCAGTTCGC
    TATTTGCCTG CTCCATGGGC AGAGCTGATT ACAGAGAACA TTATCTTAAC CGTACCCTCA
    GATGCTATTC GCTCACTAAG TGATCCAGAA GCGCTGCTGT CTCTTTGGGA CAAGATAATG
    GTCTCTATTA CTGAGCTTGC AGCCATACCA AAGACATTTC CACGTCCTGA GCGATTTGTT
    GCAGATGTGC AGATCTCAGC AGGTTGGATG CATGCTGGAT ATCCCATTAT GTGCCACTTG
    GAATCAGCTA AAGAACTGAC TGACCTAAAC GTTATGCAGA CAGTAGGAAT CTGGGGTCCA
    ATACATGAAC TAGGACATAA TCAGCAGAAA ATAAACTGGG AGTTTCCACC CCACACAACG
    GAAGCCACAT GTAATCTATG GTCTGTGTAT GTGCATGAAA CAGTTCTGGG AATCCCACGG
    AATAAAGCAC ACGGTTCTCT ACAGCCAGAA ACCAGGGCCT GTTATATACA GGAATATGTG
    AAAAATGGAT CCAATTTGGA ACAATGGGAT GTATGGACAG CATTGGAGAC CTATCTACAG
    TTGACCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54971
    Clone ID Related Accession (Same CDS sequence) XM_031899106.1
    Accession Version XM_031899106.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2478bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TRPM8 channel-associated factor homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAAGTTC ATGAAGATTA CCACTCTTTG GTCCATTGTA TAGGTTCTCT GGATTTCTCT 
    GGGGAAGCTG TTCCATGCAA GCTGTTGCTA ACAGGAGACA CTGCATTCCC TGTGCTGGTA
    ACTCCAAAAA AAGATGTGCT CATTGCTGCT GCTCGTTATG GAAAAGGAAA AGTAGTGGTT
    ATGGCCCATG AGGGTTATCT AAACATGAAT GAGTTTATGG ACTTTTTGAA AAATGCGGTA
    TCTTGGTTAA GGCCAAATTC AGAAGCCAAC ATTGGGGTGC AAAATGCATT ACATCTGCTT
    GCAGATAATC TCTCTGTCTA CGGTTCCAGA GTCCAAAAGA CTTCCACCCT GACTGAGGGC
    CTAGGAGTGT TCTGTACTAA TGGATATGAT GATAGCCAGG CTCAGCAGAT CATTTCATTT
    GTGCGAGAAG GGGGAGGCCT GCTGATTGGC GCTCAGGCTT GGCACTGGTC CTACAGCCAC
    AGAGAGGAAA ATGTCCTCCA CCATTTTCCA GGAAACAAAA TTATATCAGT TTCTGGGGTG
    TACTTTACCA GTGACTATGG AGAGAAAGGA AAATTCTGTG TGACTGAGAA TATACCACAG
    ACTACAATTT ATACAAGTTT TGACTTCTCA CTCGACCAGA AGTACCTTCT GAATGGCATG
    TCTCAGCTGG ACATCAGTGG GTCAAATATT CCTTCAGATC TTCTATTGCA TGGTGCTTTG
    TCATTTCCAA TTGGGCTAAG TGAAAACAAG CAATGTTTTC TTGGTGCTAC ATATTATGGC
    AAAGGACGAG TGGTTGTAGC AACACATGAA AGTTACCTAT CCAAACCAGA GCTCAAAACA
    TTAATGCTCA AAGTCATATC TTGGCTAGAT ATAAACCAGA ACAGAAGAAT TGGTGTTCAC
    AAGAACCTGG GAAGCTTTGC TGAGCTTTTG AAGGGAGAAA ACATCCCCTG TAATGTATCT
    AGTTTAGTCT CTGATCTAAG TGTTTACTGC TGCAAGTCCT ACAGTGATGC AGAAGCAAAG
    GCCATCCATC AGTTTGTTGC AGAGGGTGGA GGACTTCTTA TTGCTGGCCA TGCCTGGTAT
    TGGTCCTATC AGAATTCAGA CTTGGATGTC CTTACCCACT ACCCTGGAAA CAAGATATTG
    AATAAGTTTG GCATTAGCAT ACTGGATAGA ACCATTCCTG AGGGCAATTA TAAGGCAATC
    AATCCAGATA AAACCAATGA ACAGTATCAC TTTCACAGAG CACTTTGTAA TTTGCAAACT
    GAACTTCAGA GCAGTGCAGA ACTGAAACCT CCCTTGAGCA CCTGGATGAT CAAGCTTAGG
    CAGGATGTCA CCGCTTTTAT GAGGCTCCCA GCCAGTCCTA TCATCAGCTC CATTCAGAGT
    CAGTTTGTAG AAATGATGCG AACATGTGAT ATACCTAAGG TCAGCAAGGC GTGTCCTGTG
    AGCAGCTGCT CCAAAGAAGC ATTAATCTTG TGTTTGGCCC ATGAAACCTT TACTGTGAGC
    CAGGAGTGTG ATGAATCAAA TAATCTGGAA AAGGGACCTT CAGTCACTGT TGAGATTGAT
    GGCACAAACC CTGGTAATGA TGCCTGGAGA AGCACTGGCC TGTACTTGGC TCCCAGAAAG
    ACAGCAGTTC TTGAGTTTCC TGCGTCAGTT GTGCAGCAAG GTCTTAAGGT GCAGGTTGGC
    TGTCAGGCAG ACGATTTGAG CTCTGCAGAA AAGTATTGTC GTGCCCCTGT TGTTGTTCGC
    AAATGTCATG TTGATAGACA AAAAGTTTCA GTGTCCTGTT TCTGGGGAGG ACTTCTATAC
    ATCATTGTGA AAGCTAACAG CAATTTGGGT ATAATCCCAG TTAAGGTGTA TGGAGCAGAA
    CCAGCCCCAG TCTATATAAA AGGTTGGATG CATGCTGGAT ATCCCATTAT GTGCCACTTG
    GAATCAGCTA AAGAACTGAC TGACCTAAAC GTTATGCAGA CAGTAGGAAT CTGGGGTCCA
    ATACATGAAC TAGGACATAA TCAGCAGAAA ATAAACTGGG AGTTTCCACC CCACACAACG
    GAAGCCACAT GTAATCTATG GTCTGTGTAT GTGCATGAAA CAGTTCTGGG AATCCCACGG
    AATAAAGCAC ACGGTTCTCT ACAGCCAGAA ACCAGGGCCT GTTATATACA GGAATATGTG
    AAAAATGGAT CCAATTTGGA ACAATGGGAT GTATGGACAG CATTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCGGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGCA
    ACTCGCATTA AATTATCTGC ATTGCCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATCA AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754966.1
    CDS112..2589
    Translation

    Target ORF information:

    RefSeq Version XM_031899106.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TRPM8 channel-associated factor 2 (tcaf2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899106.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAAGTTC ATGAAGATTA CCACTCTTTG GTCCATTGTA TAGGTTCTCT GGATTTCTCT 
    GGGGAAGCTG TTCCATGCAA GCTGTTGCTA ACAGGAGACA CTGCATTCCC TGTGCTGGTA
    ACTCCAAAAA AAGATGTGCT CATTGCTGCT GCTCGTTATG GAAAAGGAAA AGTAGTGGTT
    ATGGCCCATG AGGGTTATCT AAACATGAAT GAGTTTATGG ACTTTTTGAA AAATGCGGTA
    TCTTGGTTAA GGCCAAATTC AGAAGCCAAC ATTGGGGTGC AAAATGCATT ACATCTGCTT
    GCAGATAATC TCTCTGTCTA CGGTTCCAGA GTCCAAAAGA CTTCCACCCT GACTGAGGGC
    CTAGGAGTGT TCTGTACTAA TGGATATGAT GATAGCCAGG CTCAGCAGAT CATTTCATTT
    GTGCGAGAAG GGGGAGGCCT GCTGATTGGC GCTCAGGCTT GGCACTGGTC CTACAGCCAC
    AGAGAGGAAA ATGTCCTCCA CCATTTTCCA GGAAACAAAA TTATATCAGT TTCTGGGGTG
    TACTTTACCA GTGACTATGG AGAGAAAGGA AAATTCTGTG TGACTGAGAA TATACCACAG
    ACTACAATTT ATACAAGTTT TGACTTCTCA CTCGACCAGA AGTACCTTCT GAATGGCATG
    TCTCAGCTGG ACATCAGTGG GTCAAATATT CCTTCAGATC TTCTATTGCA TGGTGCTTTG
    TCATTTCCAA TTGGGCTAAG TGAAAACAAG CAATGTTTTC TTGGTGCTAC ATATTATGGC
    AAAGGACGAG TGGTTGTAGC AACACATGAA AGTTACCTAT CCAAACCAGA GCTCAAAACA
    TTAATGCTCA AAGTCATATC TTGGCTAGAT ATAAACCAGA ACAGAAGAAT TGGTGTTCAC
    AAGAACCTGG GAAGCTTTGC TGAGCTTTTG AAGGGAGAAA ACATCCCCTG TAATGTATCT
    AGTTTAGTCT CTGATCTAAG TGTTTACTGC TGCAAGTCCT ACAGTGATGC AGAAGCAAAG
    GCCATCCATC AGTTTGTTGC AGAGGGTGGA GGACTTCTTA TTGCTGGCCA TGCCTGGTAT
    TGGTCCTATC AGAATTCAGA CTTGGATGTC CTTACCCACT ACCCTGGAAA CAAGATATTG
    AATAAGTTTG GCATTAGCAT ACTGGATAGA ACCATTCCTG AGGGCAATTA TAAGGCAATC
    AATCCAGATA AAACCAATGA ACAGTATCAC TTTCACAGAG CACTTTGTAA TTTGCAAACT
    GAACTTCAGA GCAGTGCAGA ACTGAAACCT CCCTTGAGCA CCTGGATGAT CAAGCTTAGG
    CAGGATGTCA CCGCTTTTAT GAGGCTCCCA GCCAGTCCTA TCATCAGCTC CATTCAGAGT
    CAGTTTGTAG AAATGATGCG AACATGTGAT ATACCTAAGG TCAGCAAGGC GTGTCCTGTG
    AGCAGCTGCT CCAAAGAAGC ATTAATCTTG TGTTTGGCCC ATGAAACCTT TACTGTGAGC
    CAGGAGTGTG ATGAATCAAA TAATCTGGAA AAGGGACCTT CAGTCACTGT TGAGATTGAT
    GGCACAAACC CTGGTAATGA TGCCTGGAGA AGCACTGGCC TGTACTTGGC TCCCAGAAAG
    ACAGCAGTTC TTGAGTTTCC TGCGTCAGTT GTGCAGCAAG GTCTTAAGGT GCAGGTTGGC
    TGTCAGGCAG ACGATTTGAG CTCTGCAGAA AAGTATTGTC GTGCCCCTGT TGTTGTTCGC
    AAATGTCATG TTGATAGACA AAAAGTTTCA GTGTCCTGTT TCTGGGGAGG ACTTCTATAC
    ATCATTGTGA AAGCTAACAG CAATTTGGGT ATAATCCCAG TTAAGGTGTA TGGAGCAGAA
    CCAGCCCCAG TCTATATAAA AGGTTGGATG CATGCTGGAT ATCCCATTAT GTGCCACTTG
    GAATCAGCTA AAGAACTGAC TGACCTAAAC GTTATGCAGA CAGTAGGAAT CTGGGGTCCA
    ATACATGAAC TAGGACATAA TCAGCAGAAA ATAAACTGGG AGTTTCCACC CCACACAACG
    GAAGCCACAT GTAATCTATG GTCTGTGTAT GTGCATGAAA CAGTTCTGGG AATCCCACGG
    AATAAAGCAC ACGGTTCTCT ACAGCCAGAA ACCAGGGCCT GTTATATACA GGAATATGTG
    AAAAATGGAT CCAATTTGGA ACAATGGGAT GTATGGACAG CATTGGAGAC CTATCTACAG
    TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCGGTTAT TCAAAGACTA CCAGAGCATG
    TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG
    GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGCA
    ACTCGCATTA AATTATCTGC ATTGCCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT
    AGTGCAATCA AATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.