Tcaf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tcaf2
Entrez Gene ID	232748
Full Name	TRPM8 channel-associated factor 2
Synonyms	Fam115c
General protein information	Preferred Names TRPM8 channel-associated factor 2 Names TRPM8 channel-associated factor 2 TRP channel-associated factor 2 family with sequence similarity 115, member C protein FAM115C
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 B2.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_146174.1	NP_666286.1	TRPM8 channel-associated factor 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	FAM115C	NP_775949.2
Canis lupus familiaris (dog)	FAM115C	XP_539847.2
Xenopus tropicalis (tropical clawed frog)	LOC100485132	XP_002941113.2
Xenopus tropicalis (tropical clawed frog)	LOC100498358	XP_002934625.2
Mus musculus (house mouse)	Fam115c	NP_666286.1
Danio rerio (zebrafish)	LOC100535998	XP_003200030.1
Xenopus tropicalis (tropical clawed frog)	LOC100489234	XP_002943401.2
Pan troglodytes (chimpanzee)	FAM115C	XP_519453.3
	FAM115C	XP_001092570.2
Bos taurus (cattle)	FAM115C	NP_001095394.1
Xenopus tropicalis (tropical clawed frog)	fam115c	XP_002934627.2
Danio rerio (zebrafish)	LOC100535908	XP_003200029.1
Danio rerio (zebrafish)	zgc:162193	NP_001082796.1
Danio rerio (zebrafish)	LOC555689	XP_683371.5
Xenopus tropicalis (tropical clawed frog)	fam115a	XP_002934606.1

Gene Ontology
Bibliography

Related articles in PubMed

TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.
Gkika D, Lemonnier L, Shapovalov G, Gordienko D, Poux C, Bernardini M, Bokhobza A, Bidaux G, Degerny C, Verreman K, Guarmit B, Benahmed M, de Launoit Y, Bindels RJ, Fiorio Pla A, Prevarskaya N
The Journal of cell biology208(1)89-107(2015 Jan)

Haploinsufficiency of SAMD9L, an endosome fusion facilitator, causes myeloid malignancies in mice mimicking human diseases with monosomy 7.
Nagamachi A, Matsui H, Asou H, Ozaki Y, Aki D, Kanai A, Takubo K, Suda T, Nakamura T, Wolff L, Honda H, Inaba T
Cancer cell24(3)305-17(2013 Sep)

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Kitamura H, Nakagawa T, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report novel TRP channel-associated factors that modulate TRPM8 activity.
Title: TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.

The following Tcaf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcaf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18029	NM_146174.1 Latest version!	Mus musculus TRPM8 channel-associated factor 2 (Tcaf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tcaf2 ( NM_146174.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666286.1 Mus musculus TRPM8 channel-associated factor 2 (Tcaf2), mRNA.

Price & Availability↑

CloneID	OMu18029
Clone ID Related Accession (Same CDS sequence)	NM_146174.1
Accession Version	NM_146174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	TRPM8 channel-associated factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC011487.1. Sequence Note: This sequence has been modified as follows: removed 39 bp suspected to be vector contamination from the 3' end. ##Evidence-Data-START## Transcript exon combination :: BC011487.1, AK131169.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849378 [ECO:0000348] ##Evidence-Data-END##

ATGGCAACAA CTCCCGATGC TGCTTTTGAG GCCCTCATGA ATGGAGTGAC CAGCTGGGAT 
CTCCCTAAAG AATTCACTCC CAGTGAACTC CTTCTTATTG GGGAGGCAGC CTTCCCAGTG 
ATGGTGAATG ACAAGGGCCA GGTGCTCATT GCTGTTTCTT TCTATGGCCA AGGCCGCCTG 
GTGGTCGTAT CCCATGAGAG CTACCTGATG CATGCTGGCT TGGCTCCTTT TCTTCTCAAT 
GCAGTGAGCT GGCTCTGCCC CTCCCCAGGG ACTCCCATTG AAGTGCATTC TTCCTTAGCA 
TCACTAGTTA ACATCCTACG TGGCTCTGGG ATAAATGCCT TGGTTCAGCC TGAGCCTGGA 
GAGGCTCTGG GAGTTTATTG CATTGATGCC TACAACGACA CCTTGACCAA GAAGCTGGTC 
CAGTTTGTGA AGCGTGGTGG AGGCTTGCTC ATTGGAGGTC AGGCCTGGAA TTGGGCCAGT 
CAGCATGGCA GTGACAAGGT GCTATTCAGT TTCCCTGGGA ACAAGGTGAC AAGTGTGGCT 
GGAGTCTACT TCACAGATGT CTATGGAGAT ATAAACCGGT TCAAAGTCTC TAAGAAGATA 
CCCAAGATCC CACTGTATAT CAGGTGTTGG GAGGAGCTCA GACATGACCA GGACCAGCTT 
CTGGATGGAA TCTCTATGTT GGATGTCAGG ACTGGGGGTG TCCCCTCACA GCTGTTGGTG 
CATGGGTCCC TGGCCTTCCC TCTGGGGCTC GATAACTCCT TCCTTAGCTG CTTCCTAGCA 
GCTGCTCACT ATGGCCGTGG TCGTGTGGTG TTGGCTGCAC ATGAGGCCAT GCTCTGCGCT 
CCTAAGATGG AACCCTTTTT GCTCAATGCT ATACGCTGGT TGTCCAGAGG TCAGGAAGAT 
AATATTGGGG TGAACACACG TCTCAAGAAT CTGAATTCCC TATTGTTGAA GCATGGCTTG 
AAATGCAGTC TGGAGTCCCA TTTGACCGAT GATATGTGTG TCTACTGCTG TGCAGCTTAT 
AGTGACCAGG AGGCCAAGAA AATACAGGAG TTTGTAGCAG AGGGTGGAGG GTTGCTCATT 
GGTGGTCAGT CCTGGTGGTG GGCTTCCCAG AACCCAGGCA GCTCTGCTTT AGGTAGTTTC 
CCTGGGAATG TCATCCTCAA TACTTTTGGC CTCAGCATTT TGCCTCGGAC AGTCAGCCCA 
GGCTGTTTTC CTATCCTTCA TATAGATATA AGAAACTATC ACTTTCGAGG GGCTCTTTCT 
GAGTTCCAAG CTATGTTGAA CCACAAGGAG GGGAACTTGG AAAAGAGGTA TTCGGGAAAG 
TTGGGAGTAG ATGGAGCAGG CTTTCTCCAG ATTCCTGCAC AGGGAGTTCC TGCTTACTTA 
TCTGTGCACC GGATCCTGAG GAAGATACTG CGACAGGCAG GCCTCCCAGC TGTGAGCAAG 
AGTAATCCAG TGTCCAGTCA CTCCTATGAG GCTGCGATAC TCCAGCTAGC CACAGAGCTG 
GCACACTCTG GGAGTGATTG CTCCCAGATA GCACATAACC TTAGCAGTCA GACCTGCTCC 
TCCAATCTCT CCTCCTCAGA ACACCCCATT ACCGTGGAGA TCAATGGAAC CAATCCAGGT 
GACCGTGATG TCTGGATGAG CACTGGGCTC TACCTCCTGG AAGGACAAAG CACCGAGATC 
TCTGTCTCTG AACCTGCTGC CTCTGCAGGT CTGAAGGTGC AGATTGGATG TCACACTGAT 
GACCTTACCT TTGCCATAAA GCTGTTTCGA GCCCCTGTGG TAACTTACCA GTGCTGTATG 
AACAGAACCC AACGGTCAGT CTCCTGCCTC TGGGGTGGTC TCCTGTACAT CATTGTGCCC 
AAAGGCTGTC AACTGGGCCC TGTGTCTGTT ACCATCACGA ATGCAGTGCC TGCCCCATAC 
TACAAGCTAG GTAAGACATC ACTGGAGGAG TGGAAGAGCT GCATCCAGAA GAACCTAGGC 
CCCTGGGGAG AGCTGGCCAC AGACAATGTC ATCCTGACAG TGCCAACTGC AAGCCTCAAG 
ACTCTGGAGA ATCCAGAGCC TCTGCTCCAA CTCTGGGATG AGATGATGCA GGCTGTGGCC 
AGGCTGGCAA GCCAGCCCTT CCCTTTCCAA AGACCTGAGA GAATTGTCGC TGATGTGCAG 
CTCTCAGCCG GCTGGATGCA TTCAGGATAC CCCATCATGT GCCACATGGA GTCTGTGCAA 
GAGCTAGTCA GTTTGGCAAA CATAAGAAGT AAGGGCCTGT GGGGACCCAT CCATGAGCTG 
GGCCACAATC AGCAGTGCAG GGGTTGGGAG TTCCCCCCAC ATACAACTGA GGCCACCTGC 
AATCTCTGGT CAGTCTATGT TCATGAGACC GTCCTGGGGA TTCCCAGGGC TCAGGCCCAC 
CCTCAACTGA AACCTGAAGA AAGAGAGAAG AGAATTAAAG AACACCTTCA GAAGGGAGCA 
CCCCTGCAAA ACTGGAATGT CTGGACAGCA CTGGAGACAT ATCTGCAGCT CCAAGAGGTC 
TTTGGGTGGG AACCGTTCAT CACCCTCTTT GCTGAATACC AAACCATTTT TTACATCCCT 
GAAGACAATG AGTGCAAGAT GAACATATGG CTGAAGTTGT TCTCTGAAAA AGTGCAGAAG 
AACCTGGTTC CTTTCTTTGA AGCCTGGGGC TGGCCTATCC AGAAGGATGT GGCTGAAGAC 
CTGGCCTGCT ATCCTTCCTG GGAGGATCAC CCCCTGAGGA TGTACATGGG GAGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666286.1
CDS	177..2936
Translation	MATTPDAAFEALMNGVTSWDLPKEFTPSELLLIGEAAFPVMVNDKGQVLIAVSFYGQGRLVVVSHESYLMHAGLAPFLLNAVSWLCPSPGTPIEVHSSLASLVNILRGSGINALVQPEPGEALGVYCIDAYNDTLTKKLVQFVKRGGGLLIGGQAWNWASQHGSDKVLFSFPGNKVTSVAGVYFTDVYGDINRFKVSKKIPKIPLYIRCWEELRHDQDQLLDGISMLDVRTGGVPSQLLVHGSLAFPLGLDNSFLSCFLAAAHYGRGRVVLAAHEAMLCAPKMEPFLLNAIRWLSRGQEDNIGVNTRLKNLNSLLLKHGLKCSLESHLTDDMCVYCCAAYSDQEAKKIQEFVAEGGGLLIGGQSWWWASQNPGSSALGSFPGNVILNTFGLSILPRTVSPGCFPILHIDIRNYHFRGALSEFQAMLNHKEGNLEKRYSGKLGVDGAGFLQIPAQGVPAYLSVHRILRKILRQAGLPAVSKSNPVSSHSYEAAILQLATELAHSGSDCSQIAHNLSSQTCSSNLSSSEHPITVEINGTNPGDRDVWMSTGLYLLEGQSTEISVSEPAASAGLKVQIGCHTDDLTFAIKLFRAPVVTYQCCMNRTQRSVSCLWGGLLYIIVPKGCQLGPVSVTITNAVPAPYYKLGKTSLEEWKSCIQKNLGPWGELATDNVILTVPTASLKTLENPEPLLQLWDEMMQAVARLASQPFPFQRPERIVADVQLSAGWMHSGYPIMCHMESVQELVSLANIRSKGLWGPIHELGHNQQCRGWEFPPHTTEATCNLWSVYVHETVLGIPRAQAHPQLKPEEREKRIKEHLQKGAPLQNWNVWTALETYLQLQEVFGWEPFITLFAEYQTIFYIPEDNECKMNIWLKLFSEKVQKNLVPFFEAWGWPIQKDVAEDLACYPSWEDHPLRMYMGSE

Target ORF information:

RefSeq Version	NM_146174.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TRPM8 channel-associated factor 2 (Tcaf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146174.1

ORF Insert Sequence:

ATGGCAACAA CTCCCGATGC TGCTTTTGAG GCCCTCATGA ATGGAGTGAC CAGCTGGGAT 
CTCCCTAAAG AATTCACTCC CAGTGAACTC CTTCTTATTG GGGAGGCAGC CTTCCCAGTG 
ATGGTGAATG ACAAGGGCCA GGTGCTCATT GCTGTTTCTT TCTATGGCCA AGGCCGCCTG 
GTGGTCGTAT CCCATGAGAG CTACCTGATG CATGCTGGCT TGGCTCCTTT TCTTCTCAAT 
GCAGTGAGCT GGCTCTGCCC CTCCCCAGGG ACTCCCATTG AAGTGCATTC TTCCTTAGCA 
TCACTAGTTA ACATCCTACG TGGCTCTGGG ATAAATGCCT TGGTTCAGCC TGAGCCTGGA 
GAGGCTCTGG GAGTTTATTG CATTGATGCC TACAACGACA CCTTGACCAA GAAGCTGGTC 
CAGTTTGTGA AGCGTGGTGG AGGCTTGCTC ATTGGAGGTC AGGCCTGGAA TTGGGCCAGT 
CAGCATGGCA GTGACAAGGT GCTATTCAGT TTCCCTGGGA ACAAGGTGAC AAGTGTGGCT 
GGAGTCTACT TCACAGATGT CTATGGAGAT ATAAACCGGT TCAAAGTCTC TAAGAAGATA 
CCCAAGATCC CACTGTATAT CAGGTGTTGG GAGGAGCTCA GACATGACCA GGACCAGCTT 
CTGGATGGAA TCTCTATGTT GGATGTCAGG ACTGGGGGTG TCCCCTCACA GCTGTTGGTG 
CATGGGTCCC TGGCCTTCCC TCTGGGGCTC GATAACTCCT TCCTTAGCTG CTTCCTAGCA 
GCTGCTCACT ATGGCCGTGG TCGTGTGGTG TTGGCTGCAC ATGAGGCCAT GCTCTGCGCT 
CCTAAGATGG AACCCTTTTT GCTCAATGCT ATACGCTGGT TGTCCAGAGG TCAGGAAGAT 
AATATTGGGG TGAACACACG TCTCAAGAAT CTGAATTCCC TATTGTTGAA GCATGGCTTG 
AAATGCAGTC TGGAGTCCCA TTTGACCGAT GATATGTGTG TCTACTGCTG TGCAGCTTAT 
AGTGACCAGG AGGCCAAGAA AATACAGGAG TTTGTAGCAG AGGGTGGAGG GTTGCTCATT 
GGTGGTCAGT CCTGGTGGTG GGCTTCCCAG AACCCAGGCA GCTCTGCTTT AGGTAGTTTC 
CCTGGGAATG TCATCCTCAA TACTTTTGGC CTCAGCATTT TGCCTCGGAC AGTCAGCCCA 
GGCTGTTTTC CTATCCTTCA TATAGATATA AGAAACTATC ACTTTCGAGG GGCTCTTTCT 
GAGTTCCAAG CTATGTTGAA CCACAAGGAG GGGAACTTGG AAAAGAGGTA TTCGGGAAAG 
TTGGGAGTAG ATGGAGCAGG CTTTCTCCAG ATTCCTGCAC AGGGAGTTCC TGCTTACTTA 
TCTGTGCACC GGATCCTGAG GAAGATACTG CGACAGGCAG GCCTCCCAGC TGTGAGCAAG 
AGTAATCCAG TGTCCAGTCA CTCCTATGAG GCTGCGATAC TCCAGCTAGC CACAGAGCTG 
GCACACTCTG GGAGTGATTG CTCCCAGATA GCACATAACC TTAGCAGTCA GACCTGCTCC 
TCCAATCTCT CCTCCTCAGA ACACCCCATT ACCGTGGAGA TCAATGGAAC CAATCCAGGT 
GACCGTGATG TCTGGATGAG CACTGGGCTC TACCTCCTGG AAGGACAAAG CACCGAGATC 
TCTGTCTCTG AACCTGCTGC CTCTGCAGGT CTGAAGGTGC AGATTGGATG TCACACTGAT 
GACCTTACCT TTGCCATAAA GCTGTTTCGA GCCCCTGTGG TAACTTACCA GTGCTGTATG 
AACAGAACCC AACGGTCAGT CTCCTGCCTC TGGGGTGGTC TCCTGTACAT CATTGTGCCC 
AAAGGCTGTC AACTGGGCCC TGTGTCTGTT ACCATCACGA ATGCAGTGCC TGCCCCATAC 
TACAAGCTAG GTAAGACATC ACTGGAGGAG TGGAAGAGCT GCATCCAGAA GAACCTAGGC 
CCCTGGGGAG AGCTGGCCAC AGACAATGTC ATCCTGACAG TGCCAACTGC AAGCCTCAAG 
ACTCTGGAGA ATCCAGAGCC TCTGCTCCAA CTCTGGGATG AGATGATGCA GGCTGTGGCC 
AGGCTGGCAA GCCAGCCCTT CCCTTTCCAA AGACCTGAGA GAATTGTCGC TGATGTGCAG 
CTCTCAGCCG GCTGGATGCA TTCAGGATAC CCCATCATGT GCCACATGGA GTCTGTGCAA 
GAGCTAGTCA GTTTGGCAAA CATAAGAAGT AAGGGCCTGT GGGGACCCAT CCATGAGCTG 
GGCCACAATC AGCAGTGCAG GGGTTGGGAG TTCCCCCCAC ATACAACTGA GGCCACCTGC 
AATCTCTGGT CAGTCTATGT TCATGAGACC GTCCTGGGGA TTCCCAGGGC TCAGGCCCAC 
CCTCAACTGA AACCTGAAGA AAGAGAGAAG AGAATTAAAG AACACCTTCA GAAGGGAGCA 
CCCCTGCAAA ACTGGAATGT CTGGACAGCA CTGGAGACAT ATCTGCAGCT CCAAGAGGTC 
TTTGGGTGGG AACCGTTCAT CACCCTCTTT GCTGAATACC AAACCATTTT TTACATCCCT 
GAAGACAATG AGTGCAAGAT GAACATATGG CTGAAGTTGT TCTCTGAAAA AGTGCAGAAG 
AACCTGGTTC CTTTCTTTGA AGCCTGGGGC TGGCCTATCC AGAAGGATGT GGCTGAAGAC 
CTGGCCTGCT ATCCTTCCTG GGAGGATCAC CCCCTGAGGA TGTACATGGG GAGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.
The Journal of cell biology208(1)89-107(2015 Jan)
Gkika D,Lemonnier L,Shapovalov G,Gordienko D,Poux C,Bernardini M,Bokhobza A,Bidaux G,Degerny C,Verreman K,Guarmit B,Benahmed M,de Launoit Y,Bindels RJ,Fiorio Pla A,Prevarskaya N

Haploinsufficiency of SAMD9L, an endosome fusion facilitator, causes myeloid malignancies in mice mimicking human diseases with monosomy 7.
Cancer cell24(3)305-17(2013 Sep)
Nagamachi A,Matsui H,Asou H,Ozaki Y,Aki D,Kanai A,Takubo K,Suda T,Nakamura T,Wolff L,Honda H,Inaba T

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Kitamura H,Nakagawa T,Nagase T,Ohara O,Koga H

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Tcaf2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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