tcaf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tcaf1
Entrez Gene ID	100145172
Full Name	TRPM8 channel associated factor 1
Synonyms	fam115,fam115a,fam139a
General protein information	Preferred Names TRPM8 channel associated factor 1 Names TRPM8 channel-associated factor homolog family with sequence similarity 115, member A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934560.4	XP_002934606.1	TRPM8 channel-associated factor homolog isoform X2
XM_031899109.1	XP_031754969.1	TRPM8 channel-associated factor homolog isoform X2
XM_004915986.4	XP_004916043.1	TRPM8 channel-associated factor homolog isoform X1
XM_031899108.1	XP_031754968.1	TRPM8 channel-associated factor homolog isoform X2
XM_004915986.3	XP_004916043.1	TRPM8 channel-associated factor homolog isoform X1
XM_002934560.5	XP_002934606.1	TRPM8 channel-associated factor homolog isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	FAM115C	NP_775949.2
Canis lupus familiaris (dog)	FAM115C	XP_539847.2
Xenopus tropicalis (tropical clawed frog)	LOC100485132	XP_002941113.2
Xenopus tropicalis (tropical clawed frog)	LOC100498358	XP_002934625.2
Mus musculus (house mouse)	Fam115c	NP_666286.1
Danio rerio (zebrafish)	LOC100535998	XP_003200030.1
Xenopus tropicalis (tropical clawed frog)	LOC100489234	XP_002943401.2
Pan troglodytes (chimpanzee)	FAM115C	XP_519453.3
	FAM115C	XP_001092570.2
Bos taurus (cattle)	FAM115C	NP_001095394.1
Xenopus tropicalis (tropical clawed frog)	fam115c	XP_002934627.2
Danio rerio (zebrafish)	LOC100535908	XP_003200029.1
Danio rerio (zebrafish)	zgc:162193	NP_001082796.1
Danio rerio (zebrafish)	LOC555689	XP_683371.5
Xenopus tropicalis (tropical clawed frog)	fam115a	XP_002934606.1

Gene Ontology

The following tcaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tcaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa12930	XM_004915986.4 Latest version!	Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa12931	XM_031899109.1 Latest version!	Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa12931	XM_002934560.5 Latest version!	Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa12931	XM_031899108.1 Latest version!	Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa12930	XM_004915986.3	Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa12931	XM_002934560.4	Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tcaf1 ( XM_004915986.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916043.1 Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa12930
Clone ID Related Accession (Same CDS sequence)	XM_004915986.4 , XM_004915986.3
Accession Version	XM_004915986.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TRPM8 channel-associated factor homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915986.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTGG AAGCCTCCCA TAAGACTCAA TGGCACTTCA ACCTCGCCCA AGGAAAATTT 
GGAATTATGG AGCTTTTTGA AGATTACAAA GCACTCACCA AAGATGTTGG TTCTCTAGAT 
TTCTCTGGAA GTTCTGTTCC ATGCAAGCTG CTAATAACAG GAGACATGGC ATTCCCTGTC 
CTGGTCACTC CTAGAAAGGA TGTGCTCATT GCTGCCTCTT ATTATGGAAA GGGGAAAATG 
GTGGTCATTG CTCATGAGAA TTATCTGAAT ATGAAAGAGT TTATGGTTTT CTTAAGGAAT 
TCTGTGACCT GGCTGAAACC AAACCCAAAA GCAGAAATTG GTGTAGATGG CAGCCTAACT 
CTACTAGCAG AGACACTTAC TGGCTCTGGC TGTCAAGTCC AAACCAGTTT AAGCCCTGCT 
GAGGGCTTGG GAGTGTTCTG TACAAGTGGA TATGATGATA GCCAGGCTCA GCAGATCATC 
TCATTTGTGC GAGAGGGGGG AGGCCTGCTG ATTGGGGCTC AGGCTTGGCA CTGGTCCTAC 
AGTCACAAGC AGGAAAATGT TCTCCACAAT TTTCCTGGAA ACAAAATAAC ATCAGTTTCT 
GGGGTGTACT TCACTGATAA TTATGGAGAA AAAGGAACAT TCTGTGTGAC TGATAATATG 
CCATGGACTC CATTATACAC AGACTTTGAC TTTTCAGCAG ATATGAAACA CCTTTTAAAT 
GGTGTATCGC AGCTCGATAT CAGCGGATAC AGCCTCCCCT CAGATCTTCT CATCTATGGG 
GCACTTGCAT TTCCAGTTGG TGTTGCTGAT AAGAAAGAAT GTTTTCTGGG TGCTGCACAC 
TATGGAAAAG GGCGTGTGGT TGTAGCATCA CATGACACCT ACATGTATAA GCCAGAGCTG 
AAAACCTTTA TCCTAAATAC AATATCATGG CTAGACACTG GAAAGAAAAA CAAAATTGGT 
ATTCATAAAG ACTTGGAAAA CCTTGCACAA TTGTTAGAGA AGGAAGGCAT TTTATGTACA 
ATATCCAGCT TTAGCCCTGA GCTGAGTGTA TATTGCTGTC CATGGTACAC CGAGGCTGAA 
GAATGTGCTA TCCATCAATT TGTTGCAGAA GGGGGAGGCC TATTTATTGG TGGCCAGTCC 
TGGAACTGGG CCTCCAAAAA CTCAGATCTA AATGTTGTTG CTGATTTCCC AGGCAACAAA 
ATACTGAACA AATTTGGCAT TAGCATCTTG GACTGGAGCA CCCCTCAAGG CAATTATAAA 
GCAGTCAATC CTGATGAAGC CACTAACCAG TATCGGTTCC CAAAGGCACT TTCACAGCTG 
CAAAACGAAC TAAAAAGTGA ACATGATCTG AAACCTCCCT TTAGCACCTG GATGTTCAAA 
CTAAGACGAG ATATCACCTA TTTCATGAGG CTCCCCGCCA GTCCTATCAT TTGCGCCATA 
CAGCGCCAGT TTATAGATAT GATGCAGACC TGTGAGCTAC CCAAGCTCAA TGGAAAAAAC 
TGTGTGAGCA GTTGCTCGAA AGAAGCTTTA ATCCTATCTT TGGCCCATGA AGCTTGCAAT 
GTGAACCAGG AGTGCAATGA GAAAAATATT ATGGAGAATG AGCCTGCAGT CACAATAGAA 
ATTGATGCCA CAAACCCAGG TTATGATGCA TGGAGAAGCA CCGGGCTCTA TTTGTCTCCC 
AAAAAGACAG CAGTGCTTGT GTTTCCTGCC TCAGCTGTTC AACAGGGTCT TCAGGTCCAG 
GTTGGCTGTC ACTCAGATGA TTTGGGCTCT AAAGATGAGT TTTATCGTGC GCCGATTGTT 
GTGCGGAAAA TTTCTGTTCA TAGAGAGAAG GTCCAGGTTT CCTGTTATTG GGGAGGACTC 
CTTTACATTA TTGTGAAAGA GAGGAGCAAC TTGGGTACAA TCCCAGTTCA AGTGTATGGA 
GCAGAACCAG CCCCAACCTA TATAAAAGGT AAGACCAGTC TTGACTCCTG GTTGCAATCA 
CTCCGTGACT TGCCTCCTCC ATGGGCTGAA TTGATTACAG AAAACATTAT CCTGACTGTA 
CCATCAGATG CTATCCGCTC ACTAAATGAT CCAGAAGAAC TACTTGGGTT TTGGGACAGA 
ATGATGGTAG CTATTACTGA GCTTGCTGCC ATACCGACAA CATTTTCTCG ACCAGAAAGA 
ATCGTAGCAG ATGTTCAGAT CTCAGCTGGT TGGATGCATG CTGGATACCC AGTCATGGTC 
CACTTAGCAT CAGTCAAATA TCTGACTAAC CTTAGCTGTA TGCAAAAGGA AGGTCTCTGG 
GGACCAATCC ATGAGCTTGG GCACAATCAA CAGCAAAAAG TTTGGGAGTT CCCCCCACAC 
ACTACAGAAG CCACTTGTAA CCTTTGGTCT GTATTTGTGA ATGAAACAGT TGTAGGAGTG 
CCCCGGGATA AAGCTAACTC TGCTCTCGAA CCAGCAAGCA GGGCTGCCTG TATTGAGGCA 
TATTTGAAAA CTGGACCCAA GTTAGAAAAT TGGAATGTAT GGACTGCACT GGAGACCTAT 
CTACAGTTGC AAGAAGGCTT TGGCTGGGAA CCATTCAAGC AGTTATTCAA AGACTACCAG 
AGCATGTCGG GCATCAGTAA TGACAACAAA TCAAAGATGA ACCTGTGGGC AGAGAAGTTC 
TCTGAGGCTG TGCAAACCAA TCTCATTCCA TTCTTTGAGG CCTGGGGATG GCCCATTGAG 
GAAGAAACTC GCAGTAAATT ATCTGCATTA CCTGTATGGG AGAAGGATCC CATGAAGCCA 
TATCTTAGTG CAATGAAGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916043.1
CDS	333..3116
Translation	MILEASHKTQWHFNLAQGKFGIMELFEDYKALTKDVGSLDFSGSSVPCKLLITGDMAFPVLVTPRKDVLIAASYYGKGKMVVIAHENYLNMKEFMVFLRNSVTWLKPNPKAEIGVDGSLTLLAETLTGSGCQVQTSLSPAEGLGVFCTSGYDDSQAQQIISFVREGGGLLIGAQAWHWSYSHKQENVLHNFPGNKITSVSGVYFTDNYGEKGTFCVTDNMPWTPLYTDFDFSADMKHLLNGVSQLDISGYSLPSDLLIYGALAFPVGVADKKECFLGAAHYGKGRVVVASHDTYMYKPELKTFILNTISWLDTGKKNKIGIHKDLENLAQLLEKEGILCTISSFSPELSVYCCPWYTEAEECAIHQFVAEGGGLFIGGQSWNWASKNSDLNVVADFPGNKILNKFGISILDWSTPQGNYKAVNPDEATNQYRFPKALSQLQNELKSEHDLKPPFSTWMFKLRRDITYFMRLPASPIICAIQRQFIDMMQTCELPKLNGKNCVSSCSKEALILSLAHEACNVNQECNEKNIMENEPAVTIEIDATNPGYDAWRSTGLYLSPKKTAVLVFPASAVQQGLQVQVGCHSDDLGSKDEFYRAPIVVRKISVHREKVQVSCYWGGLLYIIVKERSNLGTIPVQVYGAEPAPTYIKGKTSLDSWLQSLRDLPPPWAELITENIILTVPSDAIRSLNDPEELLGFWDRMMVAITELAAIPTTFSRPERIVADVQISAGWMHAGYPVMVHLASVKYLTNLSCMQKEGLWGPIHELGHNQQQKVWEFPPHTTEATCNLWSVFVNETVVGVPRDKANSALEPASRAACIEAYLKTGPKLENWNVWTALETYLQLQEGFGWEPFKQLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVWEKDPMKPYLSAMKS

Target ORF information:

RefSeq Version	XM_004915986.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915986.3

ORF Insert Sequence:

ATGATTTTGG AAGCCTCCCA TAAGACTCAA TGGCACTTCA ACCTCGCCCA AGGAAAATTT 
GGAATTATGG AGCTTTTTGA AGATTACAAA GCACTCACCA AAGATGTTGG TTCTCTAGAT 
TTCTCTGGAA GTTCTGTTCC ATGCAAGCTG CTAATAACAG GAGACATGGC ATTCCCTGTC 
CTGGTCACTC CTAGAAAGGA TGTGCTCATT GCTGCCTCTT ATTATGGAAA GGGGAAAATG 
GTGGTCATTG CTCATGAGAA TTATCTGAAT ATGAAAGAGT TTATGGTTTT CTTAAGGAAT 
TCTGTGACCT GGCTGAAACC AAACCCAAAA GCAGAAATTG GTGTAGATGG CAGCCTAACT 
CTACTAGCAG AGACACTTAC TGGCTCTGGC TGTCAAGTCC AAACCAGTTT AAGCCCTGCT 
GAGGGCTTGG GAGTGTTCTG TACAAGTGGA TATGATGATA GCCAGGCTCA GCAGATCATC 
TCATTTGTGC GAGAGGGGGG AGGCCTGCTG ATTGGGGCTC AGGCTTGGCA CTGGTCCTAC 
AGTCACAAGC AGGAAAATGT TCTCCACAAT TTTCCTGGAA ACAAAATAAC ATCAGTTTCT 
GGGGTGTACT TCACTGATAA TTATGGAGAA AAAGGAACAT TCTGTGTGAC TGATAATATG 
CCATGGACTC CATTATACAC AGACTTTGAC TTTTCAGCAG ATATGAAACA CCTTTTAAAT 
GGTGTATCGC AGCTCGATAT CAGCGGATAC AGCCTCCCCT CAGATCTTCT CATCTATGGG 
GCACTTGCAT TTCCAGTTGG TGTTGCTGAT AAGAAAGAAT GTTTTCTGGG TGCTGCACAC 
TATGGAAAAG GGCGTGTGGT TGTAGCATCA CATGACACCT ACATGTATAA GCCAGAGCTG 
AAAACCTTTA TCCTAAATAC AATATCATGG CTAGACACTG GAAAGAAAAA CAAAATTGGT 
ATTCATAAAG ACTTGGAAAA CCTTGCACAA TTGTTAGAGA AGGAAGGCAT TTTATGTACA 
ATATCCAGCT TTAGCCCTGA GCTGAGTGTA TATTGCTGTC CATGGTACAC CGAGGCTGAA 
GAATGTGCTA TCCATCAATT TGTTGCAGAA GGGGGAGGCC TATTTATTGG TGGCCAGTCC 
TGGAACTGGG CCTCCAAAAA CTCAGATCTA AATGTTGTTG CTGATTTCCC AGGCAACAAA 
ATACTGAACA AATTTGGCAT TAGCATCTTG GACTGGAGCA CCCCTCAAGG CAATTATAAA 
GCAGTCAATC CTGATGAAGC CACTAACCAG TATCGGTTCC CAAAGGCACT TTCACAGCTG 
CAAAACGAAC TAAAAAGTGA ACATGATCTG AAACCTCCCT TTAGCACCTG GATGTTCAAA 
CTAAGACGAG ATATCACCTA TTTCATGAGG CTCCCCGCCA GTCCTATCAT TTGCGCCATA 
CAGCGCCAGT TTATAGATAT GATGCAGACC TGTGAGCTAC CCAAGCTCAA TGGAAAAAAC 
TGTGTGAGCA GTTGCTCGAA AGAAGCTTTA ATCCTATCTT TGGCCCATGA AGCTTGCAAT 
GTGAACCAGG AGTGCAATGA GAAAAATATT ATGGAGAATG AGCCTGCAGT CACAATAGAA 
ATTGATGCCA CAAACCCAGG TTATGATGCA TGGAGAAGCA CCGGGCTCTA TTTGTCTCCC 
AAAAAGACAG CAGTGCTTGT GTTTCCTGCC TCAGCTGTTC AACAGGGTCT TCAGGTCCAG 
GTTGGCTGTC ACTCAGATGA TTTGGGCTCT AAAGATGAGT TTTATCGTGC GCCGATTGTT 
GTGCGGAAAA TTTCTGTTCA TAGAGAGAAG GTCCAGGTTT CCTGTTATTG GGGAGGACTC 
CTTTACATTA TTGTGAAAGA GAGGAGCAAC TTGGGTACAA TCCCAGTTCA AGTGTATGGA 
GCAGAACCAG CCCCAACCTA TATAAAAGGT AAGACCAGTC TTGACTCCTG GTTGCAATCA 
CTCCGTGACT TGCCTCCTCC ATGGGCTGAA TTGATTACAG AAAACATTAT CCTGACTGTA 
CCATCAGATG CTATCCGCTC ACTAAATGAT CCAGAAGAAC TACTTGGGTT TTGGGACAGA 
ATGATGGTAG CTATTACTGA GCTTGCTGCC ATACCGACAA CATTTTCTCG ACCAGAAAGA 
ATCGTAGCAG ATGTTCAGAT CTCAGCTGGT TGGATGCATG CTGGATACCC AGTCATGGTC 
CACTTAGCAT CAGTCAAATA TCTGACTAAC CTTAGCTGTA TGCAAAAGGA AGGTCTCTGG 
GGACCAATCC ATGAGCTTGG GCACAATCAA CAGCAAAAAG TTTGGGAGTT CCCCCCACAC 
ACTACAGAAG CCACTTGTAA CCTTTGGTCT GTATTTGTGA ATGAAACAGT TGTAGGAGTG 
CCCCGGGATA AAGCTAACTC TGCTCTCGAA CCAGCAAGCA GGGCTGCCTG TATTGAGGCA 
TATTTGAAAA CTGGACCCAA GTTAGAAAAT TGGAATGTAT GGACTGCACT GGAGACCTAT 
CTACAGTTGC AAGAAGGCTT TGGCTGGGAA CCATTCAAGC AGTTATTCAA AGACTACCAG 
AGCATGTCGG GCATCAGTAA TGACAACAAA TCAAAGATGA ACCTGTGGGC AGAGAAGTTC 
TCTGAGGCTG TGCAAACCAA TCTCATTCCA TTCTTTGAGG CCTGGGGATG GCCCATTGAG 
GAAGAAACTC GCAGTAAATT ATCTGCATTA CCTGTATGGG AGAAGGATCC CATGAAGCCA 
TATCTTAGTG CAATGAAGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcaf1 ( XM_002934560.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934606.1 Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa12931
Clone ID Related Accession (Same CDS sequence)	XM_002934560.4 , XM_031899109.1 , XM_031899108.1 , XM_002934560.5
Accession Version	XM_002934560.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TRPM8 channel-associated factor homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934560.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC 
ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC 
ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG 
ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA 
GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC 
TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT 
GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC 
AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG 
TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG 
ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA 
TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT 
GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA 
AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC 
TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT 
AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC 
AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT 
GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC 
TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG 
AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC 
AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC 
GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA 
CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC 
CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG 
AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC 
CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT 
GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG 
ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC 
TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG 
AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC 
ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA 
CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT 
GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA 
GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG 
GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA 
GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA 
GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA 
ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA 
GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG 
GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG 
AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG 
TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG 
TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG 
GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA 
ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT 
AGTGCAATGA AGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934606.1
CDS	302..3019
Translation	MELFEDYKALTKDVGSLDFSGSSVPCKLLITGDMAFPVLVTPRKDVLIAASYYGKGKMVVIAHENYLNMKEFMVFLRNSVTWLKPNPKAEIGVDGSLTLLAETLTGSGCQVQTSLSPAEGLGVFCTSGYDDSQAQQIISFVREGGGLLIGAQAWHWSYSHKQENVLHNFPGNKITSVSGVYFTDNYGEKGTFCVTDNMPWTPLYTDFDFSADMKHLLNGVSQLDISGYSLPSDLLIYGALAFPVGVADKKECFLGAAHYGKGRVVVASHDTYMYKPELKTFILNTISWLDTGKKNKIGIHKDLENLAQLLEKEGILCTISSFSPELSVYCCPWYTEAEECAIHQFVAEGGGLFIGGQSWNWASKNSDLNVVADFPGNKILNKFGISILDWSTPQGNYKAVNPDEATNQYRFPKALSQLQNELKSEHDLKPPFSTWMFKLRRDITYFMRLPASPIICAIQRQFIDMMQTCELPKLNGKNCVSSCSKEALILSLAHEACNVNQECNEKNIMENEPAVTIEIDATNPGYDAWRSTGLYLSPKKTAVLVFPASAVQQGLQVQVGCHSDDLGSKDEFYRAPIVVRKISVHREKVQVSCYWGGLLYIIVKERSNLGTIPVQVYGAEPAPTYIKGKTSLDSWLQSLRDLPPPWAELITENIILTVPSDAIRSLNDPEELLGFWDRMMVAITELAAIPTTFSRPERIVADVQISAGWMHAGYPVMVHLASVKYLTNLSCMQKEGLWGPIHELGHNQQQKVWEFPPHTTEATCNLWSVFVNETVVGVPRDKANSALEPASRAACIEAYLKTGPKLENWNVWTALETYLQLQEGFGWEPFKQLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVWEKDPMKPYLSAMKS

Target ORF information:

RefSeq Version	XM_002934560.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TRPM8 channel-associated factor 1 (tcaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934560.4

ORF Insert Sequence:

ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC 
ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC 
ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG 
ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA 
GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC 
TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT 
GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC 
AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG 
TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG 
ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA 
TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT 
GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA 
AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC 
TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT 
AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC 
AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT 
GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC 
TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG 
AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC 
AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC 
GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA 
CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC 
CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG 
AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC 
CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT 
GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG 
ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC 
TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG 
AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC 
ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA 
CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT 
GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA 
GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG 
GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA 
GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA 
GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA 
ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA 
GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG 
GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG 
AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG 
TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG 
TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG 
GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA 
ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT 
AGTGCAATGA AGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcaf1 ( XM_004915986.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916043.1 Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa12930
Clone ID Related Accession (Same CDS sequence)	XM_004915986.4 , XM_004915986.3
Accession Version	XM_004915986.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TRPM8 channel-associated factor homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915986.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTGG AAGCCTCCCA TAAGACTCAA TGGCACTTCA ACCTCGCCCA AGGAAAATTT 
GGAATTATGG AGCTTTTTGA AGATTACAAA GCACTCACCA AAGATGTTGG TTCTCTAGAT 
TTCTCTGGAA GTTCTGTTCC ATGCAAGCTG CTAATAACAG GAGACATGGC ATTCCCTGTC 
CTGGTCACTC CTAGAAAGGA TGTGCTCATT GCTGCCTCTT ATTATGGAAA GGGGAAAATG 
GTGGTCATTG CTCATGAGAA TTATCTGAAT ATGAAAGAGT TTATGGTTTT CTTAAGGAAT 
TCTGTGACCT GGCTGAAACC AAACCCAAAA GCAGAAATTG GTGTAGATGG CAGCCTAACT 
CTACTAGCAG AGACACTTAC TGGCTCTGGC TGTCAAGTCC AAACCAGTTT AAGCCCTGCT 
GAGGGCTTGG GAGTGTTCTG TACAAGTGGA TATGATGATA GCCAGGCTCA GCAGATCATC 
TCATTTGTGC GAGAGGGGGG AGGCCTGCTG ATTGGGGCTC AGGCTTGGCA CTGGTCCTAC 
AGTCACAAGC AGGAAAATGT TCTCCACAAT TTTCCTGGAA ACAAAATAAC ATCAGTTTCT 
GGGGTGTACT TCACTGATAA TTATGGAGAA AAAGGAACAT TCTGTGTGAC TGATAATATG 
CCATGGACTC CATTATACAC AGACTTTGAC TTTTCAGCAG ATATGAAACA CCTTTTAAAT 
GGTGTATCGC AGCTCGATAT CAGCGGATAC AGCCTCCCCT CAGATCTTCT CATCTATGGG 
GCACTTGCAT TTCCAGTTGG TGTTGCTGAT AAGAAAGAAT GTTTTCTGGG TGCTGCACAC 
TATGGAAAAG GGCGTGTGGT TGTAGCATCA CATGACACCT ACATGTATAA GCCAGAGCTG 
AAAACCTTTA TCCTAAATAC AATATCATGG CTAGACACTG GAAAGAAAAA CAAAATTGGT 
ATTCATAAAG ACTTGGAAAA CCTTGCACAA TTGTTAGAGA AGGAAGGCAT TTTATGTACA 
ATATCCAGCT TTAGCCCTGA GCTGAGTGTA TATTGCTGTC CATGGTACAC CGAGGCTGAA 
GAATGTGCTA TCCATCAATT TGTTGCAGAA GGGGGAGGCC TATTTATTGG TGGCCAGTCC 
TGGAACTGGG CCTCCAAAAA CTCAGATCTA AATGTTGTTG CTGATTTCCC AGGCAACAAA 
ATACTGAACA AATTTGGCAT TAGCATCTTG GACTGGAGCA CCCCTCAAGG CAATTATAAA 
GCAGTCAATC CTGATGAAGC CACTAACCAG TATCGGTTCC CAAAGGCACT TTCACAGCTG 
CAAAACGAAC TAAAAAGTGA ACATGATCTG AAACCTCCCT TTAGCACCTG GATGTTCAAA 
CTAAGACGAG ATATCACCTA TTTCATGAGG CTCCCCGCCA GTCCTATCAT TTGCGCCATA 
CAGCGCCAGT TTATAGATAT GATGCAGACC TGTGAGCTAC CCAAGCTCAA TGGAAAAAAC 
TGTGTGAGCA GTTGCTCGAA AGAAGCTTTA ATCCTATCTT TGGCCCATGA AGCTTGCAAT 
GTGAACCAGG AGTGCAATGA GAAAAATATT ATGGAGAATG AGCCTGCAGT CACAATAGAA 
ATTGATGCCA CAAACCCAGG TTATGATGCA TGGAGAAGCA CCGGGCTCTA TTTGTCTCCC 
AAAAAGACAG CAGTGCTTGT GTTTCCTGCC TCAGCTGTTC AACAGGGTCT TCAGGTCCAG 
GTTGGCTGTC ACTCAGATGA TTTGGGCTCT AAAGATGAGT TTTATCGTGC GCCGATTGTT 
GTGCGGAAAA TTTCTGTTCA TAGAGAGAAG GTCCAGGTTT CCTGTTATTG GGGAGGACTC 
CTTTACATTA TTGTGAAAGA GAGGAGCAAC TTGGGTACAA TCCCAGTTCA AGTGTATGGA 
GCAGAACCAG CCCCAACCTA TATAAAAGGT AAGACCAGTC TTGACTCCTG GTTGCAATCA 
CTCCGTGACT TGCCTCCTCC ATGGGCTGAA TTGATTACAG AAAACATTAT CCTGACTGTA 
CCATCAGATG CTATCCGCTC ACTAAATGAT CCAGAAGAAC TACTTGGGTT TTGGGACAGA 
ATGATGGTAG CTATTACTGA GCTTGCTGCC ATACCGACAA CATTTTCTCG ACCAGAAAGA 
ATCGTAGCAG ATGTTCAGAT CTCAGCTGGT TGGATGCATG CTGGATACCC AGTCATGGTC 
CACTTAGCAT CAGTCAAATA TCTGACTAAC CTTAGCTGTA TGCAAAAGGA AGGTCTCTGG 
GGACCAATCC ATGAGCTTGG GCACAATCAA CAGCAAAAAG TTTGGGAGTT CCCCCCACAC 
ACTACAGAAG CCACTTGTAA CCTTTGGTCT GTATTTGTGA ATGAAACAGT TGTAGGAGTG 
CCCCGGGATA AAGCTAACTC TGCTCTCGAA CCAGCAAGCA GGGCTGCCTG TATTGAGGCA 
TATTTGAAAA CTGGACCCAA GTTAGAAAAT TGGAATGTAT GGACTGCACT GGAGACCTAT 
CTACAGTTGC AAGAAGGCTT TGGCTGGGAA CCATTCAAGC AGTTATTCAA AGACTACCAG 
AGCATGTCGG GCATCAGTAA TGACAACAAA TCAAAGATGA ACCTGTGGGC AGAGAAGTTC 
TCTGAGGCTG TGCAAACCAA TCTCATTCCA TTCTTTGAGG CCTGGGGATG GCCCATTGAG 
GAAGAAACTC GCAGTAAATT ATCTGCATTA CCTGTATGGG AGAAGGATCC CATGAAGCCA 
TATCTTAGTG CAATGAAGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916043.1
CDS	391..3174
Translation	MILEASHKTQWHFNLAQGKFGIMELFEDYKALTKDVGSLDFSGSSVPCKLLITGDMAFPVLVTPRKDVLIAASYYGKGKMVVIAHENYLNMKEFMVFLRNSVTWLKPNPKAEIGVDGSLTLLAETLTGSGCQVQTSLSPAEGLGVFCTSGYDDSQAQQIISFVREGGGLLIGAQAWHWSYSHKQENVLHNFPGNKITSVSGVYFTDNYGEKGTFCVTDNMPWTPLYTDFDFSADMKHLLNGVSQLDISGYSLPSDLLIYGALAFPVGVADKKECFLGAAHYGKGRVVVASHDTYMYKPELKTFILNTISWLDTGKKNKIGIHKDLENLAQLLEKEGILCTISSFSPELSVYCCPWYTEAEECAIHQFVAEGGGLFIGGQSWNWASKNSDLNVVADFPGNKILNKFGISILDWSTPQGNYKAVNPDEATNQYRFPKALSQLQNELKSEHDLKPPFSTWMFKLRRDITYFMRLPASPIICAIQRQFIDMMQTCELPKLNGKNCVSSCSKEALILSLAHEACNVNQECNEKNIMENEPAVTIEIDATNPGYDAWRSTGLYLSPKKTAVLVFPASAVQQGLQVQVGCHSDDLGSKDEFYRAPIVVRKISVHREKVQVSCYWGGLLYIIVKERSNLGTIPVQVYGAEPAPTYIKGKTSLDSWLQSLRDLPPPWAELITENIILTVPSDAIRSLNDPEELLGFWDRMMVAITELAAIPTTFSRPERIVADVQISAGWMHAGYPVMVHLASVKYLTNLSCMQKEGLWGPIHELGHNQQQKVWEFPPHTTEATCNLWSVFVNETVVGVPRDKANSALEPASRAACIEAYLKTGPKLENWNVWTALETYLQLQEGFGWEPFKQLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVWEKDPMKPYLSAMKS

Target ORF information:

RefSeq Version	XM_004915986.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915986.4

ORF Insert Sequence:

ATGATTTTGG AAGCCTCCCA TAAGACTCAA TGGCACTTCA ACCTCGCCCA AGGAAAATTT 
GGAATTATGG AGCTTTTTGA AGATTACAAA GCACTCACCA AAGATGTTGG TTCTCTAGAT 
TTCTCTGGAA GTTCTGTTCC ATGCAAGCTG CTAATAACAG GAGACATGGC ATTCCCTGTC 
CTGGTCACTC CTAGAAAGGA TGTGCTCATT GCTGCCTCTT ATTATGGAAA GGGGAAAATG 
GTGGTCATTG CTCATGAGAA TTATCTGAAT ATGAAAGAGT TTATGGTTTT CTTAAGGAAT 
TCTGTGACCT GGCTGAAACC AAACCCAAAA GCAGAAATTG GTGTAGATGG CAGCCTAACT 
CTACTAGCAG AGACACTTAC TGGCTCTGGC TGTCAAGTCC AAACCAGTTT AAGCCCTGCT 
GAGGGCTTGG GAGTGTTCTG TACAAGTGGA TATGATGATA GCCAGGCTCA GCAGATCATC 
TCATTTGTGC GAGAGGGGGG AGGCCTGCTG ATTGGGGCTC AGGCTTGGCA CTGGTCCTAC 
AGTCACAAGC AGGAAAATGT TCTCCACAAT TTTCCTGGAA ACAAAATAAC ATCAGTTTCT 
GGGGTGTACT TCACTGATAA TTATGGAGAA AAAGGAACAT TCTGTGTGAC TGATAATATG 
CCATGGACTC CATTATACAC AGACTTTGAC TTTTCAGCAG ATATGAAACA CCTTTTAAAT 
GGTGTATCGC AGCTCGATAT CAGCGGATAC AGCCTCCCCT CAGATCTTCT CATCTATGGG 
GCACTTGCAT TTCCAGTTGG TGTTGCTGAT AAGAAAGAAT GTTTTCTGGG TGCTGCACAC 
TATGGAAAAG GGCGTGTGGT TGTAGCATCA CATGACACCT ACATGTATAA GCCAGAGCTG 
AAAACCTTTA TCCTAAATAC AATATCATGG CTAGACACTG GAAAGAAAAA CAAAATTGGT 
ATTCATAAAG ACTTGGAAAA CCTTGCACAA TTGTTAGAGA AGGAAGGCAT TTTATGTACA 
ATATCCAGCT TTAGCCCTGA GCTGAGTGTA TATTGCTGTC CATGGTACAC CGAGGCTGAA 
GAATGTGCTA TCCATCAATT TGTTGCAGAA GGGGGAGGCC TATTTATTGG TGGCCAGTCC 
TGGAACTGGG CCTCCAAAAA CTCAGATCTA AATGTTGTTG CTGATTTCCC AGGCAACAAA 
ATACTGAACA AATTTGGCAT TAGCATCTTG GACTGGAGCA CCCCTCAAGG CAATTATAAA 
GCAGTCAATC CTGATGAAGC CACTAACCAG TATCGGTTCC CAAAGGCACT TTCACAGCTG 
CAAAACGAAC TAAAAAGTGA ACATGATCTG AAACCTCCCT TTAGCACCTG GATGTTCAAA 
CTAAGACGAG ATATCACCTA TTTCATGAGG CTCCCCGCCA GTCCTATCAT TTGCGCCATA 
CAGCGCCAGT TTATAGATAT GATGCAGACC TGTGAGCTAC CCAAGCTCAA TGGAAAAAAC 
TGTGTGAGCA GTTGCTCGAA AGAAGCTTTA ATCCTATCTT TGGCCCATGA AGCTTGCAAT 
GTGAACCAGG AGTGCAATGA GAAAAATATT ATGGAGAATG AGCCTGCAGT CACAATAGAA 
ATTGATGCCA CAAACCCAGG TTATGATGCA TGGAGAAGCA CCGGGCTCTA TTTGTCTCCC 
AAAAAGACAG CAGTGCTTGT GTTTCCTGCC TCAGCTGTTC AACAGGGTCT TCAGGTCCAG 
GTTGGCTGTC ACTCAGATGA TTTGGGCTCT AAAGATGAGT TTTATCGTGC GCCGATTGTT 
GTGCGGAAAA TTTCTGTTCA TAGAGAGAAG GTCCAGGTTT CCTGTTATTG GGGAGGACTC 
CTTTACATTA TTGTGAAAGA GAGGAGCAAC TTGGGTACAA TCCCAGTTCA AGTGTATGGA 
GCAGAACCAG CCCCAACCTA TATAAAAGGT AAGACCAGTC TTGACTCCTG GTTGCAATCA 
CTCCGTGACT TGCCTCCTCC ATGGGCTGAA TTGATTACAG AAAACATTAT CCTGACTGTA 
CCATCAGATG CTATCCGCTC ACTAAATGAT CCAGAAGAAC TACTTGGGTT TTGGGACAGA 
ATGATGGTAG CTATTACTGA GCTTGCTGCC ATACCGACAA CATTTTCTCG ACCAGAAAGA 
ATCGTAGCAG ATGTTCAGAT CTCAGCTGGT TGGATGCATG CTGGATACCC AGTCATGGTC 
CACTTAGCAT CAGTCAAATA TCTGACTAAC CTTAGCTGTA TGCAAAAGGA AGGTCTCTGG 
GGACCAATCC ATGAGCTTGG GCACAATCAA CAGCAAAAAG TTTGGGAGTT CCCCCCACAC 
ACTACAGAAG CCACTTGTAA CCTTTGGTCT GTATTTGTGA ATGAAACAGT TGTAGGAGTG 
CCCCGGGATA AAGCTAACTC TGCTCTCGAA CCAGCAAGCA GGGCTGCCTG TATTGAGGCA 
TATTTGAAAA CTGGACCCAA GTTAGAAAAT TGGAATGTAT GGACTGCACT GGAGACCTAT 
CTACAGTTGC AAGAAGGCTT TGGCTGGGAA CCATTCAAGC AGTTATTCAA AGACTACCAG 
AGCATGTCGG GCATCAGTAA TGACAACAAA TCAAAGATGA ACCTGTGGGC AGAGAAGTTC 
TCTGAGGCTG TGCAAACCAA TCTCATTCCA TTCTTTGAGG CCTGGGGATG GCCCATTGAG 
GAAGAAACTC GCAGTAAATT ATCTGCATTA CCTGTATGGG AGAAGGATCC CATGAAGCCA 
TATCTTAGTG CAATGAAGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcaf1 ( XM_031899109.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754969.1 Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa12931
Clone ID Related Accession (Same CDS sequence)	XM_002934560.4 , XM_031899109.1 , XM_031899108.1 , XM_002934560.5
Accession Version	XM_031899109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TRPM8 channel-associated factor homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC 
ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC 
ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG 
ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA 
GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC 
TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT 
GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC 
AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG 
TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG 
ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA 
TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT 
GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA 
AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC 
TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT 
AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC 
AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT 
GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC 
TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG 
AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC 
AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC 
GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA 
CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC 
CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG 
AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC 
CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT 
GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG 
ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC 
TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG 
AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC 
ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA 
CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT 
GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA 
GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG 
GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA 
GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA 
GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA 
ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA 
GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG 
GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG 
AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG 
TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG 
TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG 
GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA 
ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT 
AGTGCAATGA AGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754969.1
CDS	31..2748
Translation	MELFEDYKALTKDVGSLDFSGSSVPCKLLITGDMAFPVLVTPRKDVLIAASYYGKGKMVVIAHENYLNMKEFMVFLRNSVTWLKPNPKAEIGVDGSLTLLAETLTGSGCQVQTSLSPAEGLGVFCTSGYDDSQAQQIISFVREGGGLLIGAQAWHWSYSHKQENVLHNFPGNKITSVSGVYFTDNYGEKGTFCVTDNMPWTPLYTDFDFSADMKHLLNGVSQLDISGYSLPSDLLIYGALAFPVGVADKKECFLGAAHYGKGRVVVASHDTYMYKPELKTFILNTISWLDTGKKNKIGIHKDLENLAQLLEKEGILCTISSFSPELSVYCCPWYTEAEECAIHQFVAEGGGLFIGGQSWNWASKNSDLNVVADFPGNKILNKFGISILDWSTPQGNYKAVNPDEATNQYRFPKALSQLQNELKSEHDLKPPFSTWMFKLRRDITYFMRLPASPIICAIQRQFIDMMQTCELPKLNGKNCVSSCSKEALILSLAHEACNVNQECNEKNIMENEPAVTIEIDATNPGYDAWRSTGLYLSPKKTAVLVFPASAVQQGLQVQVGCHSDDLGSKDEFYRAPIVVRKISVHREKVQVSCYWGGLLYIIVKERSNLGTIPVQVYGAEPAPTYIKGKTSLDSWLQSLRDLPPPWAELITENIILTVPSDAIRSLNDPEELLGFWDRMMVAITELAAIPTTFSRPERIVADVQISAGWMHAGYPVMVHLASVKYLTNLSCMQKEGLWGPIHELGHNQQQKVWEFPPHTTEATCNLWSVFVNETVVGVPRDKANSALEPASRAACIEAYLKTGPKLENWNVWTALETYLQLQEGFGWEPFKQLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVWEKDPMKPYLSAMKS

Target ORF information:

RefSeq Version	XM_031899109.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899109.1

ORF Insert Sequence:

ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC 
ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC 
ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG 
ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA 
GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC 
TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT 
GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC 
AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG 
TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG 
ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA 
TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT 
GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA 
AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC 
TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT 
AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC 
AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT 
GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC 
TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG 
AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC 
AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC 
GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA 
CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC 
CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG 
AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC 
CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT 
GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG 
ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC 
TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG 
AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC 
ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA 
CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT 
GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA 
GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG 
GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA 
GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA 
GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA 
ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA 
GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG 
GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG 
AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG 
TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG 
TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG 
GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA 
ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT 
AGTGCAATGA AGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcaf1 ( XM_031899108.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754968.1 Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa12931
Clone ID Related Accession (Same CDS sequence)	XM_002934560.4 , XM_031899109.1 , XM_031899108.1 , XM_002934560.5
Accession Version	XM_031899108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TRPM8 channel-associated factor homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC 
ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC 
ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG 
ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA 
GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC 
TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT 
GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC 
AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG 
TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG 
ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA 
TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT 
GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA 
AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC 
TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT 
AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC 
AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT 
GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC 
TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG 
AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC 
AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC 
GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA 
CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC 
CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG 
AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC 
CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT 
GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG 
ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC 
TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG 
AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC 
ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA 
CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT 
GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA 
GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG 
GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA 
GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA 
GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA 
ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA 
GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG 
GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG 
AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG 
TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG 
TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG 
GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA 
ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT 
AGTGCAATGA AGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754968.1
CDS	107..2824
Translation	MELFEDYKALTKDVGSLDFSGSSVPCKLLITGDMAFPVLVTPRKDVLIAASYYGKGKMVVIAHENYLNMKEFMVFLRNSVTWLKPNPKAEIGVDGSLTLLAETLTGSGCQVQTSLSPAEGLGVFCTSGYDDSQAQQIISFVREGGGLLIGAQAWHWSYSHKQENVLHNFPGNKITSVSGVYFTDNYGEKGTFCVTDNMPWTPLYTDFDFSADMKHLLNGVSQLDISGYSLPSDLLIYGALAFPVGVADKKECFLGAAHYGKGRVVVASHDTYMYKPELKTFILNTISWLDTGKKNKIGIHKDLENLAQLLEKEGILCTISSFSPELSVYCCPWYTEAEECAIHQFVAEGGGLFIGGQSWNWASKNSDLNVVADFPGNKILNKFGISILDWSTPQGNYKAVNPDEATNQYRFPKALSQLQNELKSEHDLKPPFSTWMFKLRRDITYFMRLPASPIICAIQRQFIDMMQTCELPKLNGKNCVSSCSKEALILSLAHEACNVNQECNEKNIMENEPAVTIEIDATNPGYDAWRSTGLYLSPKKTAVLVFPASAVQQGLQVQVGCHSDDLGSKDEFYRAPIVVRKISVHREKVQVSCYWGGLLYIIVKERSNLGTIPVQVYGAEPAPTYIKGKTSLDSWLQSLRDLPPPWAELITENIILTVPSDAIRSLNDPEELLGFWDRMMVAITELAAIPTTFSRPERIVADVQISAGWMHAGYPVMVHLASVKYLTNLSCMQKEGLWGPIHELGHNQQQKVWEFPPHTTEATCNLWSVFVNETVVGVPRDKANSALEPASRAACIEAYLKTGPKLENWNVWTALETYLQLQEGFGWEPFKQLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVWEKDPMKPYLSAMKS

Target ORF information:

RefSeq Version	XM_031899108.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899108.1

ORF Insert Sequence:

ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC 
ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC 
ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG 
ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA 
GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC 
TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT 
GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC 
AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG 
TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG 
ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA 
TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT 
GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA 
AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC 
TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT 
AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC 
AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT 
GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC 
TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG 
AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC 
AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC 
GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA 
CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC 
CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG 
AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC 
CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT 
GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG 
ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC 
TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG 
AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC 
ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA 
CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT 
GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA 
GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG 
GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA 
GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA 
GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA 
ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA 
GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG 
GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG 
AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG 
TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG 
TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG 
GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA 
ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT 
AGTGCAATGA AGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcaf1 ( XM_002934560.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934606.1 Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa12931
Clone ID Related Accession (Same CDS sequence)	XM_002934560.4 , XM_031899109.1 , XM_031899108.1 , XM_002934560.5
Accession Version	XM_002934560.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TRPM8 channel-associated factor homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934560.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC 
ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC 
ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG 
ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA 
GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC 
TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT 
GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC 
AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG 
TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG 
ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA 
TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT 
GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA 
AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC 
TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT 
AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC 
AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT 
GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC 
TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG 
AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC 
AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC 
GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA 
CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC 
CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG 
AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC 
CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT 
GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG 
ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC 
TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG 
AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC 
ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA 
CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT 
GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA 
GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG 
GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA 
GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA 
GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA 
ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA 
GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG 
GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG 
AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG 
TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG 
TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG 
GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA 
ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT 
AGTGCAATGA AGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934606.1
CDS	360..3077
Translation	MELFEDYKALTKDVGSLDFSGSSVPCKLLITGDMAFPVLVTPRKDVLIAASYYGKGKMVVIAHENYLNMKEFMVFLRNSVTWLKPNPKAEIGVDGSLTLLAETLTGSGCQVQTSLSPAEGLGVFCTSGYDDSQAQQIISFVREGGGLLIGAQAWHWSYSHKQENVLHNFPGNKITSVSGVYFTDNYGEKGTFCVTDNMPWTPLYTDFDFSADMKHLLNGVSQLDISGYSLPSDLLIYGALAFPVGVADKKECFLGAAHYGKGRVVVASHDTYMYKPELKTFILNTISWLDTGKKNKIGIHKDLENLAQLLEKEGILCTISSFSPELSVYCCPWYTEAEECAIHQFVAEGGGLFIGGQSWNWASKNSDLNVVADFPGNKILNKFGISILDWSTPQGNYKAVNPDEATNQYRFPKALSQLQNELKSEHDLKPPFSTWMFKLRRDITYFMRLPASPIICAIQRQFIDMMQTCELPKLNGKNCVSSCSKEALILSLAHEACNVNQECNEKNIMENEPAVTIEIDATNPGYDAWRSTGLYLSPKKTAVLVFPASAVQQGLQVQVGCHSDDLGSKDEFYRAPIVVRKISVHREKVQVSCYWGGLLYIIVKERSNLGTIPVQVYGAEPAPTYIKGKTSLDSWLQSLRDLPPPWAELITENIILTVPSDAIRSLNDPEELLGFWDRMMVAITELAAIPTTFSRPERIVADVQISAGWMHAGYPVMVHLASVKYLTNLSCMQKEGLWGPIHELGHNQQQKVWEFPPHTTEATCNLWSVFVNETVVGVPRDKANSALEPASRAACIEAYLKTGPKLENWNVWTALETYLQLQEGFGWEPFKQLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVWEKDPMKPYLSAMKS

Target ORF information:

RefSeq Version	XM_002934560.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TRPM8 channel associated factor 1 (tcaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934560.5

ORF Insert Sequence:

ATGGAGCTTT TTGAAGATTA CAAAGCACTC ACCAAAGATG TTGGTTCTCT AGATTTCTCT 
GGAAGTTCTG TTCCATGCAA GCTGCTAATA ACAGGAGACA TGGCATTCCC TGTCCTGGTC 
ACTCCTAGAA AGGATGTGCT CATTGCTGCC TCTTATTATG GAAAGGGGAA AATGGTGGTC 
ATTGCTCATG AGAATTATCT GAATATGAAA GAGTTTATGG TTTTCTTAAG GAATTCTGTG 
ACCTGGCTGA AACCAAACCC AAAAGCAGAA ATTGGTGTAG ATGGCAGCCT AACTCTACTA 
GCAGAGACAC TTACTGGCTC TGGCTGTCAA GTCCAAACCA GTTTAAGCCC TGCTGAGGGC 
TTGGGAGTGT TCTGTACAAG TGGATATGAT GATAGCCAGG CTCAGCAGAT CATCTCATTT 
GTGCGAGAGG GGGGAGGCCT GCTGATTGGG GCTCAGGCTT GGCACTGGTC CTACAGTCAC 
AAGCAGGAAA ATGTTCTCCA CAATTTTCCT GGAAACAAAA TAACATCAGT TTCTGGGGTG 
TACTTCACTG ATAATTATGG AGAAAAAGGA ACATTCTGTG TGACTGATAA TATGCCATGG 
ACTCCATTAT ACACAGACTT TGACTTTTCA GCAGATATGA AACACCTTTT AAATGGTGTA 
TCGCAGCTCG ATATCAGCGG ATACAGCCTC CCCTCAGATC TTCTCATCTA TGGGGCACTT 
GCATTTCCAG TTGGTGTTGC TGATAAGAAA GAATGTTTTC TGGGTGCTGC ACACTATGGA 
AAAGGGCGTG TGGTTGTAGC ATCACATGAC ACCTACATGT ATAAGCCAGA GCTGAAAACC 
TTTATCCTAA ATACAATATC ATGGCTAGAC ACTGGAAAGA AAAACAAAAT TGGTATTCAT 
AAAGACTTGG AAAACCTTGC ACAATTGTTA GAGAAGGAAG GCATTTTATG TACAATATCC 
AGCTTTAGCC CTGAGCTGAG TGTATATTGC TGTCCATGGT ACACCGAGGC TGAAGAATGT 
GCTATCCATC AATTTGTTGC AGAAGGGGGA GGCCTATTTA TTGGTGGCCA GTCCTGGAAC 
TGGGCCTCCA AAAACTCAGA TCTAAATGTT GTTGCTGATT TCCCAGGCAA CAAAATACTG 
AACAAATTTG GCATTAGCAT CTTGGACTGG AGCACCCCTC AAGGCAATTA TAAAGCAGTC 
AATCCTGATG AAGCCACTAA CCAGTATCGG TTCCCAAAGG CACTTTCACA GCTGCAAAAC 
GAACTAAAAA GTGAACATGA TCTGAAACCT CCCTTTAGCA CCTGGATGTT CAAACTAAGA 
CGAGATATCA CCTATTTCAT GAGGCTCCCC GCCAGTCCTA TCATTTGCGC CATACAGCGC 
CAGTTTATAG ATATGATGCA GACCTGTGAG CTACCCAAGC TCAATGGAAA AAACTGTGTG 
AGCAGTTGCT CGAAAGAAGC TTTAATCCTA TCTTTGGCCC ATGAAGCTTG CAATGTGAAC 
CAGGAGTGCA ATGAGAAAAA TATTATGGAG AATGAGCCTG CAGTCACAAT AGAAATTGAT 
GCCACAAACC CAGGTTATGA TGCATGGAGA AGCACCGGGC TCTATTTGTC TCCCAAAAAG 
ACAGCAGTGC TTGTGTTTCC TGCCTCAGCT GTTCAACAGG GTCTTCAGGT CCAGGTTGGC 
TGTCACTCAG ATGATTTGGG CTCTAAAGAT GAGTTTTATC GTGCGCCGAT TGTTGTGCGG 
AAAATTTCTG TTCATAGAGA GAAGGTCCAG GTTTCCTGTT ATTGGGGAGG ACTCCTTTAC 
ATTATTGTGA AAGAGAGGAG CAACTTGGGT ACAATCCCAG TTCAAGTGTA TGGAGCAGAA 
CCAGCCCCAA CCTATATAAA AGGTAAGACC AGTCTTGACT CCTGGTTGCA ATCACTCCGT 
GACTTGCCTC CTCCATGGGC TGAATTGATT ACAGAAAACA TTATCCTGAC TGTACCATCA 
GATGCTATCC GCTCACTAAA TGATCCAGAA GAACTACTTG GGTTTTGGGA CAGAATGATG 
GTAGCTATTA CTGAGCTTGC TGCCATACCG ACAACATTTT CTCGACCAGA AAGAATCGTA 
GCAGATGTTC AGATCTCAGC TGGTTGGATG CATGCTGGAT ACCCAGTCAT GGTCCACTTA 
GCATCAGTCA AATATCTGAC TAACCTTAGC TGTATGCAAA AGGAAGGTCT CTGGGGACCA 
ATCCATGAGC TTGGGCACAA TCAACAGCAA AAAGTTTGGG AGTTCCCCCC ACACACTACA 
GAAGCCACTT GTAACCTTTG GTCTGTATTT GTGAATGAAA CAGTTGTAGG AGTGCCCCGG 
GATAAAGCTA ACTCTGCTCT CGAACCAGCA AGCAGGGCTG CCTGTATTGA GGCATATTTG 
AAAACTGGAC CCAAGTTAGA AAATTGGAAT GTATGGACTG CACTGGAGAC CTATCTACAG 
TTGCAAGAAG GCTTTGGCTG GGAACCATTC AAGCAGTTAT TCAAAGACTA CCAGAGCATG 
TCGGGCATCA GTAATGACAA CAAATCAAAG ATGAACCTGT GGGCAGAGAA GTTCTCTGAG 
GCTGTGCAAA CCAATCTCAT TCCATTCTTT GAGGCCTGGG GATGGCCCAT TGAGGAAGAA 
ACTCGCAGTA AATTATCTGC ATTACCTGTA TGGGAGAAGG ATCCCATGAA GCCATATCTT 
AGTGCAATGA AGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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tcaf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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