TCAF2 cDNA ORF clone, Bos taurus(cattle)

The following TCAF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCAF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa44221 NM_001101924.1
Latest version!
Bos taurus TRPM8 channel associated factor 2 (TCAF2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18848 XM_010804547.3
Latest version!
Bos taurus TRPM8 channel associated factor 2 (TCAF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18848 XM_005205821.4
Latest version!
Bos taurus TRPM8 channel associated factor 2 (TCAF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18848 XM_005205822.4
Latest version!
Bos taurus TRPM8 channel associated factor 2 (TCAF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18848 XM_005205823.4
Latest version!
Bos taurus TRPM8 channel associated factor 2 (TCAF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
OBa18848 XM_010804547.2 Bos taurus TRPM8 channel-associated factor 2 (TCAF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18848 XM_005205821.3 Bos taurus TRPM8 channel-associated factor 2 (TCAF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18848 XM_005205823.3 Bos taurus TRPM8 channel-associated factor 2 (TCAF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18848 XM_005205822.3 Bos taurus TRPM8 channel-associated factor 2 (TCAF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa44221
    Clone ID Related Accession (Same CDS sequence) NM_001101924.1
    Accession Version NM_001101924.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-10
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC148091.1. On Aug 21, 2007 this sequence version replaced XM_587449.3. ##Evidence-Data-START## Transcript exon combination :: BC148091.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCATGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTAACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GATGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095394.1
    CDS88..2832
    Translation

    Target ORF information:

    RefSeq Version NM_001101924.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 2 (TCAF2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001101924.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCATGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTAACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GATGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18848
    Clone ID Related Accession (Same CDS sequence) XM_005205823.3 , XM_005205822.3 , XM_010804547.3 , XM_010804547.2 , XM_005205821.3 , XM_005205821.4 , XM_005205823.4 , XM_005205822.4
    Accession Version XM_005205823.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205880.1
    CDS95..2839
    Translation

    Target ORF information:

    RefSeq Version XM_005205823.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel-associated factor 2 (TCAF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205823.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18848
    Clone ID Related Accession (Same CDS sequence) XM_005205823.3 , XM_005205822.3 , XM_010804547.3 , XM_010804547.2 , XM_005205821.3 , XM_005205821.4 , XM_005205823.4 , XM_005205822.4
    Accession Version XM_005205822.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205879.1
    CDS221..2965
    Translation

    Target ORF information:

    RefSeq Version XM_005205822.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel-associated factor 2 (TCAF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205822.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18848
    Clone ID Related Accession (Same CDS sequence) XM_005205823.3 , XM_005205822.3 , XM_010804547.3 , XM_010804547.2 , XM_005205821.3 , XM_005205821.4 , XM_005205823.4 , XM_005205822.4
    Accession Version XM_010804547.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010804547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802849.1
    CDS134..2878
    Translation

    Target ORF information:

    RefSeq Version XM_010804547.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 2 (TCAF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804547.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18848
    Clone ID Related Accession (Same CDS sequence) XM_005205823.3 , XM_005205822.3 , XM_010804547.3 , XM_010804547.2 , XM_005205821.3 , XM_005205821.4 , XM_005205823.4 , XM_005205822.4
    Accession Version XM_010804547.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802849.1
    CDS182..2926
    Translation

    Target ORF information:

    RefSeq Version XM_010804547.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel-associated factor 2 (TCAF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804547.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18848
    Clone ID Related Accession (Same CDS sequence) XM_005205823.3 , XM_005205822.3 , XM_010804547.3 , XM_010804547.2 , XM_005205821.3 , XM_005205821.4 , XM_005205823.4 , XM_005205822.4
    Accession Version XM_005205821.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205821.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205878.1
    CDS129..2873
    Translation

    Target ORF information:

    RefSeq Version XM_005205821.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel-associated factor 2 (TCAF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205821.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18848
    Clone ID Related Accession (Same CDS sequence) XM_005205823.3 , XM_005205822.3 , XM_010804547.3 , XM_010804547.2 , XM_005205821.3 , XM_005205821.4 , XM_005205823.4 , XM_005205822.4
    Accession Version XM_005205821.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205821.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205878.1
    CDS138..2882
    Translation

    Target ORF information:

    RefSeq Version XM_005205821.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 2 (TCAF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205821.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18848
    Clone ID Related Accession (Same CDS sequence) XM_005205823.3 , XM_005205822.3 , XM_010804547.3 , XM_010804547.2 , XM_005205821.3 , XM_005205821.4 , XM_005205823.4 , XM_005205822.4
    Accession Version XM_005205823.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205823.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205880.1
    CDS147..2891
    Translation

    Target ORF information:

    RefSeq Version XM_005205823.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 2 (TCAF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205823.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18848
    Clone ID Related Accession (Same CDS sequence) XM_005205823.3 , XM_005205822.3 , XM_010804547.3 , XM_010804547.2 , XM_005205821.3 , XM_005205821.4 , XM_005205823.4 , XM_005205822.4
    Accession Version XM_005205822.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205822.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205879.1
    CDS188..2932
    Translation

    Target ORF information:

    RefSeq Version XM_005205822.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 2 (TCAF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205822.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACAA CTCCTGCGGC TGCCTTTGAG GCCCTCATGG ATGGAGTGAC GAGCTGGGAG 
    CTACCCGAAG GCCCTGTTCC CAGTGAACTC CTTCTTACTG GAGAGGCTGC CTTCCCAGTG
    ATGGTGAACG ATAAAGGCCA GGTCCTCATT GCGGCCTCCT TCTATGGCCG AGGCCGCTTG
    GTGGTGGTGT CCCACGAGGG CTATCTGCTG GATGCCGGCC TGGCCCGGTT TCTTCTCAAT
    GCAGTGCGCT GGCTCTCTCC CTCTCCTGGG GCGCCTGTTG GTGTGCACCC ATCCCTGGCA
    TCACTAGCAC ACATCCTGGA GGGCTCCGGG GTGGAGGCAC AGGTTCACCC AGAACCAGCA
    GAACCCCTGG GGGTTTACTG CATCAGTGCC TACAATGACA CCATGACTGC GGAACTGATC
    CAGTTTGTGA AACGTGGTGG GGGCTTGCTC ATCGGGGGCC AGGCCTGGCA CTGGGCCAGT
    CAGCACGGTA GTGACCAGGT GCTGTCCGAG TTCCCAGGGA ACCAGGTGAC CAGTGTGGCC
    GGAGTGTACT TCACTGACAC CTACGGGGTC AAAGGCCGCT TCAAGGTCTC CAAGAAGGTG
    CCCAAGATCC CTCTCCAGGT CAGGTGTGGG GAGGACCTCA GGCAGGATCA GCAGCAGCTC
    CTGGAAGGGA TCTCGGAGCT CGACATCGGG ACGAAGGGAC TCCCCTCGCA GCTGCTGGTG
    CACGGGGCCC TGGCCTTCCC CCTGGGCTTA GATGCCTCTC TCCGCTGCTT CCTGGCAGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTCCTG GCTGCCCATG AGGGCATGCT CTCTGCTCCC
    AGTTTGGGAC CCTTTTTACT CAATGCTGTG CGCTGGCTGG CCAAAGGCCA GACAGGCAAA
    GTTGGGGTGA ACACCAGTCT AGAAAAACTG CACACCCTGC TGTTGGAGCA TGGTCTTGAG
    TGCAGTCTTG AGCCCCATCT GACAAGTGGC GTGTGCGTCT ACTGCTGTAC AGCTTACAGC
    GACAAGGAGG CTAAGCAGCT GCAGGAGTTC GTGGCCGAGG GGGGTGGCTT GCTGATCGGG
    GGCCACGCCT GGTGGTGGGC CTCCCAGAAC CCCGGCCGCT CCGCGTTGGC TGATTTCCCC
    GGTAACGTCA TACTGAACAG CTTTGGCCTC AGCATCCTAC CTTGGACCCT GGACCCGGGC
    TGTTTCCCGG TCCCTTCCGC CGACAGCCTG AACTACCACT TCCGCAAGGC GCTGTCTGAA
    TTCCAGGCTA CCTTGAACCT CGAGGGTGGC AACTTGGAGA AGAACTGGCT GGCGAAACTG
    AGAGTGGATG GAGCCGCCTT CCTCCAGATT CCCGCAGAGG GGGTCCCTGC CTATGCCTCC
    CTGCACCGGC TCCTGAGGAA GCAGCTGCGG CTGCGTCTTT CGGGGTTCCC AGCTGTGAGC
    CGGGAGAACC CGGTGGCCGG TGATTCCTGT GAGGCGGTGG TGCTCTGCCT GGCCACAGAG
    CTGGCGCGCT CTGGCACTGA CTGCTCCGAG CTGGCACAGG GGCTTGGAGC CTGGTCCTGC
    AGCTCCAATC TGTGCCCATC AGAACACACA GTGGAGATCA ATGCACGCAA CCCAAGCGAT
    GATGCCTGGA TGAGTACAGG GCTTTACCTC CCAAACGGAC AACTTACAGA AGTCTGCTTG
    TGTGAAGCTG CAGCCTGTGC CGGCCTGAAG TTACAGATTG GCTGCCACAC CGACAACTTG
    ATGAGTGCCA GCAAGCTGTC TCGAGCCCCT GTGGTGACTC ACCAGTGCCA TATGGACAGG
    ACCGAACAGT TGGTCTCCAA CCTCTGGGGC GGCCTTCTCT ACGTCATCGT GCCCACGGGC
    TGTAACCTGG GGCCCATGTC CATCACCATC AAGAGGGCTG TGCCTGCCCC GTATTACAAG
    CTGGGTGAGA CATCCCTGGA GGCGTGGAGG AGCTGTATTC AGGAGAGCCC GGCTCCCTGG
    GGAGAGCTGG CGACAGACAA CATCATCTTG ACGGTGCCGA CTGCAGACCT GCGGGCCCTG
    GAGGACCCAG AGCCCTTGCT CCGCCTCTGG GATGAGATGA TGGAGGCTAT AGCCAGGCTG
    GCGGCCCAGC CCTTCCCTTT CCGCCGTCCA GAGAGGATTG TTGCTGATGT GCAGATCTCA
    GCCGGCTGGA TGCACTCGGG CTACCCCATC ATGTGCCACC TGGAGTCAGT GTCGGAGCTC
    ATCGATGAGA CGGGCATGAG AAGCCGCGGG CTCTGGGGCC CCGTGCACGA GCTGGGCCAC
    AACCAGCAGC GGGAGCAGTG GGAGTTCCCC CCGCACACCA CCGAGGCCAC CTGCAACCTG
    TGGTCGGTGT ATGTGCACGA GACGGTCCTG GGCATCCCCA GGGCTCAGGC CCACCCCGCT
    CTGAGCCCTC CAGAACGGGA GAATAGGATC AAAACACACC TGGAGAAGGG AGCCCCCCTG
    TGCGACTGGA AAGTGTGGAC AGCTCTGGAA ACATATCTGC AGCTCCAGGA GGCCTTTGGG
    TGGGAGCCGT TCACCCAGCT CTTTGCTGAG TACCAGACCC TCTCTGACAT CCCCAATGAC
    AACCCTGGCA AGATGAACCT GTGGGTGAGG AAGTTCTCTG AAAAGGTGCA GAAGAACCTG
    GCTCCGTTCT TTGAGGCCTG GGGCTGGCCC GTCGAGAAGG AAGTAGCTTC TAGCCTGGCC
    TGTCTGCCTG AGTGGGAAGA AAATCCCATG AGGATGTACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.