RALGAPA1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010824903.2	XP_010823205.1	ral GTPase-activating protein subunit alpha-1 isoform X2
XM_003583756.4	XP_003583804.1	ral GTPase-activating protein subunit alpha-1 isoform X7
XM_005222080.3	XP_005222137.1	ral GTPase-activating protein subunit alpha-1 isoform X8
XM_010817180.3	XP_010815482.1	ral GTPase-activating protein subunit alpha-1 isoform X2
XM_015459295.2	XP_015314781.1	ral GTPase-activating protein subunit alpha-1 isoform X5
XM_015468546.1	XP_015324032.1	ral GTPase-activating protein subunit alpha-1 isoform X5
XM_010824902.2	XP_010823204.1	ral GTPase-activating protein subunit alpha-1 isoform X1
XM_010824904.2	XP_010823206.1	ral GTPase-activating protein subunit alpha-1 isoform X3
XM_010824906.2	XP_010823208.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_005222076.4	XP_005222133.1	ral GTPase-activating protein subunit alpha-1 isoform X6
XM_005222080.4	XP_005222137.1	ral GTPase-activating protein subunit alpha-1 isoform X8
XM_010817181.2	XP_010815483.1	ral GTPase-activating protein subunit alpha-1 isoform X3
XM_010817183.2	XP_010815485.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_010817179.3	XP_010815481.1	ral GTPase-activating protein subunit alpha-1 isoform X1
XM_010817180.2	XP_010815482.1	ral GTPase-activating protein subunit alpha-1 isoform X2
XM_010817182.2	XP_010815484.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_015459295.1	XP_015314781.1	ral GTPase-activating protein subunit alpha-1 isoform X5
XM_010824905.2	XP_010823207.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_005196288.3	XP_005196345.1	ral GTPase-activating protein subunit alpha-1 isoform X6
XM_005196292.3	XP_005196349.1	ral GTPase-activating protein subunit alpha-1 isoform X8
XM_010817181.3	XP_010815483.1	ral GTPase-activating protein subunit alpha-1 isoform X3
XM_010817182.3	XP_010815484.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_010817183.3	XP_010815485.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_010817179.2	XP_010815481.1	ral GTPase-activating protein subunit alpha-1 isoform X1
XM_005222076.3	XP_005222133.1	ral GTPase-activating protein subunit alpha-1 isoform X6
XM_003587576.4	XP_003587624.1	ral GTPase-activating protein subunit alpha-1 isoform X7
XM_003587576.5	XP_003587624.1	ral GTPase-activating protein subunit alpha-1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa94259	XM_015459295.2 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa94259	XM_015468546.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa10828	XM_005222076.4 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa10828	XM_005196288.3 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa10830	XM_003583756.4 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa10830	XM_003587576.5 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa10832	XM_005222080.4 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa10832	XM_005196292.3 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa56727	XM_010817179.3 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56727	XM_010824902.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56728	XM_010817180.3 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56728	XM_010824903.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56729	XM_010824904.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56729	XM_010817181.3 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56730	XM_010824906.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56730	XM_010817182.3 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56730	XM_010824905.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56730	XM_010817183.3 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
OBa10832	XM_005222080.3	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa56728	XM_010817180.2	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa94259	XM_015459295.1	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa56729	XM_010817181.2	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56730	XM_010817182.2	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56730	XM_010817183.2	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa56727	XM_010817179.2	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa10828	XM_005222076.3	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa10830	XM_003587576.4	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

RALGAPA1 ( XM_015459295.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314781.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa94259
Clone ID Related Accession (Same CDS sequence)	XM_015459295.2 , XM_015468546.1 , XM_015459295.1
Accession Version	XM_015459295.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459295.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAGAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCATGCAT TGACAATGTC 
ACAGAGCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGACACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GCACCTACCA AGATCTTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAACTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAAT CTGTACTGAA GATGCCATCA CAAGCTTTTC TGCAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGGGAACTTT GGGATGACTT ACTCTCGGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTGAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTATAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAAAAACA AAAAAAGCAC AAAGGGAAAG GAGTTGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTGC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAACGAG TTAGATAAAG ATGCAGCTTC ACAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAGTA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA CATACAGTTT 
CAAAAAGAAC CAAACTCCGC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTATGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATATA ACACTAGCTG AAAAGGTTAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCGTCAG CAAGCAGTGA AACAATTAAG 
GAAACAACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCCCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAGCGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT ATTCTAAATA AGCAAAACCA GATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAATATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCGGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATCGACTCGG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGATGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTAAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAAAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTATGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AATATAATGC ATTGTGGATT ACTTCATATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CCACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAATTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCATATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACAG AAGCATCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTCCTTA ACTGTATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCCTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAAA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT TACACATCTG GTGAATCACT TGGGCCACTA TCCAATGAGT 
GGCGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCGGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTATCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATAAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGGAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTCAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACCCCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CTTGGGACAA AAGGAGGAGT 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA AATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAT TGACCACACC ATATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCACGTG 
TCAACAAGAA TGCCTTCGGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTA ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCGC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG GTATCTGCAA 
ACGATTGTCC AGCACCATTT AGAACCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
CCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT 
AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314781.1
CDS	165..6653
Translation	MRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015459295.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459295.2

ORF Insert Sequence:

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAGAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCATGCAT TGACAATGTC 
ACAGAGCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGACACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GCACCTACCA AGATCTTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAACTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAAT CTGTACTGAA GATGCCATCA CAAGCTTTTC TGCAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGGGAACTTT GGGATGACTT ACTCTCGGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTGAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTATAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAAAAACA AAAAAAGCAC AAAGGGAAAG GAGTTGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTGC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAACGAG TTAGATAAAG ATGCAGCTTC ACAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAGTA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA CATACAGTTT 
CAAAAAGAAC CAAACTCCGC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTATGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATATA ACACTAGCTG AAAAGGTTAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCGTCAG CAAGCAGTGA AACAATTAAG 
GAAACAACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCCCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAGCGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT ATTCTAAATA AGCAAAACCA GATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAATATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCGGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATCGACTCGG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGATGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTAAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAAAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTATGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AATATAATGC ATTGTGGATT ACTTCATATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CCACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAATTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCATATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACAG AAGCATCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTCCTTA ACTGTATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCCTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAAA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT TACACATCTG GTGAATCACT TGGGCCACTA TCCAATGAGT 
GGCGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCGGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTATCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATAAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGGAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTCAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACCCCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CTTGGGACAA AAGGAGGAGT 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA AATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAT TGACCACACC ATATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCACGTG 
TCAACAAGAA TGCCTTCGGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTA ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCGC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG GTATCTGCAA 
ACGATTGTCC AGCACCATTT AGAACCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
CCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT 
AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015468546.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324032.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.

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CloneID	OBa94259
Clone ID Related Accession (Same CDS sequence)	XM_015459295.2 , XM_015468546.1 , XM_015459295.1
Accession Version	XM_015468546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAGAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCATGCAT TGACAATGTC 
ACAGAGCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGACACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GCACCTACCA AGATCTTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAACTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAAT CTGTACTGAA GATGCCATCA CAAGCTTTTC TGCAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGGGAACTTT GGGATGACTT ACTCTCGGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTGAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTATAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAAAAACA AAAAAAGCAC AAAGGGAAAG GAGTTGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTGC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAACGAG TTAGATAAAG ATGCAGCTTC ACAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAGTA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA CATACAGTTT 
CAAAAAGAAC CAAACTCCGC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTATGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATATA ACACTAGCTG AAAAGGTTAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCGTCAG CAAGCAGTGA AACAATTAAG 
GAAACAACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCCCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAGCGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT ATTCTAAATA AGCAAAACCA GATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAATATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCGGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATCGACTCGG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGATGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTAAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAAAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTATGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AATATAATGC ATTGTGGATT ACTTCATATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CCACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAATTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCATATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACAG AAGCATCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTCCTTA ACTGTATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCCTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAAA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT TACACATCTG GTGAATCACT TGGGCCACTA TCCAATGAGT 
GGCGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCGGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTATCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATAAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGGAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTCAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACCCCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CTTGGGACAA AAGGAGGAGT 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA AATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAT TGACCACACC ATATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCACGTG 
TCAACAAGAA TGCCTTCGGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTA ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCGC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG GTATCTGCAA 
ACGATTGTCC AGCACCATTT AGAACCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
CCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT 
AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324032.1
CDS	90..6578
Translation	MRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015468546.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468546.1

ORF Insert Sequence:

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAGAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCATGCAT TGACAATGTC 
ACAGAGCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGACACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GCACCTACCA AGATCTTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAACTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAAT CTGTACTGAA GATGCCATCA CAAGCTTTTC TGCAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGGGAACTTT GGGATGACTT ACTCTCGGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTGAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTATAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAAAAACA AAAAAAGCAC AAAGGGAAAG GAGTTGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTGC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAACGAG TTAGATAAAG ATGCAGCTTC ACAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAGTA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA CATACAGTTT 
CAAAAAGAAC CAAACTCCGC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTATGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATATA ACACTAGCTG AAAAGGTTAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCGTCAG CAAGCAGTGA AACAATTAAG 
GAAACAACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCCCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAGCGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT ATTCTAAATA AGCAAAACCA GATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAATATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCGGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATCGACTCGG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGATGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTAAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAAAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTATGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AATATAATGC ATTGTGGATT ACTTCATATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CCACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAATTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCATATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACAG AAGCATCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTCCTTA ACTGTATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCCTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAAA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT TACACATCTG GTGAATCACT TGGGCCACTA TCCAATGAGT 
GGCGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCGGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTATCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATAAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGGAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTCAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACCCCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CTTGGGACAA AAGGAGGAGT 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA AATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAT TGACCACACC ATATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCACGTG 
TCAACAAGAA TGCCTTCGGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTA ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCGC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG GTATCTGCAA 
ACGATTGTCC AGCACCATTT AGAACCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
CCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT 
AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015459295.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314781.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.

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CloneID	OBa94259
Clone ID Related Accession (Same CDS sequence)	XM_015459295.2 , XM_015468546.1 , XM_015459295.1
Accession Version	XM_015459295.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAGAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCATGCAT TGACAATGTC 
ACAGAGCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGACACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GCACCTACCA AGATCTTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAACTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAAT CTGTACTGAA GATGCCATCA CAAGCTTTTC TGCAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGGGAACTTT GGGATGACTT ACTCTCGGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTGAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTATAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAAAAACA AAAAAAGCAC AAAGGGAAAG GAGTTGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTGC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAACGAG TTAGATAAAG ATGCAGCTTC ACAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAGTA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA CATACAGTTT 
CAAAAAGAAC CAAACTCCGC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTATGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATATA ACACTAGCTG AAAAGGTTAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCGTCAG CAAGCAGTGA AACAATTAAG 
GAAACAACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCCCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAGCGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT ATTCTAAATA AGCAAAACCA GATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAATATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCGGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATCGACTCGG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGATGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTAAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAAAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTATGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AATATAATGC ATTGTGGATT ACTTCATATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CCACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAATTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCATATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACAG AAGCATCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTCCTTA ACTGTATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCCTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAAA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT TACACATCTG GTGAATCACT TGGGCCACTA TCCAATGAGT 
GGCGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCGGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTATCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATAAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGGAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTCAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACCCCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CTTGGGACAA AAGGAGGAGT 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA AATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAT TGACCACACC ATATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCACGTG 
TCAACAAGAA TGCCTTCGGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTA ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCGC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG GTATCTGCAA 
ACGATTGTCC AGCACCATTT AGAACCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
CCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT 
AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314781.1
CDS	90..6578
Translation	MRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015459295.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459295.1

ORF Insert Sequence:

ATGAGAAAAG TGGTAAAAGT ATATCAAGAA TGGATCCAGC AAGAGGAGAA ACCTTTGTTC 
ATGCAAGAGC CTGAAGAAAT TGTGATCTCT TCTTCAGACC TTCCATGCAT TGACAATGTC 
ACAGAGCATG ATATTTCAAT GGAAGAAGGA GAAAAAAGAG AAGAGGAAAA TGACACCAAT 
GTTGCTGATC ATGTTCGAAA TTCCACTTGG CCTAAAAATG GCACCTACCA AGATCTTCTT 
CATAATGCTT CTGAAGAAGC CATAGAACAA AACATACGAG CTGGTGCCCA GGCAGTTTTG 
CAGGTGTTTA TTATAAACTC TTCAAACATA TTTCTTCTTG AACCTGCAAA TGAGATAAAA 
AGTCTTCTGG ATGAGCACAC AGATATGTGT AAACGAATTC TTAACATTTA TCGGTTCATG 
GTTGTGCAAG TATCAATGGA CAAAAAGACT TGGGAACAGA TGCTGCTTGT GTTGCTCAGA 
GTCACGGAAT CTGTACTGAA GATGCCATCA CAAGCTTTTC TGCAGTTCCA GGGGAAAAAA 
AACATGACTT TGGCAGGTCG ACTTGCAGGA CCACTTTTCC AGACTCTTAT AGTTGCCTGG 
ATTAAAGCAA ATCTAAATGT GTACATCTCT CGGGAACTTT GGGATGACTT ACTCTCGGTA 
TTGTCATCGT TGACCTATTG GGAAGAGTTA GCCACTGAAT GGTCGCTGAC TATGGAGACA 
CTAACTAAAG TTTTAGCTAG AAATTTATAT AGTTTGGATC TCAGTGATTT ACCATTGGAT 
AAGCTGAGTG AACAAAAACA AAAAAAGCAC AAAGGGAAAG GAGTTGGACA TGAATTTCAG 
AAAGTTTCAG TTGACAAGTC ATTTTCTAGA GGATGGAGTC GCGATCAGCC TGGCCAAGCC 
CCAATGAGGC AGAGAAGTGC AACAACCACT GGTTCCCCAG GCACAGAAAA GGCAAGGAGT 
ATAGTACGGC AAAAAACTGT TGCCATGAGA AGCCGATCCA TTGGTGAATG TGCTCTGCCA 
TCGGCCTATA TACGCAGTGC TAAAAGTGCT CCTGTTCTGA TCCATACTTC CAAACCCTTC 
TTGCCTGATA TTGTTCTCAC TCCCCTTTCT GATGAGCTTT CAGATATTGA TGATGCTCAA 
ATACTTCCTC GTCCAACTAG AGTCAGACAT TTTTCGCAAA GTGAAGACAC TGGAAATGAA 
GTTTTTGGTG CTTTGAGTGA GGAGCAGCCA TTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA ACGAGCCAAA GGTGCAGTGC CTGTCATTGA CAGTTCATCC 
CGTCATGCAC CAAGCTTGCA GAGTTCCACA GAGGCTTCTT CAATAACTAG ATCCACTGAG 
AGCCACATCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT 
GACCATCTCT GTCACTTAAT AGATCCAGTA GAACTTGCAG ATTCAGCTTT TGAACAAATC 
CAGTACATTG ACCTTGAAGG AGATGATGAT CTTCTTTCCT CCCTGAAAGA ATATTTTAAG 
GAAACCCAAG AAAGTCACAG TAAAAACGAG TTAGATAAAG ATGCAGCTTC ACAGGAAGTG 
ATCTCTGTTG CAGTAAATAA GGATGAAAGA TCATCCTCAG ATAAATTAGA GTACATAGAC 
CAGGAAAGTA AATCAGAAAA TACCACCTCT TTTGTTGGAA CTCCTGAAAA CATACAGTTT 
CAAAAAGAAC CAAACTCCGC TGTCTTCACA AATATTAGTG CACCCAACCA GTTAGACAGC 
TTTATGAAAA CAAAAACTTC TGAAAAATGT AAACAACTAA ACAATGATAA ACAGTCATCG 
GAACCTAGTT CAGGTAGCCC TTGTGATAAA GAAAAGAGAA AACATCTGTA TAGACAAACA 
GCCACAGAAT TAGATGCCTG TATAGATATA ACACTAGCTG AAAAGGTTAA GGGTGTGCAA 
ATTAATGAAA AAATCACTGT CCCACATGTT TTGCATGCCA AGAAAACTAC ACTAAAAGCA 
CCTGTTAACC GTAGAATGCC TCATGTTACA AGCACTAGCA AGCTTTCTCC AACTAAAAGG 
AGCTTGTCTG AAACAGTAAC TCACAGAGCC AAAATCATGA AAATTGCTAC TAAAAAGCGA 
AATAGTGTTC ATGTTACTTT TAGACCTTCT ACTGAGTCAG TTCAGTTTTA TAATCCTCTG 
GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCAGTGGCTT TAGTGGTAGT 
AATAGCAACA TCAGCAACCC CCACACCAGC TCCAACAGTG CTGCAGAGCT AGTAAAACCT 
GGTGTGTACA GGCCTCTAGA TACAATTAGT GCAGCGTCAG CAAGCAGTGA AACAATTAAG 
GAAACAACAG AGATTCCCAC CGCCATATTA CAGAAAGAAG GAATTGCAAC TAATCAGTTA 
GGTAGTCGTA GTACTATTAA GTCATCAAGT CACGAGGCAG GACTACCGCA GGGCTCCCTG 
GGTGGCGTTT ATAAAACTGT TGTCCATGCT CTTTCGAAGC CGAAGGCAAA TGTTTCCCCA 
CAGAGGCAGA ACAGAATGCC ACCAGAGGCT CCACTGAGGG ATCTGTACAG TCATGTAATG 
GGCTATTTTG GAAGGAAAGC TGCAGTCAAT AAAGAGGACA TGAGCACAAA ACTGCCCCCT 
CTTAATAGTG ATATTGGCAG CAGCAATGCT AATGTTCCTG ATCTGATGGA TGAGTTTATA 
GCAGAGCGAC TTCGAAGTGG TAATGCCTCA ACTATGACAC GAAGAGGAAG TAGTCCAGGT 
AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT ATTCTAAATA AGCAAAACCA GATGCGTCCT 
ATTGATGACC CAGGTGTACC CTCAGAATGG ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT 
CTTATCAGCT CAGATAGTCA TTCGGATTCT TTCAGCGCTT TCCAATATGA TGGCCGAAAA 
TTTGACAATT TTGGTTTTGG AGCGGATACT GGGAATGCAT CCTCTGCTGA CATGGATTCA 
GGTTCTGGCC ATTATCAGAG TGCTGAAGAG CAAGAGGTGG CTAGTCTAAC CACACTACAC 
ATCGACTCGG AAACAAGCAG TCTTAATCAA CAAGCTTTCT GTACTGATGT TGCAACGGTT 
ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT TCAGCTCTGG GATCCAGGTC ACAGACACCT 
TCTCCTTCCA CACTAAATAT AGATCACATG GAACAGAAGG ATCTACAGCT CGATGAAAAG 
CTCCACCACT CTGTTCTTCA GACACCAGAT GACCTAGAAA TCAGTGAATT TCCATCTGAA 
TGCTGTAGTG TGATGGCAGG GGGCACTCTT ACAGGATGGC ATGCTGATGT TGCTACTGTA 
ATGTGGCGAA GGATGCTGGG TATTTTGGGA GATGTCAACG CTATTATGGA TCCTGAAATA 
CATGCTCAAG TTTTTGATTA CCTATGTGAA CTTTGGCAGA ATCTAGCTAA GATTAGAGAT 
AATCTTGGCA TTTCAACTGA TAACCTGACC TCCCCTTCTC CACCGGTTTT AATTCCTCCA 
CTGAGAATTC TTACACCTTG GCTTTTTAAG GCAACCATGT TAACTGATAA ATATAAACAA 
GGTAAATTAC ATGCCTATAA ACTTATTTGT AATACAATGA AAAGAAGACA AGATGTTTCT 
CCAAATAGAG ATTTTCTAAC ACATTTCTAC AATATAATGC ATTGTGGATT ACTTCATATT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCAC CTCAATTTTT CTCACTTGGT 
TTGCCTGGTG CCACAATGCT TATTATGGAT TTTATTGTAG CAGCTGGTAG AGTGGCTTCT 
TCAGCTTTTC TCAATGCACC AAGAGTGGAA GCACAAGTTC TTCTGGGATC TTTGGTTTGC 
TTTCCCAACT TGTATTGTGA ACTGCCTGCT CTCCATCCCA ACATCCCTGA TATTGCTGTG 
TCTCAATTTA CAGATGTTAA GGAACTTATA ATCAAAACTG TGTTAAGCTC TGCAAGAGAT 
GAGCCCTCTG GTCCTGCACG ATGTGTAGCA CTTTGCAGTT TAGGTATTTG GATTTGTGAA 
GAATTAGTCC ATGAGTCTCA TCATCCTCAA ATTAAGGAAG CTCTGAATGT AATTTGTGTT 
TCCTTAAAGT TTACTAATAA AACAGTAGCC CATGTAGCTT GTAACATGCT TCATATGCTG 
GTTCATTATG TACCTAGACT TCAGATTTAC CAGCCTGATT CTCCCTTGAA AATTATTCAA 
ATTCTAATAG CCACTATTAC CCATCTTTTG CCAAGTACAG AAGCATCATC TTATGAAATG 
GACAAGAGGT TGGTGGTATC CTTACTTCTG TGTCTTCTGG ACTGGATCAT GGCCTTACCT 
CTTAAGACAC TGCTCCAACC AGTCCATGCT ACAGGAGCAG AAAATGATAA AATAGAAAAA 
TCTGTCCTTA ACTGTATTTA TAAGGTATTA CATGGGTGTG TTTATGGAGC TCAGTGTTTT 
AGCAATCCGA GGTATTTTCC AATAAGCCTT TCTGATTTGG CATCTGTAGA TTATGATCCT 
TTTATGCATT TGGAAAGCCT AAAAGAGCCT GAACCTTTGC ACTCTCCTGA TTCGGAACGA 
TCCTCTAAAC TCCAGCCAGT AACAGAAGTG AAAACTCAAA TGCAGCAAGG ATTAATCTCC 
ATAGCTGCCC GCACTGTTAT TACACATCTG GTGAATCACT TGGGCCACTA TCCAATGAGT 
GGCGGTCCTG CTATGTTAAC AAGTCAGGTG TGTGAGAATC ACGACAATCA TTACAGTGAA 
AGCACTGAAC TTTCCCCGGA ACTCTTCGAG AGTCCAAATA TTCAGTTTTT TGTGTTAAAC 
AATACAACCT TAGTGTCCTG TATCCAGATC AGAGCTGAAG AGAGTATACC TGGAGGAGGT 
TTATCTGCTG GTCTTGCATC AGCCAATTCA AATGTCAGAA TTATAGTACG TGATCTTTCT 
GGAAAATATT CATGGGATTC TGCCATCCTG TATGGACCAC CTCTTGTAAG TGGTCTATCA 
GAACCTACAT CTCTCGTACT TTCATTGTCT CACCAAGAGA AGCCAGAAGA ACCTTCTGCA 
TCTAATGAAT GCTTAGAAGA TATAACAGTT AAAGATGGGA TTTCCCTCCA GTTTAAAAGA 
AGTTTTAGAG AAACTGTACC AACTTGGGAC ACAATAAGAG ATGAAGAAGA TGTTCTTGAT 
GAGCTTTTGC AGTATTTAGG TGTAACCAGT CCTGAATGCT TACAGAGGAC TGGAGTCTCA 
CTTAATATTC CTGCTCCACA GCCTGTGTGC ATTTCTGAAA AACAGGAGAA TGATGTTATT 
AATGCTATCC TTAAGCAGCA TACAGAGGAA AAAGAATTTG TTGAGAAACA CTTCAATGAC 
TTAAACATGA AAGCTGCAGA ACAAGATGAA CCAACCCCTC AGAAACCTCA GTCAGCATTT 
TATTATTGCA GATTGCTTCT TAGTATATTG GGAATGAATT CTTGGGACAA AAGGAGGAGT 
TTTCATCTCC TGAAGAAAAA TGAAAAACTA CTTAGAGAAC TTAGGAACTT GGACTCAAGA 
CAGTGCCGAG AGACACACAA AATTGCAGTA TTTTATGTTG CTGAAGGACA AGAAGACAAA 
CATTCCATTC TCACCAATAC AGGAGGAAGT CAAGCATATG AAGATTTTGT AGCTGGTCTT 
GGTTGGGAGG TAAATCTTAC AAACCATTGT GGTTTCATGG GAGGACTACA AAAAAATAAA 
AGCACTGGAT TGACCACACC ATATTTTGCT ACCTCTACAG TTGAAGTAAT ATTTCACGTG 
TCAACAAGAA TGCCTTCGGA TTCTGATGAC TCTTTGACCA AAAAATTGAG ACATTTAGGA 
AATGATGAAG TGCACATTGT TTGGTCAGAG CATACTAGAG ACTACAGGAG AGGAATTATT 
CCTACAGAAT TTGGTGATGT CCTTATTGTA ATATACCCGA TGAAAAACCA CATGTTCAGT 
ATCCAGATAA TGAAAAAACC AGAGGTTCCC TTCTTTGGTC CACTCTTTGA TGGTGCTATT 
GTGAATGGAA AGGTCCTACC TATTATGGTT CGAGCAACAG CTATAAATGC AAGCCGTGCT 
CTGAAATCGC TGATTCCATT GTATCAAAAC TTCTATGAGG AGAGAGCACG GTATCTGCAA 
ACGATTGTCC AGCACCATTT AGAACCAACG ACATTTGAAG ATTTTGCAGC ACAGGTTTTT 
TCTCCAGCTC CCTACCATCA TTTACCATCT GATGCCGTGG GCTCCTACCC AGAGATCCTA 
CCCAGTGAAA CTCCCACAGC GGCGCAGGTA GACGGGGCCG ACCTGGCCTC TCCAATGTCT 
CCTCGAACGA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT 
AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_005222076.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222133.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X7, mRNA.

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CloneID	OBa10828
Clone ID Related Accession (Same CDS sequence)	XM_005222076.4 , XM_005196288.3 , XM_005222076.3
Accession Version	XM_005222076.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222076.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCCCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAGCGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT 
ATTCTAAATA AGCAAAACCA GATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCGGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATCGACTCGG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGATGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTAAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAAAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTATGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AATATAATGC ATTGTGGATT ACTTCATATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAATTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCATATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACAG AAGCATCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTCCTTA ACTGTATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCCTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAAA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT TACACATCTG 
GTGAATCACT TGGGCCACTA TCCAATGAGT GGCGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCGGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTATCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATAAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGGAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTCAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACCCCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CTTGGGACAA AAGGAGGAGT TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA AATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAT TGACCACACC ATATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCACGTG TCAACAAGAA TGCCTTCGGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTA 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCGC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG GTATCTGCAA ACGATTGTCC AGCACCATTT AGAACCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA CCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222133.1
CDS	954..7352
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_005222076.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222076.4

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCCCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAGCGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT 
ATTCTAAATA AGCAAAACCA GATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCGGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATCGACTCGG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGATGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTAAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAAAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTATGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AATATAATGC ATTGTGGATT ACTTCATATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAATTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCATATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACAG AAGCATCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTCCTTA ACTGTATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCCTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAAA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT TACACATCTG 
GTGAATCACT TGGGCCACTA TCCAATGAGT GGCGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCGGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTATCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATAAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGGAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTCAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACCCCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CTTGGGACAA AAGGAGGAGT TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA AATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAT TGACCACACC ATATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCACGTG TCAACAAGAA TGCCTTCGGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTA 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCGC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG GTATCTGCAA ACGATTGTCC AGCACCATTT AGAACCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA CCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_005196288.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196345.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.

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CloneID	OBa10828
Clone ID Related Accession (Same CDS sequence)	XM_005222076.4 , XM_005196288.3 , XM_005222076.3
Accession Version	XM_005196288.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196288.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCCCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAGCGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT 
ATTCTAAATA AGCAAAACCA GATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCGGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATCGACTCGG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGATGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTAAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAAAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTATGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AATATAATGC ATTGTGGATT ACTTCATATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAATTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCATATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACAG AAGCATCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTCCTTA ACTGTATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCCTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAAA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT TACACATCTG 
GTGAATCACT TGGGCCACTA TCCAATGAGT GGCGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCGGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTATCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATAAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGGAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTCAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACCCCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CTTGGGACAA AAGGAGGAGT TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA AATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAT TGACCACACC ATATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCACGTG TCAACAAGAA TGCCTTCGGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTA 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCGC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG GTATCTGCAA ACGATTGTCC AGCACCATTT AGAACCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA CCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196345.1
CDS	1227..7625
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_005196288.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196288.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCCCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAGCGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT 
ATTCTAAATA AGCAAAACCA GATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCGGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATCGACTCGG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGATGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTAAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAAAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTATGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AATATAATGC ATTGTGGATT ACTTCATATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAATTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCATATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACAG AAGCATCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTCCTTA ACTGTATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCCTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAAA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT TACACATCTG 
GTGAATCACT TGGGCCACTA TCCAATGAGT GGCGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCGGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTATCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATAAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGGAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTCAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACCCCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CTTGGGACAA AAGGAGGAGT TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA AATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAT TGACCACACC ATATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCACGTG TCAACAAGAA TGCCTTCGGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTA 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCGC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG GTATCTGCAA ACGATTGTCC AGCACCATTT AGAACCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA CCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_005222076.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222133.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.

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CloneID	OBa10828
Clone ID Related Accession (Same CDS sequence)	XM_005222076.4 , XM_005196288.3 , XM_005222076.3
Accession Version	XM_005222076.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCCCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAGCGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT 
ATTCTAAATA AGCAAAACCA GATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCGGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATCGACTCGG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGATGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTAAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAAAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTATGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AATATAATGC ATTGTGGATT ACTTCATATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAATTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCATATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACAG AAGCATCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTCCTTA ACTGTATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCCTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAAA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT TACACATCTG 
GTGAATCACT TGGGCCACTA TCCAATGAGT GGCGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCGGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTATCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATAAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGGAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTCAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACCCCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CTTGGGACAA AAGGAGGAGT TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA AATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAT TGACCACACC ATATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCACGTG TCAACAAGAA TGCCTTCGGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTA 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCGC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG GTATCTGCAA ACGATTGTCC AGCACCATTT AGAACCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA CCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222133.1
CDS	1227..7625
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_005222076.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222076.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT 
AAAGAGGACA TGAGCACAAA ACTGCCCCCT CTTAATAGTG ATATTGGCAG CAGCAATGCT 
AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAGCGAC TTCGAAGTGG TAATGCCTCA 
ACTATGACAC GAAGAGGAAG TAGTCCAGGT AGCCTCGAAA TTCCCAAAGA CCTTCCTGAT 
ATTCTAAATA AGCAAAACCA GATGCGTCCT ATTGATGACC CAGGTGTACC CTCAGAATGG 
ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT 
TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGTTTTGG AGCGGATACT 
GGGAATGCAT CCTCTGCTGA CATGGATTCA GGTTCTGGCC ATTATCAGAG TGCTGAAGAG 
CAAGAGGTGG CTAGTCTAAC CACACTACAC ATCGACTCGG AAACAAGCAG TCTTAATCAA 
CAAGCTTTCT GTACTGATGT TGCAACGGTT ACTGGTTCAG AAAGTGCCTC TCCAGTCCAT 
TCAGCTCTGG GATCCAGGTC ACAGACACCT TCTCCTTCCA CACTAAATAT AGATCACATG 
GAACAGAAGG ATCTACAGCT CGATGAAAAG CTCCACCACT CTGTTCTTCA GACACCAGAT 
GACCTAGAAA TCAGTGAATT TCCATCTGAA TGCTGTAGTG TGATGGCAGG GGGCACTCTT 
ACAGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GGATGCTGGG TATTTTGGGA 
GATGTCAACG CTATTATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTATGTGAA 
CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AATCTTGGCA TTTCAACTGA TAACCTGACC 
TCCCCTTCTC CACCGGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG 
GCAACCATGT TAACTGATAA ATATAAACAA GGTAAATTAC ATGCCTATAA ACTTATTTGT 
AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC 
AATATAATGC ATTGTGGATT ACTTCATATT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCAC CTCAATTTTT CTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT 
TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTGGAA 
GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TGTATTGTGA ACTGCCTGCT 
CTCCATCCCA ACATCCCTGA TATTGCTGTG TCTCAATTTA CAGATGTTAA GGAACTTATA 
ATCAAAACTG TGTTAAGCTC TGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA 
CTTTGCAGTT TAGGTATTTG GATTTGTGAA GAATTAGTCC ATGAGTCTCA TCATCCTCAA 
ATTAAGGAAG CTCTGAATGT AATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC 
CATGTAGCTT GTAACATGCT TCATATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC 
CAGCCTGATT CTCCCTTGAA AATTATTCAA ATTCTAATAG CCACTATTAC CCATCTTTTG 
CCAAGTACAG AAGCATCATC TTATGAAATG GACAAGAGGT TGGTGGTATC CTTACTTCTG 
TGTCTTCTGG ACTGGATCAT GGCCTTACCT CTTAAGACAC TGCTCCAACC AGTCCATGCT 
ACAGGAGCAG AAAATGATAA AATAGAAAAA TCTGTCCTTA ACTGTATTTA TAAGGTATTA 
CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCGA GGTATTTTCC AATAAGCCTT 
TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGCCT AAAAGAGCCT 
GAACCTTTGC ACTCTCCTGA TTCGGAACGA TCCTCTAAAC TCCAGCCAGT AACAGAAGTG 
AAAACTCAAA TGCAGCAAGG ATTAATCTCC ATAGCTGCCC GCACTGTTAT TACACATCTG 
GTGAATCACT TGGGCCACTA TCCAATGAGT GGCGGTCCTG CTATGTTAAC AAGTCAGGTG 
TGTGAGAATC ACGACAATCA TTACAGTGAA AGCACTGAAC TTTCCCCGGA ACTCTTCGAG 
AGTCCAAATA TTCAGTTTTT TGTGTTAAAC AATACAACCT TAGTGTCCTG TATCCAGATC 
AGAGCTGAAG AGAGTATACC TGGAGGAGGT TTATCTGCTG GTCTTGCATC AGCCAATTCA 
AATGTCAGAA TTATAGTACG TGATCTTTCT GGAAAATATT CATGGGATTC TGCCATCCTG 
TATGGACCAC CTCTTGTAAG TGGTCTATCA GAACCTACAT CTCTCGTACT TTCATTGTCT 
CACCAAGAGA AGCCAGAAGA ACCTTCTGCA TCTAATGAAT GCTTAGAAGA TATAACAGTT 
AAAGATGGGA TTTCCCTCCA GTTTAAAAGA AGTTTTAGAG AAACTGTACC AACTTGGGAC 
ACAATAAGAG ATGAAGAAGA TGTTCTTGAT GAGCTTTTGC AGTATTTAGG TGTAACCAGT 
CCTGAATGCT TACAGAGGAC TGGAGTCTCA CTTAATATTC CTGCTCCACA GCCTGTGTGC 
ATTTCTGAAA AACAGGAGAA TGATGTTATT AATGCTATCC TTAAGCAGCA TACAGAGGAA 
AAAGAATTTG TTGAGAAACA CTTCAATGAC TTAAACATGA AAGCTGCAGA ACAAGATGAA 
CCAACCCCTC AGAAACCTCA GTCAGCATTT TATTATTGCA GATTGCTTCT TAGTATATTG 
GGAATGAATT CTTGGGACAA AAGGAGGAGT TTTCATCTCC TGAAGAAAAA TGAAAAACTA 
CTTAGAGAAC TTAGGAACTT GGACTCAAGA CAGTGCCGAG AGACACACAA AATTGCAGTA 
TTTTATGTTG CTGAAGGACA AGAAGACAAA CATTCCATTC TCACCAATAC AGGAGGAAGT 
CAAGCATATG AAGATTTTGT AGCTGGTCTT GGTTGGGAGG TAAATCTTAC AAACCATTGT 
GGTTTCATGG GAGGACTACA AAAAAATAAA AGCACTGGAT TGACCACACC ATATTTTGCT 
ACCTCTACAG TTGAAGTAAT ATTTCACGTG TCAACAAGAA TGCCTTCGGA TTCTGATGAC 
TCTTTGACCA AAAAATTGAG ACATTTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAG 
CATACTAGAG ACTACAGGAG AGGAATTATT CCTACAGAAT TTGGTGATGT CCTTATTGTA 
ATATACCCGA TGAAAAACCA CATGTTCAGT ATCCAGATAA TGAAAAAACC AGAGGTTCCC 
TTCTTTGGTC CACTCTTTGA TGGTGCTATT GTGAATGGAA AGGTCCTACC TATTATGGTT 
CGAGCAACAG CTATAAATGC AAGCCGTGCT CTGAAATCGC TGATTCCATT GTATCAAAAC 
TTCTATGAGG AGAGAGCACG GTATCTGCAA ACGATTGTCC AGCACCATTT AGAACCAACG 
ACATTTGAAG ATTTTGCAGC ACAGGTTTTT TCTCCAGCTC CCTACCATCA TTTACCATCT 
GATGCCGTGG GCTCCTACCC AGAGATCCTA CCCAGTGAAA CTCCCACAGC GGCGCAGGTA 
GACGGGGCCG ACCTGGCCTC TCCAATGTCT CCTCGAACGA GCAAAAGCCG CATGTCCATG 
AAGCTGCGTC GCTCCTCTGG CTCCGCCAAT AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_003583756.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003583804.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.

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CloneID	OBa10830
Clone ID Related Accession (Same CDS sequence)	XM_003583756.4 , XM_003587576.4 , XM_003587576.5
Accession Version	XM_003583756.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583756.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAC CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003583804.1
CDS	1224..7481
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_003583756.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003583756.4

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAC CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_003587576.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587624.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.

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CloneID	OBa10830
Clone ID Related Accession (Same CDS sequence)	XM_003583756.4 , XM_003587576.4 , XM_003587576.5
Accession Version	XM_003587576.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587576.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAC CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587624.1
CDS	1224..7481
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_003587576.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587576.4

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAC CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_003587576.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587624.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X8, mRNA.

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CloneID	OBa10830
Clone ID Related Accession (Same CDS sequence)	XM_003583756.4 , XM_003587576.4 , XM_003587576.5
Accession Version	XM_003587576.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587576.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAC CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587624.1
CDS	954..7211
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_003587576.5
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587576.5

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGTGGG CTCCTACCCA 
GAGATCCTAC CCAGTGAAAC TCCCACAGCG GCGCAGGTAG ACGGGGCCGA CCTGGCCTCT 
CCAATGTCTC CTCGAACGAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG CTCCTCTGGC 
TCCGCCAATA AATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_005222080.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222137.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.

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CloneID	OBa10832
Clone ID Related Accession (Same CDS sequence)	XM_005222080.3 , XM_005222080.4 , XM_005196292.3
Accession Version	XM_005222080.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTACCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222137.1
CDS	1224..7478
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_005222080.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222080.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTACCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_005222080.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222137.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X9, mRNA.

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CloneID	OBa10832
Clone ID Related Accession (Same CDS sequence)	XM_005222080.3 , XM_005222080.4 , XM_005196292.3
Accession Version	XM_005222080.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222080.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTACCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222137.1
CDS	954..7208
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_005222080.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222080.4

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTACCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_005196292.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196349.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.

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CloneID	OBa10832
Clone ID Related Accession (Same CDS sequence)	XM_005222080.3 , XM_005222080.4 , XM_005196292.3
Accession Version	XM_005196292.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196292.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTACCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196349.1
CDS	1224..7478
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_005196292.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196292.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCACAAAA 
CTGCCCCCTC TTAATAGTGA TATTGGCAGC AGCAATGCTA ATGTTCCTGA TCTGATGGAT 
GAGTTTATAG CAGAGCGACT TCGAAGTGGT AATGCCTCAA CTATGACACG AAGAGGAAGT 
AGTCCAGGTA GCCTCGAAAT TCCCAAAGAC CTTCCTGATA TTCTAAATAA GCAAAACCAG 
ATGCGTCCTA TTGATGACCC AGGTGTACCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG 
AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT 
GGCCGAAAAT TTGACAATTT TGGTTTTGGA GCGGATACTG GGAATGCATC CTCTGCTGAC 
ATGGATTCAG GTTCTGGCCA TTATCAGAGT GCTGAAGAGC AAGAGGTGGC TAGTCTAACC 
ACACTACACA TCGACTCGGA AACAAGCAGT CTTAATCAAC AAGCTTTCTG TACTGATGTT 
GCAACGGTTA CTGGTTCAGA AAGTGCCTCT CCAGTCCATT CAGCTCTGGG ATCCAGGTCA 
CAGACACCTT CTCCTTCCAC ACTAAATATA GATCACATGG AACAGAAGGA TCTACAGCTC 
GATGAAAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ACCTAGAAAT CAGTGAATTT 
CCATCTGAAT GCTGTAGTGT GATGGCAGGG GGCACTCTTA CAGGATGGCA TGCTGATGTT 
GCTACTGTAA TGTGGCGAAG GATGCTGGGT ATTTTGGGAG ATGTCAACGC TATTATGGAT 
CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTATGTGAAC TTTGGCAGAA TCTAGCTAAG 
ATTAGAGATA ATCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCGGTTTTA 
ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT AACTGATAAA 
TATAAACAAG GTAAATTACA TGCCTATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA 
GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA 
CTTCATATTG ATCAGGATAT TGTCAATACA ATCATCAAGC ACTGCTCACC TCAATTTTTC 
TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA 
GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTGGAAG CACAAGTTCT TCTGGGATCT 
TTGGTTTGCT TTCCCAACTT GTATTGTGAA CTGCCTGCTC TCCATCCCAA CATCCCTGAT 
ATTGCTGTGT CTCAATTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT GTTAAGCTCT 
GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGCAGTTT AGGTATTTGG 
ATTTGTGAAG AATTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTA 
ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ATGTAGCTTG TAACATGCTT 
CATATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA 
ATTATTCAAA TTCTAATAGC CACTATTACC CATCTTTTGC CAAGTACAGA AGCATCATCT 
TATGAAATGG ACAAGAGGTT GGTGGTATCC TTACTTCTGT GTCTTCTGGA CTGGATCATG 
GCCTTACCTC TTAAGACACT GCTCCAACCA GTCCATGCTA CAGGAGCAGA AAATGATAAA 
ATAGAAAAAT CTGTCCTTAA CTGTATTTAT AAGGTATTAC ATGGGTGTGT TTATGGAGCT 
CAGTGTTTTA GCAATCCGAG GTATTTTCCA ATAAGCCTTT CTGATTTGGC ATCTGTAGAT 
TATGATCCTT TTATGCATTT GGAAAGCCTA AAAGAGCCTG AACCTTTGCA CTCTCCTGAT 
TCGGAACGAT CCTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCAAGGA 
TTAATCTCCA TAGCTGCCCG CACTGTTATT ACACATCTGG TGAATCACTT GGGCCACTAT 
CCAATGAGTG GCGGTCCTGC TATGTTAACA AGTCAGGTGT GTGAGAATCA CGACAATCAT 
TACAGTGAAA GCACTGAACT TTCCCCGGAA CTCTTCGAGA GTCCAAATAT TCAGTTTTTT 
GTGTTAAACA ATACAACCTT AGTGTCCTGT ATCCAGATCA GAGCTGAAGA GAGTATACCT 
GGAGGAGGTT TATCTGCTGG TCTTGCATCA GCCAATTCAA ATGTCAGAAT TATAGTACGT 
GATCTTTCTG GAAAATATTC ATGGGATTCT GCCATCCTGT ATGGACCACC TCTTGTAAGT 
GGTCTATCAG AACCTACATC TCTCGTACTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAA 
CCTTCTGCAT CTAATGAATG CTTAGAAGAT ATAACAGTTA AAGATGGGAT TTCCCTCCAG 
TTTAAAAGAA GTTTTAGAGA AACTGTACCA ACTTGGGACA CAATAAGAGA TGAAGAAGAT 
GTTCTTGATG AGCTTTTGCA GTATTTAGGT GTAACCAGTC CTGAATGCTT ACAGAGGACT 
GGAGTCTCAC TTAATATTCC TGCTCCACAG CCTGTGTGCA TTTCTGAAAA ACAGGAGAAT 
GATGTTATTA ATGCTATCCT TAAGCAGCAT ACAGAGGAAA AAGAATTTGT TGAGAAACAC 
TTCAATGACT TAAACATGAA AGCTGCAGAA CAAGATGAAC CAACCCCTCA GAAACCTCAG 
TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC TTGGGACAAA 
AGGAGGAGTT TTCATCTCCT GAAGAAAAAT GAAAAACTAC TTAGAGAACT TAGGAACTTG 
GACTCAAGAC AGTGCCGAGA GACACACAAA ATTGCAGTAT TTTATGTTGC TGAAGGACAA 
GAAGACAAAC ATTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA 
GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTCATGGG AGGACTACAA 
AAAAATAAAA GCACTGGATT GACCACACCA TATTTTGCTA CCTCTACAGT TGAAGTAATA 
TTTCACGTGT CAACAAGAAT GCCTTCGGAT TCTGATGACT CTTTGACCAA AAAATTGAGA 
CATTTAGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA 
GGAATTATTC CTACAGAATT TGGTGATGTC CTTATTGTAA TATACCCGAT GAAAAACCAC 
ATGTTCAGTA TCCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC ACTCTTTGAT 
GGTGCTATTG TGAATGGAAA GGTCCTACCT ATTATGGTTC GAGCAACAGC TATAAATGCA 
AGCCGTGCTC TGAAATCGCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGG 
TATCTGCAAA CGATTGTCCA GCACCATTTA GAACCAACGA CATTTGAAGA TTTTGCAGCA 
CAGGTTTTTT CTCCAGCTCC CTACCATCAT TTACCATCTG ATGCCGGCTC CTACCCAGAG 
ATCCTACCCA GTGAAACTCC CACAGCGGCG CAGGTAGACG GGGCCGACCT GGCCTCTCCA 
ATGTCTCCTC GAACGAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGCTC CTCTGGCTCC 
GCCAATAAAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010824902.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823204.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

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CloneID	OBa56727
Clone ID Related Accession (Same CDS sequence)	XM_010824902.2 , XM_010817179.3 , XM_010817179.2
Accession Version	XM_010824902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTACCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAATCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823204.1
CDS	1259..9031
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_010824902.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824902.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTACCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAATCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817179.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815481.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.

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CloneID	OBa56727
Clone ID Related Accession (Same CDS sequence)	XM_010824902.2 , XM_010817179.3 , XM_010817179.2
Accession Version	XM_010817179.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTACCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAATCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815481.1
CDS	954..8726
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_010817179.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817179.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTACCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAATCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817179.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815481.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

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CloneID	OBa56727
Clone ID Related Accession (Same CDS sequence)	XM_010824902.2 , XM_010817179.3 , XM_010817179.2
Accession Version	XM_010817179.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTACCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAATCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815481.1
CDS	1259..9031
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_010817179.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817179.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GTGGGCTCCT ACCCAGAGAT CCTACCCAGT GAAACTCCCA CAGCGGCGCA GGTAGACGGG 
GCCGACCTGG CCTCTCCAAT GTCTCCTCGA ACGAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGCTCCT CTGGCTCCGC CAATAAATCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010824903.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823205.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.

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CloneID	OBa56728
Clone ID Related Accession (Same CDS sequence)	XM_010824903.2 , XM_010817180.3 , XM_010817180.2
Accession Version	XM_010824903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GGCTCCTACC CAGAGATCCT ACCCAGTGAA ACTCCCACAG CGGCGCAGGT AGACGGGGCC 
GACCTGGCCT CTCCAATGTC TCCTCGAACG AGCAAAAGCC GCATGTCCAT GAAGCTGCGT 
CGCTCCTCTG GCTCCGCCAA TAAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823205.1
CDS	1258..9027
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_010824903.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824903.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GGCTCCTACC CAGAGATCCT ACCCAGTGAA ACTCCCACAG CGGCGCAGGT AGACGGGGCC 
GACCTGGCCT CTCCAATGTC TCCTCGAACG AGCAAAAGCC GCATGTCCAT GAAGCTGCGT 
CGCTCCTCTG GCTCCGCCAA TAAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817180.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815482.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.

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CloneID	OBa56728
Clone ID Related Accession (Same CDS sequence)	XM_010824903.2 , XM_010817180.3 , XM_010817180.2
Accession Version	XM_010817180.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817180.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GGCTCCTACC CAGAGATCCT ACCCAGTGAA ACTCCCACAG CGGCGCAGGT AGACGGGGCC 
GACCTGGCCT CTCCAATGTC TCCTCGAACG AGCAAAAGCC GCATGTCCAT GAAGCTGCGT 
CGCTCCTCTG GCTCCGCCAA TAAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815482.1
CDS	954..8723
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_010817180.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817180.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GGCTCCTACC CAGAGATCCT ACCCAGTGAA ACTCCCACAG CGGCGCAGGT AGACGGGGCC 
GACCTGGCCT CTCCAATGTC TCCTCGAACG AGCAAAAGCC GCATGTCCAT GAAGCTGCGT 
CGCTCCTCTG GCTCCGCCAA TAAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817180.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815482.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.

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CloneID	OBa56728
Clone ID Related Accession (Same CDS sequence)	XM_010824903.2 , XM_010817180.3 , XM_010817180.2
Accession Version	XM_010817180.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GGCTCCTACC CAGAGATCCT ACCCAGTGAA ACTCCCACAG CGGCGCAGGT AGACGGGGCC 
GACCTGGCCT CTCCAATGTC TCCTCGAACG AGCAAAAGCC GCATGTCCAT GAAGCTGCGT 
CGCTCCTCTG GCTCCGCCAA TAAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815482.1
CDS	1258..9027
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_010817180.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817180.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GGCTCCTACC CAGAGATCCT ACCCAGTGAA ACTCCCACAG CGGCGCAGGT AGACGGGGCC 
GACCTGGCCT CTCCAATGTC TCCTCGAACG AGCAAAAGCC GCATGTCCAT GAAGCTGCGT 
CGCTCCTCTG GCTCCGCCAA TAAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010824904.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823206.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.

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CloneID	OBa56729
Clone ID Related Accession (Same CDS sequence)	XM_010824904.2 , XM_010817181.2 , XM_010817181.3
Accession Version	XM_010824904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TGCCTGTCAT TGACAGTTCA 
TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT 
GAGAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA 
TATGACCATC TCTGTCACTT AATAGATCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA 
ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCTCCCTGAA AGAATATTTT 
AAGGAAACCC AAGAAAGTCA CAGTAAAAAC GAGTTAGATA AAGATGCAGC TTCACAGGAA 
GTGATCTCTG TTGCAGTAAA TAAGGATGAA AGATCATCCT CAGATAAATT AGAGTACATA 
GACCAGGAAA GTAAATCAGA AAATACCACC TCTTTTGTTG GAACTCCTGA AAACATACAG 
TTTCAAAAAG AACCAAACTC CGCTGTCTTC ACAAATATTA GTGCACCCAA CCAGTTAGAC 
AGCTTTATGA AAACAAAAAC TTCTGAAAAA TGTAAACAAC TAAACAATGA TAAACAGTCA 
TCGGAACCTA GTTCAGGTAG CCCTTGTGAT AAAGAAAAGA GAAAACATCT GTATAGACAA 
ACAGCCACAG AATTAGATGC CTGTATAGAT ATAACACTAG CTGAAAAGGT TAAGGGTGTG 
CAAATTAATG AAAAAATCAC TGTCCCACAT GTTTTGCATG CCAAGAAAAC TACACTAAAA 
GCACCTGTTA ACCGTAGAAT GCCTCATGTT ACAAGCACTA GCAAGCTTTC TCCAACTAAA 
AGGAGCTTGT CTGAAACAGT AACTCACAGA GCCAAAATCA TGAAAATTGC TACTAAAAAG 
CGAAATAGTG TTCATGTTAC TTTTAGACCT TCTACTGAGT CAGTTCAGTT TTATAATCCT 
CTGGAAAACA AAGAGGCTCC ATGGAAGATG AGACTGCGGA AGCTCAGTGG CTTTAGTGGT 
AGTAATAGCA ACATCAGCAA CCCCCACACC AGCTCCAACA GTGCTGCAGA GCTAGTAAAA 
CCTGGTGTGT ACAGGCCTCT AGATACAATT AGTGCAGCGT CAGCAAGCAG TGAAACAATT 
AAGGAAACAA CAGAGATTCC CACCGCCATA TTACAGAAAG AAGGAATTGC AACTAATCAG 
TTAGGTAGTC GTAGTACTAT TAAGTCATCA AGTCACGAGG CAGGACTACC GCAGGGCTCC 
CTGGGTGGCG TTTATAAAAC TGTTGTCCAT GCTCTTTCGA AGCCGAAGGC AAATGTTTCC 
CCACAGAGGC AGAACAGAAT GCCACCAGAG GCTCCACTGA GGGATCTGTA CAGTCATGTA 
ATGGGCTATT TTGGAAGGAA AGCTGCAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCC 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAGC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTTCCT GATATTCTAA ATAAGCAAAA CCAGATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAATA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCGGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATCGACT CGGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA TGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTAAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAA 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTATGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAATATAA TGCATTGTGG ATTACTTCAT 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCCACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAATTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCATATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CAGAAGCATC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTCC TTAACTGTAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCCTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AAATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATTACACAT CTGGTGAATC ACTTGGGCCA CTATCCAATG 
AGTGGCGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC GGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTA 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATAA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG GACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTCAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACCC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCTTGGGA CAAAAGGAGG 
AGTTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAAATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GATTGACCAC ACCATATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAC 
GTGTCAACAA GAATGCCTTC GGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTAATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CGCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTATCTG 
CAAACGATTG TCCAGCACCA TTTAGAACCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTACCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGCTCCTC TGGCTCCGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823206.1
CDS	1254..8885
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_010824904.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824904.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TGCCTGTCAT TGACAGTTCA 
TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT 
GAGAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA 
TATGACCATC TCTGTCACTT AATAGATCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA 
ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCTCCCTGAA AGAATATTTT 
AAGGAAACCC AAGAAAGTCA CAGTAAAAAC GAGTTAGATA AAGATGCAGC TTCACAGGAA 
GTGATCTCTG TTGCAGTAAA TAAGGATGAA AGATCATCCT CAGATAAATT AGAGTACATA 
GACCAGGAAA GTAAATCAGA AAATACCACC TCTTTTGTTG GAACTCCTGA AAACATACAG 
TTTCAAAAAG AACCAAACTC CGCTGTCTTC ACAAATATTA GTGCACCCAA CCAGTTAGAC 
AGCTTTATGA AAACAAAAAC TTCTGAAAAA TGTAAACAAC TAAACAATGA TAAACAGTCA 
TCGGAACCTA GTTCAGGTAG CCCTTGTGAT AAAGAAAAGA GAAAACATCT GTATAGACAA 
ACAGCCACAG AATTAGATGC CTGTATAGAT ATAACACTAG CTGAAAAGGT TAAGGGTGTG 
CAAATTAATG AAAAAATCAC TGTCCCACAT GTTTTGCATG CCAAGAAAAC TACACTAAAA 
GCACCTGTTA ACCGTAGAAT GCCTCATGTT ACAAGCACTA GCAAGCTTTC TCCAACTAAA 
AGGAGCTTGT CTGAAACAGT AACTCACAGA GCCAAAATCA TGAAAATTGC TACTAAAAAG 
CGAAATAGTG TTCATGTTAC TTTTAGACCT TCTACTGAGT CAGTTCAGTT TTATAATCCT 
CTGGAAAACA AAGAGGCTCC ATGGAAGATG AGACTGCGGA AGCTCAGTGG CTTTAGTGGT 
AGTAATAGCA ACATCAGCAA CCCCCACACC AGCTCCAACA GTGCTGCAGA GCTAGTAAAA 
CCTGGTGTGT ACAGGCCTCT AGATACAATT AGTGCAGCGT CAGCAAGCAG TGAAACAATT 
AAGGAAACAA CAGAGATTCC CACCGCCATA TTACAGAAAG AAGGAATTGC AACTAATCAG 
TTAGGTAGTC GTAGTACTAT TAAGTCATCA AGTCACGAGG CAGGACTACC GCAGGGCTCC 
CTGGGTGGCG TTTATAAAAC TGTTGTCCAT GCTCTTTCGA AGCCGAAGGC AAATGTTTCC 
CCACAGAGGC AGAACAGAAT GCCACCAGAG GCTCCACTGA GGGATCTGTA CAGTCATGTA 
ATGGGCTATT TTGGAAGGAA AGCTGCAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCC 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAGC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTTCCT GATATTCTAA ATAAGCAAAA CCAGATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAATA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCGGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATCGACT CGGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA TGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTAAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAA 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTATGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAATATAA TGCATTGTGG ATTACTTCAT 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCCACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAATTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCATATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CAGAAGCATC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTCC TTAACTGTAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCCTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AAATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATTACACAT CTGGTGAATC ACTTGGGCCA CTATCCAATG 
AGTGGCGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC GGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTA 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATAA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG GACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTCAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACCC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCTTGGGA CAAAAGGAGG 
AGTTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAAATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GATTGACCAC ACCATATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAC 
GTGTCAACAA GAATGCCTTC GGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTAATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CGCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTATCTG 
CAAACGATTG TCCAGCACCA TTTAGAACCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTACCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGCTCCTC TGGCTCCGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817181.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815483.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.

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CloneID	OBa56729
Clone ID Related Accession (Same CDS sequence)	XM_010824904.2 , XM_010817181.2 , XM_010817181.3
Accession Version	XM_010817181.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TGCCTGTCAT TGACAGTTCA 
TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT 
GAGAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA 
TATGACCATC TCTGTCACTT AATAGATCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA 
ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCTCCCTGAA AGAATATTTT 
AAGGAAACCC AAGAAAGTCA CAGTAAAAAC GAGTTAGATA AAGATGCAGC TTCACAGGAA 
GTGATCTCTG TTGCAGTAAA TAAGGATGAA AGATCATCCT CAGATAAATT AGAGTACATA 
GACCAGGAAA GTAAATCAGA AAATACCACC TCTTTTGTTG GAACTCCTGA AAACATACAG 
TTTCAAAAAG AACCAAACTC CGCTGTCTTC ACAAATATTA GTGCACCCAA CCAGTTAGAC 
AGCTTTATGA AAACAAAAAC TTCTGAAAAA TGTAAACAAC TAAACAATGA TAAACAGTCA 
TCGGAACCTA GTTCAGGTAG CCCTTGTGAT AAAGAAAAGA GAAAACATCT GTATAGACAA 
ACAGCCACAG AATTAGATGC CTGTATAGAT ATAACACTAG CTGAAAAGGT TAAGGGTGTG 
CAAATTAATG AAAAAATCAC TGTCCCACAT GTTTTGCATG CCAAGAAAAC TACACTAAAA 
GCACCTGTTA ACCGTAGAAT GCCTCATGTT ACAAGCACTA GCAAGCTTTC TCCAACTAAA 
AGGAGCTTGT CTGAAACAGT AACTCACAGA GCCAAAATCA TGAAAATTGC TACTAAAAAG 
CGAAATAGTG TTCATGTTAC TTTTAGACCT TCTACTGAGT CAGTTCAGTT TTATAATCCT 
CTGGAAAACA AAGAGGCTCC ATGGAAGATG AGACTGCGGA AGCTCAGTGG CTTTAGTGGT 
AGTAATAGCA ACATCAGCAA CCCCCACACC AGCTCCAACA GTGCTGCAGA GCTAGTAAAA 
CCTGGTGTGT ACAGGCCTCT AGATACAATT AGTGCAGCGT CAGCAAGCAG TGAAACAATT 
AAGGAAACAA CAGAGATTCC CACCGCCATA TTACAGAAAG AAGGAATTGC AACTAATCAG 
TTAGGTAGTC GTAGTACTAT TAAGTCATCA AGTCACGAGG CAGGACTACC GCAGGGCTCC 
CTGGGTGGCG TTTATAAAAC TGTTGTCCAT GCTCTTTCGA AGCCGAAGGC AAATGTTTCC 
CCACAGAGGC AGAACAGAAT GCCACCAGAG GCTCCACTGA GGGATCTGTA CAGTCATGTA 
ATGGGCTATT TTGGAAGGAA AGCTGCAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCC 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAGC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTTCCT GATATTCTAA ATAAGCAAAA CCAGATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAATA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCGGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATCGACT CGGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA TGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTAAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAA 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTATGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAATATAA TGCATTGTGG ATTACTTCAT 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCCACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAATTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCATATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CAGAAGCATC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTCC TTAACTGTAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCCTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AAATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATTACACAT CTGGTGAATC ACTTGGGCCA CTATCCAATG 
AGTGGCGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC GGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTA 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATAA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG GACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTCAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACCC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCTTGGGA CAAAAGGAGG 
AGTTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAAATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GATTGACCAC ACCATATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAC 
GTGTCAACAA GAATGCCTTC GGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTAATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CGCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTATCTG 
CAAACGATTG TCCAGCACCA TTTAGAACCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTACCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGCTCCTC TGGCTCCGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815483.1
CDS	1254..8885
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_010817181.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817181.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TGCCTGTCAT TGACAGTTCA 
TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT 
GAGAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA 
TATGACCATC TCTGTCACTT AATAGATCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA 
ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCTCCCTGAA AGAATATTTT 
AAGGAAACCC AAGAAAGTCA CAGTAAAAAC GAGTTAGATA AAGATGCAGC TTCACAGGAA 
GTGATCTCTG TTGCAGTAAA TAAGGATGAA AGATCATCCT CAGATAAATT AGAGTACATA 
GACCAGGAAA GTAAATCAGA AAATACCACC TCTTTTGTTG GAACTCCTGA AAACATACAG 
TTTCAAAAAG AACCAAACTC CGCTGTCTTC ACAAATATTA GTGCACCCAA CCAGTTAGAC 
AGCTTTATGA AAACAAAAAC TTCTGAAAAA TGTAAACAAC TAAACAATGA TAAACAGTCA 
TCGGAACCTA GTTCAGGTAG CCCTTGTGAT AAAGAAAAGA GAAAACATCT GTATAGACAA 
ACAGCCACAG AATTAGATGC CTGTATAGAT ATAACACTAG CTGAAAAGGT TAAGGGTGTG 
CAAATTAATG AAAAAATCAC TGTCCCACAT GTTTTGCATG CCAAGAAAAC TACACTAAAA 
GCACCTGTTA ACCGTAGAAT GCCTCATGTT ACAAGCACTA GCAAGCTTTC TCCAACTAAA 
AGGAGCTTGT CTGAAACAGT AACTCACAGA GCCAAAATCA TGAAAATTGC TACTAAAAAG 
CGAAATAGTG TTCATGTTAC TTTTAGACCT TCTACTGAGT CAGTTCAGTT TTATAATCCT 
CTGGAAAACA AAGAGGCTCC ATGGAAGATG AGACTGCGGA AGCTCAGTGG CTTTAGTGGT 
AGTAATAGCA ACATCAGCAA CCCCCACACC AGCTCCAACA GTGCTGCAGA GCTAGTAAAA 
CCTGGTGTGT ACAGGCCTCT AGATACAATT AGTGCAGCGT CAGCAAGCAG TGAAACAATT 
AAGGAAACAA CAGAGATTCC CACCGCCATA TTACAGAAAG AAGGAATTGC AACTAATCAG 
TTAGGTAGTC GTAGTACTAT TAAGTCATCA AGTCACGAGG CAGGACTACC GCAGGGCTCC 
CTGGGTGGCG TTTATAAAAC TGTTGTCCAT GCTCTTTCGA AGCCGAAGGC AAATGTTTCC 
CCACAGAGGC AGAACAGAAT GCCACCAGAG GCTCCACTGA GGGATCTGTA CAGTCATGTA 
ATGGGCTATT TTGGAAGGAA AGCTGCAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCC 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAGC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTTCCT GATATTCTAA ATAAGCAAAA CCAGATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAATA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCGGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATCGACT CGGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA TGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTAAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAA 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTATGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAATATAA TGCATTGTGG ATTACTTCAT 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCCACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAATTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCATATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CAGAAGCATC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTCC TTAACTGTAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCCTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AAATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATTACACAT CTGGTGAATC ACTTGGGCCA CTATCCAATG 
AGTGGCGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC GGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTA 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATAA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG GACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTCAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACCC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCTTGGGA CAAAAGGAGG 
AGTTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAAATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GATTGACCAC ACCATATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAC 
GTGTCAACAA GAATGCCTTC GGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTAATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CGCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTATCTG 
CAAACGATTG TCCAGCACCA TTTAGAACCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTACCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGCTCCTC TGGCTCCGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817181.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815483.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.

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CloneID	OBa56729
Clone ID Related Accession (Same CDS sequence)	XM_010824904.2 , XM_010817181.2 , XM_010817181.3
Accession Version	XM_010817181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TGCCTGTCAT TGACAGTTCA 
TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT 
GAGAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA 
TATGACCATC TCTGTCACTT AATAGATCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA 
ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCTCCCTGAA AGAATATTTT 
AAGGAAACCC AAGAAAGTCA CAGTAAAAAC GAGTTAGATA AAGATGCAGC TTCACAGGAA 
GTGATCTCTG TTGCAGTAAA TAAGGATGAA AGATCATCCT CAGATAAATT AGAGTACATA 
GACCAGGAAA GTAAATCAGA AAATACCACC TCTTTTGTTG GAACTCCTGA AAACATACAG 
TTTCAAAAAG AACCAAACTC CGCTGTCTTC ACAAATATTA GTGCACCCAA CCAGTTAGAC 
AGCTTTATGA AAACAAAAAC TTCTGAAAAA TGTAAACAAC TAAACAATGA TAAACAGTCA 
TCGGAACCTA GTTCAGGTAG CCCTTGTGAT AAAGAAAAGA GAAAACATCT GTATAGACAA 
ACAGCCACAG AATTAGATGC CTGTATAGAT ATAACACTAG CTGAAAAGGT TAAGGGTGTG 
CAAATTAATG AAAAAATCAC TGTCCCACAT GTTTTGCATG CCAAGAAAAC TACACTAAAA 
GCACCTGTTA ACCGTAGAAT GCCTCATGTT ACAAGCACTA GCAAGCTTTC TCCAACTAAA 
AGGAGCTTGT CTGAAACAGT AACTCACAGA GCCAAAATCA TGAAAATTGC TACTAAAAAG 
CGAAATAGTG TTCATGTTAC TTTTAGACCT TCTACTGAGT CAGTTCAGTT TTATAATCCT 
CTGGAAAACA AAGAGGCTCC ATGGAAGATG AGACTGCGGA AGCTCAGTGG CTTTAGTGGT 
AGTAATAGCA ACATCAGCAA CCCCCACACC AGCTCCAACA GTGCTGCAGA GCTAGTAAAA 
CCTGGTGTGT ACAGGCCTCT AGATACAATT AGTGCAGCGT CAGCAAGCAG TGAAACAATT 
AAGGAAACAA CAGAGATTCC CACCGCCATA TTACAGAAAG AAGGAATTGC AACTAATCAG 
TTAGGTAGTC GTAGTACTAT TAAGTCATCA AGTCACGAGG CAGGACTACC GCAGGGCTCC 
CTGGGTGGCG TTTATAAAAC TGTTGTCCAT GCTCTTTCGA AGCCGAAGGC AAATGTTTCC 
CCACAGAGGC AGAACAGAAT GCCACCAGAG GCTCCACTGA GGGATCTGTA CAGTCATGTA 
ATGGGCTATT TTGGAAGGAA AGCTGCAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCC 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAGC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTTCCT GATATTCTAA ATAAGCAAAA CCAGATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAATA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCGGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATCGACT CGGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA TGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTAAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAA 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTATGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAATATAA TGCATTGTGG ATTACTTCAT 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCCACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAATTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCATATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CAGAAGCATC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTCC TTAACTGTAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCCTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AAATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATTACACAT CTGGTGAATC ACTTGGGCCA CTATCCAATG 
AGTGGCGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC GGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTA 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATAA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG GACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTCAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACCC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCTTGGGA CAAAAGGAGG 
AGTTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAAATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GATTGACCAC ACCATATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAC 
GTGTCAACAA GAATGCCTTC GGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTAATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CGCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTATCTG 
CAAACGATTG TCCAGCACCA TTTAGAACCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTACCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGCTCCTC TGGCTCCGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815483.1
CDS	954..8585
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPEILPSETPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_010817181.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817181.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGATAT TGATGATGCT 
CAAATACTTC CTCGTCCAAC TAGAGTCAGA CATTTTTCGC AAAGTGAAGA CACTGGAAAT 
GAAGTTTTTG GTGCTTTGAG TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC 
ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TGCCTGTCAT TGACAGTTCA 
TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT 
GAGAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA 
TATGACCATC TCTGTCACTT AATAGATCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA 
ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCTCCCTGAA AGAATATTTT 
AAGGAAACCC AAGAAAGTCA CAGTAAAAAC GAGTTAGATA AAGATGCAGC TTCACAGGAA 
GTGATCTCTG TTGCAGTAAA TAAGGATGAA AGATCATCCT CAGATAAATT AGAGTACATA 
GACCAGGAAA GTAAATCAGA AAATACCACC TCTTTTGTTG GAACTCCTGA AAACATACAG 
TTTCAAAAAG AACCAAACTC CGCTGTCTTC ACAAATATTA GTGCACCCAA CCAGTTAGAC 
AGCTTTATGA AAACAAAAAC TTCTGAAAAA TGTAAACAAC TAAACAATGA TAAACAGTCA 
TCGGAACCTA GTTCAGGTAG CCCTTGTGAT AAAGAAAAGA GAAAACATCT GTATAGACAA 
ACAGCCACAG AATTAGATGC CTGTATAGAT ATAACACTAG CTGAAAAGGT TAAGGGTGTG 
CAAATTAATG AAAAAATCAC TGTCCCACAT GTTTTGCATG CCAAGAAAAC TACACTAAAA 
GCACCTGTTA ACCGTAGAAT GCCTCATGTT ACAAGCACTA GCAAGCTTTC TCCAACTAAA 
AGGAGCTTGT CTGAAACAGT AACTCACAGA GCCAAAATCA TGAAAATTGC TACTAAAAAG 
CGAAATAGTG TTCATGTTAC TTTTAGACCT TCTACTGAGT CAGTTCAGTT TTATAATCCT 
CTGGAAAACA AAGAGGCTCC ATGGAAGATG AGACTGCGGA AGCTCAGTGG CTTTAGTGGT 
AGTAATAGCA ACATCAGCAA CCCCCACACC AGCTCCAACA GTGCTGCAGA GCTAGTAAAA 
CCTGGTGTGT ACAGGCCTCT AGATACAATT AGTGCAGCGT CAGCAAGCAG TGAAACAATT 
AAGGAAACAA CAGAGATTCC CACCGCCATA TTACAGAAAG AAGGAATTGC AACTAATCAG 
TTAGGTAGTC GTAGTACTAT TAAGTCATCA AGTCACGAGG CAGGACTACC GCAGGGCTCC 
CTGGGTGGCG TTTATAAAAC TGTTGTCCAT GCTCTTTCGA AGCCGAAGGC AAATGTTTCC 
CCACAGAGGC AGAACAGAAT GCCACCAGAG GCTCCACTGA GGGATCTGTA CAGTCATGTA 
ATGGGCTATT TTGGAAGGAA AGCTGCAGTC AATAAAGAGG ACATGAGCAC AAAACTGCCC 
CCTCTTAATA GTGATATTGG CAGCAGCAAT GCTAATGTTC CTGATCTGAT GGATGAGTTT 
ATAGCAGAGC GACTTCGAAG TGGTAATGCC TCAACTATGA CACGAAGAGG AAGTAGTCCA 
GGTAGCCTCG AAATTCCCAA AGACCTTCCT GATATTCTAA ATAAGCAAAA CCAGATGCGT 
CCTATTGATG ACCCAGGTGT ACCCTCAGAA TGGACTTCTC CTGCCAGTGC AGGGAGCAGT 
GATCTTATCA GCTCAGATAG TCATTCGGAT TCTTTCAGCG CTTTCCAATA TGATGGCCGA 
AAATTTGACA ATTTTGGTTT TGGAGCGGAT ACTGGGAATG CATCCTCTGC TGACATGGAT 
TCAGGTTCTG GCCATTATCA GAGTGCTGAA GAGCAAGAGG TGGCTAGTCT AACCACACTA 
CACATCGACT CGGAAACAAG CAGTCTTAAT CAACAAGCTT TCTGTACTGA TGTTGCAACG 
GTTACTGGTT CAGAAAGTGC CTCTCCAGTC CATTCAGCTC TGGGATCCAG GTCACAGACA 
CCTTCTCCTT CCACACTAAA TATAGATCAC ATGGAACAGA AGGATCTACA GCTCGATGAA 
AAGCTCCACC ACTCTGTTCT TCAGACACCA GATGACCTAG AAATCAGTGA ATTTCCATCT 
GAATGCTGTA GTGTGATGGC AGGGGGCACT CTTACAGGAT GGCATGCTGA TGTTGCTACT 
GTAATGTGGC GAAGGATGCT GGGTATTTTG GGAGATGTCA ACGCTATTAT GGATCCTGAA 
ATACATGCTC AAGTTTTTGA TTACCTATGT GAACTTTGGC AGAATCTAGC TAAGATTAGA 
GATAATCTTG GCATTTCAAC TGATAACCTG ACCTCCCCTT CTCCACCGGT TTTAATTCCT 
CCACTGAGAA TTCTTACACC TTGGCTTTTT AAGGCAACCA TGTTAACTGA TAAATATAAA 
CAAGGTAAAT TACATGCCTA TAAACTTATT TGTAATACAA TGAAAAGAAG ACAAGATGTT 
TCTCCAAATA GAGATTTTCT AACACATTTC TACAATATAA TGCATTGTGG ATTACTTCAT 
ATTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CACCTCAATT TTTCTCACTT 
GGTTTGCCTG GTGCCACAAT GCTTATTATG GATTTTATTG TAGCAGCTGG TAGAGTGGCT 
TCTTCAGCTT TTCTCAATGC ACCAAGAGTG GAAGCACAAG TTCTTCTGGG ATCTTTGGTT 
TGCTTTCCCA ACTTGTATTG TGAACTGCCT GCTCTCCATC CCAACATCCC TGATATTGCT 
GTGTCTCAAT TTACAGATGT TAAGGAACTT ATAATCAAAA CTGTGTTAAG CTCTGCAAGA 
GATGAGCCCT CTGGTCCTGC ACGATGTGTA GCACTTTGCA GTTTAGGTAT TTGGATTTGT 
GAAGAATTAG TCCATGAGTC TCATCATCCT CAAATTAAGG AAGCTCTGAA TGTAATTTGT 
GTTTCCTTAA AGTTTACTAA TAAAACAGTA GCCCATGTAG CTTGTAACAT GCTTCATATG 
CTGGTTCATT ATGTACCTAG ACTTCAGATT TACCAGCCTG ATTCTCCCTT GAAAATTATT 
CAAATTCTAA TAGCCACTAT TACCCATCTT TTGCCAAGTA CAGAAGCATC ATCTTATGAA 
ATGGACAAGA GGTTGGTGGT ATCCTTACTT CTGTGTCTTC TGGACTGGAT CATGGCCTTA 
CCTCTTAAGA CACTGCTCCA ACCAGTCCAT GCTACAGGAG CAGAAAATGA TAAAATAGAA 
AAATCTGTCC TTAACTGTAT TTATAAGGTA TTACATGGGT GTGTTTATGG AGCTCAGTGT 
TTTAGCAATC CGAGGTATTT TCCAATAAGC CTTTCTGATT TGGCATCTGT AGATTATGAT 
CCTTTTATGC ATTTGGAAAG CCTAAAAGAG CCTGAACCTT TGCACTCTCC TGATTCGGAA 
CGATCCTCTA AACTCCAGCC AGTAACAGAA GTGAAAACTC AAATGCAGCA AGGATTAATC 
TCCATAGCTG CCCGCACTGT TATTACACAT CTGGTGAATC ACTTGGGCCA CTATCCAATG 
AGTGGCGGTC CTGCTATGTT AACAAGTCAG GTGTGTGAGA ATCACGACAA TCATTACAGT 
GAAAGCACTG AACTTTCCCC GGAACTCTTC GAGAGTCCAA ATATTCAGTT TTTTGTGTTA 
AACAATACAA CCTTAGTGTC CTGTATCCAG ATCAGAGCTG AAGAGAGTAT ACCTGGAGGA 
GGTTTATCTG CTGGTCTTGC ATCAGCCAAT TCAAATGTCA GAATTATAGT ACGTGATCTT 
TCTGGAAAAT ATTCATGGGA TTCTGCCATC CTGTATGGAC CACCTCTTGT AAGTGGTCTA 
TCAGAACCTA CATCTCTCGT ACTTTCATTG TCTCACCAAG AGAAGCCAGA AGAACCTTCT 
GCATCTAATG AATGCTTAGA AGATATAACA GTTAAAGATG GGATTTCCCT CCAGTTTAAA 
AGAAGTTTTA GAGAAACTGT ACCAACTTGG GACACAATAA GAGATGAAGA AGATGTTCTT 
GATGAGCTTT TGCAGTATTT AGGTGTAACC AGTCCTGAAT GCTTACAGAG GACTGGAGTC 
TCACTTAATA TTCCTGCTCC ACAGCCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTT 
ATTAATGCTA TCCTTAAGCA GCATACAGAG GAAAAAGAAT TTGTTGAGAA ACACTTCAAT 
GACTTAAACA TGAAAGCTGC AGAACAAGAT GAACCAACCC CTCAGAAACC TCAGTCAGCA 
TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCTTGGGA CAAAAGGAGG 
AGTTTTCATC TCCTGAAGAA AAATGAAAAA CTACTTAGAG AACTTAGGAA CTTGGACTCA 
AGACAGTGCC GAGAGACACA CAAAATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC 
AAACATTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTCA TGGGAGGACT ACAAAAAAAT 
AAAAGCACTG GATTGACCAC ACCATATTTT GCTACCTCTA CAGTTGAAGT AATATTTCAC 
GTGTCAACAA GAATGCCTTC GGATTCTGAT GACTCTTTGA CCAAAAAATT GAGACATTTA 
GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTTATT GTAATATACC CGATGAAAAA CCACATGTTC 
AGTATCCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTCTT TGATGGTGCT 
ATTGTGAATG GAAAGGTCCT ACCTATTATG GTTCGAGCAA CAGCTATAAA TGCAAGCCGT 
GCTCTGAAAT CGCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTATCTG 
CAAACGATTG TCCAGCACCA TTTAGAACCA ACGACATTTG AAGATTTTGC AGCACAGGTT 
TTTTCTCCAG CTCCCTACCA TCATTTACCA TCTGATGCCG TGGGCTCCTA CCCAGAGATC 
CTACCCAGTG AAACTCCCAC AGCGGCGCAG GTAGACGGGG CCGACCTGGC CTCTCCAATG 
TCTCCTCGAA CGAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGCTCCTC TGGCTCCGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010824906.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823208.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.

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CloneID	OBa56730
Clone ID Related Accession (Same CDS sequence)	XM_010824906.2 , XM_010817183.2 , XM_010817182.2 , XM_010824905.2 , XM_010817182.3 , XM_010817183.3
Accession Version	XM_010824906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823208.1
CDS	1262..8890
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_010824906.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824906.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817183.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815485.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.

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CloneID	OBa56730
Clone ID Related Accession (Same CDS sequence)	XM_010824906.2 , XM_010817183.2 , XM_010817182.2 , XM_010824905.2 , XM_010817182.3 , XM_010817183.3
Accession Version	XM_010817183.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815485.1
CDS	1262..8890
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_010817183.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817183.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817182.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815484.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.

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CloneID	OBa56730
Clone ID Related Accession (Same CDS sequence)	XM_010824906.2 , XM_010817183.2 , XM_010817182.2 , XM_010824905.2 , XM_010817182.3 , XM_010817183.3
Accession Version	XM_010817182.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815484.1
CDS	1262..8890
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_010817182.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817182.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010824905.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823207.1 Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.

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CloneID	OBa56730
Clone ID Related Accession (Same CDS sequence)	XM_010824906.2 , XM_010817183.2 , XM_010817182.2 , XM_010824905.2 , XM_010817182.3 , XM_010817183.3
Accession Version	XM_010824905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823207.1
CDS	1262..8890
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_010824905.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824905.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817182.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815484.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.

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CloneID	OBa56730
Clone ID Related Accession (Same CDS sequence)	XM_010824906.2 , XM_010817183.2 , XM_010817182.2 , XM_010824905.2 , XM_010817182.3 , XM_010817183.3
Accession Version	XM_010817182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815484.1
CDS	954..8582
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_010817182.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817182.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_010817183.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815485.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X5, mRNA.

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CloneID	OBa56730
Clone ID Related Accession (Same CDS sequence)	XM_010824906.2 , XM_010817183.2 , XM_010817182.2 , XM_010824905.2 , XM_010817182.3 , XM_010817183.3
Accession Version	XM_010817183.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817183.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815485.1
CDS	954..8582
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREENKNDNADKTDRSTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVISSSDLPCIDNVTEHDISMEEGEKREEENDTNVADHVRNSTWPKNGTYQDLLHNASEEAIEQNIRAGAQAVLQVFIINSSNIFLLEPANEIKSLLDEHTDMCKRILNIYRFMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDILEPFTVERAKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHLIDPVELADSAFEQIQYIDLEGDDDLLSSLKEYFKETQESHSKNELDKDAASQEVISVAVNKDERSSSDKLEYIDQESKSENTTSFVGTPENIQFQKEPNSAVFTNISAPNQLDSFMKTKTSEKCKQLNNDKQSSEPSSGSPCDKEKRKHLYRQTATELDACIDITLAEKVKGVQINEKITVPHVLHAKKTTLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSGSNSNISNPHTSSNSAAELVKPGVYRPLDTISAASASSETIKETTEIPTAILQKEGIATNQLGSRSTIKSSSHEAGLPQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDMSTKLPPLNSDIGSSNANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGADTGNASSADMDSGSGHYQSAEEQEVASLTTLHIDSETSSLNQQAFCTDVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKIEKSVLNCIYKVLHGCVYGAQCFSNPRYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPLVSGLSEPTSLVLSLSHQEKPEEPSASNECLEDITVKDGISLQFKRSFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAAEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_010817183.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817183.3

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGATACC 
AAGAAGGACG CGCTGACCCG CCTGAAGCAC CTGCGCATCG TCATTGAGAA TGCAGAATCC 
ATTGATCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATATTATGT GTTTTTTGAG 
AATTTTGTTA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGGGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AACTACTACA CACAGGAAAC 
TCCTTGAAGA TTAGGCGGGA AGGTGTTCGG CTTTTCTTAT TATGGTTGCA AGCTCTTCAG 
AATAACTGTA GCAAAGAACA GCTTTGGATG TTTTCATGCT TAATCCCCGG ATTTTCAGCA 
CCACAGTCTG AATATGGACC TCGAACTTTA GATAACCTCA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTCACTAT AGAGGAAATC ACTCCACTTG TCCCCCCACA ATCAGGAGAT 
AAAGGACAAG AAGATCTTAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA TATAGTAATT 
CAGGTAAAAA GTTTAGAGTG GAAGAACAAA GAAAACCAAG AAAGAGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAATA TTATTTGCCT TATATTTTCC CAAACATCTG TAAGGAGAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCACA TTATGTCATG 
ATCAAGAAGG ATGCTGAAAC CAATGAGGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG 
GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCATTCTGGC TGGAGCCAAA GCCGCATACA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCCA AAAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG CTGATAAAAC AGATAGAAGT 
ACAGAACCTG AACAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGTATTGA TGAAGAGCAT CTCACAGACA TTGAAATAGT TCGCCGAGTT 
TTTTCTTCTA AAAGAAGTAA CGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GCGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC 
CAGCAAGAGG AGAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CTCTTCTTCA 
GACCTTCCAT GCATTGACAA TGTCACAGAG CATGATATTT CAATGGAAGA AGGAGAAAAA 
AGAGAAGAGG AAAATGACAC CAATGTTGCT GATCATGTTC GAAATTCCAC TTGGCCTAAA 
AATGGCACCT ACCAAGATCT TCTTCATAAT GCTTCTGAAG AAGCCATAGA ACAAAACATA 
CGAGCTGGTG CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCTTCAAA CATATTTCTT 
CTTGAACCTG CAAATGAGAT AAAAAGTCTT CTGGATGAGC ACACAGATAT GTGTAAACGA 
ATTCTTAACA TTTATCGGTT CATGGTTGTG CAAGTATCAA TGGACAAAAA GACTTGGGAA 
CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT 
TTTCTGCAGT TCCAGGGGAA AAAAAACATG ACTTTGGCAG GTCGACTTGC AGGACCACTT 
TTCCAGACTC TTATAGTTGC CTGGATTAAA GCAAATCTAA ATGTGTACAT CTCTCGGGAA 
CTTTGGGATG ACTTACTCTC GGTATTGTCA TCGTTGACCT ATTGGGAAGA GTTAGCCACT 
GAATGGTCGC TGACTATGGA GACACTAACT AAAGTTTTAG CTAGAAATTT ATATAGTTTG 
GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAAA AACAAAAAAA GCACAAAGGG 
AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG 
AGTCGCGATC AGCCTGGCCA AGCCCCAATG AGGCAGAGAA GTGCAACAAC CACTGGTTCC 
CCAGGCACAG AAAAGGCAAG GAGTATAGTA CGGCAAAAAA CTGTTGCCAT GAGAAGCCGA 
TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT 
CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG 
CTTTCAGATA TTGATGATGC TCAAATACTT CCTCGTCCAA CTAGAGTCAG ACATTTTTCG 
CAAAGTGAAG ACACTGGAAA TGAAGTTTTT GGTGCTTTGA GTGAGGAGCA GCCATTGCCT 
CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA 
GTGCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT 
TCTTCAATAA CTAGATCCAC TGAGAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT 
CTGGAAGTTG GTGATAGCAT ATATGACCAT CTCTGTCACT TAATAGATCC AGTAGAACTT 
GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT 
TCCTCCCTGA AAGAATATTT TAAGGAAACC CAAGAAAGTC ACAGTAAAAA CGAGTTAGAT 
AAAGATGCAG CTTCACAGGA AGTGATCTCT GTTGCAGTAA ATAAGGATGA AAGATCATCC 
TCAGATAAAT TAGAGTACAT AGACCAGGAA AGTAAATCAG AAAATACCAC CTCTTTTGTT 
GGAACTCCTG AAAACATACA GTTTCAAAAA GAACCAAACT CCGCTGTCTT CACAAATATT 
AGTGCACCCA ACCAGTTAGA CAGCTTTATG AAAACAAAAA CTTCTGAAAA ATGTAAACAA 
CTAAACAATG ATAAACAGTC ATCGGAACCT AGTTCAGGTA GCCCTTGTGA TAAAGAAAAG 
AGAAAACATC TGTATAGACA AACAGCCACA GAATTAGATG CCTGTATAGA TATAACACTA 
GCTGAAAAGG TTAAGGGTGT GCAAATTAAT GAAAAAATCA CTGTCCCACA TGTTTTGCAT 
GCCAAGAAAA CTACACTAAA AGCACCTGTT AACCGTAGAA TGCCTCATGT TACAAGCACT 
AGCAAGCTTT CTCCAACTAA AAGGAGCTTG TCTGAAACAG TAACTCACAG AGCCAAAATC 
ATGAAAATTG CTACTAAAAA GCGAAATAGT GTTCATGTTA CTTTTAGACC TTCTACTGAG 
TCAGTTCAGT TTTATAATCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTGCGG 
AAGCTCAGTG GCTTTAGTGG TAGTAATAGC AACATCAGCA ACCCCCACAC CAGCTCCAAC 
AGTGCTGCAG AGCTAGTAAA ACCTGGTGTG TACAGGCCTC TAGATACAAT TAGTGCAGCG 
TCAGCAAGCA GTGAAACAAT TAAGGAAACA ACAGAGATTC CCACCGCCAT ATTACAGAAA 
GAAGGAATTG CAACTAATCA GTTAGGTAGT CGTAGTACTA TTAAGTCATC AAGTCACGAG 
GCAGGACTAC CGCAGGGCTC CCTGGGTGGC GTTTATAAAA CTGTTGTCCA TGCTCTTTCG 
AAGCCGAAGG CAAATGTTTC CCCACAGAGG CAGAACAGAA TGCCACCAGA GGCTCCACTG 
AGGGATCTGT ACAGTCATGT AATGGGCTAT TTTGGAAGGA AAGCTGCAGT CAATAAAGAG 
GACATGAGCA CAAAACTGCC CCCTCTTAAT AGTGATATTG GCAGCAGCAA TGCTAATGTT 
CCTGATCTGA TGGATGAGTT TATAGCAGAG CGACTTCGAA GTGGTAATGC CTCAACTATG 
ACACGAAGAG GAAGTAGTCC AGGTAGCCTC GAAATTCCCA AAGACCTTCC TGATATTCTA 
AATAAGCAAA ACCAGATGCG TCCTATTGAT GACCCAGGTG TACCCTCAGA ATGGACTTCT 
CCTGCCAGTG CAGGGAGCAG TGATCTTATC AGCTCAGATA GTCATTCGGA TTCTTTCAGC 
GCTTTCCAAT ATGATGGCCG AAAATTTGAC AATTTTGGTT TTGGAGCGGA TACTGGGAAT 
GCATCCTCTG CTGACATGGA TTCAGGTTCT GGCCATTATC AGAGTGCTGA AGAGCAAGAG 
GTGGCTAGTC TAACCACACT ACACATCGAC TCGGAAACAA GCAGTCTTAA TCAACAAGCT 
TTCTGTACTG ATGTTGCAAC GGTTACTGGT TCAGAAAGTG CCTCTCCAGT CCATTCAGCT 
CTGGGATCCA GGTCACAGAC ACCTTCTCCT TCCACACTAA ATATAGATCA CATGGAACAG 
AAGGATCTAC AGCTCGATGA AAAGCTCCAC CACTCTGTTC TTCAGACACC AGATGACCTA 
GAAATCAGTG AATTTCCATC TGAATGCTGT AGTGTGATGG CAGGGGGCAC TCTTACAGGA 
TGGCATGCTG ATGTTGCTAC TGTAATGTGG CGAAGGATGC TGGGTATTTT GGGAGATGTC 
AACGCTATTA TGGATCCTGA AATACATGCT CAAGTTTTTG ATTACCTATG TGAACTTTGG 
CAGAATCTAG CTAAGATTAG AGATAATCTT GGCATTTCAA CTGATAACCT GACCTCCCCT 
TCTCCACCGG TTTTAATTCC TCCACTGAGA ATTCTTACAC CTTGGCTTTT TAAGGCAACC 
ATGTTAACTG ATAAATATAA ACAAGGTAAA TTACATGCCT ATAAACTTAT TTGTAATACA 
ATGAAAAGAA GACAAGATGT TTCTCCAAAT AGAGATTTTC TAACACATTT CTACAATATA 
ATGCATTGTG GATTACTTCA TATTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCACCTCAAT TTTTCTCACT TGGTTTGCCT GGTGCCACAA TGCTTATTAT GGATTTTATT 
GTAGCAGCTG GTAGAGTGGC TTCTTCAGCT TTTCTCAATG CACCAAGAGT GGAAGCACAA 
GTTCTTCTGG GATCTTTGGT TTGCTTTCCC AACTTGTATT GTGAACTGCC TGCTCTCCAT 
CCCAACATCC CTGATATTGC TGTGTCTCAA TTTACAGATG TTAAGGAACT TATAATCAAA 
ACTGTGTTAA GCTCTGCAAG AGATGAGCCC TCTGGTCCTG CACGATGTGT AGCACTTTGC 
AGTTTAGGTA TTTGGATTTG TGAAGAATTA GTCCATGAGT CTCATCATCC TCAAATTAAG 
GAAGCTCTGA ATGTAATTTG TGTTTCCTTA AAGTTTACTA ATAAAACAGT AGCCCATGTA 
GCTTGTAACA TGCTTCATAT GCTGGTTCAT TATGTACCTA GACTTCAGAT TTACCAGCCT 
GATTCTCCCT TGAAAATTAT TCAAATTCTA ATAGCCACTA TTACCCATCT TTTGCCAAGT 
ACAGAAGCAT CATCTTATGA AATGGACAAG AGGTTGGTGG TATCCTTACT TCTGTGTCTT 
CTGGACTGGA TCATGGCCTT ACCTCTTAAG ACACTGCTCC AACCAGTCCA TGCTACAGGA 
GCAGAAAATG ATAAAATAGA AAAATCTGTC CTTAACTGTA TTTATAAGGT ATTACATGGG 
TGTGTTTATG GAGCTCAGTG TTTTAGCAAT CCGAGGTATT TTCCAATAAG CCTTTCTGAT 
TTGGCATCTG TAGATTATGA TCCTTTTATG CATTTGGAAA GCCTAAAAGA GCCTGAACCT 
TTGCACTCTC CTGATTCGGA ACGATCCTCT AAACTCCAGC CAGTAACAGA AGTGAAAACT 
CAAATGCAGC AAGGATTAAT CTCCATAGCT GCCCGCACTG TTATTACACA TCTGGTGAAT 
CACTTGGGCC ACTATCCAAT GAGTGGCGGT CCTGCTATGT TAACAAGTCA GGTGTGTGAG 
AATCACGACA ATCATTACAG TGAAAGCACT GAACTTTCCC CGGAACTCTT CGAGAGTCCA 
AATATTCAGT TTTTTGTGTT AAACAATACA ACCTTAGTGT CCTGTATCCA GATCAGAGCT 
GAAGAGAGTA TACCTGGAGG AGGTTTATCT GCTGGTCTTG CATCAGCCAA TTCAAATGTC 
AGAATTATAG TACGTGATCT TTCTGGAAAA TATTCATGGG ATTCTGCCAT CCTGTATGGA 
CCACCTCTTG TAAGTGGTCT ATCAGAACCT ACATCTCTCG TACTTTCATT GTCTCACCAA 
GAGAAGCCAG AAGAACCTTC TGCATCTAAT GAATGCTTAG AAGATATAAC AGTTAAAGAT 
GGGATTTCCC TCCAGTTTAA AAGAAGTTTT AGAGAAACTG TACCAACTTG GGACACAATA 
AGAGATGAAG AAGATGTTCT TGATGAGCTT TTGCAGTATT TAGGTGTAAC CAGTCCTGAA 
TGCTTACAGA GGACTGGAGT CTCACTTAAT ATTCCTGCTC CACAGCCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT TATTAATGCT ATCCTTAAGC AGCATACAGA GGAAAAAGAA 
TTTGTTGAGA AACACTTCAA TGACTTAAAC ATGAAAGCTG CAGAACAAGA TGAACCAACC 
CCTCAGAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG 
AATTCTTGGG ACAAAAGGAG GAGTTTTCAT CTCCTGAAGA AAAATGAAAA ACTACTTAGA 
GAACTTAGGA ACTTGGACTC AAGACAGTGC CGAGAGACAC ACAAAATTGC AGTATTTTAT 
GTTGCTGAAG GACAAGAAGA CAAACATTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTC 
ATGGGAGGAC TACAAAAAAA TAAAAGCACT GGATTGACCA CACCATATTT TGCTACCTCT 
ACAGTTGAAG TAATATTTCA CGTGTCAACA AGAATGCCTT CGGATTCTGA TGACTCTTTG 
ACCAAAAAAT TGAGACATTT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT 
AGAGACTACA GGAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTTAT TGTAATATAC 
CCGATGAAAA ACCACATGTT CAGTATCCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTCT TTGATGGTGC TATTGTGAAT GGAAAGGTCC TACCTATTAT GGTTCGAGCA 
ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCGCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTATCT GCAAACGATT GTCCAGCACC ATTTAGAACC AACGACATTT 
GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ATCATTTACC ATCTGATGCC 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RALGAPA1
Entrez Gene ID	618573
Full Name	Ral GTPase activating protein catalytic alpha subunit 1
Synonyms	GARNL1
General protein information	Preferred Names Ral GTPase activating protein catalytic alpha subunit 1 Names ral GTPase-activating protein subunit alpha-1 GTPase activating Rap/RanGAP domain-like 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Mus musculus (house mouse)	Ralgapa1	NP_001003719.1
Homo sapiens (human)	RALGAPA1	NP_001269972.1
Pan troglodytes (chimpanzee)	RALGAPA1	XP_003314394.1
	RALGAPA1	XP_001088633.1
Rattus norvegicus (Norway rat)	Ralgapa1	NP_064468.2
Drosophila melanogaster (fruit fly)	CG5521	NP_001163748.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004143	XP_313024.5
Bos taurus (cattle)	RALGAPA1	XP_005222134.1
Gallus gallus (chicken)	RALGAPA1	NP_001264376.1
Danio rerio (zebrafish)	ralgapa1	NP_001139776.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RALGAPA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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