CG5521 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5521
Entrez Gene ID	43242
Full Name	CG5521 gene product from transcript CG5521-RB
Synonyms	Dmel\CG5521,EP3527,FBpp0084441
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 97D11-97D11

mRNA and Protein(s)

mRNA	Protein	Name
NM_143259.3	NP_651516.1	uncharacterized protein, isoform A
NM_001170277.2	NP_001163748.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ralgapa1	NP_001003719.1
Homo sapiens (human)	RALGAPA1	NP_001269972.1
Pan troglodytes (chimpanzee)	RALGAPA1	XP_003314394.1
	RALGAPA1	XP_001088633.1
Rattus norvegicus (Norway rat)	Ralgapa1	NP_064468.2
Drosophila melanogaster (fruit fly)	CG5521	NP_001163748.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004143	XP_313024.5
Bos taurus (cattle)	RALGAPA1	XP_005222134.1
Gallus gallus (chicken)	RALGAPA1	NP_001264376.1
Danio rerio (zebrafish)	ralgapa1	NP_001139776.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06259	NM_143259.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5521), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa08862	NM_001170277.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5521), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5521 ( NM_143259.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651516.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG5521), mRNA.

Price & Availability↑

CloneID	OFa06259
Clone ID Related Accession (Same CDS sequence)	NM_143259.3
Accession Version	NM_143259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143259.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTACAA AAAAATCGCA TGCCGACGTG AAGAAATCCA CGGCCAAGTT GCAGGACTCC 
AAGAAGGATT CCGCCTCCCG ACTGCGACAC CTACGCATGA TATTGGATAA TGTGGAACTA 
GAAGAATCGA AGTGCCTGTT CGAGACCAAC TACAGTCATG TCTACTTCAT ACTCTACGAC 
ACCTTTATTC AAGCCGAGGC GAATCTAAAA CAAAAAGTGC ACAAGGCGCA TCGCGAGGAA 
CTGGACGGTT CACTATGGCT GCTGGAGAAG ATCCTGTGCC TTCTGCCGGA ATTGTTGGCC 
CGCCGGTGGC AATGCCATTC CCTAAGTCGC ATCATGGCCA AACTATTGCA TCTGGGCAAC 
TCGCCCAAGC TGCGACGCGA AGGTGTCAGA TACTTCTTAC TGTGGTATCA GACACTAGGC 
GAAAACGCAC CAGGCTACGT GCACGCCATG TACGCGGACC TTATCCCTGG TCTTATTGTA 
CCCCAAAAAG GTGTGGTCGG TCCGGATACA GAGTTCAGTG CCTCGGACTT TCTCACTCAT 
CCAAACATGA AGGCGGACGG CGGGATGGCC TCCGTTTTTC ACGACAACGC ATTCTCACAT 
CCCGTGCAGA GTTCGGAGGT CGTTGCCCTG TTGCCACCTT CATCTAGCGA AAAATCAGCG 
CCACCAGATC CACGGGATGG CCTGGAGGTA CTGCTAAACA GCATGGTGCA CACGGCGGCC 
TGCCTAAGAT GGCGGGATAA TAGGGCACAA AAGGATCATC GGGCATTTGC CTTCCTGCTG 
CAGCGATTTA TGGATGTTTT CCTACCAGTA TTTTCACCCA ACTTCGATGT CAGTTGCTCC 
ATTTACAATC CGCGATTGGA TTTGCCCGTC ATGCGATCCA TAAACAAAAA GGAAGAGGTA 
ATGGCCTCTT GCGTTGTGGT CTTAATCAAC TGGGTATCAC GTTTCACCCA CGAGCGCTTG 
CTGAGCCACC GATTGGATTG CACCTTACAC ATCGAGGACG TGGACCAAGT GCGTCTACAC 
GGCTACCAGC AGGGTCTAAT TGTCCGGGAT GTGTTATATG TTAACCGCGA GAACATCAAC 
TTTGTGCACG AGGTGTATCG TCAGGCGTTT TTGCTTAACT TCACTTCGAA GCCGCAAATA 
GAAGCAATTC GCACAGCCAT CGCAGTTTAT CGCGACTGGA TGACAGGCGA GACGCCTCCA 
CCGTTTCTAT TGGAGCCAAA CGATGATCCA CCGCCTCCAT CGAATGCGGG TGGAACTCCC 
AGGAGTCAAC GACTTCGCAC ACCATCTTAT GTGGGCGCTA TTGCAGGCTC CAAAGATCAG 
TTGGTAGTTA GAGCTGGCCG ACAAAATGTG TTACAGGTCT TTGTGACCAA TGCGGCAAAC 
GTGTTCCTGG TGAACACGGC CAACCTGAAC ATCTGCTTTC CCACCCGCTC GCGAAGTTAT 
CGCTCCACGC CACTGGAAGA GCAGACGGAG ATCTGCAAGA AGGTGCTGAA TGTGTACCGC 
ACGATGGTTA TGAACACGGA AATGGACACG CGCACCTGGG AGCAACTGCT TATGGTTTTA 
CTGCAGGTGA CCTCAATTGT GCTACACCAA AATCAGCATA CATTGCCAAG TGGCACCAAC 
AAGAGTGCCA CTTTGGGCGG GATCCTAGGG TCAGCTATAT TCCAAACTCT CATTGTCACA 
TGGATACGAG CGCACACCAA AGTGCCTGTC AACGTGCTGC TGTGGGAAAA GTTTTTAAAT 
GTCCTGCAAT CTTTGACGCA CCGCGAGGAG CTCATTATTG AATGGAATAA GACGATTCAA 
ACTCTGACGC GTGTGTTCTC GCGATACACT TATGGCATAA ACTTGCTGGA TCTACCTTTG 
GATCGAGTGG CTGAGAGTCG CGCGGAAAAG AGACGTCGCA TTGGCAGCGT CTGGCAGGGA 
TCGGGATCAA ACAGCGCTGC TAATGGAGGA AGTGCTGCTT CTGCTGCAAT TAGCCAGAAC 
AGACAGCGGG AATCTCTGGC GGAGTCGAGT AGCAGTCGCT CGGAGGAGAC CTCGTCGCAG 
GTGCCTCTTC CAAATCACCC TCGACCTCAT CACCAGCACA CGCAATCCCA GGGAGGCACT 
GGCCATGGAA CACGACCGAT ACCTTTAACT CCCATGCTAA GTAGAAGCTA CAGCGAAGGA 
AGCCTGGCTT CAGCGGCTCG TAGTTCGAGG ATACGACGTC GACGGGCGGC CACTCCCAAG 
CCCAAGGCGC TACAGCCGTC ACAACTCGCA GAGAACCGCA TTAATCCGCA GGACTTGCGG 
CGCGCCATGT CCCTTGATTC GTTGGCGCGG AAAGGTGATG CAGAGGAAAC CGATAGCTAC 
CAGGAAGGAG ACAATGAAAG TGGAGCTGGC TCGAGGAGTC CGTCACCCAC AGCCAGCAGT 
GGAATTGAAG GTGGCTCCAT CAAGGATGCC CAACTGCAGA TTGATGCGAG CTTGGATGAT 
GCAAATTCTG GTAGTTACGG AAGTGGCAGT AACTCGGGCA GTATTTCCGG ACGTCGCTGT 
ATTATTCTAG GAGGCTCAGC AGAGGGTTGG CATCCAGATT CCACTTCCAT TATGTGGAAA 
CGCATGCTTG GCGCTCTAGG AGATGTTAAT CGCATTCCCA AAGCTGATTT ACACGCCCAG 
GTGTTTATGC ATCTGCTAGA AATGACGCAA AATCTCATCA AGATCAAGCA GAACCAAGGA 
ATATCCACGG ATAATCAGAA CACGCAACCG ATGCCACCTT TGGTACCACC CATTGGAATC 
GTGGCACCAT GGTGTTACGG ATCCCTTTCT CTGGATCGCT CTTTCAAGAA GGGCAAACTC 
TGGGCCCTTC AATTGCTCTG TTCGCTTGCG ATTCAAGGTG CTGTCAACAT GCAACAGCTG 
CCCTTGTTCT ATCATGCTCT CCATCAGCTG CTGACTGGCG AGGATCGCGA CTTGATTTAT 
GCAATTCTTA AGCACCTAGA AGGGCCTAGG CTCCTAAGTT TGCTTTTGCC AGGACACACC 
CTGCTGCTGT TGGACTTGGT CCATGCAAGT GCTATACTGC TGACTTCTCT GGAGGTCTCT 
AGGAGCACAC CAAGAGCGGA AGTTGCTGCT CTTCTGGGAT CACTGCTATG TTATCCGTCC 
TCGTTGCTGA CACGTTCCGT TCTGCAGCCC ACGCCGCAGA AGTTTGAGCT TATGGAATGT 
TCGGACTTGC AAGATCACAT CCTGAACATT GTGCTGCGAT GCGCCAGGCG AGAGCCTTCA 
GCCAAGGCAC GATGCATTGC ACTCTCTCAG CTCGGACAGT GGTTGCTAAT GCGCCTGTCT 
CAGCCACTGC CGGCTTCGAA TTCCGGAAGG GCTAACCTCT TTCAACAAGC GGTGCCGCAT 
CACAAGGATG TGCATCCTAA GGCGAGCAGT GTCTACAATC CGCGCATAAA GGAGGTGCTA 
CAAGTGCTGC TCCAGGCGTT GCAGTTTAAG CATCACACCA TTGCCATTGT AGCGGTGGAT 
TCCTTAAAGT TGTGTGCCGA GCGTGGACGC CAACTGGCGG CTATTGAAAG AGTTCCCCAG 
CTAATCATCA CTGCCATCTG CAAAGCTTTG GAAATTCAAA GCGTAACCAA GCCTAAGGAT 
TCCGACAAAG TGGTGCTGAC TTCTCTGATG TTGTGCCTGG GCGAATTCTG TATGGCTATT 
CCGGCTCCTA TTATGCTGAC GCCCTTCAAC GAACAAGGTG ATACGCTAGT GCTCCAGGTG 
CTCAGGGTAC TGTTGCAAGT TGCTTCCGGT GCTCCGCGTC ATGAGAGAGT GAAACTGACG 
GCCGATGATG ACTTTGATAT GCACATCGCA CACGACGATC TTCAAGGCGA TGGACGCCTG 
CCGGAAGCCA CCTATCAGAC CTCCGAGACC ATCCAGAAGT GCATTACAGC CATTAAGCTG 
TGTGCCAAAG CAGTGTCCAT GCATTTGGTC ACCCACATCG GTCACTTTCC GATGGGAATC 
GGAGCATCTC GTTTGAGTTC CATGGTCGAG GAGCAGGATG ACATTGGAAA TGCCGCCTAT 
GGTGGTCAGG TGGAGACGAG GCGCGATTCT GTGGAGCTTC CTTCCGTTGT AAGTGCCCAA 
AATATGCAGC TTTTCATGCT AAATTCTGGT CTGGTAGCCA GTTTTATTGA GCTGCCAACA 
TTAAAACTAC CCGGTGGAGG TATAACCGCT GGCTTGGTTA CAGCCGATAA GCAAGTTCGG 
GTCTTAATGA GGGATCTCAA TGGAAAAGCT TGCTGGGACG CTTCTATCTT GTATTCAGAA 
CCGCGCAATG CGGAGGAGCC TCCAAAAACC ACACCAAAGA TTCAACATAG CCAACCGTTG 
GATTCCATGG CGACCTCCAT GGTGACACAC ACACCAAGTC CTCGACACAC TTTGCGACAC 
CGACCGGCGG GAGTGCTGCC ATTGGCCAAG GACATGGCTC CGGATCTGGA TCAATTGGAC 
GATATGTTGG CCTATATTGG ACACACCAGT CCAGAGTGTG TGGCACCCAC TGTAAGCCAG 
CTGAATGCTC CAACGGCCAG TCCTCTGAGT GGAAACCAAG AAGCTCAGGC CATTTCGGTA 
ATTCTGAATC AACGCCTTTT GGAGCAGGAG TTTGTGACCC ACTCGACTCA GGCTCCATCG 
CCGGCATTGC GACACGCCAG CTCGAATTCC TCACTGCAGC AACCGGATCA GAGGTCACTG 
CACTCCACAA CAGCCTCCTT TGATTCCTTA CCAACCCGCA CAGAGATGCC GTTCCAATAT 
TGCCGGCTGC TCTTTTCACA TTTGGGACTA GCTGGCTGGG AGCGACGGTC TAGGACGCAC 
TTGCTTCAGA GGAGCGAAAA ACTCATGAGA GAACTGCGGA ACGTTGATCT CCAAAAATGC 
CGGGAAACCC ACAAAATGGC GGTTATTTAT GTGGCCGCCG GGCAGGAAGA TAAGGGAAGC 
ATCCTTAGGA ATACGAGTGG AAGCAGCACC TACGAGATGT TCGTTTCTGC CTTGGGTTGG 
GAAATCGATC TGGAGACACA CAACGGCTTC TTGGGCGGAT TACCACGCCA AGGATGCGGA 
GCTACAGCTC CCTATTACGC TACCCCTTTT CTCGAGGTGG TTTACCATGT GGCCACCAGG 
ATGCCCTCGG ATTCTTCGGA GGCCATGCTA CTGAAGACGC GGCATCTTGG CAACGATGAG 
GTTCACATTG TGTGGAGCGA ACACAATCGG GATTACAGGC GGGACATATT GCCCACTGAG 
TTTTGCGATG TGCTGATTGT GGTTTATCCG CTGCGGAATG GTTTGTTCCG GGTGACTGTG 
AACAGGAAGC CTGAAGTTCC ATGGTTTGGA CCATTGGCCA ACGAGAGCGT GGTAAGTGGC 
GCCTGCTTGG CCACTCTTAT TCGGGCAACC GCAATCAATG CCAGTCGAAC GAAGCGCGCT 
GCCCTGCCGC TCTACCAACA ATTCTATGAG GAGCGCAACC GGTCGTTGGA TAGCGTATCA 
TCTCGGTACA AGGAGAGCAC CACTTTCGAA GACTTTGCCA GCCGCATCTA CAATCCCATG 
CCGCTGTCTA CGCTGGGCAC ACTAAGGGAA TCCAATGCCA GCAGCTCGGC CGCACCTCTG 
GCTTCTGCAT TGCTGGATCA CAATCGTGCC TCCGTCAAAG GTTGGGTACA AGCCTCGATT 
GATTCGGGGC CCATAATGGG GATTGCGCCA TCGGCTTCAG CTGGATCCAC CGCGGCCATG 
GAGGCAGCCA CCGGAATGTC CTCCGCTTCG CCGCGTGGCC CTCGAAAACT GGGGGCTCCG 
TTCAAGAGCG TGACCAAAAA GCATTCGCTG CAGCATATCG CCGTGGGAGG CGGCGCAGGA 
GCTGGCGGTG ACACGCCGCC CGAGAGCCCA ACATTGCCGC AGCGACGCTT CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651516.1
CDS	515..6391
Translation	MFTKKSHADVKKSTAKLQDSKKDSASRLRHLRMILDNVELEESKCLFETNYSHVYFILYDTFIQAEANLKQKVHKAHREELDGSLWLLEKILCLLPELLARRWQCHSLSRIMAKLLHLGNSPKLRREGVRYFLLWYQTLGENAPGYVHAMYADLIPGLIVPQKGVVGPDTEFSASDFLTHPNMKADGGMASVFHDNAFSHPVQSSEVVALLPPSSSEKSAPPDPRDGLEVLLNSMVHTAACLRWRDNRAQKDHRAFAFLLQRFMDVFLPVFSPNFDVSCSIYNPRLDLPVMRSINKKEEVMASCVVVLINWVSRFTHERLLSHRLDCTLHIEDVDQVRLHGYQQGLIVRDVLYVNRENINFVHEVYRQAFLLNFTSKPQIEAIRTAIAVYRDWMTGETPPPFLLEPNDDPPPPSNAGGTPRSQRLRTPSYVGAIAGSKDQLVVRAGRQNVLQVFVTNAANVFLVNTANLNICFPTRSRSYRSTPLEEQTEICKKVLNVYRTMVMNTEMDTRTWEQLLMVLLQVTSIVLHQNQHTLPSGTNKSATLGGILGSAIFQTLIVTWIRAHTKVPVNVLLWEKFLNVLQSLTHREELIIEWNKTIQTLTRVFSRYTYGINLLDLPLDRVAESRAEKRRRIGSVWQGSGSNSAANGGSAASAAISQNRQRESLAESSSSRSEETSSQVPLPNHPRPHHQHTQSQGGTGHGTRPIPLTPMLSRSYSEGSLASAARSSRIRRRRAATPKPKALQPSQLAENRINPQDLRRAMSLDSLARKGDAEETDSYQEGDNESGAGSRSPSPTASSGIEGGSIKDAQLQIDASLDDANSGSYGSGSNSGSISGRRCIILGGSAEGWHPDSTSIMWKRMLGALGDVNRIPKADLHAQVFMHLLEMTQNLIKIKQNQGISTDNQNTQPMPPLVPPIGIVAPWCYGSLSLDRSFKKGKLWALQLLCSLAIQGAVNMQQLPLFYHALHQLLTGEDRDLIYAILKHLEGPRLLSLLLPGHTLLLLDLVHASAILLTSLEVSRSTPRAEVAALLGSLLCYPSSLLTRSVLQPTPQKFELMECSDLQDHILNIVLRCARREPSAKARCIALSQLGQWLLMRLSQPLPASNSGRANLFQQAVPHHKDVHPKASSVYNPRIKEVLQVLLQALQFKHHTIAIVAVDSLKLCAERGRQLAAIERVPQLIITAICKALEIQSVTKPKDSDKVVLTSLMLCLGEFCMAIPAPIMLTPFNEQGDTLVLQVLRVLLQVASGAPRHERVKLTADDDFDMHIAHDDLQGDGRLPEATYQTSETIQKCITAIKLCAKAVSMHLVTHIGHFPMGIGASRLSSMVEEQDDIGNAAYGGQVETRRDSVELPSVVSAQNMQLFMLNSGLVASFIELPTLKLPGGGITAGLVTADKQVRVLMRDLNGKACWDASILYSEPRNAEEPPKTTPKIQHSQPLDSMATSMVTHTPSPRHTLRHRPAGVLPLAKDMAPDLDQLDDMLAYIGHTSPECVAPTVSQLNAPTASPLSGNQEAQAISVILNQRLLEQEFVTHSTQAPSPALRHASSNSSLQQPDQRSLHSTTASFDSLPTRTEMPFQYCRLLFSHLGLAGWERRSRTHLLQRSEKLMRELRNVDLQKCRETHKMAVIYVAAGQEDKGSILRNTSGSSTYEMFVSALGWEIDLETHNGFLGGLPRQGCGATAPYYATPFLEVVYHVATRMPSDSSEAMLLKTRHLGNDEVHIVWSEHNRDYRRDILPTEFCDVLIVVYPLRNGLFRVTVNRKPEVPWFGPLANESVVSGACLATLIRATAINASRTKRAALPLYQQFYEERNRSLDSVSSRYKESTTFEDFASRIYNPMPLSTLGTLRESNASSSAAPLASALLDHNRASVKGWVQASIDSGPIMGIAPSASAGSTAAMEAATGMSSASPRGPRKLGAPFKSVTKKHSLQHIAVGGGAGAGGDTPPESPTLPQRRFK

Target ORF information:

RefSeq Version	NM_143259.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5521), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143259.3

ORF Insert Sequence:

ATGTTTACAA AAAAATCGCA TGCCGACGTG AAGAAATCCA CGGCCAAGTT GCAGGACTCC 
AAGAAGGATT CCGCCTCCCG ACTGCGACAC CTACGCATGA TATTGGATAA TGTGGAACTA 
GAAGAATCGA AGTGCCTGTT CGAGACCAAC TACAGTCATG TCTACTTCAT ACTCTACGAC 
ACCTTTATTC AAGCCGAGGC GAATCTAAAA CAAAAAGTGC ACAAGGCGCA TCGCGAGGAA 
CTGGACGGTT CACTATGGCT GCTGGAGAAG ATCCTGTGCC TTCTGCCGGA ATTGTTGGCC 
CGCCGGTGGC AATGCCATTC CCTAAGTCGC ATCATGGCCA AACTATTGCA TCTGGGCAAC 
TCGCCCAAGC TGCGACGCGA AGGTGTCAGA TACTTCTTAC TGTGGTATCA GACACTAGGC 
GAAAACGCAC CAGGCTACGT GCACGCCATG TACGCGGACC TTATCCCTGG TCTTATTGTA 
CCCCAAAAAG GTGTGGTCGG TCCGGATACA GAGTTCAGTG CCTCGGACTT TCTCACTCAT 
CCAAACATGA AGGCGGACGG CGGGATGGCC TCCGTTTTTC ACGACAACGC ATTCTCACAT 
CCCGTGCAGA GTTCGGAGGT CGTTGCCCTG TTGCCACCTT CATCTAGCGA AAAATCAGCG 
CCACCAGATC CACGGGATGG CCTGGAGGTA CTGCTAAACA GCATGGTGCA CACGGCGGCC 
TGCCTAAGAT GGCGGGATAA TAGGGCACAA AAGGATCATC GGGCATTTGC CTTCCTGCTG 
CAGCGATTTA TGGATGTTTT CCTACCAGTA TTTTCACCCA ACTTCGATGT CAGTTGCTCC 
ATTTACAATC CGCGATTGGA TTTGCCCGTC ATGCGATCCA TAAACAAAAA GGAAGAGGTA 
ATGGCCTCTT GCGTTGTGGT CTTAATCAAC TGGGTATCAC GTTTCACCCA CGAGCGCTTG 
CTGAGCCACC GATTGGATTG CACCTTACAC ATCGAGGACG TGGACCAAGT GCGTCTACAC 
GGCTACCAGC AGGGTCTAAT TGTCCGGGAT GTGTTATATG TTAACCGCGA GAACATCAAC 
TTTGTGCACG AGGTGTATCG TCAGGCGTTT TTGCTTAACT TCACTTCGAA GCCGCAAATA 
GAAGCAATTC GCACAGCCAT CGCAGTTTAT CGCGACTGGA TGACAGGCGA GACGCCTCCA 
CCGTTTCTAT TGGAGCCAAA CGATGATCCA CCGCCTCCAT CGAATGCGGG TGGAACTCCC 
AGGAGTCAAC GACTTCGCAC ACCATCTTAT GTGGGCGCTA TTGCAGGCTC CAAAGATCAG 
TTGGTAGTTA GAGCTGGCCG ACAAAATGTG TTACAGGTCT TTGTGACCAA TGCGGCAAAC 
GTGTTCCTGG TGAACACGGC CAACCTGAAC ATCTGCTTTC CCACCCGCTC GCGAAGTTAT 
CGCTCCACGC CACTGGAAGA GCAGACGGAG ATCTGCAAGA AGGTGCTGAA TGTGTACCGC 
ACGATGGTTA TGAACACGGA AATGGACACG CGCACCTGGG AGCAACTGCT TATGGTTTTA 
CTGCAGGTGA CCTCAATTGT GCTACACCAA AATCAGCATA CATTGCCAAG TGGCACCAAC 
AAGAGTGCCA CTTTGGGCGG GATCCTAGGG TCAGCTATAT TCCAAACTCT CATTGTCACA 
TGGATACGAG CGCACACCAA AGTGCCTGTC AACGTGCTGC TGTGGGAAAA GTTTTTAAAT 
GTCCTGCAAT CTTTGACGCA CCGCGAGGAG CTCATTATTG AATGGAATAA GACGATTCAA 
ACTCTGACGC GTGTGTTCTC GCGATACACT TATGGCATAA ACTTGCTGGA TCTACCTTTG 
GATCGAGTGG CTGAGAGTCG CGCGGAAAAG AGACGTCGCA TTGGCAGCGT CTGGCAGGGA 
TCGGGATCAA ACAGCGCTGC TAATGGAGGA AGTGCTGCTT CTGCTGCAAT TAGCCAGAAC 
AGACAGCGGG AATCTCTGGC GGAGTCGAGT AGCAGTCGCT CGGAGGAGAC CTCGTCGCAG 
GTGCCTCTTC CAAATCACCC TCGACCTCAT CACCAGCACA CGCAATCCCA GGGAGGCACT 
GGCCATGGAA CACGACCGAT ACCTTTAACT CCCATGCTAA GTAGAAGCTA CAGCGAAGGA 
AGCCTGGCTT CAGCGGCTCG TAGTTCGAGG ATACGACGTC GACGGGCGGC CACTCCCAAG 
CCCAAGGCGC TACAGCCGTC ACAACTCGCA GAGAACCGCA TTAATCCGCA GGACTTGCGG 
CGCGCCATGT CCCTTGATTC GTTGGCGCGG AAAGGTGATG CAGAGGAAAC CGATAGCTAC 
CAGGAAGGAG ACAATGAAAG TGGAGCTGGC TCGAGGAGTC CGTCACCCAC AGCCAGCAGT 
GGAATTGAAG GTGGCTCCAT CAAGGATGCC CAACTGCAGA TTGATGCGAG CTTGGATGAT 
GCAAATTCTG GTAGTTACGG AAGTGGCAGT AACTCGGGCA GTATTTCCGG ACGTCGCTGT 
ATTATTCTAG GAGGCTCAGC AGAGGGTTGG CATCCAGATT CCACTTCCAT TATGTGGAAA 
CGCATGCTTG GCGCTCTAGG AGATGTTAAT CGCATTCCCA AAGCTGATTT ACACGCCCAG 
GTGTTTATGC ATCTGCTAGA AATGACGCAA AATCTCATCA AGATCAAGCA GAACCAAGGA 
ATATCCACGG ATAATCAGAA CACGCAACCG ATGCCACCTT TGGTACCACC CATTGGAATC 
GTGGCACCAT GGTGTTACGG ATCCCTTTCT CTGGATCGCT CTTTCAAGAA GGGCAAACTC 
TGGGCCCTTC AATTGCTCTG TTCGCTTGCG ATTCAAGGTG CTGTCAACAT GCAACAGCTG 
CCCTTGTTCT ATCATGCTCT CCATCAGCTG CTGACTGGCG AGGATCGCGA CTTGATTTAT 
GCAATTCTTA AGCACCTAGA AGGGCCTAGG CTCCTAAGTT TGCTTTTGCC AGGACACACC 
CTGCTGCTGT TGGACTTGGT CCATGCAAGT GCTATACTGC TGACTTCTCT GGAGGTCTCT 
AGGAGCACAC CAAGAGCGGA AGTTGCTGCT CTTCTGGGAT CACTGCTATG TTATCCGTCC 
TCGTTGCTGA CACGTTCCGT TCTGCAGCCC ACGCCGCAGA AGTTTGAGCT TATGGAATGT 
TCGGACTTGC AAGATCACAT CCTGAACATT GTGCTGCGAT GCGCCAGGCG AGAGCCTTCA 
GCCAAGGCAC GATGCATTGC ACTCTCTCAG CTCGGACAGT GGTTGCTAAT GCGCCTGTCT 
CAGCCACTGC CGGCTTCGAA TTCCGGAAGG GCTAACCTCT TTCAACAAGC GGTGCCGCAT 
CACAAGGATG TGCATCCTAA GGCGAGCAGT GTCTACAATC CGCGCATAAA GGAGGTGCTA 
CAAGTGCTGC TCCAGGCGTT GCAGTTTAAG CATCACACCA TTGCCATTGT AGCGGTGGAT 
TCCTTAAAGT TGTGTGCCGA GCGTGGACGC CAACTGGCGG CTATTGAAAG AGTTCCCCAG 
CTAATCATCA CTGCCATCTG CAAAGCTTTG GAAATTCAAA GCGTAACCAA GCCTAAGGAT 
TCCGACAAAG TGGTGCTGAC TTCTCTGATG TTGTGCCTGG GCGAATTCTG TATGGCTATT 
CCGGCTCCTA TTATGCTGAC GCCCTTCAAC GAACAAGGTG ATACGCTAGT GCTCCAGGTG 
CTCAGGGTAC TGTTGCAAGT TGCTTCCGGT GCTCCGCGTC ATGAGAGAGT GAAACTGACG 
GCCGATGATG ACTTTGATAT GCACATCGCA CACGACGATC TTCAAGGCGA TGGACGCCTG 
CCGGAAGCCA CCTATCAGAC CTCCGAGACC ATCCAGAAGT GCATTACAGC CATTAAGCTG 
TGTGCCAAAG CAGTGTCCAT GCATTTGGTC ACCCACATCG GTCACTTTCC GATGGGAATC 
GGAGCATCTC GTTTGAGTTC CATGGTCGAG GAGCAGGATG ACATTGGAAA TGCCGCCTAT 
GGTGGTCAGG TGGAGACGAG GCGCGATTCT GTGGAGCTTC CTTCCGTTGT AAGTGCCCAA 
AATATGCAGC TTTTCATGCT AAATTCTGGT CTGGTAGCCA GTTTTATTGA GCTGCCAACA 
TTAAAACTAC CCGGTGGAGG TATAACCGCT GGCTTGGTTA CAGCCGATAA GCAAGTTCGG 
GTCTTAATGA GGGATCTCAA TGGAAAAGCT TGCTGGGACG CTTCTATCTT GTATTCAGAA 
CCGCGCAATG CGGAGGAGCC TCCAAAAACC ACACCAAAGA TTCAACATAG CCAACCGTTG 
GATTCCATGG CGACCTCCAT GGTGACACAC ACACCAAGTC CTCGACACAC TTTGCGACAC 
CGACCGGCGG GAGTGCTGCC ATTGGCCAAG GACATGGCTC CGGATCTGGA TCAATTGGAC 
GATATGTTGG CCTATATTGG ACACACCAGT CCAGAGTGTG TGGCACCCAC TGTAAGCCAG 
CTGAATGCTC CAACGGCCAG TCCTCTGAGT GGAAACCAAG AAGCTCAGGC CATTTCGGTA 
ATTCTGAATC AACGCCTTTT GGAGCAGGAG TTTGTGACCC ACTCGACTCA GGCTCCATCG 
CCGGCATTGC GACACGCCAG CTCGAATTCC TCACTGCAGC AACCGGATCA GAGGTCACTG 
CACTCCACAA CAGCCTCCTT TGATTCCTTA CCAACCCGCA CAGAGATGCC GTTCCAATAT 
TGCCGGCTGC TCTTTTCACA TTTGGGACTA GCTGGCTGGG AGCGACGGTC TAGGACGCAC 
TTGCTTCAGA GGAGCGAAAA ACTCATGAGA GAACTGCGGA ACGTTGATCT CCAAAAATGC 
CGGGAAACCC ACAAAATGGC GGTTATTTAT GTGGCCGCCG GGCAGGAAGA TAAGGGAAGC 
ATCCTTAGGA ATACGAGTGG AAGCAGCACC TACGAGATGT TCGTTTCTGC CTTGGGTTGG 
GAAATCGATC TGGAGACACA CAACGGCTTC TTGGGCGGAT TACCACGCCA AGGATGCGGA 
GCTACAGCTC CCTATTACGC TACCCCTTTT CTCGAGGTGG TTTACCATGT GGCCACCAGG 
ATGCCCTCGG ATTCTTCGGA GGCCATGCTA CTGAAGACGC GGCATCTTGG CAACGATGAG 
GTTCACATTG TGTGGAGCGA ACACAATCGG GATTACAGGC GGGACATATT GCCCACTGAG 
TTTTGCGATG TGCTGATTGT GGTTTATCCG CTGCGGAATG GTTTGTTCCG GGTGACTGTG 
AACAGGAAGC CTGAAGTTCC ATGGTTTGGA CCATTGGCCA ACGAGAGCGT GGTAAGTGGC 
GCCTGCTTGG CCACTCTTAT TCGGGCAACC GCAATCAATG CCAGTCGAAC GAAGCGCGCT 
GCCCTGCCGC TCTACCAACA ATTCTATGAG GAGCGCAACC GGTCGTTGGA TAGCGTATCA 
TCTCGGTACA AGGAGAGCAC CACTTTCGAA GACTTTGCCA GCCGCATCTA CAATCCCATG 
CCGCTGTCTA CGCTGGGCAC ACTAAGGGAA TCCAATGCCA GCAGCTCGGC CGCACCTCTG 
GCTTCTGCAT TGCTGGATCA CAATCGTGCC TCCGTCAAAG GTTGGGTACA AGCCTCGATT 
GATTCGGGGC CCATAATGGG GATTGCGCCA TCGGCTTCAG CTGGATCCAC CGCGGCCATG 
GAGGCAGCCA CCGGAATGTC CTCCGCTTCG CCGCGTGGCC CTCGAAAACT GGGGGCTCCG 
TTCAAGAGCG TGACCAAAAA GCATTCGCTG CAGCATATCG CCGTGGGAGG CGGCGCAGGA 
GCTGGCGGTG ACACGCCGCC CGAGAGCCCA ACATTGCCGC AGCGACGCTT CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5521 ( NM_001170277.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163748.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG5521), mRNA.

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CloneID	OFa08862
Clone ID Related Accession (Same CDS sequence)	NM_001170277.2
Accession Version	NM_001170277.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170277.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTACAA AAAAATCGCA TGCCGACGTG AAGAAATCCA CGGCCAAGTT GCAGGACTCC 
AAGAAGGATT CCGCCTCCCG ACTGCGACAC CTACGCATGA TATTGGATAA TGTGGAACTA 
GAAGAATCGA AGTGCCTGTT CGAGACCAAC TACAGTCATG TCTACTTCAT ACTCTACGAC 
ACCTTTATTC AAGCCGAGGC GAATCTAAAA CAAAAAGAAC TTCCGTTTCA CATTGTGCAC 
AAGGCGCATC GCGAGGAACT GGACGGTTCA CTATGGCTGC TGGAGAAGAT CCTGTGCCTT 
CTGCCGGAAT TGTTGGCCCG CCGGTGGCAA TGCCATTCCC TAAGTCGCAT CATGGCCAAA 
CTATTGCATC TGGGCAACTC GCCCAAGCTG CGACGCGAAG GTGTCAGATA CTTCTTACTG 
TGGTATCAGA CACTAGGCGA AAACGCACCA GGCTACGTGC ACGCCATGTA CGCGGACCTT 
ATCCCTGGTC TTATTGTACC CCAAAAAGGT GTGGTCGGTC CGGATACAGA GTTCAGTGCC 
TCGGACTTTC TCACTCATCC AAACATGAAG GCGGACGGCG GGATGGCCTC CGTTTTTCAC 
GACAACGCAT TCTCACATCC CGTGCAGAGT TCGGAGGTCG TTGCCCTGTT GCCACCTTCA 
TCTAGCGAAA AATCAGCGCC ACCAGATCCA CGGGATGGCC TGGAGGTACT GCTAAACAGC 
ATGGTGCACA CGGCGGCCTG CCTAAGATGG CGGGATAATA GGGCACAAAA GGATCATCGG 
GCATTTGCCT TCCTGCTGCA GCGATTTATG GATGTTTTCC TACCAGTATT TTCACCCAAC 
TTCGATGTCA GTTGCTCCAT TTACAATCCG CGATTGGATT TGCCCGTCAT GCGATCCATA 
AACAAAAAGG AAGAGGTAAT GGCCTCTTGC GTTGTGGTCT TAATCAACTG GGTATCACGT 
TTCACCCACG AGCGCTTGCT GAGCCACCGA TTGGATTGCA CCTTACACAT CGAGGACGTG 
GACCAAGTGC GTCTACACGG CTACCAGCAG GGTCTAATTG TCCGGGATGT GTTATATGTT 
AACCGCGAGA ACATCAACTT TGTGCACGAG GTGTATCGTC AGGCGTTTTT GCTTAACTTC 
ACTTCGAAGC CGCAAATAGA AGCAATTCGC ACAGCCATCG CAGTTTATCG CGACTGGATG 
ACAGGCGAGA CGCCTCCACC GTTTCTATTG GAGCCAAACG ATGATCCACC GCCTCCATCG 
AATGCGGGTG GAACTCCCAG GAGTCAACGA CTTCGCACAC CATCTTATGT GGGCGCTATT 
GCAGGCTCCA AAGATCAGTT GGTAGTTAGA GCTGGCCGAC AAAATGTGTT ACAGGTCTTT 
GTGACCAATG CGGCAAACGT GTTCCTGGTG AACACGGCCA ACCTGAACAT CTGCTTTCCC 
ACCCGCTCGC GAAGTTATCG CTCCACGCCA CTGGAAGAGC AGACGGAGAT CTGCAAGAAG 
GTGCTGAATG TGTACCGCAC GATGGTTATG AACACGGAAA TGGACACGCG CACCTGGGAG 
CAACTGCTTA TGGTTTTACT GCAGGTGACC TCAATTGTGC TACACCAAAA TCAGCATACA 
TTGCCAAGTG GCACCAACAA GAGTGCCACT TTGGGCGGGA TCCTAGGGTC AGCTATATTC 
CAAACTCTCA TTGTCACATG GATACGAGCG CACACCAAAG TGCCTGTCAA CGTGCTGCTG 
TGGGAAAAGT TTTTAAATGT CCTGCAATCT TTGACGCACC GCGAGGAGCT CATTATTGAA 
TGGAATAAGA CGATTCAAAC TCTGACGCGT GTGTTCTCGC GATACACTTA TGGCATAAAC 
TTGCTGGATC TACCTTTGGA TCGAGTGGCT GAGAGTCGCG CGGAAAAGAG ACGTCGCATT 
GGCAGCGTCT GGCAGGGATC GGGATCAAAC AGCGCTGCTA ATGGAGGAAG TGCTGCTTCT 
GCTGCAATTA GCCAGAACAG ACAGCGGGAA TCTCTGGCGG AGTCGAGTAG CAGTCGCTCG 
GAGGAGACCT CGTCGCAGGT GCCTCTTCCA AATCACCCTC GACCTCATCA CCAGCACACG 
CAATCCCAGG GAGGCACTGG CCATGGAACA CGACCGATAC CTTTAACTCC CATGCTAAGT 
AGAAGCTACA GCGAAGGAAG CCTGGCTTCA GCGGCTCGTA GTTCGAGGAT ACGACGTCGA 
CGGGCGGCCA CTCCCAAGCC CAAGGCGCTA CAGCCGTCAC AACTCGCAGA GAACCGCATT 
AATCCGCAGG ACTTGCGGCG CGCCATGTCC CTTGATTCGT TGGCGCGGAA AGGTGATGCA 
GAGGAAACCG ATAGCTACCA GGAAGGAGAC AATGAAAGTG GAGCTGGCTC GAGGAGTCCG 
TCACCCACAG CCAGCAGTGG AATTGAAGGT GGCTCCATCA AGGATGCCCA ACTGCAGATT 
GATGCGAGCT TGGATGATGC AAATTCTGGT AGTTACGGAA GTGGCAGTAA CTCGGGCAGT 
ATTTCCGGAC GTCGCTGTAT TATTCTAGGA GGCTCAGCAG AGGGTTGGCA TCCAGATTCC 
ACTTCCATTA TGTGGAAACG CATGCTTGGC GCTCTAGGAG ATGTTAATCG CATTCCCAAA 
GCTGATTTAC ACGCCCAGGT GTTTATGCAT CTGCTAGAAA TGACGCAAAA TCTCATCAAG 
ATCAAGCAGA ACCAAGGAAT ATCCACGGAT AATCAGAACA CGCAACCGAT GCCACCTTTG 
GTACCACCCA TTGGAATCGT GGCACCATGG TGTTACGGAT CCCTTTCTCT GGATCGCTCT 
TTCAAGAAGG GCAAACTCTG GGCCCTTCAA TTGCTCTGTT CGCTTGCGAT TCAAGGTGCT 
GTCAACATGC AACAGCTGCC CTTGTTCTAT CATGCTCTCC ATCAGCTGCT GACTGGCGAG 
GATCGCGACT TGATTTATGC AATTCTTAAG CACCTAGAAG GGCCTAGGCT CCTAAGTTTG 
CTTTTGCCAG GACACACCCT GCTGCTGTTG GACTTGGTCC ATGCAAGTGC TATACTGCTG 
ACTTCTCTGG AGGTCTCTAG GAGCACACCA AGAGCGGAAG TTGCTGCTCT TCTGGGATCA 
CTGCTATGTT ATCCGTCCTC GTTGCTGACA CGTTCCGTTC TGCAGCCCAC GCCGCAGAAG 
TTTGAGCTTA TGGAATGTTC GGACTTGCAA GATCACATCC TGAACATTGT GCTGCGATGC 
GCCAGGCGAG AGCCTTCAGC CAAGGCACGA TGCATTGCAC TCTCTCAGCT CGGACAGTGG 
TTGCTAATGC GCCTGTCTCA GCCACTGCCG GCTTCGAATT CCGGAAGGGC TAACCTCTTT 
CAACAAGCGG TGCCGCATCA CAAGGATGTG CATCCTAAGG CGAGCAGTGT CTACAATCCG 
CGCATAAAGG AGGTGCTACA AGTGCTGCTC CAGGCGTTGC AGTTTAAGCA TCACACCATT 
GCCATTGTAG CGGTGGATTC CTTAAAGTTG TGTGCCGAGC GTGGACGCCA ACTGGCGGCT 
ATTGAAAGAG TTCCCCAGCT AATCATCACT GCCATCTGCA AAGCTTTGGA AATTCAAAGC 
GTAACCAAGC CTAAGGATTC CGACAAAGTG GTGCTGACTT CTCTGATGTT GTGCCTGGGC 
GAATTCTGTA TGGCTATTCC GGCTCCTATT ATGCTGACGC CCTTCAACGA ACAAGGTGAT 
ACGCTAGTGC TCCAGGTGCT CAGGGTACTG TTGCAAGTTG CTTCCGGTGC TCCGCGTCAT 
GAGAGAGTGA AACTGACGGC CGATGATGAC TTTGATATGC ACATCGCACA CGACGATCTT 
CAAGGCGATG GACGCCTGCC GGAAGCCACC TATCAGACCT CCGAGACCAT CCAGAAGTGC 
ATTACAGCCA TTAAGCTGTG TGCCAAAGCA GTGTCCATGC ATTTGGTCAC CCACATCGGT 
CACTTTCCGA TGGGAATCGG AGCATCTCGT TTGAGTTCCA TGGTCGAGGA GCAGGATGAC 
ATTGGAAATG CCGCCTATGG TGGTCAGGTG GAGACGAGGC GCGATTCTGT GGAGCTTCCT 
TCCGTTGTAA GTGCCCAAAA TATGCAGCTT TTCATGCTAA ATTCTGGTCT GGTAGCCAGT 
TTTATTGAGC TGCCAACATT AAAACTACCC GGTGGAGGTA TAACCGCTGG CTTGGTTACA 
GCCGATAAGC AAGTTCGGGT CTTAATGAGG GATCTCAATG GAAAAGCTTG CTGGGACGCT 
TCTATCTTGT ATTCAGAACC GCGCAATGCG GAGGAGCCTC CAAAAACCAC ACCAAAGATT 
CAACATAGCC AACCGTTGGA TTCCATGGCG ACCTCCATGG TGACACACAC ACCAAGTCCT 
CGACACACTT TGCGACACCG ACCGGCGGGA GTGCTGCCAT TGGCCAAGGA CATGGCTCCG 
GATCTGGATC AATTGGACGA TATGTTGGCC TATATTGGAC ACACCAGTCC AGAGTGTGTG 
GCACCCACTG TAAGCCAGCT GAATGCTCCA ACGGCCAGTC CTCTGAGTGG AAACCAAGAA 
GCTCAGGCCA TTTCGGTAAT TCTGAATCAA CGCCTTTTGG AGCAGGAGTT TGTGACCCAC 
TCGACTCAGG CTCCATCGCC GGCATTGCGA CACGCCAGCT CGAATTCCTC ACTGCAGCAA 
CCGGATCAGA GGTCACTGCA CTCCACAACA GCCTCCTTTG ATTCCTTACC AACCCGCACA 
GAGATGCCGT TCCAATATTG CCGGCTGCTC TTTTCACATT TGGGACTAGC TGGCTGGGAG 
CGACGGTCTA GGACGCACTT GCTTCAGAGG AGCGAAAAAC TCATGAGAGA ACTGCGGAAC 
GTTGATCTCC AAAAATGCCG GGAAACCCAC AAAATGGCGG TTATTTATGT GGCCGCCGGG 
CAGGAAGATA AGGGAAGCAT CCTTAGGAAT ACGAGTGGAA GCAGCACCTA CGAGATGTTC 
GTTTCTGCCT TGGGTTGGGA AATCGATCTG GAGACACACA ACGGCTTCTT GGGCGGATTA 
CCACGCCAAG GATGCGGAGC TACAGCTCCC TATTACGCTA CCCCTTTTCT CGAGGTGGTT 
TACCATGTGG CCACCAGGAT GCCCTCGGAT TCTTCGGAGG CCATGCTACT GAAGACGCGG 
CATCTTGGCA ACGATGAGGT TCACATTGTG TGGAGCGAAC ACAATCGGGA TTACAGGCGG 
GACATATTGC CCACTGAGTT TTGCGATGTG CTGATTGTGG TTTATCCGCT GCGGAATGGT 
TTGTTCCGGG TGACTGTGAA CAGGAAGCCT GAAGTTCCAT GGTTTGGACC ATTGGCCAAC 
GAGAGCGTGG TAAGTGGCGC CTGCTTGGCC ACTCTTATTC GGGCAACCGC AATCAATGCC 
AGTCGAACGA AGCGCGCTGC CCTGCCGCTC TACCAACAAT TCTATGAGGA GCGCAACCGG 
TCGTTGGATA GCGTATCATC TCGGTACAAG GAGAGCACCA CTTTCGAAGA CTTTGCCAGC 
CGCATCTACA ATCCCATGCC GCTGTCTACG CTGGGCACAC TAAGGGAATC CAATGCCAGC 
AGCTCGGCCG CACCTCTGGC TTCTGCATTG CTGGATCACA ATCGTGCCTC CGTCAAAGGT 
TGGGTACAAG CCTCGATTGA TTCGGGGCCC ATAATGGGGA TTGCGCCATC GGCTTCAGCT 
GGATCCACCG CGGCCATGGA GGCAGCCACC GGAATGTCCT CCGCTTCGCC GCGTGGCCCT 
CGAAAACTGG GGGCTCCGTT CAAGAGCGTG ACCAAAAAGC ATTCGCTGCA GCATATCGCC 
GTGGGAGGCG GCGCAGGAGC TGGCGGTGAC ACGCCGCCCG AGAGCCCAAC ATTGCCGCAG 
CGACGCTTCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163748.1
CDS	515..6409
Translation	MFTKKSHADVKKSTAKLQDSKKDSASRLRHLRMILDNVELEESKCLFETNYSHVYFILYDTFIQAEANLKQKELPFHIVHKAHREELDGSLWLLEKILCLLPELLARRWQCHSLSRIMAKLLHLGNSPKLRREGVRYFLLWYQTLGENAPGYVHAMYADLIPGLIVPQKGVVGPDTEFSASDFLTHPNMKADGGMASVFHDNAFSHPVQSSEVVALLPPSSSEKSAPPDPRDGLEVLLNSMVHTAACLRWRDNRAQKDHRAFAFLLQRFMDVFLPVFSPNFDVSCSIYNPRLDLPVMRSINKKEEVMASCVVVLINWVSRFTHERLLSHRLDCTLHIEDVDQVRLHGYQQGLIVRDVLYVNRENINFVHEVYRQAFLLNFTSKPQIEAIRTAIAVYRDWMTGETPPPFLLEPNDDPPPPSNAGGTPRSQRLRTPSYVGAIAGSKDQLVVRAGRQNVLQVFVTNAANVFLVNTANLNICFPTRSRSYRSTPLEEQTEICKKVLNVYRTMVMNTEMDTRTWEQLLMVLLQVTSIVLHQNQHTLPSGTNKSATLGGILGSAIFQTLIVTWIRAHTKVPVNVLLWEKFLNVLQSLTHREELIIEWNKTIQTLTRVFSRYTYGINLLDLPLDRVAESRAEKRRRIGSVWQGSGSNSAANGGSAASAAISQNRQRESLAESSSSRSEETSSQVPLPNHPRPHHQHTQSQGGTGHGTRPIPLTPMLSRSYSEGSLASAARSSRIRRRRAATPKPKALQPSQLAENRINPQDLRRAMSLDSLARKGDAEETDSYQEGDNESGAGSRSPSPTASSGIEGGSIKDAQLQIDASLDDANSGSYGSGSNSGSISGRRCIILGGSAEGWHPDSTSIMWKRMLGALGDVNRIPKADLHAQVFMHLLEMTQNLIKIKQNQGISTDNQNTQPMPPLVPPIGIVAPWCYGSLSLDRSFKKGKLWALQLLCSLAIQGAVNMQQLPLFYHALHQLLTGEDRDLIYAILKHLEGPRLLSLLLPGHTLLLLDLVHASAILLTSLEVSRSTPRAEVAALLGSLLCYPSSLLTRSVLQPTPQKFELMECSDLQDHILNIVLRCARREPSAKARCIALSQLGQWLLMRLSQPLPASNSGRANLFQQAVPHHKDVHPKASSVYNPRIKEVLQVLLQALQFKHHTIAIVAVDSLKLCAERGRQLAAIERVPQLIITAICKALEIQSVTKPKDSDKVVLTSLMLCLGEFCMAIPAPIMLTPFNEQGDTLVLQVLRVLLQVASGAPRHERVKLTADDDFDMHIAHDDLQGDGRLPEATYQTSETIQKCITAIKLCAKAVSMHLVTHIGHFPMGIGASRLSSMVEEQDDIGNAAYGGQVETRRDSVELPSVVSAQNMQLFMLNSGLVASFIELPTLKLPGGGITAGLVTADKQVRVLMRDLNGKACWDASILYSEPRNAEEPPKTTPKIQHSQPLDSMATSMVTHTPSPRHTLRHRPAGVLPLAKDMAPDLDQLDDMLAYIGHTSPECVAPTVSQLNAPTASPLSGNQEAQAISVILNQRLLEQEFVTHSTQAPSPALRHASSNSSLQQPDQRSLHSTTASFDSLPTRTEMPFQYCRLLFSHLGLAGWERRSRTHLLQRSEKLMRELRNVDLQKCRETHKMAVIYVAAGQEDKGSILRNTSGSSTYEMFVSALGWEIDLETHNGFLGGLPRQGCGATAPYYATPFLEVVYHVATRMPSDSSEAMLLKTRHLGNDEVHIVWSEHNRDYRRDILPTEFCDVLIVVYPLRNGLFRVTVNRKPEVPWFGPLANESVVSGACLATLIRATAINASRTKRAALPLYQQFYEERNRSLDSVSSRYKESTTFEDFASRIYNPMPLSTLGTLRESNASSSAAPLASALLDHNRASVKGWVQASIDSGPIMGIAPSASAGSTAAMEAATGMSSASPRGPRKLGAPFKSVTKKHSLQHIAVGGGAGAGGDTPPESPTLPQRRFK

Target ORF information:

RefSeq Version	NM_001170277.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5521), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170277.2

ORF Insert Sequence:

ATGTTTACAA AAAAATCGCA TGCCGACGTG AAGAAATCCA CGGCCAAGTT GCAGGACTCC 
AAGAAGGATT CCGCCTCCCG ACTGCGACAC CTACGCATGA TATTGGATAA TGTGGAACTA 
GAAGAATCGA AGTGCCTGTT CGAGACCAAC TACAGTCATG TCTACTTCAT ACTCTACGAC 
ACCTTTATTC AAGCCGAGGC GAATCTAAAA CAAAAAGAAC TTCCGTTTCA CATTGTGCAC 
AAGGCGCATC GCGAGGAACT GGACGGTTCA CTATGGCTGC TGGAGAAGAT CCTGTGCCTT 
CTGCCGGAAT TGTTGGCCCG CCGGTGGCAA TGCCATTCCC TAAGTCGCAT CATGGCCAAA 
CTATTGCATC TGGGCAACTC GCCCAAGCTG CGACGCGAAG GTGTCAGATA CTTCTTACTG 
TGGTATCAGA CACTAGGCGA AAACGCACCA GGCTACGTGC ACGCCATGTA CGCGGACCTT 
ATCCCTGGTC TTATTGTACC CCAAAAAGGT GTGGTCGGTC CGGATACAGA GTTCAGTGCC 
TCGGACTTTC TCACTCATCC AAACATGAAG GCGGACGGCG GGATGGCCTC CGTTTTTCAC 
GACAACGCAT TCTCACATCC CGTGCAGAGT TCGGAGGTCG TTGCCCTGTT GCCACCTTCA 
TCTAGCGAAA AATCAGCGCC ACCAGATCCA CGGGATGGCC TGGAGGTACT GCTAAACAGC 
ATGGTGCACA CGGCGGCCTG CCTAAGATGG CGGGATAATA GGGCACAAAA GGATCATCGG 
GCATTTGCCT TCCTGCTGCA GCGATTTATG GATGTTTTCC TACCAGTATT TTCACCCAAC 
TTCGATGTCA GTTGCTCCAT TTACAATCCG CGATTGGATT TGCCCGTCAT GCGATCCATA 
AACAAAAAGG AAGAGGTAAT GGCCTCTTGC GTTGTGGTCT TAATCAACTG GGTATCACGT 
TTCACCCACG AGCGCTTGCT GAGCCACCGA TTGGATTGCA CCTTACACAT CGAGGACGTG 
GACCAAGTGC GTCTACACGG CTACCAGCAG GGTCTAATTG TCCGGGATGT GTTATATGTT 
AACCGCGAGA ACATCAACTT TGTGCACGAG GTGTATCGTC AGGCGTTTTT GCTTAACTTC 
ACTTCGAAGC CGCAAATAGA AGCAATTCGC ACAGCCATCG CAGTTTATCG CGACTGGATG 
ACAGGCGAGA CGCCTCCACC GTTTCTATTG GAGCCAAACG ATGATCCACC GCCTCCATCG 
AATGCGGGTG GAACTCCCAG GAGTCAACGA CTTCGCACAC CATCTTATGT GGGCGCTATT 
GCAGGCTCCA AAGATCAGTT GGTAGTTAGA GCTGGCCGAC AAAATGTGTT ACAGGTCTTT 
GTGACCAATG CGGCAAACGT GTTCCTGGTG AACACGGCCA ACCTGAACAT CTGCTTTCCC 
ACCCGCTCGC GAAGTTATCG CTCCACGCCA CTGGAAGAGC AGACGGAGAT CTGCAAGAAG 
GTGCTGAATG TGTACCGCAC GATGGTTATG AACACGGAAA TGGACACGCG CACCTGGGAG 
CAACTGCTTA TGGTTTTACT GCAGGTGACC TCAATTGTGC TACACCAAAA TCAGCATACA 
TTGCCAAGTG GCACCAACAA GAGTGCCACT TTGGGCGGGA TCCTAGGGTC AGCTATATTC 
CAAACTCTCA TTGTCACATG GATACGAGCG CACACCAAAG TGCCTGTCAA CGTGCTGCTG 
TGGGAAAAGT TTTTAAATGT CCTGCAATCT TTGACGCACC GCGAGGAGCT CATTATTGAA 
TGGAATAAGA CGATTCAAAC TCTGACGCGT GTGTTCTCGC GATACACTTA TGGCATAAAC 
TTGCTGGATC TACCTTTGGA TCGAGTGGCT GAGAGTCGCG CGGAAAAGAG ACGTCGCATT 
GGCAGCGTCT GGCAGGGATC GGGATCAAAC AGCGCTGCTA ATGGAGGAAG TGCTGCTTCT 
GCTGCAATTA GCCAGAACAG ACAGCGGGAA TCTCTGGCGG AGTCGAGTAG CAGTCGCTCG 
GAGGAGACCT CGTCGCAGGT GCCTCTTCCA AATCACCCTC GACCTCATCA CCAGCACACG 
CAATCCCAGG GAGGCACTGG CCATGGAACA CGACCGATAC CTTTAACTCC CATGCTAAGT 
AGAAGCTACA GCGAAGGAAG CCTGGCTTCA GCGGCTCGTA GTTCGAGGAT ACGACGTCGA 
CGGGCGGCCA CTCCCAAGCC CAAGGCGCTA CAGCCGTCAC AACTCGCAGA GAACCGCATT 
AATCCGCAGG ACTTGCGGCG CGCCATGTCC CTTGATTCGT TGGCGCGGAA AGGTGATGCA 
GAGGAAACCG ATAGCTACCA GGAAGGAGAC AATGAAAGTG GAGCTGGCTC GAGGAGTCCG 
TCACCCACAG CCAGCAGTGG AATTGAAGGT GGCTCCATCA AGGATGCCCA ACTGCAGATT 
GATGCGAGCT TGGATGATGC AAATTCTGGT AGTTACGGAA GTGGCAGTAA CTCGGGCAGT 
ATTTCCGGAC GTCGCTGTAT TATTCTAGGA GGCTCAGCAG AGGGTTGGCA TCCAGATTCC 
ACTTCCATTA TGTGGAAACG CATGCTTGGC GCTCTAGGAG ATGTTAATCG CATTCCCAAA 
GCTGATTTAC ACGCCCAGGT GTTTATGCAT CTGCTAGAAA TGACGCAAAA TCTCATCAAG 
ATCAAGCAGA ACCAAGGAAT ATCCACGGAT AATCAGAACA CGCAACCGAT GCCACCTTTG 
GTACCACCCA TTGGAATCGT GGCACCATGG TGTTACGGAT CCCTTTCTCT GGATCGCTCT 
TTCAAGAAGG GCAAACTCTG GGCCCTTCAA TTGCTCTGTT CGCTTGCGAT TCAAGGTGCT 
GTCAACATGC AACAGCTGCC CTTGTTCTAT CATGCTCTCC ATCAGCTGCT GACTGGCGAG 
GATCGCGACT TGATTTATGC AATTCTTAAG CACCTAGAAG GGCCTAGGCT CCTAAGTTTG 
CTTTTGCCAG GACACACCCT GCTGCTGTTG GACTTGGTCC ATGCAAGTGC TATACTGCTG 
ACTTCTCTGG AGGTCTCTAG GAGCACACCA AGAGCGGAAG TTGCTGCTCT TCTGGGATCA 
CTGCTATGTT ATCCGTCCTC GTTGCTGACA CGTTCCGTTC TGCAGCCCAC GCCGCAGAAG 
TTTGAGCTTA TGGAATGTTC GGACTTGCAA GATCACATCC TGAACATTGT GCTGCGATGC 
GCCAGGCGAG AGCCTTCAGC CAAGGCACGA TGCATTGCAC TCTCTCAGCT CGGACAGTGG 
TTGCTAATGC GCCTGTCTCA GCCACTGCCG GCTTCGAATT CCGGAAGGGC TAACCTCTTT 
CAACAAGCGG TGCCGCATCA CAAGGATGTG CATCCTAAGG CGAGCAGTGT CTACAATCCG 
CGCATAAAGG AGGTGCTACA AGTGCTGCTC CAGGCGTTGC AGTTTAAGCA TCACACCATT 
GCCATTGTAG CGGTGGATTC CTTAAAGTTG TGTGCCGAGC GTGGACGCCA ACTGGCGGCT 
ATTGAAAGAG TTCCCCAGCT AATCATCACT GCCATCTGCA AAGCTTTGGA AATTCAAAGC 
GTAACCAAGC CTAAGGATTC CGACAAAGTG GTGCTGACTT CTCTGATGTT GTGCCTGGGC 
GAATTCTGTA TGGCTATTCC GGCTCCTATT ATGCTGACGC CCTTCAACGA ACAAGGTGAT 
ACGCTAGTGC TCCAGGTGCT CAGGGTACTG TTGCAAGTTG CTTCCGGTGC TCCGCGTCAT 
GAGAGAGTGA AACTGACGGC CGATGATGAC TTTGATATGC ACATCGCACA CGACGATCTT 
CAAGGCGATG GACGCCTGCC GGAAGCCACC TATCAGACCT CCGAGACCAT CCAGAAGTGC 
ATTACAGCCA TTAAGCTGTG TGCCAAAGCA GTGTCCATGC ATTTGGTCAC CCACATCGGT 
CACTTTCCGA TGGGAATCGG AGCATCTCGT TTGAGTTCCA TGGTCGAGGA GCAGGATGAC 
ATTGGAAATG CCGCCTATGG TGGTCAGGTG GAGACGAGGC GCGATTCTGT GGAGCTTCCT 
TCCGTTGTAA GTGCCCAAAA TATGCAGCTT TTCATGCTAA ATTCTGGTCT GGTAGCCAGT 
TTTATTGAGC TGCCAACATT AAAACTACCC GGTGGAGGTA TAACCGCTGG CTTGGTTACA 
GCCGATAAGC AAGTTCGGGT CTTAATGAGG GATCTCAATG GAAAAGCTTG CTGGGACGCT 
TCTATCTTGT ATTCAGAACC GCGCAATGCG GAGGAGCCTC CAAAAACCAC ACCAAAGATT 
CAACATAGCC AACCGTTGGA TTCCATGGCG ACCTCCATGG TGACACACAC ACCAAGTCCT 
CGACACACTT TGCGACACCG ACCGGCGGGA GTGCTGCCAT TGGCCAAGGA CATGGCTCCG 
GATCTGGATC AATTGGACGA TATGTTGGCC TATATTGGAC ACACCAGTCC AGAGTGTGTG 
GCACCCACTG TAAGCCAGCT GAATGCTCCA ACGGCCAGTC CTCTGAGTGG AAACCAAGAA 
GCTCAGGCCA TTTCGGTAAT TCTGAATCAA CGCCTTTTGG AGCAGGAGTT TGTGACCCAC 
TCGACTCAGG CTCCATCGCC GGCATTGCGA CACGCCAGCT CGAATTCCTC ACTGCAGCAA 
CCGGATCAGA GGTCACTGCA CTCCACAACA GCCTCCTTTG ATTCCTTACC AACCCGCACA 
GAGATGCCGT TCCAATATTG CCGGCTGCTC TTTTCACATT TGGGACTAGC TGGCTGGGAG 
CGACGGTCTA GGACGCACTT GCTTCAGAGG AGCGAAAAAC TCATGAGAGA ACTGCGGAAC 
GTTGATCTCC AAAAATGCCG GGAAACCCAC AAAATGGCGG TTATTTATGT GGCCGCCGGG 
CAGGAAGATA AGGGAAGCAT CCTTAGGAAT ACGAGTGGAA GCAGCACCTA CGAGATGTTC 
GTTTCTGCCT TGGGTTGGGA AATCGATCTG GAGACACACA ACGGCTTCTT GGGCGGATTA 
CCACGCCAAG GATGCGGAGC TACAGCTCCC TATTACGCTA CCCCTTTTCT CGAGGTGGTT 
TACCATGTGG CCACCAGGAT GCCCTCGGAT TCTTCGGAGG CCATGCTACT GAAGACGCGG 
CATCTTGGCA ACGATGAGGT TCACATTGTG TGGAGCGAAC ACAATCGGGA TTACAGGCGG 
GACATATTGC CCACTGAGTT TTGCGATGTG CTGATTGTGG TTTATCCGCT GCGGAATGGT 
TTGTTCCGGG TGACTGTGAA CAGGAAGCCT GAAGTTCCAT GGTTTGGACC ATTGGCCAAC 
GAGAGCGTGG TAAGTGGCGC CTGCTTGGCC ACTCTTATTC GGGCAACCGC AATCAATGCC 
AGTCGAACGA AGCGCGCTGC CCTGCCGCTC TACCAACAAT TCTATGAGGA GCGCAACCGG 
TCGTTGGATA GCGTATCATC TCGGTACAAG GAGAGCACCA CTTTCGAAGA CTTTGCCAGC 
CGCATCTACA ATCCCATGCC GCTGTCTACG CTGGGCACAC TAAGGGAATC CAATGCCAGC 
AGCTCGGCCG CACCTCTGGC TTCTGCATTG CTGGATCACA ATCGTGCCTC CGTCAAAGGT 
TGGGTACAAG CCTCGATTGA TTCGGGGCCC ATAATGGGGA TTGCGCCATC GGCTTCAGCT 
GGATCCACCG CGGCCATGGA GGCAGCCACC GGAATGTCCT CCGCTTCGCC GCGTGGCCCT 
CGAAAACTGG GGGCTCCGTT CAAGAGCGTG ACCAAAAAGC ATTCGCTGCA GCATATCGCC 
GTGGGAGGCG GCGCAGGAGC TGGCGGTGAC ACGCCGCCCG AGAGCCCAAC ATTGCCGCAG 
CGACGCTTCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5521 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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