Ralgapa1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ralgapa1
Entrez Gene ID	56785
Full Name	Ral GTPase activating protein catalytic alpha subunit 1
Synonyms	GRIPE,Garnl1,Tulip1,p240
General protein information	Preferred Names Ral GTPase activating protein catalytic alpha subunit 1 Names ral GTPase-activating protein subunit alpha-1 GAP-related-interacting partner to E12 GTPase activating RANGAP domain-like 1 GTPase activating Rap/RanGAP domain-like 1 GTPase-activating RapGAP domain-like 1 Ral GTPase activating protein, alpha subunit 1 (catalytic) tuberin-like protein 1 tulip 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q23
Summary	mouse homolog interacts with the HLH region of E12 and may be involved in neuronal differentiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_020083.3	NP_064468.3	ral GTPase-activating protein subunit alpha-1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ralgapa1	NP_001003719.1
Homo sapiens (human)	RALGAPA1	NP_001269972.1
Pan troglodytes (chimpanzee)	RALGAPA1	XP_003314394.1
	RALGAPA1	XP_001088633.1
Rattus norvegicus (Norway rat)	Ralgapa1	NP_064468.2
Drosophila melanogaster (fruit fly)	CG5521	NP_001163748.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004143	XP_313024.5
Bos taurus (cattle)	RALGAPA1	XP_005222134.1
Gallus gallus (chicken)	RALGAPA1	NP_001264376.1
Danio rerio (zebrafish)	ralgapa1	NP_001139776.1

Gene Ontology
Bibliography

Related articles in PubMed

Tuberous sclerosis tumor suppressor complex-like complexes act as GTPase-activating proteins for Ral GTPases.
Shirakawa R, Fukai S, Kawato M, Higashi T, Kondo H, Ikeda T, Nakayama E, Okawa K, Nureki O, Kimura T, Kita T, Horiuchi H
The Journal of biological chemistry284(32)21580-8(2009 Aug)

Cloning and characterization of GRIPE, a novel interacting partner of the transcription factor E12 in developing mouse forebrain.
Heng JI, Tan SS
The Journal of biological chemistry277(45)43152-9(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ralgapa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ralgapa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa43448	NM_020083.3 Latest version!	Rattus norvegicus Ral GTPase activating protein catalytic subunit alpha 1 (Ralgapa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ralgapa1 ( NM_020083.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_064468.3 Rattus norvegicus Ral GTPase activating protein catalytic subunit alpha 1 (Ralgapa1), mRNA.

Price & Availability↑

CloneID	ORa43448
Clone ID Related Accession (Same CDS sequence)	NM_020083.3
Accession Version	NM_020083.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	ral GTPase-activating protein subunit alpha-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01044253.1, AAHX01044250.1, AAHX01044249.1, AAHX01044248.1, AAHX01044247.1, AAHX01044245.1, AF041107.2, AAHX01044244.1, AAHX01044243.1, AAHX01044242.1, AAHX01044241.1, AAHX01044240.1 and FQ214235.1. On Dec 9, 2014 this sequence version replaced NM_020083.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to correct errors in the reference genome assembly. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGACTCT 
ATTGACCTTA AACAGTTCTT TGACCAGCAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC CAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA 
TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG 
GATAACTGTA GCAAAGAGCA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG AATATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TTCCCCCGCA GTCAGGAGAC 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACCTCTG CAAGGAGAAT 
AGTTTGTATC ATCCTGTACT TGATATCCCA CAGGTCAGAC CAAAGCCTCA TTATGTGATG 
GTAAAGAAGG ATGCTGAAAC CAATGAAACG ATATACTGTA CAAAAGAGCC TTTCATCCAG 
GCTCGGGTCA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCGCATTCA 
GGACCTAATA TTCCTGGGAT GGAGGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TCTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGC AGACAAAACC 
GCAGAGCCCG AGCAGTCGCA TTCCAATACA AGCACTCTCA CAGAGCGAGA GCCCAGCTCT 
TCCAGTCTCT GTAGCATCGA TGAAGAACAC CTCACGGACA TTGAAATCGT TCGCAGGGTA 
TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAGTGGATT 
CAACAGGAGG GAAAACCTTT GTTTATGCAA GAGCCTGAAG AAACTGTGAT CACTTCTTCA 
GATATACCTT GCAGTGAAAA TGTCACAGAC CATGACATTT CAATAGAAGA CGGAGAGAAG 
AGAGAAGAGG AAAATGGGAC CAATATTTCT GAACATGTTC GAAATTCTAC CTGGACAAAG 
AATGGTTCCT ACCAGGAAGC TTTTCATGTC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACTC AGGCTGTTTT ACAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCAA ATGAAATAAA AAATCTTCTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATAT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTACTCG TGTTGCTCAG AGTCACGGAG TCTGTACTGA AGATGTCGTC ACAAGCTTTT 
CTGCAGTTCC AGGGGAAAAA AAACATGACC CTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCGCTGA CCATGGAGAC ACTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGCGATCAGC CTGGCCAGGC ACCCATGAGA CAGAGAAGTG CAACGACTAC TGGCTCCCCG 
GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATTCTCAA 
ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGTAATGAA 
GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG 
CCTCCTCTTA ATAGTGAGAC TGGCGGCAGC AGCGCTAATG TTCCTGACCT GATGGATGAG 
TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGAAGTAGT 
CCAGGCAGCC TTGAAATTCC GAAAGACCTT CCTGATATTC TAAACAAGCA GAATCAGATG 
CGCCCTGTTG ATGACCCTGG TGTGCCCTCA GAATGGACGT CTCCTGCCAG TGCAGGGAGC 
AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGC 
CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG 
GATTCAGGCT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCTAG CCTAACGACG 
CTTCATTTAG ACTCAGAGAC AAGCAGCCTT AATCAACAAG CATTCTCTGC TGAAGTTGCA 
ACTGTCACTG GTTCAGAAAG TGCTTCCCCT GTTCATTCAG CTCTGGGGTC CAGGTCACAG 
ACACCTTCCC CTTCTACGCT GAATATAGAT CACATGGAAC AGAAGGATCT ACAGCTTGAT 
GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCAGATGACC TAGAAATCAG TGAATTTCCT 
TCTGAATGTT GTAGTGTGAT GGCAGGAGGT ACGCTCACTG GGTGGCATGC TGATGTTGCT 
ACTGTTATGT GGCGAAGAAT GCTTGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT 
GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC 
AGAGATAATC TTGGCATTTC AGCCGATAAC CTGACTTCAC CTTCTCCACC TGTTTTGATT 
CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT 
AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT 
GTATCCCCAA ATAGAGATTT TTTAACGCAT TTCTACAATA TAATGCATTG TGGATTGCTT 
CATATTGACC AGGACATCGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA 
CTTGGTTTGC CTGGGGCCAC AATGCTTATT ATGGATTTTA TTATAGCGGC TGGGAGAGTG 
GCTTCTTCAG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AGGTTCTTCT GGGATCTTTG 
GTTTGCTTCC CCAACTTATA CTGTGAGCTG CCTGCTCTGC ATCCAAGCAC GCCTGACATC 
GCTGTGTCTC AGTTCACAGA CGTCAAGGAA CTTATAATTA AAACTGTCTT AAGCTCGGCA 
AGAGACGAAC CCTCTGGCCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATATGGATT 
TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT 
TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCATG TAGCTTGTAA CATGCTTCAC 
ATGCTGGTTC ATTATGCACC TAGACTTCAG ACTTACCAGC CTGACTCTCC CTTGAAAATT 
ATTCAGATCC TAATAGCAAC TATCACACAC CTTTTGCCAA GTACAGAAGC TTCATCATAT 
GAAATGGACA AGAGGTTGGT TGTGTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC 
TTACCTTTAA AGACAATACT CCAATCAGTC CATTCTACAG GAGCAGAAAA TGAGAAGACA 
GAAAAATCTG TCCTCAACTG TATTTATAAG GTTTTACATG GGTGTGTATA TGGAGCACAA 
AGTTTTAGCC ATCCAAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT 
GACCCTTTTA TGCATTTGGA AAGTCTGAGA GAGCCTGAAC CTCTACACTC TCCTGATTCA 
GAACGGTCTT CTAAACTGCA GCCGGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA 
ATTTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACCTGGG CCATTATCCA 
ATGAGTGGTG GTCCTGCTAT GCTAACGAGT CAGGTGTGTG AGAATCATGA CAATCATTAT 
AGCGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGACAGTC CAAATATTCA GTTCTTTGTG 
CTGAATAATA CAACCTTAGT GTCTTGTATC CAGATAAGAT CAGAAGAGAG TGTGCCTGGA 
GGAGGTTTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT 
CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GGCCACCCAT TGTAAGTGGT 
TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTTACC AAGAGAAACC AGAAGAGCCT 
CCTACATCTA ATGAATGCTT AGAAGACATA ACAGTAAAAG ATGGACTTTC TCTCCAGCTT 
AGAAGATTTA GAGAAACTGT TCCAACTTGG TCTACAATAA GAGAGGAGGA AGATGTTCTT 
GATGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAAT GTTTACAGAG AACTGGAATC 
TCCCTCAATG TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTC 
ATTAATGCTA TCCTTAAGCA ATACACAGAA GAAAAAGAGT TTGTAGAAAA GCACTTTAAT 
GACTTAAACA TGAAAGCCTC AGAACAAGAT GAACCAACAC CTCAGAAACC TCAGTCAGCA 
TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTGCTCAGAG AACTGAGGAA CTTGGATTCA 
AGGCAGTGCC GAGAAACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT 
AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTTGCTGGC 
CTTGGTTGGG AGGTAAACCT TACAAATCAC TGTGGCTTCA TGGGAGGACT GCAGAAGAAC 
AAAAGCACTG GACTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAAGT AATATTTCAT 
GTATCTACAA GAATGCCTTC TGAATCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA 
GGAAATGATG AAGTACACAT TGTTTGGTCT GAACATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AGTTTGGTGA TGTCCTCATT GTAATATATC CAATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAAAA GCCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT 
ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT 
GCTCTGAAAT CACTGATTCC ATTGTATCAA AACTTCTACG AGGAGAGAGC ACGGTACCTG 
CAGACAATTG TCCAGCACCA CTTAGAGCCA ACGACATTTG AAGACTTTGC AGCCCAGGTT 
TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGATGCAG ATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064468.3
CDS	12..6119
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENADSIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEVTPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNLCKENSLYHPVLDIPQVRPKPHYVMVKKDAETNETIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESKNDTVDKADKTAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEGKPLFMQEPEETVITSSDIPCSENVTDHDISIEDGEKREEENGTNISEHVRNSTWTKNGSYQEAFHVSEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDSQILPRSTRVRHFSQSEDTGNEVFGALHEEQPLPRSSSTSDILEPFTVERAKVNKEDTSPKLPPLNSETGGSSANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCEGRKFDNFGFGTDIGIPSSADVDSGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSTPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYAPRLQTYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTILQSVHSTGAENEKTEKSVLNCIYKVLHGCVYGAQSFSHPKYFPISLSDLASVDYDPFMHLESLREPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFDSPNIQFFVLNNTTLVSCIQIRSEESVPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVSGLPEPTSFILSMSYQEKPEEPPTSNECLEDITVKDGLSLQLRRFRETVPTWSTIREEEDVLDELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSESDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHFPADADH

Target ORF information:

RefSeq Version	NM_020083.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Ral GTPase activating protein catalytic subunit alpha 1 (Ralgapa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020083.3

ORF Insert Sequence:

ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGACTCT 
ATTGACCTTA AACAGTTCTT TGACCAGCAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC CAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA 
TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG 
GATAACTGTA GCAAAGAGCA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG AATATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TTCCCCCGCA GTCAGGAGAC 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACCTCTG CAAGGAGAAT 
AGTTTGTATC ATCCTGTACT TGATATCCCA CAGGTCAGAC CAAAGCCTCA TTATGTGATG 
GTAAAGAAGG ATGCTGAAAC CAATGAAACG ATATACTGTA CAAAAGAGCC TTTCATCCAG 
GCTCGGGTCA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCGCATTCA 
GGACCTAATA TTCCTGGGAT GGAGGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TCTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGC AGACAAAACC 
GCAGAGCCCG AGCAGTCGCA TTCCAATACA AGCACTCTCA CAGAGCGAGA GCCCAGCTCT 
TCCAGTCTCT GTAGCATCGA TGAAGAACAC CTCACGGACA TTGAAATCGT TCGCAGGGTA 
TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAGTGGATT 
CAACAGGAGG GAAAACCTTT GTTTATGCAA GAGCCTGAAG AAACTGTGAT CACTTCTTCA 
GATATACCTT GCAGTGAAAA TGTCACAGAC CATGACATTT CAATAGAAGA CGGAGAGAAG 
AGAGAAGAGG AAAATGGGAC CAATATTTCT GAACATGTTC GAAATTCTAC CTGGACAAAG 
AATGGTTCCT ACCAGGAAGC TTTTCATGTC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACTC AGGCTGTTTT ACAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCAA ATGAAATAAA AAATCTTCTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATAT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTACTCG TGTTGCTCAG AGTCACGGAG TCTGTACTGA AGATGTCGTC ACAAGCTTTT 
CTGCAGTTCC AGGGGAAAAA AAACATGACC CTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCGCTGA CCATGGAGAC ACTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGCGATCAGC CTGGCCAGGC ACCCATGAGA CAGAGAAGTG CAACGACTAC TGGCTCCCCG 
GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATTCTCAA 
ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGTAATGAA 
GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC 
TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG 
CCTCCTCTTA ATAGTGAGAC TGGCGGCAGC AGCGCTAATG TTCCTGACCT GATGGATGAG 
TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGAAGTAGT 
CCAGGCAGCC TTGAAATTCC GAAAGACCTT CCTGATATTC TAAACAAGCA GAATCAGATG 
CGCCCTGTTG ATGACCCTGG TGTGCCCTCA GAATGGACGT CTCCTGCCAG TGCAGGGAGC 
AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGC 
CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG 
GATTCAGGCT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCTAG CCTAACGACG 
CTTCATTTAG ACTCAGAGAC AAGCAGCCTT AATCAACAAG CATTCTCTGC TGAAGTTGCA 
ACTGTCACTG GTTCAGAAAG TGCTTCCCCT GTTCATTCAG CTCTGGGGTC CAGGTCACAG 
ACACCTTCCC CTTCTACGCT GAATATAGAT CACATGGAAC AGAAGGATCT ACAGCTTGAT 
GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCAGATGACC TAGAAATCAG TGAATTTCCT 
TCTGAATGTT GTAGTGTGAT GGCAGGAGGT ACGCTCACTG GGTGGCATGC TGATGTTGCT 
ACTGTTATGT GGCGAAGAAT GCTTGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT 
GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC 
AGAGATAATC TTGGCATTTC AGCCGATAAC CTGACTTCAC CTTCTCCACC TGTTTTGATT 
CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT 
AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT 
GTATCCCCAA ATAGAGATTT TTTAACGCAT TTCTACAATA TAATGCATTG TGGATTGCTT 
CATATTGACC AGGACATCGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA 
CTTGGTTTGC CTGGGGCCAC AATGCTTATT ATGGATTTTA TTATAGCGGC TGGGAGAGTG 
GCTTCTTCAG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AGGTTCTTCT GGGATCTTTG 
GTTTGCTTCC CCAACTTATA CTGTGAGCTG CCTGCTCTGC ATCCAAGCAC GCCTGACATC 
GCTGTGTCTC AGTTCACAGA CGTCAAGGAA CTTATAATTA AAACTGTCTT AAGCTCGGCA 
AGAGACGAAC CCTCTGGCCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATATGGATT 
TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT 
TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCATG TAGCTTGTAA CATGCTTCAC 
ATGCTGGTTC ATTATGCACC TAGACTTCAG ACTTACCAGC CTGACTCTCC CTTGAAAATT 
ATTCAGATCC TAATAGCAAC TATCACACAC CTTTTGCCAA GTACAGAAGC TTCATCATAT 
GAAATGGACA AGAGGTTGGT TGTGTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC 
TTACCTTTAA AGACAATACT CCAATCAGTC CATTCTACAG GAGCAGAAAA TGAGAAGACA 
GAAAAATCTG TCCTCAACTG TATTTATAAG GTTTTACATG GGTGTGTATA TGGAGCACAA 
AGTTTTAGCC ATCCAAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT 
GACCCTTTTA TGCATTTGGA AAGTCTGAGA GAGCCTGAAC CTCTACACTC TCCTGATTCA 
GAACGGTCTT CTAAACTGCA GCCGGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA 
ATTTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACCTGGG CCATTATCCA 
ATGAGTGGTG GTCCTGCTAT GCTAACGAGT CAGGTGTGTG AGAATCATGA CAATCATTAT 
AGCGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGACAGTC CAAATATTCA GTTCTTTGTG 
CTGAATAATA CAACCTTAGT GTCTTGTATC CAGATAAGAT CAGAAGAGAG TGTGCCTGGA 
GGAGGTTTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT 
CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GGCCACCCAT TGTAAGTGGT 
TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTTACC AAGAGAAACC AGAAGAGCCT 
CCTACATCTA ATGAATGCTT AGAAGACATA ACAGTAAAAG ATGGACTTTC TCTCCAGCTT 
AGAAGATTTA GAGAAACTGT TCCAACTTGG TCTACAATAA GAGAGGAGGA AGATGTTCTT 
GATGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAAT GTTTACAGAG AACTGGAATC 
TCCCTCAATG TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTC 
ATTAATGCTA TCCTTAAGCA ATACACAGAA GAAAAAGAGT TTGTAGAAAA GCACTTTAAT 
GACTTAAACA TGAAAGCCTC AGAACAAGAT GAACCAACAC CTCAGAAACC TCAGTCAGCA 
TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTGCTCAGAG AACTGAGGAA CTTGGATTCA 
AGGCAGTGCC GAGAAACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT 
AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTTGCTGGC 
CTTGGTTGGG AGGTAAACCT TACAAATCAC TGTGGCTTCA TGGGAGGACT GCAGAAGAAC 
AAAAGCACTG GACTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAAGT AATATTTCAT 
GTATCTACAA GAATGCCTTC TGAATCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA 
GGAAATGATG AAGTACACAT TGTTTGGTCT GAACATACTA GAGACTACAG GAGAGGAATT 
ATTCCTACAG AGTTTGGTGA TGTCCTCATT GTAATATATC CAATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAAAA GCCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT 
ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT 
GCTCTGAAAT CACTGATTCC ATTGTATCAA AACTTCTACG AGGAGAGAGC ACGGTACCTG 
CAGACAATTG TCCAGCACCA CTTAGAGCCA ACGACATTTG AAGACTTTGC AGCCCAGGTT 
TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGATGCAG ATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tuberous sclerosis tumor suppressor complex-like complexes act as GTPase-activating proteins for Ral GTPases.
The Journal of biological chemistry284(32)21580-8(2009 Aug)
Shirakawa R,Fukai S,Kawato M,Higashi T,Kondo H,Ikeda T,Nakayama E,Okawa K,Nureki O,Kimura T,Kita T,Horiuchi H

Cloning and characterization of GRIPE, a novel interacting partner of the transcription factor E12 in developing mouse forebrain.
The Journal of biological chemistry277(45)43152-9(2002 Nov)
Heng JI,Tan SS

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Ralgapa1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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