ralgapa1 cDNA ORF clone, Danio rerio(zebrafish)

The following ralgapa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ralgapa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa149647 XM_021467309.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa149648 XM_021467310.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa149649 XM_021467311.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa149650 XM_021467312.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa149651 XM_021467313.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa149652 XM_021467314.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa149653 XM_021467315.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa149654 XM_021467316.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa149655 XM_021467317.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa149656 XM_021467318.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa149647
    Clone ID Related Accession (Same CDS sequence) XM_021467309.1
    Accession Version XM_021467309.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGGTG CACTTCCTGT CTCTGACAGT CCCTCTCACT CCCCCCACTT TACAGCCACC
    ACCACCACCA TTGAGTTCCC CCACAGTTCC CCAAACCCAC AGCAGCTCAC TGACCCACAG
    CAGCTCCCCG ACAGCCAGGC GTCCCTGGAG GTGGCCGAAA GTATCTATGA CCATCTCTGC
    CAAATGCCAG GCCAGCAGCC CCCCTTTATC CCCGATTGGT CCTGTCCAGC CGCGGCCTCG
    GCCAATCAAC CCGAGGGCGA AGCGGAGGAG GACGTCTTCA GTGCTTTTCG GGAGTTTTGT
    CACAGGGAGA ATGTGAAAAT GGAAGCAGGT GCAGATTTAA AGCAGGTGCA GTTTGAGGGC
    GATTGGGCTC TAGAATGGGA TAGAGAGATG GAGCAGAGCG AGAAGAGTTT GTATGAGTGC
    TTAGAGGAGT GTGATTGGGA TTTGAGTGTT AGTCCGCAGC CAGCAGAGGA CGGTAAAGGA
    GCACAGCTTC AGCGGCAGGA CGCCACCGAC AGGGCTAGCG AGAGTGAGGT TAGTCCACAG
    GCTTCGCATG GACCCATGTT GTCAAATATT GATCACAAGA AACGCCACAG CAGTGCGGTC
    CATGTCAGTT TCCGGCCCTC CACAGAGTCT GTGCTGTTCC ACAACCCTTT GGACCCCAAG
    GAGGCACACT GGAAAACCCG GCTTCGCCGT CTCGGGCACT TCAGCAGCCA CTCGGTTGGG
    GCGAAAGGTG TAGGGGAGGG GGTCACCTGT TGTGAAGTGG TGGAGCGGGA AAGAGGCTCC
    ATCAGCGGGG CTAGACGTGG ACGACTTGGT CGTCCGACTC TTCGGCCTCG AGGGTCCCGA
    TCTAGATCTC AGGAGTCAGG GTGCAGCCCC TCACGACAGC AGCAGGTGGC TCTGTTGGGT
    GGGGTGTATA AGTCTATGGT CCACGCTCTC TCCAAACCCA AGGGCCAGGC AGCATCGCCC
    CAGCGCCACA GCAAATCGCC CGCCACTGAG GCCCACCTGA AGGACCTGTA TGCTAGCGTT
    ATGGGCTATT TTGGAAGAAA AACATCAGCC AATAAAGACG AGCTGACGCC GAAGATGCGG
    CCCATGTCTA ACGATGCTGG CAGCAGCAAC CCCAATGTCA GTGACCTCAT GGACGAGTTC
    ATCCAGGAGA GACTGAGAGC CAGAGGCAAT TCTGGCATGA CGAGGCGCGG CAGTAGCCCA
    GGAAGTCTGG AGATCCCTAA AGATCTGCCA GAGCTGCTGA ACCGTCAGAA CGCCACACGT
    CCCGCAGACG ACCCCGGTGT GCCCTCTGAG TGGACCTCGC CTGCCAGTGC CAGTGGAAGT
    GACCTCATAA GCTCTGACAG CCAATCGGAT TCCTTTAACG CTTTTCAATA TGACAGCAAA
    TTCGAAAATT TCAGCTTCCC CCCTGAGACG TGTGCTTTGG CGTCTGCAGA TCAGGATAGT
    TTGAGTGGGA CAGGACAGAC AGCGGAGGAA CAGGAGCTTT CCAGTCTCAC AACACTTCAC
    ATCGACTCTG AGACCAGCAG CCTTAGTCAG CACGGCCTGT CTGCTGATAC TGTCACTATT
    ACTGGCTCTG AGAGCGCATC TCCAATGCAC TCTCTAGGAG GATCTCGATC GCAGACGCCA
    TCTCCTGCAA CCCTGACCGC TGAACACGTC CAGCATAAAG AGCTGCAGCT GGATGAGAAG
    ATGCACCATT CTGTTCTGCA GACCCCTGAT GACCTTGAAA CCAGCGAGTT TCCCACTGAG
    GACTGTAGTG TAATGGCTGG TGGCACTCTG ACTGGCTGGC ATGCTGATGT CGCAACAGTA
    ATGTGGAGGA GAATGCTTGG GATTCTGGGA GATGTCAACT ATATAAAGGA CCCTGAGATC
    CATGCGCAGG TTTTTGACTA CCTCTGCGAA CTCTGGCAGA ACCTGGCCAA GATCAGAGAC
    AATCTCGGGA TCTCCCTTGA CAACCAGTCG TCTCCGCCAC CACCAGATCT GATTCCACCT
    CTGCGGATTC TGACTCCATG GCTTTTCAAA GCGACTATGT TGGGCGAACG CTACAAGCAG
    GGAAAACTGC ACGCCTACAA GCTCATCTGT AAGATCATGA AGAGAAGACA GGACGTTTCC
    CCAAACTCTG ACTTCCTCAC GCACTTCTAC AACATCATGC ACTGCGGCCT GCTCCACCAA
    GACCAGGATA TTGTGAACAC CATCATTAAG CACTGCTCTC CACGCTTCTT CTCTATTGGA
    CTTCCAGGAG CCACAATGCT CATCCTGGAC TTCATCGTGG CAGCTAGTCG TGTCACTGCC
    TGCTCGTCCC TCAATGCTCC ACGGGTGGAG GCTCAGATCT TGTTGGGTTC CCTGGTTTGC
    CTCCCAAACT TGTACAATGA ACTTCCTGCA CTGCATCCTA CAACAGCCGA CATCATCATG
    ACCAAATTCA CTGATGTCAA GGAGCATATA ATCAAGCACA TCCTGACCTC TGCCAGAGAT
    GAGCCTTCTG CTCCAGCCAG GTGTGTGGCG CTCTGTAGTC TGGGTATTTG GCTGTGTGAA
    GAGCTGGTTC ATGGCACTCA GCATCCGCAG ATCAAAGAAG CGCTTAACGT CATCTGTGTC
    ACTCTCAAGT TCTCCAATAA AAACGTCGCC CTGGTGGCTT CAGACATTTT GCACCTGCTG
    ATAAACTACG TGGATGAACT GGAGAAATTT CCACCCAACA CTCCAAAGAA AATAGTGGAG
    GTTCTTATTG CAACCATCAC TTACCTTCTT CCAGCCACAG AGTCCTCTCC TCATGAGCTG
    GACAAGAGGC TGGTTGTTTC TCTACTGCTG TGCTTGCTGG ACTGGGTGAT GGCTCTACCT
    CCGAAAACAC TCCTCCAGCC GGTCCAAGGA GCCTCGGATA AGGAGAAAAC TGACAAGTCT
    GTCCTCAGCT GCATCTATAA AGTCCTCCAT GGTTGTGTCT ATGGGGCTCA GAGCTTCAGT
    AACCCCAGTT ACTTCCCCAT TCACCTGTCA GACTTAAGCA GCCCAGACTA CGACCCCTTC
    TTACTACTAG AAAACCTTAA GGAGCCGGAG CCACTCCATT CACCTGACTC TGAACGCTCC
    TCCAAACTGC AACCAGTAAC TGAAGTGAGG AGCCGCATTC AGCAAGGCCT TGTTTCAGTT
    GCGGCACGCA CTGTCATATC TCACCTAGTC AACCATCTGG GTCACTATCC CATGAGTGGT
    GGTCCGGCCA CTCTGACGAG CCAGGTGTGC GAGAACCAGG ACAACCCATA CAGTGAGAGC
    GCAGACCTGA CCCCTGAACT CTTTGACGCC CCCAATTTGC AGTTCTTCGT GCTTAATGGC
    ACCACCCTGC TGTCCTACTT ACAAATTCGG GCTGAAGATG GCCTGCCCGG TGGTGGGATG
    TCAGCTGGCC TCACCACCAC CAATGCTTGT GTTCGGGTGA TTGTGAGGGA CATTTCTGGT
    AAACACTCCT GGGACTCAGC TGTTCTGTAT GGTCCACCTC ATTGCATCAC TCCTAGTCAA
    ACTTTGCACC TTTACACGTC CTCTAATCCG AGAGGGGAAC ATAATAGAGA TGAGGACAGT
    TTAGAGAGGG GCAGCCAAGA AGAACCAGAG GATGGAGCTT TTCAGGAAAG CGAGGAGGAC
    TTGGATGAGC ATGAGGAGGC GGAGGTGAAA GAAGACGAGG TGGAGGAGGA AGAACTTGAG
    GAAGAGATTG AGGAGGAAGA GGTTGCACTG GAACTTATGG CCCCGCAGGC CAAACGGCGG
    TGCAGAGAGG TCATCCCGAA CTGGGACTCC CTGCAGGATG GGGAAGACTC CCTAGATGAG
    ATGCTGCAAT ATCTGGGATA CTCCAGCCCA GAGTGTCTGC AACGTACTGG TGGACCTCTC
    AACATACCTG CCCCACCTCC AAACTGTGTG TCCGAGAAAC AGGAGAATGA TGTCATCAAT
    GCCATCCTGA AGCAGTGTGC CGAGGAGCAG GACTTCACAA TTCACCGTGG TAATGAGCTG
    AACATGAGGG CCGTCGTTCA GCAGGAGCCA AGCCCACAGA GACCACAGTC TGCCTTCTAC
    TACTGCAAAC TGCTCCTCAA CATACTAGGC ATGAACTCTT GGGAGAAGAG GAACAGTTTC
    CACCTGCTGA AAAAGAATGA AAAACTACTG AGAGAGCTGA AGAATCTGGA CTCCAGGCAG
    TGTCGTGAAA CACACAAAAT CGCGGTGTTT TACGTGGCAG AGGGACAAGA AGACAAGCAC
    TCCATTCTGT CCAACACAGC CGGGAGTCAG GCGTATGAAG ACTTTGTATC AGGCCTGGGC
    TGGGAGGTGA ACCTGACGAG TCACTGTGGC TTTATGGGTG GCCTGCAGCG GAACAAGAGC
    ACGGGTTTTA CCATGCCTTA CTTTGCCACC TCTACAGTGG AAGTGATGTT CCACGTCTCC
    ACACGCATGC CTCCAGACTC AGACGACTCG CTCACTAAAA AGTTGAGACA CCTGGGTAAT
    GATGAAGTGC ACATCGTGTG GTCTGAGCAC TCGCGGGACT ATCGGAGAGG CGTCATTCCT
    ACTGAGTTTG GAGACGTGCT CATCGTCATC TACCCTATGA AGAACCACAT GTACAGCATC
    CAGATCATCA AGAAACCTGA GGTGCCGTTC TTTGGTCCCT TGTTTGACGG AGCCATAGTG
    GATGGGACTA TATTGCCCAC AGTGGTGCGA GCGACAGCCA TCAACGCCAG CAGAGCTCTG
    AAATCCCTCA TCCCTCTATA CCAAAACTTC TATGAAGAGA GGGCCAGATA TCTGGAGACG
    ATAGTCCAAC ATCACTTGGA GCCCACGACA TTTGAGGACT ACGCCGCTCG TGTCTTTTGT
    CCTGCGCCTT TCCACTATCT TCCGTCTGAA GCAGAGAGAA ATGCTGTGAT TTTTCTGCCC
    CTCCCTAAAC CTCCACTAGA AAACATTGGG CTCACCAAGG TAAAGGGCAA AGGCTTGAAA
    CGGAAGGTCT TTCACGCTAG CTGTGAATGG CTCATGCCTC GAGAGTCAGC TGGCAGAAAG
    TCCAGCAATG CAGGTCGACG GGAGCGACTT AGCCTCGCCA ATGTCACCTC GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322984.1
    CDS656..8212
    Translation

    Target ORF information:

    RefSeq Version XM_021467309.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467309.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGGTG CACTTCCTGT CTCTGACAGT CCCTCTCACT CCCCCCACTT TACAGCCACC
    ACCACCACCA TTGAGTTCCC CCACAGTTCC CCAAACCCAC AGCAGCTCAC TGACCCACAG
    CAGCTCCCCG ACAGCCAGGC GTCCCTGGAG GTGGCCGAAA GTATCTATGA CCATCTCTGC
    CAAATGCCAG GCCAGCAGCC CCCCTTTATC CCCGATTGGT CCTGTCCAGC CGCGGCCTCG
    GCCAATCAAC CCGAGGGCGA AGCGGAGGAG GACGTCTTCA GTGCTTTTCG GGAGTTTTGT
    CACAGGGAGA ATGTGAAAAT GGAAGCAGGT GCAGATTTAA AGCAGGTGCA GTTTGAGGGC
    GATTGGGCTC TAGAATGGGA TAGAGAGATG GAGCAGAGCG AGAAGAGTTT GTATGAGTGC
    TTAGAGGAGT GTGATTGGGA TTTGAGTGTT AGTCCGCAGC CAGCAGAGGA CGGTAAAGGA
    GCACAGCTTC AGCGGCAGGA CGCCACCGAC AGGGCTAGCG AGAGTGAGGT TAGTCCACAG
    GCTTCGCATG GACCCATGTT GTCAAATATT GATCACAAGA AACGCCACAG CAGTGCGGTC
    CATGTCAGTT TCCGGCCCTC CACAGAGTCT GTGCTGTTCC ACAACCCTTT GGACCCCAAG
    GAGGCACACT GGAAAACCCG GCTTCGCCGT CTCGGGCACT TCAGCAGCCA CTCGGTTGGG
    GCGAAAGGTG TAGGGGAGGG GGTCACCTGT TGTGAAGTGG TGGAGCGGGA AAGAGGCTCC
    ATCAGCGGGG CTAGACGTGG ACGACTTGGT CGTCCGACTC TTCGGCCTCG AGGGTCCCGA
    TCTAGATCTC AGGAGTCAGG GTGCAGCCCC TCACGACAGC AGCAGGTGGC TCTGTTGGGT
    GGGGTGTATA AGTCTATGGT CCACGCTCTC TCCAAACCCA AGGGCCAGGC AGCATCGCCC
    CAGCGCCACA GCAAATCGCC CGCCACTGAG GCCCACCTGA AGGACCTGTA TGCTAGCGTT
    ATGGGCTATT TTGGAAGAAA AACATCAGCC AATAAAGACG AGCTGACGCC GAAGATGCGG
    CCCATGTCTA ACGATGCTGG CAGCAGCAAC CCCAATGTCA GTGACCTCAT GGACGAGTTC
    ATCCAGGAGA GACTGAGAGC CAGAGGCAAT TCTGGCATGA CGAGGCGCGG CAGTAGCCCA
    GGAAGTCTGG AGATCCCTAA AGATCTGCCA GAGCTGCTGA ACCGTCAGAA CGCCACACGT
    CCCGCAGACG ACCCCGGTGT GCCCTCTGAG TGGACCTCGC CTGCCAGTGC CAGTGGAAGT
    GACCTCATAA GCTCTGACAG CCAATCGGAT TCCTTTAACG CTTTTCAATA TGACAGCAAA
    TTCGAAAATT TCAGCTTCCC CCCTGAGACG TGTGCTTTGG CGTCTGCAGA TCAGGATAGT
    TTGAGTGGGA CAGGACAGAC AGCGGAGGAA CAGGAGCTTT CCAGTCTCAC AACACTTCAC
    ATCGACTCTG AGACCAGCAG CCTTAGTCAG CACGGCCTGT CTGCTGATAC TGTCACTATT
    ACTGGCTCTG AGAGCGCATC TCCAATGCAC TCTCTAGGAG GATCTCGATC GCAGACGCCA
    TCTCCTGCAA CCCTGACCGC TGAACACGTC CAGCATAAAG AGCTGCAGCT GGATGAGAAG
    ATGCACCATT CTGTTCTGCA GACCCCTGAT GACCTTGAAA CCAGCGAGTT TCCCACTGAG
    GACTGTAGTG TAATGGCTGG TGGCACTCTG ACTGGCTGGC ATGCTGATGT CGCAACAGTA
    ATGTGGAGGA GAATGCTTGG GATTCTGGGA GATGTCAACT ATATAAAGGA CCCTGAGATC
    CATGCGCAGG TTTTTGACTA CCTCTGCGAA CTCTGGCAGA ACCTGGCCAA GATCAGAGAC
    AATCTCGGGA TCTCCCTTGA CAACCAGTCG TCTCCGCCAC CACCAGATCT GATTCCACCT
    CTGCGGATTC TGACTCCATG GCTTTTCAAA GCGACTATGT TGGGCGAACG CTACAAGCAG
    GGAAAACTGC ACGCCTACAA GCTCATCTGT AAGATCATGA AGAGAAGACA GGACGTTTCC
    CCAAACTCTG ACTTCCTCAC GCACTTCTAC AACATCATGC ACTGCGGCCT GCTCCACCAA
    GACCAGGATA TTGTGAACAC CATCATTAAG CACTGCTCTC CACGCTTCTT CTCTATTGGA
    CTTCCAGGAG CCACAATGCT CATCCTGGAC TTCATCGTGG CAGCTAGTCG TGTCACTGCC
    TGCTCGTCCC TCAATGCTCC ACGGGTGGAG GCTCAGATCT TGTTGGGTTC CCTGGTTTGC
    CTCCCAAACT TGTACAATGA ACTTCCTGCA CTGCATCCTA CAACAGCCGA CATCATCATG
    ACCAAATTCA CTGATGTCAA GGAGCATATA ATCAAGCACA TCCTGACCTC TGCCAGAGAT
    GAGCCTTCTG CTCCAGCCAG GTGTGTGGCG CTCTGTAGTC TGGGTATTTG GCTGTGTGAA
    GAGCTGGTTC ATGGCACTCA GCATCCGCAG ATCAAAGAAG CGCTTAACGT CATCTGTGTC
    ACTCTCAAGT TCTCCAATAA AAACGTCGCC CTGGTGGCTT CAGACATTTT GCACCTGCTG
    ATAAACTACG TGGATGAACT GGAGAAATTT CCACCCAACA CTCCAAAGAA AATAGTGGAG
    GTTCTTATTG CAACCATCAC TTACCTTCTT CCAGCCACAG AGTCCTCTCC TCATGAGCTG
    GACAAGAGGC TGGTTGTTTC TCTACTGCTG TGCTTGCTGG ACTGGGTGAT GGCTCTACCT
    CCGAAAACAC TCCTCCAGCC GGTCCAAGGA GCCTCGGATA AGGAGAAAAC TGACAAGTCT
    GTCCTCAGCT GCATCTATAA AGTCCTCCAT GGTTGTGTCT ATGGGGCTCA GAGCTTCAGT
    AACCCCAGTT ACTTCCCCAT TCACCTGTCA GACTTAAGCA GCCCAGACTA CGACCCCTTC
    TTACTACTAG AAAACCTTAA GGAGCCGGAG CCACTCCATT CACCTGACTC TGAACGCTCC
    TCCAAACTGC AACCAGTAAC TGAAGTGAGG AGCCGCATTC AGCAAGGCCT TGTTTCAGTT
    GCGGCACGCA CTGTCATATC TCACCTAGTC AACCATCTGG GTCACTATCC CATGAGTGGT
    GGTCCGGCCA CTCTGACGAG CCAGGTGTGC GAGAACCAGG ACAACCCATA CAGTGAGAGC
    GCAGACCTGA CCCCTGAACT CTTTGACGCC CCCAATTTGC AGTTCTTCGT GCTTAATGGC
    ACCACCCTGC TGTCCTACTT ACAAATTCGG GCTGAAGATG GCCTGCCCGG TGGTGGGATG
    TCAGCTGGCC TCACCACCAC CAATGCTTGT GTTCGGGTGA TTGTGAGGGA CATTTCTGGT
    AAACACTCCT GGGACTCAGC TGTTCTGTAT GGTCCACCTC ATTGCATCAC TCCTAGTCAA
    ACTTTGCACC TTTACACGTC CTCTAATCCG AGAGGGGAAC ATAATAGAGA TGAGGACAGT
    TTAGAGAGGG GCAGCCAAGA AGAACCAGAG GATGGAGCTT TTCAGGAAAG CGAGGAGGAC
    TTGGATGAGC ATGAGGAGGC GGAGGTGAAA GAAGACGAGG TGGAGGAGGA AGAACTTGAG
    GAAGAGATTG AGGAGGAAGA GGTTGCACTG GAACTTATGG CCCCGCAGGC CAAACGGCGG
    TGCAGAGAGG TCATCCCGAA CTGGGACTCC CTGCAGGATG GGGAAGACTC CCTAGATGAG
    ATGCTGCAAT ATCTGGGATA CTCCAGCCCA GAGTGTCTGC AACGTACTGG TGGACCTCTC
    AACATACCTG CCCCACCTCC AAACTGTGTG TCCGAGAAAC AGGAGAATGA TGTCATCAAT
    GCCATCCTGA AGCAGTGTGC CGAGGAGCAG GACTTCACAA TTCACCGTGG TAATGAGCTG
    AACATGAGGG CCGTCGTTCA GCAGGAGCCA AGCCCACAGA GACCACAGTC TGCCTTCTAC
    TACTGCAAAC TGCTCCTCAA CATACTAGGC ATGAACTCTT GGGAGAAGAG GAACAGTTTC
    CACCTGCTGA AAAAGAATGA AAAACTACTG AGAGAGCTGA AGAATCTGGA CTCCAGGCAG
    TGTCGTGAAA CACACAAAAT CGCGGTGTTT TACGTGGCAG AGGGACAAGA AGACAAGCAC
    TCCATTCTGT CCAACACAGC CGGGAGTCAG GCGTATGAAG ACTTTGTATC AGGCCTGGGC
    TGGGAGGTGA ACCTGACGAG TCACTGTGGC TTTATGGGTG GCCTGCAGCG GAACAAGAGC
    ACGGGTTTTA CCATGCCTTA CTTTGCCACC TCTACAGTGG AAGTGATGTT CCACGTCTCC
    ACACGCATGC CTCCAGACTC AGACGACTCG CTCACTAAAA AGTTGAGACA CCTGGGTAAT
    GATGAAGTGC ACATCGTGTG GTCTGAGCAC TCGCGGGACT ATCGGAGAGG CGTCATTCCT
    ACTGAGTTTG GAGACGTGCT CATCGTCATC TACCCTATGA AGAACCACAT GTACAGCATC
    CAGATCATCA AGAAACCTGA GGTGCCGTTC TTTGGTCCCT TGTTTGACGG AGCCATAGTG
    GATGGGACTA TATTGCCCAC AGTGGTGCGA GCGACAGCCA TCAACGCCAG CAGAGCTCTG
    AAATCCCTCA TCCCTCTATA CCAAAACTTC TATGAAGAGA GGGCCAGATA TCTGGAGACG
    ATAGTCCAAC ATCACTTGGA GCCCACGACA TTTGAGGACT ACGCCGCTCG TGTCTTTTGT
    CCTGCGCCTT TCCACTATCT TCCGTCTGAA GCAGAGAGAA ATGCTGTGAT TTTTCTGCCC
    CTCCCTAAAC CTCCACTAGA AAACATTGGG CTCACCAAGG TAAAGGGCAA AGGCTTGAAA
    CGGAAGGTCT TTCACGCTAG CTGTGAATGG CTCATGCCTC GAGAGTCAGC TGGCAGAAAG
    TCCAGCAATG CAGGTCGACG GGAGCGACTT AGCCTCGCCA ATGTCACCTC GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149648
    Clone ID Related Accession (Same CDS sequence) XM_021467310.1
    Accession Version XM_021467310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGCCAAA
    AGTCTGGAGT GGAAGTGCAA GGAGAACCAC GAGAAGGGCT TCGCATTTCT GTTCGCCAAC
    TTTAAAAAGT ATTACCTGCC CCATATCTTC CCCAACTTCT CCAAGGAGAC CAGTTTGTAC
    AACCCTATTT TGGAGGTTCC ACCCATGAGA CCAAAGCCTT ACTACATGGT TGTAAAAAGA
    GACCCAGAGA CCAATGAAGC ACTGTACTGC ACTAAAGAGA ACTTCCTCAA TGCCAGAGTC
    ATATTCATCC GCTGGCTGGT CTCCTTCTGG CTGGAGCCGC GGTCGAACAC AGGAACACAG
    ATCCCCGGCA TGGAAGGAGA AAATGTGCCA AAGAATATCC AGAGAGCGGC AGCAGGCCTA
    GCAGCTCGAG GAGAAGATGG CGGCCCACGA CAGGACAGTC TGGATGGTGG TGGTATTGGC
    GAACCAGAGC AGTCGCACTC CAACACCAGC ACCCTGACGG AGAGAGAGCC CAGCTCCTCC
    AGCCTCTGCA GCATGGATGA AGAGCACCTC ACCGACATCG AGGTGGTGCG CCGGGTCCTG
    TGCTCCTCCA GAACCAACGT CAACTTCATC ACAGAGATAT TTCGCCAGGC GTTCCTGCTG
    CCGATGTGTG AGGCTGCAGC CATGCGTAAG GTGGTGCGAG TGTATCAAGA GTGGATTTCT
    CAGCAGGATA AGCCTGTGTT TATGAGACAA CCAGAGGAAG AGCGATTCAC AGGCCAGCTG
    GACTCAACTC ACGAGCAGAG AGCTGATAAA GACGAGGAGG AGGATGTTGG GCTCACGGTG
    TCAGTTCGAA ACCCTAATTG GAGTAGGAGT AAAAGCTCTG ATTCTGAGTA CAATGTTCAC
    GCTGGAGTCC AGGCTACACT ACAGGTTTTT ATCACCAATT CCTCTAATGT CTTCCTTCTG
    GAGGCTGCCA ATGAGCTCAA ATCACTACTG GAAGAGCATG TGGACATGTG CAAGCGCGTG
    CTGAACATTT ACCGCAGTCT GGTCATGCAT GAAACCATGA ACCAGAAGAC CTGGGAGCAG
    ATTTTGCTAG TGTTGCTGCG AGTGACTGAG TGTGTGATGA AGAGGCCTCC CTCCATTATG
    CCTCATGGAA AGAAGAGTAG CACTTTATCA GGCCGTTTGG CTGGAGCCAT TTTTCAGACT
    CTGATTGTGG CCTGGATCAA GGGGAATCTG AACGTGTACA TCTCTCGAGA GCTGTGGGAT
    GACCTGCTGT CTGTCCTGTC CTCTCTCACC TGCTGGGAAG AGCTGGTCAC CGAGTGGTCC
    CTCACAATGG AGACACTGAC CAAGGTGCTG GCACGAAACC TTTACAGCCT GGACCTGAAC
    GAGCTGCCCT TGGACAAACT TAGTGAGCAG AAGCAGAAGA AGCACAAAGG CAAAGGTGGT
    ATCCATGAGG GCCAGAAAAT CACTGTGGAT CGGTCGTTCT CTAAAGGCTG GAGTCGAGAT
    CCTCCCGGTC AGGCGGCGGC CATGAGACAG CGCAGCGCCA CCACCGCTGG ATCACCAGGC
    ATCGAGAAGG CCAGAAGCAT CGTCCGACAG AAGACAGTCG CTCTTCGTAG TTGTTCTACG
    GGCGACAGTC TGCTGTCCTC AGCCTTTATC CGCAGTGCTA AAAGCGCACC TGTCCTGATC
    CACCCCGTAC ACCCTCTCCT GCCTGACTCT GTCCTCACTC CTCTAGCTGA CGAGCTGTCA
    GACCTGGAGG AGCCTCCTAT GATCCAGCGT GGGCCACGGA ACCGCCACTG CTCTCAGAGC
    GAGGAGACGC CGACGTCTGA AGTTTTTTCG GCCTCCGGTG AACTGGAGCA GCCTCCGCTT
    CCCCGCAGTA GCAGTGCCTC CGACATCATG GAGCCCTTCA TCGCAGAACG GGCCAAAGGT
    GCACTTCCTG TCTCTGACAG TCCCTCTCAC TCCCCCCACT TTACAGCCAC CACCACCACC
    ATTGAGTTCC CCCACAGTTC CCCAAACCCA CAGCAGCTCA CTGACCCACA GCAGCTCCCC
    GACAGCCAGG CGTCCCTGGA GGTGGCCGAA AGTATCTATG ACCATCTCTG CCAAATGCCA
    GGCCAGCAGC CCCCCTTTAT CCCCGATTGG TCCTGTCCAG CCGCGGCCTC GGCCAATCAA
    CCCGAGGGCG AAGCGGAGGA GGACGTCTTC AGTGCTTTTC GGGAGTTTTG TCACAGGGAG
    AATGTGAAAA TGGAAGCAGG TGCAGATTTA AAGCAGGTGC AGTTTGAGGG CGATTGGGCT
    CTAGAATGGG ATAGAGAGAT GGAGCAGAGC GAGAAGAGTT TGTATGAGTG CTTAGAGGAG
    TGTGATTGGG ATTTGAGTGT TAGTCCGCAG CCAGCAGAGG ACGGTAAAGG AGCACAGCTT
    CAGCGGCAGG ACGCCACCGA CAGGGCTAGC GAGAGTGAGG TTAGTCCACA GGCTTCGCAT
    GGACCCATGT TGTCAAATAT TGATCACAAG AAACGCCACA GCAGTGCGGT CCATGTCAGT
    TTCCGGCCCT CCACAGAGTC TGTGCTGTTC CACAACCCTT TGGACCCCAA GGAGGCACAC
    TGGAAAACCC GGCTTCGCCG TCTCGGGCAC TTCAGCAGCC ACTCGGTTGG GGCGAAAGGT
    GTAGGGGAGG GGGTCACCTG TTGTGAAGTG GTGGAGCGGG AAAGAGGCTC CATCAGCGGG
    GCTAGACGTG GACGACTTGG TCGTCCGACT CTTCGGCCTC GAGGGTCCCG ATCTAGATCT
    CAGGAGTCAG GGTGCAGCCC CTCACGACAG CAGCAGGTGG CTCTGTTGGG TGGGGTGTAT
    AAGTCTATGG TCCACGCTCT CTCCAAACCC AAGGGCCAGG CAGCATCGCC CCAGCGCCAC
    AGCAAATCGC CCGCCACTGA GGCCCACCTG AAGGACCTGT ATGCTAGCGT TATGGGCTAT
    TTTGGAAGAA AAACATCAGC CAATAAAGAC GAGCTGACGC CGAAGATGCG GCCCATGTCT
    AACGATGCTG GCAGCAGCAA CCCCAATGTC AGTGACCTCA TGGACGAGTT CATCCAGGAG
    AGACTGAGAG CCAGAGGCAA TTCTGGCATG ACGAGGCGCG GCAGTAGCCC AGGAAGTCTG
    GAGATCCCTA AAGATCTGCC AGAGCTGCTG AACCGTCAGA ACGCCACACG TCCCGCAGAC
    GACCCCGGTG TGCCCTCTGA GTGGACCTCG CCTGCCAGTG CCAGTGGAAG TGACCTCATA
    AGCTCTGACA GCCAATCGGA TTCCTTTAAC GCTTTTCAAT ATGACAGCAA ATTCGAAAAT
    TTCAGCTTCC CCCCTGAGAC GTGTGCTTTG GCGTCTGCAG ATCAGGATAG TTTGAGTGGG
    ACAGGACAGA CAGCGGAGGA ACAGGAGCTT TCCAGTCTCA CAACACTTCA CATCGACTCT
    GAGACCAGCA GCCTTAGTCA GCACGGCCTG TCTGCTGATA CTGTCACTAT TACTGGCTCT
    GAGAGCGCAT CTCCAATGCA CTCTCTAGGA GGATCTCGAT CGCAGACGCC ATCTCCTGCA
    ACCCTGACCG CTGAACACGT CCAGCATAAA GAGCTGCAGC TGGATGAGAA GATGCACCAT
    TCTGTTCTGC AGACCCCTGA TGACCTTGAA ACCAGCGAGT TTCCCACTGA GGACTGTAGT
    GTAATGGCTG GTGGCACTCT GACTGGCTGG CATGCTGATG TCGCAACAGT AATGTGGAGG
    AGAATGCTTG GGATTCTGGG AGATGTCAAC TATATAAAGG ACCCTGAGAT CCATGCGCAG
    GTTTTTGACT ACCTCTGCGA ACTCTGGCAG AACCTGGCCA AGATCAGAGA CAATCTCGGG
    ATCTCCCTTG ACAACCAGTC GTCTCCGCCA CCACCAGATC TGATTCCACC TCTGCGGATT
    CTGACTCCAT GGCTTTTCAA AGCGACTATG TTGGGCGAAC GCTACAAGCA GGGAAAACTG
    CACGCCTACA AGCTCATCTG TAAGATCATG AAGAGAAGAC AGGACGTTTC CCCAAACTCT
    GACTTCCTCA CGCACTTCTA CAACATCATG CACTGCGGCC TGCTCCACCA AGACCAGGAT
    ATTGTGAACA CCATCATTAA GCACTGCTCT CCACGCTTCT TCTCTATTGG ACTTCCAGGA
    GCCACAATGC TCATCCTGGA CTTCATCGTG GCAGCTAGTC GTGTCACTGC CTGCTCGTCC
    CTCAATGCTC CACGGGTGGA GGCTCAGATC TTGTTGGGTT CCCTGGTTTG CCTCCCAAAC
    TTGTACAATG AACTTCCTGC ACTGCATCCT ACAACAGCCG ACATCATCAT GACCAAATTC
    ACTGATGTCA AGGAGCATAT AATCAAGCAC ATCCTGACCT CTGCCAGAGA TGAGCCTTCT
    GCTCCAGCCA GGTGTGTGGC GCTCTGTAGT CTGGGTATTT GGCTGTGTGA AGAGCTGGTT
    CATGGCACTC AGCATCCGCA GATCAAAGAA GCGCTTAACG TCATCTGTGT CACTCTCAAG
    TTCTCCAATA AAAACGTCGC CCTGGTGGCT TCAGACATTT TGCACCTGCT GATAAACTAC
    GTGGATGAAC TGGAGAAATT TCCACCCAAC ACTCCAAAGA AAATAGTGGA GGTTCTTATT
    GCAACCATCA CTTACCTTCT TCCAGCCACA GAGTCCTCTC CTCATGAGCT GGACAAGAGG
    CTGGTTGTTT CTCTACTGCT GTGCTTGCTG GACTGGGTGA TGGCTCTACC TCCGAAAACA
    CTCCTCCAGC CGGTCCAAGG AGCCTCGGAT AAGGAGAAAA CTGACAAGTC TGTCCTCAGC
    TGCATCTATA AAGTCCTCCA TGGTTGTGTC TATGGGGCTC AGAGCTTCAG TAACCCCAGT
    TACTTCCCCA TTCACCTGTC AGACTTAAGC AGCCCAGACT ACGACCCCTT CTTACTACTA
    GAAAACCTTA AGGAGCCGGA GCCACTCCAT TCACCTGACT CTGAACGCTC CTCCAAACTG
    CAACCAGTAA CTGAAGTGAG GAGCCGCATT CAGCAAGGCC TTGTTTCAGT TGCGGCACGC
    ACTGTCATAT CTCACCTAGT CAACCATCTG GGTCACTATC CCATGAGTGG TGGTCCGGCC
    ACTCTGACGA GCCAGGTGTG CGAGAACCAG GACAACCCAT ACAGTGAGAG CGCAGACCTG
    ACCCCTGAAC TCTTTGACGC CCCCAATTTG CAGTTCTTCG TGCTTAATGG CACCACCCTG
    CTGTCCTACT TACAAATTCG GGCTGAAGAT GGCCTGCCCG GTGGTGGGAT GTCAGCTGGC
    CTCACCACCA CCAATGCTTG TGTTCGGGTG ATTGTGAGGG ACATTTCTGG TAAACACTCC
    TGGGACTCAG CTGTTCTGTA TGGTCCACCT CATTGCATCA CTCCTAGTCA AACTTTGCAC
    CTTTACACGT CCTCTAATCC GAGAGGGGAA CATAATAGAG ATGAGGACAG TTTAGAGAGG
    GGCAGCCAAG AAGAACCAGA GGATGGAGCT TTTCAGGAAA GCGAGGAGGA CTTGGATGAG
    CATGAGGAGG CGGAGGTGAA AGAAGACGAG GTGGAGGAGG AAGAACTTGA GGAAGAGATT
    GAGGAGGAAG AGGTTGCACT GGAACTTATG GCCCCGCAGG CCAAACGGCG GTGCAGAGAG
    GTCATCCCGA ACTGGGACTC CCTGCAGGAT GGGGAAGACT CCCTAGATGA GATGCTGCAA
    TATCTGGGAT ACTCCAGCCC AGAGTGTCTG CAACGTACTG GTGGACCTCT CAACATACCT
    GCCCCACCTC CAAACTGTGT GTCCGAGAAA CAGGAGAATG ATGTCATCAA TGCCATCCTG
    AAGCAGTGTG CCGAGGAGCA GGACTTCACA ATTCACCGTG GTAATGAGCT GAACATGAGG
    GCCGTCGTTC AGCAGGAGCC AAGCCCACAG AGACCACAGT CTGCCTTCTA CTACTGCAAA
    CTGCTCCTCA ACATACTAGG CATGAACTCT TGGGAGAAGA GGAACAGTTT CCACCTGCTG
    AAAAAGAATG AAAAACTACT GAGAGAGCTG AAGAATCTGG ACTCCAGGCA GTGTCGTGAA
    ACACACAAAA TCGCGGTGTT TTACGTGGCA GAGGGACAAG AAGACAAGCA CTCCATTCTG
    TCCAACACAG CCGGGAGTCA GGCGTATGAA GACTTTGTAT CAGGCCTGGG CTGGGAGGTG
    AACCTGACGA GTCACTGTGG CTTTATGGGT GGCCTGCAGC GGAACAAGAG CACGGGTTTT
    ACCATGCCTT ACTTTGCCAC CTCTACAGTG GAAGTGATGT TCCACGTCTC CACACGCATG
    CCTCCAGACT CAGACGACTC GCTCACTAAA AAGTTGAGAC ACCTGGGTAA TGATGAAGTG
    CACATCGTGT GGTCTGAGCA CTCGCGGGAC TATCGGAGAG GCGTCATTCC TACTGAGTTT
    GGAGACGTGC TCATCGTCAT CTACCCTATG AAGAACCACA TGTACAGCAT CCAGATCATC
    AAGAAACCTG AGGTGCCGTT CTTTGGTCCC TTGTTTGACG GAGCCATAGT GGATGGGACT
    ATATTGCCCA CAGTGGTGCG AGCGACAGCC ATCAACGCCA GCAGAGCTCT GAAATCCCTC
    ATCCCTCTAT ACCAAAACTT CTATGAAGAG AGGGCCAGAT ATCTGGAGAC GATAGTCCAA
    CATCACTTGG AGCCCACGAC ATTTGAGGAC TACGCCGCTC GTGTCTTTTG TCCTGCGCCT
    TTCCACTATC TTCCGTCTGA AGCAGAGAGA AATGCTGTGA TTTTTCTGCC CCTCCCTAAA
    CCTCCACTAG AAAACATTGG GCTCACCAAG GTAAAGGGCA AAGGCTTGAA ACGGAAGGTC
    TTTCACGCTA GCTGTGAATG GCTCATGCCT CGAGAGTCAG CTGGCAGAAA GTCCAGCAAT
    GCAGGTCGAC GGGAGCGACT TAGCCTCGCC AATGTCACCT CGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322985.1
    CDS657..8204
    Translation

    Target ORF information:

    RefSeq Version XM_021467310.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGCCAAA
    AGTCTGGAGT GGAAGTGCAA GGAGAACCAC GAGAAGGGCT TCGCATTTCT GTTCGCCAAC
    TTTAAAAAGT ATTACCTGCC CCATATCTTC CCCAACTTCT CCAAGGAGAC CAGTTTGTAC
    AACCCTATTT TGGAGGTTCC ACCCATGAGA CCAAAGCCTT ACTACATGGT TGTAAAAAGA
    GACCCAGAGA CCAATGAAGC ACTGTACTGC ACTAAAGAGA ACTTCCTCAA TGCCAGAGTC
    ATATTCATCC GCTGGCTGGT CTCCTTCTGG CTGGAGCCGC GGTCGAACAC AGGAACACAG
    ATCCCCGGCA TGGAAGGAGA AAATGTGCCA AAGAATATCC AGAGAGCGGC AGCAGGCCTA
    GCAGCTCGAG GAGAAGATGG CGGCCCACGA CAGGACAGTC TGGATGGTGG TGGTATTGGC
    GAACCAGAGC AGTCGCACTC CAACACCAGC ACCCTGACGG AGAGAGAGCC CAGCTCCTCC
    AGCCTCTGCA GCATGGATGA AGAGCACCTC ACCGACATCG AGGTGGTGCG CCGGGTCCTG
    TGCTCCTCCA GAACCAACGT CAACTTCATC ACAGAGATAT TTCGCCAGGC GTTCCTGCTG
    CCGATGTGTG AGGCTGCAGC CATGCGTAAG GTGGTGCGAG TGTATCAAGA GTGGATTTCT
    CAGCAGGATA AGCCTGTGTT TATGAGACAA CCAGAGGAAG AGCGATTCAC AGGCCAGCTG
    GACTCAACTC ACGAGCAGAG AGCTGATAAA GACGAGGAGG AGGATGTTGG GCTCACGGTG
    TCAGTTCGAA ACCCTAATTG GAGTAGGAGT AAAAGCTCTG ATTCTGAGTA CAATGTTCAC
    GCTGGAGTCC AGGCTACACT ACAGGTTTTT ATCACCAATT CCTCTAATGT CTTCCTTCTG
    GAGGCTGCCA ATGAGCTCAA ATCACTACTG GAAGAGCATG TGGACATGTG CAAGCGCGTG
    CTGAACATTT ACCGCAGTCT GGTCATGCAT GAAACCATGA ACCAGAAGAC CTGGGAGCAG
    ATTTTGCTAG TGTTGCTGCG AGTGACTGAG TGTGTGATGA AGAGGCCTCC CTCCATTATG
    CCTCATGGAA AGAAGAGTAG CACTTTATCA GGCCGTTTGG CTGGAGCCAT TTTTCAGACT
    CTGATTGTGG CCTGGATCAA GGGGAATCTG AACGTGTACA TCTCTCGAGA GCTGTGGGAT
    GACCTGCTGT CTGTCCTGTC CTCTCTCACC TGCTGGGAAG AGCTGGTCAC CGAGTGGTCC
    CTCACAATGG AGACACTGAC CAAGGTGCTG GCACGAAACC TTTACAGCCT GGACCTGAAC
    GAGCTGCCCT TGGACAAACT TAGTGAGCAG AAGCAGAAGA AGCACAAAGG CAAAGGTGGT
    ATCCATGAGG GCCAGAAAAT CACTGTGGAT CGGTCGTTCT CTAAAGGCTG GAGTCGAGAT
    CCTCCCGGTC AGGCGGCGGC CATGAGACAG CGCAGCGCCA CCACCGCTGG ATCACCAGGC
    ATCGAGAAGG CCAGAAGCAT CGTCCGACAG AAGACAGTCG CTCTTCGTAG TTGTTCTACG
    GGCGACAGTC TGCTGTCCTC AGCCTTTATC CGCAGTGCTA AAAGCGCACC TGTCCTGATC
    CACCCCGTAC ACCCTCTCCT GCCTGACTCT GTCCTCACTC CTCTAGCTGA CGAGCTGTCA
    GACCTGGAGG AGCCTCCTAT GATCCAGCGT GGGCCACGGA ACCGCCACTG CTCTCAGAGC
    GAGGAGACGC CGACGTCTGA AGTTTTTTCG GCCTCCGGTG AACTGGAGCA GCCTCCGCTT
    CCCCGCAGTA GCAGTGCCTC CGACATCATG GAGCCCTTCA TCGCAGAACG GGCCAAAGGT
    GCACTTCCTG TCTCTGACAG TCCCTCTCAC TCCCCCCACT TTACAGCCAC CACCACCACC
    ATTGAGTTCC CCCACAGTTC CCCAAACCCA CAGCAGCTCA CTGACCCACA GCAGCTCCCC
    GACAGCCAGG CGTCCCTGGA GGTGGCCGAA AGTATCTATG ACCATCTCTG CCAAATGCCA
    GGCCAGCAGC CCCCCTTTAT CCCCGATTGG TCCTGTCCAG CCGCGGCCTC GGCCAATCAA
    CCCGAGGGCG AAGCGGAGGA GGACGTCTTC AGTGCTTTTC GGGAGTTTTG TCACAGGGAG
    AATGTGAAAA TGGAAGCAGG TGCAGATTTA AAGCAGGTGC AGTTTGAGGG CGATTGGGCT
    CTAGAATGGG ATAGAGAGAT GGAGCAGAGC GAGAAGAGTT TGTATGAGTG CTTAGAGGAG
    TGTGATTGGG ATTTGAGTGT TAGTCCGCAG CCAGCAGAGG ACGGTAAAGG AGCACAGCTT
    CAGCGGCAGG ACGCCACCGA CAGGGCTAGC GAGAGTGAGG TTAGTCCACA GGCTTCGCAT
    GGACCCATGT TGTCAAATAT TGATCACAAG AAACGCCACA GCAGTGCGGT CCATGTCAGT
    TTCCGGCCCT CCACAGAGTC TGTGCTGTTC CACAACCCTT TGGACCCCAA GGAGGCACAC
    TGGAAAACCC GGCTTCGCCG TCTCGGGCAC TTCAGCAGCC ACTCGGTTGG GGCGAAAGGT
    GTAGGGGAGG GGGTCACCTG TTGTGAAGTG GTGGAGCGGG AAAGAGGCTC CATCAGCGGG
    GCTAGACGTG GACGACTTGG TCGTCCGACT CTTCGGCCTC GAGGGTCCCG ATCTAGATCT
    CAGGAGTCAG GGTGCAGCCC CTCACGACAG CAGCAGGTGG CTCTGTTGGG TGGGGTGTAT
    AAGTCTATGG TCCACGCTCT CTCCAAACCC AAGGGCCAGG CAGCATCGCC CCAGCGCCAC
    AGCAAATCGC CCGCCACTGA GGCCCACCTG AAGGACCTGT ATGCTAGCGT TATGGGCTAT
    TTTGGAAGAA AAACATCAGC CAATAAAGAC GAGCTGACGC CGAAGATGCG GCCCATGTCT
    AACGATGCTG GCAGCAGCAA CCCCAATGTC AGTGACCTCA TGGACGAGTT CATCCAGGAG
    AGACTGAGAG CCAGAGGCAA TTCTGGCATG ACGAGGCGCG GCAGTAGCCC AGGAAGTCTG
    GAGATCCCTA AAGATCTGCC AGAGCTGCTG AACCGTCAGA ACGCCACACG TCCCGCAGAC
    GACCCCGGTG TGCCCTCTGA GTGGACCTCG CCTGCCAGTG CCAGTGGAAG TGACCTCATA
    AGCTCTGACA GCCAATCGGA TTCCTTTAAC GCTTTTCAAT ATGACAGCAA ATTCGAAAAT
    TTCAGCTTCC CCCCTGAGAC GTGTGCTTTG GCGTCTGCAG ATCAGGATAG TTTGAGTGGG
    ACAGGACAGA CAGCGGAGGA ACAGGAGCTT TCCAGTCTCA CAACACTTCA CATCGACTCT
    GAGACCAGCA GCCTTAGTCA GCACGGCCTG TCTGCTGATA CTGTCACTAT TACTGGCTCT
    GAGAGCGCAT CTCCAATGCA CTCTCTAGGA GGATCTCGAT CGCAGACGCC ATCTCCTGCA
    ACCCTGACCG CTGAACACGT CCAGCATAAA GAGCTGCAGC TGGATGAGAA GATGCACCAT
    TCTGTTCTGC AGACCCCTGA TGACCTTGAA ACCAGCGAGT TTCCCACTGA GGACTGTAGT
    GTAATGGCTG GTGGCACTCT GACTGGCTGG CATGCTGATG TCGCAACAGT AATGTGGAGG
    AGAATGCTTG GGATTCTGGG AGATGTCAAC TATATAAAGG ACCCTGAGAT CCATGCGCAG
    GTTTTTGACT ACCTCTGCGA ACTCTGGCAG AACCTGGCCA AGATCAGAGA CAATCTCGGG
    ATCTCCCTTG ACAACCAGTC GTCTCCGCCA CCACCAGATC TGATTCCACC TCTGCGGATT
    CTGACTCCAT GGCTTTTCAA AGCGACTATG TTGGGCGAAC GCTACAAGCA GGGAAAACTG
    CACGCCTACA AGCTCATCTG TAAGATCATG AAGAGAAGAC AGGACGTTTC CCCAAACTCT
    GACTTCCTCA CGCACTTCTA CAACATCATG CACTGCGGCC TGCTCCACCA AGACCAGGAT
    ATTGTGAACA CCATCATTAA GCACTGCTCT CCACGCTTCT TCTCTATTGG ACTTCCAGGA
    GCCACAATGC TCATCCTGGA CTTCATCGTG GCAGCTAGTC GTGTCACTGC CTGCTCGTCC
    CTCAATGCTC CACGGGTGGA GGCTCAGATC TTGTTGGGTT CCCTGGTTTG CCTCCCAAAC
    TTGTACAATG AACTTCCTGC ACTGCATCCT ACAACAGCCG ACATCATCAT GACCAAATTC
    ACTGATGTCA AGGAGCATAT AATCAAGCAC ATCCTGACCT CTGCCAGAGA TGAGCCTTCT
    GCTCCAGCCA GGTGTGTGGC GCTCTGTAGT CTGGGTATTT GGCTGTGTGA AGAGCTGGTT
    CATGGCACTC AGCATCCGCA GATCAAAGAA GCGCTTAACG TCATCTGTGT CACTCTCAAG
    TTCTCCAATA AAAACGTCGC CCTGGTGGCT TCAGACATTT TGCACCTGCT GATAAACTAC
    GTGGATGAAC TGGAGAAATT TCCACCCAAC ACTCCAAAGA AAATAGTGGA GGTTCTTATT
    GCAACCATCA CTTACCTTCT TCCAGCCACA GAGTCCTCTC CTCATGAGCT GGACAAGAGG
    CTGGTTGTTT CTCTACTGCT GTGCTTGCTG GACTGGGTGA TGGCTCTACC TCCGAAAACA
    CTCCTCCAGC CGGTCCAAGG AGCCTCGGAT AAGGAGAAAA CTGACAAGTC TGTCCTCAGC
    TGCATCTATA AAGTCCTCCA TGGTTGTGTC TATGGGGCTC AGAGCTTCAG TAACCCCAGT
    TACTTCCCCA TTCACCTGTC AGACTTAAGC AGCCCAGACT ACGACCCCTT CTTACTACTA
    GAAAACCTTA AGGAGCCGGA GCCACTCCAT TCACCTGACT CTGAACGCTC CTCCAAACTG
    CAACCAGTAA CTGAAGTGAG GAGCCGCATT CAGCAAGGCC TTGTTTCAGT TGCGGCACGC
    ACTGTCATAT CTCACCTAGT CAACCATCTG GGTCACTATC CCATGAGTGG TGGTCCGGCC
    ACTCTGACGA GCCAGGTGTG CGAGAACCAG GACAACCCAT ACAGTGAGAG CGCAGACCTG
    ACCCCTGAAC TCTTTGACGC CCCCAATTTG CAGTTCTTCG TGCTTAATGG CACCACCCTG
    CTGTCCTACT TACAAATTCG GGCTGAAGAT GGCCTGCCCG GTGGTGGGAT GTCAGCTGGC
    CTCACCACCA CCAATGCTTG TGTTCGGGTG ATTGTGAGGG ACATTTCTGG TAAACACTCC
    TGGGACTCAG CTGTTCTGTA TGGTCCACCT CATTGCATCA CTCCTAGTCA AACTTTGCAC
    CTTTACACGT CCTCTAATCC GAGAGGGGAA CATAATAGAG ATGAGGACAG TTTAGAGAGG
    GGCAGCCAAG AAGAACCAGA GGATGGAGCT TTTCAGGAAA GCGAGGAGGA CTTGGATGAG
    CATGAGGAGG CGGAGGTGAA AGAAGACGAG GTGGAGGAGG AAGAACTTGA GGAAGAGATT
    GAGGAGGAAG AGGTTGCACT GGAACTTATG GCCCCGCAGG CCAAACGGCG GTGCAGAGAG
    GTCATCCCGA ACTGGGACTC CCTGCAGGAT GGGGAAGACT CCCTAGATGA GATGCTGCAA
    TATCTGGGAT ACTCCAGCCC AGAGTGTCTG CAACGTACTG GTGGACCTCT CAACATACCT
    GCCCCACCTC CAAACTGTGT GTCCGAGAAA CAGGAGAATG ATGTCATCAA TGCCATCCTG
    AAGCAGTGTG CCGAGGAGCA GGACTTCACA ATTCACCGTG GTAATGAGCT GAACATGAGG
    GCCGTCGTTC AGCAGGAGCC AAGCCCACAG AGACCACAGT CTGCCTTCTA CTACTGCAAA
    CTGCTCCTCA ACATACTAGG CATGAACTCT TGGGAGAAGA GGAACAGTTT CCACCTGCTG
    AAAAAGAATG AAAAACTACT GAGAGAGCTG AAGAATCTGG ACTCCAGGCA GTGTCGTGAA
    ACACACAAAA TCGCGGTGTT TTACGTGGCA GAGGGACAAG AAGACAAGCA CTCCATTCTG
    TCCAACACAG CCGGGAGTCA GGCGTATGAA GACTTTGTAT CAGGCCTGGG CTGGGAGGTG
    AACCTGACGA GTCACTGTGG CTTTATGGGT GGCCTGCAGC GGAACAAGAG CACGGGTTTT
    ACCATGCCTT ACTTTGCCAC CTCTACAGTG GAAGTGATGT TCCACGTCTC CACACGCATG
    CCTCCAGACT CAGACGACTC GCTCACTAAA AAGTTGAGAC ACCTGGGTAA TGATGAAGTG
    CACATCGTGT GGTCTGAGCA CTCGCGGGAC TATCGGAGAG GCGTCATTCC TACTGAGTTT
    GGAGACGTGC TCATCGTCAT CTACCCTATG AAGAACCACA TGTACAGCAT CCAGATCATC
    AAGAAACCTG AGGTGCCGTT CTTTGGTCCC TTGTTTGACG GAGCCATAGT GGATGGGACT
    ATATTGCCCA CAGTGGTGCG AGCGACAGCC ATCAACGCCA GCAGAGCTCT GAAATCCCTC
    ATCCCTCTAT ACCAAAACTT CTATGAAGAG AGGGCCAGAT ATCTGGAGAC GATAGTCCAA
    CATCACTTGG AGCCCACGAC ATTTGAGGAC TACGCCGCTC GTGTCTTTTG TCCTGCGCCT
    TTCCACTATC TTCCGTCTGA AGCAGAGAGA AATGCTGTGA TTTTTCTGCC CCTCCCTAAA
    CCTCCACTAG AAAACATTGG GCTCACCAAG GTAAAGGGCA AAGGCTTGAA ACGGAAGGTC
    TTTCACGCTA GCTGTGAATG GCTCATGCCT CGAGAGTCAG CTGGCAGAAA GTCCAGCAAT
    GCAGGTCGAC GGGAGCGACT TAGCCTCGCC AATGTCACCT CGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149649
    Clone ID Related Accession (Same CDS sequence) XM_021467311.1
    Accession Version XM_021467311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGGTG CACTTCCTGT CTCTGACAGT CCCTCTCACT CCCCCCACTT TACAGCCACC
    ACCACCACCA TTGAGTTCCC CCACAGTTCC CCAAACCCAC AGCAGCTCAC TGACCCACAG
    CAGCTCCCCG ACAGCCAGGC GTCCCTGGAG GTGGCCGAAA GTATCTATGA CCATCTCTGC
    CAAATGCCAG GCCAGCAGCC CCCCTTTATC CCCGATTGGT CCTGTCCAGC CGCGGCCTCG
    GCCAATCAAC CCGAGGGCGA AGCGGAGGAG GACGTCTTCA GTGCTTTTCG GGAGTTTTGT
    CACAGGGAGA ATGTGAAAAT GGAAGCAGGT GCAGATTTAA AGCAGGTGCA GTTTGAGGGC
    GATTGGGCTC TAGAATGGGA TAGAGAGATG GAGCAGAGCG AGAAGAGTTT GTATGAGTGC
    TTAGAGGAGT GTGATTGGGA TTTGAGTGTT AGTCCGCAGC CAGCAGAGGA CGGTAAAGGA
    GCACAGCTTC AGCGGCAGGA CGCCACCGAC AGGGCTAGCG AGAGTGAGGT TAGTCCACAG
    GCTTCGCATG GACCCATGTT GTCAAATATT GATCACAAGA AACGCCACAG CAGTGCGGTC
    CATGTCAGTT TCCGGCCCTC CACAGAGTCT GTGCTGTTCC ACAACCCTTT GGACCCCAAG
    GAGGCACACT GGAAAACCCG GCTTCGCCGT CTCGGGCACT TCAGCAGCCA CTCGGTTGGG
    GCGAAAGGTG TAGGGGAGGG GGTCACCTGT TGTGAAGTGG TGGAGCGGGA AAGAGGCTCC
    ATCAGCGGGG CTAGACGTGG ACGACTTGGT CGTCCGACTC TTCGGCCTCG AGGGTCCCGA
    TCTAGATCTC AGGAGTCAGG GTGCAGCCCC TCACGACAGC AGCAGGTGGC TCTGTTGGGT
    GGGGTGTATA AGTCTATGGT CCACGCTCTC TCCAAACCCA AGGGCCAGGC AGCATCGCCC
    CAGCGCCACA GCAAATCGCC CGCCACTGAG GCCCACCTGA AGGACCTGTA TGCTAGCGTT
    ATGGGCTATT TTGGAAGAAA AACATCAGCC AATAAAGACG AGCTGACGCC GAAGATGCGG
    CCCATGTCTA ACGATGCTGG CAGCAGCAAC CCCAATGTCA GTGACCTCAT GGACGAGTTC
    ATCCAGGAGA GACTGAGAGC CAGAGGCAAT TCTGGCATGA CGAGGCGCGG CAGTAGCCCA
    GGAAGTCTGG AGATCCCTAA AGATCTGCCA GAGCTGCTGA ACCGTCAGAA CGCCACACGT
    CCCGCAGACG ACCCCGGTGT GCCCTCTGAG TGGACCTCGC CTGCCAGTGC CAGTGGAAGT
    GACCTCATAA GCTCTGACAG CCAATCGGAT TCCTTTAACG CTTTTCAATA TGACAGCAAA
    TTCGAAAATT TCAGCTTCCC CCCTGAGACG TGTGCTTTGG CGTCTGCAGA TCAGGATAGT
    TTGAGTGGGA CAGGACAGAC AGCGGAGGAA CAGGAGCTTT CCAGTCTCAC AACACTTCAC
    ATCGACTCTG AGACCAGCAG CCTTAGTCAG CACGGCCTGT CTGCTGATAC TGTCACTATT
    ACTGGCTCTG AGAGCGCATC TCCAATGCAC TCTCTAGGAG GATCTCGATC GCAGACGCCA
    TCTCCTGCAA CCCTGACCGC TGAACACGTC CAGCATAAAG AGCTGCAGCT GGATGAGAAG
    ATGCACCATT CTGTTCTGCA GACCCCTGAT GACCTTGAAA CCAGCGAGTT TCCCACTGAG
    GACTGTAGTG TAATGGCTGG TGGCACTCTG ACTGGCTGGC ATGCTGATGT CGCAACAGTA
    ATGTGGAGGA GAATGCTTGG GATTCTGGGA GATGTCAACT ATATAAAGGA CCCTGAGATC
    CATGCGCAGG TTTTTGACTA CCTCTGCGAA CTCTGGCAGA ACCTGGCCAA GATCAGAGAC
    AATCTCGGGA TCTCCCTTGA CAACCAGTCG TCTCCGCCAC CACCAGATCT GATTCCACCT
    CTGCGGATTC TGACTCCATG GCTTTTCAAA GCGACTATGT TGGGCGAACG CTACAAGCAG
    GGAAAACTGC ACGCCTACAA GCTCATCTGT AAGATCATGA AGAGAAGACA GGACGTTTCC
    CCAAACTCTG ACTTCCTCAC GCACTTCTAC AACATCATGC ACTGCGGCCT GCTCCACCAA
    GACCAGGATA TTGTGAACAC CATCATTAAG CACTGCTCTC CACGCTTCTT CTCTATTGGA
    CTTCCAGGAG CCACAATGCT CATCCTGGAC TTCATCGTGG CAGCTAGTCG TGTCACTGCC
    TGCTCGTCCC TCAATGCTCC ACGGGTGGAG GCTCAGATCT TGTTGGGTTC CCTGGTTTGC
    CTCCCAAACT TGTACAATGA ACTTCCTGCA CTGCATCCTA CAACAGCCGA CATCATCATG
    ACCAAATTCA CTGATGTCAA GGAGCATATA ATCAAGCACA TCCTGACCTC TGCCAGAGAT
    GAGCCTTCTG CTCCAGCCAG GTGTGTGGCG CTCTGTAGTC TGGGTATTTG GCTGTGTGAA
    GAGCTGGTTC ATGGCACTCA GCATCCGCAG ATCAAAGAAG CGCTTAACGT CATCTGTGTC
    ACTCTCAAGT TCTCCAATAA AAACGTCGCC CTGGTGGCTT CAGACATTTT GCACCTGCTG
    ATAAACTACG TGGATGAACT GGAGAAATTT CCACCCAACA CTCCAAAGAA AATAGTGGAG
    GTTCTTATTG CAACCATCAC TTACCTTCTT CCAGCCACAG AGTCCTCTCC TCATGAGCTG
    GACAAGAGGC TGGTTGTTTC TCTACTGCTG TGCTTGCTGG ACTGGGTGAT GGCTCTACCT
    CCGAAAACAC TCCTCCAGCC GGTCCAAGGA GCCTCGGATA AGGAGAAAAC TGACAAGTCT
    GTCCTCAGCT GCATCTATAA AGTCCTCCAT GGTTGTGTCT ATGGGGCTCA GAGCTTCAGT
    AACCCCAGTT ACTTCCCCAT TCACCTGTCA GACTTAAGCA GCCCAGACTA CGACCCCTTC
    TTACTACTAG AAAACCTTAA GGAGCCGGAG CCACTCCATT CACCTGACTC TGAACGCTCC
    TCCAAACTGC AACCAGTAAC TGAAGTGAGG AGCCGCATTC AGCAAGGCCT TGTTTCAGTT
    GCGGCACGCA CTGTCATATC TCACCTAGTC AACCATCTGG GTCACTATCC CATGAGTGGT
    GGTCCGGCCA CTCTGACGAG CCAGGTGTGC GAGAACCAGG ACAACCCATA CAGTGAGAGC
    GCAGACCTGA CCCCTGAACT CTTTGACGCC CCCAATTTGC AGTTCTTCGT GCTTAATGGC
    ACCACCCTGC TGTCCTACTT ACAAATTCGG GCTGAAGATG GCCTGCCCGG TGGTGGGATG
    TCAGCTGGCC TCACCACCAC CAATGCTTGT GTTCGGGTGA TTGTGAGGGA CATTTCTGGT
    AAACACTCCT GGGACTCAGC TGTTCTGTAT GGTCCACCTC ATTGCATCAC TCCTAGTCAA
    ACTTTGCACC TTTACACGTC CTCTAATCCG AGAGGGGAAC ATAATAGAGA TGAGGACAGT
    TTAGAGAGGG GCAGCCAAGA AGAACCAGAG GATGGAGCTT TTCAGGAAAG CGAGGAGGAC
    TTGGATGAGC ATGAGGAGGC GGAGGTGAAA GAAGACGAGG TGGAGGAGGA AGAACTTGAG
    GAAGAGATTG AGGAGGAAGA GGTTGCACTG GAACTTATGG CCCCGCAGGC CAAACGGCGG
    TGCAGAGAGG TCATCCCGAA CTGGGACTCC CTGCAGGATG GGGAAGACTC CCTAGATGAG
    ATGCTGCAAT ATCTGGGATA CTCCAGCCCA GAGTGTCTGC AACGTACTGG TGGACCTCTC
    AACATACCTG CCCCACCTCC AAACTGTGTG TCCGAGAAAC AGGAGAATGA TGTCATCAAT
    GCCATCCTGA AGCAGTGTGC CGAGGAGCAG GACTTCACAA TTCACCGTGG TAATGAGCTG
    AACATGAGGG CCGTCGTTCA GCAGGAGCCA AGCCCACAGA GACCACAGTC TGCCTTCTAC
    TACTGCAAAC TGCTCCTCAA CATACTAGGC ATGAACTCTT GGGAGAAGAG GAACAGTTTC
    CACCTGCTGA AAAAGAATGA AAAACTACTG AGAGAGCTGA AGAATCTGGA CTCCAGGCAG
    TGTCGTGAAA CACACAAAAT CGCGGTGTTT TACGTGGCAG AGGGACAAGA AGACAAGCAC
    TCCATTCTGT CCAACACAGC CGGGAGTCAG GCGTATGAAG ACTTTGTATC AGGCCTGGGC
    TGGGAGGTGA ACCTGACGAG TCACTGTGGC TTTATGGGTG GCCTGCAGCG GAACAAGAGC
    ACGGGTTTTA CCATGCCTTA CTTTGCCACC TCTACAGTGG AAGTGATGTT CCACGTCTCC
    ACACGCATGC CTCCAGACTC AGACGACTCG CTCACTAAAA AGTTGAGACA CCTGGGTAAT
    GATGAAGTGC ACATCGTGTG GTCTGAGCAC TCGCGGGACT ATCGGAGAGG CGTCATTCCT
    ACTGAGTTTG GAGACGTGCT CATCGTCATC TACCCTATGA AGAACCACAT GTACAGCATC
    CAGATCATCA AGAAACCTGA GGTGCCGTTC TTTGGTCCCT TGTTTGACGG AGCCATAGTG
    GATGGGACTA TATTGCCCAC AGTGGTGCGA GCGACAGCCA TCAACGCCAG CAGAGCTCTG
    AAATCCCTCA TCCCTCTATA CCAAAACTTC TATGAAGAGA GGGCCAGATA TCTGGAGACG
    ATAGTCCAAC ATCACTTGGA GCCCACGACA TTTGAGGACT ACGCCGCTCG TGTCTTTTGT
    CCTGCGCCTT TCCACTATCT TCCGTCTGAA GCAGGCTCAT GCCTCGAGAG TCAGCTGGCA
    GAAAGTCCAG CAATGCAGGT CGACGGGAGC GACTTAGCCT CGCCAATGTC ACCTCGTGCT
    AGCAAGAGCC GGATGTCCAT GAAGCTCCGC CGCTCCTCGG GCTCCGCCAA CAAGACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322986.1
    CDS624..8123
    Translation

    Target ORF information:

    RefSeq Version XM_021467311.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGGTG CACTTCCTGT CTCTGACAGT CCCTCTCACT CCCCCCACTT TACAGCCACC
    ACCACCACCA TTGAGTTCCC CCACAGTTCC CCAAACCCAC AGCAGCTCAC TGACCCACAG
    CAGCTCCCCG ACAGCCAGGC GTCCCTGGAG GTGGCCGAAA GTATCTATGA CCATCTCTGC
    CAAATGCCAG GCCAGCAGCC CCCCTTTATC CCCGATTGGT CCTGTCCAGC CGCGGCCTCG
    GCCAATCAAC CCGAGGGCGA AGCGGAGGAG GACGTCTTCA GTGCTTTTCG GGAGTTTTGT
    CACAGGGAGA ATGTGAAAAT GGAAGCAGGT GCAGATTTAA AGCAGGTGCA GTTTGAGGGC
    GATTGGGCTC TAGAATGGGA TAGAGAGATG GAGCAGAGCG AGAAGAGTTT GTATGAGTGC
    TTAGAGGAGT GTGATTGGGA TTTGAGTGTT AGTCCGCAGC CAGCAGAGGA CGGTAAAGGA
    GCACAGCTTC AGCGGCAGGA CGCCACCGAC AGGGCTAGCG AGAGTGAGGT TAGTCCACAG
    GCTTCGCATG GACCCATGTT GTCAAATATT GATCACAAGA AACGCCACAG CAGTGCGGTC
    CATGTCAGTT TCCGGCCCTC CACAGAGTCT GTGCTGTTCC ACAACCCTTT GGACCCCAAG
    GAGGCACACT GGAAAACCCG GCTTCGCCGT CTCGGGCACT TCAGCAGCCA CTCGGTTGGG
    GCGAAAGGTG TAGGGGAGGG GGTCACCTGT TGTGAAGTGG TGGAGCGGGA AAGAGGCTCC
    ATCAGCGGGG CTAGACGTGG ACGACTTGGT CGTCCGACTC TTCGGCCTCG AGGGTCCCGA
    TCTAGATCTC AGGAGTCAGG GTGCAGCCCC TCACGACAGC AGCAGGTGGC TCTGTTGGGT
    GGGGTGTATA AGTCTATGGT CCACGCTCTC TCCAAACCCA AGGGCCAGGC AGCATCGCCC
    CAGCGCCACA GCAAATCGCC CGCCACTGAG GCCCACCTGA AGGACCTGTA TGCTAGCGTT
    ATGGGCTATT TTGGAAGAAA AACATCAGCC AATAAAGACG AGCTGACGCC GAAGATGCGG
    CCCATGTCTA ACGATGCTGG CAGCAGCAAC CCCAATGTCA GTGACCTCAT GGACGAGTTC
    ATCCAGGAGA GACTGAGAGC CAGAGGCAAT TCTGGCATGA CGAGGCGCGG CAGTAGCCCA
    GGAAGTCTGG AGATCCCTAA AGATCTGCCA GAGCTGCTGA ACCGTCAGAA CGCCACACGT
    CCCGCAGACG ACCCCGGTGT GCCCTCTGAG TGGACCTCGC CTGCCAGTGC CAGTGGAAGT
    GACCTCATAA GCTCTGACAG CCAATCGGAT TCCTTTAACG CTTTTCAATA TGACAGCAAA
    TTCGAAAATT TCAGCTTCCC CCCTGAGACG TGTGCTTTGG CGTCTGCAGA TCAGGATAGT
    TTGAGTGGGA CAGGACAGAC AGCGGAGGAA CAGGAGCTTT CCAGTCTCAC AACACTTCAC
    ATCGACTCTG AGACCAGCAG CCTTAGTCAG CACGGCCTGT CTGCTGATAC TGTCACTATT
    ACTGGCTCTG AGAGCGCATC TCCAATGCAC TCTCTAGGAG GATCTCGATC GCAGACGCCA
    TCTCCTGCAA CCCTGACCGC TGAACACGTC CAGCATAAAG AGCTGCAGCT GGATGAGAAG
    ATGCACCATT CTGTTCTGCA GACCCCTGAT GACCTTGAAA CCAGCGAGTT TCCCACTGAG
    GACTGTAGTG TAATGGCTGG TGGCACTCTG ACTGGCTGGC ATGCTGATGT CGCAACAGTA
    ATGTGGAGGA GAATGCTTGG GATTCTGGGA GATGTCAACT ATATAAAGGA CCCTGAGATC
    CATGCGCAGG TTTTTGACTA CCTCTGCGAA CTCTGGCAGA ACCTGGCCAA GATCAGAGAC
    AATCTCGGGA TCTCCCTTGA CAACCAGTCG TCTCCGCCAC CACCAGATCT GATTCCACCT
    CTGCGGATTC TGACTCCATG GCTTTTCAAA GCGACTATGT TGGGCGAACG CTACAAGCAG
    GGAAAACTGC ACGCCTACAA GCTCATCTGT AAGATCATGA AGAGAAGACA GGACGTTTCC
    CCAAACTCTG ACTTCCTCAC GCACTTCTAC AACATCATGC ACTGCGGCCT GCTCCACCAA
    GACCAGGATA TTGTGAACAC CATCATTAAG CACTGCTCTC CACGCTTCTT CTCTATTGGA
    CTTCCAGGAG CCACAATGCT CATCCTGGAC TTCATCGTGG CAGCTAGTCG TGTCACTGCC
    TGCTCGTCCC TCAATGCTCC ACGGGTGGAG GCTCAGATCT TGTTGGGTTC CCTGGTTTGC
    CTCCCAAACT TGTACAATGA ACTTCCTGCA CTGCATCCTA CAACAGCCGA CATCATCATG
    ACCAAATTCA CTGATGTCAA GGAGCATATA ATCAAGCACA TCCTGACCTC TGCCAGAGAT
    GAGCCTTCTG CTCCAGCCAG GTGTGTGGCG CTCTGTAGTC TGGGTATTTG GCTGTGTGAA
    GAGCTGGTTC ATGGCACTCA GCATCCGCAG ATCAAAGAAG CGCTTAACGT CATCTGTGTC
    ACTCTCAAGT TCTCCAATAA AAACGTCGCC CTGGTGGCTT CAGACATTTT GCACCTGCTG
    ATAAACTACG TGGATGAACT GGAGAAATTT CCACCCAACA CTCCAAAGAA AATAGTGGAG
    GTTCTTATTG CAACCATCAC TTACCTTCTT CCAGCCACAG AGTCCTCTCC TCATGAGCTG
    GACAAGAGGC TGGTTGTTTC TCTACTGCTG TGCTTGCTGG ACTGGGTGAT GGCTCTACCT
    CCGAAAACAC TCCTCCAGCC GGTCCAAGGA GCCTCGGATA AGGAGAAAAC TGACAAGTCT
    GTCCTCAGCT GCATCTATAA AGTCCTCCAT GGTTGTGTCT ATGGGGCTCA GAGCTTCAGT
    AACCCCAGTT ACTTCCCCAT TCACCTGTCA GACTTAAGCA GCCCAGACTA CGACCCCTTC
    TTACTACTAG AAAACCTTAA GGAGCCGGAG CCACTCCATT CACCTGACTC TGAACGCTCC
    TCCAAACTGC AACCAGTAAC TGAAGTGAGG AGCCGCATTC AGCAAGGCCT TGTTTCAGTT
    GCGGCACGCA CTGTCATATC TCACCTAGTC AACCATCTGG GTCACTATCC CATGAGTGGT
    GGTCCGGCCA CTCTGACGAG CCAGGTGTGC GAGAACCAGG ACAACCCATA CAGTGAGAGC
    GCAGACCTGA CCCCTGAACT CTTTGACGCC CCCAATTTGC AGTTCTTCGT GCTTAATGGC
    ACCACCCTGC TGTCCTACTT ACAAATTCGG GCTGAAGATG GCCTGCCCGG TGGTGGGATG
    TCAGCTGGCC TCACCACCAC CAATGCTTGT GTTCGGGTGA TTGTGAGGGA CATTTCTGGT
    AAACACTCCT GGGACTCAGC TGTTCTGTAT GGTCCACCTC ATTGCATCAC TCCTAGTCAA
    ACTTTGCACC TTTACACGTC CTCTAATCCG AGAGGGGAAC ATAATAGAGA TGAGGACAGT
    TTAGAGAGGG GCAGCCAAGA AGAACCAGAG GATGGAGCTT TTCAGGAAAG CGAGGAGGAC
    TTGGATGAGC ATGAGGAGGC GGAGGTGAAA GAAGACGAGG TGGAGGAGGA AGAACTTGAG
    GAAGAGATTG AGGAGGAAGA GGTTGCACTG GAACTTATGG CCCCGCAGGC CAAACGGCGG
    TGCAGAGAGG TCATCCCGAA CTGGGACTCC CTGCAGGATG GGGAAGACTC CCTAGATGAG
    ATGCTGCAAT ATCTGGGATA CTCCAGCCCA GAGTGTCTGC AACGTACTGG TGGACCTCTC
    AACATACCTG CCCCACCTCC AAACTGTGTG TCCGAGAAAC AGGAGAATGA TGTCATCAAT
    GCCATCCTGA AGCAGTGTGC CGAGGAGCAG GACTTCACAA TTCACCGTGG TAATGAGCTG
    AACATGAGGG CCGTCGTTCA GCAGGAGCCA AGCCCACAGA GACCACAGTC TGCCTTCTAC
    TACTGCAAAC TGCTCCTCAA CATACTAGGC ATGAACTCTT GGGAGAAGAG GAACAGTTTC
    CACCTGCTGA AAAAGAATGA AAAACTACTG AGAGAGCTGA AGAATCTGGA CTCCAGGCAG
    TGTCGTGAAA CACACAAAAT CGCGGTGTTT TACGTGGCAG AGGGACAAGA AGACAAGCAC
    TCCATTCTGT CCAACACAGC CGGGAGTCAG GCGTATGAAG ACTTTGTATC AGGCCTGGGC
    TGGGAGGTGA ACCTGACGAG TCACTGTGGC TTTATGGGTG GCCTGCAGCG GAACAAGAGC
    ACGGGTTTTA CCATGCCTTA CTTTGCCACC TCTACAGTGG AAGTGATGTT CCACGTCTCC
    ACACGCATGC CTCCAGACTC AGACGACTCG CTCACTAAAA AGTTGAGACA CCTGGGTAAT
    GATGAAGTGC ACATCGTGTG GTCTGAGCAC TCGCGGGACT ATCGGAGAGG CGTCATTCCT
    ACTGAGTTTG GAGACGTGCT CATCGTCATC TACCCTATGA AGAACCACAT GTACAGCATC
    CAGATCATCA AGAAACCTGA GGTGCCGTTC TTTGGTCCCT TGTTTGACGG AGCCATAGTG
    GATGGGACTA TATTGCCCAC AGTGGTGCGA GCGACAGCCA TCAACGCCAG CAGAGCTCTG
    AAATCCCTCA TCCCTCTATA CCAAAACTTC TATGAAGAGA GGGCCAGATA TCTGGAGACG
    ATAGTCCAAC ATCACTTGGA GCCCACGACA TTTGAGGACT ACGCCGCTCG TGTCTTTTGT
    CCTGCGCCTT TCCACTATCT TCCGTCTGAA GCAGGCTCAT GCCTCGAGAG TCAGCTGGCA
    GAAAGTCCAG CAATGCAGGT CGACGGGAGC GACTTAGCCT CGCCAATGTC ACCTCGTGCT
    AGCAAGAGCC GGATGTCCAT GAAGCTCCGC CGCTCCTCGG GCTCCGCCAA CAAGACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149650
    Clone ID Related Accession (Same CDS sequence) XM_021467312.1
    Accession Version XM_021467312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGA CCTGGAGGAG
    CCTCCTATGA TCCAGCGTGG GCCACGGAAC CGCCACTGCT CTCAGAGCGA GGAGACGCCG
    ACGTCTGAAG TTTTTTCGGC CTCCGGTGAA CTGGAGCAGC CTCCGCTTCC CCGCAGTAGC
    AGTGCCTCCG ACATCATGGA GCCCTTCATC GCAGAACGGG CCAAAGGTGC ACTTCCTGTC
    TCTGACAGTC CCTCTCACTC CCCCCACTTT ACAGCCACCA CCACCACCAT TGAGTTCCCC
    CACAGTTCCC CAAACCCACA GCAGCTCACT GACCCACAGC AGCTCCCCGA CAGCCAGGCG
    TCCCTGGAGG TGGCCGAAAG TATCTATGAC CATCTCTGCC AAATGCCAGG CCAGCAGCCC
    CCCTTTATCC CCGATTGGTC CTGTCCAGCC GCGGCCTCGG CCAATCAACC CGAGGGCGAA
    GCGGAGGAGG ACGTCTTCAG TGCTTTTCGG GAGTTTTGTC ACAGGGAGAA TGTGAAAATG
    GAAGCAGGTG CAGATTTAAA GCAGGTGCAG TTTGAGGGCG ATTGGGCTCT AGAATGGGAT
    AGAGAGATGG AGCAGAGCGA GAAGAGTTTG TATGAGTGCT TAGAGGAGTG TGATTGGGAT
    TTGAGTGTTA GTCCGCAGCC AGCAGAGGAC GGTAAAGGAG CACAGCTTCA GCGGCAGGAC
    GCCACCGACA GGGCTAGCGA GAGTGAGGTT AGTCCACAGG CTTCGCATGG ACCCATGTTG
    TCAAATATTG ATCACAAGAA ACGCCACAGC AGTGCGGTCC ATGTCAGTTT CCGGCCCTCC
    ACAGAGTCTG TGCTGTTCCA CAACCCTTTG GACCCCAAGG AGGCACACTG GAAAACCCGG
    CTTCGCCGTC TCGGGCACTT CAGCAGCCAC TCGGTTGGGG CGAAAGGTGT AGGGGAGGGG
    GTCACCTGTT GTGAAGTGGT GGAGCGGGAA AGAGGCTCCA TCAGCGGGGC TAGACGTGGA
    CGACTTGGTC GTCCGACTCT TCGGCCTCGA GGGTCCCGAT CTAGATCTCA GGAGTCAGGG
    TGCAGCCCCT CACGACAGCA GCAGGTGGCT CTGTTGGGTG GGGTGTATAA GTCTATGGTC
    CACGCTCTCT CCAAACCCAA GGGCCAGGCA GCATCGCCCC AGCGCCACAG CAAATCGCCC
    GCCACTGAGG CCCACCTGAA GGACCTGTAT GCTAGCGTTA TGGGCTATTT TGGAAGAAAA
    ACATCAGCCA ATAAAGACGA GCTGACGCCG AAGATGCGGC CCATGTCTAA CGATGCTGGC
    AGCAGCAACC CCAATGTCAG TGACCTCATG GACGAGTTCA TCCAGGAGAG ACTGAGAGCC
    AGAGGCAATT CTGGCATGAC GAGGCGCGGC AGTAGCCCAG GAAGTCTGGA GATCCCTAAA
    GATCTGCCAG AGCTGCTGAA CCGTCAGAAC GCCACACGTC CCGCAGACGA CCCCGGTGTG
    CCCTCTGAGT GGACCTCGCC TGCCAGTGCC AGTGGAAGTG ACCTCATAAG CTCTGACAGC
    CAATCGGATT CCTTTAACGC TTTTCAATAT GACAGCAAAT TCGAAAATTT CAGCTTCCCC
    CCTGAGACGT GTGCTTTGGC GTCTGCAGAT CAGGATAGTT TGAGTGGGAC AGGACAGACA
    GCGGAGGAAC AGGAGCTTTC CAGTCTCACA ACACTTCACA TCGACTCTGA GACCAGCAGC
    CTTAGTCAGC ACGGCCTGTC TGCTGATACT GTCACTATTA CTGGCTCTGA GAGCGCATCT
    CCAATGCACT CTCTAGGAGG ATCTCGATCG CAGACGCCAT CTCCTGCAAC CCTGACCGCT
    GAACACGTCC AGCATAAAGA GCTGCAGCTG GATGAGAAGA TGCACCATTC TGTTCTGCAG
    ACCCCTGATG ACCTTGAAAC CAGCGAGTTT CCCACTGAGG ACTGTAGTGT AATGGCTGGT
    GGCACTCTGA CTGGCTGGCA TGCTGATGTC GCAACAGTAA TGTGGAGGAG AATGCTTGGG
    ATTCTGGGAG ATGTCAACTA TATAAAGGAC CCTGAGATCC ATGCGCAGGT TTTTGACTAC
    CTCTGCGAAC TCTGGCAGAA CCTGGCCAAG ATCAGAGACA ATCTCGGGAT CTCCCTTGAC
    AACCAGTCGT CTCCGCCACC ACCAGATCTG ATTCCACCTC TGCGGATTCT GACTCCATGG
    CTTTTCAAAG CGACTATGTT GGGCGAACGC TACAAGCAGG GAAAACTGCA CGCCTACAAG
    CTCATCTGTA AGATCATGAA GAGAAGACAG GACGTTTCCC CAAACTCTGA CTTCCTCACG
    CACTTCTACA ACATCATGCA CTGCGGCCTG CTCCACCAAG ACCAGGATAT TGTGAACACC
    ATCATTAAGC ACTGCTCTCC ACGCTTCTTC TCTATTGGAC TTCCAGGAGC CACAATGCTC
    ATCCTGGACT TCATCGTGGC AGCTAGTCGT GTCACTGCCT GCTCGTCCCT CAATGCTCCA
    CGGGTGGAGG CTCAGATCTT GTTGGGTTCC CTGGTTTGCC TCCCAAACTT GTACAATGAA
    CTTCCTGCAC TGCATCCTAC AACAGCCGAC ATCATCATGA CCAAATTCAC TGATGTCAAG
    GAGCATATAA TCAAGCACAT CCTGACCTCT GCCAGAGATG AGCCTTCTGC TCCAGCCAGG
    TGTGTGGCGC TCTGTAGTCT GGGTATTTGG CTGTGTGAAG AGCTGGTTCA TGGCACTCAG
    CATCCGCAGA TCAAAGAAGC GCTTAACGTC ATCTGTGTCA CTCTCAAGTT CTCCAATAAA
    AACGTCGCCC TGGTGGCTTC AGACATTTTG CACCTGCTGA TAAACTACGT GGATGAACTG
    GAGAAATTTC CACCCAACAC TCCAAAGAAA ATAGTGGAGG TTCTTATTGC AACCATCACT
    TACCTTCTTC CAGCCACAGA GTCCTCTCCT CATGAGCTGG ACAAGAGGCT GGTTGTTTCT
    CTACTGCTGT GCTTGCTGGA CTGGGTGATG GCTCTACCTC CGAAAACACT CCTCCAGCCG
    GTCCAAGGAG CCTCGGATAA GGAGAAAACT GACAAGTCTG TCCTCAGCTG CATCTATAAA
    GTCCTCCATG GTTGTGTCTA TGGGGCTCAG AGCTTCAGTA ACCCCAGTTA CTTCCCCATT
    CACCTGTCAG ACTTAAGCAG CCCAGACTAC GACCCCTTCT TACTACTAGA AAACCTTAAG
    GAGCCGGAGC CACTCCATTC ACCTGACTCT GAACGCTCCT CCAAACTGCA ACCAGTAACT
    GAAGTGAGGA GCCGCATTCA GCAAGGCCTT GTTTCAGTTG CGGCACGCAC TGTCATATCT
    CACCTAGTCA ACCATCTGGG TCACTATCCC ATGAGTGGTG GTCCGGCCAC TCTGACGAGC
    CAGGTGTGCG AGAACCAGGA CAACCCATAC AGTGAGAGCG CAGACCTGAC CCCTGAACTC
    TTTGACGCCC CCAATTTGCA GTTCTTCGTG CTTAATGGCA CCACCCTGCT GTCCTACTTA
    CAAATTCGGG CTGAAGATGG CCTGCCCGGT GGTGGGATGT CAGCTGGCCT CACCACCACC
    AATGCTTGTG TTCGGGTGAT TGTGAGGGAC ATTTCTGGTA AACACTCCTG GGACTCAGCT
    GTTCTGTATG GTCCACCTCA TTGCATCACT CCTAGTCAAA CTTTGCACCT TTACACGTCC
    TCTAATCCGA GAGGGGAACA TAATAGAGAT GAGGACAGTT TAGAGAGGGG CAGCCAAGAA
    GAACCAGAGG ATGGAGCTTT TCAGGAAAGC GAGGAGGACT TGGATGAGCA TGAGGAGGCG
    GAGGTGAAAG AAGACGAGGT GGAGGAGGAA GAACTTGAGG AAGAGATTGA GGAGGAAGAG
    GTTGCACTGG AACTTATGGC CCCGCAGGCC AAACGGCGGT GCAGAGAGGT CATCCCGAAC
    TGGGACTCCC TGCAGGATGG GGAAGACTCC CTAGATGAGA TGCTGCAATA TCTGGGATAC
    TCCAGCCCAG AGTGTCTGCA ACGTACTGGT GGACCTCTCA ACATACCTGC CCCACCTCCA
    AACTGTGTGT CCGAGAAACA GGAGAATGAT GTCATCAATG CCATCCTGAA GCAGTGTGCC
    GAGGAGCAGG ACTTCACAAT TCACCGTGGT AATGAGCTGA ACATGAGGGC CGTCGTTCAG
    CAGGAGCCAA GCCCACAGAG ACCACAGTCT GCCTTCTACT ACTGCAAACT GCTCCTCAAC
    ATACTAGGCA TGAACTCTTG GGAGAAGAGG AACAGTTTCC ACCTGCTGAA AAAGAATGAA
    AAACTACTGA GAGAGCTGAA GAATCTGGAC TCCAGGCAGT GTCGTGAAAC ACACAAAATC
    GCGGTGTTTT ACGTGGCAGA GGGACAAGAA GACAAGCACT CCATTCTGTC CAACACAGCC
    GGGAGTCAGG CGTATGAAGA CTTTGTATCA GGCCTGGGCT GGGAGGTGAA CCTGACGAGT
    CACTGTGGCT TTATGGGTGG CCTGCAGCGG AACAAGAGCA CGGGTTTTAC CATGCCTTAC
    TTTGCCACCT CTACAGTGGA AGTGATGTTC CACGTCTCCA CACGCATGCC TCCAGACTCA
    GACGACTCGC TCACTAAAAA GTTGAGACAC CTGGGTAATG ATGAAGTGCA CATCGTGTGG
    TCTGAGCACT CGCGGGACTA TCGGAGAGGC GTCATTCCTA CTGAGTTTGG AGACGTGCTC
    ATCGTCATCT ACCCTATGAA GAACCACATG TACAGCATCC AGATCATCAA GAAACCTGAG
    GTGCCGTTCT TTGGTCCCTT GTTTGACGGA GCCATAGTGG ATGGGACTAT ATTGCCCACA
    GTGGTGCGAG CGACAGCCAT CAACGCCAGC AGAGCTCTGA AATCCCTCAT CCCTCTATAC
    CAAAACTTCT ATGAAGAGAG GGCCAGATAT CTGGAGACGA TAGTCCAACA TCACTTGGAG
    CCCACGACAT TTGAGGACTA CGCCGCTCGT GTCTTTTGTC CTGCGCCTTT CCACTATCTT
    CCGTCTGAAG CAGAGAGAAA TGCTGTGATT TTTCTGCCCC TCCCTAAACC TCCACTAGAA
    AACATTGGGC TCACCAAGGT AAAGGGCAAA GGCTTGAAAC GGAAGGTCTT TCACGCTAGC
    TGTGAATGGC TCATGCCTCG AGAGTCAGCT GGCAGAAAGT CCAGCAATGC AGGTCGACGG
    GAGCGACTTA GCCTCGCCAA TGTCACCTCG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322987.1
    CDS652..8067
    Translation

    Target ORF information:

    RefSeq Version XM_021467312.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGA CCTGGAGGAG
    CCTCCTATGA TCCAGCGTGG GCCACGGAAC CGCCACTGCT CTCAGAGCGA GGAGACGCCG
    ACGTCTGAAG TTTTTTCGGC CTCCGGTGAA CTGGAGCAGC CTCCGCTTCC CCGCAGTAGC
    AGTGCCTCCG ACATCATGGA GCCCTTCATC GCAGAACGGG CCAAAGGTGC ACTTCCTGTC
    TCTGACAGTC CCTCTCACTC CCCCCACTTT ACAGCCACCA CCACCACCAT TGAGTTCCCC
    CACAGTTCCC CAAACCCACA GCAGCTCACT GACCCACAGC AGCTCCCCGA CAGCCAGGCG
    TCCCTGGAGG TGGCCGAAAG TATCTATGAC CATCTCTGCC AAATGCCAGG CCAGCAGCCC
    CCCTTTATCC CCGATTGGTC CTGTCCAGCC GCGGCCTCGG CCAATCAACC CGAGGGCGAA
    GCGGAGGAGG ACGTCTTCAG TGCTTTTCGG GAGTTTTGTC ACAGGGAGAA TGTGAAAATG
    GAAGCAGGTG CAGATTTAAA GCAGGTGCAG TTTGAGGGCG ATTGGGCTCT AGAATGGGAT
    AGAGAGATGG AGCAGAGCGA GAAGAGTTTG TATGAGTGCT TAGAGGAGTG TGATTGGGAT
    TTGAGTGTTA GTCCGCAGCC AGCAGAGGAC GGTAAAGGAG CACAGCTTCA GCGGCAGGAC
    GCCACCGACA GGGCTAGCGA GAGTGAGGTT AGTCCACAGG CTTCGCATGG ACCCATGTTG
    TCAAATATTG ATCACAAGAA ACGCCACAGC AGTGCGGTCC ATGTCAGTTT CCGGCCCTCC
    ACAGAGTCTG TGCTGTTCCA CAACCCTTTG GACCCCAAGG AGGCACACTG GAAAACCCGG
    CTTCGCCGTC TCGGGCACTT CAGCAGCCAC TCGGTTGGGG CGAAAGGTGT AGGGGAGGGG
    GTCACCTGTT GTGAAGTGGT GGAGCGGGAA AGAGGCTCCA TCAGCGGGGC TAGACGTGGA
    CGACTTGGTC GTCCGACTCT TCGGCCTCGA GGGTCCCGAT CTAGATCTCA GGAGTCAGGG
    TGCAGCCCCT CACGACAGCA GCAGGTGGCT CTGTTGGGTG GGGTGTATAA GTCTATGGTC
    CACGCTCTCT CCAAACCCAA GGGCCAGGCA GCATCGCCCC AGCGCCACAG CAAATCGCCC
    GCCACTGAGG CCCACCTGAA GGACCTGTAT GCTAGCGTTA TGGGCTATTT TGGAAGAAAA
    ACATCAGCCA ATAAAGACGA GCTGACGCCG AAGATGCGGC CCATGTCTAA CGATGCTGGC
    AGCAGCAACC CCAATGTCAG TGACCTCATG GACGAGTTCA TCCAGGAGAG ACTGAGAGCC
    AGAGGCAATT CTGGCATGAC GAGGCGCGGC AGTAGCCCAG GAAGTCTGGA GATCCCTAAA
    GATCTGCCAG AGCTGCTGAA CCGTCAGAAC GCCACACGTC CCGCAGACGA CCCCGGTGTG
    CCCTCTGAGT GGACCTCGCC TGCCAGTGCC AGTGGAAGTG ACCTCATAAG CTCTGACAGC
    CAATCGGATT CCTTTAACGC TTTTCAATAT GACAGCAAAT TCGAAAATTT CAGCTTCCCC
    CCTGAGACGT GTGCTTTGGC GTCTGCAGAT CAGGATAGTT TGAGTGGGAC AGGACAGACA
    GCGGAGGAAC AGGAGCTTTC CAGTCTCACA ACACTTCACA TCGACTCTGA GACCAGCAGC
    CTTAGTCAGC ACGGCCTGTC TGCTGATACT GTCACTATTA CTGGCTCTGA GAGCGCATCT
    CCAATGCACT CTCTAGGAGG ATCTCGATCG CAGACGCCAT CTCCTGCAAC CCTGACCGCT
    GAACACGTCC AGCATAAAGA GCTGCAGCTG GATGAGAAGA TGCACCATTC TGTTCTGCAG
    ACCCCTGATG ACCTTGAAAC CAGCGAGTTT CCCACTGAGG ACTGTAGTGT AATGGCTGGT
    GGCACTCTGA CTGGCTGGCA TGCTGATGTC GCAACAGTAA TGTGGAGGAG AATGCTTGGG
    ATTCTGGGAG ATGTCAACTA TATAAAGGAC CCTGAGATCC ATGCGCAGGT TTTTGACTAC
    CTCTGCGAAC TCTGGCAGAA CCTGGCCAAG ATCAGAGACA ATCTCGGGAT CTCCCTTGAC
    AACCAGTCGT CTCCGCCACC ACCAGATCTG ATTCCACCTC TGCGGATTCT GACTCCATGG
    CTTTTCAAAG CGACTATGTT GGGCGAACGC TACAAGCAGG GAAAACTGCA CGCCTACAAG
    CTCATCTGTA AGATCATGAA GAGAAGACAG GACGTTTCCC CAAACTCTGA CTTCCTCACG
    CACTTCTACA ACATCATGCA CTGCGGCCTG CTCCACCAAG ACCAGGATAT TGTGAACACC
    ATCATTAAGC ACTGCTCTCC ACGCTTCTTC TCTATTGGAC TTCCAGGAGC CACAATGCTC
    ATCCTGGACT TCATCGTGGC AGCTAGTCGT GTCACTGCCT GCTCGTCCCT CAATGCTCCA
    CGGGTGGAGG CTCAGATCTT GTTGGGTTCC CTGGTTTGCC TCCCAAACTT GTACAATGAA
    CTTCCTGCAC TGCATCCTAC AACAGCCGAC ATCATCATGA CCAAATTCAC TGATGTCAAG
    GAGCATATAA TCAAGCACAT CCTGACCTCT GCCAGAGATG AGCCTTCTGC TCCAGCCAGG
    TGTGTGGCGC TCTGTAGTCT GGGTATTTGG CTGTGTGAAG AGCTGGTTCA TGGCACTCAG
    CATCCGCAGA TCAAAGAAGC GCTTAACGTC ATCTGTGTCA CTCTCAAGTT CTCCAATAAA
    AACGTCGCCC TGGTGGCTTC AGACATTTTG CACCTGCTGA TAAACTACGT GGATGAACTG
    GAGAAATTTC CACCCAACAC TCCAAAGAAA ATAGTGGAGG TTCTTATTGC AACCATCACT
    TACCTTCTTC CAGCCACAGA GTCCTCTCCT CATGAGCTGG ACAAGAGGCT GGTTGTTTCT
    CTACTGCTGT GCTTGCTGGA CTGGGTGATG GCTCTACCTC CGAAAACACT CCTCCAGCCG
    GTCCAAGGAG CCTCGGATAA GGAGAAAACT GACAAGTCTG TCCTCAGCTG CATCTATAAA
    GTCCTCCATG GTTGTGTCTA TGGGGCTCAG AGCTTCAGTA ACCCCAGTTA CTTCCCCATT
    CACCTGTCAG ACTTAAGCAG CCCAGACTAC GACCCCTTCT TACTACTAGA AAACCTTAAG
    GAGCCGGAGC CACTCCATTC ACCTGACTCT GAACGCTCCT CCAAACTGCA ACCAGTAACT
    GAAGTGAGGA GCCGCATTCA GCAAGGCCTT GTTTCAGTTG CGGCACGCAC TGTCATATCT
    CACCTAGTCA ACCATCTGGG TCACTATCCC ATGAGTGGTG GTCCGGCCAC TCTGACGAGC
    CAGGTGTGCG AGAACCAGGA CAACCCATAC AGTGAGAGCG CAGACCTGAC CCCTGAACTC
    TTTGACGCCC CCAATTTGCA GTTCTTCGTG CTTAATGGCA CCACCCTGCT GTCCTACTTA
    CAAATTCGGG CTGAAGATGG CCTGCCCGGT GGTGGGATGT CAGCTGGCCT CACCACCACC
    AATGCTTGTG TTCGGGTGAT TGTGAGGGAC ATTTCTGGTA AACACTCCTG GGACTCAGCT
    GTTCTGTATG GTCCACCTCA TTGCATCACT CCTAGTCAAA CTTTGCACCT TTACACGTCC
    TCTAATCCGA GAGGGGAACA TAATAGAGAT GAGGACAGTT TAGAGAGGGG CAGCCAAGAA
    GAACCAGAGG ATGGAGCTTT TCAGGAAAGC GAGGAGGACT TGGATGAGCA TGAGGAGGCG
    GAGGTGAAAG AAGACGAGGT GGAGGAGGAA GAACTTGAGG AAGAGATTGA GGAGGAAGAG
    GTTGCACTGG AACTTATGGC CCCGCAGGCC AAACGGCGGT GCAGAGAGGT CATCCCGAAC
    TGGGACTCCC TGCAGGATGG GGAAGACTCC CTAGATGAGA TGCTGCAATA TCTGGGATAC
    TCCAGCCCAG AGTGTCTGCA ACGTACTGGT GGACCTCTCA ACATACCTGC CCCACCTCCA
    AACTGTGTGT CCGAGAAACA GGAGAATGAT GTCATCAATG CCATCCTGAA GCAGTGTGCC
    GAGGAGCAGG ACTTCACAAT TCACCGTGGT AATGAGCTGA ACATGAGGGC CGTCGTTCAG
    CAGGAGCCAA GCCCACAGAG ACCACAGTCT GCCTTCTACT ACTGCAAACT GCTCCTCAAC
    ATACTAGGCA TGAACTCTTG GGAGAAGAGG AACAGTTTCC ACCTGCTGAA AAAGAATGAA
    AAACTACTGA GAGAGCTGAA GAATCTGGAC TCCAGGCAGT GTCGTGAAAC ACACAAAATC
    GCGGTGTTTT ACGTGGCAGA GGGACAAGAA GACAAGCACT CCATTCTGTC CAACACAGCC
    GGGAGTCAGG CGTATGAAGA CTTTGTATCA GGCCTGGGCT GGGAGGTGAA CCTGACGAGT
    CACTGTGGCT TTATGGGTGG CCTGCAGCGG AACAAGAGCA CGGGTTTTAC CATGCCTTAC
    TTTGCCACCT CTACAGTGGA AGTGATGTTC CACGTCTCCA CACGCATGCC TCCAGACTCA
    GACGACTCGC TCACTAAAAA GTTGAGACAC CTGGGTAATG ATGAAGTGCA CATCGTGTGG
    TCTGAGCACT CGCGGGACTA TCGGAGAGGC GTCATTCCTA CTGAGTTTGG AGACGTGCTC
    ATCGTCATCT ACCCTATGAA GAACCACATG TACAGCATCC AGATCATCAA GAAACCTGAG
    GTGCCGTTCT TTGGTCCCTT GTTTGACGGA GCCATAGTGG ATGGGACTAT ATTGCCCACA
    GTGGTGCGAG CGACAGCCAT CAACGCCAGC AGAGCTCTGA AATCCCTCAT CCCTCTATAC
    CAAAACTTCT ATGAAGAGAG GGCCAGATAT CTGGAGACGA TAGTCCAACA TCACTTGGAG
    CCCACGACAT TTGAGGACTA CGCCGCTCGT GTCTTTTGTC CTGCGCCTTT CCACTATCTT
    CCGTCTGAAG CAGAGAGAAA TGCTGTGATT TTTCTGCCCC TCCCTAAACC TCCACTAGAA
    AACATTGGGC TCACCAAGGT AAAGGGCAAA GGCTTGAAAC GGAAGGTCTT TCACGCTAGC
    TGTGAATGGC TCATGCCTCG AGAGTCAGCT GGCAGAAAGT CCAGCAATGC AGGTCGACGG
    GAGCGACTTA GCCTCGCCAA TGTCACCTCG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149651
    Clone ID Related Accession (Same CDS sequence) XM_021467313.1
    Accession Version XM_021467313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGGTG CACTTCCTGT CTCTGACAGT CCCTCTCACT CCCCCCACTT TACAGCCACC
    ACCACCACCA TTGAGTTCCC CCACAGTTCC CCAAACCCAC AGCAGCTCAC TGACCCACAG
    CAGCTCCCCG ACAGCCAGGC GTCCCTGGAG GTGGCCGAAA GTATCTATGA CCATCTCTGC
    CAAATGCCAG GCCAGCAGCC CCCCTTTATC CCCGATTGGT CCTGTCCAGC CGCGGCCTCG
    GCCAATCAAC CCGAGGGCGA AGCGGAGGAG GACGTCTTCA GTGCTTTTCG GGAGTTTTGT
    CACAGGGAGA ATGTGAAAAT GGAAGCAGGT GCAGATTTAA AGCAGGTGCA GTTTGAGGGC
    GATTGGGCTC TAGAATGGGA TAGAGAGATG GAGCAGAGCG AGAAGAGTTT GTATGAGTGC
    TTAGAGGAGT GTGATTGGGA TTTGAGTGTT AGTCCGCAGC CAGCAGAGGA CGGTAAAGGA
    GCACAGCTTC AGCGGCAGGA CGCCACCGAC AGGGCTAGCG AGAGTGAGGT TAGTCCACAG
    GCTTCGCATG GACCCATGTT GTCAAATATT GATCACAAGA AACGCCACAG CAGTGCGGTC
    CATGTCAGTT TCCGGCCCTC CACAGAGTCT GTGCTGTTCC ACAACCCTTT GGACCCCAAG
    GAGGCACACT GGAAAACCCG GCTTCGCCGT CTCGGGCACT TCAGCAGCCA CTCGGTTGGG
    GCGAAAGGTG TAGGGGAGGG GGTCACCTGT TGTGAAGTGG TGGAGCGGGA AAGAGGCTCC
    ATCAGCGGGG CTAGACGTGG ACGACTTGGT CGTCCGACTC TTCGGCCTCG AGGGTCCCGA
    TCTAGATCTC AGGAGTCAGG GTGCAGCCCC TCACGACAGC AGCAGGTGGC TCTGTTGGGT
    GGGGTGTATA AGTCTATGGT CCACGCTCTC TCCAAACCCA AGGGCCAGGC AGCATCGCCC
    CAGCGCCACA GCAAATCGCC CGCCACTGAG GCCCACCTGA AGGACCTGTA TGCTAGCGTT
    ATGGGCTATT TTGGAAGAAA AACATCAGCC AATAAAGACG AGCTGACGCC GAAGATGCGG
    CCCATGTCTA ACGATGCTGG CAGCAGCAAC CCCAATGTCA GTGACCTCAT GGACGAGTTC
    ATCCAGGAGA GACTGAGAGC CAGAGGCAAT TCTGGCATGA CGAGGCGCGG CAGTAGCCCA
    GGAAGTCTGG AGATCCCTAA AGATCTGCCA GAGCTGCTGA ACCGTCAGAA CGCCACACGT
    CCCGCAGACG ACCCCGGTGT GCCCTCTGAG TGGACCTCGC CTGCCAGTGC CAGTGGAAGT
    GACCTCATAA GCTCTGACAG CCAATCGGAT TCCTTTAACG CTTTTCAATA TGACAGCAAA
    TTCGAAAATT TCAGCTTCCC CCCTGAGACG TGTGCTTTGG CGTCTGCAGA TCAGGATAGT
    TTGAGTGGGA CAGGACAGAC AGCGGAGGAA CAGGAGCTTT CCAGTCTCAC AACACTTCAC
    ATCGACTCTG AGACCAGCAG CCTTAGTCAG CACGGCCTGT CTGCTGATAC TGTCACTATT
    ACTGGCTCTG AGAGCGCATC TCCAATGCAC TCTCTAGGAG GATCTCGATC GCAGACGCCA
    TCTCCTGCAA CCCTGACCGC TGAACACGTC CAGCATAAAG AGCTGCAGCT GGATGAGAAG
    ATGCACCATT CTGTTCTGCA GACCCCTGAT GACCTTGAAA CCAGCGAGTT TCCCACTGAG
    GACTGTAGTG TAATGGCTGG TGGCACTCTG ACTGGCTGGC ATGCTGATGT CGCAACAGTA
    ATGTGGAGGA GAATGCTTGG GATTCTGGGA GATGTCAACT ATATAAAGGA CCCTGAGATC
    CATGCGCAGG TTTTTGACTA CCTCTGCGAA CTCTGGCAGA ACCTGGCCAA GATCAGAGAC
    AATCTCGGGA TCTCCCTTGA CAACCAGTCG TCTCCGCCAC CACCAGATCT GATTCCACCT
    CTGCGGATTC TGACTCCATG GCTTTTCAAA GCGACTATGT TGGGCGAACG CTACAAGCAG
    GGAAAACTGC ACGCCTACAA GCTCATCTGT AAGATCATGA AGAGAAGACA GGACGTTTCC
    CCAAACTCTG ACTTCCTCAC GCACTTCTAC AACATCATGC ACTGCGGCCT GCTCCACCAA
    GACCAGGATA TTGTGAACAC CATCATTAAG CACTGCTCTC CACGCTTCTT CTCTATTGGA
    CTTCCAGGAG CCACAATGCT CATCCTGGAC TTCATCGTGG CAGCTAGTCG TGTCACTGCC
    TGCTCGTCCC TCAATGCTCC ACGGGTGGAG GCTCAGATCT TGTTGGGTTC CCTGGTTTGC
    CTCCCAAACT TGTACAATGA ACTTCCTGCA CTGCATCCTA CAACAGCCGA CATCATCATG
    ACCAAATTCA CTGATGTCAA GGAGCATATA ATCAAGCACA TCCTGACCTC TGCCAGAGAT
    GAGCCTTCTG CTCCAGCCAG GTGTGTGGCG CTCTGTAGTC TGGGTATTTG GCTGTGTGAA
    GAGCTGGTTC ATGGCACTCA GCATCCGCAG ATCAAAGAAG CGCTTAACGT CATCTGTGTC
    ACTCTCAAGT TCTCCAATAA AAACGTCGCC CTGGTGGCTT CAGACATTTT GCACCTGCTG
    ATAAACTACG TGGATGAACT GGAGAAATTT CCACCCAACA CTCCAAAGAA AATAGTGGAG
    GTTCTTATTG CAACCATCAC TTACCTTCTT CCAGCCACAG AGTCCTCTCC TCATGAGCTG
    GACAAGAGGC TGGTTGTTTC TCTACTGCTG TGCTTGCTGG ACTGGGTGAT GGCTCTACCT
    CCGAAAACAC TCCTCCAGCC GGTCCAAGGA GCCTCGGATA AGGAGAAAAC TGACAAGTCT
    GTCCTCAGCT GCATCTATAA AGTCCTCCAT GGTTGTGTCT ATGGGGCTCA GAGCTTCAGT
    AACCCCAGTT ACTTCCCCAT TCACCTGTCA GACTTAAGCA GCCCAGACTA CGACCCCTTC
    TTACTACTAG AAAACCTTAA GGAGCCGGAG CCACTCCATT CACCTGACTC TGAACGCTCC
    TCCAAACTGC AACCAGTAAC TGAAGTGAGG AGCCGCATTC AGCAAGGCCT TGTTTCAGTT
    GCGGCACGCA CTGTCATATC TCACCTAGTC AACCATCTGG GTCACTATCC CATGAGTGGT
    GGTCCGGCCA CTCTGACGAG CCAGGTGTGC GAGAACCAGG ACAACCCATA CAGTGAGAGC
    GCAGACCTGA CCCCTGAACT CTTTGACGCC CCCAATTTGC AGTTCTTCGT GCTTAATGGC
    ACCACCCTGC TGTCCTACTT ACAAATTCGG GCTGAAGATG GCCTGCCCGG TGGTGGGATG
    TCAGCTGGCC TCACCACCAC CAATGCTTGT GTTCGGGTGA TTGTGAGGGA CATTTCTGGT
    AAACACTCCT GGGACTCAGC TGTTCTGTAT GGTCCACCTC ATTGCATCAC TCCTAGTCAA
    ACTTTGCACC TTTACACGTC CTCTAATCCG AGAGGGGAAC ATAATAGAGA TGAGGACAGT
    TTAGAGAGGG GCAGCCAAGA AGAACCAGAG GATGGAGCTT TTCAGGAAAG CGAGGAGGAC
    TTGGATGAGC ATGAGGAGGC GGAGGTGAAA GAAGACGAGG TGGAGGAGGA AGAACTTGAG
    GAAGAGATTG AGGAGGAAGA GGTTGCACTG GAACTTATGG CCCCGCAGGC CAAACGGCGG
    TGCAGAGAGG TCATCCCGAA CTGGGACTCC CTGCAGGATG GGGAAGACTC CCTAGATGAG
    ATGCTGCAAT ATCTGGGATA CTCCAGCCCA GAGTGTCTGC AACGTACTGG TGGACCTCTC
    AACATACCTG CCCCACCTCC AAACTGTGTG TCCGAGAAAC AGGAGAATGA TGTCATCAAT
    GCCATCCTGA AGCAGTGTGC CGAGGAGCAG GACTTCACAA TTCACCGTGG TAATGAGCTG
    AACATGAGGG CCGTCGTTCA GCAGGAGCCA AGCCCACAGA GACCACAGTC TGCCTTCTAC
    TACTGCAAAC TGCTCCTCAA CATACTAGGC ATGAACTCTT GGGAGAAGAG GAACAGTTTC
    CACCTGCTGA AAAAGAATGA AAAACTACTG AGAGAGCTGA AGAATCTGGA CTCCAGGCAG
    TGTCGTGAAA CACACAAAAT CGCGGTGTTT TACGTGGCAG AGGGACAAGA AGACAAGCAC
    TCCATTCTGT CCAACACAGC CGGGAGTCAG GCGTATGAAG ACTTTGTATC AGGCCTGGGC
    TGGGAGGTGA ACCTGACGAG TCACTGTGGC TTTATGGGTG GCCTGCAGCG GAACAAGAGC
    ACGGGTTTTA CCATGCCTTA CTTTGCCACC TCTACAGTGG AAGTGATGTT CCACGTCTCC
    ACACGCATGC CTCCAGACTC AGACGACTCG CTCACTAAAA AGTTGAGACA CCTGGGTAAT
    GATGAAGTGC ACATCGTGTG GTCTGAGCAC TCGCGGGACT ATCGGAGAGG CGTCATTCCT
    ACTGAGTTTG GAGACGTGCT CATCGTCATC TACCCTATGA AGAACCACAT GTACAGCATC
    CAGATCATCA AGAAACCTGA GGTGCCGTTC TTTGGTCCCT TGTTTGACGG AGCCATAGTG
    GATGGGACTA TATTGCCCAC AGTGGTGCGA GCGACAGCCA TCAACGCCAG CAGAGCTCTG
    AAATCCCTCA TCCCTCTATA CCAAAACTTC TATGAAGAGA GGGCCAGATA TCTGGAGACG
    ATAGTCCAAC ATCACTTGGA GCCCACGACA TTTGAGGACT ACGCCGCTCG TGTCTTTTGT
    CCTGCGCCTT TCCACTATCT TCCGTCTGAA GCAGATCATA ATCTATCACT TGTGTATATC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322988.1
    CDS654..8036
    Translation

    Target ORF information:

    RefSeq Version XM_021467313.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGGTG CACTTCCTGT CTCTGACAGT CCCTCTCACT CCCCCCACTT TACAGCCACC
    ACCACCACCA TTGAGTTCCC CCACAGTTCC CCAAACCCAC AGCAGCTCAC TGACCCACAG
    CAGCTCCCCG ACAGCCAGGC GTCCCTGGAG GTGGCCGAAA GTATCTATGA CCATCTCTGC
    CAAATGCCAG GCCAGCAGCC CCCCTTTATC CCCGATTGGT CCTGTCCAGC CGCGGCCTCG
    GCCAATCAAC CCGAGGGCGA AGCGGAGGAG GACGTCTTCA GTGCTTTTCG GGAGTTTTGT
    CACAGGGAGA ATGTGAAAAT GGAAGCAGGT GCAGATTTAA AGCAGGTGCA GTTTGAGGGC
    GATTGGGCTC TAGAATGGGA TAGAGAGATG GAGCAGAGCG AGAAGAGTTT GTATGAGTGC
    TTAGAGGAGT GTGATTGGGA TTTGAGTGTT AGTCCGCAGC CAGCAGAGGA CGGTAAAGGA
    GCACAGCTTC AGCGGCAGGA CGCCACCGAC AGGGCTAGCG AGAGTGAGGT TAGTCCACAG
    GCTTCGCATG GACCCATGTT GTCAAATATT GATCACAAGA AACGCCACAG CAGTGCGGTC
    CATGTCAGTT TCCGGCCCTC CACAGAGTCT GTGCTGTTCC ACAACCCTTT GGACCCCAAG
    GAGGCACACT GGAAAACCCG GCTTCGCCGT CTCGGGCACT TCAGCAGCCA CTCGGTTGGG
    GCGAAAGGTG TAGGGGAGGG GGTCACCTGT TGTGAAGTGG TGGAGCGGGA AAGAGGCTCC
    ATCAGCGGGG CTAGACGTGG ACGACTTGGT CGTCCGACTC TTCGGCCTCG AGGGTCCCGA
    TCTAGATCTC AGGAGTCAGG GTGCAGCCCC TCACGACAGC AGCAGGTGGC TCTGTTGGGT
    GGGGTGTATA AGTCTATGGT CCACGCTCTC TCCAAACCCA AGGGCCAGGC AGCATCGCCC
    CAGCGCCACA GCAAATCGCC CGCCACTGAG GCCCACCTGA AGGACCTGTA TGCTAGCGTT
    ATGGGCTATT TTGGAAGAAA AACATCAGCC AATAAAGACG AGCTGACGCC GAAGATGCGG
    CCCATGTCTA ACGATGCTGG CAGCAGCAAC CCCAATGTCA GTGACCTCAT GGACGAGTTC
    ATCCAGGAGA GACTGAGAGC CAGAGGCAAT TCTGGCATGA CGAGGCGCGG CAGTAGCCCA
    GGAAGTCTGG AGATCCCTAA AGATCTGCCA GAGCTGCTGA ACCGTCAGAA CGCCACACGT
    CCCGCAGACG ACCCCGGTGT GCCCTCTGAG TGGACCTCGC CTGCCAGTGC CAGTGGAAGT
    GACCTCATAA GCTCTGACAG CCAATCGGAT TCCTTTAACG CTTTTCAATA TGACAGCAAA
    TTCGAAAATT TCAGCTTCCC CCCTGAGACG TGTGCTTTGG CGTCTGCAGA TCAGGATAGT
    TTGAGTGGGA CAGGACAGAC AGCGGAGGAA CAGGAGCTTT CCAGTCTCAC AACACTTCAC
    ATCGACTCTG AGACCAGCAG CCTTAGTCAG CACGGCCTGT CTGCTGATAC TGTCACTATT
    ACTGGCTCTG AGAGCGCATC TCCAATGCAC TCTCTAGGAG GATCTCGATC GCAGACGCCA
    TCTCCTGCAA CCCTGACCGC TGAACACGTC CAGCATAAAG AGCTGCAGCT GGATGAGAAG
    ATGCACCATT CTGTTCTGCA GACCCCTGAT GACCTTGAAA CCAGCGAGTT TCCCACTGAG
    GACTGTAGTG TAATGGCTGG TGGCACTCTG ACTGGCTGGC ATGCTGATGT CGCAACAGTA
    ATGTGGAGGA GAATGCTTGG GATTCTGGGA GATGTCAACT ATATAAAGGA CCCTGAGATC
    CATGCGCAGG TTTTTGACTA CCTCTGCGAA CTCTGGCAGA ACCTGGCCAA GATCAGAGAC
    AATCTCGGGA TCTCCCTTGA CAACCAGTCG TCTCCGCCAC CACCAGATCT GATTCCACCT
    CTGCGGATTC TGACTCCATG GCTTTTCAAA GCGACTATGT TGGGCGAACG CTACAAGCAG
    GGAAAACTGC ACGCCTACAA GCTCATCTGT AAGATCATGA AGAGAAGACA GGACGTTTCC
    CCAAACTCTG ACTTCCTCAC GCACTTCTAC AACATCATGC ACTGCGGCCT GCTCCACCAA
    GACCAGGATA TTGTGAACAC CATCATTAAG CACTGCTCTC CACGCTTCTT CTCTATTGGA
    CTTCCAGGAG CCACAATGCT CATCCTGGAC TTCATCGTGG CAGCTAGTCG TGTCACTGCC
    TGCTCGTCCC TCAATGCTCC ACGGGTGGAG GCTCAGATCT TGTTGGGTTC CCTGGTTTGC
    CTCCCAAACT TGTACAATGA ACTTCCTGCA CTGCATCCTA CAACAGCCGA CATCATCATG
    ACCAAATTCA CTGATGTCAA GGAGCATATA ATCAAGCACA TCCTGACCTC TGCCAGAGAT
    GAGCCTTCTG CTCCAGCCAG GTGTGTGGCG CTCTGTAGTC TGGGTATTTG GCTGTGTGAA
    GAGCTGGTTC ATGGCACTCA GCATCCGCAG ATCAAAGAAG CGCTTAACGT CATCTGTGTC
    ACTCTCAAGT TCTCCAATAA AAACGTCGCC CTGGTGGCTT CAGACATTTT GCACCTGCTG
    ATAAACTACG TGGATGAACT GGAGAAATTT CCACCCAACA CTCCAAAGAA AATAGTGGAG
    GTTCTTATTG CAACCATCAC TTACCTTCTT CCAGCCACAG AGTCCTCTCC TCATGAGCTG
    GACAAGAGGC TGGTTGTTTC TCTACTGCTG TGCTTGCTGG ACTGGGTGAT GGCTCTACCT
    CCGAAAACAC TCCTCCAGCC GGTCCAAGGA GCCTCGGATA AGGAGAAAAC TGACAAGTCT
    GTCCTCAGCT GCATCTATAA AGTCCTCCAT GGTTGTGTCT ATGGGGCTCA GAGCTTCAGT
    AACCCCAGTT ACTTCCCCAT TCACCTGTCA GACTTAAGCA GCCCAGACTA CGACCCCTTC
    TTACTACTAG AAAACCTTAA GGAGCCGGAG CCACTCCATT CACCTGACTC TGAACGCTCC
    TCCAAACTGC AACCAGTAAC TGAAGTGAGG AGCCGCATTC AGCAAGGCCT TGTTTCAGTT
    GCGGCACGCA CTGTCATATC TCACCTAGTC AACCATCTGG GTCACTATCC CATGAGTGGT
    GGTCCGGCCA CTCTGACGAG CCAGGTGTGC GAGAACCAGG ACAACCCATA CAGTGAGAGC
    GCAGACCTGA CCCCTGAACT CTTTGACGCC CCCAATTTGC AGTTCTTCGT GCTTAATGGC
    ACCACCCTGC TGTCCTACTT ACAAATTCGG GCTGAAGATG GCCTGCCCGG TGGTGGGATG
    TCAGCTGGCC TCACCACCAC CAATGCTTGT GTTCGGGTGA TTGTGAGGGA CATTTCTGGT
    AAACACTCCT GGGACTCAGC TGTTCTGTAT GGTCCACCTC ATTGCATCAC TCCTAGTCAA
    ACTTTGCACC TTTACACGTC CTCTAATCCG AGAGGGGAAC ATAATAGAGA TGAGGACAGT
    TTAGAGAGGG GCAGCCAAGA AGAACCAGAG GATGGAGCTT TTCAGGAAAG CGAGGAGGAC
    TTGGATGAGC ATGAGGAGGC GGAGGTGAAA GAAGACGAGG TGGAGGAGGA AGAACTTGAG
    GAAGAGATTG AGGAGGAAGA GGTTGCACTG GAACTTATGG CCCCGCAGGC CAAACGGCGG
    TGCAGAGAGG TCATCCCGAA CTGGGACTCC CTGCAGGATG GGGAAGACTC CCTAGATGAG
    ATGCTGCAAT ATCTGGGATA CTCCAGCCCA GAGTGTCTGC AACGTACTGG TGGACCTCTC
    AACATACCTG CCCCACCTCC AAACTGTGTG TCCGAGAAAC AGGAGAATGA TGTCATCAAT
    GCCATCCTGA AGCAGTGTGC CGAGGAGCAG GACTTCACAA TTCACCGTGG TAATGAGCTG
    AACATGAGGG CCGTCGTTCA GCAGGAGCCA AGCCCACAGA GACCACAGTC TGCCTTCTAC
    TACTGCAAAC TGCTCCTCAA CATACTAGGC ATGAACTCTT GGGAGAAGAG GAACAGTTTC
    CACCTGCTGA AAAAGAATGA AAAACTACTG AGAGAGCTGA AGAATCTGGA CTCCAGGCAG
    TGTCGTGAAA CACACAAAAT CGCGGTGTTT TACGTGGCAG AGGGACAAGA AGACAAGCAC
    TCCATTCTGT CCAACACAGC CGGGAGTCAG GCGTATGAAG ACTTTGTATC AGGCCTGGGC
    TGGGAGGTGA ACCTGACGAG TCACTGTGGC TTTATGGGTG GCCTGCAGCG GAACAAGAGC
    ACGGGTTTTA CCATGCCTTA CTTTGCCACC TCTACAGTGG AAGTGATGTT CCACGTCTCC
    ACACGCATGC CTCCAGACTC AGACGACTCG CTCACTAAAA AGTTGAGACA CCTGGGTAAT
    GATGAAGTGC ACATCGTGTG GTCTGAGCAC TCGCGGGACT ATCGGAGAGG CGTCATTCCT
    ACTGAGTTTG GAGACGTGCT CATCGTCATC TACCCTATGA AGAACCACAT GTACAGCATC
    CAGATCATCA AGAAACCTGA GGTGCCGTTC TTTGGTCCCT TGTTTGACGG AGCCATAGTG
    GATGGGACTA TATTGCCCAC AGTGGTGCGA GCGACAGCCA TCAACGCCAG CAGAGCTCTG
    AAATCCCTCA TCCCTCTATA CCAAAACTTC TATGAAGAGA GGGCCAGATA TCTGGAGACG
    ATAGTCCAAC ATCACTTGGA GCCCACGACA TTTGAGGACT ACGCCGCTCG TGTCTTTTGT
    CCTGCGCCTT TCCACTATCT TCCGTCTGAA GCAGATCATA ATCTATCACT TGTGTATATC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149652
    Clone ID Related Accession (Same CDS sequence) XM_021467314.1
    Accession Version XM_021467314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGCCA ATAAAGACGA GCTGACGCCG AAGATGCGGC CCATGTCTAA CGATGCTGGC
    AGCAGCAACC CCAATGTCAG TGACCTCATG GACGAGTTCA TCCAGGAGAG ACTGAGAGCC
    AGAGGCAATT CTGGCATGAC GAGGCGCGGC AGTAGCCCAG GAAGTCTGGA GATCCCTAAA
    GATCTGCCAG AGCTGCTGAA CCGTCAGAAC GCCACACGTC CCGCAGACGA CCCCGGTGTG
    CCCTCTGAGT GGACCTCGCC TGCCAGTGCC AGTGGAAGTG ACCTCATAAG CTCTGACAGC
    CAATCGGATT CCTTTAACGC TTTTCAATAT GACAGCAAAT TCGAAAATTT CAGCTTCCCC
    CCTGAGACGT GTGCTTTGGC GTCTGCAGAT CAGGATAGTT TGAGTGGGAC AGGACAGACA
    GCGGAGGAAC AGGAGCTTTC CAGTCTCACA ACACTTCACA TCGACTCTGA GACCAGCAGC
    CTTAGTCAGC ACGGCCTGTC TGCTGATACT GTCACTATTA CTGGCTCTGA GAGCGCATCT
    CCAATGCACT CTCTAGGAGG ATCTCGATCG CAGACGCCAT CTCCTGCAAC CCTGACCGCT
    GAACACGTCC AGCATAAAGA GCTGCAGCTG GATGAGAAGA TGCACCATTC TGTTCTGCAG
    ACCCCTGATG ACCTTGAAAC CAGCGAGTTT CCCACTGAGG ACTGTAGTGT AATGGCTGGT
    GGCACTCTGA CTGGCTGGCA TGCTGATGTC GCAACAGTAA TGTGGAGGAG AATGCTTGGG
    ATTCTGGGAG ATGTCAACTA TATAAAGGAC CCTGAGATCC ATGCGCAGGT TTTTGACTAC
    CTCTGCGAAC TCTGGCAGAA CCTGGCCAAG ATCAGAGACA ATCTCGGGAT CTCCCTTGAC
    AACCAGTCGT CTCCGCCACC ACCAGATCTG ATTCCACCTC TGCGGATTCT GACTCCATGG
    CTTTTCAAAG CGACTATGTT GGGCGAACGC TACAAGCAGG GAAAACTGCA CGCCTACAAG
    CTCATCTGTA AGATCATGAA GAGAAGACAG GACGTTTCCC CAAACTCTGA CTTCCTCACG
    CACTTCTACA ACATCATGCA CTGCGGCCTG CTCCACCAAG ACCAGGATAT TGTGAACACC
    ATCATTAAGC ACTGCTCTCC ACGCTTCTTC TCTATTGGAC TTCCAGGAGC CACAATGCTC
    ATCCTGGACT TCATCGTGGC AGCTAGTCGT GTCACTGCCT GCTCGTCCCT CAATGCTCCA
    CGGGTGGAGG CTCAGATCTT GTTGGGTTCC CTGGTTTGCC TCCCAAACTT GTACAATGAA
    CTTCCTGCAC TGCATCCTAC AACAGCCGAC ATCATCATGA CCAAATTCAC TGATGTCAAG
    GAGCATATAA TCAAGCACAT CCTGACCTCT GCCAGAGATG AGCCTTCTGC TCCAGCCAGG
    TGTGTGGCGC TCTGTAGTCT GGGTATTTGG CTGTGTGAAG AGCTGGTTCA TGGCACTCAG
    CATCCGCAGA TCAAAGAAGC GCTTAACGTC ATCTGTGTCA CTCTCAAGTT CTCCAATAAA
    AACGTCGCCC TGGTGGCTTC AGACATTTTG CACCTGCTGA TAAACTACGT GGATGAACTG
    GAGAAATTTC CACCCAACAC TCCAAAGAAA ATAGTGGAGG TTCTTATTGC AACCATCACT
    TACCTTCTTC CAGCCACAGA GTCCTCTCCT CATGAGCTGG ACAAGAGGCT GGTTGTTTCT
    CTACTGCTGT GCTTGCTGGA CTGGGTGATG GCTCTACCTC CGAAAACACT CCTCCAGCCG
    GTCCAAGGAG CCTCGGATAA GGAGAAAACT GACAAGTCTG TCCTCAGCTG CATCTATAAA
    GTCCTCCATG GTTGTGTCTA TGGGGCTCAG AGCTTCAGTA ACCCCAGTTA CTTCCCCATT
    CACCTGTCAG ACTTAAGCAG CCCAGACTAC GACCCCTTCT TACTACTAGA AAACCTTAAG
    GAGCCGGAGC CACTCCATTC ACCTGACTCT GAACGCTCCT CCAAACTGCA ACCAGTAACT
    GAAGTGAGGA GCCGCATTCA GCAAGGCCTT GTTTCAGTTG CGGCACGCAC TGTCATATCT
    CACCTAGTCA ACCATCTGGG TCACTATCCC ATGAGTGGTG GTCCGGCCAC TCTGACGAGC
    CAGGTGTGCG AGAACCAGGA CAACCCATAC AGTGAGAGCG CAGACCTGAC CCCTGAACTC
    TTTGACGCCC CCAATTTGCA GTTCTTCGTG CTTAATGGCA CCACCCTGCT GTCCTACTTA
    CAAATTCGGG CTGAAGATGG CCTGCCCGGT GGTGGGATGT CAGCTGGCCT CACCACCACC
    AATGCTTGTG TTCGGGTGAT TGTGAGGGAC ATTTCTGGTA AACACTCCTG GGACTCAGCT
    GTTCTGTATG GTCCACCTCA TTGCATCACT CCTAGTCAAA CTTTGCACCT TTACACGTCC
    TCTAATCCGA GAGGGGAACA TAATAGAGAT GAGGACAGTT TAGAGAGGGG CAGCCAAGAA
    GAACCAGAGG ATGGAGCTTT TCAGGAAAGC GAGGAGGACT TGGATGAGCA TGAGGAGGCG
    GAGGTGAAAG AAGACGAGGT GGAGGAGGAA GAACTTGAGG AAGAGATTGA GGAGGAAGAG
    GTTGCACTGG AACTTATGGC CCCGCAGGCC AAACGGCGGT GCAGAGAGGT CATCCCGAAC
    TGGGACTCCC TGCAGGATGG GGAAGACTCC CTAGATGAGA TGCTGCAATA TCTGGGATAC
    TCCAGCCCAG AGTGTCTGCA ACGTACTGGT GGACCTCTCA ACATACCTGC CCCACCTCCA
    AACTGTGTGT CCGAGAAACA GGAGAATGAT GTCATCAATG CCATCCTGAA GCAGTGTGCC
    GAGGAGCAGG ACTTCACAAT TCACCGTGGT AATGAGCTGA ACATGAGGGC CGTCGTTCAG
    CAGGAGCCAA GCCCACAGAG ACCACAGTCT GCCTTCTACT ACTGCAAACT GCTCCTCAAC
    ATACTAGGCA TGAACTCTTG GGAGAAGAGG AACAGTTTCC ACCTGCTGAA AAAGAATGAA
    AAACTACTGA GAGAGCTGAA GAATCTGGAC TCCAGGCAGT GTCGTGAAAC ACACAAAATC
    GCGGTGTTTT ACGTGGCAGA GGGACAAGAA GACAAGCACT CCATTCTGTC CAACACAGCC
    GGGAGTCAGG CGTATGAAGA CTTTGTATCA GGCCTGGGCT GGGAGGTGAA CCTGACGAGT
    CACTGTGGCT TTATGGGTGG CCTGCAGCGG AACAAGAGCA CGGGTTTTAC CATGCCTTAC
    TTTGCCACCT CTACAGTGGA AGTGATGTTC CACGTCTCCA CACGCATGCC TCCAGACTCA
    GACGACTCGC TCACTAAAAA GTTGAGACAC CTGGGTAATG ATGAAGTGCA CATCGTGTGG
    TCTGAGCACT CGCGGGACTA TCGGAGAGGC GTCATTCCTA CTGAGTTTGG AGACGTGCTC
    ATCGTCATCT ACCCTATGAA GAACCACATG TACAGCATCC AGATCATCAA GAAACCTGAG
    GTGCCGTTCT TTGGTCCCTT GTTTGACGGA GCCATAGTGG ATGGGACTAT ATTGCCCACA
    GTGGTGCGAG CGACAGCCAT CAACGCCAGC AGAGCTCTGA AATCCCTCAT CCCTCTATAC
    CAAAACTTCT ATGAAGAGAG GGCCAGATAT CTGGAGACGA TAGTCCAACA TCACTTGGAG
    CCCACGACAT TTGAGGACTA CGCCGCTCGT GTCTTTTGTC CTGCGCCTTT CCACTATCTT
    CCGTCTGAAG CAGAGAGAAA TGCTGTGATT TTTCTGCCCC TCCCTAAACC TCCACTAGAA
    AACATTGGGC TCACCAAGGT AAAGGGCAAA GGCTTGAAAC GGAAGGTCTT TCACGCTAGC
    TGTGAATGGC TCATGCCTCG AGAGTCAGCT GGCAGAAAGT CCAGCAATGC AGGTCGACGG
    GAGCGACTTA GCCTCGCCAA TGTCACCTCG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322989.1
    CDS599..7114
    Translation

    Target ORF information:

    RefSeq Version XM_021467314.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGCCA ATAAAGACGA GCTGACGCCG AAGATGCGGC CCATGTCTAA CGATGCTGGC
    AGCAGCAACC CCAATGTCAG TGACCTCATG GACGAGTTCA TCCAGGAGAG ACTGAGAGCC
    AGAGGCAATT CTGGCATGAC GAGGCGCGGC AGTAGCCCAG GAAGTCTGGA GATCCCTAAA
    GATCTGCCAG AGCTGCTGAA CCGTCAGAAC GCCACACGTC CCGCAGACGA CCCCGGTGTG
    CCCTCTGAGT GGACCTCGCC TGCCAGTGCC AGTGGAAGTG ACCTCATAAG CTCTGACAGC
    CAATCGGATT CCTTTAACGC TTTTCAATAT GACAGCAAAT TCGAAAATTT CAGCTTCCCC
    CCTGAGACGT GTGCTTTGGC GTCTGCAGAT CAGGATAGTT TGAGTGGGAC AGGACAGACA
    GCGGAGGAAC AGGAGCTTTC CAGTCTCACA ACACTTCACA TCGACTCTGA GACCAGCAGC
    CTTAGTCAGC ACGGCCTGTC TGCTGATACT GTCACTATTA CTGGCTCTGA GAGCGCATCT
    CCAATGCACT CTCTAGGAGG ATCTCGATCG CAGACGCCAT CTCCTGCAAC CCTGACCGCT
    GAACACGTCC AGCATAAAGA GCTGCAGCTG GATGAGAAGA TGCACCATTC TGTTCTGCAG
    ACCCCTGATG ACCTTGAAAC CAGCGAGTTT CCCACTGAGG ACTGTAGTGT AATGGCTGGT
    GGCACTCTGA CTGGCTGGCA TGCTGATGTC GCAACAGTAA TGTGGAGGAG AATGCTTGGG
    ATTCTGGGAG ATGTCAACTA TATAAAGGAC CCTGAGATCC ATGCGCAGGT TTTTGACTAC
    CTCTGCGAAC TCTGGCAGAA CCTGGCCAAG ATCAGAGACA ATCTCGGGAT CTCCCTTGAC
    AACCAGTCGT CTCCGCCACC ACCAGATCTG ATTCCACCTC TGCGGATTCT GACTCCATGG
    CTTTTCAAAG CGACTATGTT GGGCGAACGC TACAAGCAGG GAAAACTGCA CGCCTACAAG
    CTCATCTGTA AGATCATGAA GAGAAGACAG GACGTTTCCC CAAACTCTGA CTTCCTCACG
    CACTTCTACA ACATCATGCA CTGCGGCCTG CTCCACCAAG ACCAGGATAT TGTGAACACC
    ATCATTAAGC ACTGCTCTCC ACGCTTCTTC TCTATTGGAC TTCCAGGAGC CACAATGCTC
    ATCCTGGACT TCATCGTGGC AGCTAGTCGT GTCACTGCCT GCTCGTCCCT CAATGCTCCA
    CGGGTGGAGG CTCAGATCTT GTTGGGTTCC CTGGTTTGCC TCCCAAACTT GTACAATGAA
    CTTCCTGCAC TGCATCCTAC AACAGCCGAC ATCATCATGA CCAAATTCAC TGATGTCAAG
    GAGCATATAA TCAAGCACAT CCTGACCTCT GCCAGAGATG AGCCTTCTGC TCCAGCCAGG
    TGTGTGGCGC TCTGTAGTCT GGGTATTTGG CTGTGTGAAG AGCTGGTTCA TGGCACTCAG
    CATCCGCAGA TCAAAGAAGC GCTTAACGTC ATCTGTGTCA CTCTCAAGTT CTCCAATAAA
    AACGTCGCCC TGGTGGCTTC AGACATTTTG CACCTGCTGA TAAACTACGT GGATGAACTG
    GAGAAATTTC CACCCAACAC TCCAAAGAAA ATAGTGGAGG TTCTTATTGC AACCATCACT
    TACCTTCTTC CAGCCACAGA GTCCTCTCCT CATGAGCTGG ACAAGAGGCT GGTTGTTTCT
    CTACTGCTGT GCTTGCTGGA CTGGGTGATG GCTCTACCTC CGAAAACACT CCTCCAGCCG
    GTCCAAGGAG CCTCGGATAA GGAGAAAACT GACAAGTCTG TCCTCAGCTG CATCTATAAA
    GTCCTCCATG GTTGTGTCTA TGGGGCTCAG AGCTTCAGTA ACCCCAGTTA CTTCCCCATT
    CACCTGTCAG ACTTAAGCAG CCCAGACTAC GACCCCTTCT TACTACTAGA AAACCTTAAG
    GAGCCGGAGC CACTCCATTC ACCTGACTCT GAACGCTCCT CCAAACTGCA ACCAGTAACT
    GAAGTGAGGA GCCGCATTCA GCAAGGCCTT GTTTCAGTTG CGGCACGCAC TGTCATATCT
    CACCTAGTCA ACCATCTGGG TCACTATCCC ATGAGTGGTG GTCCGGCCAC TCTGACGAGC
    CAGGTGTGCG AGAACCAGGA CAACCCATAC AGTGAGAGCG CAGACCTGAC CCCTGAACTC
    TTTGACGCCC CCAATTTGCA GTTCTTCGTG CTTAATGGCA CCACCCTGCT GTCCTACTTA
    CAAATTCGGG CTGAAGATGG CCTGCCCGGT GGTGGGATGT CAGCTGGCCT CACCACCACC
    AATGCTTGTG TTCGGGTGAT TGTGAGGGAC ATTTCTGGTA AACACTCCTG GGACTCAGCT
    GTTCTGTATG GTCCACCTCA TTGCATCACT CCTAGTCAAA CTTTGCACCT TTACACGTCC
    TCTAATCCGA GAGGGGAACA TAATAGAGAT GAGGACAGTT TAGAGAGGGG CAGCCAAGAA
    GAACCAGAGG ATGGAGCTTT TCAGGAAAGC GAGGAGGACT TGGATGAGCA TGAGGAGGCG
    GAGGTGAAAG AAGACGAGGT GGAGGAGGAA GAACTTGAGG AAGAGATTGA GGAGGAAGAG
    GTTGCACTGG AACTTATGGC CCCGCAGGCC AAACGGCGGT GCAGAGAGGT CATCCCGAAC
    TGGGACTCCC TGCAGGATGG GGAAGACTCC CTAGATGAGA TGCTGCAATA TCTGGGATAC
    TCCAGCCCAG AGTGTCTGCA ACGTACTGGT GGACCTCTCA ACATACCTGC CCCACCTCCA
    AACTGTGTGT CCGAGAAACA GGAGAATGAT GTCATCAATG CCATCCTGAA GCAGTGTGCC
    GAGGAGCAGG ACTTCACAAT TCACCGTGGT AATGAGCTGA ACATGAGGGC CGTCGTTCAG
    CAGGAGCCAA GCCCACAGAG ACCACAGTCT GCCTTCTACT ACTGCAAACT GCTCCTCAAC
    ATACTAGGCA TGAACTCTTG GGAGAAGAGG AACAGTTTCC ACCTGCTGAA AAAGAATGAA
    AAACTACTGA GAGAGCTGAA GAATCTGGAC TCCAGGCAGT GTCGTGAAAC ACACAAAATC
    GCGGTGTTTT ACGTGGCAGA GGGACAAGAA GACAAGCACT CCATTCTGTC CAACACAGCC
    GGGAGTCAGG CGTATGAAGA CTTTGTATCA GGCCTGGGCT GGGAGGTGAA CCTGACGAGT
    CACTGTGGCT TTATGGGTGG CCTGCAGCGG AACAAGAGCA CGGGTTTTAC CATGCCTTAC
    TTTGCCACCT CTACAGTGGA AGTGATGTTC CACGTCTCCA CACGCATGCC TCCAGACTCA
    GACGACTCGC TCACTAAAAA GTTGAGACAC CTGGGTAATG ATGAAGTGCA CATCGTGTGG
    TCTGAGCACT CGCGGGACTA TCGGAGAGGC GTCATTCCTA CTGAGTTTGG AGACGTGCTC
    ATCGTCATCT ACCCTATGAA GAACCACATG TACAGCATCC AGATCATCAA GAAACCTGAG
    GTGCCGTTCT TTGGTCCCTT GTTTGACGGA GCCATAGTGG ATGGGACTAT ATTGCCCACA
    GTGGTGCGAG CGACAGCCAT CAACGCCAGC AGAGCTCTGA AATCCCTCAT CCCTCTATAC
    CAAAACTTCT ATGAAGAGAG GGCCAGATAT CTGGAGACGA TAGTCCAACA TCACTTGGAG
    CCCACGACAT TTGAGGACTA CGCCGCTCGT GTCTTTTGTC CTGCGCCTTT CCACTATCTT
    CCGTCTGAAG CAGAGAGAAA TGCTGTGATT TTTCTGCCCC TCCCTAAACC TCCACTAGAA
    AACATTGGGC TCACCAAGGT AAAGGGCAAA GGCTTGAAAC GGAAGGTCTT TCACGCTAGC
    TGTGAATGGC TCATGCCTCG AGAGTCAGCT GGCAGAAAGT CCAGCAATGC AGGTCGACGG
    GAGCGACTTA GCCTCGCCAA TGTCACCTCG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149653
    Clone ID Related Accession (Same CDS sequence) XM_021467315.1
    Accession Version XM_021467315.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGA CCTGGAGGAG
    CCTCCTATGA TCCAGCGTGG GCCACGGAAC CGCCACTGCT CTCAGAGCGA GGAGACGCCG
    ACGTCTGAAG TTTTTTCGGC CTCCGGTGAA CTGGAGCAGC CTCCGCTTCC CCGCAGTAGC
    AGTGCCTCCG ACATCATGGA GCCCTTCATC GCAGAACGGG CCAAAGCCAA TAAAGACGAG
    CTGACGCCGA AGATGCGGCC CATGTCTAAC GATGCTGGCA GCAGCAACCC CAATGTCAGT
    GACCTCATGG ACGAGTTCAT CCAGGAGAGA CTGAGAGCCA GAGGCAATTC TGGCATGACG
    AGGCGCGGCA GTAGCCCAGG AAGTCTGGAG ATCCCTAAAG ATCTGCCAGA GCTGCTGAAC
    CGTCAGAACG CCACACGTCC CGCAGACGAC CCCGGTGTGC CCTCTGAGTG GACCTCGCCT
    GCCAGTGCCA GTGGAAGTGA CCTCATAAGC TCTGACAGCC AATCGGATTC CTTTAACGCT
    TTTCAATATG ACAGCAAATT CGAAAATTTC AGCTTCCCCC CTGAGACGTG TGCTTTGGCG
    TCTGCAGATC AGGATAGTTT GAGTGGGACA GGACAGACAG CGGAGGAACA GGAGCTTTCC
    AGTCTCACAA CACTTCACAT CGACTCTGAG ACCAGCAGCC TTAGTCAGCA CGGCCTGTCT
    GCTGATACTG TCACTATTAC TGGCTCTGAG AGCGCATCTC CAATGCACTC TCTAGGAGGA
    TCTCGATCGC AGACGCCATC TCCTGCAACC CTGACCGCTG AACACGTCCA GCATAAAGAG
    CTGCAGCTGG ATGAGAAGAT GCACCATTCT GTTCTGCAGA CCCCTGATGA CCTTGAAACC
    AGCGAGTTTC CCACTGAGGA CTGTAGTGTA ATGGCTGGTG GCACTCTGAC TGGCTGGCAT
    GCTGATGTCG CAACAGTAAT GTGGAGGAGA ATGCTTGGGA TTCTGGGAGA TGTCAACTAT
    ATAAAGGACC CTGAGATCCA TGCGCAGGTT TTTGACTACC TCTGCGAACT CTGGCAGAAC
    CTGGCCAAGA TCAGAGACAA TCTCGGGATC TCCCTTGACA ACCAGTCGTC TCCGCCACCA
    CCAGATCTGA TTCCACCTCT GCGGATTCTG ACTCCATGGC TTTTCAAAGC GACTATGTTG
    GGCGAACGCT ACAAGCAGGG AAAACTGCAC GCCTACAAGC TCATCTGTAA GATCATGAAG
    AGAAGACAGG ACGTTTCCCC AAACTCTGAC TTCCTCACGC ACTTCTACAA CATCATGCAC
    TGCGGCCTGC TCCACCAAGA CCAGGATATT GTGAACACCA TCATTAAGCA CTGCTCTCCA
    CGCTTCTTCT CTATTGGACT TCCAGGAGCC ACAATGCTCA TCCTGGACTT CATCGTGGCA
    GCTAGTCGTG TCACTGCCTG CTCGTCCCTC AATGCTCCAC GGGTGGAGGC TCAGATCTTG
    TTGGGTTCCC TGGTTTGCCT CCCAAACTTG TACAATGAAC TTCCTGCACT GCATCCTACA
    ACAGCCGACA TCATCATGAC CAAATTCACT GATGTCAAGG AGCATATAAT CAAGCACATC
    CTGACCTCTG CCAGAGATGA GCCTTCTGCT CCAGCCAGGT GTGTGGCGCT CTGTAGTCTG
    GGTATTTGGC TGTGTGAAGA GCTGGTTCAT GGCACTCAGC ATCCGCAGAT CAAAGAAGCG
    CTTAACGTCA TCTGTGTCAC TCTCAAGTTC TCCAATAAAA ACGTCGCCCT GGTGGCTTCA
    GACATTTTGC ACCTGCTGAT AAACTACGTG GATGAACTGG AGAAATTTCC ACCCAACACT
    CCAAAGAAAA TAGTGGAGGT TCTTATTGCA ACCATCACTT ACCTTCTTCC AGCCACAGAG
    TCCTCTCCTC ATGAGCTGGA CAAGAGGCTG GTTGTTTCTC TACTGCTGTG CTTGCTGGAC
    TGGGTGATGG CTCTACCTCC GAAAACACTC CTCCAGCCGG TCCAAGGAGC CTCGGATAAG
    GAGAAAACTG ACAAGTCTGT CCTCAGCTGC ATCTATAAAG TCCTCCATGG TTGTGTCTAT
    GGGGCTCAGA GCTTCAGTAA CCCCAGTTAC TTCCCCATTC ACCTGTCAGA CTTAAGCAGC
    CCAGACTACG ACCCCTTCTT ACTACTAGAA AACCTTAAGG AGCCGGAGCC ACTCCATTCA
    CCTGACTCTG AACGCTCCTC CAAACTGCAA CCAGTAACTG AAGTGAGGAG CCGCATTCAG
    CAAGGCCTTG TTTCAGTTGC GGCACGCACT GTCATATCTC ACCTAGTCAA CCATCTGGGT
    CACTATCCCA TGAGTGGTGG TCCGGCCACT CTGACGAGCC AGGTGTGCGA GAACCAGGAC
    AACCCATACA GTGAGAGCGC AGACCTGACC CCTGAACTCT TTGACGCCCC CAATTTGCAG
    TTCTTCGTGC TTAATGGCAC CACCCTGCTG TCCTACTTAC AAATTCGGGC TGAAGATGGC
    CTGCCCGGTG GTGGGATGTC AGCTGGCCTC ACCACCACCA ATGCTTGTGT TCGGGTGATT
    GTGAGGGACA TTTCTGGTAA ACACTCCTGG GACTCAGCTG TTCTGTATGG TCCACCTCAT
    TGCATCACTC CTAGTCAAAC TTTGCACCTT TACACGTCCT CTAATCCGAG AGGGGAACAT
    AATAGAGATG AGGACAGTTT AGAGAGGGGC AGCCAAGAAG AACCAGAGGA TGGAGCTTTT
    CAGGAAAGCG AGGAGGACTT GGATGAGCAT GAGGAGGCGG AGGTGAAAGA AGACGAGGTG
    GAGGAGGAAG AACTTGAGGA AGAGATTGAG GAGGAAGAGG TTGCACTGGA ACTTATGGCC
    CCGCAGGCCA AACGGCGGTG CAGAGAGGTC ATCCCGAACT GGGACTCCCT GCAGGATGGG
    GAAGACTCCC TAGATGAGAT GCTGCAATAT CTGGGATACT CCAGCCCAGA GTGTCTGCAA
    CGTACTGGTG GACCTCTCAA CATACCTGCC CCACCTCCAA ACTGTGTGTC CGAGAAACAG
    GAGAATGATG TCATCAATGC CATCCTGAAG CAGTGTGCCG AGGAGCAGGA CTTCACAATT
    CACCGTGGTA ATGAGCTGAA CATGAGGGCC GTCGTTCAGC AGGAGCCAAG CCCACAGAGA
    CCACAGTCTG CCTTCTACTA CTGCAAACTG CTCCTCAACA TACTAGGCAT GAACTCTTGG
    GAGAAGAGGA ACAGTTTCCA CCTGCTGAAA AAGAATGAAA AACTACTGAG AGAGCTGAAG
    AATCTGGACT CCAGGCAGTG TCGTGAAACA CACAAAATCG CGGTGTTTTA CGTGGCAGAG
    GGACAAGAAG ACAAGCACTC CATTCTGTCC AACACAGCCG GGAGTCAGGC GTATGAAGAC
    TTTGTATCAG GCCTGGGCTG GGAGGTGAAC CTGACGAGTC ACTGTGGCTT TATGGGTGGC
    CTGCAGCGGA ACAAGAGCAC GGGTTTTACC ATGCCTTACT TTGCCACCTC TACAGTGGAA
    GTGATGTTCC ACGTCTCCAC ACGCATGCCT CCAGACTCAG ACGACTCGCT CACTAAAAAG
    TTGAGACACC TGGGTAATGA TGAAGTGCAC ATCGTGTGGT CTGAGCACTC GCGGGACTAT
    CGGAGAGGCG TCATTCCTAC TGAGTTTGGA GACGTGCTCA TCGTCATCTA CCCTATGAAG
    AACCACATGT ACAGCATCCA GATCATCAAG AAACCTGAGG TGCCGTTCTT TGGTCCCTTG
    TTTGACGGAG CCATAGTGGA TGGGACTATA TTGCCCACAG TGGTGCGAGC GACAGCCATC
    AACGCCAGCA GAGCTCTGAA ATCCCTCATC CCTCTATACC AAAACTTCTA TGAAGAGAGG
    GCCAGATATC TGGAGACGAT AGTCCAACAT CACTTGGAGC CCACGACATT TGAGGACTAC
    GCCGCTCGTG TCTTTTGTCC TGCGCCTTTC CACTATCTTC CGTCTGAAGC AGAGAGAAAT
    GCTGTGATTT TTCTGCCCCT CCCTAAACCT CCACTAGAAA ACATTGGGCT CACCAAGGTA
    AAGGGCAAAG GCTTGAAACG GAAGGTCTTT CACGCTAGCT GTGAATGGCT CATGCCTCGA
    GAGTCAGCTG GCAGAAAGTC CAGCAATGCA GGTCGACGGG AGCGACTTAG CCTCGCCAAT
    GTCACCTCGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322990.1
    CDS598..6972
    Translation

    Target ORF information:

    RefSeq Version XM_021467315.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGA CCTGGAGGAG
    CCTCCTATGA TCCAGCGTGG GCCACGGAAC CGCCACTGCT CTCAGAGCGA GGAGACGCCG
    ACGTCTGAAG TTTTTTCGGC CTCCGGTGAA CTGGAGCAGC CTCCGCTTCC CCGCAGTAGC
    AGTGCCTCCG ACATCATGGA GCCCTTCATC GCAGAACGGG CCAAAGCCAA TAAAGACGAG
    CTGACGCCGA AGATGCGGCC CATGTCTAAC GATGCTGGCA GCAGCAACCC CAATGTCAGT
    GACCTCATGG ACGAGTTCAT CCAGGAGAGA CTGAGAGCCA GAGGCAATTC TGGCATGACG
    AGGCGCGGCA GTAGCCCAGG AAGTCTGGAG ATCCCTAAAG ATCTGCCAGA GCTGCTGAAC
    CGTCAGAACG CCACACGTCC CGCAGACGAC CCCGGTGTGC CCTCTGAGTG GACCTCGCCT
    GCCAGTGCCA GTGGAAGTGA CCTCATAAGC TCTGACAGCC AATCGGATTC CTTTAACGCT
    TTTCAATATG ACAGCAAATT CGAAAATTTC AGCTTCCCCC CTGAGACGTG TGCTTTGGCG
    TCTGCAGATC AGGATAGTTT GAGTGGGACA GGACAGACAG CGGAGGAACA GGAGCTTTCC
    AGTCTCACAA CACTTCACAT CGACTCTGAG ACCAGCAGCC TTAGTCAGCA CGGCCTGTCT
    GCTGATACTG TCACTATTAC TGGCTCTGAG AGCGCATCTC CAATGCACTC TCTAGGAGGA
    TCTCGATCGC AGACGCCATC TCCTGCAACC CTGACCGCTG AACACGTCCA GCATAAAGAG
    CTGCAGCTGG ATGAGAAGAT GCACCATTCT GTTCTGCAGA CCCCTGATGA CCTTGAAACC
    AGCGAGTTTC CCACTGAGGA CTGTAGTGTA ATGGCTGGTG GCACTCTGAC TGGCTGGCAT
    GCTGATGTCG CAACAGTAAT GTGGAGGAGA ATGCTTGGGA TTCTGGGAGA TGTCAACTAT
    ATAAAGGACC CTGAGATCCA TGCGCAGGTT TTTGACTACC TCTGCGAACT CTGGCAGAAC
    CTGGCCAAGA TCAGAGACAA TCTCGGGATC TCCCTTGACA ACCAGTCGTC TCCGCCACCA
    CCAGATCTGA TTCCACCTCT GCGGATTCTG ACTCCATGGC TTTTCAAAGC GACTATGTTG
    GGCGAACGCT ACAAGCAGGG AAAACTGCAC GCCTACAAGC TCATCTGTAA GATCATGAAG
    AGAAGACAGG ACGTTTCCCC AAACTCTGAC TTCCTCACGC ACTTCTACAA CATCATGCAC
    TGCGGCCTGC TCCACCAAGA CCAGGATATT GTGAACACCA TCATTAAGCA CTGCTCTCCA
    CGCTTCTTCT CTATTGGACT TCCAGGAGCC ACAATGCTCA TCCTGGACTT CATCGTGGCA
    GCTAGTCGTG TCACTGCCTG CTCGTCCCTC AATGCTCCAC GGGTGGAGGC TCAGATCTTG
    TTGGGTTCCC TGGTTTGCCT CCCAAACTTG TACAATGAAC TTCCTGCACT GCATCCTACA
    ACAGCCGACA TCATCATGAC CAAATTCACT GATGTCAAGG AGCATATAAT CAAGCACATC
    CTGACCTCTG CCAGAGATGA GCCTTCTGCT CCAGCCAGGT GTGTGGCGCT CTGTAGTCTG
    GGTATTTGGC TGTGTGAAGA GCTGGTTCAT GGCACTCAGC ATCCGCAGAT CAAAGAAGCG
    CTTAACGTCA TCTGTGTCAC TCTCAAGTTC TCCAATAAAA ACGTCGCCCT GGTGGCTTCA
    GACATTTTGC ACCTGCTGAT AAACTACGTG GATGAACTGG AGAAATTTCC ACCCAACACT
    CCAAAGAAAA TAGTGGAGGT TCTTATTGCA ACCATCACTT ACCTTCTTCC AGCCACAGAG
    TCCTCTCCTC ATGAGCTGGA CAAGAGGCTG GTTGTTTCTC TACTGCTGTG CTTGCTGGAC
    TGGGTGATGG CTCTACCTCC GAAAACACTC CTCCAGCCGG TCCAAGGAGC CTCGGATAAG
    GAGAAAACTG ACAAGTCTGT CCTCAGCTGC ATCTATAAAG TCCTCCATGG TTGTGTCTAT
    GGGGCTCAGA GCTTCAGTAA CCCCAGTTAC TTCCCCATTC ACCTGTCAGA CTTAAGCAGC
    CCAGACTACG ACCCCTTCTT ACTACTAGAA AACCTTAAGG AGCCGGAGCC ACTCCATTCA
    CCTGACTCTG AACGCTCCTC CAAACTGCAA CCAGTAACTG AAGTGAGGAG CCGCATTCAG
    CAAGGCCTTG TTTCAGTTGC GGCACGCACT GTCATATCTC ACCTAGTCAA CCATCTGGGT
    CACTATCCCA TGAGTGGTGG TCCGGCCACT CTGACGAGCC AGGTGTGCGA GAACCAGGAC
    AACCCATACA GTGAGAGCGC AGACCTGACC CCTGAACTCT TTGACGCCCC CAATTTGCAG
    TTCTTCGTGC TTAATGGCAC CACCCTGCTG TCCTACTTAC AAATTCGGGC TGAAGATGGC
    CTGCCCGGTG GTGGGATGTC AGCTGGCCTC ACCACCACCA ATGCTTGTGT TCGGGTGATT
    GTGAGGGACA TTTCTGGTAA ACACTCCTGG GACTCAGCTG TTCTGTATGG TCCACCTCAT
    TGCATCACTC CTAGTCAAAC TTTGCACCTT TACACGTCCT CTAATCCGAG AGGGGAACAT
    AATAGAGATG AGGACAGTTT AGAGAGGGGC AGCCAAGAAG AACCAGAGGA TGGAGCTTTT
    CAGGAAAGCG AGGAGGACTT GGATGAGCAT GAGGAGGCGG AGGTGAAAGA AGACGAGGTG
    GAGGAGGAAG AACTTGAGGA AGAGATTGAG GAGGAAGAGG TTGCACTGGA ACTTATGGCC
    CCGCAGGCCA AACGGCGGTG CAGAGAGGTC ATCCCGAACT GGGACTCCCT GCAGGATGGG
    GAAGACTCCC TAGATGAGAT GCTGCAATAT CTGGGATACT CCAGCCCAGA GTGTCTGCAA
    CGTACTGGTG GACCTCTCAA CATACCTGCC CCACCTCCAA ACTGTGTGTC CGAGAAACAG
    GAGAATGATG TCATCAATGC CATCCTGAAG CAGTGTGCCG AGGAGCAGGA CTTCACAATT
    CACCGTGGTA ATGAGCTGAA CATGAGGGCC GTCGTTCAGC AGGAGCCAAG CCCACAGAGA
    CCACAGTCTG CCTTCTACTA CTGCAAACTG CTCCTCAACA TACTAGGCAT GAACTCTTGG
    GAGAAGAGGA ACAGTTTCCA CCTGCTGAAA AAGAATGAAA AACTACTGAG AGAGCTGAAG
    AATCTGGACT CCAGGCAGTG TCGTGAAACA CACAAAATCG CGGTGTTTTA CGTGGCAGAG
    GGACAAGAAG ACAAGCACTC CATTCTGTCC AACACAGCCG GGAGTCAGGC GTATGAAGAC
    TTTGTATCAG GCCTGGGCTG GGAGGTGAAC CTGACGAGTC ACTGTGGCTT TATGGGTGGC
    CTGCAGCGGA ACAAGAGCAC GGGTTTTACC ATGCCTTACT TTGCCACCTC TACAGTGGAA
    GTGATGTTCC ACGTCTCCAC ACGCATGCCT CCAGACTCAG ACGACTCGCT CACTAAAAAG
    TTGAGACACC TGGGTAATGA TGAAGTGCAC ATCGTGTGGT CTGAGCACTC GCGGGACTAT
    CGGAGAGGCG TCATTCCTAC TGAGTTTGGA GACGTGCTCA TCGTCATCTA CCCTATGAAG
    AACCACATGT ACAGCATCCA GATCATCAAG AAACCTGAGG TGCCGTTCTT TGGTCCCTTG
    TTTGACGGAG CCATAGTGGA TGGGACTATA TTGCCCACAG TGGTGCGAGC GACAGCCATC
    AACGCCAGCA GAGCTCTGAA ATCCCTCATC CCTCTATACC AAAACTTCTA TGAAGAGAGG
    GCCAGATATC TGGAGACGAT AGTCCAACAT CACTTGGAGC CCACGACATT TGAGGACTAC
    GCCGCTCGTG TCTTTTGTCC TGCGCCTTTC CACTATCTTC CGTCTGAAGC AGAGAGAAAT
    GCTGTGATTT TTCTGCCCCT CCCTAAACCT CCACTAGAAA ACATTGGGCT CACCAAGGTA
    AAGGGCAAAG GCTTGAAACG GAAGGTCTTT CACGCTAGCT GTGAATGGCT CATGCCTCGA
    GAGTCAGCTG GCAGAAAGTC CAGCAATGCA GGTCGACGGG AGCGACTTAG CCTCGCCAAT
    GTCACCTCGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149654
    Clone ID Related Accession (Same CDS sequence) XM_021467316.1
    Accession Version XM_021467316.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGA CCTGGAGGAG
    CCTCCTATGA TCCAGCGTGG GCCACGGAAC CGCCACTGCT CTCAGAGCGA GGAGACGCCG
    ACGTCTGAAG TTTTTTCGGC CTCCGGTGAA CTGGAGCAGC CTCCGCTTCC CCGCAGTAGC
    AGTGCCTCCG ACATCATGGA GCCCTTCATC GCAGAACGGG CCAAAGCCAA TAAAGACGAG
    CTGACGCCGA AGATGCGGCC CATGTCTAAC GATGCTGGCA GCAGCAACCC CAATGTCAGT
    GACCTCATGG ACGAGTTCAT CCAGGAGAGA CTGAGAGCCA GAGGCAATTC TGGCATGACG
    AGGCGCGGCA GTAGCCCAGG AAGTCTGGAG ATCCCTAAAG ATCTGCCAGA GCTGCTGAAC
    CGTCAGAACG CCACACGTCC CGCAGACGAC CCCGGTGTGC CCTCTGAGTG GACCTCGCCT
    GCCAGTGCCA GTGGAAGTGA CCTCATAAGC TCTGACAGCC AATCGGATTC CTTTAACGCT
    TTTCAATATG ACAGCAAATT CGAAAATTTC AGCTTCCCCC CTGAGACGTG TGCTTTGGCG
    TCTGCAGATC AGGATAGTTT GAGTGGGACA GGACAGACAG CGGAGGAACA GGAGCTTTCC
    AGTCTCACAA CACTTCACAT CGACTCTGAG ACCAGCAGCC TTAGTCAGCA CGGCCTGTCT
    GCTGATACTG TCACTATTAC TGGCTCTGAG AGCGCATCTC CAATGCACTC TCTAGGAGGA
    TCTCGATCGC AGACGCCATC TCCTGCAACC CTGACCGCTG AACACGTCCA GCATAAAGAG
    CTGCAGCTGG ATGAGAAGAT GCACCATTCT GTTCTGCAGA CCCCTGATGA CCTTGAAACC
    AGCGAGTTTC CCACTGAGGA CTGTAGTGTA ATGGCTGGTG GCACTCTGAC TGGCTGGCAT
    GCTGATGTCG CAACAGTAAT GTGGAGGAGA ATGCTTGGGA TTCTGGGAGA TGTCAACTAT
    ATAAAGGACC CTGAGATCCA TGCGCAGGTT TTTGACTACC TCTGCGAACT CTGGCAGAAC
    CTGGCCAAGA TCAGAGACAA TCTCGGGATC TCCCTTGACA ACCAGTCGTC TCCGCCACCA
    CCAGATCTGA TTCCACCTCT GCGGATTCTG ACTCCATGGC TTTTCAAAGC GACTATGTTG
    GGCGAACGCT ACAAGCAGGG AAAACTGCAC GCCTACAAGC TCATCTGTAA GATCATGAAG
    AGAAGACAGG ACGTTTCCCC AAACTCTGAC TTCCTCACGC ACTTCTACAA CATCATGCAC
    TGCGGCCTGC TCCACCAAGA CCAGGATATT GTGAACACCA TCATTAAGCA CTGCTCTCCA
    CGCTTCTTCT CTATTGGACT TCCAGGAGCC ACAATGCTCA TCCTGGACTT CATCGTGGCA
    GCTAGTCGTG TCACTGCCTG CTCGTCCCTC AATGCTCCAC GGGTGGAGGC TCAGATCTTG
    TTGGGTTCCC TGGTTTGCCT CCCAAACTTG TACAATGAAC TTCCTGCACT GCATCCTACA
    ACAGCCGACA TCATCATGAC CAAATTCACT GATGTCAAGG AGCATATAAT CAAGCACATC
    CTGACCTCTG CCAGAGATGA GCCTTCTGCT CCAGCCAGGT GTGTGGCGCT CTGTAGTCTG
    GGTATTTGGC TGTGTGAAGA GCTGGTTCAT GGCACTCAGC ATCCGCAGAT CAAAGAAGCG
    CTTAACGTCA TCTGTGTCAC TCTCAAGTTC TCCAATAAAA ACGTCGCCCT GGTGGCTTCA
    GACATTTTGC ACCTGCTGAT AAACTACGTG GATGAACTGG AGAAATTTCC ACCCAACACT
    CCAAAGAAAA TAGTGGAGGT TCTTATTGCA ACCATCACTT ACCTTCTTCC AGCCACAGAG
    TCCTCTCCTC ATGAGCTGGA CAAGAGGCTG GTTGTTTCTC TACTGCTGTG CTTGCTGGAC
    TGGGTGATGG CTCTACCTCC GAAAACACTC CTCCAGCCGG TCCAAGGAGC CTCGGATAAG
    GAGAAAACTG ACAAGTCTGT CCTCAGCTGC ATCTATAAAG TCCTCCATGG TTGTGTCTAT
    GGGGCTCAGA GCTTCAGTAA CCCCAGTTAC TTCCCCATTC ACCTGTCAGA CTTAAGCAGC
    CCAGACTACG ACCCCTTCTT ACTACTAGAA AACCTTAAGG AGCCGGAGCC ACTCCATTCA
    CCTGACTCTG AACGCTCCTC CAAACTGCAA CCAGTAACTG AAGTGAGGAG CCGCATTCAG
    CAAGGCCTTG TTTCAGTTGC GGCACGCACT GTCATATCTC ACCTAGTCAA CCATCTGGGT
    CACTATCCCA TGAGTGGTGG TCCGGCCACT CTGACGAGCC AGGTGTGCGA GAACCAGGAC
    AACCCATACA GTGAGAGCGC AGACCTGACC CCTGAACTCT TTGACGCCCC CAATTTGCAG
    TTCTTCGTGC TTAATGGCAC CACCCTGCTG TCCTACTTAC AAATTCGGGC TGAAGATGGC
    CTGCCCGGTG GTGGGATGTC AGCTGGCCTC ACCACCACCA ATGCTTGTGT TCGGGTGATT
    GTGAGGGACA TTTCTGGTAA ACACTCCTGG GACTCAGCTG TTCTGTATGG TCCACCTCAT
    TGCATCACTC CTAGTCAAAC TTTGCACCTT TACACGTCCT CTAATCCGAG AGGGGAACAT
    AATAGAGATG AGGACAGTTT AGAGAGGGGC AGCCAAGAAG AACCAGAGGA TGGAGCTTTT
    CAGGAAAGCG AGGAGGACTT GGATGAGCAT GAGGAGGCGG AGGTGAAAGA AGACGAGGTG
    GAGGAGGAAG AACTTGAGGA AGAGATTGAG GAGGAAGAGG TTGCACTGGA ACTTATGGCC
    CCGCAGGCCA AACGGCGGTG CAGAGAGGTC ATCCCGAACT GGGACTCCCT GCAGGATGGG
    GAAGACTCCC TAGATGAGAT GCTGCAATAT CTGGGATACT CCAGCCCAGA GTGTCTGCAA
    CGTACTGGTG GACCTCTCAA CATACCTGCC CCACCTCCAA ACTGTGTGTC CGAGAAACAG
    GAGAATGATG TCATCAATGC CATCCTGAAG CAGTGTGCCG AGGAGCAGGA CTTCACAATT
    CACCGTGGTA ATGAGCTGAA CATGAGGGCC GTCGTTCAGC AGGAGCCAAG CCCACAGAGA
    CCACAGTCTG CCTTCTACTA CTGCAAACTG CTCCTCAACA TACTAGGCAT GAACTCTTGG
    GAGAAGAGGA ACAGTTTCCA CCTGCTGAAA AAGAATGAAA AACTACTGAG AGAGCTGAAG
    AATCTGGACT CCAGGCAGTG TCGTGAAACA CACAAAATCG CGGTGTTTTA CGTGGCAGAG
    GGACAAGAAG ACAAGCACTC CATTCTGTCC AACACAGCCG GGAGTCAGGC GTATGAAGAC
    TTTGTATCAG GCCTGGGCTG GGAGGTGAAC CTGACGAGTC ACTGTGGCTT TATGGGTGGC
    CTGCAGCGGA ACAAGAGCAC GGGTTTTACC ATGCCTTACT TTGCCACCTC TACAGTGGAA
    GTGATGTTCC ACGTCTCCAC ACGCATGCCT CCAGACTCAG ACGACTCGCT CACTAAAAAG
    TTGAGACACC TGGGTAATGA TGAAGTGCAC ATCGTGTGGT CTGAGCACTC GCGGGACTAT
    CGGAGAGGCG TCATTCCTAC TGAGTTTGGA GACGTGCTCA TCGTCATCTA CCCTATGAAG
    AACCACATGT ACAGCATCCA GATCATCAAG AAACCTGAGG TGCCGTTCTT TGGTCCCTTG
    TTTGACGGAG CCATAGTGGA TGGGACTATA TTGCCCACAG TGGTGCGAGC GACAGCCATC
    AACGCCAGCA GAGCTCTGAA ATCCCTCATC CCTCTATACC AAAACTTCTA TGAAGAGAGG
    GCCAGATATC TGGAGACGAT AGTCCAACAT CACTTGGAGC CCACGACATT TGAGGACTAC
    GCCGCTCGTG TCTTTTGTCC TGCGCCTTTC CACTATCTTC CGTCTGAAGC AGGCTCATGC
    CTCGAGAGTC AGCTGGCAGA AAGTCCAGCA ATGCAGGTCG ACGGGAGCGA CTTAGCCTCG
    CCAATGTCAC CTCGTGCTAG CAAGAGCCGG ATGTCCATGA AGCTCCGCCG CTCCTCGGGC
    TCCGCCAACA AGACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322991.1
    CDS64..6381
    Translation

    Target ORF information:

    RefSeq Version XM_021467316.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGA CCTGGAGGAG
    CCTCCTATGA TCCAGCGTGG GCCACGGAAC CGCCACTGCT CTCAGAGCGA GGAGACGCCG
    ACGTCTGAAG TTTTTTCGGC CTCCGGTGAA CTGGAGCAGC CTCCGCTTCC CCGCAGTAGC
    AGTGCCTCCG ACATCATGGA GCCCTTCATC GCAGAACGGG CCAAAGCCAA TAAAGACGAG
    CTGACGCCGA AGATGCGGCC CATGTCTAAC GATGCTGGCA GCAGCAACCC CAATGTCAGT
    GACCTCATGG ACGAGTTCAT CCAGGAGAGA CTGAGAGCCA GAGGCAATTC TGGCATGACG
    AGGCGCGGCA GTAGCCCAGG AAGTCTGGAG ATCCCTAAAG ATCTGCCAGA GCTGCTGAAC
    CGTCAGAACG CCACACGTCC CGCAGACGAC CCCGGTGTGC CCTCTGAGTG GACCTCGCCT
    GCCAGTGCCA GTGGAAGTGA CCTCATAAGC TCTGACAGCC AATCGGATTC CTTTAACGCT
    TTTCAATATG ACAGCAAATT CGAAAATTTC AGCTTCCCCC CTGAGACGTG TGCTTTGGCG
    TCTGCAGATC AGGATAGTTT GAGTGGGACA GGACAGACAG CGGAGGAACA GGAGCTTTCC
    AGTCTCACAA CACTTCACAT CGACTCTGAG ACCAGCAGCC TTAGTCAGCA CGGCCTGTCT
    GCTGATACTG TCACTATTAC TGGCTCTGAG AGCGCATCTC CAATGCACTC TCTAGGAGGA
    TCTCGATCGC AGACGCCATC TCCTGCAACC CTGACCGCTG AACACGTCCA GCATAAAGAG
    CTGCAGCTGG ATGAGAAGAT GCACCATTCT GTTCTGCAGA CCCCTGATGA CCTTGAAACC
    AGCGAGTTTC CCACTGAGGA CTGTAGTGTA ATGGCTGGTG GCACTCTGAC TGGCTGGCAT
    GCTGATGTCG CAACAGTAAT GTGGAGGAGA ATGCTTGGGA TTCTGGGAGA TGTCAACTAT
    ATAAAGGACC CTGAGATCCA TGCGCAGGTT TTTGACTACC TCTGCGAACT CTGGCAGAAC
    CTGGCCAAGA TCAGAGACAA TCTCGGGATC TCCCTTGACA ACCAGTCGTC TCCGCCACCA
    CCAGATCTGA TTCCACCTCT GCGGATTCTG ACTCCATGGC TTTTCAAAGC GACTATGTTG
    GGCGAACGCT ACAAGCAGGG AAAACTGCAC GCCTACAAGC TCATCTGTAA GATCATGAAG
    AGAAGACAGG ACGTTTCCCC AAACTCTGAC TTCCTCACGC ACTTCTACAA CATCATGCAC
    TGCGGCCTGC TCCACCAAGA CCAGGATATT GTGAACACCA TCATTAAGCA CTGCTCTCCA
    CGCTTCTTCT CTATTGGACT TCCAGGAGCC ACAATGCTCA TCCTGGACTT CATCGTGGCA
    GCTAGTCGTG TCACTGCCTG CTCGTCCCTC AATGCTCCAC GGGTGGAGGC TCAGATCTTG
    TTGGGTTCCC TGGTTTGCCT CCCAAACTTG TACAATGAAC TTCCTGCACT GCATCCTACA
    ACAGCCGACA TCATCATGAC CAAATTCACT GATGTCAAGG AGCATATAAT CAAGCACATC
    CTGACCTCTG CCAGAGATGA GCCTTCTGCT CCAGCCAGGT GTGTGGCGCT CTGTAGTCTG
    GGTATTTGGC TGTGTGAAGA GCTGGTTCAT GGCACTCAGC ATCCGCAGAT CAAAGAAGCG
    CTTAACGTCA TCTGTGTCAC TCTCAAGTTC TCCAATAAAA ACGTCGCCCT GGTGGCTTCA
    GACATTTTGC ACCTGCTGAT AAACTACGTG GATGAACTGG AGAAATTTCC ACCCAACACT
    CCAAAGAAAA TAGTGGAGGT TCTTATTGCA ACCATCACTT ACCTTCTTCC AGCCACAGAG
    TCCTCTCCTC ATGAGCTGGA CAAGAGGCTG GTTGTTTCTC TACTGCTGTG CTTGCTGGAC
    TGGGTGATGG CTCTACCTCC GAAAACACTC CTCCAGCCGG TCCAAGGAGC CTCGGATAAG
    GAGAAAACTG ACAAGTCTGT CCTCAGCTGC ATCTATAAAG TCCTCCATGG TTGTGTCTAT
    GGGGCTCAGA GCTTCAGTAA CCCCAGTTAC TTCCCCATTC ACCTGTCAGA CTTAAGCAGC
    CCAGACTACG ACCCCTTCTT ACTACTAGAA AACCTTAAGG AGCCGGAGCC ACTCCATTCA
    CCTGACTCTG AACGCTCCTC CAAACTGCAA CCAGTAACTG AAGTGAGGAG CCGCATTCAG
    CAAGGCCTTG TTTCAGTTGC GGCACGCACT GTCATATCTC ACCTAGTCAA CCATCTGGGT
    CACTATCCCA TGAGTGGTGG TCCGGCCACT CTGACGAGCC AGGTGTGCGA GAACCAGGAC
    AACCCATACA GTGAGAGCGC AGACCTGACC CCTGAACTCT TTGACGCCCC CAATTTGCAG
    TTCTTCGTGC TTAATGGCAC CACCCTGCTG TCCTACTTAC AAATTCGGGC TGAAGATGGC
    CTGCCCGGTG GTGGGATGTC AGCTGGCCTC ACCACCACCA ATGCTTGTGT TCGGGTGATT
    GTGAGGGACA TTTCTGGTAA ACACTCCTGG GACTCAGCTG TTCTGTATGG TCCACCTCAT
    TGCATCACTC CTAGTCAAAC TTTGCACCTT TACACGTCCT CTAATCCGAG AGGGGAACAT
    AATAGAGATG AGGACAGTTT AGAGAGGGGC AGCCAAGAAG AACCAGAGGA TGGAGCTTTT
    CAGGAAAGCG AGGAGGACTT GGATGAGCAT GAGGAGGCGG AGGTGAAAGA AGACGAGGTG
    GAGGAGGAAG AACTTGAGGA AGAGATTGAG GAGGAAGAGG TTGCACTGGA ACTTATGGCC
    CCGCAGGCCA AACGGCGGTG CAGAGAGGTC ATCCCGAACT GGGACTCCCT GCAGGATGGG
    GAAGACTCCC TAGATGAGAT GCTGCAATAT CTGGGATACT CCAGCCCAGA GTGTCTGCAA
    CGTACTGGTG GACCTCTCAA CATACCTGCC CCACCTCCAA ACTGTGTGTC CGAGAAACAG
    GAGAATGATG TCATCAATGC CATCCTGAAG CAGTGTGCCG AGGAGCAGGA CTTCACAATT
    CACCGTGGTA ATGAGCTGAA CATGAGGGCC GTCGTTCAGC AGGAGCCAAG CCCACAGAGA
    CCACAGTCTG CCTTCTACTA CTGCAAACTG CTCCTCAACA TACTAGGCAT GAACTCTTGG
    GAGAAGAGGA ACAGTTTCCA CCTGCTGAAA AAGAATGAAA AACTACTGAG AGAGCTGAAG
    AATCTGGACT CCAGGCAGTG TCGTGAAACA CACAAAATCG CGGTGTTTTA CGTGGCAGAG
    GGACAAGAAG ACAAGCACTC CATTCTGTCC AACACAGCCG GGAGTCAGGC GTATGAAGAC
    TTTGTATCAG GCCTGGGCTG GGAGGTGAAC CTGACGAGTC ACTGTGGCTT TATGGGTGGC
    CTGCAGCGGA ACAAGAGCAC GGGTTTTACC ATGCCTTACT TTGCCACCTC TACAGTGGAA
    GTGATGTTCC ACGTCTCCAC ACGCATGCCT CCAGACTCAG ACGACTCGCT CACTAAAAAG
    TTGAGACACC TGGGTAATGA TGAAGTGCAC ATCGTGTGGT CTGAGCACTC GCGGGACTAT
    CGGAGAGGCG TCATTCCTAC TGAGTTTGGA GACGTGCTCA TCGTCATCTA CCCTATGAAG
    AACCACATGT ACAGCATCCA GATCATCAAG AAACCTGAGG TGCCGTTCTT TGGTCCCTTG
    TTTGACGGAG CCATAGTGGA TGGGACTATA TTGCCCACAG TGGTGCGAGC GACAGCCATC
    AACGCCAGCA GAGCTCTGAA ATCCCTCATC CCTCTATACC AAAACTTCTA TGAAGAGAGG
    GCCAGATATC TGGAGACGAT AGTCCAACAT CACTTGGAGC CCACGACATT TGAGGACTAC
    GCCGCTCGTG TCTTTTGTCC TGCGCCTTTC CACTATCTTC CGTCTGAAGC AGGCTCATGC
    CTCGAGAGTC AGCTGGCAGA AAGTCCAGCA ATGCAGGTCG ACGGGAGCGA CTTAGCCTCG
    CCAATGTCAC CTCGTGCTAG CAAGAGCCGG ATGTCCATGA AGCTCCGCCG CTCCTCGGGC
    TCCGCCAACA AGACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149655
    Clone ID Related Accession (Same CDS sequence) XM_021467317.1
    Accession Version XM_021467317.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGA CCTGGAGGAG
    CCTCCTATGA TCCAGCGTGG GCCACGGAAC CGCCACTGCT CTCAGAGCGA GGAGACGCCG
    ACGTCTGAAG TTTTTTCGGC CTCCGGTGAA CTGGAGCAGC CTCCGCTTCC CCGCAGTAGC
    AGTGCCTCCG ACATCATGGA GCCCTTCATC GCAGAACGGG CCAAAGCCAA TAAAGACGAG
    CTGACGCCGA AGATGCGGCC CATGTCTAAC GATGCTGGCA GCAGCAACCC CAATGTCAGT
    GACCTCATGG ACGAGTTCAT CCAGGAGAGA CTGAGAGCCA GAGGCAATTC TGGCATGACG
    AGGCGCGGCA GTAGCCCAGG AAGTCTGGAG ATCCCTAAAG ATCTGCCAGA GCTGCTGAAC
    CGTCAGAACG CCACACGTCC CGCAGACGAC CCCGGTGTGC CCTCTGAGTG GACCTCGCCT
    GCCAGTGCCA GTGGAAGTGA CCTCATAAGC TCTGACAGCC AATCGGATTC CTTTAACGCT
    TTTCAATATG ACAGCAAATT CGAAAATTTC AGCTTCCCCC CTGAGACGTG TGCTTTGGCG
    TCTGCAGATC AGGATAGTTT GAGTGGGACA GGACAGACAG CGGAGGAACA GGAGCTTTCC
    AGTCTCACAA CACTTCACAT CGACTCTGAG ACCAGCAGCC TTAGTCAGCA CGGCCTGTCT
    GCTGATACTG TCACTATTAC TGGCTCTGAG AGCGCATCTC CAATGCACTC TCTAGGAGGA
    TCTCGATCGC AGACGCCATC TCCTGCAACC CTGACCGCTG AACACGTCCA GCATAAAGAG
    CTGCAGCTGG ATGAGAAGAT GCACCATTCT GTTCTGCAGA CCCCTGATGA CCTTGAAACC
    AGCGAGTTTC CCACTGAGGA CTGTAGTGTA ATGGCTGGTG GCACTCTGAC TGGCTGGCAT
    GCTGATGTCG CAACAGTAAT GTGGAGGAGA ATGCTTGGGA TTCTGGGAGA TGTCAACTAT
    ATAAAGGACC CTGAGATCCA TGCGCAGGTT TTTGACTACC TCTGCGAACT CTGGCAGAAC
    CTGGCCAAGA TCAGAGACAA TCTCGGGATC TCCCTTGACA ACCAGTCGTC TCCGCCACCA
    CCAGATCTGA TTCCACCTCT GCGGATTCTG ACTCCATGGC TTTTCAAAGC GACTATGTTG
    GGCGAACGCT ACAAGCAGGG AAAACTGCAC GCCTACAAGC TCATCTGTAA GATCATGAAG
    AGAAGACAGG ACGTTTCCCC AAACTCTGAC TTCCTCACGC ACTTCTACAA CATCATGCAC
    TGCGGCCTGC TCCACCAAGA CCAGGATATT GTGAACACCA TCATTAAGCA CTGCTCTCCA
    CGCTTCTTCT CTATTGGACT TCCAGGAGCC ACAATGCTCA TCCTGGACTT CATCGTGGCA
    GCTAGTCGTG TCACTGCCTG CTCGTCCCTC AATGCTCCAC GGGTGGAGGC TCAGATCTTG
    TTGGGTTCCC TGGTTTGCCT CCCAAACTTG TACAATGAAC TTCCTGCACT GCATCCTACA
    ACAGCCGACA TCATCATGAC CAAATTCACT GATGTCAAGG AGCATATAAT CAAGCACATC
    CTGACCTCTG CCAGAGATGA GCCTTCTGCT CCAGCCAGGT GTGTGGCGCT CTGTAGTCTG
    GGTATTTGGC TGTGTGAAGA GCTGGTTCAT GGCACTCAGC ATCCGCAGAT CAAAGAAGCG
    CTTAACGTCA TCTGTGTCAC TCTCAAGTTC TCCAATAAAA ACGTCGCCCT GGTGGCTTCA
    GACATTTTGC ACCTGCTGAT AAACTACGTG GATGAACTGG AGAAATTTCC ACCCAACACT
    CCAAAGAAAA TAGTGGAGGT TCTTATTGCA ACCATCACTT ACCTTCTTCC AGCCACAGAG
    TCCTCTCCTC ATGAGCTGGA CAAGAGGCTG GTTGTTTCTC TACTGCTGTG CTTGCTGGAC
    TGGGTGATGG CTCTACCTCC GAAAACACTC CTCCAGCCGG TCCAAGGAGC CTCGGATAAG
    GAGAAAACTG ACAAGTCTGT CCTCAGCTGC ATCTATAAAG TCCTCCATGG TTGTGTCTAT
    GGGGCTCAGA GCTTCAGTAA CCCCAGTTAC TTCCCCATTC ACCTGTCAGA CTTAAGCAGC
    CCAGACTACG ACCCCTTCTT ACTACTAGAA AACCTTAAGG AGCCGGAGCC ACTCCATTCA
    CCTGACTCTG AACGCTCCTC CAAACTGCAA CCAGTAACTG AAGTGAGGAG CCGCATTCAG
    CAAGGCCTTG TTTCAGTTGC GGCACGCACT GTCATATCTC ACCTAGTCAA CCATCTGGGT
    CACTATCCCA TGAGTGGTGG TCCGGCCACT CTGACGAGCC AGGTGTGCGA GAACCAGGAC
    AACCCATACA GTGAGAGCGC AGACCTGACC CCTGAACTCT TTGACGCCCC CAATTTGCAG
    TTCTTCGTGC TTAATGGCAC CACCCTGCTG TCCTACTTAC AAATTCGGGC TGAAGATGGC
    CTGCCCGGTG GTGGGATGTC AGCTGGCCTC ACCACCACCA ATGCTTGTGT TCGGGTGATT
    GTGAGGGACA TTTCTGGTAA ACACTCCTGG GACTCAGCTG TTCTGTATGG TCCACCTCAT
    TGCATCACTC CTAGTCAAAC TTTGCACCTT TACACGTCCT CTAATCCGAG AGGGGAACAT
    AATAGAGATG AGGACAGTTT AGAGAGGGGC AGCCAAGAAG AACCAGAGGA TGGAGCTTTT
    CAGGAAAGCG AGGAGGACTT GGATGAGCAT GAGGAGGCGG AGGTGAAAGA AGACGAGGTG
    GAGGAGGAAG AACTTGAGGA AGAGATTGAG GAGGAAGAGG TTGCACTGGA ACTTATGGCC
    CCGCAGGCCA AACGGCGGTG CAGAGAGGTC ATCCCGAACT GGGACTCCCT GCAGGATGGG
    GAAGACTCCC TAGATGAGAT GCTGCAATAT CTGGGATACT CCAGCCCAGA GTGTCTGCAA
    CGTACTGGTG GACCTCTCAA CATACCTGCC CCACCTCCAA ACTGTGTGTC CGAGAAACAG
    GAGAATGATG TCATCAATGC CATCCTGAAG CAGTGTGCCG AGGAGCAGGA CTTCACAATT
    CACCGTGGTA ATGAGCTGAA CATGAGGGCC GTCGTTCAGC AGGAGCCAAG CCCACAGAGA
    CCACAGTCTG CCTTCTACTA CTGCAAACTG CTCCTCAACA TACTAGGCAT GAACTCTTGG
    GAGAAGAGGA ACAGTTTCCA CCTGCTGAAA AAGAATGAAA AACTACTGAG AGAGCTGAAG
    AATCTGGACT CCAGGCAGTG TCGTGAAACA CACAAAATCG CGGTGTTTTA CGTGGCAGAG
    GGACAAGAAG ACAAGCACTC CATTCTGTCC AACACAGCCG GGAGTCAGGC GTATGAAGAC
    TTTGTATCAG GCCTGGGCTG GGAGGTGAAC CTGACGAGTC ACTGTGGCTT TATGGGTGGC
    CTGCAGCGGA ACAAGAGCAC GGGTTTTACC ATGCCTTACT TTGCCACCTC TACAGTGGAA
    GTGATGTTCC ACGTCTCCAC ACGCATGCCT CCAGACTCAG ACGACTCGCT CACTAAAAAG
    TTGAGACACC TGGGTAATGA TGAAGTGCAC ATCGTGTGGT CTGAGCACTC GCGGGACTAT
    CGGAGAGGCG TCATTCCTAC TGAGTTTGGA GACGTGCTCA TCGTCATCTA CCCTATGAAG
    AACCACATGT ACAGCATCCA GATCATCAAG AAACCTGAGG TGCCGTTCTT TGGTCCCTTG
    TTTGACGGAG CCATAGTGGA TGGGACTATA TTGCCCACAG TGGTGCGAGC GACAGCCATC
    AACGCCAGCA GAGCTCTGAA ATCCCTCATC CCTCTATACC AAAACTTCTA TGAAGAGAGG
    GCCAGATATC TGGAGACGAT AGTCCAACAT CACTTGGAGC CCACGACATT TGAGGACTAC
    GCCGCTCGTG TCTTTTGTCC TGCGCCTTTC CACTATCTTC CGTCTGAAGC AGATCATAAT
    CTATCACTTG TGTATATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322992.1
    CDS64..6264
    Translation

    Target ORF information:

    RefSeq Version XM_021467317.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467317.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGA CCTGGAGGAG
    CCTCCTATGA TCCAGCGTGG GCCACGGAAC CGCCACTGCT CTCAGAGCGA GGAGACGCCG
    ACGTCTGAAG TTTTTTCGGC CTCCGGTGAA CTGGAGCAGC CTCCGCTTCC CCGCAGTAGC
    AGTGCCTCCG ACATCATGGA GCCCTTCATC GCAGAACGGG CCAAAGCCAA TAAAGACGAG
    CTGACGCCGA AGATGCGGCC CATGTCTAAC GATGCTGGCA GCAGCAACCC CAATGTCAGT
    GACCTCATGG ACGAGTTCAT CCAGGAGAGA CTGAGAGCCA GAGGCAATTC TGGCATGACG
    AGGCGCGGCA GTAGCCCAGG AAGTCTGGAG ATCCCTAAAG ATCTGCCAGA GCTGCTGAAC
    CGTCAGAACG CCACACGTCC CGCAGACGAC CCCGGTGTGC CCTCTGAGTG GACCTCGCCT
    GCCAGTGCCA GTGGAAGTGA CCTCATAAGC TCTGACAGCC AATCGGATTC CTTTAACGCT
    TTTCAATATG ACAGCAAATT CGAAAATTTC AGCTTCCCCC CTGAGACGTG TGCTTTGGCG
    TCTGCAGATC AGGATAGTTT GAGTGGGACA GGACAGACAG CGGAGGAACA GGAGCTTTCC
    AGTCTCACAA CACTTCACAT CGACTCTGAG ACCAGCAGCC TTAGTCAGCA CGGCCTGTCT
    GCTGATACTG TCACTATTAC TGGCTCTGAG AGCGCATCTC CAATGCACTC TCTAGGAGGA
    TCTCGATCGC AGACGCCATC TCCTGCAACC CTGACCGCTG AACACGTCCA GCATAAAGAG
    CTGCAGCTGG ATGAGAAGAT GCACCATTCT GTTCTGCAGA CCCCTGATGA CCTTGAAACC
    AGCGAGTTTC CCACTGAGGA CTGTAGTGTA ATGGCTGGTG GCACTCTGAC TGGCTGGCAT
    GCTGATGTCG CAACAGTAAT GTGGAGGAGA ATGCTTGGGA TTCTGGGAGA TGTCAACTAT
    ATAAAGGACC CTGAGATCCA TGCGCAGGTT TTTGACTACC TCTGCGAACT CTGGCAGAAC
    CTGGCCAAGA TCAGAGACAA TCTCGGGATC TCCCTTGACA ACCAGTCGTC TCCGCCACCA
    CCAGATCTGA TTCCACCTCT GCGGATTCTG ACTCCATGGC TTTTCAAAGC GACTATGTTG
    GGCGAACGCT ACAAGCAGGG AAAACTGCAC GCCTACAAGC TCATCTGTAA GATCATGAAG
    AGAAGACAGG ACGTTTCCCC AAACTCTGAC TTCCTCACGC ACTTCTACAA CATCATGCAC
    TGCGGCCTGC TCCACCAAGA CCAGGATATT GTGAACACCA TCATTAAGCA CTGCTCTCCA
    CGCTTCTTCT CTATTGGACT TCCAGGAGCC ACAATGCTCA TCCTGGACTT CATCGTGGCA
    GCTAGTCGTG TCACTGCCTG CTCGTCCCTC AATGCTCCAC GGGTGGAGGC TCAGATCTTG
    TTGGGTTCCC TGGTTTGCCT CCCAAACTTG TACAATGAAC TTCCTGCACT GCATCCTACA
    ACAGCCGACA TCATCATGAC CAAATTCACT GATGTCAAGG AGCATATAAT CAAGCACATC
    CTGACCTCTG CCAGAGATGA GCCTTCTGCT CCAGCCAGGT GTGTGGCGCT CTGTAGTCTG
    GGTATTTGGC TGTGTGAAGA GCTGGTTCAT GGCACTCAGC ATCCGCAGAT CAAAGAAGCG
    CTTAACGTCA TCTGTGTCAC TCTCAAGTTC TCCAATAAAA ACGTCGCCCT GGTGGCTTCA
    GACATTTTGC ACCTGCTGAT AAACTACGTG GATGAACTGG AGAAATTTCC ACCCAACACT
    CCAAAGAAAA TAGTGGAGGT TCTTATTGCA ACCATCACTT ACCTTCTTCC AGCCACAGAG
    TCCTCTCCTC ATGAGCTGGA CAAGAGGCTG GTTGTTTCTC TACTGCTGTG CTTGCTGGAC
    TGGGTGATGG CTCTACCTCC GAAAACACTC CTCCAGCCGG TCCAAGGAGC CTCGGATAAG
    GAGAAAACTG ACAAGTCTGT CCTCAGCTGC ATCTATAAAG TCCTCCATGG TTGTGTCTAT
    GGGGCTCAGA GCTTCAGTAA CCCCAGTTAC TTCCCCATTC ACCTGTCAGA CTTAAGCAGC
    CCAGACTACG ACCCCTTCTT ACTACTAGAA AACCTTAAGG AGCCGGAGCC ACTCCATTCA
    CCTGACTCTG AACGCTCCTC CAAACTGCAA CCAGTAACTG AAGTGAGGAG CCGCATTCAG
    CAAGGCCTTG TTTCAGTTGC GGCACGCACT GTCATATCTC ACCTAGTCAA CCATCTGGGT
    CACTATCCCA TGAGTGGTGG TCCGGCCACT CTGACGAGCC AGGTGTGCGA GAACCAGGAC
    AACCCATACA GTGAGAGCGC AGACCTGACC CCTGAACTCT TTGACGCCCC CAATTTGCAG
    TTCTTCGTGC TTAATGGCAC CACCCTGCTG TCCTACTTAC AAATTCGGGC TGAAGATGGC
    CTGCCCGGTG GTGGGATGTC AGCTGGCCTC ACCACCACCA ATGCTTGTGT TCGGGTGATT
    GTGAGGGACA TTTCTGGTAA ACACTCCTGG GACTCAGCTG TTCTGTATGG TCCACCTCAT
    TGCATCACTC CTAGTCAAAC TTTGCACCTT TACACGTCCT CTAATCCGAG AGGGGAACAT
    AATAGAGATG AGGACAGTTT AGAGAGGGGC AGCCAAGAAG AACCAGAGGA TGGAGCTTTT
    CAGGAAAGCG AGGAGGACTT GGATGAGCAT GAGGAGGCGG AGGTGAAAGA AGACGAGGTG
    GAGGAGGAAG AACTTGAGGA AGAGATTGAG GAGGAAGAGG TTGCACTGGA ACTTATGGCC
    CCGCAGGCCA AACGGCGGTG CAGAGAGGTC ATCCCGAACT GGGACTCCCT GCAGGATGGG
    GAAGACTCCC TAGATGAGAT GCTGCAATAT CTGGGATACT CCAGCCCAGA GTGTCTGCAA
    CGTACTGGTG GACCTCTCAA CATACCTGCC CCACCTCCAA ACTGTGTGTC CGAGAAACAG
    GAGAATGATG TCATCAATGC CATCCTGAAG CAGTGTGCCG AGGAGCAGGA CTTCACAATT
    CACCGTGGTA ATGAGCTGAA CATGAGGGCC GTCGTTCAGC AGGAGCCAAG CCCACAGAGA
    CCACAGTCTG CCTTCTACTA CTGCAAACTG CTCCTCAACA TACTAGGCAT GAACTCTTGG
    GAGAAGAGGA ACAGTTTCCA CCTGCTGAAA AAGAATGAAA AACTACTGAG AGAGCTGAAG
    AATCTGGACT CCAGGCAGTG TCGTGAAACA CACAAAATCG CGGTGTTTTA CGTGGCAGAG
    GGACAAGAAG ACAAGCACTC CATTCTGTCC AACACAGCCG GGAGTCAGGC GTATGAAGAC
    TTTGTATCAG GCCTGGGCTG GGAGGTGAAC CTGACGAGTC ACTGTGGCTT TATGGGTGGC
    CTGCAGCGGA ACAAGAGCAC GGGTTTTACC ATGCCTTACT TTGCCACCTC TACAGTGGAA
    GTGATGTTCC ACGTCTCCAC ACGCATGCCT CCAGACTCAG ACGACTCGCT CACTAAAAAG
    TTGAGACACC TGGGTAATGA TGAAGTGCAC ATCGTGTGGT CTGAGCACTC GCGGGACTAT
    CGGAGAGGCG TCATTCCTAC TGAGTTTGGA GACGTGCTCA TCGTCATCTA CCCTATGAAG
    AACCACATGT ACAGCATCCA GATCATCAAG AAACCTGAGG TGCCGTTCTT TGGTCCCTTG
    TTTGACGGAG CCATAGTGGA TGGGACTATA TTGCCCACAG TGGTGCGAGC GACAGCCATC
    AACGCCAGCA GAGCTCTGAA ATCCCTCATC CCTCTATACC AAAACTTCTA TGAAGAGAGG
    GCCAGATATC TGGAGACGAT AGTCCAACAT CACTTGGAGC CCACGACATT TGAGGACTAC
    GCCGCTCGTG TCTTTTGTCC TGCGCCTTTC CACTATCTTC CGTCTGAAGC AGATCATAAT
    CTATCACTTG TGTATATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149656
    Clone ID Related Accession (Same CDS sequence) XM_021467318.1
    Accession Version XM_021467318.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGCCA ATAAAGACGA GCTGACGCCG AAGATGCGGC CCATGTCTAA CGATGCTGGC
    AGCAGCAACC CCAATGTCAG TGACCTCATG GACGAGTTCA TCCAGGAGAG ACTGAGAGCC
    AGAGGCAATT CTGGCATGAC GAGGCGCGGC AGTAGCCCAG GAAGTCTGGA GATCCCTAAA
    GATCTGCCAG AGCTGCTGAA CCGTCAGAAC GCCACACGTC CCGCAGACGA CCCCGGTGTG
    CCCTCTGAGT GGACCTCGCC TGCCAGTGCC AGTGGAAGTG ACCTCATAAG CTCTGACAGC
    CAATCGGATT CCTTTAACGC TTTTCAATAT GACAGCAAAT TCGAAAATTT CAGCTTCCCC
    CCTGAGACGT GTGCTTTGGC GTCTGCAGAT CAGGATAGTT TGAGTGGGAC AGGACAGACA
    GCGGAGGAAC AGGAGCTTTC CAGTCTCACA ACACTTCACA TCGACTCTGA GACCAGCAGC
    CTTAGTCAGC ACGGCCTGTC TGCTGATACT GTCACTATTA CTGGCTCTGA GAGCGCATCT
    CCAATGCACT CTCTAGGAGG ATCTCGATCG CAGACGCCAT CTCCTGCAAC CCTGACCGCT
    GAACACGTCC AGCATAAAGA GCTGCAGCTG GATGAGAAGA TGCACCATTC TGTTCTGCAG
    ACCCCTGATG ACCTTGAAAC CAGCGAGTTT CCCACTGAGG ACTGTAGTGT AATGGCTGGT
    GGCACTCTGA CTGGCTGGCA TGCTGATGTC GCAACAGTAA TGTGGAGGAG AATGCTTGGG
    ATTCTGGGAG ATGTCAACTA TATAAAGGAC CCTGAGATCC ATGCGCAGGT TTTTGACTAC
    CTCTGCGAAC TCTGGCAGAA CCTGGCCAAG ATCAGAGACA ATCTCGGGAT CTCCCTTGAC
    AACCAGTCGT CTCCGCCACC ACCAGATCTG ATTCCACCTC TGCGGATTCT GACTCCATGG
    CTTTTCAAAG CGACTATGTT GGGCGAACGC TACAAGCAGG GAAAACTGCA CGCCTACAAG
    CTCATCTGTA AGATCATGAA GAGAAGACAG GACGTTTCCC CAAACTCTGA CTTCCTCACG
    CACTTCTACA ACATCATGCA CTGCGGCCTG CTCCACCAAG ACCAGGATAT TGTGAACACC
    ATCATTAAGC ACTGCTCTCC ACGCTTCTTC TCTATTGGAC TTCCAGGAGC CACAATGCTC
    ATCCTGGACT TCATCGTGGC AGCTAGTCGT GTCACTGCCT GCTCGTCCCT CAATGCTCCA
    CGGGTGGAGG CTCAGATCTT GTTGGGTTCC CTGGTTTGCC TCCCAAACTT GTACAATGAA
    CTTCCTGCAC TGCATCCTAC AACAGCCGAC ATCATCATGA CCAAATTCAC TGATGTCAAG
    GAGCATATAA TCAAGCACAT CCTGACCTCT GCCAGAGATG AGCCTTCTGC TCCAGCCAGG
    TGTGTGGCGC TCTGTAGTCT GGGTATTTGG CTGTGTGAAG AGCTGGTTCA TGGCACTCAG
    CATCCGCAGA TCAAAGAAGC GCTTAACGTC ATCTGTGTCA CTCTCAAGTT CTCCAATAAA
    AACGTCGCCC TGGTGGCTTC AGACATTTTG CACCTGCTGA TAAACTACGT GGATGAACTG
    GAGAAATTTC CACCCAACAC TCCAAAGAAA ATAGTGGAGG TTCTTATTGC AACCATCACT
    TACCTTCTTC CAGCCACAGA GTCCTCTCCT CATGAGCTGG ACAAGAGGCT GGTTGTTTCT
    CTACTGCTGT GCTTGCTGGA CTGGGTGATG GCTCTACCTC CGAAAACACT CCTCCAGCCG
    GTCCAAGGAG CCTCGGATAA GGAGAAAACT GACAAGTCTG TCCTCAGCTG CATCTATAAA
    GTCCTCCATG GTTGTGTCTA TGGGGCTCAG AGCTTCAGTA ACCCCAGTTA CTTCCCCATT
    CACCTGTCAG ACTTAAGCAG CCCAGACTAC GACCCCTTCT TACTACTAGA AAACCTTAAG
    GAGCCGGAGC CACTCCATTC ACCTGACTCT GAACGCTCCT CCAAACTGCA ACCAGTAACT
    GAAGTGAGGA GCCGCATTCA GCAAGGCCTT GTTTCAGTTG CGGCACGCAC TGTCATATCT
    CACCTAGTCA ACCATCTGGG TCACTATCCC ATGAGTGGTG GTCCGGCCAC TCTGACGAGC
    CAGGTGTGCG AGAACCAGGA CAACCCATAC AGTGAGAGCG CAGACCTGAC CCCTGAACTC
    TTTGACGCCC CCAATTTGCA GTTCTTCGTG CTTAATGGCA CCACCCTGCT GTCCTACTTA
    CAAATTCGGG CTGAAGATGG CCTGCCCGGT GGTGGGATGT CAGCTGGCCT CACCACCACC
    AATGCTTGTG TTCGGGTGAT TGTGAGGGAC ATTTCTGGTA AACACTCCTG GGACTCAGCT
    GTTCTGTATG GTCCACCTCA TTGCATCACT CCTAGTCAAA CTTTGCACCT TTACACGTCC
    TCTAATCCGA GAGGGGAACA TAATAGAGAT GAGGACAGTT TAGAGAGGGG CAGCCAAGAA
    GAACCAGAGG ATGGAGCTTT TCAGGAAAGC GAGGAGGACT TGGATGAGCA TGAGGAGGCG
    GAGGTGAAAG AAGACGAGGT GGAGGAGGAA GAACTTGAGG AAGAGATTGA GGAGGAAGAG
    GTTGCACTGG AACTTATGGC CCCGCAGGCC AAACGGCGGT GCAGAGAGGT CATCCCGAAC
    TGGGACTCCC TGCAGGATGG GGAAGACTCC CTAGATGAGA TGCTGCAATA TCTGGGATAC
    TCCAGCCCAG AGTGTCTGCA ACGTACTGGT GGACCTCTCA ACATACCTGC CCCACCTCCA
    AACTGTGTGT CCGAGAAACA GGAGAATGAT GTCATCAATG CCATCCTGAA GCAGTGTGCC
    GAGGAGCAGG ACTTCACAAT TCACCGTGGT AATGAGCTGA ACATGAGGGC CGTCGTTCAG
    CAGGAGCCAA GCCCACAGAG ACCACAGTCT GCCTTCTACT ACTGCAAACT GCTCCTCAAC
    ATACTAGGCA TGAACTCTTG GGAGAAGAGG AACAGTTTCC ACCTGCTGAA AAAGAATGAA
    AAACTACTGA GAGAGCTGAA GAATCTGGAC TCCAGGCAGT GTCGTGAAAC ACACAAAATC
    GCGGTGTTTT ACGTGGCAGA GGGACAAGAA GACAAGCACT CCATTCTGTC CAACACAGCC
    GGGAGTCAGG CGTATGAAGA CTTTGTATCA GGCCTGGGCT GGGAGGTGAA CCTGACGAGT
    CACTGTGGCT TTATGGGTGG CCTGCAGCGG AACAAGAGCA CGGGTTTTAC CATGCCTTAC
    TTTGCCACCT CTACAGTGGA AGTGATGTTC CACGTCTCCA CACGCATGCC TCCAGACTCA
    GACGACTCGC TCACTAAAAA GTTGAGACAC CTGGGTAATG ATGAAGTGCA CATCGTGTGG
    TCTGAGCACT CGCGGGACTA TCGGAGAGGC GTCATTCCTA CTGAGTTTGG AGACGTGCTC
    ATCGTCATCT ACCCTATGAA GAACCACATG TACAGCATCC AGATCATCAA GAAACCTGAG
    GTGCCGTTCT TTGGTCCCTT GTTTGACGGA GCCATAGTGG ATGGGACTAT ATTGCCCACA
    GTGGTGCGAG CGACAGCCAT CAACGCCAGC AGAGCTCTGA AATCCCTCAT CCCTCTATAC
    CAAAACTTCT ATGAAGAGAG GGCCAGATAT CTGGAGACGA TAGTCCAACA TCACTTGGAG
    CCCACGACAT TTGAGGACTA CGCCGCTCGT GTCTTTTGTC CTGCGCCTTT CCACTATCTT
    CCGTCTGAAG CAGGCTCATG CCTCGAGAGT CAGCTGGCAG AAAGTCCAGC AATGCAGGTC
    GACGGGAGCG ACTTAGCCTC GCCAATGTCA CCTCGTGCTA GCAAGAGCCG GATGTCCATG
    AAGCTCCGCC GCTCCTCGGG CTCCGCCAAC AAGACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322993.1
    CDS598..7056
    Translation

    Target ORF information:

    RefSeq Version XM_021467318.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTTCTCCA AAAAGCCTCA TGGAGATGTC AGGAAATCGA CACAGAAAGT GCTGGACCCC 
    AAGAAGGACG TGCTGACGAG GTTAAAGCAT CTGAGGATCG TTATCGAAAA TGCGGAGTCT
    GCTGAACTCA AGCACCTCTT TGAGCAGAAT TACTCCCACA TCTACTATGT CTTCTTTGAA
    AACTTTGTCA CCATCGAGGT TAACCTGAAA CAGAAAGGTC ACAAATCCCA ACGGGAAGAG
    CTCGATTCCA TCCTTTTTAT ATTTGAGAAA ATCCTACAGC TGTTACCAGA GCGAATACAA
    GGAAGATGGC AGTTCCACAG CATAGGTCTC ATTTTGAAGA AACTGTTACA CACTGGAAAC
    TCCCTAAAGA TCCGGAGGGA AGGGGTGCGT CTGTTCCTTC TGTGGATGCA GGCTCTGCAG
    CACAATGCTT TAAGAGAGCA GCTTTGGATC TTTGCCTGTC TGATCCCAGG ATTCCCTGCA
    CCACAGTCCG AGCTGGGCCC TCGAACCCTG GATAATTTGA TCAGCCCTCC TCTTAACCTT
    CAGGAGCCTC AAGTGACTCC AGAGGAGATC AGTCCCCTTG TCCCTCCCCA GTCAGGAGAT
    AAATCCCAGG AGGATCTCAC TGGCTACTTC CTGGAGGCTT TGCTAAAATA CATGGTAAAC
    CAGGCCAAAA GTCTGGAGTG GAAGTGCAAG GAGAACCACG AGAAGGGCTT CGCATTTCTG
    TTCGCCAACT TTAAAAAGTA TTACCTGCCC CATATCTTCC CCAACTTCTC CAAGGAGACC
    AGTTTGTACA ACCCTATTTT GGAGGTTCCA CCCATGAGAC CAAAGCCTTA CTACATGGTT
    GTAAAAAGAG ACCCAGAGAC CAATGAAGCA CTGTACTGCA CTAAAGAGAA CTTCCTCAAT
    GCCAGAGTCA TATTCATCCG CTGGCTGGTC TCCTTCTGGC TGGAGCCGCG GTCGAACACA
    GGAACACAGA TCCCCGGCAT GGAAGGAGAA AATGTGCCAA AGAATATCCA GAGAGCGGCA
    GCAGGCCTAG CAGCTCGAGG AGAAGATGGC GGCCCACGAC AGGACAGTCT GGATGGTGGT
    GGTATTGGCG AACCAGAGCA GTCGCACTCC AACACCAGCA CCCTGACGGA GAGAGAGCCC
    AGCTCCTCCA GCCTCTGCAG CATGGATGAA GAGCACCTCA CCGACATCGA GGTGGTGCGC
    CGGGTCCTGT GCTCCTCCAG AACCAACGTC AACTTCATCA CAGAGATATT TCGCCAGGCG
    TTCCTGCTGC CGATGTGTGA GGCTGCAGCC ATGCGTAAGG TGGTGCGAGT GTATCAAGAG
    TGGATTTCTC AGCAGGATAA GCCTGTGTTT ATGAGACAAC CAGAGGAAGA GCGATTCACA
    GGCCAGCTGG ACTCAACTCA CGAGCAGAGA GCTGATAAAG ACGAGGAGGA GGATGTTGGG
    CTCACGGTGT CAGTTCGAAA CCCTAATTGG AGTAGGAGTA AAAGCTCTGA TTCTGAGTAC
    AATGTTCACG CTGGAGTCCA GGCTACACTA CAGGTTTTTA TCACCAATTC CTCTAATGTC
    TTCCTTCTGG AGGCTGCCAA TGAGCTCAAA TCACTACTGG AAGAGCATGT GGACATGTGC
    AAGCGCGTGC TGAACATTTA CCGCAGTCTG GTCATGCATG AAACCATGAA CCAGAAGACC
    TGGGAGCAGA TTTTGCTAGT GTTGCTGCGA GTGACTGAGT GTGTGATGAA GAGGCCTCCC
    TCCATTATGC CTCATGGAAA GAAGAGTAGC ACTTTATCAG GCCGTTTGGC TGGAGCCATT
    TTTCAGACTC TGATTGTGGC CTGGATCAAG GGGAATCTGA ACGTGTACAT CTCTCGAGAG
    CTGTGGGATG ACCTGCTGTC TGTCCTGTCC TCTCTCACCT GCTGGGAAGA GCTGGTCACC
    GAGTGGTCCC TCACAATGGA GACACTGACC AAGGTGCTGG CACGAAACCT TTACAGCCTG
    GACCTGAACG AGCTGCCCTT GGACAAACTT AGTGAGCAGA AGCAGAAGAA GCACAAAGGC
    AAAGGTGGTA TCCATGAGGG CCAGAAAATC ACTGTGGATC GGTCGTTCTC TAAAGGCTGG
    AGTCGAGATC CTCCCGGTCA GGCGGCGGCC ATGAGACAGC GCAGCGCCAC CACCGCTGGA
    TCACCAGGCA TCGAGAAGGC CAGAAGCATC GTCCGACAGA AGACAGTCGC TCTTCGTAGT
    TGTTCTACGG GCGACAGTCT GCTGTCCTCA GCCTTTATCC GCAGTGCTAA AAGCGCACCT
    GTCCTGATCC ACCCCGTACA CCCTCTCCTG CCTGACTCTG TCCTCACTCC TCTAGCTGAC
    GAGCTGTCAG ACCTGGAGGA GCCTCCTATG ATCCAGCGTG GGCCACGGAA CCGCCACTGC
    TCTCAGAGCG AGGAGACGCC GACGTCTGAA GTTTTTTCGG CCTCCGGTGA ACTGGAGCAG
    CCTCCGCTTC CCCGCAGTAG CAGTGCCTCC GACATCATGG AGCCCTTCAT CGCAGAACGG
    GCCAAAGCCA ATAAAGACGA GCTGACGCCG AAGATGCGGC CCATGTCTAA CGATGCTGGC
    AGCAGCAACC CCAATGTCAG TGACCTCATG GACGAGTTCA TCCAGGAGAG ACTGAGAGCC
    AGAGGCAATT CTGGCATGAC GAGGCGCGGC AGTAGCCCAG GAAGTCTGGA GATCCCTAAA
    GATCTGCCAG AGCTGCTGAA CCGTCAGAAC GCCACACGTC CCGCAGACGA CCCCGGTGTG
    CCCTCTGAGT GGACCTCGCC TGCCAGTGCC AGTGGAAGTG ACCTCATAAG CTCTGACAGC
    CAATCGGATT CCTTTAACGC TTTTCAATAT GACAGCAAAT TCGAAAATTT CAGCTTCCCC
    CCTGAGACGT GTGCTTTGGC GTCTGCAGAT CAGGATAGTT TGAGTGGGAC AGGACAGACA
    GCGGAGGAAC AGGAGCTTTC CAGTCTCACA ACACTTCACA TCGACTCTGA GACCAGCAGC
    CTTAGTCAGC ACGGCCTGTC TGCTGATACT GTCACTATTA CTGGCTCTGA GAGCGCATCT
    CCAATGCACT CTCTAGGAGG ATCTCGATCG CAGACGCCAT CTCCTGCAAC CCTGACCGCT
    GAACACGTCC AGCATAAAGA GCTGCAGCTG GATGAGAAGA TGCACCATTC TGTTCTGCAG
    ACCCCTGATG ACCTTGAAAC CAGCGAGTTT CCCACTGAGG ACTGTAGTGT AATGGCTGGT
    GGCACTCTGA CTGGCTGGCA TGCTGATGTC GCAACAGTAA TGTGGAGGAG AATGCTTGGG
    ATTCTGGGAG ATGTCAACTA TATAAAGGAC CCTGAGATCC ATGCGCAGGT TTTTGACTAC
    CTCTGCGAAC TCTGGCAGAA CCTGGCCAAG ATCAGAGACA ATCTCGGGAT CTCCCTTGAC
    AACCAGTCGT CTCCGCCACC ACCAGATCTG ATTCCACCTC TGCGGATTCT GACTCCATGG
    CTTTTCAAAG CGACTATGTT GGGCGAACGC TACAAGCAGG GAAAACTGCA CGCCTACAAG
    CTCATCTGTA AGATCATGAA GAGAAGACAG GACGTTTCCC CAAACTCTGA CTTCCTCACG
    CACTTCTACA ACATCATGCA CTGCGGCCTG CTCCACCAAG ACCAGGATAT TGTGAACACC
    ATCATTAAGC ACTGCTCTCC ACGCTTCTTC TCTATTGGAC TTCCAGGAGC CACAATGCTC
    ATCCTGGACT TCATCGTGGC AGCTAGTCGT GTCACTGCCT GCTCGTCCCT CAATGCTCCA
    CGGGTGGAGG CTCAGATCTT GTTGGGTTCC CTGGTTTGCC TCCCAAACTT GTACAATGAA
    CTTCCTGCAC TGCATCCTAC AACAGCCGAC ATCATCATGA CCAAATTCAC TGATGTCAAG
    GAGCATATAA TCAAGCACAT CCTGACCTCT GCCAGAGATG AGCCTTCTGC TCCAGCCAGG
    TGTGTGGCGC TCTGTAGTCT GGGTATTTGG CTGTGTGAAG AGCTGGTTCA TGGCACTCAG
    CATCCGCAGA TCAAAGAAGC GCTTAACGTC ATCTGTGTCA CTCTCAAGTT CTCCAATAAA
    AACGTCGCCC TGGTGGCTTC AGACATTTTG CACCTGCTGA TAAACTACGT GGATGAACTG
    GAGAAATTTC CACCCAACAC TCCAAAGAAA ATAGTGGAGG TTCTTATTGC AACCATCACT
    TACCTTCTTC CAGCCACAGA GTCCTCTCCT CATGAGCTGG ACAAGAGGCT GGTTGTTTCT
    CTACTGCTGT GCTTGCTGGA CTGGGTGATG GCTCTACCTC CGAAAACACT CCTCCAGCCG
    GTCCAAGGAG CCTCGGATAA GGAGAAAACT GACAAGTCTG TCCTCAGCTG CATCTATAAA
    GTCCTCCATG GTTGTGTCTA TGGGGCTCAG AGCTTCAGTA ACCCCAGTTA CTTCCCCATT
    CACCTGTCAG ACTTAAGCAG CCCAGACTAC GACCCCTTCT TACTACTAGA AAACCTTAAG
    GAGCCGGAGC CACTCCATTC ACCTGACTCT GAACGCTCCT CCAAACTGCA ACCAGTAACT
    GAAGTGAGGA GCCGCATTCA GCAAGGCCTT GTTTCAGTTG CGGCACGCAC TGTCATATCT
    CACCTAGTCA ACCATCTGGG TCACTATCCC ATGAGTGGTG GTCCGGCCAC TCTGACGAGC
    CAGGTGTGCG AGAACCAGGA CAACCCATAC AGTGAGAGCG CAGACCTGAC CCCTGAACTC
    TTTGACGCCC CCAATTTGCA GTTCTTCGTG CTTAATGGCA CCACCCTGCT GTCCTACTTA
    CAAATTCGGG CTGAAGATGG CCTGCCCGGT GGTGGGATGT CAGCTGGCCT CACCACCACC
    AATGCTTGTG TTCGGGTGAT TGTGAGGGAC ATTTCTGGTA AACACTCCTG GGACTCAGCT
    GTTCTGTATG GTCCACCTCA TTGCATCACT CCTAGTCAAA CTTTGCACCT TTACACGTCC
    TCTAATCCGA GAGGGGAACA TAATAGAGAT GAGGACAGTT TAGAGAGGGG CAGCCAAGAA
    GAACCAGAGG ATGGAGCTTT TCAGGAAAGC GAGGAGGACT TGGATGAGCA TGAGGAGGCG
    GAGGTGAAAG AAGACGAGGT GGAGGAGGAA GAACTTGAGG AAGAGATTGA GGAGGAAGAG
    GTTGCACTGG AACTTATGGC CCCGCAGGCC AAACGGCGGT GCAGAGAGGT CATCCCGAAC
    TGGGACTCCC TGCAGGATGG GGAAGACTCC CTAGATGAGA TGCTGCAATA TCTGGGATAC
    TCCAGCCCAG AGTGTCTGCA ACGTACTGGT GGACCTCTCA ACATACCTGC CCCACCTCCA
    AACTGTGTGT CCGAGAAACA GGAGAATGAT GTCATCAATG CCATCCTGAA GCAGTGTGCC
    GAGGAGCAGG ACTTCACAAT TCACCGTGGT AATGAGCTGA ACATGAGGGC CGTCGTTCAG
    CAGGAGCCAA GCCCACAGAG ACCACAGTCT GCCTTCTACT ACTGCAAACT GCTCCTCAAC
    ATACTAGGCA TGAACTCTTG GGAGAAGAGG AACAGTTTCC ACCTGCTGAA AAAGAATGAA
    AAACTACTGA GAGAGCTGAA GAATCTGGAC TCCAGGCAGT GTCGTGAAAC ACACAAAATC
    GCGGTGTTTT ACGTGGCAGA GGGACAAGAA GACAAGCACT CCATTCTGTC CAACACAGCC
    GGGAGTCAGG CGTATGAAGA CTTTGTATCA GGCCTGGGCT GGGAGGTGAA CCTGACGAGT
    CACTGTGGCT TTATGGGTGG CCTGCAGCGG AACAAGAGCA CGGGTTTTAC CATGCCTTAC
    TTTGCCACCT CTACAGTGGA AGTGATGTTC CACGTCTCCA CACGCATGCC TCCAGACTCA
    GACGACTCGC TCACTAAAAA GTTGAGACAC CTGGGTAATG ATGAAGTGCA CATCGTGTGG
    TCTGAGCACT CGCGGGACTA TCGGAGAGGC GTCATTCCTA CTGAGTTTGG AGACGTGCTC
    ATCGTCATCT ACCCTATGAA GAACCACATG TACAGCATCC AGATCATCAA GAAACCTGAG
    GTGCCGTTCT TTGGTCCCTT GTTTGACGGA GCCATAGTGG ATGGGACTAT ATTGCCCACA
    GTGGTGCGAG CGACAGCCAT CAACGCCAGC AGAGCTCTGA AATCCCTCAT CCCTCTATAC
    CAAAACTTCT ATGAAGAGAG GGCCAGATAT CTGGAGACGA TAGTCCAACA TCACTTGGAG
    CCCACGACAT TTGAGGACTA CGCCGCTCGT GTCTTTTGTC CTGCGCCTTT CCACTATCTT
    CCGTCTGAAG CAGGCTCATG CCTCGAGAGT CAGCTGGCAG AAAGTCCAGC AATGCAGGTC
    GACGGGAGCG ACTTAGCCTC GCCAATGTCA CCTCGTGCTA GCAAGAGCCG GATGTCCATG
    AAGCTCCGCC GCTCCTCGGG CTCCGCCAAC AAGACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.