RALGAPA1 cDNA ORF clone, Homo sapiens(human)

The following RALGAPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RALGAPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu47637 XM_005267498.4
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu84854 XM_017021147.1
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu78242 XM_024449523.1
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu84853 XM_017021146.1
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu47636 XM_006720104.4
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu78241 XM_011536616.3
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu102383 NM_001346248.2
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu102383 NM_001330075.3
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu84852 XM_017021143.2
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu02421 NM_194301.4
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu05247 NM_001283043.3
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu06762 NM_014990.3
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu06762 NM_001346243.2
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu10997 NM_001346245.2
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu10997 NM_001283044.3
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu47632 NM_001346249.2
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu47633 NM_001346247.2
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu47635 NM_001346246.2
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu47629 XM_006720098.3
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu47630 XM_006720099.3
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu47631 XM_006720100.4
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu47632 XM_011536615.3
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu47632 XM_006720101.3
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu47634 XM_005267492.4
Latest version!
Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OHu47633 NM_001346247.1 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu06762 NM_001346243.1 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu47637 XM_005267498.3 Homo sapiens Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu47630 XM_006720099.2 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu10997 NM_001283044.2 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu47632 NM_001346249.1 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu06762 NM_014990.2 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu47632 XM_006720101.2 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu84852 XM_017021143.1 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu47629 XM_006720098.2 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu47632 XM_011536615.2 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu47634 XM_005267492.3 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu47635 NM_001346246.1 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu47631 XM_006720100.3 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu10997 NM_001346245.1 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu05247 NM_001283043.2 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu47636 XM_006720104.3 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu102383 NM_001346248.1 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu78241 XM_011536616.2 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu02421 NM_194301.3 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu102383 NM_001330075.2 Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu47637
    Clone ID Related Accession (Same CDS sequence) XM_005267498.4 , XM_005267498.3
    Accession Version XM_005267498.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005267498.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGAGCA GCCATTGCCT CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG 
    TTGAACGAGC CAAAGAGGCA GAACAGAATG CCACCAGAGG CTCCACTGAG GGATCTGTAC
    AGTCATGTAA TGGGCTATTT TGGAAGGAAA GCTGCAGTCA ATAAAGAGGA CATGAGCCAA
    AAACTGCCTC CTCTTAATAG TGATATTGGC GGCAGCAGTG CTAATGTTCC TGATCTGATG
    GATGAGTTTA TAGCAGAACG ACTTCGAAGT GGTAATGCCT CGACTATGAC AAGAAGAGGA
    AGTAGTCCAG GCAGCCTGGA AATTCCCAAA GACCTCCCTG ATATTCTAAA CAAGCAGAAC
    CAGATGCGCC CTATTGATGA CCCAGGTGTG CCCTCAGAAT GGACTTCTCC TGCCAGTGCA
    GGGAGCAGTG ATCTTATCAG CTCAGATAGT CATTCGGATT CTTTCAGCGC TTTCCAATAT
    GATGGCCGAA AATTTGACAA TTTTGGCTTT GGAACCGACA CTGGGGTTAC GTCCTCTGCT
    GATGTGGATT CAGGTTCTGG CCATCATCAG AGTGCTGAAG AGCAGGAAGT GGCTAGTCTA
    ACTACTCTTC ATATAGATTC TGAAACAAGC AGTCTTAATC AGCAAGCTTT CTCTGCTGAA
    GTTGCAACTA TTACTGGTTC AGAAAGTGCT TCCCCAGTCC ACTCACCTCT GGGCTCCAGG
    TCACAGACTC CTTCCCCTTC TACATTGAAT ATAGATCACA TGGAACAGAA GGATCTGCAG
    CTCGACGAGA AGCTCCACCA CTCTGTTCTT CAGACACCAG ATGATCTAGA AATTAGTGAA
    TTTCCATCAG AATGTTGTAG TGTGATGGCA GGAGGTACTC TGACTGGATG GCATGCTGAT
    GTTGCTACTG TAATGTGGCG AAGAATGCTA GGCATTTTGG GAGATGTAAA TTCAATCATG
    GATCCTGAAA TACATGCTCA AGTTTTTGAT TACCTCTGTG AACTTTGGCA GAATCTAGCT
    AAGATTAGAG ATAACCTTGG CATTTCAACT GATAACCTGA CCTCCCCTTC TCCACCAGTT
    TTAATTCCTC CACTGAGAAT TCTTACACCT TGGCTTTTTA AGGCAACCAT GTTGACTGAT
    AAATATAAAC AAGGTAAATT ACATGCATAT AAACTTATTT GTAATACAAT GAAAAGAAGA
    CAAGATGTTT CTCCAAATAG AGATTTTCTA ACACATTTCT ACAATATAAT GCATTGTGGA
    TTACTTCATA TTGACCAGGA TATTGTCAAT ACAATCATCA AACACTGCTC ACCTCAATTT
    TTTTCACTTG GTTTGCCTGG TGCCACAATG CTTATTATGG ATTTTATTGT AGCAGCTGGT
    AGAGTGGCTT CTTCAGCTTT TCTCAATGCA CCAAGAGTAG AAGCACAAGT TCTTCTGGGA
    TCTTTGGTTT GCTTTCCCAA CTTATATTGT GAACTGCCTT CTCTTCATCC CAACATTCCT
    GATGTTGCTG TGTCTCAGTT TACAGATGTT AAGGAACTTA TAATCAAAAC TGTATTAAGC
    TCGGCAAGAG ATGAGCCCTC TGGTCCTGCA CGATGTGTAG CACTTTGTAG TTTAGGTATT
    TGGATTTGTG AAGAACTAGT CCATGAGTCT CATCATCCTC AAATTAAGGA AGCTCTGAAT
    GTGATTTGTG TTTCCTTAAA GTTTACTAAT AAAACAGTAG CCCACGTAGC TTGTAACATG
    CTTCACATGC TGGTTCATTA TGTACCTAGA CTTCAGATTT ACCAGCCTGA TTCTCCCTTG
    AAAATTATTC AAATCCTAAT AGCTACCATC ACCCATCTTT TACCAAGTAC AGAGGCTTCA
    TCTTATGAAA TGGACAAGAG GTTGGTAGTA TCTTTACTTC TCTGCCTTCT GGACTGGATC
    ATGGCCTTAC CTCTAAAGAC ACTGCTCCAA CCATTTCATG CTACGGGAGC AGAAAGCGAT
    AAAACAGAAA AATCTGTTCT CAATTGCATT TATAAGGTTT TACATGGGTG TGTTTATGGA
    GCTCAGTGTT TTAGCAATCC AAGGTATTTT CCCATGAGCC TCTCTGATTT GGCATCTGTA
    GATTATGATC CTTTTATGCA TTTGGAAAGT CTGAAAGAGC CTGAGCCTCT GCACTCTCCT
    GACTCAGAAC GATCTTCTAA ACTCCAGCCA GTAACAGAAG TGAAAACTCA AATGCAGCAT
    GGATTAATCT CTATAGCAGC CCGCACTGTT ATTACACATC TGGTAAATCA CCTGGGCCAT
    TATCCAATGA GCGGTGGTCC TGCTATGCTA ACAAGTCAGG TGTGTGAAAA TCACGACAAT
    CATTACAGTG AAAGTACTGA ACTTTCTCCT GAACTCTTTG AGAGTCCAAA TATCCAGTTC
    TTTGTGTTAA ATAATACAAC CTTAGTGTCC TGTATCCAGA TCAGATCAGA AGAGAATATG
    CCTGGAGGAG GTTTATCTGC TGGCCTTGCA TCAGCCAATT CAAATGTCAG AATCATAGTA
    CGTGATCTCT CTGGAAAATA TTCATGGGAT TCTGCTATAC TGTATGGCCC ACCTCCTGTA
    AGTGGCTTGT CAGAACCTAC ATCTTTCATG CTTTCATTGT CTCACCAAGA GAAGCCAGAA
    GAGCCTCCGA CATCTAATGA ATGCTTAGAA GATATAACCG TAAAAGATGG ACTTTCTCTC
    CAGTTTAAAA GATTTAGAGA AACTGTACCA ACTTGGGATA CAATAAGAGA TGAAGAAGAT
    GTTCTTGATG AGCTCTTGCA GTATTTGGGT GTTACTAGTC CTGAATGCTT ACAGAGAACT
    GGAATCTCAC TTAATATTCC TGCTCCACAA CCTGTGTGCA TTTCTGAAAA ACAAGAAAAT
    GATGTTATTA ATGCTATCCT TAAGCAACAT ACAGAAGAAA AAGAATTTGT TGAGAAGCAC
    TTTAATGACT TAAACATGAA AGCTGTGGAA CAAGATGAAC CAATACCTCA AAAACCTCAG
    TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA
    CGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAGCTAC TTAGAGAACT TAGGAACTTG
    GATTCAAGGC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA
    GAAGACAAAC ACTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA
    GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTTATGGG AGGACTACAA
    AAAAACAAAA GCACTGGATT GACCACTCCA TATTTTGCTA CCTCTACAGT AGAGGTAATA
    TTTCACGTGT CAACAAGAAT GCCTTCTGAT TCTGATGATT CTTTGACCAA AAAATTGAGA
    CATTTGGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA
    GGAATTATTC CCACAGAATT TGGTGATGTC CTTATTGTAA TATATCCAAT GAAAAATCAC
    ATGTTCAGTA TTCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC CCTTTTTGAT
    GGTGCTATTG TGAATGGAAA GGTTCTACCC ATTATGGTTA GAGCAACAGC TATAAATGCA
    AGCCGTGCTC TGAAATCTCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA
    TACCTGCAAA CAATTGTCCA GCACCACTTA GAACCAACAA CATTTGAAGA TTTTGCAGCA
    CAGGTTTTTT CTCCAGCTCC CTACCACCAT TTACCATCTG ATGCCGTTGG CTCCTACCCA
    GAGATTCTAC CCAGTGAAAC TCCCACAGCA ACGCAGGTAG ATGGGGCTGA CCTGGCCTCT
    CCAATGTCTC CTCGAACTAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG TTCCTCTGGC
    TCAGCCAATA AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005267555.1
    CDS498..4475
    Translation

    Target ORF information:

    RefSeq Version XM_005267498.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005267498.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGAGCA GCCATTGCCT CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG 
    TTGAACGAGC CAAAGAGGCA GAACAGAATG CCACCAGAGG CTCCACTGAG GGATCTGTAC
    AGTCATGTAA TGGGCTATTT TGGAAGGAAA GCTGCAGTCA ATAAAGAGGA CATGAGCCAA
    AAACTGCCTC CTCTTAATAG TGATATTGGC GGCAGCAGTG CTAATGTTCC TGATCTGATG
    GATGAGTTTA TAGCAGAACG ACTTCGAAGT GGTAATGCCT CGACTATGAC AAGAAGAGGA
    AGTAGTCCAG GCAGCCTGGA AATTCCCAAA GACCTCCCTG ATATTCTAAA CAAGCAGAAC
    CAGATGCGCC CTATTGATGA CCCAGGTGTG CCCTCAGAAT GGACTTCTCC TGCCAGTGCA
    GGGAGCAGTG ATCTTATCAG CTCAGATAGT CATTCGGATT CTTTCAGCGC TTTCCAATAT
    GATGGCCGAA AATTTGACAA TTTTGGCTTT GGAACCGACA CTGGGGTTAC GTCCTCTGCT
    GATGTGGATT CAGGTTCTGG CCATCATCAG AGTGCTGAAG AGCAGGAAGT GGCTAGTCTA
    ACTACTCTTC ATATAGATTC TGAAACAAGC AGTCTTAATC AGCAAGCTTT CTCTGCTGAA
    GTTGCAACTA TTACTGGTTC AGAAAGTGCT TCCCCAGTCC ACTCACCTCT GGGCTCCAGG
    TCACAGACTC CTTCCCCTTC TACATTGAAT ATAGATCACA TGGAACAGAA GGATCTGCAG
    CTCGACGAGA AGCTCCACCA CTCTGTTCTT CAGACACCAG ATGATCTAGA AATTAGTGAA
    TTTCCATCAG AATGTTGTAG TGTGATGGCA GGAGGTACTC TGACTGGATG GCATGCTGAT
    GTTGCTACTG TAATGTGGCG AAGAATGCTA GGCATTTTGG GAGATGTAAA TTCAATCATG
    GATCCTGAAA TACATGCTCA AGTTTTTGAT TACCTCTGTG AACTTTGGCA GAATCTAGCT
    AAGATTAGAG ATAACCTTGG CATTTCAACT GATAACCTGA CCTCCCCTTC TCCACCAGTT
    TTAATTCCTC CACTGAGAAT TCTTACACCT TGGCTTTTTA AGGCAACCAT GTTGACTGAT
    AAATATAAAC AAGGTAAATT ACATGCATAT AAACTTATTT GTAATACAAT GAAAAGAAGA
    CAAGATGTTT CTCCAAATAG AGATTTTCTA ACACATTTCT ACAATATAAT GCATTGTGGA
    TTACTTCATA TTGACCAGGA TATTGTCAAT ACAATCATCA AACACTGCTC ACCTCAATTT
    TTTTCACTTG GTTTGCCTGG TGCCACAATG CTTATTATGG ATTTTATTGT AGCAGCTGGT
    AGAGTGGCTT CTTCAGCTTT TCTCAATGCA CCAAGAGTAG AAGCACAAGT TCTTCTGGGA
    TCTTTGGTTT GCTTTCCCAA CTTATATTGT GAACTGCCTT CTCTTCATCC CAACATTCCT
    GATGTTGCTG TGTCTCAGTT TACAGATGTT AAGGAACTTA TAATCAAAAC TGTATTAAGC
    TCGGCAAGAG ATGAGCCCTC TGGTCCTGCA CGATGTGTAG CACTTTGTAG TTTAGGTATT
    TGGATTTGTG AAGAACTAGT CCATGAGTCT CATCATCCTC AAATTAAGGA AGCTCTGAAT
    GTGATTTGTG TTTCCTTAAA GTTTACTAAT AAAACAGTAG CCCACGTAGC TTGTAACATG
    CTTCACATGC TGGTTCATTA TGTACCTAGA CTTCAGATTT ACCAGCCTGA TTCTCCCTTG
    AAAATTATTC AAATCCTAAT AGCTACCATC ACCCATCTTT TACCAAGTAC AGAGGCTTCA
    TCTTATGAAA TGGACAAGAG GTTGGTAGTA TCTTTACTTC TCTGCCTTCT GGACTGGATC
    ATGGCCTTAC CTCTAAAGAC ACTGCTCCAA CCATTTCATG CTACGGGAGC AGAAAGCGAT
    AAAACAGAAA AATCTGTTCT CAATTGCATT TATAAGGTTT TACATGGGTG TGTTTATGGA
    GCTCAGTGTT TTAGCAATCC AAGGTATTTT CCCATGAGCC TCTCTGATTT GGCATCTGTA
    GATTATGATC CTTTTATGCA TTTGGAAAGT CTGAAAGAGC CTGAGCCTCT GCACTCTCCT
    GACTCAGAAC GATCTTCTAA ACTCCAGCCA GTAACAGAAG TGAAAACTCA AATGCAGCAT
    GGATTAATCT CTATAGCAGC CCGCACTGTT ATTACACATC TGGTAAATCA CCTGGGCCAT
    TATCCAATGA GCGGTGGTCC TGCTATGCTA ACAAGTCAGG TGTGTGAAAA TCACGACAAT
    CATTACAGTG AAAGTACTGA ACTTTCTCCT GAACTCTTTG AGAGTCCAAA TATCCAGTTC
    TTTGTGTTAA ATAATACAAC CTTAGTGTCC TGTATCCAGA TCAGATCAGA AGAGAATATG
    CCTGGAGGAG GTTTATCTGC TGGCCTTGCA TCAGCCAATT CAAATGTCAG AATCATAGTA
    CGTGATCTCT CTGGAAAATA TTCATGGGAT TCTGCTATAC TGTATGGCCC ACCTCCTGTA
    AGTGGCTTGT CAGAACCTAC ATCTTTCATG CTTTCATTGT CTCACCAAGA GAAGCCAGAA
    GAGCCTCCGA CATCTAATGA ATGCTTAGAA GATATAACCG TAAAAGATGG ACTTTCTCTC
    CAGTTTAAAA GATTTAGAGA AACTGTACCA ACTTGGGATA CAATAAGAGA TGAAGAAGAT
    GTTCTTGATG AGCTCTTGCA GTATTTGGGT GTTACTAGTC CTGAATGCTT ACAGAGAACT
    GGAATCTCAC TTAATATTCC TGCTCCACAA CCTGTGTGCA TTTCTGAAAA ACAAGAAAAT
    GATGTTATTA ATGCTATCCT TAAGCAACAT ACAGAAGAAA AAGAATTTGT TGAGAAGCAC
    TTTAATGACT TAAACATGAA AGCTGTGGAA CAAGATGAAC CAATACCTCA AAAACCTCAG
    TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA
    CGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAGCTAC TTAGAGAACT TAGGAACTTG
    GATTCAAGGC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA
    GAAGACAAAC ACTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA
    GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTTATGGG AGGACTACAA
    AAAAACAAAA GCACTGGATT GACCACTCCA TATTTTGCTA CCTCTACAGT AGAGGTAATA
    TTTCACGTGT CAACAAGAAT GCCTTCTGAT TCTGATGATT CTTTGACCAA AAAATTGAGA
    CATTTGGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA
    GGAATTATTC CCACAGAATT TGGTGATGTC CTTATTGTAA TATATCCAAT GAAAAATCAC
    ATGTTCAGTA TTCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC CCTTTTTGAT
    GGTGCTATTG TGAATGGAAA GGTTCTACCC ATTATGGTTA GAGCAACAGC TATAAATGCA
    AGCCGTGCTC TGAAATCTCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA
    TACCTGCAAA CAATTGTCCA GCACCACTTA GAACCAACAA CATTTGAAGA TTTTGCAGCA
    CAGGTTTTTT CTCCAGCTCC CTACCACCAT TTACCATCTG ATGCCGTTGG CTCCTACCCA
    GAGATTCTAC CCAGTGAAAC TCCCACAGCA ACGCAGGTAG ATGGGGCTGA CCTGGCCTCT
    CCAATGTCTC CTCGAACTAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG TTCCTCTGGC
    TCAGCCAATA AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47637
    Clone ID Related Accession (Same CDS sequence) XM_005267498.4 , XM_005267498.3
    Accession Version XM_005267498.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X12
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGAGCA GCCATTGCCT CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG 
    TTGAACGAGC CAAAGAGGCA GAACAGAATG CCACCAGAGG CTCCACTGAG GGATCTGTAC
    AGTCATGTAA TGGGCTATTT TGGAAGGAAA GCTGCAGTCA ATAAAGAGGA CATGAGCCAA
    AAACTGCCTC CTCTTAATAG TGATATTGGC GGCAGCAGTG CTAATGTTCC TGATCTGATG
    GATGAGTTTA TAGCAGAACG ACTTCGAAGT GGTAATGCCT CGACTATGAC AAGAAGAGGA
    AGTAGTCCAG GCAGCCTGGA AATTCCCAAA GACCTCCCTG ATATTCTAAA CAAGCAGAAC
    CAGATGCGCC CTATTGATGA CCCAGGTGTG CCCTCAGAAT GGACTTCTCC TGCCAGTGCA
    GGGAGCAGTG ATCTTATCAG CTCAGATAGT CATTCGGATT CTTTCAGCGC TTTCCAATAT
    GATGGCCGAA AATTTGACAA TTTTGGCTTT GGAACCGACA CTGGGGTTAC GTCCTCTGCT
    GATGTGGATT CAGGTTCTGG CCATCATCAG AGTGCTGAAG AGCAGGAAGT GGCTAGTCTA
    ACTACTCTTC ATATAGATTC TGAAACAAGC AGTCTTAATC AGCAAGCTTT CTCTGCTGAA
    GTTGCAACTA TTACTGGTTC AGAAAGTGCT TCCCCAGTCC ACTCACCTCT GGGCTCCAGG
    TCACAGACTC CTTCCCCTTC TACATTGAAT ATAGATCACA TGGAACAGAA GGATCTGCAG
    CTCGACGAGA AGCTCCACCA CTCTGTTCTT CAGACACCAG ATGATCTAGA AATTAGTGAA
    TTTCCATCAG AATGTTGTAG TGTGATGGCA GGAGGTACTC TGACTGGATG GCATGCTGAT
    GTTGCTACTG TAATGTGGCG AAGAATGCTA GGCATTTTGG GAGATGTAAA TTCAATCATG
    GATCCTGAAA TACATGCTCA AGTTTTTGAT TACCTCTGTG AACTTTGGCA GAATCTAGCT
    AAGATTAGAG ATAACCTTGG CATTTCAACT GATAACCTGA CCTCCCCTTC TCCACCAGTT
    TTAATTCCTC CACTGAGAAT TCTTACACCT TGGCTTTTTA AGGCAACCAT GTTGACTGAT
    AAATATAAAC AAGGTAAATT ACATGCATAT AAACTTATTT GTAATACAAT GAAAAGAAGA
    CAAGATGTTT CTCCAAATAG AGATTTTCTA ACACATTTCT ACAATATAAT GCATTGTGGA
    TTACTTCATA TTGACCAGGA TATTGTCAAT ACAATCATCA AACACTGCTC ACCTCAATTT
    TTTTCACTTG GTTTGCCTGG TGCCACAATG CTTATTATGG ATTTTATTGT AGCAGCTGGT
    AGAGTGGCTT CTTCAGCTTT TCTCAATGCA CCAAGAGTAG AAGCACAAGT TCTTCTGGGA
    TCTTTGGTTT GCTTTCCCAA CTTATATTGT GAACTGCCTT CTCTTCATCC CAACATTCCT
    GATGTTGCTG TGTCTCAGTT TACAGATGTT AAGGAACTTA TAATCAAAAC TGTATTAAGC
    TCGGCAAGAG ATGAGCCCTC TGGTCCTGCA CGATGTGTAG CACTTTGTAG TTTAGGTATT
    TGGATTTGTG AAGAACTAGT CCATGAGTCT CATCATCCTC AAATTAAGGA AGCTCTGAAT
    GTGATTTGTG TTTCCTTAAA GTTTACTAAT AAAACAGTAG CCCACGTAGC TTGTAACATG
    CTTCACATGC TGGTTCATTA TGTACCTAGA CTTCAGATTT ACCAGCCTGA TTCTCCCTTG
    AAAATTATTC AAATCCTAAT AGCTACCATC ACCCATCTTT TACCAAGTAC AGAGGCTTCA
    TCTTATGAAA TGGACAAGAG GTTGGTAGTA TCTTTACTTC TCTGCCTTCT GGACTGGATC
    ATGGCCTTAC CTCTAAAGAC ACTGCTCCAA CCATTTCATG CTACGGGAGC AGAAAGCGAT
    AAAACAGAAA AATCTGTTCT CAATTGCATT TATAAGGTTT TACATGGGTG TGTTTATGGA
    GCTCAGTGTT TTAGCAATCC AAGGTATTTT CCCATGAGCC TCTCTGATTT GGCATCTGTA
    GATTATGATC CTTTTATGCA TTTGGAAAGT CTGAAAGAGC CTGAGCCTCT GCACTCTCCT
    GACTCAGAAC GATCTTCTAA ACTCCAGCCA GTAACAGAAG TGAAAACTCA AATGCAGCAT
    GGATTAATCT CTATAGCAGC CCGCACTGTT ATTACACATC TGGTAAATCA CCTGGGCCAT
    TATCCAATGA GCGGTGGTCC TGCTATGCTA ACAAGTCAGG TGTGTGAAAA TCACGACAAT
    CATTACAGTG AAAGTACTGA ACTTTCTCCT GAACTCTTTG AGAGTCCAAA TATCCAGTTC
    TTTGTGTTAA ATAATACAAC CTTAGTGTCC TGTATCCAGA TCAGATCAGA AGAGAATATG
    CCTGGAGGAG GTTTATCTGC TGGCCTTGCA TCAGCCAATT CAAATGTCAG AATCATAGTA
    CGTGATCTCT CTGGAAAATA TTCATGGGAT TCTGCTATAC TGTATGGCCC ACCTCCTGTA
    AGTGGCTTGT CAGAACCTAC ATCTTTCATG CTTTCATTGT CTCACCAAGA GAAGCCAGAA
    GAGCCTCCGA CATCTAATGA ATGCTTAGAA GATATAACCG TAAAAGATGG ACTTTCTCTC
    CAGTTTAAAA GATTTAGAGA AACTGTACCA ACTTGGGATA CAATAAGAGA TGAAGAAGAT
    GTTCTTGATG AGCTCTTGCA GTATTTGGGT GTTACTAGTC CTGAATGCTT ACAGAGAACT
    GGAATCTCAC TTAATATTCC TGCTCCACAA CCTGTGTGCA TTTCTGAAAA ACAAGAAAAT
    GATGTTATTA ATGCTATCCT TAAGCAACAT ACAGAAGAAA AAGAATTTGT TGAGAAGCAC
    TTTAATGACT TAAACATGAA AGCTGTGGAA CAAGATGAAC CAATACCTCA AAAACCTCAG
    TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA
    CGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAGCTAC TTAGAGAACT TAGGAACTTG
    GATTCAAGGC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA
    GAAGACAAAC ACTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA
    GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTTATGGG AGGACTACAA
    AAAAACAAAA GCACTGGATT GACCACTCCA TATTTTGCTA CCTCTACAGT AGAGGTAATA
    TTTCACGTGT CAACAAGAAT GCCTTCTGAT TCTGATGATT CTTTGACCAA AAAATTGAGA
    CATTTGGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA
    GGAATTATTC CCACAGAATT TGGTGATGTC CTTATTGTAA TATATCCAAT GAAAAATCAC
    ATGTTCAGTA TTCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC CCTTTTTGAT
    GGTGCTATTG TGAATGGAAA GGTTCTACCC ATTATGGTTA GAGCAACAGC TATAAATGCA
    AGCCGTGCTC TGAAATCTCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA
    TACCTGCAAA CAATTGTCCA GCACCACTTA GAACCAACAA CATTTGAAGA TTTTGCAGCA
    CAGGTTTTTT CTCCAGCTCC CTACCACCAT TTACCATCTG ATGCCGTTGG CTCCTACCCA
    GAGATTCTAC CCAGTGAAAC TCCCACAGCA ACGCAGGTAG ATGGGGCTGA CCTGGCCTCT
    CCAATGTCTC CTCGAACTAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG TTCCTCTGGC
    TCAGCCAATA AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005267555.1
    Translation

    Target ORF information:

    RefSeq Version XM_005267498.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005267498.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGAGCA GCCATTGCCT CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG 
    TTGAACGAGC CAAAGAGGCA GAACAGAATG CCACCAGAGG CTCCACTGAG GGATCTGTAC
    AGTCATGTAA TGGGCTATTT TGGAAGGAAA GCTGCAGTCA ATAAAGAGGA CATGAGCCAA
    AAACTGCCTC CTCTTAATAG TGATATTGGC GGCAGCAGTG CTAATGTTCC TGATCTGATG
    GATGAGTTTA TAGCAGAACG ACTTCGAAGT GGTAATGCCT CGACTATGAC AAGAAGAGGA
    AGTAGTCCAG GCAGCCTGGA AATTCCCAAA GACCTCCCTG ATATTCTAAA CAAGCAGAAC
    CAGATGCGCC CTATTGATGA CCCAGGTGTG CCCTCAGAAT GGACTTCTCC TGCCAGTGCA
    GGGAGCAGTG ATCTTATCAG CTCAGATAGT CATTCGGATT CTTTCAGCGC TTTCCAATAT
    GATGGCCGAA AATTTGACAA TTTTGGCTTT GGAACCGACA CTGGGGTTAC GTCCTCTGCT
    GATGTGGATT CAGGTTCTGG CCATCATCAG AGTGCTGAAG AGCAGGAAGT GGCTAGTCTA
    ACTACTCTTC ATATAGATTC TGAAACAAGC AGTCTTAATC AGCAAGCTTT CTCTGCTGAA
    GTTGCAACTA TTACTGGTTC AGAAAGTGCT TCCCCAGTCC ACTCACCTCT GGGCTCCAGG
    TCACAGACTC CTTCCCCTTC TACATTGAAT ATAGATCACA TGGAACAGAA GGATCTGCAG
    CTCGACGAGA AGCTCCACCA CTCTGTTCTT CAGACACCAG ATGATCTAGA AATTAGTGAA
    TTTCCATCAG AATGTTGTAG TGTGATGGCA GGAGGTACTC TGACTGGATG GCATGCTGAT
    GTTGCTACTG TAATGTGGCG AAGAATGCTA GGCATTTTGG GAGATGTAAA TTCAATCATG
    GATCCTGAAA TACATGCTCA AGTTTTTGAT TACCTCTGTG AACTTTGGCA GAATCTAGCT
    AAGATTAGAG ATAACCTTGG CATTTCAACT GATAACCTGA CCTCCCCTTC TCCACCAGTT
    TTAATTCCTC CACTGAGAAT TCTTACACCT TGGCTTTTTA AGGCAACCAT GTTGACTGAT
    AAATATAAAC AAGGTAAATT ACATGCATAT AAACTTATTT GTAATACAAT GAAAAGAAGA
    CAAGATGTTT CTCCAAATAG AGATTTTCTA ACACATTTCT ACAATATAAT GCATTGTGGA
    TTACTTCATA TTGACCAGGA TATTGTCAAT ACAATCATCA AACACTGCTC ACCTCAATTT
    TTTTCACTTG GTTTGCCTGG TGCCACAATG CTTATTATGG ATTTTATTGT AGCAGCTGGT
    AGAGTGGCTT CTTCAGCTTT TCTCAATGCA CCAAGAGTAG AAGCACAAGT TCTTCTGGGA
    TCTTTGGTTT GCTTTCCCAA CTTATATTGT GAACTGCCTT CTCTTCATCC CAACATTCCT
    GATGTTGCTG TGTCTCAGTT TACAGATGTT AAGGAACTTA TAATCAAAAC TGTATTAAGC
    TCGGCAAGAG ATGAGCCCTC TGGTCCTGCA CGATGTGTAG CACTTTGTAG TTTAGGTATT
    TGGATTTGTG AAGAACTAGT CCATGAGTCT CATCATCCTC AAATTAAGGA AGCTCTGAAT
    GTGATTTGTG TTTCCTTAAA GTTTACTAAT AAAACAGTAG CCCACGTAGC TTGTAACATG
    CTTCACATGC TGGTTCATTA TGTACCTAGA CTTCAGATTT ACCAGCCTGA TTCTCCCTTG
    AAAATTATTC AAATCCTAAT AGCTACCATC ACCCATCTTT TACCAAGTAC AGAGGCTTCA
    TCTTATGAAA TGGACAAGAG GTTGGTAGTA TCTTTACTTC TCTGCCTTCT GGACTGGATC
    ATGGCCTTAC CTCTAAAGAC ACTGCTCCAA CCATTTCATG CTACGGGAGC AGAAAGCGAT
    AAAACAGAAA AATCTGTTCT CAATTGCATT TATAAGGTTT TACATGGGTG TGTTTATGGA
    GCTCAGTGTT TTAGCAATCC AAGGTATTTT CCCATGAGCC TCTCTGATTT GGCATCTGTA
    GATTATGATC CTTTTATGCA TTTGGAAAGT CTGAAAGAGC CTGAGCCTCT GCACTCTCCT
    GACTCAGAAC GATCTTCTAA ACTCCAGCCA GTAACAGAAG TGAAAACTCA AATGCAGCAT
    GGATTAATCT CTATAGCAGC CCGCACTGTT ATTACACATC TGGTAAATCA CCTGGGCCAT
    TATCCAATGA GCGGTGGTCC TGCTATGCTA ACAAGTCAGG TGTGTGAAAA TCACGACAAT
    CATTACAGTG AAAGTACTGA ACTTTCTCCT GAACTCTTTG AGAGTCCAAA TATCCAGTTC
    TTTGTGTTAA ATAATACAAC CTTAGTGTCC TGTATCCAGA TCAGATCAGA AGAGAATATG
    CCTGGAGGAG GTTTATCTGC TGGCCTTGCA TCAGCCAATT CAAATGTCAG AATCATAGTA
    CGTGATCTCT CTGGAAAATA TTCATGGGAT TCTGCTATAC TGTATGGCCC ACCTCCTGTA
    AGTGGCTTGT CAGAACCTAC ATCTTTCATG CTTTCATTGT CTCACCAAGA GAAGCCAGAA
    GAGCCTCCGA CATCTAATGA ATGCTTAGAA GATATAACCG TAAAAGATGG ACTTTCTCTC
    CAGTTTAAAA GATTTAGAGA AACTGTACCA ACTTGGGATA CAATAAGAGA TGAAGAAGAT
    GTTCTTGATG AGCTCTTGCA GTATTTGGGT GTTACTAGTC CTGAATGCTT ACAGAGAACT
    GGAATCTCAC TTAATATTCC TGCTCCACAA CCTGTGTGCA TTTCTGAAAA ACAAGAAAAT
    GATGTTATTA ATGCTATCCT TAAGCAACAT ACAGAAGAAA AAGAATTTGT TGAGAAGCAC
    TTTAATGACT TAAACATGAA AGCTGTGGAA CAAGATGAAC CAATACCTCA AAAACCTCAG
    TCAGCATTTT ATTATTGCAG ATTGCTTCTT AGTATATTGG GAATGAATTC CTGGGACAAA
    CGGAGGAGCT TTCATCTCCT GAAGAAAAAT GAAAAGCTAC TTAGAGAACT TAGGAACTTG
    GATTCAAGGC AGTGCCGAGA GACACACAAG ATTGCAGTAT TTTATGTTGC TGAAGGACAA
    GAAGACAAAC ACTCCATTCT CACCAATACA GGAGGAAGTC AAGCATATGA AGATTTTGTA
    GCTGGTCTTG GTTGGGAGGT AAATCTTACA AACCATTGTG GTTTTATGGG AGGACTACAA
    AAAAACAAAA GCACTGGATT GACCACTCCA TATTTTGCTA CCTCTACAGT AGAGGTAATA
    TTTCACGTGT CAACAAGAAT GCCTTCTGAT TCTGATGATT CTTTGACCAA AAAATTGAGA
    CATTTGGGAA ATGATGAAGT GCACATTGTT TGGTCAGAGC ATACTAGAGA CTACAGGAGA
    GGAATTATTC CCACAGAATT TGGTGATGTC CTTATTGTAA TATATCCAAT GAAAAATCAC
    ATGTTCAGTA TTCAGATAAT GAAAAAACCA GAGGTTCCCT TCTTTGGTCC CCTTTTTGAT
    GGTGCTATTG TGAATGGAAA GGTTCTACCC ATTATGGTTA GAGCAACAGC TATAAATGCA
    AGCCGTGCTC TGAAATCTCT GATTCCATTG TATCAAAACT TCTATGAGGA GAGAGCACGA
    TACCTGCAAA CAATTGTCCA GCACCACTTA GAACCAACAA CATTTGAAGA TTTTGCAGCA
    CAGGTTTTTT CTCCAGCTCC CTACCACCAT TTACCATCTG ATGCCGTTGG CTCCTACCCA
    GAGATTCTAC CCAGTGAAAC TCCCACAGCA ACGCAGGTAG ATGGGGCTGA CCTGGCCTCT
    CCAATGTCTC CTCGAACTAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG TTCCTCTGGC
    TCAGCCAATA AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84854
    Clone ID Related Accession (Same CDS sequence) XM_017021147.1
    Accession Version XM_017021147.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGAAGCC GATCCATTGG TGAATGTGCT CTGCCATCGG CCTATATACG CAGTGCTAAA 
    AGTGCTCCTG TTCTGATCCA TACTTCCAAA CCCTTCTTGC CTGATATTGT TCTCACTCCC
    CTTTCTGATG AGCTTTCAGA TATTGATGAT GCTCAAATAC TTCCCCGCTC AACTAGAGTC
    AGACATTTTT CACAAAGTGA AGAAACTGGA AATGAAGTTT TTGGTGCTTT GAATGAGGAG
    CAGCCATTGC CTCGAAGTAG CAGCACTTCT GACATCTTGG AACCATTCAC TGTTGAACGA
    GCCAAAGTCA ATAAAGAGGA CATGAGCCAA AAACTGCCTC CTCTTAATAG TGATATTGGC
    GGCAGCAGTG CTAATGTTCC TGATCTGATG GATGAGTTTA TAGCAGAACG ACTTCGAAGT
    GGTAATGCCT CGACTATGAC AAGAAGAGGA AGTAGTCCAG GCAGCCTGGA AATTCCCAAA
    GACCTCCCTG ATATTCTAAA CAAGCAGAAC CAGATGCGCC CTATTGATGA CCCAGGTGTG
    CCCTCAGAAT GGACTTCTCC TGCCAGTGCA GGGAGCAGTG ATCTTATCAG CTCAGATAGT
    CATTCGGATT CTTTCAGCGC TTTCCAATAT GATGGCCGAA AATTTGACAA TTTTGGCTTT
    GGAACCGACA CTGGGGTTAC GTCCTCTGCT GATGTGGATT CAGGTTCTGG CCATCATCAG
    AGTGCTGAAG AGCAGGAAGT GGCTAGTCTA ACTACTCTTC ATATAGATTC TGAAACAAGC
    AGTCTTAATC AGCAAGCTTT CTCTGCTGAA GTTGCAACTA TTACTGGTTC AGAAAGTGCT
    TCCCCAGTCC ACTCACCTCT GGGCTCCAGG TCACAGACTC CTTCCCCTTC TACATTGAAT
    ATAGATCACA TGGAACAGAA GGATCTGCAG CTCGACGAGA AGCTCCACCA CTCTGTTCTT
    CAGACACCAG ATGATCTAGA AATTAGTGAA TTTCCATCAG AATGTTGTAG TGTGATGGCA
    GGAGGTACTC TGACTGGATG GCATGCTGAT GTTGCTACTG TAATGTGGCG AAGAATGCTA
    GGCATTTTGG GAGATGTAAA TTCAATCATG GATCCTGAAA TACATGCTCA AGTTTTTGAT
    TACCTCTGTG AACTTTGGCA GAATCTAGCT AAGATTAGAG ATAACCTTGG CATTTCAACT
    GATAACCTGA CCTCCCCTTC TCCACCAGTT TTAATTCCTC CACTGAGAAT TCTTACACCT
    TGGCTTTTTA AGGCAACCAT GTTGACTGAT AAATATAAAC AAGGTAAATT ACATGCATAT
    AAACTTATTT GTAATACAAT GAAAAGAAGA CAAGATGTTT CTCCAAATAG AGATTTTCTA
    ACACATTTCT ACAATATAAT GCATTGTGGA TTACTTCATA TTGACCAGGA TATTGTCAAT
    ACAATCATCA AACACTGCTC ACCTCAATTT TTTTCACTTG GTTTGCCTGG TGCCACAATG
    CTTATTATGG ATTTTATTGT AGCAGCTGGT AGAGTGGCTT CTTCAGCTTT TCTCAATGCA
    CCAAGAGTAG AAGCACAAGT TCTTCTGGGA TCTTTGGTTT GCTTTCCCAA CTTATATTGT
    GAACTGCCTT CTCTTCATCC CAACATTCCT GATGTTGCTG TGTCTCAGTT TACAGATGTT
    AAGGAACTTA TAATCAAAAC TGTATTAAGC TCGGCAAGAG ATGAGCCCTC TGGTCCTGCA
    CGATGTGTAG CACTTTGTAG TTTAGGTATT TGGATTTGTG AAGAACTAGT CCATGAGTCT
    CATCATCCTC AAATTAAGGA AGCTCTGAAT GTGATTTGTG TTTCCTTAAA GTTTACTAAT
    AAAACAGTAG CCCACGTAGC TTGTAACATG CTTCACATGC TGGTTCATTA TGTACCTAGA
    CTTCAGATTT ACCAGCCTGA TTCTCCCTTG AAAATTATTC AAATCCTAAT AGCTACCATC
    ACCCATCTTT TACCAAGTAC AGAGGCTTCA TCTTATGAAA TGGACAAGAG GTTGGTAGTA
    TCTTTACTTC TCTGCCTTCT GGACTGGATC ATGGCCTTAC CTCTAAAGAC ACTGCTCCAA
    CCATTTCATG CTACGGGAGC AGAAAGCGAT AAAACAGAAA AATCTGTTCT CAATTGCATT
    TATAAGGTTT TACATGGGTG TGTTTATGGA GCTCAGTGTT TTAGCAATCC AAGGTATTTT
    CCCATGAGCC TCTCTGATTT GGCATCTGTA GATTATGATC CTTTTATGCA TTTGGAAAGT
    CTGAAAGAGC CTGAGCCTCT GCACTCTCCT GACTCAGAAC GATCTTCTAA ACTCCAGCCA
    GTAACAGAAG TGAAAACTCA AATGCAGCAT GGATTAATCT CTATAGCAGC CCGCACTGTT
    ATTACACATC TGGTAAATCA CCTGGGCCAT TATCCAATGA GCGGTGGTCC TGCTATGCTA
    ACAAGTCAGG TGTGTGAAAA TCACGACAAT CATTACAGTG AAAGTACTGA ACTTTCTCCT
    GAACTCTTTG AGAGTCCAAA TATCCAGTTC TTTGTGTTAA ATAATACAAC CTTAGTGTCC
    TGTATCCAGA TCAGATCAGA AGAGAATATG CCTGGAGGAG GTTTATCTGC TGGCCTTGCA
    TCAGCCAATT CAAATGTCAG AATCATAGTA CGTGATCTCT CTGGAAAATA TTCATGGGAT
    TCTGCTATAC TGTATGGCCC ACCTCCTGTA AGTGGCTTGT CAGAACCTAC ATCTTTCATG
    CTTTCATTGT CTCACCAAGA GAAGCCAGAA GAGCCTCCGA CATCTAATGA ATGCTTAGAA
    GATATAACCG TAAAAGATGG ACTTTCTCTC CAGTTTAAAA GATTTAGAGA AACTGTACCA
    ACTTGGGATA CAATAAGAGA TGAAGAAGAT GTTCTTGATG AGCTCTTGCA GTATTTGGGT
    GTTACTAGTC CTGAATGCTT ACAGAGAACT GGAATCTCAC TTAATATTCC TGCTCCACAA
    CCTGTGTGCA TTTCTGAAAA ACAAGAAAAT GATGTTATTA ATGCTATCCT TAAGCAACAT
    ACAGAAGAAA AAGAATTTGT TGAGAAGCAC TTTAATGACT TAAACATGAA AGCTGTGGAA
    CAAGATGAAC CAATACCTCA AAAACCTCAG TCAGCATTTT ATTATTGCAG ATTGCTTCTT
    AGTATATTGG GAATGAATTC CTGGGACAAA CGGAGGAGCT TTCATCTCCT GAAGAAAAAT
    GAAAAGCTAC TTAGAGAACT TAGGAACTTG GATTCAAGGC AGTGCCGAGA GACACACAAG
    ATTGCAGTAT TTTATGTTGC TGAAGGACAA GAAGACAAAC ACTCCATTCT CACCAATACA
    GGAGGAAGTC AAGCATATGA AGATTTTGTA GCTGGTCTTG GTTGGGAGGT AAATCTTACA
    AACCATTGTG GTTTTATGGG AGGACTACAA AAAAACAAAA GCACTGGATT GACCACTCCA
    TATTTTGCTA CCTCTACAGT AGAGGTAATA TTTCACGTGT CAACAAGAAT GCCTTCTGAT
    TCTGATGATT CTTTGACCAA AAAATTGAGA CATTTGGGAA ATGATGAAGT GCACATTGTT
    TGGTCAGAGC ATACTAGAGA CTACAGGAGA GGAATTATTC CCACAGAATT TGGTGATGTC
    CTTATTGTAA TATATCCAAT GAAAAATCAC ATGTTCAGTA TTCAGATAAT GAAAAAACCA
    GAGGTTCCCT TCTTTGGTCC CCTTTTTGAT GGTGCTATTG TGAATGGAAA GGTTCTACCC
    ATTATGGTTA GAGCAACAGC TATAAATGCA AGCCGTGCTC TGAAATCTCT GATTCCATTG
    TATCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTCCA GCACCACTTA
    GAACCAACAA CATTTGAAGA TTTTGCAGCA CAGGTTTTTT CTCCAGCTCC CTACCACCAT
    TTACCATCTG ATGCCGATCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876636.1
    CDS670..4653
    Translation

    Target ORF information:

    RefSeq Version XM_017021147.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGAAGCC GATCCATTGG TGAATGTGCT CTGCCATCGG CCTATATACG CAGTGCTAAA 
    AGTGCTCCTG TTCTGATCCA TACTTCCAAA CCCTTCTTGC CTGATATTGT TCTCACTCCC
    CTTTCTGATG AGCTTTCAGA TATTGATGAT GCTCAAATAC TTCCCCGCTC AACTAGAGTC
    AGACATTTTT CACAAAGTGA AGAAACTGGA AATGAAGTTT TTGGTGCTTT GAATGAGGAG
    CAGCCATTGC CTCGAAGTAG CAGCACTTCT GACATCTTGG AACCATTCAC TGTTGAACGA
    GCCAAAGTCA ATAAAGAGGA CATGAGCCAA AAACTGCCTC CTCTTAATAG TGATATTGGC
    GGCAGCAGTG CTAATGTTCC TGATCTGATG GATGAGTTTA TAGCAGAACG ACTTCGAAGT
    GGTAATGCCT CGACTATGAC AAGAAGAGGA AGTAGTCCAG GCAGCCTGGA AATTCCCAAA
    GACCTCCCTG ATATTCTAAA CAAGCAGAAC CAGATGCGCC CTATTGATGA CCCAGGTGTG
    CCCTCAGAAT GGACTTCTCC TGCCAGTGCA GGGAGCAGTG ATCTTATCAG CTCAGATAGT
    CATTCGGATT CTTTCAGCGC TTTCCAATAT GATGGCCGAA AATTTGACAA TTTTGGCTTT
    GGAACCGACA CTGGGGTTAC GTCCTCTGCT GATGTGGATT CAGGTTCTGG CCATCATCAG
    AGTGCTGAAG AGCAGGAAGT GGCTAGTCTA ACTACTCTTC ATATAGATTC TGAAACAAGC
    AGTCTTAATC AGCAAGCTTT CTCTGCTGAA GTTGCAACTA TTACTGGTTC AGAAAGTGCT
    TCCCCAGTCC ACTCACCTCT GGGCTCCAGG TCACAGACTC CTTCCCCTTC TACATTGAAT
    ATAGATCACA TGGAACAGAA GGATCTGCAG CTCGACGAGA AGCTCCACCA CTCTGTTCTT
    CAGACACCAG ATGATCTAGA AATTAGTGAA TTTCCATCAG AATGTTGTAG TGTGATGGCA
    GGAGGTACTC TGACTGGATG GCATGCTGAT GTTGCTACTG TAATGTGGCG AAGAATGCTA
    GGCATTTTGG GAGATGTAAA TTCAATCATG GATCCTGAAA TACATGCTCA AGTTTTTGAT
    TACCTCTGTG AACTTTGGCA GAATCTAGCT AAGATTAGAG ATAACCTTGG CATTTCAACT
    GATAACCTGA CCTCCCCTTC TCCACCAGTT TTAATTCCTC CACTGAGAAT TCTTACACCT
    TGGCTTTTTA AGGCAACCAT GTTGACTGAT AAATATAAAC AAGGTAAATT ACATGCATAT
    AAACTTATTT GTAATACAAT GAAAAGAAGA CAAGATGTTT CTCCAAATAG AGATTTTCTA
    ACACATTTCT ACAATATAAT GCATTGTGGA TTACTTCATA TTGACCAGGA TATTGTCAAT
    ACAATCATCA AACACTGCTC ACCTCAATTT TTTTCACTTG GTTTGCCTGG TGCCACAATG
    CTTATTATGG ATTTTATTGT AGCAGCTGGT AGAGTGGCTT CTTCAGCTTT TCTCAATGCA
    CCAAGAGTAG AAGCACAAGT TCTTCTGGGA TCTTTGGTTT GCTTTCCCAA CTTATATTGT
    GAACTGCCTT CTCTTCATCC CAACATTCCT GATGTTGCTG TGTCTCAGTT TACAGATGTT
    AAGGAACTTA TAATCAAAAC TGTATTAAGC TCGGCAAGAG ATGAGCCCTC TGGTCCTGCA
    CGATGTGTAG CACTTTGTAG TTTAGGTATT TGGATTTGTG AAGAACTAGT CCATGAGTCT
    CATCATCCTC AAATTAAGGA AGCTCTGAAT GTGATTTGTG TTTCCTTAAA GTTTACTAAT
    AAAACAGTAG CCCACGTAGC TTGTAACATG CTTCACATGC TGGTTCATTA TGTACCTAGA
    CTTCAGATTT ACCAGCCTGA TTCTCCCTTG AAAATTATTC AAATCCTAAT AGCTACCATC
    ACCCATCTTT TACCAAGTAC AGAGGCTTCA TCTTATGAAA TGGACAAGAG GTTGGTAGTA
    TCTTTACTTC TCTGCCTTCT GGACTGGATC ATGGCCTTAC CTCTAAAGAC ACTGCTCCAA
    CCATTTCATG CTACGGGAGC AGAAAGCGAT AAAACAGAAA AATCTGTTCT CAATTGCATT
    TATAAGGTTT TACATGGGTG TGTTTATGGA GCTCAGTGTT TTAGCAATCC AAGGTATTTT
    CCCATGAGCC TCTCTGATTT GGCATCTGTA GATTATGATC CTTTTATGCA TTTGGAAAGT
    CTGAAAGAGC CTGAGCCTCT GCACTCTCCT GACTCAGAAC GATCTTCTAA ACTCCAGCCA
    GTAACAGAAG TGAAAACTCA AATGCAGCAT GGATTAATCT CTATAGCAGC CCGCACTGTT
    ATTACACATC TGGTAAATCA CCTGGGCCAT TATCCAATGA GCGGTGGTCC TGCTATGCTA
    ACAAGTCAGG TGTGTGAAAA TCACGACAAT CATTACAGTG AAAGTACTGA ACTTTCTCCT
    GAACTCTTTG AGAGTCCAAA TATCCAGTTC TTTGTGTTAA ATAATACAAC CTTAGTGTCC
    TGTATCCAGA TCAGATCAGA AGAGAATATG CCTGGAGGAG GTTTATCTGC TGGCCTTGCA
    TCAGCCAATT CAAATGTCAG AATCATAGTA CGTGATCTCT CTGGAAAATA TTCATGGGAT
    TCTGCTATAC TGTATGGCCC ACCTCCTGTA AGTGGCTTGT CAGAACCTAC ATCTTTCATG
    CTTTCATTGT CTCACCAAGA GAAGCCAGAA GAGCCTCCGA CATCTAATGA ATGCTTAGAA
    GATATAACCG TAAAAGATGG ACTTTCTCTC CAGTTTAAAA GATTTAGAGA AACTGTACCA
    ACTTGGGATA CAATAAGAGA TGAAGAAGAT GTTCTTGATG AGCTCTTGCA GTATTTGGGT
    GTTACTAGTC CTGAATGCTT ACAGAGAACT GGAATCTCAC TTAATATTCC TGCTCCACAA
    CCTGTGTGCA TTTCTGAAAA ACAAGAAAAT GATGTTATTA ATGCTATCCT TAAGCAACAT
    ACAGAAGAAA AAGAATTTGT TGAGAAGCAC TTTAATGACT TAAACATGAA AGCTGTGGAA
    CAAGATGAAC CAATACCTCA AAAACCTCAG TCAGCATTTT ATTATTGCAG ATTGCTTCTT
    AGTATATTGG GAATGAATTC CTGGGACAAA CGGAGGAGCT TTCATCTCCT GAAGAAAAAT
    GAAAAGCTAC TTAGAGAACT TAGGAACTTG GATTCAAGGC AGTGCCGAGA GACACACAAG
    ATTGCAGTAT TTTATGTTGC TGAAGGACAA GAAGACAAAC ACTCCATTCT CACCAATACA
    GGAGGAAGTC AAGCATATGA AGATTTTGTA GCTGGTCTTG GTTGGGAGGT AAATCTTACA
    AACCATTGTG GTTTTATGGG AGGACTACAA AAAAACAAAA GCACTGGATT GACCACTCCA
    TATTTTGCTA CCTCTACAGT AGAGGTAATA TTTCACGTGT CAACAAGAAT GCCTTCTGAT
    TCTGATGATT CTTTGACCAA AAAATTGAGA CATTTGGGAA ATGATGAAGT GCACATTGTT
    TGGTCAGAGC ATACTAGAGA CTACAGGAGA GGAATTATTC CCACAGAATT TGGTGATGTC
    CTTATTGTAA TATATCCAAT GAAAAATCAC ATGTTCAGTA TTCAGATAAT GAAAAAACCA
    GAGGTTCCCT TCTTTGGTCC CCTTTTTGAT GGTGCTATTG TGAATGGAAA GGTTCTACCC
    ATTATGGTTA GAGCAACAGC TATAAATGCA AGCCGTGCTC TGAAATCTCT GATTCCATTG
    TATCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTCCA GCACCACTTA
    GAACCAACAA CATTTGAAGA TTTTGCAGCA CAGGTTTTTT CTCCAGCTCC CTACCACCAT
    TTACCATCTG ATGCCGATCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu78242
    Clone ID Related Accession (Same CDS sequence) XM_024449523.1
    Accession Version XM_024449523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGTAATA TCGCACCTAA CCAGTCAGAC AGTTTTTTTA GAACACAAAC TTCTGAAAAA 
    TCTAAGCAAC TAAACACTGA TAAACAGCCA TCAGAGCCTA GTTTAGATAG CCCTTGTGAT
    AAAGAAAAAA GGAAACATCT GTACAGACAA GCAGCCACAG AATTAGATGC TTGTGTTGAT
    GTAACACTAG TTGAAAAGCT TAAGAGTGTG CAAATTAATG AAAAAATCAC TGTCCCACAT
    GTTATGCGTG CCAAGAAAGC AACACTAAAA GCACCTGTTA ACCGCAGAAT GCCTCATGTT
    ACAAGTACTA GCAAACTTTC TCCAACTAAA AGGAGCTTGT CTGAAACAGT AACTCATAGA
    GCCAAAATCA TGAAAATTGC TACTAAAAAA CGAAATAGTG TTCATGTTAC TTTTAGGCCA
    TCCACTGAGT CCGTTCAGTT TTATAATCCT CTGGAAAACA AAGAGGCTCC ATGGAAGATG
    AGACTGCGGA AGCTCGGTGG CTTTAGTAGT GGTAGCAGCA ATAGCAGCAC TAGCAACACC
    CATACCAGCA CAAATAGTGC TACAGAGCTA GTAAAACCTG GTGTGTACAG ACCTCTAGAT
    ACACTTGGTA CTGCATCAGT AAGCAGCAAA ACAGTGAAGG AATCCACAGA GATTCCCACC
    ACCATATTAC AAAAAGAAGG AATTGCAAGT AGTCAATTAG GTAGTCGTAG TACTCTTAGG
    TCATCAAGTC ATGAGGCAGG ACTACAGCAG GGCTCCCTGG GTGGCGTTTA TAAAACTGTT
    GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA
    CCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT
    GCAGTCAATA AAGAGGACAT GAGCCAAAAA CTGCCTCCTC TTAATAGTGA TATTGGCGGC
    AGCAGTGCTA ATGTTCCTGA TCTGATGGAT GAGTTTATAG CAGAACGACT TCGAAGTGGT
    AATGCCTCGA CTATGACAAG AAGAGGAAGT AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC
    CTCCCTGATA TTCTAAACAA GCAGAACCAG ATGCGCCCTA TTGATGACCC AGGTGTGCCC
    TCAGAATGGA CTTCTCCTGC CAGTGCAGGG AGCAGTGATC TTATCAGCTC AGATAGTCAT
    TCGGATTCTT TCAGCGCTTT CCAATATGAT GGCCGAAAAT TTGACAATTT TGGCTTTGGA
    ACCGACACTG GGGTTACGTC CTCTGCTGAT GTGGATTCAG GTTCTGGCCA TCATCAGAGT
    GCTGAAGAGC AGGAAGTGGC TAGTCTAACT ACTCTTCATA TAGATTCTGA AACAAGCAGT
    CTTAATCAGC AAGCTTTCTC TGCTGAAGTT GCAACTATTA CTGGTTCAGA AAGTGCTTCC
    CCAGTCCACT CACCTCTGGG CTCCAGGTCA CAGACTCCTT CCCCTTCTAC ATTGAATATA
    GATCACATGG AACAGAAGGA TCTGCAGCTC GACGAGAAGC TCCACCACTC TGTTCTTCAG
    ACACCAGATG ATCTAGAAAT TAGTGAATTT CCATCAGAAT GTTGTAGTGT GATGGCAGGA
    GGTACTCTGA CTGGATGGCA TGCTGATGTT GCTACTGTAA TGTGGCGAAG AATGCTAGGC
    ATTTTGGGAG ATGTAAATTC AATCATGGAT CCTGAAATAC ATGCTCAAGT TTTTGATTAC
    CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG ATTAGAGATA ACCTTGGCAT TTCAACTGAT
    AACCTGACCT CCCCTTCTCC ACCAGTTTTA ATTCCTCCAC TGAGAATTCT TACACCTTGG
    CTTTTTAAGG CAACCATGTT GACTGATAAA TATAAACAAG GTAAATTACA TGCATATAAA
    CTTATTTGTA ATACAATGAA AAGAAGACAA GATGTTTCTC CAAATAGAGA TTTTCTAACA
    CATTTCTACA ATATAATGCA TTGTGGATTA CTTCATATTG ACCAGGATAT TGTCAATACA
    ATCATCAAAC ACTGCTCACC TCAATTTTTT TCACTTGGTT TGCCTGGTGC CACAATGCTT
    ATTATGGATT TTATTGTAGC AGCTGGTAGA GTGGCTTCTT CAGCTTTTCT CAATGCACCA
    AGAGTAGAAG CACAAGTTCT TCTGGGATCT TTGGTTTGCT TTCCCAACTT ATATTGTGAA
    CTGCCTTCTC TTCATCCCAA CATTCCTGAT GTTGCTGTGT CTCAGTTTAC AGATGTTAAG
    GAACTTATAA TCAAAACTGT ATTAAGCTCG GCAAGAGATG AGCCCTCTGG TCCTGCACGA
    TGTGTAGCAC TTTGTAGTTT AGGTATTTGG ATTTGTGAAG AACTAGTCCA TGAGTCTCAT
    CATCCTCAAA TTAAGGAAGC TCTGAATGTG ATTTGTGTTT CCTTAAAGTT TACTAATAAA
    ACAGTAGCCC ACGTAGCTTG TAACATGCTT CACATGCTGG TTCATTATGT ACCTAGACTT
    CAGATTTACC AGCCTGATTC TCCCTTGAAA ATTATTCAAA TCCTAATAGC TACCATCACC
    CATCTTTTAC CAAGTACAGA GGCTTCATCT TATGAAATGG ACAAGAGGTT GGTAGTATCT
    TTACTTCTCT GCCTTCTGGA CTGGATCATG GCCTTACCTC TAAAGACACT GCTCCAACCA
    TTTCATGCTA CGGGAGCAGA AAGCGATAAA ACAGAAAAAT CTGTTCTCAA TTGCATTTAT
    AAGGTTTTAC ATGGGTGTGT TTATGGAGCT CAGTGTTTTA GCAATCCAAG GTATTTTCCC
    ATGAGCCTCT CTGATTTGGC ATCTGTAGAT TATGATCCTT TTATGCATTT GGAAAGTCTG
    AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC TCAGAACGAT CTTCTAAACT CCAGCCAGTA
    ACAGAAGTGA AAACTCAAAT GCAGCATGGA TTAATCTCTA TAGCAGCCCG CACTGTTATT
    ACACATCTGG TAAATCACCT GGGCCATTAT CCAATGAGCG GTGGTCCTGC TATGCTAACA
    AGTCAGGTGT GTGAAAATCA CGACAATCAT TACAGTGAAA GTACTGAACT TTCTCCTGAA
    CTCTTTGAGA GTCCAAATAT CCAGTTCTTT GTGTTAAATA ATACAACCTT AGTGTCCTGT
    ATCCAGATCA GATCAGAAGA GAATATGCCT GGAGGAGGTT TATCTGCTGG CCTTGCATCA
    GCCAATTCAA ATGTCAGAAT CATAGTACGT GATCTCTCTG GAAAATATTC ATGGGATTCT
    GCTATACTGT ATGGCCCACC TCCTGTAAGT GGCTTGTCAG AACCTACATC TTTCATGCTT
    TCATTGTCTC ACCAAGAGAA GCCAGAAGAG CCTCCGACAT CTAATGAATG CTTAGAAGAT
    ATAACCGTAA AAGATGGACT TTCTCTCCAG TTTAAAAGAT TTAGAGAAAC TGTACCAACT
    TGGGATACAA TAAGAGATGA AGAAGATGTT CTTGATGAGC TCTTGCAGTA TTTGGGTGTT
    ACTAGTCCTG AATGCTTACA GAGAACTGGA ATCTCACTTA ATATTCCTGC TCCACAACCT
    GTGTGCATTT CTGAAAAACA AGAAAATGAT GTTATTAATG CTATCCTTAA GCAACATACA
    GAAGAAAAAG AATTTGTTGA GAAGCACTTT AATGACTTAA ACATGAAAGC TGTGGAACAA
    GATGAACCAA TACCTCAAAA ACCTCAGTCA GCATTTTATT ATTGCAGATT GCTTCTTAGT
    ATATTGGGAA TGAATTCCTG GGACAAACGG AGGAGCTTTC ATCTCCTGAA GAAAAATGAA
    AAGCTACTTA GAGAACTTAG GAACTTGGAT TCAAGGCAGT GCCGAGAGAC ACACAAGATT
    GCAGTATTTT ATGTTGCTGA AGGACAAGAA GACAAACACT CCATTCTCAC CAATACAGGA
    GGAAGTCAAG CATATGAAGA TTTTGTAGCT GGTCTTGGTT GGGAGGTAAA TCTTACAAAC
    CATTGTGGTT TTATGGGAGG ACTACAAAAA AACAAAAGCA CTGGATTGAC CACTCCATAT
    TTTGCTACCT CTACAGTAGA GGTAATATTT CACGTGTCAA CAAGAATGCC TTCTGATTCT
    GATGATTCTT TGACCAAAAA ATTGAGACAT TTGGGAAATG ATGAAGTGCA CATTGTTTGG
    TCAGAGCATA CTAGAGACTA CAGGAGAGGA ATTATTCCCA CAGAATTTGG TGATGTCCTT
    ATTGTAATAT ATCCAATGAA AAATCACATG TTCAGTATTC AGATAATGAA AAAACCAGAG
    GTTCCCTTCT TTGGTCCCCT TTTTGATGGT GCTATTGTGA ATGGAAAGGT TCTACCCATT
    ATGGTTAGAG CAACAGCTAT AAATGCAAGC CGTGCTCTGA AATCTCTGAT TCCATTGTAT
    CAAAACTTCT ATGAGGAGAG AGCACGATAC CTGCAAACAA TTGTCCAGCA CCACTTAGAA
    CCAACAACAT TTGAAGATTT TGCAGCACAG GTTTTTTCTC CAGCTCCCTA CCACCATTTA
    CCATCTGATG CCGTTGGCTC CTACCCAGAG ATTCTACCCA GTGAAACTCC CACAGCAACG
    CAGGTAGATG GGGCTGACCT GGCCTCTCCA ATGTCTCCTC GAACTAGCAA AAGCCGCATG
    TCCATGAAGC TGCGTCGTTC CTCTGGCTCA GCCAATAAAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024305291.1
    CDS1046..5770
    Translation

    Target ORF information:

    RefSeq Version XM_024449523.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024449523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGTAATA TCGCACCTAA CCAGTCAGAC AGTTTTTTTA GAACACAAAC TTCTGAAAAA 
    TCTAAGCAAC TAAACACTGA TAAACAGCCA TCAGAGCCTA GTTTAGATAG CCCTTGTGAT
    AAAGAAAAAA GGAAACATCT GTACAGACAA GCAGCCACAG AATTAGATGC TTGTGTTGAT
    GTAACACTAG TTGAAAAGCT TAAGAGTGTG CAAATTAATG AAAAAATCAC TGTCCCACAT
    GTTATGCGTG CCAAGAAAGC AACACTAAAA GCACCTGTTA ACCGCAGAAT GCCTCATGTT
    ACAAGTACTA GCAAACTTTC TCCAACTAAA AGGAGCTTGT CTGAAACAGT AACTCATAGA
    GCCAAAATCA TGAAAATTGC TACTAAAAAA CGAAATAGTG TTCATGTTAC TTTTAGGCCA
    TCCACTGAGT CCGTTCAGTT TTATAATCCT CTGGAAAACA AAGAGGCTCC ATGGAAGATG
    AGACTGCGGA AGCTCGGTGG CTTTAGTAGT GGTAGCAGCA ATAGCAGCAC TAGCAACACC
    CATACCAGCA CAAATAGTGC TACAGAGCTA GTAAAACCTG GTGTGTACAG ACCTCTAGAT
    ACACTTGGTA CTGCATCAGT AAGCAGCAAA ACAGTGAAGG AATCCACAGA GATTCCCACC
    ACCATATTAC AAAAAGAAGG AATTGCAAGT AGTCAATTAG GTAGTCGTAG TACTCTTAGG
    TCATCAAGTC ATGAGGCAGG ACTACAGCAG GGCTCCCTGG GTGGCGTTTA TAAAACTGTT
    GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA
    CCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT
    GCAGTCAATA AAGAGGACAT GAGCCAAAAA CTGCCTCCTC TTAATAGTGA TATTGGCGGC
    AGCAGTGCTA ATGTTCCTGA TCTGATGGAT GAGTTTATAG CAGAACGACT TCGAAGTGGT
    AATGCCTCGA CTATGACAAG AAGAGGAAGT AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC
    CTCCCTGATA TTCTAAACAA GCAGAACCAG ATGCGCCCTA TTGATGACCC AGGTGTGCCC
    TCAGAATGGA CTTCTCCTGC CAGTGCAGGG AGCAGTGATC TTATCAGCTC AGATAGTCAT
    TCGGATTCTT TCAGCGCTTT CCAATATGAT GGCCGAAAAT TTGACAATTT TGGCTTTGGA
    ACCGACACTG GGGTTACGTC CTCTGCTGAT GTGGATTCAG GTTCTGGCCA TCATCAGAGT
    GCTGAAGAGC AGGAAGTGGC TAGTCTAACT ACTCTTCATA TAGATTCTGA AACAAGCAGT
    CTTAATCAGC AAGCTTTCTC TGCTGAAGTT GCAACTATTA CTGGTTCAGA AAGTGCTTCC
    CCAGTCCACT CACCTCTGGG CTCCAGGTCA CAGACTCCTT CCCCTTCTAC ATTGAATATA
    GATCACATGG AACAGAAGGA TCTGCAGCTC GACGAGAAGC TCCACCACTC TGTTCTTCAG
    ACACCAGATG ATCTAGAAAT TAGTGAATTT CCATCAGAAT GTTGTAGTGT GATGGCAGGA
    GGTACTCTGA CTGGATGGCA TGCTGATGTT GCTACTGTAA TGTGGCGAAG AATGCTAGGC
    ATTTTGGGAG ATGTAAATTC AATCATGGAT CCTGAAATAC ATGCTCAAGT TTTTGATTAC
    CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG ATTAGAGATA ACCTTGGCAT TTCAACTGAT
    AACCTGACCT CCCCTTCTCC ACCAGTTTTA ATTCCTCCAC TGAGAATTCT TACACCTTGG
    CTTTTTAAGG CAACCATGTT GACTGATAAA TATAAACAAG GTAAATTACA TGCATATAAA
    CTTATTTGTA ATACAATGAA AAGAAGACAA GATGTTTCTC CAAATAGAGA TTTTCTAACA
    CATTTCTACA ATATAATGCA TTGTGGATTA CTTCATATTG ACCAGGATAT TGTCAATACA
    ATCATCAAAC ACTGCTCACC TCAATTTTTT TCACTTGGTT TGCCTGGTGC CACAATGCTT
    ATTATGGATT TTATTGTAGC AGCTGGTAGA GTGGCTTCTT CAGCTTTTCT CAATGCACCA
    AGAGTAGAAG CACAAGTTCT TCTGGGATCT TTGGTTTGCT TTCCCAACTT ATATTGTGAA
    CTGCCTTCTC TTCATCCCAA CATTCCTGAT GTTGCTGTGT CTCAGTTTAC AGATGTTAAG
    GAACTTATAA TCAAAACTGT ATTAAGCTCG GCAAGAGATG AGCCCTCTGG TCCTGCACGA
    TGTGTAGCAC TTTGTAGTTT AGGTATTTGG ATTTGTGAAG AACTAGTCCA TGAGTCTCAT
    CATCCTCAAA TTAAGGAAGC TCTGAATGTG ATTTGTGTTT CCTTAAAGTT TACTAATAAA
    ACAGTAGCCC ACGTAGCTTG TAACATGCTT CACATGCTGG TTCATTATGT ACCTAGACTT
    CAGATTTACC AGCCTGATTC TCCCTTGAAA ATTATTCAAA TCCTAATAGC TACCATCACC
    CATCTTTTAC CAAGTACAGA GGCTTCATCT TATGAAATGG ACAAGAGGTT GGTAGTATCT
    TTACTTCTCT GCCTTCTGGA CTGGATCATG GCCTTACCTC TAAAGACACT GCTCCAACCA
    TTTCATGCTA CGGGAGCAGA AAGCGATAAA ACAGAAAAAT CTGTTCTCAA TTGCATTTAT
    AAGGTTTTAC ATGGGTGTGT TTATGGAGCT CAGTGTTTTA GCAATCCAAG GTATTTTCCC
    ATGAGCCTCT CTGATTTGGC ATCTGTAGAT TATGATCCTT TTATGCATTT GGAAAGTCTG
    AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC TCAGAACGAT CTTCTAAACT CCAGCCAGTA
    ACAGAAGTGA AAACTCAAAT GCAGCATGGA TTAATCTCTA TAGCAGCCCG CACTGTTATT
    ACACATCTGG TAAATCACCT GGGCCATTAT CCAATGAGCG GTGGTCCTGC TATGCTAACA
    AGTCAGGTGT GTGAAAATCA CGACAATCAT TACAGTGAAA GTACTGAACT TTCTCCTGAA
    CTCTTTGAGA GTCCAAATAT CCAGTTCTTT GTGTTAAATA ATACAACCTT AGTGTCCTGT
    ATCCAGATCA GATCAGAAGA GAATATGCCT GGAGGAGGTT TATCTGCTGG CCTTGCATCA
    GCCAATTCAA ATGTCAGAAT CATAGTACGT GATCTCTCTG GAAAATATTC ATGGGATTCT
    GCTATACTGT ATGGCCCACC TCCTGTAAGT GGCTTGTCAG AACCTACATC TTTCATGCTT
    TCATTGTCTC ACCAAGAGAA GCCAGAAGAG CCTCCGACAT CTAATGAATG CTTAGAAGAT
    ATAACCGTAA AAGATGGACT TTCTCTCCAG TTTAAAAGAT TTAGAGAAAC TGTACCAACT
    TGGGATACAA TAAGAGATGA AGAAGATGTT CTTGATGAGC TCTTGCAGTA TTTGGGTGTT
    ACTAGTCCTG AATGCTTACA GAGAACTGGA ATCTCACTTA ATATTCCTGC TCCACAACCT
    GTGTGCATTT CTGAAAAACA AGAAAATGAT GTTATTAATG CTATCCTTAA GCAACATACA
    GAAGAAAAAG AATTTGTTGA GAAGCACTTT AATGACTTAA ACATGAAAGC TGTGGAACAA
    GATGAACCAA TACCTCAAAA ACCTCAGTCA GCATTTTATT ATTGCAGATT GCTTCTTAGT
    ATATTGGGAA TGAATTCCTG GGACAAACGG AGGAGCTTTC ATCTCCTGAA GAAAAATGAA
    AAGCTACTTA GAGAACTTAG GAACTTGGAT TCAAGGCAGT GCCGAGAGAC ACACAAGATT
    GCAGTATTTT ATGTTGCTGA AGGACAAGAA GACAAACACT CCATTCTCAC CAATACAGGA
    GGAAGTCAAG CATATGAAGA TTTTGTAGCT GGTCTTGGTT GGGAGGTAAA TCTTACAAAC
    CATTGTGGTT TTATGGGAGG ACTACAAAAA AACAAAAGCA CTGGATTGAC CACTCCATAT
    TTTGCTACCT CTACAGTAGA GGTAATATTT CACGTGTCAA CAAGAATGCC TTCTGATTCT
    GATGATTCTT TGACCAAAAA ATTGAGACAT TTGGGAAATG ATGAAGTGCA CATTGTTTGG
    TCAGAGCATA CTAGAGACTA CAGGAGAGGA ATTATTCCCA CAGAATTTGG TGATGTCCTT
    ATTGTAATAT ATCCAATGAA AAATCACATG TTCAGTATTC AGATAATGAA AAAACCAGAG
    GTTCCCTTCT TTGGTCCCCT TTTTGATGGT GCTATTGTGA ATGGAAAGGT TCTACCCATT
    ATGGTTAGAG CAACAGCTAT AAATGCAAGC CGTGCTCTGA AATCTCTGAT TCCATTGTAT
    CAAAACTTCT ATGAGGAGAG AGCACGATAC CTGCAAACAA TTGTCCAGCA CCACTTAGAA
    CCAACAACAT TTGAAGATTT TGCAGCACAG GTTTTTTCTC CAGCTCCCTA CCACCATTTA
    CCATCTGATG CCGTTGGCTC CTACCCAGAG ATTCTACCCA GTGAAACTCC CACAGCAACG
    CAGGTAGATG GGGCTGACCT GGCCTCTCCA ATGTCTCCTC GAACTAGCAA AAGCCGCATG
    TCCATGAAGC TGCGTCGTTC CTCTGGCTCA GCCAATAAAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84853
    Clone ID Related Accession (Same CDS sequence) XM_017021146.1
    Accession Version XM_017021146.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAGAAGCC GATCCATTGG TGAATGTGCT CTGCCATCGG CCTATATACG CAGTGCTAAA 
    AGTGCTCCTG TTCTGATCCA TACTTCCAAA CCCTTCTTGC CTGATATTGT TCTCACTCCC
    CTTTCTGATG AGCTTTCAGA TATTGATGAT GCTCAAATAC TTCCCCGCTC AACTAGAGTC
    AGACATTTTT CACAAAGTGA AGAAACTGGA AATGAAGTTT TTGGTGCTTT GAATGAGGAG
    CAGCCATTGC CTCGAAGTAG CAGCACTTCT GACATCTTGG AACCATTCAC TGTTGAACGA
    GCCAAAGGTG CAGTTCCTGT CATTGACAGT TCATCCCGTC ATGCACCAAG CTTGCAGAGT
    TCCACAGAGG CTTCTTCAAT AACTAGATCC ACTGAAAGCC ACATCACTGA TACTCATAGT
    AGAGAGTCTT CTCTGGAAGT TGGTGATAGC ATATATGACC ATCTTTGTCA TTTAATAGGT
    CCAGTAGAAC TTGCAGATTC AGCTTTTGAA CAAATCCAGT ACATTGACCT TGAAGGAGAT
    GATGATCTTC TTTCCACCCT GAAAGAATAT TTTAAGGAAA ACCAGGAAAA TCATAGTAAA
    AATGAGACAG GGAAAGACCC AGCTTCTCAG GAAGTGACTA TTGCAGTAAA TAGGGGTGAA
    AGATTATCCT TAGATAAATT AGAATGCACA GATCAGGAAA CTGAATCAGA AAATATCACC
    TCTTTTGTTG GGACTCCTGA AAACCTACAG TTTCAGAAAG AACCAAACTC AGCTGTCTTC
    ATGAGTAATA TCGCACCTAA CCAGTCAGAC AGTTTTTTTA GAACACAAAC TTCTGAAAAA
    TCTAAGCAAC TAAACACTGA TAAACAGCCA TCAGAGCCTA GTTTAGATAG CCCTTGTGAT
    AAAGAAAAAA GGAAACATCT GTACAGACAA GCAGCCACAG AATTAGATGC TTGTGTTGAT
    GTAACACTAG TTGAAAAGCT TAAGAGTGTG CAAATTAATG AAAAAATCAC TGTCCCACAT
    GTTATGCGTG CCAAGAAAGC AACACTAAAA GCACCTGTTA ACCGCAGAAT GCCTCATGTT
    ACAAGTACTA GCAAACTTTC TCCAACTAAA AGGAGCTTGT CTGAAACAGT AACTCATAGA
    GCCAAAATCA TGAAAATTGC TACTAAAAAA CGAAATAGTG TTCATGTTAC TTTTAGGCCA
    TCCACTGAGT CCGTTCAGTT TTATAATCCT CTGGAAAACA AAGAGGCTCC ATGGAAGATG
    AGACTGCGGA AGCTCGGTGG CTTTAGTAGT GGTAGCAGCA ATAGCAGCAC TAGCAACACC
    CATACCAGCA CAAATAGTGC TACAGAGCTA GTAAAACCTG GTGTGTACAG ACCTCTAGAT
    ACACTTGGTA CTGCATCAGT AAGCAGCAAA ACAGTGAAGG AATCCACAGA GATTCCCACC
    ACCATATTAC AAAAAGAAGG AATTGCAAGT AGTCAATTAG GTAGTCGTAG TACTCTTAGG
    TCATCAAGTC ATGAGGCAGG ACTACAGCAG GGCTCCCTGG GTGGCGTTTA TAAAACTGTT
    GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA
    CCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT
    GCAGTCAATA AAGAGGACAT GAGCCAAAAA CTGCCTCCTC TTAATAGTGA TATTGGCGGC
    AGCAGTGCTA ATGTTCCTGA TCTGATGGAT GAGTTTATAG CAGAACGACT TCGAAGTGGT
    AATGCCTCGA CTATGACAAG AAGAGGAAGT AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC
    CTCCCTGATA TTCTAAACAA GCAGAACCAG ATGCGCCCTA TTGATGACCC AGGTGTGCCC
    TCAGAATGGA CTTCTCCTGC CAGTGCAGGG AGCAGTGATC TTATCAGCTC AGATAGTCAT
    TCGGATTCTT TCAGCGCTTT CCAATATGAT GGCCGAAAAT TTGACAATTT TGGCTTTGGA
    ACCGACACTG GGGTTACGTC CTCTGCTGAT GTGGATTCAG GTTCTGGCCA TCATCAGAGT
    GCTGAAGAGC AGGAAGTGGC TAGTCTAACT ACTCTTCATA TAGATTCTGA AACAAGCAGT
    CTTAATCAGC AAGCTTTCTC TGCTGAAGTT GCAACTATTA CTGGTTCAGA AAGTGCTTCC
    CCAGTCCACT CACCTCTGGG CTCCAGGTCA CAGACTCCTT CCCCTTCTAC ATTGAATATA
    GATCACATGG AACAGAAGGA TCTGCAGCTC GACGAGAAGC TCCACCACTC TGTTCTTCAG
    ACACCAGATG ATCTAGAAAT TAGTGAATTT CCATCAGAAT GTTGTAGTGT GATGGCAGGA
    GGTACTCTGA CTGGATGGCA TGCTGATGTT GCTACTGTAA TGTGGCGAAG AATGCTAGGC
    ATTTTGGGAG ATGTAAATTC AATCATGGAT CCTGAAATAC ATGCTCAAGT TTTTGATTAC
    CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG ATTAGAGATA ACCTTGGCAT TTCAACTGAT
    AACCTGACCT CCCCTTCTCC ACCAGTTTTA ATTCCTCCAC TGAGAATTCT TACACCTTGG
    CTTTTTAAGG CAACCATGTT GACTGATAAA TATAAACAAG GTAAATTACA TGCATATAAA
    CTTATTTGTA ATACAATGAA AAGAAGACAA GATGTTTCTC CAAATAGAGA TTTTCTAACA
    CATTTCTACA ATATAATGCA TTGTGGATTA CTTCATATTG ACCAGGATAT TGTCAATACA
    ATCATCAAAC ACTGCTCACC TCAATTTTTT TCACTTGGTT TGCCTGGTGC CACAATGCTT
    ATTATGGATT TTATTGTAGC AGCTGGTAGA GTGGCTTCTT CAGCTTTTCT CAATGCACCA
    AGAGTAGAAG CACAAGTTCT TCTGGGATCT TTGGTTTGCT TTCCCAACTT ATATTGTGAA
    CTGCCTTCTC TTCATCCCAA CATTCCTGAT GTTGCTGTGT CTCAGTTTAC AGATGTTAAG
    GAACTTATAA TCAAAACTGT ATTAAGCTCG GCAAGAGATG AGCCCTCTGG TCCTGCACGA
    TGTGTAGCAC TTTGTAGTTT AGGTATTTGG ATTTGTGAAG AACTAGTCCA TGAGTCTCAT
    CATCCTCAAA TTAAGGAAGC TCTGAATGTG ATTTGTGTTT CCTTAAAGTT TACTAATAAA
    ACAGTAGCCC ACGTAGCTTG TAACATGCTT CACATGCTGG TTCATTATGT ACCTAGACTT
    CAGATTTACC AGCCTGATTC TCCCTTGAAA ATTATTCAAA TCCTAATAGC TACCATCACC
    CATCTTTTAC CAAGTACAGA GGCTTCATCT TATGAAATGG ACAAGAGGTT GGTAGTATCT
    TTACTTCTCT GCCTTCTGGA CTGGATCATG GCCTTACCTC TAAAGACACT GCTCCAACCA
    TTTCATGCTA CGGGAGCAGA AAGCGATAAA ACAGAAAAAT CTGTTCTCAA TTGCATTTAT
    AAGGTTTTAC ATGGGTGTGT TTATGGAGCT CAGTGTTTTA GCAATCCAAG GTATTTTCCC
    ATGAGCCTCT CTGATTTGGC ATCTGTAGAT TATGATCCTT TTATGCATTT GGAAAGTCTG
    AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC TCAGAACGAT CTTCTAAACT CCAGCCAGTA
    ACAGAAGTGA AAACTCAAAT GCAGCATGGA TTAATCTCTA TAGCAGCCCG CACTGTTATT
    ACACATCTGG TAAATCACCT GGGCCATTAT CCAATGAGCG GTGGTCCTGC TATGCTAACA
    AGTCAGGTGT GTGAAAATCA CGACAATCAT TACAGTGAAA GTACTGAACT TTCTCCTGAA
    CTCTTTGAGA GTCCAAATAT CCAGTTCTTT GTGTTAAATA ATACAACCTT AGTGTCCTGT
    ATCCAGATCA GATCAGAAGA GAATATGCCT GGAGGAGGTT TATCTGCTGG CCTTGCATCA
    GCCAATTCAA ATGTCAGAAT CATAGTACGT GATCTCTCTG GAAAATATTC ATGGGATTCT
    GCTATACTGT ATGGCCCACC TCCTGTAAGT GGCTTGTCAG AACCTACATC TTTCATGCTT
    TCATTGTCTC ACCAAGAGAA GCCAGAAGAG CCTCCGACAT CTAATGAATG CTTAGAAGAT
    ATAACCGTAA AAGATGGACT TTCTCTCCAG TTTAAAAGAT TTAGAGAAAC TGTACCAACT
    TGGGATACAA TAAGAGATGA AGAAGATGTT CTTGATGAGC TCTTGCAGTA TTTGGGTGTT
    ACTAGTCCTG AATGCTTACA GAGAACTGGA ATCTCACTTA ATATTCCTGC TCCACAACCT
    GTGTGCATTT CTGAAAAACA AGAAAATGAT GTTATTAATG CTATCCTTAA GCAACATACA
    GAAGAAAAAG AATTTGTTGA GAAGCACTTT AATGACTTAA ACATGAAAGC TGTGGAACAA
    GATGAACCAA TACCTCAAAA ACCTCAGTCA GCATTTTATT ATTGCAGATT GCTTCTTAGT
    ATATTGGGAA TGAATTCCTG GGACAAACGG AGGAGCTTTC ATCTCCTGAA GAAAAATGAA
    AAGCTACTTA GAGAACTTAG GAACTTGGAT TCAAGGCAGT GCCGAGAGAC ACACAAGATT
    GCAGTATTTT ATGTTGCTGA AGGACAAGAA GACAAACACT CCATTCTCAC CAATACAGGA
    GGAAGTCAAG CATATGAAGA TTTTGTAGCT GGTCTTGGTT GGGAGGTAAA TCTTACAAAC
    CATTGTGGTT TTATGGGAGG ACTACAAAAA AACAAAAGCA CTGGATTGAC CACTCCATAT
    TTTGCTACCT CTACAGTAGA GGTAATATTT CACGTGTCAA CAAGAATGCC TTCTGATTCT
    GATGATTCTT TGACCAAAAA ATTGAGACAT TTGGGAAATG ATGAAGTGCA CATTGTTTGG
    TCAGAGCATA CTAGAGACTA CAGGAGAGGA ATTATTCCCA CAGAATTTGG TGATGTCCTT
    ATTGTAATAT ATCCAATGAA AAATCACATG TTCAGTATTC AGATAATGAA AAAACCAGAG
    GTTCCCTTCT TTGGTCCCCT TTTTGATGGT GCTATTGTGA ATGGAAAGGT TCTACCCATT
    ATGGTTAGAG CAACAGCTAT AAATGCAAGC CGTGCTCTGA AATCTCTGAT TCCATTGTAT
    CAAAACTTCT ATGAGGAGAG AGCACGATAC CTGCAAACAA TTGTCCAGCA CCACTTAGAA
    CCAACAACAT TTGAAGATTT TGCAGCACAG GTTTTTTCTC CAGCTCCCTA CCACCATTTA
    CCATCTGATG CCGTTGGCTC CTACCCAGAG ATTCTACCCA GTGAAACTCC CACAGCAACG
    CAGGTAGATG GGGCTGACCT GGCCTCTCCA ATGTCTCCTC GAACTAGCAA AAGCCGCATG
    TCCATGAAGC TGCGTCGTTC CTCTGGCTCA GCCAATAAAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876635.1
    CDS407..5911
    Translation

    Target ORF information:

    RefSeq Version XM_017021146.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAGAAGCC GATCCATTGG TGAATGTGCT CTGCCATCGG CCTATATACG CAGTGCTAAA 
    AGTGCTCCTG TTCTGATCCA TACTTCCAAA CCCTTCTTGC CTGATATTGT TCTCACTCCC
    CTTTCTGATG AGCTTTCAGA TATTGATGAT GCTCAAATAC TTCCCCGCTC AACTAGAGTC
    AGACATTTTT CACAAAGTGA AGAAACTGGA AATGAAGTTT TTGGTGCTTT GAATGAGGAG
    CAGCCATTGC CTCGAAGTAG CAGCACTTCT GACATCTTGG AACCATTCAC TGTTGAACGA
    GCCAAAGGTG CAGTTCCTGT CATTGACAGT TCATCCCGTC ATGCACCAAG CTTGCAGAGT
    TCCACAGAGG CTTCTTCAAT AACTAGATCC ACTGAAAGCC ACATCACTGA TACTCATAGT
    AGAGAGTCTT CTCTGGAAGT TGGTGATAGC ATATATGACC ATCTTTGTCA TTTAATAGGT
    CCAGTAGAAC TTGCAGATTC AGCTTTTGAA CAAATCCAGT ACATTGACCT TGAAGGAGAT
    GATGATCTTC TTTCCACCCT GAAAGAATAT TTTAAGGAAA ACCAGGAAAA TCATAGTAAA
    AATGAGACAG GGAAAGACCC AGCTTCTCAG GAAGTGACTA TTGCAGTAAA TAGGGGTGAA
    AGATTATCCT TAGATAAATT AGAATGCACA GATCAGGAAA CTGAATCAGA AAATATCACC
    TCTTTTGTTG GGACTCCTGA AAACCTACAG TTTCAGAAAG AACCAAACTC AGCTGTCTTC
    ATGAGTAATA TCGCACCTAA CCAGTCAGAC AGTTTTTTTA GAACACAAAC TTCTGAAAAA
    TCTAAGCAAC TAAACACTGA TAAACAGCCA TCAGAGCCTA GTTTAGATAG CCCTTGTGAT
    AAAGAAAAAA GGAAACATCT GTACAGACAA GCAGCCACAG AATTAGATGC TTGTGTTGAT
    GTAACACTAG TTGAAAAGCT TAAGAGTGTG CAAATTAATG AAAAAATCAC TGTCCCACAT
    GTTATGCGTG CCAAGAAAGC AACACTAAAA GCACCTGTTA ACCGCAGAAT GCCTCATGTT
    ACAAGTACTA GCAAACTTTC TCCAACTAAA AGGAGCTTGT CTGAAACAGT AACTCATAGA
    GCCAAAATCA TGAAAATTGC TACTAAAAAA CGAAATAGTG TTCATGTTAC TTTTAGGCCA
    TCCACTGAGT CCGTTCAGTT TTATAATCCT CTGGAAAACA AAGAGGCTCC ATGGAAGATG
    AGACTGCGGA AGCTCGGTGG CTTTAGTAGT GGTAGCAGCA ATAGCAGCAC TAGCAACACC
    CATACCAGCA CAAATAGTGC TACAGAGCTA GTAAAACCTG GTGTGTACAG ACCTCTAGAT
    ACACTTGGTA CTGCATCAGT AAGCAGCAAA ACAGTGAAGG AATCCACAGA GATTCCCACC
    ACCATATTAC AAAAAGAAGG AATTGCAAGT AGTCAATTAG GTAGTCGTAG TACTCTTAGG
    TCATCAAGTC ATGAGGCAGG ACTACAGCAG GGCTCCCTGG GTGGCGTTTA TAAAACTGTT
    GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA
    CCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT
    GCAGTCAATA AAGAGGACAT GAGCCAAAAA CTGCCTCCTC TTAATAGTGA TATTGGCGGC
    AGCAGTGCTA ATGTTCCTGA TCTGATGGAT GAGTTTATAG CAGAACGACT TCGAAGTGGT
    AATGCCTCGA CTATGACAAG AAGAGGAAGT AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC
    CTCCCTGATA TTCTAAACAA GCAGAACCAG ATGCGCCCTA TTGATGACCC AGGTGTGCCC
    TCAGAATGGA CTTCTCCTGC CAGTGCAGGG AGCAGTGATC TTATCAGCTC AGATAGTCAT
    TCGGATTCTT TCAGCGCTTT CCAATATGAT GGCCGAAAAT TTGACAATTT TGGCTTTGGA
    ACCGACACTG GGGTTACGTC CTCTGCTGAT GTGGATTCAG GTTCTGGCCA TCATCAGAGT
    GCTGAAGAGC AGGAAGTGGC TAGTCTAACT ACTCTTCATA TAGATTCTGA AACAAGCAGT
    CTTAATCAGC AAGCTTTCTC TGCTGAAGTT GCAACTATTA CTGGTTCAGA AAGTGCTTCC
    CCAGTCCACT CACCTCTGGG CTCCAGGTCA CAGACTCCTT CCCCTTCTAC ATTGAATATA
    GATCACATGG AACAGAAGGA TCTGCAGCTC GACGAGAAGC TCCACCACTC TGTTCTTCAG
    ACACCAGATG ATCTAGAAAT TAGTGAATTT CCATCAGAAT GTTGTAGTGT GATGGCAGGA
    GGTACTCTGA CTGGATGGCA TGCTGATGTT GCTACTGTAA TGTGGCGAAG AATGCTAGGC
    ATTTTGGGAG ATGTAAATTC AATCATGGAT CCTGAAATAC ATGCTCAAGT TTTTGATTAC
    CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG ATTAGAGATA ACCTTGGCAT TTCAACTGAT
    AACCTGACCT CCCCTTCTCC ACCAGTTTTA ATTCCTCCAC TGAGAATTCT TACACCTTGG
    CTTTTTAAGG CAACCATGTT GACTGATAAA TATAAACAAG GTAAATTACA TGCATATAAA
    CTTATTTGTA ATACAATGAA AAGAAGACAA GATGTTTCTC CAAATAGAGA TTTTCTAACA
    CATTTCTACA ATATAATGCA TTGTGGATTA CTTCATATTG ACCAGGATAT TGTCAATACA
    ATCATCAAAC ACTGCTCACC TCAATTTTTT TCACTTGGTT TGCCTGGTGC CACAATGCTT
    ATTATGGATT TTATTGTAGC AGCTGGTAGA GTGGCTTCTT CAGCTTTTCT CAATGCACCA
    AGAGTAGAAG CACAAGTTCT TCTGGGATCT TTGGTTTGCT TTCCCAACTT ATATTGTGAA
    CTGCCTTCTC TTCATCCCAA CATTCCTGAT GTTGCTGTGT CTCAGTTTAC AGATGTTAAG
    GAACTTATAA TCAAAACTGT ATTAAGCTCG GCAAGAGATG AGCCCTCTGG TCCTGCACGA
    TGTGTAGCAC TTTGTAGTTT AGGTATTTGG ATTTGTGAAG AACTAGTCCA TGAGTCTCAT
    CATCCTCAAA TTAAGGAAGC TCTGAATGTG ATTTGTGTTT CCTTAAAGTT TACTAATAAA
    ACAGTAGCCC ACGTAGCTTG TAACATGCTT CACATGCTGG TTCATTATGT ACCTAGACTT
    CAGATTTACC AGCCTGATTC TCCCTTGAAA ATTATTCAAA TCCTAATAGC TACCATCACC
    CATCTTTTAC CAAGTACAGA GGCTTCATCT TATGAAATGG ACAAGAGGTT GGTAGTATCT
    TTACTTCTCT GCCTTCTGGA CTGGATCATG GCCTTACCTC TAAAGACACT GCTCCAACCA
    TTTCATGCTA CGGGAGCAGA AAGCGATAAA ACAGAAAAAT CTGTTCTCAA TTGCATTTAT
    AAGGTTTTAC ATGGGTGTGT TTATGGAGCT CAGTGTTTTA GCAATCCAAG GTATTTTCCC
    ATGAGCCTCT CTGATTTGGC ATCTGTAGAT TATGATCCTT TTATGCATTT GGAAAGTCTG
    AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC TCAGAACGAT CTTCTAAACT CCAGCCAGTA
    ACAGAAGTGA AAACTCAAAT GCAGCATGGA TTAATCTCTA TAGCAGCCCG CACTGTTATT
    ACACATCTGG TAAATCACCT GGGCCATTAT CCAATGAGCG GTGGTCCTGC TATGCTAACA
    AGTCAGGTGT GTGAAAATCA CGACAATCAT TACAGTGAAA GTACTGAACT TTCTCCTGAA
    CTCTTTGAGA GTCCAAATAT CCAGTTCTTT GTGTTAAATA ATACAACCTT AGTGTCCTGT
    ATCCAGATCA GATCAGAAGA GAATATGCCT GGAGGAGGTT TATCTGCTGG CCTTGCATCA
    GCCAATTCAA ATGTCAGAAT CATAGTACGT GATCTCTCTG GAAAATATTC ATGGGATTCT
    GCTATACTGT ATGGCCCACC TCCTGTAAGT GGCTTGTCAG AACCTACATC TTTCATGCTT
    TCATTGTCTC ACCAAGAGAA GCCAGAAGAG CCTCCGACAT CTAATGAATG CTTAGAAGAT
    ATAACCGTAA AAGATGGACT TTCTCTCCAG TTTAAAAGAT TTAGAGAAAC TGTACCAACT
    TGGGATACAA TAAGAGATGA AGAAGATGTT CTTGATGAGC TCTTGCAGTA TTTGGGTGTT
    ACTAGTCCTG AATGCTTACA GAGAACTGGA ATCTCACTTA ATATTCCTGC TCCACAACCT
    GTGTGCATTT CTGAAAAACA AGAAAATGAT GTTATTAATG CTATCCTTAA GCAACATACA
    GAAGAAAAAG AATTTGTTGA GAAGCACTTT AATGACTTAA ACATGAAAGC TGTGGAACAA
    GATGAACCAA TACCTCAAAA ACCTCAGTCA GCATTTTATT ATTGCAGATT GCTTCTTAGT
    ATATTGGGAA TGAATTCCTG GGACAAACGG AGGAGCTTTC ATCTCCTGAA GAAAAATGAA
    AAGCTACTTA GAGAACTTAG GAACTTGGAT TCAAGGCAGT GCCGAGAGAC ACACAAGATT
    GCAGTATTTT ATGTTGCTGA AGGACAAGAA GACAAACACT CCATTCTCAC CAATACAGGA
    GGAAGTCAAG CATATGAAGA TTTTGTAGCT GGTCTTGGTT GGGAGGTAAA TCTTACAAAC
    CATTGTGGTT TTATGGGAGG ACTACAAAAA AACAAAAGCA CTGGATTGAC CACTCCATAT
    TTTGCTACCT CTACAGTAGA GGTAATATTT CACGTGTCAA CAAGAATGCC TTCTGATTCT
    GATGATTCTT TGACCAAAAA ATTGAGACAT TTGGGAAATG ATGAAGTGCA CATTGTTTGG
    TCAGAGCATA CTAGAGACTA CAGGAGAGGA ATTATTCCCA CAGAATTTGG TGATGTCCTT
    ATTGTAATAT ATCCAATGAA AAATCACATG TTCAGTATTC AGATAATGAA AAAACCAGAG
    GTTCCCTTCT TTGGTCCCCT TTTTGATGGT GCTATTGTGA ATGGAAAGGT TCTACCCATT
    ATGGTTAGAG CAACAGCTAT AAATGCAAGC CGTGCTCTGA AATCTCTGAT TCCATTGTAT
    CAAAACTTCT ATGAGGAGAG AGCACGATAC CTGCAAACAA TTGTCCAGCA CCACTTAGAA
    CCAACAACAT TTGAAGATTT TGCAGCACAG GTTTTTTCTC CAGCTCCCTA CCACCATTTA
    CCATCTGATG CCGTTGGCTC CTACCCAGAG ATTCTACCCA GTGAAACTCC CACAGCAACG
    CAGGTAGATG GGGCTGACCT GGCCTCTCCA ATGTCTCCTC GAACTAGCAA AAGCCGCATG
    TCCATGAAGC TGCGTCGTTC CTCTGGCTCA GCCAATAAAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47636
    Clone ID Related Accession (Same CDS sequence) XM_006720104.4 , XM_006720104.3
    Accession Version XM_006720104.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006720104.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720167.1
    CDS176..4999
    Translation

    Target ORF information:

    RefSeq Version XM_006720104.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720104.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47636
    Clone ID Related Accession (Same CDS sequence) XM_006720104.4 , XM_006720104.3
    Accession Version XM_006720104.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006720104.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720167.1
    CDS470..5293
    Translation

    Target ORF information:

    RefSeq Version XM_006720104.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720104.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu78241
    Clone ID Related Accession (Same CDS sequence) XM_011536616.2 , XM_011536616.3
    Accession Version XM_011536616.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536616.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGTC CACACAATTG
    TTAAAGAACA TTAGCCATAT CCTCCAGACA CAAAGGCTTG AATGTTCATT TGGAGCATAT
    CCTTTTTTGT GTTTAAAACT GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534918.1
    CDS470..5896
    Translation

    Target ORF information:

    RefSeq Version XM_011536616.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011536616.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGTC CACACAATTG
    TTAAAGAACA TTAGCCATAT CCTCCAGACA CAAAGGCTTG AATGTTCATT TGGAGCATAT
    CCTTTTTTGT GTTTAAAACT GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu78241
    Clone ID Related Accession (Same CDS sequence) XM_011536616.2 , XM_011536616.3
    Accession Version XM_011536616.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011536616.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGTC CACACAATTG
    TTAAAGAACA TTAGCCATAT CCTCCAGACA CAAAGGCTTG AATGTTCATT TGGAGCATAT
    CCTTTTTTGT GTTTAAAACT GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534918.1
    CDS176..5602
    Translation

    Target ORF information:

    RefSeq Version XM_011536616.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011536616.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGTC CACACAATTG
    TTAAAGAACA TTAGCCATAT CCTCCAGACA CAAAGGCTTG AATGTTCATT TGGAGCATAT
    CCTTTTTTGT GTTTAAAACT GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102383
    Clone ID Related Accession (Same CDS sequence) NM_001330075.2 , NM_001346248.2 , NM_001346248.1 , NM_001330075.3
    Accession Version NM_001330075.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Oct 4, 2016 this sequence version replaced NM_001330075.1. Transcript Variant: This variant (5) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (5) is longer than isoform 1. Variants 5 and 10 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.131652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317004.1
    CDS489..7976
    Translation

    Target ORF information:

    RefSeq Version NM_001330075.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330075.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102383
    Clone ID Related Accession (Same CDS sequence) NM_001330075.2 , NM_001346248.2 , NM_001346248.1 , NM_001330075.3
    Accession Version NM_001346248.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Jun 2, 2019 this sequence version replaced NM_001346248.1. Transcript Variant: This variant (10) differs in the 3' UTR, and uses an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (5) is longer than isoform 1. Variants 5 and 10 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.43817.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333177.1
    CDS461..7948
    Translation

    Target ORF information:

    RefSeq Version NM_001346248.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346248.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102383
    Clone ID Related Accession (Same CDS sequence) NM_001330075.2 , NM_001346248.2 , NM_001346248.1 , NM_001330075.3
    Accession Version NM_001346248.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-14
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Oct 4, 2016 this sequence version replaced XM_017021141.1. Transcript Variant: This variant (10) differs in the 3' UTR, and uses an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (5) is longer than isoform 1. Variants 5 and 10 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.43817.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333177.1
    CDS489..7976
    Translation

    Target ORF information:

    RefSeq Version NM_001346248.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102383
    Clone ID Related Accession (Same CDS sequence) NM_001330075.2 , NM_001346248.2 , NM_001346248.1 , NM_001330075.3
    Accession Version NM_001330075.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Jun 2, 2019 this sequence version replaced NM_001330075.2. Transcript Variant: This variant (5) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (5) is longer than isoform 1. Variants 5 and 10 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.131652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317004.1
    CDS461..7948
    Translation

    Target ORF information:

    RefSeq Version NM_001330075.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330075.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84852
    Clone ID Related Accession (Same CDS sequence) XM_017021143.1 , XM_017021143.2
    Accession Version XM_017021143.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTG GGGAAATTCT GTTATTATTG CTTCAAATAT TGCTTCTGTT
    CCTTTTTCTC TTTCTTCTTC TTTTGGAGTT CCCATTACAT GTATTTACAT CTTTCATGGT
    AGTCCCACAG TTTTTGCATA TTCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876632.1
    CDS470..7819
    Translation

    Target ORF information:

    RefSeq Version XM_017021143.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021143.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTG GGGAAATTCT GTTATTATTG CTTCAAATAT TGCTTCTGTT
    CCTTTTTCTC TTTCTTCTTC TTTTGGAGTT CCCATTACAT GTATTTACAT CTTTCATGGT
    AGTCCCACAG TTTTTGCATA TTCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84852
    Clone ID Related Accession (Same CDS sequence) XM_017021143.1 , XM_017021143.2
    Accession Version XM_017021143.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTG GGGAAATTCT GTTATTATTG CTTCAAATAT TGCTTCTGTT
    CCTTTTTCTC TTTCTTCTTC TTTTGGAGTT CCCATTACAT GTATTTACAT CTTTCATGGT
    AGTCCCACAG TTTTTGCATA TTCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876632.1
    CDS176..7525
    Translation

    Target ORF information:

    RefSeq Version XM_017021143.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021143.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTG GGGAAATTCT GTTATTATTG CTTCAAATAT TGCTTCTGTT
    CCTTTTTCTC TTTCTTCTTC TTTTGGAGTT CCCATTACAT GTATTTACAT CTTTCATGGT
    AGTCCCACAG TTTTTGCATA TTCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02421
    Clone ID Related Accession (Same CDS sequence) NM_194301.3 , NM_194301.4
    Accession Version NM_194301.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5 and AY596971.1. On Oct 4, 2016 this sequence version replaced NM_194301.2. Transcript Variant: This variant (2) differs in the 3' UTR and lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is longer and has a distinct C-terminus, compared to isoform 1. Isoforms 2 and 4 are the same length, but have distinct protein sequences. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY596971.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGGCTCCTA CCCAGAGATT
    CTACCCAGTG AAACTCCCAC AGCAACGCAG GTAGATGGGG CTGACCTGGC CTCTCCAATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGTTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_919277.2
    CDS489..6740
    Translation

    Target ORF information:

    RefSeq Version NM_194301.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_194301.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGGCTCCTA CCCAGAGATT
    CTACCCAGTG AAACTCCCAC AGCAACGCAG GTAGATGGGG CTGACCTGGC CTCTCCAATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGTTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02421
    Clone ID Related Accession (Same CDS sequence) NM_194301.3 , NM_194301.4
    Accession Version NM_194301.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5 and AY596971.1. On Jun 2, 2019 this sequence version replaced NM_194301.3. Transcript Variant: This variant (2) differs in the 3' UTR and lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is longer and has a distinct C-terminus, compared to isoform 1. Isoforms 2 and 4 are the same length, but have distinct protein sequences. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY596971.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGGCTCCTA CCCAGAGATT
    CTACCCAGTG AAACTCCCAC AGCAACGCAG GTAGATGGGG CTGACCTGGC CTCTCCAATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGTTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_919277.2
    CDS461..6712
    Translation

    Target ORF information:

    RefSeq Version NM_194301.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_194301.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGGCTCCTA CCCAGAGATT
    CTACCCAGTG AAACTCCCAC AGCAACGCAG GTAGATGGGG CTGACCTGGC CTCTCCAATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGTTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu05247
    Clone ID Related Accession (Same CDS sequence) NM_001283043.3 , NM_001283043.2
    Accession Version NM_001283043.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AY596970.1 and BC150596.1. On Jun 2, 2019 this sequence version replaced NM_001283043.2. Transcript Variant: This variant (3) contains an alternate exon and uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC150596.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGAGGCAG AACAGAATGC CACCAGAGGC
    TCCACTGAGG GATCTGTACA GTCATGTAAT GGGCTATTTT GGAAGGAAAG CTGCAGTGAT
    ATTGGCGGCA GCAGTGCTAA TGTTCCTGAT CTGATGGATG AGTTTATAGC AGAACGACTT
    CGAAGTGGTA ATGCCTCGAC TATGACAAGA AGAGGAAGTA GTCCAGGCAG CCTGGAAATT
    CCCAAAGACC TCCCTGATAT TCTAAACAAG CAGAACCAGA TGCGCCCTAT TGATGACCCA
    GGTGTGCCCT CAGAATGGAC TTCTCCTGCC AGTGCAGGGA GCAGTGATCT TATCAGCTCA
    GATAGTCATT CGGATTCTTT CAGCGCTTTC CAATATGATG GCCGAAAATT TGACAATTTT
    GGCTTTGGAA CCGACACTGG GGTTACGTCC TCTGCTGATG TGGATTCAGG TTCTGGCCAT
    CATCAGAGTG CTGAAGAGCA GGAAGTGGCT AGTCTAACTA CTCTTCATAT AGATTCTGAA
    ACAAGCAGTC TTAATCAGCA AGCTTTCTCT GCTGAAGTTG CAACTATTAC TGGTTCAGAA
    AGTGCTTCCC CAGTCCACTC ACCTCTGGGC TCCAGGTCAC AGACTCCTTC CCCTTCTACA
    TTGAATATAG ATCACATGGA ACAGAAGGAT CTGCAGCTCG ACGAGAAGCT CCACCACTCT
    GTTCTTCAGA CACCAGATGA TCTAGAAATT AGTGAATTTC CATCAGAATG TTGTAGTGTG
    ATGGCAGGAG GTACTCTGAC TGGATGGCAT GCTGATGTTG CTACTGTAAT GTGGCGAAGA
    ATGCTAGGCA TTTTGGGAGA TGTAAATTCA ATCATGGATC CTGAAATACA TGCTCAAGTT
    TTTGATTACC TCTGTGAACT TTGGCAGAAT CTAGCTAAGA TTAGAGATAA CCTTGGCATT
    TCAACTGATA ACCTGACCTC CCCTTCTCCA CCAGTTTTAA TTCCTCCACT GAGAATTCTT
    ACACCTTGGC TTTTTAAGGC AACCATGTTG ACTGATAAAT ATAAACAAGG TAAATTACAT
    GCATATAAAC TTATTTGTAA TACAATGAAA AGAAGACAAG ATGTTTCTCC AAATAGAGAT
    TTTCTAACAC ATTTCTACAA TATAATGCAT TGTGGATTAC TTCATATTGA CCAGGATATT
    GTCAATACAA TCATCAAACA CTGCTCACCT CAATTTTTTT CACTTGGTTT GCCTGGTGCC
    ACAATGCTTA TTATGGATTT TATTGTAGCA GCTGGTAGAG TGGCTTCTTC AGCTTTTCTC
    AATGCACCAA GAGTAGAAGC ACAAGTTCTT CTGGGATCTT TGGTTTGCTT TCCCAACTTA
    TATTGTGAAC TGCCTTCTCT TCATCCCAAC ATTCCTGATG TTGCTGTGTC TCAGTTTACA
    GATGTTAAGG AACTTATAAT CAAAACTGTA TTAAGCTCGG CAAGAGATGA GCCCTCTGGT
    CCTGCACGAT GTGTAGCACT TTGTAGTTTA GGTATTTGGA TTTGTGAAGA ACTAGTCCAT
    GAGTCTCATC ATCCTCAAAT TAAGGAAGCT CTGAATGTGA TTTGTGTTTC CTTAAAGTTT
    ACTAATAAAA CAGTAGCCCA CGTAGCTTGT AACATGCTTC ACATGCTGGT TCATTATGTA
    CCTAGACTTC AGATTTACCA GCCTGATTCT CCCTTGAAAA TTATTCAAAT CCTAATAGCT
    ACCATCACCC ATCTTTTACC AAGTACAGAG GCTTCATCTT ATGAAATGGA CAAGAGGTTG
    GTAGTATCTT TACTTCTCTG CCTTCTGGAC TGGATCATGG CCTTACCTCT AAAGACACTG
    CTCCAACCAT TTCATGCTAC GGGAGCAGAA AGCGATAAAA CAGAAAAATC TGTTCTCAAT
    TGCATTTATA AGGTTTTACA TGGGTGTGTT TATGGAGCTC AGTGTTTTAG CAATCCAAGG
    TATTTTCCCA TGAGCCTCTC TGATTTGGCA TCTGTAGATT ATGATCCTTT TATGCATTTG
    GAAAGTCTGA AAGAGCCTGA GCCTCTGCAC TCTCCTGACT CAGAACGATC TTCTAAACTC
    CAGCCAGTAA CAGAAGTGAA AACTCAAATG CAGCATGGAT TAATCTCTAT AGCAGCCCGC
    ACTGTTATTA CACATCTGGT AAATCACCTG GGCCATTATC CAATGAGCGG TGGTCCTGCT
    ATGCTAACAA GTCAGGTGTG TGAAAATCAC GACAATCATT ACAGTGAAAG TACTGAACTT
    TCTCCTGAAC TCTTTGAGAG TCCAAATATC CAGTTCTTTG TGTTAAATAA TACAACCTTA
    GTGTCCTGTA TCCAGATCAG ATCAGAAGAG AATATGCCTG GAGGAGGTTT ATCTGCTGGC
    CTTGCATCAG CCAATTCAAA TGTCAGAATC ATAGTACGTG ATCTCTCTGG AAAATATTCA
    TGGGATTCTG CTATACTGTA TGGCCCACCT CCTGTAAGTG GCTTGTCAGA ACCTACATCT
    TTCATGCTTT CATTGTCTCA CCAAGAGAAG CCAGAAGAGC CTCCGACATC TAATGAATGC
    TTAGAAGATA TAACCGTAAA AGATGGACTT TCTCTCCAGT TTAAAAGATT TAGAGAAACT
    GTACCAACTT GGGATACAAT AAGAGATGAA GAAGATGTTC TTGATGAGCT CTTGCAGTAT
    TTGGGTGTTA CTAGTCCTGA ATGCTTACAG AGAACTGGAA TCTCACTTAA TATTCCTGCT
    CCACAACCTG TGTGCATTTC TGAAAAACAA GAAAATGATG TTATTAATGC TATCCTTAAG
    CAACATACAG AAGAAAAAGA ATTTGTTGAG AAGCACTTTA ATGACTTAAA CATGAAAGCT
    GTGGAACAAG ATGAACCAAT ACCTCAAAAA CCTCAGTCAG CATTTTATTA TTGCAGATTG
    CTTCTTAGTA TATTGGGAAT GAATTCCTGG GACAAACGGA GGAGCTTTCA TCTCCTGAAG
    AAAAATGAAA AGCTACTTAG AGAACTTAGG AACTTGGATT CAAGGCAGTG CCGAGAGACA
    CACAAGATTG CAGTATTTTA TGTTGCTGAA GGACAAGAAG ACAAACACTC CATTCTCACC
    AATACAGGAG GAAGTCAAGC ATATGAAGAT TTTGTAGCTG GTCTTGGTTG GGAGGTAAAT
    CTTACAAACC ATTGTGGTTT TATGGGAGGA CTACAAAAAA ACAAAAGCAC TGGATTGACC
    ACTCCATATT TTGCTACCTC TACAGTAGAG GTAATATTTC ACGTGTCAAC AAGAATGCCT
    TCTGATTCTG ATGATTCTTT GACCAAAAAA TTGAGACATT TGGGAAATGA TGAAGTGCAC
    ATTGTTTGGT CAGAGCATAC TAGAGACTAC AGGAGAGGAA TTATTCCCAC AGAATTTGGT
    GATGTCCTTA TTGTAATATA TCCAATGAAA AATCACATGT TCAGTATTCA GATAATGAAA
    AAACCAGAGG TTCCCTTCTT TGGTCCCCTT TTTGATGGTG CTATTGTGAA TGGAAAGGTT
    CTACCCATTA TGGTTAGAGC AACAGCTATA AATGCAAGCC GTGCTCTGAA ATCTCTGATT
    CCATTGTATC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTCCAGCAC
    CACTTAGAAC CAACAACATT TGAAGATTTT GCAGCACAGG TTTTTTCTCC AGCTCCCTAC
    CACCATTTAC CATCTGATGC CGATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269972.1
    CDS461..6610
    Translation

    Target ORF information:

    RefSeq Version NM_001283043.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001283043.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGAGGCAG AACAGAATGC CACCAGAGGC
    TCCACTGAGG GATCTGTACA GTCATGTAAT GGGCTATTTT GGAAGGAAAG CTGCAGTGAT
    ATTGGCGGCA GCAGTGCTAA TGTTCCTGAT CTGATGGATG AGTTTATAGC AGAACGACTT
    CGAAGTGGTA ATGCCTCGAC TATGACAAGA AGAGGAAGTA GTCCAGGCAG CCTGGAAATT
    CCCAAAGACC TCCCTGATAT TCTAAACAAG CAGAACCAGA TGCGCCCTAT TGATGACCCA
    GGTGTGCCCT CAGAATGGAC TTCTCCTGCC AGTGCAGGGA GCAGTGATCT TATCAGCTCA
    GATAGTCATT CGGATTCTTT CAGCGCTTTC CAATATGATG GCCGAAAATT TGACAATTTT
    GGCTTTGGAA CCGACACTGG GGTTACGTCC TCTGCTGATG TGGATTCAGG TTCTGGCCAT
    CATCAGAGTG CTGAAGAGCA GGAAGTGGCT AGTCTAACTA CTCTTCATAT AGATTCTGAA
    ACAAGCAGTC TTAATCAGCA AGCTTTCTCT GCTGAAGTTG CAACTATTAC TGGTTCAGAA
    AGTGCTTCCC CAGTCCACTC ACCTCTGGGC TCCAGGTCAC AGACTCCTTC CCCTTCTACA
    TTGAATATAG ATCACATGGA ACAGAAGGAT CTGCAGCTCG ACGAGAAGCT CCACCACTCT
    GTTCTTCAGA CACCAGATGA TCTAGAAATT AGTGAATTTC CATCAGAATG TTGTAGTGTG
    ATGGCAGGAG GTACTCTGAC TGGATGGCAT GCTGATGTTG CTACTGTAAT GTGGCGAAGA
    ATGCTAGGCA TTTTGGGAGA TGTAAATTCA ATCATGGATC CTGAAATACA TGCTCAAGTT
    TTTGATTACC TCTGTGAACT TTGGCAGAAT CTAGCTAAGA TTAGAGATAA CCTTGGCATT
    TCAACTGATA ACCTGACCTC CCCTTCTCCA CCAGTTTTAA TTCCTCCACT GAGAATTCTT
    ACACCTTGGC TTTTTAAGGC AACCATGTTG ACTGATAAAT ATAAACAAGG TAAATTACAT
    GCATATAAAC TTATTTGTAA TACAATGAAA AGAAGACAAG ATGTTTCTCC AAATAGAGAT
    TTTCTAACAC ATTTCTACAA TATAATGCAT TGTGGATTAC TTCATATTGA CCAGGATATT
    GTCAATACAA TCATCAAACA CTGCTCACCT CAATTTTTTT CACTTGGTTT GCCTGGTGCC
    ACAATGCTTA TTATGGATTT TATTGTAGCA GCTGGTAGAG TGGCTTCTTC AGCTTTTCTC
    AATGCACCAA GAGTAGAAGC ACAAGTTCTT CTGGGATCTT TGGTTTGCTT TCCCAACTTA
    TATTGTGAAC TGCCTTCTCT TCATCCCAAC ATTCCTGATG TTGCTGTGTC TCAGTTTACA
    GATGTTAAGG AACTTATAAT CAAAACTGTA TTAAGCTCGG CAAGAGATGA GCCCTCTGGT
    CCTGCACGAT GTGTAGCACT TTGTAGTTTA GGTATTTGGA TTTGTGAAGA ACTAGTCCAT
    GAGTCTCATC ATCCTCAAAT TAAGGAAGCT CTGAATGTGA TTTGTGTTTC CTTAAAGTTT
    ACTAATAAAA CAGTAGCCCA CGTAGCTTGT AACATGCTTC ACATGCTGGT TCATTATGTA
    CCTAGACTTC AGATTTACCA GCCTGATTCT CCCTTGAAAA TTATTCAAAT CCTAATAGCT
    ACCATCACCC ATCTTTTACC AAGTACAGAG GCTTCATCTT ATGAAATGGA CAAGAGGTTG
    GTAGTATCTT TACTTCTCTG CCTTCTGGAC TGGATCATGG CCTTACCTCT AAAGACACTG
    CTCCAACCAT TTCATGCTAC GGGAGCAGAA AGCGATAAAA CAGAAAAATC TGTTCTCAAT
    TGCATTTATA AGGTTTTACA TGGGTGTGTT TATGGAGCTC AGTGTTTTAG CAATCCAAGG
    TATTTTCCCA TGAGCCTCTC TGATTTGGCA TCTGTAGATT ATGATCCTTT TATGCATTTG
    GAAAGTCTGA AAGAGCCTGA GCCTCTGCAC TCTCCTGACT CAGAACGATC TTCTAAACTC
    CAGCCAGTAA CAGAAGTGAA AACTCAAATG CAGCATGGAT TAATCTCTAT AGCAGCCCGC
    ACTGTTATTA CACATCTGGT AAATCACCTG GGCCATTATC CAATGAGCGG TGGTCCTGCT
    ATGCTAACAA GTCAGGTGTG TGAAAATCAC GACAATCATT ACAGTGAAAG TACTGAACTT
    TCTCCTGAAC TCTTTGAGAG TCCAAATATC CAGTTCTTTG TGTTAAATAA TACAACCTTA
    GTGTCCTGTA TCCAGATCAG ATCAGAAGAG AATATGCCTG GAGGAGGTTT ATCTGCTGGC
    CTTGCATCAG CCAATTCAAA TGTCAGAATC ATAGTACGTG ATCTCTCTGG AAAATATTCA
    TGGGATTCTG CTATACTGTA TGGCCCACCT CCTGTAAGTG GCTTGTCAGA ACCTACATCT
    TTCATGCTTT CATTGTCTCA CCAAGAGAAG CCAGAAGAGC CTCCGACATC TAATGAATGC
    TTAGAAGATA TAACCGTAAA AGATGGACTT TCTCTCCAGT TTAAAAGATT TAGAGAAACT
    GTACCAACTT GGGATACAAT AAGAGATGAA GAAGATGTTC TTGATGAGCT CTTGCAGTAT
    TTGGGTGTTA CTAGTCCTGA ATGCTTACAG AGAACTGGAA TCTCACTTAA TATTCCTGCT
    CCACAACCTG TGTGCATTTC TGAAAAACAA GAAAATGATG TTATTAATGC TATCCTTAAG
    CAACATACAG AAGAAAAAGA ATTTGTTGAG AAGCACTTTA ATGACTTAAA CATGAAAGCT
    GTGGAACAAG ATGAACCAAT ACCTCAAAAA CCTCAGTCAG CATTTTATTA TTGCAGATTG
    CTTCTTAGTA TATTGGGAAT GAATTCCTGG GACAAACGGA GGAGCTTTCA TCTCCTGAAG
    AAAAATGAAA AGCTACTTAG AGAACTTAGG AACTTGGATT CAAGGCAGTG CCGAGAGACA
    CACAAGATTG CAGTATTTTA TGTTGCTGAA GGACAAGAAG ACAAACACTC CATTCTCACC
    AATACAGGAG GAAGTCAAGC ATATGAAGAT TTTGTAGCTG GTCTTGGTTG GGAGGTAAAT
    CTTACAAACC ATTGTGGTTT TATGGGAGGA CTACAAAAAA ACAAAAGCAC TGGATTGACC
    ACTCCATATT TTGCTACCTC TACAGTAGAG GTAATATTTC ACGTGTCAAC AAGAATGCCT
    TCTGATTCTG ATGATTCTTT GACCAAAAAA TTGAGACATT TGGGAAATGA TGAAGTGCAC
    ATTGTTTGGT CAGAGCATAC TAGAGACTAC AGGAGAGGAA TTATTCCCAC AGAATTTGGT
    GATGTCCTTA TTGTAATATA TCCAATGAAA AATCACATGT TCAGTATTCA GATAATGAAA
    AAACCAGAGG TTCCCTTCTT TGGTCCCCTT TTTGATGGTG CTATTGTGAA TGGAAAGGTT
    CTACCCATTA TGGTTAGAGC AACAGCTATA AATGCAAGCC GTGCTCTGAA ATCTCTGATT
    CCATTGTATC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTCCAGCAC
    CACTTAGAAC CAACAACATT TGAAGATTTT GCAGCACAGG TTTTTTCTCC AGCTCCCTAC
    CACCATTTAC CATCTGATGC CGATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu05247
    Clone ID Related Accession (Same CDS sequence) NM_001283043.3 , NM_001283043.2
    Accession Version NM_001283043.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AY596970.1 and BC150596.1. On Oct 4, 2016 this sequence version replaced NM_001283043.1. Transcript Variant: This variant (3) contains an alternate exon and uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC150596.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGAGGCAG AACAGAATGC CACCAGAGGC
    TCCACTGAGG GATCTGTACA GTCATGTAAT GGGCTATTTT GGAAGGAAAG CTGCAGTGAT
    ATTGGCGGCA GCAGTGCTAA TGTTCCTGAT CTGATGGATG AGTTTATAGC AGAACGACTT
    CGAAGTGGTA ATGCCTCGAC TATGACAAGA AGAGGAAGTA GTCCAGGCAG CCTGGAAATT
    CCCAAAGACC TCCCTGATAT TCTAAACAAG CAGAACCAGA TGCGCCCTAT TGATGACCCA
    GGTGTGCCCT CAGAATGGAC TTCTCCTGCC AGTGCAGGGA GCAGTGATCT TATCAGCTCA
    GATAGTCATT CGGATTCTTT CAGCGCTTTC CAATATGATG GCCGAAAATT TGACAATTTT
    GGCTTTGGAA CCGACACTGG GGTTACGTCC TCTGCTGATG TGGATTCAGG TTCTGGCCAT
    CATCAGAGTG CTGAAGAGCA GGAAGTGGCT AGTCTAACTA CTCTTCATAT AGATTCTGAA
    ACAAGCAGTC TTAATCAGCA AGCTTTCTCT GCTGAAGTTG CAACTATTAC TGGTTCAGAA
    AGTGCTTCCC CAGTCCACTC ACCTCTGGGC TCCAGGTCAC AGACTCCTTC CCCTTCTACA
    TTGAATATAG ATCACATGGA ACAGAAGGAT CTGCAGCTCG ACGAGAAGCT CCACCACTCT
    GTTCTTCAGA CACCAGATGA TCTAGAAATT AGTGAATTTC CATCAGAATG TTGTAGTGTG
    ATGGCAGGAG GTACTCTGAC TGGATGGCAT GCTGATGTTG CTACTGTAAT GTGGCGAAGA
    ATGCTAGGCA TTTTGGGAGA TGTAAATTCA ATCATGGATC CTGAAATACA TGCTCAAGTT
    TTTGATTACC TCTGTGAACT TTGGCAGAAT CTAGCTAAGA TTAGAGATAA CCTTGGCATT
    TCAACTGATA ACCTGACCTC CCCTTCTCCA CCAGTTTTAA TTCCTCCACT GAGAATTCTT
    ACACCTTGGC TTTTTAAGGC AACCATGTTG ACTGATAAAT ATAAACAAGG TAAATTACAT
    GCATATAAAC TTATTTGTAA TACAATGAAA AGAAGACAAG ATGTTTCTCC AAATAGAGAT
    TTTCTAACAC ATTTCTACAA TATAATGCAT TGTGGATTAC TTCATATTGA CCAGGATATT
    GTCAATACAA TCATCAAACA CTGCTCACCT CAATTTTTTT CACTTGGTTT GCCTGGTGCC
    ACAATGCTTA TTATGGATTT TATTGTAGCA GCTGGTAGAG TGGCTTCTTC AGCTTTTCTC
    AATGCACCAA GAGTAGAAGC ACAAGTTCTT CTGGGATCTT TGGTTTGCTT TCCCAACTTA
    TATTGTGAAC TGCCTTCTCT TCATCCCAAC ATTCCTGATG TTGCTGTGTC TCAGTTTACA
    GATGTTAAGG AACTTATAAT CAAAACTGTA TTAAGCTCGG CAAGAGATGA GCCCTCTGGT
    CCTGCACGAT GTGTAGCACT TTGTAGTTTA GGTATTTGGA TTTGTGAAGA ACTAGTCCAT
    GAGTCTCATC ATCCTCAAAT TAAGGAAGCT CTGAATGTGA TTTGTGTTTC CTTAAAGTTT
    ACTAATAAAA CAGTAGCCCA CGTAGCTTGT AACATGCTTC ACATGCTGGT TCATTATGTA
    CCTAGACTTC AGATTTACCA GCCTGATTCT CCCTTGAAAA TTATTCAAAT CCTAATAGCT
    ACCATCACCC ATCTTTTACC AAGTACAGAG GCTTCATCTT ATGAAATGGA CAAGAGGTTG
    GTAGTATCTT TACTTCTCTG CCTTCTGGAC TGGATCATGG CCTTACCTCT AAAGACACTG
    CTCCAACCAT TTCATGCTAC GGGAGCAGAA AGCGATAAAA CAGAAAAATC TGTTCTCAAT
    TGCATTTATA AGGTTTTACA TGGGTGTGTT TATGGAGCTC AGTGTTTTAG CAATCCAAGG
    TATTTTCCCA TGAGCCTCTC TGATTTGGCA TCTGTAGATT ATGATCCTTT TATGCATTTG
    GAAAGTCTGA AAGAGCCTGA GCCTCTGCAC TCTCCTGACT CAGAACGATC TTCTAAACTC
    CAGCCAGTAA CAGAAGTGAA AACTCAAATG CAGCATGGAT TAATCTCTAT AGCAGCCCGC
    ACTGTTATTA CACATCTGGT AAATCACCTG GGCCATTATC CAATGAGCGG TGGTCCTGCT
    ATGCTAACAA GTCAGGTGTG TGAAAATCAC GACAATCATT ACAGTGAAAG TACTGAACTT
    TCTCCTGAAC TCTTTGAGAG TCCAAATATC CAGTTCTTTG TGTTAAATAA TACAACCTTA
    GTGTCCTGTA TCCAGATCAG ATCAGAAGAG AATATGCCTG GAGGAGGTTT ATCTGCTGGC
    CTTGCATCAG CCAATTCAAA TGTCAGAATC ATAGTACGTG ATCTCTCTGG AAAATATTCA
    TGGGATTCTG CTATACTGTA TGGCCCACCT CCTGTAAGTG GCTTGTCAGA ACCTACATCT
    TTCATGCTTT CATTGTCTCA CCAAGAGAAG CCAGAAGAGC CTCCGACATC TAATGAATGC
    TTAGAAGATA TAACCGTAAA AGATGGACTT TCTCTCCAGT TTAAAAGATT TAGAGAAACT
    GTACCAACTT GGGATACAAT AAGAGATGAA GAAGATGTTC TTGATGAGCT CTTGCAGTAT
    TTGGGTGTTA CTAGTCCTGA ATGCTTACAG AGAACTGGAA TCTCACTTAA TATTCCTGCT
    CCACAACCTG TGTGCATTTC TGAAAAACAA GAAAATGATG TTATTAATGC TATCCTTAAG
    CAACATACAG AAGAAAAAGA ATTTGTTGAG AAGCACTTTA ATGACTTAAA CATGAAAGCT
    GTGGAACAAG ATGAACCAAT ACCTCAAAAA CCTCAGTCAG CATTTTATTA TTGCAGATTG
    CTTCTTAGTA TATTGGGAAT GAATTCCTGG GACAAACGGA GGAGCTTTCA TCTCCTGAAG
    AAAAATGAAA AGCTACTTAG AGAACTTAGG AACTTGGATT CAAGGCAGTG CCGAGAGACA
    CACAAGATTG CAGTATTTTA TGTTGCTGAA GGACAAGAAG ACAAACACTC CATTCTCACC
    AATACAGGAG GAAGTCAAGC ATATGAAGAT TTTGTAGCTG GTCTTGGTTG GGAGGTAAAT
    CTTACAAACC ATTGTGGTTT TATGGGAGGA CTACAAAAAA ACAAAAGCAC TGGATTGACC
    ACTCCATATT TTGCTACCTC TACAGTAGAG GTAATATTTC ACGTGTCAAC AAGAATGCCT
    TCTGATTCTG ATGATTCTTT GACCAAAAAA TTGAGACATT TGGGAAATGA TGAAGTGCAC
    ATTGTTTGGT CAGAGCATAC TAGAGACTAC AGGAGAGGAA TTATTCCCAC AGAATTTGGT
    GATGTCCTTA TTGTAATATA TCCAATGAAA AATCACATGT TCAGTATTCA GATAATGAAA
    AAACCAGAGG TTCCCTTCTT TGGTCCCCTT TTTGATGGTG CTATTGTGAA TGGAAAGGTT
    CTACCCATTA TGGTTAGAGC AACAGCTATA AATGCAAGCC GTGCTCTGAA ATCTCTGATT
    CCATTGTATC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTCCAGCAC
    CACTTAGAAC CAACAACATT TGAAGATTTT GCAGCACAGG TTTTTTCTCC AGCTCCCTAC
    CACCATTTAC CATCTGATGC CGATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269972.1
    CDS489..6638
    Translation

    Target ORF information:

    RefSeq Version NM_001283043.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001283043.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGAGGCAG AACAGAATGC CACCAGAGGC
    TCCACTGAGG GATCTGTACA GTCATGTAAT GGGCTATTTT GGAAGGAAAG CTGCAGTGAT
    ATTGGCGGCA GCAGTGCTAA TGTTCCTGAT CTGATGGATG AGTTTATAGC AGAACGACTT
    CGAAGTGGTA ATGCCTCGAC TATGACAAGA AGAGGAAGTA GTCCAGGCAG CCTGGAAATT
    CCCAAAGACC TCCCTGATAT TCTAAACAAG CAGAACCAGA TGCGCCCTAT TGATGACCCA
    GGTGTGCCCT CAGAATGGAC TTCTCCTGCC AGTGCAGGGA GCAGTGATCT TATCAGCTCA
    GATAGTCATT CGGATTCTTT CAGCGCTTTC CAATATGATG GCCGAAAATT TGACAATTTT
    GGCTTTGGAA CCGACACTGG GGTTACGTCC TCTGCTGATG TGGATTCAGG TTCTGGCCAT
    CATCAGAGTG CTGAAGAGCA GGAAGTGGCT AGTCTAACTA CTCTTCATAT AGATTCTGAA
    ACAAGCAGTC TTAATCAGCA AGCTTTCTCT GCTGAAGTTG CAACTATTAC TGGTTCAGAA
    AGTGCTTCCC CAGTCCACTC ACCTCTGGGC TCCAGGTCAC AGACTCCTTC CCCTTCTACA
    TTGAATATAG ATCACATGGA ACAGAAGGAT CTGCAGCTCG ACGAGAAGCT CCACCACTCT
    GTTCTTCAGA CACCAGATGA TCTAGAAATT AGTGAATTTC CATCAGAATG TTGTAGTGTG
    ATGGCAGGAG GTACTCTGAC TGGATGGCAT GCTGATGTTG CTACTGTAAT GTGGCGAAGA
    ATGCTAGGCA TTTTGGGAGA TGTAAATTCA ATCATGGATC CTGAAATACA TGCTCAAGTT
    TTTGATTACC TCTGTGAACT TTGGCAGAAT CTAGCTAAGA TTAGAGATAA CCTTGGCATT
    TCAACTGATA ACCTGACCTC CCCTTCTCCA CCAGTTTTAA TTCCTCCACT GAGAATTCTT
    ACACCTTGGC TTTTTAAGGC AACCATGTTG ACTGATAAAT ATAAACAAGG TAAATTACAT
    GCATATAAAC TTATTTGTAA TACAATGAAA AGAAGACAAG ATGTTTCTCC AAATAGAGAT
    TTTCTAACAC ATTTCTACAA TATAATGCAT TGTGGATTAC TTCATATTGA CCAGGATATT
    GTCAATACAA TCATCAAACA CTGCTCACCT CAATTTTTTT CACTTGGTTT GCCTGGTGCC
    ACAATGCTTA TTATGGATTT TATTGTAGCA GCTGGTAGAG TGGCTTCTTC AGCTTTTCTC
    AATGCACCAA GAGTAGAAGC ACAAGTTCTT CTGGGATCTT TGGTTTGCTT TCCCAACTTA
    TATTGTGAAC TGCCTTCTCT TCATCCCAAC ATTCCTGATG TTGCTGTGTC TCAGTTTACA
    GATGTTAAGG AACTTATAAT CAAAACTGTA TTAAGCTCGG CAAGAGATGA GCCCTCTGGT
    CCTGCACGAT GTGTAGCACT TTGTAGTTTA GGTATTTGGA TTTGTGAAGA ACTAGTCCAT
    GAGTCTCATC ATCCTCAAAT TAAGGAAGCT CTGAATGTGA TTTGTGTTTC CTTAAAGTTT
    ACTAATAAAA CAGTAGCCCA CGTAGCTTGT AACATGCTTC ACATGCTGGT TCATTATGTA
    CCTAGACTTC AGATTTACCA GCCTGATTCT CCCTTGAAAA TTATTCAAAT CCTAATAGCT
    ACCATCACCC ATCTTTTACC AAGTACAGAG GCTTCATCTT ATGAAATGGA CAAGAGGTTG
    GTAGTATCTT TACTTCTCTG CCTTCTGGAC TGGATCATGG CCTTACCTCT AAAGACACTG
    CTCCAACCAT TTCATGCTAC GGGAGCAGAA AGCGATAAAA CAGAAAAATC TGTTCTCAAT
    TGCATTTATA AGGTTTTACA TGGGTGTGTT TATGGAGCTC AGTGTTTTAG CAATCCAAGG
    TATTTTCCCA TGAGCCTCTC TGATTTGGCA TCTGTAGATT ATGATCCTTT TATGCATTTG
    GAAAGTCTGA AAGAGCCTGA GCCTCTGCAC TCTCCTGACT CAGAACGATC TTCTAAACTC
    CAGCCAGTAA CAGAAGTGAA AACTCAAATG CAGCATGGAT TAATCTCTAT AGCAGCCCGC
    ACTGTTATTA CACATCTGGT AAATCACCTG GGCCATTATC CAATGAGCGG TGGTCCTGCT
    ATGCTAACAA GTCAGGTGTG TGAAAATCAC GACAATCATT ACAGTGAAAG TACTGAACTT
    TCTCCTGAAC TCTTTGAGAG TCCAAATATC CAGTTCTTTG TGTTAAATAA TACAACCTTA
    GTGTCCTGTA TCCAGATCAG ATCAGAAGAG AATATGCCTG GAGGAGGTTT ATCTGCTGGC
    CTTGCATCAG CCAATTCAAA TGTCAGAATC ATAGTACGTG ATCTCTCTGG AAAATATTCA
    TGGGATTCTG CTATACTGTA TGGCCCACCT CCTGTAAGTG GCTTGTCAGA ACCTACATCT
    TTCATGCTTT CATTGTCTCA CCAAGAGAAG CCAGAAGAGC CTCCGACATC TAATGAATGC
    TTAGAAGATA TAACCGTAAA AGATGGACTT TCTCTCCAGT TTAAAAGATT TAGAGAAACT
    GTACCAACTT GGGATACAAT AAGAGATGAA GAAGATGTTC TTGATGAGCT CTTGCAGTAT
    TTGGGTGTTA CTAGTCCTGA ATGCTTACAG AGAACTGGAA TCTCACTTAA TATTCCTGCT
    CCACAACCTG TGTGCATTTC TGAAAAACAA GAAAATGATG TTATTAATGC TATCCTTAAG
    CAACATACAG AAGAAAAAGA ATTTGTTGAG AAGCACTTTA ATGACTTAAA CATGAAAGCT
    GTGGAACAAG ATGAACCAAT ACCTCAAAAA CCTCAGTCAG CATTTTATTA TTGCAGATTG
    CTTCTTAGTA TATTGGGAAT GAATTCCTGG GACAAACGGA GGAGCTTTCA TCTCCTGAAG
    AAAAATGAAA AGCTACTTAG AGAACTTAGG AACTTGGATT CAAGGCAGTG CCGAGAGACA
    CACAAGATTG CAGTATTTTA TGTTGCTGAA GGACAAGAAG ACAAACACTC CATTCTCACC
    AATACAGGAG GAAGTCAAGC ATATGAAGAT TTTGTAGCTG GTCTTGGTTG GGAGGTAAAT
    CTTACAAACC ATTGTGGTTT TATGGGAGGA CTACAAAAAA ACAAAAGCAC TGGATTGACC
    ACTCCATATT TTGCTACCTC TACAGTAGAG GTAATATTTC ACGTGTCAAC AAGAATGCCT
    TCTGATTCTG ATGATTCTTT GACCAAAAAA TTGAGACATT TGGGAAATGA TGAAGTGCAC
    ATTGTTTGGT CAGAGCATAC TAGAGACTAC AGGAGAGGAA TTATTCCCAC AGAATTTGGT
    GATGTCCTTA TTGTAATATA TCCAATGAAA AATCACATGT TCAGTATTCA GATAATGAAA
    AAACCAGAGG TTCCCTTCTT TGGTCCCCTT TTTGATGGTG CTATTGTGAA TGGAAAGGTT
    CTACCCATTA TGGTTAGAGC AACAGCTATA AATGCAAGCC GTGCTCTGAA ATCTCTGATT
    CCATTGTATC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTCCAGCAC
    CACTTAGAAC CAACAACATT TGAAGATTTT GCAGCACAGG TTTTTTCTCC AGCTCCCTAC
    CACCATTTAC CATCTGATGC CGATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06762
    Clone ID Related Accession (Same CDS sequence) NM_014990.2 , NM_001346243.1 , NM_001346243.2 , NM_014990.3
    Accession Version NM_014990.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5 and AY596970.1. This sequence is a reference standard in the RefSeqGene project. On Oct 4, 2016 this sequence version replaced NM_014990.1. Transcript Variant: This variant (1) encodes the shortest isoform (1). Variants 1 and 6 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY596970.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGATCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055805.1
    CDS489..6599
    Translation

    Target ORF information:

    RefSeq Version NM_014990.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014990.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGATCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06762
    Clone ID Related Accession (Same CDS sequence) NM_014990.2 , NM_001346243.1 , NM_001346243.2 , NM_014990.3
    Accession Version NM_001346243.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Oct 4, 2016 this sequence version replaced XM_017021145.1. Transcript Variant: This variant (6) differs in the 3' UTR compared to variant 1. Variants 1 and 6 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.17756.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGATCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333172.1
    CDS489..6599
    Translation

    Target ORF information:

    RefSeq Version NM_001346243.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGATCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06762
    Clone ID Related Accession (Same CDS sequence) NM_014990.2 , NM_001346243.1 , NM_001346243.2 , NM_014990.3
    Accession Version NM_001346243.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Jun 3, 2019 this sequence version replaced NM_001346243.1. Transcript Variant: This variant (6) differs in the 3' UTR compared to variant 1. Variants 1 and 6 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.17756.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGATCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333172.1
    CDS461..6571
    Translation

    Target ORF information:

    RefSeq Version NM_001346243.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346243.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGATCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06762
    Clone ID Related Accession (Same CDS sequence) NM_014990.2 , NM_001346243.1 , NM_001346243.2 , NM_014990.3
    Accession Version NM_014990.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5 and AY596970.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_014990.2. Transcript Variant: This variant (1) encodes the shortest isoform (1). Variants 1 and 6 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY596970.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGATCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055805.1
    CDS461..6571
    Translation

    Target ORF information:

    RefSeq Version NM_014990.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014990.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGATCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10997
    Clone ID Related Accession (Same CDS sequence) NM_001346245.1 , NM_001283044.3 , NM_001283044.2 , NM_001346245.2
    Accession Version NM_001346245.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-15
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Oct 4, 2016 this sequence version replaced XM_006720103.2. Transcript Variant: This variant (7) differs in the 3' UTR and contains an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is longer than isoform 1. Variants 4 and 7 encode the same isoform (4). Isoforms 2 and 4 are the same length, but have distinct protein sequences. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.131495.1, SRR1660809.255963.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGATCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333174.1
    CDS489..6740
    Translation

    Target ORF information:

    RefSeq Version NM_001346245.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGATCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10997
    Clone ID Related Accession (Same CDS sequence) NM_001346245.1 , NM_001283044.3 , NM_001283044.2 , NM_001346245.2
    Accession Version NM_001283044.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AY596970.1, AB511280.1, AL137818.4, BC150596.1 and BC042045.1. On Jun 2, 2019 this sequence version replaced NM_001283044.2. Transcript Variant: This variant (4) contains an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is longer than isoform 1. Variants 4 and 7 encode the same isoform (4). Isoforms 2 and 4 are the same length, but have distinct protein sequences. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.10484.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGATCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269973.1
    CDS461..6712
    Translation

    Target ORF information:

    RefSeq Version NM_001283044.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001283044.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGATCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10997
    Clone ID Related Accession (Same CDS sequence) NM_001346245.1 , NM_001283044.3 , NM_001283044.2 , NM_001346245.2
    Accession Version NM_001283044.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AY596970.1, AB511280.1, AL137818.4, BC150596.1 and BC042045.1. On or before Oct 4, 2016 this sequence version replaced XM_017021144.1, NM_001283044.1. Transcript Variant: This variant (4) contains an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is longer than isoform 1. Variants 4 and 7 encode the same isoform (4). Isoforms 2 and 4 are the same length, but have distinct protein sequences. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.10484.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGATCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269973.1
    CDS489..6740
    Translation

    Target ORF information:

    RefSeq Version NM_001283044.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001283044.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGATCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10997
    Clone ID Related Accession (Same CDS sequence) NM_001346245.1 , NM_001283044.3 , NM_001283044.2 , NM_001346245.2
    Accession Version NM_001346245.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Jun 2, 2019 this sequence version replaced NM_001346245.1. Transcript Variant: This variant (7) differs in the 3' UTR and contains an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is longer than isoform 1. Variants 4 and 7 encode the same isoform (4). Isoforms 2 and 4 are the same length, but have distinct protein sequences. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.131495.1, SRR1660805.227999.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGATCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333174.1
    CDS461..6712
    Translation

    Target ORF information:

    RefSeq Version NM_001346245.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346245.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGATCATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47632
    Clone ID Related Accession (Same CDS sequence) NM_001346249.1 , XM_006720101.2 , NM_001346249.2 , XM_006720101.3 , XM_011536615.3 , XM_011536615.2
    Accession Version NM_001346249.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Oct 4, 2016 this sequence version replaced XM_006720102.3. Transcript Variant: This variant (11) contains an alternate exon in the coding region, and uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (8) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.51179.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333178.1
    CDS489..8117
    Translation

    Target ORF information:

    RefSeq Version NM_001346249.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47632
    Clone ID Related Accession (Same CDS sequence) NM_001346249.1 , XM_006720101.2 , NM_001346249.2 , XM_006720101.3 , XM_011536615.3 , XM_011536615.2
    Accession Version NM_001346249.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Jun 2, 2019 this sequence version replaced NM_001346249.1. Transcript Variant: This variant (11) contains an alternate exon in the coding region, and uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (8) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.51179.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333178.1
    CDS461..8089
    Translation

    Target ORF information:

    RefSeq Version NM_001346249.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346249.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47633
    Clone ID Related Accession (Same CDS sequence) NM_001346247.2 , NM_001346247.1
    Accession Version NM_001346247.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Jun 2, 2019 this sequence version replaced NM_001346247.1. Transcript Variant: This variant (9) has multiple differences compared to variant 1. These differences result in a novel 3' UTR and 3' coding region compared to variant 1. The encoded isoform (7) is longer and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.198839.1, SRR1660809.106676.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGTTGGCT CCTACCCAGA GATTCTACCC AGTGAAACTC CCACAGCAAC GCAGGTAGAT
    GGGGCTGACC TGGCCTCTCC AATGTCTCCT CGAACTAGCA AAAGCCGCAT GTCCATGAAG
    CTGCGTCGTT CCTCTGGCTC AGCCAATAAA TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333176.1
    CDS461..6856
    Translation

    Target ORF information:

    RefSeq Version NM_001346247.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGTTGGCT CCTACCCAGA GATTCTACCC AGTGAAACTC CCACAGCAAC GCAGGTAGAT
    GGGGCTGACC TGGCCTCTCC AATGTCTCCT CGAACTAGCA AAAGCCGCAT GTCCATGAAG
    CTGCGTCGTT CCTCTGGCTC AGCCAATAAA TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47633
    Clone ID Related Accession (Same CDS sequence) NM_001346247.2 , NM_001346247.1
    Accession Version NM_001346247.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Oct 4, 2016 this sequence version replaced XM_005267491.3. Transcript Variant: This variant (9) has multiple differences compared to variant 1. These differences result in a novel 3' UTR and 3' coding region compared to variant 1. The encoded isoform (7) is longer and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.106676.1, SRR1803616.198839.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGTTGGCT CCTACCCAGA GATTCTACCC AGTGAAACTC CCACAGCAAC GCAGGTAGAT
    GGGGCTGACC TGGCCTCTCC AATGTCTCCT CGAACTAGCA AAAGCCGCAT GTCCATGAAG
    CTGCGTCGTT CCTCTGGCTC AGCCAATAAA TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333176.1
    CDS489..6884
    Translation

    Target ORF information:

    RefSeq Version NM_001346247.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGTTGGCT CCTACCCAGA GATTCTACCC AGTGAAACTC CCACAGCAAC GCAGGTAGAT
    GGGGCTGACC TGGCCTCTCC AATGTCTCCT CGAACTAGCA AAAGCCGCAT GTCCATGAAG
    CTGCGTCGTT CCTCTGGCTC AGCCAATAAA TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47635
    Clone ID Related Accession (Same CDS sequence) NM_001346246.2 , NM_001346246.1
    Accession Version NM_001346246.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-26
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Jun 2, 2019 this sequence version replaced NM_001346246.1. Transcript Variant: This variant (8) differs in the 3' structure, which results in a frameshift, compared to variant 1. The encoded isoform (6) is longer and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.103538.1, SRR1803615.140364.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGTTGGCTC CTACCCAGAG
    ATTCTACCCA GTGAAACTCC CACAGCAACG CAGGTAGATG GGGCTGACCT GGCCTCTCCA
    ATGTCTCCTC GAACTAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGTTC CTCTGGCTCA
    GCCAATAAAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333175.1
    CDS461..6715
    Translation

    Target ORF information:

    RefSeq Version NM_001346246.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGTTGGCTC CTACCCAGAG
    ATTCTACCCA GTGAAACTCC CACAGCAACG CAGGTAGATG GGGCTGACCT GGCCTCTCCA
    ATGTCTCCTC GAACTAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGTTC CTCTGGCTCA
    GCCAATAAAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47635
    Clone ID Related Accession (Same CDS sequence) NM_001346246.2 , NM_001346246.1
    Accession Version NM_001346246.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162311.5, AL137818.4, AL160231.4 and AY596970.1. On Oct 4, 2016 this sequence version replaced XM_005267493.3. Transcript Variant: This variant (8) differs in the 3' structure, which results in a frameshift, compared to variant 1. The encoded isoform (6) is longer and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.103538.1, SRR1803615.140364.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGTTGGCTC CTACCCAGAG
    ATTCTACCCA GTGAAACTCC CACAGCAACG CAGGTAGATG GGGCTGACCT GGCCTCTCCA
    ATGTCTCCTC GAACTAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGTTC CTCTGGCTCA
    GCCAATAAAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333175.1
    CDS489..6743
    Translation

    Target ORF information:

    RefSeq Version NM_001346246.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGTCAATA AAGAGGACAT GAGCCAAAAA
    CTGCCTCCTC TTAATAGTGA TATTGGCGGC AGCAGTGCTA ATGTTCCTGA TCTGATGGAT
    GAGTTTATAG CAGAACGACT TCGAAGTGGT AATGCCTCGA CTATGACAAG AAGAGGAAGT
    AGTCCAGGCA GCCTGGAAAT TCCCAAAGAC CTCCCTGATA TTCTAAACAA GCAGAACCAG
    ATGCGCCCTA TTGATGACCC AGGTGTGCCC TCAGAATGGA CTTCTCCTGC CAGTGCAGGG
    AGCAGTGATC TTATCAGCTC AGATAGTCAT TCGGATTCTT TCAGCGCTTT CCAATATGAT
    GGCCGAAAAT TTGACAATTT TGGCTTTGGA ACCGACACTG GGGTTACGTC CTCTGCTGAT
    GTGGATTCAG GTTCTGGCCA TCATCAGAGT GCTGAAGAGC AGGAAGTGGC TAGTCTAACT
    ACTCTTCATA TAGATTCTGA AACAAGCAGT CTTAATCAGC AAGCTTTCTC TGCTGAAGTT
    GCAACTATTA CTGGTTCAGA AAGTGCTTCC CCAGTCCACT CACCTCTGGG CTCCAGGTCA
    CAGACTCCTT CCCCTTCTAC ATTGAATATA GATCACATGG AACAGAAGGA TCTGCAGCTC
    GACGAGAAGC TCCACCACTC TGTTCTTCAG ACACCAGATG ATCTAGAAAT TAGTGAATTT
    CCATCAGAAT GTTGTAGTGT GATGGCAGGA GGTACTCTGA CTGGATGGCA TGCTGATGTT
    GCTACTGTAA TGTGGCGAAG AATGCTAGGC ATTTTGGGAG ATGTAAATTC AATCATGGAT
    CCTGAAATAC ATGCTCAAGT TTTTGATTAC CTCTGTGAAC TTTGGCAGAA TCTAGCTAAG
    ATTAGAGATA ACCTTGGCAT TTCAACTGAT AACCTGACCT CCCCTTCTCC ACCAGTTTTA
    ATTCCTCCAC TGAGAATTCT TACACCTTGG CTTTTTAAGG CAACCATGTT GACTGATAAA
    TATAAACAAG GTAAATTACA TGCATATAAA CTTATTTGTA ATACAATGAA AAGAAGACAA
    GATGTTTCTC CAAATAGAGA TTTTCTAACA CATTTCTACA ATATAATGCA TTGTGGATTA
    CTTCATATTG ACCAGGATAT TGTCAATACA ATCATCAAAC ACTGCTCACC TCAATTTTTT
    TCACTTGGTT TGCCTGGTGC CACAATGCTT ATTATGGATT TTATTGTAGC AGCTGGTAGA
    GTGGCTTCTT CAGCTTTTCT CAATGCACCA AGAGTAGAAG CACAAGTTCT TCTGGGATCT
    TTGGTTTGCT TTCCCAACTT ATATTGTGAA CTGCCTTCTC TTCATCCCAA CATTCCTGAT
    GTTGCTGTGT CTCAGTTTAC AGATGTTAAG GAACTTATAA TCAAAACTGT ATTAAGCTCG
    GCAAGAGATG AGCCCTCTGG TCCTGCACGA TGTGTAGCAC TTTGTAGTTT AGGTATTTGG
    ATTTGTGAAG AACTAGTCCA TGAGTCTCAT CATCCTCAAA TTAAGGAAGC TCTGAATGTG
    ATTTGTGTTT CCTTAAAGTT TACTAATAAA ACAGTAGCCC ACGTAGCTTG TAACATGCTT
    CACATGCTGG TTCATTATGT ACCTAGACTT CAGATTTACC AGCCTGATTC TCCCTTGAAA
    ATTATTCAAA TCCTAATAGC TACCATCACC CATCTTTTAC CAAGTACAGA GGCTTCATCT
    TATGAAATGG ACAAGAGGTT GGTAGTATCT TTACTTCTCT GCCTTCTGGA CTGGATCATG
    GCCTTACCTC TAAAGACACT GCTCCAACCA TTTCATGCTA CGGGAGCAGA AAGCGATAAA
    ACAGAAAAAT CTGTTCTCAA TTGCATTTAT AAGGTTTTAC ATGGGTGTGT TTATGGAGCT
    CAGTGTTTTA GCAATCCAAG GTATTTTCCC ATGAGCCTCT CTGATTTGGC ATCTGTAGAT
    TATGATCCTT TTATGCATTT GGAAAGTCTG AAAGAGCCTG AGCCTCTGCA CTCTCCTGAC
    TCAGAACGAT CTTCTAAACT CCAGCCAGTA ACAGAAGTGA AAACTCAAAT GCAGCATGGA
    TTAATCTCTA TAGCAGCCCG CACTGTTATT ACACATCTGG TAAATCACCT GGGCCATTAT
    CCAATGAGCG GTGGTCCTGC TATGCTAACA AGTCAGGTGT GTGAAAATCA CGACAATCAT
    TACAGTGAAA GTACTGAACT TTCTCCTGAA CTCTTTGAGA GTCCAAATAT CCAGTTCTTT
    GTGTTAAATA ATACAACCTT AGTGTCCTGT ATCCAGATCA GATCAGAAGA GAATATGCCT
    GGAGGAGGTT TATCTGCTGG CCTTGCATCA GCCAATTCAA ATGTCAGAAT CATAGTACGT
    GATCTCTCTG GAAAATATTC ATGGGATTCT GCTATACTGT ATGGCCCACC TCCTGTAAGT
    GGCTTGTCAG AACCTACATC TTTCATGCTT TCATTGTCTC ACCAAGAGAA GCCAGAAGAG
    CCTCCGACAT CTAATGAATG CTTAGAAGAT ATAACCGTAA AAGATGGACT TTCTCTCCAG
    TTTAAAAGAT TTAGAGAAAC TGTACCAACT TGGGATACAA TAAGAGATGA AGAAGATGTT
    CTTGATGAGC TCTTGCAGTA TTTGGGTGTT ACTAGTCCTG AATGCTTACA GAGAACTGGA
    ATCTCACTTA ATATTCCTGC TCCACAACCT GTGTGCATTT CTGAAAAACA AGAAAATGAT
    GTTATTAATG CTATCCTTAA GCAACATACA GAAGAAAAAG AATTTGTTGA GAAGCACTTT
    AATGACTTAA ACATGAAAGC TGTGGAACAA GATGAACCAA TACCTCAAAA ACCTCAGTCA
    GCATTTTATT ATTGCAGATT GCTTCTTAGT ATATTGGGAA TGAATTCCTG GGACAAACGG
    AGGAGCTTTC ATCTCCTGAA GAAAAATGAA AAGCTACTTA GAGAACTTAG GAACTTGGAT
    TCAAGGCAGT GCCGAGAGAC ACACAAGATT GCAGTATTTT ATGTTGCTGA AGGACAAGAA
    GACAAACACT CCATTCTCAC CAATACAGGA GGAAGTCAAG CATATGAAGA TTTTGTAGCT
    GGTCTTGGTT GGGAGGTAAA TCTTACAAAC CATTGTGGTT TTATGGGAGG ACTACAAAAA
    AACAAAAGCA CTGGATTGAC CACTCCATAT TTTGCTACCT CTACAGTAGA GGTAATATTT
    CACGTGTCAA CAAGAATGCC TTCTGATTCT GATGATTCTT TGACCAAAAA ATTGAGACAT
    TTGGGAAATG ATGAAGTGCA CATTGTTTGG TCAGAGCATA CTAGAGACTA CAGGAGAGGA
    ATTATTCCCA CAGAATTTGG TGATGTCCTT ATTGTAATAT ATCCAATGAA AAATCACATG
    TTCAGTATTC AGATAATGAA AAAACCAGAG GTTCCCTTCT TTGGTCCCCT TTTTGATGGT
    GCTATTGTGA ATGGAAAGGT TCTACCCATT ATGGTTAGAG CAACAGCTAT AAATGCAAGC
    CGTGCTCTGA AATCTCTGAT TCCATTGTAT CAAAACTTCT ATGAGGAGAG AGCACGATAC
    CTGCAAACAA TTGTCCAGCA CCACTTAGAA CCAACAACAT TTGAAGATTT TGCAGCACAG
    GTTTTTTCTC CAGCTCCCTA CCACCATTTA CCATCTGATG CCGTTGGCTC CTACCCAGAG
    ATTCTACCCA GTGAAACTCC CACAGCAACG CAGGTAGATG GGGCTGACCT GGCCTCTCCA
    ATGTCTCCTC GAACTAGCAA AAGCCGCATG TCCATGAAGC TGCGTCGTTC CTCTGGCTCA
    GCCAATAAAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47629
    Clone ID Related Accession (Same CDS sequence) XM_006720098.3 , XM_006720098.2
    Accession Version XM_006720098.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006720098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GTTGGCTCCT ACCCAGAGAT TCTACCCAGT GAAACTCCCA CAGCAACGCA GGTAGATGGG
    GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGTTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720161.1
    CDS176..7948
    Translation

    Target ORF information:

    RefSeq Version XM_006720098.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720098.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GTTGGCTCCT ACCCAGAGAT TCTACCCAGT GAAACTCCCA CAGCAACGCA GGTAGATGGG
    GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGTTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47629
    Clone ID Related Accession (Same CDS sequence) XM_006720098.3 , XM_006720098.2
    Accession Version XM_006720098.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006720098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GTTGGCTCCT ACCCAGAGAT TCTACCCAGT GAAACTCCCA CAGCAACGCA GGTAGATGGG
    GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGTTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720161.1
    CDS470..8242
    Translation

    Target ORF information:

    RefSeq Version XM_006720098.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720098.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GTTGGCTCCT ACCCAGAGAT TCTACCCAGT GAAACTCCCA CAGCAACGCA GGTAGATGGG
    GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGTTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47630
    Clone ID Related Accession (Same CDS sequence) XM_006720099.3 , XM_006720099.2
    Accession Version XM_006720099.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006720099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GGCTCCTACC CAGAGATTCT ACCCAGTGAA ACTCCCACAG CAACGCAGGT AGATGGGGCT
    GACCTGGCCT CTCCAATGTC TCCTCGAACT AGCAAAAGCC GCATGTCCAT GAAGCTGCGT
    CGTTCCTCTG GCTCAGCCAA TAAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720162.1
    CDS176..7945
    Translation

    Target ORF information:

    RefSeq Version XM_006720099.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720099.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GGCTCCTACC CAGAGATTCT ACCCAGTGAA ACTCCCACAG CAACGCAGGT AGATGGGGCT
    GACCTGGCCT CTCCAATGTC TCCTCGAACT AGCAAAAGCC GCATGTCCAT GAAGCTGCGT
    CGTTCCTCTG GCTCAGCCAA TAAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47630
    Clone ID Related Accession (Same CDS sequence) XM_006720099.3 , XM_006720099.2
    Accession Version XM_006720099.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006720099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GGCTCCTACC CAGAGATTCT ACCCAGTGAA ACTCCCACAG CAACGCAGGT AGATGGGGCT
    GACCTGGCCT CTCCAATGTC TCCTCGAACT AGCAAAAGCC GCATGTCCAT GAAGCTGCGT
    CGTTCCTCTG GCTCAGCCAA TAAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720162.1
    CDS470..8239
    Translation

    Target ORF information:

    RefSeq Version XM_006720099.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720099.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GGCTCCTACC CAGAGATTCT ACCCAGTGAA ACTCCCACAG CAACGCAGGT AGATGGGGCT
    GACCTGGCCT CTCCAATGTC TCCTCGAACT AGCAAAAGCC GCATGTCCAT GAAGCTGCGT
    CGTTCCTCTG GCTCAGCCAA TAAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47631
    Clone ID Related Accession (Same CDS sequence) XM_006720100.3 , XM_006720100.4
    Accession Version XM_006720100.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006720100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG TTGGCTCCTA CCCAGAGATT
    CTACCCAGTG AAACTCCCAC AGCAACGCAG GTAGATGGGG CTGACCTGGC CTCTCCAATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGTTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720163.1
    CDS470..8101
    Translation

    Target ORF information:

    RefSeq Version XM_006720100.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720100.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG TTGGCTCCTA CCCAGAGATT
    CTACCCAGTG AAACTCCCAC AGCAACGCAG GTAGATGGGG CTGACCTGGC CTCTCCAATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGTTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47631
    Clone ID Related Accession (Same CDS sequence) XM_006720100.3 , XM_006720100.4
    Accession Version XM_006720100.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006720100.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG TTGGCTCCTA CCCAGAGATT
    CTACCCAGTG AAACTCCCAC AGCAACGCAG GTAGATGGGG CTGACCTGGC CTCTCCAATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGTTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720163.1
    CDS176..7807
    Translation

    Target ORF information:

    RefSeq Version XM_006720100.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720100.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGATAT TGATGATGCT
    CAAATACTTC CCCGCTCAAC TAGAGTCAGA CATTTTTCAC AAAGTGAAGA AACTGGAAAT
    GAAGTTTTTG GTGCTTTGAA TGAGGAGCAG CCATTGCCTC GAAGTAGCAG CACTTCTGAC
    ATCTTGGAAC CATTCACTGT TGAACGAGCC AAAGGTGCAG TTCCTGTCAT TGACAGTTCA
    TCCCGTCATG CACCAAGCTT GCAGAGTTCC ACAGAGGCTT CTTCAATAAC TAGATCCACT
    GAAAGCCACA TCACTGATAC TCATAGTAGA GAGTCTTCTC TGGAAGTTGG TGATAGCATA
    TATGACCATC TTTGTCATTT AATAGGTCCA GTAGAACTTG CAGATTCAGC TTTTGAACAA
    ATCCAGTACA TTGACCTTGA AGGAGATGAT GATCTTCTTT CCACCCTGAA AGAATATTTT
    AAGGAAAACC AGGAAAATCA TAGTAAAAAT GAGACAGGGA AAGACCCAGC TTCTCAGGAA
    GTGACTATTG CAGTAAATAG GGGTGAAAGA TTATCCTTAG ATAAATTAGA ATGCACAGAT
    CAGGAAACTG AATCAGAAAA TATCACCTCT TTTGTTGGGA CTCCTGAAAA CCTACAGTTT
    CAGAAAGAAC CAAACTCAGC TGTCTTCATG AGTAATATCG CACCTAACCA GTCAGACAGT
    TTTTTTAGAA CACAAACTTC TGAAAAATCT AAGCAACTAA ACACTGATAA ACAGCCATCA
    GAGCCTAGTT TAGATAGCCC TTGTGATAAA GAAAAAAGGA AACATCTGTA CAGACAAGCA
    GCCACAGAAT TAGATGCTTG TGTTGATGTA ACACTAGTTG AAAAGCTTAA GAGTGTGCAA
    ATTAATGAAA AAATCACTGT CCCACATGTT ATGCGTGCCA AGAAAGCAAC ACTAAAAGCA
    CCTGTTAACC GCAGAATGCC TCATGTTACA AGTACTAGCA AACTTTCTCC AACTAAAAGG
    AGCTTGTCTG AAACAGTAAC TCATAGAGCC AAAATCATGA AAATTGCTAC TAAAAAACGA
    AATAGTGTTC ATGTTACTTT TAGGCCATCC ACTGAGTCCG TTCAGTTTTA TAATCCTCTG
    GAAAACAAAG AGGCTCCATG GAAGATGAGA CTGCGGAAGC TCGGTGGCTT TAGTAGTGGT
    AGCAGCAATA GCAGCACTAG CAACACCCAT ACCAGCACAA ATAGTGCTAC AGAGCTAGTA
    AAACCTGGTG TGTACAGACC TCTAGATACA CTTGGTACTG CATCAGTAAG CAGCAAAACA
    GTGAAGGAAT CCACAGAGAT TCCCACCACC ATATTACAAA AAGAAGGAAT TGCAAGTAGT
    CAATTAGGTA GTCGTAGTAC TCTTAGGTCA TCAAGTCATG AGGCAGGACT ACAGCAGGGC
    TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT
    TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT
    GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTCAATAAAG AGGACATGAG CCAAAAACTG
    CCTCCTCTTA ATAGTGATAT TGGCGGCAGC AGTGCTAATG TTCCTGATCT GATGGATGAG
    TTTATAGCAG AACGACTTCG AAGTGGTAAT GCCTCGACTA TGACAAGAAG AGGAAGTAGT
    CCAGGCAGCC TGGAAATTCC CAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGCCCTATTG ATGACCCAGG TGTGCCCTCA GAATGGACTT CTCCTGCCAG TGCAGGGAGC
    AGTGATCTTA TCAGCTCAGA TAGTCATTCG GATTCTTTCA GCGCTTTCCA ATATGATGGC
    CGAAAATTTG ACAATTTTGG CTTTGGAACC GACACTGGGG TTACGTCCTC TGCTGATGTG
    GATTCAGGTT CTGGCCATCA TCAGAGTGCT GAAGAGCAGG AAGTGGCTAG TCTAACTACT
    CTTCATATAG ATTCTGAAAC AAGCAGTCTT AATCAGCAAG CTTTCTCTGC TGAAGTTGCA
    ACTATTACTG GTTCAGAAAG TGCTTCCCCA GTCCACTCAC CTCTGGGCTC CAGGTCACAG
    ACTCCTTCCC CTTCTACATT GAATATAGAT CACATGGAAC AGAAGGATCT GCAGCTCGAC
    GAGAAGCTCC ACCACTCTGT TCTTCAGACA CCAGATGATC TAGAAATTAG TGAATTTCCA
    TCAGAATGTT GTAGTGTGAT GGCAGGAGGT ACTCTGACTG GATGGCATGC TGATGTTGCT
    ACTGTAATGT GGCGAAGAAT GCTAGGCATT TTGGGAGATG TAAATTCAAT CATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCTAAGATT
    AGAGATAACC TTGGCATTTC AACTGATAAC CTGACCTCCC CTTCTCCACC AGTTTTAATT
    CCTCCACTGA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAATAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAAAG AAGACAAGAT
    GTTTCTCCAA ATAGAGATTT TCTAACACAT TTCTACAATA TAATGCATTG TGGATTACTT
    CATATTGACC AGGATATTGT CAATACAATC ATCAAACACT GCTCACCTCA ATTTTTTTCA
    CTTGGTTTGC CTGGTGCCAC AATGCTTATT ATGGATTTTA TTGTAGCAGC TGGTAGAGTG
    GCTTCTTCAG CTTTTCTCAA TGCACCAAGA GTAGAAGCAC AAGTTCTTCT GGGATCTTTG
    GTTTGCTTTC CCAACTTATA TTGTGAACTG CCTTCTCTTC ATCCCAACAT TCCTGATGTT
    GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATCA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GTAGTTTAGG TATTTGGATT
    TGTGAAGAAC TAGTCCATGA GTCTCATCAT CCTCAAATTA AGGAAGCTCT GAATGTGATT
    TGTGTTTCCT TAAAGTTTAC TAATAAAACA GTAGCCCACG TAGCTTGTAA CATGCTTCAC
    ATGCTGGTTC ATTATGTACC TAGACTTCAG ATTTACCAGC CTGATTCTCC CTTGAAAATT
    ATTCAAATCC TAATAGCTAC CATCACCCAT CTTTTACCAA GTACAGAGGC TTCATCTTAT
    GAAATGGACA AGAGGTTGGT AGTATCTTTA CTTCTCTGCC TTCTGGACTG GATCATGGCC
    TTACCTCTAA AGACACTGCT CCAACCATTT CATGCTACGG GAGCAGAAAG CGATAAAACA
    GAAAAATCTG TTCTCAATTG CATTTATAAG GTTTTACATG GGTGTGTTTA TGGAGCTCAG
    TGTTTTAGCA ATCCAAGGTA TTTTCCCATG AGCCTCTCTG ATTTGGCATC TGTAGATTAT
    GATCCTTTTA TGCATTTGGA AAGTCTGAAA GAGCCTGAGC CTCTGCACTC TCCTGACTCA
    GAACGATCTT CTAAACTCCA GCCAGTAACA GAAGTGAAAA CTCAAATGCA GCATGGATTA
    ATCTCTATAG CAGCCCGCAC TGTTATTACA CATCTGGTAA ATCACCTGGG CCATTATCCA
    ATGAGCGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCACGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAACTC TTTGAGAGTC CAAATATCCA GTTCTTTGTG
    TTAAATAATA CAACCTTAGT GTCCTGTATC CAGATCAGAT CAGAAGAGAA TATGCCTGGA
    GGAGGTTTAT CTGCTGGCCT TGCATCAGCC AATTCAAATG TCAGAATCAT AGTACGTGAT
    CTCTCTGGAA AATATTCATG GGATTCTGCT ATACTGTATG GCCCACCTCC TGTAAGTGGC
    TTGTCAGAAC CTACATCTTT CATGCTTTCA TTGTCTCACC AAGAGAAGCC AGAAGAGCCT
    CCGACATCTA ATGAATGCTT AGAAGATATA ACCGTAAAAG ATGGACTTTC TCTCCAGTTT
    AAAAGATTTA GAGAAACTGT ACCAACTTGG GATACAATAA GAGATGAAGA AGATGTTCTT
    GATGAGCTCT TGCAGTATTT GGGTGTTACT AGTCCTGAAT GCTTACAGAG AACTGGAATC
    TCACTTAATA TTCCTGCTCC ACAACCTGTG TGCATTTCTG AAAAACAAGA AAATGATGTT
    ATTAATGCTA TCCTTAAGCA ACATACAGAA GAAAAAGAAT TTGTTGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCTGT GGAACAAGAT GAACCAATAC CTCAAAAACC TCAGTCAGCA
    TTTTATTATT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTACTTAGAG AACTTAGGAA CTTGGATTCA
    AGGCAGTGCC GAGAGACACA CAAGATTGCA GTATTTTATG TTGCTGAAGG ACAAGAAGAC
    AAACACTCCA TTCTCACCAA TACAGGAGGA AGTCAAGCAT ATGAAGATTT TGTAGCTGGT
    CTTGGTTGGG AGGTAAATCT TACAAACCAT TGTGGTTTTA TGGGAGGACT ACAAAAAAAC
    AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAGGT AATATTTCAC
    GTGTCAACAA GAATGCCTTC TGATTCTGAT GATTCTTTGA CCAAAAAATT GAGACATTTG
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAGCATACTA GAGACTACAG GAGAGGAATT
    ATTCCCACAG AATTTGGTGA TGTCCTTATT GTAATATATC CAATGAAAAA TCACATGTTC
    AGTATTCAGA TAATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCCCTTTT TGATGGTGCT
    ATTGTGAATG GAAAGGTTCT ACCCATTATG GTTAGAGCAA CAGCTATAAA TGCAAGCCGT
    GCTCTGAAAT CTCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGATACCTG
    CAAACAATTG TCCAGCACCA CTTAGAACCA ACAACATTTG AAGATTTTGC AGCACAGGTT
    TTTTCTCCAG CTCCCTACCA CCATTTACCA TCTGATGCCG TTGGCTCCTA CCCAGAGATT
    CTACCCAGTG AAACTCCCAC AGCAACGCAG GTAGATGGGG CTGACCTGGC CTCTCCAATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGTTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47632
    Clone ID Related Accession (Same CDS sequence) NM_001346249.1 , XM_006720101.2 , NM_001346249.2 , XM_006720101.3 , XM_011536615.3 , XM_011536615.2
    Accession Version XM_006720101.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006720101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720164.1
    CDS470..8098
    Translation

    Target ORF information:

    RefSeq Version XM_006720101.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720101.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47632
    Clone ID Related Accession (Same CDS sequence) NM_001346249.1 , XM_006720101.2 , NM_001346249.2 , XM_006720101.3 , XM_011536615.3 , XM_011536615.2
    Accession Version XM_006720101.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006720101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
    3541
    3601
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    3721
    3781
    3841
    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720164.1
    CDS176..7804
    Translation

    Target ORF information:

    RefSeq Version XM_006720101.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720101.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47632
    Clone ID Related Accession (Same CDS sequence) NM_001346249.1 , XM_006720101.2 , NM_001346249.2 , XM_006720101.3 , XM_011536615.3 , XM_011536615.2
    Accession Version XM_011536615.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011536615.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534917.1
    CDS176..7804
    Translation

    Target ORF information:

    RefSeq Version XM_011536615.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011536615.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47632
    Clone ID Related Accession (Same CDS sequence) NM_001346249.1 , XM_006720101.2 , NM_001346249.2 , XM_006720101.3 , XM_011536615.3 , XM_011536615.2
    Accession Version XM_011536615.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536615.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534917.1
    CDS470..8098
    Translation

    Target ORF information:

    RefSeq Version XM_011536615.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011536615.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGGTGCA
    GTTCCTGTCA TTGACAGTTC ATCCCGTCAT GCACCAAGCT TGCAGAGTTC CACAGAGGCT
    TCTTCAATAA CTAGATCCAC TGAAAGCCAC ATCACTGATA CTCATAGTAG AGAGTCTTCT
    CTGGAAGTTG GTGATAGCAT ATATGACCAT CTTTGTCATT TAATAGGTCC AGTAGAACTT
    GCAGATTCAG CTTTTGAACA AATCCAGTAC ATTGACCTTG AAGGAGATGA TGATCTTCTT
    TCCACCCTGA AAGAATATTT TAAGGAAAAC CAGGAAAATC ATAGTAAAAA TGAGACAGGG
    AAAGACCCAG CTTCTCAGGA AGTGACTATT GCAGTAAATA GGGGTGAAAG ATTATCCTTA
    GATAAATTAG AATGCACAGA TCAGGAAACT GAATCAGAAA ATATCACCTC TTTTGTTGGG
    ACTCCTGAAA ACCTACAGTT TCAGAAAGAA CCAAACTCAG CTGTCTTCAT GAGTAATATC
    GCACCTAACC AGTCAGACAG TTTTTTTAGA ACACAAACTT CTGAAAAATC TAAGCAACTA
    AACACTGATA AACAGCCATC AGAGCCTAGT TTAGATAGCC CTTGTGATAA AGAAAAAAGG
    AAACATCTGT ACAGACAAGC AGCCACAGAA TTAGATGCTT GTGTTGATGT AACACTAGTT
    GAAAAGCTTA AGAGTGTGCA AATTAATGAA AAAATCACTG TCCCACATGT TATGCGTGCC
    AAGAAAGCAA CACTAAAAGC ACCTGTTAAC CGCAGAATGC CTCATGTTAC AAGTACTAGC
    AAACTTTCTC CAACTAAAAG GAGCTTGTCT GAAACAGTAA CTCATAGAGC CAAAATCATG
    AAAATTGCTA CTAAAAAACG AAATAGTGTT CATGTTACTT TTAGGCCATC CACTGAGTCC
    GTTCAGTTTT ATAATCCTCT GGAAAACAAA GAGGCTCCAT GGAAGATGAG ACTGCGGAAG
    CTCGGTGGCT TTAGTAGTGG TAGCAGCAAT AGCAGCACTA GCAACACCCA TACCAGCACA
    AATAGTGCTA CAGAGCTAGT AAAACCTGGT GTGTACAGAC CTCTAGATAC ACTTGGTACT
    GCATCAGTAA GCAGCAAAAC AGTGAAGGAA TCCACAGAGA TTCCCACCAC CATATTACAA
    AAAGAAGGAA TTGCAAGTAG TCAATTAGGT AGTCGTAGTA CTCTTAGGTC ATCAAGTCAT
    GAGGCAGGAC TACAGCAGGG CTCCCTGGGT GGCGTTTATA AAACTGTTGT ACATGCTCTT
    TCGAAGCCGA AGGCAAATGT TTCCCCACAG AGGCAGAACA GAATGCCACC AGAGGCTCCA
    CTGAGGGATC TGTACAGTCA TGTAATGGGC TATTTTGGAA GGAAAGCTGC AGTCAATAAA
    GAGGACATGA GCCAAAAACT GCCTCCTCTT AATAGTGATA TTGGCGGCAG CAGTGCTAAT
    GTTCCTGATC TGATGGATGA GTTTATAGCA GAACGACTTC GAAGTGGTAA TGCCTCGACT
    ATGACAAGAA GAGGAAGTAG TCCAGGCAGC CTGGAAATTC CCAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGCCCTATT GATGACCCAG GTGTGCCCTC AGAATGGACT
    TCTCCTGCCA GTGCAGGGAG CAGTGATCTT ATCAGCTCAG ATAGTCATTC GGATTCTTTC
    AGCGCTTTCC AATATGATGG CCGAAAATTT GACAATTTTG GCTTTGGAAC CGACACTGGG
    GTTACGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGTGC TGAAGAGCAG
    GAAGTGGCTA GTCTAACTAC TCTTCATATA GATTCTGAAA CAAGCAGTCT TAATCAGCAA
    GCTTTCTCTG CTGAAGTTGC AACTATTACT GGTTCAGAAA GTGCTTCCCC AGTCCACTCA
    CCTCTGGGCT CCAGGTCACA GACTCCTTCC CCTTCTACAT TGAATATAGA TCACATGGAA
    CAGAAGGATC TGCAGCTCGA CGAGAAGCTC CACCACTCTG TTCTTCAGAC ACCAGATGAT
    CTAGAAATTA GTGAATTTCC ATCAGAATGT TGTAGTGTGA TGGCAGGAGG TACTCTGACT
    GGATGGCATG CTGATGTTGC TACTGTAATG TGGCGAAGAA TGCTAGGCAT TTTGGGAGAT
    GTAAATTCAA TCATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCTAAGAT TAGAGATAAC CTTGGCATTT CAACTGATAA CCTGACCTCC
    CCTTCTCCAC CAGTTTTAAT TCCTCCACTG AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAATA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAAA GAAGACAAGA TGTTTCTCCA AATAGAGATT TTCTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTACT TCATATTGAC CAGGATATTG TCAATACAAT CATCAAACAC
    TGCTCACCTC AATTTTTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTTAT TATGGATTTT
    ATTGTAGCAG CTGGTAGAGT GGCTTCTTCA GCTTTTCTCA ATGCACCAAG AGTAGAAGCA
    CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACTTAT ATTGTGAACT GCCTTCTCTT
    CATCCCAACA TTCCTGATGT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATC
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGTAGTTTAG GTATTTGGAT TTGTGAAGAA CTAGTCCATG AGTCTCATCA TCCTCAAATT
    AAGGAAGCTC TGAATGTGAT TTGTGTTTCC TTAAAGTTTA CTAATAAAAC AGTAGCCCAC
    GTAGCTTGTA ACATGCTTCA CATGCTGGTT CATTATGTAC CTAGACTTCA GATTTACCAG
    CCTGATTCTC CCTTGAAAAT TATTCAAATC CTAATAGCTA CCATCACCCA TCTTTTACCA
    AGTACAGAGG CTTCATCTTA TGAAATGGAC AAGAGGTTGG TAGTATCTTT ACTTCTCTGC
    CTTCTGGACT GGATCATGGC CTTACCTCTA AAGACACTGC TCCAACCATT TCATGCTACG
    GGAGCAGAAA GCGATAAAAC AGAAAAATCT GTTCTCAATT GCATTTATAA GGTTTTACAT
    GGGTGTGTTT ATGGAGCTCA GTGTTTTAGC AATCCAAGGT ATTTTCCCAT GAGCCTCTCT
    GATTTGGCAT CTGTAGATTA TGATCCTTTT ATGCATTTGG AAAGTCTGAA AGAGCCTGAG
    CCTCTGCACT CTCCTGACTC AGAACGATCT TCTAAACTCC AGCCAGTAAC AGAAGTGAAA
    ACTCAAATGC AGCATGGATT AATCTCTATA GCAGCCCGCA CTGTTATTAC ACATCTGGTA
    AATCACCTGG GCCATTATCC AATGAGCGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCACG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAACT CTTTGAGAGT
    CCAAATATCC AGTTCTTTGT GTTAAATAAT ACAACCTTAG TGTCCTGTAT CCAGATCAGA
    TCAGAAGAGA ATATGCCTGG AGGAGGTTTA TCTGCTGGCC TTGCATCAGC CAATTCAAAT
    GTCAGAATCA TAGTACGTGA TCTCTCTGGA AAATATTCAT GGGATTCTGC TATACTGTAT
    GGCCCACCTC CTGTAAGTGG CTTGTCAGAA CCTACATCTT TCATGCTTTC ATTGTCTCAC
    CAAGAGAAGC CAGAAGAGCC TCCGACATCT AATGAATGCT TAGAAGATAT AACCGTAAAA
    GATGGACTTT CTCTCCAGTT TAAAAGATTT AGAGAAACTG TACCAACTTG GGATACAATA
    AGAGATGAAG AAGATGTTCT TGATGAGCTC TTGCAGTATT TGGGTGTTAC TAGTCCTGAA
    TGCTTACAGA GAACTGGAAT CTCACTTAAT ATTCCTGCTC CACAACCTGT GTGCATTTCT
    GAAAAACAAG AAAATGATGT TATTAATGCT ATCCTTAAGC AACATACAGA AGAAAAAGAA
    TTTGTTGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCTG TGGAACAAGA TGAACCAATA
    CCTCAAAAAC CTCAGTCAGC ATTTTATTAT TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTACTTAGA
    GAACTTAGGA ACTTGGATTC AAGGCAGTGC CGAGAGACAC ACAAGATTGC AGTATTTTAT
    GTTGCTGAAG GACAAGAAGA CAAACACTCC ATTCTCACCA ATACAGGAGG AAGTCAAGCA
    TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGGTAAATC TTACAAACCA TTGTGGTTTT
    ATGGGAGGAC TACAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCT
    ACAGTAGAGG TAATATTTCA CGTGTCAACA AGAATGCCTT CTGATTCTGA TGATTCTTTG
    ACCAAAAAAT TGAGACATTT GGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAGCATACT
    AGAGACTACA GGAGAGGAAT TATTCCCACA GAATTTGGTG ATGTCCTTAT TGTAATATAT
    CCAATGAAAA ATCACATGTT CAGTATTCAG ATAATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCCCTTT TTGATGGTGC TATTGTGAAT GGAAAGGTTC TACCCATTAT GGTTAGAGCA
    ACAGCTATAA ATGCAAGCCG TGCTCTGAAA TCTCTGATTC CATTGTATCA AAACTTCTAT
    GAGGAGAGAG CACGATACCT GCAAACAATT GTCCAGCACC ACTTAGAACC AACAACATTT
    GAAGATTTTG CAGCACAGGT TTTTTCTCCA GCTCCCTACC ACCATTTACC ATCTGATGCC
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47634
    Clone ID Related Accession (Same CDS sequence) XM_005267492.4 , XM_005267492.3
    Accession Version XM_005267492.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005267492.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGGCTCCT ACCCAGAGAT TCTACCCAGT GAAACTCCCA CAGCAACGCA GGTAGATGGG
    GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGTTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005267549.1
    CDS176..6568
    Translation

    Target ORF information:

    RefSeq Version XM_005267492.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic subunit alpha 1 (RALGAPA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005267492.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGGCTCCT ACCCAGAGAT TCTACCCAGT GAAACTCCCA CAGCAACGCA GGTAGATGGG
    GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGTTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47634
    Clone ID Related Accession (Same CDS sequence) XM_005267492.4 , XM_005267492.3
    Accession Version XM_005267492.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005267492.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGGCTCCT ACCCAGAGAT TCTACCCAGT GAAACTCCCA CAGCAACGCA GGTAGATGGG
    GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGTTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005267549.1
    CDS470..6862
    Translation

    Target ORF information:

    RefSeq Version XM_005267492.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005267492.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCCA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTAGACACC 
    AAGAAGGACG CACTGACTCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGATCTTA AACAGTTTTT CGACCAACAT TTTTCACATA TATACTATGT GTTCTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AGTTTCATAG TATTGGATTG ATTTTAAAGA AGCTACTTCA CACAGGAAAC
    TCCTTAAAGA TTAGGCGGGA AGGTGTTCGT CTTTTCTTAC TATGGTTGCA AGCTCTTCAG
    AATAACTGTA GCAAAGAACA GCTCTGGATG TTTTCATGCT TAATCCCTGG ATTTTCAGCA
    CCACAGTCTG AACATGGACC TCGAACTTTA GATAATCTCA TTAATCCTCC ACTCAACCTT
    CAAGAAACTC AAGTCACTAT AGAAGAAATC ACTCCTCTTG TCCCCCCACA ATCAGGAGAT
    AAAGGGCAAG AAGATCTCAC AAGCTATTTT CTTGAAGCAC TTCTAAAATA CATAGTCATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAATA TTACTTGCCT TATATTTTTC CAAACATCTG TAAGGAAAAC
    AGTTTATATC ATCCTATACT TGACATCCCG CAGATGAGAC CAAAGCCACA TTATGTCGTG
    ATAAAGAAAG ATGCTGAAAC CAATGAAGCA ATCTATTGTA CAAAGGAGCC TTTCATTAAG
    GCTCGTGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATACA
    GGACCTCATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTAG TATCCAGAGA AGAAAGCAAA AATGATAATG CTGATAAAAC AGACAGAACT
    ACAGAACCCG AACAGTCTCA TTCCAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCA
    TCTAGTCTCT GTAGTATTGA TGAAGAACAT CTCACAGACA TTGAAATAGT TCGCAGAGTT
    TTTTCTTCTA AAAGGAGTAA TGTAAACTTT GTGACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTATATCA AGAATGGATC
    CAACAAGAGG AAAAACCTTT GTTCATGCAA GAGCCTGAAG AAATTGTGAT CACTTCTTCA
    GACCTCCCTT GCATTGAAAA TGTCACAGAC CATGATATTT CAATGGAAGA AGGAGAAAAA
    AGAGAAGAGG AAAATGGGAC CAATACTGCT GATCATGTTC GAAATTCCAG TTGGGCAAAA
    AACGGCTCCT ACCAAGGTGC TCTTCATAAC GCCTCTGAAG AAGCCACAGA ACAAAACATA
    CGAGCTGGTA CCCAGGCAGT TTTGCAGGTG TTTATTATAA ACTCATCAAA TATATTTCTT
    CTTGAACCTG CAAATGAAAT AAAAAATCTT CTGGATGAAC ACACAGATAT GTGTAAACGC
    ATTCTTAACA TTTATCGGTA CATGGTTGTA CAAGTATCAA TGGACAAAAA GACTTGGGAA
    CAGATGCTGC TTGTGTTGCT CAGAGTCACG GAATCTGTAC TGAAGATGCC ATCACAAGCT
    TTTCTACAGT TCCAAGGGAA AAAAAATATG ACCTTGGCAG GTCGACTTGC AGGACCACTT
    TTCCAGACCC TTATAGTTGC CTGGATCAAA GCAAACCTAA ATGTGTACAT CTCCCGAGAA
    CTTTGGGATG ACTTACTGTC AGTATTGTCA TCGCTGACCT ATTGGGAAGA GTTGGCCACT
    GAGTGGTCAC TGACTATGGA GACATTAACT AAAGTTTTAG CTAGGAATTT ATATAGTTTG
    GATCTCAGTG ATTTACCATT GGATAAGCTG AGTGAACAGA AACAAAAAAA GCACAAAGGG
    AAAGGAGTTG GACATGAATT TCAGAAAGTT TCAGTTGACA AGTCATTTTC TAGAGGATGG
    AGTCGTGATC AGCCTGGCCA AGCCCCAATG AGACAGAGGA GTGCAACAAC CACTGGTTCT
    CCAGGAACCG AAAAGGCGAG GAGTATAGTA CGGCAAAAAA CTGTCGCCAT GAGAAGCCGA
    TCCATTGGTG AATGTGCTCT GCCATCGGCC TATATACGCA GTGCTAAAAG TGCTCCTGTT
    CTGATCCATA CTTCCAAACC CTTCTTGCCT GATATTGTTC TCACTCCCCT TTCTGATGAG
    CTTTCAGATA TTGATGATGC TCAAATACTT CCCCGCTCAA CTAGAGTCAG ACATTTTTCA
    CAAAGTGAAG AAACTGGAAA TGAAGTTTTT GGTGCTTTGA ATGAGGAGCA GCCATTGCCT
    CGAAGTAGCA GCACTTCTGA CATCTTGGAA CCATTCACTG TTGAACGAGC CAAAGTCAAT
    AAAGAGGACA TGAGCCAAAA ACTGCCTCCT CTTAATAGTG ATATTGGCGG CAGCAGTGCT
    AATGTTCCTG ATCTGATGGA TGAGTTTATA GCAGAACGAC TTCGAAGTGG TAATGCCTCG
    ACTATGACAA GAAGAGGAAG TAGTCCAGGC AGCCTGGAAA TTCCCAAAGA CCTCCCTGAT
    ATTCTAAACA AGCAGAACCA GATGCGCCCT ATTGATGACC CAGGTGTGCC CTCAGAATGG
    ACTTCTCCTG CCAGTGCAGG GAGCAGTGAT CTTATCAGCT CAGATAGTCA TTCGGATTCT
    TTCAGCGCTT TCCAATATGA TGGCCGAAAA TTTGACAATT TTGGCTTTGG AACCGACACT
    GGGGTTACGT CCTCTGCTGA TGTGGATTCA GGTTCTGGCC ATCATCAGAG TGCTGAAGAG
    CAGGAAGTGG CTAGTCTAAC TACTCTTCAT ATAGATTCTG AAACAAGCAG TCTTAATCAG
    CAAGCTTTCT CTGCTGAAGT TGCAACTATT ACTGGTTCAG AAAGTGCTTC CCCAGTCCAC
    TCACCTCTGG GCTCCAGGTC ACAGACTCCT TCCCCTTCTA CATTGAATAT AGATCACATG
    GAACAGAAGG ATCTGCAGCT CGACGAGAAG CTCCACCACT CTGTTCTTCA GACACCAGAT
    GATCTAGAAA TTAGTGAATT TCCATCAGAA TGTTGTAGTG TGATGGCAGG AGGTACTCTG
    ACTGGATGGC ATGCTGATGT TGCTACTGTA ATGTGGCGAA GAATGCTAGG CATTTTGGGA
    GATGTAAATT CAATCATGGA TCCTGAAATA CATGCTCAAG TTTTTGATTA CCTCTGTGAA
    CTTTGGCAGA ATCTAGCTAA GATTAGAGAT AACCTTGGCA TTTCAACTGA TAACCTGACC
    TCCCCTTCTC CACCAGTTTT AATTCCTCCA CTGAGAATTC TTACACCTTG GCTTTTTAAG
    GCAACCATGT TGACTGATAA ATATAAACAA GGTAAATTAC ATGCATATAA ACTTATTTGT
    AATACAATGA AAAGAAGACA AGATGTTTCT CCAAATAGAG ATTTTCTAAC ACATTTCTAC
    AATATAATGC ATTGTGGATT ACTTCATATT GACCAGGATA TTGTCAATAC AATCATCAAA
    CACTGCTCAC CTCAATTTTT TTCACTTGGT TTGCCTGGTG CCACAATGCT TATTATGGAT
    TTTATTGTAG CAGCTGGTAG AGTGGCTTCT TCAGCTTTTC TCAATGCACC AAGAGTAGAA
    GCACAAGTTC TTCTGGGATC TTTGGTTTGC TTTCCCAACT TATATTGTGA ACTGCCTTCT
    CTTCATCCCA ACATTCCTGA TGTTGCTGTG TCTCAGTTTA CAGATGTTAA GGAACTTATA
    ATCAAAACTG TATTAAGCTC GGCAAGAGAT GAGCCCTCTG GTCCTGCACG ATGTGTAGCA
    CTTTGTAGTT TAGGTATTTG GATTTGTGAA GAACTAGTCC ATGAGTCTCA TCATCCTCAA
    ATTAAGGAAG CTCTGAATGT GATTTGTGTT TCCTTAAAGT TTACTAATAA AACAGTAGCC
    CACGTAGCTT GTAACATGCT TCACATGCTG GTTCATTATG TACCTAGACT TCAGATTTAC
    CAGCCTGATT CTCCCTTGAA AATTATTCAA ATCCTAATAG CTACCATCAC CCATCTTTTA
    CCAAGTACAG AGGCTTCATC TTATGAAATG GACAAGAGGT TGGTAGTATC TTTACTTCTC
    TGCCTTCTGG ACTGGATCAT GGCCTTACCT CTAAAGACAC TGCTCCAACC ATTTCATGCT
    ACGGGAGCAG AAAGCGATAA AACAGAAAAA TCTGTTCTCA ATTGCATTTA TAAGGTTTTA
    CATGGGTGTG TTTATGGAGC TCAGTGTTTT AGCAATCCAA GGTATTTTCC CATGAGCCTC
    TCTGATTTGG CATCTGTAGA TTATGATCCT TTTATGCATT TGGAAAGTCT GAAAGAGCCT
    GAGCCTCTGC ACTCTCCTGA CTCAGAACGA TCTTCTAAAC TCCAGCCAGT AACAGAAGTG
    AAAACTCAAA TGCAGCATGG ATTAATCTCT ATAGCAGCCC GCACTGTTAT TACACATCTG
    GTAAATCACC TGGGCCATTA TCCAATGAGC GGTGGTCCTG CTATGCTAAC AAGTCAGGTG
    TGTGAAAATC ACGACAATCA TTACAGTGAA AGTACTGAAC TTTCTCCTGA ACTCTTTGAG
    AGTCCAAATA TCCAGTTCTT TGTGTTAAAT AATACAACCT TAGTGTCCTG TATCCAGATC
    AGATCAGAAG AGAATATGCC TGGAGGAGGT TTATCTGCTG GCCTTGCATC AGCCAATTCA
    AATGTCAGAA TCATAGTACG TGATCTCTCT GGAAAATATT CATGGGATTC TGCTATACTG
    TATGGCCCAC CTCCTGTAAG TGGCTTGTCA GAACCTACAT CTTTCATGCT TTCATTGTCT
    CACCAAGAGA AGCCAGAAGA GCCTCCGACA TCTAATGAAT GCTTAGAAGA TATAACCGTA
    AAAGATGGAC TTTCTCTCCA GTTTAAAAGA TTTAGAGAAA CTGTACCAAC TTGGGATACA
    ATAAGAGATG AAGAAGATGT TCTTGATGAG CTCTTGCAGT ATTTGGGTGT TACTAGTCCT
    GAATGCTTAC AGAGAACTGG AATCTCACTT AATATTCCTG CTCCACAACC TGTGTGCATT
    TCTGAAAAAC AAGAAAATGA TGTTATTAAT GCTATCCTTA AGCAACATAC AGAAGAAAAA
    GAATTTGTTG AGAAGCACTT TAATGACTTA AACATGAAAG CTGTGGAACA AGATGAACCA
    ATACCTCAAA AACCTCAGTC AGCATTTTAT TATTGCAGAT TGCTTCTTAG TATATTGGGA
    ATGAATTCCT GGGACAAACG GAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGCTACTT
    AGAGAACTTA GGAACTTGGA TTCAAGGCAG TGCCGAGAGA CACACAAGAT TGCAGTATTT
    TATGTTGCTG AAGGACAAGA AGACAAACAC TCCATTCTCA CCAATACAGG AGGAAGTCAA
    GCATATGAAG ATTTTGTAGC TGGTCTTGGT TGGGAGGTAA ATCTTACAAA CCATTGTGGT
    TTTATGGGAG GACTACAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC
    TCTACAGTAG AGGTAATATT TCACGTGTCA ACAAGAATGC CTTCTGATTC TGATGATTCT
    TTGACCAAAA AATTGAGACA TTTGGGAAAT GATGAAGTGC ACATTGTTTG GTCAGAGCAT
    ACTAGAGACT ACAGGAGAGG AATTATTCCC ACAGAATTTG GTGATGTCCT TATTGTAATA
    TATCCAATGA AAAATCACAT GTTCAGTATT CAGATAATGA AAAAACCAGA GGTTCCCTTC
    TTTGGTCCCC TTTTTGATGG TGCTATTGTG AATGGAAAGG TTCTACCCAT TATGGTTAGA
    GCAACAGCTA TAAATGCAAG CCGTGCTCTG AAATCTCTGA TTCCATTGTA TCAAAACTTC
    TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTCCAGC ACCACTTAGA ACCAACAACA
    TTTGAAGATT TTGCAGCACA GGTTTTTTCT CCAGCTCCCT ACCACCATTT ACCATCTGAT
    GCCGGCTCCT ACCCAGAGAT TCTACCCAGT GAAACTCCCA CAGCAACGCA GGTAGATGGG
    GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGTTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.