RALGAPA1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	RALGAPA1
Entrez Gene ID	423327
Full Name	Ral GTPase activating protein catalytic alpha subunit 1
Synonyms	GARNL1
General protein information	Preferred Names Ral GTPase activating protein catalytic alpha subunit 1 Names ral GTPase-activating protein subunit alpha-1 GTPase activating Rap/RanGAP domain-like 1 GTPase-activating Rap/RanGAP domain-like 1 protein Ral GTPase activating protein, alpha subunit 1 (catalytic)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015287373.1	XP_015142859.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_015287380.1	XP_015142866.1	ral GTPase-activating protein subunit alpha-1 isoform X10
XM_015287381.1	XP_015142867.1	ral GTPase-activating protein subunit alpha-1 isoform X11
XM_015287383.1	XP_015142869.1	ral GTPase-activating protein subunit alpha-1 isoform X12
XM_015287374.2	XP_015142860.1	ral GTPase-activating protein subunit alpha-1 isoform X6
XM_025150742.1	XP_025006510.1	ral GTPase-activating protein subunit alpha-1 isoform X9
XM_015287383.2	XP_015142869.1	ral GTPase-activating protein subunit alpha-1 isoform X14
NM_001277451.2	NP_001264380.1	ral GTPase-activating protein subunit alpha-1 isoform 2
NM_001277447.2	NP_001264376.1	ral GTPase-activating protein subunit alpha-1 isoform 1
NM_001277452.1	NP_001264381.1	ral GTPase-activating protein subunit alpha-1 isoform 3
XM_015287379.1	XP_015142865.1	ral GTPase-activating protein subunit alpha-1 isoform X9
XM_015287374.1	XP_015142860.1	ral GTPase-activating protein subunit alpha-1 isoform X5
XM_015287377.1	XP_015142863.1	ral GTPase-activating protein subunit alpha-1 isoform X7
XM_015287378.1	XP_015142864.1	ral GTPase-activating protein subunit alpha-1 isoform X8
XM_025150740.1	XP_025006508.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_015287377.2	XP_015142863.1	ral GTPase-activating protein subunit alpha-1 isoform X8
XM_015287378.2	XP_015142864.1	ral GTPase-activating protein subunit alpha-1 isoform X10
XM_015287379.2	XP_015142865.1	ral GTPase-activating protein subunit alpha-1 isoform X11
XM_015287380.2	XP_015142866.1	ral GTPase-activating protein subunit alpha-1 isoform X12
XM_015287376.1	XP_015142862.1	ral GTPase-activating protein subunit alpha-1 isoform X7
XM_015287372.1	XP_015142858.1	ral GTPase-activating protein subunit alpha-1 isoform X3
XM_015287375.1	XP_015142861.1	ral GTPase-activating protein subunit alpha-1 isoform X6
XM_015287371.2	XP_015142857.1	ral GTPase-activating protein subunit alpha-1 isoform X2
XM_025150741.1	XP_025006509.1	ral GTPase-activating protein subunit alpha-1 isoform X8
XM_015287381.2	XP_015142867.1	ral GTPase-activating protein subunit alpha-1 isoform X13
NM_001277452.2	NP_001264381.1	ral GTPase-activating protein subunit alpha-1 isoform 3
NM_001277454.2	NP_001264383.1	ral GTPase-activating protein subunit alpha-1 isoform 5
NM_001277447.1	NP_001264376.1	ral GTPase-activating protein subunit alpha-1 isoform 1
NM_001277454.1	NP_001264383.1	ral GTPase-activating protein subunit alpha-1 isoform 5
NM_001277453.1	NP_001264382.1	ral GTPase-activating protein subunit alpha-1 isoform 4
XM_015287370.1	XP_015142856.1	ral GTPase-activating protein subunit alpha-1 isoform X1
XM_015287370.2	XP_015142856.1	ral GTPase-activating protein subunit alpha-1 isoform X1
XM_015287372.2	XP_015142858.1	ral GTPase-activating protein subunit alpha-1 isoform X3
XM_015287371.1	XP_015142857.1	ral GTPase-activating protein subunit alpha-1 isoform X2
NM_001277451.1	NP_001264380.1	ral GTPase-activating protein subunit alpha-1 isoform 2
NM_001277453.2	NP_001264382.1	ral GTPase-activating protein subunit alpha-1 isoform 4
XM_015287373.2	XP_015142859.1	ral GTPase-activating protein subunit alpha-1 isoform X5
XM_015287375.2	XP_015142861.1	ral GTPase-activating protein subunit alpha-1 isoform X7
XM_015287376.2	XP_015142862.1	ral GTPase-activating protein subunit alpha-1 isoform X8

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ralgapa1	NP_001003719.1
Homo sapiens (human)	RALGAPA1	NP_001269972.1
Pan troglodytes (chimpanzee)	RALGAPA1	XP_003314394.1
	RALGAPA1	XP_001088633.1
Rattus norvegicus (Norway rat)	Ralgapa1	NP_064468.2
Drosophila melanogaster (fruit fly)	CG5521	NP_001163748.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004143	XP_313024.5
Bos taurus (cattle)	RALGAPA1	XP_005222134.1
Gallus gallus (chicken)	RALGAPA1	NP_001264376.1
Danio rerio (zebrafish)	ralgapa1	NP_001139776.1

Gene Ontology
Bibliography

Related articles in PubMed

The GTPase activating Rap/RanGAP domain-like 1 gene is associated with chicken reproductive traits.
Shen X, Zeng H, Xie L, He J, Li J, Xie X, Luo C, Xu H, Zhou M, Nie Q, Zhang X
PloS one7(4)e33851(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Single nucleotide polymorphisms in GTPase activating Rap/RanGAP domain-like 1 gene is associated with reproductive traits and broodiness.
Title: The GTPase activating Rap/RanGAP domain-like 1 gene is associated with chicken reproductive traits.

The following RALGAPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RALGAPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa23906	NM_001277454.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa150860	XM_025150740.1 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa150861	XM_025150742.1 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa36818	XM_015287372.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36819	XM_015287373.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36820	XM_015287374.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36821	XM_015287375.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36822	XM_025150741.1 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36822	XM_015287376.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36822	XM_015287377.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36823	XM_015287378.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa36824	XM_015287379.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa36825	XM_015287380.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa36826	XM_015287381.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa36827	XM_015287383.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa23826	NM_001277447.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa23873	NM_001277451.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa23887	NM_001277452.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa23898	NM_001277453.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa36816	XM_015287370.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa36817	XM_015287371.2 Latest version!	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OGa36827	XM_015287383.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa36820	XM_015287374.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36822	XM_015287377.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36823	XM_015287378.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa36824	XM_015287379.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa36825	XM_015287380.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa36826	XM_015287381.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa23887	NM_001277452.1	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa23826	NM_001277447.1	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa23906	NM_001277454.1	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa36816	XM_015287370.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa36818	XM_015287372.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36817	XM_015287371.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa23873	NM_001277451.1	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa23898	NM_001277453.1	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa36819	XM_015287373.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36821	XM_015287375.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa36822	XM_015287376.1	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RALGAPA1 ( NM_001277454.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264383.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 5, mRNA.

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CloneID	OGa23906
Clone ID Related Accession (Same CDS sequence)	NM_001277454.2 , NM_001277454.1
Accession Version	NM_001277454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000303.1. On Dec 20, 2018 this sequence version replaced NM_001277454.1. Transcript Variant: This variant (5) lacks three in-frame coding exons in the central region, but has an additional exon in the 3' region, compared to variant 1. The resulting isoform (5) lacks three internal segments and has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGAAA CTGGTGAATT TCCCTCAGAA TGTTGCAGTG TTATGGCTGG GGGAACACTT 
ACAGGATGGC ACGCAGATGT TGCTACTGTG ATGTGGAGAC GAATGCTAGG AATTTTAGGT 
GATGTCAACA GCATCATGGA TCCAGAGATT CATGCCCAGG TTTTTGATTA CCTGTGTGAA 
CTGTGGCAGA ATCTGGCCAA GATAAGAGAC AATCTTGGTA TTTCAACAGA TAACCTCACT 
TCACCATCTC CACCAGTTTT AATCCCACCA CTGAGAATTC TAACACCTTG GCTGTTCAAG 
GCAACAATGC TGACTGATAA ATACAAACAA GGGAAGCTAC ATGCTTATAA ACTCATTTGT 
AAGACAATGA AGCGAAGACA AGATGTATCT CCAAACAAGG ATTTTTTGAC TCATTTCTAT 
AATATAATGC ACTGTGGACT TCTTCATGTT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCTC CTCAGTTCTT TTCACTTAGT TTGCCTGGTG CCACCATGCT TATTATGGAT 
TTCATTGTAG CAGCAGGAAG GGTGGCAGCT TCTTCTTTTC TCAATGCACC AAGAGTTGAA 
GCTCAAGTTC TTTTAGGATC TCTTGTCTGT TTTCCCAATT TATATCATGA ACTACCAGCT 
CTTCACCCAA ACATTCCTGA CATAGCTGTG TCTCAGTTTA CAGATGTTAA GGAGCTCATA 
ATTAAAACTG TGTTAAGTTC AGCCAGAGAG GAGCCATCTG GTCCTGCAAG ATGTGTTGCT 
CTTTGCAGCC TTGGTATTTG GATCTGTGAG GAGCTAGTTC ATGAATCTCA TCATCCTCAG 
ATCAAGGAAG CATTGAATGT GATTTGTGTT TCTTTAAAGT TTCCGAATAA GACAGTAGCT 
CAAGTTGCCT GCAGTATGCT GAATATGCTG ATACATTATG TGCATAGACT TCAAGTGTAT 
CAAGCTGATT CACCTTTAAG AATTATTCAA ATTCTGATAG CTACAATTAC CCATCTGCTA 
CCCGGTACAG AAGCTTCATC CTATGAACAG GACAAGAGGT TGGTAGTTTC CTTACTTCTG 
TGCTTATTGG ATTGGATAAT GGCCTTGCCA TTGAAGACCT TGCTGCAGCC TCTTCACGCT 
ACAGGAGCAG AAAATGATAA GACTGAGAGA TCTGTTCTTA ATTGTATATA TAAGGTTCTA 
CATGGATGTG TCTATGGATC TCAGTGTTTT AGTAACCCCA AATATTTTCC CCTAAGTCTT 
TCTGATTTGG CATGTGTGGA TTACGATCCA TTTATGCATT TAGAAAGCTT GAAGGAGCCA 
GAACCACTGC ATTCTCCAGA CTCAGAGCGT TCTTCTAAAC TTCAACCAGT CACCGAAGTG 
AAAACTCACA TGCAACAAGG ATTAATTTCT GTTGCTGCTC GTACTGTGAT AACGCATCTA 
GTCAACCACT TAGGTCACTA TCCTATGAGT GGAGGACCTG CTATGCTGAC TAGTCAAGTG 
TGTGAAAATA ATGACAATCC ATATAGTGAA AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG 
AACCCAAACC TTCAATTCTT TGTGTTAAAC AATACTTCCT TGGTGTCTTG CATACAAATC 
CAAGCAGAAG ATGACATGCC TGGGGGAGGA CTGTCTGCTG GACTTGCATC TGCTAATTCA 
AATGTCAGAA TTATAGTGCG TGATCTGTCT GGCAAATACT CATGGGATTC AGCCATTCTG 
TATGGACCAT CATCCTTATG TGCATCTTCA CAACAAACAT CTTTTGCCCT TTCATTATCT 
CAACAAGAAA AAACGGAAGA TCCCCTTTCT TCTTTTGAGC ATGTGGAGGA CATATCTGCA 
AGGGATGGAA TTACACTCCA GGTAAAAAGG AAATTCAGAG ACACTGTGCC AACATGGGAT 
ACAATTAGGG ATGAAGAAGA TGCCCTTGAT GAGCTCTTGC AGTATTTAGG TGTTACAAGC 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTCAACATCC CTGCTCCTCA ACCAGTCTGC 
ATCTCAGAGA AGCAGGAGAA TGACGTCATC AATGCAATTC TGAAACAGCA TACAGAAGAG 
AGAGAGTTTG TTGAAAAACA TTTCAATGAT TTAAATATGA GGGCTATGGA GCAGGATGAG 
CCAACATCGC AAAAGCCCCA GTCAGCATTT TACTACTGCA GACTACTTCT CAGTATACTG 
GGAATGAATT CTTGGGATAA AAGAAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAGTTA 
CTTCGAGAGC TCAGAAATTT GGACTCAAGA CAGTGCCGAG AGACCCACAA GATTGCAGTG 
TTTTATGTTG CTGAGGGACA AGAAGATAAA CACTCCATTC TTACTAATAC TGGGGGAAGT 
CAAGCCTATG AGGATTTTGT AGCTGGCCTT GGCTGGGAGG TAAATCTCAC AAACCATTGT 
GGCTTTATGG GAGGACTGCA AAAAAACAAA AGCACTGGAT TGACCACTCC ATATTTTGCT 
ACCTCAACAG TAGAAGTAAT GTTCCATGTG TCAACAAGAA TGCCTTCTGA TTCAGATGAC 
TCTCTAACAA AAAAGCTAAG ACATTTAGGG AACGATGAAG TACACATTGT TTGGTCAGAG 
CATACTCGAG ATTATAGAAG AGGAATAATT CCAACCGAAT TTGGTGATGT TCTTATTGTT 
ATCTATCCCA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAGAAGCC TGAGGTTCCA 
TTTTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAATTCTTCC TATTATGGTT 
CGCGCAACAG CTATAAATGC AAGCCGTGCA TTGAAATCAT TAATCCCTTT ATACCAAAAC 
TTCTATGAGG AGAGAGCACG ATACCTGCAA ACAATTGTTC AGCATCACCT AGAACCTACA 
ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC TGTCCAGCAC CTTACCACCA TTTGCCCTCA 
GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264383.1
CDS	217..6171
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDADH

Target ORF information:

RefSeq Version	NM_001277454.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277454.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGAAA CTGGTGAATT TCCCTCAGAA TGTTGCAGTG TTATGGCTGG GGGAACACTT 
ACAGGATGGC ACGCAGATGT TGCTACTGTG ATGTGGAGAC GAATGCTAGG AATTTTAGGT 
GATGTCAACA GCATCATGGA TCCAGAGATT CATGCCCAGG TTTTTGATTA CCTGTGTGAA 
CTGTGGCAGA ATCTGGCCAA GATAAGAGAC AATCTTGGTA TTTCAACAGA TAACCTCACT 
TCACCATCTC CACCAGTTTT AATCCCACCA CTGAGAATTC TAACACCTTG GCTGTTCAAG 
GCAACAATGC TGACTGATAA ATACAAACAA GGGAAGCTAC ATGCTTATAA ACTCATTTGT 
AAGACAATGA AGCGAAGACA AGATGTATCT CCAAACAAGG ATTTTTTGAC TCATTTCTAT 
AATATAATGC ACTGTGGACT TCTTCATGTT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCTC CTCAGTTCTT TTCACTTAGT TTGCCTGGTG CCACCATGCT TATTATGGAT 
TTCATTGTAG CAGCAGGAAG GGTGGCAGCT TCTTCTTTTC TCAATGCACC AAGAGTTGAA 
GCTCAAGTTC TTTTAGGATC TCTTGTCTGT TTTCCCAATT TATATCATGA ACTACCAGCT 
CTTCACCCAA ACATTCCTGA CATAGCTGTG TCTCAGTTTA CAGATGTTAA GGAGCTCATA 
ATTAAAACTG TGTTAAGTTC AGCCAGAGAG GAGCCATCTG GTCCTGCAAG ATGTGTTGCT 
CTTTGCAGCC TTGGTATTTG GATCTGTGAG GAGCTAGTTC ATGAATCTCA TCATCCTCAG 
ATCAAGGAAG CATTGAATGT GATTTGTGTT TCTTTAAAGT TTCCGAATAA GACAGTAGCT 
CAAGTTGCCT GCAGTATGCT GAATATGCTG ATACATTATG TGCATAGACT TCAAGTGTAT 
CAAGCTGATT CACCTTTAAG AATTATTCAA ATTCTGATAG CTACAATTAC CCATCTGCTA 
CCCGGTACAG AAGCTTCATC CTATGAACAG GACAAGAGGT TGGTAGTTTC CTTACTTCTG 
TGCTTATTGG ATTGGATAAT GGCCTTGCCA TTGAAGACCT TGCTGCAGCC TCTTCACGCT 
ACAGGAGCAG AAAATGATAA GACTGAGAGA TCTGTTCTTA ATTGTATATA TAAGGTTCTA 
CATGGATGTG TCTATGGATC TCAGTGTTTT AGTAACCCCA AATATTTTCC CCTAAGTCTT 
TCTGATTTGG CATGTGTGGA TTACGATCCA TTTATGCATT TAGAAAGCTT GAAGGAGCCA 
GAACCACTGC ATTCTCCAGA CTCAGAGCGT TCTTCTAAAC TTCAACCAGT CACCGAAGTG 
AAAACTCACA TGCAACAAGG ATTAATTTCT GTTGCTGCTC GTACTGTGAT AACGCATCTA 
GTCAACCACT TAGGTCACTA TCCTATGAGT GGAGGACCTG CTATGCTGAC TAGTCAAGTG 
TGTGAAAATA ATGACAATCC ATATAGTGAA AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG 
AACCCAAACC TTCAATTCTT TGTGTTAAAC AATACTTCCT TGGTGTCTTG CATACAAATC 
CAAGCAGAAG ATGACATGCC TGGGGGAGGA CTGTCTGCTG GACTTGCATC TGCTAATTCA 
AATGTCAGAA TTATAGTGCG TGATCTGTCT GGCAAATACT CATGGGATTC AGCCATTCTG 
TATGGACCAT CATCCTTATG TGCATCTTCA CAACAAACAT CTTTTGCCCT TTCATTATCT 
CAACAAGAAA AAACGGAAGA TCCCCTTTCT TCTTTTGAGC ATGTGGAGGA CATATCTGCA 
AGGGATGGAA TTACACTCCA GGTAAAAAGG AAATTCAGAG ACACTGTGCC AACATGGGAT 
ACAATTAGGG ATGAAGAAGA TGCCCTTGAT GAGCTCTTGC AGTATTTAGG TGTTACAAGC 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTCAACATCC CTGCTCCTCA ACCAGTCTGC 
ATCTCAGAGA AGCAGGAGAA TGACGTCATC AATGCAATTC TGAAACAGCA TACAGAAGAG 
AGAGAGTTTG TTGAAAAACA TTTCAATGAT TTAAATATGA GGGCTATGGA GCAGGATGAG 
CCAACATCGC AAAAGCCCCA GTCAGCATTT TACTACTGCA GACTACTTCT CAGTATACTG 
GGAATGAATT CTTGGGATAA AAGAAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAGTTA 
CTTCGAGAGC TCAGAAATTT GGACTCAAGA CAGTGCCGAG AGACCCACAA GATTGCAGTG 
TTTTATGTTG CTGAGGGACA AGAAGATAAA CACTCCATTC TTACTAATAC TGGGGGAAGT 
CAAGCCTATG AGGATTTTGT AGCTGGCCTT GGCTGGGAGG TAAATCTCAC AAACCATTGT 
GGCTTTATGG GAGGACTGCA AAAAAACAAA AGCACTGGAT TGACCACTCC ATATTTTGCT 
ACCTCAACAG TAGAAGTAAT GTTCCATGTG TCAACAAGAA TGCCTTCTGA TTCAGATGAC 
TCTCTAACAA AAAAGCTAAG ACATTTAGGG AACGATGAAG TACACATTGT TTGGTCAGAG 
CATACTCGAG ATTATAGAAG AGGAATAATT CCAACCGAAT TTGGTGATGT TCTTATTGTT 
ATCTATCCCA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAGAAGCC TGAGGTTCCA 
TTTTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAATTCTTCC TATTATGGTT 
CGCGCAACAG CTATAAATGC AAGCCGTGCA TTGAAATCAT TAATCCCTTT ATACCAAAAC 
TTCTATGAGG AGAGAGCACG ATACCTGCAA ACAATTGTTC AGCATCACCT AGAACCTACA 
ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC TGTCCAGCAC CTTACCACCA TTTGCCCTCA 
GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( NM_001277454.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264383.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 5, mRNA.

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CloneID	OGa23906
Clone ID Related Accession (Same CDS sequence)	NM_001277454.2 , NM_001277454.1
Accession Version	NM_001277454.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-05
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04000014.1. Transcript Variant: This variant (5) lacks three in-frame coding exons in the central region, but has an additional exon in the 3' region, compared to variant 1. The resulting isoform (5) lacks three internal segments and has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGAAA CTGGTGAATT TCCCTCAGAA TGTTGCAGTG TTATGGCTGG GGGAACACTT 
ACAGGATGGC ACGCAGATGT TGCTACTGTG ATGTGGAGAC GAATGCTAGG AATTTTAGGT 
GATGTCAACA GCATCATGGA TCCAGAGATT CATGCCCAGG TTTTTGATTA CCTGTGTGAA 
CTGTGGCAGA ATCTGGCCAA GATAAGAGAC AATCTTGGTA TTTCAACAGA TAACCTCACT 
TCACCATCTC CACCAGTTTT AATCCCACCA CTGAGAATTC TAACACCTTG GCTGTTCAAG 
GCAACAATGC TGACTGATAA ATACAAACAA GGGAAGCTAC ATGCTTATAA ACTCATTTGT 
AAGACAATGA AGCGAAGACA AGATGTATCT CCAAACAAGG ATTTTTTGAC TCATTTCTAT 
AATATAATGC ACTGTGGACT TCTTCATGTT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCTC CTCAGTTCTT TTCACTTAGT TTGCCTGGTG CCACCATGCT TATTATGGAT 
TTCATTGTAG CAGCAGGAAG GGTGGCAGCT TCTTCTTTTC TCAATGCACC AAGAGTTGAA 
GCTCAAGTTC TTTTAGGATC TCTTGTCTGT TTTCCCAATT TATATCATGA ACTACCAGCT 
CTTCACCCAA ACATTCCTGA CATAGCTGTG TCTCAGTTTA CAGATGTTAA GGAGCTCATA 
ATTAAAACTG TGTTAAGTTC AGCCAGAGAG GAGCCATCTG GTCCTGCAAG ATGTGTTGCT 
CTTTGCAGCC TTGGTATTTG GATCTGTGAG GAGCTAGTTC ATGAATCTCA TCATCCTCAG 
ATCAAGGAAG CATTGAATGT GATTTGTGTT TCTTTAAAGT TTCCGAATAA GACAGTAGCT 
CAAGTTGCCT GCAGTATGCT GAATATGCTG ATACATTATG TGCATAGACT TCAAGTGTAT 
CAAGCTGATT CACCTTTAAG AATTATTCAA ATTCTGATAG CTACAATTAC CCATCTGCTA 
CCCGGTACAG AAGCTTCATC CTATGAACAG GACAAGAGGT TGGTAGTTTC CTTACTTCTG 
TGCTTATTGG ATTGGATAAT GGCCTTGCCA TTGAAGACCT TGCTGCAGCC TCTTCACGCT 
ACAGGAGCAG AAAATGATAA GACTGAGAGA TCTGTTCTTA ATTGTATATA TAAGGTTCTA 
CATGGATGTG TCTATGGATC TCAGTGTTTT AGTAACCCCA AATATTTTCC CCTAAGTCTT 
TCTGATTTGG CATGTGTGGA TTACGATCCA TTTATGCATT TAGAAAGCTT GAAGGAGCCA 
GAACCACTGC ATTCTCCAGA CTCAGAGCGT TCTTCTAAAC TTCAACCAGT CACCGAAGTG 
AAAACTCACA TGCAACAAGG ATTAATTTCT GTTGCTGCTC GTACTGTGAT AACGCATCTA 
GTCAACCACT TAGGTCACTA TCCTATGAGT GGAGGACCTG CTATGCTGAC TAGTCAAGTG 
TGTGAAAATA ATGACAATCC ATATAGTGAA AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG 
AACCCAAACC TTCAATTCTT TGTGTTAAAC AATACTTCCT TGGTGTCTTG CATACAAATC 
CAAGCAGAAG ATGACATGCC TGGGGGAGGA CTGTCTGCTG GACTTGCATC TGCTAATTCA 
AATGTCAGAA TTATAGTGCG TGATCTGTCT GGCAAATACT CATGGGATTC AGCCATTCTG 
TATGGACCAT CATCCTTATG TGCATCTTCA CAACAAACAT CTTTTGCCCT TTCATTATCT 
CAACAAGAAA AAACGGAAGA TCCCCTTTCT TCTTTTGAGC ATGTGGAGGA CATATCTGCA 
AGGGATGGAA TTACACTCCA GGTAAAAAGG AAATTCAGAG ACACTGTGCC AACATGGGAT 
ACAATTAGGG ATGAAGAAGA TGCCCTTGAT GAGCTCTTGC AGTATTTAGG TGTTACAAGC 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTCAACATCC CTGCTCCTCA ACCAGTCTGC 
ATCTCAGAGA AGCAGGAGAA TGACGTCATC AATGCAATTC TGAAACAGCA TACAGAAGAG 
AGAGAGTTTG TTGAAAAACA TTTCAATGAT TTAAATATGA GGGCTATGGA GCAGGATGAG 
CCAACATCGC AAAAGCCCCA GTCAGCATTT TACTACTGCA GACTACTTCT CAGTATACTG 
GGAATGAATT CTTGGGATAA AAGAAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAGTTA 
CTTCGAGAGC TCAGAAATTT GGACTCAAGA CAGTGCCGAG AGACCCACAA GATTGCAGTG 
TTTTATGTTG CTGAGGGACA AGAAGATAAA CACTCCATTC TTACTAATAC TGGGGGAAGT 
CAAGCCTATG AGGATTTTGT AGCTGGCCTT GGCTGGGAGG TAAATCTCAC AAACCATTGT 
GGCTTTATGG GAGGACTGCA AAAAAACAAA AGCACTGGAT TGACCACTCC ATATTTTGCT 
ACCTCAACAG TAGAAGTAAT GTTCCATGTG TCAACAAGAA TGCCTTCTGA TTCAGATGAC 
TCTCTAACAA AAAAGCTAAG ACATTTAGGG AACGATGAAG TACACATTGT TTGGTCAGAG 
CATACTCGAG ATTATAGAAG AGGAATAATT CCAACCGAAT TTGGTGATGT TCTTATTGTT 
ATCTATCCCA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAGAAGCC TGAGGTTCCA 
TTTTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAATTCTTCC TATTATGGTT 
CGCGCAACAG CTATAAATGC AAGCCGTGCA TTGAAATCAT TAATCCCTTT ATACCAAAAC 
TTCTATGAGG AGAGAGCACG ATACCTGCAA ACAATTGTTC AGCATCACCT AGAACCTACA 
ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC TGTCCAGCAC CTTACCACCA TTTGCCCTCA 
GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264383.1
CDS	217..6171
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDADH

Target ORF information:

RefSeq Version	NM_001277454.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277454.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGAAA CTGGTGAATT TCCCTCAGAA TGTTGCAGTG TTATGGCTGG GGGAACACTT 
ACAGGATGGC ACGCAGATGT TGCTACTGTG ATGTGGAGAC GAATGCTAGG AATTTTAGGT 
GATGTCAACA GCATCATGGA TCCAGAGATT CATGCCCAGG TTTTTGATTA CCTGTGTGAA 
CTGTGGCAGA ATCTGGCCAA GATAAGAGAC AATCTTGGTA TTTCAACAGA TAACCTCACT 
TCACCATCTC CACCAGTTTT AATCCCACCA CTGAGAATTC TAACACCTTG GCTGTTCAAG 
GCAACAATGC TGACTGATAA ATACAAACAA GGGAAGCTAC ATGCTTATAA ACTCATTTGT 
AAGACAATGA AGCGAAGACA AGATGTATCT CCAAACAAGG ATTTTTTGAC TCATTTCTAT 
AATATAATGC ACTGTGGACT TCTTCATGTT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCTC CTCAGTTCTT TTCACTTAGT TTGCCTGGTG CCACCATGCT TATTATGGAT 
TTCATTGTAG CAGCAGGAAG GGTGGCAGCT TCTTCTTTTC TCAATGCACC AAGAGTTGAA 
GCTCAAGTTC TTTTAGGATC TCTTGTCTGT TTTCCCAATT TATATCATGA ACTACCAGCT 
CTTCACCCAA ACATTCCTGA CATAGCTGTG TCTCAGTTTA CAGATGTTAA GGAGCTCATA 
ATTAAAACTG TGTTAAGTTC AGCCAGAGAG GAGCCATCTG GTCCTGCAAG ATGTGTTGCT 
CTTTGCAGCC TTGGTATTTG GATCTGTGAG GAGCTAGTTC ATGAATCTCA TCATCCTCAG 
ATCAAGGAAG CATTGAATGT GATTTGTGTT TCTTTAAAGT TTCCGAATAA GACAGTAGCT 
CAAGTTGCCT GCAGTATGCT GAATATGCTG ATACATTATG TGCATAGACT TCAAGTGTAT 
CAAGCTGATT CACCTTTAAG AATTATTCAA ATTCTGATAG CTACAATTAC CCATCTGCTA 
CCCGGTACAG AAGCTTCATC CTATGAACAG GACAAGAGGT TGGTAGTTTC CTTACTTCTG 
TGCTTATTGG ATTGGATAAT GGCCTTGCCA TTGAAGACCT TGCTGCAGCC TCTTCACGCT 
ACAGGAGCAG AAAATGATAA GACTGAGAGA TCTGTTCTTA ATTGTATATA TAAGGTTCTA 
CATGGATGTG TCTATGGATC TCAGTGTTTT AGTAACCCCA AATATTTTCC CCTAAGTCTT 
TCTGATTTGG CATGTGTGGA TTACGATCCA TTTATGCATT TAGAAAGCTT GAAGGAGCCA 
GAACCACTGC ATTCTCCAGA CTCAGAGCGT TCTTCTAAAC TTCAACCAGT CACCGAAGTG 
AAAACTCACA TGCAACAAGG ATTAATTTCT GTTGCTGCTC GTACTGTGAT AACGCATCTA 
GTCAACCACT TAGGTCACTA TCCTATGAGT GGAGGACCTG CTATGCTGAC TAGTCAAGTG 
TGTGAAAATA ATGACAATCC ATATAGTGAA AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG 
AACCCAAACC TTCAATTCTT TGTGTTAAAC AATACTTCCT TGGTGTCTTG CATACAAATC 
CAAGCAGAAG ATGACATGCC TGGGGGAGGA CTGTCTGCTG GACTTGCATC TGCTAATTCA 
AATGTCAGAA TTATAGTGCG TGATCTGTCT GGCAAATACT CATGGGATTC AGCCATTCTG 
TATGGACCAT CATCCTTATG TGCATCTTCA CAACAAACAT CTTTTGCCCT TTCATTATCT 
CAACAAGAAA AAACGGAAGA TCCCCTTTCT TCTTTTGAGC ATGTGGAGGA CATATCTGCA 
AGGGATGGAA TTACACTCCA GGTAAAAAGG AAATTCAGAG ACACTGTGCC AACATGGGAT 
ACAATTAGGG ATGAAGAAGA TGCCCTTGAT GAGCTCTTGC AGTATTTAGG TGTTACAAGC 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTCAACATCC CTGCTCCTCA ACCAGTCTGC 
ATCTCAGAGA AGCAGGAGAA TGACGTCATC AATGCAATTC TGAAACAGCA TACAGAAGAG 
AGAGAGTTTG TTGAAAAACA TTTCAATGAT TTAAATATGA GGGCTATGGA GCAGGATGAG 
CCAACATCGC AAAAGCCCCA GTCAGCATTT TACTACTGCA GACTACTTCT CAGTATACTG 
GGAATGAATT CTTGGGATAA AAGAAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAGTTA 
CTTCGAGAGC TCAGAAATTT GGACTCAAGA CAGTGCCGAG AGACCCACAA GATTGCAGTG 
TTTTATGTTG CTGAGGGACA AGAAGATAAA CACTCCATTC TTACTAATAC TGGGGGAAGT 
CAAGCCTATG AGGATTTTGT AGCTGGCCTT GGCTGGGAGG TAAATCTCAC AAACCATTGT 
GGCTTTATGG GAGGACTGCA AAAAAACAAA AGCACTGGAT TGACCACTCC ATATTTTGCT 
ACCTCAACAG TAGAAGTAAT GTTCCATGTG TCAACAAGAA TGCCTTCTGA TTCAGATGAC 
TCTCTAACAA AAAAGCTAAG ACATTTAGGG AACGATGAAG TACACATTGT TTGGTCAGAG 
CATACTCGAG ATTATAGAAG AGGAATAATT CCAACCGAAT TTGGTGATGT TCTTATTGTT 
ATCTATCCCA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAGAAGCC TGAGGTTCCA 
TTTTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAATTCTTCC TATTATGGTT 
CGCGCAACAG CTATAAATGC AAGCCGTGCA TTGAAATCAT TAATCCCTTT ATACCAAAAC 
TTCTATGAGG AGAGAGCACG ATACCTGCAA ACAATTGTTC AGCATCACCT AGAACCTACA 
ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC TGTCCAGCAC CTTACCACCA TTTGCCCTCA 
GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_025150740.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006508.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.

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CloneID	OGa150860
Clone ID Related Accession (Same CDS sequence)	XM_025150740.1
Accession Version	XM_025150740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGAAA GAAATGCTGT GATATTTCTT 
CTCCTTCCAA AACCTCCACT AGAAAGCATT GGCCACATCA AGGCTCCTGC CCAGAGACTC 
AGCCGGGAGA GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA GGCGGATGGA ACCGACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006508.1
CDS	269..8248
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKAPAQRLSRESLQQQQQQQRRRMEPT

Target ORF information:

RefSeq Version	XM_025150740.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150740.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGAAA GAAATGCTGT GATATTTCTT 
CTCCTTCCAA AACCTCCACT AGAAAGCATT GGCCACATCA AGGCTCCTGC CCAGAGACTC 
AGCCGGGAGA GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA GGCGGATGGA ACCGACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_025150742.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006510.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X11, mRNA.

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CloneID	OGa150861
Clone ID Related Accession (Same CDS sequence)	XM_025150742.1
Accession Version	XM_025150742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGGT GCAGTTCCTG TCATTGACAG TTCATCCCAT 
CACGCTCCAA GTTTACAGAG TTCCACTGAG ACCTCTTCAA TGCATAGATC CACTGAAAGC 
CATATTACTG ATTCAAATAG CAGAGAGTCC TCCTTGGAAG TTGGGGATAG TATTTATGAC 
CATCTTTGTC ATTTAATAGG GCCAGTAGAA TTTGCAAACA CAACCTTTGA ACAAAACCAG 
TGTGTTGACC TTGAAGGTGA AGATGATCTT CTTTCCTCTC TGAGAGAATA TCTTAAAGAA 
AAAGAAGAAC TTTGCGGTAA CTTTGAGATA GATGAATGTG AGGCTTCTGC TCACGAAGGT 
GATACATCAG TAAATAGGAA TGATAGATTA CCACTGGATG GATTAGAAAA TAGTGATCAG 
ACTGGTCAAT GCACGAATAG CATCACTGTT ACAGAAAGAC CTGATAACTC AGAGCTGCAC 
CTAGAACAAA ACCAAAGTGG CTTTATAAGT AATATTGCAC CTACTCAAAT AGACATTTTT 
GCAAGAAATG AAGCACTCGA TAAAGTCAGA CAGTTAAATA TCGATAAACA GTGTGAAAGT 
CTGTTTGATC ATGGGCCAAT TAGTCCTAGC ATCAAAGAAG GAAAAAGATA CCTGTACAGG 
CAAGCAGCCA CTGAAGCTGA TGTAGATTCA GCCATGGAAG CAGCGTTAGC TGAAAAGCAT 
AAAAGTGCTC AATTTAACGA AAAAATAACC AACTCACGTG TTATGCATGC AAAGAAAATT 
CTTCCTAAAG CACAAGTTAA CCCTCAAATG CCTCACATCG CAGGTGCAAG CAAGCTGTCA 
CCAACTAAAA GGAGTATATC TGAAACAGTA ACTCATAGGG CCAAAATCAT GAAAATAACA 
GCTAAAAAAA GAAATAGCGT TCATGTAACT TTTAGACCAT CTACAGAATC AGTTCAGTTT 
TACAATCCTC TTGAGAACAA AGAGGCTGCT TGGAAAGCAA GACTTCGTAA ACTTAGTGGC 
TTCAGCGGTA GTGGTAGCGG CAACAGCAGT AGCAGTACAA GCACCAGCTC ATCAGCTGCC 
ACGGAGCTGG TTAGACCTGG AATATATAGA CCTCTAGATG CAATCAATGC TGCTTCACTT 
GGTAGCAAGC AAAGTAAGGA AATTACAGAA ATTCAAGCAG CTGTGCTGCA AAAGGAAAGT 
GTTGCAGTTA ATCAGTTCCA TAATCGTAGT GCATTTAGAT CATCAGGTCA GGAGTCAGCG 
CAACAGCAGG GCTCCCTGGG TGGTGTTTAT AAAACTGTTG TACATGCTCT TTCGAAGCCG 
AAGGCAAATG TTTCCCCACA GAGGCAGAAC AGAATGCCAG CAGAGGCTCC ACTGAGGGAT 
CTGTACAGTC ATGTAATGGG CTATTTTGGA AGGAAAGCTG CAGCTAATAG AGAGGACATG 
AGTCAAAAGC TGCACCCTAT TGATAGTGAT ATTGGCAGTA GCAATACAAA TGTTCCTGAC 
CTCATGGATG AATTTATAGC TGAGAGACTC CGCAGTGGTA GTACACTGAA TGTGACAAGA 
CGGGGAAGCA GTCCTGGCAG CCTGGAAGTT CCAAAAGATC TTCCTGATGT ATTGAACAAA 
CAGAACCAAA TGCGTCCTAT AGATGACCCA GGCGTGCCTT CCGAATGGAC ATCACCTGCT 
AGCGCAGGCA GCAGTGACCT CATCAGTTCA GATAGCCACT CGGACTCGTT CAGTGCTTTT 
CAGTATGATG GACGCAAATT TGAAAATTTC AGCTTTGGCA CAGAGGCAGG GACGCCAGCT 
TCCACTGATG CTGATGCAAT TTCAGGACAG CAGCAGAGTG CTGAGGAACA AGAAGTAGCC 
AGTCTAACTA CACTCCACAT AGATTCGGAA ACAAGTAGTC TGAATCAGCA ACCGTTGTCT 
GCTGAAGCTG CAACTGTTAC TGGCTCTGAA AGTGCTTCTC CAATTCAATC TGCTCTTGGA 
TCCCGATCAC AGACACCCTC TCCTGCCACT CTTCATGCAG ATCATATTGA GCAGAAGGAT 
CTGCAACTGG ATGAGAAGCT CCACCACTCT GTTTTGCAGA CGCCAGATGA CCTAGAAACT 
GGTGAATTTC CCTCAGAATG TTGCAGTGTT ATGGCTGGGG GAACACTTAC AGGATGGCAC 
GCAGATGTTG CTACTGTGAT GTGGAGACGA ATGCTAGGAA TTTTAGGTGA TGTCAACAGC 
ATCATGGATC CAGAGATTCA TGCCCAGGTT TTTGATTACC TGTGTGAACT GTGGCAGAAT 
CTGGCCAAGA TAAGAGACAA TCTTGGTATT TCAACAGATA ACCTCACTTC ACCATCTCCA 
CCAGTTTTAA TCCCACCACT GAGAATTCTA ACACCTTGGC TGTTCAAGGC AACAATGCTG 
ACTGATAAAT ACAAACAAGG GAAGCTACAT GCTTATAAAC TCATTTGTAA GACAATGAAG 
CGAAGACAAG ATGTATCTCC AAACAAGGAT TTTTTGACTC ATTTCTATAA TATAATGCAC 
TGTGGACTTC TTCATGTTGA TCAGGATATT GTCAATACAA TCATCAAGCA CTGCTCTCCT 
CAGTTCTTTT CACTTAGTTT GCCTGGTGCC ACCATGCTTA TTATGGATTT CATTGTAGCA 
GCAGGAAGGG TGGCAGCTTC TTCTTTTCTC AATGCACCAA GAGTTGAAGC TCAAGTTCTT 
TTAGGATCTC TTGTCTGTTT TCCCAATTTA TATCATGAAC TACCAGCTCT TCACCCAAAC 
ATTCCTGACA TAGCTGTGTC TCAGTTTACA GATGTTAAGG AGCTCATAAT TAAAACTGTG 
TTAAGTTCAG CCAGAGAGGA GCCATCTGGT CCTGCAAGAT GTGTTGCTCT TTGCAGCCTT 
GGTATTTGGA TCTGTGAGGA GCTAGTTCAT GAATCTCATC ATCCTCAGAT CAAGGAAGCA 
TTGAATGTGA TTTGTGTTTC TTTAAAGTTT CCGAATAAGA CAGTAGCTCA AGTTGCCTGC 
AGTATGCTGA ATATGCTGAT ACATTATGTG CATAGACTTC AAGTGTATCA AGCTGATTCA 
CCTTTAAGAA TTATTCAAAT TCTGATAGCT ACAATTACCC ATCTGCTACC CGGTACAGAA 
GCTTCATCCT ATGAACAGGA CAAGAGGTTG GTAGTTTCCT TACTTCTGTG CTTATTGGAT 
TGGATAATGG CCTTGCCATT GAAGACCTTG CTGCAGCCTC TTCACGCTAC AGGAGCAGAA 
AATGATAAGA CTGAGAGATC TGTTCTTAAT TGTATATATA AGGTTCTACA TGGATGTGTC 
TATGGATCTC AGTGTTTTAG TAACCCCAAA TATTTTCCCC TAAGTCTTTC TGATTTGGCA 
TGTGTGGATT ACGATCCATT TATGCATTTA GAAAGCTTGA AGGAGCCAGA ACCACTGCAT 
TCTCCAGACT CAGAGCGTTC TTCTAAACTT CAACCAGTCA CCGAAGTGAA AACTCACATG 
CAACAAGGAT TAATTTCTGT TGCTGCTCGT ACTGTGATAA CGCATCTAGT CAACCACTTA 
GGTCACTATC CTATGAGTGG AGGACCTGCT ATGCTGACTA GTCAAGTGTG TGAAAATAAT 
GACAATCCAT ATAGTGAAAG TCCTGAACTT TCTCCTGAAC TTTTTGAGAA CCCAAACCTT 
CAATTCTTTG TGTTAAACAA TACTTCCTTG GTGTCTTGCA TACAAATCCA AGCAGAAGAT 
GACATGCCTG GGGGAGGACT GTCTGCTGGA CTTGCATCTG CTAATTCAAA TGTCAGAATT 
ATAGTGCGTG ATCTGTCTGG CAAATACTCA TGGGATTCAG CCATTCTGTA TGGACCATCA 
TCCTTATGTG CATCTTCACA ACAAACATCT TTTGCCCTTT CATTATCTCA ACAAGAAAAA 
ACGGAAGATC CCCTTTCTTC TTTTGAGCAT GTGGAGGACA TATCTGCAAG GGATGGAATT 
ACACTCCAGG TAAAAAGGAA ATTCAGAGAC ACTGTGCCAA CATGGGATAC AATTAGGGAT 
GAAGAAGATG CCCTTGATGA GCTCTTGCAG TATTTAGGTG TTACAAGCCC TGAATGCTTA 
CAGAGAACTG GAGTCTCACT CAACATCCCT GCTCCTCAAC CAGTCTGCAT CTCAGAGAAG 
CAGGAGAATG ACGTCATCAA TGCAATTCTG AAACAGCATA CAGAAGAGAG AGAGTTTGTT 
GAAAAACATT TCAATGATTT AAATATGAGG GCTATGGAGC AGGATGAGCC AACATCGCAA 
AAGCCCCAGT CAGCATTTTA CTACTGCAGA CTACTTCTCA GTATACTGGG AATGAATTCT 
TGGGATAAAA GAAGGAGCTT TCATCTCCTG AAGAAAAATG AAAAGTTACT TCGAGAGCTC 
AGAAATTTGG ACTCAAGACA GTGCCGAGAG ACCCACAAGA TTGCAGTGTT TTATGTTGCT 
GAGGGACAAG AAGATAAACA CTCCATTCTT ACTAATACTG GGGGAAGTCA AGCCTATGAG 
GATTTTGTAG CTGGCCTTGG CTGGGAGGTA AATCTCACAA ACCATTGTGG CTTTATGGGA 
GGACTGCAAA AAAACAAAAG CACTGGATTG ACCACTCCAT ATTTTGCTAC CTCAACAGTA 
GAAGTAATGT TCCATGTGTC AACAAGAATG CCTTCTGATT CAGATGACTC TCTAACAAAA 
AAGCTAAGAC ATTTAGGGAA CGATGAAGTA CACATTGTTT GGTCAGAGCA TACTCGAGAT 
TATAGAAGAG GAATAATTCC AACCGAATTT GGTGATGTTC TTATTGTTAT CTATCCCATG 
AAAAACCACA TGTTCAGTAT CCAGATCATG AAGAAGCCTG AGGTTCCATT TTTTGGTCCA 
CTTTTTGATG GTGCTATTGT GAATGGAAAA ATTCTTCCTA TTATGGTTCG CGCAACAGCT 
ATAAATGCAA GCCGTGCATT GAAATCATTA ATCCCTTTAT ACCAAAACTT CTATGAGGAG 
AGAGCACGAT ACCTGCAAAC AATTGTTCAG CATCACCTAG AACCTACAAC TTTTGAGGAT 
TTTGCTGCTC AGGTTTTCTG TCCAGCACCT TACCACCATT TGCCCTCAGA TGCAGAAAGA 
AATGCTGTGA TATTTCTTCT CCTTCCAAAA CCTCCACTAG AAAGCATTGG CCACATCAAG 
GTAAAGGGCA AAGGCTTGAA ACGGAAGGTC TTTCACGCTA GCTGTGAATG GCTCCTGCCC 
AGAGACTCAG CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC 
CGACTTAGCC TCACCCATGT CTCCTCGGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006510.1
CDS	1..7713
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_025150742.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150742.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGGT GCAGTTCCTG TCATTGACAG TTCATCCCAT 
CACGCTCCAA GTTTACAGAG TTCCACTGAG ACCTCTTCAA TGCATAGATC CACTGAAAGC 
CATATTACTG ATTCAAATAG CAGAGAGTCC TCCTTGGAAG TTGGGGATAG TATTTATGAC 
CATCTTTGTC ATTTAATAGG GCCAGTAGAA TTTGCAAACA CAACCTTTGA ACAAAACCAG 
TGTGTTGACC TTGAAGGTGA AGATGATCTT CTTTCCTCTC TGAGAGAATA TCTTAAAGAA 
AAAGAAGAAC TTTGCGGTAA CTTTGAGATA GATGAATGTG AGGCTTCTGC TCACGAAGGT 
GATACATCAG TAAATAGGAA TGATAGATTA CCACTGGATG GATTAGAAAA TAGTGATCAG 
ACTGGTCAAT GCACGAATAG CATCACTGTT ACAGAAAGAC CTGATAACTC AGAGCTGCAC 
CTAGAACAAA ACCAAAGTGG CTTTATAAGT AATATTGCAC CTACTCAAAT AGACATTTTT 
GCAAGAAATG AAGCACTCGA TAAAGTCAGA CAGTTAAATA TCGATAAACA GTGTGAAAGT 
CTGTTTGATC ATGGGCCAAT TAGTCCTAGC ATCAAAGAAG GAAAAAGATA CCTGTACAGG 
CAAGCAGCCA CTGAAGCTGA TGTAGATTCA GCCATGGAAG CAGCGTTAGC TGAAAAGCAT 
AAAAGTGCTC AATTTAACGA AAAAATAACC AACTCACGTG TTATGCATGC AAAGAAAATT 
CTTCCTAAAG CACAAGTTAA CCCTCAAATG CCTCACATCG CAGGTGCAAG CAAGCTGTCA 
CCAACTAAAA GGAGTATATC TGAAACAGTA ACTCATAGGG CCAAAATCAT GAAAATAACA 
GCTAAAAAAA GAAATAGCGT TCATGTAACT TTTAGACCAT CTACAGAATC AGTTCAGTTT 
TACAATCCTC TTGAGAACAA AGAGGCTGCT TGGAAAGCAA GACTTCGTAA ACTTAGTGGC 
TTCAGCGGTA GTGGTAGCGG CAACAGCAGT AGCAGTACAA GCACCAGCTC ATCAGCTGCC 
ACGGAGCTGG TTAGACCTGG AATATATAGA CCTCTAGATG CAATCAATGC TGCTTCACTT 
GGTAGCAAGC AAAGTAAGGA AATTACAGAA ATTCAAGCAG CTGTGCTGCA AAAGGAAAGT 
GTTGCAGTTA ATCAGTTCCA TAATCGTAGT GCATTTAGAT CATCAGGTCA GGAGTCAGCG 
CAACAGCAGG GCTCCCTGGG TGGTGTTTAT AAAACTGTTG TACATGCTCT TTCGAAGCCG 
AAGGCAAATG TTTCCCCACA GAGGCAGAAC AGAATGCCAG CAGAGGCTCC ACTGAGGGAT 
CTGTACAGTC ATGTAATGGG CTATTTTGGA AGGAAAGCTG CAGCTAATAG AGAGGACATG 
AGTCAAAAGC TGCACCCTAT TGATAGTGAT ATTGGCAGTA GCAATACAAA TGTTCCTGAC 
CTCATGGATG AATTTATAGC TGAGAGACTC CGCAGTGGTA GTACACTGAA TGTGACAAGA 
CGGGGAAGCA GTCCTGGCAG CCTGGAAGTT CCAAAAGATC TTCCTGATGT ATTGAACAAA 
CAGAACCAAA TGCGTCCTAT AGATGACCCA GGCGTGCCTT CCGAATGGAC ATCACCTGCT 
AGCGCAGGCA GCAGTGACCT CATCAGTTCA GATAGCCACT CGGACTCGTT CAGTGCTTTT 
CAGTATGATG GACGCAAATT TGAAAATTTC AGCTTTGGCA CAGAGGCAGG GACGCCAGCT 
TCCACTGATG CTGATGCAAT TTCAGGACAG CAGCAGAGTG CTGAGGAACA AGAAGTAGCC 
AGTCTAACTA CACTCCACAT AGATTCGGAA ACAAGTAGTC TGAATCAGCA ACCGTTGTCT 
GCTGAAGCTG CAACTGTTAC TGGCTCTGAA AGTGCTTCTC CAATTCAATC TGCTCTTGGA 
TCCCGATCAC AGACACCCTC TCCTGCCACT CTTCATGCAG ATCATATTGA GCAGAAGGAT 
CTGCAACTGG ATGAGAAGCT CCACCACTCT GTTTTGCAGA CGCCAGATGA CCTAGAAACT 
GGTGAATTTC CCTCAGAATG TTGCAGTGTT ATGGCTGGGG GAACACTTAC AGGATGGCAC 
GCAGATGTTG CTACTGTGAT GTGGAGACGA ATGCTAGGAA TTTTAGGTGA TGTCAACAGC 
ATCATGGATC CAGAGATTCA TGCCCAGGTT TTTGATTACC TGTGTGAACT GTGGCAGAAT 
CTGGCCAAGA TAAGAGACAA TCTTGGTATT TCAACAGATA ACCTCACTTC ACCATCTCCA 
CCAGTTTTAA TCCCACCACT GAGAATTCTA ACACCTTGGC TGTTCAAGGC AACAATGCTG 
ACTGATAAAT ACAAACAAGG GAAGCTACAT GCTTATAAAC TCATTTGTAA GACAATGAAG 
CGAAGACAAG ATGTATCTCC AAACAAGGAT TTTTTGACTC ATTTCTATAA TATAATGCAC 
TGTGGACTTC TTCATGTTGA TCAGGATATT GTCAATACAA TCATCAAGCA CTGCTCTCCT 
CAGTTCTTTT CACTTAGTTT GCCTGGTGCC ACCATGCTTA TTATGGATTT CATTGTAGCA 
GCAGGAAGGG TGGCAGCTTC TTCTTTTCTC AATGCACCAA GAGTTGAAGC TCAAGTTCTT 
TTAGGATCTC TTGTCTGTTT TCCCAATTTA TATCATGAAC TACCAGCTCT TCACCCAAAC 
ATTCCTGACA TAGCTGTGTC TCAGTTTACA GATGTTAAGG AGCTCATAAT TAAAACTGTG 
TTAAGTTCAG CCAGAGAGGA GCCATCTGGT CCTGCAAGAT GTGTTGCTCT TTGCAGCCTT 
GGTATTTGGA TCTGTGAGGA GCTAGTTCAT GAATCTCATC ATCCTCAGAT CAAGGAAGCA 
TTGAATGTGA TTTGTGTTTC TTTAAAGTTT CCGAATAAGA CAGTAGCTCA AGTTGCCTGC 
AGTATGCTGA ATATGCTGAT ACATTATGTG CATAGACTTC AAGTGTATCA AGCTGATTCA 
CCTTTAAGAA TTATTCAAAT TCTGATAGCT ACAATTACCC ATCTGCTACC CGGTACAGAA 
GCTTCATCCT ATGAACAGGA CAAGAGGTTG GTAGTTTCCT TACTTCTGTG CTTATTGGAT 
TGGATAATGG CCTTGCCATT GAAGACCTTG CTGCAGCCTC TTCACGCTAC AGGAGCAGAA 
AATGATAAGA CTGAGAGATC TGTTCTTAAT TGTATATATA AGGTTCTACA TGGATGTGTC 
TATGGATCTC AGTGTTTTAG TAACCCCAAA TATTTTCCCC TAAGTCTTTC TGATTTGGCA 
TGTGTGGATT ACGATCCATT TATGCATTTA GAAAGCTTGA AGGAGCCAGA ACCACTGCAT 
TCTCCAGACT CAGAGCGTTC TTCTAAACTT CAACCAGTCA CCGAAGTGAA AACTCACATG 
CAACAAGGAT TAATTTCTGT TGCTGCTCGT ACTGTGATAA CGCATCTAGT CAACCACTTA 
GGTCACTATC CTATGAGTGG AGGACCTGCT ATGCTGACTA GTCAAGTGTG TGAAAATAAT 
GACAATCCAT ATAGTGAAAG TCCTGAACTT TCTCCTGAAC TTTTTGAGAA CCCAAACCTT 
CAATTCTTTG TGTTAAACAA TACTTCCTTG GTGTCTTGCA TACAAATCCA AGCAGAAGAT 
GACATGCCTG GGGGAGGACT GTCTGCTGGA CTTGCATCTG CTAATTCAAA TGTCAGAATT 
ATAGTGCGTG ATCTGTCTGG CAAATACTCA TGGGATTCAG CCATTCTGTA TGGACCATCA 
TCCTTATGTG CATCTTCACA ACAAACATCT TTTGCCCTTT CATTATCTCA ACAAGAAAAA 
ACGGAAGATC CCCTTTCTTC TTTTGAGCAT GTGGAGGACA TATCTGCAAG GGATGGAATT 
ACACTCCAGG TAAAAAGGAA ATTCAGAGAC ACTGTGCCAA CATGGGATAC AATTAGGGAT 
GAAGAAGATG CCCTTGATGA GCTCTTGCAG TATTTAGGTG TTACAAGCCC TGAATGCTTA 
CAGAGAACTG GAGTCTCACT CAACATCCCT GCTCCTCAAC CAGTCTGCAT CTCAGAGAAG 
CAGGAGAATG ACGTCATCAA TGCAATTCTG AAACAGCATA CAGAAGAGAG AGAGTTTGTT 
GAAAAACATT TCAATGATTT AAATATGAGG GCTATGGAGC AGGATGAGCC AACATCGCAA 
AAGCCCCAGT CAGCATTTTA CTACTGCAGA CTACTTCTCA GTATACTGGG AATGAATTCT 
TGGGATAAAA GAAGGAGCTT TCATCTCCTG AAGAAAAATG AAAAGTTACT TCGAGAGCTC 
AGAAATTTGG ACTCAAGACA GTGCCGAGAG ACCCACAAGA TTGCAGTGTT TTATGTTGCT 
GAGGGACAAG AAGATAAACA CTCCATTCTT ACTAATACTG GGGGAAGTCA AGCCTATGAG 
GATTTTGTAG CTGGCCTTGG CTGGGAGGTA AATCTCACAA ACCATTGTGG CTTTATGGGA 
GGACTGCAAA AAAACAAAAG CACTGGATTG ACCACTCCAT ATTTTGCTAC CTCAACAGTA 
GAAGTAATGT TCCATGTGTC AACAAGAATG CCTTCTGATT CAGATGACTC TCTAACAAAA 
AAGCTAAGAC ATTTAGGGAA CGATGAAGTA CACATTGTTT GGTCAGAGCA TACTCGAGAT 
TATAGAAGAG GAATAATTCC AACCGAATTT GGTGATGTTC TTATTGTTAT CTATCCCATG 
AAAAACCACA TGTTCAGTAT CCAGATCATG AAGAAGCCTG AGGTTCCATT TTTTGGTCCA 
CTTTTTGATG GTGCTATTGT GAATGGAAAA ATTCTTCCTA TTATGGTTCG CGCAACAGCT 
ATAAATGCAA GCCGTGCATT GAAATCATTA ATCCCTTTAT ACCAAAACTT CTATGAGGAG 
AGAGCACGAT ACCTGCAAAC AATTGTTCAG CATCACCTAG AACCTACAAC TTTTGAGGAT 
TTTGCTGCTC AGGTTTTCTG TCCAGCACCT TACCACCATT TGCCCTCAGA TGCAGAAAGA 
AATGCTGTGA TATTTCTTCT CCTTCCAAAA CCTCCACTAG AAAGCATTGG CCACATCAAG 
GTAAAGGGCA AAGGCTTGAA ACGGAAGGTC TTTCACGCTA GCTGTGAATG GCTCCTGCCC 
AGAGACTCAG CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC 
CGACTTAGCC TCACCCATGT CTCCTCGGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287372.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142858.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.

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CloneID	OGa36818
Clone ID Related Accession (Same CDS sequence)	XM_015287372.1 , XM_015287372.2
Accession Version	XM_015287372.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGGCT CCTGCCCAGA GACTCAGCCG 
GGAGAGCCTC CAGCAGCAGC AGCAGCAGCA GCGCAGGCGG ATGGAACCGA CTTAGCCTCA 
CCCATGTCTC CTCGGACTAG CAAGAGCCGC ATGTCCATGA AGCTGCGGCG CTCCTCGGGC 
TCGGCCAACA AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142858.1
CDS	270..8267
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015287372.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287372.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGGCT CCTGCCCAGA GACTCAGCCG 
GGAGAGCCTC CAGCAGCAGC AGCAGCAGCA GCGCAGGCGG ATGGAACCGA CTTAGCCTCA 
CCCATGTCTC CTCGGACTAG CAAGAGCCGC ATGTCCATGA AGCTGCGGCG CTCCTCGGGC 
TCGGCCAACA AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287372.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142858.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.

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CloneID	OGa36818
Clone ID Related Accession (Same CDS sequence)	XM_015287372.1 , XM_015287372.2
Accession Version	XM_015287372.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGGCT CCTGCCCAGA GACTCAGCCG 
GGAGAGCCTC CAGCAGCAGC AGCAGCAGCA GCGCAGGCGG ATGGAACCGA CTTAGCCTCA 
CCCATGTCTC CTCGGACTAG CAAGAGCCGC ATGTCCATGA AGCTGCGGCG CTCCTCGGGC 
TCGGCCAACA AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142858.1
CDS	268..8265
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015287372.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287372.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGGCT CCTGCCCAGA GACTCAGCCG 
GGAGAGCCTC CAGCAGCAGC AGCAGCAGCA GCGCAGGCGG ATGGAACCGA CTTAGCCTCA 
CCCATGTCTC CTCGGACTAG CAAGAGCCGC ATGTCCATGA AGCTGCGGCG CTCCTCGGGC 
TCGGCCAACA AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287373.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142859.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.

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CloneID	OGa36819
Clone ID Related Accession (Same CDS sequence)	XM_015287373.1 , XM_015287373.2
Accession Version	XM_015287373.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGGT GCAGTTCCTG TCATTGACAG TTCATCCCAT 
CACGCTCCAA GTTTACAGAG TTCCACTGAG ACCTCTTCAA TGCATAGATC CACTGAAAGC 
CATATTACTG ATTCAAATAG CAGAGAGTCC TCCTTGGAAG TTGGGGATAG TATTTATGAC 
CATCTTTGTC ATTTAATAGG GCCAGTAGAA TTTGCAAACA CAACCTTTGA ACAAAACCAG 
TGTGTTGACC TTGAAGGTGA AGATGATCTT CTTTCCTCTC TGAGAGAATA TCTTAAAGAA 
AAAGAAGAAC TTTGCGGTAA CTTTGAGATA GATGAATGTG AGGCTTCTGC TCACGAAGGT 
GATACATCAG TAAATAGGAA TGATAGATTA CCACTGGATG GATTAGAAAA TAGTGATCAG 
ACTGGTCAAT GCACGAATAG CATCACTGTT ACAGAAAGAC CTGATAACTC AGAGCTGCAC 
CTAGAACAAA ACCAAAGTGG CTTTATAAGT AATATTGCAC CTACTCAAAT AGACATTTTT 
GCAAGAAATG AAGCACTCGA TAAAGTCAGA CAGTTAAATA TCGATAAACA GTGTGAAAGT 
CTGTTTGATC ATGGGCCAAT TAGTCCTAGC ATCAAAGAAG GAAAAAGATA CCTGTACAGG 
CAAGCAGCCA CTGAAGCTGA TGTAGATTCA GCCATGGAAG CAGCGTTAGC TGAAAAGCAT 
AAAAGTGCTC AATTTAACGA AAAAATAACC AACTCACGTG TTATGCATGC AAAGAAAATT 
CTTCCTAAAG CACAAGTTAA CCCTCAAATG CCTCACATCG CAGGTGCAAG CAAGCTGTCA 
CCAACTAAAA GGAGTATATC TGAAACAGTA ACTCATAGGG CCAAAATCAT GAAAATAACA 
GCTAAAAAAA GAAATAGCGT TCATGTAACT TTTAGACCAT CTACAGAATC AGTTCAGTTT 
TACAATCCTC TTGAGAACAA AGAGGCTGCT TGGAAAGCAA GACTTCGTAA ACTTAGTGGC 
TTCAGCGGTA GTGGTAGCGG CAACAGCAGT AGCAGTACAA GCACCAGCTC ATCAGCTGCC 
ACGGAGCTGG TTAGACCTGG AATATATAGA CCTCTAGATG CAATCAATGC TGCTTCACTT 
GGTAGCAAGC AAAGTAAGGA AATTACAGAA ATTCAAGCAG CTGTGCTGCA AAAGGAAAGT 
GTTGCAGTTA ATCAGTTCCA TAATCGTAGT GCATTTAGAT CATCAGGTCA GGAGTCAGCG 
CAACAGCAGG GCTCCCTGGG TGGTGTTTAT AAAACTGTTG TACATGCTCT TTCGAAGCCG 
AAGGCAAATG TTTCCCCACA GAGGCAGAAC AGAATGCCAG CAGAGGCTCC ACTGAGGGAT 
CTGTACAGTC ATGTAATGGG CTATTTTGGA AGGAAAGCTG CAGCTAATAG AGAGGACATG 
AGTCAAAAGC TGCACCCTAT TGATAGTGAT ATTGGCAGTA GCAATACAAA TGTTCCTGAC 
CTCATGGATG AATTTATAGC TGAGAGACTC CGCAGTGGTA GTACACTGAA TGTGACAAGA 
CGGGGAAGCA GTCCTGGCAG CCTGGAAGTT CCAAAAGATC TTCCTGATGT ATTGAACAAA 
CAGAACCAAA TGCGTCCTAT AGATGACCCA GGCGTGCCTT CCGAATGGAC ATCACCTGCT 
AGCGCAGGCA GCAGTGACCT CATCAGTTCA GATAGCCACT CGGACTCGTT CAGTGCTTTT 
CAGTATGATG GACGCAAATT TGAAAGTACA CTTACAGCGA CTAAGCAGAA TACAGGACAT 
GCAATAGGAA TGTCTTCTCC AGAAGTTCAG TCGCAGTTTT GTGACAGAAA TGAGAATGTC 
CATAATAGCC AATTACTTTC TGCTTTTACA TCTTATGTAA ATTGTGCAAC AGTGTGTTGG 
AAGCATAGGA GATGTACATA CAAGTTTTAC ATGGAGGATT TTGGAGATTT CAGCTTTGGC 
ACAGAGGCAG GGACGCCAGC TTCCACTGAT GCTGATGCAA TTTCAGGACA GCAGCAGAGT 
GCTGAGGAAC AAGAAGTAGC CAGTCTAACT ACACTCCACA TAGATTCGGA AACAAGTAGT 
CTGAATCAGC AACCGTTGTC TGCTGAAGCT GCAACTGTTA CTGGCTCTGA AAGTGCTTCT 
CCAATTCAAT CTGCTCTTGG ATCCCGATCA CAGACACCCT CTCCTGCCAC TCTTCATGCA 
GATCATATTG AGCAGAAGGA TCTGCAACTG GATGAGAAGC TCCACCACTC TGTTTTGCAG 
ACGCCAGATG ACCTAGAAAC TGGTGAATTT CCCTCAGAAT GTTGCAGTGT TATGGCTGGG 
GGAACACTTA CAGGATGGCA CGCAGATGTT GCTACTGTGA TGTGGAGACG AATGCTAGGA 
ATTTTAGGTG ATGTCAACAG CATCATGGAT CCAGAGATTC ATGCCCAGGT TTTTGATTAC 
CTGTGTGAAC TGTGGCAGAA TCTGGCCAAG ATAAGAGACA ATCTTGGTAT TTCAACAGAT 
AACCTCACTT CACCATCTCC ACCAGTTTTA ATCCCACCAC TGAGAATTCT AACACCTTGG 
CTGTTCAAGG CAACAATGCT GACTGATAAA TACAAACAAG GGAAGCTACA TGCTTATAAA 
CTCATTTGTA AGACAATGAA GCGAAGACAA GATGTATCTC CAAACAAGGA TTTTTTGACT 
CATTTCTATA ATATAATGCA CTGTGGACTT CTTCATGTTG ATCAGGATAT TGTCAATACA 
ATCATCAAGC ACTGCTCTCC TCAGTTCTTT TCACTTAGTT TGCCTGGTGC CACCATGCTT 
ATTATGGATT TCATTGTAGC AGCAGGAAGG GTGGCAGCTT CTTCTTTTCT CAATGCACCA 
AGAGTTGAAG CTCAAGTTCT TTTAGGATCT CTTGTCTGTT TTCCCAATTT ATATCATGAA 
CTACCAGCTC TTCACCCAAA CATTCCTGAC ATAGCTGTGT CTCAGTTTAC AGATGTTAAG 
GAGCTCATAA TTAAAACTGT GTTAAGTTCA GCCAGAGAGG AGCCATCTGG TCCTGCAAGA 
TGTGTTGCTC TTTGCAGCCT TGGTATTTGG ATCTGTGAGG AGCTAGTTCA TGAATCTCAT 
CATCCTCAGA TCAAGGAAGC ATTGAATGTG ATTTGTGTTT CTTTAAAGTT TCCGAATAAG 
ACAGTAGCTC AAGTTGCCTG CAGTATGCTG AATATGCTGA TACATTATGT GCATAGACTT 
CAAGTGTATC AAGCTGATTC ACCTTTAAGA ATTATTCAAA TTCTGATAGC TACAATTACC 
CATCTGCTAC CCGGTACAGA AGCTTCATCC TATGAACAGG ACAAGAGGTT GGTAGTTTCC 
TTACTTCTGT GCTTATTGGA TTGGATAATG GCCTTGCCAT TGAAGACCTT GCTGCAGCCT 
CTTCACGCTA CAGGAGCAGA AAATGATAAG ACTGAGAGAT CTGTTCTTAA TTGTATATAT 
AAGGTTCTAC ATGGATGTGT CTATGGATCT CAGTGTTTTA GTAACCCCAA ATATTTTCCC 
CTAAGTCTTT CTGATTTGGC ATGTGTGGAT TACGATCCAT TTATGCATTT AGAAAGCTTG 
AAGGAGCCAG AACCACTGCA TTCTCCAGAC TCAGAGCGTT CTTCTAAACT TCAACCAGTC 
ACCGAAGTGA AAACTCACAT GCAACAAGGA TTAATTTCTG TTGCTGCTCG TACTGTGATA 
ACGCATCTAG TCAACCACTT AGGTCACTAT CCTATGAGTG GAGGACCTGC TATGCTGACT 
AGTCAAGTGT GTGAAAATAA TGACAATCCA TATAGTGAAA GTCCTGAACT TTCTCCTGAA 
CTTTTTGAGA ACCCAAACCT TCAATTCTTT GTGTTAAACA ATACTTCCTT GGTGTCTTGC 
ATACAAATCC AAGCAGAAGA TGACATGCCT GGGGGAGGAC TGTCTGCTGG ACTTGCATCT 
GCTAATTCAA ATGTCAGAAT TATAGTGCGT GATCTGTCTG GCAAATACTC ATGGGATTCA 
GCCATTCTGT ATGGACCATC ATCCTTATGT GCATCTTCAC AACAAACATC TTTTGCCCTT 
TCATTATCTC AACAAGAAAA AACGGAAGAT CCCCTTTCTT CTTTTGAGCA TGTGGAGGAC 
ATATCTGCAA GGGATGGAAT TACACTCCAG GTAAAAAGGA AATTCAGAGA CACTGTGCCA 
ACATGGGATA CAATTAGGGA TGAAGAAGAT GCCCTTGATG AGCTCTTGCA GTATTTAGGT 
GTTACAAGCC CTGAATGCTT ACAGAGAACT GGAGTCTCAC TCAACATCCC TGCTCCTCAA 
CCAGTCTGCA TCTCAGAGAA GCAGGAGAAT GACGTCATCA ATGCAATTCT GAAACAGCAT 
ACAGAAGAGA GAGAGTTTGT TGAAAAACAT TTCAATGATT TAAATATGAG GGCTATGGAG 
CAGGATGAGC CAACATCGCA AAAGCCCCAG TCAGCATTTT ACTACTGCAG ACTACTTCTC 
AGTATACTGG GAATGAATTC TTGGGATAAA AGAAGGAGCT TTCATCTCCT GAAGAAAAAT 
GAAAAGTTAC TTCGAGAGCT CAGAAATTTG GACTCAAGAC AGTGCCGAGA GACCCACAAG 
ATTGCAGTGT TTTATGTTGC TGAGGGACAA GAAGATAAAC ACTCCATTCT TACTAATACT 
GGGGGAAGTC AAGCCTATGA GGATTTTGTA GCTGGCCTTG GCTGGGAGGT AAATCTCACA 
AACCATTGTG GCTTTATGGG AGGACTGCAA AAAAACAAAA GCACTGGATT GACCACTCCA 
TATTTTGCTA CCTCAACAGT AGAAGTAATG TTCCATGTGT CAACAAGAAT GCCTTCTGAT 
TCAGATGACT CTCTAACAAA AAAGCTAAGA CATTTAGGGA ACGATGAAGT ACACATTGTT 
TGGTCAGAGC ATACTCGAGA TTATAGAAGA GGAATAATTC CAACCGAATT TGGTGATGTT 
CTTATTGTTA TCTATCCCAT GAAAAACCAC ATGTTCAGTA TCCAGATCAT GAAGAAGCCT 
GAGGTTCCAT TTTTTGGTCC ACTTTTTGAT GGTGCTATTG TGAATGGAAA AATTCTTCCT 
ATTATGGTTC GCGCAACAGC TATAAATGCA AGCCGTGCAT TGAAATCATT AATCCCTTTA 
TACCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTTCA GCATCACCTA 
GAACCTACAA CTTTTGAGGA TTTTGCTGCT CAGGTTTTCT GTCCAGCACC TTACCACCAT 
TTGCCCTCAG ATGCAGAAAG AAATGCTGTG ATATTTCTTC TCCTTCCAAA ACCTCCACTA 
GAAAGCATTG GCCACATCAA GGTAAAGGGC AAAGGCTTGA AACGGAAGGT CTTTCACGCT 
AGCTGTGAAT GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC 
AGCAGCGCAG GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142859.1
CDS	270..8183
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287373.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287373.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGGT GCAGTTCCTG TCATTGACAG TTCATCCCAT 
CACGCTCCAA GTTTACAGAG TTCCACTGAG ACCTCTTCAA TGCATAGATC CACTGAAAGC 
CATATTACTG ATTCAAATAG CAGAGAGTCC TCCTTGGAAG TTGGGGATAG TATTTATGAC 
CATCTTTGTC ATTTAATAGG GCCAGTAGAA TTTGCAAACA CAACCTTTGA ACAAAACCAG 
TGTGTTGACC TTGAAGGTGA AGATGATCTT CTTTCCTCTC TGAGAGAATA TCTTAAAGAA 
AAAGAAGAAC TTTGCGGTAA CTTTGAGATA GATGAATGTG AGGCTTCTGC TCACGAAGGT 
GATACATCAG TAAATAGGAA TGATAGATTA CCACTGGATG GATTAGAAAA TAGTGATCAG 
ACTGGTCAAT GCACGAATAG CATCACTGTT ACAGAAAGAC CTGATAACTC AGAGCTGCAC 
CTAGAACAAA ACCAAAGTGG CTTTATAAGT AATATTGCAC CTACTCAAAT AGACATTTTT 
GCAAGAAATG AAGCACTCGA TAAAGTCAGA CAGTTAAATA TCGATAAACA GTGTGAAAGT 
CTGTTTGATC ATGGGCCAAT TAGTCCTAGC ATCAAAGAAG GAAAAAGATA CCTGTACAGG 
CAAGCAGCCA CTGAAGCTGA TGTAGATTCA GCCATGGAAG CAGCGTTAGC TGAAAAGCAT 
AAAAGTGCTC AATTTAACGA AAAAATAACC AACTCACGTG TTATGCATGC AAAGAAAATT 
CTTCCTAAAG CACAAGTTAA CCCTCAAATG CCTCACATCG CAGGTGCAAG CAAGCTGTCA 
CCAACTAAAA GGAGTATATC TGAAACAGTA ACTCATAGGG CCAAAATCAT GAAAATAACA 
GCTAAAAAAA GAAATAGCGT TCATGTAACT TTTAGACCAT CTACAGAATC AGTTCAGTTT 
TACAATCCTC TTGAGAACAA AGAGGCTGCT TGGAAAGCAA GACTTCGTAA ACTTAGTGGC 
TTCAGCGGTA GTGGTAGCGG CAACAGCAGT AGCAGTACAA GCACCAGCTC ATCAGCTGCC 
ACGGAGCTGG TTAGACCTGG AATATATAGA CCTCTAGATG CAATCAATGC TGCTTCACTT 
GGTAGCAAGC AAAGTAAGGA AATTACAGAA ATTCAAGCAG CTGTGCTGCA AAAGGAAAGT 
GTTGCAGTTA ATCAGTTCCA TAATCGTAGT GCATTTAGAT CATCAGGTCA GGAGTCAGCG 
CAACAGCAGG GCTCCCTGGG TGGTGTTTAT AAAACTGTTG TACATGCTCT TTCGAAGCCG 
AAGGCAAATG TTTCCCCACA GAGGCAGAAC AGAATGCCAG CAGAGGCTCC ACTGAGGGAT 
CTGTACAGTC ATGTAATGGG CTATTTTGGA AGGAAAGCTG CAGCTAATAG AGAGGACATG 
AGTCAAAAGC TGCACCCTAT TGATAGTGAT ATTGGCAGTA GCAATACAAA TGTTCCTGAC 
CTCATGGATG AATTTATAGC TGAGAGACTC CGCAGTGGTA GTACACTGAA TGTGACAAGA 
CGGGGAAGCA GTCCTGGCAG CCTGGAAGTT CCAAAAGATC TTCCTGATGT ATTGAACAAA 
CAGAACCAAA TGCGTCCTAT AGATGACCCA GGCGTGCCTT CCGAATGGAC ATCACCTGCT 
AGCGCAGGCA GCAGTGACCT CATCAGTTCA GATAGCCACT CGGACTCGTT CAGTGCTTTT 
CAGTATGATG GACGCAAATT TGAAAGTACA CTTACAGCGA CTAAGCAGAA TACAGGACAT 
GCAATAGGAA TGTCTTCTCC AGAAGTTCAG TCGCAGTTTT GTGACAGAAA TGAGAATGTC 
CATAATAGCC AATTACTTTC TGCTTTTACA TCTTATGTAA ATTGTGCAAC AGTGTGTTGG 
AAGCATAGGA GATGTACATA CAAGTTTTAC ATGGAGGATT TTGGAGATTT CAGCTTTGGC 
ACAGAGGCAG GGACGCCAGC TTCCACTGAT GCTGATGCAA TTTCAGGACA GCAGCAGAGT 
GCTGAGGAAC AAGAAGTAGC CAGTCTAACT ACACTCCACA TAGATTCGGA AACAAGTAGT 
CTGAATCAGC AACCGTTGTC TGCTGAAGCT GCAACTGTTA CTGGCTCTGA AAGTGCTTCT 
CCAATTCAAT CTGCTCTTGG ATCCCGATCA CAGACACCCT CTCCTGCCAC TCTTCATGCA 
GATCATATTG AGCAGAAGGA TCTGCAACTG GATGAGAAGC TCCACCACTC TGTTTTGCAG 
ACGCCAGATG ACCTAGAAAC TGGTGAATTT CCCTCAGAAT GTTGCAGTGT TATGGCTGGG 
GGAACACTTA CAGGATGGCA CGCAGATGTT GCTACTGTGA TGTGGAGACG AATGCTAGGA 
ATTTTAGGTG ATGTCAACAG CATCATGGAT CCAGAGATTC ATGCCCAGGT TTTTGATTAC 
CTGTGTGAAC TGTGGCAGAA TCTGGCCAAG ATAAGAGACA ATCTTGGTAT TTCAACAGAT 
AACCTCACTT CACCATCTCC ACCAGTTTTA ATCCCACCAC TGAGAATTCT AACACCTTGG 
CTGTTCAAGG CAACAATGCT GACTGATAAA TACAAACAAG GGAAGCTACA TGCTTATAAA 
CTCATTTGTA AGACAATGAA GCGAAGACAA GATGTATCTC CAAACAAGGA TTTTTTGACT 
CATTTCTATA ATATAATGCA CTGTGGACTT CTTCATGTTG ATCAGGATAT TGTCAATACA 
ATCATCAAGC ACTGCTCTCC TCAGTTCTTT TCACTTAGTT TGCCTGGTGC CACCATGCTT 
ATTATGGATT TCATTGTAGC AGCAGGAAGG GTGGCAGCTT CTTCTTTTCT CAATGCACCA 
AGAGTTGAAG CTCAAGTTCT TTTAGGATCT CTTGTCTGTT TTCCCAATTT ATATCATGAA 
CTACCAGCTC TTCACCCAAA CATTCCTGAC ATAGCTGTGT CTCAGTTTAC AGATGTTAAG 
GAGCTCATAA TTAAAACTGT GTTAAGTTCA GCCAGAGAGG AGCCATCTGG TCCTGCAAGA 
TGTGTTGCTC TTTGCAGCCT TGGTATTTGG ATCTGTGAGG AGCTAGTTCA TGAATCTCAT 
CATCCTCAGA TCAAGGAAGC ATTGAATGTG ATTTGTGTTT CTTTAAAGTT TCCGAATAAG 
ACAGTAGCTC AAGTTGCCTG CAGTATGCTG AATATGCTGA TACATTATGT GCATAGACTT 
CAAGTGTATC AAGCTGATTC ACCTTTAAGA ATTATTCAAA TTCTGATAGC TACAATTACC 
CATCTGCTAC CCGGTACAGA AGCTTCATCC TATGAACAGG ACAAGAGGTT GGTAGTTTCC 
TTACTTCTGT GCTTATTGGA TTGGATAATG GCCTTGCCAT TGAAGACCTT GCTGCAGCCT 
CTTCACGCTA CAGGAGCAGA AAATGATAAG ACTGAGAGAT CTGTTCTTAA TTGTATATAT 
AAGGTTCTAC ATGGATGTGT CTATGGATCT CAGTGTTTTA GTAACCCCAA ATATTTTCCC 
CTAAGTCTTT CTGATTTGGC ATGTGTGGAT TACGATCCAT TTATGCATTT AGAAAGCTTG 
AAGGAGCCAG AACCACTGCA TTCTCCAGAC TCAGAGCGTT CTTCTAAACT TCAACCAGTC 
ACCGAAGTGA AAACTCACAT GCAACAAGGA TTAATTTCTG TTGCTGCTCG TACTGTGATA 
ACGCATCTAG TCAACCACTT AGGTCACTAT CCTATGAGTG GAGGACCTGC TATGCTGACT 
AGTCAAGTGT GTGAAAATAA TGACAATCCA TATAGTGAAA GTCCTGAACT TTCTCCTGAA 
CTTTTTGAGA ACCCAAACCT TCAATTCTTT GTGTTAAACA ATACTTCCTT GGTGTCTTGC 
ATACAAATCC AAGCAGAAGA TGACATGCCT GGGGGAGGAC TGTCTGCTGG ACTTGCATCT 
GCTAATTCAA ATGTCAGAAT TATAGTGCGT GATCTGTCTG GCAAATACTC ATGGGATTCA 
GCCATTCTGT ATGGACCATC ATCCTTATGT GCATCTTCAC AACAAACATC TTTTGCCCTT 
TCATTATCTC AACAAGAAAA AACGGAAGAT CCCCTTTCTT CTTTTGAGCA TGTGGAGGAC 
ATATCTGCAA GGGATGGAAT TACACTCCAG GTAAAAAGGA AATTCAGAGA CACTGTGCCA 
ACATGGGATA CAATTAGGGA TGAAGAAGAT GCCCTTGATG AGCTCTTGCA GTATTTAGGT 
GTTACAAGCC CTGAATGCTT ACAGAGAACT GGAGTCTCAC TCAACATCCC TGCTCCTCAA 
CCAGTCTGCA TCTCAGAGAA GCAGGAGAAT GACGTCATCA ATGCAATTCT GAAACAGCAT 
ACAGAAGAGA GAGAGTTTGT TGAAAAACAT TTCAATGATT TAAATATGAG GGCTATGGAG 
CAGGATGAGC CAACATCGCA AAAGCCCCAG TCAGCATTTT ACTACTGCAG ACTACTTCTC 
AGTATACTGG GAATGAATTC TTGGGATAAA AGAAGGAGCT TTCATCTCCT GAAGAAAAAT 
GAAAAGTTAC TTCGAGAGCT CAGAAATTTG GACTCAAGAC AGTGCCGAGA GACCCACAAG 
ATTGCAGTGT TTTATGTTGC TGAGGGACAA GAAGATAAAC ACTCCATTCT TACTAATACT 
GGGGGAAGTC AAGCCTATGA GGATTTTGTA GCTGGCCTTG GCTGGGAGGT AAATCTCACA 
AACCATTGTG GCTTTATGGG AGGACTGCAA AAAAACAAAA GCACTGGATT GACCACTCCA 
TATTTTGCTA CCTCAACAGT AGAAGTAATG TTCCATGTGT CAACAAGAAT GCCTTCTGAT 
TCAGATGACT CTCTAACAAA AAAGCTAAGA CATTTAGGGA ACGATGAAGT ACACATTGTT 
TGGTCAGAGC ATACTCGAGA TTATAGAAGA GGAATAATTC CAACCGAATT TGGTGATGTT 
CTTATTGTTA TCTATCCCAT GAAAAACCAC ATGTTCAGTA TCCAGATCAT GAAGAAGCCT 
GAGGTTCCAT TTTTTGGTCC ACTTTTTGAT GGTGCTATTG TGAATGGAAA AATTCTTCCT 
ATTATGGTTC GCGCAACAGC TATAAATGCA AGCCGTGCAT TGAAATCATT AATCCCTTTA 
TACCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTTCA GCATCACCTA 
GAACCTACAA CTTTTGAGGA TTTTGCTGCT CAGGTTTTCT GTCCAGCACC TTACCACCAT 
TTGCCCTCAG ATGCAGAAAG AAATGCTGTG ATATTTCTTC TCCTTCCAAA ACCTCCACTA 
GAAAGCATTG GCCACATCAA GGTAAAGGGC AAAGGCTTGA AACGGAAGGT CTTTCACGCT 
AGCTGTGAAT GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC 
AGCAGCGCAG GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287373.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142859.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X5, mRNA.

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CloneID	OGa36819
Clone ID Related Accession (Same CDS sequence)	XM_015287373.1 , XM_015287373.2
Accession Version	XM_015287373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGGT GCAGTTCCTG TCATTGACAG TTCATCCCAT 
CACGCTCCAA GTTTACAGAG TTCCACTGAG ACCTCTTCAA TGCATAGATC CACTGAAAGC 
CATATTACTG ATTCAAATAG CAGAGAGTCC TCCTTGGAAG TTGGGGATAG TATTTATGAC 
CATCTTTGTC ATTTAATAGG GCCAGTAGAA TTTGCAAACA CAACCTTTGA ACAAAACCAG 
TGTGTTGACC TTGAAGGTGA AGATGATCTT CTTTCCTCTC TGAGAGAATA TCTTAAAGAA 
AAAGAAGAAC TTTGCGGTAA CTTTGAGATA GATGAATGTG AGGCTTCTGC TCACGAAGGT 
GATACATCAG TAAATAGGAA TGATAGATTA CCACTGGATG GATTAGAAAA TAGTGATCAG 
ACTGGTCAAT GCACGAATAG CATCACTGTT ACAGAAAGAC CTGATAACTC AGAGCTGCAC 
CTAGAACAAA ACCAAAGTGG CTTTATAAGT AATATTGCAC CTACTCAAAT AGACATTTTT 
GCAAGAAATG AAGCACTCGA TAAAGTCAGA CAGTTAAATA TCGATAAACA GTGTGAAAGT 
CTGTTTGATC ATGGGCCAAT TAGTCCTAGC ATCAAAGAAG GAAAAAGATA CCTGTACAGG 
CAAGCAGCCA CTGAAGCTGA TGTAGATTCA GCCATGGAAG CAGCGTTAGC TGAAAAGCAT 
AAAAGTGCTC AATTTAACGA AAAAATAACC AACTCACGTG TTATGCATGC AAAGAAAATT 
CTTCCTAAAG CACAAGTTAA CCCTCAAATG CCTCACATCG CAGGTGCAAG CAAGCTGTCA 
CCAACTAAAA GGAGTATATC TGAAACAGTA ACTCATAGGG CCAAAATCAT GAAAATAACA 
GCTAAAAAAA GAAATAGCGT TCATGTAACT TTTAGACCAT CTACAGAATC AGTTCAGTTT 
TACAATCCTC TTGAGAACAA AGAGGCTGCT TGGAAAGCAA GACTTCGTAA ACTTAGTGGC 
TTCAGCGGTA GTGGTAGCGG CAACAGCAGT AGCAGTACAA GCACCAGCTC ATCAGCTGCC 
ACGGAGCTGG TTAGACCTGG AATATATAGA CCTCTAGATG CAATCAATGC TGCTTCACTT 
GGTAGCAAGC AAAGTAAGGA AATTACAGAA ATTCAAGCAG CTGTGCTGCA AAAGGAAAGT 
GTTGCAGTTA ATCAGTTCCA TAATCGTAGT GCATTTAGAT CATCAGGTCA GGAGTCAGCG 
CAACAGCAGG GCTCCCTGGG TGGTGTTTAT AAAACTGTTG TACATGCTCT TTCGAAGCCG 
AAGGCAAATG TTTCCCCACA GAGGCAGAAC AGAATGCCAG CAGAGGCTCC ACTGAGGGAT 
CTGTACAGTC ATGTAATGGG CTATTTTGGA AGGAAAGCTG CAGCTAATAG AGAGGACATG 
AGTCAAAAGC TGCACCCTAT TGATAGTGAT ATTGGCAGTA GCAATACAAA TGTTCCTGAC 
CTCATGGATG AATTTATAGC TGAGAGACTC CGCAGTGGTA GTACACTGAA TGTGACAAGA 
CGGGGAAGCA GTCCTGGCAG CCTGGAAGTT CCAAAAGATC TTCCTGATGT ATTGAACAAA 
CAGAACCAAA TGCGTCCTAT AGATGACCCA GGCGTGCCTT CCGAATGGAC ATCACCTGCT 
AGCGCAGGCA GCAGTGACCT CATCAGTTCA GATAGCCACT CGGACTCGTT CAGTGCTTTT 
CAGTATGATG GACGCAAATT TGAAAGTACA CTTACAGCGA CTAAGCAGAA TACAGGACAT 
GCAATAGGAA TGTCTTCTCC AGAAGTTCAG TCGCAGTTTT GTGACAGAAA TGAGAATGTC 
CATAATAGCC AATTACTTTC TGCTTTTACA TCTTATGTAA ATTGTGCAAC AGTGTGTTGG 
AAGCATAGGA GATGTACATA CAAGTTTTAC ATGGAGGATT TTGGAGATTT CAGCTTTGGC 
ACAGAGGCAG GGACGCCAGC TTCCACTGAT GCTGATGCAA TTTCAGGACA GCAGCAGAGT 
GCTGAGGAAC AAGAAGTAGC CAGTCTAACT ACACTCCACA TAGATTCGGA AACAAGTAGT 
CTGAATCAGC AACCGTTGTC TGCTGAAGCT GCAACTGTTA CTGGCTCTGA AAGTGCTTCT 
CCAATTCAAT CTGCTCTTGG ATCCCGATCA CAGACACCCT CTCCTGCCAC TCTTCATGCA 
GATCATATTG AGCAGAAGGA TCTGCAACTG GATGAGAAGC TCCACCACTC TGTTTTGCAG 
ACGCCAGATG ACCTAGAAAC TGGTGAATTT CCCTCAGAAT GTTGCAGTGT TATGGCTGGG 
GGAACACTTA CAGGATGGCA CGCAGATGTT GCTACTGTGA TGTGGAGACG AATGCTAGGA 
ATTTTAGGTG ATGTCAACAG CATCATGGAT CCAGAGATTC ATGCCCAGGT TTTTGATTAC 
CTGTGTGAAC TGTGGCAGAA TCTGGCCAAG ATAAGAGACA ATCTTGGTAT TTCAACAGAT 
AACCTCACTT CACCATCTCC ACCAGTTTTA ATCCCACCAC TGAGAATTCT AACACCTTGG 
CTGTTCAAGG CAACAATGCT GACTGATAAA TACAAACAAG GGAAGCTACA TGCTTATAAA 
CTCATTTGTA AGACAATGAA GCGAAGACAA GATGTATCTC CAAACAAGGA TTTTTTGACT 
CATTTCTATA ATATAATGCA CTGTGGACTT CTTCATGTTG ATCAGGATAT TGTCAATACA 
ATCATCAAGC ACTGCTCTCC TCAGTTCTTT TCACTTAGTT TGCCTGGTGC CACCATGCTT 
ATTATGGATT TCATTGTAGC AGCAGGAAGG GTGGCAGCTT CTTCTTTTCT CAATGCACCA 
AGAGTTGAAG CTCAAGTTCT TTTAGGATCT CTTGTCTGTT TTCCCAATTT ATATCATGAA 
CTACCAGCTC TTCACCCAAA CATTCCTGAC ATAGCTGTGT CTCAGTTTAC AGATGTTAAG 
GAGCTCATAA TTAAAACTGT GTTAAGTTCA GCCAGAGAGG AGCCATCTGG TCCTGCAAGA 
TGTGTTGCTC TTTGCAGCCT TGGTATTTGG ATCTGTGAGG AGCTAGTTCA TGAATCTCAT 
CATCCTCAGA TCAAGGAAGC ATTGAATGTG ATTTGTGTTT CTTTAAAGTT TCCGAATAAG 
ACAGTAGCTC AAGTTGCCTG CAGTATGCTG AATATGCTGA TACATTATGT GCATAGACTT 
CAAGTGTATC AAGCTGATTC ACCTTTAAGA ATTATTCAAA TTCTGATAGC TACAATTACC 
CATCTGCTAC CCGGTACAGA AGCTTCATCC TATGAACAGG ACAAGAGGTT GGTAGTTTCC 
TTACTTCTGT GCTTATTGGA TTGGATAATG GCCTTGCCAT TGAAGACCTT GCTGCAGCCT 
CTTCACGCTA CAGGAGCAGA AAATGATAAG ACTGAGAGAT CTGTTCTTAA TTGTATATAT 
AAGGTTCTAC ATGGATGTGT CTATGGATCT CAGTGTTTTA GTAACCCCAA ATATTTTCCC 
CTAAGTCTTT CTGATTTGGC ATGTGTGGAT TACGATCCAT TTATGCATTT AGAAAGCTTG 
AAGGAGCCAG AACCACTGCA TTCTCCAGAC TCAGAGCGTT CTTCTAAACT TCAACCAGTC 
ACCGAAGTGA AAACTCACAT GCAACAAGGA TTAATTTCTG TTGCTGCTCG TACTGTGATA 
ACGCATCTAG TCAACCACTT AGGTCACTAT CCTATGAGTG GAGGACCTGC TATGCTGACT 
AGTCAAGTGT GTGAAAATAA TGACAATCCA TATAGTGAAA GTCCTGAACT TTCTCCTGAA 
CTTTTTGAGA ACCCAAACCT TCAATTCTTT GTGTTAAACA ATACTTCCTT GGTGTCTTGC 
ATACAAATCC AAGCAGAAGA TGACATGCCT GGGGGAGGAC TGTCTGCTGG ACTTGCATCT 
GCTAATTCAA ATGTCAGAAT TATAGTGCGT GATCTGTCTG GCAAATACTC ATGGGATTCA 
GCCATTCTGT ATGGACCATC ATCCTTATGT GCATCTTCAC AACAAACATC TTTTGCCCTT 
TCATTATCTC AACAAGAAAA AACGGAAGAT CCCCTTTCTT CTTTTGAGCA TGTGGAGGAC 
ATATCTGCAA GGGATGGAAT TACACTCCAG GTAAAAAGGA AATTCAGAGA CACTGTGCCA 
ACATGGGATA CAATTAGGGA TGAAGAAGAT GCCCTTGATG AGCTCTTGCA GTATTTAGGT 
GTTACAAGCC CTGAATGCTT ACAGAGAACT GGAGTCTCAC TCAACATCCC TGCTCCTCAA 
CCAGTCTGCA TCTCAGAGAA GCAGGAGAAT GACGTCATCA ATGCAATTCT GAAACAGCAT 
ACAGAAGAGA GAGAGTTTGT TGAAAAACAT TTCAATGATT TAAATATGAG GGCTATGGAG 
CAGGATGAGC CAACATCGCA AAAGCCCCAG TCAGCATTTT ACTACTGCAG ACTACTTCTC 
AGTATACTGG GAATGAATTC TTGGGATAAA AGAAGGAGCT TTCATCTCCT GAAGAAAAAT 
GAAAAGTTAC TTCGAGAGCT CAGAAATTTG GACTCAAGAC AGTGCCGAGA GACCCACAAG 
ATTGCAGTGT TTTATGTTGC TGAGGGACAA GAAGATAAAC ACTCCATTCT TACTAATACT 
GGGGGAAGTC AAGCCTATGA GGATTTTGTA GCTGGCCTTG GCTGGGAGGT AAATCTCACA 
AACCATTGTG GCTTTATGGG AGGACTGCAA AAAAACAAAA GCACTGGATT GACCACTCCA 
TATTTTGCTA CCTCAACAGT AGAAGTAATG TTCCATGTGT CAACAAGAAT GCCTTCTGAT 
TCAGATGACT CTCTAACAAA AAAGCTAAGA CATTTAGGGA ACGATGAAGT ACACATTGTT 
TGGTCAGAGC ATACTCGAGA TTATAGAAGA GGAATAATTC CAACCGAATT TGGTGATGTT 
CTTATTGTTA TCTATCCCAT GAAAAACCAC ATGTTCAGTA TCCAGATCAT GAAGAAGCCT 
GAGGTTCCAT TTTTTGGTCC ACTTTTTGAT GGTGCTATTG TGAATGGAAA AATTCTTCCT 
ATTATGGTTC GCGCAACAGC TATAAATGCA AGCCGTGCAT TGAAATCATT AATCCCTTTA 
TACCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTTCA GCATCACCTA 
GAACCTACAA CTTTTGAGGA TTTTGCTGCT CAGGTTTTCT GTCCAGCACC TTACCACCAT 
TTGCCCTCAG ATGCAGAAAG AAATGCTGTG ATATTTCTTC TCCTTCCAAA ACCTCCACTA 
GAAAGCATTG GCCACATCAA GGTAAAGGGC AAAGGCTTGA AACGGAAGGT CTTTCACGCT 
AGCTGTGAAT GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC 
AGCAGCGCAG GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142859.1
CDS	268..8181
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287373.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287373.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGGT GCAGTTCCTG TCATTGACAG TTCATCCCAT 
CACGCTCCAA GTTTACAGAG TTCCACTGAG ACCTCTTCAA TGCATAGATC CACTGAAAGC 
CATATTACTG ATTCAAATAG CAGAGAGTCC TCCTTGGAAG TTGGGGATAG TATTTATGAC 
CATCTTTGTC ATTTAATAGG GCCAGTAGAA TTTGCAAACA CAACCTTTGA ACAAAACCAG 
TGTGTTGACC TTGAAGGTGA AGATGATCTT CTTTCCTCTC TGAGAGAATA TCTTAAAGAA 
AAAGAAGAAC TTTGCGGTAA CTTTGAGATA GATGAATGTG AGGCTTCTGC TCACGAAGGT 
GATACATCAG TAAATAGGAA TGATAGATTA CCACTGGATG GATTAGAAAA TAGTGATCAG 
ACTGGTCAAT GCACGAATAG CATCACTGTT ACAGAAAGAC CTGATAACTC AGAGCTGCAC 
CTAGAACAAA ACCAAAGTGG CTTTATAAGT AATATTGCAC CTACTCAAAT AGACATTTTT 
GCAAGAAATG AAGCACTCGA TAAAGTCAGA CAGTTAAATA TCGATAAACA GTGTGAAAGT 
CTGTTTGATC ATGGGCCAAT TAGTCCTAGC ATCAAAGAAG GAAAAAGATA CCTGTACAGG 
CAAGCAGCCA CTGAAGCTGA TGTAGATTCA GCCATGGAAG CAGCGTTAGC TGAAAAGCAT 
AAAAGTGCTC AATTTAACGA AAAAATAACC AACTCACGTG TTATGCATGC AAAGAAAATT 
CTTCCTAAAG CACAAGTTAA CCCTCAAATG CCTCACATCG CAGGTGCAAG CAAGCTGTCA 
CCAACTAAAA GGAGTATATC TGAAACAGTA ACTCATAGGG CCAAAATCAT GAAAATAACA 
GCTAAAAAAA GAAATAGCGT TCATGTAACT TTTAGACCAT CTACAGAATC AGTTCAGTTT 
TACAATCCTC TTGAGAACAA AGAGGCTGCT TGGAAAGCAA GACTTCGTAA ACTTAGTGGC 
TTCAGCGGTA GTGGTAGCGG CAACAGCAGT AGCAGTACAA GCACCAGCTC ATCAGCTGCC 
ACGGAGCTGG TTAGACCTGG AATATATAGA CCTCTAGATG CAATCAATGC TGCTTCACTT 
GGTAGCAAGC AAAGTAAGGA AATTACAGAA ATTCAAGCAG CTGTGCTGCA AAAGGAAAGT 
GTTGCAGTTA ATCAGTTCCA TAATCGTAGT GCATTTAGAT CATCAGGTCA GGAGTCAGCG 
CAACAGCAGG GCTCCCTGGG TGGTGTTTAT AAAACTGTTG TACATGCTCT TTCGAAGCCG 
AAGGCAAATG TTTCCCCACA GAGGCAGAAC AGAATGCCAG CAGAGGCTCC ACTGAGGGAT 
CTGTACAGTC ATGTAATGGG CTATTTTGGA AGGAAAGCTG CAGCTAATAG AGAGGACATG 
AGTCAAAAGC TGCACCCTAT TGATAGTGAT ATTGGCAGTA GCAATACAAA TGTTCCTGAC 
CTCATGGATG AATTTATAGC TGAGAGACTC CGCAGTGGTA GTACACTGAA TGTGACAAGA 
CGGGGAAGCA GTCCTGGCAG CCTGGAAGTT CCAAAAGATC TTCCTGATGT ATTGAACAAA 
CAGAACCAAA TGCGTCCTAT AGATGACCCA GGCGTGCCTT CCGAATGGAC ATCACCTGCT 
AGCGCAGGCA GCAGTGACCT CATCAGTTCA GATAGCCACT CGGACTCGTT CAGTGCTTTT 
CAGTATGATG GACGCAAATT TGAAAGTACA CTTACAGCGA CTAAGCAGAA TACAGGACAT 
GCAATAGGAA TGTCTTCTCC AGAAGTTCAG TCGCAGTTTT GTGACAGAAA TGAGAATGTC 
CATAATAGCC AATTACTTTC TGCTTTTACA TCTTATGTAA ATTGTGCAAC AGTGTGTTGG 
AAGCATAGGA GATGTACATA CAAGTTTTAC ATGGAGGATT TTGGAGATTT CAGCTTTGGC 
ACAGAGGCAG GGACGCCAGC TTCCACTGAT GCTGATGCAA TTTCAGGACA GCAGCAGAGT 
GCTGAGGAAC AAGAAGTAGC CAGTCTAACT ACACTCCACA TAGATTCGGA AACAAGTAGT 
CTGAATCAGC AACCGTTGTC TGCTGAAGCT GCAACTGTTA CTGGCTCTGA AAGTGCTTCT 
CCAATTCAAT CTGCTCTTGG ATCCCGATCA CAGACACCCT CTCCTGCCAC TCTTCATGCA 
GATCATATTG AGCAGAAGGA TCTGCAACTG GATGAGAAGC TCCACCACTC TGTTTTGCAG 
ACGCCAGATG ACCTAGAAAC TGGTGAATTT CCCTCAGAAT GTTGCAGTGT TATGGCTGGG 
GGAACACTTA CAGGATGGCA CGCAGATGTT GCTACTGTGA TGTGGAGACG AATGCTAGGA 
ATTTTAGGTG ATGTCAACAG CATCATGGAT CCAGAGATTC ATGCCCAGGT TTTTGATTAC 
CTGTGTGAAC TGTGGCAGAA TCTGGCCAAG ATAAGAGACA ATCTTGGTAT TTCAACAGAT 
AACCTCACTT CACCATCTCC ACCAGTTTTA ATCCCACCAC TGAGAATTCT AACACCTTGG 
CTGTTCAAGG CAACAATGCT GACTGATAAA TACAAACAAG GGAAGCTACA TGCTTATAAA 
CTCATTTGTA AGACAATGAA GCGAAGACAA GATGTATCTC CAAACAAGGA TTTTTTGACT 
CATTTCTATA ATATAATGCA CTGTGGACTT CTTCATGTTG ATCAGGATAT TGTCAATACA 
ATCATCAAGC ACTGCTCTCC TCAGTTCTTT TCACTTAGTT TGCCTGGTGC CACCATGCTT 
ATTATGGATT TCATTGTAGC AGCAGGAAGG GTGGCAGCTT CTTCTTTTCT CAATGCACCA 
AGAGTTGAAG CTCAAGTTCT TTTAGGATCT CTTGTCTGTT TTCCCAATTT ATATCATGAA 
CTACCAGCTC TTCACCCAAA CATTCCTGAC ATAGCTGTGT CTCAGTTTAC AGATGTTAAG 
GAGCTCATAA TTAAAACTGT GTTAAGTTCA GCCAGAGAGG AGCCATCTGG TCCTGCAAGA 
TGTGTTGCTC TTTGCAGCCT TGGTATTTGG ATCTGTGAGG AGCTAGTTCA TGAATCTCAT 
CATCCTCAGA TCAAGGAAGC ATTGAATGTG ATTTGTGTTT CTTTAAAGTT TCCGAATAAG 
ACAGTAGCTC AAGTTGCCTG CAGTATGCTG AATATGCTGA TACATTATGT GCATAGACTT 
CAAGTGTATC AAGCTGATTC ACCTTTAAGA ATTATTCAAA TTCTGATAGC TACAATTACC 
CATCTGCTAC CCGGTACAGA AGCTTCATCC TATGAACAGG ACAAGAGGTT GGTAGTTTCC 
TTACTTCTGT GCTTATTGGA TTGGATAATG GCCTTGCCAT TGAAGACCTT GCTGCAGCCT 
CTTCACGCTA CAGGAGCAGA AAATGATAAG ACTGAGAGAT CTGTTCTTAA TTGTATATAT 
AAGGTTCTAC ATGGATGTGT CTATGGATCT CAGTGTTTTA GTAACCCCAA ATATTTTCCC 
CTAAGTCTTT CTGATTTGGC ATGTGTGGAT TACGATCCAT TTATGCATTT AGAAAGCTTG 
AAGGAGCCAG AACCACTGCA TTCTCCAGAC TCAGAGCGTT CTTCTAAACT TCAACCAGTC 
ACCGAAGTGA AAACTCACAT GCAACAAGGA TTAATTTCTG TTGCTGCTCG TACTGTGATA 
ACGCATCTAG TCAACCACTT AGGTCACTAT CCTATGAGTG GAGGACCTGC TATGCTGACT 
AGTCAAGTGT GTGAAAATAA TGACAATCCA TATAGTGAAA GTCCTGAACT TTCTCCTGAA 
CTTTTTGAGA ACCCAAACCT TCAATTCTTT GTGTTAAACA ATACTTCCTT GGTGTCTTGC 
ATACAAATCC AAGCAGAAGA TGACATGCCT GGGGGAGGAC TGTCTGCTGG ACTTGCATCT 
GCTAATTCAA ATGTCAGAAT TATAGTGCGT GATCTGTCTG GCAAATACTC ATGGGATTCA 
GCCATTCTGT ATGGACCATC ATCCTTATGT GCATCTTCAC AACAAACATC TTTTGCCCTT 
TCATTATCTC AACAAGAAAA AACGGAAGAT CCCCTTTCTT CTTTTGAGCA TGTGGAGGAC 
ATATCTGCAA GGGATGGAAT TACACTCCAG GTAAAAAGGA AATTCAGAGA CACTGTGCCA 
ACATGGGATA CAATTAGGGA TGAAGAAGAT GCCCTTGATG AGCTCTTGCA GTATTTAGGT 
GTTACAAGCC CTGAATGCTT ACAGAGAACT GGAGTCTCAC TCAACATCCC TGCTCCTCAA 
CCAGTCTGCA TCTCAGAGAA GCAGGAGAAT GACGTCATCA ATGCAATTCT GAAACAGCAT 
ACAGAAGAGA GAGAGTTTGT TGAAAAACAT TTCAATGATT TAAATATGAG GGCTATGGAG 
CAGGATGAGC CAACATCGCA AAAGCCCCAG TCAGCATTTT ACTACTGCAG ACTACTTCTC 
AGTATACTGG GAATGAATTC TTGGGATAAA AGAAGGAGCT TTCATCTCCT GAAGAAAAAT 
GAAAAGTTAC TTCGAGAGCT CAGAAATTTG GACTCAAGAC AGTGCCGAGA GACCCACAAG 
ATTGCAGTGT TTTATGTTGC TGAGGGACAA GAAGATAAAC ACTCCATTCT TACTAATACT 
GGGGGAAGTC AAGCCTATGA GGATTTTGTA GCTGGCCTTG GCTGGGAGGT AAATCTCACA 
AACCATTGTG GCTTTATGGG AGGACTGCAA AAAAACAAAA GCACTGGATT GACCACTCCA 
TATTTTGCTA CCTCAACAGT AGAAGTAATG TTCCATGTGT CAACAAGAAT GCCTTCTGAT 
TCAGATGACT CTCTAACAAA AAAGCTAAGA CATTTAGGGA ACGATGAAGT ACACATTGTT 
TGGTCAGAGC ATACTCGAGA TTATAGAAGA GGAATAATTC CAACCGAATT TGGTGATGTT 
CTTATTGTTA TCTATCCCAT GAAAAACCAC ATGTTCAGTA TCCAGATCAT GAAGAAGCCT 
GAGGTTCCAT TTTTTGGTCC ACTTTTTGAT GGTGCTATTG TGAATGGAAA AATTCTTCCT 
ATTATGGTTC GCGCAACAGC TATAAATGCA AGCCGTGCAT TGAAATCATT AATCCCTTTA 
TACCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTTCA GCATCACCTA 
GAACCTACAA CTTTTGAGGA TTTTGCTGCT CAGGTTTTCT GTCCAGCACC TTACCACCAT 
TTGCCCTCAG ATGCAGAAAG AAATGCTGTG ATATTTCTTC TCCTTCCAAA ACCTCCACTA 
GAAAGCATTG GCCACATCAA GGTAAAGGGC AAAGGCTTGA AACGGAAGGT CTTTCACGCT 
AGCTGTGAAT GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC 
AGCAGCGCAG GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287374.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142860.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.

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CloneID	OGa36820
Clone ID Related Accession (Same CDS sequence)	XM_015287374.2 , XM_015287374.1
Accession Version	XM_015287374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGAAACTG GTGAATTTCC CTCAGAATGT TGCAGTGTTA TGGCTGGGGG AACACTTACA 
GGATGGCACG CAGATGTTGC TACTGTGATG TGGAGACGAA TGCTAGGAAT TTTAGGTGAT 
GTCAACAGCA TCATGGATCC AGAGATTCAT GCCCAGGTTT TTGATTACCT GTGTGAACTG 
TGGCAGAATC TGGCCAAGAT AAGAGACAAT CTTGGTATTT CAACAGATAA CCTCACTTCA 
CCATCTCCAC CAGTTTTAAT CCCACCACTG AGAATTCTAA CACCTTGGCT GTTCAAGGCA 
ACAATGCTGA CTGATAAATA CAAACAAGGG AAGCTACATG CTTATAAACT CATTTGTAAG 
ACAATGAAGC GAAGACAAGA TGTATCTCCA AACAAGGATT TTTTGACTCA TTTCTATAAT 
ATAATGCACT GTGGACTTCT TCATGTTGAT CAGGATATTG TCAATACAAT CATCAAGCAC 
TGCTCTCCTC AGTTCTTTTC ACTTAGTTTG CCTGGTGCCA CCATGCTTAT TATGGATTTC 
ATTGTAGCAG CAGGAAGGGT GGCAGCTTCT TCTTTTCTCA ATGCACCAAG AGTTGAAGCT 
CAAGTTCTTT TAGGATCTCT TGTCTGTTTT CCCAATTTAT ATCATGAACT ACCAGCTCTT 
CACCCAAACA TTCCTGACAT AGCTGTGTCT CAGTTTACAG ATGTTAAGGA GCTCATAATT 
AAAACTGTGT TAAGTTCAGC CAGAGAGGAG CCATCTGGTC CTGCAAGATG TGTTGCTCTT 
TGCAGCCTTG GTATTTGGAT CTGTGAGGAG CTAGTTCATG AATCTCATCA TCCTCAGATC 
AAGGAAGCAT TGAATGTGAT TTGTGTTTCT TTAAAGTTTC CGAATAAGAC AGTAGCTCAA 
GTTGCCTGCA GTATGCTGAA TATGCTGATA CATTATGTGC ATAGACTTCA AGTGTATCAA 
GCTGATTCAC CTTTAAGAAT TATTCAAATT CTGATAGCTA CAATTACCCA TCTGCTACCC 
GGTACAGAAG CTTCATCCTA TGAACAGGAC AAGAGGTTGG TAGTTTCCTT ACTTCTGTGC 
TTATTGGATT GGATAATGGC CTTGCCATTG AAGACCTTGC TGCAGCCTCT TCACGCTACA 
GGAGCAGAAA ATGATAAGAC TGAGAGATCT GTTCTTAATT GTATATATAA GGTTCTACAT 
GGATGTGTCT ATGGATCTCA GTGTTTTAGT AACCCCAAAT ATTTTCCCCT AAGTCTTTCT 
GATTTGGCAT GTGTGGATTA CGATCCATTT ATGCATTTAG AAAGCTTGAA GGAGCCAGAA 
CCACTGCATT CTCCAGACTC AGAGCGTTCT TCTAAACTTC AACCAGTCAC CGAAGTGAAA 
ACTCACATGC AACAAGGATT AATTTCTGTT GCTGCTCGTA CTGTGATAAC GCATCTAGTC 
AACCACTTAG GTCACTATCC TATGAGTGGA GGACCTGCTA TGCTGACTAG TCAAGTGTGT 
GAAAATAATG ACAATCCATA TAGTGAAAGT CCTGAACTTT CTCCTGAACT TTTTGAGAAC 
CCAAACCTTC AATTCTTTGT GTTAAACAAT ACTTCCTTGG TGTCTTGCAT ACAAATCCAA 
GCAGAAGATG ACATGCCTGG GGGAGGACTG TCTGCTGGAC TTGCATCTGC TAATTCAAAT 
GTCAGAATTA TAGTGCGTGA TCTGTCTGGC AAATACTCAT GGGATTCAGC CATTCTGTAT 
GGACCATCAT CCTTATGTGC ATCTTCACAA CAAACATCTT TTGCCCTTTC ATTATCTCAA 
CAAGAAAAAA CGGAAGATCC CCTTTCTTCT TTTGAGCATG TGGAGGACAT ATCTGCAAGG 
GATGGAATTA CACTCCAGGT AAAAAGGAAA TTCAGAGACA CTGTGCCAAC ATGGGATACA 
ATTAGGGATG AAGAAGATGC CCTTGATGAG CTCTTGCAGT ATTTAGGTGT TACAAGCCCT 
GAATGCTTAC AGAGAACTGG AGTCTCACTC AACATCCCTG CTCCTCAACC AGTCTGCATC 
TCAGAGAAGC AGGAGAATGA CGTCATCAAT GCAATTCTGA AACAGCATAC AGAAGAGAGA 
GAGTTTGTTG AAAAACATTT CAATGATTTA AATATGAGGG CTATGGAGCA GGATGAGCCA 
ACATCGCAAA AGCCCCAGTC AGCATTTTAC TACTGCAGAC TACTTCTCAG TATACTGGGA 
ATGAATTCTT GGGATAAAAG AAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGTTACTT 
CGAGAGCTCA GAAATTTGGA CTCAAGACAG TGCCGAGAGA CCCACAAGAT TGCAGTGTTT 
TATGTTGCTG AGGGACAAGA AGATAAACAC TCCATTCTTA CTAATACTGG GGGAAGTCAA 
GCCTATGAGG ATTTTGTAGC TGGCCTTGGC TGGGAGGTAA ATCTCACAAA CCATTGTGGC 
TTTATGGGAG GACTGCAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC 
TCAACAGTAG AAGTAATGTT CCATGTGTCA ACAAGAATGC CTTCTGATTC AGATGACTCT 
CTAACAAAAA AGCTAAGACA TTTAGGGAAC GATGAAGTAC ACATTGTTTG GTCAGAGCAT 
ACTCGAGATT ATAGAAGAGG AATAATTCCA ACCGAATTTG GTGATGTTCT TATTGTTATC 
TATCCCATGA AAAACCACAT GTTCAGTATC CAGATCATGA AGAAGCCTGA GGTTCCATTT 
TTTGGTCCAC TTTTTGATGG TGCTATTGTG AATGGAAAAA TTCTTCCTAT TATGGTTCGC 
GCAACAGCTA TAAATGCAAG CCGTGCATTG AAATCATTAA TCCCTTTATA CCAAAACTTC 
TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTTCAGC ATCACCTAGA ACCTACAACT 
TTTGAGGATT TTGCTGCTCA GGTTTTCTGT CCAGCACCTT ACCACCATTT GCCCTCAGAT 
GCAGAAAGAA ATGCTGTGAT ATTTCTTCTC CTTCCAAAAC CTCCACTAGA AAGCATTGGC 
CACATCAAGG TAAAGGGCAA AGGCTTGAAA CGGAAGGTCT TTCACGCTAG CTGTGAATGG 
CTCCTGCCCA GAGACTCAGC CGGGAGAGCC TCCAGCAGCA GCAGCAGCAG CAGCGCAGGC 
GGATGGAACC GACTTAGCCT CACCCATGTC TCCTCGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142860.1
CDS	269..8170
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287374.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287374.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGAAACTG GTGAATTTCC CTCAGAATGT TGCAGTGTTA TGGCTGGGGG AACACTTACA 
GGATGGCACG CAGATGTTGC TACTGTGATG TGGAGACGAA TGCTAGGAAT TTTAGGTGAT 
GTCAACAGCA TCATGGATCC AGAGATTCAT GCCCAGGTTT TTGATTACCT GTGTGAACTG 
TGGCAGAATC TGGCCAAGAT AAGAGACAAT CTTGGTATTT CAACAGATAA CCTCACTTCA 
CCATCTCCAC CAGTTTTAAT CCCACCACTG AGAATTCTAA CACCTTGGCT GTTCAAGGCA 
ACAATGCTGA CTGATAAATA CAAACAAGGG AAGCTACATG CTTATAAACT CATTTGTAAG 
ACAATGAAGC GAAGACAAGA TGTATCTCCA AACAAGGATT TTTTGACTCA TTTCTATAAT 
ATAATGCACT GTGGACTTCT TCATGTTGAT CAGGATATTG TCAATACAAT CATCAAGCAC 
TGCTCTCCTC AGTTCTTTTC ACTTAGTTTG CCTGGTGCCA CCATGCTTAT TATGGATTTC 
ATTGTAGCAG CAGGAAGGGT GGCAGCTTCT TCTTTTCTCA ATGCACCAAG AGTTGAAGCT 
CAAGTTCTTT TAGGATCTCT TGTCTGTTTT CCCAATTTAT ATCATGAACT ACCAGCTCTT 
CACCCAAACA TTCCTGACAT AGCTGTGTCT CAGTTTACAG ATGTTAAGGA GCTCATAATT 
AAAACTGTGT TAAGTTCAGC CAGAGAGGAG CCATCTGGTC CTGCAAGATG TGTTGCTCTT 
TGCAGCCTTG GTATTTGGAT CTGTGAGGAG CTAGTTCATG AATCTCATCA TCCTCAGATC 
AAGGAAGCAT TGAATGTGAT TTGTGTTTCT TTAAAGTTTC CGAATAAGAC AGTAGCTCAA 
GTTGCCTGCA GTATGCTGAA TATGCTGATA CATTATGTGC ATAGACTTCA AGTGTATCAA 
GCTGATTCAC CTTTAAGAAT TATTCAAATT CTGATAGCTA CAATTACCCA TCTGCTACCC 
GGTACAGAAG CTTCATCCTA TGAACAGGAC AAGAGGTTGG TAGTTTCCTT ACTTCTGTGC 
TTATTGGATT GGATAATGGC CTTGCCATTG AAGACCTTGC TGCAGCCTCT TCACGCTACA 
GGAGCAGAAA ATGATAAGAC TGAGAGATCT GTTCTTAATT GTATATATAA GGTTCTACAT 
GGATGTGTCT ATGGATCTCA GTGTTTTAGT AACCCCAAAT ATTTTCCCCT AAGTCTTTCT 
GATTTGGCAT GTGTGGATTA CGATCCATTT ATGCATTTAG AAAGCTTGAA GGAGCCAGAA 
CCACTGCATT CTCCAGACTC AGAGCGTTCT TCTAAACTTC AACCAGTCAC CGAAGTGAAA 
ACTCACATGC AACAAGGATT AATTTCTGTT GCTGCTCGTA CTGTGATAAC GCATCTAGTC 
AACCACTTAG GTCACTATCC TATGAGTGGA GGACCTGCTA TGCTGACTAG TCAAGTGTGT 
GAAAATAATG ACAATCCATA TAGTGAAAGT CCTGAACTTT CTCCTGAACT TTTTGAGAAC 
CCAAACCTTC AATTCTTTGT GTTAAACAAT ACTTCCTTGG TGTCTTGCAT ACAAATCCAA 
GCAGAAGATG ACATGCCTGG GGGAGGACTG TCTGCTGGAC TTGCATCTGC TAATTCAAAT 
GTCAGAATTA TAGTGCGTGA TCTGTCTGGC AAATACTCAT GGGATTCAGC CATTCTGTAT 
GGACCATCAT CCTTATGTGC ATCTTCACAA CAAACATCTT TTGCCCTTTC ATTATCTCAA 
CAAGAAAAAA CGGAAGATCC CCTTTCTTCT TTTGAGCATG TGGAGGACAT ATCTGCAAGG 
GATGGAATTA CACTCCAGGT AAAAAGGAAA TTCAGAGACA CTGTGCCAAC ATGGGATACA 
ATTAGGGATG AAGAAGATGC CCTTGATGAG CTCTTGCAGT ATTTAGGTGT TACAAGCCCT 
GAATGCTTAC AGAGAACTGG AGTCTCACTC AACATCCCTG CTCCTCAACC AGTCTGCATC 
TCAGAGAAGC AGGAGAATGA CGTCATCAAT GCAATTCTGA AACAGCATAC AGAAGAGAGA 
GAGTTTGTTG AAAAACATTT CAATGATTTA AATATGAGGG CTATGGAGCA GGATGAGCCA 
ACATCGCAAA AGCCCCAGTC AGCATTTTAC TACTGCAGAC TACTTCTCAG TATACTGGGA 
ATGAATTCTT GGGATAAAAG AAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGTTACTT 
CGAGAGCTCA GAAATTTGGA CTCAAGACAG TGCCGAGAGA CCCACAAGAT TGCAGTGTTT 
TATGTTGCTG AGGGACAAGA AGATAAACAC TCCATTCTTA CTAATACTGG GGGAAGTCAA 
GCCTATGAGG ATTTTGTAGC TGGCCTTGGC TGGGAGGTAA ATCTCACAAA CCATTGTGGC 
TTTATGGGAG GACTGCAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC 
TCAACAGTAG AAGTAATGTT CCATGTGTCA ACAAGAATGC CTTCTGATTC AGATGACTCT 
CTAACAAAAA AGCTAAGACA TTTAGGGAAC GATGAAGTAC ACATTGTTTG GTCAGAGCAT 
ACTCGAGATT ATAGAAGAGG AATAATTCCA ACCGAATTTG GTGATGTTCT TATTGTTATC 
TATCCCATGA AAAACCACAT GTTCAGTATC CAGATCATGA AGAAGCCTGA GGTTCCATTT 
TTTGGTCCAC TTTTTGATGG TGCTATTGTG AATGGAAAAA TTCTTCCTAT TATGGTTCGC 
GCAACAGCTA TAAATGCAAG CCGTGCATTG AAATCATTAA TCCCTTTATA CCAAAACTTC 
TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTTCAGC ATCACCTAGA ACCTACAACT 
TTTGAGGATT TTGCTGCTCA GGTTTTCTGT CCAGCACCTT ACCACCATTT GCCCTCAGAT 
GCAGAAAGAA ATGCTGTGAT ATTTCTTCTC CTTCCAAAAC CTCCACTAGA AAGCATTGGC 
CACATCAAGG TAAAGGGCAA AGGCTTGAAA CGGAAGGTCT TTCACGCTAG CTGTGAATGG 
CTCCTGCCCA GAGACTCAGC CGGGAGAGCC TCCAGCAGCA GCAGCAGCAG CAGCGCAGGC 
GGATGGAACC GACTTAGCCT CACCCATGTC TCCTCGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287374.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142860.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.

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CloneID	OGa36820
Clone ID Related Accession (Same CDS sequence)	XM_015287374.2 , XM_015287374.1
Accession Version	XM_015287374.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGAAACTG GTGAATTTCC CTCAGAATGT TGCAGTGTTA TGGCTGGGGG AACACTTACA 
GGATGGCACG CAGATGTTGC TACTGTGATG TGGAGACGAA TGCTAGGAAT TTTAGGTGAT 
GTCAACAGCA TCATGGATCC AGAGATTCAT GCCCAGGTTT TTGATTACCT GTGTGAACTG 
TGGCAGAATC TGGCCAAGAT AAGAGACAAT CTTGGTATTT CAACAGATAA CCTCACTTCA 
CCATCTCCAC CAGTTTTAAT CCCACCACTG AGAATTCTAA CACCTTGGCT GTTCAAGGCA 
ACAATGCTGA CTGATAAATA CAAACAAGGG AAGCTACATG CTTATAAACT CATTTGTAAG 
ACAATGAAGC GAAGACAAGA TGTATCTCCA AACAAGGATT TTTTGACTCA TTTCTATAAT 
ATAATGCACT GTGGACTTCT TCATGTTGAT CAGGATATTG TCAATACAAT CATCAAGCAC 
TGCTCTCCTC AGTTCTTTTC ACTTAGTTTG CCTGGTGCCA CCATGCTTAT TATGGATTTC 
ATTGTAGCAG CAGGAAGGGT GGCAGCTTCT TCTTTTCTCA ATGCACCAAG AGTTGAAGCT 
CAAGTTCTTT TAGGATCTCT TGTCTGTTTT CCCAATTTAT ATCATGAACT ACCAGCTCTT 
CACCCAAACA TTCCTGACAT AGCTGTGTCT CAGTTTACAG ATGTTAAGGA GCTCATAATT 
AAAACTGTGT TAAGTTCAGC CAGAGAGGAG CCATCTGGTC CTGCAAGATG TGTTGCTCTT 
TGCAGCCTTG GTATTTGGAT CTGTGAGGAG CTAGTTCATG AATCTCATCA TCCTCAGATC 
AAGGAAGCAT TGAATGTGAT TTGTGTTTCT TTAAAGTTTC CGAATAAGAC AGTAGCTCAA 
GTTGCCTGCA GTATGCTGAA TATGCTGATA CATTATGTGC ATAGACTTCA AGTGTATCAA 
GCTGATTCAC CTTTAAGAAT TATTCAAATT CTGATAGCTA CAATTACCCA TCTGCTACCC 
GGTACAGAAG CTTCATCCTA TGAACAGGAC AAGAGGTTGG TAGTTTCCTT ACTTCTGTGC 
TTATTGGATT GGATAATGGC CTTGCCATTG AAGACCTTGC TGCAGCCTCT TCACGCTACA 
GGAGCAGAAA ATGATAAGAC TGAGAGATCT GTTCTTAATT GTATATATAA GGTTCTACAT 
GGATGTGTCT ATGGATCTCA GTGTTTTAGT AACCCCAAAT ATTTTCCCCT AAGTCTTTCT 
GATTTGGCAT GTGTGGATTA CGATCCATTT ATGCATTTAG AAAGCTTGAA GGAGCCAGAA 
CCACTGCATT CTCCAGACTC AGAGCGTTCT TCTAAACTTC AACCAGTCAC CGAAGTGAAA 
ACTCACATGC AACAAGGATT AATTTCTGTT GCTGCTCGTA CTGTGATAAC GCATCTAGTC 
AACCACTTAG GTCACTATCC TATGAGTGGA GGACCTGCTA TGCTGACTAG TCAAGTGTGT 
GAAAATAATG ACAATCCATA TAGTGAAAGT CCTGAACTTT CTCCTGAACT TTTTGAGAAC 
CCAAACCTTC AATTCTTTGT GTTAAACAAT ACTTCCTTGG TGTCTTGCAT ACAAATCCAA 
GCAGAAGATG ACATGCCTGG GGGAGGACTG TCTGCTGGAC TTGCATCTGC TAATTCAAAT 
GTCAGAATTA TAGTGCGTGA TCTGTCTGGC AAATACTCAT GGGATTCAGC CATTCTGTAT 
GGACCATCAT CCTTATGTGC ATCTTCACAA CAAACATCTT TTGCCCTTTC ATTATCTCAA 
CAAGAAAAAA CGGAAGATCC CCTTTCTTCT TTTGAGCATG TGGAGGACAT ATCTGCAAGG 
GATGGAATTA CACTCCAGGT AAAAAGGAAA TTCAGAGACA CTGTGCCAAC ATGGGATACA 
ATTAGGGATG AAGAAGATGC CCTTGATGAG CTCTTGCAGT ATTTAGGTGT TACAAGCCCT 
GAATGCTTAC AGAGAACTGG AGTCTCACTC AACATCCCTG CTCCTCAACC AGTCTGCATC 
TCAGAGAAGC AGGAGAATGA CGTCATCAAT GCAATTCTGA AACAGCATAC AGAAGAGAGA 
GAGTTTGTTG AAAAACATTT CAATGATTTA AATATGAGGG CTATGGAGCA GGATGAGCCA 
ACATCGCAAA AGCCCCAGTC AGCATTTTAC TACTGCAGAC TACTTCTCAG TATACTGGGA 
ATGAATTCTT GGGATAAAAG AAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGTTACTT 
CGAGAGCTCA GAAATTTGGA CTCAAGACAG TGCCGAGAGA CCCACAAGAT TGCAGTGTTT 
TATGTTGCTG AGGGACAAGA AGATAAACAC TCCATTCTTA CTAATACTGG GGGAAGTCAA 
GCCTATGAGG ATTTTGTAGC TGGCCTTGGC TGGGAGGTAA ATCTCACAAA CCATTGTGGC 
TTTATGGGAG GACTGCAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC 
TCAACAGTAG AAGTAATGTT CCATGTGTCA ACAAGAATGC CTTCTGATTC AGATGACTCT 
CTAACAAAAA AGCTAAGACA TTTAGGGAAC GATGAAGTAC ACATTGTTTG GTCAGAGCAT 
ACTCGAGATT ATAGAAGAGG AATAATTCCA ACCGAATTTG GTGATGTTCT TATTGTTATC 
TATCCCATGA AAAACCACAT GTTCAGTATC CAGATCATGA AGAAGCCTGA GGTTCCATTT 
TTTGGTCCAC TTTTTGATGG TGCTATTGTG AATGGAAAAA TTCTTCCTAT TATGGTTCGC 
GCAACAGCTA TAAATGCAAG CCGTGCATTG AAATCATTAA TCCCTTTATA CCAAAACTTC 
TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTTCAGC ATCACCTAGA ACCTACAACT 
TTTGAGGATT TTGCTGCTCA GGTTTTCTGT CCAGCACCTT ACCACCATTT GCCCTCAGAT 
GCAGAAAGAA ATGCTGTGAT ATTTCTTCTC CTTCCAAAAC CTCCACTAGA AAGCATTGGC 
CACATCAAGG TAAAGGGCAA AGGCTTGAAA CGGAAGGTCT TTCACGCTAG CTGTGAATGG 
CTCCTGCCCA GAGACTCAGC CGGGAGAGCC TCCAGCAGCA GCAGCAGCAG CAGCGCAGGC 
GGATGGAACC GACTTAGCCT CACCCATGTC TCCTCGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142860.1
CDS	271..8172
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287374.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287374.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGAAACTG GTGAATTTCC CTCAGAATGT TGCAGTGTTA TGGCTGGGGG AACACTTACA 
GGATGGCACG CAGATGTTGC TACTGTGATG TGGAGACGAA TGCTAGGAAT TTTAGGTGAT 
GTCAACAGCA TCATGGATCC AGAGATTCAT GCCCAGGTTT TTGATTACCT GTGTGAACTG 
TGGCAGAATC TGGCCAAGAT AAGAGACAAT CTTGGTATTT CAACAGATAA CCTCACTTCA 
CCATCTCCAC CAGTTTTAAT CCCACCACTG AGAATTCTAA CACCTTGGCT GTTCAAGGCA 
ACAATGCTGA CTGATAAATA CAAACAAGGG AAGCTACATG CTTATAAACT CATTTGTAAG 
ACAATGAAGC GAAGACAAGA TGTATCTCCA AACAAGGATT TTTTGACTCA TTTCTATAAT 
ATAATGCACT GTGGACTTCT TCATGTTGAT CAGGATATTG TCAATACAAT CATCAAGCAC 
TGCTCTCCTC AGTTCTTTTC ACTTAGTTTG CCTGGTGCCA CCATGCTTAT TATGGATTTC 
ATTGTAGCAG CAGGAAGGGT GGCAGCTTCT TCTTTTCTCA ATGCACCAAG AGTTGAAGCT 
CAAGTTCTTT TAGGATCTCT TGTCTGTTTT CCCAATTTAT ATCATGAACT ACCAGCTCTT 
CACCCAAACA TTCCTGACAT AGCTGTGTCT CAGTTTACAG ATGTTAAGGA GCTCATAATT 
AAAACTGTGT TAAGTTCAGC CAGAGAGGAG CCATCTGGTC CTGCAAGATG TGTTGCTCTT 
TGCAGCCTTG GTATTTGGAT CTGTGAGGAG CTAGTTCATG AATCTCATCA TCCTCAGATC 
AAGGAAGCAT TGAATGTGAT TTGTGTTTCT TTAAAGTTTC CGAATAAGAC AGTAGCTCAA 
GTTGCCTGCA GTATGCTGAA TATGCTGATA CATTATGTGC ATAGACTTCA AGTGTATCAA 
GCTGATTCAC CTTTAAGAAT TATTCAAATT CTGATAGCTA CAATTACCCA TCTGCTACCC 
GGTACAGAAG CTTCATCCTA TGAACAGGAC AAGAGGTTGG TAGTTTCCTT ACTTCTGTGC 
TTATTGGATT GGATAATGGC CTTGCCATTG AAGACCTTGC TGCAGCCTCT TCACGCTACA 
GGAGCAGAAA ATGATAAGAC TGAGAGATCT GTTCTTAATT GTATATATAA GGTTCTACAT 
GGATGTGTCT ATGGATCTCA GTGTTTTAGT AACCCCAAAT ATTTTCCCCT AAGTCTTTCT 
GATTTGGCAT GTGTGGATTA CGATCCATTT ATGCATTTAG AAAGCTTGAA GGAGCCAGAA 
CCACTGCATT CTCCAGACTC AGAGCGTTCT TCTAAACTTC AACCAGTCAC CGAAGTGAAA 
ACTCACATGC AACAAGGATT AATTTCTGTT GCTGCTCGTA CTGTGATAAC GCATCTAGTC 
AACCACTTAG GTCACTATCC TATGAGTGGA GGACCTGCTA TGCTGACTAG TCAAGTGTGT 
GAAAATAATG ACAATCCATA TAGTGAAAGT CCTGAACTTT CTCCTGAACT TTTTGAGAAC 
CCAAACCTTC AATTCTTTGT GTTAAACAAT ACTTCCTTGG TGTCTTGCAT ACAAATCCAA 
GCAGAAGATG ACATGCCTGG GGGAGGACTG TCTGCTGGAC TTGCATCTGC TAATTCAAAT 
GTCAGAATTA TAGTGCGTGA TCTGTCTGGC AAATACTCAT GGGATTCAGC CATTCTGTAT 
GGACCATCAT CCTTATGTGC ATCTTCACAA CAAACATCTT TTGCCCTTTC ATTATCTCAA 
CAAGAAAAAA CGGAAGATCC CCTTTCTTCT TTTGAGCATG TGGAGGACAT ATCTGCAAGG 
GATGGAATTA CACTCCAGGT AAAAAGGAAA TTCAGAGACA CTGTGCCAAC ATGGGATACA 
ATTAGGGATG AAGAAGATGC CCTTGATGAG CTCTTGCAGT ATTTAGGTGT TACAAGCCCT 
GAATGCTTAC AGAGAACTGG AGTCTCACTC AACATCCCTG CTCCTCAACC AGTCTGCATC 
TCAGAGAAGC AGGAGAATGA CGTCATCAAT GCAATTCTGA AACAGCATAC AGAAGAGAGA 
GAGTTTGTTG AAAAACATTT CAATGATTTA AATATGAGGG CTATGGAGCA GGATGAGCCA 
ACATCGCAAA AGCCCCAGTC AGCATTTTAC TACTGCAGAC TACTTCTCAG TATACTGGGA 
ATGAATTCTT GGGATAAAAG AAGGAGCTTT CATCTCCTGA AGAAAAATGA AAAGTTACTT 
CGAGAGCTCA GAAATTTGGA CTCAAGACAG TGCCGAGAGA CCCACAAGAT TGCAGTGTTT 
TATGTTGCTG AGGGACAAGA AGATAAACAC TCCATTCTTA CTAATACTGG GGGAAGTCAA 
GCCTATGAGG ATTTTGTAGC TGGCCTTGGC TGGGAGGTAA ATCTCACAAA CCATTGTGGC 
TTTATGGGAG GACTGCAAAA AAACAAAAGC ACTGGATTGA CCACTCCATA TTTTGCTACC 
TCAACAGTAG AAGTAATGTT CCATGTGTCA ACAAGAATGC CTTCTGATTC AGATGACTCT 
CTAACAAAAA AGCTAAGACA TTTAGGGAAC GATGAAGTAC ACATTGTTTG GTCAGAGCAT 
ACTCGAGATT ATAGAAGAGG AATAATTCCA ACCGAATTTG GTGATGTTCT TATTGTTATC 
TATCCCATGA AAAACCACAT GTTCAGTATC CAGATCATGA AGAAGCCTGA GGTTCCATTT 
TTTGGTCCAC TTTTTGATGG TGCTATTGTG AATGGAAAAA TTCTTCCTAT TATGGTTCGC 
GCAACAGCTA TAAATGCAAG CCGTGCATTG AAATCATTAA TCCCTTTATA CCAAAACTTC 
TATGAGGAGA GAGCACGATA CCTGCAAACA ATTGTTCAGC ATCACCTAGA ACCTACAACT 
TTTGAGGATT TTGCTGCTCA GGTTTTCTGT CCAGCACCTT ACCACCATTT GCCCTCAGAT 
GCAGAAAGAA ATGCTGTGAT ATTTCTTCTC CTTCCAAAAC CTCCACTAGA AAGCATTGGC 
CACATCAAGG TAAAGGGCAA AGGCTTGAAA CGGAAGGTCT TTCACGCTAG CTGTGAATGG 
CTCCTGCCCA GAGACTCAGC CGGGAGAGCC TCCAGCAGCA GCAGCAGCAG CAGCGCAGGC 
GGATGGAACC GACTTAGCCT CACCCATGTC TCCTCGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287375.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142861.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.

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CloneID	OGa36821
Clone ID Related Accession (Same CDS sequence)	XM_015287375.1 , XM_015287375.2
Accession Version	XM_015287375.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAATTT CAGCTTTGGC 
ACAGAGGCAG GGACGCCAGC TTCCACTGAT GCTGATGCAA TTTCAGGACA GCAGCAGAGT 
GCTGAGGAAC AAGAAGTAGC CAGTCTAACT ACACTCCACA TAGATTCGGA AACAAGTAGT 
CTGAATCAGC AACCGTTGTC TGCTGAAGCT GCAACTGTTA CTGGCTCTGA AAGTGCTTCT 
CCAATTCAAT CTGCTCTTGG ATCCCGATCA CAGACACCCT CTCCTGCCAC TCTTCATGCA 
GATCATATTG AGCAGAAGGA TCTGCAACTG GATGAGAAGC TCCACCACTC TGTTTTGCAG 
ACGCCAGATG ACCTAGAAAC TGGTGAATTT CCCTCAGAAT GTTGCAGTGT TATGGCTGGG 
GGAACACTTA CAGGATGGCA CGCAGATGTT GCTACTGTGA TGTGGAGACG AATGCTAGGA 
ATTTTAGGTG ATGTCAACAG CATCATGGAT CCAGAGATTC ATGCCCAGGT TTTTGATTAC 
CTGTGTGAAC TGTGGCAGAA TCTGGCCAAG ATAAGAGACA ATCTTGGTAT TTCAACAGAT 
AACCTCACTT CACCATCTCC ACCAGTTTTA ATCCCACCAC TGAGAATTCT AACACCTTGG 
CTGTTCAAGG CAACAATGCT GACTGATAAA TACAAACAAG GGAAGCTACA TGCTTATAAA 
CTCATTTGTA AGACAATGAA GCGAAGACAA GATGTATCTC CAAACAAGGA TTTTTTGACT 
CATTTCTATA ATATAATGCA CTGTGGACTT CTTCATGTTG ATCAGGATAT TGTCAATACA 
ATCATCAAGC ACTGCTCTCC TCAGTTCTTT TCACTTAGTT TGCCTGGTGC CACCATGCTT 
ATTATGGATT TCATTGTAGC AGCAGGAAGG GTGGCAGCTT CTTCTTTTCT CAATGCACCA 
AGAGTTGAAG CTCAAGTTCT TTTAGGATCT CTTGTCTGTT TTCCCAATTT ATATCATGAA 
CTACCAGCTC TTCACCCAAA CATTCCTGAC ATAGCTGTGT CTCAGTTTAC AGATGTTAAG 
GAGCTCATAA TTAAAACTGT GTTAAGTTCA GCCAGAGAGG AGCCATCTGG TCCTGCAAGA 
TGTGTTGCTC TTTGCAGCCT TGGTATTTGG ATCTGTGAGG AGCTAGTTCA TGAATCTCAT 
CATCCTCAGA TCAAGGAAGC ATTGAATGTG ATTTGTGTTT CTTTAAAGTT TCCGAATAAG 
ACAGTAGCTC AAGTTGCCTG CAGTATGCTG AATATGCTGA TACATTATGT GCATAGACTT 
CAAGTGTATC AAGCTGATTC ACCTTTAAGA ATTATTCAAA TTCTGATAGC TACAATTACC 
CATCTGCTAC CCGGTACAGA AGCTTCATCC TATGAACAGG ACAAGAGGTT GGTAGTTTCC 
TTACTTCTGT GCTTATTGGA TTGGATAATG GCCTTGCCAT TGAAGACCTT GCTGCAGCCT 
CTTCACGCTA CAGGAGCAGA AAATGATAAG ACTGAGAGAT CTGTTCTTAA TTGTATATAT 
AAGGTTCTAC ATGGATGTGT CTATGGATCT CAGTGTTTTA GTAACCCCAA ATATTTTCCC 
CTAAGTCTTT CTGATTTGGC ATGTGTGGAT TACGATCCAT TTATGCATTT AGAAAGCTTG 
AAGGAGCCAG AACCACTGCA TTCTCCAGAC TCAGAGCGTT CTTCTAAACT TCAACCAGTC 
ACCGAAGTGA AAACTCACAT GCAACAAGGA TTAATTTCTG TTGCTGCTCG TACTGTGATA 
ACGCATCTAG TCAACCACTT AGGTCACTAT CCTATGAGTG GAGGACCTGC TATGCTGACT 
AGTCAAGTGT GTGAAAATAA TGACAATCCA TATAGTGAAA GTCCTGAACT TTCTCCTGAA 
CTTTTTGAGA ACCCAAACCT TCAATTCTTT GTGTTAAACA ATACTTCCTT GGTGTCTTGC 
ATACAAATCC AAGCAGAAGA TGACATGCCT GGGGGAGGAC TGTCTGCTGG ACTTGCATCT 
GCTAATTCAA ATGTCAGAAT TATAGTGCGT GATCTGTCTG GCAAATACTC ATGGGATTCA 
GCCATTCTGT ATGGACCATC ATCCTTATGT GCATCTTCAC AACAAACATC TTTTGCCCTT 
TCATTATCTC AACAAGAAAA AACGGAAGAT CCCCTTTCTT CTTTTGAGCA TGTGGAGGAC 
ATATCTGCAA GGGATGGAAT TACACTCCAG GTAAAAAGGA AATTCAGAGA CACTGTGCCA 
ACATGGGATA CAATTAGGGA TGAAGAAGAT GCCCTTGATG AGCTCTTGCA GTATTTAGGT 
GTTACAAGCC CTGAATGCTT ACAGAGAACT GGAGTCTCAC TCAACATCCC TGCTCCTCAA 
CCAGTCTGCA TCTCAGAGAA GCAGGAGAAT GACGTCATCA ATGCAATTCT GAAACAGCAT 
ACAGAAGAGA GAGAGTTTGT TGAAAAACAT TTCAATGATT TAAATATGAG GGCTATGGAG 
CAGGATGAGC CAACATCGCA AAAGCCCCAG TCAGCATTTT ACTACTGCAG ACTACTTCTC 
AGTATACTGG GAATGAATTC TTGGGATAAA AGAAGGAGCT TTCATCTCCT GAAGAAAAAT 
GAAAAGTTAC TTCGAGAGCT CAGAAATTTG GACTCAAGAC AGTGCCGAGA GACCCACAAG 
ATTGCAGTGT TTTATGTTGC TGAGGGACAA GAAGATAAAC ACTCCATTCT TACTAATACT 
GGGGGAAGTC AAGCCTATGA GGATTTTGTA GCTGGCCTTG GCTGGGAGGT AAATCTCACA 
AACCATTGTG GCTTTATGGG AGGACTGCAA AAAAACAAAA GCACTGGATT GACCACTCCA 
TATTTTGCTA CCTCAACAGT AGAAGTAATG TTCCATGTGT CAACAAGAAT GCCTTCTGAT 
TCAGATGACT CTCTAACAAA AAAGCTAAGA CATTTAGGGA ACGATGAAGT ACACATTGTT 
TGGTCAGAGC ATACTCGAGA TTATAGAAGA GGAATAATTC CAACCGAATT TGGTGATGTT 
CTTATTGTTA TCTATCCCAT GAAAAACCAC ATGTTCAGTA TCCAGATCAT GAAGAAGCCT 
GAGGTTCCAT TTTTTGGTCC ACTTTTTGAT GGTGCTATTG TGAATGGAAA AATTCTTCCT 
ATTATGGTTC GCGCAACAGC TATAAATGCA AGCCGTGCAT TGAAATCATT AATCCCTTTA 
TACCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTTCA GCATCACCTA 
GAACCTACAA CTTTTGAGGA TTTTGCTGCT CAGGTTTTCT GTCCAGCACC TTACCACCAT 
TTGCCCTCAG ATGCAGAAAG AAATGCTGTG ATATTTCTTC TCCTTCCAAA ACCTCCACTA 
GAAAGCATTG GCCACATCAA GGTAAAGGGC AAAGGCTTGA AACGGAAGGT CTTTCACGCT 
AGCTGTGAAT GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC 
AGCAGCGCAG GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142861.1
CDS	270..8123
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287375.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287375.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAATTT CAGCTTTGGC 
ACAGAGGCAG GGACGCCAGC TTCCACTGAT GCTGATGCAA TTTCAGGACA GCAGCAGAGT 
GCTGAGGAAC AAGAAGTAGC CAGTCTAACT ACACTCCACA TAGATTCGGA AACAAGTAGT 
CTGAATCAGC AACCGTTGTC TGCTGAAGCT GCAACTGTTA CTGGCTCTGA AAGTGCTTCT 
CCAATTCAAT CTGCTCTTGG ATCCCGATCA CAGACACCCT CTCCTGCCAC TCTTCATGCA 
GATCATATTG AGCAGAAGGA TCTGCAACTG GATGAGAAGC TCCACCACTC TGTTTTGCAG 
ACGCCAGATG ACCTAGAAAC TGGTGAATTT CCCTCAGAAT GTTGCAGTGT TATGGCTGGG 
GGAACACTTA CAGGATGGCA CGCAGATGTT GCTACTGTGA TGTGGAGACG AATGCTAGGA 
ATTTTAGGTG ATGTCAACAG CATCATGGAT CCAGAGATTC ATGCCCAGGT TTTTGATTAC 
CTGTGTGAAC TGTGGCAGAA TCTGGCCAAG ATAAGAGACA ATCTTGGTAT TTCAACAGAT 
AACCTCACTT CACCATCTCC ACCAGTTTTA ATCCCACCAC TGAGAATTCT AACACCTTGG 
CTGTTCAAGG CAACAATGCT GACTGATAAA TACAAACAAG GGAAGCTACA TGCTTATAAA 
CTCATTTGTA AGACAATGAA GCGAAGACAA GATGTATCTC CAAACAAGGA TTTTTTGACT 
CATTTCTATA ATATAATGCA CTGTGGACTT CTTCATGTTG ATCAGGATAT TGTCAATACA 
ATCATCAAGC ACTGCTCTCC TCAGTTCTTT TCACTTAGTT TGCCTGGTGC CACCATGCTT 
ATTATGGATT TCATTGTAGC AGCAGGAAGG GTGGCAGCTT CTTCTTTTCT CAATGCACCA 
AGAGTTGAAG CTCAAGTTCT TTTAGGATCT CTTGTCTGTT TTCCCAATTT ATATCATGAA 
CTACCAGCTC TTCACCCAAA CATTCCTGAC ATAGCTGTGT CTCAGTTTAC AGATGTTAAG 
GAGCTCATAA TTAAAACTGT GTTAAGTTCA GCCAGAGAGG AGCCATCTGG TCCTGCAAGA 
TGTGTTGCTC TTTGCAGCCT TGGTATTTGG ATCTGTGAGG AGCTAGTTCA TGAATCTCAT 
CATCCTCAGA TCAAGGAAGC ATTGAATGTG ATTTGTGTTT CTTTAAAGTT TCCGAATAAG 
ACAGTAGCTC AAGTTGCCTG CAGTATGCTG AATATGCTGA TACATTATGT GCATAGACTT 
CAAGTGTATC AAGCTGATTC ACCTTTAAGA ATTATTCAAA TTCTGATAGC TACAATTACC 
CATCTGCTAC CCGGTACAGA AGCTTCATCC TATGAACAGG ACAAGAGGTT GGTAGTTTCC 
TTACTTCTGT GCTTATTGGA TTGGATAATG GCCTTGCCAT TGAAGACCTT GCTGCAGCCT 
CTTCACGCTA CAGGAGCAGA AAATGATAAG ACTGAGAGAT CTGTTCTTAA TTGTATATAT 
AAGGTTCTAC ATGGATGTGT CTATGGATCT CAGTGTTTTA GTAACCCCAA ATATTTTCCC 
CTAAGTCTTT CTGATTTGGC ATGTGTGGAT TACGATCCAT TTATGCATTT AGAAAGCTTG 
AAGGAGCCAG AACCACTGCA TTCTCCAGAC TCAGAGCGTT CTTCTAAACT TCAACCAGTC 
ACCGAAGTGA AAACTCACAT GCAACAAGGA TTAATTTCTG TTGCTGCTCG TACTGTGATA 
ACGCATCTAG TCAACCACTT AGGTCACTAT CCTATGAGTG GAGGACCTGC TATGCTGACT 
AGTCAAGTGT GTGAAAATAA TGACAATCCA TATAGTGAAA GTCCTGAACT TTCTCCTGAA 
CTTTTTGAGA ACCCAAACCT TCAATTCTTT GTGTTAAACA ATACTTCCTT GGTGTCTTGC 
ATACAAATCC AAGCAGAAGA TGACATGCCT GGGGGAGGAC TGTCTGCTGG ACTTGCATCT 
GCTAATTCAA ATGTCAGAAT TATAGTGCGT GATCTGTCTG GCAAATACTC ATGGGATTCA 
GCCATTCTGT ATGGACCATC ATCCTTATGT GCATCTTCAC AACAAACATC TTTTGCCCTT 
TCATTATCTC AACAAGAAAA AACGGAAGAT CCCCTTTCTT CTTTTGAGCA TGTGGAGGAC 
ATATCTGCAA GGGATGGAAT TACACTCCAG GTAAAAAGGA AATTCAGAGA CACTGTGCCA 
ACATGGGATA CAATTAGGGA TGAAGAAGAT GCCCTTGATG AGCTCTTGCA GTATTTAGGT 
GTTACAAGCC CTGAATGCTT ACAGAGAACT GGAGTCTCAC TCAACATCCC TGCTCCTCAA 
CCAGTCTGCA TCTCAGAGAA GCAGGAGAAT GACGTCATCA ATGCAATTCT GAAACAGCAT 
ACAGAAGAGA GAGAGTTTGT TGAAAAACAT TTCAATGATT TAAATATGAG GGCTATGGAG 
CAGGATGAGC CAACATCGCA AAAGCCCCAG TCAGCATTTT ACTACTGCAG ACTACTTCTC 
AGTATACTGG GAATGAATTC TTGGGATAAA AGAAGGAGCT TTCATCTCCT GAAGAAAAAT 
GAAAAGTTAC TTCGAGAGCT CAGAAATTTG GACTCAAGAC AGTGCCGAGA GACCCACAAG 
ATTGCAGTGT TTTATGTTGC TGAGGGACAA GAAGATAAAC ACTCCATTCT TACTAATACT 
GGGGGAAGTC AAGCCTATGA GGATTTTGTA GCTGGCCTTG GCTGGGAGGT AAATCTCACA 
AACCATTGTG GCTTTATGGG AGGACTGCAA AAAAACAAAA GCACTGGATT GACCACTCCA 
TATTTTGCTA CCTCAACAGT AGAAGTAATG TTCCATGTGT CAACAAGAAT GCCTTCTGAT 
TCAGATGACT CTCTAACAAA AAAGCTAAGA CATTTAGGGA ACGATGAAGT ACACATTGTT 
TGGTCAGAGC ATACTCGAGA TTATAGAAGA GGAATAATTC CAACCGAATT TGGTGATGTT 
CTTATTGTTA TCTATCCCAT GAAAAACCAC ATGTTCAGTA TCCAGATCAT GAAGAAGCCT 
GAGGTTCCAT TTTTTGGTCC ACTTTTTGAT GGTGCTATTG TGAATGGAAA AATTCTTCCT 
ATTATGGTTC GCGCAACAGC TATAAATGCA AGCCGTGCAT TGAAATCATT AATCCCTTTA 
TACCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTTCA GCATCACCTA 
GAACCTACAA CTTTTGAGGA TTTTGCTGCT CAGGTTTTCT GTCCAGCACC TTACCACCAT 
TTGCCCTCAG ATGCAGAAAG AAATGCTGTG ATATTTCTTC TCCTTCCAAA ACCTCCACTA 
GAAAGCATTG GCCACATCAA GGTAAAGGGC AAAGGCTTGA AACGGAAGGT CTTTCACGCT 
AGCTGTGAAT GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC 
AGCAGCGCAG GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287375.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142861.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X7, mRNA.

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CloneID	OGa36821
Clone ID Related Accession (Same CDS sequence)	XM_015287375.1 , XM_015287375.2
Accession Version	XM_015287375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAATTT CAGCTTTGGC 
ACAGAGGCAG GGACGCCAGC TTCCACTGAT GCTGATGCAA TTTCAGGACA GCAGCAGAGT 
GCTGAGGAAC AAGAAGTAGC CAGTCTAACT ACACTCCACA TAGATTCGGA AACAAGTAGT 
CTGAATCAGC AACCGTTGTC TGCTGAAGCT GCAACTGTTA CTGGCTCTGA AAGTGCTTCT 
CCAATTCAAT CTGCTCTTGG ATCCCGATCA CAGACACCCT CTCCTGCCAC TCTTCATGCA 
GATCATATTG AGCAGAAGGA TCTGCAACTG GATGAGAAGC TCCACCACTC TGTTTTGCAG 
ACGCCAGATG ACCTAGAAAC TGGTGAATTT CCCTCAGAAT GTTGCAGTGT TATGGCTGGG 
GGAACACTTA CAGGATGGCA CGCAGATGTT GCTACTGTGA TGTGGAGACG AATGCTAGGA 
ATTTTAGGTG ATGTCAACAG CATCATGGAT CCAGAGATTC ATGCCCAGGT TTTTGATTAC 
CTGTGTGAAC TGTGGCAGAA TCTGGCCAAG ATAAGAGACA ATCTTGGTAT TTCAACAGAT 
AACCTCACTT CACCATCTCC ACCAGTTTTA ATCCCACCAC TGAGAATTCT AACACCTTGG 
CTGTTCAAGG CAACAATGCT GACTGATAAA TACAAACAAG GGAAGCTACA TGCTTATAAA 
CTCATTTGTA AGACAATGAA GCGAAGACAA GATGTATCTC CAAACAAGGA TTTTTTGACT 
CATTTCTATA ATATAATGCA CTGTGGACTT CTTCATGTTG ATCAGGATAT TGTCAATACA 
ATCATCAAGC ACTGCTCTCC TCAGTTCTTT TCACTTAGTT TGCCTGGTGC CACCATGCTT 
ATTATGGATT TCATTGTAGC AGCAGGAAGG GTGGCAGCTT CTTCTTTTCT CAATGCACCA 
AGAGTTGAAG CTCAAGTTCT TTTAGGATCT CTTGTCTGTT TTCCCAATTT ATATCATGAA 
CTACCAGCTC TTCACCCAAA CATTCCTGAC ATAGCTGTGT CTCAGTTTAC AGATGTTAAG 
GAGCTCATAA TTAAAACTGT GTTAAGTTCA GCCAGAGAGG AGCCATCTGG TCCTGCAAGA 
TGTGTTGCTC TTTGCAGCCT TGGTATTTGG ATCTGTGAGG AGCTAGTTCA TGAATCTCAT 
CATCCTCAGA TCAAGGAAGC ATTGAATGTG ATTTGTGTTT CTTTAAAGTT TCCGAATAAG 
ACAGTAGCTC AAGTTGCCTG CAGTATGCTG AATATGCTGA TACATTATGT GCATAGACTT 
CAAGTGTATC AAGCTGATTC ACCTTTAAGA ATTATTCAAA TTCTGATAGC TACAATTACC 
CATCTGCTAC CCGGTACAGA AGCTTCATCC TATGAACAGG ACAAGAGGTT GGTAGTTTCC 
TTACTTCTGT GCTTATTGGA TTGGATAATG GCCTTGCCAT TGAAGACCTT GCTGCAGCCT 
CTTCACGCTA CAGGAGCAGA AAATGATAAG ACTGAGAGAT CTGTTCTTAA TTGTATATAT 
AAGGTTCTAC ATGGATGTGT CTATGGATCT CAGTGTTTTA GTAACCCCAA ATATTTTCCC 
CTAAGTCTTT CTGATTTGGC ATGTGTGGAT TACGATCCAT TTATGCATTT AGAAAGCTTG 
AAGGAGCCAG AACCACTGCA TTCTCCAGAC TCAGAGCGTT CTTCTAAACT TCAACCAGTC 
ACCGAAGTGA AAACTCACAT GCAACAAGGA TTAATTTCTG TTGCTGCTCG TACTGTGATA 
ACGCATCTAG TCAACCACTT AGGTCACTAT CCTATGAGTG GAGGACCTGC TATGCTGACT 
AGTCAAGTGT GTGAAAATAA TGACAATCCA TATAGTGAAA GTCCTGAACT TTCTCCTGAA 
CTTTTTGAGA ACCCAAACCT TCAATTCTTT GTGTTAAACA ATACTTCCTT GGTGTCTTGC 
ATACAAATCC AAGCAGAAGA TGACATGCCT GGGGGAGGAC TGTCTGCTGG ACTTGCATCT 
GCTAATTCAA ATGTCAGAAT TATAGTGCGT GATCTGTCTG GCAAATACTC ATGGGATTCA 
GCCATTCTGT ATGGACCATC ATCCTTATGT GCATCTTCAC AACAAACATC TTTTGCCCTT 
TCATTATCTC AACAAGAAAA AACGGAAGAT CCCCTTTCTT CTTTTGAGCA TGTGGAGGAC 
ATATCTGCAA GGGATGGAAT TACACTCCAG GTAAAAAGGA AATTCAGAGA CACTGTGCCA 
ACATGGGATA CAATTAGGGA TGAAGAAGAT GCCCTTGATG AGCTCTTGCA GTATTTAGGT 
GTTACAAGCC CTGAATGCTT ACAGAGAACT GGAGTCTCAC TCAACATCCC TGCTCCTCAA 
CCAGTCTGCA TCTCAGAGAA GCAGGAGAAT GACGTCATCA ATGCAATTCT GAAACAGCAT 
ACAGAAGAGA GAGAGTTTGT TGAAAAACAT TTCAATGATT TAAATATGAG GGCTATGGAG 
CAGGATGAGC CAACATCGCA AAAGCCCCAG TCAGCATTTT ACTACTGCAG ACTACTTCTC 
AGTATACTGG GAATGAATTC TTGGGATAAA AGAAGGAGCT TTCATCTCCT GAAGAAAAAT 
GAAAAGTTAC TTCGAGAGCT CAGAAATTTG GACTCAAGAC AGTGCCGAGA GACCCACAAG 
ATTGCAGTGT TTTATGTTGC TGAGGGACAA GAAGATAAAC ACTCCATTCT TACTAATACT 
GGGGGAAGTC AAGCCTATGA GGATTTTGTA GCTGGCCTTG GCTGGGAGGT AAATCTCACA 
AACCATTGTG GCTTTATGGG AGGACTGCAA AAAAACAAAA GCACTGGATT GACCACTCCA 
TATTTTGCTA CCTCAACAGT AGAAGTAATG TTCCATGTGT CAACAAGAAT GCCTTCTGAT 
TCAGATGACT CTCTAACAAA AAAGCTAAGA CATTTAGGGA ACGATGAAGT ACACATTGTT 
TGGTCAGAGC ATACTCGAGA TTATAGAAGA GGAATAATTC CAACCGAATT TGGTGATGTT 
CTTATTGTTA TCTATCCCAT GAAAAACCAC ATGTTCAGTA TCCAGATCAT GAAGAAGCCT 
GAGGTTCCAT TTTTTGGTCC ACTTTTTGAT GGTGCTATTG TGAATGGAAA AATTCTTCCT 
ATTATGGTTC GCGCAACAGC TATAAATGCA AGCCGTGCAT TGAAATCATT AATCCCTTTA 
TACCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTTCA GCATCACCTA 
GAACCTACAA CTTTTGAGGA TTTTGCTGCT CAGGTTTTCT GTCCAGCACC TTACCACCAT 
TTGCCCTCAG ATGCAGAAAG AAATGCTGTG ATATTTCTTC TCCTTCCAAA ACCTCCACTA 
GAAAGCATTG GCCACATCAA GGTAAAGGGC AAAGGCTTGA AACGGAAGGT CTTTCACGCT 
AGCTGTGAAT GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC 
AGCAGCGCAG GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142861.1
CDS	268..8121
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287375.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287375.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAATTT CAGCTTTGGC 
ACAGAGGCAG GGACGCCAGC TTCCACTGAT GCTGATGCAA TTTCAGGACA GCAGCAGAGT 
GCTGAGGAAC AAGAAGTAGC CAGTCTAACT ACACTCCACA TAGATTCGGA AACAAGTAGT 
CTGAATCAGC AACCGTTGTC TGCTGAAGCT GCAACTGTTA CTGGCTCTGA AAGTGCTTCT 
CCAATTCAAT CTGCTCTTGG ATCCCGATCA CAGACACCCT CTCCTGCCAC TCTTCATGCA 
GATCATATTG AGCAGAAGGA TCTGCAACTG GATGAGAAGC TCCACCACTC TGTTTTGCAG 
ACGCCAGATG ACCTAGAAAC TGGTGAATTT CCCTCAGAAT GTTGCAGTGT TATGGCTGGG 
GGAACACTTA CAGGATGGCA CGCAGATGTT GCTACTGTGA TGTGGAGACG AATGCTAGGA 
ATTTTAGGTG ATGTCAACAG CATCATGGAT CCAGAGATTC ATGCCCAGGT TTTTGATTAC 
CTGTGTGAAC TGTGGCAGAA TCTGGCCAAG ATAAGAGACA ATCTTGGTAT TTCAACAGAT 
AACCTCACTT CACCATCTCC ACCAGTTTTA ATCCCACCAC TGAGAATTCT AACACCTTGG 
CTGTTCAAGG CAACAATGCT GACTGATAAA TACAAACAAG GGAAGCTACA TGCTTATAAA 
CTCATTTGTA AGACAATGAA GCGAAGACAA GATGTATCTC CAAACAAGGA TTTTTTGACT 
CATTTCTATA ATATAATGCA CTGTGGACTT CTTCATGTTG ATCAGGATAT TGTCAATACA 
ATCATCAAGC ACTGCTCTCC TCAGTTCTTT TCACTTAGTT TGCCTGGTGC CACCATGCTT 
ATTATGGATT TCATTGTAGC AGCAGGAAGG GTGGCAGCTT CTTCTTTTCT CAATGCACCA 
AGAGTTGAAG CTCAAGTTCT TTTAGGATCT CTTGTCTGTT TTCCCAATTT ATATCATGAA 
CTACCAGCTC TTCACCCAAA CATTCCTGAC ATAGCTGTGT CTCAGTTTAC AGATGTTAAG 
GAGCTCATAA TTAAAACTGT GTTAAGTTCA GCCAGAGAGG AGCCATCTGG TCCTGCAAGA 
TGTGTTGCTC TTTGCAGCCT TGGTATTTGG ATCTGTGAGG AGCTAGTTCA TGAATCTCAT 
CATCCTCAGA TCAAGGAAGC ATTGAATGTG ATTTGTGTTT CTTTAAAGTT TCCGAATAAG 
ACAGTAGCTC AAGTTGCCTG CAGTATGCTG AATATGCTGA TACATTATGT GCATAGACTT 
CAAGTGTATC AAGCTGATTC ACCTTTAAGA ATTATTCAAA TTCTGATAGC TACAATTACC 
CATCTGCTAC CCGGTACAGA AGCTTCATCC TATGAACAGG ACAAGAGGTT GGTAGTTTCC 
TTACTTCTGT GCTTATTGGA TTGGATAATG GCCTTGCCAT TGAAGACCTT GCTGCAGCCT 
CTTCACGCTA CAGGAGCAGA AAATGATAAG ACTGAGAGAT CTGTTCTTAA TTGTATATAT 
AAGGTTCTAC ATGGATGTGT CTATGGATCT CAGTGTTTTA GTAACCCCAA ATATTTTCCC 
CTAAGTCTTT CTGATTTGGC ATGTGTGGAT TACGATCCAT TTATGCATTT AGAAAGCTTG 
AAGGAGCCAG AACCACTGCA TTCTCCAGAC TCAGAGCGTT CTTCTAAACT TCAACCAGTC 
ACCGAAGTGA AAACTCACAT GCAACAAGGA TTAATTTCTG TTGCTGCTCG TACTGTGATA 
ACGCATCTAG TCAACCACTT AGGTCACTAT CCTATGAGTG GAGGACCTGC TATGCTGACT 
AGTCAAGTGT GTGAAAATAA TGACAATCCA TATAGTGAAA GTCCTGAACT TTCTCCTGAA 
CTTTTTGAGA ACCCAAACCT TCAATTCTTT GTGTTAAACA ATACTTCCTT GGTGTCTTGC 
ATACAAATCC AAGCAGAAGA TGACATGCCT GGGGGAGGAC TGTCTGCTGG ACTTGCATCT 
GCTAATTCAA ATGTCAGAAT TATAGTGCGT GATCTGTCTG GCAAATACTC ATGGGATTCA 
GCCATTCTGT ATGGACCATC ATCCTTATGT GCATCTTCAC AACAAACATC TTTTGCCCTT 
TCATTATCTC AACAAGAAAA AACGGAAGAT CCCCTTTCTT CTTTTGAGCA TGTGGAGGAC 
ATATCTGCAA GGGATGGAAT TACACTCCAG GTAAAAAGGA AATTCAGAGA CACTGTGCCA 
ACATGGGATA CAATTAGGGA TGAAGAAGAT GCCCTTGATG AGCTCTTGCA GTATTTAGGT 
GTTACAAGCC CTGAATGCTT ACAGAGAACT GGAGTCTCAC TCAACATCCC TGCTCCTCAA 
CCAGTCTGCA TCTCAGAGAA GCAGGAGAAT GACGTCATCA ATGCAATTCT GAAACAGCAT 
ACAGAAGAGA GAGAGTTTGT TGAAAAACAT TTCAATGATT TAAATATGAG GGCTATGGAG 
CAGGATGAGC CAACATCGCA AAAGCCCCAG TCAGCATTTT ACTACTGCAG ACTACTTCTC 
AGTATACTGG GAATGAATTC TTGGGATAAA AGAAGGAGCT TTCATCTCCT GAAGAAAAAT 
GAAAAGTTAC TTCGAGAGCT CAGAAATTTG GACTCAAGAC AGTGCCGAGA GACCCACAAG 
ATTGCAGTGT TTTATGTTGC TGAGGGACAA GAAGATAAAC ACTCCATTCT TACTAATACT 
GGGGGAAGTC AAGCCTATGA GGATTTTGTA GCTGGCCTTG GCTGGGAGGT AAATCTCACA 
AACCATTGTG GCTTTATGGG AGGACTGCAA AAAAACAAAA GCACTGGATT GACCACTCCA 
TATTTTGCTA CCTCAACAGT AGAAGTAATG TTCCATGTGT CAACAAGAAT GCCTTCTGAT 
TCAGATGACT CTCTAACAAA AAAGCTAAGA CATTTAGGGA ACGATGAAGT ACACATTGTT 
TGGTCAGAGC ATACTCGAGA TTATAGAAGA GGAATAATTC CAACCGAATT TGGTGATGTT 
CTTATTGTTA TCTATCCCAT GAAAAACCAC ATGTTCAGTA TCCAGATCAT GAAGAAGCCT 
GAGGTTCCAT TTTTTGGTCC ACTTTTTGAT GGTGCTATTG TGAATGGAAA AATTCTTCCT 
ATTATGGTTC GCGCAACAGC TATAAATGCA AGCCGTGCAT TGAAATCATT AATCCCTTTA 
TACCAAAACT TCTATGAGGA GAGAGCACGA TACCTGCAAA CAATTGTTCA GCATCACCTA 
GAACCTACAA CTTTTGAGGA TTTTGCTGCT CAGGTTTTCT GTCCAGCACC TTACCACCAT 
TTGCCCTCAG ATGCAGAAAG AAATGCTGTG ATATTTCTTC TCCTTCCAAA ACCTCCACTA 
GAAAGCATTG GCCACATCAA GGTAAAGGGC AAAGGCTTGA AACGGAAGGT CTTTCACGCT 
AGCTGTGAAT GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC 
AGCAGCGCAG GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287377.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142863.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.

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CloneID	OGa36822
Clone ID Related Accession (Same CDS sequence)	XM_015287377.1 , XM_015287377.2 , XM_015287376.1 , XM_025150741.1 , XM_015287376.2
Accession Version	XM_015287377.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142863.1
CDS	270..8114
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_015287377.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287377.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287377.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142863.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X9, mRNA.

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CloneID	OGa36822
Clone ID Related Accession (Same CDS sequence)	XM_015287377.1 , XM_015287377.2 , XM_015287376.1 , XM_025150741.1 , XM_015287376.2
Accession Version	XM_015287377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142863.1
CDS	269..8113
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_015287377.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287377.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287376.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142862.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.

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CloneID	OGa36822
Clone ID Related Accession (Same CDS sequence)	XM_015287377.1 , XM_015287377.2 , XM_015287376.1 , XM_025150741.1 , XM_015287376.2
Accession Version	XM_015287376.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142862.1
CDS	270..8114
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_015287376.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287376.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_025150741.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006509.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X10, mRNA.

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CloneID	OGa36822
Clone ID Related Accession (Same CDS sequence)	XM_015287377.1 , XM_015287377.2 , XM_015287376.1 , XM_025150741.1 , XM_015287376.2
Accession Version	XM_025150741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006509.1
CDS	269..8113
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_025150741.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150741.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287376.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142862.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X8, mRNA.

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CloneID	OGa36822
Clone ID Related Accession (Same CDS sequence)	XM_015287377.1 , XM_015287377.2 , XM_015287376.1 , XM_025150741.1 , XM_015287376.2
Accession Version	XM_015287376.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142862.1
CDS	268..8112
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDADH

Target ORF information:

RefSeq Version	XM_015287376.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287376.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGATC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287378.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142864.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.

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CloneID	OGa36823
Clone ID Related Accession (Same CDS sequence)	XM_015287378.1 , XM_015287378.2
Accession Version	XM_015287378.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AGTACACTTA CAGCGACTAA GCAGAATACA 
GGACATGCAA TAGGAATGTC TTCTCCAGAA GTTCAGTCGC AGTTTTGTGA CAGAAATGAG 
AATGTCCATA ATAGCCAATT ACTTTCTGCT TTTACATCTT ATGTAAATTG TGCAACAGTG 
TGTTGGAAGC ATAGGAGATG TACATACAAG TTTTACATGG AGGATTTTGG AGATTTCAGC 
TTTGGCACAG AGGCAGGGAC GCCAGCTTCC ACTGATGCTG ATGCAATTTC AGGACAGCAG 
CAGAGTGCTG AGGAACAAGA AGTAGCCAGT CTAACTACAC TCCACATAGA TTCGGAAACA 
AGTAGTCTGA ATCAGCAACC GTTGTCTGCT GAAGCTGCAA CTGTTACTGG CTCTGAAAGT 
GCTTCTCCAA TTCAATCTGC TCTTGGATCC CGATCACAGA CACCCTCTCC TGCCACTCTT 
CATGCAGATC ATATTGAGCA GAAGGATCTG CAACTGGATG AGAAGCTCCA CCACTCTGTT 
TTGCAGACGC CAGATGACCT AGAAACTGGT GAATTTCCCT CAGAATGTTG CAGTGTTATG 
GCTGGGGGAA CACTTACAGG ATGGCACGCA GATGTTGCTA CTGTGATGTG GAGACGAATG 
CTAGGAATTT TAGGTGATGT CAACAGCATC ATGGATCCAG AGATTCATGC CCAGGTTTTT 
GATTACCTGT GTGAACTGTG GCAGAATCTG GCCAAGATAA GAGACAATCT TGGTATTTCA 
ACAGATAACC TCACTTCACC ATCTCCACCA GTTTTAATCC CACCACTGAG AATTCTAACA 
CCTTGGCTGT TCAAGGCAAC AATGCTGACT GATAAATACA AACAAGGGAA GCTACATGCT 
TATAAACTCA TTTGTAAGAC AATGAAGCGA AGACAAGATG TATCTCCAAA CAAGGATTTT 
TTGACTCATT TCTATAATAT AATGCACTGT GGACTTCTTC ATGTTGATCA GGATATTGTC 
AATACAATCA TCAAGCACTG CTCTCCTCAG TTCTTTTCAC TTAGTTTGCC TGGTGCCACC 
ATGCTTATTA TGGATTTCAT TGTAGCAGCA GGAAGGGTGG CAGCTTCTTC TTTTCTCAAT 
GCACCAAGAG TTGAAGCTCA AGTTCTTTTA GGATCTCTTG TCTGTTTTCC CAATTTATAT 
CATGAACTAC CAGCTCTTCA CCCAAACATT CCTGACATAG CTGTGTCTCA GTTTACAGAT 
GTTAAGGAGC TCATAATTAA AACTGTGTTA AGTTCAGCCA GAGAGGAGCC ATCTGGTCCT 
GCAAGATGTG TTGCTCTTTG CAGCCTTGGT ATTTGGATCT GTGAGGAGCT AGTTCATGAA 
TCTCATCATC CTCAGATCAA GGAAGCATTG AATGTGATTT GTGTTTCTTT AAAGTTTCCG 
AATAAGACAG TAGCTCAAGT TGCCTGCAGT ATGCTGAATA TGCTGATACA TTATGTGCAT 
AGACTTCAAG TGTATCAAGC TGATTCACCT TTAAGAATTA TTCAAATTCT GATAGCTACA 
ATTACCCATC TGCTACCCGG TACAGAAGCT TCATCCTATG AACAGGACAA GAGGTTGGTA 
GTTTCCTTAC TTCTGTGCTT ATTGGATTGG ATAATGGCCT TGCCATTGAA GACCTTGCTG 
CAGCCTCTTC ACGCTACAGG AGCAGAAAAT GATAAGACTG AGAGATCTGT TCTTAATTGT 
ATATATAAGG TTCTACATGG ATGTGTCTAT GGATCTCAGT GTTTTAGTAA CCCCAAATAT 
TTTCCCCTAA GTCTTTCTGA TTTGGCATGT GTGGATTACG ATCCATTTAT GCATTTAGAA 
AGCTTGAAGG AGCCAGAACC ACTGCATTCT CCAGACTCAG AGCGTTCTTC TAAACTTCAA 
CCAGTCACCG AAGTGAAAAC TCACATGCAA CAAGGATTAA TTTCTGTTGC TGCTCGTACT 
GTGATAACGC ATCTAGTCAA CCACTTAGGT CACTATCCTA TGAGTGGAGG ACCTGCTATG 
CTGACTAGTC AAGTGTGTGA AAATAATGAC AATCCATATA GTGAAAGTCC TGAACTTTCT 
CCTGAACTTT TTGAGAACCC AAACCTTCAA TTCTTTGTGT TAAACAATAC TTCCTTGGTG 
TCTTGCATAC AAATCCAAGC AGAAGATGAC ATGCCTGGGG GAGGACTGTC TGCTGGACTT 
GCATCTGCTA ATTCAAATGT CAGAATTATA GTGCGTGATC TGTCTGGCAA ATACTCATGG 
GATTCAGCCA TTCTGTATGG ACCATCATCC TTATGTGCAT CTTCACAACA AACATCTTTT 
GCCCTTTCAT TATCTCAACA AGAAAAAACG GAAGATCCCC TTTCTTCTTT TGAGCATGTG 
GAGGACATAT CTGCAAGGGA TGGAATTACA CTCCAGGTAA AAAGGAAATT CAGAGACACT 
GTGCCAACAT GGGATACAAT TAGGGATGAA GAAGATGCCC TTGATGAGCT CTTGCAGTAT 
TTAGGTGTTA CAAGCCCTGA ATGCTTACAG AGAACTGGAG TCTCACTCAA CATCCCTGCT 
CCTCAACCAG TCTGCATCTC AGAGAAGCAG GAGAATGACG TCATCAATGC AATTCTGAAA 
CAGCATACAG AAGAGAGAGA GTTTGTTGAA AAACATTTCA ATGATTTAAA TATGAGGGCT 
ATGGAGCAGG ATGAGCCAAC ATCGCAAAAG CCCCAGTCAG CATTTTACTA CTGCAGACTA 
CTTCTCAGTA TACTGGGAAT GAATTCTTGG GATAAAAGAA GGAGCTTTCA TCTCCTGAAG 
AAAAATGAAA AGTTACTTCG AGAGCTCAGA AATTTGGACT CAAGACAGTG CCGAGAGACC 
CACAAGATTG CAGTGTTTTA TGTTGCTGAG GGACAAGAAG ATAAACACTC CATTCTTACT 
AATACTGGGG GAAGTCAAGC CTATGAGGAT TTTGTAGCTG GCCTTGGCTG GGAGGTAAAT 
CTCACAAACC ATTGTGGCTT TATGGGAGGA CTGCAAAAAA ACAAAAGCAC TGGATTGACC 
ACTCCATATT TTGCTACCTC AACAGTAGAA GTAATGTTCC ATGTGTCAAC AAGAATGCCT 
TCTGATTCAG ATGACTCTCT AACAAAAAAG CTAAGACATT TAGGGAACGA TGAAGTACAC 
ATTGTTTGGT CAGAGCATAC TCGAGATTAT AGAAGAGGAA TAATTCCAAC CGAATTTGGT 
GATGTTCTTA TTGTTATCTA TCCCATGAAA AACCACATGT TCAGTATCCA GATCATGAAG 
AAGCCTGAGG TTCCATTTTT TGGTCCACTT TTTGATGGTG CTATTGTGAA TGGAAAAATT 
CTTCCTATTA TGGTTCGCGC AACAGCTATA AATGCAAGCC GTGCATTGAA ATCATTAATC 
CCTTTATACC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTTCAGCAT 
CACCTAGAAC CTACAACTTT TGAGGATTTT GCTGCTCAGG TTTTCTGTCC AGCACCTTAC 
CACCATTTGC CCTCAGATGC AGAAAGAAAT GCTGTGATAT TTCTTCTCCT TCCAAAACCT 
CCACTAGAAA GCATTGGCCA CATCAAGGTA AAGGGCAAAG GCTTGAAACG GAAGGTCTTT 
CACGCTAGCT GTGAATGGCT CCTGCCCAGA GACTCAGCCG GGAGAGCCTC CAGCAGCAGC 
AGCAGCAGCA GCGCAGGCGG ATGGAACCGA CTTAGCCTCA CCCATGTCTC CTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142864.1
CDS	1..6660
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287378.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287378.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AGTACACTTA CAGCGACTAA GCAGAATACA 
GGACATGCAA TAGGAATGTC TTCTCCAGAA GTTCAGTCGC AGTTTTGTGA CAGAAATGAG 
AATGTCCATA ATAGCCAATT ACTTTCTGCT TTTACATCTT ATGTAAATTG TGCAACAGTG 
TGTTGGAAGC ATAGGAGATG TACATACAAG TTTTACATGG AGGATTTTGG AGATTTCAGC 
TTTGGCACAG AGGCAGGGAC GCCAGCTTCC ACTGATGCTG ATGCAATTTC AGGACAGCAG 
CAGAGTGCTG AGGAACAAGA AGTAGCCAGT CTAACTACAC TCCACATAGA TTCGGAAACA 
AGTAGTCTGA ATCAGCAACC GTTGTCTGCT GAAGCTGCAA CTGTTACTGG CTCTGAAAGT 
GCTTCTCCAA TTCAATCTGC TCTTGGATCC CGATCACAGA CACCCTCTCC TGCCACTCTT 
CATGCAGATC ATATTGAGCA GAAGGATCTG CAACTGGATG AGAAGCTCCA CCACTCTGTT 
TTGCAGACGC CAGATGACCT AGAAACTGGT GAATTTCCCT CAGAATGTTG CAGTGTTATG 
GCTGGGGGAA CACTTACAGG ATGGCACGCA GATGTTGCTA CTGTGATGTG GAGACGAATG 
CTAGGAATTT TAGGTGATGT CAACAGCATC ATGGATCCAG AGATTCATGC CCAGGTTTTT 
GATTACCTGT GTGAACTGTG GCAGAATCTG GCCAAGATAA GAGACAATCT TGGTATTTCA 
ACAGATAACC TCACTTCACC ATCTCCACCA GTTTTAATCC CACCACTGAG AATTCTAACA 
CCTTGGCTGT TCAAGGCAAC AATGCTGACT GATAAATACA AACAAGGGAA GCTACATGCT 
TATAAACTCA TTTGTAAGAC AATGAAGCGA AGACAAGATG TATCTCCAAA CAAGGATTTT 
TTGACTCATT TCTATAATAT AATGCACTGT GGACTTCTTC ATGTTGATCA GGATATTGTC 
AATACAATCA TCAAGCACTG CTCTCCTCAG TTCTTTTCAC TTAGTTTGCC TGGTGCCACC 
ATGCTTATTA TGGATTTCAT TGTAGCAGCA GGAAGGGTGG CAGCTTCTTC TTTTCTCAAT 
GCACCAAGAG TTGAAGCTCA AGTTCTTTTA GGATCTCTTG TCTGTTTTCC CAATTTATAT 
CATGAACTAC CAGCTCTTCA CCCAAACATT CCTGACATAG CTGTGTCTCA GTTTACAGAT 
GTTAAGGAGC TCATAATTAA AACTGTGTTA AGTTCAGCCA GAGAGGAGCC ATCTGGTCCT 
GCAAGATGTG TTGCTCTTTG CAGCCTTGGT ATTTGGATCT GTGAGGAGCT AGTTCATGAA 
TCTCATCATC CTCAGATCAA GGAAGCATTG AATGTGATTT GTGTTTCTTT AAAGTTTCCG 
AATAAGACAG TAGCTCAAGT TGCCTGCAGT ATGCTGAATA TGCTGATACA TTATGTGCAT 
AGACTTCAAG TGTATCAAGC TGATTCACCT TTAAGAATTA TTCAAATTCT GATAGCTACA 
ATTACCCATC TGCTACCCGG TACAGAAGCT TCATCCTATG AACAGGACAA GAGGTTGGTA 
GTTTCCTTAC TTCTGTGCTT ATTGGATTGG ATAATGGCCT TGCCATTGAA GACCTTGCTG 
CAGCCTCTTC ACGCTACAGG AGCAGAAAAT GATAAGACTG AGAGATCTGT TCTTAATTGT 
ATATATAAGG TTCTACATGG ATGTGTCTAT GGATCTCAGT GTTTTAGTAA CCCCAAATAT 
TTTCCCCTAA GTCTTTCTGA TTTGGCATGT GTGGATTACG ATCCATTTAT GCATTTAGAA 
AGCTTGAAGG AGCCAGAACC ACTGCATTCT CCAGACTCAG AGCGTTCTTC TAAACTTCAA 
CCAGTCACCG AAGTGAAAAC TCACATGCAA CAAGGATTAA TTTCTGTTGC TGCTCGTACT 
GTGATAACGC ATCTAGTCAA CCACTTAGGT CACTATCCTA TGAGTGGAGG ACCTGCTATG 
CTGACTAGTC AAGTGTGTGA AAATAATGAC AATCCATATA GTGAAAGTCC TGAACTTTCT 
CCTGAACTTT TTGAGAACCC AAACCTTCAA TTCTTTGTGT TAAACAATAC TTCCTTGGTG 
TCTTGCATAC AAATCCAAGC AGAAGATGAC ATGCCTGGGG GAGGACTGTC TGCTGGACTT 
GCATCTGCTA ATTCAAATGT CAGAATTATA GTGCGTGATC TGTCTGGCAA ATACTCATGG 
GATTCAGCCA TTCTGTATGG ACCATCATCC TTATGTGCAT CTTCACAACA AACATCTTTT 
GCCCTTTCAT TATCTCAACA AGAAAAAACG GAAGATCCCC TTTCTTCTTT TGAGCATGTG 
GAGGACATAT CTGCAAGGGA TGGAATTACA CTCCAGGTAA AAAGGAAATT CAGAGACACT 
GTGCCAACAT GGGATACAAT TAGGGATGAA GAAGATGCCC TTGATGAGCT CTTGCAGTAT 
TTAGGTGTTA CAAGCCCTGA ATGCTTACAG AGAACTGGAG TCTCACTCAA CATCCCTGCT 
CCTCAACCAG TCTGCATCTC AGAGAAGCAG GAGAATGACG TCATCAATGC AATTCTGAAA 
CAGCATACAG AAGAGAGAGA GTTTGTTGAA AAACATTTCA ATGATTTAAA TATGAGGGCT 
ATGGAGCAGG ATGAGCCAAC ATCGCAAAAG CCCCAGTCAG CATTTTACTA CTGCAGACTA 
CTTCTCAGTA TACTGGGAAT GAATTCTTGG GATAAAAGAA GGAGCTTTCA TCTCCTGAAG 
AAAAATGAAA AGTTACTTCG AGAGCTCAGA AATTTGGACT CAAGACAGTG CCGAGAGACC 
CACAAGATTG CAGTGTTTTA TGTTGCTGAG GGACAAGAAG ATAAACACTC CATTCTTACT 
AATACTGGGG GAAGTCAAGC CTATGAGGAT TTTGTAGCTG GCCTTGGCTG GGAGGTAAAT 
CTCACAAACC ATTGTGGCTT TATGGGAGGA CTGCAAAAAA ACAAAAGCAC TGGATTGACC 
ACTCCATATT TTGCTACCTC AACAGTAGAA GTAATGTTCC ATGTGTCAAC AAGAATGCCT 
TCTGATTCAG ATGACTCTCT AACAAAAAAG CTAAGACATT TAGGGAACGA TGAAGTACAC 
ATTGTTTGGT CAGAGCATAC TCGAGATTAT AGAAGAGGAA TAATTCCAAC CGAATTTGGT 
GATGTTCTTA TTGTTATCTA TCCCATGAAA AACCACATGT TCAGTATCCA GATCATGAAG 
AAGCCTGAGG TTCCATTTTT TGGTCCACTT TTTGATGGTG CTATTGTGAA TGGAAAAATT 
CTTCCTATTA TGGTTCGCGC AACAGCTATA AATGCAAGCC GTGCATTGAA ATCATTAATC 
CCTTTATACC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTTCAGCAT 
CACCTAGAAC CTACAACTTT TGAGGATTTT GCTGCTCAGG TTTTCTGTCC AGCACCTTAC 
CACCATTTGC CCTCAGATGC AGAAAGAAAT GCTGTGATAT TTCTTCTCCT TCCAAAACCT 
CCACTAGAAA GCATTGGCCA CATCAAGGTA AAGGGCAAAG GCTTGAAACG GAAGGTCTTT 
CACGCTAGCT GTGAATGGCT CCTGCCCAGA GACTCAGCCG GGAGAGCCTC CAGCAGCAGC 
AGCAGCAGCA GCGCAGGCGG ATGGAACCGA CTTAGCCTCA CCCATGTCTC CTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287378.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142864.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X12, mRNA.

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CloneID	OGa36823
Clone ID Related Accession (Same CDS sequence)	XM_015287378.1 , XM_015287378.2
Accession Version	XM_015287378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AGTACACTTA CAGCGACTAA GCAGAATACA 
GGACATGCAA TAGGAATGTC TTCTCCAGAA GTTCAGTCGC AGTTTTGTGA CAGAAATGAG 
AATGTCCATA ATAGCCAATT ACTTTCTGCT TTTACATCTT ATGTAAATTG TGCAACAGTG 
TGTTGGAAGC ATAGGAGATG TACATACAAG TTTTACATGG AGGATTTTGG AGATTTCAGC 
TTTGGCACAG AGGCAGGGAC GCCAGCTTCC ACTGATGCTG ATGCAATTTC AGGACAGCAG 
CAGAGTGCTG AGGAACAAGA AGTAGCCAGT CTAACTACAC TCCACATAGA TTCGGAAACA 
AGTAGTCTGA ATCAGCAACC GTTGTCTGCT GAAGCTGCAA CTGTTACTGG CTCTGAAAGT 
GCTTCTCCAA TTCAATCTGC TCTTGGATCC CGATCACAGA CACCCTCTCC TGCCACTCTT 
CATGCAGATC ATATTGAGCA GAAGGATCTG CAACTGGATG AGAAGCTCCA CCACTCTGTT 
TTGCAGACGC CAGATGACCT AGAAACTGGT GAATTTCCCT CAGAATGTTG CAGTGTTATG 
GCTGGGGGAA CACTTACAGG ATGGCACGCA GATGTTGCTA CTGTGATGTG GAGACGAATG 
CTAGGAATTT TAGGTGATGT CAACAGCATC ATGGATCCAG AGATTCATGC CCAGGTTTTT 
GATTACCTGT GTGAACTGTG GCAGAATCTG GCCAAGATAA GAGACAATCT TGGTATTTCA 
ACAGATAACC TCACTTCACC ATCTCCACCA GTTTTAATCC CACCACTGAG AATTCTAACA 
CCTTGGCTGT TCAAGGCAAC AATGCTGACT GATAAATACA AACAAGGGAA GCTACATGCT 
TATAAACTCA TTTGTAAGAC AATGAAGCGA AGACAAGATG TATCTCCAAA CAAGGATTTT 
TTGACTCATT TCTATAATAT AATGCACTGT GGACTTCTTC ATGTTGATCA GGATATTGTC 
AATACAATCA TCAAGCACTG CTCTCCTCAG TTCTTTTCAC TTAGTTTGCC TGGTGCCACC 
ATGCTTATTA TGGATTTCAT TGTAGCAGCA GGAAGGGTGG CAGCTTCTTC TTTTCTCAAT 
GCACCAAGAG TTGAAGCTCA AGTTCTTTTA GGATCTCTTG TCTGTTTTCC CAATTTATAT 
CATGAACTAC CAGCTCTTCA CCCAAACATT CCTGACATAG CTGTGTCTCA GTTTACAGAT 
GTTAAGGAGC TCATAATTAA AACTGTGTTA AGTTCAGCCA GAGAGGAGCC ATCTGGTCCT 
GCAAGATGTG TTGCTCTTTG CAGCCTTGGT ATTTGGATCT GTGAGGAGCT AGTTCATGAA 
TCTCATCATC CTCAGATCAA GGAAGCATTG AATGTGATTT GTGTTTCTTT AAAGTTTCCG 
AATAAGACAG TAGCTCAAGT TGCCTGCAGT ATGCTGAATA TGCTGATACA TTATGTGCAT 
AGACTTCAAG TGTATCAAGC TGATTCACCT TTAAGAATTA TTCAAATTCT GATAGCTACA 
ATTACCCATC TGCTACCCGG TACAGAAGCT TCATCCTATG AACAGGACAA GAGGTTGGTA 
GTTTCCTTAC TTCTGTGCTT ATTGGATTGG ATAATGGCCT TGCCATTGAA GACCTTGCTG 
CAGCCTCTTC ACGCTACAGG AGCAGAAAAT GATAAGACTG AGAGATCTGT TCTTAATTGT 
ATATATAAGG TTCTACATGG ATGTGTCTAT GGATCTCAGT GTTTTAGTAA CCCCAAATAT 
TTTCCCCTAA GTCTTTCTGA TTTGGCATGT GTGGATTACG ATCCATTTAT GCATTTAGAA 
AGCTTGAAGG AGCCAGAACC ACTGCATTCT CCAGACTCAG AGCGTTCTTC TAAACTTCAA 
CCAGTCACCG AAGTGAAAAC TCACATGCAA CAAGGATTAA TTTCTGTTGC TGCTCGTACT 
GTGATAACGC ATCTAGTCAA CCACTTAGGT CACTATCCTA TGAGTGGAGG ACCTGCTATG 
CTGACTAGTC AAGTGTGTGA AAATAATGAC AATCCATATA GTGAAAGTCC TGAACTTTCT 
CCTGAACTTT TTGAGAACCC AAACCTTCAA TTCTTTGTGT TAAACAATAC TTCCTTGGTG 
TCTTGCATAC AAATCCAAGC AGAAGATGAC ATGCCTGGGG GAGGACTGTC TGCTGGACTT 
GCATCTGCTA ATTCAAATGT CAGAATTATA GTGCGTGATC TGTCTGGCAA ATACTCATGG 
GATTCAGCCA TTCTGTATGG ACCATCATCC TTATGTGCAT CTTCACAACA AACATCTTTT 
GCCCTTTCAT TATCTCAACA AGAAAAAACG GAAGATCCCC TTTCTTCTTT TGAGCATGTG 
GAGGACATAT CTGCAAGGGA TGGAATTACA CTCCAGGTAA AAAGGAAATT CAGAGACACT 
GTGCCAACAT GGGATACAAT TAGGGATGAA GAAGATGCCC TTGATGAGCT CTTGCAGTAT 
TTAGGTGTTA CAAGCCCTGA ATGCTTACAG AGAACTGGAG TCTCACTCAA CATCCCTGCT 
CCTCAACCAG TCTGCATCTC AGAGAAGCAG GAGAATGACG TCATCAATGC AATTCTGAAA 
CAGCATACAG AAGAGAGAGA GTTTGTTGAA AAACATTTCA ATGATTTAAA TATGAGGGCT 
ATGGAGCAGG ATGAGCCAAC ATCGCAAAAG CCCCAGTCAG CATTTTACTA CTGCAGACTA 
CTTCTCAGTA TACTGGGAAT GAATTCTTGG GATAAAAGAA GGAGCTTTCA TCTCCTGAAG 
AAAAATGAAA AGTTACTTCG AGAGCTCAGA AATTTGGACT CAAGACAGTG CCGAGAGACC 
CACAAGATTG CAGTGTTTTA TGTTGCTGAG GGACAAGAAG ATAAACACTC CATTCTTACT 
AATACTGGGG GAAGTCAAGC CTATGAGGAT TTTGTAGCTG GCCTTGGCTG GGAGGTAAAT 
CTCACAAACC ATTGTGGCTT TATGGGAGGA CTGCAAAAAA ACAAAAGCAC TGGATTGACC 
ACTCCATATT TTGCTACCTC AACAGTAGAA GTAATGTTCC ATGTGTCAAC AAGAATGCCT 
TCTGATTCAG ATGACTCTCT AACAAAAAAG CTAAGACATT TAGGGAACGA TGAAGTACAC 
ATTGTTTGGT CAGAGCATAC TCGAGATTAT AGAAGAGGAA TAATTCCAAC CGAATTTGGT 
GATGTTCTTA TTGTTATCTA TCCCATGAAA AACCACATGT TCAGTATCCA GATCATGAAG 
AAGCCTGAGG TTCCATTTTT TGGTCCACTT TTTGATGGTG CTATTGTGAA TGGAAAAATT 
CTTCCTATTA TGGTTCGCGC AACAGCTATA AATGCAAGCC GTGCATTGAA ATCATTAATC 
CCTTTATACC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTTCAGCAT 
CACCTAGAAC CTACAACTTT TGAGGATTTT GCTGCTCAGG TTTTCTGTCC AGCACCTTAC 
CACCATTTGC CCTCAGATGC AGAAAGAAAT GCTGTGATAT TTCTTCTCCT TCCAAAACCT 
CCACTAGAAA GCATTGGCCA CATCAAGGTA AAGGGCAAAG GCTTGAAACG GAAGGTCTTT 
CACGCTAGCT GTGAATGGCT CCTGCCCAGA GACTCAGCCG GGAGAGCCTC CAGCAGCAGC 
AGCAGCAGCA GCGCAGGCGG ATGGAACCGA CTTAGCCTCA CCCATGTCTC CTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142864.1
CDS	266..6925
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287378.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287378.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AGTACACTTA CAGCGACTAA GCAGAATACA 
GGACATGCAA TAGGAATGTC TTCTCCAGAA GTTCAGTCGC AGTTTTGTGA CAGAAATGAG 
AATGTCCATA ATAGCCAATT ACTTTCTGCT TTTACATCTT ATGTAAATTG TGCAACAGTG 
TGTTGGAAGC ATAGGAGATG TACATACAAG TTTTACATGG AGGATTTTGG AGATTTCAGC 
TTTGGCACAG AGGCAGGGAC GCCAGCTTCC ACTGATGCTG ATGCAATTTC AGGACAGCAG 
CAGAGTGCTG AGGAACAAGA AGTAGCCAGT CTAACTACAC TCCACATAGA TTCGGAAACA 
AGTAGTCTGA ATCAGCAACC GTTGTCTGCT GAAGCTGCAA CTGTTACTGG CTCTGAAAGT 
GCTTCTCCAA TTCAATCTGC TCTTGGATCC CGATCACAGA CACCCTCTCC TGCCACTCTT 
CATGCAGATC ATATTGAGCA GAAGGATCTG CAACTGGATG AGAAGCTCCA CCACTCTGTT 
TTGCAGACGC CAGATGACCT AGAAACTGGT GAATTTCCCT CAGAATGTTG CAGTGTTATG 
GCTGGGGGAA CACTTACAGG ATGGCACGCA GATGTTGCTA CTGTGATGTG GAGACGAATG 
CTAGGAATTT TAGGTGATGT CAACAGCATC ATGGATCCAG AGATTCATGC CCAGGTTTTT 
GATTACCTGT GTGAACTGTG GCAGAATCTG GCCAAGATAA GAGACAATCT TGGTATTTCA 
ACAGATAACC TCACTTCACC ATCTCCACCA GTTTTAATCC CACCACTGAG AATTCTAACA 
CCTTGGCTGT TCAAGGCAAC AATGCTGACT GATAAATACA AACAAGGGAA GCTACATGCT 
TATAAACTCA TTTGTAAGAC AATGAAGCGA AGACAAGATG TATCTCCAAA CAAGGATTTT 
TTGACTCATT TCTATAATAT AATGCACTGT GGACTTCTTC ATGTTGATCA GGATATTGTC 
AATACAATCA TCAAGCACTG CTCTCCTCAG TTCTTTTCAC TTAGTTTGCC TGGTGCCACC 
ATGCTTATTA TGGATTTCAT TGTAGCAGCA GGAAGGGTGG CAGCTTCTTC TTTTCTCAAT 
GCACCAAGAG TTGAAGCTCA AGTTCTTTTA GGATCTCTTG TCTGTTTTCC CAATTTATAT 
CATGAACTAC CAGCTCTTCA CCCAAACATT CCTGACATAG CTGTGTCTCA GTTTACAGAT 
GTTAAGGAGC TCATAATTAA AACTGTGTTA AGTTCAGCCA GAGAGGAGCC ATCTGGTCCT 
GCAAGATGTG TTGCTCTTTG CAGCCTTGGT ATTTGGATCT GTGAGGAGCT AGTTCATGAA 
TCTCATCATC CTCAGATCAA GGAAGCATTG AATGTGATTT GTGTTTCTTT AAAGTTTCCG 
AATAAGACAG TAGCTCAAGT TGCCTGCAGT ATGCTGAATA TGCTGATACA TTATGTGCAT 
AGACTTCAAG TGTATCAAGC TGATTCACCT TTAAGAATTA TTCAAATTCT GATAGCTACA 
ATTACCCATC TGCTACCCGG TACAGAAGCT TCATCCTATG AACAGGACAA GAGGTTGGTA 
GTTTCCTTAC TTCTGTGCTT ATTGGATTGG ATAATGGCCT TGCCATTGAA GACCTTGCTG 
CAGCCTCTTC ACGCTACAGG AGCAGAAAAT GATAAGACTG AGAGATCTGT TCTTAATTGT 
ATATATAAGG TTCTACATGG ATGTGTCTAT GGATCTCAGT GTTTTAGTAA CCCCAAATAT 
TTTCCCCTAA GTCTTTCTGA TTTGGCATGT GTGGATTACG ATCCATTTAT GCATTTAGAA 
AGCTTGAAGG AGCCAGAACC ACTGCATTCT CCAGACTCAG AGCGTTCTTC TAAACTTCAA 
CCAGTCACCG AAGTGAAAAC TCACATGCAA CAAGGATTAA TTTCTGTTGC TGCTCGTACT 
GTGATAACGC ATCTAGTCAA CCACTTAGGT CACTATCCTA TGAGTGGAGG ACCTGCTATG 
CTGACTAGTC AAGTGTGTGA AAATAATGAC AATCCATATA GTGAAAGTCC TGAACTTTCT 
CCTGAACTTT TTGAGAACCC AAACCTTCAA TTCTTTGTGT TAAACAATAC TTCCTTGGTG 
TCTTGCATAC AAATCCAAGC AGAAGATGAC ATGCCTGGGG GAGGACTGTC TGCTGGACTT 
GCATCTGCTA ATTCAAATGT CAGAATTATA GTGCGTGATC TGTCTGGCAA ATACTCATGG 
GATTCAGCCA TTCTGTATGG ACCATCATCC TTATGTGCAT CTTCACAACA AACATCTTTT 
GCCCTTTCAT TATCTCAACA AGAAAAAACG GAAGATCCCC TTTCTTCTTT TGAGCATGTG 
GAGGACATAT CTGCAAGGGA TGGAATTACA CTCCAGGTAA AAAGGAAATT CAGAGACACT 
GTGCCAACAT GGGATACAAT TAGGGATGAA GAAGATGCCC TTGATGAGCT CTTGCAGTAT 
TTAGGTGTTA CAAGCCCTGA ATGCTTACAG AGAACTGGAG TCTCACTCAA CATCCCTGCT 
CCTCAACCAG TCTGCATCTC AGAGAAGCAG GAGAATGACG TCATCAATGC AATTCTGAAA 
CAGCATACAG AAGAGAGAGA GTTTGTTGAA AAACATTTCA ATGATTTAAA TATGAGGGCT 
ATGGAGCAGG ATGAGCCAAC ATCGCAAAAG CCCCAGTCAG CATTTTACTA CTGCAGACTA 
CTTCTCAGTA TACTGGGAAT GAATTCTTGG GATAAAAGAA GGAGCTTTCA TCTCCTGAAG 
AAAAATGAAA AGTTACTTCG AGAGCTCAGA AATTTGGACT CAAGACAGTG CCGAGAGACC 
CACAAGATTG CAGTGTTTTA TGTTGCTGAG GGACAAGAAG ATAAACACTC CATTCTTACT 
AATACTGGGG GAAGTCAAGC CTATGAGGAT TTTGTAGCTG GCCTTGGCTG GGAGGTAAAT 
CTCACAAACC ATTGTGGCTT TATGGGAGGA CTGCAAAAAA ACAAAAGCAC TGGATTGACC 
ACTCCATATT TTGCTACCTC AACAGTAGAA GTAATGTTCC ATGTGTCAAC AAGAATGCCT 
TCTGATTCAG ATGACTCTCT AACAAAAAAG CTAAGACATT TAGGGAACGA TGAAGTACAC 
ATTGTTTGGT CAGAGCATAC TCGAGATTAT AGAAGAGGAA TAATTCCAAC CGAATTTGGT 
GATGTTCTTA TTGTTATCTA TCCCATGAAA AACCACATGT TCAGTATCCA GATCATGAAG 
AAGCCTGAGG TTCCATTTTT TGGTCCACTT TTTGATGGTG CTATTGTGAA TGGAAAAATT 
CTTCCTATTA TGGTTCGCGC AACAGCTATA AATGCAAGCC GTGCATTGAA ATCATTAATC 
CCTTTATACC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTTCAGCAT 
CACCTAGAAC CTACAACTTT TGAGGATTTT GCTGCTCAGG TTTTCTGTCC AGCACCTTAC 
CACCATTTGC CCTCAGATGC AGAAAGAAAT GCTGTGATAT TTCTTCTCCT TCCAAAACCT 
CCACTAGAAA GCATTGGCCA CATCAAGGTA AAGGGCAAAG GCTTGAAACG GAAGGTCTTT 
CACGCTAGCT GTGAATGGCT CCTGCCCAGA GACTCAGCCG GGAGAGCCTC CAGCAGCAGC 
AGCAGCAGCA GCGCAGGCGG ATGGAACCGA CTTAGCCTCA CCCATGTCTC CTCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287379.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142865.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X10, mRNA.

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CloneID	OGa36824
Clone ID Related Accession (Same CDS sequence)	XM_015287379.1 , XM_015287379.2
Accession Version	XM_015287379.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA GTACACTTAC AGCGACTAAG CAGAATACAG GACATGCAAT AGGAATGTCT 
TCTCCAGAAG TTCAGTCGCA GTTTTGTGAC AGAAATGAGA ATGTCCATAA TAGCCAATTA 
CTTTCTGCTT TTACATCTTA TGTAAATTGT GCAACAGTGT GTTGGAAGCA TAGGAGATGT 
ACATACAAGT TTTACATGGA GGATTTTGGA GATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GAAAGAAATG CTGTGATATT TCTTCTCCTT CCAAAACCTC CACTAGAAAG CATTGGCCAC 
ATCAAGGTAA AGGGCAAAGG CTTGAAACGG AAGGTCTTTC ACGCTAGCTG TGAATGGCTC 
CTGCCCAGAG ACTCAGCCGG GAGAGCCTCC AGCAGCAGCA GCAGCAGCAG CGCAGGCGGA 
TGGAACCGAC TTAGCCTCAC CCATGTCTCC TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142865.1
CDS	266..6784
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287379.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287379.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA GTACACTTAC AGCGACTAAG CAGAATACAG GACATGCAAT AGGAATGTCT 
TCTCCAGAAG TTCAGTCGCA GTTTTGTGAC AGAAATGAGA ATGTCCATAA TAGCCAATTA 
CTTTCTGCTT TTACATCTTA TGTAAATTGT GCAACAGTGT GTTGGAAGCA TAGGAGATGT 
ACATACAAGT TTTACATGGA GGATTTTGGA GATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GAAAGAAATG CTGTGATATT TCTTCTCCTT CCAAAACCTC CACTAGAAAG CATTGGCCAC 
ATCAAGGTAA AGGGCAAAGG CTTGAAACGG AAGGTCTTTC ACGCTAGCTG TGAATGGCTC 
CTGCCCAGAG ACTCAGCCGG GAGAGCCTCC AGCAGCAGCA GCAGCAGCAG CGCAGGCGGA 
TGGAACCGAC TTAGCCTCAC CCATGTCTCC TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287379.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142865.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X13, mRNA.

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CloneID	OGa36824
Clone ID Related Accession (Same CDS sequence)	XM_015287379.1 , XM_015287379.2
Accession Version	XM_015287379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA GTACACTTAC AGCGACTAAG CAGAATACAG GACATGCAAT AGGAATGTCT 
TCTCCAGAAG TTCAGTCGCA GTTTTGTGAC AGAAATGAGA ATGTCCATAA TAGCCAATTA 
CTTTCTGCTT TTACATCTTA TGTAAATTGT GCAACAGTGT GTTGGAAGCA TAGGAGATGT 
ACATACAAGT TTTACATGGA GGATTTTGGA GATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GAAAGAAATG CTGTGATATT TCTTCTCCTT CCAAAACCTC CACTAGAAAG CATTGGCCAC 
ATCAAGGTAA AGGGCAAAGG CTTGAAACGG AAGGTCTTTC ACGCTAGCTG TGAATGGCTC 
CTGCCCAGAG ACTCAGCCGG GAGAGCCTCC AGCAGCAGCA GCAGCAGCAG CGCAGGCGGA 
TGGAACCGAC TTAGCCTCAC CCATGTCTCC TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142865.1
CDS	265..6783
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287379.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287379.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA GTACACTTAC AGCGACTAAG CAGAATACAG GACATGCAAT AGGAATGTCT 
TCTCCAGAAG TTCAGTCGCA GTTTTGTGAC AGAAATGAGA ATGTCCATAA TAGCCAATTA 
CTTTCTGCTT TTACATCTTA TGTAAATTGT GCAACAGTGT GTTGGAAGCA TAGGAGATGT 
ACATACAAGT TTTACATGGA GGATTTTGGA GATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GAAAGAAATG CTGTGATATT TCTTCTCCTT CCAAAACCTC CACTAGAAAG CATTGGCCAC 
ATCAAGGTAA AGGGCAAAGG CTTGAAACGG AAGGTCTTTC ACGCTAGCTG TGAATGGCTC 
CTGCCCAGAG ACTCAGCCGG GAGAGCCTCC AGCAGCAGCA GCAGCAGCAG CGCAGGCGGA 
TGGAACCGAC TTAGCCTCAC CCATGTCTCC TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287380.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142866.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X11, mRNA.

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CloneID	OGa36825
Clone ID Related Accession (Same CDS sequence)	XM_015287380.1 , XM_015287380.2
Accession Version	XM_015287380.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GAAAGAAATG CTGTGATATT TCTTCTCCTT CCAAAACCTC CACTAGAAAG CATTGGCCAC 
ATCAAGGTAA AGGGCAAAGG CTTGAAACGG AAGGTCTTTC ACGCTAGCTG TGAATGGCTC 
CTGCCCAGAG ACTCAGCCGG GAGAGCCTCC AGCAGCAGCA GCAGCAGCAG CGCAGGCGGA 
TGGAACCGAC TTAGCCTCAC CCATGTCTCC TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142866.1
CDS	1..6459
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287380.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287380.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GAAAGAAATG CTGTGATATT TCTTCTCCTT CCAAAACCTC CACTAGAAAG CATTGGCCAC 
ATCAAGGTAA AGGGCAAAGG CTTGAAACGG AAGGTCTTTC ACGCTAGCTG TGAATGGCTC 
CTGCCCAGAG ACTCAGCCGG GAGAGCCTCC AGCAGCAGCA GCAGCAGCAG CGCAGGCGGA 
TGGAACCGAC TTAGCCTCAC CCATGTCTCC TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287380.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142866.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X14, mRNA.

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CloneID	OGa36825
Clone ID Related Accession (Same CDS sequence)	XM_015287380.1 , XM_015287380.2
Accession Version	XM_015287380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GAAAGAAATG CTGTGATATT TCTTCTCCTT CCAAAACCTC CACTAGAAAG CATTGGCCAC 
ATCAAGGTAA AGGGCAAAGG CTTGAAACGG AAGGTCTTTC ACGCTAGCTG TGAATGGCTC 
CTGCCCAGAG ACTCAGCCGG GAGAGCCTCC AGCAGCAGCA GCAGCAGCAG CGCAGGCGGA 
TGGAACCGAC TTAGCCTCAC CCATGTCTCC TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142866.1
CDS	1..6459
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287380.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287380.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GAAAGAAATG CTGTGATATT TCTTCTCCTT CCAAAACCTC CACTAGAAAG CATTGGCCAC 
ATCAAGGTAA AGGGCAAAGG CTTGAAACGG AAGGTCTTTC ACGCTAGCTG TGAATGGCTC 
CTGCCCAGAG ACTCAGCCGG GAGAGCCTCC AGCAGCAGCA GCAGCAGCAG CGCAGGCGGA 
TGGAACCGAC TTAGCCTCAC CCATGTCTCC TCGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287381.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142867.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X12, mRNA.

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CloneID	OGa36826
Clone ID Related Accession (Same CDS sequence)	XM_015287381.1 , XM_015287381.2
Accession Version	XM_015287381.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG AAAGAAATGC TGTGATATTT 
CTTCTCCTTC CAAAACCTCC ACTAGAAAGC ATTGGCCACA TCAAGGTAAA GGGCAAAGGC 
TTGAAACGGA AGGTCTTTCA CGCTAGCTGT GAATGGCTCC TGCCCAGAGA CTCAGCCGGG 
AGAGCCTCCA GCAGCAGCAG CAGCAGCAGC GCAGGCGGAT GGAACCGACT TAGCCTCACC 
CATGTCTCCT CGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142867.1
CDS	1..6318
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287381.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287381.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG AAAGAAATGC TGTGATATTT 
CTTCTCCTTC CAAAACCTCC ACTAGAAAGC ATTGGCCACA TCAAGGTAAA GGGCAAAGGC 
TTGAAACGGA AGGTCTTTCA CGCTAGCTGT GAATGGCTCC TGCCCAGAGA CTCAGCCGGG 
AGAGCCTCCA GCAGCAGCAG CAGCAGCAGC GCAGGCGGAT GGAACCGACT TAGCCTCACC 
CATGTCTCCT CGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287381.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142867.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X15, mRNA.

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CloneID	OGa36826
Clone ID Related Accession (Same CDS sequence)	XM_015287381.1 , XM_015287381.2
Accession Version	XM_015287381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG AAAGAAATGC TGTGATATTT 
CTTCTCCTTC CAAAACCTCC ACTAGAAAGC ATTGGCCACA TCAAGGTAAA GGGCAAAGGC 
TTGAAACGGA AGGTCTTTCA CGCTAGCTGT GAATGGCTCC TGCCCAGAGA CTCAGCCGGG 
AGAGCCTCCA GCAGCAGCAG CAGCAGCAGC GCAGGCGGAT GGAACCGACT TAGCCTCACC 
CATGTCTCCT CGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142867.1
CDS	1..6318
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287381.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287381.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG AAAGAAATGC TGTGATATTT 
CTTCTCCTTC CAAAACCTCC ACTAGAAAGC ATTGGCCACA TCAAGGTAAA GGGCAAAGGC 
TTGAAACGGA AGGTCTTTCA CGCTAGCTGT GAATGGCTCC TGCCCAGAGA CTCAGCCGGG 
AGAGCCTCCA GCAGCAGCAG CAGCAGCAGC GCAGGCGGAT GGAACCGACT TAGCCTCACC 
CATGTCTCCT CGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287383.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142869.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X13, mRNA.

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CloneID	OGa36827
Clone ID Related Accession (Same CDS sequence)	XM_015287383.1 , XM_015287383.2
Accession Version	XM_015287383.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGAAA CTGGTGAATT TCCCTCAGAA TGTTGCAGTG TTATGGCTGG GGGAACACTT 
ACAGGATGGC ACGCAGATGT TGCTACTGTG ATGTGGAGAC GAATGCTAGG AATTTTAGGT 
GATGTCAACA GCATCATGGA TCCAGAGATT CATGCCCAGG TTTTTGATTA CCTGTGTGAA 
CTGTGGCAGA ATCTGGCCAA GATAAGAGAC AATCTTGGTA TTTCAACAGA TAACCTCACT 
TCACCATCTC CACCAGTTTT AATCCCACCA CTGAGAATTC TAACACCTTG GCTGTTCAAG 
GCAACAATGC TGACTGATAA ATACAAACAA GGGAAGCTAC ATGCTTATAA ACTCATTTGT 
AAGACAATGA AGCGAAGACA AGATGTATCT CCAAACAAGG ATTTTTTGAC TCATTTCTAT 
AATATAATGC ACTGTGGACT TCTTCATGTT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCTC CTCAGTTCTT TTCACTTAGT TTGCCTGGTG CCACCATGCT TATTATGGAT 
TTCATTGTAG CAGCAGGAAG GGTGGCAGCT TCTTCTTTTC TCAATGCACC AAGAGTTGAA 
GCTCAAGTTC TTTTAGGATC TCTTGTCTGT TTTCCCAATT TATATCATGA ACTACCAGCT 
CTTCACCCAA ACATTCCTGA CATAGCTGTG TCTCAGTTTA CAGATGTTAA GGAGCTCATA 
ATTAAAACTG TGTTAAGTTC AGCCAGAGAG GAGCCATCTG GTCCTGCAAG ATGTGTTGCT 
CTTTGCAGCC TTGGTATTTG GATCTGTGAG GAGCTAGTTC ATGAATCTCA TCATCCTCAG 
ATCAAGGAAG CATTGAATGT GATTTGTGTT TCTTTAAAGT TTCCGAATAA GACAGTAGCT 
CAAGTTGCCT GCAGTATGCT GAATATGCTG ATACATTATG TGCATAGACT TCAAGTGTAT 
CAAGCTGATT CACCTTTAAG AATTATTCAA ATTCTGATAG CTACAATTAC CCATCTGCTA 
CCCGGTACAG AAGCTTCATC CTATGAACAG GACAAGAGGT TGGTAGTTTC CTTACTTCTG 
TGCTTATTGG ATTGGATAAT GGCCTTGCCA TTGAAGACCT TGCTGCAGCC TCTTCACGCT 
ACAGGAGCAG AAAATGATAA GACTGAGAGA TCTGTTCTTA ATTGTATATA TAAGGTTCTA 
CATGGATGTG TCTATGGATC TCAGTGTTTT AGTAACCCCA AATATTTTCC CCTAAGTCTT 
TCTGATTTGG CATGTGTGGA TTACGATCCA TTTATGCATT TAGAAAGCTT GAAGGAGCCA 
GAACCACTGC ATTCTCCAGA CTCAGAGCGT TCTTCTAAAC TTCAACCAGT CACCGAAGTG 
AAAACTCACA TGCAACAAGG ATTAATTTCT GTTGCTGCTC GTACTGTGAT AACGCATCTA 
GTCAACCACT TAGGTCACTA TCCTATGAGT GGAGGACCTG CTATGCTGAC TAGTCAAGTG 
TGTGAAAATA ATGACAATCC ATATAGTGAA AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG 
AACCCAAACC TTCAATTCTT TGTGTTAAAC AATACTTCCT TGGTGTCTTG CATACAAATC 
CAAGCAGAAG ATGACATGCC TGGGGGAGGA CTGTCTGCTG GACTTGCATC TGCTAATTCA 
AATGTCAGAA TTATAGTGCG TGATCTGTCT GGCAAATACT CATGGGATTC AGCCATTCTG 
TATGGACCAT CATCCTTATG TGCATCTTCA CAACAAACAT CTTTTGCCCT TTCATTATCT 
CAACAAGAAA AAACGGAAGA TCCCCTTTCT TCTTTTGAGC ATGTGGAGGA CATATCTGCA 
AGGGATGGAA TTACACTCCA GGTAAAAAGG AAATTCAGAG ACACTGTGCC AACATGGGAT 
ACAATTAGGG ATGAAGAAGA TGCCCTTGAT GAGCTCTTGC AGTATTTAGG TGTTACAAGC 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTCAACATCC CTGCTCCTCA ACCAGTCTGC 
ATCTCAGAGA AGCAGGAGAA TGACGTCATC AATGCAATTC TGAAACAGCA TACAGAAGAG 
AGAGAGTTTG TTGAAAAACA TTTCAATGAT TTAAATATGA GGGCTATGGA GCAGGATGAG 
CCAACATCGC AAAAGCCCCA GTCAGCATTT TACTACTGCA GACTACTTCT CAGTATACTG 
GGAATGAATT CTTGGGATAA AAGAAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAGTTA 
CTTCGAGAGC TCAGAAATTT GGACTCAAGA CAGTGCCGAG AGACCCACAA GATTGCAGTG 
TTTTATGTTG CTGAGGGACA AGAAGATAAA CACTCCATTC TTACTAATAC TGGGGGAAGT 
CAAGCCTATG AGGATTTTGT AGCTGGCCTT GGCTGGGAGG TAAATCTCAC AAACCATTGT 
GGCTTTATGG GAGGACTGCA AAAAAACAAA AGCACTGGAT TGACCACTCC ATATTTTGCT 
ACCTCAACAG TAGAAGTAAT GTTCCATGTG TCAACAAGAA TGCCTTCTGA TTCAGATGAC 
TCTCTAACAA AAAAGCTAAG ACATTTAGGG AACGATGAAG TACACATTGT TTGGTCAGAG 
CATACTCGAG ATTATAGAAG AGGAATAATT CCAACCGAAT TTGGTGATGT TCTTATTGTT 
ATCTATCCCA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAGAAGCC TGAGGTTCCA 
TTTTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAATTCTTCC TATTATGGTT 
CGCGCAACAG CTATAAATGC AAGCCGTGCA TTGAAATCAT TAATCCCTTT ATACCAAAAC 
TTCTATGAGG AGAGAGCACG ATACCTGCAA ACAATTGTTC AGCATCACCT AGAACCTACA 
ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC TGTCCAGCAC CTTACCACCA TTTGCCCTCA 
GATGCAGAAA GAAATGCTGT GATATTTCTT CTCCTTCCAA AACCTCCACT AGAAAGCATT 
GGCCACATCA AGGTAAAGGG CAAAGGCTTG AAACGGAAGG TCTTTCACGC TAGCTGTGAA 
TGGCTCCTGC CCAGAGACTC AGCCGGGAGA GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA 
GGCGGATGGA ACCGACTTAG CCTCACCCAT GTCTCCTCGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142869.1
CDS	1..6165
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287383.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287383.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGAAA CTGGTGAATT TCCCTCAGAA TGTTGCAGTG TTATGGCTGG GGGAACACTT 
ACAGGATGGC ACGCAGATGT TGCTACTGTG ATGTGGAGAC GAATGCTAGG AATTTTAGGT 
GATGTCAACA GCATCATGGA TCCAGAGATT CATGCCCAGG TTTTTGATTA CCTGTGTGAA 
CTGTGGCAGA ATCTGGCCAA GATAAGAGAC AATCTTGGTA TTTCAACAGA TAACCTCACT 
TCACCATCTC CACCAGTTTT AATCCCACCA CTGAGAATTC TAACACCTTG GCTGTTCAAG 
GCAACAATGC TGACTGATAA ATACAAACAA GGGAAGCTAC ATGCTTATAA ACTCATTTGT 
AAGACAATGA AGCGAAGACA AGATGTATCT CCAAACAAGG ATTTTTTGAC TCATTTCTAT 
AATATAATGC ACTGTGGACT TCTTCATGTT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCTC CTCAGTTCTT TTCACTTAGT TTGCCTGGTG CCACCATGCT TATTATGGAT 
TTCATTGTAG CAGCAGGAAG GGTGGCAGCT TCTTCTTTTC TCAATGCACC AAGAGTTGAA 
GCTCAAGTTC TTTTAGGATC TCTTGTCTGT TTTCCCAATT TATATCATGA ACTACCAGCT 
CTTCACCCAA ACATTCCTGA CATAGCTGTG TCTCAGTTTA CAGATGTTAA GGAGCTCATA 
ATTAAAACTG TGTTAAGTTC AGCCAGAGAG GAGCCATCTG GTCCTGCAAG ATGTGTTGCT 
CTTTGCAGCC TTGGTATTTG GATCTGTGAG GAGCTAGTTC ATGAATCTCA TCATCCTCAG 
ATCAAGGAAG CATTGAATGT GATTTGTGTT TCTTTAAAGT TTCCGAATAA GACAGTAGCT 
CAAGTTGCCT GCAGTATGCT GAATATGCTG ATACATTATG TGCATAGACT TCAAGTGTAT 
CAAGCTGATT CACCTTTAAG AATTATTCAA ATTCTGATAG CTACAATTAC CCATCTGCTA 
CCCGGTACAG AAGCTTCATC CTATGAACAG GACAAGAGGT TGGTAGTTTC CTTACTTCTG 
TGCTTATTGG ATTGGATAAT GGCCTTGCCA TTGAAGACCT TGCTGCAGCC TCTTCACGCT 
ACAGGAGCAG AAAATGATAA GACTGAGAGA TCTGTTCTTA ATTGTATATA TAAGGTTCTA 
CATGGATGTG TCTATGGATC TCAGTGTTTT AGTAACCCCA AATATTTTCC CCTAAGTCTT 
TCTGATTTGG CATGTGTGGA TTACGATCCA TTTATGCATT TAGAAAGCTT GAAGGAGCCA 
GAACCACTGC ATTCTCCAGA CTCAGAGCGT TCTTCTAAAC TTCAACCAGT CACCGAAGTG 
AAAACTCACA TGCAACAAGG ATTAATTTCT GTTGCTGCTC GTACTGTGAT AACGCATCTA 
GTCAACCACT TAGGTCACTA TCCTATGAGT GGAGGACCTG CTATGCTGAC TAGTCAAGTG 
TGTGAAAATA ATGACAATCC ATATAGTGAA AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG 
AACCCAAACC TTCAATTCTT TGTGTTAAAC AATACTTCCT TGGTGTCTTG CATACAAATC 
CAAGCAGAAG ATGACATGCC TGGGGGAGGA CTGTCTGCTG GACTTGCATC TGCTAATTCA 
AATGTCAGAA TTATAGTGCG TGATCTGTCT GGCAAATACT CATGGGATTC AGCCATTCTG 
TATGGACCAT CATCCTTATG TGCATCTTCA CAACAAACAT CTTTTGCCCT TTCATTATCT 
CAACAAGAAA AAACGGAAGA TCCCCTTTCT TCTTTTGAGC ATGTGGAGGA CATATCTGCA 
AGGGATGGAA TTACACTCCA GGTAAAAAGG AAATTCAGAG ACACTGTGCC AACATGGGAT 
ACAATTAGGG ATGAAGAAGA TGCCCTTGAT GAGCTCTTGC AGTATTTAGG TGTTACAAGC 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTCAACATCC CTGCTCCTCA ACCAGTCTGC 
ATCTCAGAGA AGCAGGAGAA TGACGTCATC AATGCAATTC TGAAACAGCA TACAGAAGAG 
AGAGAGTTTG TTGAAAAACA TTTCAATGAT TTAAATATGA GGGCTATGGA GCAGGATGAG 
CCAACATCGC AAAAGCCCCA GTCAGCATTT TACTACTGCA GACTACTTCT CAGTATACTG 
GGAATGAATT CTTGGGATAA AAGAAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAGTTA 
CTTCGAGAGC TCAGAAATTT GGACTCAAGA CAGTGCCGAG AGACCCACAA GATTGCAGTG 
TTTTATGTTG CTGAGGGACA AGAAGATAAA CACTCCATTC TTACTAATAC TGGGGGAAGT 
CAAGCCTATG AGGATTTTGT AGCTGGCCTT GGCTGGGAGG TAAATCTCAC AAACCATTGT 
GGCTTTATGG GAGGACTGCA AAAAAACAAA AGCACTGGAT TGACCACTCC ATATTTTGCT 
ACCTCAACAG TAGAAGTAAT GTTCCATGTG TCAACAAGAA TGCCTTCTGA TTCAGATGAC 
TCTCTAACAA AAAAGCTAAG ACATTTAGGG AACGATGAAG TACACATTGT TTGGTCAGAG 
CATACTCGAG ATTATAGAAG AGGAATAATT CCAACCGAAT TTGGTGATGT TCTTATTGTT 
ATCTATCCCA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAGAAGCC TGAGGTTCCA 
TTTTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAATTCTTCC TATTATGGTT 
CGCGCAACAG CTATAAATGC AAGCCGTGCA TTGAAATCAT TAATCCCTTT ATACCAAAAC 
TTCTATGAGG AGAGAGCACG ATACCTGCAA ACAATTGTTC AGCATCACCT AGAACCTACA 
ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC TGTCCAGCAC CTTACCACCA TTTGCCCTCA 
GATGCAGAAA GAAATGCTGT GATATTTCTT CTCCTTCCAA AACCTCCACT AGAAAGCATT 
GGCCACATCA AGGTAAAGGG CAAAGGCTTG AAACGGAAGG TCTTTCACGC TAGCTGTGAA 
TGGCTCCTGC CCAGAGACTC AGCCGGGAGA GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA 
GGCGGATGGA ACCGACTTAG CCTCACCCAT GTCTCCTCGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287383.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142869.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X16, mRNA.

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CloneID	OGa36827
Clone ID Related Accession (Same CDS sequence)	XM_015287383.1 , XM_015287383.2
Accession Version	XM_015287383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGAAA CTGGTGAATT TCCCTCAGAA TGTTGCAGTG TTATGGCTGG GGGAACACTT 
ACAGGATGGC ACGCAGATGT TGCTACTGTG ATGTGGAGAC GAATGCTAGG AATTTTAGGT 
GATGTCAACA GCATCATGGA TCCAGAGATT CATGCCCAGG TTTTTGATTA CCTGTGTGAA 
CTGTGGCAGA ATCTGGCCAA GATAAGAGAC AATCTTGGTA TTTCAACAGA TAACCTCACT 
TCACCATCTC CACCAGTTTT AATCCCACCA CTGAGAATTC TAACACCTTG GCTGTTCAAG 
GCAACAATGC TGACTGATAA ATACAAACAA GGGAAGCTAC ATGCTTATAA ACTCATTTGT 
AAGACAATGA AGCGAAGACA AGATGTATCT CCAAACAAGG ATTTTTTGAC TCATTTCTAT 
AATATAATGC ACTGTGGACT TCTTCATGTT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCTC CTCAGTTCTT TTCACTTAGT TTGCCTGGTG CCACCATGCT TATTATGGAT 
TTCATTGTAG CAGCAGGAAG GGTGGCAGCT TCTTCTTTTC TCAATGCACC AAGAGTTGAA 
GCTCAAGTTC TTTTAGGATC TCTTGTCTGT TTTCCCAATT TATATCATGA ACTACCAGCT 
CTTCACCCAA ACATTCCTGA CATAGCTGTG TCTCAGTTTA CAGATGTTAA GGAGCTCATA 
ATTAAAACTG TGTTAAGTTC AGCCAGAGAG GAGCCATCTG GTCCTGCAAG ATGTGTTGCT 
CTTTGCAGCC TTGGTATTTG GATCTGTGAG GAGCTAGTTC ATGAATCTCA TCATCCTCAG 
ATCAAGGAAG CATTGAATGT GATTTGTGTT TCTTTAAAGT TTCCGAATAA GACAGTAGCT 
CAAGTTGCCT GCAGTATGCT GAATATGCTG ATACATTATG TGCATAGACT TCAAGTGTAT 
CAAGCTGATT CACCTTTAAG AATTATTCAA ATTCTGATAG CTACAATTAC CCATCTGCTA 
CCCGGTACAG AAGCTTCATC CTATGAACAG GACAAGAGGT TGGTAGTTTC CTTACTTCTG 
TGCTTATTGG ATTGGATAAT GGCCTTGCCA TTGAAGACCT TGCTGCAGCC TCTTCACGCT 
ACAGGAGCAG AAAATGATAA GACTGAGAGA TCTGTTCTTA ATTGTATATA TAAGGTTCTA 
CATGGATGTG TCTATGGATC TCAGTGTTTT AGTAACCCCA AATATTTTCC CCTAAGTCTT 
TCTGATTTGG CATGTGTGGA TTACGATCCA TTTATGCATT TAGAAAGCTT GAAGGAGCCA 
GAACCACTGC ATTCTCCAGA CTCAGAGCGT TCTTCTAAAC TTCAACCAGT CACCGAAGTG 
AAAACTCACA TGCAACAAGG ATTAATTTCT GTTGCTGCTC GTACTGTGAT AACGCATCTA 
GTCAACCACT TAGGTCACTA TCCTATGAGT GGAGGACCTG CTATGCTGAC TAGTCAAGTG 
TGTGAAAATA ATGACAATCC ATATAGTGAA AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG 
AACCCAAACC TTCAATTCTT TGTGTTAAAC AATACTTCCT TGGTGTCTTG CATACAAATC 
CAAGCAGAAG ATGACATGCC TGGGGGAGGA CTGTCTGCTG GACTTGCATC TGCTAATTCA 
AATGTCAGAA TTATAGTGCG TGATCTGTCT GGCAAATACT CATGGGATTC AGCCATTCTG 
TATGGACCAT CATCCTTATG TGCATCTTCA CAACAAACAT CTTTTGCCCT TTCATTATCT 
CAACAAGAAA AAACGGAAGA TCCCCTTTCT TCTTTTGAGC ATGTGGAGGA CATATCTGCA 
AGGGATGGAA TTACACTCCA GGTAAAAAGG AAATTCAGAG ACACTGTGCC AACATGGGAT 
ACAATTAGGG ATGAAGAAGA TGCCCTTGAT GAGCTCTTGC AGTATTTAGG TGTTACAAGC 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTCAACATCC CTGCTCCTCA ACCAGTCTGC 
ATCTCAGAGA AGCAGGAGAA TGACGTCATC AATGCAATTC TGAAACAGCA TACAGAAGAG 
AGAGAGTTTG TTGAAAAACA TTTCAATGAT TTAAATATGA GGGCTATGGA GCAGGATGAG 
CCAACATCGC AAAAGCCCCA GTCAGCATTT TACTACTGCA GACTACTTCT CAGTATACTG 
GGAATGAATT CTTGGGATAA AAGAAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAGTTA 
CTTCGAGAGC TCAGAAATTT GGACTCAAGA CAGTGCCGAG AGACCCACAA GATTGCAGTG 
TTTTATGTTG CTGAGGGACA AGAAGATAAA CACTCCATTC TTACTAATAC TGGGGGAAGT 
CAAGCCTATG AGGATTTTGT AGCTGGCCTT GGCTGGGAGG TAAATCTCAC AAACCATTGT 
GGCTTTATGG GAGGACTGCA AAAAAACAAA AGCACTGGAT TGACCACTCC ATATTTTGCT 
ACCTCAACAG TAGAAGTAAT GTTCCATGTG TCAACAAGAA TGCCTTCTGA TTCAGATGAC 
TCTCTAACAA AAAAGCTAAG ACATTTAGGG AACGATGAAG TACACATTGT TTGGTCAGAG 
CATACTCGAG ATTATAGAAG AGGAATAATT CCAACCGAAT TTGGTGATGT TCTTATTGTT 
ATCTATCCCA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAGAAGCC TGAGGTTCCA 
TTTTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAATTCTTCC TATTATGGTT 
CGCGCAACAG CTATAAATGC AAGCCGTGCA TTGAAATCAT TAATCCCTTT ATACCAAAAC 
TTCTATGAGG AGAGAGCACG ATACCTGCAA ACAATTGTTC AGCATCACCT AGAACCTACA 
ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC TGTCCAGCAC CTTACCACCA TTTGCCCTCA 
GATGCAGAAA GAAATGCTGT GATATTTCTT CTCCTTCCAA AACCTCCACT AGAAAGCATT 
GGCCACATCA AGGTAAAGGG CAAAGGCTTG AAACGGAAGG TCTTTCACGC TAGCTGTGAA 
TGGCTCCTGC CCAGAGACTC AGCCGGGAGA GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA 
GGCGGATGGA ACCGACTTAG CCTCACCCAT GTCTCCTCGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142869.1
CDS	1..6165
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287383.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287383.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGAAA CTGGTGAATT TCCCTCAGAA TGTTGCAGTG TTATGGCTGG GGGAACACTT 
ACAGGATGGC ACGCAGATGT TGCTACTGTG ATGTGGAGAC GAATGCTAGG AATTTTAGGT 
GATGTCAACA GCATCATGGA TCCAGAGATT CATGCCCAGG TTTTTGATTA CCTGTGTGAA 
CTGTGGCAGA ATCTGGCCAA GATAAGAGAC AATCTTGGTA TTTCAACAGA TAACCTCACT 
TCACCATCTC CACCAGTTTT AATCCCACCA CTGAGAATTC TAACACCTTG GCTGTTCAAG 
GCAACAATGC TGACTGATAA ATACAAACAA GGGAAGCTAC ATGCTTATAA ACTCATTTGT 
AAGACAATGA AGCGAAGACA AGATGTATCT CCAAACAAGG ATTTTTTGAC TCATTTCTAT 
AATATAATGC ACTGTGGACT TCTTCATGTT GATCAGGATA TTGTCAATAC AATCATCAAG 
CACTGCTCTC CTCAGTTCTT TTCACTTAGT TTGCCTGGTG CCACCATGCT TATTATGGAT 
TTCATTGTAG CAGCAGGAAG GGTGGCAGCT TCTTCTTTTC TCAATGCACC AAGAGTTGAA 
GCTCAAGTTC TTTTAGGATC TCTTGTCTGT TTTCCCAATT TATATCATGA ACTACCAGCT 
CTTCACCCAA ACATTCCTGA CATAGCTGTG TCTCAGTTTA CAGATGTTAA GGAGCTCATA 
ATTAAAACTG TGTTAAGTTC AGCCAGAGAG GAGCCATCTG GTCCTGCAAG ATGTGTTGCT 
CTTTGCAGCC TTGGTATTTG GATCTGTGAG GAGCTAGTTC ATGAATCTCA TCATCCTCAG 
ATCAAGGAAG CATTGAATGT GATTTGTGTT TCTTTAAAGT TTCCGAATAA GACAGTAGCT 
CAAGTTGCCT GCAGTATGCT GAATATGCTG ATACATTATG TGCATAGACT TCAAGTGTAT 
CAAGCTGATT CACCTTTAAG AATTATTCAA ATTCTGATAG CTACAATTAC CCATCTGCTA 
CCCGGTACAG AAGCTTCATC CTATGAACAG GACAAGAGGT TGGTAGTTTC CTTACTTCTG 
TGCTTATTGG ATTGGATAAT GGCCTTGCCA TTGAAGACCT TGCTGCAGCC TCTTCACGCT 
ACAGGAGCAG AAAATGATAA GACTGAGAGA TCTGTTCTTA ATTGTATATA TAAGGTTCTA 
CATGGATGTG TCTATGGATC TCAGTGTTTT AGTAACCCCA AATATTTTCC CCTAAGTCTT 
TCTGATTTGG CATGTGTGGA TTACGATCCA TTTATGCATT TAGAAAGCTT GAAGGAGCCA 
GAACCACTGC ATTCTCCAGA CTCAGAGCGT TCTTCTAAAC TTCAACCAGT CACCGAAGTG 
AAAACTCACA TGCAACAAGG ATTAATTTCT GTTGCTGCTC GTACTGTGAT AACGCATCTA 
GTCAACCACT TAGGTCACTA TCCTATGAGT GGAGGACCTG CTATGCTGAC TAGTCAAGTG 
TGTGAAAATA ATGACAATCC ATATAGTGAA AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG 
AACCCAAACC TTCAATTCTT TGTGTTAAAC AATACTTCCT TGGTGTCTTG CATACAAATC 
CAAGCAGAAG ATGACATGCC TGGGGGAGGA CTGTCTGCTG GACTTGCATC TGCTAATTCA 
AATGTCAGAA TTATAGTGCG TGATCTGTCT GGCAAATACT CATGGGATTC AGCCATTCTG 
TATGGACCAT CATCCTTATG TGCATCTTCA CAACAAACAT CTTTTGCCCT TTCATTATCT 
CAACAAGAAA AAACGGAAGA TCCCCTTTCT TCTTTTGAGC ATGTGGAGGA CATATCTGCA 
AGGGATGGAA TTACACTCCA GGTAAAAAGG AAATTCAGAG ACACTGTGCC AACATGGGAT 
ACAATTAGGG ATGAAGAAGA TGCCCTTGAT GAGCTCTTGC AGTATTTAGG TGTTACAAGC 
CCTGAATGCT TACAGAGAAC TGGAGTCTCA CTCAACATCC CTGCTCCTCA ACCAGTCTGC 
ATCTCAGAGA AGCAGGAGAA TGACGTCATC AATGCAATTC TGAAACAGCA TACAGAAGAG 
AGAGAGTTTG TTGAAAAACA TTTCAATGAT TTAAATATGA GGGCTATGGA GCAGGATGAG 
CCAACATCGC AAAAGCCCCA GTCAGCATTT TACTACTGCA GACTACTTCT CAGTATACTG 
GGAATGAATT CTTGGGATAA AAGAAGGAGC TTTCATCTCC TGAAGAAAAA TGAAAAGTTA 
CTTCGAGAGC TCAGAAATTT GGACTCAAGA CAGTGCCGAG AGACCCACAA GATTGCAGTG 
TTTTATGTTG CTGAGGGACA AGAAGATAAA CACTCCATTC TTACTAATAC TGGGGGAAGT 
CAAGCCTATG AGGATTTTGT AGCTGGCCTT GGCTGGGAGG TAAATCTCAC AAACCATTGT 
GGCTTTATGG GAGGACTGCA AAAAAACAAA AGCACTGGAT TGACCACTCC ATATTTTGCT 
ACCTCAACAG TAGAAGTAAT GTTCCATGTG TCAACAAGAA TGCCTTCTGA TTCAGATGAC 
TCTCTAACAA AAAAGCTAAG ACATTTAGGG AACGATGAAG TACACATTGT TTGGTCAGAG 
CATACTCGAG ATTATAGAAG AGGAATAATT CCAACCGAAT TTGGTGATGT TCTTATTGTT 
ATCTATCCCA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAGAAGCC TGAGGTTCCA 
TTTTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAATTCTTCC TATTATGGTT 
CGCGCAACAG CTATAAATGC AAGCCGTGCA TTGAAATCAT TAATCCCTTT ATACCAAAAC 
TTCTATGAGG AGAGAGCACG ATACCTGCAA ACAATTGTTC AGCATCACCT AGAACCTACA 
ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC TGTCCAGCAC CTTACCACCA TTTGCCCTCA 
GATGCAGAAA GAAATGCTGT GATATTTCTT CTCCTTCCAA AACCTCCACT AGAAAGCATT 
GGCCACATCA AGGTAAAGGG CAAAGGCTTG AAACGGAAGG TCTTTCACGC TAGCTGTGAA 
TGGCTCCTGC CCAGAGACTC AGCCGGGAGA GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA 
GGCGGATGGA ACCGACTTAG CCTCACCCAT GTCTCCTCGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( NM_001277447.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264376.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 1, mRNA.

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CloneID	OGa23826
Clone ID Related Accession (Same CDS sequence)	NM_001277447.2 , NM_001277447.1
Accession Version	NM_001277447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000303.1. On Dec 20, 2018 this sequence version replaced NM_001277447.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330259.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AGTACACTTA CAGCGACTAA GCAGAATACA 
GGACATGCAA TAGGAATGTC TTCTCCAGAA GTTCAGTCGC AGTTTTGTGA CAGAAATGAG 
AATGTCCATA ATAGCCAATT ACTTTCTGCT TTTACATCTT ATGTAAATTG TGCAACAGTG 
TGTTGGAAGC ATAGGAGATG TACATACAAG TTTTACATGG AGGATTTTGG AGATTTCAGC 
TTTGGCACAG AGGCAGGGAC GCCAGCTTCC ACTGATGCTG ATGCAATTTC AGGACAGCAG 
CAGAGTGCTG AGGAACAAGA AGTAGCCAGT CTAACTACAC TCCACATAGA TTCGGAAACA 
AGTAGTCTGA ATCAGCAACC GTTGTCTGCT GAAGCTGCAA CTGTTACTGG CTCTGAAAGT 
GCTTCTCCAA TTCAATCTGC TCTTGGATCC CGATCACAGA CACCCTCTCC TGCCACTCTT 
CATGCAGATC ATATTGAGCA GAAGGATCTG CAACTGGATG AGAAGCTCCA CCACTCTGTT 
TTGCAGACGC CAGATGACCT AGAAACTGGT GAATTTCCCT CAGAATGTTG CAGTGTTATG 
GCTGGGGGAA CACTTACAGG ATGGCACGCA GATGTTGCTA CTGTGATGTG GAGACGAATG 
CTAGGAATTT TAGGTGATGT CAACAGCATC ATGGATCCAG AGATTCATGC CCAGGTTTTT 
GATTACCTGT GTGAACTGTG GCAGAATCTG GCCAAGATAA GAGACAATCT TGGTATTTCA 
ACAGATAACC TCACTTCACC ATCTCCACCA GTTTTAATCC CACCACTGAG AATTCTAACA 
CCTTGGCTGT TCAAGGCAAC AATGCTGACT GATAAATACA AACAAGGGAA GCTACATGCT 
TATAAACTCA TTTGTAAGAC AATGAAGCGA AGACAAGATG TATCTCCAAA CAAGGATTTT 
TTGACTCATT TCTATAATAT AATGCACTGT GGACTTCTTC ATGTTGATCA GGATATTGTC 
AATACAATCA TCAAGCACTG CTCTCCTCAG TTCTTTTCAC TTAGTTTGCC TGGTGCCACC 
ATGCTTATTA TGGATTTCAT TGTAGCAGCA GGAAGGGTGG CAGCTTCTTC TTTTCTCAAT 
GCACCAAGAG TTGAAGCTCA AGTTCTTTTA GGATCTCTTG TCTGTTTTCC CAATTTATAT 
CATGAACTAC CAGCTCTTCA CCCAAACATT CCTGACATAG CTGTGTCTCA GTTTACAGAT 
GTTAAGGAGC TCATAATTAA AACTGTGTTA AGTTCAGCCA GAGAGGAGCC ATCTGGTCCT 
GCAAGATGTG TTGCTCTTTG CAGCCTTGGT ATTTGGATCT GTGAGGAGCT AGTTCATGAA 
TCTCATCATC CTCAGATCAA GGAAGCATTG AATGTGATTT GTGTTTCTTT AAAGTTTCCG 
AATAAGACAG TAGCTCAAGT TGCCTGCAGT ATGCTGAATA TGCTGATACA TTATGTGCAT 
AGACTTCAAG TGTATCAAGC TGATTCACCT TTAAGAATTA TTCAAATTCT GATAGCTACA 
ATTACCCATC TGCTACCCGG TACAGAAGCT TCATCCTATG AACAGGACAA GAGGTTGGTA 
GTTTCCTTAC TTCTGTGCTT ATTGGATTGG ATAATGGCCT TGCCATTGAA GACCTTGCTG 
CAGCCTCTTC ACGCTACAGG AGCAGAAAAT GATAAGACTG AGAGATCTGT TCTTAATTGT 
ATATATAAGG TTCTACATGG ATGTGTCTAT GGATCTCAGT GTTTTAGTAA CCCCAAATAT 
TTTCCCCTAA GTCTTTCTGA TTTGGCATGT GTGGATTACG ATCCATTTAT GCATTTAGAA 
AGCTTGAAGG AGCCAGAACC ACTGCATTCT CCAGACTCAG AGCGTTCTTC TAAACTTCAA 
CCAGTCACCG AAGTGAAAAC TCACATGCAA CAAGGATTAA TTTCTGTTGC TGCTCGTACT 
GTGATAACGC ATCTAGTCAA CCACTTAGGT CACTATCCTA TGAGTGGAGG ACCTGCTATG 
CTGACTAGTC AAGTGTGTGA AAATAATGAC AATCCATATA GTGAAAGTCC TGAACTTTCT 
CCTGAACTTT TTGAGAACCC AAACCTTCAA TTCTTTGTGT TAAACAATAC TTCCTTGGTG 
TCTTGCATAC AAATCCAAGC AGAAGATGAC ATGCCTGGGG GAGGACTGTC TGCTGGACTT 
GCATCTGCTA ATTCAAATGT CAGAATTATA GTGCGTGATC TGTCTGGCAA ATACTCATGG 
GATTCAGCCA TTCTGTATGG ACCATCATCC TTATGTGCAT CTTCACAACA AACATCTTTT 
GCCCTTTCAT TATCTCAACA AGAAAAAACG GAAGATCCCC TTTCTTCTTT TGAGCATGTG 
GAGGACATAT CTGCAAGGGA TGGAATTACA CTCCAGGTAA AAAGGAAATT CAGAGACACT 
GTGCCAACAT GGGATACAAT TAGGGATGAA GAAGATGCCC TTGATGAGCT CTTGCAGTAT 
TTAGGTGTTA CAAGCCCTGA ATGCTTACAG AGAACTGGAG TCTCACTCAA CATCCCTGCT 
CCTCAACCAG TCTGCATCTC AGAGAAGCAG GAGAATGACG TCATCAATGC AATTCTGAAA 
CAGCATACAG AAGAGAGAGA GTTTGTTGAA AAACATTTCA ATGATTTAAA TATGAGGGCT 
ATGGAGCAGG ATGAGCCAAC ATCGCAAAAG CCCCAGTCAG CATTTTACTA CTGCAGACTA 
CTTCTCAGTA TACTGGGAAT GAATTCTTGG GATAAAAGAA GGAGCTTTCA TCTCCTGAAG 
AAAAATGAAA AGTTACTTCG AGAGCTCAGA AATTTGGACT CAAGACAGTG CCGAGAGACC 
CACAAGATTG CAGTGTTTTA TGTTGCTGAG GGACAAGAAG ATAAACACTC CATTCTTACT 
AATACTGGGG GAAGTCAAGC CTATGAGGAT TTTGTAGCTG GCCTTGGCTG GGAGGTAAAT 
CTCACAAACC ATTGTGGCTT TATGGGAGGA CTGCAAAAAA ACAAAAGCAC TGGATTGACC 
ACTCCATATT TTGCTACCTC AACAGTAGAA GTAATGTTCC ATGTGTCAAC AAGAATGCCT 
TCTGATTCAG ATGACTCTCT AACAAAAAAG CTAAGACATT TAGGGAACGA TGAAGTACAC 
ATTGTTTGGT CAGAGCATAC TCGAGATTAT AGAAGAGGAA TAATTCCAAC CGAATTTGGT 
GATGTTCTTA TTGTTATCTA TCCCATGAAA AACCACATGT TCAGTATCCA GATCATGAAG 
AAGCCTGAGG TTCCATTTTT TGGTCCACTT TTTGATGGTG CTATTGTGAA TGGAAAAATT 
CTTCCTATTA TGGTTCGCGC AACAGCTATA AATGCAAGCC GTGCATTGAA ATCATTAATC 
CCTTTATACC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTTCAGCAT 
CACCTAGAAC CTACAACTTT TGAGGATTTT GCTGCTCAGG TTTTCTGTCC AGCACCTTAC 
CACCATTTGC CCTCAGATGC AGTTGGCTCC TGCCCAGAGA CTCAGCCGGG AGAGCCTCCA 
GCAGCAGCAG CAGCAGCAGC GCAGGCGGAT GGAACCGACT TAGCCTCACC CATGTCTCCT 
CGGACTAGCA AGAGCCGCAT GTCCATGAAG CTGCGGCGCT CCTCGGGCTC GGCCAACAAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264376.1
CDS	217..6822
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAVGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	NM_001277447.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277447.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AGTACACTTA CAGCGACTAA GCAGAATACA 
GGACATGCAA TAGGAATGTC TTCTCCAGAA GTTCAGTCGC AGTTTTGTGA CAGAAATGAG 
AATGTCCATA ATAGCCAATT ACTTTCTGCT TTTACATCTT ATGTAAATTG TGCAACAGTG 
TGTTGGAAGC ATAGGAGATG TACATACAAG TTTTACATGG AGGATTTTGG AGATTTCAGC 
TTTGGCACAG AGGCAGGGAC GCCAGCTTCC ACTGATGCTG ATGCAATTTC AGGACAGCAG 
CAGAGTGCTG AGGAACAAGA AGTAGCCAGT CTAACTACAC TCCACATAGA TTCGGAAACA 
AGTAGTCTGA ATCAGCAACC GTTGTCTGCT GAAGCTGCAA CTGTTACTGG CTCTGAAAGT 
GCTTCTCCAA TTCAATCTGC TCTTGGATCC CGATCACAGA CACCCTCTCC TGCCACTCTT 
CATGCAGATC ATATTGAGCA GAAGGATCTG CAACTGGATG AGAAGCTCCA CCACTCTGTT 
TTGCAGACGC CAGATGACCT AGAAACTGGT GAATTTCCCT CAGAATGTTG CAGTGTTATG 
GCTGGGGGAA CACTTACAGG ATGGCACGCA GATGTTGCTA CTGTGATGTG GAGACGAATG 
CTAGGAATTT TAGGTGATGT CAACAGCATC ATGGATCCAG AGATTCATGC CCAGGTTTTT 
GATTACCTGT GTGAACTGTG GCAGAATCTG GCCAAGATAA GAGACAATCT TGGTATTTCA 
ACAGATAACC TCACTTCACC ATCTCCACCA GTTTTAATCC CACCACTGAG AATTCTAACA 
CCTTGGCTGT TCAAGGCAAC AATGCTGACT GATAAATACA AACAAGGGAA GCTACATGCT 
TATAAACTCA TTTGTAAGAC AATGAAGCGA AGACAAGATG TATCTCCAAA CAAGGATTTT 
TTGACTCATT TCTATAATAT AATGCACTGT GGACTTCTTC ATGTTGATCA GGATATTGTC 
AATACAATCA TCAAGCACTG CTCTCCTCAG TTCTTTTCAC TTAGTTTGCC TGGTGCCACC 
ATGCTTATTA TGGATTTCAT TGTAGCAGCA GGAAGGGTGG CAGCTTCTTC TTTTCTCAAT 
GCACCAAGAG TTGAAGCTCA AGTTCTTTTA GGATCTCTTG TCTGTTTTCC CAATTTATAT 
CATGAACTAC CAGCTCTTCA CCCAAACATT CCTGACATAG CTGTGTCTCA GTTTACAGAT 
GTTAAGGAGC TCATAATTAA AACTGTGTTA AGTTCAGCCA GAGAGGAGCC ATCTGGTCCT 
GCAAGATGTG TTGCTCTTTG CAGCCTTGGT ATTTGGATCT GTGAGGAGCT AGTTCATGAA 
TCTCATCATC CTCAGATCAA GGAAGCATTG AATGTGATTT GTGTTTCTTT AAAGTTTCCG 
AATAAGACAG TAGCTCAAGT TGCCTGCAGT ATGCTGAATA TGCTGATACA TTATGTGCAT 
AGACTTCAAG TGTATCAAGC TGATTCACCT TTAAGAATTA TTCAAATTCT GATAGCTACA 
ATTACCCATC TGCTACCCGG TACAGAAGCT TCATCCTATG AACAGGACAA GAGGTTGGTA 
GTTTCCTTAC TTCTGTGCTT ATTGGATTGG ATAATGGCCT TGCCATTGAA GACCTTGCTG 
CAGCCTCTTC ACGCTACAGG AGCAGAAAAT GATAAGACTG AGAGATCTGT TCTTAATTGT 
ATATATAAGG TTCTACATGG ATGTGTCTAT GGATCTCAGT GTTTTAGTAA CCCCAAATAT 
TTTCCCCTAA GTCTTTCTGA TTTGGCATGT GTGGATTACG ATCCATTTAT GCATTTAGAA 
AGCTTGAAGG AGCCAGAACC ACTGCATTCT CCAGACTCAG AGCGTTCTTC TAAACTTCAA 
CCAGTCACCG AAGTGAAAAC TCACATGCAA CAAGGATTAA TTTCTGTTGC TGCTCGTACT 
GTGATAACGC ATCTAGTCAA CCACTTAGGT CACTATCCTA TGAGTGGAGG ACCTGCTATG 
CTGACTAGTC AAGTGTGTGA AAATAATGAC AATCCATATA GTGAAAGTCC TGAACTTTCT 
CCTGAACTTT TTGAGAACCC AAACCTTCAA TTCTTTGTGT TAAACAATAC TTCCTTGGTG 
TCTTGCATAC AAATCCAAGC AGAAGATGAC ATGCCTGGGG GAGGACTGTC TGCTGGACTT 
GCATCTGCTA ATTCAAATGT CAGAATTATA GTGCGTGATC TGTCTGGCAA ATACTCATGG 
GATTCAGCCA TTCTGTATGG ACCATCATCC TTATGTGCAT CTTCACAACA AACATCTTTT 
GCCCTTTCAT TATCTCAACA AGAAAAAACG GAAGATCCCC TTTCTTCTTT TGAGCATGTG 
GAGGACATAT CTGCAAGGGA TGGAATTACA CTCCAGGTAA AAAGGAAATT CAGAGACACT 
GTGCCAACAT GGGATACAAT TAGGGATGAA GAAGATGCCC TTGATGAGCT CTTGCAGTAT 
TTAGGTGTTA CAAGCCCTGA ATGCTTACAG AGAACTGGAG TCTCACTCAA CATCCCTGCT 
CCTCAACCAG TCTGCATCTC AGAGAAGCAG GAGAATGACG TCATCAATGC AATTCTGAAA 
CAGCATACAG AAGAGAGAGA GTTTGTTGAA AAACATTTCA ATGATTTAAA TATGAGGGCT 
ATGGAGCAGG ATGAGCCAAC ATCGCAAAAG CCCCAGTCAG CATTTTACTA CTGCAGACTA 
CTTCTCAGTA TACTGGGAAT GAATTCTTGG GATAAAAGAA GGAGCTTTCA TCTCCTGAAG 
AAAAATGAAA AGTTACTTCG AGAGCTCAGA AATTTGGACT CAAGACAGTG CCGAGAGACC 
CACAAGATTG CAGTGTTTTA TGTTGCTGAG GGACAAGAAG ATAAACACTC CATTCTTACT 
AATACTGGGG GAAGTCAAGC CTATGAGGAT TTTGTAGCTG GCCTTGGCTG GGAGGTAAAT 
CTCACAAACC ATTGTGGCTT TATGGGAGGA CTGCAAAAAA ACAAAAGCAC TGGATTGACC 
ACTCCATATT TTGCTACCTC AACAGTAGAA GTAATGTTCC ATGTGTCAAC AAGAATGCCT 
TCTGATTCAG ATGACTCTCT AACAAAAAAG CTAAGACATT TAGGGAACGA TGAAGTACAC 
ATTGTTTGGT CAGAGCATAC TCGAGATTAT AGAAGAGGAA TAATTCCAAC CGAATTTGGT 
GATGTTCTTA TTGTTATCTA TCCCATGAAA AACCACATGT TCAGTATCCA GATCATGAAG 
AAGCCTGAGG TTCCATTTTT TGGTCCACTT TTTGATGGTG CTATTGTGAA TGGAAAAATT 
CTTCCTATTA TGGTTCGCGC AACAGCTATA AATGCAAGCC GTGCATTGAA ATCATTAATC 
CCTTTATACC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTTCAGCAT 
CACCTAGAAC CTACAACTTT TGAGGATTTT GCTGCTCAGG TTTTCTGTCC AGCACCTTAC 
CACCATTTGC CCTCAGATGC AGTTGGCTCC TGCCCAGAGA CTCAGCCGGG AGAGCCTCCA 
GCAGCAGCAG CAGCAGCAGC GCAGGCGGAT GGAACCGACT TAGCCTCACC CATGTCTCCT 
CGGACTAGCA AGAGCCGCAT GTCCATGAAG CTGCGGCGCT CCTCGGGCTC GGCCAACAAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( NM_001277447.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264376.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 1, mRNA.

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CloneID	OGa23826
Clone ID Related Accession (Same CDS sequence)	NM_001277447.2 , NM_001277447.1
Accession Version	NM_001277447.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-05
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04000014.1. On or before Apr 10, 2013 this sequence version replaced XM_421244.2, XM_003641293.1, XM_003641294.1, XM_003641295.1, XM_003641296.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330259.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AGTACACTTA CAGCGACTAA GCAGAATACA 
GGACATGCAA TAGGAATGTC TTCTCCAGAA GTTCAGTCGC AGTTTTGTGA CAGAAATGAG 
AATGTCCATA ATAGCCAATT ACTTTCTGCT TTTACATCTT ATGTAAATTG TGCAACAGTG 
TGTTGGAAGC ATAGGAGATG TACATACAAG TTTTACATGG AGGATTTTGG AGATTTCAGC 
TTTGGCACAG AGGCAGGGAC GCCAGCTTCC ACTGATGCTG ATGCAATTTC AGGACAGCAG 
CAGAGTGCTG AGGAACAAGA AGTAGCCAGT CTAACTACAC TCCACATAGA TTCGGAAACA 
AGTAGTCTGA ATCAGCAACC GTTGTCTGCT GAAGCTGCAA CTGTTACTGG CTCTGAAAGT 
GCTTCTCCAA TTCAATCTGC TCTTGGATCC CGATCACAGA CACCCTCTCC TGCCACTCTT 
CATGCAGATC ATATTGAGCA GAAGGATCTG CAACTGGATG AGAAGCTCCA CCACTCTGTT 
TTGCAGACGC CAGATGACCT AGAAACTGGT GAATTTCCCT CAGAATGTTG CAGTGTTATG 
GCTGGGGGAA CACTTACAGG ATGGCACGCA GATGTTGCTA CTGTGATGTG GAGACGAATG 
CTAGGAATTT TAGGTGATGT CAACAGCATC ATGGATCCAG AGATTCATGC CCAGGTTTTT 
GATTACCTGT GTGAACTGTG GCAGAATCTG GCCAAGATAA GAGACAATCT TGGTATTTCA 
ACAGATAACC TCACTTCACC ATCTCCACCA GTTTTAATCC CACCACTGAG AATTCTAACA 
CCTTGGCTGT TCAAGGCAAC AATGCTGACT GATAAATACA AACAAGGGAA GCTACATGCT 
TATAAACTCA TTTGTAAGAC AATGAAGCGA AGACAAGATG TATCTCCAAA CAAGGATTTT 
TTGACTCATT TCTATAATAT AATGCACTGT GGACTTCTTC ATGTTGATCA GGATATTGTC 
AATACAATCA TCAAGCACTG CTCTCCTCAG TTCTTTTCAC TTAGTTTGCC TGGTGCCACC 
ATGCTTATTA TGGATTTCAT TGTAGCAGCA GGAAGGGTGG CAGCTTCTTC TTTTCTCAAT 
GCACCAAGAG TTGAAGCTCA AGTTCTTTTA GGATCTCTTG TCTGTTTTCC CAATTTATAT 
CATGAACTAC CAGCTCTTCA CCCAAACATT CCTGACATAG CTGTGTCTCA GTTTACAGAT 
GTTAAGGAGC TCATAATTAA AACTGTGTTA AGTTCAGCCA GAGAGGAGCC ATCTGGTCCT 
GCAAGATGTG TTGCTCTTTG CAGCCTTGGT ATTTGGATCT GTGAGGAGCT AGTTCATGAA 
TCTCATCATC CTCAGATCAA GGAAGCATTG AATGTGATTT GTGTTTCTTT AAAGTTTCCG 
AATAAGACAG TAGCTCAAGT TGCCTGCAGT ATGCTGAATA TGCTGATACA TTATGTGCAT 
AGACTTCAAG TGTATCAAGC TGATTCACCT TTAAGAATTA TTCAAATTCT GATAGCTACA 
ATTACCCATC TGCTACCCGG TACAGAAGCT TCATCCTATG AACAGGACAA GAGGTTGGTA 
GTTTCCTTAC TTCTGTGCTT ATTGGATTGG ATAATGGCCT TGCCATTGAA GACCTTGCTG 
CAGCCTCTTC ACGCTACAGG AGCAGAAAAT GATAAGACTG AGAGATCTGT TCTTAATTGT 
ATATATAAGG TTCTACATGG ATGTGTCTAT GGATCTCAGT GTTTTAGTAA CCCCAAATAT 
TTTCCCCTAA GTCTTTCTGA TTTGGCATGT GTGGATTACG ATCCATTTAT GCATTTAGAA 
AGCTTGAAGG AGCCAGAACC ACTGCATTCT CCAGACTCAG AGCGTTCTTC TAAACTTCAA 
CCAGTCACCG AAGTGAAAAC TCACATGCAA CAAGGATTAA TTTCTGTTGC TGCTCGTACT 
GTGATAACGC ATCTAGTCAA CCACTTAGGT CACTATCCTA TGAGTGGAGG ACCTGCTATG 
CTGACTAGTC AAGTGTGTGA AAATAATGAC AATCCATATA GTGAAAGTCC TGAACTTTCT 
CCTGAACTTT TTGAGAACCC AAACCTTCAA TTCTTTGTGT TAAACAATAC TTCCTTGGTG 
TCTTGCATAC AAATCCAAGC AGAAGATGAC ATGCCTGGGG GAGGACTGTC TGCTGGACTT 
GCATCTGCTA ATTCAAATGT CAGAATTATA GTGCGTGATC TGTCTGGCAA ATACTCATGG 
GATTCAGCCA TTCTGTATGG ACCATCATCC TTATGTGCAT CTTCACAACA AACATCTTTT 
GCCCTTTCAT TATCTCAACA AGAAAAAACG GAAGATCCCC TTTCTTCTTT TGAGCATGTG 
GAGGACATAT CTGCAAGGGA TGGAATTACA CTCCAGGTAA AAAGGAAATT CAGAGACACT 
GTGCCAACAT GGGATACAAT TAGGGATGAA GAAGATGCCC TTGATGAGCT CTTGCAGTAT 
TTAGGTGTTA CAAGCCCTGA ATGCTTACAG AGAACTGGAG TCTCACTCAA CATCCCTGCT 
CCTCAACCAG TCTGCATCTC AGAGAAGCAG GAGAATGACG TCATCAATGC AATTCTGAAA 
CAGCATACAG AAGAGAGAGA GTTTGTTGAA AAACATTTCA ATGATTTAAA TATGAGGGCT 
ATGGAGCAGG ATGAGCCAAC ATCGCAAAAG CCCCAGTCAG CATTTTACTA CTGCAGACTA 
CTTCTCAGTA TACTGGGAAT GAATTCTTGG GATAAAAGAA GGAGCTTTCA TCTCCTGAAG 
AAAAATGAAA AGTTACTTCG AGAGCTCAGA AATTTGGACT CAAGACAGTG CCGAGAGACC 
CACAAGATTG CAGTGTTTTA TGTTGCTGAG GGACAAGAAG ATAAACACTC CATTCTTACT 
AATACTGGGG GAAGTCAAGC CTATGAGGAT TTTGTAGCTG GCCTTGGCTG GGAGGTAAAT 
CTCACAAACC ATTGTGGCTT TATGGGAGGA CTGCAAAAAA ACAAAAGCAC TGGATTGACC 
ACTCCATATT TTGCTACCTC AACAGTAGAA GTAATGTTCC ATGTGTCAAC AAGAATGCCT 
TCTGATTCAG ATGACTCTCT AACAAAAAAG CTAAGACATT TAGGGAACGA TGAAGTACAC 
ATTGTTTGGT CAGAGCATAC TCGAGATTAT AGAAGAGGAA TAATTCCAAC CGAATTTGGT 
GATGTTCTTA TTGTTATCTA TCCCATGAAA AACCACATGT TCAGTATCCA GATCATGAAG 
AAGCCTGAGG TTCCATTTTT TGGTCCACTT TTTGATGGTG CTATTGTGAA TGGAAAAATT 
CTTCCTATTA TGGTTCGCGC AACAGCTATA AATGCAAGCC GTGCATTGAA ATCATTAATC 
CCTTTATACC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTTCAGCAT 
CACCTAGAAC CTACAACTTT TGAGGATTTT GCTGCTCAGG TTTTCTGTCC AGCACCTTAC 
CACCATTTGC CCTCAGATGC AGTTGGCTCC TGCCCAGAGA CTCAGCCGGG AGAGCCTCCA 
GCAGCAGCAG CAGCAGCAGC GCAGGCGGAT GGAACCGACT TAGCCTCACC CATGTCTCCT 
CGGACTAGCA AGAGCCGCAT GTCCATGAAG CTGCGGCGCT CCTCGGGCTC GGCCAACAAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264376.1
CDS	217..6822
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAVGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	NM_001277447.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277447.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AGTACACTTA CAGCGACTAA GCAGAATACA 
GGACATGCAA TAGGAATGTC TTCTCCAGAA GTTCAGTCGC AGTTTTGTGA CAGAAATGAG 
AATGTCCATA ATAGCCAATT ACTTTCTGCT TTTACATCTT ATGTAAATTG TGCAACAGTG 
TGTTGGAAGC ATAGGAGATG TACATACAAG TTTTACATGG AGGATTTTGG AGATTTCAGC 
TTTGGCACAG AGGCAGGGAC GCCAGCTTCC ACTGATGCTG ATGCAATTTC AGGACAGCAG 
CAGAGTGCTG AGGAACAAGA AGTAGCCAGT CTAACTACAC TCCACATAGA TTCGGAAACA 
AGTAGTCTGA ATCAGCAACC GTTGTCTGCT GAAGCTGCAA CTGTTACTGG CTCTGAAAGT 
GCTTCTCCAA TTCAATCTGC TCTTGGATCC CGATCACAGA CACCCTCTCC TGCCACTCTT 
CATGCAGATC ATATTGAGCA GAAGGATCTG CAACTGGATG AGAAGCTCCA CCACTCTGTT 
TTGCAGACGC CAGATGACCT AGAAACTGGT GAATTTCCCT CAGAATGTTG CAGTGTTATG 
GCTGGGGGAA CACTTACAGG ATGGCACGCA GATGTTGCTA CTGTGATGTG GAGACGAATG 
CTAGGAATTT TAGGTGATGT CAACAGCATC ATGGATCCAG AGATTCATGC CCAGGTTTTT 
GATTACCTGT GTGAACTGTG GCAGAATCTG GCCAAGATAA GAGACAATCT TGGTATTTCA 
ACAGATAACC TCACTTCACC ATCTCCACCA GTTTTAATCC CACCACTGAG AATTCTAACA 
CCTTGGCTGT TCAAGGCAAC AATGCTGACT GATAAATACA AACAAGGGAA GCTACATGCT 
TATAAACTCA TTTGTAAGAC AATGAAGCGA AGACAAGATG TATCTCCAAA CAAGGATTTT 
TTGACTCATT TCTATAATAT AATGCACTGT GGACTTCTTC ATGTTGATCA GGATATTGTC 
AATACAATCA TCAAGCACTG CTCTCCTCAG TTCTTTTCAC TTAGTTTGCC TGGTGCCACC 
ATGCTTATTA TGGATTTCAT TGTAGCAGCA GGAAGGGTGG CAGCTTCTTC TTTTCTCAAT 
GCACCAAGAG TTGAAGCTCA AGTTCTTTTA GGATCTCTTG TCTGTTTTCC CAATTTATAT 
CATGAACTAC CAGCTCTTCA CCCAAACATT CCTGACATAG CTGTGTCTCA GTTTACAGAT 
GTTAAGGAGC TCATAATTAA AACTGTGTTA AGTTCAGCCA GAGAGGAGCC ATCTGGTCCT 
GCAAGATGTG TTGCTCTTTG CAGCCTTGGT ATTTGGATCT GTGAGGAGCT AGTTCATGAA 
TCTCATCATC CTCAGATCAA GGAAGCATTG AATGTGATTT GTGTTTCTTT AAAGTTTCCG 
AATAAGACAG TAGCTCAAGT TGCCTGCAGT ATGCTGAATA TGCTGATACA TTATGTGCAT 
AGACTTCAAG TGTATCAAGC TGATTCACCT TTAAGAATTA TTCAAATTCT GATAGCTACA 
ATTACCCATC TGCTACCCGG TACAGAAGCT TCATCCTATG AACAGGACAA GAGGTTGGTA 
GTTTCCTTAC TTCTGTGCTT ATTGGATTGG ATAATGGCCT TGCCATTGAA GACCTTGCTG 
CAGCCTCTTC ACGCTACAGG AGCAGAAAAT GATAAGACTG AGAGATCTGT TCTTAATTGT 
ATATATAAGG TTCTACATGG ATGTGTCTAT GGATCTCAGT GTTTTAGTAA CCCCAAATAT 
TTTCCCCTAA GTCTTTCTGA TTTGGCATGT GTGGATTACG ATCCATTTAT GCATTTAGAA 
AGCTTGAAGG AGCCAGAACC ACTGCATTCT CCAGACTCAG AGCGTTCTTC TAAACTTCAA 
CCAGTCACCG AAGTGAAAAC TCACATGCAA CAAGGATTAA TTTCTGTTGC TGCTCGTACT 
GTGATAACGC ATCTAGTCAA CCACTTAGGT CACTATCCTA TGAGTGGAGG ACCTGCTATG 
CTGACTAGTC AAGTGTGTGA AAATAATGAC AATCCATATA GTGAAAGTCC TGAACTTTCT 
CCTGAACTTT TTGAGAACCC AAACCTTCAA TTCTTTGTGT TAAACAATAC TTCCTTGGTG 
TCTTGCATAC AAATCCAAGC AGAAGATGAC ATGCCTGGGG GAGGACTGTC TGCTGGACTT 
GCATCTGCTA ATTCAAATGT CAGAATTATA GTGCGTGATC TGTCTGGCAA ATACTCATGG 
GATTCAGCCA TTCTGTATGG ACCATCATCC TTATGTGCAT CTTCACAACA AACATCTTTT 
GCCCTTTCAT TATCTCAACA AGAAAAAACG GAAGATCCCC TTTCTTCTTT TGAGCATGTG 
GAGGACATAT CTGCAAGGGA TGGAATTACA CTCCAGGTAA AAAGGAAATT CAGAGACACT 
GTGCCAACAT GGGATACAAT TAGGGATGAA GAAGATGCCC TTGATGAGCT CTTGCAGTAT 
TTAGGTGTTA CAAGCCCTGA ATGCTTACAG AGAACTGGAG TCTCACTCAA CATCCCTGCT 
CCTCAACCAG TCTGCATCTC AGAGAAGCAG GAGAATGACG TCATCAATGC AATTCTGAAA 
CAGCATACAG AAGAGAGAGA GTTTGTTGAA AAACATTTCA ATGATTTAAA TATGAGGGCT 
ATGGAGCAGG ATGAGCCAAC ATCGCAAAAG CCCCAGTCAG CATTTTACTA CTGCAGACTA 
CTTCTCAGTA TACTGGGAAT GAATTCTTGG GATAAAAGAA GGAGCTTTCA TCTCCTGAAG 
AAAAATGAAA AGTTACTTCG AGAGCTCAGA AATTTGGACT CAAGACAGTG CCGAGAGACC 
CACAAGATTG CAGTGTTTTA TGTTGCTGAG GGACAAGAAG ATAAACACTC CATTCTTACT 
AATACTGGGG GAAGTCAAGC CTATGAGGAT TTTGTAGCTG GCCTTGGCTG GGAGGTAAAT 
CTCACAAACC ATTGTGGCTT TATGGGAGGA CTGCAAAAAA ACAAAAGCAC TGGATTGACC 
ACTCCATATT TTGCTACCTC AACAGTAGAA GTAATGTTCC ATGTGTCAAC AAGAATGCCT 
TCTGATTCAG ATGACTCTCT AACAAAAAAG CTAAGACATT TAGGGAACGA TGAAGTACAC 
ATTGTTTGGT CAGAGCATAC TCGAGATTAT AGAAGAGGAA TAATTCCAAC CGAATTTGGT 
GATGTTCTTA TTGTTATCTA TCCCATGAAA AACCACATGT TCAGTATCCA GATCATGAAG 
AAGCCTGAGG TTCCATTTTT TGGTCCACTT TTTGATGGTG CTATTGTGAA TGGAAAAATT 
CTTCCTATTA TGGTTCGCGC AACAGCTATA AATGCAAGCC GTGCATTGAA ATCATTAATC 
CCTTTATACC AAAACTTCTA TGAGGAGAGA GCACGATACC TGCAAACAAT TGTTCAGCAT 
CACCTAGAAC CTACAACTTT TGAGGATTTT GCTGCTCAGG TTTTCTGTCC AGCACCTTAC 
CACCATTTGC CCTCAGATGC AGTTGGCTCC TGCCCAGAGA CTCAGCCGGG AGAGCCTCCA 
GCAGCAGCAG CAGCAGCAGC GCAGGCGGAT GGAACCGACT TAGCCTCACC CATGTCTCCT 
CGGACTAGCA AGAGCCGCAT GTCCATGAAG CTGCGGCGCT CCTCGGGCTC GGCCAACAAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( NM_001277451.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264380.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 2, mRNA.

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CloneID	OGa23873
Clone ID Related Accession (Same CDS sequence)	NM_001277451.2 , NM_001277451.1
Accession Version	NM_001277451.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000303.1. On Dec 20, 2018 this sequence version replaced NM_001277451.1. Transcript Variant: This variant (2) lacks an in-frame coding exon in the central region and a 3 nt exon in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment and an internal aa Val, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC AGCAGCGCAG 
GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAGCAAGAG CCGCATGTCC 
ATGAAGCTGC GGCGCTCCTC GGGCTCGGCC AACAAGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264380.1
CDS	217..6618
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	NM_001277451.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277451.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC AGCAGCGCAG 
GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAGCAAGAG CCGCATGTCC 
ATGAAGCTGC GGCGCTCCTC GGGCTCGGCC AACAAGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( NM_001277451.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264380.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 2, mRNA.

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CloneID	OGa23873
Clone ID Related Accession (Same CDS sequence)	NM_001277451.2 , NM_001277451.1
Accession Version	NM_001277451.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-05
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04000014.1. Transcript Variant: This variant (2) lacks an in-frame coding exon in the central region and a 3 nt exon in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment and an internal aa Val, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC AGCAGCGCAG 
GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAGCAAGAG CCGCATGTCC 
ATGAAGCTGC GGCGCTCCTC GGGCTCGGCC AACAAGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264380.1
CDS	217..6618
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	NM_001277451.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277451.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGC TAATAGAGAG 
GACATGAGTC AAAAGCTGCA CCCTATTGAT AGTGATATTG GCAGTAGCAA TACAAATGTT 
CCTGACCTCA TGGATGAATT TATAGCTGAG AGACTCCGCA GTGGTAGTAC ACTGAATGTG 
ACAAGACGGG GAAGCAGTCC TGGCAGCCTG GAAGTTCCAA AAGATCTTCC TGATGTATTG 
AACAAACAGA ACCAAATGCG TCCTATAGAT GACCCAGGCG TGCCTTCCGA ATGGACATCA 
CCTGCTAGCG CAGGCAGCAG TGACCTCATC AGTTCAGATA GCCACTCGGA CTCGTTCAGT 
GCTTTTCAGT ATGATGGACG CAAATTTGAA AATTTCAGCT TTGGCACAGA GGCAGGGACG 
CCAGCTTCCA CTGATGCTGA TGCAATTTCA GGACAGCAGC AGAGTGCTGA GGAACAAGAA 
GTAGCCAGTC TAACTACACT CCACATAGAT TCGGAAACAA GTAGTCTGAA TCAGCAACCG 
TTGTCTGCTG AAGCTGCAAC TGTTACTGGC TCTGAAAGTG CTTCTCCAAT TCAATCTGCT 
CTTGGATCCC GATCACAGAC ACCCTCTCCT GCCACTCTTC ATGCAGATCA TATTGAGCAG 
AAGGATCTGC AACTGGATGA GAAGCTCCAC CACTCTGTTT TGCAGACGCC AGATGACCTA 
GAAACTGGTG AATTTCCCTC AGAATGTTGC AGTGTTATGG CTGGGGGAAC ACTTACAGGA 
TGGCACGCAG ATGTTGCTAC TGTGATGTGG AGACGAATGC TAGGAATTTT AGGTGATGTC 
AACAGCATCA TGGATCCAGA GATTCATGCC CAGGTTTTTG ATTACCTGTG TGAACTGTGG 
CAGAATCTGG CCAAGATAAG AGACAATCTT GGTATTTCAA CAGATAACCT CACTTCACCA 
TCTCCACCAG TTTTAATCCC ACCACTGAGA ATTCTAACAC CTTGGCTGTT CAAGGCAACA 
ATGCTGACTG ATAAATACAA ACAAGGGAAG CTACATGCTT ATAAACTCAT TTGTAAGACA 
ATGAAGCGAA GACAAGATGT ATCTCCAAAC AAGGATTTTT TGACTCATTT CTATAATATA 
ATGCACTGTG GACTTCTTCA TGTTGATCAG GATATTGTCA ATACAATCAT CAAGCACTGC 
TCTCCTCAGT TCTTTTCACT TAGTTTGCCT GGTGCCACCA TGCTTATTAT GGATTTCATT 
GTAGCAGCAG GAAGGGTGGC AGCTTCTTCT TTTCTCAATG CACCAAGAGT TGAAGCTCAA 
GTTCTTTTAG GATCTCTTGT CTGTTTTCCC AATTTATATC ATGAACTACC AGCTCTTCAC 
CCAAACATTC CTGACATAGC TGTGTCTCAG TTTACAGATG TTAAGGAGCT CATAATTAAA 
ACTGTGTTAA GTTCAGCCAG AGAGGAGCCA TCTGGTCCTG CAAGATGTGT TGCTCTTTGC 
AGCCTTGGTA TTTGGATCTG TGAGGAGCTA GTTCATGAAT CTCATCATCC TCAGATCAAG 
GAAGCATTGA ATGTGATTTG TGTTTCTTTA AAGTTTCCGA ATAAGACAGT AGCTCAAGTT 
GCCTGCAGTA TGCTGAATAT GCTGATACAT TATGTGCATA GACTTCAAGT GTATCAAGCT 
GATTCACCTT TAAGAATTAT TCAAATTCTG ATAGCTACAA TTACCCATCT GCTACCCGGT 
ACAGAAGCTT CATCCTATGA ACAGGACAAG AGGTTGGTAG TTTCCTTACT TCTGTGCTTA 
TTGGATTGGA TAATGGCCTT GCCATTGAAG ACCTTGCTGC AGCCTCTTCA CGCTACAGGA 
GCAGAAAATG ATAAGACTGA GAGATCTGTT CTTAATTGTA TATATAAGGT TCTACATGGA 
TGTGTCTATG GATCTCAGTG TTTTAGTAAC CCCAAATATT TTCCCCTAAG TCTTTCTGAT 
TTGGCATGTG TGGATTACGA TCCATTTATG CATTTAGAAA GCTTGAAGGA GCCAGAACCA 
CTGCATTCTC CAGACTCAGA GCGTTCTTCT AAACTTCAAC CAGTCACCGA AGTGAAAACT 
CACATGCAAC AAGGATTAAT TTCTGTTGCT GCTCGTACTG TGATAACGCA TCTAGTCAAC 
CACTTAGGTC ACTATCCTAT GAGTGGAGGA CCTGCTATGC TGACTAGTCA AGTGTGTGAA 
AATAATGACA ATCCATATAG TGAAAGTCCT GAACTTTCTC CTGAACTTTT TGAGAACCCA 
AACCTTCAAT TCTTTGTGTT AAACAATACT TCCTTGGTGT CTTGCATACA AATCCAAGCA 
GAAGATGACA TGCCTGGGGG AGGACTGTCT GCTGGACTTG CATCTGCTAA TTCAAATGTC 
AGAATTATAG TGCGTGATCT GTCTGGCAAA TACTCATGGG ATTCAGCCAT TCTGTATGGA 
CCATCATCCT TATGTGCATC TTCACAACAA ACATCTTTTG CCCTTTCATT ATCTCAACAA 
GAAAAAACGG AAGATCCCCT TTCTTCTTTT GAGCATGTGG AGGACATATC TGCAAGGGAT 
GGAATTACAC TCCAGGTAAA AAGGAAATTC AGAGACACTG TGCCAACATG GGATACAATT 
AGGGATGAAG AAGATGCCCT TGATGAGCTC TTGCAGTATT TAGGTGTTAC AAGCCCTGAA 
TGCTTACAGA GAACTGGAGT CTCACTCAAC ATCCCTGCTC CTCAACCAGT CTGCATCTCA 
GAGAAGCAGG AGAATGACGT CATCAATGCA ATTCTGAAAC AGCATACAGA AGAGAGAGAG 
TTTGTTGAAA AACATTTCAA TGATTTAAAT ATGAGGGCTA TGGAGCAGGA TGAGCCAACA 
TCGCAAAAGC CCCAGTCAGC ATTTTACTAC TGCAGACTAC TTCTCAGTAT ACTGGGAATG 
AATTCTTGGG ATAAAAGAAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GTTACTTCGA 
GAGCTCAGAA ATTTGGACTC AAGACAGTGC CGAGAGACCC ACAAGATTGC AGTGTTTTAT 
GTTGCTGAGG GACAAGAAGA TAAACACTCC ATTCTTACTA ATACTGGGGG AAGTCAAGCC 
TATGAGGATT TTGTAGCTGG CCTTGGCTGG GAGGTAAATC TCACAAACCA TTGTGGCTTT 
ATGGGAGGAC TGCAAAAAAA CAAAAGCACT GGATTGACCA CTCCATATTT TGCTACCTCA 
ACAGTAGAAG TAATGTTCCA TGTGTCAACA AGAATGCCTT CTGATTCAGA TGACTCTCTA 
ACAAAAAAGC TAAGACATTT AGGGAACGAT GAAGTACACA TTGTTTGGTC AGAGCATACT 
CGAGATTATA GAAGAGGAAT AATTCCAACC GAATTTGGTG ATGTTCTTAT TGTTATCTAT 
CCCATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAGA AGCCTGAGGT TCCATTTTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAATTC TTCCTATTAT GGTTCGCGCA 
ACAGCTATAA ATGCAAGCCG TGCATTGAAA TCATTAATCC CTTTATACCA AAACTTCTAT 
GAGGAGAGAG CACGATACCT GCAAACAATT GTTCAGCATC ACCTAGAACC TACAACTTTT 
GAGGATTTTG CTGCTCAGGT TTTCTGTCCA GCACCTTACC ACCATTTGCC CTCAGATGCA 
GGCTCCTGCC CAGAGACTCA GCCGGGAGAG CCTCCAGCAG CAGCAGCAGC AGCAGCGCAG 
GCGGATGGAA CCGACTTAGC CTCACCCATG TCTCCTCGGA CTAGCAAGAG CCGCATGTCC 
ATGAAGCTGC GGCGCTCCTC GGGCTCGGCC AACAAGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( NM_001277452.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264381.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 3, mRNA.

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CloneID	OGa23887
Clone ID Related Accession (Same CDS sequence)	NM_001277452.1 , NM_001277452.2
Accession Version	NM_001277452.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-05
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04000014.1. Transcript Variant: This variant (3) lacks two in-frame coding exons in the central region and a 3 nt exon in the 3' coding region, compared to variant 1. The resulting isoform (3) lacks two internal segments and an internal aa Val, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330256.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG GCTCCTGCCC AGAGACTCAG 
CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC CGACTTAGCC 
TCACCCATGT CTCCTCGGAC TAGCAAGAGC CGCATGTCCA TGAAGCTGCG GCGCTCCTCG 
GGCTCGGCCA ACAAGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264381.1
CDS	217..6477
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	NM_001277452.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277452.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG GCTCCTGCCC AGAGACTCAG 
CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC CGACTTAGCC 
TCACCCATGT CTCCTCGGAC TAGCAAGAGC CGCATGTCCA TGAAGCTGCG GCGCTCCTCG 
GGCTCGGCCA ACAAGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( NM_001277452.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264381.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 3, mRNA.

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CloneID	OGa23887
Clone ID Related Accession (Same CDS sequence)	NM_001277452.1 , NM_001277452.2
Accession Version	NM_001277452.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000303.1. On Dec 20, 2018 this sequence version replaced NM_001277452.1. Transcript Variant: This variant (3) lacks two in-frame coding exons in the central region and a 3 nt exon in the 3' coding region, compared to variant 1. The resulting isoform (3) lacks two internal segments and an internal aa Val, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330256.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG GCTCCTGCCC AGAGACTCAG 
CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC CGACTTAGCC 
TCACCCATGT CTCCTCGGAC TAGCAAGAGC CGCATGTCCA TGAAGCTGCG GCGCTCCTCG 
GGCTCGGCCA ACAAGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264381.1
CDS	217..6477
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	NM_001277452.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277452.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG GCTCCTGCCC AGAGACTCAG 
CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC CGACTTAGCC 
TCACCCATGT CTCCTCGGAC TAGCAAGAGC CGCATGTCCA TGAAGCTGCG GCGCTCCTCG 
GGCTCGGCCA ACAAGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( NM_001277453.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264382.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 4, mRNA.

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CloneID	OGa23898
Clone ID Related Accession (Same CDS sequence)	NM_001277453.1 , NM_001277453.2
Accession Version	NM_001277453.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-05
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04000014.1. Transcript Variant: This variant (4) lacks two in-frame coding exons in the central region, and lacks a 3 nt exon but has an alternate exon in the 3' region, compared to variant 1. The resulting isoform (4) lacks two internal segments and has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330255.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG ATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264382.1
CDS	217..6324
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDADH

Target ORF information:

RefSeq Version	NM_001277453.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277453.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG ATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( NM_001277453.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264382.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 4, mRNA.

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CloneID	OGa23898
Clone ID Related Accession (Same CDS sequence)	NM_001277453.1 , NM_001277453.2
Accession Version	NM_001277453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000303.1. On Dec 20, 2018 this sequence version replaced NM_001277453.1. Transcript Variant: This variant (4) lacks two in-frame coding exons in the central region, and lacks a 3 nt exon but has an alternate exon in the 3' region, compared to variant 1. The resulting isoform (4) lacks two internal segments and has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript from the same strain was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF330255.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG ATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264382.1
CDS	217..6324
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFENFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDADH

Target ORF information:

RefSeq Version	NM_001277453.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277453.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG ACATTGATGA TGCTCAAATT 
CTTCCACGTC CACCTCGAGT CAGACATTTT TCACAGAGTG AGGATGCTCC AAGTGAAGTT 
TTTGGTGCAT TAAATGAGGA ACAACCACTG CCACGAAGTA GCAGCACCTC TGACATCCTG 
GAACCATTTG CAGTTGAACG AGCCAAAGCT AATAGAGAGG ACATGAGTCA AAAGCTGCAC 
CCTATTGATA GTGATATTGG CAGTAGCAAT ACAAATGTTC CTGACCTCAT GGATGAATTT 
ATAGCTGAGA GACTCCGCAG TGGTAGTACA CTGAATGTGA CAAGACGGGG AAGCAGTCCT 
GGCAGCCTGG AAGTTCCAAA AGATCTTCCT GATGTATTGA ACAAACAGAA CCAAATGCGT 
CCTATAGATG ACCCAGGCGT GCCTTCCGAA TGGACATCAC CTGCTAGCGC AGGCAGCAGT 
GACCTCATCA GTTCAGATAG CCACTCGGAC TCGTTCAGTG CTTTTCAGTA TGATGGACGC 
AAATTTGAAA ATTTCAGCTT TGGCACAGAG GCAGGGACGC CAGCTTCCAC TGATGCTGAT 
GCAATTTCAG GACAGCAGCA GAGTGCTGAG GAACAAGAAG TAGCCAGTCT AACTACACTC 
CACATAGATT CGGAAACAAG TAGTCTGAAT CAGCAACCGT TGTCTGCTGA AGCTGCAACT 
GTTACTGGCT CTGAAAGTGC TTCTCCAATT CAATCTGCTC TTGGATCCCG ATCACAGACA 
CCCTCTCCTG CCACTCTTCA TGCAGATCAT ATTGAGCAGA AGGATCTGCA ACTGGATGAG 
AAGCTCCACC ACTCTGTTTT GCAGACGCCA GATGACCTAG AAACTGGTGA ATTTCCCTCA 
GAATGTTGCA GTGTTATGGC TGGGGGAACA CTTACAGGAT GGCACGCAGA TGTTGCTACT 
GTGATGTGGA GACGAATGCT AGGAATTTTA GGTGATGTCA ACAGCATCAT GGATCCAGAG 
ATTCATGCCC AGGTTTTTGA TTACCTGTGT GAACTGTGGC AGAATCTGGC CAAGATAAGA 
GACAATCTTG GTATTTCAAC AGATAACCTC ACTTCACCAT CTCCACCAGT TTTAATCCCA 
CCACTGAGAA TTCTAACACC TTGGCTGTTC AAGGCAACAA TGCTGACTGA TAAATACAAA 
CAAGGGAAGC TACATGCTTA TAAACTCATT TGTAAGACAA TGAAGCGAAG ACAAGATGTA 
TCTCCAAACA AGGATTTTTT GACTCATTTC TATAATATAA TGCACTGTGG ACTTCTTCAT 
GTTGATCAGG ATATTGTCAA TACAATCATC AAGCACTGCT CTCCTCAGTT CTTTTCACTT 
AGTTTGCCTG GTGCCACCAT GCTTATTATG GATTTCATTG TAGCAGCAGG AAGGGTGGCA 
GCTTCTTCTT TTCTCAATGC ACCAAGAGTT GAAGCTCAAG TTCTTTTAGG ATCTCTTGTC 
TGTTTTCCCA ATTTATATCA TGAACTACCA GCTCTTCACC CAAACATTCC TGACATAGCT 
GTGTCTCAGT TTACAGATGT TAAGGAGCTC ATAATTAAAA CTGTGTTAAG TTCAGCCAGA 
GAGGAGCCAT CTGGTCCTGC AAGATGTGTT GCTCTTTGCA GCCTTGGTAT TTGGATCTGT 
GAGGAGCTAG TTCATGAATC TCATCATCCT CAGATCAAGG AAGCATTGAA TGTGATTTGT 
GTTTCTTTAA AGTTTCCGAA TAAGACAGTA GCTCAAGTTG CCTGCAGTAT GCTGAATATG 
CTGATACATT ATGTGCATAG ACTTCAAGTG TATCAAGCTG ATTCACCTTT AAGAATTATT 
CAAATTCTGA TAGCTACAAT TACCCATCTG CTACCCGGTA CAGAAGCTTC ATCCTATGAA 
CAGGACAAGA GGTTGGTAGT TTCCTTACTT CTGTGCTTAT TGGATTGGAT AATGGCCTTG 
CCATTGAAGA CCTTGCTGCA GCCTCTTCAC GCTACAGGAG CAGAAAATGA TAAGACTGAG 
AGATCTGTTC TTAATTGTAT ATATAAGGTT CTACATGGAT GTGTCTATGG ATCTCAGTGT 
TTTAGTAACC CCAAATATTT TCCCCTAAGT CTTTCTGATT TGGCATGTGT GGATTACGAT 
CCATTTATGC ATTTAGAAAG CTTGAAGGAG CCAGAACCAC TGCATTCTCC AGACTCAGAG 
CGTTCTTCTA AACTTCAACC AGTCACCGAA GTGAAAACTC ACATGCAACA AGGATTAATT 
TCTGTTGCTG CTCGTACTGT GATAACGCAT CTAGTCAACC ACTTAGGTCA CTATCCTATG 
AGTGGAGGAC CTGCTATGCT GACTAGTCAA GTGTGTGAAA ATAATGACAA TCCATATAGT 
GAAAGTCCTG AACTTTCTCC TGAACTTTTT GAGAACCCAA ACCTTCAATT CTTTGTGTTA 
AACAATACTT CCTTGGTGTC TTGCATACAA ATCCAAGCAG AAGATGACAT GCCTGGGGGA 
GGACTGTCTG CTGGACTTGC ATCTGCTAAT TCAAATGTCA GAATTATAGT GCGTGATCTG 
TCTGGCAAAT ACTCATGGGA TTCAGCCATT CTGTATGGAC CATCATCCTT ATGTGCATCT 
TCACAACAAA CATCTTTTGC CCTTTCATTA TCTCAACAAG AAAAAACGGA AGATCCCCTT 
TCTTCTTTTG AGCATGTGGA GGACATATCT GCAAGGGATG GAATTACACT CCAGGTAAAA 
AGGAAATTCA GAGACACTGT GCCAACATGG GATACAATTA GGGATGAAGA AGATGCCCTT 
GATGAGCTCT TGCAGTATTT AGGTGTTACA AGCCCTGAAT GCTTACAGAG AACTGGAGTC 
TCACTCAACA TCCCTGCTCC TCAACCAGTC TGCATCTCAG AGAAGCAGGA GAATGACGTC 
ATCAATGCAA TTCTGAAACA GCATACAGAA GAGAGAGAGT TTGTTGAAAA ACATTTCAAT 
GATTTAAATA TGAGGGCTAT GGAGCAGGAT GAGCCAACAT CGCAAAAGCC CCAGTCAGCA 
TTTTACTACT GCAGACTACT TCTCAGTATA CTGGGAATGA ATTCTTGGGA TAAAAGAAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG TTACTTCGAG AGCTCAGAAA TTTGGACTCA 
AGACAGTGCC GAGAGACCCA CAAGATTGCA GTGTTTTATG TTGCTGAGGG ACAAGAAGAT 
AAACACTCCA TTCTTACTAA TACTGGGGGA AGTCAAGCCT ATGAGGATTT TGTAGCTGGC 
CTTGGCTGGG AGGTAAATCT CACAAACCAT TGTGGCTTTA TGGGAGGACT GCAAAAAAAC 
AAAAGCACTG GATTGACCAC TCCATATTTT GCTACCTCAA CAGTAGAAGT AATGTTCCAT 
GTGTCAACAA GAATGCCTTC TGATTCAGAT GACTCTCTAA CAAAAAAGCT AAGACATTTA 
GGGAACGATG AAGTACACAT TGTTTGGTCA GAGCATACTC GAGATTATAG AAGAGGAATA 
ATTCCAACCG AATTTGGTGA TGTTCTTATT GTTATCTATC CCATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAGAA GCCTGAGGTT CCATTTTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAATTCT TCCTATTATG GTTCGCGCAA CAGCTATAAA TGCAAGCCGT 
GCATTGAAAT CATTAATCCC TTTATACCAA AACTTCTATG AGGAGAGAGC ACGATACCTG 
CAAACAATTG TTCAGCATCA CCTAGAACCT ACAACTTTTG AGGATTTTGC TGCTCAGGTT 
TTCTGTCCAG CACCTTACCA CCATTTGCCC TCAGATGCAG ATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287370.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142856.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

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CloneID	OGa36816
Clone ID Related Accession (Same CDS sequence)	XM_015287370.1 , XM_015287370.2
Accession Version	XM_015287370.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGAAA GAAATGCTGT GATATTTCTT 
CTCCTTCCAA AACCTCCACT AGAAAGCATT GGCCACATCA AGGTAAAGGG CAAAGGCTTG 
AAACGGAAGG TCTTTCACGC TAGCTGTGAA TGGCTCCTGC CCAGAGACTC AGCCGGGAGA 
GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA GGCGGATGGA ACCGACTTAG CCTCACCCAT 
GTCTCCTCGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142856.1
CDS	270..8324
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287370.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287370.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGAAA GAAATGCTGT GATATTTCTT 
CTCCTTCCAA AACCTCCACT AGAAAGCATT GGCCACATCA AGGTAAAGGG CAAAGGCTTG 
AAACGGAAGG TCTTTCACGC TAGCTGTGAA TGGCTCCTGC CCAGAGACTC AGCCGGGAGA 
GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA GGCGGATGGA ACCGACTTAG CCTCACCCAT 
GTCTCCTCGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287370.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142856.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.

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CloneID	OGa36816
Clone ID Related Accession (Same CDS sequence)	XM_015287370.1 , XM_015287370.2
Accession Version	XM_015287370.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287370.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGAAA GAAATGCTGT GATATTTCTT 
CTCCTTCCAA AACCTCCACT AGAAAGCATT GGCCACATCA AGGTAAAGGG CAAAGGCTTG 
AAACGGAAGG TCTTTCACGC TAGCTGTGAA TGGCTCCTGC CCAGAGACTC AGCCGGGAGA 
GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA GGCGGATGGA ACCGACTTAG CCTCACCCAT 
GTCTCCTCGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142856.1
CDS	269..8323
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAERNAVIFLLLPKPPLESIGHIKVKGKGLKRKVFHASCEWLLPRDSAGRASSSSSSSSAGGWNRLSLTHVSSD

Target ORF information:

RefSeq Version	XM_015287370.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287370.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGAAA GAAATGCTGT GATATTTCTT 
CTCCTTCCAA AACCTCCACT AGAAAGCATT GGCCACATCA AGGTAAAGGG CAAAGGCTTG 
AAACGGAAGG TCTTTCACGC TAGCTGTGAA TGGCTCCTGC CCAGAGACTC AGCCGGGAGA 
GCCTCCAGCA GCAGCAGCAG CAGCAGCGCA GGCGGATGGA ACCGACTTAG CCTCACCCAT 
GTCTCCTCGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287371.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142857.1 Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.

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CloneID	OGa36817
Clone ID Related Accession (Same CDS sequence)	XM_015287371.2 , XM_015287371.1
Accession Version	XM_015287371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGTTG GCTCCTGCCC AGAGACTCAG 
CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC CGACTTAGCC 
TCACCCATGT CTCCTCGGAC TAGCAAGAGC CGCATGTCCA TGAAGCTGCG GCGCTCCTCG 
GGCTCGGCCA ACAAGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142857.1
CDS	268..8268
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAVGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015287371.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein catalytic alpha subunit 1 (RALGAPA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287371.2

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGTTG GCTCCTGCCC AGAGACTCAG 
CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC CGACTTAGCC 
TCACCCATGT CTCCTCGGAC TAGCAAGAGC CGCATGTCCA TGAAGCTGCG GCGCTCCTCG 
GGCTCGGCCA ACAAGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA1 ( XM_015287371.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142857.1 Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.

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CloneID	OGa36817
Clone ID Related Accession (Same CDS sequence)	XM_015287371.2 , XM_015287371.1
Accession Version	XM_015287371.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ral GTPase-activating protein subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGTTG GCTCCTGCCC AGAGACTCAG 
CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC CGACTTAGCC 
TCACCCATGT CTCCTCGGAC TAGCAAGAGC CGCATGTCCA TGAAGCTGCG GCGCTCCTCG 
GGCTCGGCCA ACAAGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142857.1
CDS	270..8270
Translation	MFSKKPHGDVRKSTQKVLDTRKDPLTRLKHLRVLIENAESIDLKQFFDLHFSHIYYVFFENFVTIEVGLKQKGHKSQREELDSILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSSPQSEYGPRTLDNLINPPLNVQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQEKGFSFLFSNFKKYYLPSIFPNICKETSLYNPILDIPQMRPKPHYVMVKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHAGPHIPGMEGENVPKNIQRAAASMASREDSKNDNTDKQDRNAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRKVFSSKRSNVNFLTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEDKPLFMKEPEEVTQCATVDCDENVVDRNSSEKAKEREEEKAMNSSHVRNSNWARNGCYQETLHKLSEIDTEEQNVRAGMQSVLQVFIINSSNIFFLEPANEIKTLLDEHTDMCKRILNIYRHMVVQVTMDKKTWEQMLLVLLRVTESVLKIPPQTFLQYQGRKNSTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVMSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFPKSSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRPPRVRHFSQSEDAPSEVFGALNEEQPLPRSSSTSDILEPFAVERAKGAVPVIDSSSHHAPSLQSSTETSSMHRSTESHITDSNSRESSLEVGDSIYDHLCHLIGPVEFANTTFEQNQCVDLEGEDDLLSSLREYLKEKEELCGNFEIDECEASAHEGDTSVNRNDRLPLDGLENSDQTGQCTNSITVTERPDNSELHLEQNQSGFISNIAPTQIDIFARNEALDKVRQLNIDKQCESLFDHGPISPSIKEGKRYLYRQAATEADVDSAMEAALAEKHKSAQFNEKITNSRVMHAKKILPKAQVNPQMPHIAGASKLSPTKRSISETVTHRAKIMKITAKKRNSVHVTFRPSTESVQFYNPLENKEAAWKARLRKLSGFSGSGSGNSSSSTSTSSSAATELVRPGIYRPLDAINAASLGSKQSKEITEIQAAVLQKESVAVNQFHNRSAFRSSGQESAQQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPAEAPLRDLYSHVMGYFGRKAAANREDMSQKLHPIDSDIGSSNTNVPDLMDEFIAERLRSGSTLNVTRRGSSPGSLEVPKDLPDVLNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFESTLTATKQNTGHAIGMSSPEVQSQFCDRNENVHNSQLLSAFTSYVNCATVCWKHRRCTYKFYMEDFGDFSFGTEAGTPASTDADAISGQQQSAEEQEVASLTTLHIDSETSSLNQQPLSAEAATVTGSESASPIQSALGSRSQTPSPATLHADHIEQKDLQLDEKLHHSVLQTPDDLETGEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICKTMKRRQDVSPNKDFLTHFYNIMHCGLLHVDQDIVNTIIKHCSPQFFSLSLPGATMLIMDFIVAAGRVAASSFLNAPRVEAQVLLGSLVCFPNLYHELPALHPNIPDIAVSQFTDVKELIIKTVLSSAREEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFPNKTVAQVACSMLNMLIHYVHRLQVYQADSPLRIIQILIATITHLLPGTEASSYEQDKRLVVSLLLCLLDWIMALPLKTLLQPLHATGAENDKTERSVLNCIYKVLHGCVYGSQCFSNPKYFPLSLSDLACVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTHMQQGLISVAARTVITHLVNHLGHYPMSGGPAMLTSQVCENNDNPYSESPELSPELFENPNLQFFVLNNTSLVSCIQIQAEDDMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPSSLCASSQQTSFALSLSQQEKTEDPLSSFEHVEDISARDGITLQVKRKFRDTVPTWDTIRDEEDALDELLQYLGVTSPECLQRTGVSLNIPAPQPVCISEKQENDVINAILKQHTEEREFVEKHFNDLNMRAMEQDEPTSQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVMFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKILPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFCPAPYHHLPSDAVGSCPETQPGEPPAAAAAAAQADGTDLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_015287371.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287371.1

ORF Insert Sequence:

ATGTTCTCCA AGAAGCCGCA CGGGGATGTG CGGAAGTCCA CGCAGAAGGT GCTGGACACC 
CGCAAGGACC CGCTCACCCG CCTCAAGCAT CTGCGCGTCC TCATCGAGAA TGCAGAGTCA 
ATTGATCTCA AACAGTTTTT TGATCTACAT TTCTCACATA TATATTATGT GTTCTTTGAA 
AACTTTGTGA CTATTGAAGT AGGTCTGAAA CAAAAAGGTC ACAAGTCTCA GAGAGAAGAA 
TTAGATTCTA TTCTTTTTAT TTTTGAGAAA ATATTGCAAC TCCTTCCAGA AAGGATTCAT 
CAGAGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AGCTTCTTCA TACTGGAAAT 
TCATTAAAGA TACGTCGAGA GGGTGTGCGT CTCTTTCTGC TGTGGCTGCA AGCACTTCAG 
AATAACTGTA GTAAAGAACA ACTATGGATG TTTTCTTGCT TAATTCCTGG ATTTTCATCA 
CCACAATCCG AATATGGTCC CCGAACACTA GATAATCTCA TTAACCCTCC TCTTAACGTT 
CAAGAAACTC AAGTGACTAT AGAGGAAATC ACTCCACTTG TTCCTCCTCA ATCTGGAGAT 
AAAGGACAAG AAGACTTAAC AAGCTATTTC TTGGAAGCGC TTTTGAAATA CATAGTAATT 
CAGGTTAAGA GTTTAGAATG GAAGAACAAA GAAAACCAGG AAAAGGGATT TTCATTTTTA 
TTCTCAAATT TTAAGAAATA TTATTTACCT TCTATTTTCC CAAACATCTG TAAGGAGACC 
AGCTTATATA ACCCTATACT TGATATACCA CAGATGAGAC CAAAGCCACA CTATGTAATG 
GTTAAGAAAG ATGCTGAAAC CAATGAGGCA ATATATTGCA CAAAGGAACC TTTTATTAAG 
GCTCGTGTCA TTGTCATTCG GTGGTTGGTG TCTTTCTGGC TTGAGCCAAA ACCCCATGCA 
GGACCTCATA TTCCTGGGAT GGAAGGTGAA AATGTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGCATGG CTTCAAGAGA AGACAGCAAA AATGACAACA CTGACAAGCA GGATAGAAAT 
GCAGAGCCAG AACAATCACA TTCTAATACA AGTACTTTAA CAGAGAGAGA ACCAAGTTCT 
TCTAGCCTCT GCAGTATTGA TGAAGAACAT TTAACTGATA TTGAAATTGT CCGCAAAGTC 
TTTTCTTCTA AAAGAAGTAA TGTTAATTTT CTAACTGAGA TTTTTCGACA GGCATTTCTG 
TTGCCAATTT GTGAAGCAGC TGCCATGAGG AAAGTGGTAA AGGTATATCA GGAATGGATT 
CAGCAAGAAG ATAAGCCTTT ATTTATGAAA GAACCTGAAG AAGTAACGCA GTGTGCAACT 
GTAGATTGTG ATGAAAATGT TGTAGACCGC AATTCATCGG AGAAAGCAAA AGAAAGAGAA 
GAGGAAAAAG CTATGAACTC CAGTCATGTT CGGAACTCAA ACTGGGCAAG AAATGGCTGT 
TACCAAGAGA CTTTGCATAA GCTATCTGAG ATTGATACTG AAGAGCAAAA TGTGCGAGCT 
GGAATGCAGT CAGTATTACA GGTTTTTATA ATAAATTCTT CAAACATATT TTTTCTTGAA 
CCTGCAAATG AAATTAAAAC TCTGTTGGAT GAACACACTG ATATGTGTAA ACGGATTCTT 
AATATCTACC GTCACATGGT AGTTCAAGTT ACTATGGACA AGAAGACTTG GGAACAAATG 
CTACTTGTGC TGCTCAGAGT TACTGAATCT GTGCTGAAAA TACCACCCCA AACTTTCTTG 
CAGTATCAAG GAAGAAAAAA CTCCACTTTA GCAGGAAGAC TTGCAGGACC TCTTTTCCAG 
ACTCTTATAG TAGCTTGGAT CAAAGCAAAT CTAAACGTGT ACATCTCTCG AGAACTTTGG 
GATGACTTAC TGTCTGTCAT GTCATCACTG ACATACTGGG AAGAGCTGGC CACTGAGTGG 
TCACTGACAA TGGAGACCCT AACTAAAGTG TTAGCTAGAA ACTTGTATAG TCTGGACCTC 
AGTGACCTGC CATTGGATAA GTTAAGCGAG CAGAAGCAGA AAAAACACAA AGGCAAAGGA 
GTTGGGCATG AATTCCCCAA GTCTTCTGTG GATAAGTCCT TTTCTAGAGG CTGGAGCCGT 
GATCAGCCTG GACAGGCGCC AATGAGACAA CGGAGTGCTA CAACCACTGG ATCTCCAGGA 
ACTGAAAAGG CAAGAAGCAT AGTACGGCAG AAAACTGTTG CCATGAGAAG CCGATCCATT 
GGTGAATGTG CTCTGCCATC GGCCTATATA CGCAGTGCTA AAAGTGCTCC TGTTCTGATC 
CATACTTCCA AACCCTTCTT GCCTGATATT GTTCTCACTC CCCTTTCTGA TGAGCTTTCA 
GACATTGATG ATGCTCAAAT TCTTCCACGT CCACCTCGAG TCAGACATTT TTCACAGAGT 
GAGGATGCTC CAAGTGAAGT TTTTGGTGCA TTAAATGAGG AACAACCACT GCCACGAAGT 
AGCAGCACCT CTGACATCCT GGAACCATTT GCAGTTGAAC GAGCCAAAGG TGCAGTTCCT 
GTCATTGACA GTTCATCCCA TCACGCTCCA AGTTTACAGA GTTCCACTGA GACCTCTTCA 
ATGCATAGAT CCACTGAAAG CCATATTACT GATTCAAATA GCAGAGAGTC CTCCTTGGAA 
GTTGGGGATA GTATTTATGA CCATCTTTGT CATTTAATAG GGCCAGTAGA ATTTGCAAAC 
ACAACCTTTG AACAAAACCA GTGTGTTGAC CTTGAAGGTG AAGATGATCT TCTTTCCTCT 
CTGAGAGAAT ATCTTAAAGA AAAAGAAGAA CTTTGCGGTA ACTTTGAGAT AGATGAATGT 
GAGGCTTCTG CTCACGAAGG TGATACATCA GTAAATAGGA ATGATAGATT ACCACTGGAT 
GGATTAGAAA ATAGTGATCA GACTGGTCAA TGCACGAATA GCATCACTGT TACAGAAAGA 
CCTGATAACT CAGAGCTGCA CCTAGAACAA AACCAAAGTG GCTTTATAAG TAATATTGCA 
CCTACTCAAA TAGACATTTT TGCAAGAAAT GAAGCACTCG ATAAAGTCAG ACAGTTAAAT 
ATCGATAAAC AGTGTGAAAG TCTGTTTGAT CATGGGCCAA TTAGTCCTAG CATCAAAGAA 
GGAAAAAGAT ACCTGTACAG GCAAGCAGCC ACTGAAGCTG ATGTAGATTC AGCCATGGAA 
GCAGCGTTAG CTGAAAAGCA TAAAAGTGCT CAATTTAACG AAAAAATAAC CAACTCACGT 
GTTATGCATG CAAAGAAAAT TCTTCCTAAA GCACAAGTTA ACCCTCAAAT GCCTCACATC 
GCAGGTGCAA GCAAGCTGTC ACCAACTAAA AGGAGTATAT CTGAAACAGT AACTCATAGG 
GCCAAAATCA TGAAAATAAC AGCTAAAAAA AGAAATAGCG TTCATGTAAC TTTTAGACCA 
TCTACAGAAT CAGTTCAGTT TTACAATCCT CTTGAGAACA AAGAGGCTGC TTGGAAAGCA 
AGACTTCGTA AACTTAGTGG CTTCAGCGGT AGTGGTAGCG GCAACAGCAG TAGCAGTACA 
AGCACCAGCT CATCAGCTGC CACGGAGCTG GTTAGACCTG GAATATATAG ACCTCTAGAT 
GCAATCAATG CTGCTTCACT TGGTAGCAAG CAAAGTAAGG AAATTACAGA AATTCAAGCA 
GCTGTGCTGC AAAAGGAAAG TGTTGCAGTT AATCAGTTCC ATAATCGTAG TGCATTTAGA 
TCATCAGGTC AGGAGTCAGC GCAACAGCAG GGCTCCCTGG GTGGTGTTTA TAAAACTGTT 
GTACATGCTC TTTCGAAGCC GAAGGCAAAT GTTTCCCCAC AGAGGCAGAA CAGAATGCCA 
GCAGAGGCTC CACTGAGGGA TCTGTACAGT CATGTAATGG GCTATTTTGG AAGGAAAGCT 
GCAGCTAATA GAGAGGACAT GAGTCAAAAG CTGCACCCTA TTGATAGTGA TATTGGCAGT 
AGCAATACAA ATGTTCCTGA CCTCATGGAT GAATTTATAG CTGAGAGACT CCGCAGTGGT 
AGTACACTGA ATGTGACAAG ACGGGGAAGC AGTCCTGGCA GCCTGGAAGT TCCAAAAGAT 
CTTCCTGATG TATTGAACAA ACAGAACCAA ATGCGTCCTA TAGATGACCC AGGCGTGCCT 
TCCGAATGGA CATCACCTGC TAGCGCAGGC AGCAGTGACC TCATCAGTTC AGATAGCCAC 
TCGGACTCGT TCAGTGCTTT TCAGTATGAT GGACGCAAAT TTGAAAGTAC ACTTACAGCG 
ACTAAGCAGA ATACAGGACA TGCAATAGGA ATGTCTTCTC CAGAAGTTCA GTCGCAGTTT 
TGTGACAGAA ATGAGAATGT CCATAATAGC CAATTACTTT CTGCTTTTAC ATCTTATGTA 
AATTGTGCAA CAGTGTGTTG GAAGCATAGG AGATGTACAT ACAAGTTTTA CATGGAGGAT 
TTTGGAGATT TCAGCTTTGG CACAGAGGCA GGGACGCCAG CTTCCACTGA TGCTGATGCA 
ATTTCAGGAC AGCAGCAGAG TGCTGAGGAA CAAGAAGTAG CCAGTCTAAC TACACTCCAC 
ATAGATTCGG AAACAAGTAG TCTGAATCAG CAACCGTTGT CTGCTGAAGC TGCAACTGTT 
ACTGGCTCTG AAAGTGCTTC TCCAATTCAA TCTGCTCTTG GATCCCGATC ACAGACACCC 
TCTCCTGCCA CTCTTCATGC AGATCATATT GAGCAGAAGG ATCTGCAACT GGATGAGAAG 
CTCCACCACT CTGTTTTGCA GACGCCAGAT GACCTAGAAA CTGGTGAATT TCCCTCAGAA 
TGTTGCAGTG TTATGGCTGG GGGAACACTT ACAGGATGGC ACGCAGATGT TGCTACTGTG 
ATGTGGAGAC GAATGCTAGG AATTTTAGGT GATGTCAACA GCATCATGGA TCCAGAGATT 
CATGCCCAGG TTTTTGATTA CCTGTGTGAA CTGTGGCAGA ATCTGGCCAA GATAAGAGAC 
AATCTTGGTA TTTCAACAGA TAACCTCACT TCACCATCTC CACCAGTTTT AATCCCACCA 
CTGAGAATTC TAACACCTTG GCTGTTCAAG GCAACAATGC TGACTGATAA ATACAAACAA 
GGGAAGCTAC ATGCTTATAA ACTCATTTGT AAGACAATGA AGCGAAGACA AGATGTATCT 
CCAAACAAGG ATTTTTTGAC TCATTTCTAT AATATAATGC ACTGTGGACT TCTTCATGTT 
GATCAGGATA TTGTCAATAC AATCATCAAG CACTGCTCTC CTCAGTTCTT TTCACTTAGT 
TTGCCTGGTG CCACCATGCT TATTATGGAT TTCATTGTAG CAGCAGGAAG GGTGGCAGCT 
TCTTCTTTTC TCAATGCACC AAGAGTTGAA GCTCAAGTTC TTTTAGGATC TCTTGTCTGT 
TTTCCCAATT TATATCATGA ACTACCAGCT CTTCACCCAA ACATTCCTGA CATAGCTGTG 
TCTCAGTTTA CAGATGTTAA GGAGCTCATA ATTAAAACTG TGTTAAGTTC AGCCAGAGAG 
GAGCCATCTG GTCCTGCAAG ATGTGTTGCT CTTTGCAGCC TTGGTATTTG GATCTGTGAG 
GAGCTAGTTC ATGAATCTCA TCATCCTCAG ATCAAGGAAG CATTGAATGT GATTTGTGTT 
TCTTTAAAGT TTCCGAATAA GACAGTAGCT CAAGTTGCCT GCAGTATGCT GAATATGCTG 
ATACATTATG TGCATAGACT TCAAGTGTAT CAAGCTGATT CACCTTTAAG AATTATTCAA 
ATTCTGATAG CTACAATTAC CCATCTGCTA CCCGGTACAG AAGCTTCATC CTATGAACAG 
GACAAGAGGT TGGTAGTTTC CTTACTTCTG TGCTTATTGG ATTGGATAAT GGCCTTGCCA 
TTGAAGACCT TGCTGCAGCC TCTTCACGCT ACAGGAGCAG AAAATGATAA GACTGAGAGA 
TCTGTTCTTA ATTGTATATA TAAGGTTCTA CATGGATGTG TCTATGGATC TCAGTGTTTT 
AGTAACCCCA AATATTTTCC CCTAAGTCTT TCTGATTTGG CATGTGTGGA TTACGATCCA 
TTTATGCATT TAGAAAGCTT GAAGGAGCCA GAACCACTGC ATTCTCCAGA CTCAGAGCGT 
TCTTCTAAAC TTCAACCAGT CACCGAAGTG AAAACTCACA TGCAACAAGG ATTAATTTCT 
GTTGCTGCTC GTACTGTGAT AACGCATCTA GTCAACCACT TAGGTCACTA TCCTATGAGT 
GGAGGACCTG CTATGCTGAC TAGTCAAGTG TGTGAAAATA ATGACAATCC ATATAGTGAA 
AGTCCTGAAC TTTCTCCTGA ACTTTTTGAG AACCCAAACC TTCAATTCTT TGTGTTAAAC 
AATACTTCCT TGGTGTCTTG CATACAAATC CAAGCAGAAG ATGACATGCC TGGGGGAGGA 
CTGTCTGCTG GACTTGCATC TGCTAATTCA AATGTCAGAA TTATAGTGCG TGATCTGTCT 
GGCAAATACT CATGGGATTC AGCCATTCTG TATGGACCAT CATCCTTATG TGCATCTTCA 
CAACAAACAT CTTTTGCCCT TTCATTATCT CAACAAGAAA AAACGGAAGA TCCCCTTTCT 
TCTTTTGAGC ATGTGGAGGA CATATCTGCA AGGGATGGAA TTACACTCCA GGTAAAAAGG 
AAATTCAGAG ACACTGTGCC AACATGGGAT ACAATTAGGG ATGAAGAAGA TGCCCTTGAT 
GAGCTCTTGC AGTATTTAGG TGTTACAAGC CCTGAATGCT TACAGAGAAC TGGAGTCTCA 
CTCAACATCC CTGCTCCTCA ACCAGTCTGC ATCTCAGAGA AGCAGGAGAA TGACGTCATC 
AATGCAATTC TGAAACAGCA TACAGAAGAG AGAGAGTTTG TTGAAAAACA TTTCAATGAT 
TTAAATATGA GGGCTATGGA GCAGGATGAG CCAACATCGC AAAAGCCCCA GTCAGCATTT 
TACTACTGCA GACTACTTCT CAGTATACTG GGAATGAATT CTTGGGATAA AAGAAGGAGC 
TTTCATCTCC TGAAGAAAAA TGAAAAGTTA CTTCGAGAGC TCAGAAATTT GGACTCAAGA 
CAGTGCCGAG AGACCCACAA GATTGCAGTG TTTTATGTTG CTGAGGGACA AGAAGATAAA 
CACTCCATTC TTACTAATAC TGGGGGAAGT CAAGCCTATG AGGATTTTGT AGCTGGCCTT 
GGCTGGGAGG TAAATCTCAC AAACCATTGT GGCTTTATGG GAGGACTGCA AAAAAACAAA 
AGCACTGGAT TGACCACTCC ATATTTTGCT ACCTCAACAG TAGAAGTAAT GTTCCATGTG 
TCAACAAGAA TGCCTTCTGA TTCAGATGAC TCTCTAACAA AAAAGCTAAG ACATTTAGGG 
AACGATGAAG TACACATTGT TTGGTCAGAG CATACTCGAG ATTATAGAAG AGGAATAATT 
CCAACCGAAT TTGGTGATGT TCTTATTGTT ATCTATCCCA TGAAAAACCA CATGTTCAGT 
ATCCAGATCA TGAAGAAGCC TGAGGTTCCA TTTTTTGGTC CACTTTTTGA TGGTGCTATT 
GTGAATGGAA AAATTCTTCC TATTATGGTT CGCGCAACAG CTATAAATGC AAGCCGTGCA 
TTGAAATCAT TAATCCCTTT ATACCAAAAC TTCTATGAGG AGAGAGCACG ATACCTGCAA 
ACAATTGTTC AGCATCACCT AGAACCTACA ACTTTTGAGG ATTTTGCTGC TCAGGTTTTC 
TGTCCAGCAC CTTACCACCA TTTGCCCTCA GATGCAGTTG GCTCCTGCCC AGAGACTCAG 
CCGGGAGAGC CTCCAGCAGC AGCAGCAGCA GCAGCGCAGG CGGATGGAAC CGACTTAGCC 
TCACCCATGT CTCCTCGGAC TAGCAAGAGC CGCATGTCCA TGAAGCTGCG GCGCTCCTCG 
GGCTCGGCCA ACAAGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The GTPase activating Rap/RanGAP domain-like 1 gene is associated with chicken reproductive traits.
PloS one7(4)e33851(2012)
Shen X,Zeng H,Xie L,He J,Li J,Xie X,Luo C,Xu H,Zhou M,Nie Q,Zhang X

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RALGAPA1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

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