Ralgapa1 cDNA ORF clone, Mus musculus(house mouse)

The following Ralgapa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ralgapa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu69687 XM_011244151.1
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu78276 XM_017315142.1
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu48014 XM_006516108.3
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu69686 XM_006516109.2
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu84575 NM_001368814.1
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu48011 XM_006516104.3
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu48013 XM_006516107.3
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu86114 XM_030246825.1
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu86115 XM_030246826.1
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu86116 XM_006516109.3
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu04894 NM_019994.5
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu04894 NM_001112714.2
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu09221 NM_001286263.1
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu10514 NM_001003719.2
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu09221 XM_006516106.2
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu48012 XM_006516105.2
Latest version!
Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu48013 XM_006516107.2 Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu48011 XM_006516104.2 Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu69687
    Clone ID Related Accession (Same CDS sequence) XM_011244151.1
    Accession Version XM_011244151.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011242453.1
    CDS809..4057
    Translation

    Target ORF information:

    RefSeq Version XM_011244151.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011244151.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu78276
    Clone ID Related Accession (Same CDS sequence) XM_017315142.1
    Accession Version XM_017315142.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTTTG TTGGGACTTC TGAAAATACA CCATTTCAAA AAGAACCAAG CTCAGCTGTC 
    TTCACAAGTA CTCTTGCACC TAACCATTCA GATAGCTATC CAAGATCACA AACTGCTGAA
    AAGGCTAAGC AACTAAGTCT TGATGAACAC TCATCGGATC CTAGCTCAGA TAGCCTATGT
    GATAAAGAAA AAAAGAAACA TCTGTGTAGA CAAACAGCCA CAGAAGTAGA TGCCTGTGTA
    GATATGACAG TAGCTGAAAA GCTTAAGAGT GTACAGGTTA ATGAAAAAAT CACGCCATAC
    TTAATGCATG CCAAGAAAAC TGCATTAAAA ACACCGGTAA GCCGCAGAAT GCCTCATGTT
    TCAAACACTA GCAAGCTTTC TCCAACTAAG AGGAGCTTGT CTGAAACAGT AACTCATAGG
    GCCAAAATCA TGAAAATTGC CGCTAAGAAA CGAAATAGTG TTCATGTTAC TTTTAGACCA
    TCTACCGAGT CTGTCCAATT TTATAATCCT CTGGAAAACA AAGAGGCTCC ATGGAAGATG
    AGACTACGGA AGCTCAGTGG CTTTAGCAGT AATAGCAGTA GCACCACCAG CAACACACAG
    ACCAGCTCAA CTAGTGGTAC AGACCTTGTA AAGCCTGGTG TGTACAGACC TCTAGATACA
    ATCGGTGCAG CATCAGTTAG CAGAGCAGTG AGGGAATCCA CAGAGATCCC CACAGCCACA
    TCACAGAAAG AAGGAATGGC AAGTAACCAG GCAGGTAGTC GCAGTACTCT CAGGTCATCG
    AGTCAGGAGA CAGGACCACA GCAGGGCTCC CTGGGTGGCG TTTATAAAAC TGTTGTCCAT
    GCTCTTTCGA AGCCGAAGGC AAATGTTTCC CCACAGAGGC AGAACAGAAT GCCACCCGAG
    GCTCCACTGA GGGATCTGTA CAGTCATGTA ATGGGCTATT TTGGAAGGAA AGCTGCAGTA
    AATAAAGAGG ACACGAGCCC CAAACTGCCT CCTCTTAATA GTGAGACTGG TGGCAACAGC
    GCCAATGTTC CTGACCTGAT GGATGAGTTT ATCGCAGAGC GACTTCGAAG TGGCAATGCC
    TCGACTATGA CAAGAAGGGG GAGTAGTCCA GGCAGCCTTG AAATCCCGAA AGACCTCCCT
    GATATTCTAA ACAAGCAGAA CCAGATGCGG CCTGTTGATG ACCCTGGTGT ACCCTCCGAA
    TGGACGTCTC CAGCCAGTGC AGGGAGCAGT GATCTCATGA GCTCAGATAG TCACTCGGAT
    TCCTTCAGTG CCTTCCAGTG TGAGGGACGA AAATTTGACA ACTTTGGCTT CGGGACTGAC
    ATTGGGATCC CATCTTCTGC TGATGTGGAT TTAGGTTCTG GCCATCATCA AAGCACTGAG
    GAGCAGGAAG TGGCCAGCCT AACTACGCTT CATTTAGATT CAGAAACAAG CAGCCTTAAT
    CAGCAAGCCT TCTCTGCAGA AGTTGCAACC GTTACTGGTT CAGAAAGTGC TTCCCCCGTT
    CATTCAGCTC TGGGATCCAG GTCGCAGACC CCCTCCCCAT CTACACTGAG CAGAGCTCAC
    ATAGAACAGA AGGACCTACA GCTCGATGAG AAGCTCCACC ACTCTGTTCT TCAGACGCCG
    GATGACCTAG AAATCAGTGA ATTTCCATCT GAATGTTGTA GCGTGATGGC AGGAGGTACT
    CTCACTGGGT GGCATGCTGA TGTTGCTACT GTTATGTGGC GCAGAATGCT GGGCATTTTG
    GGAGATGTCA ATGCTATTAT GGATCCTGAA ATACATGCTC AAGTTTTTGA TTACCTCTGT
    GAACTTTGGC AGAATCTAGC CAAGATCAGA GATAACCTTG GCATTTCAGC TGACAACCTG
    ACTTCCCCTT CTCCACCCGT CCTGATTCCT CCACTAAGAA TTCTTACACC TTGGCTTTTT
    AAGGCAACCA TGTTGACTGA TAAGTATAAA CAAGGTAAAT TACATGCATA TAAACTTATT
    TGTAATACAA TGAAGAGAAG ACAAGATGTA TCTCCAAATA GAGATTTTTT AACACATTTC
    TACAATATAA TGCATTGTGG ATTGCTTCAT ATTGACCAGG ACATTGTCAA TACGATCATC
    AAGCACTGCT CACCTCAGTT TTTTTCACTC GGTTTGCCTG GGGCCACGAT GCTTATTATG
    GATTTTATTA TAGCAGCTGG GAGAGTGGCT TCTTCGGCTT TTCTTAATGC ACCAAGAGTG
    GAAGCACAAG TTCTTCTGGG ATCTTTAGTT TGCTTTCCCA ACTTATATTG TGAATTGCCT
    GCTCTCCATC CCAACATTCC TGATATTGCT GTGTCTCAAT TTACAGATGT TAAGGAACTT
    ATAATTAAAA CTGTATTAAG CTCGGCAAGA GATGAGCCCT CTGGTCCTGC ACGATGTGTA
    GCACTTTGCA GTTTAGGTAT TTGGATTTGT GAAGAATTAG TCCACGAATC TCATCATCCT
    CAAATTAAAG AAGCACTGAA TGTAATTTGT GTTTCATTAA AGTTTACTAA TAAAACAGTT
    GCCCACGTCG CCTGTAACAT GCTTCACATG CTGGTTCACT ATGTCCCTAG ACTTCAGATT
    CACCAGCCGC AGTCTCCCCT GAAAATTATT CAGATCCTAA TAGCAACTAT CACCCACCTT
    TTGCCAAGTA CAGAAGCTTC ATCATATGAA ATGGACAAGA GGTTGGTTGT CTCATTACTT
    CTTTGCCTTC TGGACTGGAT CATGGCCTTA CCTTTAAAGA CACTGCTCCA ACCAGTCCAT
    GCTACAGGAG CAGAAAATGA TAAGACCGAA AAGTCTGTTC TCAACTGCAT TTATAAGGTA
    TTACATGGGT GTGTCTATGG AGCGCAAAGT TTTAGCAATC CCAAGTACTT TCCAATAAGC
    CTTTCTGATT TGGCATCTGT AGATTATGAT CCGTTTATGC ATTTGGAAAG TCTGAAAGAA
    CCTGAACCTT TACACTCTCC AGATTCAGAG CGGTCTTCTA AACTGCAGCC TGTGACAGAA
    GTGAAAACTC AGATGCAACA AGGATTAATC TCTATAGCAG CCCGTACTGT TATTACCCAT
    CTGGTGAATC ACTTGGGCCA TTATCCAATG AGTGGTGGTC CTGCTATGCT AACAAGTCAG
    GTGTGTGAAA ATCATGACAA TCATTACAGT GAAAGTACTG AACTTTCTCC TGAGCTCTTT
    GAAAGTCCAA ACATCCAGTT CTTTGTGCTG AATAATACAA CCTTAGTGTC CTGTATCCAG
    ATAAGATCGG AAGAGAGTAT GCCTGGAGGA GGTCTAGCTG CTGGCCTTGT GTCAGCCAAC
    TCAAATGTTA GAATCATAGT ACGTGATCTC TCTGGAAAGT ACTCATGGGA TTCTGCCATA
    CTGTATGGAC CACCCATTGT AAGTGGTTTG CCTGAACCTA CATCTTTCAT ACTTTCAATG
    TCTGACCAAG AGAAACCGGA AGAGCCTCCT ACATCTAACG AATGCTTAGA AGACATCGCA
    GTAAAAGATG GGCTTTCTCT CCAGCTTAGA AGATTTAGAG AAACTGTACC AACTTGGTCT
    ACGATACGAG AGGAAGAAGA TGTTCTTGAC GAGCTCCTGC AGTATTTAGG CACTACAAGT
    CCTGAGTGTT TACAGAGAAC GGGAATCTCC CTCAATGTTC CTGCTCCACA GCCTCTGTGC
    ATTTCTGAAA AACAGGAGAA TGATGTCATT AATGCTATCC TTAAGCAATA CACGGAAGAA
    AAAGAGTTTG TAGAGAAGCA CTTTAATGAC TTAAACATGA AAGCCTCAGA ACAAGATGAG
    CCAACACCTC AGAAACCTCA GTCAGCTTTT TATTACTGCA GATTGCTTCT TAGTATATTG
    GGAATGAATT CCTGGGACAA ACGGAGGAGC TTTCATCTCT TGAAGAAAAA TGAAAAGCTG
    CTCAGAGAAC TTAGGAACTT GGATTCAAGG CAATGCCGAG AGACACACAA GATTGCAGTA
    TTTTACGTTG CTGAAGGACA AGAAGATAAA TACTCCATTC TCACCAATAT AGGAGGAAGC
    CAAGCATATG AAGATTTTGT AGCTGGCCTT GGTTGGGAGG TAAACCTTAC AAACCACTGT
    GGCTTCATGG GAGGCCTTCA GAAGAACAGA AGCACTGGAC TGACCACTCC CTATTTTGCT
    ACCTCGACAG TAGAAGTAAT ATTTCATGTA TCTACACGAA TGCCTTCTGA TTCTGATGAC
    TCTCTGACCA AAAAATTGAG ACATCTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAA
    CATACTAGAG ACTACAGGAG AGGAATTATT CCTACTGAGT TTGGTGATGT CCTCATTGTA
    ATATACCCAA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAAAAACC AGAGGTTCCC
    TTCTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAGTTCTGCC CATTATGGTT
    CGATCCACAG CAATAAATGC TAGCCGTGCT CTGAAATCAC TGATTCCATT GTATCAAAAT
    TTCTATGAGG AGAGAGCACG GTACCTGCAG ACAATTGTCC AGCACCATTT AGAGCCAACA
    ACATTTGAAG ACTTTGCAGC ACAGGTTTTC TCTCCAGCTC CCTACCATCA TTTCCCTGCT
    GACGCAGATC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170631.1
    CDS405..5039
    Translation

    Target ORF information:

    RefSeq Version XM_017315142.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTTTG TTGGGACTTC TGAAAATACA CCATTTCAAA AAGAACCAAG CTCAGCTGTC 
    TTCACAAGTA CTCTTGCACC TAACCATTCA GATAGCTATC CAAGATCACA AACTGCTGAA
    AAGGCTAAGC AACTAAGTCT TGATGAACAC TCATCGGATC CTAGCTCAGA TAGCCTATGT
    GATAAAGAAA AAAAGAAACA TCTGTGTAGA CAAACAGCCA CAGAAGTAGA TGCCTGTGTA
    GATATGACAG TAGCTGAAAA GCTTAAGAGT GTACAGGTTA ATGAAAAAAT CACGCCATAC
    TTAATGCATG CCAAGAAAAC TGCATTAAAA ACACCGGTAA GCCGCAGAAT GCCTCATGTT
    TCAAACACTA GCAAGCTTTC TCCAACTAAG AGGAGCTTGT CTGAAACAGT AACTCATAGG
    GCCAAAATCA TGAAAATTGC CGCTAAGAAA CGAAATAGTG TTCATGTTAC TTTTAGACCA
    TCTACCGAGT CTGTCCAATT TTATAATCCT CTGGAAAACA AAGAGGCTCC ATGGAAGATG
    AGACTACGGA AGCTCAGTGG CTTTAGCAGT AATAGCAGTA GCACCACCAG CAACACACAG
    ACCAGCTCAA CTAGTGGTAC AGACCTTGTA AAGCCTGGTG TGTACAGACC TCTAGATACA
    ATCGGTGCAG CATCAGTTAG CAGAGCAGTG AGGGAATCCA CAGAGATCCC CACAGCCACA
    TCACAGAAAG AAGGAATGGC AAGTAACCAG GCAGGTAGTC GCAGTACTCT CAGGTCATCG
    AGTCAGGAGA CAGGACCACA GCAGGGCTCC CTGGGTGGCG TTTATAAAAC TGTTGTCCAT
    GCTCTTTCGA AGCCGAAGGC AAATGTTTCC CCACAGAGGC AGAACAGAAT GCCACCCGAG
    GCTCCACTGA GGGATCTGTA CAGTCATGTA ATGGGCTATT TTGGAAGGAA AGCTGCAGTA
    AATAAAGAGG ACACGAGCCC CAAACTGCCT CCTCTTAATA GTGAGACTGG TGGCAACAGC
    GCCAATGTTC CTGACCTGAT GGATGAGTTT ATCGCAGAGC GACTTCGAAG TGGCAATGCC
    TCGACTATGA CAAGAAGGGG GAGTAGTCCA GGCAGCCTTG AAATCCCGAA AGACCTCCCT
    GATATTCTAA ACAAGCAGAA CCAGATGCGG CCTGTTGATG ACCCTGGTGT ACCCTCCGAA
    TGGACGTCTC CAGCCAGTGC AGGGAGCAGT GATCTCATGA GCTCAGATAG TCACTCGGAT
    TCCTTCAGTG CCTTCCAGTG TGAGGGACGA AAATTTGACA ACTTTGGCTT CGGGACTGAC
    ATTGGGATCC CATCTTCTGC TGATGTGGAT TTAGGTTCTG GCCATCATCA AAGCACTGAG
    GAGCAGGAAG TGGCCAGCCT AACTACGCTT CATTTAGATT CAGAAACAAG CAGCCTTAAT
    CAGCAAGCCT TCTCTGCAGA AGTTGCAACC GTTACTGGTT CAGAAAGTGC TTCCCCCGTT
    CATTCAGCTC TGGGATCCAG GTCGCAGACC CCCTCCCCAT CTACACTGAG CAGAGCTCAC
    ATAGAACAGA AGGACCTACA GCTCGATGAG AAGCTCCACC ACTCTGTTCT TCAGACGCCG
    GATGACCTAG AAATCAGTGA ATTTCCATCT GAATGTTGTA GCGTGATGGC AGGAGGTACT
    CTCACTGGGT GGCATGCTGA TGTTGCTACT GTTATGTGGC GCAGAATGCT GGGCATTTTG
    GGAGATGTCA ATGCTATTAT GGATCCTGAA ATACATGCTC AAGTTTTTGA TTACCTCTGT
    GAACTTTGGC AGAATCTAGC CAAGATCAGA GATAACCTTG GCATTTCAGC TGACAACCTG
    ACTTCCCCTT CTCCACCCGT CCTGATTCCT CCACTAAGAA TTCTTACACC TTGGCTTTTT
    AAGGCAACCA TGTTGACTGA TAAGTATAAA CAAGGTAAAT TACATGCATA TAAACTTATT
    TGTAATACAA TGAAGAGAAG ACAAGATGTA TCTCCAAATA GAGATTTTTT AACACATTTC
    TACAATATAA TGCATTGTGG ATTGCTTCAT ATTGACCAGG ACATTGTCAA TACGATCATC
    AAGCACTGCT CACCTCAGTT TTTTTCACTC GGTTTGCCTG GGGCCACGAT GCTTATTATG
    GATTTTATTA TAGCAGCTGG GAGAGTGGCT TCTTCGGCTT TTCTTAATGC ACCAAGAGTG
    GAAGCACAAG TTCTTCTGGG ATCTTTAGTT TGCTTTCCCA ACTTATATTG TGAATTGCCT
    GCTCTCCATC CCAACATTCC TGATATTGCT GTGTCTCAAT TTACAGATGT TAAGGAACTT
    ATAATTAAAA CTGTATTAAG CTCGGCAAGA GATGAGCCCT CTGGTCCTGC ACGATGTGTA
    GCACTTTGCA GTTTAGGTAT TTGGATTTGT GAAGAATTAG TCCACGAATC TCATCATCCT
    CAAATTAAAG AAGCACTGAA TGTAATTTGT GTTTCATTAA AGTTTACTAA TAAAACAGTT
    GCCCACGTCG CCTGTAACAT GCTTCACATG CTGGTTCACT ATGTCCCTAG ACTTCAGATT
    CACCAGCCGC AGTCTCCCCT GAAAATTATT CAGATCCTAA TAGCAACTAT CACCCACCTT
    TTGCCAAGTA CAGAAGCTTC ATCATATGAA ATGGACAAGA GGTTGGTTGT CTCATTACTT
    CTTTGCCTTC TGGACTGGAT CATGGCCTTA CCTTTAAAGA CACTGCTCCA ACCAGTCCAT
    GCTACAGGAG CAGAAAATGA TAAGACCGAA AAGTCTGTTC TCAACTGCAT TTATAAGGTA
    TTACATGGGT GTGTCTATGG AGCGCAAAGT TTTAGCAATC CCAAGTACTT TCCAATAAGC
    CTTTCTGATT TGGCATCTGT AGATTATGAT CCGTTTATGC ATTTGGAAAG TCTGAAAGAA
    CCTGAACCTT TACACTCTCC AGATTCAGAG CGGTCTTCTA AACTGCAGCC TGTGACAGAA
    GTGAAAACTC AGATGCAACA AGGATTAATC TCTATAGCAG CCCGTACTGT TATTACCCAT
    CTGGTGAATC ACTTGGGCCA TTATCCAATG AGTGGTGGTC CTGCTATGCT AACAAGTCAG
    GTGTGTGAAA ATCATGACAA TCATTACAGT GAAAGTACTG AACTTTCTCC TGAGCTCTTT
    GAAAGTCCAA ACATCCAGTT CTTTGTGCTG AATAATACAA CCTTAGTGTC CTGTATCCAG
    ATAAGATCGG AAGAGAGTAT GCCTGGAGGA GGTCTAGCTG CTGGCCTTGT GTCAGCCAAC
    TCAAATGTTA GAATCATAGT ACGTGATCTC TCTGGAAAGT ACTCATGGGA TTCTGCCATA
    CTGTATGGAC CACCCATTGT AAGTGGTTTG CCTGAACCTA CATCTTTCAT ACTTTCAATG
    TCTGACCAAG AGAAACCGGA AGAGCCTCCT ACATCTAACG AATGCTTAGA AGACATCGCA
    GTAAAAGATG GGCTTTCTCT CCAGCTTAGA AGATTTAGAG AAACTGTACC AACTTGGTCT
    ACGATACGAG AGGAAGAAGA TGTTCTTGAC GAGCTCCTGC AGTATTTAGG CACTACAAGT
    CCTGAGTGTT TACAGAGAAC GGGAATCTCC CTCAATGTTC CTGCTCCACA GCCTCTGTGC
    ATTTCTGAAA AACAGGAGAA TGATGTCATT AATGCTATCC TTAAGCAATA CACGGAAGAA
    AAAGAGTTTG TAGAGAAGCA CTTTAATGAC TTAAACATGA AAGCCTCAGA ACAAGATGAG
    CCAACACCTC AGAAACCTCA GTCAGCTTTT TATTACTGCA GATTGCTTCT TAGTATATTG
    GGAATGAATT CCTGGGACAA ACGGAGGAGC TTTCATCTCT TGAAGAAAAA TGAAAAGCTG
    CTCAGAGAAC TTAGGAACTT GGATTCAAGG CAATGCCGAG AGACACACAA GATTGCAGTA
    TTTTACGTTG CTGAAGGACA AGAAGATAAA TACTCCATTC TCACCAATAT AGGAGGAAGC
    CAAGCATATG AAGATTTTGT AGCTGGCCTT GGTTGGGAGG TAAACCTTAC AAACCACTGT
    GGCTTCATGG GAGGCCTTCA GAAGAACAGA AGCACTGGAC TGACCACTCC CTATTTTGCT
    ACCTCGACAG TAGAAGTAAT ATTTCATGTA TCTACACGAA TGCCTTCTGA TTCTGATGAC
    TCTCTGACCA AAAAATTGAG ACATCTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAA
    CATACTAGAG ACTACAGGAG AGGAATTATT CCTACTGAGT TTGGTGATGT CCTCATTGTA
    ATATACCCAA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAAAAACC AGAGGTTCCC
    TTCTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAGTTCTGCC CATTATGGTT
    CGATCCACAG CAATAAATGC TAGCCGTGCT CTGAAATCAC TGATTCCATT GTATCAAAAT
    TTCTATGAGG AGAGAGCACG GTACCTGCAG ACAATTGTCC AGCACCATTT AGAGCCAACA
    ACATTTGAAG ACTTTGCAGC ACAGGTTTTC TCTCCAGCTC CCTACCATCA TTTCCCTGCT
    GACGCAGATC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48014
    Clone ID Related Accession (Same CDS sequence) XM_006516108.3
    Accession Version XM_006516108.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516108.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAC GCATCTCAGT CCATCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516171.1
    CDS809..6658
    Translation

    Target ORF information:

    RefSeq Version XM_006516108.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516108.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAC GCATCTCAGT CCATCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu69686
    Clone ID Related Accession (Same CDS sequence) XM_006516109.2
    Accession Version XM_006516109.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006516109.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTTTG TTGGGACTTC TGAAAATACA CCATTTCAAA AAGAACCAAG CTCAGCTGTC 
    TTCACAAGTA CTCTTGCACC TAACCATTCA GATAGCTATC CAAGATCACA AACTGCTGAA
    AAGGCTAAGC AACTAAGTCT TGATGAACAC TCATCGGATC CTAGCTCAGA TAGCCTATGT
    GATAAAGAAA AAAAGAAACA TCTGTGTAGA CAAACAGCCA CAGAAGTAGA TGCCTGTGTA
    GATATGACAG TAGCTGAAAA GCTTAAGAGT GTACAGGTTA ATGAAAAAAT CACGCCATAC
    TTAATGCATG CCAAGAAAAC TGCATTAAAA ACACCGGTAA GCCGCAGAAT GCCTCATGTT
    TCAAACACTA GCAAGCTTTC TCCAACTAAG AGGAGCTTGT CTGAAACAGT AACTCATAGG
    GCCAAAATCA TGAAAATTGC CGCTAAGAAA CGAAATAGTG TTCATGTTAC TTTTAGACCA
    TCTACCGAGT CTGTCCAATT TTATAATCCT CTGGAAAACA AAGAGGCTCC ATGGAAGATG
    AGACTACGGA AGCTCAGTGG CTTTAGCAGT AATAGCAGTA GCACCACCAG CAACACACAG
    ACCAGCTCAA CTAGTGGTAC AGACCTTGTA AAGCCTGGTG TGTACAGACC TCTAGATACA
    ATCGGTGCAG CATCAGTTAG CAGAGCAGTG AGGGAATCCA CAGAGATCCC CACAGCCACA
    TCACAGAAAG AAGGAATGGC AAGTAACCAG GCAGGTAGTC GCAGTACTCT CAGGTCATCG
    AGTCAGGAGA CAGGACCACA GCAGGGCTCC CTGGGTGGCG TTTATAAAAC TGTTGTCCAT
    GCTCTTTCGA AGCCGAAGGC AAATGTTTCC CCACAGAGGC AGAACAGAAT GCCACCCGAG
    GCTCCACTGA GGGATCTGTA CAGTCATGTA ATGGGCTATT TTGGAAGGAA AGCTGCAGTA
    AATAAAGAGG ACACGAGCCC CAAACTGCCT CCTCTTAATA GTGAGACTGG TGGCAACAGC
    GCCAATGTTC CTGACCTGAT GGATGAGTTT ATCGCAGAGC GACTTCGAAG TGGCAATGCC
    TCGACTATGA CAAGAAGGGG GAGTAGTCCA GGCAGCCTTG AAATCCCGAA AGACCTCCCT
    GATATTCTAA ACAAGCAGAA CCAGATGCGG CCTGTTGATG ACCCTGGTGT ACCCTCCGAA
    TGGACGTCTC CAGCCAGTGC AGGGAGCAGT GATCTCATGA GCTCAGATAG TCACTCGGAT
    TCCTTCAGTG CCTTCCAGTG TGAGGGACGA AAATTTGACA ACTTTGGCTT CGGGACTGAC
    ATTGGGATCC CATCTTCTGC TGATGTGGAT TTAGGTTCTG GCCATCATCA AAGCACTGAG
    GAGCAGGAAG TGGCCAGCCT AACTACGCTT CATTTAGATT CAGAAACAAG CAGCCTTAAT
    CAGCAAGCCT TCTCTGCAGA AGTTGCAACC GTTACTGGTT CAGAAAGTGC TTCCCCCGTT
    CATTCAGCTC TGGGATCCAG GTCGCAGACC CCCTCCCCAT CTACACTGAG CAGAGCTCAC
    ATAGAACAGA AGGACCTACA GCTCGATGAG AAGCTCCACC ACTCTGTTCT TCAGACGCCG
    GATGACCTAG AAATCAGTGA ATTTCCATCT GAATGTTGTA GCGTGATGGC AGGAGGTACT
    CTCACTGGGT GGCATGCTGA TGTTGCTACT GTTATGTGGC GCAGAATGCT GGGCATTTTG
    GGAGATGTCA ATGCTATTAT GGATCCTGAA ATACATGCTC AAGTTTTTGA TTACCTCTGT
    GAACTTTGGC AGAATCTAGC CAAGATCAGA GATAACCTTG GCATTTCAGC TGACAACCTG
    ACTTCCCCTT CTCCACCCGT CCTGATTCCT CCACTAAGAA TTCTTACACC TTGGCTTTTT
    AAGGCAACCA TGTTGACTGA TAAGTATAAA CAAGGTAAAT TACATGCATA TAAACTTATT
    TGTAATACAA TGAAGAGAAG ACAAGATGTA TCTCCAAATA GAGATTTTTT AACACATTTC
    TACAATATAA TGCATTGTGG ATTGCTTCAT ATTGACCAGG ACATTGTCAA TACGATCATC
    AAGCACTGCT CACCTCAGTT TTTTTCACTC GGTTTGCCTG GGGCCACGAT GCTTATTATG
    GATTTTATTA TAGCAGCTGG GAGAGTGGCT TCTTCGGCTT TTCTTAATGC ACCAAGAGTG
    GAAGCACAAG TTCTTCTGGG ATCTTTAGTT TGCTTTCCCA ACTTATATTG TGAATTGCCT
    GCTCTCCATC CCAACATTCC TGATATTGCT GTGTCTCAAT TTACAGATGT TAAGGAACTT
    ATAATTAAAA CTGTATTAAG CTCGGCAAGA GATGAGCCCT CTGGTCCTGC ACGATGTGTA
    GCACTTTGCA GTTTAGGTAT TTGGATTTGT GAAGAATTAG TCCACGAATC TCATCATCCT
    CAAATTAAAG AAGCACTGAA TGTAATTTGT GTTTCATTAA AGTTTACTAA TAAAACAGTT
    GCCCACGTCG CCTGTAACAT GCTTCACATG CTGGTTCACT ATGTCCCTAG ACTTCAGATT
    CACCAGCCGC AGTCTCCCCT GAAAATTATT CAGATCCTAA TAGCAACTAT CACCCACCTT
    TTGCCAAGTA CAGAAGCTTC ATCATATGAA ATGGACAAGA GGTTGGTTGT CTCATTACTT
    CTTTGCCTTC TGGACTGGAT CATGGCCTTA CCTTTAAAGA CACTGCTCCA ACCAGTCCAT
    GCTACAGGAG CAGAAAATGA TAAGACCGAA AAGTCTGTTC TCAACTGCAT TTATAAGGTA
    TTACATGGGT GTGTCTATGG AGCGCAAAGT TTTAGCAATC CCAAGTACTT TCCAATAAGC
    CTTTCTGATT TGGCATCTGT AGATTATGAT CCGTTTATGC ATTTGGAAAG TCTGAAAGAA
    CCTGAACCTT TACACTCTCC AGATTCAGAG CGGTCTTCTA AACTGCAGCC TGTGACAGAA
    GTGAAAACTC AGATGCAACA AGGATTAATC TCTATAGCAG CCCGTACTGT TATTACCCAT
    CTGGTGAATC ACTTGGGCCA TTATCCAATG AGTGGTGGTC CTGCTATGCT AACAAGTCAG
    GTGTGTGAAA ATCATGACAA TCATTACAGT GAAAGTACTG AACTTTCTCC TGAGCTCTTT
    GAAAGTCCAA ACATCCAGTT CTTTGTGCTG AATAATACAA CCTTAGTGTC CTGTATCCAG
    ATAAGATCGG AAGAGAGTAT GCCTGGAGGA GGTCTAGCTG CTGGCCTTGT GTCAGCCAAC
    TCAAATGTTA GAATCATAGT ACGTGATCTC TCTGGAAAGT ACTCATGGGA TTCTGCCATA
    CTGTATGGAC CACCCATTGT AAGTGGTTTG CCTGAACCTA CATCTTTCAT ACTTTCAATG
    TCTGACCAAG AGAAACCGGA AGAGCCTCCT ACATCTAACG AATGCTTAGA AGACATCGCA
    GTAAAAGATG GGCTTTCTCT CCAGCTTAGA AGATTTAGAG AAACTGTACC AACTTGGTCT
    ACGATACGAG AGGAAGAAGA TGTTCTTGAC GAGCTCCTGC AGTATTTAGG CACTACAAGT
    CCTGAGTGTT TACAGAGAAC GGGAATCTCC CTCAATGTTC CTGCTCCACA GCCTCTGTGC
    ATTTCTGAAA AACAGGAGAA TGATGTCATT AATGCTATCC TTAAGCAATA CACGGAAGAA
    AAAGAGTTTG TAGAGAAGCA CTTTAATGAC TTAAACATGA AAGCCTCAGA ACAAGATGAG
    CCAACACCTC AGAAACCTCA GTCAGCTTTT TATTACTGCA GATTGCTTCT TAGTATATTG
    GGAATGAATT CCTGGGACAA ACGGAGGAGC TTTCATCTCT TGAAGAAAAA TGAAAAGCTG
    CTCAGAGAAC TTAGGAACTT GGATTCAAGG CAATGCCGAG AGACACACAA GATTGCAGTA
    TTTTACGTTG CTGAAGGACA AGAAGATAAA TACTCCATTC TCACCAATAT AGGAGGAAGC
    CAAGCATATG AAGATTTTGT AGCTGGCCTT GGTTGGGAGG TAAACCTTAC AAACCACTGT
    GGCTTCATGG GAGGCCTTCA GAAGAACAGA AGCACTGGAC TGACCACTCC CTATTTTGCT
    ACCTCGACAG TAGAAGTAAT ATTTCATGTA TCTACACGAA TGCCTTCTGA TTCTGATGAC
    TCTCTGACCA AAAAATTGAG ACATCTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAA
    CATACTAGAG ACTACAGGAG AGGAATTATT CCTACTGAGT TTGGTGATGT CCTCATTGTA
    ATATACCCAA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAAAAACC AGAGGTTCCC
    TTCTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAGTTCTGCC CATTATGGTT
    CGATCCACAG CAATAAATGC TAGCCGTGCT CTGAAATCAC TGATTCCATT GTATCAAAAT
    TTCTATGAGG AGAGAGCACG GTACCTGCAG ACAATTGTCC AGCACCATTT AGAGCCAACA
    ACATTTGAAG ACTTTGCAGC ACAGGTTTTC TCTCCAGCTC CCTACCATCA TTTCCCTGCT
    GACGCAGTTG GATCCTACCC AGAGATTCCA CCCAGTGACG CTGCCCCAGC AGCACAGGTA
    GACGGGGCTG ACCTGGCCTC TCCCATGTCT CCTCGAACTA GCAAAAGCCG CATGTCCATG
    AAGCTGCGTC GGTCCTCTGG CTCAGCCAAT AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516172.2
    CDS405..5183
    Translation

    Target ORF information:

    RefSeq Version XM_006516109.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516109.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCTTTTG TTGGGACTTC TGAAAATACA CCATTTCAAA AAGAACCAAG CTCAGCTGTC 
    TTCACAAGTA CTCTTGCACC TAACCATTCA GATAGCTATC CAAGATCACA AACTGCTGAA
    AAGGCTAAGC AACTAAGTCT TGATGAACAC TCATCGGATC CTAGCTCAGA TAGCCTATGT
    GATAAAGAAA AAAAGAAACA TCTGTGTAGA CAAACAGCCA CAGAAGTAGA TGCCTGTGTA
    GATATGACAG TAGCTGAAAA GCTTAAGAGT GTACAGGTTA ATGAAAAAAT CACGCCATAC
    TTAATGCATG CCAAGAAAAC TGCATTAAAA ACACCGGTAA GCCGCAGAAT GCCTCATGTT
    TCAAACACTA GCAAGCTTTC TCCAACTAAG AGGAGCTTGT CTGAAACAGT AACTCATAGG
    GCCAAAATCA TGAAAATTGC CGCTAAGAAA CGAAATAGTG TTCATGTTAC TTTTAGACCA
    TCTACCGAGT CTGTCCAATT TTATAATCCT CTGGAAAACA AAGAGGCTCC ATGGAAGATG
    AGACTACGGA AGCTCAGTGG CTTTAGCAGT AATAGCAGTA GCACCACCAG CAACACACAG
    ACCAGCTCAA CTAGTGGTAC AGACCTTGTA AAGCCTGGTG TGTACAGACC TCTAGATACA
    ATCGGTGCAG CATCAGTTAG CAGAGCAGTG AGGGAATCCA CAGAGATCCC CACAGCCACA
    TCACAGAAAG AAGGAATGGC AAGTAACCAG GCAGGTAGTC GCAGTACTCT CAGGTCATCG
    AGTCAGGAGA CAGGACCACA GCAGGGCTCC CTGGGTGGCG TTTATAAAAC TGTTGTCCAT
    GCTCTTTCGA AGCCGAAGGC AAATGTTTCC CCACAGAGGC AGAACAGAAT GCCACCCGAG
    GCTCCACTGA GGGATCTGTA CAGTCATGTA ATGGGCTATT TTGGAAGGAA AGCTGCAGTA
    AATAAAGAGG ACACGAGCCC CAAACTGCCT CCTCTTAATA GTGAGACTGG TGGCAACAGC
    GCCAATGTTC CTGACCTGAT GGATGAGTTT ATCGCAGAGC GACTTCGAAG TGGCAATGCC
    TCGACTATGA CAAGAAGGGG GAGTAGTCCA GGCAGCCTTG AAATCCCGAA AGACCTCCCT
    GATATTCTAA ACAAGCAGAA CCAGATGCGG CCTGTTGATG ACCCTGGTGT ACCCTCCGAA
    TGGACGTCTC CAGCCAGTGC AGGGAGCAGT GATCTCATGA GCTCAGATAG TCACTCGGAT
    TCCTTCAGTG CCTTCCAGTG TGAGGGACGA AAATTTGACA ACTTTGGCTT CGGGACTGAC
    ATTGGGATCC CATCTTCTGC TGATGTGGAT TTAGGTTCTG GCCATCATCA AAGCACTGAG
    GAGCAGGAAG TGGCCAGCCT AACTACGCTT CATTTAGATT CAGAAACAAG CAGCCTTAAT
    CAGCAAGCCT TCTCTGCAGA AGTTGCAACC GTTACTGGTT CAGAAAGTGC TTCCCCCGTT
    CATTCAGCTC TGGGATCCAG GTCGCAGACC CCCTCCCCAT CTACACTGAG CAGAGCTCAC
    ATAGAACAGA AGGACCTACA GCTCGATGAG AAGCTCCACC ACTCTGTTCT TCAGACGCCG
    GATGACCTAG AAATCAGTGA ATTTCCATCT GAATGTTGTA GCGTGATGGC AGGAGGTACT
    CTCACTGGGT GGCATGCTGA TGTTGCTACT GTTATGTGGC GCAGAATGCT GGGCATTTTG
    GGAGATGTCA ATGCTATTAT GGATCCTGAA ATACATGCTC AAGTTTTTGA TTACCTCTGT
    GAACTTTGGC AGAATCTAGC CAAGATCAGA GATAACCTTG GCATTTCAGC TGACAACCTG
    ACTTCCCCTT CTCCACCCGT CCTGATTCCT CCACTAAGAA TTCTTACACC TTGGCTTTTT
    AAGGCAACCA TGTTGACTGA TAAGTATAAA CAAGGTAAAT TACATGCATA TAAACTTATT
    TGTAATACAA TGAAGAGAAG ACAAGATGTA TCTCCAAATA GAGATTTTTT AACACATTTC
    TACAATATAA TGCATTGTGG ATTGCTTCAT ATTGACCAGG ACATTGTCAA TACGATCATC
    AAGCACTGCT CACCTCAGTT TTTTTCACTC GGTTTGCCTG GGGCCACGAT GCTTATTATG
    GATTTTATTA TAGCAGCTGG GAGAGTGGCT TCTTCGGCTT TTCTTAATGC ACCAAGAGTG
    GAAGCACAAG TTCTTCTGGG ATCTTTAGTT TGCTTTCCCA ACTTATATTG TGAATTGCCT
    GCTCTCCATC CCAACATTCC TGATATTGCT GTGTCTCAAT TTACAGATGT TAAGGAACTT
    ATAATTAAAA CTGTATTAAG CTCGGCAAGA GATGAGCCCT CTGGTCCTGC ACGATGTGTA
    GCACTTTGCA GTTTAGGTAT TTGGATTTGT GAAGAATTAG TCCACGAATC TCATCATCCT
    CAAATTAAAG AAGCACTGAA TGTAATTTGT GTTTCATTAA AGTTTACTAA TAAAACAGTT
    GCCCACGTCG CCTGTAACAT GCTTCACATG CTGGTTCACT ATGTCCCTAG ACTTCAGATT
    CACCAGCCGC AGTCTCCCCT GAAAATTATT CAGATCCTAA TAGCAACTAT CACCCACCTT
    TTGCCAAGTA CAGAAGCTTC ATCATATGAA ATGGACAAGA GGTTGGTTGT CTCATTACTT
    CTTTGCCTTC TGGACTGGAT CATGGCCTTA CCTTTAAAGA CACTGCTCCA ACCAGTCCAT
    GCTACAGGAG CAGAAAATGA TAAGACCGAA AAGTCTGTTC TCAACTGCAT TTATAAGGTA
    TTACATGGGT GTGTCTATGG AGCGCAAAGT TTTAGCAATC CCAAGTACTT TCCAATAAGC
    CTTTCTGATT TGGCATCTGT AGATTATGAT CCGTTTATGC ATTTGGAAAG TCTGAAAGAA
    CCTGAACCTT TACACTCTCC AGATTCAGAG CGGTCTTCTA AACTGCAGCC TGTGACAGAA
    GTGAAAACTC AGATGCAACA AGGATTAATC TCTATAGCAG CCCGTACTGT TATTACCCAT
    CTGGTGAATC ACTTGGGCCA TTATCCAATG AGTGGTGGTC CTGCTATGCT AACAAGTCAG
    GTGTGTGAAA ATCATGACAA TCATTACAGT GAAAGTACTG AACTTTCTCC TGAGCTCTTT
    GAAAGTCCAA ACATCCAGTT CTTTGTGCTG AATAATACAA CCTTAGTGTC CTGTATCCAG
    ATAAGATCGG AAGAGAGTAT GCCTGGAGGA GGTCTAGCTG CTGGCCTTGT GTCAGCCAAC
    TCAAATGTTA GAATCATAGT ACGTGATCTC TCTGGAAAGT ACTCATGGGA TTCTGCCATA
    CTGTATGGAC CACCCATTGT AAGTGGTTTG CCTGAACCTA CATCTTTCAT ACTTTCAATG
    TCTGACCAAG AGAAACCGGA AGAGCCTCCT ACATCTAACG AATGCTTAGA AGACATCGCA
    GTAAAAGATG GGCTTTCTCT CCAGCTTAGA AGATTTAGAG AAACTGTACC AACTTGGTCT
    ACGATACGAG AGGAAGAAGA TGTTCTTGAC GAGCTCCTGC AGTATTTAGG CACTACAAGT
    CCTGAGTGTT TACAGAGAAC GGGAATCTCC CTCAATGTTC CTGCTCCACA GCCTCTGTGC
    ATTTCTGAAA AACAGGAGAA TGATGTCATT AATGCTATCC TTAAGCAATA CACGGAAGAA
    AAAGAGTTTG TAGAGAAGCA CTTTAATGAC TTAAACATGA AAGCCTCAGA ACAAGATGAG
    CCAACACCTC AGAAACCTCA GTCAGCTTTT TATTACTGCA GATTGCTTCT TAGTATATTG
    GGAATGAATT CCTGGGACAA ACGGAGGAGC TTTCATCTCT TGAAGAAAAA TGAAAAGCTG
    CTCAGAGAAC TTAGGAACTT GGATTCAAGG CAATGCCGAG AGACACACAA GATTGCAGTA
    TTTTACGTTG CTGAAGGACA AGAAGATAAA TACTCCATTC TCACCAATAT AGGAGGAAGC
    CAAGCATATG AAGATTTTGT AGCTGGCCTT GGTTGGGAGG TAAACCTTAC AAACCACTGT
    GGCTTCATGG GAGGCCTTCA GAAGAACAGA AGCACTGGAC TGACCACTCC CTATTTTGCT
    ACCTCGACAG TAGAAGTAAT ATTTCATGTA TCTACACGAA TGCCTTCTGA TTCTGATGAC
    TCTCTGACCA AAAAATTGAG ACATCTAGGA AATGATGAAG TGCACATTGT TTGGTCAGAA
    CATACTAGAG ACTACAGGAG AGGAATTATT CCTACTGAGT TTGGTGATGT CCTCATTGTA
    ATATACCCAA TGAAAAACCA CATGTTCAGT ATCCAGATCA TGAAAAAACC AGAGGTTCCC
    TTCTTTGGTC CACTTTTTGA TGGTGCTATT GTGAATGGAA AAGTTCTGCC CATTATGGTT
    CGATCCACAG CAATAAATGC TAGCCGTGCT CTGAAATCAC TGATTCCATT GTATCAAAAT
    TTCTATGAGG AGAGAGCACG GTACCTGCAG ACAATTGTCC AGCACCATTT AGAGCCAACA
    ACATTTGAAG ACTTTGCAGC ACAGGTTTTC TCTCCAGCTC CCTACCATCA TTTCCCTGCT
    GACGCAGTTG GATCCTACCC AGAGATTCCA CCCAGTGACG CTGCCCCAGC AGCACAGGTA
    GACGGGGCTG ACCTGGCCTC TCCCATGTCT CCTCGAACTA GCAAAAGCCG CATGTCCATG
    AAGCTGCGTC GGTCCTCTGG CTCAGCCAAT AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu84575
    Clone ID Related Accession (Same CDS sequence) NM_001368814.1
    Accession Version NM_001368814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159624.2, AC107633.11 and AC158398.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGGTGCAGTT
    CCTGTCGTTG ACAGTTCATC CCGTCATGCT CCAAGCTTGC AGAGTTCCAC AGAGGCTTCG
    TCAATAACTA GATCCACTGA GAGCCACCTC ACTGATACTC ATAGTAGAGA GTCTTCTCTG
    GAAGTTGGTG ATAGCATATA TGACCATCTT TGCCATTTGA TAGGTCCAGT AGAACTTGCA
    GATGCAGCTT TGGATCAAAT CCAGTACATT GACCTTGAAG GAGACGATGA CCTTCTTTCT
    TCTCTGAAAG AATACTTTAA GGAAAACCAG GAAAGTCGTA GTAAAAGTGA GCTAGGGAAA
    GATACAGCTT CCCAGGAAGT GCTTATTGAC GTAACTAGAG AGGAAATACC ATCTTTCCAT
    AAAGTAGAGT ACACAGGTCA GGAAGTAAAA CCAGAAAATC TCATGTCTTT TGTTGGGACT
    TCTGAAAATA CACCATTTCA AAAAGAACCA AGCTCAGCTG TCTTCACAAG TACTCTTGCA
    CCTAACCATT CAGATAGCTA TCCAAGATCA CAAACTGCTG AAAAGGCTAA GCAACTAAGT
    CTTGATGAAC ACTCATCGGA TCCTAGCTCA GATAGCCTAT GTGATAAAGA AAAAAAGAAA
    CATCTGTGTA GACAAACAGC CACAGAAGTA GATGCCTGTG TAGATATGAC AGTAGCTGAA
    AAGCTTAAGA GTGTACAGGT TAATGAAAAA ATCACGCCAT ACTTAATGCA TGCCAAGAAA
    ACTGCATTAA AAACACCGGT AAGCCGCAGA ATGCCTCATG TTTCAAACAC TAGCAAGCTT
    TCTCCAACTA AGAGGAGCTT GTCTGAAACA GTAACTCATA GGGCCAAAAT CATGAAAATT
    GCCGCTAAGA AACGAAATAG TGTTCATGTT ACTTTTAGAC CATCTACCGA GTCTGTCCAA
    TTTTATAATC CTCTGGAAAA CAAAGAGGCT CCATGGAAGA TGAGACTACG GAAGCTCAGT
    GGCTTTAGCA GTAATAGCAG TAGCACCACC AGCAACACAC AGACCAGCTC AACTAGTGGT
    ACAGACCTTG TAAAGCCTGG TGTGTACAGA CCTCTAGATA CAATCGGTGC AGCATCAGTT
    AGCAGAGCAG TGAGGGAATC CACAGAGATC CCCACAGCCA CATCACAGAA AGAAGGAATG
    GCAAGTAACC AGGCAGGTAG TCGCAGTACT CTCAGGTCAT CGAGTCAGGA GACAGGACCA
    CAGCAGGGCT CCCTGGGTGG CGTTTATAAA ACTGTTGTCC ATGCTCTTTC GAAGCCGAAG
    GCAAATGTTT CCCCACAGAG GCAGAACAGA ATGCCACCCG AGGCTCCACT GAGGGATCTG
    TACAGTCATG TAATGGGCTA TTTTGGAAGG AAAGCTGCAG TAAATAAAGA GGACACGAGC
    CCCAAACTGC CTCCTCTTAA TAGTGAGACT GGTGGCAACA GCGCCAATGT TCCTGACCTG
    ATGGATGAGT TTATCGCAGA GCGACTTCGA AGTGGCAATG CCTCGACTAT GACAAGAAGG
    GGGAGTAGTC CAGGCAGCCT TGAAATCCCG AAAGACCTCC CTGATATTCT AAACAAGCAG
    AACCAGATGC GGCCTGTTGA TGACCCTGGT GTACCCTCCG AATGGACGTC TCCAGCCAGT
    GCAGGGAGCA GTGATCTCAT GAGCTCAGAT AGTCACTCGG ATTCCTTCAG TGCCTTCCAG
    TGTGAGGGAC GAAAATTTGA CAACTTTGGC TTCGGGACTG ACATTGGGAT CCCATCTTCT
    GCTGATGTGG ATTTAGGTTC TGGCCATCAT CAAAGCACTG AGGAGCAGGA AGTGGCCAGC
    CTAACTACGC TTCATTTAGA TTCAGAAACA AGCAGCCTTA ATCAGCAAGC CTTCTCTGCA
    GAAGTTGCAA CCGTTACTGG TTCAGAAAGT GCTTCCCCCG TTCATTCAGC TCTGGGATCC
    AGGTCGCAGA CCCCCTCCCC ATCTACACTG AGCAGAGCTC ACATAGAACA GAAGGACCTA
    CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACGC CGGATGACCT AGAAATCAGT
    GAATTTCCAT CTGAATGTTG TAGCGTGATG GCAGGAGGTA CTCTCACTGG GTGGCATGCT
    GATGTTGCTA CTGTTATGTG GCGCAGAATG CTGGGCATTT TGGGAGATGT CAATGCTATT
    ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA
    GCCAAGATCA GAGATAACCT TGGCATTTCA GCTGACAACC TGACTTCCCC TTCTCCACCC
    GTCCTGATTC CTCCACTAAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTGACT
    GATAAGTATA AACAAGGTAA ATTACATGCA TATAAACTTA TTTGTAATAC AATGAAGAGA
    AGACAAGATG TATCTCCAAA TAGAGATTTT TTAACACATT TCTACAATAT AATGCATTGT
    GGATTGCTTC ATATTGACCA GGACATTGTC AATACGATCA TCAAGCACTG CTCACCTCAG
    TTTTTTTCAC TCGGTTTGCC TGGGGCCACG ATGCTTATTA TGGATTTTAT TATAGCAGCT
    GGGAGAGTGG CTTCTTCGGC TTTTCTTAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG
    GGATCTTTAG TTTGCTTTCC CAACTTATAT TGTGAATTGC CTGCTCTCCA TCCCAACATT
    CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATTAA AACTGTATTA
    AGCTCGGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT
    ATTTGGATTT GTGAAGAATT AGTCCACGAA TCTCATCATC CTCAAATTAA AGAAGCACTG
    AATGTAATTT GTGTTTCATT AAAGTTTACT AATAAAACAG TTGCCCACGT CGCCTGTAAC
    ATGCTTCACA TGCTGGTTCA CTATGTCCCT AGACTTCAGA TTCACCAGCC GCAGTCTCCC
    CTGAAAATTA TTCAGATCCT AATAGCAACT ATCACCCACC TTTTGCCAAG TACAGAAGCT
    TCATCATATG AAATGGACAA GAGGTTGGTT GTCTCATTAC TTCTTTGCCT TCTGGACTGG
    ATCATGGCCT TACCTTTAAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT
    GATAAGACCG AAAAGTCTGT TCTCAACTGC ATTTATAAGG TATTACATGG GTGTGTCTAT
    GGAGCGCAAA GTTTTAGCAA TCCCAAGTAC TTTCCAATAA GCCTTTCTGA TTTGGCATCT
    GTAGATTATG ATCCGTTTAT GCATTTGGAA AGTCTGAAAG AACCTGAACC TTTACACTCT
    CCAGATTCAG AGCGGTCTTC TAAACTGCAG CCTGTGACAG AAGTGAAAAC TCAGATGCAA
    CAAGGATTAA TCTCTATAGC AGCCCGTACT GTTATTACCC ATCTGGTGAA TCACTTGGGC
    CATTATCCAA TGAGTGGTGG TCCTGCTATG CTAACAAGTC AGGTGTGTGA AAATCATGAC
    AATCATTACA GTGAAAGTAC TGAACTTTCT CCTGAGCTCT TTGAAAGTCC AAACATCCAG
    TTCTTTGTGC TGAATAATAC AACCTTAGTG TCCTGTATCC AGATAAGATC GGAAGAGAGT
    ATGCCTGGAG GAGGTCTAGC TGCTGGCCTT GTGTCAGCCA ACTCAAATGT TAGAATCATA
    GTACGTGATC TCTCTGGAAA GTACTCATGG GATTCTGCCA TACTGTATGG ACCACCCATT
    GTAAGTGGTT TGCCTGAACC TACATCTTTC ATACTTTCAA TGTCTGACCA AGAGAAACCG
    GAAGAGCCTC CTACATCTAA CGAATGCTTA GAAGACATCG CAGTAAAAGA TGGGCTTTCT
    CTCCAGCTTA GAAGATTTAG AGAAACTGTA CCAACTTGGT CTACGATACG AGAGGAAGAA
    GATGTTCTTG ACGAGCTCCT GCAGTATTTA GGCACTACAA GTCCTGAGTG TTTACAGAGA
    ACGGGAATCT CCCTCAATGT TCCTGCTCCA CAGCCTCTGT GCATTTCTGA AAAACAGGAG
    AATGATGTCA TTAATGCTAT CCTTAAGCAA TACACGGAAG AAAAAGAGTT TGTAGAGAAG
    CACTTTAATG ACTTAAACAT GAAAGCCTCA GAACAAGATG AGCCAACACC TCAGAAACCT
    CAGTCAGCTT TTTATTACTG CAGATTGCTT CTTAGTATAT TGGGAATGAA TTCCTGGGAC
    AAACGGAGGA GCTTTCATCT CTTGAAGAAA AATGAAAAGC TGCTCAGAGA ACTTAGGAAC
    TTGGATTCAA GGCAATGCCG AGAGACACAC AAGATTGCAG TATTTTACGT TGCTGAAGGA
    CAAGAAGATA AATACTCCAT TCTCACCAAT ATAGGAGGAA GCCAAGCATA TGAAGATTTT
    GTAGCTGGCC TTGGTTGGGA GGTAAACCTT ACAAACCACT GTGGCTTCAT GGGAGGCCTT
    CAGAAGAACA GAAGCACTGG ACTGACCACT CCCTATTTTG CTACCTCGAC AGTAGAAGTA
    ATATTTCATG TATCTACACG AATGCCTTCT GATTCTGATG ACTCTCTGAC CAAAAAATTG
    AGACATCTAG GAAATGATGA AGTGCACATT GTTTGGTCAG AACATACTAG AGACTACAGG
    AGAGGAATTA TTCCTACTGA GTTTGGTGAT GTCCTCATTG TAATATACCC AATGAAAAAC
    CACATGTTCA GTATCCAGAT CATGAAAAAA CCAGAGGTTC CCTTCTTTGG TCCACTTTTT
    GATGGTGCTA TTGTGAATGG AAAAGTTCTG CCCATTATGG TTCGATCCAC AGCAATAAAT
    GCTAGCCGTG CTCTGAAATC ACTGATTCCA TTGTATCAAA ATTTCTATGA GGAGAGAGCA
    CGGTACCTGC AGACAATTGT CCAGCACCAT TTAGAGCCAA CAACATTTGA AGACTTTGCA
    GCACAGGTTT TCTCTCCAGC TCCCTACCAT CATTTCCCTG CTGACGCAGA TCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001355743.1
    CDS401..8017
    Translation

    Target ORF information:

    RefSeq Version NM_001368814.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001368814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGGTGCAGTT
    CCTGTCGTTG ACAGTTCATC CCGTCATGCT CCAAGCTTGC AGAGTTCCAC AGAGGCTTCG
    TCAATAACTA GATCCACTGA GAGCCACCTC ACTGATACTC ATAGTAGAGA GTCTTCTCTG
    GAAGTTGGTG ATAGCATATA TGACCATCTT TGCCATTTGA TAGGTCCAGT AGAACTTGCA
    GATGCAGCTT TGGATCAAAT CCAGTACATT GACCTTGAAG GAGACGATGA CCTTCTTTCT
    TCTCTGAAAG AATACTTTAA GGAAAACCAG GAAAGTCGTA GTAAAAGTGA GCTAGGGAAA
    GATACAGCTT CCCAGGAAGT GCTTATTGAC GTAACTAGAG AGGAAATACC ATCTTTCCAT
    AAAGTAGAGT ACACAGGTCA GGAAGTAAAA CCAGAAAATC TCATGTCTTT TGTTGGGACT
    TCTGAAAATA CACCATTTCA AAAAGAACCA AGCTCAGCTG TCTTCACAAG TACTCTTGCA
    CCTAACCATT CAGATAGCTA TCCAAGATCA CAAACTGCTG AAAAGGCTAA GCAACTAAGT
    CTTGATGAAC ACTCATCGGA TCCTAGCTCA GATAGCCTAT GTGATAAAGA AAAAAAGAAA
    CATCTGTGTA GACAAACAGC CACAGAAGTA GATGCCTGTG TAGATATGAC AGTAGCTGAA
    AAGCTTAAGA GTGTACAGGT TAATGAAAAA ATCACGCCAT ACTTAATGCA TGCCAAGAAA
    ACTGCATTAA AAACACCGGT AAGCCGCAGA ATGCCTCATG TTTCAAACAC TAGCAAGCTT
    TCTCCAACTA AGAGGAGCTT GTCTGAAACA GTAACTCATA GGGCCAAAAT CATGAAAATT
    GCCGCTAAGA AACGAAATAG TGTTCATGTT ACTTTTAGAC CATCTACCGA GTCTGTCCAA
    TTTTATAATC CTCTGGAAAA CAAAGAGGCT CCATGGAAGA TGAGACTACG GAAGCTCAGT
    GGCTTTAGCA GTAATAGCAG TAGCACCACC AGCAACACAC AGACCAGCTC AACTAGTGGT
    ACAGACCTTG TAAAGCCTGG TGTGTACAGA CCTCTAGATA CAATCGGTGC AGCATCAGTT
    AGCAGAGCAG TGAGGGAATC CACAGAGATC CCCACAGCCA CATCACAGAA AGAAGGAATG
    GCAAGTAACC AGGCAGGTAG TCGCAGTACT CTCAGGTCAT CGAGTCAGGA GACAGGACCA
    CAGCAGGGCT CCCTGGGTGG CGTTTATAAA ACTGTTGTCC ATGCTCTTTC GAAGCCGAAG
    GCAAATGTTT CCCCACAGAG GCAGAACAGA ATGCCACCCG AGGCTCCACT GAGGGATCTG
    TACAGTCATG TAATGGGCTA TTTTGGAAGG AAAGCTGCAG TAAATAAAGA GGACACGAGC
    CCCAAACTGC CTCCTCTTAA TAGTGAGACT GGTGGCAACA GCGCCAATGT TCCTGACCTG
    ATGGATGAGT TTATCGCAGA GCGACTTCGA AGTGGCAATG CCTCGACTAT GACAAGAAGG
    GGGAGTAGTC CAGGCAGCCT TGAAATCCCG AAAGACCTCC CTGATATTCT AAACAAGCAG
    AACCAGATGC GGCCTGTTGA TGACCCTGGT GTACCCTCCG AATGGACGTC TCCAGCCAGT
    GCAGGGAGCA GTGATCTCAT GAGCTCAGAT AGTCACTCGG ATTCCTTCAG TGCCTTCCAG
    TGTGAGGGAC GAAAATTTGA CAACTTTGGC TTCGGGACTG ACATTGGGAT CCCATCTTCT
    GCTGATGTGG ATTTAGGTTC TGGCCATCAT CAAAGCACTG AGGAGCAGGA AGTGGCCAGC
    CTAACTACGC TTCATTTAGA TTCAGAAACA AGCAGCCTTA ATCAGCAAGC CTTCTCTGCA
    GAAGTTGCAA CCGTTACTGG TTCAGAAAGT GCTTCCCCCG TTCATTCAGC TCTGGGATCC
    AGGTCGCAGA CCCCCTCCCC ATCTACACTG AGCAGAGCTC ACATAGAACA GAAGGACCTA
    CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACGC CGGATGACCT AGAAATCAGT
    GAATTTCCAT CTGAATGTTG TAGCGTGATG GCAGGAGGTA CTCTCACTGG GTGGCATGCT
    GATGTTGCTA CTGTTATGTG GCGCAGAATG CTGGGCATTT TGGGAGATGT CAATGCTATT
    ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA
    GCCAAGATCA GAGATAACCT TGGCATTTCA GCTGACAACC TGACTTCCCC TTCTCCACCC
    GTCCTGATTC CTCCACTAAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTGACT
    GATAAGTATA AACAAGGTAA ATTACATGCA TATAAACTTA TTTGTAATAC AATGAAGAGA
    AGACAAGATG TATCTCCAAA TAGAGATTTT TTAACACATT TCTACAATAT AATGCATTGT
    GGATTGCTTC ATATTGACCA GGACATTGTC AATACGATCA TCAAGCACTG CTCACCTCAG
    TTTTTTTCAC TCGGTTTGCC TGGGGCCACG ATGCTTATTA TGGATTTTAT TATAGCAGCT
    GGGAGAGTGG CTTCTTCGGC TTTTCTTAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG
    GGATCTTTAG TTTGCTTTCC CAACTTATAT TGTGAATTGC CTGCTCTCCA TCCCAACATT
    CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATTAA AACTGTATTA
    AGCTCGGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT
    ATTTGGATTT GTGAAGAATT AGTCCACGAA TCTCATCATC CTCAAATTAA AGAAGCACTG
    AATGTAATTT GTGTTTCATT AAAGTTTACT AATAAAACAG TTGCCCACGT CGCCTGTAAC
    ATGCTTCACA TGCTGGTTCA CTATGTCCCT AGACTTCAGA TTCACCAGCC GCAGTCTCCC
    CTGAAAATTA TTCAGATCCT AATAGCAACT ATCACCCACC TTTTGCCAAG TACAGAAGCT
    TCATCATATG AAATGGACAA GAGGTTGGTT GTCTCATTAC TTCTTTGCCT TCTGGACTGG
    ATCATGGCCT TACCTTTAAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT
    GATAAGACCG AAAAGTCTGT TCTCAACTGC ATTTATAAGG TATTACATGG GTGTGTCTAT
    GGAGCGCAAA GTTTTAGCAA TCCCAAGTAC TTTCCAATAA GCCTTTCTGA TTTGGCATCT
    GTAGATTATG ATCCGTTTAT GCATTTGGAA AGTCTGAAAG AACCTGAACC TTTACACTCT
    CCAGATTCAG AGCGGTCTTC TAAACTGCAG CCTGTGACAG AAGTGAAAAC TCAGATGCAA
    CAAGGATTAA TCTCTATAGC AGCCCGTACT GTTATTACCC ATCTGGTGAA TCACTTGGGC
    CATTATCCAA TGAGTGGTGG TCCTGCTATG CTAACAAGTC AGGTGTGTGA AAATCATGAC
    AATCATTACA GTGAAAGTAC TGAACTTTCT CCTGAGCTCT TTGAAAGTCC AAACATCCAG
    TTCTTTGTGC TGAATAATAC AACCTTAGTG TCCTGTATCC AGATAAGATC GGAAGAGAGT
    ATGCCTGGAG GAGGTCTAGC TGCTGGCCTT GTGTCAGCCA ACTCAAATGT TAGAATCATA
    GTACGTGATC TCTCTGGAAA GTACTCATGG GATTCTGCCA TACTGTATGG ACCACCCATT
    GTAAGTGGTT TGCCTGAACC TACATCTTTC ATACTTTCAA TGTCTGACCA AGAGAAACCG
    GAAGAGCCTC CTACATCTAA CGAATGCTTA GAAGACATCG CAGTAAAAGA TGGGCTTTCT
    CTCCAGCTTA GAAGATTTAG AGAAACTGTA CCAACTTGGT CTACGATACG AGAGGAAGAA
    GATGTTCTTG ACGAGCTCCT GCAGTATTTA GGCACTACAA GTCCTGAGTG TTTACAGAGA
    ACGGGAATCT CCCTCAATGT TCCTGCTCCA CAGCCTCTGT GCATTTCTGA AAAACAGGAG
    AATGATGTCA TTAATGCTAT CCTTAAGCAA TACACGGAAG AAAAAGAGTT TGTAGAGAAG
    CACTTTAATG ACTTAAACAT GAAAGCCTCA GAACAAGATG AGCCAACACC TCAGAAACCT
    CAGTCAGCTT TTTATTACTG CAGATTGCTT CTTAGTATAT TGGGAATGAA TTCCTGGGAC
    AAACGGAGGA GCTTTCATCT CTTGAAGAAA AATGAAAAGC TGCTCAGAGA ACTTAGGAAC
    TTGGATTCAA GGCAATGCCG AGAGACACAC AAGATTGCAG TATTTTACGT TGCTGAAGGA
    CAAGAAGATA AATACTCCAT TCTCACCAAT ATAGGAGGAA GCCAAGCATA TGAAGATTTT
    GTAGCTGGCC TTGGTTGGGA GGTAAACCTT ACAAACCACT GTGGCTTCAT GGGAGGCCTT
    CAGAAGAACA GAAGCACTGG ACTGACCACT CCCTATTTTG CTACCTCGAC AGTAGAAGTA
    ATATTTCATG TATCTACACG AATGCCTTCT GATTCTGATG ACTCTCTGAC CAAAAAATTG
    AGACATCTAG GAAATGATGA AGTGCACATT GTTTGGTCAG AACATACTAG AGACTACAGG
    AGAGGAATTA TTCCTACTGA GTTTGGTGAT GTCCTCATTG TAATATACCC AATGAAAAAC
    CACATGTTCA GTATCCAGAT CATGAAAAAA CCAGAGGTTC CCTTCTTTGG TCCACTTTTT
    GATGGTGCTA TTGTGAATGG AAAAGTTCTG CCCATTATGG TTCGATCCAC AGCAATAAAT
    GCTAGCCGTG CTCTGAAATC ACTGATTCCA TTGTATCAAA ATTTCTATGA GGAGAGAGCA
    CGGTACCTGC AGACAATTGT CCAGCACCAT TTAGAGCCAA CAACATTTGA AGACTTTGCA
    GCACAGGTTT TCTCTCCAGC TCCCTACCAT CATTTCCCTG CTGACGCAGA TCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48011
    Clone ID Related Accession (Same CDS sequence) XM_006516104.2 , XM_006516104.3
    Accession Version XM_006516104.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516104.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GTTGGATCCT ACCCAGAGAT TCCACCCAGT GACGCTGCCC CAGCAGCACA GGTAGACGGG
    GCTGACCTGG CCTCTCCCAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGGTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516167.1
    CDS808..7200
    Translation

    Target ORF information:

    RefSeq Version XM_006516104.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516104.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GTTGGATCCT ACCCAGAGAT TCCACCCAGT GACGCTGCCC CAGCAGCACA GGTAGACGGG
    GCTGACCTGG CCTCTCCCAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGGTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48013
    Clone ID Related Accession (Same CDS sequence) XM_006516107.2 , XM_006516107.3
    Accession Version XM_006516107.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516107.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG GATCCTACCC AGAGATTCCA
    CCCAGTGACG CTGCCCCAGC AGCACAGGTA GACGGGGCTG ACCTGGCCTC TCCCATGTCT
    CCTCGAACTA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GGTCCTCTGG CTCAGCCAAT
    AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516170.1
    CDS808..7056
    Translation

    Target ORF information:

    RefSeq Version XM_006516107.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516107.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG GATCCTACCC AGAGATTCCA
    CCCAGTGACG CTGCCCCAGC AGCACAGGTA GACGGGGCTG ACCTGGCCTC TCCCATGTCT
    CCTCGAACTA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GGTCCTCTGG CTCAGCCAAT
    AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86114
    Clone ID Related Accession (Same CDS sequence) XM_030246825.1
    Accession Version XM_030246825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGGTGCAGTT
    CCTGTCGTTG ACAGTTCATC CCGTCATGCT CCAAGCTTGC AGAGTTCCAC AGAGGCTTCG
    TCAATAACTA GATCCACTGA GAGCCACCTC ACTGATACTC ATAGTAGAGA GTCTTCTCTG
    GAAGTTGGTG ATAGCATATA TGACCATCTT TGCCATTTGA TAGGTCCAGT AGAACTTGCA
    GATGCAGCTT TGGATCAAAT CCAGTACATT GACCTTGAAG GAGACGATGA CCTTCTTTCT
    TCTCTGAAAG AATACTTTAA GGAAAACCAG GAAAGTCGTA GTAAAAGTGA GCTAGGGAAA
    GATACAGCTT CCCAGGAAGT GCTTATTGAC GTAACTAGAG AGGAAATACC ATCTTTCCAT
    AAAGTAGAGT ACACAGGTCA GGAAGTAAAA CCAGAAAATC TCATGTCTTT TGTTGGGACT
    TCTGAAAATA CACCATTTCA AAAAGAACCA AGCTCAGCTG TCTTCACAAG TACTCTTGCA
    CCTAACCATT CAGATAGCTA TCCAAGATCA CAAACTGCTG AAAAGGCTAA GCAACTAAGT
    CTTGATGAAC ACTCATCGGA TCCTAGCTCA GATAGCCTAT GTGATAAAGA AAAAAAGAAA
    CATCTGTGTA GACAAACAGC CACAGAAGTA GATGCCTGTG TAGATATGAC AGTAGCTGAA
    AAGCTTAAGA GTGTACAGGT TAATGAAAAA ATCACGCCAT ACTTAATGCA TGCCAAGAAA
    ACTGCATTAA AAACACCGGT AAGCCGCAGA ATGCCTCATG TTTCAAACAC TAGCAAGCTT
    TCTCCAACTA AGAGGAGCTT GTCTGAAACA GTAACTCATA GGGCCAAAAT CATGAAAATT
    GCCGCTAAGA AACGAAATAG TGTTCATGTT ACTTTTAGAC CATCTACCGA GTCTGTCCAA
    TTTTATAATC CTCTGGAAAA CAAAGAGGCT CCATGGAAGA TGAGACTACG GAAGCTCAGT
    GGCTTTAGCA GTAATAGCAG TAGCACCACC AGCAACACAC AGACCAGCTC AACTAGTGGT
    ACAGACCTTG TAAAGCCTGG TGTGTACAGA CCTCTAGATA CAATCGGTGC AGCATCAGTT
    AGCAGAGCAG TGAGGGAATC CACAGAGATC CCCACAGCCA CATCACAGAA AGAAGGAATG
    GCAAGTAACC AGGCAGGTAG TCGCAGTACT CTCAGGTCAT CGAGTCAGGA GACAGGACCA
    CAGCAGGGCT CCCTGGGTGG CGTTTATAAA ACTGTTGTCC ATGCTCTTTC GAAGCCGAAG
    GCAAATGTTT CCCCACAGAG GCAGAACAGA ATGCCACCCG AGGCTCCACT GAGGGATCTG
    TACAGTCATG TAATGGGCTA TTTTGGAAGG AAAGCTGCAG TAAATAAAGA GGACACGAGC
    CCCAAACTGC CTCCTCTTAA TAGTGAGACT GGTGGCAACA GCGCCAATGT TCCTGACCTG
    ATGGATGAGT TTATCGCAGA GCGACTTCGA AGTGGCAATG CCTCGACTAT GACAAGAAGG
    GGGAGTAGTC CAGGCAGCCT TGAAATCCCG AAAGACCTCC CTGATATTCT AAACAAGCAG
    AACCAGATGC GGCCTGTTGA TGACCCTGGT GTACCCTCCG AATGGACGTC TCCAGCCAGT
    GCAGGGAGCA GTGATCTCAT GAGCTCAGAT AGTCACTCGG ATTCCTTCAG TGCCTTCCAG
    TGTGAGGGAC GAAAATTTGA CAACTTTGGC TTCGGGACTG ACATTGGGAT CCCATCTTCT
    GCTGATGTGG ATTTAGGTTC TGGCCATCAT CAAAGCACTG AGGAGCAGGA AGTGGCCAGC
    CTAACTACGC TTCATTTAGA TTCAGAAACA AGCAGCCTTA ATCAGCAAGC CTTCTCTGCA
    GAAGTTGCAA CCGTTACTGG TTCAGAAAGT GCTTCCCCCG TTCATTCAGC TCTGGGATCC
    AGGTCGCAGA CCCCCTCCCC ATCTACACTG AGCAGAGCTC ACATAGAACA GAAGGACCTA
    CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACGC CGGATGACCT AGAAATCAGT
    GAATTTCCAT CTGAATGTTG TAGCGTGATG GCAGGAGGTA CTCTCACTGG GTGGCATGCT
    GATGTTGCTA CTGTTATGTG GCGCAGAATG CTGGGCATTT TGGGAGATGT CAATGCTATT
    ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA
    GCCAAGATCA GAGATAACCT TGGCATTTCA GCTGACAACC TGACTTCCCC TTCTCCACCC
    GTCCTGATTC CTCCACTAAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTGACT
    GATAAGTATA AACAAGGTAA ATTACATGCA TATAAACTTA TTTGTAATAC AATGAAGAGA
    AGACAAGATG TATCTCCAAA TAGAGATTTT TTAACACATT TCTACAATAT AATGCATTGT
    GGATTGCTTC ATATTGACCA GGACATTGTC AATACGATCA TCAAGCACTG CTCACCTCAG
    TTTTTTTCAC TCGGTTTGCC TGGGGCCACG ATGCTTATTA TGGATTTTAT TATAGCAGCT
    GGGAGAGTGG CTTCTTCGGC TTTTCTTAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG
    GGATCTTTAG TTTGCTTTCC CAACTTATAT TGTGAATTGC CTGCTCTCCA TCCCAACATT
    CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATTAA AACTGTATTA
    AGCTCGGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT
    ATTTGGATTT GTGAAGAATT AGTCCACGAA TCTCATCATC CTCAAATTAA AGAAGCACTG
    AATGTAATTT GTGTTTCATT AAAGTTTACT AATAAAACAG TTGCCCACGT CGCCTGTAAC
    ATGCTTCACA TGCTGGTTCA CTATGTCCCT AGACTTCAGA TTCACCAGCC GCAGTCTCCC
    CTGAAAATTA TTCAGATCCT AATAGCAACT ATCACCCACC TTTTGCCAAG TACAGAAGCT
    TCATCATATG AAATGGACAA GAGGTTGGTT GTCTCATTAC TTCTTTGCCT TCTGGACTGG
    ATCATGGCCT TACCTTTAAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT
    GATAAGACCG AAAAGTCTGT TCTCAACTGC ATTTATAAGG TATTACATGG GTGTGTCTAT
    GGAGCGCAAA GTTTTAGCAA TCCCAAGTAC TTTCCAATAA GCCTTTCTGA TTTGGCATCT
    GTAGATTATG ATCCGTTTAT GCATTTGGAA AGTCTGAAAG AACCTGAACC TTTACACTCT
    CCAGATTCAG AGCGGTCTTC TAAACTGCAG CCTGTGACAG AAGTGAAAAC TCAGATGCAA
    CAAGGATTAA TCTCTATAGC AGCCCGTACT GTTATTACCC ATCTGGTGAA TCACTTGGGC
    CATTATCCAA TGAGTGGTGG TCCTGCTATG CTAACAAGTC AGGTGTGTGA AAATCATGAC
    AATCATTACA GTGAAAGTAC TGAACTTTCT CCTGAGCTCT TTGAAAGTCC AAACATCCAG
    TTCTTTGTGC TGAATAATAC AACCTTAGTG TCCTGTATCC AGATAAGATC GGAAGAGAGT
    ATGCCTGGAG GAGGTCTAGC TGCTGGCCTT GTGTCAGCCA ACTCAAATGT TAGAATCATA
    GTACGTGATC TCTCTGGAAA GTACTCATGG GATTCTGCCA TACTGTATGG ACCACCCATT
    GTAAGTGGTT TGCCTGAACC TACATCTTTC ATACTTTCAA TGTCTGACCA AGAGAAACCG
    GAAGAGCCTC CTACATCTAA CGAATGCTTA GAAGACATCG CAGTAAAAGA TGGGCTTTCT
    CTCCAGCTTA GAAGATTTAG AGAAACTGTA CCAACTTGGT CTACGATACG AGAGGAAGAA
    GATGTTCTTG ACGAGCTCCT GCAGTATTTA GGCACTACAA GTCCTGAGTG TTTACAGAGA
    ACGGGAATCT CCCTCAATGT TCCTGCTCCA CAGCCTCTGT GCATTTCTGA AAAACAGGAG
    AATGATGTCA TTAATGCTAT CCTTAAGCAA TACACGGAAG AAAAAGAGTT TGTAGAGAAG
    CACTTTAATG ACTTAAACAT GAAAGCCTCA GAACAAGATG AGCCAACACC TCAGAAACCT
    CAGTCAGCTT TTTATTACTG CAGATTGCTT CTTAGTATAT TGGGAATGAA TTCCTGGGAC
    AAACGGAGGA GCTTTCATCT CTTGAAGAAA AATGAAAAGC TGCTCAGAGA ACTTAGGAAC
    TTGGATTCAA GGCAATGCCG AGAGACACAC AAGATTGCAG TATTTTACGT TGCTGAAGGA
    CAAGAAGATA AATACTCCAT TCTCACCAAT ATAGGAGGAA GCCAAGCATA TGAAGATTTT
    GTAGCTGGCC TTGGTTGGGA GGTAAACCTT ACAAACCACT GTGGCTTCAT GGGAGGCCTT
    CAGAAGAACA GAAGCACTGG ACTGACCACT CCCTATTTTG CTACCTCGAC AGTAGAAGTA
    ATATTTCATG TATCTACACG AATGCCTTCT GATTCTGATG ACTCTCTGAC CAAAAAATTG
    AGACATCTAG GAAATGATGA AGTGCACATT GTTTGGTCAG AACATACTAG AGACTACAGG
    AGAGGAATTA TTCCTACTGA GTTTGGTGAT GTCCTCATTG TAATATACCC AATGAAAAAC
    CACATGTTCA GTATCCAGAT CATGAAAAAA CCAGAGGTTC CCTTCTTTGG TCCACTTTTT
    GATGGTGCTA TTGTGAATGG AAAAGTTCTG CCCATTATGG TTCGATCCAC AGCAATAAAT
    GCTAGCCGTG CTCTGAAATC ACTGATTCCA TTGTATCAAA ATTTCTATGA GGAGAGAGCA
    CGGTACCTGC AGACAATTGT CCAGCACCAT TTAGAGCCAA CAACATTTGA AGACTTTGCA
    GCACAGGTTT TCTCTCCAGC TCCCTACCAT CATTTCCCTG CTGACGCAGT TGGATCCTAC
    CCAGAGATTC CACCCAGTGA CGCTGCCCCA GCAGCACAGG TAGACGGGGC TGACCTGGCC
    TCTCCCATGT CTCCTCGAAC TAGCAAAAGC CGCATGTCCA TGAAGCTGCG TCGGTCCTCT
    GGCTCAGCCA ATAAATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102685.1
    CDS808..8568
    Translation

    Target ORF information:

    RefSeq Version XM_030246825.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGGTGCAGTT
    CCTGTCGTTG ACAGTTCATC CCGTCATGCT CCAAGCTTGC AGAGTTCCAC AGAGGCTTCG
    TCAATAACTA GATCCACTGA GAGCCACCTC ACTGATACTC ATAGTAGAGA GTCTTCTCTG
    GAAGTTGGTG ATAGCATATA TGACCATCTT TGCCATTTGA TAGGTCCAGT AGAACTTGCA
    GATGCAGCTT TGGATCAAAT CCAGTACATT GACCTTGAAG GAGACGATGA CCTTCTTTCT
    TCTCTGAAAG AATACTTTAA GGAAAACCAG GAAAGTCGTA GTAAAAGTGA GCTAGGGAAA
    GATACAGCTT CCCAGGAAGT GCTTATTGAC GTAACTAGAG AGGAAATACC ATCTTTCCAT
    AAAGTAGAGT ACACAGGTCA GGAAGTAAAA CCAGAAAATC TCATGTCTTT TGTTGGGACT
    TCTGAAAATA CACCATTTCA AAAAGAACCA AGCTCAGCTG TCTTCACAAG TACTCTTGCA
    CCTAACCATT CAGATAGCTA TCCAAGATCA CAAACTGCTG AAAAGGCTAA GCAACTAAGT
    CTTGATGAAC ACTCATCGGA TCCTAGCTCA GATAGCCTAT GTGATAAAGA AAAAAAGAAA
    CATCTGTGTA GACAAACAGC CACAGAAGTA GATGCCTGTG TAGATATGAC AGTAGCTGAA
    AAGCTTAAGA GTGTACAGGT TAATGAAAAA ATCACGCCAT ACTTAATGCA TGCCAAGAAA
    ACTGCATTAA AAACACCGGT AAGCCGCAGA ATGCCTCATG TTTCAAACAC TAGCAAGCTT
    TCTCCAACTA AGAGGAGCTT GTCTGAAACA GTAACTCATA GGGCCAAAAT CATGAAAATT
    GCCGCTAAGA AACGAAATAG TGTTCATGTT ACTTTTAGAC CATCTACCGA GTCTGTCCAA
    TTTTATAATC CTCTGGAAAA CAAAGAGGCT CCATGGAAGA TGAGACTACG GAAGCTCAGT
    GGCTTTAGCA GTAATAGCAG TAGCACCACC AGCAACACAC AGACCAGCTC AACTAGTGGT
    ACAGACCTTG TAAAGCCTGG TGTGTACAGA CCTCTAGATA CAATCGGTGC AGCATCAGTT
    AGCAGAGCAG TGAGGGAATC CACAGAGATC CCCACAGCCA CATCACAGAA AGAAGGAATG
    GCAAGTAACC AGGCAGGTAG TCGCAGTACT CTCAGGTCAT CGAGTCAGGA GACAGGACCA
    CAGCAGGGCT CCCTGGGTGG CGTTTATAAA ACTGTTGTCC ATGCTCTTTC GAAGCCGAAG
    GCAAATGTTT CCCCACAGAG GCAGAACAGA ATGCCACCCG AGGCTCCACT GAGGGATCTG
    TACAGTCATG TAATGGGCTA TTTTGGAAGG AAAGCTGCAG TAAATAAAGA GGACACGAGC
    CCCAAACTGC CTCCTCTTAA TAGTGAGACT GGTGGCAACA GCGCCAATGT TCCTGACCTG
    ATGGATGAGT TTATCGCAGA GCGACTTCGA AGTGGCAATG CCTCGACTAT GACAAGAAGG
    GGGAGTAGTC CAGGCAGCCT TGAAATCCCG AAAGACCTCC CTGATATTCT AAACAAGCAG
    AACCAGATGC GGCCTGTTGA TGACCCTGGT GTACCCTCCG AATGGACGTC TCCAGCCAGT
    GCAGGGAGCA GTGATCTCAT GAGCTCAGAT AGTCACTCGG ATTCCTTCAG TGCCTTCCAG
    TGTGAGGGAC GAAAATTTGA CAACTTTGGC TTCGGGACTG ACATTGGGAT CCCATCTTCT
    GCTGATGTGG ATTTAGGTTC TGGCCATCAT CAAAGCACTG AGGAGCAGGA AGTGGCCAGC
    CTAACTACGC TTCATTTAGA TTCAGAAACA AGCAGCCTTA ATCAGCAAGC CTTCTCTGCA
    GAAGTTGCAA CCGTTACTGG TTCAGAAAGT GCTTCCCCCG TTCATTCAGC TCTGGGATCC
    AGGTCGCAGA CCCCCTCCCC ATCTACACTG AGCAGAGCTC ACATAGAACA GAAGGACCTA
    CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACGC CGGATGACCT AGAAATCAGT
    GAATTTCCAT CTGAATGTTG TAGCGTGATG GCAGGAGGTA CTCTCACTGG GTGGCATGCT
    GATGTTGCTA CTGTTATGTG GCGCAGAATG CTGGGCATTT TGGGAGATGT CAATGCTATT
    ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA
    GCCAAGATCA GAGATAACCT TGGCATTTCA GCTGACAACC TGACTTCCCC TTCTCCACCC
    GTCCTGATTC CTCCACTAAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTGACT
    GATAAGTATA AACAAGGTAA ATTACATGCA TATAAACTTA TTTGTAATAC AATGAAGAGA
    AGACAAGATG TATCTCCAAA TAGAGATTTT TTAACACATT TCTACAATAT AATGCATTGT
    GGATTGCTTC ATATTGACCA GGACATTGTC AATACGATCA TCAAGCACTG CTCACCTCAG
    TTTTTTTCAC TCGGTTTGCC TGGGGCCACG ATGCTTATTA TGGATTTTAT TATAGCAGCT
    GGGAGAGTGG CTTCTTCGGC TTTTCTTAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG
    GGATCTTTAG TTTGCTTTCC CAACTTATAT TGTGAATTGC CTGCTCTCCA TCCCAACATT
    CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATTAA AACTGTATTA
    AGCTCGGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT
    ATTTGGATTT GTGAAGAATT AGTCCACGAA TCTCATCATC CTCAAATTAA AGAAGCACTG
    AATGTAATTT GTGTTTCATT AAAGTTTACT AATAAAACAG TTGCCCACGT CGCCTGTAAC
    ATGCTTCACA TGCTGGTTCA CTATGTCCCT AGACTTCAGA TTCACCAGCC GCAGTCTCCC
    CTGAAAATTA TTCAGATCCT AATAGCAACT ATCACCCACC TTTTGCCAAG TACAGAAGCT
    TCATCATATG AAATGGACAA GAGGTTGGTT GTCTCATTAC TTCTTTGCCT TCTGGACTGG
    ATCATGGCCT TACCTTTAAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT
    GATAAGACCG AAAAGTCTGT TCTCAACTGC ATTTATAAGG TATTACATGG GTGTGTCTAT
    GGAGCGCAAA GTTTTAGCAA TCCCAAGTAC TTTCCAATAA GCCTTTCTGA TTTGGCATCT
    GTAGATTATG ATCCGTTTAT GCATTTGGAA AGTCTGAAAG AACCTGAACC TTTACACTCT
    CCAGATTCAG AGCGGTCTTC TAAACTGCAG CCTGTGACAG AAGTGAAAAC TCAGATGCAA
    CAAGGATTAA TCTCTATAGC AGCCCGTACT GTTATTACCC ATCTGGTGAA TCACTTGGGC
    CATTATCCAA TGAGTGGTGG TCCTGCTATG CTAACAAGTC AGGTGTGTGA AAATCATGAC
    AATCATTACA GTGAAAGTAC TGAACTTTCT CCTGAGCTCT TTGAAAGTCC AAACATCCAG
    TTCTTTGTGC TGAATAATAC AACCTTAGTG TCCTGTATCC AGATAAGATC GGAAGAGAGT
    ATGCCTGGAG GAGGTCTAGC TGCTGGCCTT GTGTCAGCCA ACTCAAATGT TAGAATCATA
    GTACGTGATC TCTCTGGAAA GTACTCATGG GATTCTGCCA TACTGTATGG ACCACCCATT
    GTAAGTGGTT TGCCTGAACC TACATCTTTC ATACTTTCAA TGTCTGACCA AGAGAAACCG
    GAAGAGCCTC CTACATCTAA CGAATGCTTA GAAGACATCG CAGTAAAAGA TGGGCTTTCT
    CTCCAGCTTA GAAGATTTAG AGAAACTGTA CCAACTTGGT CTACGATACG AGAGGAAGAA
    GATGTTCTTG ACGAGCTCCT GCAGTATTTA GGCACTACAA GTCCTGAGTG TTTACAGAGA
    ACGGGAATCT CCCTCAATGT TCCTGCTCCA CAGCCTCTGT GCATTTCTGA AAAACAGGAG
    AATGATGTCA TTAATGCTAT CCTTAAGCAA TACACGGAAG AAAAAGAGTT TGTAGAGAAG
    CACTTTAATG ACTTAAACAT GAAAGCCTCA GAACAAGATG AGCCAACACC TCAGAAACCT
    CAGTCAGCTT TTTATTACTG CAGATTGCTT CTTAGTATAT TGGGAATGAA TTCCTGGGAC
    AAACGGAGGA GCTTTCATCT CTTGAAGAAA AATGAAAAGC TGCTCAGAGA ACTTAGGAAC
    TTGGATTCAA GGCAATGCCG AGAGACACAC AAGATTGCAG TATTTTACGT TGCTGAAGGA
    CAAGAAGATA AATACTCCAT TCTCACCAAT ATAGGAGGAA GCCAAGCATA TGAAGATTTT
    GTAGCTGGCC TTGGTTGGGA GGTAAACCTT ACAAACCACT GTGGCTTCAT GGGAGGCCTT
    CAGAAGAACA GAAGCACTGG ACTGACCACT CCCTATTTTG CTACCTCGAC AGTAGAAGTA
    ATATTTCATG TATCTACACG AATGCCTTCT GATTCTGATG ACTCTCTGAC CAAAAAATTG
    AGACATCTAG GAAATGATGA AGTGCACATT GTTTGGTCAG AACATACTAG AGACTACAGG
    AGAGGAATTA TTCCTACTGA GTTTGGTGAT GTCCTCATTG TAATATACCC AATGAAAAAC
    CACATGTTCA GTATCCAGAT CATGAAAAAA CCAGAGGTTC CCTTCTTTGG TCCACTTTTT
    GATGGTGCTA TTGTGAATGG AAAAGTTCTG CCCATTATGG TTCGATCCAC AGCAATAAAT
    GCTAGCCGTG CTCTGAAATC ACTGATTCCA TTGTATCAAA ATTTCTATGA GGAGAGAGCA
    CGGTACCTGC AGACAATTGT CCAGCACCAT TTAGAGCCAA CAACATTTGA AGACTTTGCA
    GCACAGGTTT TCTCTCCAGC TCCCTACCAT CATTTCCCTG CTGACGCAGT TGGATCCTAC
    CCAGAGATTC CACCCAGTGA CGCTGCCCCA GCAGCACAGG TAGACGGGGC TGACCTGGCC
    TCTCCCATGT CTCCTCGAAC TAGCAAAAGC CGCATGTCCA TGAAGCTGCG TCGGTCCTCT
    GGCTCAGCCA ATAAATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86115
    Clone ID Related Accession (Same CDS sequence) XM_030246826.1
    Accession Version XM_030246826.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGGTGCAGTT
    CCTGTCGTTG ACAGTTCATC CCGTCATGCT CCAAGCTTGC AGAGTTCCAC AGAGGCTTCG
    TCAATAACTA GATCCACTGA GAGCCACCTC ACTGATACTC ATAGTAGAGA GTCTTCTCTG
    GAAGTTGGTG ATAGCATATA TGACCATCTT TGCCATTTGA TAGGTCCAGT AGAACTTGCA
    GATGCAGCTT TGGATCAAAT CCAGTACATT GACCTTGAAG GAGACGATGA CCTTCTTTCT
    TCTCTGAAAG AATACTTTAA GGAAAACCAG GAAAGTCGTA GTAAAAGTGA GCTAGGGAAA
    GATACAGCTT CCCAGGAAGT GCTTATTGAC GTAACTAGAG AGGAAATACC ATCTTTCCAT
    AAAGTAGAGT ACACAGGTCA GGAAGTAAAA CCAGAAAATC TCATGTCTTT TGTTGGGACT
    TCTGAAAATA CACCATTTCA AAAAGAACCA AGCTCAGCTG TCTTCACAAG TACTCTTGCA
    CCTAACCATT CAGATAGCTA TCCAAGATCA CAAACTGCTG AAAAGGCTAA GCAACTAAGT
    CTTGATGAAC ACTCATCGGA TCCTAGCTCA GATAGCCTAT GTGATAAAGA AAAAAAGAAA
    CATCTGTGTA GACAAACAGC CACAGAAGTA GATGCCTGTG TAGATATGAC AGTAGCTGAA
    AAGCTTAAGA GTGTACAGGT TAATGAAAAA ATCACGCCAT ACTTAATGCA TGCCAAGAAA
    ACTGCATTAA AAACACCGGT AAGCCGCAGA ATGCCTCATG TTTCAAACAC TAGCAAGCTT
    TCTCCAACTA AGAGGAGCTT GTCTGAAACA GTAACTCATA GGGCCAAAAT CATGAAAATT
    GCCGCTAAGA AACGAAATAG TGTTCATGTT ACTTTTAGAC CATCTACCGA GTCTGTCCAA
    TTTTATAATC CTCTGGAAAA CAAAGAGGCT CCATGGAAGA TGAGACTACG GAAGCTCAGT
    GGCTTTAGCA GTAATAGCAG TAGCACCACC AGCAACACAC AGACCAGCTC AACTAGTGGT
    ACAGACCTTG TAAAGCCTGG TGTGTACAGA CCTCTAGATA CAATCGGTGC AGCATCAGTT
    AGCAGAGCAG TGAGGGAATC CACAGAGATC CCCACAGCCA CATCACAGAA AGAAGGAATG
    GCAAGTAACC AGGCAGGTAG TCGCAGTACT CTCAGGTCAT CGAGTCAGGA GACAGGACCA
    CAGCAGGGCT CCCTGGGTGG CGTTTATAAA ACTGTTGTCC ATGCTCTTTC GAAGCCGAAG
    GCAAATGTTT CCCCACAGAG GCAGAACAGA ATGCCACCCG AGGCTCCACT GAGGGATCTG
    TACAGTCATG TAATGGGCTA TTTTGGAAGG AAAGCTGCAG TAAATAAAGA GGACACGAGC
    CCCAAACTGC CTCCTCTTAA TAGTGAGACT GGTGGCAACA GCGCCAATGT TCCTGACCTG
    ATGGATGAGT TTATCGCAGA GCGACTTCGA AGTGGCAATG CCTCGACTAT GACAAGAAGG
    GGGAGTAGTC CAGGCAGCCT TGAAATCCCG AAAGACCTCC CTGATATTCT AAACAAGCAG
    AACCAGATGC GGCCTGTTGA TGACCCTGGT GTACCCTCCG AATGGACGTC TCCAGCCAGT
    GCAGGGAGCA GTGATCTCAT GAGCTCAGAT AGTCACTCGG ATTCCTTCAG TGCCTTCCAG
    TGTGAGGGAC GAAAATTTGA CAACTTTGGC TTCGGGACTG ACATTGGGAT CCCATCTTCT
    GCTGATGTGG ATTTAGGTTC TGGCCATCAT CAAAGCACTG AGGAGCAGGA AGTGGCCAGC
    CTAACTACGC TTCATTTAGA TTCAGAAACA AGCAGCCTTA ATCAGCAAGC CTTCTCTGCA
    GAAGTTGCAA CCGTTACTGG TTCAGAAAGT GCTTCCCCCG TTCATTCAGC TCTGGGATCC
    AGGTCGCAGA CCCCCTCCCC ATCTACACTG AGCAGAGCTC ACATAGAACA GAAGGACCTA
    CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACGC CGGATGACCT AGAAATCAGT
    GAATTTCCAT CTGAATGTTG TAGCGTGATG GCAGGAGGTA CTCTCACTGG GTGGCATGCT
    GATGTTGCTA CTGTTATGTG GCGCAGAATG CTGGGCATTT TGGGAGATGT CAATGCTATT
    ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA
    GCCAAGATCA GAGATAACCT TGGCATTTCA GCTGACAACC TGACTTCCCC TTCTCCACCC
    GTCCTGATTC CTCCACTAAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTGACT
    GATAAGTATA AACAAGGTAA ATTACATGCA TATAAACTTA TTTGTAATAC AATGAAGAGA
    AGACAAGATG TATCTCCAAA TAGAGATTTT TTAACACATT TCTACAATAT AATGCATTGT
    GGATTGCTTC ATATTGACCA GGACATTGTC AATACGATCA TCAAGCACTG CTCACCTCAG
    TTTTTTTCAC TCGGTTTGCC TGGGGCCACG ATGCTTATTA TGGATTTTAT TATAGCAGCT
    GGGAGAGTGG CTTCTTCGGC TTTTCTTAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG
    GGATCTTTAG TTTGCTTTCC CAACTTATAT TGTGAATTGC CTGCTCTCCA TCCCAACATT
    CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATTAA AACTGTATTA
    AGCTCGGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT
    ATTTGGATTT GTGAAGAATT AGTCCACGAA TCTCATCATC CTCAAATTAA AGAAGCACTG
    AATGTAATTT GTGTTTCATT AAAGTTTACT AATAAAACAG TTGCCCACGT CGCCTGTAAC
    ATGCTTCACA TGCTGGTTCA CTATGTCCCT AGACTTCAGA TTCACCAGCC GCAGTCTCCC
    CTGAAAATTA TTCAGATCCT AATAGCAACT ATCACCCACC TTTTGCCAAG TACAGAAGCT
    TCATCATATG AAATGGACAA GAGGTTGGTT GTCTCATTAC TTCTTTGCCT TCTGGACTGG
    ATCATGGCCT TACCTTTAAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT
    GATAAGACCG AAAAGTCTGT TCTCAACTGC ATTTATAAGG TATTACATGG GTGTGTCTAT
    GGAGCGCAAA GTTTTAGCAA TCCCAAGTAC TTTCCAATAA GCCTTTCTGA TTTGGCATCT
    GTAGATTATG ATCCGTTTAT GCATTTGGAA AGTCTGAAAG AACCTGAACC TTTACACTCT
    CCAGATTCAG AGCGGTCTTC TAAACTGCAG CCTGTGACAG AAGTGAAAAC TCAGATGCAA
    CAAGGATTAA TCTCTATAGC AGCCCGTACT GTTATTACCC ATCTGGTGAA TCACTTGGGC
    CATTATCCAA TGAGTGGTGG TCCTGCTATG CTAACAAGTC AGGTGTGTGA AAATCATGAC
    AATCATTACA GTGAAAGTAC TGAACTTTCT CCTGAGCTCT TTGAAAGTCC AAACATCCAG
    TTCTTTGTGC TGAATAATAC AACCTTAGTG TCCTGTATCC AGATAAGATC GGAAGAGAGT
    ATGCCTGGAG GAGGTCTAGC TGCTGGCCTT GTGTCAGCCA ACTCAAATGT TAGAATCATA
    GTACGTGATC TCTCTGGAAA GTACTCATGG GATTCTGCCA TACTGTATGG ACCACCCATT
    GTAAGTGGTT TGCCTGAACC TACATCTTTC ATACTTTCAA TGTCTGACCA AGAGAAACCG
    GAAGAGCCTC CTACATCTAA CGAATGCTTA GAAGACATCG CAGTAAAAGA TGGGCTTTCT
    CTCCAGCTTA GAAGATTTAG AGAAACTGTA CCAACTTGGT CTACGATACG AGAGGAAGAA
    GATGTTCTTG ACGAGCTCCT GCAGTATTTA GGCACTACAA GTCCTGAGTG TTTACAGAGA
    ACGGGAATCT CCCTCAATGT TCCTGCTCCA CAGCCTCTGT GCATTTCTGA AAAACAGGAG
    AATGATGTCA TTAATGCTAT CCTTAAGCAA TACACGGAAG AAAAAGAGTT TGTAGAGAAG
    CACTTTAATG ACTTAAACAT GAAAGCCTCA GAACAAGATG AGCCAACACC TCAGAAACCT
    CAGTCAGCTT TTTATTACTG CAGATTGCTT CTTAGTATAT TGGGAATGAA TTCCTGGGAC
    AAACGGAGGA GCTTTCATCT CTTGAAGAAA AATGAAAAGC TGCTCAGAGA ACTTAGGAAC
    TTGGATTCAA GGCAATGCCG AGAGACACAC AAGATTGCAG TATTTTACGT TGCTGAAGGA
    CAAGAAGATA AATACTCCAT TCTCACCAAT ATAGGAGGAA GCCAAGCATA TGAAGATTTT
    GTAGCTGGCC TTGGTTGGGA GGTAAACCTT ACAAACCACT GTGGCTTCAT GGGAGGCCTT
    CAGAAGAACA GAAGCACTGG ACTGACCACT CCCTATTTTG CTACCTCGAC AGTAGAAGTA
    ATATTTCATG TATCTACACG AATGCCTTCT GATTCTGATG ACTCTCTGAC CAAAAAATTG
    AGACATCTAG GAAATGATGA AGTGCACATT GTTTGGTCAG AACATACTAG AGACTACAGG
    AGAGGAATTA TTCCTACTGA GTTTGGTGAT GTCCTCATTG TAATATACCC AATGAAAAAC
    CACATGTTCA GTATCCAGAT CATGAAAAAA CCAGAGGTTC CCTTCTTTGG TCCACTTTTT
    GATGGTGCTA TTGTGAATGG AAAAGTTCTG CCCATTATGG TTCGATCCAC AGCAATAAAT
    GCTAGCCGTG CTCTGAAATC ACTGATTCCA TTGTATCAAA ATTTCTATGA GGAGAGAGCA
    CGGTACCTGC AGACAATTGT CCAGCACCAT TTAGAGCCAA CAACATTTGA AGACTTTGCA
    GCACAGGTTT TCTCTCCAGC TCCCTACCAT CATTTCCCTG CTGACGCAGG ATCCTACCCA
    GAGATTCCAC CCAGTGACGC TGCCCCAGCA GCACAGGTAG ACGGGGCTGA CCTGGCCTCT
    CCCATGTCTC CTCGAACTAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG GTCCTCTGGC
    TCAGCCAATA AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102686.1
    CDS808..8565
    Translation

    Target ORF information:

    RefSeq Version XM_030246826.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246826.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGGTGCAGTT
    CCTGTCGTTG ACAGTTCATC CCGTCATGCT CCAAGCTTGC AGAGTTCCAC AGAGGCTTCG
    TCAATAACTA GATCCACTGA GAGCCACCTC ACTGATACTC ATAGTAGAGA GTCTTCTCTG
    GAAGTTGGTG ATAGCATATA TGACCATCTT TGCCATTTGA TAGGTCCAGT AGAACTTGCA
    GATGCAGCTT TGGATCAAAT CCAGTACATT GACCTTGAAG GAGACGATGA CCTTCTTTCT
    TCTCTGAAAG AATACTTTAA GGAAAACCAG GAAAGTCGTA GTAAAAGTGA GCTAGGGAAA
    GATACAGCTT CCCAGGAAGT GCTTATTGAC GTAACTAGAG AGGAAATACC ATCTTTCCAT
    AAAGTAGAGT ACACAGGTCA GGAAGTAAAA CCAGAAAATC TCATGTCTTT TGTTGGGACT
    TCTGAAAATA CACCATTTCA AAAAGAACCA AGCTCAGCTG TCTTCACAAG TACTCTTGCA
    CCTAACCATT CAGATAGCTA TCCAAGATCA CAAACTGCTG AAAAGGCTAA GCAACTAAGT
    CTTGATGAAC ACTCATCGGA TCCTAGCTCA GATAGCCTAT GTGATAAAGA AAAAAAGAAA
    CATCTGTGTA GACAAACAGC CACAGAAGTA GATGCCTGTG TAGATATGAC AGTAGCTGAA
    AAGCTTAAGA GTGTACAGGT TAATGAAAAA ATCACGCCAT ACTTAATGCA TGCCAAGAAA
    ACTGCATTAA AAACACCGGT AAGCCGCAGA ATGCCTCATG TTTCAAACAC TAGCAAGCTT
    TCTCCAACTA AGAGGAGCTT GTCTGAAACA GTAACTCATA GGGCCAAAAT CATGAAAATT
    GCCGCTAAGA AACGAAATAG TGTTCATGTT ACTTTTAGAC CATCTACCGA GTCTGTCCAA
    TTTTATAATC CTCTGGAAAA CAAAGAGGCT CCATGGAAGA TGAGACTACG GAAGCTCAGT
    GGCTTTAGCA GTAATAGCAG TAGCACCACC AGCAACACAC AGACCAGCTC AACTAGTGGT
    ACAGACCTTG TAAAGCCTGG TGTGTACAGA CCTCTAGATA CAATCGGTGC AGCATCAGTT
    AGCAGAGCAG TGAGGGAATC CACAGAGATC CCCACAGCCA CATCACAGAA AGAAGGAATG
    GCAAGTAACC AGGCAGGTAG TCGCAGTACT CTCAGGTCAT CGAGTCAGGA GACAGGACCA
    CAGCAGGGCT CCCTGGGTGG CGTTTATAAA ACTGTTGTCC ATGCTCTTTC GAAGCCGAAG
    GCAAATGTTT CCCCACAGAG GCAGAACAGA ATGCCACCCG AGGCTCCACT GAGGGATCTG
    TACAGTCATG TAATGGGCTA TTTTGGAAGG AAAGCTGCAG TAAATAAAGA GGACACGAGC
    CCCAAACTGC CTCCTCTTAA TAGTGAGACT GGTGGCAACA GCGCCAATGT TCCTGACCTG
    ATGGATGAGT TTATCGCAGA GCGACTTCGA AGTGGCAATG CCTCGACTAT GACAAGAAGG
    GGGAGTAGTC CAGGCAGCCT TGAAATCCCG AAAGACCTCC CTGATATTCT AAACAAGCAG
    AACCAGATGC GGCCTGTTGA TGACCCTGGT GTACCCTCCG AATGGACGTC TCCAGCCAGT
    GCAGGGAGCA GTGATCTCAT GAGCTCAGAT AGTCACTCGG ATTCCTTCAG TGCCTTCCAG
    TGTGAGGGAC GAAAATTTGA CAACTTTGGC TTCGGGACTG ACATTGGGAT CCCATCTTCT
    GCTGATGTGG ATTTAGGTTC TGGCCATCAT CAAAGCACTG AGGAGCAGGA AGTGGCCAGC
    CTAACTACGC TTCATTTAGA TTCAGAAACA AGCAGCCTTA ATCAGCAAGC CTTCTCTGCA
    GAAGTTGCAA CCGTTACTGG TTCAGAAAGT GCTTCCCCCG TTCATTCAGC TCTGGGATCC
    AGGTCGCAGA CCCCCTCCCC ATCTACACTG AGCAGAGCTC ACATAGAACA GAAGGACCTA
    CAGCTCGATG AGAAGCTCCA CCACTCTGTT CTTCAGACGC CGGATGACCT AGAAATCAGT
    GAATTTCCAT CTGAATGTTG TAGCGTGATG GCAGGAGGTA CTCTCACTGG GTGGCATGCT
    GATGTTGCTA CTGTTATGTG GCGCAGAATG CTGGGCATTT TGGGAGATGT CAATGCTATT
    ATGGATCCTG AAATACATGC TCAAGTTTTT GATTACCTCT GTGAACTTTG GCAGAATCTA
    GCCAAGATCA GAGATAACCT TGGCATTTCA GCTGACAACC TGACTTCCCC TTCTCCACCC
    GTCCTGATTC CTCCACTAAG AATTCTTACA CCTTGGCTTT TTAAGGCAAC CATGTTGACT
    GATAAGTATA AACAAGGTAA ATTACATGCA TATAAACTTA TTTGTAATAC AATGAAGAGA
    AGACAAGATG TATCTCCAAA TAGAGATTTT TTAACACATT TCTACAATAT AATGCATTGT
    GGATTGCTTC ATATTGACCA GGACATTGTC AATACGATCA TCAAGCACTG CTCACCTCAG
    TTTTTTTCAC TCGGTTTGCC TGGGGCCACG ATGCTTATTA TGGATTTTAT TATAGCAGCT
    GGGAGAGTGG CTTCTTCGGC TTTTCTTAAT GCACCAAGAG TGGAAGCACA AGTTCTTCTG
    GGATCTTTAG TTTGCTTTCC CAACTTATAT TGTGAATTGC CTGCTCTCCA TCCCAACATT
    CCTGATATTG CTGTGTCTCA ATTTACAGAT GTTAAGGAAC TTATAATTAA AACTGTATTA
    AGCTCGGCAA GAGATGAGCC CTCTGGTCCT GCACGATGTG TAGCACTTTG CAGTTTAGGT
    ATTTGGATTT GTGAAGAATT AGTCCACGAA TCTCATCATC CTCAAATTAA AGAAGCACTG
    AATGTAATTT GTGTTTCATT AAAGTTTACT AATAAAACAG TTGCCCACGT CGCCTGTAAC
    ATGCTTCACA TGCTGGTTCA CTATGTCCCT AGACTTCAGA TTCACCAGCC GCAGTCTCCC
    CTGAAAATTA TTCAGATCCT AATAGCAACT ATCACCCACC TTTTGCCAAG TACAGAAGCT
    TCATCATATG AAATGGACAA GAGGTTGGTT GTCTCATTAC TTCTTTGCCT TCTGGACTGG
    ATCATGGCCT TACCTTTAAA GACACTGCTC CAACCAGTCC ATGCTACAGG AGCAGAAAAT
    GATAAGACCG AAAAGTCTGT TCTCAACTGC ATTTATAAGG TATTACATGG GTGTGTCTAT
    GGAGCGCAAA GTTTTAGCAA TCCCAAGTAC TTTCCAATAA GCCTTTCTGA TTTGGCATCT
    GTAGATTATG ATCCGTTTAT GCATTTGGAA AGTCTGAAAG AACCTGAACC TTTACACTCT
    CCAGATTCAG AGCGGTCTTC TAAACTGCAG CCTGTGACAG AAGTGAAAAC TCAGATGCAA
    CAAGGATTAA TCTCTATAGC AGCCCGTACT GTTATTACCC ATCTGGTGAA TCACTTGGGC
    CATTATCCAA TGAGTGGTGG TCCTGCTATG CTAACAAGTC AGGTGTGTGA AAATCATGAC
    AATCATTACA GTGAAAGTAC TGAACTTTCT CCTGAGCTCT TTGAAAGTCC AAACATCCAG
    TTCTTTGTGC TGAATAATAC AACCTTAGTG TCCTGTATCC AGATAAGATC GGAAGAGAGT
    ATGCCTGGAG GAGGTCTAGC TGCTGGCCTT GTGTCAGCCA ACTCAAATGT TAGAATCATA
    GTACGTGATC TCTCTGGAAA GTACTCATGG GATTCTGCCA TACTGTATGG ACCACCCATT
    GTAAGTGGTT TGCCTGAACC TACATCTTTC ATACTTTCAA TGTCTGACCA AGAGAAACCG
    GAAGAGCCTC CTACATCTAA CGAATGCTTA GAAGACATCG CAGTAAAAGA TGGGCTTTCT
    CTCCAGCTTA GAAGATTTAG AGAAACTGTA CCAACTTGGT CTACGATACG AGAGGAAGAA
    GATGTTCTTG ACGAGCTCCT GCAGTATTTA GGCACTACAA GTCCTGAGTG TTTACAGAGA
    ACGGGAATCT CCCTCAATGT TCCTGCTCCA CAGCCTCTGT GCATTTCTGA AAAACAGGAG
    AATGATGTCA TTAATGCTAT CCTTAAGCAA TACACGGAAG AAAAAGAGTT TGTAGAGAAG
    CACTTTAATG ACTTAAACAT GAAAGCCTCA GAACAAGATG AGCCAACACC TCAGAAACCT
    CAGTCAGCTT TTTATTACTG CAGATTGCTT CTTAGTATAT TGGGAATGAA TTCCTGGGAC
    AAACGGAGGA GCTTTCATCT CTTGAAGAAA AATGAAAAGC TGCTCAGAGA ACTTAGGAAC
    TTGGATTCAA GGCAATGCCG AGAGACACAC AAGATTGCAG TATTTTACGT TGCTGAAGGA
    CAAGAAGATA AATACTCCAT TCTCACCAAT ATAGGAGGAA GCCAAGCATA TGAAGATTTT
    GTAGCTGGCC TTGGTTGGGA GGTAAACCTT ACAAACCACT GTGGCTTCAT GGGAGGCCTT
    CAGAAGAACA GAAGCACTGG ACTGACCACT CCCTATTTTG CTACCTCGAC AGTAGAAGTA
    ATATTTCATG TATCTACACG AATGCCTTCT GATTCTGATG ACTCTCTGAC CAAAAAATTG
    AGACATCTAG GAAATGATGA AGTGCACATT GTTTGGTCAG AACATACTAG AGACTACAGG
    AGAGGAATTA TTCCTACTGA GTTTGGTGAT GTCCTCATTG TAATATACCC AATGAAAAAC
    CACATGTTCA GTATCCAGAT CATGAAAAAA CCAGAGGTTC CCTTCTTTGG TCCACTTTTT
    GATGGTGCTA TTGTGAATGG AAAAGTTCTG CCCATTATGG TTCGATCCAC AGCAATAAAT
    GCTAGCCGTG CTCTGAAATC ACTGATTCCA TTGTATCAAA ATTTCTATGA GGAGAGAGCA
    CGGTACCTGC AGACAATTGT CCAGCACCAT TTAGAGCCAA CAACATTTGA AGACTTTGCA
    GCACAGGTTT TCTCTCCAGC TCCCTACCAT CATTTCCCTG CTGACGCAGG ATCCTACCCA
    GAGATTCCAC CCAGTGACGC TGCCCCAGCA GCACAGGTAG ACGGGGCTGA CCTGGCCTCT
    CCCATGTCTC CTCGAACTAG CAAAAGCCGC ATGTCCATGA AGCTGCGTCG GTCCTCTGGC
    TCAGCCAATA AATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86116
    Clone ID Related Accession (Same CDS sequence) XM_006516109.3
    Accession Version XM_006516109.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516109.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GGTGCAGTTC CTGTCGTTGA CAGTTCATCC
    CGTCATGCTC CAAGCTTGCA GAGTTCCACA GAGGCTTCGT CAATAACTAG ATCCACTGAG
    AGCCACCTCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT
    GACCATCTTT GCCATTTGAT AGGTCCAGTA GAACTTGCAG ATGCAGCTTT GGATCAAATC
    CAGTACATTG ACCTTGAAGG AGACGATGAC CTTCTTTCTT CTCTGAAAGA ATACTTTAAG
    GAAAACCAGG AAAGTCGTAG TAAAAGTGAG CTAGGGAAAG ATACAGCTTC CCAGGAAGTG
    CTTATTGACG TAACTAGAGA GGAAATACCA TCTTTCCATA AAGTAGAGTA CACAGGTCAG
    GAAGTAAAAC CAGAAAATCT CATGTCTTTT GTTGGGACTT CTGAAAATAC ACCATTTCAA
    AAAGAACCAA GCTCAGCTGT CTTCACAAGT ACTCTTGCAC CTAACCATTC AGATAGCTAT
    CCAAGATCAC AAACTGCTGA AAAGGCTAAG CAACTAAGTC TTGATGAACA CTCATCGGAT
    CCTAGCTCAG ATAGCCTATG TGATAAAGAA AAAAAGAAAC ATCTGTGTAG ACAAACAGCC
    ACAGAAGTAG ATGCCTGTGT AGATATGACA GTAGCTGAAA AGCTTAAGAG TGTACAGGTT
    AATGAAAAAA TCACGCCATA CTTAATGCAT GCCAAGAAAA CTGCATTAAA AACACCGGTA
    AGCCGCAGAA TGCCTCATGT TTCAAACACT AGCAAGCTTT CTCCAACTAA GAGGAGCTTG
    TCTGAAACAG TAACTCATAG GGCCAAAATC ATGAAAATTG CCGCTAAGAA ACGAAATAGT
    GTTCATGTTA CTTTTAGACC ATCTACCGAG TCTGTCCAAT TTTATAATCC TCTGGAAAAC
    AAAGAGGCTC CATGGAAGAT GAGACTACGG AAGCTCAGTG GCTTTAGCAG TAATAGCAGT
    AGCACCACCA GCAACACACA GACCAGCTCA ACTAGTGGTA CAGACCTTGT AAAGCCTGGT
    GTGTACAGAC CTCTAGATAC AATCGGTGCA GCATCAGTTA GCAGAGCAGT GAGGGAATCC
    ACAGAGATCC CCACAGCCAC ATCACAGAAA GAAGGAATGG CAAGTAACCA GGCAGGTAGT
    CGCAGTACTC TCAGGTCATC GAGTCAGGAG ACAGGACCAC AGCAGGGCTC CCTGGGTGGC
    GTTTATAAAA CTGTTGTCCA TGCTCTTTCG AAGCCGAAGG CAAATGTTTC CCCACAGAGG
    CAGAACAGAA TGCCACCCGA GGCTCCACTG AGGGATCTGT ACAGTCATGT AATGGGCTAT
    TTTGGAAGGA AAGCTGCAGT AAATAAAGAG GACACGAGCC CCAAACTGCC TCCTCTTAAT
    AGTGAGACTG GTGGCAACAG CGCCAATGTT CCTGACCTGA TGGATGAGTT TATCGCAGAG
    CGACTTCGAA GTGGCAATGC CTCGACTATG ACAAGAAGGG GGAGTAGTCC AGGCAGCCTT
    GAAATCCCGA AAGACCTCCC TGATATTCTA AACAAGCAGA ACCAGATGCG GCCTGTTGAT
    GACCCTGGTG TACCCTCCGA ATGGACGTCT CCAGCCAGTG CAGGGAGCAG TGATCTCATG
    AGCTCAGATA GTCACTCGGA TTCCTTCAGT GCCTTCCAGT GTGAGGGACG AAAATTTGAC
    AACTTTGGCT TCGGGACTGA CATTGGGATC CCATCTTCTG CTGATGTGGA TTTAGGTTCT
    GGCCATCATC AAAGCACTGA GGAGCAGGAA GTGGCCAGCC TAACTACGCT TCATTTAGAT
    TCAGAAACAA GCAGCCTTAA TCAGCAAGCC TTCTCTGCAG AAGTTGCAAC CGTTACTGGT
    TCAGAAAGTG CTTCCCCCGT TCATTCAGCT CTGGGATCCA GGTCGCAGAC CCCCTCCCCA
    TCTACACTGA GCAGAGCTCA CATAGAACAG AAGGACCTAC AGCTCGATGA GAAGCTCCAC
    CACTCTGTTC TTCAGACGCC GGATGACCTA GAAATCAGTG AATTTCCATC TGAATGTTGT
    AGCGTGATGG CAGGAGGTAC TCTCACTGGG TGGCATGCTG ATGTTGCTAC TGTTATGTGG
    CGCAGAATGC TGGGCATTTT GGGAGATGTC AATGCTATTA TGGATCCTGA AATACATGCT
    CAAGTTTTTG ATTACCTCTG TGAACTTTGG CAGAATCTAG CCAAGATCAG AGATAACCTT
    GGCATTTCAG CTGACAACCT GACTTCCCCT TCTCCACCCG TCCTGATTCC TCCACTAAGA
    ATTCTTACAC CTTGGCTTTT TAAGGCAACC ATGTTGACTG ATAAGTATAA ACAAGGTAAA
    TTACATGCAT ATAAACTTAT TTGTAATACA ATGAAGAGAA GACAAGATGT ATCTCCAAAT
    AGAGATTTTT TAACACATTT CTACAATATA ATGCATTGTG GATTGCTTCA TATTGACCAG
    GACATTGTCA ATACGATCAT CAAGCACTGC TCACCTCAGT TTTTTTCACT CGGTTTGCCT
    GGGGCCACGA TGCTTATTAT GGATTTTATT ATAGCAGCTG GGAGAGTGGC TTCTTCGGCT
    TTTCTTAATG CACCAAGAGT GGAAGCACAA GTTCTTCTGG GATCTTTAGT TTGCTTTCCC
    AACTTATATT GTGAATTGCC TGCTCTCCAT CCCAACATTC CTGATATTGC TGTGTCTCAA
    TTTACAGATG TTAAGGAACT TATAATTAAA ACTGTATTAA GCTCGGCAAG AGATGAGCCC
    TCTGGTCCTG CACGATGTGT AGCACTTTGC AGTTTAGGTA TTTGGATTTG TGAAGAATTA
    GTCCACGAAT CTCATCATCC TCAAATTAAA GAAGCACTGA ATGTAATTTG TGTTTCATTA
    AAGTTTACTA ATAAAACAGT TGCCCACGTC GCCTGTAACA TGCTTCACAT GCTGGTTCAC
    TATGTCCCTA GACTTCAGAT TCACCAGCCG CAGTCTCCCC TGAAAATTAT TCAGATCCTA
    ATAGCAACTA TCACCCACCT TTTGCCAAGT ACAGAAGCTT CATCATATGA AATGGACAAG
    AGGTTGGTTG TCTCATTACT TCTTTGCCTT CTGGACTGGA TCATGGCCTT ACCTTTAAAG
    ACACTGCTCC AACCAGTCCA TGCTACAGGA GCAGAAAATG ATAAGACCGA AAAGTCTGTT
    CTCAACTGCA TTTATAAGGT ATTACATGGG TGTGTCTATG GAGCGCAAAG TTTTAGCAAT
    CCCAAGTACT TTCCAATAAG CCTTTCTGAT TTGGCATCTG TAGATTATGA TCCGTTTATG
    CATTTGGAAA GTCTGAAAGA ACCTGAACCT TTACACTCTC CAGATTCAGA GCGGTCTTCT
    AAACTGCAGC CTGTGACAGA AGTGAAAACT CAGATGCAAC AAGGATTAAT CTCTATAGCA
    GCCCGTACTG TTATTACCCA TCTGGTGAAT CACTTGGGCC ATTATCCAAT GAGTGGTGGT
    CCTGCTATGC TAACAAGTCA GGTGTGTGAA AATCATGACA ATCATTACAG TGAAAGTACT
    GAACTTTCTC CTGAGCTCTT TGAAAGTCCA AACATCCAGT TCTTTGTGCT GAATAATACA
    ACCTTAGTGT CCTGTATCCA GATAAGATCG GAAGAGAGTA TGCCTGGAGG AGGTCTAGCT
    GCTGGCCTTG TGTCAGCCAA CTCAAATGTT AGAATCATAG TACGTGATCT CTCTGGAAAG
    TACTCATGGG ATTCTGCCAT ACTGTATGGA CCACCCATTG TAAGTGGTTT GCCTGAACCT
    ACATCTTTCA TACTTTCAAT GTCTGACCAA GAGAAACCGG AAGAGCCTCC TACATCTAAC
    GAATGCTTAG AAGACATCGC AGTAAAAGAT GGGCTTTCTC TCCAGCTTAG AAGATTTAGA
    GAAACTGTAC CAACTTGGTC TACGATACGA GAGGAAGAAG ATGTTCTTGA CGAGCTCCTG
    CAGTATTTAG GCACTACAAG TCCTGAGTGT TTACAGAGAA CGGGAATCTC CCTCAATGTT
    CCTGCTCCAC AGCCTCTGTG CATTTCTGAA AAACAGGAGA ATGATGTCAT TAATGCTATC
    CTTAAGCAAT ACACGGAAGA AAAAGAGTTT GTAGAGAAGC ACTTTAATGA CTTAAACATG
    AAAGCCTCAG AACAAGATGA GCCAACACCT CAGAAACCTC AGTCAGCTTT TTATTACTGC
    AGATTGCTTC TTAGTATATT GGGAATGAAT TCCTGGGACA AACGGAGGAG CTTTCATCTC
    TTGAAGAAAA ATGAAAAGCT GCTCAGAGAA CTTAGGAACT TGGATTCAAG GCAATGCCGA
    GAGACACACA AGATTGCAGT ATTTTACGTT GCTGAAGGAC AAGAAGATAA ATACTCCATT
    CTCACCAATA TAGGAGGAAG CCAAGCATAT GAAGATTTTG TAGCTGGCCT TGGTTGGGAG
    GTAAACCTTA CAAACCACTG TGGCTTCATG GGAGGCCTTC AGAAGAACAG AAGCACTGGA
    CTGACCACTC CCTATTTTGC TACCTCGACA GTAGAAGTAA TATTTCATGT ATCTACACGA
    ATGCCTTCTG ATTCTGATGA CTCTCTGACC AAAAAATTGA GACATCTAGG AAATGATGAA
    GTGCACATTG TTTGGTCAGA ACATACTAGA GACTACAGGA GAGGAATTAT TCCTACTGAG
    TTTGGTGATG TCCTCATTGT AATATACCCA ATGAAAAACC ACATGTTCAG TATCCAGATC
    ATGAAAAAAC CAGAGGTTCC CTTCTTTGGT CCACTTTTTG ATGGTGCTAT TGTGAATGGA
    AAAGTTCTGC CCATTATGGT TCGATCCACA GCAATAAATG CTAGCCGTGC TCTGAAATCA
    CTGATTCCAT TGTATCAAAA TTTCTATGAG GAGAGAGCAC GGTACCTGCA GACAATTGTC
    CAGCACCATT TAGAGCCAAC AACATTTGAA GACTTTGCAG CACAGGTTTT CTCTCCAGCT
    CCCTACCATC ATTTCCCTGC TGACGCAGTT GGATCCTACC CAGAGATTCC ACCCAGTGAC
    GCTGCCCCAG CAGCACAGGT AGACGGGGCT GACCTGGCCT CTCCCATGTC TCCTCGAACT
    AGCAAAAGCC GCATGTCCAT GAAGCTGCGT CGGTCCTCTG GCTCAGCCAA TAAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516172.3
    CDS808..8427
    Translation

    Target ORF information:

    RefSeq Version XM_006516109.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516109.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GGTGCAGTTC CTGTCGTTGA CAGTTCATCC
    CGTCATGCTC CAAGCTTGCA GAGTTCCACA GAGGCTTCGT CAATAACTAG ATCCACTGAG
    AGCCACCTCA CTGATACTCA TAGTAGAGAG TCTTCTCTGG AAGTTGGTGA TAGCATATAT
    GACCATCTTT GCCATTTGAT AGGTCCAGTA GAACTTGCAG ATGCAGCTTT GGATCAAATC
    CAGTACATTG ACCTTGAAGG AGACGATGAC CTTCTTTCTT CTCTGAAAGA ATACTTTAAG
    GAAAACCAGG AAAGTCGTAG TAAAAGTGAG CTAGGGAAAG ATACAGCTTC CCAGGAAGTG
    CTTATTGACG TAACTAGAGA GGAAATACCA TCTTTCCATA AAGTAGAGTA CACAGGTCAG
    GAAGTAAAAC CAGAAAATCT CATGTCTTTT GTTGGGACTT CTGAAAATAC ACCATTTCAA
    AAAGAACCAA GCTCAGCTGT CTTCACAAGT ACTCTTGCAC CTAACCATTC AGATAGCTAT
    CCAAGATCAC AAACTGCTGA AAAGGCTAAG CAACTAAGTC TTGATGAACA CTCATCGGAT
    CCTAGCTCAG ATAGCCTATG TGATAAAGAA AAAAAGAAAC ATCTGTGTAG ACAAACAGCC
    ACAGAAGTAG ATGCCTGTGT AGATATGACA GTAGCTGAAA AGCTTAAGAG TGTACAGGTT
    AATGAAAAAA TCACGCCATA CTTAATGCAT GCCAAGAAAA CTGCATTAAA AACACCGGTA
    AGCCGCAGAA TGCCTCATGT TTCAAACACT AGCAAGCTTT CTCCAACTAA GAGGAGCTTG
    TCTGAAACAG TAACTCATAG GGCCAAAATC ATGAAAATTG CCGCTAAGAA ACGAAATAGT
    GTTCATGTTA CTTTTAGACC ATCTACCGAG TCTGTCCAAT TTTATAATCC TCTGGAAAAC
    AAAGAGGCTC CATGGAAGAT GAGACTACGG AAGCTCAGTG GCTTTAGCAG TAATAGCAGT
    AGCACCACCA GCAACACACA GACCAGCTCA ACTAGTGGTA CAGACCTTGT AAAGCCTGGT
    GTGTACAGAC CTCTAGATAC AATCGGTGCA GCATCAGTTA GCAGAGCAGT GAGGGAATCC
    ACAGAGATCC CCACAGCCAC ATCACAGAAA GAAGGAATGG CAAGTAACCA GGCAGGTAGT
    CGCAGTACTC TCAGGTCATC GAGTCAGGAG ACAGGACCAC AGCAGGGCTC CCTGGGTGGC
    GTTTATAAAA CTGTTGTCCA TGCTCTTTCG AAGCCGAAGG CAAATGTTTC CCCACAGAGG
    CAGAACAGAA TGCCACCCGA GGCTCCACTG AGGGATCTGT ACAGTCATGT AATGGGCTAT
    TTTGGAAGGA AAGCTGCAGT AAATAAAGAG GACACGAGCC CCAAACTGCC TCCTCTTAAT
    AGTGAGACTG GTGGCAACAG CGCCAATGTT CCTGACCTGA TGGATGAGTT TATCGCAGAG
    CGACTTCGAA GTGGCAATGC CTCGACTATG ACAAGAAGGG GGAGTAGTCC AGGCAGCCTT
    GAAATCCCGA AAGACCTCCC TGATATTCTA AACAAGCAGA ACCAGATGCG GCCTGTTGAT
    GACCCTGGTG TACCCTCCGA ATGGACGTCT CCAGCCAGTG CAGGGAGCAG TGATCTCATG
    AGCTCAGATA GTCACTCGGA TTCCTTCAGT GCCTTCCAGT GTGAGGGACG AAAATTTGAC
    AACTTTGGCT TCGGGACTGA CATTGGGATC CCATCTTCTG CTGATGTGGA TTTAGGTTCT
    GGCCATCATC AAAGCACTGA GGAGCAGGAA GTGGCCAGCC TAACTACGCT TCATTTAGAT
    TCAGAAACAA GCAGCCTTAA TCAGCAAGCC TTCTCTGCAG AAGTTGCAAC CGTTACTGGT
    TCAGAAAGTG CTTCCCCCGT TCATTCAGCT CTGGGATCCA GGTCGCAGAC CCCCTCCCCA
    TCTACACTGA GCAGAGCTCA CATAGAACAG AAGGACCTAC AGCTCGATGA GAAGCTCCAC
    CACTCTGTTC TTCAGACGCC GGATGACCTA GAAATCAGTG AATTTCCATC TGAATGTTGT
    AGCGTGATGG CAGGAGGTAC TCTCACTGGG TGGCATGCTG ATGTTGCTAC TGTTATGTGG
    CGCAGAATGC TGGGCATTTT GGGAGATGTC AATGCTATTA TGGATCCTGA AATACATGCT
    CAAGTTTTTG ATTACCTCTG TGAACTTTGG CAGAATCTAG CCAAGATCAG AGATAACCTT
    GGCATTTCAG CTGACAACCT GACTTCCCCT TCTCCACCCG TCCTGATTCC TCCACTAAGA
    ATTCTTACAC CTTGGCTTTT TAAGGCAACC ATGTTGACTG ATAAGTATAA ACAAGGTAAA
    TTACATGCAT ATAAACTTAT TTGTAATACA ATGAAGAGAA GACAAGATGT ATCTCCAAAT
    AGAGATTTTT TAACACATTT CTACAATATA ATGCATTGTG GATTGCTTCA TATTGACCAG
    GACATTGTCA ATACGATCAT CAAGCACTGC TCACCTCAGT TTTTTTCACT CGGTTTGCCT
    GGGGCCACGA TGCTTATTAT GGATTTTATT ATAGCAGCTG GGAGAGTGGC TTCTTCGGCT
    TTTCTTAATG CACCAAGAGT GGAAGCACAA GTTCTTCTGG GATCTTTAGT TTGCTTTCCC
    AACTTATATT GTGAATTGCC TGCTCTCCAT CCCAACATTC CTGATATTGC TGTGTCTCAA
    TTTACAGATG TTAAGGAACT TATAATTAAA ACTGTATTAA GCTCGGCAAG AGATGAGCCC
    TCTGGTCCTG CACGATGTGT AGCACTTTGC AGTTTAGGTA TTTGGATTTG TGAAGAATTA
    GTCCACGAAT CTCATCATCC TCAAATTAAA GAAGCACTGA ATGTAATTTG TGTTTCATTA
    AAGTTTACTA ATAAAACAGT TGCCCACGTC GCCTGTAACA TGCTTCACAT GCTGGTTCAC
    TATGTCCCTA GACTTCAGAT TCACCAGCCG CAGTCTCCCC TGAAAATTAT TCAGATCCTA
    ATAGCAACTA TCACCCACCT TTTGCCAAGT ACAGAAGCTT CATCATATGA AATGGACAAG
    AGGTTGGTTG TCTCATTACT TCTTTGCCTT CTGGACTGGA TCATGGCCTT ACCTTTAAAG
    ACACTGCTCC AACCAGTCCA TGCTACAGGA GCAGAAAATG ATAAGACCGA AAAGTCTGTT
    CTCAACTGCA TTTATAAGGT ATTACATGGG TGTGTCTATG GAGCGCAAAG TTTTAGCAAT
    CCCAAGTACT TTCCAATAAG CCTTTCTGAT TTGGCATCTG TAGATTATGA TCCGTTTATG
    CATTTGGAAA GTCTGAAAGA ACCTGAACCT TTACACTCTC CAGATTCAGA GCGGTCTTCT
    AAACTGCAGC CTGTGACAGA AGTGAAAACT CAGATGCAAC AAGGATTAAT CTCTATAGCA
    GCCCGTACTG TTATTACCCA TCTGGTGAAT CACTTGGGCC ATTATCCAAT GAGTGGTGGT
    CCTGCTATGC TAACAAGTCA GGTGTGTGAA AATCATGACA ATCATTACAG TGAAAGTACT
    GAACTTTCTC CTGAGCTCTT TGAAAGTCCA AACATCCAGT TCTTTGTGCT GAATAATACA
    ACCTTAGTGT CCTGTATCCA GATAAGATCG GAAGAGAGTA TGCCTGGAGG AGGTCTAGCT
    GCTGGCCTTG TGTCAGCCAA CTCAAATGTT AGAATCATAG TACGTGATCT CTCTGGAAAG
    TACTCATGGG ATTCTGCCAT ACTGTATGGA CCACCCATTG TAAGTGGTTT GCCTGAACCT
    ACATCTTTCA TACTTTCAAT GTCTGACCAA GAGAAACCGG AAGAGCCTCC TACATCTAAC
    GAATGCTTAG AAGACATCGC AGTAAAAGAT GGGCTTTCTC TCCAGCTTAG AAGATTTAGA
    GAAACTGTAC CAACTTGGTC TACGATACGA GAGGAAGAAG ATGTTCTTGA CGAGCTCCTG
    CAGTATTTAG GCACTACAAG TCCTGAGTGT TTACAGAGAA CGGGAATCTC CCTCAATGTT
    CCTGCTCCAC AGCCTCTGTG CATTTCTGAA AAACAGGAGA ATGATGTCAT TAATGCTATC
    CTTAAGCAAT ACACGGAAGA AAAAGAGTTT GTAGAGAAGC ACTTTAATGA CTTAAACATG
    AAAGCCTCAG AACAAGATGA GCCAACACCT CAGAAACCTC AGTCAGCTTT TTATTACTGC
    AGATTGCTTC TTAGTATATT GGGAATGAAT TCCTGGGACA AACGGAGGAG CTTTCATCTC
    TTGAAGAAAA ATGAAAAGCT GCTCAGAGAA CTTAGGAACT TGGATTCAAG GCAATGCCGA
    GAGACACACA AGATTGCAGT ATTTTACGTT GCTGAAGGAC AAGAAGATAA ATACTCCATT
    CTCACCAATA TAGGAGGAAG CCAAGCATAT GAAGATTTTG TAGCTGGCCT TGGTTGGGAG
    GTAAACCTTA CAAACCACTG TGGCTTCATG GGAGGCCTTC AGAAGAACAG AAGCACTGGA
    CTGACCACTC CCTATTTTGC TACCTCGACA GTAGAAGTAA TATTTCATGT ATCTACACGA
    ATGCCTTCTG ATTCTGATGA CTCTCTGACC AAAAAATTGA GACATCTAGG AAATGATGAA
    GTGCACATTG TTTGGTCAGA ACATACTAGA GACTACAGGA GAGGAATTAT TCCTACTGAG
    TTTGGTGATG TCCTCATTGT AATATACCCA ATGAAAAACC ACATGTTCAG TATCCAGATC
    ATGAAAAAAC CAGAGGTTCC CTTCTTTGGT CCACTTTTTG ATGGTGCTAT TGTGAATGGA
    AAAGTTCTGC CCATTATGGT TCGATCCACA GCAATAAATG CTAGCCGTGC TCTGAAATCA
    CTGATTCCAT TGTATCAAAA TTTCTATGAG GAGAGAGCAC GGTACCTGCA GACAATTGTC
    CAGCACCATT TAGAGCCAAC AACATTTGAA GACTTTGCAG CACAGGTTTT CTCTCCAGCT
    CCCTACCATC ATTTCCCTGC TGACGCAGTT GGATCCTACC CAGAGATTCC ACCCAGTGAC
    GCTGCCCCAG CAGCACAGGT AGACGGGGCT GACCTGGCCT CTCCCATGTC TCCTCGAACT
    AGCAAAAGCC GCATGTCCAT GAAGCTGCGT CGGTCCTCTG GCTCAGCCAA TAAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu04894
    Clone ID Related Accession (Same CDS sequence) NM_001112714.2 , NM_019994.5
    Accession Version NM_001112714.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE650514.1, AK153664.1, AK042116.1, AY066011.1, CN459409.1, AB032400.1 and CD776675.1. On Nov 1, 2013 this sequence version replaced NM_001112714.1. Transcript Variant: This variant (1) lacks an in-frame exon in the 5' coding region and a 3 nt exon in the 3' UTR, compared to variant 4. The resulting isoform (2) lacks an internal segment, compared to isoform 4. Variants 1 and 2 encode the same isoform 2. ##Evidence-Data-START## Transcript exon combination :: AY596973.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106185.1
    CDS750..6857
    Translation

    Target ORF information:

    RefSeq Version NM_001112714.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001112714.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu04894
    Clone ID Related Accession (Same CDS sequence) NM_001112714.2 , NM_019994.5
    Accession Version NM_019994.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE650514.1, AK153664.1, AK042116.1, AY066011.1, CN459409.1, AB032400.1, AC107633.11 and CD776675.1. On Nov 1, 2013 this sequence version replaced NM_019994.4. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 4. The resulting isoform (2) lacks an internal segment, compared to isoform 4. Variants 1 and 2 encode the same isoform 2. ##Evidence-Data-START## Transcript exon combination :: AY596972.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_064378.4
    CDS750..6857
    Translation

    Target ORF information:

    RefSeq Version NM_019994.5
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_019994.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG ATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu09221
    Clone ID Related Accession (Same CDS sequence) NM_001286263.1 , XM_006516106.2
    Accession Version NM_001286263.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE650514.1, AK153664.1, AK042116.1, AC159624.2, AY066011.1, CN459409.1, AB032400.1, AC107633.11 and CD776675.1. Transcript Variant: This variant (4) encodes isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC145119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273192.1
    CDS750..6998
    Translation

    Target ORF information:

    RefSeq Version NM_001286263.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001286263.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu10514
    Clone ID Related Accession (Same CDS sequence) NM_001003719.2
    Accession Version NM_001003719.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE650514.1, AK153664.1, AK042116.1, AY066011.1, CN459409.1, AB032400.1, AC107633.11 and CD776675.1. On Nov 1, 2013 this sequence version replaced NM_001003719.1. Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region and an internal exon in the 3' region which results in an alternate downstream termination codon, compared to variant 4. The resulting isoform (3) lacks an internal segment but has a longer and distinct C-terminus, compared to isoform 4. ##Evidence-Data-START## Transcript exon combination :: AY596974.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG TTGGATCCTA CCCAGAGATT
    CCACCCAGTG ACGCTGCCCC AGCAGCACAG GTAGACGGGG CTGACCTGGC CTCTCCCATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGGTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001003719.1
    CDS750..7001
    Translation

    Target ORF information:

    RefSeq Version NM_001003719.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001003719.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG TTGGATCCTA CCCAGAGATT
    CCACCCAGTG ACGCTGCCCC AGCAGCACAG GTAGACGGGG CTGACCTGGC CTCTCCCATG
    TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGGTCCTC TGGCTCAGCC
    AATAAATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu09221
    Clone ID Related Accession (Same CDS sequence) NM_001286263.1 , XM_006516106.2
    Accession Version XM_006516106.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006516106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516169.1
    CDS809..7057
    Translation

    Target ORF information:

    RefSeq Version XM_006516106.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516106.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48011
    Clone ID Related Accession (Same CDS sequence) XM_006516104.2 , XM_006516104.3
    Accession Version XM_006516104.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006516104.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GTTGGATCCT ACCCAGAGAT TCCACCCAGT GACGCTGCCC CAGCAGCACA GGTAGACGGG
    GCTGACCTGG CCTCTCCCAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGGTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516167.1
    CDS809..7201
    Translation

    Target ORF information:

    RefSeq Version XM_006516104.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516104.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GTTGGATCCT ACCCAGAGAT TCCACCCAGT GACGCTGCCC CAGCAGCACA GGTAGACGGG
    GCTGACCTGG CCTCTCCCAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG
    CGTCGGTCCT CTGGCTCAGC CAATAAATCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48012
    Clone ID Related Accession (Same CDS sequence) XM_006516105.2
    Accession Version XM_006516105.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006516105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GGATCCTACC CAGAGATTCC ACCCAGTGAC GCTGCCCCAG CAGCACAGGT AGACGGGGCT
    GACCTGGCCT CTCCCATGTC TCCTCGAACT AGCAAAAGCC GCATGTCCAT GAAGCTGCGT
    CGGTCCTCTG GCTCAGCCAA TAAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516168.1
    CDS809..7198
    Translation

    Target ORF information:

    RefSeq Version XM_006516105.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516105.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGCCATGAG AAGCCGATCC
    ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG
    ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT
    TCAGATATTG ATGATGCTCA AATCCTTCCC CGCTCAACCA GAGTCAGACA TTTTTCACAG
    AGTGAAGACA CTGGAAATGA AGTTTTTGGT GCTTTGCATG AGGAACAGCC ACTGCCTCGA
    AGTAGCAGCA CTTCTGACAT CTTGGAACCA TTCACTGTTG AGCGAGCCAA AGTAAATAAA
    GAGGACACGA GCCCCAAACT GCCTCCTCTT AATAGTGAGA CTGGTGGCAA CAGCGCCAAT
    GTTCCTGACC TGATGGATGA GTTTATCGCA GAGCGACTTC GAAGTGGCAA TGCCTCGACT
    ATGACAAGAA GGGGGAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT
    CTAAACAAGC AGAACCAGAT GCGGCCTGTT GATGACCCTG GTGTACCCTC CGAATGGACG
    TCTCCAGCCA GTGCAGGGAG CAGTGATCTC ATGAGCTCAG ATAGTCACTC GGATTCCTTC
    AGTGCCTTCC AGTGTGAGGG ACGAAAATTT GACAACTTTG GCTTCGGGAC TGACATTGGG
    ATCCCATCTT CTGCTGATGT GGATTTAGGT TCTGGCCATC ATCAAAGCAC TGAGGAGCAG
    GAAGTGGCCA GCCTAACTAC GCTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA
    GCCTTCTCTG CAGAAGTTGC AACCGTTACT GGTTCAGAAA GTGCTTCCCC CGTTCATTCA
    GCTCTGGGAT CCAGGTCGCA GACCCCCTCC CCATCTACAC TGAGCAGAGC TCACATAGAA
    CAGAAGGACC TACAGCTCGA TGAGAAGCTC CACCACTCTG TTCTTCAGAC GCCGGATGAC
    CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT
    GGGTGGCATG CTGATGTTGC TACTGTTATG TGGCGCAGAA TGCTGGGCAT TTTGGGAGAT
    GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTTT TTGATTACCT CTGTGAACTT
    TGGCAGAATC TAGCCAAGAT CAGAGATAAC CTTGGCATTT CAGCTGACAA CCTGACTTCC
    CCTTCTCCAC CCGTCCTGAT TCCTCCACTA AGAATTCTTA CACCTTGGCT TTTTAAGGCA
    ACCATGTTGA CTGATAAGTA TAAACAAGGT AAATTACATG CATATAAACT TATTTGTAAT
    ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TTTTAACACA TTTCTACAAT
    ATAATGCATT GTGGATTGCT TCATATTGAC CAGGACATTG TCAATACGAT CATCAAGCAC
    TGCTCACCTC AGTTTTTTTC ACTCGGTTTG CCTGGGGCCA CGATGCTTAT TATGGATTTT
    ATTATAGCAG CTGGGAGAGT GGCTTCTTCG GCTTTTCTTA ATGCACCAAG AGTGGAAGCA
    CAAGTTCTTC TGGGATCTTT AGTTTGCTTT CCCAACTTAT ATTGTGAATT GCCTGCTCTC
    CATCCCAACA TTCCTGATAT TGCTGTGTCT CAATTTACAG ATGTTAAGGA ACTTATAATT
    AAAACTGTAT TAAGCTCGGC AAGAGATGAG CCCTCTGGTC CTGCACGATG TGTAGCACTT
    TGCAGTTTAG GTATTTGGAT TTGTGAAGAA TTAGTCCACG AATCTCATCA TCCTCAAATT
    AAAGAAGCAC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAC
    GTCGCCTGTA ACATGCTTCA CATGCTGGTT CACTATGTCC CTAGACTTCA GATTCACCAG
    CCGCAGTCTC CCCTGAAAAT TATTCAGATC CTAATAGCAA CTATCACCCA CCTTTTGCCA
    AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGTTGG TTGTCTCATT ACTTCTTTGC
    CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACACTGC TCCAACCAGT CCATGCTACA
    GGAGCAGAAA ATGATAAGAC CGAAAAGTCT GTTCTCAACT GCATTTATAA GGTATTACAT
    GGGTGTGTCT ATGGAGCGCA AAGTTTTAGC AATCCCAAGT ACTTTCCAAT AAGCCTTTCT
    GATTTGGCAT CTGTAGATTA TGATCCGTTT ATGCATTTGG AAAGTCTGAA AGAACCTGAA
    CCTTTACACT CTCCAGATTC AGAGCGGTCT TCTAAACTGC AGCCTGTGAC AGAAGTGAAA
    ACTCAGATGC AACAAGGATT AATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG
    AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTAACAAG TCAGGTGTGT
    GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT
    CCAAACATCC AGTTCTTTGT GCTGAATAAT ACAACCTTAG TGTCCTGTAT CCAGATAAGA
    TCGGAAGAGA GTATGCCTGG AGGAGGTCTA GCTGCTGGCC TTGTGTCAGC CAACTCAAAT
    GTTAGAATCA TAGTACGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT
    GGACCACCCA TTGTAAGTGG TTTGCCTGAA CCTACATCTT TCATACTTTC AATGTCTGAC
    CAAGAGAAAC CGGAAGAGCC TCCTACATCT AACGAATGCT TAGAAGACAT CGCAGTAAAA
    GATGGGCTTT CTCTCCAGCT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACGATA
    CGAGAGGAAG AAGATGTTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAG
    TGTTTACAGA GAACGGGAAT CTCCCTCAAT GTTCCTGCTC CACAGCCTCT GTGCATTTCT
    GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACGGA AGAAAAAGAG
    TTTGTAGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAGCCAACA
    CCTCAGAAAC CTCAGTCAGC TTTTTATTAC TGCAGATTGC TTCTTAGTAT ATTGGGAATG
    AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCTTGAAGA AAAATGAAAA GCTGCTCAGA
    GAACTTAGGA ACTTGGATTC AAGGCAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC
    GTTGCTGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA
    TATGAAGATT TTGTAGCTGG CCTTGGTTGG GAGGTAAACC TTACAAACCA CTGTGGCTTC
    ATGGGAGGCC TTCAGAAGAA CAGAAGCACT GGACTGACCA CTCCCTATTT TGCTACCTCG
    ACAGTAGAAG TAATATTTCA TGTATCTACA CGAATGCCTT CTGATTCTGA TGACTCTCTG
    ACCAAAAAAT TGAGACATCT AGGAAATGAT GAAGTGCACA TTGTTTGGTC AGAACATACT
    AGAGACTACA GGAGAGGAAT TATTCCTACT GAGTTTGGTG ATGTCCTCAT TGTAATATAC
    CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT
    GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC
    ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCACTGATTC CATTGTATCA AAATTTCTAT
    GAGGAGAGAG CACGGTACCT GCAGACAATT GTCCAGCACC ATTTAGAGCC AACAACATTT
    GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCTCCCTACC ATCATTTCCC TGCTGACGCA
    GGATCCTACC CAGAGATTCC ACCCAGTGAC GCTGCCCCAG CAGCACAGGT AGACGGGGCT
    GACCTGGCCT CTCCCATGTC TCCTCGAACT AGCAAAAGCC GCATGTCCAT GAAGCTGCGT
    CGGTCCTCTG GCTCAGCCAA TAAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48013
    Clone ID Related Accession (Same CDS sequence) XM_006516107.2 , XM_006516107.3
    Accession Version XM_006516107.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ral GTPase-activating protein subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006516107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG GATCCTACCC AGAGATTCCA
    CCCAGTGACG CTGCCCCAGC AGCACAGGTA GACGGGGCTG ACCTGGCCTC TCCCATGTCT
    CCTCGAACTA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GGTCCTCTGG CTCAGCCAAT
    AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516170.1
    CDS809..7057
    Translation

    Target ORF information:

    RefSeq Version XM_006516107.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516107.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTCTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCCA CCCAGAAGGT GCTGGACACC 
    AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT
    ATTGACCTTA AACAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA
    AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGTC ACAAGTCTCA AAGAGAGGAA
    TTAGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT
    CAGCGATGGC AATTCCATAG TATTGGGTTG ATTTTAAAGA AACTACTGCA CACAGGAAAC
    TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCTCTTCAG
    GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTCTCAGCA
    CCACAATCCG AATATGGACC TCGGACTTTA GATAATCTTA TTAATCCTCC ACTCAGCCTT
    CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACTCCTCTTG TTCCGCCACA GTCAGGAGAT
    AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT
    CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG
    TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATTTG CAAGGAGAAT
    AGTTTGTATC ATCCTGTACT TGATATCCCA CAGATCAGAC CAAAGCCTCA TTATGTGATG
    ATAAAGAAGG ATGCTGAAAC CAATGAAACA ATATATTGTA CAAAAGAGCC TTTCATCCAG
    GCTCGGGTTA TTGTCATTCG TTGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA
    GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT
    GCTAGTTTGG TTTCCAGAGA AGAAAGCAAA AATGATACTG TTGATAAAGT AGACAAGTCT
    GCAGAGCCTG AGCAGTCTCA TTCCAACACA AGCACTCTCA CGGAGCGAGA GCCTAGCTCT
    TCCAGTCTCT GCAGCATCGA CGAAGAACAT CTCACAGACA TCGAAATTGT TCGCAGAGTG
    TTTTCATCTA AGAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGTCA GGCATTTTTA
    TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTGA AAGTGTATCA AGAATGGATT
    CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAGG ACACTGCGAT CACTTGTTCA
    GATATACCCT GCAGCGAAAC TGTTGCAGAC CATGACAGTG CAATAGAAGA TGGAGAGAAA
    AGAGAAGAGG AAAATGGGAC CAGTACTTCT GAACATGTTC GCAATTCTAG CTGGACAAAA
    AATGGTTCCT ACCAAGAAGC TTTTCATGTC TGTGAAGAAG CCACAGAACA GAATATTCAA
    GCTGGCACTC AGGCCGTTTT GCAGGTGTTT ATTATAAACT CATCGAACAT ATTTCTTCTT
    GAACCTGCAA ATGAAATAAA GAATCTTCTG GATGAGCATA CAGATATGTG TAAACGCATT
    CTTAATATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG
    ATGCTGCTCG TGCTGCTCAG AGTCACGGAG TCCGTACTGA AGATGTCGTC ACAAGCTTTT
    CTGCAGTTCC AGGGGAAAAA AAGCATGACC CTGGCGGGTC GACTTGCAGG ACCACTTTTT
    CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TATACATTTC TCGAGAACTT
    TGGGATGACT TACTTTCGGT ATTGTCATCA TTGACCTATT GGGAAGAATT GGCCACTGAG
    TGGTCACTGA CCATGGAGAC ACTGACTAAA GTTTTGGCTA GAAATTTATA TAGTTTGGAT
    CTCAGTGATT TGCCATTGGA TAAGCTCAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA
    GGAGTTGGAC ACGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT
    CGCGATCAGC CTGGCCAGGC ACCTATGAGA CAGAGAAGTG CAACGACCAC TGGCTCCCCA
    GGAACTGAGA AGGCCAGGAG TATAGTACGG CAAAAAACTG TTGATATTGA TGATGCTCAA
    ATCCTTCCCC GCTCAACCAG AGTCAGACAT TTTTCACAGA GTGAAGACAC TGGAAATGAA
    GTTTTTGGTG CTTTGCATGA GGAACAGCCA CTGCCTCGAA GTAGCAGCAC TTCTGACATC
    TTGGAACCAT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAG CCCCAAACTG
    CCTCCTCTTA ATAGTGAGAC TGGTGGCAAC AGCGCCAATG TTCCTGACCT GATGGATGAG
    TTTATCGCAG AGCGACTTCG AAGTGGCAAT GCCTCGACTA TGACAAGAAG GGGGAGTAGT
    CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG
    CGGCCTGTTG ATGACCCTGG TGTACCCTCC GAATGGACGT CTCCAGCCAG TGCAGGGAGC
    AGTGATCTCA TGAGCTCAGA TAGTCACTCG GATTCCTTCA GTGCCTTCCA GTGTGAGGGA
    CGAAAATTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCATCTTC TGCTGATGTG
    GATTTAGGTT CTGGCCATCA TCAAAGCACT GAGGAGCAGG AAGTGGCCAG CCTAACTACG
    CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC AGAAGTTGCA
    ACCGTTACTG GTTCAGAAAG TGCTTCCCCC GTTCATTCAG CTCTGGGATC CAGGTCGCAG
    ACCCCCTCCC CATCTACACT GAGCAGAGCT CACATAGAAC AGAAGGACCT ACAGCTCGAT
    GAGAAGCTCC ACCACTCTGT TCTTCAGACG CCGGATGACC TAGAAATCAG TGAATTTCCA
    TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT
    ACTGTTATGT GGCGCAGAAT GCTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT
    GAAATACATG CTCAAGTTTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC
    AGAGATAACC TTGGCATTTC AGCTGACAAC CTGACTTCCC CTTCTCCACC CGTCCTGATT
    CCTCCACTAA GAATTCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT
    AAACAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT
    GTATCTCCAA ATAGAGATTT TTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT
    CATATTGACC AGGACATTGT CAATACGATC ATCAAGCACT GCTCACCTCA GTTTTTTTCA
    CTCGGTTTGC CTGGGGCCAC GATGCTTATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG
    GCTTCTTCGG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTA
    GTTTGCTTTC CCAACTTATA TTGTGAATTG CCTGCTCTCC ATCCCAACAT TCCTGATATT
    GCTGTGTCTC AATTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCGGCA
    AGAGATGAGC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGTTTAGG TATTTGGATT
    TGTGAAGAAT TAGTCCACGA ATCTCATCAT CCTCAAATTA AAGAAGCACT GAATGTAATT
    TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCACG TCGCCTGTAA CATGCTTCAC
    ATGCTGGTTC ACTATGTCCC TAGACTTCAG ATTCACCAGC CGCAGTCTCC CCTGAAAATT
    ATTCAGATCC TAATAGCAAC TATCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT
    GAAATGGACA AGAGGTTGGT TGTCTCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC
    TTACCTTTAA AGACACTGCT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAGACC
    GAAAAGTCTG TTCTCAACTG CATTTATAAG GTATTACATG GGTGTGTCTA TGGAGCGCAA
    AGTTTTAGCA ATCCCAAGTA CTTTCCAATA AGCCTTTCTG ATTTGGCATC TGTAGATTAT
    GATCCGTTTA TGCATTTGGA AAGTCTGAAA GAACCTGAAC CTTTACACTC TCCAGATTCA
    GAGCGGTCTT CTAAACTGCA GCCTGTGACA GAAGTGAAAA CTCAGATGCA ACAAGGATTA
    ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA
    ATGAGTGGTG GTCCTGCTAT GCTAACAAGT CAGGTGTGTG AAAATCATGA CAATCATTAC
    AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAACATCCA GTTCTTTGTG
    CTGAATAATA CAACCTTAGT GTCCTGTATC CAGATAAGAT CGGAAGAGAG TATGCCTGGA
    GGAGGTCTAG CTGCTGGCCT TGTGTCAGCC AACTCAAATG TTAGAATCAT AGTACGTGAT
    CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GACCACCCAT TGTAAGTGGT
    TTGCCTGAAC CTACATCTTT CATACTTTCA ATGTCTGACC AAGAGAAACC GGAAGAGCCT
    CCTACATCTA ACGAATGCTT AGAAGACATC GCAGTAAAAG ATGGGCTTTC TCTCCAGCTT
    AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACGATAC GAGAGGAAGA AGATGTTCTT
    GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAGT GTTTACAGAG AACGGGAATC
    TCCCTCAATG TTCCTGCTCC ACAGCCTCTG TGCATTTCTG AAAAACAGGA GAATGATGTC
    ATTAATGCTA TCCTTAAGCA ATACACGGAA GAAAAAGAGT TTGTAGAGAA GCACTTTAAT
    GACTTAAACA TGAAAGCCTC AGAACAAGAT GAGCCAACAC CTCAGAAACC TCAGTCAGCT
    TTTTATTACT GCAGATTGCT TCTTAGTATA TTGGGAATGA ATTCCTGGGA CAAACGGAGG
    AGCTTTCATC TCTTGAAGAA AAATGAAAAG CTGCTCAGAG AACTTAGGAA CTTGGATTCA
    AGGCAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCTGAAGG ACAAGAAGAT
    AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGC
    CTTGGTTGGG AGGTAAACCT TACAAACCAC TGTGGCTTCA TGGGAGGCCT TCAGAAGAAC
    AGAAGCACTG GACTGACCAC TCCCTATTTT GCTACCTCGA CAGTAGAAGT AATATTTCAT
    GTATCTACAC GAATGCCTTC TGATTCTGAT GACTCTCTGA CCAAAAAATT GAGACATCTA
    GGAAATGATG AAGTGCACAT TGTTTGGTCA GAACATACTA GAGACTACAG GAGAGGAATT
    ATTCCTACTG AGTTTGGTGA TGTCCTCATT GTAATATACC CAATGAAAAA CCACATGTTC
    AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT
    ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT
    GCTCTGAAAT CACTGATTCC ATTGTATCAA AATTTCTATG AGGAGAGAGC ACGGTACCTG
    CAGACAATTG TCCAGCACCA TTTAGAGCCA ACAACATTTG AAGACTTTGC AGCACAGGTT
    TTCTCTCCAG CTCCCTACCA TCATTTCCCT GCTGACGCAG GATCCTACCC AGAGATTCCA
    CCCAGTGACG CTGCCCCAGC AGCACAGGTA GACGGGGCTG ACCTGGCCTC TCCCATGTCT
    CCTCGAACTA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GGTCCTCTGG CTCAGCCAAT
    AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.