SPTAN1 cDNA ORF clone, Bos taurus(cattle)

The following SPTAN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTAN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220568 XM_024999031.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220569 XM_024999034.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220570 XM_024999037.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220571 XM_024999038.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220572 XM_024999039.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220573 XM_024999041.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220574 XM_024999043.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220575 XM_024999044.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220576 XM_024999045.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220577 XM_024999046.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa54548 XM_024999028.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa54549 XM_024999030.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa54550 XM_024999032.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa54552 XM_024999036.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa92000 XM_015473653.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa92001 XM_010810456.2
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa92002 XM_010810457.2
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa92003 XM_010810458.2
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa92004 XM_010810460.2
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa37323 NM_001114156.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa24457 XM_024999029.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa24457 XM_005213345.3
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa37323 XM_015473654.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa37323 XM_024999035.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa54551 XM_010810459.2
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa54551 XM_024999033.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa54553 XM_024999040.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa54553 XM_010810461.2
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa54554 XM_024999042.1
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa54554 XM_010810462.2
Latest version!
Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OBa24457 XM_005213345.2 Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220568
    Clone ID Related Accession (Same CDS sequence) XM_024999031.1
    Accession Version XM_024999031.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGCATAGCGT ATCGTCGGGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTGGA AGAATCCGGG
    ACCCTCGAAT CACAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCTATGAGA
    AGTCAGCTCA AGAAGATTGA AGACCTGGGG GCGGCCATGG AGGAAGCGCT GATCCTGGAC
    AACAAGTACA CGGAGCACAG CACCGTGGGC CTGGCCCAGC AGTGGGACCA GCTCGACCAG
    TTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACGACCGGA
    GTGACCGAGG AGGCCCTCAA AGAATTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG
    TCTGGCCGGC TGAACCACCA GGAGTTCAAA TCCTGTCTGC GCTCCCTGGG CTATGACCTG
    CCCATGGTGG AGGAAGGGGA GCCCGACCCT GAGTTCGAGG CCATCCTGGA CACTGTGGAT
    CCTAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CCTTTATGAT CAGCCGTGAG
    ACCGAGAACG TCAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA
    GGGAAGCCTT ATGTGACCAA GGAGGAGCTG TACCAGAACC TGACTCGGGA ACAAGCCGAC
    TACTGCGTCT CCCACATGAA GCCCTACGTG GACGGCAAGG GCCGCGAACT CCCCACTGCT
    TTTGACTACG TCGAGTTCAC CCGCTCGCTC TTCGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854799.1
    CDS4..7425
    Translation

    Target ORF information:

    RefSeq Version XM_024999031.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGCATAGCGT ATCGTCGGGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTGGA AGAATCCGGG
    ACCCTCGAAT CACAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCTATGAGA
    AGTCAGCTCA AGAAGATTGA AGACCTGGGG GCGGCCATGG AGGAAGCGCT GATCCTGGAC
    AACAAGTACA CGGAGCACAG CACCGTGGGC CTGGCCCAGC AGTGGGACCA GCTCGACCAG
    TTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACGACCGGA
    GTGACCGAGG AGGCCCTCAA AGAATTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG
    TCTGGCCGGC TGAACCACCA GGAGTTCAAA TCCTGTCTGC GCTCCCTGGG CTATGACCTG
    CCCATGGTGG AGGAAGGGGA GCCCGACCCT GAGTTCGAGG CCATCCTGGA CACTGTGGAT
    CCTAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CCTTTATGAT CAGCCGTGAG
    ACCGAGAACG TCAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA
    GGGAAGCCTT ATGTGACCAA GGAGGAGCTG TACCAGAACC TGACTCGGGA ACAAGCCGAC
    TACTGCGTCT CCCACATGAA GCCCTACGTG GACGGCAAGG GCCGCGAACT CCCCACTGCT
    TTTGACTACG TCGAGTTCAC CCGCTCGCTC TTCGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220569
    Clone ID Related Accession (Same CDS sequence) XM_024999034.1
    Accession Version XM_024999034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG
    CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC
    ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC
    CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC
    CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC
    TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT
    TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG
    GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC
    ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC
    ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG
    GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC
    TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA
    GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT
    GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC
    TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA
    TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC
    CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA
    CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC
    AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG
    AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG
    CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG
    ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC
    CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG
    GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC
    AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC
    TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT
    CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG
    GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG
    GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA
    GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC
    ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG
    GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC
    CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGG
    TCCTGCATGG TGGAAGAATC CGGGACCCTC GAATCACAGC TTGAAGCTAC CAAACGCAAA
    CACCAGGAGA TTCGAGCTAT GAGAAGTCAG CTCAAGAAGA TTGAAGACCT GGGGGCGGCC
    ATGGAGGAAG CGCTGATCCT GGACAACAAG TACACGGAGC ACAGCACCGT GGGCCTGGCC
    CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC ATGCGCATGC AGCACAACCT GGAGCAGCAG
    ATCCAGGCCA GGAACACGAC CGGAGTGACC GAGGAGGCCC TCAAAGAATT CAGCATGATG
    TTCAAACACT TCGACAAGGA CAAGTCTGGC CGGCTGAACC ACCAGGAGTT CAAATCCTGT
    CTGCGCTCCC TGGGCTATGA CCTGCCCATG GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC
    GAGGCCATCC TGGACACTGT GGATCCTAAC AGGGACGGCC ACGTCTCCCT GCAAGAGTAC
    ATGGCCTTTA TGATCAGCCG TGAGACCGAG AACGTCAAGT CCAGCGAGGA GATTGAGAGC
    GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG CCTTATGTGA CCAAGGAGGA GCTGTACCAG
    AACCTGACTC GGGAACAAGC CGACTACTGC GTCTCCCACA TGAAGCCCTA CGTGGACGGC
    AAGGGCCGCG AACTCCCCAC TGCTTTTGAC TACGTCGAGT TCACCCGCTC GCTCTTCGTG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854802.1
    CDS4..7389
    Translation

    Target ORF information:

    RefSeq Version XM_024999034.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG
    CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC
    ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC
    CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC
    CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC
    TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT
    TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG
    GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC
    ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC
    ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG
    GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC
    TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA
    GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT
    GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC
    TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA
    TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC
    CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA
    CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC
    AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG
    AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG
    CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG
    ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC
    CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG
    GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC
    AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC
    TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT
    CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG
    GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG
    GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA
    GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC
    ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG
    GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC
    CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGG
    TCCTGCATGG TGGAAGAATC CGGGACCCTC GAATCACAGC TTGAAGCTAC CAAACGCAAA
    CACCAGGAGA TTCGAGCTAT GAGAAGTCAG CTCAAGAAGA TTGAAGACCT GGGGGCGGCC
    ATGGAGGAAG CGCTGATCCT GGACAACAAG TACACGGAGC ACAGCACCGT GGGCCTGGCC
    CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC ATGCGCATGC AGCACAACCT GGAGCAGCAG
    ATCCAGGCCA GGAACACGAC CGGAGTGACC GAGGAGGCCC TCAAAGAATT CAGCATGATG
    TTCAAACACT TCGACAAGGA CAAGTCTGGC CGGCTGAACC ACCAGGAGTT CAAATCCTGT
    CTGCGCTCCC TGGGCTATGA CCTGCCCATG GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC
    GAGGCCATCC TGGACACTGT GGATCCTAAC AGGGACGGCC ACGTCTCCCT GCAAGAGTAC
    ATGGCCTTTA TGATCAGCCG TGAGACCGAG AACGTCAAGT CCAGCGAGGA GATTGAGAGC
    GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG CCTTATGTGA CCAAGGAGGA GCTGTACCAG
    AACCTGACTC GGGAACAAGC CGACTACTGC GTCTCCCACA TGAAGCCCTA CGTGGACGGC
    AAGGGCCGCG AACTCCCCAC TGCTTTTGAC TACGTCGAGT TCACCCGCTC GCTCTTCGTG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220570
    Clone ID Related Accession (Same CDS sequence) XM_024999037.1
    Accession Version XM_024999037.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGGTCCTG CATGGTGGAA GAATCCGGGA CCCTCGAATC ACAGCTTGAA
    GCTACCAAAC GCAAACACCA GGAGATTCGA GCTATGAGAA GTCAGCTCAA GAAGATTGAA
    GACCTGGGGG CGGCCATGGA GGAAGCGCTG ATCCTGGACA ACAAGTACAC GGAGCACAGC
    ACCGTGGGCC TGGCCCAGCA GTGGGACCAG CTCGACCAGT TGGGCATGCG CATGCAGCAC
    AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACGACCGGAG TGACCGAGGA GGCCCTCAAA
    GAATTCAGCA TGATGTTCAA ACACTTCGAC AAGGACAAGT CTGGCCGGCT GAACCACCAG
    GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAG
    CCCGACCCTG AGTTCGAGGC CATCCTGGAC ACTGTGGATC CTAACAGGGA CGGCCACGTC
    TCCCTGCAAG AGTACATGGC CTTTATGATC AGCCGTGAGA CCGAGAACGT CAAGTCCAGC
    GAGGAGATTG AGAGCGCCTT CCGGGCCCTG AGCTCTGAAG GGAAGCCTTA TGTGACCAAG
    GAGGAGCTGT ACCAGAACCT GACTCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG
    CCCTACGTGG ACGGCAAGGG CCGCGAACTC CCCACTGCTT TTGACTACGT CGAGTTCACC
    CGCTCGCTCT TCGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854805.1
    CDS120..7520
    Translation

    Target ORF information:

    RefSeq Version XM_024999037.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999037.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGGTCCTG CATGGTGGAA GAATCCGGGA CCCTCGAATC ACAGCTTGAA
    GCTACCAAAC GCAAACACCA GGAGATTCGA GCTATGAGAA GTCAGCTCAA GAAGATTGAA
    GACCTGGGGG CGGCCATGGA GGAAGCGCTG ATCCTGGACA ACAAGTACAC GGAGCACAGC
    ACCGTGGGCC TGGCCCAGCA GTGGGACCAG CTCGACCAGT TGGGCATGCG CATGCAGCAC
    AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACGACCGGAG TGACCGAGGA GGCCCTCAAA
    GAATTCAGCA TGATGTTCAA ACACTTCGAC AAGGACAAGT CTGGCCGGCT GAACCACCAG
    GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAG
    CCCGACCCTG AGTTCGAGGC CATCCTGGAC ACTGTGGATC CTAACAGGGA CGGCCACGTC
    TCCCTGCAAG AGTACATGGC CTTTATGATC AGCCGTGAGA CCGAGAACGT CAAGTCCAGC
    GAGGAGATTG AGAGCGCCTT CCGGGCCCTG AGCTCTGAAG GGAAGCCTTA TGTGACCAAG
    GAGGAGCTGT ACCAGAACCT GACTCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG
    CCCTACGTGG ACGGCAAGGG CCGCGAACTC CCCACTGCTT TTGACTACGT CGAGTTCACC
    CGCTCGCTCT TCGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220571
    Clone ID Related Accession (Same CDS sequence) XM_024999038.1
    Accession Version XM_024999038.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGG TCCTGCATGG TGGAAGAATC CGGGACCCTC
    GAATCACAGC TTGAAGCTAC CAAACGCAAA CACCAGGAGA TTCGAGCTAT GAGAAGTCAG
    CTCAAGAAGA TTGAAGACCT GGGGGCGGCC ATGGAGGAAG CGCTGATCCT GGACAACAAG
    TACACGGAGC ACAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC
    ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACGAC CGGAGTGACC
    GAGGAGGCCC TCAAAGAATT CAGCATGATG TTCAAACACT TCGACAAGGA CAAGTCTGGC
    CGGCTGAACC ACCAGGAGTT CAAATCCTGT CTGCGCTCCC TGGGCTATGA CCTGCCCATG
    GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC GAGGCCATCC TGGACACTGT GGATCCTAAC
    AGGGACGGCC ACGTCTCCCT GCAAGAGTAC ATGGCCTTTA TGATCAGCCG TGAGACCGAG
    AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG
    CCTTATGTGA CCAAGGAGGA GCTGTACCAG AACCTGACTC GGGAACAAGC CGACTACTGC
    GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AACTCCCCAC TGCTTTTGAC
    TACGTCGAGT TCACCCGCTC GCTCTTCGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854806.1
    CDS4..7359
    Translation

    Target ORF information:

    RefSeq Version XM_024999038.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGG TCCTGCATGG TGGAAGAATC CGGGACCCTC
    GAATCACAGC TTGAAGCTAC CAAACGCAAA CACCAGGAGA TTCGAGCTAT GAGAAGTCAG
    CTCAAGAAGA TTGAAGACCT GGGGGCGGCC ATGGAGGAAG CGCTGATCCT GGACAACAAG
    TACACGGAGC ACAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC
    ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACGAC CGGAGTGACC
    GAGGAGGCCC TCAAAGAATT CAGCATGATG TTCAAACACT TCGACAAGGA CAAGTCTGGC
    CGGCTGAACC ACCAGGAGTT CAAATCCTGT CTGCGCTCCC TGGGCTATGA CCTGCCCATG
    GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC GAGGCCATCC TGGACACTGT GGATCCTAAC
    AGGGACGGCC ACGTCTCCCT GCAAGAGTAC ATGGCCTTTA TGATCAGCCG TGAGACCGAG
    AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG
    CCTTATGTGA CCAAGGAGGA GCTGTACCAG AACCTGACTC GGGAACAAGC CGACTACTGC
    GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AACTCCCCAC TGCTTTTGAC
    TACGTCGAGT TCACCCGCTC GCTCTTCGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220572
    Clone ID Related Accession (Same CDS sequence) XM_024999039.1
    Accession Version XM_024999039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG
    GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC
    ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA
    GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA
    GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG
    GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG
    CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC
    CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG
    GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG
    CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG
    GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG
    ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC
    GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC
    GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG
    CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA
    GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA
    GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG
    ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG
    GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG
    AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG
    GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG
    AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC
    AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC
    ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC
    CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG
    AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT
    GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC
    CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC
    CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG
    ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG
    GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG
    CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC
    TTCCACCAGT GGATCCAGGA GACCAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA
    TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC
    AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG
    GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC
    GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT
    TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC
    GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854807.1
    CDS4..7356
    Translation

    Target ORF information:

    RefSeq Version XM_024999039.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG
    GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC
    ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA
    GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA
    GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG
    GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG
    CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC
    CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG
    GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG
    CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG
    GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG
    ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC
    GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC
    GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG
    CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA
    GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA
    GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG
    ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG
    GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG
    AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG
    GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG
    AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC
    AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC
    ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC
    CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG
    AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT
    GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC
    CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC
    CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG
    ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG
    GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG
    CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC
    TTCCACCAGT GGATCCAGGA GACCAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA
    TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC
    AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG
    GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC
    GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT
    TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC
    GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220573
    Clone ID Related Accession (Same CDS sequence) XM_024999041.1
    Accession Version XM_024999041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG
    GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC
    ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA
    GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA
    GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG
    GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG
    CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC
    CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG
    GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG
    CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG
    GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG
    ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC
    GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC
    GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG
    CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA
    GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA
    GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG
    ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG
    GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG
    AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG
    GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG
    AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC
    AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC
    ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC
    CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG
    AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT
    GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC
    CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC
    CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG
    ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG
    GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG
    CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC
    TTCCACCAGT GGATCCAGGA GACCAGAACG TACCTCCTCG ATGGGTCCTG CATGGTGGAA
    GAATCCGGGA CCCTCGAATC ACAGCTTGAA GCTACCAAAC GCAAACACCA GGAGATTCGA
    GCTATGAGAA GTCAGCTCAA GAAGATTGAA GACCTGGGGG CGGCCATGGA GGAAGCGCTG
    ATCCTGGACA ACAAGTACAC GGAGCACAGC ACCGTGGGCC TGGCCCAGCA GTGGGACCAG
    CTCGACCAGT TGGGCATGCG CATGCAGCAC AACCTGGAGC AGCAGATCCA GGCCAGGAAC
    ACGACCGGAG TGACCGAGGA GGCCCTCAAA GAATTCAGCA TGATGTTCAA ACACTTCGAC
    AAGGACAAGT CTGGCCGGCT GAACCACCAG GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC
    TATGACCTGC CCATGGTGGA GGAAGGGGAG CCCGACCCTG AGTTCGAGGC CATCCTGGAC
    ACTGTGGATC CTAACAGGGA CGGCCACGTC TCCCTGCAAG AGTACATGGC CTTTATGATC
    AGCCGTGAGA CCGAGAACGT CAAGTCCAGC GAGGAGATTG AGAGCGCCTT CCGGGCCCTG
    AGCTCTGAAG GGAAGCCTTA TGTGACCAAG GAGGAGCTGT ACCAGAACCT GACTCGGGAA
    CAAGCCGACT ACTGCGTCTC CCACATGAAG CCCTACGTGG ACGGCAAGGG CCGCGAACTC
    CCCACTGCTT TTGACTACGT CGAGTTCACC CGCTCGCTCT TCGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854809.1
    CDS120..7490
    Translation

    Target ORF information:

    RefSeq Version XM_024999041.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG
    GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC
    ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA
    GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA
    GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG
    GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG
    CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC
    CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG
    GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG
    CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG
    GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG
    ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC
    GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC
    GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG
    CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA
    GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA
    GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG
    ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG
    GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG
    AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG
    GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG
    AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC
    AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC
    ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC
    CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG
    AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT
    GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC
    CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC
    CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG
    ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG
    GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG
    CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC
    TTCCACCAGT GGATCCAGGA GACCAGAACG TACCTCCTCG ATGGGTCCTG CATGGTGGAA
    GAATCCGGGA CCCTCGAATC ACAGCTTGAA GCTACCAAAC GCAAACACCA GGAGATTCGA
    GCTATGAGAA GTCAGCTCAA GAAGATTGAA GACCTGGGGG CGGCCATGGA GGAAGCGCTG
    ATCCTGGACA ACAAGTACAC GGAGCACAGC ACCGTGGGCC TGGCCCAGCA GTGGGACCAG
    CTCGACCAGT TGGGCATGCG CATGCAGCAC AACCTGGAGC AGCAGATCCA GGCCAGGAAC
    ACGACCGGAG TGACCGAGGA GGCCCTCAAA GAATTCAGCA TGATGTTCAA ACACTTCGAC
    AAGGACAAGT CTGGCCGGCT GAACCACCAG GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC
    TATGACCTGC CCATGGTGGA GGAAGGGGAG CCCGACCCTG AGTTCGAGGC CATCCTGGAC
    ACTGTGGATC CTAACAGGGA CGGCCACGTC TCCCTGCAAG AGTACATGGC CTTTATGATC
    AGCCGTGAGA CCGAGAACGT CAAGTCCAGC GAGGAGATTG AGAGCGCCTT CCGGGCCCTG
    AGCTCTGAAG GGAAGCCTTA TGTGACCAAG GAGGAGCTGT ACCAGAACCT GACTCGGGAA
    CAAGCCGACT ACTGCGTCTC CCACATGAAG CCCTACGTGG ACGGCAAGGG CCGCGAACTC
    CCCACTGCTT TTGACTACGT CGAGTTCACC CGCTCGCTCT TCGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220574
    Clone ID Related Accession (Same CDS sequence) XM_024999043.1
    Accession Version XM_024999043.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGGTCCTG CATGGTGGAA GAATCCGGGA CCCTCGAATC ACAGCTTGAA
    GCTACCAAAC GCAAACACCA GGAGATTCGA GCTATGAGAA GTCAGCTCAA GAAGATTGAA
    GACCTGGGGG CGGCCATGGA GGAAGCGCTG ATCCTGGACA ACAAGTACAC GGAGCACAGC
    ACCGTGGGCC TGGCCCAGCA GTGGGACCAG CTCGACCAGT TGGGCATGCG CATGCAGCAC
    AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACGACCGGAG TGACCGAGGA GGCCCTCAAA
    GAATTCAGCA TGATGTTCAA ACACTTCGAC AAGGACAAGT CTGGCCGGCT GAACCACCAG
    GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAG
    CCCGACCCTG AGTTCGAGGC CATCCTGGAC ACTGTGGATC CTAACAGGGA CGGCCACGTC
    TCCCTGCAAG AGTACATGGC CTTTATGATC AGCCGTGAGA CCGAGAACGT CAAGTCCAGC
    GAGGAGATTG AGAGCGCCTT CCGGGCCCTG AGCTCTGAAG GGAAGCCTTA TGTGACCAAG
    GAGGAGCTGT ACCAGAACCT GACTCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG
    CCCTACGTGG ACGGCAAGGG CCGCGAACTC CCCACTGCTT TTGACTACGT CGAGTTCACC
    CGCTCGCTCT TCGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854811.1
    CDS120..7460
    Translation

    Target ORF information:

    RefSeq Version XM_024999043.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGGTCCTG CATGGTGGAA GAATCCGGGA CCCTCGAATC ACAGCTTGAA
    GCTACCAAAC GCAAACACCA GGAGATTCGA GCTATGAGAA GTCAGCTCAA GAAGATTGAA
    GACCTGGGGG CGGCCATGGA GGAAGCGCTG ATCCTGGACA ACAAGTACAC GGAGCACAGC
    ACCGTGGGCC TGGCCCAGCA GTGGGACCAG CTCGACCAGT TGGGCATGCG CATGCAGCAC
    AACCTGGAGC AGCAGATCCA GGCCAGGAAC ACGACCGGAG TGACCGAGGA GGCCCTCAAA
    GAATTCAGCA TGATGTTCAA ACACTTCGAC AAGGACAAGT CTGGCCGGCT GAACCACCAG
    GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC TATGACCTGC CCATGGTGGA GGAAGGGGAG
    CCCGACCCTG AGTTCGAGGC CATCCTGGAC ACTGTGGATC CTAACAGGGA CGGCCACGTC
    TCCCTGCAAG AGTACATGGC CTTTATGATC AGCCGTGAGA CCGAGAACGT CAAGTCCAGC
    GAGGAGATTG AGAGCGCCTT CCGGGCCCTG AGCTCTGAAG GGAAGCCTTA TGTGACCAAG
    GAGGAGCTGT ACCAGAACCT GACTCGGGAA CAAGCCGACT ACTGCGTCTC CCACATGAAG
    CCCTACGTGG ACGGCAAGGG CCGCGAACTC CCCACTGCTT TTGACTACGT CGAGTTCACC
    CGCTCGCTCT TCGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220575
    Clone ID Related Accession (Same CDS sequence) XM_024999044.1
    Accession Version XM_024999044.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG
    CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC
    ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC
    CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC
    CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC
    TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT
    TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG
    GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC
    ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC
    ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG
    GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC
    TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA
    GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT
    GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC
    TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA
    TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC
    CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA
    CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC
    AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG
    AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG
    CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG
    ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC
    CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG
    GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC
    AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC
    TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT
    CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG
    GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG
    GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA
    GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC
    ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG
    GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC
    CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGG
    TCCTGCATGG TGGAAGAATC CGGGACCCTC GAATCACAGC TTGAAGCTAC CAAACGCAAA
    CACCAGGAGA TTCGAGCTAT GAGAAGTCAG CTCAAGAAGA TTGAAGACCT GGGGGCGGCC
    ATGGAGGAAG CGCTGATCCT GGACAACAAG TACACGGAGC ACAGCACCGT GGGCCTGGCC
    CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC ATGCGCATGC AGCACAACCT GGAGCAGCAG
    ATCCAGGCCA GGAACACGAC CGGAGTGACC GAGGAGGCCC TCAAAGAATT CAGCATGATG
    TTCAAACACT TCGACAAGGA CAAGTCTGGC CGGCTGAACC ACCAGGAGTT CAAATCCTGT
    CTGCGCTCCC TGGGCTATGA CCTGCCCATG GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC
    GAGGCCATCC TGGACACTGT GGATCCTAAC AGGGACGGCC ACGTCTCCCT GCAAGAGTAC
    ATGGCCTTTA TGATCAGCCG TGAGACCGAG AACGTCAAGT CCAGCGAGGA GATTGAGAGC
    GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG CCTTATGTGA CCAAGGAGGA GCTGTACCAG
    AACCTGACTC GGGAACAAGC CGACTACTGC GTCTCCCACA TGAAGCCCTA CGTGGACGGC
    AAGGGCCGCG AACTCCCCAC TGCTTTTGAC TACGTCGAGT TCACCCGCTC GCTCTTCGTG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854812.1
    CDS120..7445
    Translation

    Target ORF information:

    RefSeq Version XM_024999044.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG
    CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC
    ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC
    CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC
    CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC
    TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT
    TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG
    GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC
    ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC
    ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG
    GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC
    TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA
    GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT
    GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC
    TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA
    TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC
    CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA
    CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC
    AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG
    AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG
    CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG
    ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC
    CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG
    GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC
    AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC
    TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT
    CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG
    GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG
    GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA
    GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC
    ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG
    GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC
    CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGG
    TCCTGCATGG TGGAAGAATC CGGGACCCTC GAATCACAGC TTGAAGCTAC CAAACGCAAA
    CACCAGGAGA TTCGAGCTAT GAGAAGTCAG CTCAAGAAGA TTGAAGACCT GGGGGCGGCC
    ATGGAGGAAG CGCTGATCCT GGACAACAAG TACACGGAGC ACAGCACCGT GGGCCTGGCC
    CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC ATGCGCATGC AGCACAACCT GGAGCAGCAG
    ATCCAGGCCA GGAACACGAC CGGAGTGACC GAGGAGGCCC TCAAAGAATT CAGCATGATG
    TTCAAACACT TCGACAAGGA CAAGTCTGGC CGGCTGAACC ACCAGGAGTT CAAATCCTGT
    CTGCGCTCCC TGGGCTATGA CCTGCCCATG GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC
    GAGGCCATCC TGGACACTGT GGATCCTAAC AGGGACGGCC ACGTCTCCCT GCAAGAGTAC
    ATGGCCTTTA TGATCAGCCG TGAGACCGAG AACGTCAAGT CCAGCGAGGA GATTGAGAGC
    GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG CCTTATGTGA CCAAGGAGGA GCTGTACCAG
    AACCTGACTC GGGAACAAGC CGACTACTGC GTCTCCCACA TGAAGCCCTA CGTGGACGGC
    AAGGGCCGCG AACTCCCCAC TGCTTTTGAC TACGTCGAGT TCACCCGCTC GCTCTTCGTG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220576
    Clone ID Related Accession (Same CDS sequence) XM_024999045.1
    Accession Version XM_024999045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG
    GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC
    ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA
    GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA
    GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG
    GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG
    CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC
    CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG
    GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG
    CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG
    GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG
    ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC
    GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC
    GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG
    CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA
    GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA
    GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG
    ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG
    GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG
    AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG
    GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG
    AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC
    AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC
    ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC
    CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG
    AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT
    GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC
    CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC
    CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG
    ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG
    GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG
    CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC
    TTCCACCAGT GGATCCAGGA GACCAGAACG TACCTCCTCG ATGGGTCCTG CATGGTGGAA
    GAATCCGGGA CCCTCGAATC ACAGCTTGAA GCTACCAAAC GCAAACACCA GGAGATTCGA
    GCTATGAGAA GTCAGCTCAA GAAGATTGAA GACCTGGGGG CGGCCATGGA GGAAGCGCTG
    ATCCTGGACA ACAAGTACAC GGAGCACAGC ACCGTGGGCC TGGCCCAGCA GTGGGACCAG
    CTCGACCAGT TGGGCATGCG CATGCAGCAC AACCTGGAGC AGCAGATCCA GGCCAGGAAC
    ACGACCGGAG TGACCGAGGA GGCCCTCAAA GAATTCAGCA TGATGTTCAA ACACTTCGAC
    AAGGACAAGT CTGGCCGGCT GAACCACCAG GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC
    TATGACCTGC CCATGGTGGA GGAAGGGGAG CCCGACCCTG AGTTCGAGGC CATCCTGGAC
    ACTGTGGATC CTAACAGGGA CGGCCACGTC TCCCTGCAAG AGTACATGGC CTTTATGATC
    AGCCGTGAGA CCGAGAACGT CAAGTCCAGC GAGGAGATTG AGAGCGCCTT CCGGGCCCTG
    AGCTCTGAAG GGAAGCCTTA TGTGACCAAG GAGGAGCTGT ACCAGAACCT GACTCGGGAA
    CAAGCCGACT ACTGCGTCTC CCACATGAAG CCCTACGTGG ACGGCAAGGG CCGCGAACTC
    CCCACTGCTT TTGACTACGT CGAGTTCACC CGCTCGCTCT TCGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854813.1
    CDS120..7430
    Translation

    Target ORF information:

    RefSeq Version XM_024999045.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG
    GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC
    ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA
    GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA
    GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG
    GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG
    CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC
    CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG
    GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG
    CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG
    GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG
    ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC
    GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC
    GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG
    CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA
    GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA
    GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG
    ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG
    GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG
    AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG
    GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG
    AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC
    AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC
    ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC
    CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG
    AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT
    GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC
    CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC
    CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG
    ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG
    GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG
    CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC
    TTCCACCAGT GGATCCAGGA GACCAGAACG TACCTCCTCG ATGGGTCCTG CATGGTGGAA
    GAATCCGGGA CCCTCGAATC ACAGCTTGAA GCTACCAAAC GCAAACACCA GGAGATTCGA
    GCTATGAGAA GTCAGCTCAA GAAGATTGAA GACCTGGGGG CGGCCATGGA GGAAGCGCTG
    ATCCTGGACA ACAAGTACAC GGAGCACAGC ACCGTGGGCC TGGCCCAGCA GTGGGACCAG
    CTCGACCAGT TGGGCATGCG CATGCAGCAC AACCTGGAGC AGCAGATCCA GGCCAGGAAC
    ACGACCGGAG TGACCGAGGA GGCCCTCAAA GAATTCAGCA TGATGTTCAA ACACTTCGAC
    AAGGACAAGT CTGGCCGGCT GAACCACCAG GAGTTCAAAT CCTGTCTGCG CTCCCTGGGC
    TATGACCTGC CCATGGTGGA GGAAGGGGAG CCCGACCCTG AGTTCGAGGC CATCCTGGAC
    ACTGTGGATC CTAACAGGGA CGGCCACGTC TCCCTGCAAG AGTACATGGC CTTTATGATC
    AGCCGTGAGA CCGAGAACGT CAAGTCCAGC GAGGAGATTG AGAGCGCCTT CCGGGCCCTG
    AGCTCTGAAG GGAAGCCTTA TGTGACCAAG GAGGAGCTGT ACCAGAACCT GACTCGGGAA
    CAAGCCGACT ACTGCGTCTC CCACATGAAG CCCTACGTGG ACGGCAAGGG CCGCGAACTC
    CCCACTGCTT TTGACTACGT CGAGTTCACC CGCTCGCTCT TCGTGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220577
    Clone ID Related Accession (Same CDS sequence) XM_024999046.1
    Accession Version XM_024999046.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7293bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG
    GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC
    ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA
    GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA
    GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG
    GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG
    CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC
    CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG
    GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG
    CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG
    GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG
    ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC
    GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC
    GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG
    CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA
    GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA
    GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG
    ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG
    GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG
    AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG
    GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG
    AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC
    AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC
    ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC
    CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG
    AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT
    GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC
    CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC
    CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG
    ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG
    GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG
    CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC
    TTCCACCAGT GGATCCAGGA GACCAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA
    TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC
    AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG
    GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC
    GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT
    TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC
    GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854814.1
    CDS120..7412
    Translation

    Target ORF information:

    RefSeq Version XM_024999046.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG AGCAAGCATC AGAAGCACCA GGCCTTCGAG
    GCAGAACTGC ACGCCAATGC GGACCGGATC CGTGGGGTCA TCGACATGGG CAACTCCCTC
    ATTGACCGCG GGGCCTGCGC CGGCAGCGAG GATGCCGTCA AGGCCCGGCT GGCCGCCTTA
    GCTGACCAGT GGCAGTTCCT GGTGCAGAAG TCGGCTGAGA AGAGCCAGAA GCTGAAAGAA
    GCCAACAAGC AGCAGAACTT CAACACTGGC ATCAAGGACT TCGACTTCTG GCTCTCTGAG
    GTGGAAGCCC TGCTGGCCTC TGAGGATTAT GGCAAAGACC TGGCTTCTGT GAACAACTTG
    CTAAAAAAAC ATCAGCTGCT GGAGGCAGAT ATCTCTGCCC ACGAGGACCG CCTGAAGGAC
    CTCAACAGCC AGGCGGACAG CCTGATGACC AGCAGCGCCT TCGACACCTC CCAGGTGAAG
    GACAAGCGGG ACGCCATCAA CGGCCGTTTC CAGAAGATCA AGAGCATGGC CGCCTCGAGG
    CGAGCTAGGC TGAATGAGTC CCACCGCCTG CACCAGTTTT TCCGGGACAT GGATGATGAG
    GAGTCCTGGA TCAAGGAAAA GAAGCTGCTG GTGAGCTCAG AGGACTACGG CCGTGACCTG
    ACGGGCGTCC AGAACCTGAG GAAGAAGCAC AAACGGCTGG AAGCAGAGCT GGCTGCCCAC
    GAGCCGGCCA TTCAGGGTGT CCTGGACACT GGAAAGAAGC TGTCTGATGA CAACACCATC
    GGGAAGGAGG AGATTCAGCA GCGCCTGGCG CAGTTCGTGG AGCACTGGAA GGAGCTGAAG
    CAGCTGGCAG CTGCCCGGGG CCAGCGGCTA GAAGAATCTT TGGAATATCA GCAGTTTGTA
    GCCAACGTGG AGGAGGAGGA AGCCTGGATC AATGAGAAGA TGACCCTGGT GGCCAGTGAA
    GATTATGGAG ACACTCTTGC TGCCATCCAG GGCTTGCTGA AGAAACACGA AGCCTTCGAG
    ACGGACTTCA CTGTCCACAA GGACCGAGTG AATGATGTCT GCACCAACGG CCAGGACCTG
    GTTAAGAAGA ACAATCACCA TGAGGAGAAC ATCTCTTCCA AGATGAAGGG CTTGAACGGG
    AAAGTGTCAG ACTTGGAGAA GGCTGCCTCC CAGAGGAAGG CCAAGCTCGA CGAGAACTCG
    GCCTTCCTCC AGTTCAACTG GAAAGCCGAT GTGGTGGAGT CCTGGATCGG TGAAAAGGAG
    AACAGCTTGA AGACAGATGA TTATGGTCGA GATCTGTCTT CTGTCCAAAC TCTCCTCACC
    AAACAGGAAA CCTTCGATGC TGGCCTGCAG GCCTTCCAGC AGGAGGGCAT CGCCAACATC
    ACTGCCCTCA AAGATCAGCT GCTGGCCGCC AAGCACGTTC AGTCCAAGGC CATCGAGGCC
    CGCCATGCTG CCCTCATGAA GAGGTGGAGC CAGCTCCTGG CCAACTCGGC CACCCGGAAG
    AAGAAGCTGC TGGAGGCACA GAGTCACTTC CGCAAGGTGG AGGATCTCTT CCTGACCTTT
    GCTAAAAAGG CTTCAGCCTT CAACAGCTGG TTTGAAAACG CAGAGGAGGA CTTAACCGAC
    CCAGTGCGCT GCAACTCTCT GGAAGAGATC AAGGCCTTGA GAGAGGCCCA CGACGCCTTC
    CGCTCCTCGT TGAGCTCGGC CCAGGCCGAC TTCAACCAGC TGGCAGAGCT GGACCGCCAG
    ATCAAGAGCT TCCGCGTGGC CTCCAACCCC TACACCTGGT TCACCATGGA GGCCCTGGAG
    GAGACCTGGA GAAACCTACA GAAGATCATC AAGGAAAGGG AGCTGGAGCT GCAGAAGGAG
    CAGCGACGGC AGGAGGAGAA TGACAAGCTG CGCCAGGAGT TCGCCCAGCA TGCCAACGCC
    TTCCACCAGT GGATCCAGGA GACCAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA
    TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC
    AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG
    GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC
    GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT
    TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC
    GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54548
    Clone ID Related Accession (Same CDS sequence) XM_024999028.1
    Accession Version XM_024999028.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGCATAGCGT ATCGTCGGGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTGGA AGAATCCGGG
    ACCCTCGAAT CACAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCTATGAGA
    AGTCAGCTCA AGAAGATTGA AGACCTGGGG GCGGCCATGG AGGAAGCGCT GATCCTGGAC
    AACAAGTACA CGGAGCACAG CACCGTGGGC CTGGCCCAGC AGTGGGACCA GCTCGACCAG
    TTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACGACCGGA
    GTGACCGAGG AGGCCCTCAA AGAATTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG
    TCTGGCCGGC TGAACCACCA GGAGTTCAAA TCCTGTCTGC GCTCCCTGGG CTATGACCTG
    CCCATGGTGG AGGAAGGGGA GCCCGACCCT GAGTTCGAGG CCATCCTGGA CACTGTGGAT
    CCTAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CCTTTATGAT CAGCCGTGAG
    ACCGAGAACG TCAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA
    GGGAAGCCTT ATGTGACCAA GGAGGAGCTG TACCAGAACC TGACTCGGGA ACAAGCCGAC
    TACTGCGTCT CCCACATGAA GCCCTACGTG GACGGCAAGG GCCGCGAACT CCCCACTGCT
    TTTGACTACG TCGAGTTCAC CCGCTCGCTC TTCGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854796.1
    CDS4..7485
    Translation

    Target ORF information:

    RefSeq Version XM_024999028.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999028.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGCATAGCGT ATCGTCGGGT CATTCGTGTC
    TATCAGTATG AAGTTGGGGA TGATCTGTCT GGAAGGTCCT GCATGGTGGA AGAATCCGGG
    ACCCTCGAAT CACAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCTATGAGA
    AGTCAGCTCA AGAAGATTGA AGACCTGGGG GCGGCCATGG AGGAAGCGCT GATCCTGGAC
    AACAAGTACA CGGAGCACAG CACCGTGGGC CTGGCCCAGC AGTGGGACCA GCTCGACCAG
    TTGGGCATGC GCATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACGACCGGA
    GTGACCGAGG AGGCCCTCAA AGAATTCAGC ATGATGTTCA AACACTTCGA CAAGGACAAG
    TCTGGCCGGC TGAACCACCA GGAGTTCAAA TCCTGTCTGC GCTCCCTGGG CTATGACCTG
    CCCATGGTGG AGGAAGGGGA GCCCGACCCT GAGTTCGAGG CCATCCTGGA CACTGTGGAT
    CCTAACAGGG ACGGCCACGT CTCCCTGCAA GAGTACATGG CCTTTATGAT CAGCCGTGAG
    ACCGAGAACG TCAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA
    GGGAAGCCTT ATGTGACCAA GGAGGAGCTG TACCAGAACC TGACTCGGGA ACAAGCCGAC
    TACTGCGTCT CCCACATGAA GCCCTACGTG GACGGCAAGG GCCGCGAACT CCCCACTGCT
    TTTGACTACG TCGAGTTCAC CCGCTCGCTC TTCGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54549
    Clone ID Related Accession (Same CDS sequence) XM_024999030.1
    Accession Version XM_024999030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7449bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG
    CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC
    ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC
    CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC
    CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC
    TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT
    TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG
    GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC
    ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC
    ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG
    GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC
    TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA
    GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT
    GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC
    TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA
    TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC
    CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA
    CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC
    AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG
    AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG
    CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG
    ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC
    CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG
    GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC
    AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC
    TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT
    CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG
    GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG
    GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA
    GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC
    ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG
    GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC
    CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGC
    ATAGCGTATC GTCGGGTCAT TCGTGTCTAT CAGTATGAAG TTGGGGATGA TCTGTCTGGA
    AGGTCCTGCA TGGTGGAAGA ATCCGGGACC CTCGAATCAC AGCTTGAAGC TACCAAACGC
    AAACACCAGG AGATTCGAGC TATGAGAAGT CAGCTCAAGA AGATTGAAGA CCTGGGGGCG
    GCCATGGAGG AAGCGCTGAT CCTGGACAAC AAGTACACGG AGCACAGCAC CGTGGGCCTG
    GCCCAGCAGT GGGACCAGCT CGACCAGTTG GGCATGCGCA TGCAGCACAA CCTGGAGCAG
    CAGATCCAGG CCAGGAACAC GACCGGAGTG ACCGAGGAGG CCCTCAAAGA ATTCAGCATG
    ATGTTCAAAC ACTTCGACAA GGACAAGTCT GGCCGGCTGA ACCACCAGGA GTTCAAATCC
    TGTCTGCGCT CCCTGGGCTA TGACCTGCCC ATGGTGGAGG AAGGGGAGCC CGACCCTGAG
    TTCGAGGCCA TCCTGGACAC TGTGGATCCT AACAGGGACG GCCACGTCTC CCTGCAAGAG
    TACATGGCCT TTATGATCAG CCGTGAGACC GAGAACGTCA AGTCCAGCGA GGAGATTGAG
    AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG AAGCCTTATG TGACCAAGGA GGAGCTGTAC
    CAGAACCTGA CTCGGGAACA AGCCGACTAC TGCGTCTCCC ACATGAAGCC CTACGTGGAC
    GGCAAGGGCC GCGAACTCCC CACTGCTTTT GACTACGTCG AGTTCACCCG CTCGCTCTTC
    GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854798.1
    CDS4..7452
    Translation

    Target ORF information:

    RefSeq Version XM_024999030.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGG AGCTGAACGA GCGCTGGCGG
    TCCCTGCAGC AGCTGGCTGA GGAGCGGAGC CAACTCTTGG GCAGTGCCCA TGAAGTGCAG
    CGGTTCCACA GAGATGCTGA CGAAACCAAA GAGTGGATCG AAGAGAAGAA CCAGGCTCTA
    AACACAGACA ACTACGGCCA CGACCTGGCC AGTGTCCAGG CCCTGCAGCG CAAGCACGAG
    GGCTTCGAGA GGGACCTCGC AGCCCTTGGT GACAAGGTGA ACTCGCTCGG GGAGACAGCC
    GAGCGCCTGA TCCAGTCCCA CCCCGAGTCG GCAGAGGACC TGCAGGAGAA GTGCACTGAG
    CTGAACCAGG CATGGAGCAG CCTGGGCAAG CGTGCCGACC AGCGCAAGGC CAAGCTGGGC
    GACTCCCACG ACCTGCAGCG CTTCCTCAGC GACTTCCGGG ACCTCATGTC TTGGATCAAT
    GGAATCCGAG GTCTGGTGTC CTCAGACGAG CTAGCCAAGG ATGTCACCGG AGCTGAGGCC
    CTGCTGGAGC GACACCAGGA ACACCGGACA GAAATTGATG CCAGAGCTGG CACTTTCCAG
    GCATTCGAGC AGTTTGGGCA GCAGCTGTTG GCTCATGGGC ACTACGCCAG CCCCGAGATC
    AAGGAGAAGC TCGATGTTCT AGACCAGGAG CGTGCAGACC TTGAGAAGGC CTGGGTCCAG
    CGCAGGATGA TGCTGGATCA GTGCCTTGAA TTGCAGCTGT TCCATCGGGA TTGCGAGCAG
    GCCGAGAACT GGATGGCTGC CCGAGAGGCC TTTCTGAACA CAGAAGACAA AGGGGACTCG
    CTAGACAGTG TGGAGGCTCT GATCAAAAAA CACGAGGACT TTGATAAAGC AATTAATGTC
    CAGGAGGAGA AGATTGCTGC CCTGCAGTCC TTTGCTGACC AGCTCATCGC TGGTGGCCAC
    TACGCCAAAG GCGACATCTC CAGCCGGCGC AATGAGGTTC TGGACAGGTG GCGCCGTCTA
    AAAGCTCAGA TGATTGAGAA GAGGTCAAAG CTGGGAGAAT CACAGACCCT CCAGCAGTTC
    AGCCGGGATG TAGACGAGAT TGAAGCTTGG ATCAGTGAAA AATTACAGAC TGCAAGTGAT
    GAGTCATATA AGGACCCCAC CAACATCCAG CTTTCCAAGC TGCTGAGCAA GCATCAGAAG
    CACCAGGCCT TCGAGGCAGA ACTGCACGCC AATGCGGACC GGATCCGTGG GGTCATCGAC
    ATGGGCAACT CCCTCATTGA CCGCGGGGCC TGCGCCGGCA GCGAGGATGC CGTCAAGGCC
    CGGCTGGCCG CCTTAGCTGA CCAGTGGCAG TTCCTGGTGC AGAAGTCGGC TGAGAAGAGC
    CAGAAGCTGA AAGAAGCCAA CAAGCAGCAG AACTTCAACA CTGGCATCAA GGACTTCGAC
    TTCTGGCTCT CTGAGGTGGA AGCCCTGCTG GCCTCTGAGG ATTATGGCAA AGACCTGGCT
    TCTGTGAACA ACTTGCTAAA AAAACATCAG CTGCTGGAGG CAGATATCTC TGCCCACGAG
    GACCGCCTGA AGGACCTCAA CAGCCAGGCG GACAGCCTGA TGACCAGCAG CGCCTTCGAC
    ACCTCCCAGG TGAAGGACAA GCGGGACGCC ATCAACGGCC GTTTCCAGAA GATCAAGAGC
    ATGGCCGCCT CGAGGCGAGC TAGGCTGAAT GAGTCCCACC GCCTGCACCA GTTTTTCCGG
    GACATGGATG ATGAGGAGTC CTGGATCAAG GAAAAGAAGC TGCTGGTGAG CTCAGAGGAC
    TACGGCCGTG ACCTGACGGG CGTCCAGAAC CTGAGGAAGA AGCACAAACG GCTGGAAGCA
    GAGCTGGCTG CCCACGAGCC GGCCATTCAG GGTGTCCTGG ACACTGGAAA GAAGCTGTCT
    GATGACAACA CCATCGGGAA GGAGGAGATT CAGCAGCGCC TGGCGCAGTT CGTGGAGCAC
    TGGAAGGAGC TGAAGCAGCT GGCAGCTGCC CGGGGCCAGC GGCTAGAAGA ATCTTTGGAA
    TATCAGCAGT TTGTAGCCAA CGTGGAGGAG GAGGAAGCCT GGATCAATGA GAAGATGACC
    CTGGTGGCCA GTGAAGATTA TGGAGACACT CTTGCTGCCA TCCAGGGCTT GCTGAAGAAA
    CACGAAGCCT TCGAGACGGA CTTCACTGTC CACAAGGACC GAGTGAATGA TGTCTGCACC
    AACGGCCAGG ACCTGGTTAA GAAGAACAAT CACCATGAGG AGAACATCTC TTCCAAGATG
    AAGGGCTTGA ACGGGAAAGT GTCAGACTTG GAGAAGGCTG CCTCCCAGAG GAAGGCCAAG
    CTCGACGAGA ACTCGGCCTT CCTCCAGTTC AACTGGAAAG CCGATGTGGT GGAGTCCTGG
    ATCGGTGAAA AGGAGAACAG CTTGAAGACA GATGATTATG GTCGAGATCT GTCTTCTGTC
    CAAACTCTCC TCACCAAACA GGAAACCTTC GATGCTGGCC TGCAGGCCTT CCAGCAGGAG
    GGCATCGCCA ACATCACTGC CCTCAAAGAT CAGCTGCTGG CCGCCAAGCA CGTTCAGTCC
    AAGGCCATCG AGGCCCGCCA TGCTGCCCTC ATGAAGAGGT GGAGCCAGCT CCTGGCCAAC
    TCGGCCACCC GGAAGAAGAA GCTGCTGGAG GCACAGAGTC ACTTCCGCAA GGTGGAGGAT
    CTCTTCCTGA CCTTTGCTAA AAAGGCTTCA GCCTTCAACA GCTGGTTTGA AAACGCAGAG
    GAGGACTTAA CCGACCCAGT GCGCTGCAAC TCTCTGGAAG AGATCAAGGC CTTGAGAGAG
    GCCCACGACG CCTTCCGCTC CTCGTTGAGC TCGGCCCAGG CCGACTTCAA CCAGCTGGCA
    GAGCTGGACC GCCAGATCAA GAGCTTCCGC GTGGCCTCCA ACCCCTACAC CTGGTTCACC
    ATGGAGGCCC TGGAGGAGAC CTGGAGAAAC CTACAGAAGA TCATCAAGGA AAGGGAGCTG
    GAGCTGCAGA AGGAGCAGCG ACGGCAGGAG GAGAATGACA AGCTGCGCCA GGAGTTCGCC
    CAGCATGCCA ACGCCTTCCA CCAGTGGATC CAGGAGACCA GAACGTACCT CCTCGATGGC
    ATAGCGTATC GTCGGGTCAT TCGTGTCTAT CAGTATGAAG TTGGGGATGA TCTGTCTGGA
    AGGTCCTGCA TGGTGGAAGA ATCCGGGACC CTCGAATCAC AGCTTGAAGC TACCAAACGC
    AAACACCAGG AGATTCGAGC TATGAGAAGT CAGCTCAAGA AGATTGAAGA CCTGGGGGCG
    GCCATGGAGG AAGCGCTGAT CCTGGACAAC AAGTACACGG AGCACAGCAC CGTGGGCCTG
    GCCCAGCAGT GGGACCAGCT CGACCAGTTG GGCATGCGCA TGCAGCACAA CCTGGAGCAG
    CAGATCCAGG CCAGGAACAC GACCGGAGTG ACCGAGGAGG CCCTCAAAGA ATTCAGCATG
    ATGTTCAAAC ACTTCGACAA GGACAAGTCT GGCCGGCTGA ACCACCAGGA GTTCAAATCC
    TGTCTGCGCT CCCTGGGCTA TGACCTGCCC ATGGTGGAGG AAGGGGAGCC CGACCCTGAG
    TTCGAGGCCA TCCTGGACAC TGTGGATCCT AACAGGGACG GCCACGTCTC CCTGCAAGAG
    TACATGGCCT TTATGATCAG CCGTGAGACC GAGAACGTCA AGTCCAGCGA GGAGATTGAG
    AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG AAGCCTTATG TGACCAAGGA GGAGCTGTAC
    CAGAACCTGA CTCGGGAACA AGCCGACTAC TGCGTCTCCC ACATGAAGCC CTACGTGGAC
    GGCAAGGGCC GCGAACTCCC CACTGCTTTT GACTACGTCG AGTTCACCCG CTCGCTCTTC
    GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54550
    Clone ID Related Accession (Same CDS sequence) XM_024999032.1
    Accession Version XM_024999032.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG
    ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA
    GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG
    GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC
    CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC
    CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT
    ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT
    CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC
    GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854800.1
    CDS121..7557
    Translation

    Target ORF information:

    RefSeq Version XM_024999032.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG
    ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA
    GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG
    GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC
    CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC
    CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT
    ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT
    CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC
    GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54552
    Clone ID Related Accession (Same CDS sequence) XM_024999036.1
    Accession Version XM_024999036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGG TCCTGCATGG TGGAAGAATC CGGGACCCTC
    GAATCACAGC TTGAAGCTAC CAAACGCAAA CACCAGGAGA TTCGAGCTAT GAGAAGTCAG
    CTCAAGAAGA TTGAAGACCT GGGGGCGGCC ATGGAGGAAG CGCTGATCCT GGACAACAAG
    TACACGGAGC ACAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC
    ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACGAC CGGAGTGACC
    GAGGAGGCCC TCAAAGAATT CAGCATGATG TTCAAACACT TCGACAAGGA CAAGTCTGGC
    CGGCTGAACC ACCAGGAGTT CAAATCCTGT CTGCGCTCCC TGGGCTATGA CCTGCCCATG
    GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC GAGGCCATCC TGGACACTGT GGATCCTAAC
    AGGGACGGCC ACGTCTCCCT GCAAGAGTAC ATGGCCTTTA TGATCAGCCG TGAGACCGAG
    AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG
    CCTTATGTGA CCAAGGAGGA GCTGTACCAG AACCTGACTC GGGAACAAGC CGACTACTGC
    GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AACTCCCCAC TGCTTTTGAC
    TACGTCGAGT TCACCCGCTC GCTCTTCGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854804.1
    CDS136..7551
    Translation

    Target ORF information:

    RefSeq Version XM_024999036.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGG TCCTGCATGG TGGAAGAATC CGGGACCCTC
    GAATCACAGC TTGAAGCTAC CAAACGCAAA CACCAGGAGA TTCGAGCTAT GAGAAGTCAG
    CTCAAGAAGA TTGAAGACCT GGGGGCGGCC ATGGAGGAAG CGCTGATCCT GGACAACAAG
    TACACGGAGC ACAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCTCGA CCAGTTGGGC
    ATGCGCATGC AGCACAACCT GGAGCAGCAG ATCCAGGCCA GGAACACGAC CGGAGTGACC
    GAGGAGGCCC TCAAAGAATT CAGCATGATG TTCAAACACT TCGACAAGGA CAAGTCTGGC
    CGGCTGAACC ACCAGGAGTT CAAATCCTGT CTGCGCTCCC TGGGCTATGA CCTGCCCATG
    GTGGAGGAAG GGGAGCCCGA CCCTGAGTTC GAGGCCATCC TGGACACTGT GGATCCTAAC
    AGGGACGGCC ACGTCTCCCT GCAAGAGTAC ATGGCCTTTA TGATCAGCCG TGAGACCGAG
    AACGTCAAGT CCAGCGAGGA GATTGAGAGC GCCTTCCGGG CCCTGAGCTC TGAAGGGAAG
    CCTTATGTGA CCAAGGAGGA GCTGTACCAG AACCTGACTC GGGAACAAGC CGACTACTGC
    GTCTCCCACA TGAAGCCCTA CGTGGACGGC AAGGGCCGCG AACTCCCCAC TGCTTTTGAC
    TACGTCGAGT TCACCCGCTC GCTCTTCGTG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92000
    Clone ID Related Accession (Same CDS sequence) XM_015473653.1
    Accession Version XM_015473653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG
    GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC
    TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG
    ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC
    CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG
    GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG
    GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC
    GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC
    AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT
    GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA
    GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG
    CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG
    AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG
    GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT
    GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG
    GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA
    GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT
    GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG
    GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC
    GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT
    GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG
    GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC
    CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC
    GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC
    CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG
    AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG
    AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC
    CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG
    CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC
    CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA
    GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT
    GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG
    GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG
    GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG
    AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT
    TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG
    AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC
    AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG
    GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC
    CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC
    TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG
    AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT
    GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG
    ACCAGAACGT ACCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA
    TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC
    AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG
    GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC
    GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT
    TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC
    GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329139.1
    CDS55..7587
    Translation

    Target ORF information:

    RefSeq Version XM_015473653.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG
    GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC
    TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG
    ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC
    CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG
    GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG
    GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC
    GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC
    AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT
    GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA
    GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG
    CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG
    AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG
    GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT
    GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG
    GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA
    GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT
    GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG
    GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC
    GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT
    GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG
    GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC
    CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC
    GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC
    CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG
    AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG
    AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC
    CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG
    CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC
    CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA
    GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT
    GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG
    GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG
    GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG
    AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT
    TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG
    AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC
    AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG
    GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC
    CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC
    TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG
    AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT
    GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG
    ACCAGAACGT ACCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA
    TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC
    AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG
    GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC
    GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT
    TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC
    GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92001
    Clone ID Related Accession (Same CDS sequence) XM_010810456.2
    Accession Version XM_010810456.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG
    GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC
    TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG
    ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC
    CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG
    GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG
    GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC
    GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC
    AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT
    GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA
    GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG
    CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG
    AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG
    GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT
    GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG
    GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA
    GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT
    GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG
    CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGTGGG
    GTCATCGACA TGGGCAACTC CCTCATTGAC CGCGGGGCCT GCGCCGGCAG CGAGGATGCC
    GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT
    GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGCATCAAG
    GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT
    GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC
    GCCTTCGACA CCTCCCAGGT GAAGGACAAG CGGGACGCCA TCAACGGCCG TTTCCAGAAG
    ATCAAGAGCA TGGCCGCCTC GAGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG
    TTTTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AAAAGAAGCT GCTGGTGAGC
    TCAGAGGACT ACGGCCGTGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG
    CTGGAAGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG
    AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCCC GGGGCCAGCG GCTAGAAGAA
    TCTTTGGAAT ATCAGCAGTT TGTAGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG
    AAGATGACCC TGGTGGCCAG TGAAGATTAT GGAGACACTC TTGCTGCCAT CCAGGGCTTG
    CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG AGTGAATGAT
    GTCTGCACCA ACGGCCAGGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG
    AAGGCCAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAAGC CGATGTGGTG
    GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG
    TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    GTTCAGTCCA AGGCCATCGA GGCCCGCCAT GCTGCCCTCA TGAAGAGGTG GAGCCAGCTC
    CTGGCCAACT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAGGATC TCTTCCTGAC CTTTGCTAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA
    AACGCAGAGG AGGACTTAAC CGACCCAGTG CGCTGCAACT CTCTGGAAGA GATCAAGGCC
    TTGAGAGAGG CCCACGACGC CTTCCGCTCC TCGTTGAGCT CGGCCCAGGC CGACTTCAAC
    CAGCTGGCAG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGAAACC TACAGAAGAT CATCAAGGAA
    AGGGAGCTGG AGCTGCAGAA GGAGCAGCGA CGGCAGGAGG AGAATGACAA GCTGCGCCAG
    GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTACCTC
    CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT
    CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCCGGGACCC TCGAATCACA GCTTGAAGCT
    ACCAAACGCA AACACCAGGA GATTCGAGCT ATGAGAAGTC AGCTCAAGAA GATTGAAGAC
    CTGGGGGCGG CCATGGAGGA AGCGCTGATC CTGGACAACA AGTACACGGA GCACAGCACC
    GTGGGCCTGG CCCAGCAGTG GGACCAGCTC GACCAGTTGG GCATGCGCAT GCAGCACAAC
    CTGGAGCAGC AGATCCAGGC CAGGAACACG ACCGGAGTGA CCGAGGAGGC CCTCAAAGAA
    TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCTG GCCGGCTGAA CCACCAGGAG
    TTCAAATCCT GTCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCC
    GACCCTGAGT TCGAGGCCAT CCTGGACACT GTGGATCCTA ACAGGGACGG CCACGTCTCC
    CTGCAAGAGT ACATGGCCTT TATGATCAGC CGTGAGACCG AGAACGTCAA GTCCAGCGAG
    GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCTGAAGGGA AGCCTTATGT GACCAAGGAG
    GAGCTGTACC AGAACCTGAC TCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC
    TACGTGGACG GCAAGGGCCG CGAACTCCCC ACTGCTTTTG ACTACGTCGA GTTCACCCGC
    TCGCTCTTCG TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808758.2
    CDS55..7572
    Translation

    Target ORF information:

    RefSeq Version XM_010810456.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810456.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG
    GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC
    TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG
    ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC
    CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG
    GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG
    GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC
    GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC
    AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT
    GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA
    GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG
    CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG
    AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG
    GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT
    GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG
    GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA
    GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT
    GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG
    CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGTGGG
    GTCATCGACA TGGGCAACTC CCTCATTGAC CGCGGGGCCT GCGCCGGCAG CGAGGATGCC
    GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT
    GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGCATCAAG
    GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT
    GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC
    GCCTTCGACA CCTCCCAGGT GAAGGACAAG CGGGACGCCA TCAACGGCCG TTTCCAGAAG
    ATCAAGAGCA TGGCCGCCTC GAGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG
    TTTTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AAAAGAAGCT GCTGGTGAGC
    TCAGAGGACT ACGGCCGTGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG
    CTGGAAGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG
    AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCCC GGGGCCAGCG GCTAGAAGAA
    TCTTTGGAAT ATCAGCAGTT TGTAGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG
    AAGATGACCC TGGTGGCCAG TGAAGATTAT GGAGACACTC TTGCTGCCAT CCAGGGCTTG
    CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG AGTGAATGAT
    GTCTGCACCA ACGGCCAGGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG
    AAGGCCAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAAGC CGATGTGGTG
    GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG
    TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    GTTCAGTCCA AGGCCATCGA GGCCCGCCAT GCTGCCCTCA TGAAGAGGTG GAGCCAGCTC
    CTGGCCAACT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAGGATC TCTTCCTGAC CTTTGCTAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA
    AACGCAGAGG AGGACTTAAC CGACCCAGTG CGCTGCAACT CTCTGGAAGA GATCAAGGCC
    TTGAGAGAGG CCCACGACGC CTTCCGCTCC TCGTTGAGCT CGGCCCAGGC CGACTTCAAC
    CAGCTGGCAG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGAAACC TACAGAAGAT CATCAAGGAA
    AGGGAGCTGG AGCTGCAGAA GGAGCAGCGA CGGCAGGAGG AGAATGACAA GCTGCGCCAG
    GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTACCTC
    CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT
    CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCCGGGACCC TCGAATCACA GCTTGAAGCT
    ACCAAACGCA AACACCAGGA GATTCGAGCT ATGAGAAGTC AGCTCAAGAA GATTGAAGAC
    CTGGGGGCGG CCATGGAGGA AGCGCTGATC CTGGACAACA AGTACACGGA GCACAGCACC
    GTGGGCCTGG CCCAGCAGTG GGACCAGCTC GACCAGTTGG GCATGCGCAT GCAGCACAAC
    CTGGAGCAGC AGATCCAGGC CAGGAACACG ACCGGAGTGA CCGAGGAGGC CCTCAAAGAA
    TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCTG GCCGGCTGAA CCACCAGGAG
    TTCAAATCCT GTCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCC
    GACCCTGAGT TCGAGGCCAT CCTGGACACT GTGGATCCTA ACAGGGACGG CCACGTCTCC
    CTGCAAGAGT ACATGGCCTT TATGATCAGC CGTGAGACCG AGAACGTCAA GTCCAGCGAG
    GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCTGAAGGGA AGCCTTATGT GACCAAGGAG
    GAGCTGTACC AGAACCTGAC TCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC
    TACGTGGACG GCAAGGGCCG CGAACTCCCC ACTGCTTTTG ACTACGTCGA GTTCACCCGC
    TCGCTCTTCG TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92002
    Clone ID Related Accession (Same CDS sequence) XM_010810457.2
    Accession Version XM_010810457.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG
    GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGGAGCT GAACGAGCGC TGGCGGTCCC TGCAGCAGCT GGCTGAGGAG
    CGGAGCCAAC TCTTGGGCAG TGCCCATGAA GTGCAGCGGT TCCACAGAGA TGCTGACGAA
    ACCAAAGAGT GGATCGAAGA GAAGAACCAG GCTCTAAACA CAGACAACTA CGGCCACGAC
    CTGGCCAGTG TCCAGGCCCT GCAGCGCAAG CACGAGGGCT TCGAGAGGGA CCTCGCAGCC
    CTTGGTGACA AGGTGAACTC GCTCGGGGAG ACAGCCGAGC GCCTGATCCA GTCCCACCCC
    GAGTCGGCAG AGGACCTGCA GGAGAAGTGC ACTGAGCTGA ACCAGGCATG GAGCAGCCTG
    GGCAAGCGTG CCGACCAGCG CAAGGCCAAG CTGGGCGACT CCCACGACCT GCAGCGCTTC
    CTCAGCGACT TCCGGGACCT CATGTCTTGG ATCAATGGAA TCCGAGGTCT GGTGTCCTCA
    GACGAGCTAG CCAAGGATGT CACCGGAGCT GAGGCCCTGC TGGAGCGACA CCAGGAACAC
    CGGACAGAAA TTGATGCCAG AGCTGGCACT TTCCAGGCAT TCGAGCAGTT TGGGCAGCAG
    CTGTTGGCTC ATGGGCACTA CGCCAGCCCC GAGATCAAGG AGAAGCTCGA TGTTCTAGAC
    CAGGAGCGTG CAGACCTTGA GAAGGCCTGG GTCCAGCGCA GGATGATGCT GGATCAGTGC
    CTTGAATTGC AGCTGTTCCA TCGGGATTGC GAGCAGGCCG AGAACTGGAT GGCTGCCCGA
    GAGGCCTTTC TGAACACAGA AGACAAAGGG GACTCGCTAG ACAGTGTGGA GGCTCTGATC
    AAAAAACACG AGGACTTTGA TAAAGCAATT AATGTCCAGG AGGAGAAGAT TGCTGCCCTG
    CAGTCCTTTG CTGACCAGCT CATCGCTGGT GGCCACTACG CCAAAGGCGA CATCTCCAGC
    CGGCGCAATG AGGTTCTGGA CAGGTGGCGC CGTCTAAAAG CTCAGATGAT TGAGAAGAGG
    TCAAAGCTGG GAGAATCACA GACCCTCCAG CAGTTCAGCC GGGATGTAGA CGAGATTGAA
    GCTTGGATCA GTGAAAAATT ACAGACTGCA AGTGATGAGT CATATAAGGA CCCCACCAAC
    ATCCAGCTTT CCAAGCTGCT GAGCAAGCAT CAGAAGCACC AGGCCTTCGA GGCAGAACTG
    CACGCCAATG CGGACCGGAT CCGTGGGGTC ATCGACATGG GCAACTCCCT CATTGACCGC
    GGGGCCTGCG CCGGCAGCGA GGATGCCGTC AAGGCCCGGC TGGCCGCCTT AGCTGACCAG
    TGGCAGTTCC TGGTGCAGAA GTCGGCTGAG AAGAGCCAGA AGCTGAAAGA AGCCAACAAG
    CAGCAGAACT TCAACACTGG CATCAAGGAC TTCGACTTCT GGCTCTCTGA GGTGGAAGCC
    CTGCTGGCCT CTGAGGATTA TGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTAAAAAAA
    CATCAGCTGC TGGAGGCAGA TATCTCTGCC CACGAGGACC GCCTGAAGGA CCTCAACAGC
    CAGGCGGACA GCCTGATGAC CAGCAGCGCC TTCGACACCT CCCAGGTGAA GGACAAGCGG
    GACGCCATCA ACGGCCGTTT CCAGAAGATC AAGAGCATGG CCGCCTCGAG GCGAGCTAGG
    CTGAATGAGT CCCACCGCCT GCACCAGTTT TTCCGGGACA TGGATGATGA GGAGTCCTGG
    ATCAAGGAAA AGAAGCTGCT GGTGAGCTCA GAGGACTACG GCCGTGACCT GACGGGCGTC
    CAGAACCTGA GGAAGAAGCA CAAACGGCTG GAAGCAGAGC TGGCTGCCCA CGAGCCGGCC
    ATTCAGGGTG TCCTGGACAC TGGAAAGAAG CTGTCTGATG ACAACACCAT CGGGAAGGAG
    GAGATTCAGC AGCGCCTGGC GCAGTTCGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCA
    GCTGCCCGGG GCCAGCGGCT AGAAGAATCT TTGGAATATC AGCAGTTTGT AGCCAACGTG
    GAGGAGGAGG AAGCCTGGAT CAATGAGAAG ATGACCCTGG TGGCCAGTGA AGATTATGGA
    GACACTCTTG CTGCCATCCA GGGCTTGCTG AAGAAACACG AAGCCTTCGA GACGGACTTC
    ACTGTCCACA AGGACCGAGT GAATGATGTC TGCACCAACG GCCAGGACCT GGTTAAGAAG
    AACAATCACC ATGAGGAGAA CATCTCTTCC AAGATGAAGG GCTTGAACGG GAAAGTGTCA
    GACTTGGAGA AGGCTGCCTC CCAGAGGAAG GCCAAGCTCG ACGAGAACTC GGCCTTCCTC
    CAGTTCAACT GGAAAGCCGA TGTGGTGGAG TCCTGGATCG GTGAAAAGGA GAACAGCTTG
    AAGACAGATG ATTATGGTCG AGATCTGTCT TCTGTCCAAA CTCTCCTCAC CAAACAGGAA
    ACCTTCGATG CTGGCCTGCA GGCCTTCCAG CAGGAGGGCA TCGCCAACAT CACTGCCCTC
    AAAGATCAGC TGCTGGCCGC CAAGCACGTT CAGTCCAAGG CCATCGAGGC CCGCCATGCT
    GCCCTCATGA AGAGGTGGAG CCAGCTCCTG GCCAACTCGG CCACCCGGAA GAAGAAGCTG
    CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAGGATCTCT TCCTGACCTT TGCTAAAAAG
    GCTTCAGCCT TCAACAGCTG GTTTGAAAAC GCAGAGGAGG ACTTAACCGA CCCAGTGCGC
    TGCAACTCTC TGGAAGAGAT CAAGGCCTTG AGAGAGGCCC ACGACGCCTT CCGCTCCTCG
    TTGAGCTCGG CCCAGGCCGA CTTCAACCAG CTGGCAGAGC TGGACCGCCA GATCAAGAGC
    TTCCGCGTGG CCTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG
    AGAAACCTAC AGAAGATCAT CAAGGAAAGG GAGCTGGAGC TGCAGAAGGA GCAGCGACGG
    CAGGAGGAGA ATGACAAGCT GCGCCAGGAG TTCGCCCAGC ATGCCAACGC CTTCCACCAG
    TGGATCCAGG AGACCAGAAC GTACCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT
    GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCC
    GGGACCCTCG AATCACAGCT TGAAGCTACC AAACGCAAAC ACCAGGAGAT TCGAGCTATG
    AGAAGTCAGC TCAAGAAGAT TGAAGACCTG GGGGCGGCCA TGGAGGAAGC GCTGATCCTG
    GACAACAAGT ACACGGAGCA CAGCACCGTG GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC
    CAGTTGGGCA TGCGCATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACGACC
    GGAGTGACCG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC
    AAGTCTGGCC GGCTGAACCA CCAGGAGTTC AAATCCTGTC TGCGCTCCCT GGGCTATGAC
    CTGCCCATGG TGGAGGAAGG GGAGCCCGAC CCTGAGTTCG AGGCCATCCT GGACACTGTG
    GATCCTAACA GGGACGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTTAT GATCAGCCGT
    GAGACCGAGA ACGTCAAGTC CAGCGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCT
    GAAGGGAAGC CTTATGTGAC CAAGGAGGAG CTGTACCAGA ACCTGACTCG GGAACAAGCC
    GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGACGGCA AGGGCCGCGA ACTCCCCACT
    GCTTTTGACT ACGTCGAGTT CACCCGCTCG CTCTTCGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808759.2
    CDS55..7539
    Translation

    Target ORF information:

    RefSeq Version XM_010810457.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810457.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG
    GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGGAGCT GAACGAGCGC TGGCGGTCCC TGCAGCAGCT GGCTGAGGAG
    CGGAGCCAAC TCTTGGGCAG TGCCCATGAA GTGCAGCGGT TCCACAGAGA TGCTGACGAA
    ACCAAAGAGT GGATCGAAGA GAAGAACCAG GCTCTAAACA CAGACAACTA CGGCCACGAC
    CTGGCCAGTG TCCAGGCCCT GCAGCGCAAG CACGAGGGCT TCGAGAGGGA CCTCGCAGCC
    CTTGGTGACA AGGTGAACTC GCTCGGGGAG ACAGCCGAGC GCCTGATCCA GTCCCACCCC
    GAGTCGGCAG AGGACCTGCA GGAGAAGTGC ACTGAGCTGA ACCAGGCATG GAGCAGCCTG
    GGCAAGCGTG CCGACCAGCG CAAGGCCAAG CTGGGCGACT CCCACGACCT GCAGCGCTTC
    CTCAGCGACT TCCGGGACCT CATGTCTTGG ATCAATGGAA TCCGAGGTCT GGTGTCCTCA
    GACGAGCTAG CCAAGGATGT CACCGGAGCT GAGGCCCTGC TGGAGCGACA CCAGGAACAC
    CGGACAGAAA TTGATGCCAG AGCTGGCACT TTCCAGGCAT TCGAGCAGTT TGGGCAGCAG
    CTGTTGGCTC ATGGGCACTA CGCCAGCCCC GAGATCAAGG AGAAGCTCGA TGTTCTAGAC
    CAGGAGCGTG CAGACCTTGA GAAGGCCTGG GTCCAGCGCA GGATGATGCT GGATCAGTGC
    CTTGAATTGC AGCTGTTCCA TCGGGATTGC GAGCAGGCCG AGAACTGGAT GGCTGCCCGA
    GAGGCCTTTC TGAACACAGA AGACAAAGGG GACTCGCTAG ACAGTGTGGA GGCTCTGATC
    AAAAAACACG AGGACTTTGA TAAAGCAATT AATGTCCAGG AGGAGAAGAT TGCTGCCCTG
    CAGTCCTTTG CTGACCAGCT CATCGCTGGT GGCCACTACG CCAAAGGCGA CATCTCCAGC
    CGGCGCAATG AGGTTCTGGA CAGGTGGCGC CGTCTAAAAG CTCAGATGAT TGAGAAGAGG
    TCAAAGCTGG GAGAATCACA GACCCTCCAG CAGTTCAGCC GGGATGTAGA CGAGATTGAA
    GCTTGGATCA GTGAAAAATT ACAGACTGCA AGTGATGAGT CATATAAGGA CCCCACCAAC
    ATCCAGCTTT CCAAGCTGCT GAGCAAGCAT CAGAAGCACC AGGCCTTCGA GGCAGAACTG
    CACGCCAATG CGGACCGGAT CCGTGGGGTC ATCGACATGG GCAACTCCCT CATTGACCGC
    GGGGCCTGCG CCGGCAGCGA GGATGCCGTC AAGGCCCGGC TGGCCGCCTT AGCTGACCAG
    TGGCAGTTCC TGGTGCAGAA GTCGGCTGAG AAGAGCCAGA AGCTGAAAGA AGCCAACAAG
    CAGCAGAACT TCAACACTGG CATCAAGGAC TTCGACTTCT GGCTCTCTGA GGTGGAAGCC
    CTGCTGGCCT CTGAGGATTA TGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTAAAAAAA
    CATCAGCTGC TGGAGGCAGA TATCTCTGCC CACGAGGACC GCCTGAAGGA CCTCAACAGC
    CAGGCGGACA GCCTGATGAC CAGCAGCGCC TTCGACACCT CCCAGGTGAA GGACAAGCGG
    GACGCCATCA ACGGCCGTTT CCAGAAGATC AAGAGCATGG CCGCCTCGAG GCGAGCTAGG
    CTGAATGAGT CCCACCGCCT GCACCAGTTT TTCCGGGACA TGGATGATGA GGAGTCCTGG
    ATCAAGGAAA AGAAGCTGCT GGTGAGCTCA GAGGACTACG GCCGTGACCT GACGGGCGTC
    CAGAACCTGA GGAAGAAGCA CAAACGGCTG GAAGCAGAGC TGGCTGCCCA CGAGCCGGCC
    ATTCAGGGTG TCCTGGACAC TGGAAAGAAG CTGTCTGATG ACAACACCAT CGGGAAGGAG
    GAGATTCAGC AGCGCCTGGC GCAGTTCGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCA
    GCTGCCCGGG GCCAGCGGCT AGAAGAATCT TTGGAATATC AGCAGTTTGT AGCCAACGTG
    GAGGAGGAGG AAGCCTGGAT CAATGAGAAG ATGACCCTGG TGGCCAGTGA AGATTATGGA
    GACACTCTTG CTGCCATCCA GGGCTTGCTG AAGAAACACG AAGCCTTCGA GACGGACTTC
    ACTGTCCACA AGGACCGAGT GAATGATGTC TGCACCAACG GCCAGGACCT GGTTAAGAAG
    AACAATCACC ATGAGGAGAA CATCTCTTCC AAGATGAAGG GCTTGAACGG GAAAGTGTCA
    GACTTGGAGA AGGCTGCCTC CCAGAGGAAG GCCAAGCTCG ACGAGAACTC GGCCTTCCTC
    CAGTTCAACT GGAAAGCCGA TGTGGTGGAG TCCTGGATCG GTGAAAAGGA GAACAGCTTG
    AAGACAGATG ATTATGGTCG AGATCTGTCT TCTGTCCAAA CTCTCCTCAC CAAACAGGAA
    ACCTTCGATG CTGGCCTGCA GGCCTTCCAG CAGGAGGGCA TCGCCAACAT CACTGCCCTC
    AAAGATCAGC TGCTGGCCGC CAAGCACGTT CAGTCCAAGG CCATCGAGGC CCGCCATGCT
    GCCCTCATGA AGAGGTGGAG CCAGCTCCTG GCCAACTCGG CCACCCGGAA GAAGAAGCTG
    CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAGGATCTCT TCCTGACCTT TGCTAAAAAG
    GCTTCAGCCT TCAACAGCTG GTTTGAAAAC GCAGAGGAGG ACTTAACCGA CCCAGTGCGC
    TGCAACTCTC TGGAAGAGAT CAAGGCCTTG AGAGAGGCCC ACGACGCCTT CCGCTCCTCG
    TTGAGCTCGG CCCAGGCCGA CTTCAACCAG CTGGCAGAGC TGGACCGCCA GATCAAGAGC
    TTCCGCGTGG CCTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG
    AGAAACCTAC AGAAGATCAT CAAGGAAAGG GAGCTGGAGC TGCAGAAGGA GCAGCGACGG
    CAGGAGGAGA ATGACAAGCT GCGCCAGGAG TTCGCCCAGC ATGCCAACGC CTTCCACCAG
    TGGATCCAGG AGACCAGAAC GTACCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT
    GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCC
    GGGACCCTCG AATCACAGCT TGAAGCTACC AAACGCAAAC ACCAGGAGAT TCGAGCTATG
    AGAAGTCAGC TCAAGAAGAT TGAAGACCTG GGGGCGGCCA TGGAGGAAGC GCTGATCCTG
    GACAACAAGT ACACGGAGCA CAGCACCGTG GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC
    CAGTTGGGCA TGCGCATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACGACC
    GGAGTGACCG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC
    AAGTCTGGCC GGCTGAACCA CCAGGAGTTC AAATCCTGTC TGCGCTCCCT GGGCTATGAC
    CTGCCCATGG TGGAGGAAGG GGAGCCCGAC CCTGAGTTCG AGGCCATCCT GGACACTGTG
    GATCCTAACA GGGACGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTTAT GATCAGCCGT
    GAGACCGAGA ACGTCAAGTC CAGCGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCT
    GAAGGGAAGC CTTATGTGAC CAAGGAGGAG CTGTACCAGA ACCTGACTCG GGAACAAGCC
    GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGACGGCA AGGGCCGCGA ACTCCCCACT
    GCTTTTGACT ACGTCGAGTT CACCCGCTCG CTCTTCGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92003
    Clone ID Related Accession (Same CDS sequence) XM_010810458.2
    Accession Version XM_010810458.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC
    TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG
    ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC
    CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG
    GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG
    GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC
    GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC
    AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT
    GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA
    GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG
    CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG
    AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG
    GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT
    GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG
    GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA
    GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT
    GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG
    GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC
    GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT
    GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG
    GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC
    CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC
    GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC
    CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG
    AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG
    AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC
    CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG
    CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC
    CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA
    GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT
    GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG
    GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG
    GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG
    AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT
    TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG
    AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC
    AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG
    GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC
    CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC
    TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG
    AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT
    GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG
    ACCAGAACGT ACCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA
    TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC
    AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG
    GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC
    GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT
    TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC
    GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808760.2
    CDS55..7527
    Translation

    Target ORF information:

    RefSeq Version XM_010810458.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810458.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC
    TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG
    ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC
    CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG
    GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG
    GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC
    GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC
    AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT
    GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA
    GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG
    CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG
    AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG
    GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT
    GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG
    GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA
    GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT
    GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG
    GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC
    GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT
    GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG
    GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC
    CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC
    GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC
    CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG
    AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG
    AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC
    CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG
    CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC
    CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA
    GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT
    GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG
    GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG
    GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG
    AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT
    TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG
    AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC
    AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG
    GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC
    CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC
    TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG
    AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT
    GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG
    ACCAGAACGT ACCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTCGAA
    TCACAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCTATGAG AAGTCAGCTC
    AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GTTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACCGG AGTGACCGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCCTGG ACACTGTGGA TCCTAACAGG
    GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAC
    GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCTGA AGGGAAGCCT
    TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTTGACTAC
    GTCGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92004
    Clone ID Related Accession (Same CDS sequence) XM_010810460.2
    Accession Version XM_010810460.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG
    GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC
    TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG
    ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC
    CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG
    GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG
    GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC
    GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC
    AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT
    GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA
    GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG
    CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG
    AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG
    GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT
    GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG
    GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA
    GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT
    GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG
    GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC
    GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT
    GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG
    GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC
    CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC
    GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC
    CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG
    AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG
    AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC
    CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG
    CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC
    CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA
    GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT
    GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG
    GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG
    GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG
    AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT
    TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG
    AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC
    AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG
    GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC
    CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC
    TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG
    AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT
    GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG
    ACCAGGTCCT GCATGGTGGA AGAATCCGGG ACCCTCGAAT CACAGCTTGA AGCTACCAAA
    CGCAAACACC AGGAGATTCG AGCTATGAGA AGTCAGCTCA AGAAGATTGA AGACCTGGGG
    GCGGCCATGG AGGAAGCGCT GATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC
    CTGGCCCAGC AGTGGGACCA GCTCGACCAG TTGGGCATGC GCATGCAGCA CAACCTGGAG
    CAGCAGATCC AGGCCAGGAA CACGACCGGA GTGACCGAGG AGGCCCTCAA AGAATTCAGC
    ATGATGTTCA AACACTTCGA CAAGGACAAG TCTGGCCGGC TGAACCACCA GGAGTTCAAA
    TCCTGTCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA GCCCGACCCT
    GAGTTCGAGG CCATCCTGGA CACTGTGGAT CCTAACAGGG ACGGCCACGT CTCCCTGCAA
    GAGTACATGG CCTTTATGAT CAGCCGTGAG ACCGAGAACG TCAAGTCCAG CGAGGAGATT
    GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA GGGAAGCCTT ATGTGACCAA GGAGGAGCTG
    TACCAGAACC TGACTCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG
    GACGGCAAGG GCCGCGAACT CCCCACTGCT TTTGACTACG TCGAGTTCAC CCGCTCGCTC
    TTCGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808762.2
    CDS54..7505
    Translation

    Target ORF information:

    RefSeq Version XM_010810460.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810460.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
    CTGGAGACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC
    TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT
    TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCA
    GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC
    GAAGCCGAGG TGCAGGCCAA CTCGGGCGCC ATCGTGAAGC TGGATGAGAC TGGTAACCTG
    ATGATCTCAG AAGGGCACTT TGCATCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC
    CGCCAGTGGG AATTGCTCCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC
    CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGATGTGA TGGACTGGAT CAATGACAAG
    GAAGCCATTG TGACGTCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG
    AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG
    AACCAGTTCG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA
    AAGCAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG
    GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATC
    AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC
    AGCGTCCAGG CTTTACTACG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC ATCAGTCCCA CCCTCTGAGT
    GCCACCCAGA TCCAGGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC
    CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCC
    GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA CGCAGATGAA
    CTTGCCAACG ATGTGGCCGG AGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT
    GAAATTGACG CCCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCAGCTGGTC ACTACGCCTC AGATGAAGTG AGGGAGAAGC TTACCGTGCT GTCCGAGGAG
    CGGGCCGCGC TGCTGGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG
    TTCCTGTTGA ACGAAGACCT GGGCGATTCC TTGGATAGTG TGGAAGCGCT TCTAAAGAAG
    CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG
    TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCAATGG AAGATGTGGC CACCCGGCGA
    GACGCACTGC TGAGCCGTCG CAATGCCCTG CATGAGAGAG CCATGTACCG CCGTGCCCAG
    CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG
    GTCAACGAGA AGATGAAAAC AGCCACGGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CGGCAAACCA GAGCCGCATC
    GACGCCCTGG AGAAAGCCGG GCAGAAGCTG ATCGATGTCG ACCACTATGC CAAGGACGAG
    GTGGCGGCTC GGATGAATGA GGTCATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACAGAG
    CTGAAAGGCA TAAAGCTCCG CGAGGCTAAC CAGCAGCAGC AGTTTAATCG CAACGTTGAG
    GACATCGAGC TGTGGCTGTA TGAAGTGGAA GGTCATCTGG CCTCGGACGA CTACGGCAAA
    GACCTCACCA ACGTCCAGAA CCTCCAGAAG AAGCACGCCC TGCTGGAGGC TGACGTGGCA
    GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGACGCGGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCG
    CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCTG ATTCCCTGCG GTTGCAGCAG
    CTCTTCCGTG ACGTCGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC CATTGCCGCC
    TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG
    CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC
    GCCATGGTGG AGGAGGGCCA TTTTGCTGCT GAGGACGTGA AGGCCAAGCT CCATGAGCTG
    AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAC GCCAACGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCGGC CGAGGCTCTG
    CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC
    CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG
    AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGCTT CGTGCCGGCT GCCTACGTGA AGAAGCTGGA TCCTGCGCAG
    TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAACAG
    ATCGACAACC AGACGCGCGT AACTAAGGAG GCTGGCAGCG TGTCTCTGCG TATGAAACAG
    GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG
    AAGAGCTGTA AGAAGTTCAT GTTGTTCCGT GAAGCGAACG AGCTACAGCA GTGGATCAAT
    GAGAAAGAAG CGGCCCTGAC AAGTGAGGAG GTTGGCGCGG ACTTGGAGCA GGTTGAAGTG
    CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC GCGGCTGAAG
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGTC TGATGGCGGA GGAGGTGCAG
    GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA
    GCCTCCCCTT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC
    TTGGGCAGTG CCCATGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG
    ATCGAAGAGA AGAACCAGGC TCTAAACACA GACAACTACG GCCACGACCT GGCCAGTGTC
    CAGGCCCTGC AGCGCAAGCA CGAGGGCTTC GAGAGGGACC TCGCAGCCCT TGGTGACAAG
    GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG
    GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC
    GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC
    AAGGATGTCA CCGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT
    GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGTGCA
    GACCTTGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG
    CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGAGA GGCCTTTCTG
    AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGTGTGGAGG CTCTGATCAA AAAACACGAG
    GACTTTGATA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT
    GACCAGCTCA TCGCTGGTGG CCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG
    GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA
    GAATCACAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT
    GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG
    GACCGGATCC GTGGGGTCAT CGACATGGGC AACTCCCTCA TTGACCGCGG GGCCTGCGCC
    GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT
    GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG
    GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC
    CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ACAAGCGGGA CGCCATCAAC
    GGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCGAGGC GAGCTAGGCT GAATGAGTCC
    CACCGCCTGC ACCAGTTTTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAAAAG
    AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGTGACCTGA CGGGCGTCCA GAACCTGAGG
    AAGAAGCACA AACGGCTGGA AGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC
    CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG
    CGCCTGGCGC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCCCGGGGC
    CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTAG CCAACGTGGA GGAGGAGGAA
    GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAAG ATTATGGAGA CACTCTTGCT
    GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG
    GACCGAGTGA ATGATGTCTG CACCAACGGC CAGGACCTGG TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG
    GCTGCCTCCC AGAGGAAGGC CAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG
    AAAGCCGATG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT
    TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACGTTCA GTCCAAGGCC ATCGAGGCCC GCCATGCTGC CCTCATGAAG
    AGGTGGAGCC AGCTCCTGGC CAACTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CTAAAAAGGC TTCAGCCTTC
    AACAGCTGGT TTGAAAACGC AGAGGAGGAC TTAACCGACC CAGTGCGCTG CAACTCTCTG
    GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GACGCCTTCC GCTCCTCGTT GAGCTCGGCC
    CAGGCCGACT TCAACCAGCT GGCAGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC
    TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG AAACCTACAG
    AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGACGGCA GGAGGAGAAT
    GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG
    ACCAGGTCCT GCATGGTGGA AGAATCCGGG ACCCTCGAAT CACAGCTTGA AGCTACCAAA
    CGCAAACACC AGGAGATTCG AGCTATGAGA AGTCAGCTCA AGAAGATTGA AGACCTGGGG
    GCGGCCATGG AGGAAGCGCT GATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC
    CTGGCCCAGC AGTGGGACCA GCTCGACCAG TTGGGCATGC GCATGCAGCA CAACCTGGAG
    CAGCAGATCC AGGCCAGGAA CACGACCGGA GTGACCGAGG AGGCCCTCAA AGAATTCAGC
    ATGATGTTCA AACACTTCGA CAAGGACAAG TCTGGCCGGC TGAACCACCA GGAGTTCAAA
    TCCTGTCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA GCCCGACCCT
    GAGTTCGAGG CCATCCTGGA CACTGTGGAT CCTAACAGGG ACGGCCACGT CTCCCTGCAA
    GAGTACATGG CCTTTATGAT CAGCCGTGAG ACCGAGAACG TCAAGTCCAG CGAGGAGATT
    GAGAGCGCCT TCCGGGCCCT GAGCTCTGAA GGGAAGCCTT ATGTGACCAA GGAGGAGCTG
    TACCAGAACC TGACTCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG
    GACGGCAAGG GCCGCGAACT CCCCACTGCT TTTGACTACG TCGAGTTCAC CCGCTCGCTC
    TTCGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37323
    Clone ID Related Accession (Same CDS sequence) XM_015473654.1 , XM_024999035.1 , NM_001114156.1
    Accession Version NM_001114156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000011.1. On Feb 1, 2008 this sequence version replaced XM_866848.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571260.49005.1, SRR6764281.2934.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107628.1
    CDS1..7419
    Translation

    Target ORF information:

    RefSeq Version NM_001114156.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001114156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24457
    Clone ID Related Accession (Same CDS sequence) XM_005213345.2 , XM_005213345.3 , XM_024999029.1
    Accession Version XM_005213345.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG
    ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA
    GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG
    GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC
    CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC
    CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT
    ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT
    CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC
    GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213402.1
    CDS122..7618
    Translation

    Target ORF information:

    RefSeq Version XM_005213345.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213345.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG
    ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA
    GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG
    GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC
    CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC
    CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT
    ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT
    CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC
    GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24457
    Clone ID Related Accession (Same CDS sequence) XM_005213345.2 , XM_005213345.3 , XM_024999029.1
    Accession Version XM_005213345.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG
    ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA
    GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG
    GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC
    CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC
    CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT
    ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT
    CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC
    GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213402.1
    CDS121..7617
    Translation

    Target ORF information:

    RefSeq Version XM_005213345.3
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213345.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG
    ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA
    GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG
    GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC
    CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC
    CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT
    ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT
    CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC
    GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24457
    Clone ID Related Accession (Same CDS sequence) XM_005213345.2 , XM_005213345.3 , XM_024999029.1
    Accession Version XM_024999029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG
    ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA
    GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG
    GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC
    CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC
    CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT
    ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT
    CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC
    GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854797.1
    CDS25..7521
    Translation

    Target ORF information:

    RefSeq Version XM_024999029.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT CGAATCACAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG
    ATTCGAGCTA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA
    GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG
    GACCAGCTCG ACCAGTTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACGA CCGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CACCAGGAGT TCAAATCCTG TCTGCGCTCC
    CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC
    CTGGACACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT
    ATGATCAGCC GTGAGACCGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CTGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT
    CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC
    GAACTCCCCA CTGCTTTTGA CTACGTCGAG TTCACCCGCT CGCTCTTCGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37323
    Clone ID Related Accession (Same CDS sequence) XM_015473654.1 , XM_024999035.1 , NM_001114156.1
    Accession Version XM_015473654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329140.1
    CDS26..7444
    Translation

    Target ORF information:

    RefSeq Version XM_015473654.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37323
    Clone ID Related Accession (Same CDS sequence) XM_015473654.1 , XM_024999035.1 , NM_001114156.1
    Accession Version XM_024999035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854803.1
    CDS120..7538
    Translation

    Target ORF information:

    RefSeq Version XM_024999035.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54551
    Clone ID Related Accession (Same CDS sequence) XM_010810459.2 , XM_024999033.1
    Accession Version XM_010810459.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG
    GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT
    CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG
    CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC
    CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG
    GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG
    GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG
    ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC
    CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG
    GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA
    CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808761.1
    CDS26..7459
    Translation

    Target ORF information:

    RefSeq Version XM_010810459.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG
    GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT
    CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG
    CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC
    CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG
    GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG
    GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG
    ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC
    CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG
    GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA
    CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54551
    Clone ID Related Accession (Same CDS sequence) XM_010810459.2 , XM_024999033.1
    Accession Version XM_024999033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG
    GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT
    CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG
    CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC
    CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG
    GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG
    GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG
    ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC
    CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG
    GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA
    CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854801.1
    CDS165..7598
    Translation

    Target ORF information:

    RefSeq Version XM_024999033.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGACGC GCGTAACTAA GGAGGCTGGC
    AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG
    GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT
    CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG
    CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC
    CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG
    GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG
    GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG
    ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC
    CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG
    GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA
    CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54553
    Clone ID Related Accession (Same CDS sequence) XM_010810461.2 , XM_024999040.1
    Accession Version XM_010810461.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG
    GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT
    CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG
    CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC
    CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG
    GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG
    GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG
    ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC
    CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG
    GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA
    CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808763.1
    CDS26..7399
    Translation

    Target ORF information:

    RefSeq Version XM_010810461.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810461.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG
    GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT
    CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG
    CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC
    CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG
    GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG
    GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG
    ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC
    CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG
    GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA
    CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54553
    Clone ID Related Accession (Same CDS sequence) XM_010810461.2 , XM_024999040.1
    Accession Version XM_024999040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG
    GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT
    CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG
    CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC
    CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG
    GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG
    GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG
    ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC
    CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG
    GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA
    CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854808.1
    CDS126..7499
    Translation

    Target ORF information:

    RefSeq Version XM_024999040.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC
    GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGTGGGG TCATCGACAT GGGCAACTCC
    CTCATTGACC GCGGGGCCTG CGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT
    GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG
    GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG
    AAGGACAAGC GGGACGCCAT CAACGGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCG
    AGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TTTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA AAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGTGAC
    CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAAGCAGA GCTGGCTGCC
    CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC
    ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CAGCTGCCCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT
    GTAGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT
    GAAGATTATG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC
    GAGACGGACT TCACTGTCCA CAAGGACCGA GTGAATGATG TCTGCACCAA CGGCCAGGAC
    CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC
    GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCCAAGCT CGACGAGAAC
    TCGGCCTTCC TCCAGTTCAA CTGGAAAGCC GATGTGGTGG AGTCCTGGAT CGGTGAAAAG
    GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC
    ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACG TTCAGTCCAA GGCCATCGAG
    GCCCGCCATG CTGCCCTCAT GAAGAGGTGG AGCCAGCTCC TGGCCAACTC GGCCACCCGG
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC
    TTTGCTAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ACGCAGAGGA GGACTTAACC
    GACCCAGTGC GCTGCAACTC TCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGACGCC
    TTCCGCTCCT CGTTGAGCTC GGCCCAGGCC GACTTCAACC AGCTGGCAGA GCTGGACCGC
    CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACCT GGAGAAACCT ACAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG
    GAGCAGCGAC GGCAGGAGGA GAATGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTACCTCC TCGATGGGTC CTGCATGGTG
    GAAGAATCCG GGACCCTCGA ATCACAGCTT GAAGCTACCA AACGCAAACA CCAGGAGATT
    CGAGCTATGA GAAGTCAGCT CAAGAAGATT GAAGACCTGG GGGCGGCCAT GGAGGAAGCG
    CTGATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTGGCCCA GCAGTGGGAC
    CAGCTCGACC AGTTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACGACCG GAGTGACCGA GGAGGCCCTC AAAGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAC CAGGAGTTCA AATCCTGTCT GCGCTCCCTG
    GGCTATGACC TGCCCATGGT GGAGGAAGGG GAGCCCGACC CTGAGTTCGA GGCCATCCTG
    GACACTGTGG ATCCTAACAG GGACGGCCAC GTCTCCCTGC AAGAGTACAT GGCCTTTATG
    ATCAGCCGTG AGACCGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC
    CTGAGCTCTG AAGGGAAGCC TTATGTGACC AAGGAGGAGC TGTACCAGAA CCTGACTCGG
    GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAA
    CTCCCCACTG CTTTTGACTA CGTCGAGTTC ACCCGCTCGC TCTTCGTGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54554
    Clone ID Related Accession (Same CDS sequence) XM_010810462.2 , XM_024999042.1
    Accession Version XM_010810462.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808764.1
    CDS26..7384
    Translation

    Target ORF information:

    RefSeq Version XM_010810462.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810462.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54554
    Clone ID Related Accession (Same CDS sequence) XM_010810462.2 , XM_024999042.1
    Accession Version XM_024999042.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854810.1
    CDS120..7478
    Translation

    Target ORF information:

    RefSeq Version XM_024999042.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT
    GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAGC TTCAGATTGC CTCAGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC
    AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG CGCCATCGTG
    AAGCTGGATG AGACTGGTAA CCTGATGATC TCAGAAGGGC ACTTTGCATC CGAAACCATC
    CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TCCTGGAGAA GATGCGGGAG
    AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAT
    GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACGT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC
    CACGAAGAGA GAGTCAATGA AGTGAACCAG TTCGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCTGAGGAGG AGCTGATCAA GACAAAGCAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG
    AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT
    AACAGGGATG TGGACGAGAC CATCAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT
    GATGACTTTG GCCGGGACCT GGCCAGCGTC CAGGCTTTAC TACGGAAACA CGAGGGTTTG
    GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC
    CTGCATCAGT CCCACCCTCT GAGTGCCACC CAGATCCAGG TGAAGCGAGA GGAGCTGATC
    ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC
    TACAGGCTTC AGCGCTTCCT TGCCGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG
    AAAGCCCTCA TCAACGCAGA TGAACTTGCC AACGATGTGG CCGGAGCCGA AGCCCTGCTG
    GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCCCATG AAGATAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTACG CCTCAGATGA AGTGAGGGAG
    AAGCTTACCG TGCTGTCCGA GGAGCGGGCC GCGCTGCTGG AGCTCTGGGA GCTGCGACGG
    CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGCGA TTCCTTGGAT
    AGTGTGGAAG CGCTTCTAAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG
    GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCA
    ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GTCGCAATGC CCTGCATGAG
    AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT
    GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC GGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG
    CTCTCGGCAA ACCAGAGCCG CATCGACGCC CTGGAGAAAG CCGGGCAGAA GCTGATCGAT
    GTCGACCACT ATGCCAAGGA CGAGGTGGCG GCTCGGATGA ATGAGGTCAT CAGTCTGTGG
    AAGAAGCTGC TGGAGGCCAC AGAGCTGAAA GGCATAAAGC TCCGCGAGGC TAACCAGCAG
    CAGCAGTTTA ATCGCAACGT TGAGGACATC GAGCTGTGGC TGTATGAAGT GGAAGGTCAT
    CTGGCCTCGG ACGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAGAAGCAC
    GCCCTGCTGG AGGCTGACGT GGCAGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA
    GCCCGCCAGT TCCAGGACGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG
    GCCCTGGTAG CTCGCTACGA GGCGCTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG
    GCTGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTCG AGGATGAGGA GACGTGGATT
    CGGGAGAAAG AACCCATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG
    AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC
    AAAGCAGTCA CACAGAAGGG GAACGCCATG GTGGAGGAGG GCCATTTTGC TGCTGAGGAC
    GTGAAGGCCA AGCTCCATGA GCTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGACGCCAAC
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA
    GATGAGGACT CGGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG
    AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC
    AACAAGGACT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GCTTCGTGCC GGCTGCCTAC
    GTGAAGAAGC TGGATCCTGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC
    AGCATCGCGC TGCGGCAGGA ACAGATCGAC AACCAGTACC ATTCTCTGCT GGAACTGGGA
    GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGTTGTT CCGTGAAGCG
    AACGAGCTAC AGCAGTGGAT CAATGAGAAA GAAGCGGCCC TGACAAGTGA GGAGGTTGGC
    GCGGACTTGG AGCAGGTTGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC
    AAGGCCAATG AGTCGCGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA
    GGTCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC
    AGGGATGAAT CTGATTCCAA GACAGCCTCC CCTTGGAAGT CTGCTCGCCT GATGGTTCAC
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GCTGGCGGTC CCTGCAGCAG
    CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGTGCCCATG AAGTGCAGCG GTTCCACAGA
    GATGCTGACG AAACCAAAGA GTGGATCGAA GAGAAGAACC AGGCTCTAAA CACAGACAAC
    TACGGCCACG ACCTGGCCAG TGTCCAGGCC CTGCAGCGCA AGCACGAGGG CTTCGAGAGG
    GACCTCGCAG CCCTTGGTGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC
    CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA
    TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC
    CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT
    CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCCCT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG
    TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC
    GATGTTCTAG ACCAGGAGCG TGCAGACCTT GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG
    ATGGCTGCCC GAGAGGCCTT TCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGTGTG
    GAGGCTCTGA TCAAAAAACA CGAGGACTTT GATAAAGCAA TTAATGTCCA GGAGGAGAAG
    ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATCGCTG GTGGCCACTA CGCCAAAGGC
    GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG
    ATTGAGAAGA GGTCAAAGCT GGGAGAATCA CAGACCCTCC AGCAGTTCAG CCGGGATGTA
    GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG
    GACCCCACCA ACATCCAGAG CAAGCATCAG AAGCACCAGG CCTTCGAGGC AGAACTGCAC
    GCCAATGCGG ACCGGATCCG TGGGGTCATC GACATGGGCA ACTCCCTCAT TGACCGCGGG
    GCCTGCGCCG GCAGCGAGGA TGCCGTCAAG GCCCGGCTGG CCGCCTTAGC TGACCAGTGG
    CAGTTCCTGG TGCAGAAGTC GGCTGAGAAG AGCCAGAAGC TGAAAGAAGC CAACAAGCAG
    CAGAACTTCA ACACTGGCAT CAAGGACTTC GACTTCTGGC TCTCTGAGGT GGAAGCCCTG
    CTGGCCTCTG AGGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT AAAAAAACAT
    CAGCTGCTGG AGGCAGATAT CTCTGCCCAC GAGGACCGCC TGAAGGACCT CAACAGCCAG
    GCGGACAGCC TGATGACCAG CAGCGCCTTC GACACCTCCC AGGTGAAGGA CAAGCGGGAC
    GCCATCAACG GCCGTTTCCA GAAGATCAAG AGCATGGCCG CCTCGAGGCG AGCTAGGCTG
    AATGAGTCCC ACCGCCTGCA CCAGTTTTTC CGGGACATGG ATGATGAGGA GTCCTGGATC
    AAGGAAAAGA AGCTGCTGGT GAGCTCAGAG GACTACGGCC GTGACCTGAC GGGCGTCCAG
    AACCTGAGGA AGAAGCACAA ACGGCTGGAA GCAGAGCTGG CTGCCCACGA GCCGGCCATT
    CAGGGTGTCC TGGACACTGG AAAGAAGCTG TCTGATGACA ACACCATCGG GAAGGAGGAG
    ATTCAGCAGC GCCTGGCGCA GTTCGTGGAG CACTGGAAGG AGCTGAAGCA GCTGGCAGCT
    GCCCGGGGCC AGCGGCTAGA AGAATCTTTG GAATATCAGC AGTTTGTAGC CAACGTGGAG
    GAGGAGGAAG CCTGGATCAA TGAGAAGATG ACCCTGGTGG CCAGTGAAGA TTATGGAGAC
    ACTCTTGCTG CCATCCAGGG CTTGCTGAAG AAACACGAAG CCTTCGAGAC GGACTTCACT
    GTCCACAAGG ACCGAGTGAA TGATGTCTGC ACCAACGGCC AGGACCTGGT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCCAAG ATGAAGGGCT TGAACGGGAA AGTGTCAGAC
    TTGGAGAAGG CTGCCTCCCA GAGGAAGGCC AAGCTCGACG AGAACTCGGC CTTCCTCCAG
    TTCAACTGGA AAGCCGATGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG
    ACAGATGATT ATGGTCGAGA TCTGTCTTCT GTCCAAACTC TCCTCACCAA ACAGGAAACC
    TTCGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGCATCG CCAACATCAC TGCCCTCAAA
    GATCAGCTGC TGGCCGCCAA GCACGTTCAG TCCAAGGCCA TCGAGGCCCG CCATGCTGCC
    CTCATGAAGA GGTGGAGCCA GCTCCTGGCC AACTCGGCCA CCCGGAAGAA GAAGCTGCTG
    GAGGCACAGA GTCACTTCCG CAAGGTGGAG GATCTCTTCC TGACCTTTGC TAAAAAGGCT
    TCAGCCTTCA ACAGCTGGTT TGAAAACGCA GAGGAGGACT TAACCGACCC AGTGCGCTGC
    AACTCTCTGG AAGAGATCAA GGCCTTGAGA GAGGCCCACG ACGCCTTCCG CTCCTCGTTG
    AGCTCGGCCC AGGCCGACTT CAACCAGCTG GCAGAGCTGG ACCGCCAGAT CAAGAGCTTC
    CGCGTGGCCT CCAACCCCTA CACCTGGTTC ACCATGGAGG CCCTGGAGGA GACCTGGAGA
    AACCTACAGA AGATCATCAA GGAAAGGGAG CTGGAGCTGC AGAAGGAGCA GCGACGGCAG
    GAGGAGAATG ACAAGCTGCG CCAGGAGTTC GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAGA CCAGAACGTA CCTCCTCGAT GGGTCCTGCA TGGTGGAAGA ATCCGGGACC
    CTCGAATCAC AGCTTGAAGC TACCAAACGC AAACACCAGG AGATTCGAGC TATGAGAAGT
    CAGCTCAAGA AGATTGAAGA CCTGGGGGCG GCCATGGAGG AAGCGCTGAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC CGTGGGCCTG GCCCAGCAGT GGGACCAGCT CGACCAGTTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC GACCGGAGTG
    ACCGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCACCAGGA GTTCAAATCC TGTCTGCGCT CCCTGGGCTA TGACCTGCCC
    ATGGTGGAGG AAGGGGAGCC CGACCCTGAG TTCGAGGCCA TCCTGGACAC TGTGGATCCT
    AACAGGGACG GCCACGTCTC CCTGCAAGAG TACATGGCCT TTATGATCAG CCGTGAGACC
    GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTGAG CTCTGAAGGG
    AAGCCTTATG TGACCAAGGA GGAGCTGTAC CAGAACCTGA CTCGGGAACA AGCCGACTAC
    TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAACTCCC CACTGCTTTT
    GACTACGTCG AGTTCACCCG CTCGCTCTTC GTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.