AGL cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGL
Entrez Gene ID	517397
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
General protein information	Preferred Names amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Names glycogen debranching enzyme amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005204113.3	XP_005204170.1	glycogen debranching enzyme isoform X1
XM_005204114.3	XP_005204171.1	glycogen debranching enzyme isoform X1
XM_005204115.3	XP_005204172.1	glycogen debranching enzyme isoform X1
XM_005204115.4	XP_005204172.1	glycogen debranching enzyme isoform X1
XM_005204113.4	XP_005204170.1	glycogen debranching enzyme isoform X1
NM_001192471.1	NP_001179400.1	glycogen debranching enzyme
XM_005204114.4	XP_005204171.1	glycogen debranching enzyme isoform X1
XM_024988818.1	XP_024844586.1	glycogen debranching enzyme isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Homo sapiens (human)	AGL	NP_000635.2
	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa232753	XM_024988818.1 Latest version!	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa17590	NM_001192471.1 Latest version!	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa17590	XM_005204114.4 Latest version!	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa17590	XM_005204115.4 Latest version!	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa17590	XM_005204113.4 Latest version!	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa17590	XM_005204115.3	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa17590	XM_005204113.3	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa17590	XM_005204114.3	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGL ( XM_024988818.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844586.1 Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa232753
Clone ID Related Accession (Same CDS sequence)	XM_024988818.1
Accession Version	XM_024988818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTT TTGATGAATG GGAAAGCAGA CTTATGGTTG CAAAAGAATC AGGCTACAAC 
ATGATTCATT TTACCCCACT GCAGACTCTT GGACTATCCA GGTCGTGCTA TTCCCTTGCT 
GATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTAATA AAAAGTACAC CTGGACTGAT 
GTTGGACAGC TGGTGCAAAA ATTGAAAAAG GAATGGAATA TGCTTTGTAT AACTGATGTT 
GTCTACAATC ATACTGCTGC TAATAGTAGA TGGCTCCAGG AACATCCAGA AAGTGCATAT 
AACCTTGTGA ATTCCCCACA CTTAAAACCT GCCTGGGTCT TAGACAGAGC ACTTTGGCAC 
TTATCCTGTG ATGTTGCAGA AGGGAAATAC AAAGACAGAG GAGTACCTGC TTTGATCGAA 
AATGACCATC AAATGAATTG CATTCGAAAA GTAATTTGGG AGGATATTTT TCCAAAGACT 
CAACTCTGGG AATTTTTCCA AGTAGATGTT CACAAAGCAG TCGAGCAATT TAGAGGACTT 
CTTACACAAG AAAATAGGAA AACAACAAGG CCTGATCCAG ATCACCACCT TAAGATAATT 
CAGGATCCTG AATACAGACG GCTTGGCTGC ACTGTAGATA TGAATGTTGC TCTAGCAACT 
TTCATACCAC ATGACAATGG GCCAGCCGCA ATTAATGAAT GCTGCAATTG GTTCAGGAAG 
AGAATTGAGG AATTAAATTC GGAGAAGCAT CAACTCATTA ACTATCATCA GGAACAGGCA 
GTCAATTGCC TTTTGGGAAA TGTGTTTTAT GAACGACTGG CTGACCATGG ACCTAAACTA 
GGACCTGTTA CTAGAAAACA TCCTTTGGTT ACCAGATATT TTACTTTCCC CTTTGAAGAC 
ATATCCCTCT CTGTAGAAGA ATCAATGATT CATGTCCCAA ATAAAGCTTG TTTTCTGATG 
GCACATAATG GATGGGTAAT GGGAGATGAT CCCCTTCGAA ATTTTGCTGA ACCAGGTTCA 
GATGTTTATC TAAGGAGAGA ACTTATTTGC TGGGGAGACA GTGTTAAATT ACGCTATGGG 
AAAAAACCAG AGGACTGTCC TTATCTCTGG GCACACATGA AAAAATACAC AGAAATCACC 
GCAACTTATT TCCAGGGAGT CCGTCTTGAT AATTGTCACT CAACACCTCT TCATGTAGCA 
GAGTATATGT TGGATGCTGC TAGGAAATTG CAACCCAATT TATATGTAGT AGCTGAACTG 
TTCACAGGAA GTGAAGACCT GGACAATGTC TTTGTTACTA GACTGGGCAT TAGTTCCTTA 
ATAAGAGAGG CAATGAGTGC ATACGATAGC CATGAAGAGG GCAGATTAGT TTACCGATAC 
GGAGGAGAGC CTGTTGGATC CTTTGTTCAG CCCTGTTTGA GGCCTTTAGT GCCAGCTATT 
GCACATGCCC TGTTTATGGA TATCACCCAT GATAACGAGT GTCCTATTGT GCATAGGTCA 
GCATATGACG CTCTCCCAAG TACCACAATT GTCTCCATGG CGTGTTGTGC TAGTGGTAGT 
ACGAAAGGCT ATGACGAATT AGTGCCTCAC CAGATTTCAG TAGTTTCTGA AGAGCGGTTT 
TACACTAAAT GGAATCCTGC AGCATCGCCA TCCAGCGCTG GTGAAGTGAA CTCCCAGAGT 
GGCATTATTG CGGCCAGGTG TGCAATCAAT AAACTTCACC AAGAGCTTGG AGCCAAGGGT 
TTTATCCAGG TGTATGTGGA TCAAGTTGAT GCAGACATAG TGGCTGTAAC CAGACATTCA 
CCCAGTATCC ATCAGTCGGT CGTATCTGTT TCTAGAACTG CTTTCAGGAA TCCCAAGACT 
TCATTTTACA GCAAGGAAGT GCCTCAAATG TGCATCCCTG GCAAAATTGA AGAAGTAGTT 
CTTGAAGCTA GAACTGTTGA GAGAAATACA GCACCTTATA GGAAGGATGC TAATTCAATC 
AATGGAATAC CAAACATCAC AGTAGAAATT AGAGAACATA TTCAGCTCAA TGAAAGTAAA 
ATTGTTAAGC AAGCTGGAGT TACTACAAAA GGACCCAATG AATATATTCA AGAAATAGAA 
TTTGAAAATT TGTCTCCAGG AAGTGTTATT ATATTCAGAG TTAGTCTTGA TCCACATGCG 
CAAGTTGCTG TTGGAATTCT TCGAAATCAT CTGACACAGT TCAGTCCTCA CTTTAAATCT 
GGGAGTCTTG CTGTTGACAA TGCAGATCCT ATATTAAAAA TTCCTTTTGC TTCTATTGCC 
TCTAGATTAA CTTTGGCTGA GCTAAATCAG GTACTTTACC GATGTGAATC AGAAGAAAAA 
GAAGATGGTG GAGGATGTTA TAACATACCA AACTGGTTAC CTCTTAAATA TGCAGGTCTT 
CAAGGATTAA TGTCCGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCCTTC 
TGTGATAATT TGAGATCTGG AGACTGGATG ATTGACTATG TCAGTAACCG ACTTATTTCA 
CGGTCAGGAA CTATTGCTGA AGTTGGTAAA TGGTTGCAGG CTATGTTCTT CTACCTGAAG 
CAGATCCCAC GTTACCTTAT CCCGTGTTAC TTTGATGCTA TATTAATCGG TGCATACACC 
ACTCTTCTGG ATGTAGCATG GAAGCAAATG TCAAGCTTTG TTCAGAATGG TTCTACCTTT 
GTGAAACACC TTTCCTTGGG TTCAGTCCAA ATGTGTGGAG TAGGAAGATT CTCTTCTCTG 
CCACTTCTTT CACCTTCGCT GACGGATGTG CCATGCAGGC TGAATGAGAT CACAAGGGAG 
AAGGAGCAGT GTTGTGTGTC TCTAGCTGCA GGCTTACCTC ACTTCTCTGC TGGTATTTTC 
CGCTGCTGGG GAAGGGATAC TTTTATTGCC CTTAGAGGTC TACTGCTGAT TACTGGACGC 
TATTTGGAGG CCAGGAATAT CATTTTAGCA TTTGCTAGTA CCCTGAGACA TGGCCTCATT 
CCTAATCTCC TGGGTGAAGG AACTCACGCC AGATACAACT GCCGGGATGC TGTGTGGTGG 
TGGCTGCAGT GTATTCAGGA CTATTGTAAA ATGGTTCCAA ATGGGCTGGA CATTCTCAAC 
TGCCCAGTTT CCAGAATGTA TCCTGCAGAC GACTCTGTTC CTTTGTCTGC CGGCACAGTG 
GATCAACCAT TGTTTGAAGT AATACAGGAA GCTATGCAAA GACACATGCA GGGCATACAG 
TTCCGAGAAA GGAATGCTGG TCCACAGATA GATCGGAACA TGAAGGATGA AGGTTTTAAT 
ATTACTGCAG GGGTTGATGA AGAAACAGGA TTTGTTTATG GAGGAAATCG CTTCAATTGC 
GGTACCTGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAAAGG AATCCCAGCC 
ACTCCAAGAG ATGGATCTGC TGTGGAAATT GTGGGCCTGA GTAAATCTGC TGTTCGTTGG 
TTGCTGGAAT TATCCCAAAA AAATATTTTT CCTTATCATT ATGTTACAGT GAAAAGACAC 
GGAAAGCTGG TGAGAGTCTC ATATGATGAA TGGAACAGAA AAATACAAGA GAACTTTGAA 
AAGTTATTTT ATGTGTCAGA AGACCCTTCA GATGTCAGTG AAAAGCATCC TAACCTGGTG 
CACAAGCGGG GCATATACAA AGACAGCTAT GGAGCATCAA GCCCTTGGTG TGACTACCAG 
CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTGGCCCCTG AGCTCTTTAC TACCCAAAAA 
GCATGGAAGG CCTTGGAGAT TGCAGAAAAA AAATTGCTTG GTCCTCTTGG CATGAAAACT 
TTGGATCCAG ATGATATGGT TTACTGTGGA ACTTATGACA ATGCCTTAGA CAATGACAAC 
TATAATCTTG CTAAAGGTTT CAATTACCAC CAAGGACCTG AGTGGTTGTG GCCTGTTGGG 
TATTTTCTTC GTGCAAAATT GTACTTTTCC AGATTGATGG GTCCAGAGAC TACTGCAAAA 
ACTATATTTT TGGTTAAAAA TGTCCTTTCT CGACATTATG TTCATCTTGA GAGATCCCCT 
TGGAAAGGAC TTCCAGAGCT GACCAACGAG AATGGCCAAT ACTGCCCTTT CAGCTGTGAA 
ACACAAGCCT GGTCAATTGC TGCTGTTCTT GAAACACTCT ATGACTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844586.1
CDS	550..4740
Translation	MGPFDEWESRLMVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPNKKYTWTDVGQLVQKLKKEWNMLCITDVVYNHTAANSRWLQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKDRGVPALIENDHQMNCIRKVIWEDIFPKTQLWEFFQVDVHKAVEQFRGLLTQENRKTTRPDPDHHLKIIQDPEYRRLGCTVDMNVALATFIPHDNGPAAINECCNWFRKRIEELNSEKHQLINYHQEQAVNCLLGNVFYERLADHGPKLGPVTRKHPLVTRYFTFPFEDISLSVEESMIHVPNKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGKKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPAASPSSAGEVNSQSGIIAARCAINKLHQELGAKGFIQVYVDQVDADIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTAPYRKDANSINGIPNITVEIREHIQLNESKIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQVLYRCESEEKEDGGGCYNIPNWLPLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGRFSSLPLLSPSLTDVPCRLNEITREKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFASTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGLDILNCPVSRMYPADDSVPLSAGTVDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNKGIPATPRDGSAVEIVGLSKSAVRWLLELSQKNIFPYHYVTVKRHGKLVRVSYDEWNRKIQENFEKLFYVSEDPSDVSEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTQKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGTYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSRLMGPETTAKTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_024988818.1
Organism	Bos taurus(cattle)
Definition	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988818.1

ORF Insert Sequence:

ATGGGACCTT TTGATGAATG GGAAAGCAGA CTTATGGTTG CAAAAGAATC AGGCTACAAC 
ATGATTCATT TTACCCCACT GCAGACTCTT GGACTATCCA GGTCGTGCTA TTCCCTTGCT 
GATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTAATA AAAAGTACAC CTGGACTGAT 
GTTGGACAGC TGGTGCAAAA ATTGAAAAAG GAATGGAATA TGCTTTGTAT AACTGATGTT 
GTCTACAATC ATACTGCTGC TAATAGTAGA TGGCTCCAGG AACATCCAGA AAGTGCATAT 
AACCTTGTGA ATTCCCCACA CTTAAAACCT GCCTGGGTCT TAGACAGAGC ACTTTGGCAC 
TTATCCTGTG ATGTTGCAGA AGGGAAATAC AAAGACAGAG GAGTACCTGC TTTGATCGAA 
AATGACCATC AAATGAATTG CATTCGAAAA GTAATTTGGG AGGATATTTT TCCAAAGACT 
CAACTCTGGG AATTTTTCCA AGTAGATGTT CACAAAGCAG TCGAGCAATT TAGAGGACTT 
CTTACACAAG AAAATAGGAA AACAACAAGG CCTGATCCAG ATCACCACCT TAAGATAATT 
CAGGATCCTG AATACAGACG GCTTGGCTGC ACTGTAGATA TGAATGTTGC TCTAGCAACT 
TTCATACCAC ATGACAATGG GCCAGCCGCA ATTAATGAAT GCTGCAATTG GTTCAGGAAG 
AGAATTGAGG AATTAAATTC GGAGAAGCAT CAACTCATTA ACTATCATCA GGAACAGGCA 
GTCAATTGCC TTTTGGGAAA TGTGTTTTAT GAACGACTGG CTGACCATGG ACCTAAACTA 
GGACCTGTTA CTAGAAAACA TCCTTTGGTT ACCAGATATT TTACTTTCCC CTTTGAAGAC 
ATATCCCTCT CTGTAGAAGA ATCAATGATT CATGTCCCAA ATAAAGCTTG TTTTCTGATG 
GCACATAATG GATGGGTAAT GGGAGATGAT CCCCTTCGAA ATTTTGCTGA ACCAGGTTCA 
GATGTTTATC TAAGGAGAGA ACTTATTTGC TGGGGAGACA GTGTTAAATT ACGCTATGGG 
AAAAAACCAG AGGACTGTCC TTATCTCTGG GCACACATGA AAAAATACAC AGAAATCACC 
GCAACTTATT TCCAGGGAGT CCGTCTTGAT AATTGTCACT CAACACCTCT TCATGTAGCA 
GAGTATATGT TGGATGCTGC TAGGAAATTG CAACCCAATT TATATGTAGT AGCTGAACTG 
TTCACAGGAA GTGAAGACCT GGACAATGTC TTTGTTACTA GACTGGGCAT TAGTTCCTTA 
ATAAGAGAGG CAATGAGTGC ATACGATAGC CATGAAGAGG GCAGATTAGT TTACCGATAC 
GGAGGAGAGC CTGTTGGATC CTTTGTTCAG CCCTGTTTGA GGCCTTTAGT GCCAGCTATT 
GCACATGCCC TGTTTATGGA TATCACCCAT GATAACGAGT GTCCTATTGT GCATAGGTCA 
GCATATGACG CTCTCCCAAG TACCACAATT GTCTCCATGG CGTGTTGTGC TAGTGGTAGT 
ACGAAAGGCT ATGACGAATT AGTGCCTCAC CAGATTTCAG TAGTTTCTGA AGAGCGGTTT 
TACACTAAAT GGAATCCTGC AGCATCGCCA TCCAGCGCTG GTGAAGTGAA CTCCCAGAGT 
GGCATTATTG CGGCCAGGTG TGCAATCAAT AAACTTCACC AAGAGCTTGG AGCCAAGGGT 
TTTATCCAGG TGTATGTGGA TCAAGTTGAT GCAGACATAG TGGCTGTAAC CAGACATTCA 
CCCAGTATCC ATCAGTCGGT CGTATCTGTT TCTAGAACTG CTTTCAGGAA TCCCAAGACT 
TCATTTTACA GCAAGGAAGT GCCTCAAATG TGCATCCCTG GCAAAATTGA AGAAGTAGTT 
CTTGAAGCTA GAACTGTTGA GAGAAATACA GCACCTTATA GGAAGGATGC TAATTCAATC 
AATGGAATAC CAAACATCAC AGTAGAAATT AGAGAACATA TTCAGCTCAA TGAAAGTAAA 
ATTGTTAAGC AAGCTGGAGT TACTACAAAA GGACCCAATG AATATATTCA AGAAATAGAA 
TTTGAAAATT TGTCTCCAGG AAGTGTTATT ATATTCAGAG TTAGTCTTGA TCCACATGCG 
CAAGTTGCTG TTGGAATTCT TCGAAATCAT CTGACACAGT TCAGTCCTCA CTTTAAATCT 
GGGAGTCTTG CTGTTGACAA TGCAGATCCT ATATTAAAAA TTCCTTTTGC TTCTATTGCC 
TCTAGATTAA CTTTGGCTGA GCTAAATCAG GTACTTTACC GATGTGAATC AGAAGAAAAA 
GAAGATGGTG GAGGATGTTA TAACATACCA AACTGGTTAC CTCTTAAATA TGCAGGTCTT 
CAAGGATTAA TGTCCGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCCTTC 
TGTGATAATT TGAGATCTGG AGACTGGATG ATTGACTATG TCAGTAACCG ACTTATTTCA 
CGGTCAGGAA CTATTGCTGA AGTTGGTAAA TGGTTGCAGG CTATGTTCTT CTACCTGAAG 
CAGATCCCAC GTTACCTTAT CCCGTGTTAC TTTGATGCTA TATTAATCGG TGCATACACC 
ACTCTTCTGG ATGTAGCATG GAAGCAAATG TCAAGCTTTG TTCAGAATGG TTCTACCTTT 
GTGAAACACC TTTCCTTGGG TTCAGTCCAA ATGTGTGGAG TAGGAAGATT CTCTTCTCTG 
CCACTTCTTT CACCTTCGCT GACGGATGTG CCATGCAGGC TGAATGAGAT CACAAGGGAG 
AAGGAGCAGT GTTGTGTGTC TCTAGCTGCA GGCTTACCTC ACTTCTCTGC TGGTATTTTC 
CGCTGCTGGG GAAGGGATAC TTTTATTGCC CTTAGAGGTC TACTGCTGAT TACTGGACGC 
TATTTGGAGG CCAGGAATAT CATTTTAGCA TTTGCTAGTA CCCTGAGACA TGGCCTCATT 
CCTAATCTCC TGGGTGAAGG AACTCACGCC AGATACAACT GCCGGGATGC TGTGTGGTGG 
TGGCTGCAGT GTATTCAGGA CTATTGTAAA ATGGTTCCAA ATGGGCTGGA CATTCTCAAC 
TGCCCAGTTT CCAGAATGTA TCCTGCAGAC GACTCTGTTC CTTTGTCTGC CGGCACAGTG 
GATCAACCAT TGTTTGAAGT AATACAGGAA GCTATGCAAA GACACATGCA GGGCATACAG 
TTCCGAGAAA GGAATGCTGG TCCACAGATA GATCGGAACA TGAAGGATGA AGGTTTTAAT 
ATTACTGCAG GGGTTGATGA AGAAACAGGA TTTGTTTATG GAGGAAATCG CTTCAATTGC 
GGTACCTGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAAAGG AATCCCAGCC 
ACTCCAAGAG ATGGATCTGC TGTGGAAATT GTGGGCCTGA GTAAATCTGC TGTTCGTTGG 
TTGCTGGAAT TATCCCAAAA AAATATTTTT CCTTATCATT ATGTTACAGT GAAAAGACAC 
GGAAAGCTGG TGAGAGTCTC ATATGATGAA TGGAACAGAA AAATACAAGA GAACTTTGAA 
AAGTTATTTT ATGTGTCAGA AGACCCTTCA GATGTCAGTG AAAAGCATCC TAACCTGGTG 
CACAAGCGGG GCATATACAA AGACAGCTAT GGAGCATCAA GCCCTTGGTG TGACTACCAG 
CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTGGCCCCTG AGCTCTTTAC TACCCAAAAA 
GCATGGAAGG CCTTGGAGAT TGCAGAAAAA AAATTGCTTG GTCCTCTTGG CATGAAAACT 
TTGGATCCAG ATGATATGGT TTACTGTGGA ACTTATGACA ATGCCTTAGA CAATGACAAC 
TATAATCTTG CTAAAGGTTT CAATTACCAC CAAGGACCTG AGTGGTTGTG GCCTGTTGGG 
TATTTTCTTC GTGCAAAATT GTACTTTTCC AGATTGATGG GTCCAGAGAC TACTGCAAAA 
ACTATATTTT TGGTTAAAAA TGTCCTTTCT CGACATTATG TTCATCTTGA GAGATCCCCT 
TGGAAAGGAC TTCCAGAGCT GACCAACGAG AATGGCCAAT ACTGCCCTTT CAGCTGTGAA 
ACACAAGCCT GGTCAATTGC TGCTGTTCTT GAAACACTCT ATGACTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( NM_001192471.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179400.1 Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.

Price & Availability↑

CloneID	OBa17590
Clone ID Related Accession (Same CDS sequence)	XM_005204113.3 , XM_005204114.3 , XM_005204115.3 , XM_005204115.4 , XM_005204113.4 , NM_001192471.1 , XM_005204114.4
Accession Version	NM_001192471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	glycogen debranching enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000003.1. On Jul 17, 2010 this sequence version replaced XM_595566.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.48805.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179400.1
CDS	64..4662
Translation	MGHSKQIRILLLNEMEKLERTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCMGPFDEWESRLMVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPNKKYTWTDVGQLVQKLKKEWNMLCITDVVYNHTAANSRWLQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKDRGVPALIENDHQMNCIRKVIWEDIFPKTQLWEFFQVDVHKAVEQFRGLLTQENRKTTRPDPDHHLKIIQDPEYRRLGCTVDMNVALATFIPHDNGPAAINECCNWFRKRIEELNSEKHQLINYHQEQAVNCLLGNVFYERLADHGPKLGPVTRKHPLVTRYFTFPFEDISLSVEESMIHVPNKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGKKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPAASPSSAGEVNSQSGIIAARCAINKLHQELGAKGFIQVYVDQVDADIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTAPYRKDANSINGIPNITVEIREHIQLNESKIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQVLYRCESEEKEDGGGCYNIPNWLPLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGRFSSLPLLSPSLTDVPCRLNEITREKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFASTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGLDILNCPVSRMYPADDSVPLSAGTVDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNKGIPATPRDGSAVEIVGLSKSAVRWLLELSQKNIFPYHYVTVKRHGKLVRVSYDEWNRKIQENFEKLFYVSEDPSDVSEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTQKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGTYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSRLMGPETTAKTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	NM_001192471.1
Organism	Bos taurus(cattle)
Definition	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192471.1

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005204113.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204170.1 Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

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CloneID	OBa17590
Clone ID Related Accession (Same CDS sequence)	XM_005204113.3 , XM_005204114.3 , XM_005204115.3 , XM_005204115.4 , XM_005204113.4 , NM_001192471.1 , XM_005204114.4
Accession Version	XM_005204113.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103868.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204170.1
CDS	178..4776
Translation	MGHSKQIRILLLNEMEKLERTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCMGPFDEWESRLMVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPNKKYTWTDVGQLVQKLKKEWNMLCITDVVYNHTAANSRWLQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKDRGVPALIENDHQMNCIRKVIWEDIFPKTQLWEFFQVDVHKAVEQFRGLLTQENRKTTRPDPDHHLKIIQDPEYRRLGCTVDMNVALATFIPHDNGPAAINECCNWFRKRIEELNSEKHQLINYHQEQAVNCLLGNVFYERLADHGPKLGPVTRKHPLVTRYFTFPFEDISLSVEESMIHVPNKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGKKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPAASPSSAGEVNSQSGIIAARCAINKLHQELGAKGFIQVYVDQVDADIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTAPYRKDANSINGIPNITVEIREHIQLNESKIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQVLYRCESEEKEDGGGCYNIPNWLPLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGRFSSLPLLSPSLTDVPCRLNEITREKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFASTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGLDILNCPVSRMYPADDSVPLSAGTVDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNKGIPATPRDGSAVEIVGLSKSAVRWLLELSQKNIFPYHYVTVKRHGKLVRVSYDEWNRKIQENFEKLFYVSEDPSDVSEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTQKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGTYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSRLMGPETTAKTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_005204113.3
Organism	Bos taurus(cattle)
Definition	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204113.3

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005204114.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204171.1 Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

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CloneID	OBa17590
Clone ID Related Accession (Same CDS sequence)	XM_005204113.3 , XM_005204114.3 , XM_005204115.3 , XM_005204115.4 , XM_005204113.4 , NM_001192471.1 , XM_005204114.4
Accession Version	XM_005204114.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103868.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204171.1
CDS	307..4905
Translation	MGHSKQIRILLLNEMEKLERTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCMGPFDEWESRLMVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPNKKYTWTDVGQLVQKLKKEWNMLCITDVVYNHTAANSRWLQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKDRGVPALIENDHQMNCIRKVIWEDIFPKTQLWEFFQVDVHKAVEQFRGLLTQENRKTTRPDPDHHLKIIQDPEYRRLGCTVDMNVALATFIPHDNGPAAINECCNWFRKRIEELNSEKHQLINYHQEQAVNCLLGNVFYERLADHGPKLGPVTRKHPLVTRYFTFPFEDISLSVEESMIHVPNKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGKKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPAASPSSAGEVNSQSGIIAARCAINKLHQELGAKGFIQVYVDQVDADIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTAPYRKDANSINGIPNITVEIREHIQLNESKIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQVLYRCESEEKEDGGGCYNIPNWLPLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGRFSSLPLLSPSLTDVPCRLNEITREKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFASTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGLDILNCPVSRMYPADDSVPLSAGTVDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNKGIPATPRDGSAVEIVGLSKSAVRWLLELSQKNIFPYHYVTVKRHGKLVRVSYDEWNRKIQENFEKLFYVSEDPSDVSEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTQKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGTYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSRLMGPETTAKTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_005204114.3
Organism	Bos taurus(cattle)
Definition	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204114.3

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005204115.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204172.1 Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

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CloneID	OBa17590
Clone ID Related Accession (Same CDS sequence)	XM_005204113.3 , XM_005204114.3 , XM_005204115.3 , XM_005204115.4 , XM_005204113.4 , NM_001192471.1 , XM_005204114.4
Accession Version	XM_005204115.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103868.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204115.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204172.1
CDS	158..4756
Translation	MGHSKQIRILLLNEMEKLERTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCMGPFDEWESRLMVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPNKKYTWTDVGQLVQKLKKEWNMLCITDVVYNHTAANSRWLQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKDRGVPALIENDHQMNCIRKVIWEDIFPKTQLWEFFQVDVHKAVEQFRGLLTQENRKTTRPDPDHHLKIIQDPEYRRLGCTVDMNVALATFIPHDNGPAAINECCNWFRKRIEELNSEKHQLINYHQEQAVNCLLGNVFYERLADHGPKLGPVTRKHPLVTRYFTFPFEDISLSVEESMIHVPNKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGKKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPAASPSSAGEVNSQSGIIAARCAINKLHQELGAKGFIQVYVDQVDADIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTAPYRKDANSINGIPNITVEIREHIQLNESKIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQVLYRCESEEKEDGGGCYNIPNWLPLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGRFSSLPLLSPSLTDVPCRLNEITREKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFASTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGLDILNCPVSRMYPADDSVPLSAGTVDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNKGIPATPRDGSAVEIVGLSKSAVRWLLELSQKNIFPYHYVTVKRHGKLVRVSYDEWNRKIQENFEKLFYVSEDPSDVSEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTQKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGTYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSRLMGPETTAKTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_005204115.3
Organism	Bos taurus(cattle)
Definition	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204115.3

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005204115.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204172.1 Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa17590
Clone ID Related Accession (Same CDS sequence)	XM_005204113.3 , XM_005204114.3 , XM_005204115.3 , XM_005204115.4 , XM_005204113.4 , NM_001192471.1 , XM_005204114.4
Accession Version	XM_005204115.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204115.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204172.1
CDS	156..4754
Translation	MGHSKQIRILLLNEMEKLERTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCMGPFDEWESRLMVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPNKKYTWTDVGQLVQKLKKEWNMLCITDVVYNHTAANSRWLQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKDRGVPALIENDHQMNCIRKVIWEDIFPKTQLWEFFQVDVHKAVEQFRGLLTQENRKTTRPDPDHHLKIIQDPEYRRLGCTVDMNVALATFIPHDNGPAAINECCNWFRKRIEELNSEKHQLINYHQEQAVNCLLGNVFYERLADHGPKLGPVTRKHPLVTRYFTFPFEDISLSVEESMIHVPNKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGKKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPAASPSSAGEVNSQSGIIAARCAINKLHQELGAKGFIQVYVDQVDADIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTAPYRKDANSINGIPNITVEIREHIQLNESKIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQVLYRCESEEKEDGGGCYNIPNWLPLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGRFSSLPLLSPSLTDVPCRLNEITREKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFASTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGLDILNCPVSRMYPADDSVPLSAGTVDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNKGIPATPRDGSAVEIVGLSKSAVRWLLELSQKNIFPYHYVTVKRHGKLVRVSYDEWNRKIQENFEKLFYVSEDPSDVSEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTQKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGTYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSRLMGPETTAKTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_005204115.4
Organism	Bos taurus(cattle)
Definition	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204115.4

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005204113.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204170.1 Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa17590
Clone ID Related Accession (Same CDS sequence)	XM_005204113.3 , XM_005204114.3 , XM_005204115.3 , XM_005204115.4 , XM_005204113.4 , NM_001192471.1 , XM_005204114.4
Accession Version	XM_005204113.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204113.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204170.1
CDS	478..5076
Translation	MGHSKQIRILLLNEMEKLERTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCMGPFDEWESRLMVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPNKKYTWTDVGQLVQKLKKEWNMLCITDVVYNHTAANSRWLQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKDRGVPALIENDHQMNCIRKVIWEDIFPKTQLWEFFQVDVHKAVEQFRGLLTQENRKTTRPDPDHHLKIIQDPEYRRLGCTVDMNVALATFIPHDNGPAAINECCNWFRKRIEELNSEKHQLINYHQEQAVNCLLGNVFYERLADHGPKLGPVTRKHPLVTRYFTFPFEDISLSVEESMIHVPNKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGKKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPAASPSSAGEVNSQSGIIAARCAINKLHQELGAKGFIQVYVDQVDADIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTAPYRKDANSINGIPNITVEIREHIQLNESKIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQVLYRCESEEKEDGGGCYNIPNWLPLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGRFSSLPLLSPSLTDVPCRLNEITREKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFASTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGLDILNCPVSRMYPADDSVPLSAGTVDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNKGIPATPRDGSAVEIVGLSKSAVRWLLELSQKNIFPYHYVTVKRHGKLVRVSYDEWNRKIQENFEKLFYVSEDPSDVSEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTQKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGTYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSRLMGPETTAKTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_005204113.4
Organism	Bos taurus(cattle)
Definition	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204113.4

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005204114.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204171.1 Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

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CloneID	OBa17590
Clone ID Related Accession (Same CDS sequence)	XM_005204113.3 , XM_005204114.3 , XM_005204115.3 , XM_005204115.4 , XM_005204113.4 , NM_001192471.1 , XM_005204114.4
Accession Version	XM_005204114.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204114.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204171.1
CDS	303..4901
Translation	MGHSKQIRILLLNEMEKLERTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCMGPFDEWESRLMVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPNKKYTWTDVGQLVQKLKKEWNMLCITDVVYNHTAANSRWLQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKDRGVPALIENDHQMNCIRKVIWEDIFPKTQLWEFFQVDVHKAVEQFRGLLTQENRKTTRPDPDHHLKIIQDPEYRRLGCTVDMNVALATFIPHDNGPAAINECCNWFRKRIEELNSEKHQLINYHQEQAVNCLLGNVFYERLADHGPKLGPVTRKHPLVTRYFTFPFEDISLSVEESMIHVPNKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGKKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPAASPSSAGEVNSQSGIIAARCAINKLHQELGAKGFIQVYVDQVDADIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTAPYRKDANSINGIPNITVEIREHIQLNESKIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQVLYRCESEEKEDGGGCYNIPNWLPLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGRFSSLPLLSPSLTDVPCRLNEITREKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFASTLRHGLIPNLLGEGTHARYNCRDAVWWWLQCIQDYCKMVPNGLDILNCPVSRMYPADDSVPLSAGTVDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNKGIPATPRDGSAVEIVGLSKSAVRWLLELSQKNIFPYHYVTVKRHGKLVRVSYDEWNRKIQENFEKLFYVSEDPSDVSEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTQKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGTYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSRLMGPETTAKTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_005204114.4
Organism	Bos taurus(cattle)
Definition	Bos taurus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204114.4

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TAGGATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAGG 
ACCCTCTTCA GACTTGAACA AGGGTTTGAA CTGCAGTTCC GATTGGGCCC AACTTTACAG 
GGAAAGGCAG TTACTGTGTA TACAAATTAC CCACTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTCGTGGAC CCTATTTTAC GTGTTGGTGC TGATAATCAC 
GTGTTACCCT TGGACTGTGT TACGCTCCAG ACATTTTTAG CCAAGTGTAT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TATGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCCAGG TCGTGCTATT CCCTTGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAGTACACCT GGACTGATGT TGGACAGCTG 
GTGCAAAAAT TGAAAAAGGA ATGGAATATG CTTTGTATAA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAGATG GCTCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTGAAT 
TCCCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCACTT ATCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGACAGAGGA GTACCTGCTT TGATCGAAAA TGACCATCAA 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGACTCA ACTCTGGGAA 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTC GAGCAATTTA GAGGACTTCT TACACAAGAA 
AATAGGAAAA CAACAAGGCC TGATCCAGAT CACCACCTTA AGATAATTCA GGATCCTGAA 
TACAGACGGC TTGGCTGCAC TGTAGATATG AATGTTGCTC TAGCAACTTT CATACCACAT 
GACAATGGGC CAGCCGCAAT TAATGAATGC TGCAATTGGT TCAGGAAGAG AATTGAGGAA 
TTAAATTCGG AGAAGCATCA ACTCATTAAC TATCATCAGG AACAGGCAGT CAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GACCATGGAC CTAAACTAGG ACCTGTTACT 
AGAAAACATC CTTTGGTTAC CAGATATTTT ACTTTCCCCT TTGAAGACAT ATCCCTCTCT 
GTAGAAGAAT CAATGATTCA TGTCCCAAAT AAAGCTTGTT TTCTGATGGC ACATAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAT TTTGCTGAAC CAGGTTCAGA TGTTTATCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA AAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACAG AAATCACCGC AACTTATTTC 
CAGGGAGTCC GTCTTGATAA TTGTCACTCA ACACCTCTTC ATGTAGCAGA GTATATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ACGATAGCCA TGAAGAGGGC AGATTAGTTT ACCGATACGG AGGAGAGCCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAGTGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TCACCCATGA TAACGAGTGT CCTATTGTGC ATAGGTCAGC ATATGACGCT 
CTCCCAAGTA CCACAATTGT CTCCATGGCG TGTTGTGCTA GTGGTAGTAC GAAAGGCTAT 
GACGAATTAG TGCCTCACCA GATTTCAGTA GTTTCTGAAG AGCGGTTTTA CACTAAATGG 
AATCCTGCAG CATCGCCATC CAGCGCTGGT GAAGTGAACT CCCAGAGTGG CATTATTGCG 
GCCAGGTGTG CAATCAATAA ACTTCACCAA GAGCTTGGAG CCAAGGGTTT TATCCAGGTG 
TATGTGGATC AAGTTGATGC AGACATAGTG GCTGTAACCA GACATTCACC CAGTATCCAT 
CAGTCGGTCG TATCTGTTTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAATACAGC ACCTTATAGG AAGGATGCTA ATTCAATCAA TGGAATACCA 
AACATCACAG TAGAAATTAG AGAACATATT CAGCTCAATG AAAGTAAAAT TGTTAAGCAA 
GCTGGAGTTA CTACAAAAGG ACCCAATGAA TATATTCAAG AAATAGAATT TGAAAATTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTCCTCACT TTAAATCTGG GAGTCTTGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC TAGATTAACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAAAAAGA AGATGGTGGA 
GGATGTTATA ACATACCAAA CTGGTTACCT CTTAAATATG CAGGTCTTCA AGGATTAATG 
TCCGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGATAATTTG 
AGATCTGGAG ACTGGATGAT TGACTATGTC AGTAACCGAC TTATTTCACG GTCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CGTGTTACTT TGATGCTATA TTAATCGGTG CATACACCAC TCTTCTGGAT 
GTAGCATGGA AGCAAATGTC AAGCTTTGTT CAGAATGGTT CTACCTTTGT GAAACACCTT 
TCCTTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAGATTCT CTTCTCTGCC ACTTCTTTCA 
CCTTCGCTGA CGGATGTGCC ATGCAGGCTG AATGAGATCA CAAGGGAGAA GGAGCAGTGT 
TGTGTGTCTC TAGCTGCAGG CTTACCTCAC TTCTCTGCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCCCT TAGAGGTCTA CTGCTGATTA CTGGACGCTA TTTGGAGGCC 
AGGAATATCA TTTTAGCATT TGCTAGTACC CTGAGACATG GCCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTCACGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGACT ATTGTAAAAT GGTTCCAAAT GGGCTGGACA TTCTCAACTG CCCAGTTTCC 
AGAATGTATC CTGCAGACGA CTCTGTTCCT TTGTCTGCCG GCACAGTGGA TCAACCATTG 
TTTGAAGTAA TACAGGAAGC TATGCAAAGA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT TACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGCGG TACCTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAAAGGAA TCCCAGCCAC TCCAAGAGAT 
GGATCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGTTGGTT GCTGGAATTA 
TCCCAAAAAA ATATTTTTCC TTATCATTAT GTTACAGTGA AAAGACACGG AAAGCTGGTG 
AGAGTCTCAT ATGATGAATG GAACAGAAAA ATACAAGAGA ACTTTGAAAA GTTATTTTAT 
GTGTCAGAAG ACCCTTCAGA TGTCAGTGAA AAGCATCCTA ACCTGGTGCA CAAGCGGGGC 
ATATACAAAG ACAGCTATGG AGCATCAAGC CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CCCAAAAAGC ATGGAAGGCC 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCTCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGAAC TTATGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTACCACCA AGGACCTGAG TGGTTGTGGC CTGTTGGGTA TTTTCTTCGT 
GCAAAATTGT ACTTTTCCAG ATTGATGGGT CCAGAGACTA CTGCAAAAAC TATATTTTTG 
GTTAAAAATG TCCTTTCTCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAGCTGA CCAACGAGAA TGGCCAATAC TGCCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTG CTGTTCTTGA AACACTCTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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AGL cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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