Agl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Agl
Entrez Gene ID	77559
Full Name	amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Synonyms	1110061O17Rik,9430004C13Rik,9630046L06Rik,AI850929,C77197
General protein information	Preferred Names amylo-1,6-glucosidase, 4-alpha-glucanotransferase Names glycogen debranching enzyme
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 G1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011240280.3	XP_011238582.1	glycogen debranching enzyme isoform X2
NM_001081326.1	NP_001074795.1	glycogen debranching enzyme isoform 1
XM_011240280.2	XP_011238582.1	glycogen debranching enzyme isoform X2
NM_001362367.1	NP_001349296.1	glycogen debranching enzyme isoform 2
XM_006502287.1	XP_006502350.1	glycogen debranching enzyme isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Homo sapiens (human)	AGL	NP_000635.2
	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Gene Ontology
Bibliography

Related articles in PubMed

Sporadic Hirschsprung Disease: Mutational Spectrum and Novel Candidate Genes Revealed by Next-generation Sequencing.
Zhang Z, Li Q, Diao M, Liu N, Cheng W, Xiao P, Zou J, Su L, Yu K, Wu J, Li L, Jiang Q
Scientific reports7(1)14796(2017 Nov)

?H NMR metabolic profiling of plasma reveals additional phenotypes in knockout mouse models.
Probert F, Rice P, Scudamore CL, Wells S, Williams R, Hough TA, Cox IJ
Journal of proteome research14(5)2036-45(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Agl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Agl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu71627	XM_011240280.3 Latest version!	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu83950	NM_001362367.1 Latest version!	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu02123	NM_001081326.1 Latest version!	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu02123	XM_006502287.1 Latest version!	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu71627	XM_011240280.2	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Agl ( XM_011240280.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238582.1 Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu71627
Clone ID Related Accession (Same CDS sequence)	XM_011240280.3 , XM_011240280.2
Accession Version	XM_011240280.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240280.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGATG CTGCTAGGAA ATTACAGCCC AATCTTTATG TAGTGGCTGA GCTGTTCACA 
GGAAGCGAAG AGCTGGACAA TATCTTCGTT ACTAGACTGG GCATCAGTTC CTTAATAAGA 
GAGGCAATGA GCGCGTATAA CAGTCACGAA GAGGGCAGAC TGGTCTACCG GTACGGAGGG 
GAACCTGTCG GCTCCTTCGT TCAGCCCTGT TTGAGGCCGC TCATGCCTGC TATCGCGCAT 
GCCCTGTTTA TGGACATCAC CCACGATAAT GAGTGTCCGA TTGTGCACAG ATCAGCCTAT 
GATGCTCTTC CAAGTACCAC AGTTGTTTCC ATGGCATGCT GTGCCAGTGG AAGCACAAGA 
GGCTACGACG AGCTAGTGCC TCATCAGATT TCAGTGGTTG CAGAGGAGCG ATTTTACACT 
AAGTGGAATC CTGGGGCATC ACCGGCAGAT ACAGGGGATG TTAATGTCCA TAGCGGGATT 
ATTGCAGCCA GATGTGCCAT CAATAGGCTC CATCAAGAGC TTGGAGCCAA GGGTTTCATT 
CAAGTGTATG TGGATCAAGT GGATGAAGAC ATAGTGGCTG TAACCAGGCA CTCCCCGAGC 
ATCCACCAGT CTGTTGTGGC TGTATCGAGA ACTGCTTTTA GGAATCCCAA GACTTCCTTT 
TACAGCAAAG AAGTGCCTCA GATGTGCATC CCTGGCAAAA TTGAAGAGGT AGTTCTGGAA 
GCTAGAACTA TTGAAAGGAA CACAAAACCT TATAAGAAAG ACGAGAACTC GATCAATGGA 
ATGCCGAACA TGACAGTGGA ACTTAGAGAA CACATCCAGC TTCATGAAAG TAAAATTGTC 
AGACAAGCTG GAGTTGCCAC AAAGGGTCCC AATGAATATA TTCAGGAAAT AGAATTTGAG 
AACCTATCTC CAGGAAGTGT TATTATATTC AGAGTTAGTC TCGATCCACA CGCTCAAGTT 
GCTGTGGGGA TTCTTCGGAA CCACCTGACC CAGTTCAGCT CTCACTTTAA GTCCGGAAGC 
CTTGCTGTTG ACAACGCAGA TCCTATATTA AAAATCCCCT TCGCTTCCAT TGCCTCTAAA 
TTGACTTTGG CTGAGCTAAA CCAGGTACTT TACCGATGTG AATCAGAAGA ACAAGAAGAC 
GGTGGCGGTT GCTATGACAT ACCAAACTGG TCATCACTTA AGTACGCAGG ACTTCAAGGA 
TTAATGTCCG TGTTGGCAGA AATAAGACCG AAGAATGACC TGGGGCATCC ATTCTGTGAA 
AACTTGAGGT CGGGAGACTG GATGATCGAC TACGTGAGTG GTCGGCTTAT TTCAAGATCA 
GGAAGTATTG CTGAGGTTGG TAAATGGCTG CAGGCTATGT TCTTCTACCT GAAGCAGATC 
CCACGCTACC TCATCCCCTG CTACTTTGAC GCTATATTGA TTGGTGCATA CACCACTCTG 
CTGGATGTAG CGTGGAAACA GATGTCAAGC TTTGTTCAGA ACGGATCCAC CTTCGTGAAG 
CACCTGTCAC TGGGTTCTGT CCAGATGTGT GGAGTCGGGA AATGCCCTTG TCTGCCGCTG 
CTTTCTCCCT CGCTCCTGGA TGTGCCGTGC AGGTTAAATG AGATCACAAA GGAGAAGGAG 
CAGTGCTGTG CCTCCTTAGC TGCAGGCTTA CCTCACTTTT CTTCCGGTCT TTTTCGCTGC 
TGGGGGAGGG ACACTTTCAT TGCCCTTCGA GGGATGCTGC TGGTTACTGG ACGCTACCTG 
GAAGCCAGGA ACATCATTTT AGCGTTTGCT AGTACCCTGC GGCACGGTCT CATCCCTAAC 
CTCCTCGGCG AAGGGACATA TGCCAGGTAC AACTGCCGGG ACGCCGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATTG CAGGACAGTT CCAAACGGCC TGGACATCCT CAAATGCCCG 
GTGTCCAGGA TGTATCCAAC AGATGACTCC GCACCCTTGC CTGCTGGGAC ACTGGACCAG 
CCTTTGTTTG AAGTCATCCA GGAAGCTATG CAGAGGCACA TGCAGGGCAT CCAGTTCCGA 
GAGAGGAACG CCGGTCCACA GATCGACCGA AACATGAAGG ACGAGGGTTT CAACATAACT 
GCGGGGATTG ATGAAGAAAC AGGATTTGTT TATGGAGGAA ATCGCTTCAA CTGTGGCACG 
TGGATGGATA AAATGGGAGA GAGTGACCGA GCTAGGAACA GAGGGATCCC AGCCACTCCA 
AGAGATGGGT CTGCTGTGGA AATCGTGGGC TTGTGTAAAT CTGCTGTTCG TTGGTTGCTG 
GAATTGTCTA AGAAAAATAT CTTTCCTTAT CATGAAGTCA GAGTAAAAAG ACATGGAAAG 
GTTGTGGCAG TCTCGTACGA TGAGTGGAAC AGAAAAATAC AAAACAATTT CGAAAAGCTG 
TTTCATGTTT CCGAAGACCC TTCAGATCCT AACGAGAAGC ATCCGAACCT GGTTCACAAA 
CGCGGCATCT ACAAGGACAG CTATGGTGCG TCGAGTCCTT GGTGTGACTA TCAGCTCAGG 
CCCAACTTCA CCATAGCAAT GGTCGTGGCC CCTGAACTCT TTACTGCTGA GAAGGCTTGG 
AAGGCTTTGG AAATTGCAGA AAAAAAATTG CTTGGTCCCC TTGGCATGAA AACTTTAGAC 
CCAGATGACA TGGTTTACTG TGGAGTTTAT GATAATGCCT TAGACAATGA CAACTACAAC 
CTTGCTAGAG GCTTCAATTA CCACCAAGGA CCTGAGTGGC TGTGGCCAAT TGGGTATTTT 
CTTCGTGCAA AGTTGTATTT TTCCAAAATG ATGGGTCCAG AGACTGCTGC GAAGACTGTA 
TTTTTGGTTA AAAATGTTCT TTCTAGACAT TATGTTCATC TTGAGAGATC CCCATGGAAA 
GGCCTTCCGG AGCTCACCAA TGAGAACGGC CAGTACTGCC CTTTCAGCTG TGAGACACAG 
GCGTGGTCAA TGGCTGTTGT TCTGGAAACC CTCTATGATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238582.1
CDS	151..3135
Translation	MLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNPGASPADTGDVNVHSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYKKDENSINGMPNMTVELREHIQLHESKIVRQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNADPILKIPFASIASKLTLAELNQVLYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSGRLISRSGSIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEARNIILAFASTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCRTVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGIDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVKRHGKVVAVSYDEWNRKIQNNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKMMGPETAAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSMAVVLETLYDL

Target ORF information:

RefSeq Version	XM_011240280.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240280.2

ORF Insert Sequence:

ATGCTGGATG CTGCTAGGAA ATTACAGCCC AATCTTTATG TAGTGGCTGA GCTGTTCACA 
GGAAGCGAAG AGCTGGACAA TATCTTCGTT ACTAGACTGG GCATCAGTTC CTTAATAAGA 
GAGGCAATGA GCGCGTATAA CAGTCACGAA GAGGGCAGAC TGGTCTACCG GTACGGAGGG 
GAACCTGTCG GCTCCTTCGT TCAGCCCTGT TTGAGGCCGC TCATGCCTGC TATCGCGCAT 
GCCCTGTTTA TGGACATCAC CCACGATAAT GAGTGTCCGA TTGTGCACAG ATCAGCCTAT 
GATGCTCTTC CAAGTACCAC AGTTGTTTCC ATGGCATGCT GTGCCAGTGG AAGCACAAGA 
GGCTACGACG AGCTAGTGCC TCATCAGATT TCAGTGGTTG CAGAGGAGCG ATTTTACACT 
AAGTGGAATC CTGGGGCATC ACCGGCAGAT ACAGGGGATG TTAATGTCCA TAGCGGGATT 
ATTGCAGCCA GATGTGCCAT CAATAGGCTC CATCAAGAGC TTGGAGCCAA GGGTTTCATT 
CAAGTGTATG TGGATCAAGT GGATGAAGAC ATAGTGGCTG TAACCAGGCA CTCCCCGAGC 
ATCCACCAGT CTGTTGTGGC TGTATCGAGA ACTGCTTTTA GGAATCCCAA GACTTCCTTT 
TACAGCAAAG AAGTGCCTCA GATGTGCATC CCTGGCAAAA TTGAAGAGGT AGTTCTGGAA 
GCTAGAACTA TTGAAAGGAA CACAAAACCT TATAAGAAAG ACGAGAACTC GATCAATGGA 
ATGCCGAACA TGACAGTGGA ACTTAGAGAA CACATCCAGC TTCATGAAAG TAAAATTGTC 
AGACAAGCTG GAGTTGCCAC AAAGGGTCCC AATGAATATA TTCAGGAAAT AGAATTTGAG 
AACCTATCTC CAGGAAGTGT TATTATATTC AGAGTTAGTC TCGATCCACA CGCTCAAGTT 
GCTGTGGGGA TTCTTCGGAA CCACCTGACC CAGTTCAGCT CTCACTTTAA GTCCGGAAGC 
CTTGCTGTTG ACAACGCAGA TCCTATATTA AAAATCCCCT TCGCTTCCAT TGCCTCTAAA 
TTGACTTTGG CTGAGCTAAA CCAGGTACTT TACCGATGTG AATCAGAAGA ACAAGAAGAC 
GGTGGCGGTT GCTATGACAT ACCAAACTGG TCATCACTTA AGTACGCAGG ACTTCAAGGA 
TTAATGTCCG TGTTGGCAGA AATAAGACCG AAGAATGACC TGGGGCATCC ATTCTGTGAA 
AACTTGAGGT CGGGAGACTG GATGATCGAC TACGTGAGTG GTCGGCTTAT TTCAAGATCA 
GGAAGTATTG CTGAGGTTGG TAAATGGCTG CAGGCTATGT TCTTCTACCT GAAGCAGATC 
CCACGCTACC TCATCCCCTG CTACTTTGAC GCTATATTGA TTGGTGCATA CACCACTCTG 
CTGGATGTAG CGTGGAAACA GATGTCAAGC TTTGTTCAGA ACGGATCCAC CTTCGTGAAG 
CACCTGTCAC TGGGTTCTGT CCAGATGTGT GGAGTCGGGA AATGCCCTTG TCTGCCGCTG 
CTTTCTCCCT CGCTCCTGGA TGTGCCGTGC AGGTTAAATG AGATCACAAA GGAGAAGGAG 
CAGTGCTGTG CCTCCTTAGC TGCAGGCTTA CCTCACTTTT CTTCCGGTCT TTTTCGCTGC 
TGGGGGAGGG ACACTTTCAT TGCCCTTCGA GGGATGCTGC TGGTTACTGG ACGCTACCTG 
GAAGCCAGGA ACATCATTTT AGCGTTTGCT AGTACCCTGC GGCACGGTCT CATCCCTAAC 
CTCCTCGGCG AAGGGACATA TGCCAGGTAC AACTGCCGGG ACGCCGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATTG CAGGACAGTT CCAAACGGCC TGGACATCCT CAAATGCCCG 
GTGTCCAGGA TGTATCCAAC AGATGACTCC GCACCCTTGC CTGCTGGGAC ACTGGACCAG 
CCTTTGTTTG AAGTCATCCA GGAAGCTATG CAGAGGCACA TGCAGGGCAT CCAGTTCCGA 
GAGAGGAACG CCGGTCCACA GATCGACCGA AACATGAAGG ACGAGGGTTT CAACATAACT 
GCGGGGATTG ATGAAGAAAC AGGATTTGTT TATGGAGGAA ATCGCTTCAA CTGTGGCACG 
TGGATGGATA AAATGGGAGA GAGTGACCGA GCTAGGAACA GAGGGATCCC AGCCACTCCA 
AGAGATGGGT CTGCTGTGGA AATCGTGGGC TTGTGTAAAT CTGCTGTTCG TTGGTTGCTG 
GAATTGTCTA AGAAAAATAT CTTTCCTTAT CATGAAGTCA GAGTAAAAAG ACATGGAAAG 
GTTGTGGCAG TCTCGTACGA TGAGTGGAAC AGAAAAATAC AAAACAATTT CGAAAAGCTG 
TTTCATGTTT CCGAAGACCC TTCAGATCCT AACGAGAAGC ATCCGAACCT GGTTCACAAA 
CGCGGCATCT ACAAGGACAG CTATGGTGCG TCGAGTCCTT GGTGTGACTA TCAGCTCAGG 
CCCAACTTCA CCATAGCAAT GGTCGTGGCC CCTGAACTCT TTACTGCTGA GAAGGCTTGG 
AAGGCTTTGG AAATTGCAGA AAAAAAATTG CTTGGTCCCC TTGGCATGAA AACTTTAGAC 
CCAGATGACA TGGTTTACTG TGGAGTTTAT GATAATGCCT TAGACAATGA CAACTACAAC 
CTTGCTAGAG GCTTCAATTA CCACCAAGGA CCTGAGTGGC TGTGGCCAAT TGGGTATTTT 
CTTCGTGCAA AGTTGTATTT TTCCAAAATG ATGGGTCCAG AGACTGCTGC GAAGACTGTA 
TTTTTGGTTA AAAATGTTCT TTCTAGACAT TATGTTCATC TTGAGAGATC CCCATGGAAA 
GGCCTTCCGG AGCTCACCAA TGAGAACGGC CAGTACTGCC CTTTCAGCTG TGAGACACAG 
GCGTGGTCAA TGGCTGTTGT TCTGGAAACC CTCTATGATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( XM_011240280.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238582.1 Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu71627
Clone ID Related Accession (Same CDS sequence)	XM_011240280.3 , XM_011240280.2
Accession Version	XM_011240280.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGATG CTGCTAGGAA ATTACAGCCC AATCTTTATG TAGTGGCTGA GCTGTTCACA 
GGAAGCGAAG AGCTGGACAA TATCTTCGTT ACTAGACTGG GCATCAGTTC CTTAATAAGA 
GAGGCAATGA GCGCGTATAA CAGTCACGAA GAGGGCAGAC TGGTCTACCG GTACGGAGGG 
GAACCTGTCG GCTCCTTCGT TCAGCCCTGT TTGAGGCCGC TCATGCCTGC TATCGCGCAT 
GCCCTGTTTA TGGACATCAC CCACGATAAT GAGTGTCCGA TTGTGCACAG ATCAGCCTAT 
GATGCTCTTC CAAGTACCAC AGTTGTTTCC ATGGCATGCT GTGCCAGTGG AAGCACAAGA 
GGCTACGACG AGCTAGTGCC TCATCAGATT TCAGTGGTTG CAGAGGAGCG ATTTTACACT 
AAGTGGAATC CTGGGGCATC ACCGGCAGAT ACAGGGGATG TTAATGTCCA TAGCGGGATT 
ATTGCAGCCA GATGTGCCAT CAATAGGCTC CATCAAGAGC TTGGAGCCAA GGGTTTCATT 
CAAGTGTATG TGGATCAAGT GGATGAAGAC ATAGTGGCTG TAACCAGGCA CTCCCCGAGC 
ATCCACCAGT CTGTTGTGGC TGTATCGAGA ACTGCTTTTA GGAATCCCAA GACTTCCTTT 
TACAGCAAAG AAGTGCCTCA GATGTGCATC CCTGGCAAAA TTGAAGAGGT AGTTCTGGAA 
GCTAGAACTA TTGAAAGGAA CACAAAACCT TATAAGAAAG ACGAGAACTC GATCAATGGA 
ATGCCGAACA TGACAGTGGA ACTTAGAGAA CACATCCAGC TTCATGAAAG TAAAATTGTC 
AGACAAGCTG GAGTTGCCAC AAAGGGTCCC AATGAATATA TTCAGGAAAT AGAATTTGAG 
AACCTATCTC CAGGAAGTGT TATTATATTC AGAGTTAGTC TCGATCCACA CGCTCAAGTT 
GCTGTGGGGA TTCTTCGGAA CCACCTGACC CAGTTCAGCT CTCACTTTAA GTCCGGAAGC 
CTTGCTGTTG ACAACGCAGA TCCTATATTA AAAATCCCCT TCGCTTCCAT TGCCTCTAAA 
TTGACTTTGG CTGAGCTAAA CCAGGTACTT TACCGATGTG AATCAGAAGA ACAAGAAGAC 
GGTGGCGGTT GCTATGACAT ACCAAACTGG TCATCACTTA AGTACGCAGG ACTTCAAGGA 
TTAATGTCCG TGTTGGCAGA AATAAGACCG AAGAATGACC TGGGGCATCC ATTCTGTGAA 
AACTTGAGGT CGGGAGACTG GATGATCGAC TACGTGAGTG GTCGGCTTAT TTCAAGATCA 
GGAAGTATTG CTGAGGTTGG TAAATGGCTG CAGGCTATGT TCTTCTACCT GAAGCAGATC 
CCACGCTACC TCATCCCCTG CTACTTTGAC GCTATATTGA TTGGTGCATA CACCACTCTG 
CTGGATGTAG CGTGGAAACA GATGTCAAGC TTTGTTCAGA ACGGATCCAC CTTCGTGAAG 
CACCTGTCAC TGGGTTCTGT CCAGATGTGT GGAGTCGGGA AATGCCCTTG TCTGCCGCTG 
CTTTCTCCCT CGCTCCTGGA TGTGCCGTGC AGGTTAAATG AGATCACAAA GGAGAAGGAG 
CAGTGCTGTG CCTCCTTAGC TGCAGGCTTA CCTCACTTTT CTTCCGGTCT TTTTCGCTGC 
TGGGGGAGGG ACACTTTCAT TGCCCTTCGA GGGATGCTGC TGGTTACTGG ACGCTACCTG 
GAAGCCAGGA ACATCATTTT AGCGTTTGCT AGTACCCTGC GGCACGGTCT CATCCCTAAC 
CTCCTCGGCG AAGGGACATA TGCCAGGTAC AACTGCCGGG ACGCCGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATTG CAGGACAGTT CCAAACGGCC TGGACATCCT CAAATGCCCG 
GTGTCCAGGA TGTATCCAAC AGATGACTCC GCACCCTTGC CTGCTGGGAC ACTGGACCAG 
CCTTTGTTTG AAGTCATCCA GGAAGCTATG CAGAGGCACA TGCAGGGCAT CCAGTTCCGA 
GAGAGGAACG CCGGTCCACA GATCGACCGA AACATGAAGG ACGAGGGTTT CAACATAACT 
GCGGGGATTG ATGAAGAAAC AGGATTTGTT TATGGAGGAA ATCGCTTCAA CTGTGGCACG 
TGGATGGATA AAATGGGAGA GAGTGACCGA GCTAGGAACA GAGGGATCCC AGCCACTCCA 
AGAGATGGGT CTGCTGTGGA AATCGTGGGC TTGTGTAAAT CTGCTGTTCG TTGGTTGCTG 
GAATTGTCTA AGAAAAATAT CTTTCCTTAT CATGAAGTCA GAGTAAAAAG ACATGGAAAG 
GTTGTGGCAG TCTCGTACGA TGAGTGGAAC AGAAAAATAC AAAACAATTT CGAAAAGCTG 
TTTCATGTTT CCGAAGACCC TTCAGATCCT AACGAGAAGC ATCCGAACCT GGTTCACAAA 
CGCGGCATCT ACAAGGACAG CTATGGTGCG TCGAGTCCTT GGTGTGACTA TCAGCTCAGG 
CCCAACTTCA CCATAGCAAT GGTCGTGGCC CCTGAACTCT TTACTGCTGA GAAGGCTTGG 
AAGGCTTTGG AAATTGCAGA AAAAAAATTG CTTGGTCCCC TTGGCATGAA AACTTTAGAC 
CCAGATGACA TGGTTTACTG TGGAGTTTAT GATAATGCCT TAGACAATGA CAACTACAAC 
CTTGCTAGAG GCTTCAATTA CCACCAAGGA CCTGAGTGGC TGTGGCCAAT TGGGTATTTT 
CTTCGTGCAA AGTTGTATTT TTCCAAAATG ATGGGTCCAG AGACTGCTGC GAAGACTGTA 
TTTTTGGTTA AAAATGTTCT TTCTAGACAT TATGTTCATC TTGAGAGATC CCCATGGAAA 
GGCCTTCCGG AGCTCACCAA TGAGAACGGC CAGTACTGCC CTTTCAGCTG TGAGACACAG 
GCGTGGTCAA TGGCTGTTGT TCTGGAAACC CTCTATGATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238582.1
CDS	162..3146
Translation	MLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNPGASPADTGDVNVHSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYKKDENSINGMPNMTVELREHIQLHESKIVRQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNADPILKIPFASIASKLTLAELNQVLYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSGRLISRSGSIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEARNIILAFASTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCRTVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGIDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVKRHGKVVAVSYDEWNRKIQNNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKMMGPETAAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSMAVVLETLYDL

Target ORF information:

RefSeq Version	XM_011240280.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240280.3

ORF Insert Sequence:

ATGCTGGATG CTGCTAGGAA ATTACAGCCC AATCTTTATG TAGTGGCTGA GCTGTTCACA 
GGAAGCGAAG AGCTGGACAA TATCTTCGTT ACTAGACTGG GCATCAGTTC CTTAATAAGA 
GAGGCAATGA GCGCGTATAA CAGTCACGAA GAGGGCAGAC TGGTCTACCG GTACGGAGGG 
GAACCTGTCG GCTCCTTCGT TCAGCCCTGT TTGAGGCCGC TCATGCCTGC TATCGCGCAT 
GCCCTGTTTA TGGACATCAC CCACGATAAT GAGTGTCCGA TTGTGCACAG ATCAGCCTAT 
GATGCTCTTC CAAGTACCAC AGTTGTTTCC ATGGCATGCT GTGCCAGTGG AAGCACAAGA 
GGCTACGACG AGCTAGTGCC TCATCAGATT TCAGTGGTTG CAGAGGAGCG ATTTTACACT 
AAGTGGAATC CTGGGGCATC ACCGGCAGAT ACAGGGGATG TTAATGTCCA TAGCGGGATT 
ATTGCAGCCA GATGTGCCAT CAATAGGCTC CATCAAGAGC TTGGAGCCAA GGGTTTCATT 
CAAGTGTATG TGGATCAAGT GGATGAAGAC ATAGTGGCTG TAACCAGGCA CTCCCCGAGC 
ATCCACCAGT CTGTTGTGGC TGTATCGAGA ACTGCTTTTA GGAATCCCAA GACTTCCTTT 
TACAGCAAAG AAGTGCCTCA GATGTGCATC CCTGGCAAAA TTGAAGAGGT AGTTCTGGAA 
GCTAGAACTA TTGAAAGGAA CACAAAACCT TATAAGAAAG ACGAGAACTC GATCAATGGA 
ATGCCGAACA TGACAGTGGA ACTTAGAGAA CACATCCAGC TTCATGAAAG TAAAATTGTC 
AGACAAGCTG GAGTTGCCAC AAAGGGTCCC AATGAATATA TTCAGGAAAT AGAATTTGAG 
AACCTATCTC CAGGAAGTGT TATTATATTC AGAGTTAGTC TCGATCCACA CGCTCAAGTT 
GCTGTGGGGA TTCTTCGGAA CCACCTGACC CAGTTCAGCT CTCACTTTAA GTCCGGAAGC 
CTTGCTGTTG ACAACGCAGA TCCTATATTA AAAATCCCCT TCGCTTCCAT TGCCTCTAAA 
TTGACTTTGG CTGAGCTAAA CCAGGTACTT TACCGATGTG AATCAGAAGA ACAAGAAGAC 
GGTGGCGGTT GCTATGACAT ACCAAACTGG TCATCACTTA AGTACGCAGG ACTTCAAGGA 
TTAATGTCCG TGTTGGCAGA AATAAGACCG AAGAATGACC TGGGGCATCC ATTCTGTGAA 
AACTTGAGGT CGGGAGACTG GATGATCGAC TACGTGAGTG GTCGGCTTAT TTCAAGATCA 
GGAAGTATTG CTGAGGTTGG TAAATGGCTG CAGGCTATGT TCTTCTACCT GAAGCAGATC 
CCACGCTACC TCATCCCCTG CTACTTTGAC GCTATATTGA TTGGTGCATA CACCACTCTG 
CTGGATGTAG CGTGGAAACA GATGTCAAGC TTTGTTCAGA ACGGATCCAC CTTCGTGAAG 
CACCTGTCAC TGGGTTCTGT CCAGATGTGT GGAGTCGGGA AATGCCCTTG TCTGCCGCTG 
CTTTCTCCCT CGCTCCTGGA TGTGCCGTGC AGGTTAAATG AGATCACAAA GGAGAAGGAG 
CAGTGCTGTG CCTCCTTAGC TGCAGGCTTA CCTCACTTTT CTTCCGGTCT TTTTCGCTGC 
TGGGGGAGGG ACACTTTCAT TGCCCTTCGA GGGATGCTGC TGGTTACTGG ACGCTACCTG 
GAAGCCAGGA ACATCATTTT AGCGTTTGCT AGTACCCTGC GGCACGGTCT CATCCCTAAC 
CTCCTCGGCG AAGGGACATA TGCCAGGTAC AACTGCCGGG ACGCCGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATTG CAGGACAGTT CCAAACGGCC TGGACATCCT CAAATGCCCG 
GTGTCCAGGA TGTATCCAAC AGATGACTCC GCACCCTTGC CTGCTGGGAC ACTGGACCAG 
CCTTTGTTTG AAGTCATCCA GGAAGCTATG CAGAGGCACA TGCAGGGCAT CCAGTTCCGA 
GAGAGGAACG CCGGTCCACA GATCGACCGA AACATGAAGG ACGAGGGTTT CAACATAACT 
GCGGGGATTG ATGAAGAAAC AGGATTTGTT TATGGAGGAA ATCGCTTCAA CTGTGGCACG 
TGGATGGATA AAATGGGAGA GAGTGACCGA GCTAGGAACA GAGGGATCCC AGCCACTCCA 
AGAGATGGGT CTGCTGTGGA AATCGTGGGC TTGTGTAAAT CTGCTGTTCG TTGGTTGCTG 
GAATTGTCTA AGAAAAATAT CTTTCCTTAT CATGAAGTCA GAGTAAAAAG ACATGGAAAG 
GTTGTGGCAG TCTCGTACGA TGAGTGGAAC AGAAAAATAC AAAACAATTT CGAAAAGCTG 
TTTCATGTTT CCGAAGACCC TTCAGATCCT AACGAGAAGC ATCCGAACCT GGTTCACAAA 
CGCGGCATCT ACAAGGACAG CTATGGTGCG TCGAGTCCTT GGTGTGACTA TCAGCTCAGG 
CCCAACTTCA CCATAGCAAT GGTCGTGGCC CCTGAACTCT TTACTGCTGA GAAGGCTTGG 
AAGGCTTTGG AAATTGCAGA AAAAAAATTG CTTGGTCCCC TTGGCATGAA AACTTTAGAC 
CCAGATGACA TGGTTTACTG TGGAGTTTAT GATAATGCCT TAGACAATGA CAACTACAAC 
CTTGCTAGAG GCTTCAATTA CCACCAAGGA CCTGAGTGGC TGTGGCCAAT TGGGTATTTT 
CTTCGTGCAA AGTTGTATTT TTCCAAAATG ATGGGTCCAG AGACTGCTGC GAAGACTGTA 
TTTTTGGTTA AAAATGTTCT TTCTAGACAT TATGTTCATC TTGAGAGATC CCCATGGAAA 
GGCCTTCCGG AGCTCACCAA TGAGAACGGC CAGTACTGCC CTTTCAGCTG TGAGACACAG 
GCGTGGTCAA TGGCTGTTGT TCTGGAAACC CTCTATGATT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( NM_001362367.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349296.1 Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83950
Clone ID Related Accession (Same CDS sequence)	NM_001362367.1
Accession Version	NM_001362367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Mus musculus(house mouse)
Product	glycogen debranching enzyme isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC147242.4 and AK036225.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTACTGAACG AGATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TCACTGTATA TACAAATTAC CCATTGCCTG GAGAAGCATT TAATCGAGAA 
AAGTTCCGTT CCTTGGATTG GGAAAACCCA ACGGAGAGAG AAGACGATTC TGATAAATAC 
TGTAAACTTC ATCTGCAGCA GTCTGGATCT TTTCAGTACT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGAG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GCATTGGAGT TGATAACCAC 
GTGTTACCCT TGGACTGTGT TACTCTGCAG ACGTTCTTAG CAAAGTGCTT AGGACCCTTC 
GATGAGTGGG AAAGCCGGCT AAGGGTGGCA AAGGAGTCAG GTTACAACAT GATTCACTTC 
ACCCCACTGC AGACTCTTGG ACTGTCCCGG TCATGCTATT CCCTTGCCGA TCAGTTAGAG 
CTAAATCCTG ACTTCTCAAG GCCTAGTAAA AGATATACCT GGAGTGACGT TGGGCAGCTA 
GTGGAGAAGC TGAAAAGGGA GTGGAATATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCCACTA ATAGTAAGTG GATCCTTGAA CACCCGGAAT CTGCATACAA TCTTGTAAAT 
TCTCCACACT TGAAGCCTGC CTGGGTCCTA GACAGAGCAC TCTGGCATTT CTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTT TGATTGAAAA TGACCAACAC 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA ACTCTGGGAG 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCAAAA GAACATCTTA AAATTATTCA GGACCCTGAA 
TACAGGCGGC GAGGCTGCGC TGTAGATATG GACACTGCTC TGGCGACTTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAACTGGT TCCGTAAGAG ACTGGAGGAG 
TTAAACTCAG AGAAGCACCA CCTCACCAGC TGTCACCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGACTGGCA GGCCATGGCC CTAAACTAGG ACCTGTCACC 
AGGAAGTATC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGGAGAAAT GGCCTTATCC 
GCAGAGGAAG CCCTGATCCA CCTCCCAGAT AAAGCGTGCT TCCTGATGGC ACACAACGGG 
TGGGTGATGG GCGATGACCC GCTCCGGAAC TTTGCCGAGC CAGGTTCGGA TGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTTAAATTGC GCTATGGGAA TAAACCCGAG 
GACTGCCCTT ATCTGTGGGC ACACATGAAG AAATACACCG AGATAACTGC CACTCACTTC 
CAGGGAGTTC GTCTGGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGAGCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCGT ATAACAGTCA CGAAGAGGGC AGACTGGTCT ACCGGTACGG AGGGGAACCT 
GTCGGCTCCT TCGTTCAGCC CTGTTTGAGG CCGCTCATGC CTGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCGATTGTGC ACAGATCAGC CTATGATGCT 
CTTCCAAGTA CCACAGTTGT TTCCATGGCA TGCTGTGCCA GTGGAAGCAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCAGAGG AGCGATTTTA CACTAAGTGG 
AATCCTGGGG CATCACCGGC AGATACAGGG GATGTTAATG TCCATAGCGG GATTATTGCA 
GCCAGATGTG CCATCAATAG GCTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCTGTAACCA GGCACTCCCC GAGCATCCAC 
CAGTCTGTTG TGGCTGTATC GAGAACTGCT TTTAGGAATC CCAAGACTTC CTTTTACAGC 
AAAGAAGTGC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAAA GGAACACAAA ACCTTATAAG AAAGACGAGA ACTCGATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACACATC CAGCTTCATG AAAGTAAAAT TGTCAGACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAGAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTCGATC CACACGCTCA AGTTGCTGTG 
GGGATTCTTC GGAACCACCT GACCCAGTTC AGCTCTCACT TTAAGTCCGG AAGCCTTGCT 
GTTGACAACG CAGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAACCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGC 
GGTTGCTATG ACATACCAAA CTGGTCATCA CTTAAGTACG CAGGACTTCA AGGATTAATG 
TCCGTGTTGG CAGAAATAAG ACCGAAGAAT GACCTGGGGC ATCCATTCTG TGAAAACTTG 
AGGTCGGGAG ACTGGATGAT CGACTACGTG AGTGGTCGGC TTATTTCAAG ATCAGGAAGT 
ATTGCTGAGG TTGGTAAATG GCTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGC 
TACCTCATCC CCTGCTACTT TGACGCTATA TTGATTGGTG CATACACCAC TCTGCTGGAT 
GTAGCGTGGA AACAGATGTC AAGCTTTGTT CAGAACGGAT CCACCTTCGT GAAGCACCTG 
TCACTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAATGCC CTTGTCTGCC GCTGCTTTCT 
CCCTCGCTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CAAAGGAGAA GGAGCAGTGC 
TGTGCCTCCT TAGCTGCAGG CTTACCTCAC TTTTCTTCCG GTCTTTTTCG CTGCTGGGGG 
AGGGACACTT TCATTGCCCT TCGAGGGATG CTGCTGGTTA CTGGACGCTA CCTGGAAGCC 
AGGAACATCA TTTTAGCGTT TGCTAGTACC CTGCGGCACG GTCTCATCCC TAACCTCCTC 
GGCGAAGGGA CATATGCCAG GTACAACTGC CGGGACGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAGGAC AGTTCCAAAC GGCCTGGACA TCCTCAAATG CCCGGTGTCC 
AGGATGTATC CAACAGATGA CTCCGCACCC TTGCCTGCTG GGACACTGGA CCAGCCTTTG 
TTTGAAGTCA TCCAGGAAGC TATGCAGAGG CACATGCAGG GCATCCAGTT CCGAGAGAGG 
AACGCCGGTC CACAGATCGA CCGAAACATG AAGGACGAGG GTTTCAACAT AACTGCGGGG 
ATTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACGTGGATG 
GATAAAATGG GAGAGAGTGA CCGAGCTAGG AACAGAGGGA TCCCAGCCAC TCCAAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349296.1
CDS	208..4047
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLDWENPTEREDDSDKYCKLHLQQSGSFQYYFLQGNEKSGGGYIVVDPILRIGVDNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPSKRYTWSDVGQLVEKLKREWNILCITDVVYNHTATNSKWILEHPESAYNLVNSPHLKPAWVLDRALWHFSCDVADGKYREKGVPALIENDQHMNCIRKIIWEDIFPRIQLWEFFQVDVHKAVEQFRRLLSQENRRVTKSEPKEHLKIIQDPEYRRRGCAVDMDTALATFIPHDNGPAAIEECCNWFRKRLEELNSEKHHLTSCHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFGEMALSAEEALIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATHFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNPGASPADTGDVNVHSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYKKDENSINGMPNMTVELREHIQLHESKIVRQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNADPILKIPFASIASKLTLAELNQVLYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSGRLISRSGSIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEARNIILAFASTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCRTVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGIDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPR

Target ORF information:

RefSeq Version	NM_001362367.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362367.1

ORF Insert Sequence:

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTACTGAACG AGATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TCACTGTATA TACAAATTAC CCATTGCCTG GAGAAGCATT TAATCGAGAA 
AAGTTCCGTT CCTTGGATTG GGAAAACCCA ACGGAGAGAG AAGACGATTC TGATAAATAC 
TGTAAACTTC ATCTGCAGCA GTCTGGATCT TTTCAGTACT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGAG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GCATTGGAGT TGATAACCAC 
GTGTTACCCT TGGACTGTGT TACTCTGCAG ACGTTCTTAG CAAAGTGCTT AGGACCCTTC 
GATGAGTGGG AAAGCCGGCT AAGGGTGGCA AAGGAGTCAG GTTACAACAT GATTCACTTC 
ACCCCACTGC AGACTCTTGG ACTGTCCCGG TCATGCTATT CCCTTGCCGA TCAGTTAGAG 
CTAAATCCTG ACTTCTCAAG GCCTAGTAAA AGATATACCT GGAGTGACGT TGGGCAGCTA 
GTGGAGAAGC TGAAAAGGGA GTGGAATATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCCACTA ATAGTAAGTG GATCCTTGAA CACCCGGAAT CTGCATACAA TCTTGTAAAT 
TCTCCACACT TGAAGCCTGC CTGGGTCCTA GACAGAGCAC TCTGGCATTT CTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTT TGATTGAAAA TGACCAACAC 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA ACTCTGGGAG 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCAAAA GAACATCTTA AAATTATTCA GGACCCTGAA 
TACAGGCGGC GAGGCTGCGC TGTAGATATG GACACTGCTC TGGCGACTTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAACTGGT TCCGTAAGAG ACTGGAGGAG 
TTAAACTCAG AGAAGCACCA CCTCACCAGC TGTCACCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGACTGGCA GGCCATGGCC CTAAACTAGG ACCTGTCACC 
AGGAAGTATC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGGAGAAAT GGCCTTATCC 
GCAGAGGAAG CCCTGATCCA CCTCCCAGAT AAAGCGTGCT TCCTGATGGC ACACAACGGG 
TGGGTGATGG GCGATGACCC GCTCCGGAAC TTTGCCGAGC CAGGTTCGGA TGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTTAAATTGC GCTATGGGAA TAAACCCGAG 
GACTGCCCTT ATCTGTGGGC ACACATGAAG AAATACACCG AGATAACTGC CACTCACTTC 
CAGGGAGTTC GTCTGGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGAGCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCGT ATAACAGTCA CGAAGAGGGC AGACTGGTCT ACCGGTACGG AGGGGAACCT 
GTCGGCTCCT TCGTTCAGCC CTGTTTGAGG CCGCTCATGC CTGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCGATTGTGC ACAGATCAGC CTATGATGCT 
CTTCCAAGTA CCACAGTTGT TTCCATGGCA TGCTGTGCCA GTGGAAGCAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCAGAGG AGCGATTTTA CACTAAGTGG 
AATCCTGGGG CATCACCGGC AGATACAGGG GATGTTAATG TCCATAGCGG GATTATTGCA 
GCCAGATGTG CCATCAATAG GCTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCTGTAACCA GGCACTCCCC GAGCATCCAC 
CAGTCTGTTG TGGCTGTATC GAGAACTGCT TTTAGGAATC CCAAGACTTC CTTTTACAGC 
AAAGAAGTGC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAAA GGAACACAAA ACCTTATAAG AAAGACGAGA ACTCGATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACACATC CAGCTTCATG AAAGTAAAAT TGTCAGACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAGAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTCGATC CACACGCTCA AGTTGCTGTG 
GGGATTCTTC GGAACCACCT GACCCAGTTC AGCTCTCACT TTAAGTCCGG AAGCCTTGCT 
GTTGACAACG CAGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAACCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGC 
GGTTGCTATG ACATACCAAA CTGGTCATCA CTTAAGTACG CAGGACTTCA AGGATTAATG 
TCCGTGTTGG CAGAAATAAG ACCGAAGAAT GACCTGGGGC ATCCATTCTG TGAAAACTTG 
AGGTCGGGAG ACTGGATGAT CGACTACGTG AGTGGTCGGC TTATTTCAAG ATCAGGAAGT 
ATTGCTGAGG TTGGTAAATG GCTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGC 
TACCTCATCC CCTGCTACTT TGACGCTATA TTGATTGGTG CATACACCAC TCTGCTGGAT 
GTAGCGTGGA AACAGATGTC AAGCTTTGTT CAGAACGGAT CCACCTTCGT GAAGCACCTG 
TCACTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAATGCC CTTGTCTGCC GCTGCTTTCT 
CCCTCGCTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CAAAGGAGAA GGAGCAGTGC 
TGTGCCTCCT TAGCTGCAGG CTTACCTCAC TTTTCTTCCG GTCTTTTTCG CTGCTGGGGG 
AGGGACACTT TCATTGCCCT TCGAGGGATG CTGCTGGTTA CTGGACGCTA CCTGGAAGCC 
AGGAACATCA TTTTAGCGTT TGCTAGTACC CTGCGGCACG GTCTCATCCC TAACCTCCTC 
GGCGAAGGGA CATATGCCAG GTACAACTGC CGGGACGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAGGAC AGTTCCAAAC GGCCTGGACA TCCTCAAATG CCCGGTGTCC 
AGGATGTATC CAACAGATGA CTCCGCACCC TTGCCTGCTG GGACACTGGA CCAGCCTTTG 
TTTGAAGTCA TCCAGGAAGC TATGCAGAGG CACATGCAGG GCATCCAGTT CCGAGAGAGG 
AACGCCGGTC CACAGATCGA CCGAAACATG AAGGACGAGG GTTTCAACAT AACTGCGGGG 
ATTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACGTGGATG 
GATAAAATGG GAGAGAGTGA CCGAGCTAGG AACAGAGGGA TCCCAGCCAC TCCAAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( NM_001081326.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074795.1 Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02123
Clone ID Related Accession (Same CDS sequence)	NM_001081326.1 , XM_006502287.1
Accession Version	NM_001081326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Mus musculus(house mouse)
Product	glycogen debranching enzyme isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC147242.4. On Feb 6, 2007 this sequence version replaced XM_894172.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTACTGAACG AGATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TCACTGTATA TACAAATTAC CCATTGCCTG GAGAAGCATT TAATCGAGAA 
AAGTTCCGTT CCTTGGATTG GGAAAACCCA ACGGAGAGAG AAGACGATTC TGATAAATAC 
TGTAAACTTC ATCTGCAGCA GTCTGGATCT TTTCAGTACT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGAG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GCATTGGAGT TGATAACCAC 
GTGTTACCCT TGGACTGTGT TACTCTGCAG ACGTTCTTAG CAAAGTGCTT AGGACCCTTC 
GATGAGTGGG AAAGCCGGCT AAGGGTGGCA AAGGAGTCAG GTTACAACAT GATTCACTTC 
ACCCCACTGC AGACTCTTGG ACTGTCCCGG TCATGCTATT CCCTTGCCGA TCAGTTAGAG 
CTAAATCCTG ACTTCTCAAG GCCTAGTAAA AGATATACCT GGAGTGACGT TGGGCAGCTA 
GTGGAGAAGC TGAAAAGGGA GTGGAATATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCCACTA ATAGTAAGTG GATCCTTGAA CACCCGGAAT CTGCATACAA TCTTGTAAAT 
TCTCCACACT TGAAGCCTGC CTGGGTCCTA GACAGAGCAC TCTGGCATTT CTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTT TGATTGAAAA TGACCAACAC 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA ACTCTGGGAG 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCAAAA GAACATCTTA AAATTATTCA GGACCCTGAA 
TACAGGCGGC GAGGCTGCGC TGTAGATATG GACACTGCTC TGGCGACTTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAACTGGT TCCGTAAGAG ACTGGAGGAG 
TTAAACTCAG AGAAGCACCA CCTCACCAGC TGTCACCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGACTGGCA GGCCATGGCC CTAAACTAGG ACCTGTCACC 
AGGAAGTATC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGGAGAAAT GGCCTTATCC 
GCAGAGGAAG CCCTGATCCA CCTCCCAGAT AAAGCGTGCT TCCTGATGGC ACACAACGGG 
TGGGTGATGG GCGATGACCC GCTCCGGAAC TTTGCCGAGC CAGGTTCGGA TGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTTAAATTGC GCTATGGGAA TAAACCCGAG 
GACTGCCCTT ATCTGTGGGC ACACATGAAG AAATACACCG AGATAACTGC CACTCACTTC 
CAGGGAGTTC GTCTGGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGAGCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCGT ATAACAGTCA CGAAGAGGGC AGACTGGTCT ACCGGTACGG AGGGGAACCT 
GTCGGCTCCT TCGTTCAGCC CTGTTTGAGG CCGCTCATGC CTGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCGATTGTGC ACAGATCAGC CTATGATGCT 
CTTCCAAGTA CCACAGTTGT TTCCATGGCA TGCTGTGCCA GTGGAAGCAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCAGAGG AGCGATTTTA CACTAAGTGG 
AATCCTGGGG CATCACCGGC AGATACAGGG GATGTTAATG TCCATAGCGG GATTATTGCA 
GCCAGATGTG CCATCAATAG GCTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCTGTAACCA GGCACTCCCC GAGCATCCAC 
CAGTCTGTTG TGGCTGTATC GAGAACTGCT TTTAGGAATC CCAAGACTTC CTTTTACAGC 
AAAGAAGTGC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAAA GGAACACAAA ACCTTATAAG AAAGACGAGA ACTCGATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACACATC CAGCTTCATG AAAGTAAAAT TGTCAGACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAGAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTCGATC CACACGCTCA AGTTGCTGTG 
GGGATTCTTC GGAACCACCT GACCCAGTTC AGCTCTCACT TTAAGTCCGG AAGCCTTGCT 
GTTGACAACG CAGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAACCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGC 
GGTTGCTATG ACATACCAAA CTGGTCATCA CTTAAGTACG CAGGACTTCA AGGATTAATG 
TCCGTGTTGG CAGAAATAAG ACCGAAGAAT GACCTGGGGC ATCCATTCTG TGAAAACTTG 
AGGTCGGGAG ACTGGATGAT CGACTACGTG AGTGGTCGGC TTATTTCAAG ATCAGGAAGT 
ATTGCTGAGG TTGGTAAATG GCTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGC 
TACCTCATCC CCTGCTACTT TGACGCTATA TTGATTGGTG CATACACCAC TCTGCTGGAT 
GTAGCGTGGA AACAGATGTC AAGCTTTGTT CAGAACGGAT CCACCTTCGT GAAGCACCTG 
TCACTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAATGCC CTTGTCTGCC GCTGCTTTCT 
CCCTCGCTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CAAAGGAGAA GGAGCAGTGC 
TGTGCCTCCT TAGCTGCAGG CTTACCTCAC TTTTCTTCCG GTCTTTTTCG CTGCTGGGGG 
AGGGACACTT TCATTGCCCT TCGAGGGATG CTGCTGGTTA CTGGACGCTA CCTGGAAGCC 
AGGAACATCA TTTTAGCGTT TGCTAGTACC CTGCGGCACG GTCTCATCCC TAACCTCCTC 
GGCGAAGGGA CATATGCCAG GTACAACTGC CGGGACGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAGGAC AGTTCCAAAC GGCCTGGACA TCCTCAAATG CCCGGTGTCC 
AGGATGTATC CAACAGATGA CTCCGCACCC TTGCCTGCTG GGACACTGGA CCAGCCTTTG 
TTTGAAGTCA TCCAGGAAGC TATGCAGAGG CACATGCAGG GCATCCAGTT CCGAGAGAGG 
AACGCCGGTC CACAGATCGA CCGAAACATG AAGGACGAGG GTTTCAACAT AACTGCGGGG 
ATTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACGTGGATG 
GATAAAATGG GAGAGAGTGA CCGAGCTAGG AACAGAGGGA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATCGT GGGCTTGTGT AAATCTGCTG TTCGTTGGTT GCTGGAATTG 
TCTAAGAAAA ATATCTTTCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTTGTG 
GCAGTCTCGT ACGATGAGTG GAACAGAAAA ATACAAAACA ATTTCGAAAA GCTGTTTCAT 
GTTTCCGAAG ACCCTTCAGA TCCTAACGAG AAGCATCCGA ACCTGGTTCA CAAACGCGGC 
ATCTACAAGG ACAGCTATGG TGCGTCGAGT CCTTGGTGTG ACTATCAGCT CAGGCCCAAC 
TTCACCATAG CAATGGTCGT GGCCCCTGAA CTCTTTACTG CTGAGAAGGC TTGGAAGGCT 
TTGGAAATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGACCCAGAT 
GACATGGTTT ACTGTGGAGT TTATGATAAT GCCTTAGACA ATGACAACTA CAACCTTGCT 
AGAGGCTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CAATTGGGTA TTTTCTTCGT 
GCAAAGTTGT ATTTTTCCAA AATGATGGGT CCAGAGACTG CTGCGAAGAC TGTATTTTTG 
GTTAAAAATG TTCTTTCTAG ACATTATGTT CATCTTGAGA GATCCCCATG GAAAGGCCTT 
CCGGAGCTCA CCAATGAGAA CGGCCAGTAC TGCCCTTTCA GCTGTGAGAC ACAGGCGTGG 
TCAATGGCTG TTGTTCTGGA AACCCTCTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074795.1
CDS	208..4806
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLDWENPTEREDDSDKYCKLHLQQSGSFQYYFLQGNEKSGGGYIVVDPILRIGVDNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPSKRYTWSDVGQLVEKLKREWNILCITDVVYNHTATNSKWILEHPESAYNLVNSPHLKPAWVLDRALWHFSCDVADGKYREKGVPALIENDQHMNCIRKIIWEDIFPRIQLWEFFQVDVHKAVEQFRRLLSQENRRVTKSEPKEHLKIIQDPEYRRRGCAVDMDTALATFIPHDNGPAAIEECCNWFRKRLEELNSEKHHLTSCHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFGEMALSAEEALIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATHFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNPGASPADTGDVNVHSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYKKDENSINGMPNMTVELREHIQLHESKIVRQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNADPILKIPFASIASKLTLAELNQVLYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSGRLISRSGSIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEARNIILAFASTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCRTVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGIDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVKRHGKVVAVSYDEWNRKIQNNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKMMGPETAAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSMAVVLETLYDL

Target ORF information:

RefSeq Version	NM_001081326.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081326.1

ORF Insert Sequence:

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTACTGAACG AGATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TCACTGTATA TACAAATTAC CCATTGCCTG GAGAAGCATT TAATCGAGAA 
AAGTTCCGTT CCTTGGATTG GGAAAACCCA ACGGAGAGAG AAGACGATTC TGATAAATAC 
TGTAAACTTC ATCTGCAGCA GTCTGGATCT TTTCAGTACT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGAG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GCATTGGAGT TGATAACCAC 
GTGTTACCCT TGGACTGTGT TACTCTGCAG ACGTTCTTAG CAAAGTGCTT AGGACCCTTC 
GATGAGTGGG AAAGCCGGCT AAGGGTGGCA AAGGAGTCAG GTTACAACAT GATTCACTTC 
ACCCCACTGC AGACTCTTGG ACTGTCCCGG TCATGCTATT CCCTTGCCGA TCAGTTAGAG 
CTAAATCCTG ACTTCTCAAG GCCTAGTAAA AGATATACCT GGAGTGACGT TGGGCAGCTA 
GTGGAGAAGC TGAAAAGGGA GTGGAATATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCCACTA ATAGTAAGTG GATCCTTGAA CACCCGGAAT CTGCATACAA TCTTGTAAAT 
TCTCCACACT TGAAGCCTGC CTGGGTCCTA GACAGAGCAC TCTGGCATTT CTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTT TGATTGAAAA TGACCAACAC 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA ACTCTGGGAG 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCAAAA GAACATCTTA AAATTATTCA GGACCCTGAA 
TACAGGCGGC GAGGCTGCGC TGTAGATATG GACACTGCTC TGGCGACTTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAACTGGT TCCGTAAGAG ACTGGAGGAG 
TTAAACTCAG AGAAGCACCA CCTCACCAGC TGTCACCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGACTGGCA GGCCATGGCC CTAAACTAGG ACCTGTCACC 
AGGAAGTATC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGGAGAAAT GGCCTTATCC 
GCAGAGGAAG CCCTGATCCA CCTCCCAGAT AAAGCGTGCT TCCTGATGGC ACACAACGGG 
TGGGTGATGG GCGATGACCC GCTCCGGAAC TTTGCCGAGC CAGGTTCGGA TGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTTAAATTGC GCTATGGGAA TAAACCCGAG 
GACTGCCCTT ATCTGTGGGC ACACATGAAG AAATACACCG AGATAACTGC CACTCACTTC 
CAGGGAGTTC GTCTGGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGAGCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCGT ATAACAGTCA CGAAGAGGGC AGACTGGTCT ACCGGTACGG AGGGGAACCT 
GTCGGCTCCT TCGTTCAGCC CTGTTTGAGG CCGCTCATGC CTGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCGATTGTGC ACAGATCAGC CTATGATGCT 
CTTCCAAGTA CCACAGTTGT TTCCATGGCA TGCTGTGCCA GTGGAAGCAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCAGAGG AGCGATTTTA CACTAAGTGG 
AATCCTGGGG CATCACCGGC AGATACAGGG GATGTTAATG TCCATAGCGG GATTATTGCA 
GCCAGATGTG CCATCAATAG GCTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCTGTAACCA GGCACTCCCC GAGCATCCAC 
CAGTCTGTTG TGGCTGTATC GAGAACTGCT TTTAGGAATC CCAAGACTTC CTTTTACAGC 
AAAGAAGTGC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAAA GGAACACAAA ACCTTATAAG AAAGACGAGA ACTCGATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACACATC CAGCTTCATG AAAGTAAAAT TGTCAGACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAGAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTCGATC CACACGCTCA AGTTGCTGTG 
GGGATTCTTC GGAACCACCT GACCCAGTTC AGCTCTCACT TTAAGTCCGG AAGCCTTGCT 
GTTGACAACG CAGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAACCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGC 
GGTTGCTATG ACATACCAAA CTGGTCATCA CTTAAGTACG CAGGACTTCA AGGATTAATG 
TCCGTGTTGG CAGAAATAAG ACCGAAGAAT GACCTGGGGC ATCCATTCTG TGAAAACTTG 
AGGTCGGGAG ACTGGATGAT CGACTACGTG AGTGGTCGGC TTATTTCAAG ATCAGGAAGT 
ATTGCTGAGG TTGGTAAATG GCTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGC 
TACCTCATCC CCTGCTACTT TGACGCTATA TTGATTGGTG CATACACCAC TCTGCTGGAT 
GTAGCGTGGA AACAGATGTC AAGCTTTGTT CAGAACGGAT CCACCTTCGT GAAGCACCTG 
TCACTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAATGCC CTTGTCTGCC GCTGCTTTCT 
CCCTCGCTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CAAAGGAGAA GGAGCAGTGC 
TGTGCCTCCT TAGCTGCAGG CTTACCTCAC TTTTCTTCCG GTCTTTTTCG CTGCTGGGGG 
AGGGACACTT TCATTGCCCT TCGAGGGATG CTGCTGGTTA CTGGACGCTA CCTGGAAGCC 
AGGAACATCA TTTTAGCGTT TGCTAGTACC CTGCGGCACG GTCTCATCCC TAACCTCCTC 
GGCGAAGGGA CATATGCCAG GTACAACTGC CGGGACGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAGGAC AGTTCCAAAC GGCCTGGACA TCCTCAAATG CCCGGTGTCC 
AGGATGTATC CAACAGATGA CTCCGCACCC TTGCCTGCTG GGACACTGGA CCAGCCTTTG 
TTTGAAGTCA TCCAGGAAGC TATGCAGAGG CACATGCAGG GCATCCAGTT CCGAGAGAGG 
AACGCCGGTC CACAGATCGA CCGAAACATG AAGGACGAGG GTTTCAACAT AACTGCGGGG 
ATTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACGTGGATG 
GATAAAATGG GAGAGAGTGA CCGAGCTAGG AACAGAGGGA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATCGT GGGCTTGTGT AAATCTGCTG TTCGTTGGTT GCTGGAATTG 
TCTAAGAAAA ATATCTTTCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTTGTG 
GCAGTCTCGT ACGATGAGTG GAACAGAAAA ATACAAAACA ATTTCGAAAA GCTGTTTCAT 
GTTTCCGAAG ACCCTTCAGA TCCTAACGAG AAGCATCCGA ACCTGGTTCA CAAACGCGGC 
ATCTACAAGG ACAGCTATGG TGCGTCGAGT CCTTGGTGTG ACTATCAGCT CAGGCCCAAC 
TTCACCATAG CAATGGTCGT GGCCCCTGAA CTCTTTACTG CTGAGAAGGC TTGGAAGGCT 
TTGGAAATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGACCCAGAT 
GACATGGTTT ACTGTGGAGT TTATGATAAT GCCTTAGACA ATGACAACTA CAACCTTGCT 
AGAGGCTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CAATTGGGTA TTTTCTTCGT 
GCAAAGTTGT ATTTTTCCAA AATGATGGGT CCAGAGACTG CTGCGAAGAC TGTATTTTTG 
GTTAAAAATG TTCTTTCTAG ACATTATGTT CATCTTGAGA GATCCCCATG GAAAGGCCTT 
CCGGAGCTCA CCAATGAGAA CGGCCAGTAC TGCCCTTTCA GCTGTGAGAC ACAGGCGTGG 
TCAATGGCTG TTGTTCTGGA AACCCTCTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( XM_006502287.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502350.1 Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.

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CloneID	OMu02123
Clone ID Related Accession (Same CDS sequence)	NM_001081326.1 , XM_006502287.1
Accession Version	XM_006502287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTACTGAACG AGATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TCACTGTATA TACAAATTAC CCATTGCCTG GAGAAGCATT TAATCGAGAA 
AAGTTCCGTT CCTTGGATTG GGAAAACCCA ACGGAGAGAG AAGACGATTC TGATAAATAC 
TGTAAACTTC ATCTGCAGCA GTCTGGATCT TTTCAGTACT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGAG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GCATTGGAGT TGATAACCAC 
GTGTTACCCT TGGACTGTGT TACTCTGCAG ACGTTCTTAG CAAAGTGCTT AGGACCCTTC 
GATGAGTGGG AAAGCCGGCT AAGGGTGGCA AAGGAGTCAG GTTACAACAT GATTCACTTC 
ACCCCACTGC AGACTCTTGG ACTGTCCCGG TCATGCTATT CCCTTGCCGA TCAGTTAGAG 
CTAAATCCTG ACTTCTCAAG GCCTAGTAAA AGATATACCT GGAGTGACGT TGGGCAGCTA 
GTGGAGAAGC TGAAAAGGGA GTGGAATATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCCACTA ATAGTAAGTG GATCCTTGAA CACCCGGAAT CTGCATACAA TCTTGTAAAT 
TCTCCACACT TGAAGCCTGC CTGGGTCCTA GACAGAGCAC TCTGGCATTT CTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTT TGATTGAAAA TGACCAACAC 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA ACTCTGGGAG 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCAAAA GAACATCTTA AAATTATTCA GGACCCTGAA 
TACAGGCGGC GAGGCTGCGC TGTAGATATG GACACTGCTC TGGCGACTTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAACTGGT TCCGTAAGAG ACTGGAGGAG 
TTAAACTCAG AGAAGCACCA CCTCACCAGC TGTCACCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGACTGGCA GGCCATGGCC CTAAACTAGG ACCTGTCACC 
AGGAAGTATC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGGAGAAAT GGCCTTATCC 
GCAGAGGAAG CCCTGATCCA CCTCCCAGAT AAAGCGTGCT TCCTGATGGC ACACAACGGG 
TGGGTGATGG GCGATGACCC GCTCCGGAAC TTTGCCGAGC CAGGTTCGGA TGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTTAAATTGC GCTATGGGAA TAAACCCGAG 
GACTGCCCTT ATCTGTGGGC ACACATGAAG AAATACACCG AGATAACTGC CACTCACTTC 
CAGGGAGTTC GTCTGGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGAGCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCGT ATAACAGTCA CGAAGAGGGC AGACTGGTCT ACCGGTACGG AGGGGAACCT 
GTCGGCTCCT TCGTTCAGCC CTGTTTGAGG CCGCTCATGC CTGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCGATTGTGC ACAGATCAGC CTATGATGCT 
CTTCCAAGTA CCACAGTTGT TTCCATGGCA TGCTGTGCCA GTGGAAGCAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCAGAGG AGCGATTTTA CACTAAGTGG 
AATCCTGGGG CATCACCGGC AGATACAGGG GATGTTAATG TCCATAGCGG GATTATTGCA 
GCCAGATGTG CCATCAATAG GCTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCTGTAACCA GGCACTCCCC GAGCATCCAC 
CAGTCTGTTG TGGCTGTATC GAGAACTGCT TTTAGGAATC CCAAGACTTC CTTTTACAGC 
AAAGAAGTGC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAAA GGAACACAAA ACCTTATAAG AAAGACGAGA ACTCGATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACACATC CAGCTTCATG AAAGTAAAAT TGTCAGACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAGAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTCGATC CACACGCTCA AGTTGCTGTG 
GGGATTCTTC GGAACCACCT GACCCAGTTC AGCTCTCACT TTAAGTCCGG AAGCCTTGCT 
GTTGACAACG CAGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAACCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGC 
GGTTGCTATG ACATACCAAA CTGGTCATCA CTTAAGTACG CAGGACTTCA AGGATTAATG 
TCCGTGTTGG CAGAAATAAG ACCGAAGAAT GACCTGGGGC ATCCATTCTG TGAAAACTTG 
AGGTCGGGAG ACTGGATGAT CGACTACGTG AGTGGTCGGC TTATTTCAAG ATCAGGAAGT 
ATTGCTGAGG TTGGTAAATG GCTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGC 
TACCTCATCC CCTGCTACTT TGACGCTATA TTGATTGGTG CATACACCAC TCTGCTGGAT 
GTAGCGTGGA AACAGATGTC AAGCTTTGTT CAGAACGGAT CCACCTTCGT GAAGCACCTG 
TCACTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAATGCC CTTGTCTGCC GCTGCTTTCT 
CCCTCGCTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CAAAGGAGAA GGAGCAGTGC 
TGTGCCTCCT TAGCTGCAGG CTTACCTCAC TTTTCTTCCG GTCTTTTTCG CTGCTGGGGG 
AGGGACACTT TCATTGCCCT TCGAGGGATG CTGCTGGTTA CTGGACGCTA CCTGGAAGCC 
AGGAACATCA TTTTAGCGTT TGCTAGTACC CTGCGGCACG GTCTCATCCC TAACCTCCTC 
GGCGAAGGGA CATATGCCAG GTACAACTGC CGGGACGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAGGAC AGTTCCAAAC GGCCTGGACA TCCTCAAATG CCCGGTGTCC 
AGGATGTATC CAACAGATGA CTCCGCACCC TTGCCTGCTG GGACACTGGA CCAGCCTTTG 
TTTGAAGTCA TCCAGGAAGC TATGCAGAGG CACATGCAGG GCATCCAGTT CCGAGAGAGG 
AACGCCGGTC CACAGATCGA CCGAAACATG AAGGACGAGG GTTTCAACAT AACTGCGGGG 
ATTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACGTGGATG 
GATAAAATGG GAGAGAGTGA CCGAGCTAGG AACAGAGGGA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATCGT GGGCTTGTGT AAATCTGCTG TTCGTTGGTT GCTGGAATTG 
TCTAAGAAAA ATATCTTTCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTTGTG 
GCAGTCTCGT ACGATGAGTG GAACAGAAAA ATACAAAACA ATTTCGAAAA GCTGTTTCAT 
GTTTCCGAAG ACCCTTCAGA TCCTAACGAG AAGCATCCGA ACCTGGTTCA CAAACGCGGC 
ATCTACAAGG ACAGCTATGG TGCGTCGAGT CCTTGGTGTG ACTATCAGCT CAGGCCCAAC 
TTCACCATAG CAATGGTCGT GGCCCCTGAA CTCTTTACTG CTGAGAAGGC TTGGAAGGCT 
TTGGAAATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGACCCAGAT 
GACATGGTTT ACTGTGGAGT TTATGATAAT GCCTTAGACA ATGACAACTA CAACCTTGCT 
AGAGGCTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CAATTGGGTA TTTTCTTCGT 
GCAAAGTTGT ATTTTTCCAA AATGATGGGT CCAGAGACTG CTGCGAAGAC TGTATTTTTG 
GTTAAAAATG TTCTTTCTAG ACATTATGTT CATCTTGAGA GATCCCCATG GAAAGGCCTT 
CCGGAGCTCA CCAATGAGAA CGGCCAGTAC TGCCCTTTCA GCTGTGAGAC ACAGGCGTGG 
TCAATGGCTG TTGTTCTGGA AACCCTCTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502350.1
CDS	543..5141
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLDWENPTEREDDSDKYCKLHLQQSGSFQYYFLQGNEKSGGGYIVVDPILRIGVDNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPSKRYTWSDVGQLVEKLKREWNILCITDVVYNHTATNSKWILEHPESAYNLVNSPHLKPAWVLDRALWHFSCDVADGKYREKGVPALIENDQHMNCIRKIIWEDIFPRIQLWEFFQVDVHKAVEQFRRLLSQENRRVTKSEPKEHLKIIQDPEYRRRGCAVDMDTALATFIPHDNGPAAIEECCNWFRKRLEELNSEKHHLTSCHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFGEMALSAEEALIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATHFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNPGASPADTGDVNVHSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYKKDENSINGMPNMTVELREHIQLHESKIVRQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNADPILKIPFASIASKLTLAELNQVLYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSGRLISRSGSIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEARNIILAFASTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCRTVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGIDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVKRHGKVVAVSYDEWNRKIQNNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKMMGPETAAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSMAVVLETLYDL

Target ORF information:

RefSeq Version	XM_006502287.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502287.1

ORF Insert Sequence:

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTACTGAACG AGATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TCACTGTATA TACAAATTAC CCATTGCCTG GAGAAGCATT TAATCGAGAA 
AAGTTCCGTT CCTTGGATTG GGAAAACCCA ACGGAGAGAG AAGACGATTC TGATAAATAC 
TGTAAACTTC ATCTGCAGCA GTCTGGATCT TTTCAGTACT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGAG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GCATTGGAGT TGATAACCAC 
GTGTTACCCT TGGACTGTGT TACTCTGCAG ACGTTCTTAG CAAAGTGCTT AGGACCCTTC 
GATGAGTGGG AAAGCCGGCT AAGGGTGGCA AAGGAGTCAG GTTACAACAT GATTCACTTC 
ACCCCACTGC AGACTCTTGG ACTGTCCCGG TCATGCTATT CCCTTGCCGA TCAGTTAGAG 
CTAAATCCTG ACTTCTCAAG GCCTAGTAAA AGATATACCT GGAGTGACGT TGGGCAGCTA 
GTGGAGAAGC TGAAAAGGGA GTGGAATATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCCACTA ATAGTAAGTG GATCCTTGAA CACCCGGAAT CTGCATACAA TCTTGTAAAT 
TCTCCACACT TGAAGCCTGC CTGGGTCCTA GACAGAGCAC TCTGGCATTT CTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTT TGATTGAAAA TGACCAACAC 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA ACTCTGGGAG 
TTTTTCCAAG TAGATGTTCA CAAAGCAGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCAAAA GAACATCTTA AAATTATTCA GGACCCTGAA 
TACAGGCGGC GAGGCTGCGC TGTAGATATG GACACTGCTC TGGCGACTTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAACTGGT TCCGTAAGAG ACTGGAGGAG 
TTAAACTCAG AGAAGCACCA CCTCACCAGC TGTCACCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGACTGGCA GGCCATGGCC CTAAACTAGG ACCTGTCACC 
AGGAAGTATC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGGAGAAAT GGCCTTATCC 
GCAGAGGAAG CCCTGATCCA CCTCCCAGAT AAAGCGTGCT TCCTGATGGC ACACAACGGG 
TGGGTGATGG GCGATGACCC GCTCCGGAAC TTTGCCGAGC CAGGTTCGGA TGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTTAAATTGC GCTATGGGAA TAAACCCGAG 
GACTGCCCTT ATCTGTGGGC ACACATGAAG AAATACACCG AGATAACTGC CACTCACTTC 
CAGGGAGTTC GTCTGGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGAGCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCGT ATAACAGTCA CGAAGAGGGC AGACTGGTCT ACCGGTACGG AGGGGAACCT 
GTCGGCTCCT TCGTTCAGCC CTGTTTGAGG CCGCTCATGC CTGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCGATTGTGC ACAGATCAGC CTATGATGCT 
CTTCCAAGTA CCACAGTTGT TTCCATGGCA TGCTGTGCCA GTGGAAGCAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCAGAGG AGCGATTTTA CACTAAGTGG 
AATCCTGGGG CATCACCGGC AGATACAGGG GATGTTAATG TCCATAGCGG GATTATTGCA 
GCCAGATGTG CCATCAATAG GCTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCTGTAACCA GGCACTCCCC GAGCATCCAC 
CAGTCTGTTG TGGCTGTATC GAGAACTGCT TTTAGGAATC CCAAGACTTC CTTTTACAGC 
AAAGAAGTGC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAAA GGAACACAAA ACCTTATAAG AAAGACGAGA ACTCGATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACACATC CAGCTTCATG AAAGTAAAAT TGTCAGACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAGAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTCGATC CACACGCTCA AGTTGCTGTG 
GGGATTCTTC GGAACCACCT GACCCAGTTC AGCTCTCACT TTAAGTCCGG AAGCCTTGCT 
GTTGACAACG CAGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAACCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGC 
GGTTGCTATG ACATACCAAA CTGGTCATCA CTTAAGTACG CAGGACTTCA AGGATTAATG 
TCCGTGTTGG CAGAAATAAG ACCGAAGAAT GACCTGGGGC ATCCATTCTG TGAAAACTTG 
AGGTCGGGAG ACTGGATGAT CGACTACGTG AGTGGTCGGC TTATTTCAAG ATCAGGAAGT 
ATTGCTGAGG TTGGTAAATG GCTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGC 
TACCTCATCC CCTGCTACTT TGACGCTATA TTGATTGGTG CATACACCAC TCTGCTGGAT 
GTAGCGTGGA AACAGATGTC AAGCTTTGTT CAGAACGGAT CCACCTTCGT GAAGCACCTG 
TCACTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAATGCC CTTGTCTGCC GCTGCTTTCT 
CCCTCGCTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CAAAGGAGAA GGAGCAGTGC 
TGTGCCTCCT TAGCTGCAGG CTTACCTCAC TTTTCTTCCG GTCTTTTTCG CTGCTGGGGG 
AGGGACACTT TCATTGCCCT TCGAGGGATG CTGCTGGTTA CTGGACGCTA CCTGGAAGCC 
AGGAACATCA TTTTAGCGTT TGCTAGTACC CTGCGGCACG GTCTCATCCC TAACCTCCTC 
GGCGAAGGGA CATATGCCAG GTACAACTGC CGGGACGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAGGAC AGTTCCAAAC GGCCTGGACA TCCTCAAATG CCCGGTGTCC 
AGGATGTATC CAACAGATGA CTCCGCACCC TTGCCTGCTG GGACACTGGA CCAGCCTTTG 
TTTGAAGTCA TCCAGGAAGC TATGCAGAGG CACATGCAGG GCATCCAGTT CCGAGAGAGG 
AACGCCGGTC CACAGATCGA CCGAAACATG AAGGACGAGG GTTTCAACAT AACTGCGGGG 
ATTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACGTGGATG 
GATAAAATGG GAGAGAGTGA CCGAGCTAGG AACAGAGGGA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATCGT GGGCTTGTGT AAATCTGCTG TTCGTTGGTT GCTGGAATTG 
TCTAAGAAAA ATATCTTTCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTTGTG 
GCAGTCTCGT ACGATGAGTG GAACAGAAAA ATACAAAACA ATTTCGAAAA GCTGTTTCAT 
GTTTCCGAAG ACCCTTCAGA TCCTAACGAG AAGCATCCGA ACCTGGTTCA CAAACGCGGC 
ATCTACAAGG ACAGCTATGG TGCGTCGAGT CCTTGGTGTG ACTATCAGCT CAGGCCCAAC 
TTCACCATAG CAATGGTCGT GGCCCCTGAA CTCTTTACTG CTGAGAAGGC TTGGAAGGCT 
TTGGAAATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGACCCAGAT 
GACATGGTTT ACTGTGGAGT TTATGATAAT GCCTTAGACA ATGACAACTA CAACCTTGCT 
AGAGGCTTCA ATTACCACCA AGGACCTGAG TGGCTGTGGC CAATTGGGTA TTTTCTTCGT 
GCAAAGTTGT ATTTTTCCAA AATGATGGGT CCAGAGACTG CTGCGAAGAC TGTATTTTTG 
GTTAAAAATG TTCTTTCTAG ACATTATGTT CATCTTGAGA GATCCCCATG GAAAGGCCTT 
CCGGAGCTCA CCAATGAGAA CGGCCAGTAC TGCCCTTTCA GCTGTGAGAC ACAGGCGTGG 
TCAATGGCTG TTGTTCTGGA AACCCTCTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sporadic Hirschsprung Disease: Mutational Spectrum and Novel Candidate Genes Revealed by Next-generation Sequencing.
Scientific reports7(1)14796(2017 Nov)
Zhang Z,Li Q,Diao M,Liu N,Cheng W,Xiao P,Zou J,Su L,Yu K,Wu J,Li L,Jiang Q

?H NMR metabolic profiling of plasma reveals additional phenotypes in knockout mouse models.
Journal of proteome research14(5)2036-45(2015 May)
Probert F,Rice P,Scudamore CL,Wells S,Williams R,Hough TA,Cox IJ

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Agl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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