AGL cDNA ORF clone, Canis lupus familiaris(dog)

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe33753 NM_001048096.1
Latest version!
Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe07839 XM_005621310.3
Latest version!
Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe07839 XM_005621312.3
Latest version!
Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe07839 XM_005621311.3
Latest version!
Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OCe07839 XM_005621311.2 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe07839 XM_005621312.2 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe07839 XM_005621310.2 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe33753
    Clone ID Related Accession (Same CDS sequence) NM_001048096.1
    Accession Version NM_001048096.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Canis lupus familiaris(dog)
    Product glycogen debranching enzyme
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ307574.1. On Sep 15, 2006 this sequence version replaced XM_537057.2. ##Evidence-Data-START## Transcript exon combination :: DQ307574.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

    1
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT GTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTYC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001041561.1
    CDS171..4772
    Translation

    Target ORF information:

    RefSeq Version NM_001048096.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001048096.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT GTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTYC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07839
    Clone ID Related Accession (Same CDS sequence) XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
    Accession Version XM_005621311.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product glycogen debranching enzyme isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621311.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621368.1
    CDS348..4949
    Translation

    Target ORF information:

    RefSeq Version XM_005621311.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621311.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07839
    Clone ID Related Accession (Same CDS sequence) XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
    Accession Version XM_005621311.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621311.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621368.1
    CDS351..4952
    Translation

    Target ORF information:

    RefSeq Version XM_005621311.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621311.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07839
    Clone ID Related Accession (Same CDS sequence) XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
    Accession Version XM_005621312.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product glycogen debranching enzyme isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621369.1
    CDS228..4829
    Translation

    Target ORF information:

    RefSeq Version XM_005621312.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621312.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07839
    Clone ID Related Accession (Same CDS sequence) XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
    Accession Version XM_005621310.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product glycogen debranching enzyme isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621367.1
    CDS175..4776
    Translation

    Target ORF information:

    RefSeq Version XM_005621310.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621310.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07839
    Clone ID Related Accession (Same CDS sequence) XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
    Accession Version XM_005621312.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621312.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621369.1
    CDS113..4714
    Translation

    Target ORF information:

    RefSeq Version XM_005621312.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621312.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe07839
    Clone ID Related Accession (Same CDS sequence) XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
    Accession Version XM_005621310.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621310.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621367.1
    CDS175..4776
    Translation

    Target ORF information:

    RefSeq Version XM_005621310.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621310.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG
    GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA
    AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
    TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG
    AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC
    GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT
    GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT
    ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA
    TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA
    GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC
    GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA
    ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA
    TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
    AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT
    GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA
    CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG
    GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC
    CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC
    ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC
    TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT
    GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC
    CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA
    GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT
    TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG
    TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA
    AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG
    GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA
    CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT
    CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT
    GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC
    TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG
    TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT
    GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG
    GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC
    CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC
    AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT
    AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG
    CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG
    CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC
    TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT
    GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT
    GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA
    ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT
    GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA
    ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT
    TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA
    ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA
    CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG
    GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC
    CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT
    TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA
    TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG
    GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG
    GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC
    CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG
    TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT
    TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA
    CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG
    AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA
    GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG
    ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA
    GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA
    TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT
    GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT
    CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT
    GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT
    AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA
    GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA
    GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT
    GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT
    CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT
    TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA
    CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC
    TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.