ORF » Species Summary » Homo sapiens » AGL cDNA ORF clone

AGL cDNA ORF clone, Homo sapiens(human)

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16218 NM_000643.2
Latest version!		 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$274.50-$384.30
$549.00
OHu16218 NM_000642.3
Latest version!		 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$274.50-$384.30
$549.00
OHu16218 NM_000028.2
Latest version!		 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$274.50-$384.30
$549.00
OHu16218 NM_000644.2
Latest version!		 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$274.50-$384.30
$549.00
OHu16218 XM_005270557.2
Latest version!		 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$274.50-$384.30
$549.00
OHu83253 XM_017000501.2
Latest version!		 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu16190 NM_000646.2
Latest version!		 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
View Old Accession Versions >>
OHu16218 NM_000642.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$274.50-$384.30
$549.00
OHu16218 XM_005270557.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$274.50-$384.30
$549.00
OHu83253 XM_017000501.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    AGL ( NM_000643.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000634.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu16218
    Clone ID Related Accession (Same CDS sequence) NM_000643.2 , XM_005270557.2 , NM_000644.2 , NM_000642.3 , NM_000642.2 , XM_005270557.1 , NM_000028.2
    Accession Version NM_000643.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-22
    Organism Homo sapiens(human)
    Product glycogen debranching enzyme isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84009.1 and AC096949.2. On Oct 27, 2006 this sequence version replaced NM_000643.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84009.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000634.2
    CDS199..4797
    Translation

    Target ORF information:

    RefSeq Version NM_000643.2
    Organism Homo sapiens(human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000643.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    AGL ( NM_000644.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000635.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu16218
    Clone ID Related Accession (Same CDS sequence) NM_000643.2 , XM_005270557.2 , NM_000644.2 , NM_000642.3 , NM_000642.2 , XM_005270557.1 , NM_000028.2
    Accession Version NM_000644.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Homo sapiens(human)
    Product glycogen debranching enzyme isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84008.1 and AC096949.2. On Oct 27, 2006 this sequence version replaced NM_000644.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267751, SAMN03267756 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000635.2
    CDS139..4737
    Translation

    Target ORF information:

    RefSeq Version NM_000644.2
    Organism Homo sapiens(human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000644.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    AGL ( NM_000642.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000633.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu16218
    Clone ID Related Accession (Same CDS sequence) NM_000643.2 , XM_005270557.2 , NM_000644.2 , NM_000642.3 , NM_000642.2 , XM_005270557.1 , NM_000028.2
    Accession Version NM_000642.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-26
    Organism Homo sapiens(human)
    Product glycogen debranching enzyme isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84007.1, BC078663.1, AC096949.2 and AK130308.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000642.2. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84007.1, SRR1660809.258405.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361915.8/ ENSP00000355106.3 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000633.2
    CDS124..4722
    Translation

    Target ORF information:

    RefSeq Version NM_000642.3
    Organism Homo sapiens(human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000642.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    AGL ( NM_000642.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000633.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu16218
    Clone ID Related Accession (Same CDS sequence) NM_000643.2 , XM_005270557.2 , NM_000644.2 , NM_000642.3 , NM_000642.2 , XM_005270557.1 , NM_000028.2
    Accession Version NM_000642.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product glycogen debranching enzyme isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84007.1, BC078663.1, AC096949.2, AK130308.1 and C00487.1. This sequence is a reference standard in the RefSeqGene project. On Oct 27, 2006 this sequence version replaced NM_000642.1. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84007.1, SRR1660809.258405.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000633.2
    CDS401..4999
    Translation

    Target ORF information:

    RefSeq Version NM_000642.2
    Organism Homo sapiens(human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000642.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    AGL ( NM_000028.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000019.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID OHu16218
    Clone ID Related Accession (Same CDS sequence) NM_000643.2 , XM_005270557.2 , NM_000644.2 , NM_000642.3 , NM_000642.2 , XM_005270557.1 , NM_000028.2
    Accession Version NM_000028.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Homo sapiens(human)
    Product glycogen debranching enzyme isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84010.1 and AC096949.2. On Oct 27, 2006 this sequence version replaced NM_000028.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (1). Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000019.2
    CDS479..5077
    Translation

    Target ORF information:

    RefSeq Version NM_000028.2
    Organism Homo sapiens(human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000028.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    AGL ( XM_005270557.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270614.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu16218
    Clone ID Related Accession (Same CDS sequence) NM_000643.2 , XM_005270557.2 , NM_000644.2 , NM_000642.3 , NM_000642.2 , XM_005270557.1 , NM_000028.2
    Accession Version XM_005270557.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005270614.1
    CDS94..4692
    Translation

    Target ORF information:

    RefSeq Version XM_005270557.2
    Organism Homo sapiens(human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005270557.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    AGL ( XM_005270557.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270614.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu16218
    Clone ID Related Accession (Same CDS sequence) NM_000643.2 , XM_005270557.2 , NM_000644.2 , NM_000642.3 , NM_000642.2 , XM_005270557.1 , NM_000028.2
    Accession Version XM_005270557.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product glycogen debranching enzyme isoform X1
    Comment 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005270614.1
    Translation

    Target ORF information:

    RefSeq Version XM_005270557.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005270557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
    ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
    GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
    AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
    TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
    GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
    GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
    ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
    TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
    GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
    ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
    TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
    GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
    ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
    TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
    AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
    TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
    GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
    TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
    TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
    AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
    ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
    TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
    AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
    GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
    CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
    GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
    GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    AGL ( XM_017000501.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855990.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu83253
    Clone ID Related Accession (Same CDS sequence) XM_017000501.1 , XM_017000501.2
    Accession Version XM_017000501.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product glycogen debranching enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855990.1
    CDS68..2926
    Translation

    Target ORF information:

    RefSeq Version XM_017000501.1
    Organism Homo sapiens(human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017000501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    AGL ( XM_017000501.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855990.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu83253
    Clone ID Related Accession (Same CDS sequence) XM_017000501.1 , XM_017000501.2
    Accession Version XM_017000501.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product glycogen debranching enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855990.1
    CDS69..2927
    Translation

    Target ORF information:

    RefSeq Version XM_017000501.2
    Organism Homo sapiens(human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017000501.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
    GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
    TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
    CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
    GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
    AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
    GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
    TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
    CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
    AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
    ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
    GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
    GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
    TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
    GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
    GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
    TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
    GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
    TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
    AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
    ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
    TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
    ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
    TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
    CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
    TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
    AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
    AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
    GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
    ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
    AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
    TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
    AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
    GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
    GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
    GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
    TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
    AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
    GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
    ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
    TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
    TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
    GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
    AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
    GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
    GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
    CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
    TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    AGL ( NM_000646.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000637.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 6, mRNA.
    Price & Availability↑
    CloneID OHu16190
    Clone ID Related Accession (Same CDS sequence) NM_000646.2
    Accession Version NM_000646.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Homo sapiens(human)
    Product glycogen debranching enzyme isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84011.1 and AC096949.2. On or before Aug 31, 2013 this sequence version replaced XM_005270558.1, NM_000646.1. Transcript Variant: This variant (6) differs in the 5' UTR and coding region, compared to variant 4. The resulting protein (isoform 3) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84011.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820701, SAMN04284274 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCCCCAA TTCTGAGCAT TAATTTATTT ATTGGGTATG AGCTACAGTT CCGATTAGGC 
    CCAACTTTAC AGGGAAAAGC AGTTACCGTG TATACAAATT ACCCATTTCC TGGAGAAACA 
    TTTAATAGAG AAAAATTCCG TTCTCTGGAT TGGGAAAATC CAACAGAAAG AGAAGATGAT 
    TCTGATAAAT ACTGTAAACT TAATCTGCAA CAATCTGGTT CATTTCAGTA TTATTTCCTT 
    CAAGGAAATG AGAAAAGTGG TGGAGGTTAC ATAGTTGTGG ACCCCATTTT ACGTGTTGGT 
    GCTGATAATC ATGTGCTACC CTTGGACTGT GTTACTCTTC AGACATTTTT AGCTAAGTGT 
    TTGGGACCTT TTGATGAATG GGAAAGCAGA CTTAGGGTTG CAAAAGAATC AGGCTACAAC 
    ATGATTCATT TTACCCCATT GCAGACTCTT GGACTATCTA GGTCATGCTA CTCCCTTGCC 
    AATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTAATA GAAAGTATAC CTGGAATGAT 
    GTTGGACAGC TAGTGGAAAA ATTAAAAAAG GAATGGAATG TTATTTGTAT TACTGATGTT 
    GTCTACAATC ATACTGCTGC TAATAGTAAA TGGATCCAGG AACATCCAGA ATGTGCCTAT 
    AATCTTGTGA ATTCTCCACA CTTAAAACCT GCCTGGGTCT TAGACAGAGC ACTTTGGCGT 
    TTCTCCTGTG ATGTTGCAGA AGGGAAATAC AAAGAAAAGG GAATACCTGC TTTGATTGAA 
    AATGATCACC ATATGAATTC CATCCGAAAA ATAATTTGGG AGGATATTTT TCCAAAGCTT 
    AAACTCTGGG AATTTTTCCA AGTAGATGTC AACAAAGCGG TTGAGCAATT TAGAAGACTT 
    CTTACACAAG AAAATAGGCG AGTAACCAAG TCTGATCCAA ACCAACACCT TACGATTATT 
    CAAGATCCTG AATACAGACG GTTTGGCTGT ACTGTAGATA TGAACATTGC ACTAACGACT 
    TTCATACCAC ATGACAAGGG GCCAGCAGCA ATTGAAGAAT GCTGTAATTG GTTTCATAAA 
    AGAATGGAGG AATTAAATTC AGAGAAGCAT CGACTCATTA ACTATCATCA GGAACAGGCA 
    GTTAATTGCC TTTTGGGAAA TGTGTTTTAT GAACGACTGG CTGGCCATGG TCCAAAACTA 
    GGACCTGTCA CTAGAAAGCA TCCTTTAGTT ACCAGGTATT TTACTTTCCC ATTTGAAGAG 
    ATAGACTTCT CCATGGAAGA ATCTATGATT CATCTGCCAA ATAAAGCTTG TTTTCTGATG 
    GCACACAATG GATGGGTAAT GGGAGATGAT CCTCTTCGAA ACTTTGCTGA ACCGGGTTCA 
    GAAGTTTACC TAAGGAGAGA ACTTATTTGC TGGGGAGACA GTGTTAAATT ACGCTATGGG 
    AATAAACCAG AGGACTGTCC TTATCTCTGG GCACACATGA AAAAATACAC TGAAATAACT 
    GCAACTTATT TCCAGGGAGT ACGTCTTGAT AACTGCCACT CAACACCTCT TCACGTAGCT 
    GAGTACATGT TGGATGCTGC TAGGAATTTG CAACCCAATT TATATGTAGT AGCTGAACTG 
    TTCACAGGAA GTGAAGATCT GGACAATGTC TTTGTTACTA GACTGGGCAT TAGTTCCTTA 
    ATAAGAGAGG CAATGAGTGC ATATAATAGT CATGAAGAGG GCAGATTAGT TTACCGATAT 
    GGAGGAGAAC CTGTTGGATC CTTTGTTCAG CCCTGTTTGA GGCCTTTAAT GCCAGCTATT 
    GCACATGCCC TGTTTATGGA TATTACGCAT GATAATGAGT GTCCTATTGT GCATAGATCA 
    GCGTATGATG CTCTTCCAAG TACTACAATT GTTTCTATGG CATGTTGTGC TAGTGGAAGT 
    ACAAGAGGCT ATGATGAATT AGTGCCTCAT CAGATTTCAG TGGTTTCTGA AGAACGGTTT 
    TACACTAAGT GGAATCCTGA AGCATTGCCT TCAAACACAG GTGAAGTTAA TTTCCAAAGC 
    GGCATTATTG CAGCCAGGTG TGCTATCAGT AAACTTCATC AGGAGCTTGG AGCCAAGGGT 
    TTTATTCAGG TGTATGTGGA TCAAGTTGAT GAAGACATAG TGGCAGTAAC AAGACACTCA 
    CCTAGCATCC ATCAGTCTGT TGTGGCTGTA TCTAGAACTG CTTTCAGGAA TCCCAAGACT 
    TCATTTTACA GCAAGGAAGT GCCTCAAATG TGCATCCCTG GCAAAATTGA AGAAGTAGTT 
    CTTGAAGCTA GAACTATTGA GAGAAACACG AAACCTTATA GGAAGGATGA GAATTCAATC 
    AATGGAACAC CAGATATCAC AGTAGAAATT AGAGAACATA TTCAGCTTAA TGAAAGTAAA 
    ATTGTTAAAC AAGCTGGAGT TGCCACAAAA GGGCCCAATG AATATATTCA AGAAATAGAA 
    TTTGAAAACT TGTCTCCAGG AAGTGTTATT ATATTCAGAG TTAGTCTTGA TCCACATGCA 
    CAAGTCGCTG TTGGAATTCT TCGAAATCAT CTGACACAAT TCAGTCCTCA CTTTAAATCT 
    GGCAGCCTAG CTGTTGACAA TGCAGATCCT ATATTAAAAA TTCCTTTTGC TTCTCTTGCC 
    TCCAGATTAA CTTTGGCTGA GCTAAATCAG ATCCTTTACC GATGTGAATC AGAAGAAAAG 
    GAAGATGGTG GAGGGTGCTA TGACATACCA AACTGGTCAG CCCTTAAATA TGCAGGTCTT 
    CAAGGTTTAA TGTCTGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCTTTT 
    TGTAATAATT TGAGATCTGG AGATTGGATG ATTGACTATG TCAGTAACCG GCTTATTTCA 
    CGATCAGGAA CTATTGCTGA AGTTGGTAAA TGGTTGCAGG CTATGTTCTT CTACCTGAAG 
    CAGATCCCAC GTTACCTTAT CCCATGTTAC TTTGATGCTA TATTAATTGG TGCATATACC 
    ACTCTTCTGG ATACAGCATG GAAGCAGATG TCAAGCTTTG TTCAGAATGG TTCAACCTTT 
    GTGAAACACC TTTCATTGGG TTCAGTTCAA CTGTGTGGAG TAGGAAAATT CCCTTCCCTG 
    CCAATTCTTT CACCTGCCCT AATGGATGTA CCTTATAGGT TAAATGAGAT CACAAAAGAA 
    AAGGAGCAAT GTTGTGTTTC TCTAGCTGCA GGCTTACCTC ATTTTTCTTC TGGTATTTTC 
    CGCTGCTGGG GAAGGGATAC TTTTATTGCA CTTAGAGGTA TACTGCTGAT TACTGGACGC 
    TATGTAGAAG CCAGGAATAT TATTTTAGCA TTTGCGGGTA CCCTGAGGCA TGGTCTCATT 
    CCTAATCTAC TGGGTGAAGG AATTTATGCC AGATACAATT GTCGGGATGC TGTGTGGTGG 
    TGGCTGCAGT GTATCCAGGA TTACTGTAAA ATGGTTCCAA ATGGTCTAGA CATTCTCAAG 
    TGCCCAGTTT CCAGAATGTA TCCTACAGAT GATTCTGCTC CTTTGCCTGC TGGCACACTG 
    GATCAGCCAT TGTTTGAAGT CATACAGGAA GCAATGCAAA AACACATGCA GGGCATACAG 
    TTCCGAGAAA GGAATGCTGG TCCCCAGATA GATCGAAACA TGAAGGACGA AGGTTTTAAT 
    ATAACTGCAG GAGTTGATGA AGAAACAGGA TTTGTTTATG GAGGAAATCG TTTCAATTGT 
    GGCACATGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAGAGG AATCCCAGCC 
    ACACCAAGAG ATGGGTCTGC TGTGGAAATT GTGGGCCTGA GTAAATCTGC TGTTCGCTGG 
    TTGCTGGAAT TATCCAAAAA AAATATTTTC CCTTATCATG AAGTCACAGT AAAAAGACAT 
    GGAAAGGCTA TAAAGGTCTC ATATGATGAG TGGAACAGAA AAATACAAGA CAACTTTGAA 
    AAGCTATTTC ATGTTTCCGA AGACCCTTCA GATTTAAATG AAAAGCATCC AAATCTGGTT 
    CACAAACGTG GCATATACAA AGATAGTTAT GGAGCTTCAA GTCCTTGGTG TGACTATCAG 
    CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTGGCCCCTG AGCTCTTTAC TACAGAAAAA 
    GCATGGAAAG CTTTGGAGAT TGCAGAAAAA AAATTGCTTG GTCCCCTTGG CATGAAAACT 
    TTAGATCCAG ATGATATGGT TTACTGTGGA ATTTATGACA ATGCATTAGA CAATGACAAC 
    TACAATCTTG CTAAAGGTTT CAATTATCAC CAAGGACCTG AGTGGCTGTG GCCTATTGGG 
    TATTTTCTTC GTGCAAAATT ATATTTTTCC AGATTGATGG GCCCGGAGAC TACTGCAAAG 
    ACTATAGTTT TGGTTAAAAA TGTTCTTTCC CGACATTATG TTCATCTTGA GAGATCCCCT 
    TGGAAAGGAC TTCCAGAACT GACCAATGAG AATGCCCAGT ACTGTCCTTT CAGCTGTGAA 
    ACACAAGCCT GGTCAATTGC TACTATTCTT GAGACACTTT ATGATTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000637.2
    CDS260..4810
    Translation

    Target ORF information:

    RefSeq Version NM_000646.2
    Organism Homo sapiens(human)
    Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000646.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCCCCAA TTCTGAGCAT TAATTTATTT ATTGGGTATG AGCTACAGTT CCGATTAGGC 
    CCAACTTTAC AGGGAAAAGC AGTTACCGTG TATACAAATT ACCCATTTCC TGGAGAAACA 
    TTTAATAGAG AAAAATTCCG TTCTCTGGAT TGGGAAAATC CAACAGAAAG AGAAGATGAT 
    TCTGATAAAT ACTGTAAACT TAATCTGCAA CAATCTGGTT CATTTCAGTA TTATTTCCTT 
    CAAGGAAATG AGAAAAGTGG TGGAGGTTAC ATAGTTGTGG ACCCCATTTT ACGTGTTGGT 
    GCTGATAATC ATGTGCTACC CTTGGACTGT GTTACTCTTC AGACATTTTT AGCTAAGTGT 
    TTGGGACCTT TTGATGAATG GGAAAGCAGA CTTAGGGTTG CAAAAGAATC AGGCTACAAC 
    ATGATTCATT TTACCCCATT GCAGACTCTT GGACTATCTA GGTCATGCTA CTCCCTTGCC 
    AATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTAATA GAAAGTATAC CTGGAATGAT 
    GTTGGACAGC TAGTGGAAAA ATTAAAAAAG GAATGGAATG TTATTTGTAT TACTGATGTT 
    GTCTACAATC ATACTGCTGC TAATAGTAAA TGGATCCAGG AACATCCAGA ATGTGCCTAT 
    AATCTTGTGA ATTCTCCACA CTTAAAACCT GCCTGGGTCT TAGACAGAGC ACTTTGGCGT 
    TTCTCCTGTG ATGTTGCAGA AGGGAAATAC AAAGAAAAGG GAATACCTGC TTTGATTGAA 
    AATGATCACC ATATGAATTC CATCCGAAAA ATAATTTGGG AGGATATTTT TCCAAAGCTT 
    AAACTCTGGG AATTTTTCCA AGTAGATGTC AACAAAGCGG TTGAGCAATT TAGAAGACTT 
    CTTACACAAG AAAATAGGCG AGTAACCAAG TCTGATCCAA ACCAACACCT TACGATTATT 
    CAAGATCCTG AATACAGACG GTTTGGCTGT ACTGTAGATA TGAACATTGC ACTAACGACT 
    TTCATACCAC ATGACAAGGG GCCAGCAGCA ATTGAAGAAT GCTGTAATTG GTTTCATAAA 
    AGAATGGAGG AATTAAATTC AGAGAAGCAT CGACTCATTA ACTATCATCA GGAACAGGCA 
    GTTAATTGCC TTTTGGGAAA TGTGTTTTAT GAACGACTGG CTGGCCATGG TCCAAAACTA 
    GGACCTGTCA CTAGAAAGCA TCCTTTAGTT ACCAGGTATT TTACTTTCCC ATTTGAAGAG 
    ATAGACTTCT CCATGGAAGA ATCTATGATT CATCTGCCAA ATAAAGCTTG TTTTCTGATG 
    GCACACAATG GATGGGTAAT GGGAGATGAT CCTCTTCGAA ACTTTGCTGA ACCGGGTTCA 
    GAAGTTTACC TAAGGAGAGA ACTTATTTGC TGGGGAGACA GTGTTAAATT ACGCTATGGG 
    AATAAACCAG AGGACTGTCC TTATCTCTGG GCACACATGA AAAAATACAC TGAAATAACT 
    GCAACTTATT TCCAGGGAGT ACGTCTTGAT AACTGCCACT CAACACCTCT TCACGTAGCT 
    GAGTACATGT TGGATGCTGC TAGGAATTTG CAACCCAATT TATATGTAGT AGCTGAACTG 
    TTCACAGGAA GTGAAGATCT GGACAATGTC TTTGTTACTA GACTGGGCAT TAGTTCCTTA 
    ATAAGAGAGG CAATGAGTGC ATATAATAGT CATGAAGAGG GCAGATTAGT TTACCGATAT 
    GGAGGAGAAC CTGTTGGATC CTTTGTTCAG CCCTGTTTGA GGCCTTTAAT GCCAGCTATT 
    GCACATGCCC TGTTTATGGA TATTACGCAT GATAATGAGT GTCCTATTGT GCATAGATCA 
    GCGTATGATG CTCTTCCAAG TACTACAATT GTTTCTATGG CATGTTGTGC TAGTGGAAGT 
    ACAAGAGGCT ATGATGAATT AGTGCCTCAT CAGATTTCAG TGGTTTCTGA AGAACGGTTT 
    TACACTAAGT GGAATCCTGA AGCATTGCCT TCAAACACAG GTGAAGTTAA TTTCCAAAGC 
    GGCATTATTG CAGCCAGGTG TGCTATCAGT AAACTTCATC AGGAGCTTGG AGCCAAGGGT 
    TTTATTCAGG TGTATGTGGA TCAAGTTGAT GAAGACATAG TGGCAGTAAC AAGACACTCA 
    CCTAGCATCC ATCAGTCTGT TGTGGCTGTA TCTAGAACTG CTTTCAGGAA TCCCAAGACT 
    TCATTTTACA GCAAGGAAGT GCCTCAAATG TGCATCCCTG GCAAAATTGA AGAAGTAGTT 
    CTTGAAGCTA GAACTATTGA GAGAAACACG AAACCTTATA GGAAGGATGA GAATTCAATC 
    AATGGAACAC CAGATATCAC AGTAGAAATT AGAGAACATA TTCAGCTTAA TGAAAGTAAA 
    ATTGTTAAAC AAGCTGGAGT TGCCACAAAA GGGCCCAATG AATATATTCA AGAAATAGAA 
    TTTGAAAACT TGTCTCCAGG AAGTGTTATT ATATTCAGAG TTAGTCTTGA TCCACATGCA 
    CAAGTCGCTG TTGGAATTCT TCGAAATCAT CTGACACAAT TCAGTCCTCA CTTTAAATCT 
    GGCAGCCTAG CTGTTGACAA TGCAGATCCT ATATTAAAAA TTCCTTTTGC TTCTCTTGCC 
    TCCAGATTAA CTTTGGCTGA GCTAAATCAG ATCCTTTACC GATGTGAATC AGAAGAAAAG 
    GAAGATGGTG GAGGGTGCTA TGACATACCA AACTGGTCAG CCCTTAAATA TGCAGGTCTT 
    CAAGGTTTAA TGTCTGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCTTTT 
    TGTAATAATT TGAGATCTGG AGATTGGATG ATTGACTATG TCAGTAACCG GCTTATTTCA 
    CGATCAGGAA CTATTGCTGA AGTTGGTAAA TGGTTGCAGG CTATGTTCTT CTACCTGAAG 
    CAGATCCCAC GTTACCTTAT CCCATGTTAC TTTGATGCTA TATTAATTGG TGCATATACC 
    ACTCTTCTGG ATACAGCATG GAAGCAGATG TCAAGCTTTG TTCAGAATGG TTCAACCTTT 
    GTGAAACACC TTTCATTGGG TTCAGTTCAA CTGTGTGGAG TAGGAAAATT CCCTTCCCTG 
    CCAATTCTTT CACCTGCCCT AATGGATGTA CCTTATAGGT TAAATGAGAT CACAAAAGAA 
    AAGGAGCAAT GTTGTGTTTC TCTAGCTGCA GGCTTACCTC ATTTTTCTTC TGGTATTTTC 
    CGCTGCTGGG GAAGGGATAC TTTTATTGCA CTTAGAGGTA TACTGCTGAT TACTGGACGC 
    TATGTAGAAG CCAGGAATAT TATTTTAGCA TTTGCGGGTA CCCTGAGGCA TGGTCTCATT 
    CCTAATCTAC TGGGTGAAGG AATTTATGCC AGATACAATT GTCGGGATGC TGTGTGGTGG 
    TGGCTGCAGT GTATCCAGGA TTACTGTAAA ATGGTTCCAA ATGGTCTAGA CATTCTCAAG 
    TGCCCAGTTT CCAGAATGTA TCCTACAGAT GATTCTGCTC CTTTGCCTGC TGGCACACTG 
    GATCAGCCAT TGTTTGAAGT CATACAGGAA GCAATGCAAA AACACATGCA GGGCATACAG 
    TTCCGAGAAA GGAATGCTGG TCCCCAGATA GATCGAAACA TGAAGGACGA AGGTTTTAAT 
    ATAACTGCAG GAGTTGATGA AGAAACAGGA TTTGTTTATG GAGGAAATCG TTTCAATTGT 
    GGCACATGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAGAGG AATCCCAGCC 
    ACACCAAGAG ATGGGTCTGC TGTGGAAATT GTGGGCCTGA GTAAATCTGC TGTTCGCTGG 
    TTGCTGGAAT TATCCAAAAA AAATATTTTC CCTTATCATG AAGTCACAGT AAAAAGACAT 
    GGAAAGGCTA TAAAGGTCTC ATATGATGAG TGGAACAGAA AAATACAAGA CAACTTTGAA 
    AAGCTATTTC ATGTTTCCGA AGACCCTTCA GATTTAAATG AAAAGCATCC AAATCTGGTT 
    CACAAACGTG GCATATACAA AGATAGTTAT GGAGCTTCAA GTCCTTGGTG TGACTATCAG 
    CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTGGCCCCTG AGCTCTTTAC TACAGAAAAA 
    GCATGGAAAG CTTTGGAGAT TGCAGAAAAA AAATTGCTTG GTCCCCTTGG CATGAAAACT 
    TTAGATCCAG ATGATATGGT TTACTGTGGA ATTTATGACA ATGCATTAGA CAATGACAAC 
    TACAATCTTG CTAAAGGTTT CAATTATCAC CAAGGACCTG AGTGGCTGTG GCCTATTGGG 
    TATTTTCTTC GTGCAAAATT ATATTTTTCC AGATTGATGG GCCCGGAGAC TACTGCAAAG 
    ACTATAGTTT TGGTTAAAAA TGTTCTTTCC CGACATTATG TTCATCTTGA GAGATCCCCT 
    TGGAAAGGAC TTCCAGAACT GACCAATGAG AATGCCCAGT ACTGTCCTTT CAGCTGTGAA 
    ACACAAGCCT GGTCAATTGC TACTATTCTT GAGACACTTT ATGATTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

見積依頼

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