agl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	agl
Entrez Gene ID	780065
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Synonyms	gde,xagl
General protein information	Preferred Names amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Names amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase glycogen debranching enzyme 6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) amylo-1, 6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031901014.1	XP_031756874.1	glycogen debranching enzyme isoform X1
XM_018093624.1	XP_017949113.1	glycogen debranching enzyme
XM_031901015.1	XP_031756875.1	glycogen debranching enzyme isoform X1
XM_031901017.1	XP_031756877.1	glycogen debranching enzyme isoform X2
XM_031901012.1	XP_031756872.1	glycogen debranching enzyme isoform X1
XM_031901016.1	XP_031756876.1	glycogen debranching enzyme isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Homo sapiens (human)	AGL	NP_000635.2
	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Gene Ontology

The following agl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa44199	XM_018093624.1 Latest version!	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa57297	XM_031901014.1 Latest version!	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa57297	XM_031901015.1 Latest version!	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa57297	XM_031901012.1 Latest version!	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa57297	XM_031901016.1 Latest version!	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa57298	XM_031901017.1 Latest version!	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

agl ( XM_018093624.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949113.1 Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), mRNA.

Price & Availability↑

CloneID	OXa44199
Clone ID Related Accession (Same CDS sequence)	XM_018093624.1
Accession Version	XM_018093624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GGAGATGCTG 
GCTGCAGCCA GGTCTGTCAG ACCAAATCTT TATGTGATTG CTGAGCTCTT CACTGGCAGC 
GAATACATCG ACAATGTGTT TGTCAACCGC CTGGGAATTA CAAGCCTCAT TCGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT AAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949113.1
CDS	565..5163
Translation	MEHSKQVRVLQLNDMEKLDKRLFRLEQGFELQFRLGPTLQGKDVTVYSNYPPPGAVYDRQTFHPLQWHNPTGREEDSDKFCKLDLKIAGSFQYHFLQGDQKNGEGYIVVDPVLRVGADNRALPLDCITLQTYLSKCLGPLDEWEDRLKVAKEAGYNMIHFTPLQTLGESRSCYSLADQLQLNPDFSRPGKVYGWDDVGKLVEKMRKEWNMLSITDVVYNHTAANSKWIQQHPECGYNLVNSPHLKPAWLLDRALWHLTCDAAEGKYAAKGLPALIKTDQQLNSIRGIVWEEIYPRIKLWEFYQVEVDKAVKQFRALLTSGAQKKGNKSDPKHLMIIQDPEYKRFGCSVDMDTALNTFIPHSNGPSAIEECCTWFRKRLEELNAEKHRQMNFHHEQAVNCILGTMTYERLAGQGPKLGPITKKDPIVTRYFTFPFKDMSLSEEESMMHQPDKACYFLAHNGWVMGDDPLRNFAGPDSNVYVRRELICWGDSVKLRYGDKPSDCPYLWEHMKKYTEITAKHFHGVRLDNCHSTPIHVAEEMLAAARSVRPNLYVIAELFTGSEYIDNVFVNRLGITSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPSLRPLVPSIAHALFMDVTHDNESPITHRSAYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWNPKMAAPIPRDVNGQSGIIAGKRALNKLHQELAARGFLQVYVDQVDEDIVAVTRHCPHTHQSVVAVSRTAFRDPKNSFYSKEVPQMCIPGKIEEVILEARTVERKAEPYSQDEKFINGLPQYTVEIREHIQLKESKIVKQAGITTKGPNEFVQEIDFENLTPGSVIIFRVSLDPKAQESVGILRSHLIQFTPHFTSGSLPDDQAHPILKVPFASIAAKLSLADLNQVLYRCDSEEQEDGGGCYNIPNWTPLKYAGLQGLISVMVDIRPKNDLGHPFCDNLRCGDWMIDYVSNRLIARGGPCAEVGKWFQAMFSYLKQIPRYLIPCYFDAILVGAYTTLLDAAWKQMSSFVQNGSTLIKHLALGSVQMCGVGRFPALPVLSPSLQDVPYRLNSITQEKEQCCPSLAAGLPHFSSGIFRCWGRDTFISLRGLLLITGSYLEARNIILAFAGTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYCITVPNGSAILQCPVSRMYPTDDSPTQPAGQVDQPLYEVIQEALQRHALGINFRERNAGPQIDRNMRDEGFNIVAGVDLETGFVFGGNRLNCGTWMDKMGESERARSKGIPATPRDGSAVEIVGLCKSTVRWLQDLSEKKLYPYKGVTVLRDGREVTLTFSEWNQRIQDNFEKKFFVSENISDPNEQRPDLVHKRGIYKDSFGASSPWCDYQLRPNFPVAMVVAPELFTPKNAWKALEITEKKLMGPLGMKTLDPDDMVYCGVYDNALDNDNYNLSKGFNYHQGPEWMWPIGYFLRAKLHFAKLMDRESYQKTVVLVKNVLSRHYTHLERSSWKGLPELTNENGQYCPFSCETQAWSISVILEVLYDL

Target ORF information:

RefSeq Version	XM_018093624.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093624.1

ORF Insert Sequence:

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GGAGATGCTG 
GCTGCAGCCA GGTCTGTCAG ACCAAATCTT TATGTGATTG CTGAGCTCTT CACTGGCAGC 
GAATACATCG ACAATGTGTT TGTCAACCGC CTGGGAATTA CAAGCCTCAT TCGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT AAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_031901014.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756874.1 Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa57297
Clone ID Related Accession (Same CDS sequence)	XM_031901014.1 , XM_031901015.1 , XM_031901012.1 , XM_031901016.1
Accession Version	XM_031901014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG 
GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT 
GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756874.1
CDS	188..4786
Translation	MEHSKQVRVLQLNDMEKLDKRLFRLEQGFELQFRLGPTLQGKDVTVYSNYPPPGAVYDRQTFHPLQWHNPTGREEDSDKFCKLDLKIAGSFQYHFLQGDQKNGEGYIVVDPVLRVGADNRALPLDCITLQTYLSKCLGPLDEWEDRLKVAKEAGYNMIHFTPLQTLGESRSCYSLADQLQLNPDFSRPGKVYGWDDVGKLVEKMRKEWNMLSITDVVYNHTAANSKWIQQHPECGYNLVNSPHLKPAWLLDRALWHLTCDAAEGKYAAKGLPALIKTDQQLNSIRGIVWEEIYPRIKLWEFYQVEVDKAVKQFRALLTSGAQKKGNKSDPKHLMIIQDPEYKRFGCSVDMDTALNTFIPHSNGPSAIEECCTWFRKRLEELNAEKHRQMNFHHEQAVNCILGTMTYERLAGQGPKLGPITKKDPIVTRYFTFPFKDMSLSEEESMMHQPDKACYFLAHNGWVMGDDPLRNFAGPDSNVYVRRELICWGDSVKLRYGDKPSDCPYLWEHMKKYTEITAKHFHGVRLDNCHSTPIHVAEYLLDVARKVRPDLYVVAELFTGSEELDNVFVTRLGINSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPSLRPLVPSIAHALFMDVTHDNESPITHRSAYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWNPKMAAPIPRDVNGQSGIIAGKRALNKLHQELAARGFLQVYVDQVDEDIVAVTRHCPHTHQSVVAVSRTAFRDPKNSFYSKEVPQMCIPGKIEEVILEARTVERKAEPYSQDEKFINGLPQYTVEIREHIQLKESKIVKQAGITTKGPNEFVQEIDFENLTPGSVIIFRVSLDPKAQESVGILRSHLIQFTPHFTSGSLPDDQAHPILKVPFASIAAKLSLADLNQVLYRCDSEEQEDGGGCYNIPNWTPLKYAGLQGLISVMVDIRPKNDLGHPFCDNLRCGDWMIDYVSNRLIARGGPCAEVGKWFQAMFSYLKQIPRYLIPCYFDAILVGAYTTLLDAAWKQMSSFVQNGSTLIKHLALGSVQMCGVGRFPALPVLSPSLQDVPYRLNSITQEKEQCCPSLAAGLPHFSSGIFRCWGRDTFISLRGLLLITGSYLEARNIILAFAGTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYCITVPNGSAILQCPVSRMYPTDDSPTQPAGQVDQPLYEVIQEALQRHALGINFRERNAGPQIDRNMRDEGFNIVAGVDLETGFVFGGNRLNCGTWMDKMGESERARSKGIPATPRDGSAVEIVGLCKSTVRWLQDLSEKKLYPYKGVTVLRDGREVTLTFSEWNQRIQDNFEKKFFVSENISDPNEQRPDLVHKRGIYKDSFGASSPWCDYQLRPNFPVAMVVAPELFTPKNAWKALEITEKKLMGPLGMKTLDPDDMVYCGVYDNALDNDNYNLSKGFNYHQGPEWMWPIGYFLRAKLHFAKLMDRESYQKTVVLVKNVLSRHYTHLERSSWKGLPELTNENGQYCPFSCETQAWSISVILEVLYDL

Target ORF information:

RefSeq Version	XM_031901014.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901014.1

ORF Insert Sequence:

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG 
GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT 
GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_031901015.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756875.1 Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.

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CloneID	OXa57297
Clone ID Related Accession (Same CDS sequence)	XM_031901014.1 , XM_031901015.1 , XM_031901012.1 , XM_031901016.1
Accession Version	XM_031901015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG 
GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT 
GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756875.1
CDS	516..5114
Translation	MEHSKQVRVLQLNDMEKLDKRLFRLEQGFELQFRLGPTLQGKDVTVYSNYPPPGAVYDRQTFHPLQWHNPTGREEDSDKFCKLDLKIAGSFQYHFLQGDQKNGEGYIVVDPVLRVGADNRALPLDCITLQTYLSKCLGPLDEWEDRLKVAKEAGYNMIHFTPLQTLGESRSCYSLADQLQLNPDFSRPGKVYGWDDVGKLVEKMRKEWNMLSITDVVYNHTAANSKWIQQHPECGYNLVNSPHLKPAWLLDRALWHLTCDAAEGKYAAKGLPALIKTDQQLNSIRGIVWEEIYPRIKLWEFYQVEVDKAVKQFRALLTSGAQKKGNKSDPKHLMIIQDPEYKRFGCSVDMDTALNTFIPHSNGPSAIEECCTWFRKRLEELNAEKHRQMNFHHEQAVNCILGTMTYERLAGQGPKLGPITKKDPIVTRYFTFPFKDMSLSEEESMMHQPDKACYFLAHNGWVMGDDPLRNFAGPDSNVYVRRELICWGDSVKLRYGDKPSDCPYLWEHMKKYTEITAKHFHGVRLDNCHSTPIHVAEYLLDVARKVRPDLYVVAELFTGSEELDNVFVTRLGINSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPSLRPLVPSIAHALFMDVTHDNESPITHRSAYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWNPKMAAPIPRDVNGQSGIIAGKRALNKLHQELAARGFLQVYVDQVDEDIVAVTRHCPHTHQSVVAVSRTAFRDPKNSFYSKEVPQMCIPGKIEEVILEARTVERKAEPYSQDEKFINGLPQYTVEIREHIQLKESKIVKQAGITTKGPNEFVQEIDFENLTPGSVIIFRVSLDPKAQESVGILRSHLIQFTPHFTSGSLPDDQAHPILKVPFASIAAKLSLADLNQVLYRCDSEEQEDGGGCYNIPNWTPLKYAGLQGLISVMVDIRPKNDLGHPFCDNLRCGDWMIDYVSNRLIARGGPCAEVGKWFQAMFSYLKQIPRYLIPCYFDAILVGAYTTLLDAAWKQMSSFVQNGSTLIKHLALGSVQMCGVGRFPALPVLSPSLQDVPYRLNSITQEKEQCCPSLAAGLPHFSSGIFRCWGRDTFISLRGLLLITGSYLEARNIILAFAGTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYCITVPNGSAILQCPVSRMYPTDDSPTQPAGQVDQPLYEVIQEALQRHALGINFRERNAGPQIDRNMRDEGFNIVAGVDLETGFVFGGNRLNCGTWMDKMGESERARSKGIPATPRDGSAVEIVGLCKSTVRWLQDLSEKKLYPYKGVTVLRDGREVTLTFSEWNQRIQDNFEKKFFVSENISDPNEQRPDLVHKRGIYKDSFGASSPWCDYQLRPNFPVAMVVAPELFTPKNAWKALEITEKKLMGPLGMKTLDPDDMVYCGVYDNALDNDNYNLSKGFNYHQGPEWMWPIGYFLRAKLHFAKLMDRESYQKTVVLVKNVLSRHYTHLERSSWKGLPELTNENGQYCPFSCETQAWSISVILEVLYDL

Target ORF information:

RefSeq Version	XM_031901015.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901015.1

ORF Insert Sequence:

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG 
GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT 
GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_031901012.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756872.1 Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.

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CloneID	OXa57297
Clone ID Related Accession (Same CDS sequence)	XM_031901014.1 , XM_031901015.1 , XM_031901012.1 , XM_031901016.1
Accession Version	XM_031901012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG 
GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT 
GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756872.1
CDS	174..4772
Translation	MEHSKQVRVLQLNDMEKLDKRLFRLEQGFELQFRLGPTLQGKDVTVYSNYPPPGAVYDRQTFHPLQWHNPTGREEDSDKFCKLDLKIAGSFQYHFLQGDQKNGEGYIVVDPVLRVGADNRALPLDCITLQTYLSKCLGPLDEWEDRLKVAKEAGYNMIHFTPLQTLGESRSCYSLADQLQLNPDFSRPGKVYGWDDVGKLVEKMRKEWNMLSITDVVYNHTAANSKWIQQHPECGYNLVNSPHLKPAWLLDRALWHLTCDAAEGKYAAKGLPALIKTDQQLNSIRGIVWEEIYPRIKLWEFYQVEVDKAVKQFRALLTSGAQKKGNKSDPKHLMIIQDPEYKRFGCSVDMDTALNTFIPHSNGPSAIEECCTWFRKRLEELNAEKHRQMNFHHEQAVNCILGTMTYERLAGQGPKLGPITKKDPIVTRYFTFPFKDMSLSEEESMMHQPDKACYFLAHNGWVMGDDPLRNFAGPDSNVYVRRELICWGDSVKLRYGDKPSDCPYLWEHMKKYTEITAKHFHGVRLDNCHSTPIHVAEYLLDVARKVRPDLYVVAELFTGSEELDNVFVTRLGINSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPSLRPLVPSIAHALFMDVTHDNESPITHRSAYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWNPKMAAPIPRDVNGQSGIIAGKRALNKLHQELAARGFLQVYVDQVDEDIVAVTRHCPHTHQSVVAVSRTAFRDPKNSFYSKEVPQMCIPGKIEEVILEARTVERKAEPYSQDEKFINGLPQYTVEIREHIQLKESKIVKQAGITTKGPNEFVQEIDFENLTPGSVIIFRVSLDPKAQESVGILRSHLIQFTPHFTSGSLPDDQAHPILKVPFASIAAKLSLADLNQVLYRCDSEEQEDGGGCYNIPNWTPLKYAGLQGLISVMVDIRPKNDLGHPFCDNLRCGDWMIDYVSNRLIARGGPCAEVGKWFQAMFSYLKQIPRYLIPCYFDAILVGAYTTLLDAAWKQMSSFVQNGSTLIKHLALGSVQMCGVGRFPALPVLSPSLQDVPYRLNSITQEKEQCCPSLAAGLPHFSSGIFRCWGRDTFISLRGLLLITGSYLEARNIILAFAGTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYCITVPNGSAILQCPVSRMYPTDDSPTQPAGQVDQPLYEVIQEALQRHALGINFRERNAGPQIDRNMRDEGFNIVAGVDLETGFVFGGNRLNCGTWMDKMGESERARSKGIPATPRDGSAVEIVGLCKSTVRWLQDLSEKKLYPYKGVTVLRDGREVTLTFSEWNQRIQDNFEKKFFVSENISDPNEQRPDLVHKRGIYKDSFGASSPWCDYQLRPNFPVAMVVAPELFTPKNAWKALEITEKKLMGPLGMKTLDPDDMVYCGVYDNALDNDNYNLSKGFNYHQGPEWMWPIGYFLRAKLHFAKLMDRESYQKTVVLVKNVLSRHYTHLERSSWKGLPELTNENGQYCPFSCETQAWSISVILEVLYDL

Target ORF information:

RefSeq Version	XM_031901012.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901012.1

ORF Insert Sequence:

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG 
GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT 
GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_031901016.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756876.1 Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.

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CloneID	OXa57297
Clone ID Related Accession (Same CDS sequence)	XM_031901014.1 , XM_031901015.1 , XM_031901012.1 , XM_031901016.1
Accession Version	XM_031901016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG 
GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT 
GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756876.1
CDS	309..4907
Translation	MEHSKQVRVLQLNDMEKLDKRLFRLEQGFELQFRLGPTLQGKDVTVYSNYPPPGAVYDRQTFHPLQWHNPTGREEDSDKFCKLDLKIAGSFQYHFLQGDQKNGEGYIVVDPVLRVGADNRALPLDCITLQTYLSKCLGPLDEWEDRLKVAKEAGYNMIHFTPLQTLGESRSCYSLADQLQLNPDFSRPGKVYGWDDVGKLVEKMRKEWNMLSITDVVYNHTAANSKWIQQHPECGYNLVNSPHLKPAWLLDRALWHLTCDAAEGKYAAKGLPALIKTDQQLNSIRGIVWEEIYPRIKLWEFYQVEVDKAVKQFRALLTSGAQKKGNKSDPKHLMIIQDPEYKRFGCSVDMDTALNTFIPHSNGPSAIEECCTWFRKRLEELNAEKHRQMNFHHEQAVNCILGTMTYERLAGQGPKLGPITKKDPIVTRYFTFPFKDMSLSEEESMMHQPDKACYFLAHNGWVMGDDPLRNFAGPDSNVYVRRELICWGDSVKLRYGDKPSDCPYLWEHMKKYTEITAKHFHGVRLDNCHSTPIHVAEYLLDVARKVRPDLYVVAELFTGSEELDNVFVTRLGINSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPSLRPLVPSIAHALFMDVTHDNESPITHRSAYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWNPKMAAPIPRDVNGQSGIIAGKRALNKLHQELAARGFLQVYVDQVDEDIVAVTRHCPHTHQSVVAVSRTAFRDPKNSFYSKEVPQMCIPGKIEEVILEARTVERKAEPYSQDEKFINGLPQYTVEIREHIQLKESKIVKQAGITTKGPNEFVQEIDFENLTPGSVIIFRVSLDPKAQESVGILRSHLIQFTPHFTSGSLPDDQAHPILKVPFASIAAKLSLADLNQVLYRCDSEEQEDGGGCYNIPNWTPLKYAGLQGLISVMVDIRPKNDLGHPFCDNLRCGDWMIDYVSNRLIARGGPCAEVGKWFQAMFSYLKQIPRYLIPCYFDAILVGAYTTLLDAAWKQMSSFVQNGSTLIKHLALGSVQMCGVGRFPALPVLSPSLQDVPYRLNSITQEKEQCCPSLAAGLPHFSSGIFRCWGRDTFISLRGLLLITGSYLEARNIILAFAGTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYCITVPNGSAILQCPVSRMYPTDDSPTQPAGQVDQPLYEVIQEALQRHALGINFRERNAGPQIDRNMRDEGFNIVAGVDLETGFVFGGNRLNCGTWMDKMGESERARSKGIPATPRDGSAVEIVGLCKSTVRWLQDLSEKKLYPYKGVTVLRDGREVTLTFSEWNQRIQDNFEKKFFVSENISDPNEQRPDLVHKRGIYKDSFGASSPWCDYQLRPNFPVAMVVAPELFTPKNAWKALEITEKKLMGPLGMKTLDPDDMVYCGVYDNALDNDNYNLSKGFNYHQGPEWMWPIGYFLRAKLHFAKLMDRESYQKTVVLVKNVLSRHYTHLERSSWKGLPELTNENGQYCPFSCETQAWSISVILEVLYDL

Target ORF information:

RefSeq Version	XM_031901016.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901016.1

ORF Insert Sequence:

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GTACTTGCTG 
GATGTTGCTA GAAAAGTCCG CCCGGATTTG TATGTAGTGG CTGAACTATT CACTGGAAGT 
GAAGAGCTGG ACAATGTGTT TGTCACTAGA CTGGGCATTA ACTCACTCAT ACGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_031901017.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756877.1 Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.

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CloneID	OXa57298
Clone ID Related Accession (Same CDS sequence)	XM_031901017.1
Accession Version	XM_031901017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GGAGATGCTG 
GCTGCAGCCA GGTCTGTCAG ACCAAATCTT TATGTGATTG CTGAGCTCTT CACTGGCAGC 
GAATACATCG ACAATGTGTT TGTCAACCGC CTGGGAATTA CAAGCCTCAT TCGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756877.1
CDS	174..4772
Translation	MEHSKQVRVLQLNDMEKLDKRLFRLEQGFELQFRLGPTLQGKDVTVYSNYPPPGAVYDRQTFHPLQWHNPTGREEDSDKFCKLDLKIAGSFQYHFLQGDQKNGEGYIVVDPVLRVGADNRALPLDCITLQTYLSKCLGPLDEWEDRLKVAKEAGYNMIHFTPLQTLGESRSCYSLADQLQLNPDFSRPGKVYGWDDVGKLVEKMRKEWNMLSITDVVYNHTAANSKWIQQHPECGYNLVNSPHLKPAWLLDRALWHLTCDAAEGKYAAKGLPALIKTDQQLNSIRGIVWEEIYPRIKLWEFYQVEVDKAVKQFRALLTSGAQKKGNKSDPKHLMIIQDPEYKRFGCSVDMDTALNTFIPHSNGPSAIEECCTWFRKRLEELNAEKHRQMNFHHEQAVNCILGTMTYERLAGQGPKLGPITKKDPIVTRYFTFPFKDMSLSEEESMMHQPDKACYFLAHNGWVMGDDPLRNFAGPDSNVYVRRELICWGDSVKLRYGDKPSDCPYLWEHMKKYTEITAKHFHGVRLDNCHSTPIHVAEEMLAAARSVRPNLYVIAELFTGSEYIDNVFVNRLGITSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPSLRPLVPSIAHALFMDVTHDNESPITHRSAYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWNPKMAAPIPRDVNGQSGIIAGKRALNKLHQELAARGFLQVYVDQVDEDIVAVTRHCPHTHQSVVAVSRTAFRDPKNSFYSKEVPQMCIPGKIEEVILEARTVERKAEPYSQDEKFINGLPQYTVEIREHIQLKESKIVKQAGITTKGPNEFVQEIDFENLTPGSVIIFRVSLDPKAQESVGILRSHLIQFTPHFTSGSLPDDQAHPILKVPFASIAAKLSLADLNQVLYRCDSEEQEDGGGCYNIPNWTPLKYAGLQGLISVMVDIRPKNDLGHPFCDNLRCGDWMIDYVSNRLIARGGPCAEVGKWFQAMFSYLKQIPRYLIPCYFDAILVGAYTTLLDAAWKQMSSFVQNGSTLIKHLALGSVQMCGVGRFPALPVLSPSLQDVPYRLNSITQEKEQCCPSLAAGLPHFSSGIFRCWGRDTFISLRGLLLITGSYLEARNIILAFAGTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYCITVPNGSAILQCPVSRMYPTDDSPTQPAGQVDQPLYEVIQEALQRHALGINFRERNAGPQIDRNMRDEGFNIVAGVDLETGFVFGGNRLNCGTWMDKMGESERARSKGIPATPRDGSAVEIVGLCKSTVRWLQDLSEKKLYPYKGVTVLRDGREVTLTFSEWNQRIQDNFEKKFFVSENISDPNEQRPDLVHKRGIYKDSFGASSPWCDYQLRPNFPVAMVVAPELFTPKNAWKALEITEKKLMGPLGMKTLDPDDMVYCGVYDNALDNDNYNLSKGFNYHQGPEWMWPIGYFLRAKLHFAKLMDRESYQKTVVLVKNVLSRHYTHLERSSWKGLPELTNENGQYCPFSCETQAWSISVILEVLYDL

Target ORF information:

RefSeq Version	XM_031901017.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901017.1

ORF Insert Sequence:

ATGGAGCACA GTAAGCAGGT CCGGGTTCTG CAGCTTAATG ATATGGAGAA GTTAGACAAG 
AGACTTTTCA GACTTGAACA AGGGTTTGAG CTTCAGTTCA GGCTTGGCCC CACTCTGCAA 
GGAAAAGATG TCACCGTATA CTCAAACTAC CCACCCCCTG GTGCTGTTTA TGATCGTCAG 
ACCTTCCACC CCCTGCAGTG GCACAATCCA ACAGGTCGAG AGGAAGATTC TGATAAGTTC 
TGTAAACTGG ACCTCAAAAT TGCTGGTTCA TTCCAGTACC ATTTCCTGCA GGGTGATCAG 
AAGAATGGGG AGGGATACAT TGTAGTAGAT CCAGTGCTCC GGGTTGGAGC TGATAATCGA 
GCGCTCCCTC TTGACTGCAT CACCCTTCAG ACTTACCTGT CCAAGTGCTT GGGGCCATTG 
GATGAATGGG AGGACAGACT GAAGGTGGCC AAGGAGGCAG GTTACAACAT GATACATTTC 
ACACCGCTAC AGACACTTGG AGAATCTCGT TCCTGTTATT CTCTTGCTGA CCAGCTGCAG 
CTAAATCCTG ACTTCTCCCG GCCTGGGAAA GTATATGGTT GGGATGATGT TGGAAAACTG 
GTGGAGAAGA TGAGAAAAGA GTGGAATATG CTAAGTATCA CAGATGTGGT TTATAATCAC 
ACAGCTGCCA ACAGCAAGTG GATTCAACAG CACCCTGAAT GTGGATACAA CCTTGTGAAT 
TCTCCTCACC TTAAGCCAGC ATGGCTTCTT GATCGGGCCC TGTGGCACCT GACTTGTGAT 
GCGGCTGAGG GAAAGTATGC AGCAAAAGGG CTTCCGGCAC TGATTAAAAC TGACCAGCAA 
CTCAATAGTA TCCGGGGTAT TGTGTGGGAA GAAATCTATC CCAGAATCAA GCTATGGGAG 
TTTTACCAGG TTGAAGTTGA TAAAGCAGTG AAACAGTTTC GAGCTCTGCT CACCTCAGGT 
GCACAGAAGA AAGGTAACAA GTCTGATCCT AAGCACTTGA TGATCATTCA GGATCCTGAA 
TACAAGCGAT TTGGGTGCTC TGTTGATATG GACACAGCTC TCAACACATT TATACCACAC 
AGTAATGGAC CCTCTGCAAT TGAGGAATGC TGCACCTGGT TCCGCAAGAG ACTGGAGGAG 
CTGAACGCAG AGAAACATAG ACAAATGAAC TTTCACCATG AACAGGCAGT AAACTGCATT 
TTGGGTACCA TGACTTATGA ACGATTGGCT GGTCAGGGTC CAAAGCTTGG CCCCATCACG 
AAGAAAGACC CCATAGTGAC CAGATATTTT ACCTTTCCAT TCAAAGATAT GTCTCTGTCT 
GAAGAAGAGT CTATGATGCA CCAGCCTGAT AAAGCTTGTT ACTTTTTGGC TCACAACGGC 
TGGGTAATGG GTGATGACCC ACTGCGGAAC TTTGCTGGAC CAGATTCCAA TGTTTACGTT 
CGAAGAGAGT TAATCTGCTG GGGAGACAGT GTTAAACTAA GATATGGTGA CAAACCATCT 
GACTGTCCAT ATCTCTGGGA ACATATGAAG AAGTACACAG AAATTACAGC CAAACATTTC 
CACGGAGTAC GTCTGGACAA CTGTCACTCC ACCCCCATCC ATGTGGCAGA GGAGATGCTG 
GCTGCAGCCA GGTCTGTCAG ACCAAATCTT TATGTGATTG CTGAGCTCTT CACTGGCAGC 
GAATACATCG ACAATGTGTT TGTCAACCGC CTGGGAATTA CAAGCCTCAT TCGAGAGGCC 
ATGAGTGCGT ATAACAGCCA TGAAGAAGGC CGACTGGTGT ATCGCTTTGG TGGGGAGCCT 
GTAGGATCGT TTGTCCAGCC CTCTCTTAGA CCGCTGGTTC CAAGTATCGC TCATGCACTT 
TTCATGGATG TCACGCATGA CAATGAAAGT CCTATTACAC ATCGATCTGC CTATGATTCA 
CTGCCCAGTT CTGCCATTGT TTCCATGGCT TGCTGTGCTA CAGGAAGCAC CCGTGGTTAT 
GATGAATTGG TACCACATCA GATCTCTGTG GTATCTGAAG AGAGGTTTTA TACAAAATGG 
AACCCAAAGA TGGCTGCTCC TATCCCAAGA GATGTGAATG GGCAAAGTGG GATCATTGCT 
GGAAAGAGAG CTCTAAACAA ACTTCACCAG GAGTTAGCAG CTCGTGGATT CCTGCAGGTG 
TATGTGGATC AGGTGGATGA GGATATAGTT GCAGTGACAC GTCATTGTCC ACATACACAC 
CAGTCTGTGG TGGCGGTGTC CAGAACAGCC TTTAGAGACC CTAAAAACTC TTTCTATAGC 
AAGGAAGTAC CACAGATGTG TATTCCAGGA AAAATAGAGG AAGTGATTCT AGAAGCAAGA 
ACAGTTGAGC GGAAAGCTGA GCCATATTCA CAAGATGAGA AATTCATTAA TGGCCTGCCC 
CAATATACAG TGGAGATCAG GGAGCACATC CAGCTGAAAG AAAGCAAGAT TGTAAAACAA 
GCTGGCATCA CAACCAAAGG ACCAAATGAA TTTGTTCAGG AAATAGACTT CGAGAATCTG 
ACCCCTGGAA GTGTGATTAT TTTCAGGGTA AGTTTGGACC CTAAAGCCCA AGAATCAGTA 
GGCATCCTGC GTAGTCACCT GATCCAGTTT ACACCACACT TCACCTCTGG AAGTTTGCCA 
GATGACCAGG CTCATCCTAT CCTGAAGGTG CCTTTTGCAT CTATTGCTGC AAAACTGTCT 
CTAGCTGATC TTAATCAAGT ACTGTACCGC TGTGATTCGG AGGAACAGGA AGATGGGGGT 
GGCTGCTACA ATATCCCAAA CTGGACCCCA CTAAAGTATG CTGGTCTACA AGGTTTAATA 
TCTGTGATGG TGGACATCAG ACCAAAGAAT GATCTTGGGC ATCCCTTCTG CGACAATCTG 
AGGTGTGGGG ATTGGATGAT TGATTATGTC AGTAACCGTC TGATTGCACG TGGAGGGCCA 
TGCGCAGAAG TGGGTAAATG GTTTCAGGCT ATGTTCTCCT ATTTGAAGCA GATCCCACGT 
TATCTCATTC CATGTTATTT TGATGCCATC CTGGTGGGGG CATACACAAC CCTCCTAGAT 
GCTGCATGGA AACAGATGTC CAGCTTTGTT CAGAATGGAT CCACCCTCAT TAAACACTTG 
GCCCTTGGAT CTGTGCAGAT GTGTGGAGTG GGAAGGTTCC CTGCCTTACC TGTGCTATCA 
CCCTCTCTGC AAGATGTCCC ATACCGCCTT AACAGCATTA CACAGGAAAA GGAGCAGTGC 
TGTCCCTCTT TAGCTGCTGG TCTTCCTCAT TTCTCTTCTG GAATTTTCCG ATGCTGGGGA 
AGGGATACAT TCATATCCCT GAGAGGATTG TTGTTAATCA CTGGAAGCTA CCTTGAAGCC 
AGGAACATTA TCCTGGCATT TGCTGGAACT CTGAGACATG GGCTAATCCC TAACCTTTTG 
GGTCAGGGGA CATATGCTAG ATACAACTGT CGTGATGCTG TGTGGTGGTG GTTGCAATGT 
ATACAGGATT ACTGTATCAC TGTTCCAAAT GGTTCTGCAA TCTTACAGTG CCCTGTCTCC 
AGGATGTACC CAACAGATGA TTCCCCCACA CAACCAGCAG GACAAGTGGA TCAGCCATTG 
TATGAAGTTA TACAGGAGGC TTTGCAGCGC CATGCTCTGG GCATCAACTT CCGGGAGAGG 
AACGCTGGCC CTCAGATAGA CAGAAACATG AGAGATGAAG GCTTTAATAT CGTTGCTGGA 
GTAGATCTGG AAACTGGTTT TGTATTTGGA GGAAACAGAT TAAATTGTGG GACATGGATG 
GACAAAATGG GGGAAAGCGA GAGAGCTCGA AGTAAAGGAA TCCCAGCAAC TCCAAGAGAT 
GGCTCTGCTG TGGAAATTGT TGGCCTTTGT AAATCCACTG TGCGTTGGCT TCAGGATCTG 
AGTGAAAAGA AGCTGTATCC ATACAAAGGA GTGACTGTGC TAAGGGATGG TAGAGAAGTG 
ACATTGACTT TCTCTGAGTG GAACCAGCGC ATCCAAGATA ATTTTGAGAA GAAGTTCTTT 
GTATCTGAAA ATATTTCTGA TCCCAATGAG CAGCGCCCAG ACCTTGTCCA TAAGCGTGGT 
ATCTACAAGG ACAGCTTTGG AGCTTCCAGC CCCTGGTGTG ATTACCAGCT CCGACCCAAC 
TTCCCTGTGG CCATGGTTGT GGCCCCAGAA CTGTTCACTC CAAAGAATGC TTGGAAAGCT 
CTCGAGATAA CAGAGAAAAA ACTAATGGGC CCGTTGGGAA TGAAGACACT GGACCCAGAT 
GACATGGTTT ATTGTGGAGT GTACGACAAT GCTTTGGACA ATGACAATTA CAACTTGTCA 
AAGGGATTTA ATTATCATCA AGGACCGGAA TGGATGTGGC CCATAGGATA TTTCCTGCGT 
GCCAAGTTAC ATTTTGCAAA ACTGATGGAT CGAGAATCAT ACCAGAAAAC TGTGGTTTTG 
GTGAAGAATG TCCTCTCTCG CCATTATACA CACTTAGAAA GATCTTCCTG GAAAGGCCTC 
CCAGAACTAA CCAATGAGAA TGGTCAGTAT TGCCCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCAATCTCCG TAATTTTGGA GGTCCTGTAT GACTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

agl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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