AGL cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGL
Entrez Gene ID	469392
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
General protein information	Preferred Names amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Names glycogen debranching enzyme amylo-1, 6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III)
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001159352.3	XP_001159352.1	glycogen debranching enzyme
XM_001159306.4	XP_001159306.1	glycogen debranching enzyme
XM_016923141.2	XP_016778630.1	glycogen debranching enzyme
XM_001159400.4	XP_001159400.1	glycogen debranching enzyme
XM_001159306.3	XP_001159306.1	glycogen debranching enzyme
XM_001159352.4	XP_001159352.1	glycogen debranching enzyme
XM_001159400.5	XP_001159400.1	glycogen debranching enzyme
XM_016923141.1	XP_016778630.1	glycogen debranching enzyme

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Homo sapiens (human)	AGL	NP_000635.2
	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Gene Ontology

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc02917	XM_001159400.5 Latest version!	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc02917	XM_001159306.4 Latest version!	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc02917	XM_001159352.4 Latest version!	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc02917	XM_016923141.2 Latest version!	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc59900	XM_001159400.4 Latest version!	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc59900	XM_001159352.3 Latest version!	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc59900	XM_001159306.3 Latest version!	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc59900	XM_016923141.1 Latest version!	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGL ( XM_001159306.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001159306.1 Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc02917
Clone ID Related Accession (Same CDS sequence)	XM_001159306.4 , XM_016923141.2 , XM_001159352.4 , XM_001159400.5
Accession Version	XM_001159306.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001159306.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001159306.1
CDS	199..4797
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLHFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_001159306.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001159306.4

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_016923141.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016778630.1 Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

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CloneID	OPc02917
Clone ID Related Accession (Same CDS sequence)	XM_001159306.4 , XM_016923141.2 , XM_001159352.4 , XM_001159400.5
Accession Version	XM_016923141.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016923141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016778630.1
CDS	243..4841
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLHFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_016923141.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923141.2

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_001159352.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001159352.1 Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc02917
Clone ID Related Accession (Same CDS sequence)	XM_001159306.4 , XM_016923141.2 , XM_001159352.4 , XM_001159400.5
Accession Version	XM_001159352.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001159352.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001159352.1
CDS	401..4999
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLHFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_001159352.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001159352.4

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_001159400.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001159400.1 Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

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CloneID	OPc02917
Clone ID Related Accession (Same CDS sequence)	XM_001159306.4 , XM_016923141.2 , XM_001159352.4 , XM_001159400.5
Accession Version	XM_001159400.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001159400.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001159400.1
CDS	479..5077
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLHFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_001159400.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001159400.5

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCACTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_001159352.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001159352.1 Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

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CloneID	OPc59900
Clone ID Related Accession (Same CDS sequence)	XM_001159352.3 , XM_001159400.4 , XM_001159306.3 , XM_016923141.1
Accession Version	XM_001159352.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001159352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001159352.1
CDS	401..4999
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLHFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_001159352.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001159352.3

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_001159400.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001159400.1 Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc59900
Clone ID Related Accession (Same CDS sequence)	XM_001159352.3 , XM_001159400.4 , XM_001159306.3 , XM_016923141.1
Accession Version	XM_001159400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001159400.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001159400.1
CDS	479..5077
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLHFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_001159400.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001159400.4

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_001159306.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001159306.1 Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc59900
Clone ID Related Accession (Same CDS sequence)	XM_001159352.3 , XM_001159400.4 , XM_001159306.3 , XM_016923141.1
Accession Version	XM_001159306.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001159306.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001159306.1
CDS	199..4797
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLHFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_001159306.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001159306.3

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_016923141.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016778630.1 Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

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CloneID	OPc59900
Clone ID Related Accession (Same CDS sequence)	XM_001159352.3 , XM_001159400.4 , XM_001159306.3 , XM_016923141.1
Accession Version	XM_016923141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016778630.1
CDS	243..4841
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASIASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLHFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_016923141.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923141.1

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAAAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAA CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAA 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACATCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTCCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAGC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACCAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCAGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAC ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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AGL cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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