DYSF gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015473390.1	XP_015328876.1	dysferlin isoform X2
XM_015473397.1	XP_015328883.1	dysferlin isoform X8
XM_015473399.1	XP_015328885.1	dysferlin isoform X11
XM_005212484.3	XP_005212541.1	dysferlin isoform X12
XM_005212485.3	XP_005212542.1	dysferlin isoform X13
XM_005212488.3	XP_005212545.1	dysferlin isoform X15
XM_005212492.3	XP_005212549.1	dysferlin isoform X19
XM_005212494.3	XP_005212551.1	dysferlin isoform X21
XM_015473402.1	XP_015328888.1	dysferlin isoform X26
XM_015473391.1	XP_015328877.1	dysferlin isoform X3
XM_015473394.1	XP_015328880.1	dysferlin isoform X5
XM_015473395.1	XP_015328881.1	dysferlin isoform X6
XM_005212489.3	XP_005212546.1	dysferlin isoform X16
XM_005212498.3	XP_005212555.1	dysferlin isoform X25
XM_015473392.1	XP_015328878.1	dysferlin isoform X4
XM_015473398.1	XP_015328884.1	dysferlin isoform X9
XM_010809847.2	XP_010808149.1	dysferlin isoform X10
XM_005212490.3	XP_005212547.1	dysferlin isoform X17
XM_005212491.3	XP_005212548.1	dysferlin isoform X18
XM_005212493.3	XP_005212550.1	dysferlin isoform X20
XM_005212496.3	XP_005212553.1	dysferlin isoform X23
XM_005212487.3	XP_005212544.1	dysferlin isoform X14
XM_005212495.3	XP_005212552.1	dysferlin isoform X22
NM_001102490.2	NP_001095960.1	dysferlin
XM_015473389.1	XP_015328875.1	dysferlin isoform X1
XM_015473396.1	XP_015328882.1	dysferlin isoform X7
XM_005212497.3	XP_005212554.1	dysferlin isoform X24

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa91845	XM_015473402.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa91835	XM_015473389.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91836	XM_015473390.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91837	XM_015473391.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91838	XM_015473392.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91839	XM_015473394.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91840	XM_015473395.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91841	XM_015473396.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91842	XM_015473397.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91843	XM_015473398.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91844	XM_015473399.1 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa01022	NM_001102490.2 Latest version!	Bos taurus dysferlin (DYSF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23809	XM_005212484.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23810	XM_005212485.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23812	XM_005212487.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23813	XM_005212488.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23814	XM_005212489.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23815	XM_005212490.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23816	XM_005212491.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23817	XM_005212492.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23818	XM_005212493.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23819	XM_005212494.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23820	XM_005212495.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23821	XM_005212496.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23822	XM_005212497.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23823	XM_005212498.3 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa47068	XM_010809847.2 Latest version!	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

DYSF ( XM_015473402.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328888.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OBa91845
Clone ID Related Accession (Same CDS sequence)	XM_015473402.1
Accession Version	XM_015473402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTCC TCTTTCCAAT CCCAAGACCA GAGACCAAGC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGC TGTGTGCACC TGCCTTCTTC CTCCAGGGCC TCCTGCCAAC GTCAGAGGAC 
ACGGACCTGC CCTACCCACC GCCCCAGAGG GAGGCCAACA TCTACATGGT CCCCCAGAAC 
ATCAAGCCGG TTCTCCAGCG AACCGCCATC GAGATCCTGG CGTGGGGTCT TCGGAACATG 
AAGAGCTACC AGCTGGCCAG CGTGTCCTCC CCGAGCCTGG TGGTGGAGTG CGGGGGCCAG 
TCTGTACAAT CCTGTGTCAT CAAGAACCTC CGGAAGAACC CCAACTTCGA CATCTGCACC 
CTCTTCATGG AAGTGATGCT GCCCAGGGAG GAGCTGTACT GTCCCCCCAT CGTGGTCAAG 
GTCATTGATA ACCGCCAGTT TGGCCGCCGG CCCGTGGTGG GCCAGTGTAC CATCCGCTCC 
CTGGAGGGCT TCCTGTGTGA TCCTTACTCA GAGGAGAGTC CGTCCCCACA GGGTGGCCCA 
GATGACGTGA GCTTACTCAG TCCTGGGGAA GATGTGCTGA TTGACATCGA TGACAAGGAG 
CCCCTCATCC CCATCCAGTT TGCAGATGGT CTCTCGGGCT TGGCCCCCAC TAACATGGCA 
TCTTCTCCAT CCAGTCTTCA TAATCAGAGC AGGCTCCTGG GAGATCCCTT AGTTGACCGC 
CTCATACAGA GGAGGAAAGC AGGCCCCAGA ATGGGCTTCC TGACGCCTTG TCCAGTGCCC 
TTCCCATGGA ACTGGGAGGA GGAGTTCATT GATTGGTGGA GCAAATTCTT TGCTTCAATT 
GGGGAGAGCG AAAAGTGCGG GTCCTACCTA GAGAAGGACT TTGACACTCT AAAGGTCTAT 
GACACATCCC TGGAGAATGT GAAGGCCTTT GAGGGCCTGT CTGACTTTTG TAATACATTC 
AAGCTCTACC GGGGCAAGAC ACAGGAGGAG ACTGAAGACC CATCAGTCAT TGGGGAGTTT 
AAGGGACTCT TCAAAATTTA TCCTCTCCCA GAAGACCCAG CCATCCCCTT GCCCCCGAGG 
CAGTTCCACC AGCTGGCTTC CCAGGGGCCC CAGGAATGCC TGGTCCGCGT CTACATTATC 
CGGGCGTTTG GCCTGCAGCC CAAGGATCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCGGT GAGTGACCAG GACAGCTACA TCCCTTGTAC TCTGGAGCCT 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC 
ACCCTCTATG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGGGAGAC GGTCATTGAC 
CTGGAGAACA GGCTGCTGTC TAAGTTCGGG GCCCGCTGTG GACTCCCCCA GACCTACTGC 
GTCTCTGGAC CCAACCAGTG GAGGGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTGTTC 
TGCCAGCAGC ACCGAGTCAA GGCCCCCGTG TACCGGACGG ACCGCGTGGT GTTTCAGGAT 
AAAGAATACA CCGTCGAAGA AATAGAGGCT GGCAGGGTCC CGAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCAGG GGCTGATCCC TGAGCACGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCGGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGGCCGGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACCAAGGA TGTGATCCTG 
GATGACCTCA GCATCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT 
GGCTTTGAAG AACACAAGCA AAAGACAGAT GTACATTATC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCGT CTTCCCTTTT GACTACCTGC CAGCTGAGCA GGTCTGCACC 
GTCTCCAAGA AGGATGCATT CTGGAGGCTG GACAAGACGG AGAGCAAGAT CCCTGCACGA 
GTGGTATTCC AGATCTGGGA CAACGACAAG TTCTCCTTCG ATGACTTTCT GGGCTCTCTG 
CAGCTGGACC TTAACCACAT GCCCAAGCCC GCCAAGACAG CTGAGAAGTG CTCTGCGGAC 
CAGCTGGAAG ACACCTTCCA CCCAGAGCGG TTTGTGTCTC TTTTTGAACA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAGATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCAGC CGGCCACGGC 
CGGGATGAAC CTAACATGAA CCCCAAGCTC GAGGACCCAA GGCGGCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGCTGT 
GCCATCATCT TCTTCCTCAT TCTCTTCATC TTCCTGCTCT TCTTGGGCAT CTTTGTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328888.1
CDS	1940..7387
Translation	MLLLFPIPRPETKLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEELCAPAFFLQGLLPTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHNQSRLLGDPLVDRLIQRRKAGPRMGFLTPCPVPFPWNWEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473402.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473402.1

ORF Insert Sequence:

ATGCTTCTCC TCTTTCCAAT CCCAAGACCA GAGACCAAGC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGC TGTGTGCACC TGCCTTCTTC CTCCAGGGCC TCCTGCCAAC GTCAGAGGAC 
ACGGACCTGC CCTACCCACC GCCCCAGAGG GAGGCCAACA TCTACATGGT CCCCCAGAAC 
ATCAAGCCGG TTCTCCAGCG AACCGCCATC GAGATCCTGG CGTGGGGTCT TCGGAACATG 
AAGAGCTACC AGCTGGCCAG CGTGTCCTCC CCGAGCCTGG TGGTGGAGTG CGGGGGCCAG 
TCTGTACAAT CCTGTGTCAT CAAGAACCTC CGGAAGAACC CCAACTTCGA CATCTGCACC 
CTCTTCATGG AAGTGATGCT GCCCAGGGAG GAGCTGTACT GTCCCCCCAT CGTGGTCAAG 
GTCATTGATA ACCGCCAGTT TGGCCGCCGG CCCGTGGTGG GCCAGTGTAC CATCCGCTCC 
CTGGAGGGCT TCCTGTGTGA TCCTTACTCA GAGGAGAGTC CGTCCCCACA GGGTGGCCCA 
GATGACGTGA GCTTACTCAG TCCTGGGGAA GATGTGCTGA TTGACATCGA TGACAAGGAG 
CCCCTCATCC CCATCCAGTT TGCAGATGGT CTCTCGGGCT TGGCCCCCAC TAACATGGCA 
TCTTCTCCAT CCAGTCTTCA TAATCAGAGC AGGCTCCTGG GAGATCCCTT AGTTGACCGC 
CTCATACAGA GGAGGAAAGC AGGCCCCAGA ATGGGCTTCC TGACGCCTTG TCCAGTGCCC 
TTCCCATGGA ACTGGGAGGA GGAGTTCATT GATTGGTGGA GCAAATTCTT TGCTTCAATT 
GGGGAGAGCG AAAAGTGCGG GTCCTACCTA GAGAAGGACT TTGACACTCT AAAGGTCTAT 
GACACATCCC TGGAGAATGT GAAGGCCTTT GAGGGCCTGT CTGACTTTTG TAATACATTC 
AAGCTCTACC GGGGCAAGAC ACAGGAGGAG ACTGAAGACC CATCAGTCAT TGGGGAGTTT 
AAGGGACTCT TCAAAATTTA TCCTCTCCCA GAAGACCCAG CCATCCCCTT GCCCCCGAGG 
CAGTTCCACC AGCTGGCTTC CCAGGGGCCC CAGGAATGCC TGGTCCGCGT CTACATTATC 
CGGGCGTTTG GCCTGCAGCC CAAGGATCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCGGT GAGTGACCAG GACAGCTACA TCCCTTGTAC TCTGGAGCCT 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC 
ACCCTCTATG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGGGAGAC GGTCATTGAC 
CTGGAGAACA GGCTGCTGTC TAAGTTCGGG GCCCGCTGTG GACTCCCCCA GACCTACTGC 
GTCTCTGGAC CCAACCAGTG GAGGGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTGTTC 
TGCCAGCAGC ACCGAGTCAA GGCCCCCGTG TACCGGACGG ACCGCGTGGT GTTTCAGGAT 
AAAGAATACA CCGTCGAAGA AATAGAGGCT GGCAGGGTCC CGAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCAGG GGCTGATCCC TGAGCACGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCGGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGGCCGGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACCAAGGA TGTGATCCTG 
GATGACCTCA GCATCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT 
GGCTTTGAAG AACACAAGCA AAAGACAGAT GTACATTATC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCGT CTTCCCTTTT GACTACCTGC CAGCTGAGCA GGTCTGCACC 
GTCTCCAAGA AGGATGCATT CTGGAGGCTG GACAAGACGG AGAGCAAGAT CCCTGCACGA 
GTGGTATTCC AGATCTGGGA CAACGACAAG TTCTCCTTCG ATGACTTTCT GGGCTCTCTG 
CAGCTGGACC TTAACCACAT GCCCAAGCCC GCCAAGACAG CTGAGAAGTG CTCTGCGGAC 
CAGCTGGAAG ACACCTTCCA CCCAGAGCGG TTTGTGTCTC TTTTTGAACA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAGATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCAGC CGGCCACGGC 
CGGGATGAAC CTAACATGAA CCCCAAGCTC GAGGACCCAA GGCGGCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGCTGT 
GCCATCATCT TCTTCCTCAT TCTCTTCATC TTCCTGCTCT TCTTGGGCAT CTTTGTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473389.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328875.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa91835
Clone ID Related Accession (Same CDS sequence)	XM_015473389.1
Accession Version	XM_015473389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT GCCAACGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGTTTGCA 
GATGGTCTCT CGGGCTTGGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCTTCATAAT 
CAGAGCAGGC TCCTGGGAGA TCCCTTAGTT GACCGCCTCA TACAGAGGAG GAAAGCAGGC 
CCCAGAATGG GCTTCCTGAC GCCTTGTCCA GTGCCCTTCC CATGGAACTG GGAGGAGGAG 
TTCATTGATT GGTGGAGCAA ATTCTTTGCT TCAATTGGGG AGAGCGAAAA GTGCGGGTCC 
TACCTAGAGA AGGACTTTGA CACTCTAAAG GTCTATGACA CATCCCTGGA GAATGTGAAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAT ACATTCAAGC TCTACCGGGG CAAGACACAG 
GAGGAGACTG AAGACCCATC AGTCATTGGG GAGTTTAAGG GACTCTTCAA AATTTATCCT 
CTCCCAGAAG ACCCAGCCAT CCCCTTGCCC CCGAGGCAGT TCCACCAGCT GGCTTCCCAG 
GGGCCCCAGG AATGCCTGGT CCGCGTCTAC ATTATCCGGG CGTTTGGCCT GCAGCCCAAG 
GATCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCGGTGAGT 
GACCAGGACA GCTACATCCC TTGTACTCTG GAGCCTGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACCC TCTATGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG GGAGACGGTC ATTGACCTGG AGAACAGGCT GCTGTCTAAG 
TTCGGGGCCC GCTGTGGACT CCCCCAGACC TACTGCGTCT CTGGACCCAA CCAGTGGAGG 
GATCAGCTCC GCCCCTCCCA GCTCCTCCAC CTGTTCTGCC AGCAGCACCG AGTCAAGGCC 
CCCGTGTACC GGACGGACCG CGTGGTGTTT CAGGATAAAG AATACACCGT CGAAGAAATA 
GAGGCTGGCA GGGTCCCGAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCCTTGCAC 
GTCCTCCAGC AGCAGGGGCT GATCCCTGAG CACGTGGAGT CCCGGCCTCT CTACAGCCCC 
CTGCAGCCGG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CGGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAACAC CAAGGATGTG ATCCTGGATG ACCTCAGCAT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGGTTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGATGTAC ATTATCGGTC CCTGGGAGGT GAAGGCAACT TTAACTGGAG GTTCGTCTTC 
CCTTTTGACT ACCTGCCAGC TGAGCAGGTC TGCACCGTCT CCAAGAAGGA TGCATTCTGG 
AGGCTGGACA AGACGGAGAG CAAGATCCCT GCACGAGTGG TATTCCAGAT CTGGGACAAC 
GACAAGTTCT CCTTCGATGA CTTTCTGGGC TCTCTGCAGC TGGACCTTAA CCACATGCCC 
AAGCCCGCCA AGACAGCTGA GAAGTGCTCT GCGGACCAGC TGGAAGACAC CTTCCACCCA 
GAGCGGTTTG TGTCTCTTTT TGAACAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTGGCG 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAGA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCAGCCGGC CACGGCCGGG ATGAACCTAA CATGAACCCC 
AAGCTCGAGG ACCCAAGGCG GCCTGACACC TCCTTCCTCT GGTTCACCTC CCCGTACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGGTTC CGCTGTGCCA TCATCTTCTT CCTCATTCTC 
TTCATCTTCC TGCTCTTCTT GGGCATCTTT GTCTACTCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328875.1
CDS	131..6694
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEELCAPAFFLQGLLPTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHNQSRLLGDPLVDRLIQRRKAGPRMGFLTPCPVPFPWNWEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473389.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473389.1

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT GCCAACGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGTTTGCA 
GATGGTCTCT CGGGCTTGGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCTTCATAAT 
CAGAGCAGGC TCCTGGGAGA TCCCTTAGTT GACCGCCTCA TACAGAGGAG GAAAGCAGGC 
CCCAGAATGG GCTTCCTGAC GCCTTGTCCA GTGCCCTTCC CATGGAACTG GGAGGAGGAG 
TTCATTGATT GGTGGAGCAA ATTCTTTGCT TCAATTGGGG AGAGCGAAAA GTGCGGGTCC 
TACCTAGAGA AGGACTTTGA CACTCTAAAG GTCTATGACA CATCCCTGGA GAATGTGAAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAT ACATTCAAGC TCTACCGGGG CAAGACACAG 
GAGGAGACTG AAGACCCATC AGTCATTGGG GAGTTTAAGG GACTCTTCAA AATTTATCCT 
CTCCCAGAAG ACCCAGCCAT CCCCTTGCCC CCGAGGCAGT TCCACCAGCT GGCTTCCCAG 
GGGCCCCAGG AATGCCTGGT CCGCGTCTAC ATTATCCGGG CGTTTGGCCT GCAGCCCAAG 
GATCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCGGTGAGT 
GACCAGGACA GCTACATCCC TTGTACTCTG GAGCCTGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACCC TCTATGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG GGAGACGGTC ATTGACCTGG AGAACAGGCT GCTGTCTAAG 
TTCGGGGCCC GCTGTGGACT CCCCCAGACC TACTGCGTCT CTGGACCCAA CCAGTGGAGG 
GATCAGCTCC GCCCCTCCCA GCTCCTCCAC CTGTTCTGCC AGCAGCACCG AGTCAAGGCC 
CCCGTGTACC GGACGGACCG CGTGGTGTTT CAGGATAAAG AATACACCGT CGAAGAAATA 
GAGGCTGGCA GGGTCCCGAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCCTTGCAC 
GTCCTCCAGC AGCAGGGGCT GATCCCTGAG CACGTGGAGT CCCGGCCTCT CTACAGCCCC 
CTGCAGCCGG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CGGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAACAC CAAGGATGTG ATCCTGGATG ACCTCAGCAT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGGTTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGATGTAC ATTATCGGTC CCTGGGAGGT GAAGGCAACT TTAACTGGAG GTTCGTCTTC 
CCTTTTGACT ACCTGCCAGC TGAGCAGGTC TGCACCGTCT CCAAGAAGGA TGCATTCTGG 
AGGCTGGACA AGACGGAGAG CAAGATCCCT GCACGAGTGG TATTCCAGAT CTGGGACAAC 
GACAAGTTCT CCTTCGATGA CTTTCTGGGC TCTCTGCAGC TGGACCTTAA CCACATGCCC 
AAGCCCGCCA AGACAGCTGA GAAGTGCTCT GCGGACCAGC TGGAAGACAC CTTCCACCCA 
GAGCGGTTTG TGTCTCTTTT TGAACAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTGGCG 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAGA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCAGCCGGC CACGGCCGGG ATGAACCTAA CATGAACCCC 
AAGCTCGAGG ACCCAAGGCG GCCTGACACC TCCTTCCTCT GGTTCACCTC CCCGTACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGGTTC CGCTGTGCCA TCATCTTCTT CCTCATTCTC 
TTCATCTTCC TGCTCTTCTT GGGCATCTTT GTCTACTCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473390.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328876.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa91836
Clone ID Related Accession (Same CDS sequence)	XM_015473390.1
Accession Version	XM_015473390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGGTG GGGGCCAGAG CCTGGCCGAG 
ACCTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAC ACTCTGGAAG GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGACGAG 
GCCGAGCCAT TCCTGGACCA GAGCGGGGCT CTGGGCCCGG GGGCCCCCAG CACCCCGAAG 
AAACAACCTT CCCATCCGCC CCCCTACCAC CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT 
GCGCCCAGCA AGCCTCTGTC GGACAAGCCG CAGGACTTCC AGATCAGGGT CCAGGTGATC 
AAGGGACGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG 
CAGACCAAGC GGACGCGGAT CCACAAAGGA AACAGCCCCG TCTTCAATGA GACTCTCTTC 
TTCAACGTGT TTGACTCTCC CGCGGAGCTG TTCAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGAAC AGATGCTCTC ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC 
ATCTACCGAG AGCCCCGACA TGCCTACCTC AGGAAGTGGC TGCTGCTCTC AGATCCCGAC 
GATTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA 
GATGAAGCCC CCCTGGAGAG AAAAGACCCC TCTGAAGACA AGGAGGACAT CGAAAGCAAC 
CTGCTTAGGC CGATCGGCGT GGCCCTCCGT GGAGCGCACT TCTGTCTGAA GGTCTTCAGG 
GCCGAGGACT TGCCACAGAT GGACGATGCC GTGATGGACA ACGTGAGGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACG GCCAATCCTC AGTGGAACCA GAGCATCACA 
CTGCCTGCCA TGTTTCCATC CATGTGTGAG AAAATGAGGA TTCGTGTCGT AGACTGGGAC 
CGCCTCACCC ACAACGATAT TGTGGCCACC ACCTACCTGA GTATGTCGAA GATCTCTGCC 
TCTGGAGGCG AGATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTCCTCC AGCCACAGAA 
CTGGACGACC ATCTAGGCTT CCTCCCCACC TTCGGGCCCT GCTACATCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAT CCCTATGCAG AGCTCAACAC GGGCAAGGGA 
GAAGGCGTGG CCTACCGAGG CCGGCTCCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCGC 
AGTGAACAGA AAGTGGAGGC TCTCTCTGCC GATGACATCC TTCGGGTAGA GAAGTACCTC 
CGGAGGCGCA AGTACTCGCT CTTTGCCGCC TTCTACTCGG CCACCATGCT GCAGGATGTC 
GATGACGCCG TCCAGTTCGA GGTCAGCATT GGGAACTATG GCAACAAGTT CGACACCACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC 
TACTACTTAC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC 
ATCCGCCACA GAGTTGAGGC TCAGAACCAG CTTCTCAGGA TTGCCGACCA GCTGGAAGCC 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCT CCGACGAGGA CGCGGACTCC 
CTGGTGGCTC AGCTGATGGA TGAGCTCATC GCAGGCTGCA GCCAACCCCT GGGTGATGTC 
CAGGAGATGC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACCAGCTGCG CACCCGCCAC 
CTGAGCCAGA TCACCGAGGC GGCCCAGGCC CTGAAGCTCG GCCACAGTGA GCTCCCCGCT 
GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG 
AACAGCCTGC CTGACATCGT CATCTGGATG CTTCAGGGGG ACAAGCGGGT GGCGTACCAG 
CGGGTGCCTG CCCATGAGGT CCTCTTCTCC CGCCGGGGCA CCAGCTACTG TGGCAAGAAC 
TGTGGAAAAC TGCAGACGAT ATTTCTGAAA TATCCGATGG AAGGGGTGCC CAGAGCCCGG 
ATGCCGGTGC AGATTCGGGT CAGGCTCTGG TTTGGACTCT CTGTGGATGA AAAGGAGTTC 
AACCAGTTTG CTGAGGGCAA GCTCTCCGTC TTCGCTGAAA CTTATGAGAA CCAGACCAAG 
CTGGCCCTGG TTGGGAACTG GGGCACAACT GGCCTCACCT ACCCCAAGTT TTCTGACATC 
ACGGGCAGGA TCAAGCTACC CAAGGACAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACA CTGCTTCACG ACACTGATGC TGGCCACCTG 
AGCTTCGTGG AGGAGGTATT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAG 
TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTGAA TCGGGCTGTC 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATCCCGCCGG ACCGGAAGCC GAGGCACTGG 
GTCCCTGCCG AGAAGATGTA CTACACACAC CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG 
AGGGACCTCA GCCAGATGGA GGCGTTGAAG CGGCACAGGC AGGCGGAGGC AGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG AAGTTCCATC TGGAGTACCG CAAGACAGAC 
GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG 
GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT GGCGTGGTGG ACGACAGGAG TGAAGATTCC 
GTGTCCGTCT CCACCTTGAG TTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT ACGCTGCTAC ATGTACCAGG CCCGGGATCT GCCTGCAATG 
GACAAGGACT CTTTTTCCGA TCCCTATGCA GTCGTCTCCT TCCTGCACCA AAGCCAGAAG 
ACGGTGGTGG CTAAGAACAC CCTAAACCCC ACCTGGGACC AGACGCTCAT CTTCTACGAG 
ATCGAGATCT TTGGCGAGCC AAGCAGCATC GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGTGTAGAT GAGTTCATGG GACGTTGCAT CTGTCAGCCG 
AGTCTGGAAC GGACGCCTCG GCTGGCCTGG TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCTGCCAT CCACCATATT 
CCTGGTTTTG AGGTACAGGA CACAACAGGG ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTGCC AACGTCAGAG GACACGGACC TGCCCTACCC ACCGCCCCAG 
AGGGAGGCCA ACATCTACAT GGTCCCCCAG AACATCAAGC CGGTTCTCCA GCGAACCGCC 
ATCGAGATCC TGGCGTGGGG TCTTCGGAAC ATGAAGAGCT ACCAGCTGGC CAGCGTGTCC 
TCCCCGAGCC TGGTGGTGGA GTGCGGGGGC CAGTCTGTAC AATCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT CGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTGT ACTGTCCCCC CATCGTGGTC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCCGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGG GCTTCCTGTG TGATCCTTAC 
TCAGAGGAGA GTCCGTCCCC ACAGGGTGGC CCAGATGACG TGAGCTTACT CAGTCCTGGG 
GAAGATGTGC TGATTGACAT CGATGACAAG GAGCCCCTCA TCCCCATCCA GTTTGCAGAT 
GGTCTCTCGG GCTTGGCCCC CACTAACATG GCATCTTCTC CATCCAGTCT TCATAATCAG 
AGCAGGCTCC TGGGAGATCC CTTAGTTGAC CGCCTCATAC AGAGGAGGAA AGCAGGCCCC 
AGAATGGGCT TCCTGACGCC TTGTCCAGTG CCCTTCCCAT GGAACTGGGA GGAGGAGTTC 
ATTGATTGGT GGAGCAAATT CTTTGCTTCA ATTGGGGAGA GCGAAAAGTG CGGGTCCTAC 
CTAGAGAAGG ACTTTGACAC TCTAAAGGTC TATGACACAT CCCTGGAGAA TGTGAAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAATACA TTCAAGCTCT ACCGGGGCAA GACACAGGAG 
GAGACTGAAG ACCCATCAGT CATTGGGGAG TTTAAGGGAC TCTTCAAAAT TTATCCTCTC 
CCAGAAGACC CAGCCATCCC CTTGCCCCCG AGGCAGTTCC ACCAGCTGGC TTCCCAGGGG 
CCCCAGGAAT GCCTGGTCCG CGTCTACATT ATCCGGGCGT TTGGCCTGCA GCCCAAGGAT 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC GGTGAGTGAC 
CAGGACAGCT ACATCCCTTG TACTCTGGAG CCTGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACCCTCT ATGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGGGA GACGGTCATT GACCTGGAGA ACAGGCTGCT GTCTAAGTTC 
GGGGCCCGCT GTGGACTCCC CCAGACCTAC TGCGTCTCTG GACCCAACCA GTGGAGGGAT 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTG TTCTGCCAGC AGCACCGAGT CAAGGCCCCC 
GTGTACCGGA CGGACCGCGT GGTGTTTCAG GATAAAGAAT ACACCGTCGA AGAAATAGAG 
GCTGGCAGGG TCCCGAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC CTTGCACGTC 
CTCCAGCAGC AGGGGCTGAT CCCTGAGCAC GTGGAGTCCC GGCCTCTCTA CAGCCCCCTG 
CAGCCGGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGGCCGG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAACACCAA GGATGTGATC CTGGATGACC TCAGCATCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG GTTGGCTTTG AAGAACACAA GCAAAAGACA 
GATGTACATT ATCGGTCCCT GGGAGGTGAA GGCAACTTTA ACTGGAGGTT CGTCTTCCCT 
TTTGACTACC TGCCAGCTGA GCAGGTCTGC ACCGTCTCCA AGAAGGATGC ATTCTGGAGG 
CTGGACAAGA CGGAGAGCAA GATCCCTGCA CGAGTGGTAT TCCAGATCTG GGACAACGAC 
AAGTTCTCCT TCGATGACTT TCTGGGCTCT CTGCAGCTGG ACCTTAACCA CATGCCCAAG 
CCCGCCAAGA CAGCTGAGAA GTGCTCTGCG GACCAGCTGG AAGACACCTT CCACCCAGAG 
CGGTTTGTGT CTCTTTTTGA ACAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTGGCGGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAGATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC AGCCGGCCAC GGCCGGGATG AACCTAACAT GAACCCCAAG 
CTCGAGGACC CAAGGCGGCC TGACACCTCC TTCCTCTGGT TCACCTCCCC GTACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGGTTCCGC TGTGCCATCA TCTTCTTCCT CATTCTCTTC 
ATCTTCCTGC TCTTCTTGGG CATCTTTGTC TACTCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328876.1
CDS	546..7106
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEELCAPAFFLQGLLPTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHNQSRLLGDPLVDRLIQRRKAGPRMGFLTPCPVPFPWNWEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473390.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473390.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGGTG GGGGCCAGAG CCTGGCCGAG 
ACCTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAC ACTCTGGAAG GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGACGAG 
GCCGAGCCAT TCCTGGACCA GAGCGGGGCT CTGGGCCCGG GGGCCCCCAG CACCCCGAAG 
AAACAACCTT CCCATCCGCC CCCCTACCAC CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT 
GCGCCCAGCA AGCCTCTGTC GGACAAGCCG CAGGACTTCC AGATCAGGGT CCAGGTGATC 
AAGGGACGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG 
CAGACCAAGC GGACGCGGAT CCACAAAGGA AACAGCCCCG TCTTCAATGA GACTCTCTTC 
TTCAACGTGT TTGACTCTCC CGCGGAGCTG TTCAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGAAC AGATGCTCTC ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC 
ATCTACCGAG AGCCCCGACA TGCCTACCTC AGGAAGTGGC TGCTGCTCTC AGATCCCGAC 
GATTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA 
GATGAAGCCC CCCTGGAGAG AAAAGACCCC TCTGAAGACA AGGAGGACAT CGAAAGCAAC 
CTGCTTAGGC CGATCGGCGT GGCCCTCCGT GGAGCGCACT TCTGTCTGAA GGTCTTCAGG 
GCCGAGGACT TGCCACAGAT GGACGATGCC GTGATGGACA ACGTGAGGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACG GCCAATCCTC AGTGGAACCA GAGCATCACA 
CTGCCTGCCA TGTTTCCATC CATGTGTGAG AAAATGAGGA TTCGTGTCGT AGACTGGGAC 
CGCCTCACCC ACAACGATAT TGTGGCCACC ACCTACCTGA GTATGTCGAA GATCTCTGCC 
TCTGGAGGCG AGATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTCCTCC AGCCACAGAA 
CTGGACGACC ATCTAGGCTT CCTCCCCACC TTCGGGCCCT GCTACATCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAT CCCTATGCAG AGCTCAACAC GGGCAAGGGA 
GAAGGCGTGG CCTACCGAGG CCGGCTCCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCGC 
AGTGAACAGA AAGTGGAGGC TCTCTCTGCC GATGACATCC TTCGGGTAGA GAAGTACCTC 
CGGAGGCGCA AGTACTCGCT CTTTGCCGCC TTCTACTCGG CCACCATGCT GCAGGATGTC 
GATGACGCCG TCCAGTTCGA GGTCAGCATT GGGAACTATG GCAACAAGTT CGACACCACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC 
TACTACTTAC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC 
ATCCGCCACA GAGTTGAGGC TCAGAACCAG CTTCTCAGGA TTGCCGACCA GCTGGAAGCC 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCT CCGACGAGGA CGCGGACTCC 
CTGGTGGCTC AGCTGATGGA TGAGCTCATC GCAGGCTGCA GCCAACCCCT GGGTGATGTC 
CAGGAGATGC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACCAGCTGCG CACCCGCCAC 
CTGAGCCAGA TCACCGAGGC GGCCCAGGCC CTGAAGCTCG GCCACAGTGA GCTCCCCGCT 
GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG 
AACAGCCTGC CTGACATCGT CATCTGGATG CTTCAGGGGG ACAAGCGGGT GGCGTACCAG 
CGGGTGCCTG CCCATGAGGT CCTCTTCTCC CGCCGGGGCA CCAGCTACTG TGGCAAGAAC 
TGTGGAAAAC TGCAGACGAT ATTTCTGAAA TATCCGATGG AAGGGGTGCC CAGAGCCCGG 
ATGCCGGTGC AGATTCGGGT CAGGCTCTGG TTTGGACTCT CTGTGGATGA AAAGGAGTTC 
AACCAGTTTG CTGAGGGCAA GCTCTCCGTC TTCGCTGAAA CTTATGAGAA CCAGACCAAG 
CTGGCCCTGG TTGGGAACTG GGGCACAACT GGCCTCACCT ACCCCAAGTT TTCTGACATC 
ACGGGCAGGA TCAAGCTACC CAAGGACAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACA CTGCTTCACG ACACTGATGC TGGCCACCTG 
AGCTTCGTGG AGGAGGTATT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAG 
TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTGAA TCGGGCTGTC 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATCCCGCCGG ACCGGAAGCC GAGGCACTGG 
GTCCCTGCCG AGAAGATGTA CTACACACAC CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG 
AGGGACCTCA GCCAGATGGA GGCGTTGAAG CGGCACAGGC AGGCGGAGGC AGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG AAGTTCCATC TGGAGTACCG CAAGACAGAC 
GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG 
GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT GGCGTGGTGG ACGACAGGAG TGAAGATTCC 
GTGTCCGTCT CCACCTTGAG TTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT ACGCTGCTAC ATGTACCAGG CCCGGGATCT GCCTGCAATG 
GACAAGGACT CTTTTTCCGA TCCCTATGCA GTCGTCTCCT TCCTGCACCA AAGCCAGAAG 
ACGGTGGTGG CTAAGAACAC CCTAAACCCC ACCTGGGACC AGACGCTCAT CTTCTACGAG 
ATCGAGATCT TTGGCGAGCC AAGCAGCATC GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGTGTAGAT GAGTTCATGG GACGTTGCAT CTGTCAGCCG 
AGTCTGGAAC GGACGCCTCG GCTGGCCTGG TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCTGCCAT CCACCATATT 
CCTGGTTTTG AGGTACAGGA CACAACAGGG ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTGCC AACGTCAGAG GACACGGACC TGCCCTACCC ACCGCCCCAG 
AGGGAGGCCA ACATCTACAT GGTCCCCCAG AACATCAAGC CGGTTCTCCA GCGAACCGCC 
ATCGAGATCC TGGCGTGGGG TCTTCGGAAC ATGAAGAGCT ACCAGCTGGC CAGCGTGTCC 
TCCCCGAGCC TGGTGGTGGA GTGCGGGGGC CAGTCTGTAC AATCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT CGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTGT ACTGTCCCCC CATCGTGGTC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCCGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGG GCTTCCTGTG TGATCCTTAC 
TCAGAGGAGA GTCCGTCCCC ACAGGGTGGC CCAGATGACG TGAGCTTACT CAGTCCTGGG 
GAAGATGTGC TGATTGACAT CGATGACAAG GAGCCCCTCA TCCCCATCCA GTTTGCAGAT 
GGTCTCTCGG GCTTGGCCCC CACTAACATG GCATCTTCTC CATCCAGTCT TCATAATCAG 
AGCAGGCTCC TGGGAGATCC CTTAGTTGAC CGCCTCATAC AGAGGAGGAA AGCAGGCCCC 
AGAATGGGCT TCCTGACGCC TTGTCCAGTG CCCTTCCCAT GGAACTGGGA GGAGGAGTTC 
ATTGATTGGT GGAGCAAATT CTTTGCTTCA ATTGGGGAGA GCGAAAAGTG CGGGTCCTAC 
CTAGAGAAGG ACTTTGACAC TCTAAAGGTC TATGACACAT CCCTGGAGAA TGTGAAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAATACA TTCAAGCTCT ACCGGGGCAA GACACAGGAG 
GAGACTGAAG ACCCATCAGT CATTGGGGAG TTTAAGGGAC TCTTCAAAAT TTATCCTCTC 
CCAGAAGACC CAGCCATCCC CTTGCCCCCG AGGCAGTTCC ACCAGCTGGC TTCCCAGGGG 
CCCCAGGAAT GCCTGGTCCG CGTCTACATT ATCCGGGCGT TTGGCCTGCA GCCCAAGGAT 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC GGTGAGTGAC 
CAGGACAGCT ACATCCCTTG TACTCTGGAG CCTGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACCCTCT ATGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGGGA GACGGTCATT GACCTGGAGA ACAGGCTGCT GTCTAAGTTC 
GGGGCCCGCT GTGGACTCCC CCAGACCTAC TGCGTCTCTG GACCCAACCA GTGGAGGGAT 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTG TTCTGCCAGC AGCACCGAGT CAAGGCCCCC 
GTGTACCGGA CGGACCGCGT GGTGTTTCAG GATAAAGAAT ACACCGTCGA AGAAATAGAG 
GCTGGCAGGG TCCCGAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC CTTGCACGTC 
CTCCAGCAGC AGGGGCTGAT CCCTGAGCAC GTGGAGTCCC GGCCTCTCTA CAGCCCCCTG 
CAGCCGGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGGCCGG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAACACCAA GGATGTGATC CTGGATGACC TCAGCATCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG GTTGGCTTTG AAGAACACAA GCAAAAGACA 
GATGTACATT ATCGGTCCCT GGGAGGTGAA GGCAACTTTA ACTGGAGGTT CGTCTTCCCT 
TTTGACTACC TGCCAGCTGA GCAGGTCTGC ACCGTCTCCA AGAAGGATGC ATTCTGGAGG 
CTGGACAAGA CGGAGAGCAA GATCCCTGCA CGAGTGGTAT TCCAGATCTG GGACAACGAC 
AAGTTCTCCT TCGATGACTT TCTGGGCTCT CTGCAGCTGG ACCTTAACCA CATGCCCAAG 
CCCGCCAAGA CAGCTGAGAA GTGCTCTGCG GACCAGCTGG AAGACACCTT CCACCCAGAG 
CGGTTTGTGT CTCTTTTTGA ACAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTGGCGGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAGATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC AGCCGGCCAC GGCCGGGATG AACCTAACAT GAACCCCAAG 
CTCGAGGACC CAAGGCGGCC TGACACCTCC TTCCTCTGGT TCACCTCCCC GTACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGGTTCCGC TGTGCCATCA TCTTCTTCCT CATTCTCTTC 
ATCTTCCTGC TCTTCTTGGG CATCTTTGTC TACTCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473391.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328877.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa91837
Clone ID Related Accession (Same CDS sequence)	XM_015473391.1
Accession Version	XM_015473391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGTCAGA GGACACGGAC 
CTGCCCTACC CACCGCCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACCGC CATCGAGATC CTGGCGTGGG GTCTTCGGAA CATGAAGAGC 
TACCAGCTGG CCAGCGTGTC CTCCCCGAGC CTGGTGGTGG AGTGCGGGGG CCAGTCTGTA 
CAATCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTG TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCCGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
GGCTTCCTGT GTGATCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATCC AGTTTGCAGA TGGTCTCTCG GGCTTGGCCC CCACTAACAT GGCATCTTCT 
CCATCCAGTC TTCATAATCA GAGCAGGCTC CTGGGAGATC CCTTAGTTGA CCGCCTCATA 
CAGAGGAGGA AAGCAGGCCC CAGAATGGGC TTCCTGACGC CTTGTCCAGT GCCCTTCCCA 
TGGAACTGGG AGGAGGAGTT CATTGATTGG TGGAGCAAAT TCTTTGCTTC AATTGGGGAG 
AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG GACTTTGACA CTCTAAAGGT CTATGACACA 
TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC CTGTCTGACT TTTGTAATAC ATTCAAGCTC 
TACCGGGGCA AGACACAGGA GGAGACTGAA GACCCATCAG TCATTGGGGA GTTTAAGGGA 
CTCTTCAAAA TTTATCCTCT CCCAGAAGAC CCAGCCATCC CCTTGCCCCC GAGGCAGTTC 
CACCAGCTGG CTTCCCAGGG GCCCCAGGAA TGCCTGGTCC GCGTCTACAT TATCCGGGCG 
TTTGGCCTGC AGCCCAAGGA TCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CGGTGAGTGA CCAGGACAGC TACATCCCTT GTACTCTGGA GCCTGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACCCTC 
TATGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGGG AGACGGTCAT TGACCTGGAG 
AACAGGCTGC TGTCTAAGTT CGGGGCCCGC TGTGGACTCC CCCAGACCTA CTGCGTCTCT 
GGACCCAACC AGTGGAGGGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT GTTCTGCCAG 
CAGCACCGAG TCAAGGCCCC CGTGTACCGG ACGGACCGCG TGGTGTTTCA GGATAAAGAA 
TACACCGTCG AAGAAATAGA GGCTGGCAGG GTCCCGAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CCTTGCACGT CCTCCAGCAG CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC 
CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCG GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAACACCA AGGATGTGAT CCTGGATGAC 
CTCAGCATCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTACAT TATCGGTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCGTCTTCCC TTTTGACTAC CTGCCAGCTG AGCAGGTCTG CACCGTCTCC 
AAGAAGGATG CATTCTGGAG GCTGGACAAG ACGGAGAGCA AGATCCCTGC ACGAGTGGTA 
TTCCAGATCT GGGACAACGA CAAGTTCTCC TTCGATGACT TTCTGGGCTC TCTGCAGCTG 
GACCTTAACC ACATGCCCAA GCCCGCCAAG ACAGCTGAGA AGTGCTCTGC GGACCAGCTG 
GAAGACACCT TCCACCCAGA GCGGTTTGTG TCTCTTTTTG AACAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAGATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT 
GAACCTAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGGC CTGACACCTC CTTCCTCTGG 
TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCCTGTGGC GGCGGTTCCG CTGTGCCATC 
ATCTTCTTCC TCATTCTCTT CATCTTCCTG CTCTTCTTGG GCATCTTTGT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328877.1
CDS	131..6652
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHNQSRLLGDPLVDRLIQRRKAGPRMGFLTPCPVPFPWNWEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473391.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473391.1

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGTCAGA GGACACGGAC 
CTGCCCTACC CACCGCCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACCGC CATCGAGATC CTGGCGTGGG GTCTTCGGAA CATGAAGAGC 
TACCAGCTGG CCAGCGTGTC CTCCCCGAGC CTGGTGGTGG AGTGCGGGGG CCAGTCTGTA 
CAATCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTG TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCCGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
GGCTTCCTGT GTGATCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATCC AGTTTGCAGA TGGTCTCTCG GGCTTGGCCC CCACTAACAT GGCATCTTCT 
CCATCCAGTC TTCATAATCA GAGCAGGCTC CTGGGAGATC CCTTAGTTGA CCGCCTCATA 
CAGAGGAGGA AAGCAGGCCC CAGAATGGGC TTCCTGACGC CTTGTCCAGT GCCCTTCCCA 
TGGAACTGGG AGGAGGAGTT CATTGATTGG TGGAGCAAAT TCTTTGCTTC AATTGGGGAG 
AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG GACTTTGACA CTCTAAAGGT CTATGACACA 
TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC CTGTCTGACT TTTGTAATAC ATTCAAGCTC 
TACCGGGGCA AGACACAGGA GGAGACTGAA GACCCATCAG TCATTGGGGA GTTTAAGGGA 
CTCTTCAAAA TTTATCCTCT CCCAGAAGAC CCAGCCATCC CCTTGCCCCC GAGGCAGTTC 
CACCAGCTGG CTTCCCAGGG GCCCCAGGAA TGCCTGGTCC GCGTCTACAT TATCCGGGCG 
TTTGGCCTGC AGCCCAAGGA TCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CGGTGAGTGA CCAGGACAGC TACATCCCTT GTACTCTGGA GCCTGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACCCTC 
TATGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGGG AGACGGTCAT TGACCTGGAG 
AACAGGCTGC TGTCTAAGTT CGGGGCCCGC TGTGGACTCC CCCAGACCTA CTGCGTCTCT 
GGACCCAACC AGTGGAGGGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT GTTCTGCCAG 
CAGCACCGAG TCAAGGCCCC CGTGTACCGG ACGGACCGCG TGGTGTTTCA GGATAAAGAA 
TACACCGTCG AAGAAATAGA GGCTGGCAGG GTCCCGAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CCTTGCACGT CCTCCAGCAG CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC 
CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCG GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAACACCA AGGATGTGAT CCTGGATGAC 
CTCAGCATCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTACAT TATCGGTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCGTCTTCCC TTTTGACTAC CTGCCAGCTG AGCAGGTCTG CACCGTCTCC 
AAGAAGGATG CATTCTGGAG GCTGGACAAG ACGGAGAGCA AGATCCCTGC ACGAGTGGTA 
TTCCAGATCT GGGACAACGA CAAGTTCTCC TTCGATGACT TTCTGGGCTC TCTGCAGCTG 
GACCTTAACC ACATGCCCAA GCCCGCCAAG ACAGCTGAGA AGTGCTCTGC GGACCAGCTG 
GAAGACACCT TCCACCCAGA GCGGTTTGTG TCTCTTTTTG AACAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAGATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT 
GAACCTAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGGC CTGACACCTC CTTCCTCTGG 
TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCCTGTGGC GGCGGTTCCG CTGTGCCATC 
ATCTTCTTCC TCATTCTCTT CATCTTCCTG CTCTTCTTGG GCATCTTTGT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473392.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328878.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa91838
Clone ID Related Accession (Same CDS sequence)	XM_015473392.1
Accession Version	XM_015473392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGTGGACGAC CATCTAGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACATCA ACCTCTATGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA TCCCTATGCA 
GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTCCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCG CAGTGAACAG AAAGTGGAGG CTCTCTCTGC CGATGACATC 
CTTCGGGTAG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT CGATGACGCC GTCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACCAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGATG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCGTCTT ACTGGGAGGA CATCCGCCAC AGAGTTGAGG CTCAGAACCA GCTTCTCAGG 
ATTGCCGACC AGCTGGAAGC CGGCCTGGAG CAAGTCCACT TGGCCCTGAA GGCTCAGTGC 
TCCGACGAGG ACGCGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAACCCC TGGGTGATGT CCAGGAGATG CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGCCA CCTGAGCCAG ATCACCGAGG CGGCCCAGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCTG ACGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTTCAGGGG 
GACAAGCGGG TGGCGTACCA GCGGGTGCCT GCCCATGAGG TCCTCTTCTC CCGCCGGGGC 
ACCAGCTACT GTGGCAAGAA CTGTGGAAAA CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAAGGGGTGC CCAGAGCCCG GATGCCGGTG CAGATTCGGG TCAGGCTCTG GTTTGGACTC 
TCTGTGGATG AAAAGGAGTT CAACCAGTTT GCTGAGGGCA AGCTCTCCGT CTTCGCTGAA 
ACTTATGAGA ACCAGACCAA GCTGGCCCTG GTTGGGAACT GGGGCACAAC TGGCCTCACC 
TACCCCAAGT TTTCTGACAT CACGGGCAGG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC TGGAGACTGG TTCGTGTGTC CAGAGAAGAC ACTGCTTCAC 
GACACTGATG CTGGCCACCT GAGCTTCGTG GAGGAGGTAT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTGA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCGCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGGCGGCGG 
CGCTGGGTCC GTCTGCGCCG GAGGGACCTC AGCCAGATGG AGGCGTTGAA GCGGCACAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAT 
CTGGAGTACC GCAAGACAGA CGCCTTTCGT CGTCGCCGCT GGCGCCGCCG CATGGAGCCC 
TTGGAGAAGA CAGGGCCTGC GGCTGTGTTT GCCCTTGAGG GGGCCCTGGG TGGCGTGGTG 
GACGACAGGA GTGAAGATTC CGTGTCCGTC TCCACCTTGA GTTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGATC TGCCTGCAAT GGACAAGGAC TCTTTTTCCG ATCCCTATGC AGTCGTCTCC 
TTCCTGCACC AAAGCCAGAA GACGGTGGTG GCTAAGAACA CCCTAAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CAAGCAGCAT CGCCGAGCAG 
CCTCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ATGGTGTAGA TGAGTTCATG 
GGACGTTGCA TCTGTCAGCC GAGTCTGGAA CGGACGCCTC GGCTGGCCTG GTTCCCGCTG 
ACGAGGGGCA GCCAGCCCGC CGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCTGCCA TCCACCATAT TCCTGGTTTT GAGGTACAGG ACACAACAGG GATCCTGGAA 
GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTGC CAACGTCAGA GGACACGGAC 
CTGCCCTACC CACCGCCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACCGC CATCGAGATC CTGGCGTGGG GTCTTCGGAA CATGAAGAGC 
TACCAGCTGG CCAGCGTGTC CTCCCCGAGC CTGGTGGTGG AGTGCGGGGG CCAGTCTGTA 
CAATCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTG TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCCGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
GGCTTCCTGT GTGATCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATCC AGTTTGCAGA TGGTCTCTCG GGCTTGGCCC CCACTAACAT GGCATCTTCT 
CCATCCAGTC TTCATAATCA GAGCAGGCTC CTGGGAGATC CCTTAGTTGA CCGCCTCATA 
CAGAGGAGGA AAGCAGGCCC CAGAATGGGC TTCCTGACGC CTTGTCCAGT GCCCTTCCCA 
TGGAACTGGG AGGAGGAGTT CATTGATTGG TGGAGCAAAT TCTTTGCTTC AATTGGGGAG 
AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG GACTTTGACA CTCTAAAGGT CTATGACACA 
TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC CTGTCTGACT TTTGTAATAC ATTCAAGCTC 
TACCGGGGCA AGACACAGGA GGAGACTGAA GACCCATCAG TCATTGGGGA GTTTAAGGGA 
CTCTTCAAAA TTTATCCTCT CCCAGAAGAC CCAGCCATCC CCTTGCCCCC GAGGCAGTTC 
CACCAGCTGG CTTCCCAGGG GCCCCAGGAA TGCCTGGTCC GCGTCTACAT TATCCGGGCG 
TTTGGCCTGC AGCCCAAGGA TCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CGGTGAGTGA CCAGGACAGC TACATCCCTT GTACTCTGGA GCCTGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACCCTC 
TATGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGGG AGACGGTCAT TGACCTGGAG 
AACAGGCTGC TGTCTAAGTT CGGGGCCCGC TGTGGACTCC CCCAGACCTA CTGCGTCTCT 
GGACCCAACC AGTGGAGGGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT GTTCTGCCAG 
CAGCACCGAG TCAAGGCCCC CGTGTACCGG ACGGACCGCG TGGTGTTTCA GGATAAAGAA 
TACACCGTCG AAGAAATAGA GGCTGGCAGG GTCCCGAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CCTTGCACGT CCTCCAGCAG CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC 
CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCG GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAACACCA AGGATGTGAT CCTGGATGAC 
CTCAGCATCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTACAT TATCGGTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCGTCTTCCC TTTTGACTAC CTGCCAGCTG AGCAGGTCTG CACCGTCTCC 
AAGAAGGATG CATTCTGGAG GCTGGACAAG ACGGAGAGCA AGATCCCTGC ACGAGTGGTA 
TTCCAGATCT GGGACAACGA CAAGTTCTCC TTCGATGACT TTCTGGGCTC TCTGCAGCTG 
GACCTTAACC ACATGCCCAA GCCCGCCAAG ACAGCTGAGA AGTGCTCTGC GGACCAGCTG 
GAAGACACCT TCCACCCAGA GCGGTTTGTG TCTCTTTTTG AACAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAGATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT 
GAACCTAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGGC CTGACACCTC CTTCCTCTGG 
TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCCTGTGGC GGCGGTTCCG CTGTGCCATC 
ATCTTCTTCC TCATTCTCTT CATCTTCCTG CTCTTCTTGG GCATCTTTGT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328878.1
CDS	131..6652
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEELCAPAFFLQGLLPTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHNQSRLLGDPLVDRLIQRRKAGPRMGFLTPCPVPFPWNWEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473392.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473392.1

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGTGGACGAC CATCTAGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACATCA ACCTCTATGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA TCCCTATGCA 
GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTCCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCG CAGTGAACAG AAAGTGGAGG CTCTCTCTGC CGATGACATC 
CTTCGGGTAG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT CGATGACGCC GTCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACCAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGATG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCGTCTT ACTGGGAGGA CATCCGCCAC AGAGTTGAGG CTCAGAACCA GCTTCTCAGG 
ATTGCCGACC AGCTGGAAGC CGGCCTGGAG CAAGTCCACT TGGCCCTGAA GGCTCAGTGC 
TCCGACGAGG ACGCGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAACCCC TGGGTGATGT CCAGGAGATG CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGCCA CCTGAGCCAG ATCACCGAGG CGGCCCAGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCTG ACGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTTCAGGGG 
GACAAGCGGG TGGCGTACCA GCGGGTGCCT GCCCATGAGG TCCTCTTCTC CCGCCGGGGC 
ACCAGCTACT GTGGCAAGAA CTGTGGAAAA CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAAGGGGTGC CCAGAGCCCG GATGCCGGTG CAGATTCGGG TCAGGCTCTG GTTTGGACTC 
TCTGTGGATG AAAAGGAGTT CAACCAGTTT GCTGAGGGCA AGCTCTCCGT CTTCGCTGAA 
ACTTATGAGA ACCAGACCAA GCTGGCCCTG GTTGGGAACT GGGGCACAAC TGGCCTCACC 
TACCCCAAGT TTTCTGACAT CACGGGCAGG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC TGGAGACTGG TTCGTGTGTC CAGAGAAGAC ACTGCTTCAC 
GACACTGATG CTGGCCACCT GAGCTTCGTG GAGGAGGTAT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTGA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCGCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGGCGGCGG 
CGCTGGGTCC GTCTGCGCCG GAGGGACCTC AGCCAGATGG AGGCGTTGAA GCGGCACAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAT 
CTGGAGTACC GCAAGACAGA CGCCTTTCGT CGTCGCCGCT GGCGCCGCCG CATGGAGCCC 
TTGGAGAAGA CAGGGCCTGC GGCTGTGTTT GCCCTTGAGG GGGCCCTGGG TGGCGTGGTG 
GACGACAGGA GTGAAGATTC CGTGTCCGTC TCCACCTTGA GTTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGATC TGCCTGCAAT GGACAAGGAC TCTTTTTCCG ATCCCTATGC AGTCGTCTCC 
TTCCTGCACC AAAGCCAGAA GACGGTGGTG GCTAAGAACA CCCTAAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CAAGCAGCAT CGCCGAGCAG 
CCTCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ATGGTGTAGA TGAGTTCATG 
GGACGTTGCA TCTGTCAGCC GAGTCTGGAA CGGACGCCTC GGCTGGCCTG GTTCCCGCTG 
ACGAGGGGCA GCCAGCCCGC CGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCTGCCA TCCACCATAT TCCTGGTTTT GAGGTACAGG ACACAACAGG GATCCTGGAA 
GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTGC CAACGTCAGA GGACACGGAC 
CTGCCCTACC CACCGCCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACCGC CATCGAGATC CTGGCGTGGG GTCTTCGGAA CATGAAGAGC 
TACCAGCTGG CCAGCGTGTC CTCCCCGAGC CTGGTGGTGG AGTGCGGGGG CCAGTCTGTA 
CAATCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTG TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCCGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
GGCTTCCTGT GTGATCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATCC AGTTTGCAGA TGGTCTCTCG GGCTTGGCCC CCACTAACAT GGCATCTTCT 
CCATCCAGTC TTCATAATCA GAGCAGGCTC CTGGGAGATC CCTTAGTTGA CCGCCTCATA 
CAGAGGAGGA AAGCAGGCCC CAGAATGGGC TTCCTGACGC CTTGTCCAGT GCCCTTCCCA 
TGGAACTGGG AGGAGGAGTT CATTGATTGG TGGAGCAAAT TCTTTGCTTC AATTGGGGAG 
AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG GACTTTGACA CTCTAAAGGT CTATGACACA 
TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC CTGTCTGACT TTTGTAATAC ATTCAAGCTC 
TACCGGGGCA AGACACAGGA GGAGACTGAA GACCCATCAG TCATTGGGGA GTTTAAGGGA 
CTCTTCAAAA TTTATCCTCT CCCAGAAGAC CCAGCCATCC CCTTGCCCCC GAGGCAGTTC 
CACCAGCTGG CTTCCCAGGG GCCCCAGGAA TGCCTGGTCC GCGTCTACAT TATCCGGGCG 
TTTGGCCTGC AGCCCAAGGA TCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CGGTGAGTGA CCAGGACAGC TACATCCCTT GTACTCTGGA GCCTGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACCCTC 
TATGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGGG AGACGGTCAT TGACCTGGAG 
AACAGGCTGC TGTCTAAGTT CGGGGCCCGC TGTGGACTCC CCCAGACCTA CTGCGTCTCT 
GGACCCAACC AGTGGAGGGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT GTTCTGCCAG 
CAGCACCGAG TCAAGGCCCC CGTGTACCGG ACGGACCGCG TGGTGTTTCA GGATAAAGAA 
TACACCGTCG AAGAAATAGA GGCTGGCAGG GTCCCGAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CCTTGCACGT CCTCCAGCAG CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC 
CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCG GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAACACCA AGGATGTGAT CCTGGATGAC 
CTCAGCATCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTACAT TATCGGTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCGTCTTCCC TTTTGACTAC CTGCCAGCTG AGCAGGTCTG CACCGTCTCC 
AAGAAGGATG CATTCTGGAG GCTGGACAAG ACGGAGAGCA AGATCCCTGC ACGAGTGGTA 
TTCCAGATCT GGGACAACGA CAAGTTCTCC TTCGATGACT TTCTGGGCTC TCTGCAGCTG 
GACCTTAACC ACATGCCCAA GCCCGCCAAG ACAGCTGAGA AGTGCTCTGC GGACCAGCTG 
GAAGACACCT TCCACCCAGA GCGGTTTGTG TCTCTTTTTG AACAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAGATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT 
GAACCTAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGGC CTGACACCTC CTTCCTCTGG 
TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCCTGTGGC GGCGGTTCCG CTGTGCCATC 
ATCTTCTTCC TCATTCTCTT CATCTTCCTG CTCTTCTTGG GCATCTTTGT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473394.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328880.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa91839
Clone ID Related Accession (Same CDS sequence)	XM_015473394.1
Accession Version	XM_015473394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGT GTTTCCCAGG ACAGCCTGTG CCCCAGGGAG CAGGGAAGAG AAAGCTCTCT 
GAGTCCTGCT GCAGCCCTGA GGTGCCTGGC ACGGCCCTCT CTCCCCACCC ACTGCCGGAG 
ACGGGCGAGC AGAGCAGCCA CTGGCGGGAG CGCTCCAGGA GGGCTTCATG CAAGAGGTGG 
GACTCGAGGT TCCTGGGGGA AGCCAACATC CCCCTCCGGG AAGTCCTCGC CACCCCCAGC 
CTGTCTGCCA GCTTCAATGC CCCACTGCTG GACACCAAGA AGCAGCCCAC AGGGGCCTCG 
CTGGTCCTGC AGGTGTCCTA CACGCCGCTG CCTGGAGCCG TGCCCACCTT CCCACCCTTA 
ACACCTCTGG AACCCTCCCC GACTCTGCCT GACATGGATA CGGTGGCTGG TGGGGGCCAG 
AGCCTGGCCG AGACCTGGTC CCTGCTCAGT GACAGCACCG TGGACACGAG ACACTCTGGA 
AGGAAGTGGC TGGCCCCCAC GGACACAGGA GGAGAGGAGG ACACTGAGGA CCAGGGGCTC 
ACGGGAGACG AGGCCGAGCC ATTCCTGGAC CAGAGCGGGG CTCTGGGCCC GGGGGCCCCC 
AGCACCCCGA AGAAACAACC TTCCCATCCG CCCCCCTACC ACCCCGGAGG GGGAAGGAAG 
AGAAGCGCGC CTGCGCCCAG CAAGCCTCTG TCGGACAAGC CGCAGGACTT CCAGATCAGG 
GTCCAGGTGA TCAAGGGACG CCAGCTGCCA GGGGTGAACA TCAAGCCTGT GGTCAAGGTC 
ACCGCCGCTG GGCAGACCAA GCGGACGCGG ATCCACAAAG GAAACAGCCC CGTCTTCAAT 
GAGACTCTCT TCTTCAACGT GTTTGACTCT CCCGCGGAGC TGTTCAATGA GCCCATCTTC 
ATCACGGTGG TGGACTCCCG TTCCCTCAGA ACAGATGCTC TCATCGGGGA GTTCCGGCTG 
GACGTGGGCA GCATCTACCG AGAGCCCCGA CATGCCTACC TCAGGAAGTG GCTGCTGCTC 
TCAGATCCCG ACGATTTCTC TGCTGGGGCC AGAGGCTACC TGAAAGCGAG TCTCTGTGTG 
CTGGGGCCTG GAGATGAAGC CCCCCTGGAG AGAAAAGACC CCTCTGAAGA CAAGGAGGAC 
ATCGAAAGCA ACCTGCTTAG GCCGATCGGC GTGGCCCTCC GTGGAGCGCA CTTCTGTCTG 
AAGGTCTTCA GGGCCGAGGA CTTGCCACAG ATGGACGATG CCGTGATGGA CAACGTGAGG 
CAGATCTTCG GCTTTGACAG TAACAAGAAG AACTTGGTGG ATCCCTTTGT GGAAGTCAGC 
TTTGCAGGGA AAATGCTCTG CAGCAAGATC CTGGAGAAGA CGGCCAATCC TCAGTGGAAC 
CAGAGCATCA CACTGCCTGC CATGTTTCCA TCCATGTGTG AGAAAATGAG GATTCGTGTC 
GTAGACTGGG ACCGCCTCAC CCACAACGAT ATTGTGGCCA CCACCTACCT GAGTATGTCG 
AAGATCTCTG CCTCTGGAGG CGAGATAGAA GAGGAGCCTG CAGGTGTGGT CAAGCCTCCT 
CCAGCCACAG AACTGGACGA CCATCTAGGC TTCCTCCCCA CCTTCGGGCC CTGCTACATC 
AACCTCTATG GCAGCCCCAG GGAGTTCACT GGCTTCCCGG ATCCCTATGC AGAGCTCAAC 
ACGGGCAAGG GAGAAGGCGT GGCCTACCGA GGCCGGCTCC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC GCAGTGAACA GAAAGTGGAG GCTCTCTCTG CCGATGACAT CCTTCGGGTA 
GAGAAGTACC TCCGGAGGCG CAAGTACTCG CTCTTTGCCG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TCGATGACGC CGTCCAGTTC GAGGTCAGCA TTGGGAACTA TGGCAACAAG 
TTCGACACCA CCTGCCTGCC GCTGGCCTCC ACCACCCAGT ACAGCCGGGC GGTCTTTGAT 
GGATGCCACT ACTACTACTT ACCCTGGGGC AACGTGAAGC CCGTGGTGGT GCTGTCGTCT 
TACTGGGAGG ACATCCGCCA CAGAGTTGAG GCTCAGAACC AGCTTCTCAG GATTGCCGAC 
CAGCTGGAAG CCGGCCTGGA GCAAGTCCAC TTGGCCCTGA AGGCTCAGTG CTCCGACGAG 
GACGCGGACT CCCTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAACCC 
CTGGGTGATG TCCAGGAGAT GCCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGCC ACCTGAGCCA GATCACCGAG GCGGCCCAGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCCG CTGCCCTGGA GCAGGCTGAG GACTGGCTCC TGCGGCTCCG TGCCCTGGCT 
GACGAGCCCC AGAACAGCCT GCCTGACATC GTCATCTGGA TGCTTCAGGG GGACAAGCGG 
GTGGCGTACC AGCGGGTGCC TGCCCATGAG GTCCTCTTCT CCCGCCGGGG CACCAGCTAC 
TGTGGCAAGA ACTGTGGAAA ACTGCAGACG ATATTTCTGA AATATCCGAT GGAAGGGGTG 
CCCAGAGCCC GGATGCCGGT GCAGATTCGG GTCAGGCTCT GGTTTGGACT CTCTGTGGAT 
GAAAAGGAGT TCAACCAGTT TGCTGAGGGC AAGCTCTCCG TCTTCGCTGA AACTTATGAG 
AACCAGACCA AGCTGGCCCT GGTTGGGAAC TGGGGCACAA CTGGCCTCAC CTACCCCAAG 
TTTTCTGACA TCACGGGCAG GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCAGCTGGC 
TGGGCCTGGG CTGGAGACTG GTTCGTGTGT CCAGAGAAGA CACTGCTTCA CGACACTGAT 
GCTGGCCACC TGAGCTTCGT GGAGGAGGTA TTTGAGAACC AGACCCGGCT CCCTGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACG GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATCG AGTGCCCGCT GGGCTGGAAG TGGGAAGATG AAGAGTGGTC CACGGACCTG 
AATCGGGCTG TCGATGAACA AGGCTGGGAG TACAGCATCA CCATCCCGCC GGACCGGAAG 
CCGAGGCACT GGGTCCCTGC CGAGAAGATG TACTACACAC ACCGGCGGCG GCGCTGGGTC 
CGTCTGCGCC GGAGGGACCT CAGCCAGATG GAGGCGTTGA AGCGGCACAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCG CTCTTCGGCT GGAAGTTCCA TCTGGAGTAC 
CGCAAGACAG ACGCCTTTCG TCGTCGCCGC TGGCGCCGCC GCATGGAGCC CTTGGAGAAG 
ACAGGGCCTG CGGCTGTGTT TGCCCTTGAG GGGGCCCTGG GTGGCGTGGT GGACGACAGG 
AGTGAAGATT CCGTGTCCGT CTCCACCTTG AGTTTTGGTG TGAACAGACC CACGATTTCC 
TGCATCTTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAT 
CTGCCTGCAA TGGACAAGGA CTCTTTTTCC GATCCCTATG CAGTCGTCTC CTTCCTGCAC 
CAAAGCCAGA AGACGGTGGT GGCTAAGAAC ACCCTAAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCAAGCAGCA TCGCCGAGCA GCCTCCCAGC 
ATCGTGGTGG AGCTGTATGA CCATGACACC TATGGTGTAG ATGAGTTCAT GGGACGTTGC 
ATCTGTCAGC CGAGTCTGGA ACGGACGCCT CGGCTGGCCT GGTTCCCGCT GACGAGGGGC 
AGCCAGCCCG CCGGTGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCTGCC 
ATCCACCATA TTCCTGGTTT TGAGGTACAG GACACAACAG GGATCCTGGA AGAGCTGTGT 
GCACCTGCCT TCTTCCTCCA GGGCCTCCTG CCAACGTCAG AGGACACGGA CCTGCCCTAC 
CCACCGCCCC AGAGGGAGGC CAACATCTAC ATGGTCCCCC AGAACATCAA GCCGGTTCTC 
CAGCGAACCG CCATCGAGAT CCTGGCGTGG GGTCTTCGGA ACATGAAGAG CTACCAGCTG 
GCCAGCGTGT CCTCCCCGAG CCTGGTGGTG GAGTGCGGGG GCCAGTCTGT ACAATCCTGT 
GTCATCAAGA ACCTCCGGAA GAACCCCAAC TTCGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT GTACTGTCCC CCCATCGTGG TCAAGGTCAT TGATAACCGC 
CAGTTTGGCC GCCGGCCCGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GGGCTTCCTG 
TGTGATCCTT ACTCAGAGGA GAGTCCGTCC CCACAGGGTG GCCCAGATGA CGTGAGCTTA 
CTCAGTCCTG GGGAAGATGT GCTGATTGAC ATCGATGACA AGGAGCCCCT CATCCCCATC 
CAGTTTGCAG ATGGTCTCTC GGGCTTGGCC CCCACTAACA TGGCATCTTC TCCATCCAGT 
CTTCATAATC AGAGCAGGCT CCTGGGAGAT CCCTTAGTTG ACCGCCTCAT ACAGAGGAGG 
AAAGCAGGCC CCAGAATGGG CTTCCTGACG CCTTGTCCAG TGCCCTTCCC ATGGAACTGG 
GAGGAGGAGT TCATTGATTG GTGGAGCAAA TTCTTTGCTT CAATTGGGGA GAGCGAAAAG 
TGCGGGTCCT ACCTAGAGAA GGACTTTGAC ACTCTAAAGG TCTATGACAC ATCCCTGGAG 
AATGTGAAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAATA CATTCAAGCT CTACCGGGGC 
AAGACACAGG AGGAGACTGA AGACCCATCA GTCATTGGGG AGTTTAAGGG ACTCTTCAAA 
ATTTATCCTC TCCCAGAAGA CCCAGCCATC CCCTTGCCCC CGAGGCAGTT CCACCAGCTG 
GCTTCCCAGG GGCCCCAGGA ATGCCTGGTC CGCGTCTACA TTATCCGGGC GTTTGGCCTG 
CAGCCCAAGG ATCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCGGTGAGTG ACCAGGACAG CTACATCCCT TGTACTCTGG AGCCTGTATT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGGTCACCCT CTATGACTAT 
GACCTCCTCT CCAAGGATGA AAAGATTGGG GAGACGGTCA TTGACCTGGA GAACAGGCTG 
CTGTCTAAGT TCGGGGCCCG CTGTGGACTC CCCCAGACCT ACTGCGTCTC TGGACCCAAC 
CAGTGGAGGG ATCAGCTCCG CCCCTCCCAG CTCCTCCACC TGTTCTGCCA GCAGCACCGA 
GTCAAGGCCC CCGTGTACCG GACGGACCGC GTGGTGTTTC AGGATAAAGA ATACACCGTC 
GAAGAAATAG AGGCTGGCAG GGTCCCGAAC CCACACCTGG GCCCAGTGGA GGAGCGTCTG 
GCCTTGCACG TCCTCCAGCA GCAGGGGCTG ATCCCTGAGC ACGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCGGA CATCGAGCAG GGGAAGCTGC AGATGTGGGT CGACCTGTTT 
CCGAAGGCCC TGGGGCGGCC GGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAACACC AAGGATGTGA TCCTGGATGA CCTCAGCATC 
ACGGGGGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGGTTGGCTT TGAAGAACAC 
AAGCAAAAGA CAGATGTACA TTATCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCGTCTTCC CTTTTGACTA CCTGCCAGCT GAGCAGGTCT GCACCGTCTC CAAGAAGGAT 
GCATTCTGGA GGCTGGACAA GACGGAGAGC AAGATCCCTG CACGAGTGGT ATTCCAGATC 
TGGGACAACG ACAAGTTCTC CTTCGATGAC TTTCTGGGCT CTCTGCAGCT GGACCTTAAC 
CACATGCCCA AGCCCGCCAA GACAGCTGAG AAGTGCTCTG CGGACCAGCT GGAAGACACC 
TTCCACCCAG AGCGGTTTGT GTCTCTTTTT GAACAGAAAA CAGTGAAGGG CTGGTGGCCC 
TGTGTGGCGG AAGAGGGTGA GAAGAAGATA CTGGCGGGCA AGCTGGAGAT GACCTTGGAG 
ATTGTAACAG AGAGTGAGCA TGAGGAGCGG CCAGCCGGCC ACGGCCGGGA TGAACCTAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGG CCTGACACCT CCTTCCTCTG GTTCACCTCC 
CCGTACAAGA CCATGAAGTT CATCCTGTGG CGGCGGTTCC GCTGTGCCAT CATCTTCTTC 
CTCATTCTCT TCATCTTCCT GCTCTTCTTG GGCATCTTTG TCTACTCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328880.1
CDS	16..6528
Translation	MGACFPGQPVPQGAGKRKLSESCCSPEVPGTALSPHPLPETGEQSSHWRERSRRASCKRWDSRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEELCAPAFFLQGLLPTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHNQSRLLGDPLVDRLIQRRKAGPRMGFLTPCPVPFPWNWEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473394.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473394.1

ORF Insert Sequence:

ATGGGAGCGT GTTTCCCAGG ACAGCCTGTG CCCCAGGGAG CAGGGAAGAG AAAGCTCTCT 
GAGTCCTGCT GCAGCCCTGA GGTGCCTGGC ACGGCCCTCT CTCCCCACCC ACTGCCGGAG 
ACGGGCGAGC AGAGCAGCCA CTGGCGGGAG CGCTCCAGGA GGGCTTCATG CAAGAGGTGG 
GACTCGAGGT TCCTGGGGGA AGCCAACATC CCCCTCCGGG AAGTCCTCGC CACCCCCAGC 
CTGTCTGCCA GCTTCAATGC CCCACTGCTG GACACCAAGA AGCAGCCCAC AGGGGCCTCG 
CTGGTCCTGC AGGTGTCCTA CACGCCGCTG CCTGGAGCCG TGCCCACCTT CCCACCCTTA 
ACACCTCTGG AACCCTCCCC GACTCTGCCT GACATGGATA CGGTGGCTGG TGGGGGCCAG 
AGCCTGGCCG AGACCTGGTC CCTGCTCAGT GACAGCACCG TGGACACGAG ACACTCTGGA 
AGGAAGTGGC TGGCCCCCAC GGACACAGGA GGAGAGGAGG ACACTGAGGA CCAGGGGCTC 
ACGGGAGACG AGGCCGAGCC ATTCCTGGAC CAGAGCGGGG CTCTGGGCCC GGGGGCCCCC 
AGCACCCCGA AGAAACAACC TTCCCATCCG CCCCCCTACC ACCCCGGAGG GGGAAGGAAG 
AGAAGCGCGC CTGCGCCCAG CAAGCCTCTG TCGGACAAGC CGCAGGACTT CCAGATCAGG 
GTCCAGGTGA TCAAGGGACG CCAGCTGCCA GGGGTGAACA TCAAGCCTGT GGTCAAGGTC 
ACCGCCGCTG GGCAGACCAA GCGGACGCGG ATCCACAAAG GAAACAGCCC CGTCTTCAAT 
GAGACTCTCT TCTTCAACGT GTTTGACTCT CCCGCGGAGC TGTTCAATGA GCCCATCTTC 
ATCACGGTGG TGGACTCCCG TTCCCTCAGA ACAGATGCTC TCATCGGGGA GTTCCGGCTG 
GACGTGGGCA GCATCTACCG AGAGCCCCGA CATGCCTACC TCAGGAAGTG GCTGCTGCTC 
TCAGATCCCG ACGATTTCTC TGCTGGGGCC AGAGGCTACC TGAAAGCGAG TCTCTGTGTG 
CTGGGGCCTG GAGATGAAGC CCCCCTGGAG AGAAAAGACC CCTCTGAAGA CAAGGAGGAC 
ATCGAAAGCA ACCTGCTTAG GCCGATCGGC GTGGCCCTCC GTGGAGCGCA CTTCTGTCTG 
AAGGTCTTCA GGGCCGAGGA CTTGCCACAG ATGGACGATG CCGTGATGGA CAACGTGAGG 
CAGATCTTCG GCTTTGACAG TAACAAGAAG AACTTGGTGG ATCCCTTTGT GGAAGTCAGC 
TTTGCAGGGA AAATGCTCTG CAGCAAGATC CTGGAGAAGA CGGCCAATCC TCAGTGGAAC 
CAGAGCATCA CACTGCCTGC CATGTTTCCA TCCATGTGTG AGAAAATGAG GATTCGTGTC 
GTAGACTGGG ACCGCCTCAC CCACAACGAT ATTGTGGCCA CCACCTACCT GAGTATGTCG 
AAGATCTCTG CCTCTGGAGG CGAGATAGAA GAGGAGCCTG CAGGTGTGGT CAAGCCTCCT 
CCAGCCACAG AACTGGACGA CCATCTAGGC TTCCTCCCCA CCTTCGGGCC CTGCTACATC 
AACCTCTATG GCAGCCCCAG GGAGTTCACT GGCTTCCCGG ATCCCTATGC AGAGCTCAAC 
ACGGGCAAGG GAGAAGGCGT GGCCTACCGA GGCCGGCTCC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC GCAGTGAACA GAAAGTGGAG GCTCTCTCTG CCGATGACAT CCTTCGGGTA 
GAGAAGTACC TCCGGAGGCG CAAGTACTCG CTCTTTGCCG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TCGATGACGC CGTCCAGTTC GAGGTCAGCA TTGGGAACTA TGGCAACAAG 
TTCGACACCA CCTGCCTGCC GCTGGCCTCC ACCACCCAGT ACAGCCGGGC GGTCTTTGAT 
GGATGCCACT ACTACTACTT ACCCTGGGGC AACGTGAAGC CCGTGGTGGT GCTGTCGTCT 
TACTGGGAGG ACATCCGCCA CAGAGTTGAG GCTCAGAACC AGCTTCTCAG GATTGCCGAC 
CAGCTGGAAG CCGGCCTGGA GCAAGTCCAC TTGGCCCTGA AGGCTCAGTG CTCCGACGAG 
GACGCGGACT CCCTGGTGGC TCAGCTGATG GATGAGCTCA TCGCAGGCTG CAGCCAACCC 
CTGGGTGATG TCCAGGAGAT GCCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGCC ACCTGAGCCA GATCACCGAG GCGGCCCAGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCCG CTGCCCTGGA GCAGGCTGAG GACTGGCTCC TGCGGCTCCG TGCCCTGGCT 
GACGAGCCCC AGAACAGCCT GCCTGACATC GTCATCTGGA TGCTTCAGGG GGACAAGCGG 
GTGGCGTACC AGCGGGTGCC TGCCCATGAG GTCCTCTTCT CCCGCCGGGG CACCAGCTAC 
TGTGGCAAGA ACTGTGGAAA ACTGCAGACG ATATTTCTGA AATATCCGAT GGAAGGGGTG 
CCCAGAGCCC GGATGCCGGT GCAGATTCGG GTCAGGCTCT GGTTTGGACT CTCTGTGGAT 
GAAAAGGAGT TCAACCAGTT TGCTGAGGGC AAGCTCTCCG TCTTCGCTGA AACTTATGAG 
AACCAGACCA AGCTGGCCCT GGTTGGGAAC TGGGGCACAA CTGGCCTCAC CTACCCCAAG 
TTTTCTGACA TCACGGGCAG GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCAGCTGGC 
TGGGCCTGGG CTGGAGACTG GTTCGTGTGT CCAGAGAAGA CACTGCTTCA CGACACTGAT 
GCTGGCCACC TGAGCTTCGT GGAGGAGGTA TTTGAGAACC AGACCCGGCT CCCTGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACG GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATCG AGTGCCCGCT GGGCTGGAAG TGGGAAGATG AAGAGTGGTC CACGGACCTG 
AATCGGGCTG TCGATGAACA AGGCTGGGAG TACAGCATCA CCATCCCGCC GGACCGGAAG 
CCGAGGCACT GGGTCCCTGC CGAGAAGATG TACTACACAC ACCGGCGGCG GCGCTGGGTC 
CGTCTGCGCC GGAGGGACCT CAGCCAGATG GAGGCGTTGA AGCGGCACAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCG CTCTTCGGCT GGAAGTTCCA TCTGGAGTAC 
CGCAAGACAG ACGCCTTTCG TCGTCGCCGC TGGCGCCGCC GCATGGAGCC CTTGGAGAAG 
ACAGGGCCTG CGGCTGTGTT TGCCCTTGAG GGGGCCCTGG GTGGCGTGGT GGACGACAGG 
AGTGAAGATT CCGTGTCCGT CTCCACCTTG AGTTTTGGTG TGAACAGACC CACGATTTCC 
TGCATCTTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAT 
CTGCCTGCAA TGGACAAGGA CTCTTTTTCC GATCCCTATG CAGTCGTCTC CTTCCTGCAC 
CAAAGCCAGA AGACGGTGGT GGCTAAGAAC ACCCTAAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCAAGCAGCA TCGCCGAGCA GCCTCCCAGC 
ATCGTGGTGG AGCTGTATGA CCATGACACC TATGGTGTAG ATGAGTTCAT GGGACGTTGC 
ATCTGTCAGC CGAGTCTGGA ACGGACGCCT CGGCTGGCCT GGTTCCCGCT GACGAGGGGC 
AGCCAGCCCG CCGGTGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCTGCC 
ATCCACCATA TTCCTGGTTT TGAGGTACAG GACACAACAG GGATCCTGGA AGAGCTGTGT 
GCACCTGCCT TCTTCCTCCA GGGCCTCCTG CCAACGTCAG AGGACACGGA CCTGCCCTAC 
CCACCGCCCC AGAGGGAGGC CAACATCTAC ATGGTCCCCC AGAACATCAA GCCGGTTCTC 
CAGCGAACCG CCATCGAGAT CCTGGCGTGG GGTCTTCGGA ACATGAAGAG CTACCAGCTG 
GCCAGCGTGT CCTCCCCGAG CCTGGTGGTG GAGTGCGGGG GCCAGTCTGT ACAATCCTGT 
GTCATCAAGA ACCTCCGGAA GAACCCCAAC TTCGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT GTACTGTCCC CCCATCGTGG TCAAGGTCAT TGATAACCGC 
CAGTTTGGCC GCCGGCCCGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GGGCTTCCTG 
TGTGATCCTT ACTCAGAGGA GAGTCCGTCC CCACAGGGTG GCCCAGATGA CGTGAGCTTA 
CTCAGTCCTG GGGAAGATGT GCTGATTGAC ATCGATGACA AGGAGCCCCT CATCCCCATC 
CAGTTTGCAG ATGGTCTCTC GGGCTTGGCC CCCACTAACA TGGCATCTTC TCCATCCAGT 
CTTCATAATC AGAGCAGGCT CCTGGGAGAT CCCTTAGTTG ACCGCCTCAT ACAGAGGAGG 
AAAGCAGGCC CCAGAATGGG CTTCCTGACG CCTTGTCCAG TGCCCTTCCC ATGGAACTGG 
GAGGAGGAGT TCATTGATTG GTGGAGCAAA TTCTTTGCTT CAATTGGGGA GAGCGAAAAG 
TGCGGGTCCT ACCTAGAGAA GGACTTTGAC ACTCTAAAGG TCTATGACAC ATCCCTGGAG 
AATGTGAAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAATA CATTCAAGCT CTACCGGGGC 
AAGACACAGG AGGAGACTGA AGACCCATCA GTCATTGGGG AGTTTAAGGG ACTCTTCAAA 
ATTTATCCTC TCCCAGAAGA CCCAGCCATC CCCTTGCCCC CGAGGCAGTT CCACCAGCTG 
GCTTCCCAGG GGCCCCAGGA ATGCCTGGTC CGCGTCTACA TTATCCGGGC GTTTGGCCTG 
CAGCCCAAGG ATCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCGGTGAGTG ACCAGGACAG CTACATCCCT TGTACTCTGG AGCCTGTATT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGGTCACCCT CTATGACTAT 
GACCTCCTCT CCAAGGATGA AAAGATTGGG GAGACGGTCA TTGACCTGGA GAACAGGCTG 
CTGTCTAAGT TCGGGGCCCG CTGTGGACTC CCCCAGACCT ACTGCGTCTC TGGACCCAAC 
CAGTGGAGGG ATCAGCTCCG CCCCTCCCAG CTCCTCCACC TGTTCTGCCA GCAGCACCGA 
GTCAAGGCCC CCGTGTACCG GACGGACCGC GTGGTGTTTC AGGATAAAGA ATACACCGTC 
GAAGAAATAG AGGCTGGCAG GGTCCCGAAC CCACACCTGG GCCCAGTGGA GGAGCGTCTG 
GCCTTGCACG TCCTCCAGCA GCAGGGGCTG ATCCCTGAGC ACGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCGGA CATCGAGCAG GGGAAGCTGC AGATGTGGGT CGACCTGTTT 
CCGAAGGCCC TGGGGCGGCC GGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAACACC AAGGATGTGA TCCTGGATGA CCTCAGCATC 
ACGGGGGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGGTTGGCTT TGAAGAACAC 
AAGCAAAAGA CAGATGTACA TTATCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCGTCTTCC CTTTTGACTA CCTGCCAGCT GAGCAGGTCT GCACCGTCTC CAAGAAGGAT 
GCATTCTGGA GGCTGGACAA GACGGAGAGC AAGATCCCTG CACGAGTGGT ATTCCAGATC 
TGGGACAACG ACAAGTTCTC CTTCGATGAC TTTCTGGGCT CTCTGCAGCT GGACCTTAAC 
CACATGCCCA AGCCCGCCAA GACAGCTGAG AAGTGCTCTG CGGACCAGCT GGAAGACACC 
TTCCACCCAG AGCGGTTTGT GTCTCTTTTT GAACAGAAAA CAGTGAAGGG CTGGTGGCCC 
TGTGTGGCGG AAGAGGGTGA GAAGAAGATA CTGGCGGGCA AGCTGGAGAT GACCTTGGAG 
ATTGTAACAG AGAGTGAGCA TGAGGAGCGG CCAGCCGGCC ACGGCCGGGA TGAACCTAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGG CCTGACACCT CCTTCCTCTG GTTCACCTCC 
CCGTACAAGA CCATGAAGTT CATCCTGTGG CGGCGGTTCC GCTGTGCCAT CATCTTCTTC 
CTCATTCTCT TCATCTTCCT GCTCTTCTTG GGCATCTTTG TCTACTCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473395.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328881.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa91840
Clone ID Related Accession (Same CDS sequence)	XM_015473395.1
Accession Version	XM_015473395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTCCTCC AGCCACAGAA CTGGACGACC ATCTAGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACATCAA CCTCTATGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAT 
CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTCCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCGC AGTGAACAGA AAGTGGAGGC TCTCTCTGCC 
GATGACATCC TTCGGGTAGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT GCAGGATGTC GATGACGCCG TCCAGTTCGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACCACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGATGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC ATCCGCCACA GAGTTGAGGC TCAGAACCAG 
CTTCTCAGGA TTGCCGACCA GCTGGAAGCC GGCCTGGAGC AAGTCCACTT GGCCCTGAAG 
GCTCAGTGCT CCGACGAGGA CGCGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATC 
GCAGGCTGCA GCCAACCCCT GGGTGATGTC CAGGAGATGC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGT ACCAGCTGCG CACCCGCCAC CTGAGCCAGA TCACCGAGGC GGCCCAGGCC 
CTGAAGCTCG GCCACAGTGA GCTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTTCAGGGGG ACAAGCGGGT GGCGTACCAG CGGGTGCCTG CCCATGAGGT CCTCTTCTCC 
CGCCGGGGCA CCAGCTACTG TGGCAAGAAC TGTGGAAAAC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AAGGGGTGCC CAGAGCCCGG ATGCCGGTGC AGATTCGGGT CAGGCTCTGG 
TTTGGACTCT CTGTGGATGA AAAGGAGTTC AACCAGTTTG CTGAGGGCAA GCTCTCCGTC 
TTCGCTGAAA CTTATGAGAA CCAGACCAAG CTGGCCCTGG TTGGGAACTG GGGCACAACT 
GGCCTCACCT ACCCCAAGTT TTCTGACATC ACGGGCAGGA TCAAGCTACC CAAGGACAGC 
TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT GGAGACTGGT TCGTGTGTCC AGAGAAGACA 
CTGCTTCACG ACACTGATGC TGGCCACCTG AGCTTCGTGG AGGAGGTATT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTGAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCGCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC 
CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG AGGGACCTCA GCCAGATGGA GGCGTTGAAG 
CGGCACAGGC AGGCGGAGGC AGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG 
AAGTTCCATC TGGAGTACCG CAAGACAGAC GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC 
ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT 
GGCGTGGTGG ACGACAGGAG TGAAGATTCC GTGTCCGTCT CCACCTTGAG TTTTGGTGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT ACGCTGCTAC 
ATGTACCAGG CCCGGGATCT GCCTGCAATG GACAAGGACT CTTTTTCCGA TCCCTATGCA 
GTCGTCTCCT TCCTGCACCA AAGCCAGAAG ACGGTGGTGG CTAAGAACAC CCTAAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTACGAG ATCGAGATCT TTGGCGAGCC AAGCAGCATC 
GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA TGGTGTAGAT 
GAGTTCATGG GACGTTGCAT CTGTCAGCCG AGTCTGGAAC GGACGCCTCG GCTGGCCTGG 
TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCTGCCAT CCACCATATT CCTGGTTTTG AGGTACAGGA CACAACAGGG 
ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTGCC AACGTCAGAG 
GACACGGACC TGCCCTACCC ACCGCCCCAG AGGGAGGCCA ACATCTACAT GGTCCCCCAG 
AACATCAAGC CGGTTCTCCA GCGAACCGCC ATCGAGATCC TGGCGTGGGG TCTTCGGAAC 
ATGAAGAGCT ACCAGCTGGC CAGCGTGTCC TCCCCGAGCC TGGTGGTGGA GTGCGGGGGC 
CAGTCTGTAC AATCCTGTGT CATCAAGAAC CTCCGGAAGA ACCCCAACTT CGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTGT ACTGTCCCCC CATCGTGGTC 
AAGGTCATTG ATAACCGCCA GTTTGGCCGC CGGCCCGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGG GCTTCCTGTG TGATCCTTAC TCAGAGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGATGACG TGAGCTTACT CAGTCCTGGG GAAGATGTGC TGATTGACAT CGATGACAAG 
GAGCCCCTCA TCCCCATCCA GTTTGCAGAT GGTCTCTCGG GCTTGGCCCC CACTAACATG 
GCATCTTCTC CATCCAGTCT TCATAATCAG AGCAGGCTCC TGGGAGATCC CTTAGTTGAC 
CGCCTCATAC AGAGGAGGAA AGCAGGCCCC AGAATGGGCT TCCTGACGCC TTGTCCAGTG 
CCCTTCCCAT GGAACTGGGA GGAGGAGTTC ATTGATTGGT GGAGCAAATT CTTTGCTTCA 
ATTGGGGAGA GCGAAAAGTG CGGGTCCTAC CTAGAGAAGG ACTTTGACAC TCTAAAGGTC 
TATGACACAT CCCTGGAGAA TGTGAAGGCC TTTGAGGGCC TGTCTGACTT TTGTAATACA 
TTCAAGCTCT ACCGGGGCAA GACACAGGAG GAGACTGAAG ACCCATCAGT CATTGGGGAG 
TTTAAGGGAC TCTTCAAAAT TTATCCTCTC CCAGAAGACC CAGCCATCCC CTTGCCCCCG 
AGGCAGTTCC ACCAGCTGGC TTCCCAGGGG CCCCAGGAAT GCCTGGTCCG CGTCTACATT 
ATCCGGGCGT TTGGCCTGCA GCCCAAGGAT CCCAATGGGA AGTGTGATCC TTACATCAAG 
ATCTCCATAG GGAAGAAATC GGTGAGTGAC CAGGACAGCT ACATCCCTTG TACTCTGGAG 
CCTGTATTTG GAAAGATGTT CGAGCTGACC TGCACTCTGC CTCTGGAGAA GGACCTGAAG 
GTCACCCTCT ATGACTATGA CCTCCTCTCC AAGGATGAAA AGATTGGGGA GACGGTCATT 
GACCTGGAGA ACAGGCTGCT GTCTAAGTTC GGGGCCCGCT GTGGACTCCC CCAGACCTAC 
TGCGTCTCTG GACCCAACCA GTGGAGGGAT CAGCTCCGCC CCTCCCAGCT CCTCCACCTG 
TTCTGCCAGC AGCACCGAGT CAAGGCCCCC GTGTACCGGA CGGACCGCGT GGTGTTTCAG 
GATAAAGAAT ACACCGTCGA AGAAATAGAG GCTGGCAGGG TCCCGAACCC ACACCTGGGC 
CCAGTGGAGG AGCGTCTGGC CTTGCACGTC CTCCAGCAGC AGGGGCTGAT CCCTGAGCAC 
GTGGAGTCCC GGCCTCTCTA CAGCCCCCTG CAGCCGGACA TCGAGCAGGG GAAGCTGCAG 
ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG GGGCGGCCGG GACCTCCCTT CAACATCACC 
CCACGGAGAG CCAGAAGGTT TTTCCTGCGT TGTATTATCT GGAACACCAA GGATGTGATC 
CTGGATGACC TCAGCATCAC GGGGGAGAAG ATGAGCGACA TTTATGTGAA AGGTTGGATG 
GTTGGCTTTG AAGAACACAA GCAAAAGACA GATGTACATT ATCGGTCCCT GGGAGGTGAA 
GGCAACTTTA ACTGGAGGTT CGTCTTCCCT TTTGACTACC TGCCAGCTGA GCAGGTCTGC 
ACCGTCTCCA AGAAGGATGC ATTCTGGAGG CTGGACAAGA CGGAGAGCAA GATCCCTGCA 
CGAGTGGTAT TCCAGATCTG GGACAACGAC AAGTTCTCCT TCGATGACTT TCTGGGCTCT 
CTGCAGCTGG ACCTTAACCA CATGCCCAAG CCCGCCAAGA CAGCTGAGAA GTGCTCTGCG 
GACCAGCTGG AAGACACCTT CCACCCAGAG CGGTTTGTGT CTCTTTTTGA ACAGAAAACA 
GTGAAGGGCT GGTGGCCCTG TGTGGCGGAA GAGGGTGAGA AGAAGATACT GGCGGGCAAG 
CTGGAGATGA CCTTGGAGAT TGTAACAGAG AGTGAGCATG AGGAGCGGCC AGCCGGCCAC 
GGCCGGGATG AACCTAACAT GAACCCCAAG CTCGAGGACC CAAGGCGGCC TGACACCTCC 
TTCCTCTGGT TCACCTCCCC GTACAAGACC ATGAAGTTCA TCCTGTGGCG GCGGTTCCGC 
TGTGCCATCA TCTTCTTCCT CATTCTCTTC ATCTTCCTGC TCTTCTTGGG CATCTTTGTC 
TACTCCTTCC CGAACTATGC TGCCATGAAG CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328881.1
CDS	131..6601
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEELCAPAFFLQGLLPTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHNQSRLLGDPLVDRLIQRRKAGPRMGFLTPCPVPFPWNWEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473395.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473395.1

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTCCTCC AGCCACAGAA CTGGACGACC ATCTAGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACATCAA CCTCTATGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAT 
CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTCCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCGC AGTGAACAGA AAGTGGAGGC TCTCTCTGCC 
GATGACATCC TTCGGGTAGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT GCAGGATGTC GATGACGCCG TCCAGTTCGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACCACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGATGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC ATCCGCCACA GAGTTGAGGC TCAGAACCAG 
CTTCTCAGGA TTGCCGACCA GCTGGAAGCC GGCCTGGAGC AAGTCCACTT GGCCCTGAAG 
GCTCAGTGCT CCGACGAGGA CGCGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATC 
GCAGGCTGCA GCCAACCCCT GGGTGATGTC CAGGAGATGC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGT ACCAGCTGCG CACCCGCCAC CTGAGCCAGA TCACCGAGGC GGCCCAGGCC 
CTGAAGCTCG GCCACAGTGA GCTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTTCAGGGGG ACAAGCGGGT GGCGTACCAG CGGGTGCCTG CCCATGAGGT CCTCTTCTCC 
CGCCGGGGCA CCAGCTACTG TGGCAAGAAC TGTGGAAAAC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AAGGGGTGCC CAGAGCCCGG ATGCCGGTGC AGATTCGGGT CAGGCTCTGG 
TTTGGACTCT CTGTGGATGA AAAGGAGTTC AACCAGTTTG CTGAGGGCAA GCTCTCCGTC 
TTCGCTGAAA CTTATGAGAA CCAGACCAAG CTGGCCCTGG TTGGGAACTG GGGCACAACT 
GGCCTCACCT ACCCCAAGTT TTCTGACATC ACGGGCAGGA TCAAGCTACC CAAGGACAGC 
TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT GGAGACTGGT TCGTGTGTCC AGAGAAGACA 
CTGCTTCACG ACACTGATGC TGGCCACCTG AGCTTCGTGG AGGAGGTATT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTGAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCGCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC 
CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG AGGGACCTCA GCCAGATGGA GGCGTTGAAG 
CGGCACAGGC AGGCGGAGGC AGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG 
AAGTTCCATC TGGAGTACCG CAAGACAGAC GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC 
ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT 
GGCGTGGTGG ACGACAGGAG TGAAGATTCC GTGTCCGTCT CCACCTTGAG TTTTGGTGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT ACGCTGCTAC 
ATGTACCAGG CCCGGGATCT GCCTGCAATG GACAAGGACT CTTTTTCCGA TCCCTATGCA 
GTCGTCTCCT TCCTGCACCA AAGCCAGAAG ACGGTGGTGG CTAAGAACAC CCTAAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTACGAG ATCGAGATCT TTGGCGAGCC AAGCAGCATC 
GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA TGGTGTAGAT 
GAGTTCATGG GACGTTGCAT CTGTCAGCCG AGTCTGGAAC GGACGCCTCG GCTGGCCTGG 
TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCTGCCAT CCACCATATT CCTGGTTTTG AGGTACAGGA CACAACAGGG 
ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTGCC AACGTCAGAG 
GACACGGACC TGCCCTACCC ACCGCCCCAG AGGGAGGCCA ACATCTACAT GGTCCCCCAG 
AACATCAAGC CGGTTCTCCA GCGAACCGCC ATCGAGATCC TGGCGTGGGG TCTTCGGAAC 
ATGAAGAGCT ACCAGCTGGC CAGCGTGTCC TCCCCGAGCC TGGTGGTGGA GTGCGGGGGC 
CAGTCTGTAC AATCCTGTGT CATCAAGAAC CTCCGGAAGA ACCCCAACTT CGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTGT ACTGTCCCCC CATCGTGGTC 
AAGGTCATTG ATAACCGCCA GTTTGGCCGC CGGCCCGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGG GCTTCCTGTG TGATCCTTAC TCAGAGGAGA GTCCGTCCCC ACAGGGTGGC 
CCAGATGACG TGAGCTTACT CAGTCCTGGG GAAGATGTGC TGATTGACAT CGATGACAAG 
GAGCCCCTCA TCCCCATCCA GTTTGCAGAT GGTCTCTCGG GCTTGGCCCC CACTAACATG 
GCATCTTCTC CATCCAGTCT TCATAATCAG AGCAGGCTCC TGGGAGATCC CTTAGTTGAC 
CGCCTCATAC AGAGGAGGAA AGCAGGCCCC AGAATGGGCT TCCTGACGCC TTGTCCAGTG 
CCCTTCCCAT GGAACTGGGA GGAGGAGTTC ATTGATTGGT GGAGCAAATT CTTTGCTTCA 
ATTGGGGAGA GCGAAAAGTG CGGGTCCTAC CTAGAGAAGG ACTTTGACAC TCTAAAGGTC 
TATGACACAT CCCTGGAGAA TGTGAAGGCC TTTGAGGGCC TGTCTGACTT TTGTAATACA 
TTCAAGCTCT ACCGGGGCAA GACACAGGAG GAGACTGAAG ACCCATCAGT CATTGGGGAG 
TTTAAGGGAC TCTTCAAAAT TTATCCTCTC CCAGAAGACC CAGCCATCCC CTTGCCCCCG 
AGGCAGTTCC ACCAGCTGGC TTCCCAGGGG CCCCAGGAAT GCCTGGTCCG CGTCTACATT 
ATCCGGGCGT TTGGCCTGCA GCCCAAGGAT CCCAATGGGA AGTGTGATCC TTACATCAAG 
ATCTCCATAG GGAAGAAATC GGTGAGTGAC CAGGACAGCT ACATCCCTTG TACTCTGGAG 
CCTGTATTTG GAAAGATGTT CGAGCTGACC TGCACTCTGC CTCTGGAGAA GGACCTGAAG 
GTCACCCTCT ATGACTATGA CCTCCTCTCC AAGGATGAAA AGATTGGGGA GACGGTCATT 
GACCTGGAGA ACAGGCTGCT GTCTAAGTTC GGGGCCCGCT GTGGACTCCC CCAGACCTAC 
TGCGTCTCTG GACCCAACCA GTGGAGGGAT CAGCTCCGCC CCTCCCAGCT CCTCCACCTG 
TTCTGCCAGC AGCACCGAGT CAAGGCCCCC GTGTACCGGA CGGACCGCGT GGTGTTTCAG 
GATAAAGAAT ACACCGTCGA AGAAATAGAG GCTGGCAGGG TCCCGAACCC ACACCTGGGC 
CCAGTGGAGG AGCGTCTGGC CTTGCACGTC CTCCAGCAGC AGGGGCTGAT CCCTGAGCAC 
GTGGAGTCCC GGCCTCTCTA CAGCCCCCTG CAGCCGGACA TCGAGCAGGG GAAGCTGCAG 
ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG GGGCGGCCGG GACCTCCCTT CAACATCACC 
CCACGGAGAG CCAGAAGGTT TTTCCTGCGT TGTATTATCT GGAACACCAA GGATGTGATC 
CTGGATGACC TCAGCATCAC GGGGGAGAAG ATGAGCGACA TTTATGTGAA AGGTTGGATG 
GTTGGCTTTG AAGAACACAA GCAAAAGACA GATGTACATT ATCGGTCCCT GGGAGGTGAA 
GGCAACTTTA ACTGGAGGTT CGTCTTCCCT TTTGACTACC TGCCAGCTGA GCAGGTCTGC 
ACCGTCTCCA AGAAGGATGC ATTCTGGAGG CTGGACAAGA CGGAGAGCAA GATCCCTGCA 
CGAGTGGTAT TCCAGATCTG GGACAACGAC AAGTTCTCCT TCGATGACTT TCTGGGCTCT 
CTGCAGCTGG ACCTTAACCA CATGCCCAAG CCCGCCAAGA CAGCTGAGAA GTGCTCTGCG 
GACCAGCTGG AAGACACCTT CCACCCAGAG CGGTTTGTGT CTCTTTTTGA ACAGAAAACA 
GTGAAGGGCT GGTGGCCCTG TGTGGCGGAA GAGGGTGAGA AGAAGATACT GGCGGGCAAG 
CTGGAGATGA CCTTGGAGAT TGTAACAGAG AGTGAGCATG AGGAGCGGCC AGCCGGCCAC 
GGCCGGGATG AACCTAACAT GAACCCCAAG CTCGAGGACC CAAGGCGGCC TGACACCTCC 
TTCCTCTGGT TCACCTCCCC GTACAAGACC ATGAAGTTCA TCCTGTGGCG GCGGTTCCGC 
TGTGCCATCA TCTTCTTCCT CATTCTCTTC ATCTTCCTGC TCTTCTTGGG CATCTTTGTC 
TACTCCTTCC CGAACTATGC TGCCATGAAG CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473396.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328882.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa91841
Clone ID Related Accession (Same CDS sequence)	XM_015473396.1
Accession Version	XM_015473396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGC TGTGTGCACC TGCCTTCTTC CTCCAGGGCC TCCTGCCAAC GTCAGAGGAC 
ACGGACCTGC CCTACCCACC GCCCCAGAGG GAGGCCAACA TCTACATGGT CCCCCAGAAC 
ATCAAGCCGG TTCTCCAGCG AACCGCCATC GAGATCCTGG CGTGGGGTCT TCGGAACATG 
AAGAGCTACC AGCTGGCCAG CGTGTCCTCC CCGAGCCTGG TGGTGGAGTG CGGGGGCCAG 
TCTGTACAAT CCTGTGTCAT CAAGAACCTC CGGAAGAACC CCAACTTCGA CATCTGCACC 
CTCTTCATGG AAGTGATGCT GCCCAGGGAG GAGCTGTACT GTCCCCCCAT CGTGGTCAAG 
GTCATTGATA ACCGCCAGTT TGGCCGCCGG CCCGTGGTGG GCCAGTGTAC CATCCGCTCC 
CTGGAGGGCT TCCTGTGTGA TCCTTACTCA GAGGAGAGTC CGTCCCCACA GGGTGGCCCA 
GATGACGTGA GCTTACTCAG TCCTGGGGAA GATGTGCTGA TTGACATCGA TGACAAGGAG 
CCCCTCATCC CCATCCAGTT TGCAGATGGT CTCTCGGGCT TGGCCCCCAC TAACATGGCA 
TCTTCTCCAT CCAGTCTTCA TAATCAGAGC AGGCTCCTGG GAGATCCCTT AGTTGACCGC 
CTCATACAGA GGAGGAAAGC AGGCCCCAGA ATGGGCTTCC TGACGCCTTG TCCAGTGCCC 
TTCCCATGGA ACTGGGAGGA GGAGTTCATT GATTGGTGGA GCAAATTCTT TGCTTCAATT 
GGGGAGAGCG AAAAGTGCGG GTCCTACCTA GAGAAGGACT TTGACACTCT AAAGGTCTAT 
GACACATCCC TGGAGAATGT GAAGGCCTTT GAGGGCCTGT CTGACTTTTG TAATACATTC 
AAGCTCTACC GGGGCAAGAC ACAGGAGGAG ACTGAAGACC CATCAGTCAT TGGGGAGTTT 
AAGGGACTCT TCAAAATTTA TCCTCTCCCA GAAGACCCAG CCATCCCCTT GCCCCCGAGG 
CAGTTCCACC AGCTGGCTTC CCAGGGGCCC CAGGAATGCC TGGTCCGCGT CTACATTATC 
CGGGCGTTTG GCCTGCAGCC CAAGGATCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCGGT GAGTGACCAG GACAGCTACA TCCCTTGTAC TCTGGAGCCT 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC 
ACCCTCTATG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGGGAGAC GGTCATTGAC 
CTGGAGAACA GGCTGCTGTC TAAGTTCGGG GCCCGCTGTG GACTCCCCCA GACCTACTGC 
GTCTCTGGAC CCAACCAGTG GAGGGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTGTTC 
TGCCAGCAGC ACCGAGTCAA GGCCCCCGTG TACCGGACGG ACCGCGTGGT GTTTCAGGAT 
AAAGAATACA CCGTCGAAGA AATAGAGGCT GGCAGGGTCC CGAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCAGG GGCTGATCCC TGAGCACGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCGGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGGCCGGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACCAAGGA TGTGATCCTG 
GATGACCTCA GCATCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT 
GGCTTTGAAG AACACAAGCA AAAGACAGAT GTACATTATC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCGT CTTCCCTTTT GACTACCTGC CAGCTGAGCA GGTCTGCACC 
GTCTCCAAGA AGGATGCATT CTGGAGGCTG GACAAGACGG AGAGCAAGAT CCCTGCACGA 
GTGGTATTCC AGATCTGGGA CAACGACAAG TTCTCCTTCG ATGACTTTCT GGGCTCTCTG 
CAGCTGGACC TTAACCACAT GCCCAAGCCC GCCAAGACAG CTGAGAAGTG CTCTGCGGAC 
CAGCTGGAAG ACACCTTCCA CCCAGAGCGG TTTGTGTCTC TTTTTGAACA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAGATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCAGC CGGCCACGGC 
CGGGATGAAC CTAACATGAA CCCCAAGCTC GAGGACCCAA GGCGGCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGCTGT 
GCCATCATCT TCTTCCTCAT TCTCTTCATC TTCCTGCTCT TCTTGGGCAT CTTTGTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328882.1
CDS	544..7011
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEELCAPAFFLQGLLPTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHNQSRLLGDPLVDRLIQRRKAGPRMGFLTPCPVPFPWNWEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473396.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473396.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGC TGTGTGCACC TGCCTTCTTC CTCCAGGGCC TCCTGCCAAC GTCAGAGGAC 
ACGGACCTGC CCTACCCACC GCCCCAGAGG GAGGCCAACA TCTACATGGT CCCCCAGAAC 
ATCAAGCCGG TTCTCCAGCG AACCGCCATC GAGATCCTGG CGTGGGGTCT TCGGAACATG 
AAGAGCTACC AGCTGGCCAG CGTGTCCTCC CCGAGCCTGG TGGTGGAGTG CGGGGGCCAG 
TCTGTACAAT CCTGTGTCAT CAAGAACCTC CGGAAGAACC CCAACTTCGA CATCTGCACC 
CTCTTCATGG AAGTGATGCT GCCCAGGGAG GAGCTGTACT GTCCCCCCAT CGTGGTCAAG 
GTCATTGATA ACCGCCAGTT TGGCCGCCGG CCCGTGGTGG GCCAGTGTAC CATCCGCTCC 
CTGGAGGGCT TCCTGTGTGA TCCTTACTCA GAGGAGAGTC CGTCCCCACA GGGTGGCCCA 
GATGACGTGA GCTTACTCAG TCCTGGGGAA GATGTGCTGA TTGACATCGA TGACAAGGAG 
CCCCTCATCC CCATCCAGTT TGCAGATGGT CTCTCGGGCT TGGCCCCCAC TAACATGGCA 
TCTTCTCCAT CCAGTCTTCA TAATCAGAGC AGGCTCCTGG GAGATCCCTT AGTTGACCGC 
CTCATACAGA GGAGGAAAGC AGGCCCCAGA ATGGGCTTCC TGACGCCTTG TCCAGTGCCC 
TTCCCATGGA ACTGGGAGGA GGAGTTCATT GATTGGTGGA GCAAATTCTT TGCTTCAATT 
GGGGAGAGCG AAAAGTGCGG GTCCTACCTA GAGAAGGACT TTGACACTCT AAAGGTCTAT 
GACACATCCC TGGAGAATGT GAAGGCCTTT GAGGGCCTGT CTGACTTTTG TAATACATTC 
AAGCTCTACC GGGGCAAGAC ACAGGAGGAG ACTGAAGACC CATCAGTCAT TGGGGAGTTT 
AAGGGACTCT TCAAAATTTA TCCTCTCCCA GAAGACCCAG CCATCCCCTT GCCCCCGAGG 
CAGTTCCACC AGCTGGCTTC CCAGGGGCCC CAGGAATGCC TGGTCCGCGT CTACATTATC 
CGGGCGTTTG GCCTGCAGCC CAAGGATCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCGGT GAGTGACCAG GACAGCTACA TCCCTTGTAC TCTGGAGCCT 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC 
ACCCTCTATG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGGGAGAC GGTCATTGAC 
CTGGAGAACA GGCTGCTGTC TAAGTTCGGG GCCCGCTGTG GACTCCCCCA GACCTACTGC 
GTCTCTGGAC CCAACCAGTG GAGGGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTGTTC 
TGCCAGCAGC ACCGAGTCAA GGCCCCCGTG TACCGGACGG ACCGCGTGGT GTTTCAGGAT 
AAAGAATACA CCGTCGAAGA AATAGAGGCT GGCAGGGTCC CGAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCAGG GGCTGATCCC TGAGCACGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCGGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGGCCGGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACCAAGGA TGTGATCCTG 
GATGACCTCA GCATCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT 
GGCTTTGAAG AACACAAGCA AAAGACAGAT GTACATTATC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCGT CTTCCCTTTT GACTACCTGC CAGCTGAGCA GGTCTGCACC 
GTCTCCAAGA AGGATGCATT CTGGAGGCTG GACAAGACGG AGAGCAAGAT CCCTGCACGA 
GTGGTATTCC AGATCTGGGA CAACGACAAG TTCTCCTTCG ATGACTTTCT GGGCTCTCTG 
CAGCTGGACC TTAACCACAT GCCCAAGCCC GCCAAGACAG CTGAGAAGTG CTCTGCGGAC 
CAGCTGGAAG ACACCTTCCA CCCAGAGCGG TTTGTGTCTC TTTTTGAACA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAGATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCAGC CGGCCACGGC 
CGGGATGAAC CTAACATGAA CCCCAAGCTC GAGGACCCAA GGCGGCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGCTGT 
GCCATCATCT TCTTCCTCAT TCTCTTCATC TTCCTGCTCT TCTTGGGCAT CTTTGTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473397.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328883.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa91842
Clone ID Related Accession (Same CDS sequence)	XM_015473397.1
Accession Version	XM_015473397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT GCCAACGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGTTTGCA 
GATGGTCTCT CGGGCTTGGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCTTCATGAG 
GAGGAGTTCA TTGATTGGTG GAGCAAATTC TTTGCTTCAA TTGGGGAGAG CGAAAAGTGC 
GGGTCCTACC TAGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACATC CCTGGAGAAT 
GTGAAGGCCT TTGAGGGCCT GTCTGACTTT TGTAATACAT TCAAGCTCTA CCGGGGCAAG 
ACACAGGAGG AGACTGAAGA CCCATCAGTC ATTGGGGAGT TTAAGGGACT CTTCAAAATT 
TATCCTCTCC CAGAAGACCC AGCCATCCCC TTGCCCCCGA GGCAGTTCCA CCAGCTGGCT 
TCCCAGGGGC CCCAGGAATG CCTGGTCCGC GTCTACATTA TCCGGGCGTT TGGCCTGCAG 
CCCAAGGATC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGTGACC AGGACAGCTA CATCCCTTGT ACTCTGGAGC CTGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGG TCACCCTCTA TGACTATGAC 
CTCCTCTCCA AGGATGAAAA GATTGGGGAG ACGGTCATTG ACCTGGAGAA CAGGCTGCTG 
TCTAAGTTCG GGGCCCGCTG TGGACTCCCC CAGACCTACT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTGT TCTGCCAGCA GCACCGAGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCGTCGAA 
GAAATAGAGG CTGGCAGGGT CCCGAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCA GGGGCTGATC CCTGAGCACG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATCC TGGATGACCT CAGCATCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ATGTACATTA TCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
GTCTTCCCTT TTGACTACCT GCCAGCTGAG CAGGTCTGCA CCGTCTCCAA GAAGGATGCA 
TTCTGGAGGC TGGACAAGAC GGAGAGCAAG ATCCCTGCAC GAGTGGTATT CCAGATCTGG 
GACAACGACA AGTTCTCCTT CGATGACTTT CTGGGCTCTC TGCAGCTGGA CCTTAACCAC 
ATGCCCAAGC CCGCCAAGAC AGCTGAGAAG TGCTCTGCGG ACCAGCTGGA AGACACCTTC 
CACCCAGAGC GGTTTGTGTC TCTTTTTGAA CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTGGCGGAAG AGGGTGAGAA GAAGATACTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT 
GTAACAGAGA GTGAGCATGA GGAGCGGCCA GCCGGCCACG GCCGGGATGA ACCTAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGGCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGCT GTGCCATCAT CTTCTTCCTC 
ATTCTCTTCA TCTTCCTGCT CTTCTTGGGC ATCTTTGTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328883.1
CDS	130..6579
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEELCAPAFFLQGLLPTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473397.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473397.1

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT GCCAACGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGTTTGCA 
GATGGTCTCT CGGGCTTGGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCTTCATGAG 
GAGGAGTTCA TTGATTGGTG GAGCAAATTC TTTGCTTCAA TTGGGGAGAG CGAAAAGTGC 
GGGTCCTACC TAGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACATC CCTGGAGAAT 
GTGAAGGCCT TTGAGGGCCT GTCTGACTTT TGTAATACAT TCAAGCTCTA CCGGGGCAAG 
ACACAGGAGG AGACTGAAGA CCCATCAGTC ATTGGGGAGT TTAAGGGACT CTTCAAAATT 
TATCCTCTCC CAGAAGACCC AGCCATCCCC TTGCCCCCGA GGCAGTTCCA CCAGCTGGCT 
TCCCAGGGGC CCCAGGAATG CCTGGTCCGC GTCTACATTA TCCGGGCGTT TGGCCTGCAG 
CCCAAGGATC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGTGACC AGGACAGCTA CATCCCTTGT ACTCTGGAGC CTGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGG TCACCCTCTA TGACTATGAC 
CTCCTCTCCA AGGATGAAAA GATTGGGGAG ACGGTCATTG ACCTGGAGAA CAGGCTGCTG 
TCTAAGTTCG GGGCCCGCTG TGGACTCCCC CAGACCTACT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTGT TCTGCCAGCA GCACCGAGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCGTCGAA 
GAAATAGAGG CTGGCAGGGT CCCGAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCA GGGGCTGATC CCTGAGCACG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATCC TGGATGACCT CAGCATCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ATGTACATTA TCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
GTCTTCCCTT TTGACTACCT GCCAGCTGAG CAGGTCTGCA CCGTCTCCAA GAAGGATGCA 
TTCTGGAGGC TGGACAAGAC GGAGAGCAAG ATCCCTGCAC GAGTGGTATT CCAGATCTGG 
GACAACGACA AGTTCTCCTT CGATGACTTT CTGGGCTCTC TGCAGCTGGA CCTTAACCAC 
ATGCCCAAGC CCGCCAAGAC AGCTGAGAAG TGCTCTGCGG ACCAGCTGGA AGACACCTTC 
CACCCAGAGC GGTTTGTGTC TCTTTTTGAA CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTGGCGGAAG AGGGTGAGAA GAAGATACTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT 
GTAACAGAGA GTGAGCATGA GGAGCGGCCA GCCGGCCACG GCCGGGATGA ACCTAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGGCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGCT GTGCCATCAT CTTCTTCCTC 
ATTCTCTTCA TCTTCCTGCT CTTCTTGGGC ATCTTTGTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473398.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328884.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa91843
Clone ID Related Accession (Same CDS sequence)	XM_015473398.1
Accession Version	XM_015473398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGT CAGAGGACAC GGACCTGCCC TACCCACCGC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CCGCCATCGA GATCCTGGCG 
TGGGGTCTTC GGAACATGAA GAGCTACCAG CTGGCCAGCG TGTCCTCCCC GAGCCTGGTG 
GTGGAGTGCG GGGGCCAGTC TGTACAATCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTGTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC CGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGGGCTTC CTGTGTGATC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GTGGCCCAGA TGACGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTGATT 
GACATCGATG ACAAGGAGCC CCTCATCCCC ATCCAGTTTG CAGATGGTCT CTCGGGCTTG 
GCCCCCACTA ACATGGCATC TTCTCCATCC AGTCTTCATA ATCAGAGCAG GCTCCTGGGA 
GATCCCTTAG TTGACCGCCT CATACAGAGG AGGAAAGCAG GCCCCAGAAT GGGCTTCCTG 
ACGCCTTGTC CAGTGCCCTT CCCATGGAAC TGGGAGGAGG AGTTCATTGA TTGGTGGAGC 
AAATTCTTTG CTTCAATTGG GGAGAGCGAA AAGTGCGGGT CCTACCTAGA GAAGGACTTT 
GACACTCTAA AGGTCTATGA CACATCCCTG GAGAATGTGA AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ATACATTCAA GCTCTACCGG GGCAAGACAC AGGAGGAGAC TGAAGACCCA 
TCAGTCATTG GGGAGTTTAA GGGACTCTTC AAAATTTATC CTCTCCCAGA AGACCCAGCC 
ATCCCCTTGC CCCCGAGGCA GTTCCACCAG CTGGCTTCCC AGGGGCCCCA GGAATGCCTG 
GTCCGCGTCT ACATTATCCG GGCGTTTGGC CTGCAGCCCA AGGATCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCGGTGA GTGACCAGGA CAGCTACATC 
CCTTGTACTC TGGAGCCTGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC CCTCTATGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGGGAGACGG TCATTGACCT GGAGAACAGG CTGCTGTCTA AGTTCGGGGC CCGCTGTGGA 
CTCCCCCAGA CCTACTGCGT CTCTGGACCC AACCAGTGGA GGGATCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTGTTCTG CCAGCAGCAC CGAGTCAAGG CCCCCGTGTA CCGGACGGAC 
CGCGTGGTGT TTCAGGATAA AGAATACACC GTCGAAGAAA TAGAGGCTGG CAGGGTCCCG 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCCTTGC ACGTCCTCCA GCAGCAGGGG 
CTGATCCCTG AGCACGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC GGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCGGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAC 
ACCAAGGATG TGATCCTGGA TGACCTCAGC ATCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGGTTGG CTTTGAAGAA CACAAGCAAA AGACAGATGT ACATTATCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCGTCT TCCCTTTTGA CTACCTGCCA 
GCTGAGCAGG TCTGCACCGT CTCCAAGAAG GATGCATTCT GGAGGCTGGA CAAGACGGAG 
AGCAAGATCC CTGCACGAGT GGTATTCCAG ATCTGGGACA ACGACAAGTT CTCCTTCGAT 
GACTTTCTGG GCTCTCTGCA GCTGGACCTT AACCACATGC CCAAGCCCGC CAAGACAGCT 
GAGAAGTGCT CTGCGGACCA GCTGGAAGAC ACCTTCCACC CAGAGCGGTT TGTGTCTCTT 
TTTGAACAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTGG CGGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA GATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCAGCCG GCCACGGCCG GGATGAACCT AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGGCCTGACA CCTCCTTCCT CTGGTTCACC TCCCCGTACA AGACCATGAA GTTCATCCTG 
TGGCGGCGGT TCCGCTGTGC CATCATCTTC TTCCTCATTC TCTTCATCTT CCTGCTCTTC 
TTGGGCATCT TTGTCTACTC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328884.1
CDS	542..6967
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHNQSRLLGDPLVDRLIQRRKAGPRMGFLTPCPVPFPWNWEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473398.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473398.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGT CAGAGGACAC GGACCTGCCC TACCCACCGC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CCGCCATCGA GATCCTGGCG 
TGGGGTCTTC GGAACATGAA GAGCTACCAG CTGGCCAGCG TGTCCTCCCC GAGCCTGGTG 
GTGGAGTGCG GGGGCCAGTC TGTACAATCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTGTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC CGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGGGCTTC CTGTGTGATC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GTGGCCCAGA TGACGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTGATT 
GACATCGATG ACAAGGAGCC CCTCATCCCC ATCCAGTTTG CAGATGGTCT CTCGGGCTTG 
GCCCCCACTA ACATGGCATC TTCTCCATCC AGTCTTCATA ATCAGAGCAG GCTCCTGGGA 
GATCCCTTAG TTGACCGCCT CATACAGAGG AGGAAAGCAG GCCCCAGAAT GGGCTTCCTG 
ACGCCTTGTC CAGTGCCCTT CCCATGGAAC TGGGAGGAGG AGTTCATTGA TTGGTGGAGC 
AAATTCTTTG CTTCAATTGG GGAGAGCGAA AAGTGCGGGT CCTACCTAGA GAAGGACTTT 
GACACTCTAA AGGTCTATGA CACATCCCTG GAGAATGTGA AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ATACATTCAA GCTCTACCGG GGCAAGACAC AGGAGGAGAC TGAAGACCCA 
TCAGTCATTG GGGAGTTTAA GGGACTCTTC AAAATTTATC CTCTCCCAGA AGACCCAGCC 
ATCCCCTTGC CCCCGAGGCA GTTCCACCAG CTGGCTTCCC AGGGGCCCCA GGAATGCCTG 
GTCCGCGTCT ACATTATCCG GGCGTTTGGC CTGCAGCCCA AGGATCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCGGTGA GTGACCAGGA CAGCTACATC 
CCTTGTACTC TGGAGCCTGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC CCTCTATGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGGGAGACGG TCATTGACCT GGAGAACAGG CTGCTGTCTA AGTTCGGGGC CCGCTGTGGA 
CTCCCCCAGA CCTACTGCGT CTCTGGACCC AACCAGTGGA GGGATCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTGTTCTG CCAGCAGCAC CGAGTCAAGG CCCCCGTGTA CCGGACGGAC 
CGCGTGGTGT TTCAGGATAA AGAATACACC GTCGAAGAAA TAGAGGCTGG CAGGGTCCCG 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCCTTGC ACGTCCTCCA GCAGCAGGGG 
CTGATCCCTG AGCACGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC GGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCGGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAC 
ACCAAGGATG TGATCCTGGA TGACCTCAGC ATCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGGTTGG CTTTGAAGAA CACAAGCAAA AGACAGATGT ACATTATCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCGTCT TCCCTTTTGA CTACCTGCCA 
GCTGAGCAGG TCTGCACCGT CTCCAAGAAG GATGCATTCT GGAGGCTGGA CAAGACGGAG 
AGCAAGATCC CTGCACGAGT GGTATTCCAG ATCTGGGACA ACGACAAGTT CTCCTTCGAT 
GACTTTCTGG GCTCTCTGCA GCTGGACCTT AACCACATGC CCAAGCCCGC CAAGACAGCT 
GAGAAGTGCT CTGCGGACCA GCTGGAAGAC ACCTTCCACC CAGAGCGGTT TGTGTCTCTT 
TTTGAACAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTGG CGGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA GATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCAGCCG GCCACGGCCG GGATGAACCT AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGGCCTGACA CCTCCTTCCT CTGGTTCACC TCCCCGTACA AGACCATGAA GTTCATCCTG 
TGGCGGCGGT TCCGCTGTGC CATCATCTTC TTCCTCATTC TCTTCATCTT CCTGCTCTTC 
TTGGGCATCT TTGTCTACTC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015473399.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328885.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa91844
Clone ID Related Accession (Same CDS sequence)	XM_015473399.1
Accession Version	XM_015473399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT GCCAACGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGGAGGAG 
GAGTTCATTG ATTGGTGGAG CAAATTCTTT GCTTCAATTG GGGAGAGCGA AAAGTGCGGG 
TCCTACCTAG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACATCCCT GGAGAATGTG 
AAGGCCTTTG AGGGCCTGTC TGACTTTTGT AATACATTCA AGCTCTACCG GGGCAAGACA 
CAGGAGGAGA CTGAAGACCC ATCAGTCATT GGGGAGTTTA AGGGACTCTT CAAAATTTAT 
CCTCTCCCAG AAGACCCAGC CATCCCCTTG CCCCCGAGGC AGTTCCACCA GCTGGCTTCC 
CAGGGGCCCC AGGAATGCCT GGTCCGCGTC TACATTATCC GGGCGTTTGG CCTGCAGCCC 
AAGGATCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCGGTG 
AGTGACCAGG ACAGCTACAT CCCTTGTACT CTGGAGCCTG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CCCTCTATGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGGGAGACG GTCATTGACC TGGAGAACAG GCTGCTGTCT 
AAGTTCGGGG CCCGCTGTGG ACTCCCCCAG ACCTACTGCG TCTCTGGACC CAACCAGTGG 
AGGGATCAGC TCCGCCCCTC CCAGCTCCTC CACCTGTTCT GCCAGCAGCA CCGAGTCAAG 
GCCCCCGTGT ACCGGACGGA CCGCGTGGTG TTTCAGGATA AAGAATACAC CGTCGAAGAA 
ATAGAGGCTG GCAGGGTCCC GAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCAGGG GCTGATCCCT GAGCACGTGG AGTCCCGGCC TCTCTACAGC 
CCCCTGCAGC CGGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GGCCGGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACCAAGGAT GTGATCCTGG ATGACCTCAG CATCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TACATTATCG GTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCGTC 
TTCCCTTTTG ACTACCTGCC AGCTGAGCAG GTCTGCACCG TCTCCAAGAA GGATGCATTC 
TGGAGGCTGG ACAAGACGGA GAGCAAGATC CCTGCACGAG TGGTATTCCA GATCTGGGAC 
AACGACAAGT TCTCCTTCGA TGACTTTCTG GGCTCTCTGC AGCTGGACCT TAACCACATG 
CCCAAGCCCG CCAAGACAGC TGAGAAGTGC TCTGCGGACC AGCTGGAAGA CACCTTCCAC 
CCAGAGCGGT TTGTGTCTCT TTTTGAACAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTG 
GCGGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCAGCC GGCCACGGCC GGGATGAACC TAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGGCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGCTGTG CCATCATCTT CTTCCTCATT 
CTCTTCATCT TCCTGCTCTT CTTGGGCATC TTTGTCTACT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328885.1
CDS	130..6516
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEELCAPAFFLQGLLPTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015473399.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473399.1

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT GCCAACGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGGAGGAG 
GAGTTCATTG ATTGGTGGAG CAAATTCTTT GCTTCAATTG GGGAGAGCGA AAAGTGCGGG 
TCCTACCTAG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACATCCCT GGAGAATGTG 
AAGGCCTTTG AGGGCCTGTC TGACTTTTGT AATACATTCA AGCTCTACCG GGGCAAGACA 
CAGGAGGAGA CTGAAGACCC ATCAGTCATT GGGGAGTTTA AGGGACTCTT CAAAATTTAT 
CCTCTCCCAG AAGACCCAGC CATCCCCTTG CCCCCGAGGC AGTTCCACCA GCTGGCTTCC 
CAGGGGCCCC AGGAATGCCT GGTCCGCGTC TACATTATCC GGGCGTTTGG CCTGCAGCCC 
AAGGATCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCGGTG 
AGTGACCAGG ACAGCTACAT CCCTTGTACT CTGGAGCCTG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CCCTCTATGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGGGAGACG GTCATTGACC TGGAGAACAG GCTGCTGTCT 
AAGTTCGGGG CCCGCTGTGG ACTCCCCCAG ACCTACTGCG TCTCTGGACC CAACCAGTGG 
AGGGATCAGC TCCGCCCCTC CCAGCTCCTC CACCTGTTCT GCCAGCAGCA CCGAGTCAAG 
GCCCCCGTGT ACCGGACGGA CCGCGTGGTG TTTCAGGATA AAGAATACAC CGTCGAAGAA 
ATAGAGGCTG GCAGGGTCCC GAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCAGGG GCTGATCCCT GAGCACGTGG AGTCCCGGCC TCTCTACAGC 
CCCCTGCAGC CGGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GGCCGGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACCAAGGAT GTGATCCTGG ATGACCTCAG CATCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TACATTATCG GTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCGTC 
TTCCCTTTTG ACTACCTGCC AGCTGAGCAG GTCTGCACCG TCTCCAAGAA GGATGCATTC 
TGGAGGCTGG ACAAGACGGA GAGCAAGATC CCTGCACGAG TGGTATTCCA GATCTGGGAC 
AACGACAAGT TCTCCTTCGA TGACTTTCTG GGCTCTCTGC AGCTGGACCT TAACCACATG 
CCCAAGCCCG CCAAGACAGC TGAGAAGTGC TCTGCGGACC AGCTGGAAGA CACCTTCCAC 
CCAGAGCGGT TTGTGTCTCT TTTTGAACAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTG 
GCGGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCAGCC GGCCACGGCC GGGATGAACC TAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGGCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGCTGTG CCATCATCTT CTTCCTCATT 
CTCTTCATCT TCCTGCTCTT CTTGGGCATC TTTGTCTACT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001102490.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095960.1 Bos taurus dysferlin (DYSF), mRNA.

Price & Availability↑

CloneID	OBa01022
Clone ID Related Accession (Same CDS sequence)	NM_001102490.2
Accession Version	NM_001102490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	dysferlin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149938.1. On Aug 31, 2012 this sequence version replaced NM_001102490.1. ##Evidence-Data-START## Transcript exon combination :: BC149938.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGT GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGT CAGAGGACAC GGACCTGCCC TACCCGCCGC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CCGCCATCGA GATCCTGGCG 
TGGGGTCTTC GGAACATGAA GAGCTACCAG CTGGCCAGCG TGTCCTCCCC GAGCCTGGTG 
GTGGAGTGCG GGGGCCAGTC TGTACAATCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTGTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC CGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGGGCTTC CTGTGTGATC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GTGGCCCAGA TGACGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTGATT 
GACATCGATG ACAAGGAGCC CCTCATCCCC ATCCAGTTTG CAGATGGTCT CTCGGGCTTG 
GCCCCCACTA ACATGGCATC TTCTCCATCC AGTCTTCATA AGATCCTTCT GGAGGAGGAG 
TTCATTGATT GGTGGAGCAA ATTCTTTGCC TCAATTGGGG AGAGCGAAAA GTGCGGGTCC 
TACCTAGAGA AGGACTTTGA CACTCTAAAG GTCTATGACA CATCCCTGGA GAATGTGAAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAT ACATTCAAGC TCTACCGGGG CAAGACACAG 
GAGGAGACTG AAGACCCATC AGTCATTGGG GAGTTTAAGG GACTCTTCAA AATTTATCCT 
CTCCCAGAAG ACCCAGCCAT CCCCTTGCCC CCGAGGCAGT TCCACCAGCT GGCTTCCCAG 
GGGCCCCAGG AATGCCTGGT CCGCGTCTAC ATTATCCGGG CGTTTGGCCT GCAGCCCAAG 
GATCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCGGTGAGT 
GACCAGGACA GCTACATCCC TTGTACTCTG GAGCCTGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACCC TCTATGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG GGAGACGGTC ATTGACCTGG AGAACAGGCT GCTGTCTAAG 
TTCGGGGCCC GCTGTGGACT CCCCCAGACC TACTGCGTCT CTGGACCCAA CCAGTGGAGG 
GATCAGCTCC GCCCCTCCCA GCTCCTCCAC CTGTTCTGCC AGCAGCACCG AGTCAAGGCC 
CCCGTGTACC GGACGGACCG CGTGGTGTTT CAGGATAAAG AATACACCGT CGAAGAAATA 
GAGGCTGGCA GGGTCCCGAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCCTTGCAC 
GTCCTCCAGC AGCAGGGGCT GATCCCTGAG CACGTGGAGT CCCGGCCTCT CTACAGCCCC 
CTGCAGCCGG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CGGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAACAC CAAGGATGTG ATCCTGGATG ACCTCAGCAT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGGTTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGATGTAC ATTATCGGTC CCTGGGAGGT GAAGGCAACT TTAACTGGAG GTTCGTCTTC 
CCTTTTGACT ACCTGCCAGC TGAGCAGGTC TGCACCGTCT CCAAGAAGGA TGCATTCTGG 
AGGCTGGACA AGACGGAGAG CAAGATCCCT GCACGAGTGG TATTCCAGAT CTGGGACAAC 
GACAAGTTCT CCTTCGATGA CTTTCTGGGC TCTCTGCAGC TTGACCTTAA CCACATGCCC 
AAGCCCGCCA AGACAGCTGA GAAGTGCTCT GCGGACCAGC TGGAAGACAC CTTCCACCCA 
GAGCGGTTTG TGTCTCTTTT TGAACAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTGGCG 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAGA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCAGCCGGC CACGGCCGGG ATGAACCTAA CATGAACCCC 
AAGCTCGAGG ACCCAAGGCG GCCTGACACC TCCTTCCTCT GGTTCACCTC CCCGTACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGGTTC CGCTGTGCCA TCATCTTCTT CCTCATTCTC 
TTCATCTTCC TGCTCTTCTT GGGCATCTTT GTCTACTCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095960.1
CDS	462..6785
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDVDSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHKILLEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001102490.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin (DYSF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102490.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGT GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGT CAGAGGACAC GGACCTGCCC TACCCGCCGC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CCGCCATCGA GATCCTGGCG 
TGGGGTCTTC GGAACATGAA GAGCTACCAG CTGGCCAGCG TGTCCTCCCC GAGCCTGGTG 
GTGGAGTGCG GGGGCCAGTC TGTACAATCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTGTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC CGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGGGCTTC CTGTGTGATC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GTGGCCCAGA TGACGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTGATT 
GACATCGATG ACAAGGAGCC CCTCATCCCC ATCCAGTTTG CAGATGGTCT CTCGGGCTTG 
GCCCCCACTA ACATGGCATC TTCTCCATCC AGTCTTCATA AGATCCTTCT GGAGGAGGAG 
TTCATTGATT GGTGGAGCAA ATTCTTTGCC TCAATTGGGG AGAGCGAAAA GTGCGGGTCC 
TACCTAGAGA AGGACTTTGA CACTCTAAAG GTCTATGACA CATCCCTGGA GAATGTGAAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAT ACATTCAAGC TCTACCGGGG CAAGACACAG 
GAGGAGACTG AAGACCCATC AGTCATTGGG GAGTTTAAGG GACTCTTCAA AATTTATCCT 
CTCCCAGAAG ACCCAGCCAT CCCCTTGCCC CCGAGGCAGT TCCACCAGCT GGCTTCCCAG 
GGGCCCCAGG AATGCCTGGT CCGCGTCTAC ATTATCCGGG CGTTTGGCCT GCAGCCCAAG 
GATCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCGGTGAGT 
GACCAGGACA GCTACATCCC TTGTACTCTG GAGCCTGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACCC TCTATGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG GGAGACGGTC ATTGACCTGG AGAACAGGCT GCTGTCTAAG 
TTCGGGGCCC GCTGTGGACT CCCCCAGACC TACTGCGTCT CTGGACCCAA CCAGTGGAGG 
GATCAGCTCC GCCCCTCCCA GCTCCTCCAC CTGTTCTGCC AGCAGCACCG AGTCAAGGCC 
CCCGTGTACC GGACGGACCG CGTGGTGTTT CAGGATAAAG AATACACCGT CGAAGAAATA 
GAGGCTGGCA GGGTCCCGAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCCTTGCAC 
GTCCTCCAGC AGCAGGGGCT GATCCCTGAG CACGTGGAGT CCCGGCCTCT CTACAGCCCC 
CTGCAGCCGG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGGC CGGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAACAC CAAGGATGTG ATCCTGGATG ACCTCAGCAT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGGTTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGATGTAC ATTATCGGTC CCTGGGAGGT GAAGGCAACT TTAACTGGAG GTTCGTCTTC 
CCTTTTGACT ACCTGCCAGC TGAGCAGGTC TGCACCGTCT CCAAGAAGGA TGCATTCTGG 
AGGCTGGACA AGACGGAGAG CAAGATCCCT GCACGAGTGG TATTCCAGAT CTGGGACAAC 
GACAAGTTCT CCTTCGATGA CTTTCTGGGC TCTCTGCAGC TTGACCTTAA CCACATGCCC 
AAGCCCGCCA AGACAGCTGA GAAGTGCTCT GCGGACCAGC TGGAAGACAC CTTCCACCCA 
GAGCGGTTTG TGTCTCTTTT TGAACAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTGGCG 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAGA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCAGCCGGC CACGGCCGGG ATGAACCTAA CATGAACCCC 
AAGCTCGAGG ACCCAAGGCG GCCTGACACC TCCTTCCTCT GGTTCACCTC CCCGTACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGGTTC CGCTGTGCCA TCATCTTCTT CCTCATTCTC 
TTCATCTTCC TGCTCTTCTT GGGCATCTTT GTCTACTCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212484.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212541.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa23809
Clone ID Related Accession (Same CDS sequence)	XM_005212484.3
Accession Version	XM_005212484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGTGGACGAC CATCTAGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACATCA ACCTCTATGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA TCCCTATGCA 
GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTCCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCG CAGTGAACAG AAAGTGGAGG CTCTCTCTGC CGATGACATC 
CTTCGGGTAG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT CGATGACGCC GTCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACCAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGATG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCGTCTT ACTGGGAGGA CATCCGCCAC AGAGTTGAGG CTCAGAACCA GCTTCTCAGG 
ATTGCCGACC AGCTGGAAGC CGGCCTGGAG CAAGTCCACT TGGCCCTGAA GGCTCAGTGC 
TCCGACGAGG ACGCGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAACCCC TGGGTGATGT CCAGGAGATG CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGCCA CCTGAGCCAG ATCACCGAGG CGGCCCAGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCTG ACGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTTCAGGGG 
GACAAGCGGG TGGCGTACCA GCGGGTGCCT GCCCATGAGG TCCTCTTCTC CCGCCGGGGC 
ACCAGCTACT GTGGCAAGAA CTGTGGAAAA CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAAGGGGTGC CCAGAGCCCG GATGCCGGTG CAGATTCGGG TCAGGCTCTG GTTTGGACTC 
TCTGTGGATG AAAAGGAGTT CAACCAGTTT GCTGAGGGCA AGCTCTCCGT CTTCGCTGAA 
ACTTATGAGA ACCAGACCAA GCTGGCCCTG GTTGGGAACT GGGGCACAAC TGGCCTCACC 
TACCCCAAGT TTTCTGACAT CACGGGCAGG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC TGGAGACTGG TTCGTGTGTC CAGAGAAGAC ACTGCTTCAC 
GACACTGATG CTGGCCACCT GAGCTTCGTG GAGGAGGTAT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTGA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCGCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGGCGGCGG 
CGCTGGGTCC GTCTGCGCCG GAGGGACCTC AGCCAGATGG AGGCGTTGAA GCGGCACAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAT 
CTGGAGTACC GCAAGACAGA CGCCTTTCGT CGTCGCCGCT GGCGCCGCCG CATGGAGCCC 
TTGGAGAAGA CAGGGCCTGC GGCTGTGTTT GCCCTTGAGG GGGCCCTGGG TGGCGTGGTG 
GACGACAGGA GTGAAGATTC CGTGTCCGTC TCCACCTTGA GTTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGATC TGCCTGCAAT GGACAAGGAC TCTTTTTCCG ATCCCTATGC AGTCGTCTCC 
TTCCTGCACC AAAGCCAGAA GACGGTGGTG GCTAAGAACA CCCTAAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CAAGCAGCAT CGCCGAGCAG 
CCTCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ATGGTGTAGA TGAGTTCATG 
GGACGTTGCA TCTGTCAGCC GAGTCTGGAA CGGACGCCTC GGCTGGCCTG GTTCCCGCTG 
ACGAGGGGCA GCCAGCCCGC CGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCTGCCA TCCACCATAT TCCTGGTTTT GAGGTACAGG ACACAACAGG GATCCTGGAA 
GAGTCAGAGG ACACGGACCT GCCCTACCCA CCGCCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACCGCCA TCGAGATCCT GGCGTGGGGT 
CTTCGGAACA TGAAGAGCTA CCAGCTGGCC AGCGTGTCCT CCCCGAGCCT GGTGGTGGAG 
TGCGGGGGCC AGTCTGTACA ATCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTGTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCCGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGGG CTTCCTGTGT GATCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGATGACGT GAGCTTACTC AGTCCTGGGG AAGATGTGCT GATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATCCAG TTTGCAGATG GTCTCTCGGG CTTGGCCCCC 
ACTAACATGG CATCTTCTCC ATCCAGTCTT CATGAGGAGG AGTTCATTGA TTGGTGGAGC 
AAATTCTTTG CTTCAATTGG GGAGAGCGAA AAGTGCGGGT CCTACCTAGA GAAGGACTTT 
GACACTCTAA AGGTCTATGA CACATCCCTG GAGAATGTGA AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ATACATTCAA GCTCTACCGG GGCAAGACAC AGGAGGAGAC TGAAGACCCA 
TCAGTCATTG GGGAGTTTAA GGGACTCTTC AAAATTTATC CTCTCCCAGA AGACCCAGCC 
ATCCCCTTGC CCCCGAGGCA GTTCCACCAG CTGGCTTCCC AGGGGCCCCA GGAATGCCTG 
GTCCGCGTCT ACATTATCCG GGCGTTTGGC CTGCAGCCCA AGGATCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCGGTGA GTGACCAGGA CAGCTACATC 
CCTTGTACTC TGGAGCCTGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC CCTCTATGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGGGAGACGG TCATTGACCT GGAGAACAGG CTGCTGTCTA AGTTCGGGGC CCGCTGTGGA 
CTCCCCCAGA CCTACTGCGT CTCTGGACCC AACCAGTGGA GGGATCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTGTTCTG CCAGCAGCAC CGAGTCAAGG CCCCCGTGTA CCGGACGGAC 
CGCGTGGTGT TTCAGGATAA AGAATACACC GTCGAAGAAA TAGAGGCTGG CAGGGTCCCG 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCCTTGC ACGTCCTCCA GCAGCAGGGG 
CTGATCCCTG AGCACGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC GGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCGGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAC 
ACCAAGGATG TGATCCTGGA TGACCTCAGC ATCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGGTTGG CTTTGAAGAA CACAAGCAAA AGACAGATGT ACATTATCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCGTCT TCCCTTTTGA CTACCTGCCA 
GCTGAGCAGG TCTGCACCGT CTCCAAGAAG GATGCATTCT GGAGGCTGGA CAAGACGGAG 
AGCAAGATCC CTGCACGAGT GGTATTCCAG ATCTGGGACA ACGACAAGTT CTCCTTCGAT 
GACTTTCTGG GCTCTCTGCA GCTGGACCTT AACCACATGC CCAAGCCCGC CAAGACAGCT 
GAGAAGTGCT CTGCGGACCA GCTGGAAGAC ACCTTCCACC CAGAGCGGTT TGTGTCTCTT 
TTTGAACAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTGG CGGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA GATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCAGCCG GCCACGGCCG GGATGAACCT AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGGCCTGACA CCTCCTTCCT CTGGTTCACC TCCCCGTACA AGACCATGAA GTTCATCCTG 
TGGCGGCGGT TCCGCTGTGC CATCATCTTC TTCCTCATTC TCTTCATCTT CCTGCTCTTC 
TTGGGCATCT TTGTCTACTC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212541.1
CDS	130..6495
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212484.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212484.3

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGTGGACGAC CATCTAGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACATCA ACCTCTATGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA TCCCTATGCA 
GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTCCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCG CAGTGAACAG AAAGTGGAGG CTCTCTCTGC CGATGACATC 
CTTCGGGTAG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT CGATGACGCC GTCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACCAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGATG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCGTCTT ACTGGGAGGA CATCCGCCAC AGAGTTGAGG CTCAGAACCA GCTTCTCAGG 
ATTGCCGACC AGCTGGAAGC CGGCCTGGAG CAAGTCCACT TGGCCCTGAA GGCTCAGTGC 
TCCGACGAGG ACGCGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAACCCC TGGGTGATGT CCAGGAGATG CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGCCA CCTGAGCCAG ATCACCGAGG CGGCCCAGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCTG ACGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTTCAGGGG 
GACAAGCGGG TGGCGTACCA GCGGGTGCCT GCCCATGAGG TCCTCTTCTC CCGCCGGGGC 
ACCAGCTACT GTGGCAAGAA CTGTGGAAAA CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAAGGGGTGC CCAGAGCCCG GATGCCGGTG CAGATTCGGG TCAGGCTCTG GTTTGGACTC 
TCTGTGGATG AAAAGGAGTT CAACCAGTTT GCTGAGGGCA AGCTCTCCGT CTTCGCTGAA 
ACTTATGAGA ACCAGACCAA GCTGGCCCTG GTTGGGAACT GGGGCACAAC TGGCCTCACC 
TACCCCAAGT TTTCTGACAT CACGGGCAGG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC TGGAGACTGG TTCGTGTGTC CAGAGAAGAC ACTGCTTCAC 
GACACTGATG CTGGCCACCT GAGCTTCGTG GAGGAGGTAT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTGA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCGCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGGCGGCGG 
CGCTGGGTCC GTCTGCGCCG GAGGGACCTC AGCCAGATGG AGGCGTTGAA GCGGCACAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAT 
CTGGAGTACC GCAAGACAGA CGCCTTTCGT CGTCGCCGCT GGCGCCGCCG CATGGAGCCC 
TTGGAGAAGA CAGGGCCTGC GGCTGTGTTT GCCCTTGAGG GGGCCCTGGG TGGCGTGGTG 
GACGACAGGA GTGAAGATTC CGTGTCCGTC TCCACCTTGA GTTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGATC TGCCTGCAAT GGACAAGGAC TCTTTTTCCG ATCCCTATGC AGTCGTCTCC 
TTCCTGCACC AAAGCCAGAA GACGGTGGTG GCTAAGAACA CCCTAAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CAAGCAGCAT CGCCGAGCAG 
CCTCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ATGGTGTAGA TGAGTTCATG 
GGACGTTGCA TCTGTCAGCC GAGTCTGGAA CGGACGCCTC GGCTGGCCTG GTTCCCGCTG 
ACGAGGGGCA GCCAGCCCGC CGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCTGCCA TCCACCATAT TCCTGGTTTT GAGGTACAGG ACACAACAGG GATCCTGGAA 
GAGTCAGAGG ACACGGACCT GCCCTACCCA CCGCCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACCGCCA TCGAGATCCT GGCGTGGGGT 
CTTCGGAACA TGAAGAGCTA CCAGCTGGCC AGCGTGTCCT CCCCGAGCCT GGTGGTGGAG 
TGCGGGGGCC AGTCTGTACA ATCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTGTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCCGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGGG CTTCCTGTGT GATCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGATGACGT GAGCTTACTC AGTCCTGGGG AAGATGTGCT GATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATCCAG TTTGCAGATG GTCTCTCGGG CTTGGCCCCC 
ACTAACATGG CATCTTCTCC ATCCAGTCTT CATGAGGAGG AGTTCATTGA TTGGTGGAGC 
AAATTCTTTG CTTCAATTGG GGAGAGCGAA AAGTGCGGGT CCTACCTAGA GAAGGACTTT 
GACACTCTAA AGGTCTATGA CACATCCCTG GAGAATGTGA AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ATACATTCAA GCTCTACCGG GGCAAGACAC AGGAGGAGAC TGAAGACCCA 
TCAGTCATTG GGGAGTTTAA GGGACTCTTC AAAATTTATC CTCTCCCAGA AGACCCAGCC 
ATCCCCTTGC CCCCGAGGCA GTTCCACCAG CTGGCTTCCC AGGGGCCCCA GGAATGCCTG 
GTCCGCGTCT ACATTATCCG GGCGTTTGGC CTGCAGCCCA AGGATCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCGGTGA GTGACCAGGA CAGCTACATC 
CCTTGTACTC TGGAGCCTGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC CCTCTATGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGGGAGACGG TCATTGACCT GGAGAACAGG CTGCTGTCTA AGTTCGGGGC CCGCTGTGGA 
CTCCCCCAGA CCTACTGCGT CTCTGGACCC AACCAGTGGA GGGATCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTGTTCTG CCAGCAGCAC CGAGTCAAGG CCCCCGTGTA CCGGACGGAC 
CGCGTGGTGT TTCAGGATAA AGAATACACC GTCGAAGAAA TAGAGGCTGG CAGGGTCCCG 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCCTTGC ACGTCCTCCA GCAGCAGGGG 
CTGATCCCTG AGCACGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC GGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG GCCGGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAC 
ACCAAGGATG TGATCCTGGA TGACCTCAGC ATCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGGTTGG CTTTGAAGAA CACAAGCAAA AGACAGATGT ACATTATCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCGTCT TCCCTTTTGA CTACCTGCCA 
GCTGAGCAGG TCTGCACCGT CTCCAAGAAG GATGCATTCT GGAGGCTGGA CAAGACGGAG 
AGCAAGATCC CTGCACGAGT GGTATTCCAG ATCTGGGACA ACGACAAGTT CTCCTTCGAT 
GACTTTCTGG GCTCTCTGCA GCTGGACCTT AACCACATGC CCAAGCCCGC CAAGACAGCT 
GAGAAGTGCT CTGCGGACCA GCTGGAAGAC ACCTTCCACC CAGAGCGGTT TGTGTCTCTT 
TTTGAACAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTGG CGGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA GATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCAGCCG GCCACGGCCG GGATGAACCT AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGGCCTGACA CCTCCTTCCT CTGGTTCACC TCCCCGTACA AGACCATGAA GTTCATCCTG 
TGGCGGCGGT TCCGCTGTGC CATCATCTTC TTCCTCATTC TCTTCATCTT CCTGCTCTTC 
TTGGGCATCT TTGTCTACTC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212485.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212542.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa23810
Clone ID Related Accession (Same CDS sequence)	XM_005212485.3
Accession Version	XM_005212485.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGGTG GGGGCCAGAG CCTGGCCGAG 
ACCTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAC ACTCTGGAAG GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGACGAG 
GCCGAGCCAT TCCTGGACCA GAGCGGGGCT CTGGGCCCGG GGGCCCCCAG CACCCCGAAG 
AAACAACCTT CCCATCCGCC CCCCTACCAC CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT 
GCGCCCAGCA AGCCTCTGTC GGACAAGCCG CAGGACTTCC AGATCAGGGT CCAGGTGATC 
AAGGGACGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG 
CAGACCAAGC GGACGCGGAT CCACAAAGGA AACAGCCCCG TCTTCAATGA GACTCTCTTC 
TTCAACGTGT TTGACTCTCC CGCGGAGCTG TTCAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGAAC AGATGCTCTC ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC 
ATCTACCGAG AGCCCCGACA TGCCTACCTC AGGAAGTGGC TGCTGCTCTC AGATCCCGAC 
GATTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA 
GATGAAGCCC CCCTGGAGAG AAAAGACCCC TCTGAAGACA AGGAGGACAT CGAAAGCAAC 
CTGCTTAGGC CGATCGGCGT GGCCCTCCGT GGAGCGCACT TCTGTCTGAA GGTCTTCAGG 
GCCGAGGACT TGCCACAGAT GGACGATGCC GTGATGGACA ACGTGAGGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACG GCCAATCCTC AGTGGAACCA GAGCATCACA 
CTGCCTGCCA TGTTTCCATC CATGTGTGAG AAAATGAGGA TTCGTGTCGT AGACTGGGAC 
CGCCTCACCC ACAACGATAT TGTGGCCACC ACCTACCTGA GTATGTCGAA GATCTCTGCC 
TCTGGAGGCG AGATAGAAGT GGACGACCAT CTAGGCTTCC TCCCCACCTT CGGGCCCTGC 
TACATCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGATCC CTATGCAGAG 
CTCAACACGG GCAAGGGAGA AGGCGTGGCC TACCGAGGCC GGCTCCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCGCAG TGAACAGAAA GTGGAGGCTC TCTCTGCCGA TGACATCCTT 
CGGGTAGAGA AGTACCTCCG GAGGCGCAAG TACTCGCTCT TTGCCGCCTT CTACTCGGCC 
ACCATGCTGC AGGATGTCGA TGACGCCGTC CAGTTCGAGG TCAGCATTGG GAACTATGGC 
AACAAGTTCG ACACCACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGATGGAT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCGTCTTACT GGGAGGACAT CCGCCACAGA GTTGAGGCTC AGAACCAGCT TCTCAGGATT 
GCCGACCAGC TGGAAGCCGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCTCC 
GACGAGGACG CGGACTCCCT GGTGGCTCAG CTGATGGATG AGCTCATCGC AGGCTGCAGC 
CAACCCCTGG GTGATGTCCA GGAGATGCCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
CAGCTGCGCA CCCGCCACCT GAGCCAGATC ACCGAGGCGG CCCAGGCCCT GAAGCTCGGC 
CACAGTGAGC TCCCCGCTGC CCTGGAGCAG GCTGAGGACT GGCTCCTGCG GCTCCGTGCC 
CTGGCTGACG AGCCCCAGAA CAGCCTGCCT GACATCGTCA TCTGGATGCT TCAGGGGGAC 
AAGCGGGTGG CGTACCAGCG GGTGCCTGCC CATGAGGTCC TCTTCTCCCG CCGGGGCACC 
AGCTACTGTG GCAAGAACTG TGGAAAACTG CAGACGATAT TTCTGAAATA TCCGATGGAA 
GGGGTGCCCA GAGCCCGGAT GCCGGTGCAG ATTCGGGTCA GGCTCTGGTT TGGACTCTCT 
GTGGATGAAA AGGAGTTCAA CCAGTTTGCT GAGGGCAAGC TCTCCGTCTT CGCTGAAACT 
TATGAGAACC AGACCAAGCT GGCCCTGGTT GGGAACTGGG GCACAACTGG CCTCACCTAC 
CCCAAGTTTT CTGACATCAC GGGCAGGATC AAGCTACCCA AGGACAGCTT CCGCCCCTCA 
GCTGGCTGGG CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACACT GCTTCACGAC 
ACTGATGCTG GCCACCTGAG CTTCGTGGAG GAGGTATTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAACGGGGA GAAGGTGCTT 
CCCAAGGATG ACATCGAGTG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTGAATC GGGCTGTCGA TGAACAAGGC TGGGAGTACA GCATCACCAT CCCGCCGGAC 
CGGAAGCCGA GGCACTGGGT CCCTGCCGAG AAGATGTACT ACACACACCG GCGGCGGCGC 
TGGGTCCGTC TGCGCCGGAG GGACCTCAGC CAGATGGAGG CGTTGAAGCG GCACAGGCAG 
GCGGAGGCAG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TCGGCTGGAA GTTCCATCTG 
GAGTACCGCA AGACAGACGC CTTTCGTCGT CGCCGCTGGC GCCGCCGCAT GGAGCCCTTG 
GAGAAGACAG GGCCTGCGGC TGTGTTTGCC CTTGAGGGGG CCCTGGGTGG CGTGGTGGAC 
GACAGGAGTG AAGATTCCGT GTCCGTCTCC ACCTTGAGTT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC 
CGGGATCTGC CTGCAATGGA CAAGGACTCT TTTTCCGATC CCTATGCAGT CGTCTCCTTC 
CTGCACCAAA GCCAGAAGAC GGTGGTGGCT AAGAACACCC TAAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTACGAGAT CGAGATCTTT GGCGAGCCAA GCAGCATCGC CGAGCAGCCT 
CCCAGCATCG TGGTGGAGCT GTATGACCAT GACACCTATG GTGTAGATGA GTTCATGGGA 
CGTTGCATCT GTCAGCCGAG TCTGGAACGG ACGCCTCGGC TGGCCTGGTT CCCGCTGACG 
AGGGGCAGCC AGCCCGCCGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCTGCCATCC ACCATATTCC TGGTTTTGAG GTACAGGACA CAACAGGGAT CCTGGAAGAG 
TCAGAGGACA CGGACCTGCC CTACCCACCG CCCCAGAGGG AGGCCAACAT CTACATGGTC 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGA ACCGCCATCG AGATCCTGGC GTGGGGTCTT 
CGGAACATGA AGAGCTACCA GCTGGCCAGC GTGTCCTCCC CGAGCCTGGT GGTGGAGTGC 
GGGGGCCAGT CTGTACAATC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTGTACTG TCCCCCCATC 
GTGGTCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CCGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGGGCTT CCTGTGTGAT CCTTACTCAG AGGAGAGTCC GTCCCCACAG 
GGTGGCCCAG ATGACGTGAG CTTACTCAGT CCTGGGGAAG ATGTGCTGAT TGACATCGAT 
GACAAGGAGC CCCTCATCCC CATCCAGTTT GCAGATGGTC TCTCGGGCTT GGCCCCCACT 
AACATGGCAT CTTCTCCATC CAGTCTTCAT GAGGAGGAGT TCATTGATTG GTGGAGCAAA 
TTCTTTGCTT CAATTGGGGA GAGCGAAAAG TGCGGGTCCT ACCTAGAGAA GGACTTTGAC 
ACTCTAAAGG TCTATGACAC ATCCCTGGAG AATGTGAAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAATA CATTCAAGCT CTACCGGGGC AAGACACAGG AGGAGACTGA AGACCCATCA 
GTCATTGGGG AGTTTAAGGG ACTCTTCAAA ATTTATCCTC TCCCAGAAGA CCCAGCCATC 
CCCTTGCCCC CGAGGCAGTT CCACCAGCTG GCTTCCCAGG GGCCCCAGGA ATGCCTGGTC 
CGCGTCTACA TTATCCGGGC GTTTGGCCTG CAGCCCAAGG ATCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCGGTGAGTG ACCAGGACAG CTACATCCCT 
TGTACTCTGG AGCCTGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACCCT CTATGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGG 
GAGACGGTCA TTGACCTGGA GAACAGGCTG CTGTCTAAGT TCGGGGCCCG CTGTGGACTC 
CCCCAGACCT ACTGCGTCTC TGGACCCAAC CAGTGGAGGG ATCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TGTTCTGCCA GCAGCACCGA GTCAAGGCCC CCGTGTACCG GACGGACCGC 
GTGGTGTTTC AGGATAAAGA ATACACCGTC GAAGAAATAG AGGCTGGCAG GGTCCCGAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCCTTGCACG TCCTCCAGCA GCAGGGGCTG 
ATCCCTGAGC ACGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCGGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC GGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAACACC 
AAGGATGTGA TCCTGGATGA CCTCAGCATC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTACA TTATCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCGTCTTCC CTTTTGACTA CCTGCCAGCT 
GAGCAGGTCT GCACCGTCTC CAAGAAGGAT GCATTCTGGA GGCTGGACAA GACGGAGAGC 
AAGATCCCTG CACGAGTGGT ATTCCAGATC TGGGACAACG ACAAGTTCTC CTTCGATGAC 
TTTCTGGGCT CTCTGCAGCT GGACCTTAAC CACATGCCCA AGCCCGCCAA GACAGCTGAG 
AAGTGCTCTG CGGACCAGCT GGAAGACACC TTCCACCCAG AGCGGTTTGT GTCTCTTTTT 
GAACAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTGGCGG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAGAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCAGCCGGCC ACGGCCGGGA TGAACCTAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGG 
CCTGACACCT CCTTCCTCTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGGTTCC GCTGTGCCAT CATCTTCTTC CTCATTCTCT TCATCTTCCT GCTCTTCTTG 
GGCATCTTTG TCTACTCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212542.1
CDS	541..6903
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212485.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212485.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGGTG GGGGCCAGAG CCTGGCCGAG 
ACCTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAC ACTCTGGAAG GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGACGAG 
GCCGAGCCAT TCCTGGACCA GAGCGGGGCT CTGGGCCCGG GGGCCCCCAG CACCCCGAAG 
AAACAACCTT CCCATCCGCC CCCCTACCAC CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT 
GCGCCCAGCA AGCCTCTGTC GGACAAGCCG CAGGACTTCC AGATCAGGGT CCAGGTGATC 
AAGGGACGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG 
CAGACCAAGC GGACGCGGAT CCACAAAGGA AACAGCCCCG TCTTCAATGA GACTCTCTTC 
TTCAACGTGT TTGACTCTCC CGCGGAGCTG TTCAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGAAC AGATGCTCTC ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC 
ATCTACCGAG AGCCCCGACA TGCCTACCTC AGGAAGTGGC TGCTGCTCTC AGATCCCGAC 
GATTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA 
GATGAAGCCC CCCTGGAGAG AAAAGACCCC TCTGAAGACA AGGAGGACAT CGAAAGCAAC 
CTGCTTAGGC CGATCGGCGT GGCCCTCCGT GGAGCGCACT TCTGTCTGAA GGTCTTCAGG 
GCCGAGGACT TGCCACAGAT GGACGATGCC GTGATGGACA ACGTGAGGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACG GCCAATCCTC AGTGGAACCA GAGCATCACA 
CTGCCTGCCA TGTTTCCATC CATGTGTGAG AAAATGAGGA TTCGTGTCGT AGACTGGGAC 
CGCCTCACCC ACAACGATAT TGTGGCCACC ACCTACCTGA GTATGTCGAA GATCTCTGCC 
TCTGGAGGCG AGATAGAAGT GGACGACCAT CTAGGCTTCC TCCCCACCTT CGGGCCCTGC 
TACATCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGATCC CTATGCAGAG 
CTCAACACGG GCAAGGGAGA AGGCGTGGCC TACCGAGGCC GGCTCCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCGCAG TGAACAGAAA GTGGAGGCTC TCTCTGCCGA TGACATCCTT 
CGGGTAGAGA AGTACCTCCG GAGGCGCAAG TACTCGCTCT TTGCCGCCTT CTACTCGGCC 
ACCATGCTGC AGGATGTCGA TGACGCCGTC CAGTTCGAGG TCAGCATTGG GAACTATGGC 
AACAAGTTCG ACACCACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGATGGAT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCGTCTTACT GGGAGGACAT CCGCCACAGA GTTGAGGCTC AGAACCAGCT TCTCAGGATT 
GCCGACCAGC TGGAAGCCGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCTCC 
GACGAGGACG CGGACTCCCT GGTGGCTCAG CTGATGGATG AGCTCATCGC AGGCTGCAGC 
CAACCCCTGG GTGATGTCCA GGAGATGCCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
CAGCTGCGCA CCCGCCACCT GAGCCAGATC ACCGAGGCGG CCCAGGCCCT GAAGCTCGGC 
CACAGTGAGC TCCCCGCTGC CCTGGAGCAG GCTGAGGACT GGCTCCTGCG GCTCCGTGCC 
CTGGCTGACG AGCCCCAGAA CAGCCTGCCT GACATCGTCA TCTGGATGCT TCAGGGGGAC 
AAGCGGGTGG CGTACCAGCG GGTGCCTGCC CATGAGGTCC TCTTCTCCCG CCGGGGCACC 
AGCTACTGTG GCAAGAACTG TGGAAAACTG CAGACGATAT TTCTGAAATA TCCGATGGAA 
GGGGTGCCCA GAGCCCGGAT GCCGGTGCAG ATTCGGGTCA GGCTCTGGTT TGGACTCTCT 
GTGGATGAAA AGGAGTTCAA CCAGTTTGCT GAGGGCAAGC TCTCCGTCTT CGCTGAAACT 
TATGAGAACC AGACCAAGCT GGCCCTGGTT GGGAACTGGG GCACAACTGG CCTCACCTAC 
CCCAAGTTTT CTGACATCAC GGGCAGGATC AAGCTACCCA AGGACAGCTT CCGCCCCTCA 
GCTGGCTGGG CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACACT GCTTCACGAC 
ACTGATGCTG GCCACCTGAG CTTCGTGGAG GAGGTATTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAACGGGGA GAAGGTGCTT 
CCCAAGGATG ACATCGAGTG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTGAATC GGGCTGTCGA TGAACAAGGC TGGGAGTACA GCATCACCAT CCCGCCGGAC 
CGGAAGCCGA GGCACTGGGT CCCTGCCGAG AAGATGTACT ACACACACCG GCGGCGGCGC 
TGGGTCCGTC TGCGCCGGAG GGACCTCAGC CAGATGGAGG CGTTGAAGCG GCACAGGCAG 
GCGGAGGCAG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TCGGCTGGAA GTTCCATCTG 
GAGTACCGCA AGACAGACGC CTTTCGTCGT CGCCGCTGGC GCCGCCGCAT GGAGCCCTTG 
GAGAAGACAG GGCCTGCGGC TGTGTTTGCC CTTGAGGGGG CCCTGGGTGG CGTGGTGGAC 
GACAGGAGTG AAGATTCCGT GTCCGTCTCC ACCTTGAGTT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC 
CGGGATCTGC CTGCAATGGA CAAGGACTCT TTTTCCGATC CCTATGCAGT CGTCTCCTTC 
CTGCACCAAA GCCAGAAGAC GGTGGTGGCT AAGAACACCC TAAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTACGAGAT CGAGATCTTT GGCGAGCCAA GCAGCATCGC CGAGCAGCCT 
CCCAGCATCG TGGTGGAGCT GTATGACCAT GACACCTATG GTGTAGATGA GTTCATGGGA 
CGTTGCATCT GTCAGCCGAG TCTGGAACGG ACGCCTCGGC TGGCCTGGTT CCCGCTGACG 
AGGGGCAGCC AGCCCGCCGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCTGCCATCC ACCATATTCC TGGTTTTGAG GTACAGGACA CAACAGGGAT CCTGGAAGAG 
TCAGAGGACA CGGACCTGCC CTACCCACCG CCCCAGAGGG AGGCCAACAT CTACATGGTC 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGA ACCGCCATCG AGATCCTGGC GTGGGGTCTT 
CGGAACATGA AGAGCTACCA GCTGGCCAGC GTGTCCTCCC CGAGCCTGGT GGTGGAGTGC 
GGGGGCCAGT CTGTACAATC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTGTACTG TCCCCCCATC 
GTGGTCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CCGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGGGCTT CCTGTGTGAT CCTTACTCAG AGGAGAGTCC GTCCCCACAG 
GGTGGCCCAG ATGACGTGAG CTTACTCAGT CCTGGGGAAG ATGTGCTGAT TGACATCGAT 
GACAAGGAGC CCCTCATCCC CATCCAGTTT GCAGATGGTC TCTCGGGCTT GGCCCCCACT 
AACATGGCAT CTTCTCCATC CAGTCTTCAT GAGGAGGAGT TCATTGATTG GTGGAGCAAA 
TTCTTTGCTT CAATTGGGGA GAGCGAAAAG TGCGGGTCCT ACCTAGAGAA GGACTTTGAC 
ACTCTAAAGG TCTATGACAC ATCCCTGGAG AATGTGAAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAATA CATTCAAGCT CTACCGGGGC AAGACACAGG AGGAGACTGA AGACCCATCA 
GTCATTGGGG AGTTTAAGGG ACTCTTCAAA ATTTATCCTC TCCCAGAAGA CCCAGCCATC 
CCCTTGCCCC CGAGGCAGTT CCACCAGCTG GCTTCCCAGG GGCCCCAGGA ATGCCTGGTC 
CGCGTCTACA TTATCCGGGC GTTTGGCCTG CAGCCCAAGG ATCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCGGTGAGTG ACCAGGACAG CTACATCCCT 
TGTACTCTGG AGCCTGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACCCT CTATGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGG 
GAGACGGTCA TTGACCTGGA GAACAGGCTG CTGTCTAAGT TCGGGGCCCG CTGTGGACTC 
CCCCAGACCT ACTGCGTCTC TGGACCCAAC CAGTGGAGGG ATCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TGTTCTGCCA GCAGCACCGA GTCAAGGCCC CCGTGTACCG GACGGACCGC 
GTGGTGTTTC AGGATAAAGA ATACACCGTC GAAGAAATAG AGGCTGGCAG GGTCCCGAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCCTTGCACG TCCTCCAGCA GCAGGGGCTG 
ATCCCTGAGC ACGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCGGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC GGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAACACC 
AAGGATGTGA TCCTGGATGA CCTCAGCATC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTACA TTATCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCGTCTTCC CTTTTGACTA CCTGCCAGCT 
GAGCAGGTCT GCACCGTCTC CAAGAAGGAT GCATTCTGGA GGCTGGACAA GACGGAGAGC 
AAGATCCCTG CACGAGTGGT ATTCCAGATC TGGGACAACG ACAAGTTCTC CTTCGATGAC 
TTTCTGGGCT CTCTGCAGCT GGACCTTAAC CACATGCCCA AGCCCGCCAA GACAGCTGAG 
AAGTGCTCTG CGGACCAGCT GGAAGACACC TTCCACCCAG AGCGGTTTGT GTCTCTTTTT 
GAACAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTGGCGG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAGAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCAGCCGGCC ACGGCCGGGA TGAACCTAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGG 
CCTGACACCT CCTTCCTCTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGGTTCC GCTGTGCCAT CATCTTCTTC CTCATTCTCT TCATCTTCCT GCTCTTCTTG 
GGCATCTTTG TCTACTCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212487.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212544.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa23812
Clone ID Related Accession (Same CDS sequence)	XM_005212487.3
Accession Version	XM_005212487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGTCAGA GGACACGGAC 
CTGCCCTACC CACCGCCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACCGC CATCGAGATC CTGGCGTGGG GTCTTCGGAA CATGAAGAGC 
TACCAGCTGG CCAGCGTGTC CTCCCCGAGC CTGGTGGTGG AGTGCGGGGG CCAGTCTGTA 
CAATCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTG TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCCGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
GGCTTCCTGT GTGATCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATCC AGGAGGAGGA GTTCATTGAT TGGTGGAGCA AATTCTTTGC TTCAATTGGG 
GAGAGCGAAA AGTGCGGGTC CTACCTAGAG AAGGACTTTG ACACTCTAAA GGTCTATGAC 
ACATCCCTGG AGAATGTGAA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA TACATTCAAG 
CTCTACCGGG GCAAGACACA GGAGGAGACT GAAGACCCAT CAGTCATTGG GGAGTTTAAG 
GGACTCTTCA AAATTTATCC TCTCCCAGAA GACCCAGCCA TCCCCTTGCC CCCGAGGCAG 
TTCCACCAGC TGGCTTCCCA GGGGCCCCAG GAATGCCTGG TCCGCGTCTA CATTATCCGG 
GCGTTTGGCC TGCAGCCCAA GGATCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCGGTGAG TGACCAGGAC AGCTACATCC CTTGTACTCT GGAGCCTGTA 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGGTCACC 
CTCTATGACT ATGACCTCCT CTCCAAGGAT GAAAAGATTG GGGAGACGGT CATTGACCTG 
GAGAACAGGC TGCTGTCTAA GTTCGGGGCC CGCTGTGGAC TCCCCCAGAC CTACTGCGTC 
TCTGGACCCA ACCAGTGGAG GGATCAGCTC CGCCCCTCCC AGCTCCTCCA CCTGTTCTGC 
CAGCAGCACC GAGTCAAGGC CCCCGTGTAC CGGACGGACC GCGTGGTGTT TCAGGATAAA 
GAATACACCG TCGAAGAAAT AGAGGCTGGC AGGGTCCCGA ACCCACACCT GGGCCCAGTG 
GAGGAGCGTC TGGCCTTGCA CGTCCTCCAG CAGCAGGGGC TGATCCCTGA GCACGTGGAG 
TCCCGGCCTC TCTACAGCCC CCTGCAGCCG GACATCGAGC AGGGGAAGCT GCAGATGTGG 
GTCGACCTGT TTCCGAAGGC CCTGGGGCGG CCGGGACCTC CCTTCAACAT CACCCCACGG 
AGAGCCAGAA GGTTTTTCCT GCGTTGTATT ATCTGGAACA CCAAGGATGT GATCCTGGAT 
GACCTCAGCA TCACGGGGGA GAAGATGAGC GACATTTATG TGAAAGGTTG GATGGTTGGC 
TTTGAAGAAC ACAAGCAAAA GACAGATGTA CATTATCGGT CCCTGGGAGG TGAAGGCAAC 
TTTAACTGGA GGTTCGTCTT CCCTTTTGAC TACCTGCCAG CTGAGCAGGT CTGCACCGTC 
TCCAAGAAGG ATGCATTCTG GAGGCTGGAC AAGACGGAGA GCAAGATCCC TGCACGAGTG 
GTATTCCAGA TCTGGGACAA CGACAAGTTC TCCTTCGATG ACTTTCTGGG CTCTCTGCAG 
CTGGACCTTA ACCACATGCC CAAGCCCGCC AAGACAGCTG AGAAGTGCTC TGCGGACCAG 
CTGGAAGACA CCTTCCACCC AGAGCGGTTT GTGTCTCTTT TTGAACAGAA AACAGTGAAG 
GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT GAGAAGAAGA TACTGGCGGG CAAGCTGGAG 
ATGACCTTGG AGATTGTAAC AGAGAGTGAG CATGAGGAGC GGCCAGCCGG CCACGGCCGG 
GATGAACCTA ACATGAACCC CAAGCTCGAG GACCCAAGGC GGCCTGACAC CTCCTTCCTC 
TGGTTCACCT CCCCGTACAA GACCATGAAG TTCATCCTGT GGCGGCGGTT CCGCTGTGCC 
ATCATCTTCT TCCTCATTCT CTTCATCTTC CTGCTCTTCT TGGGCATCTT TGTCTACTCC 
TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212544.1
CDS	130..6474
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212487.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212487.3

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGTCAGA GGACACGGAC 
CTGCCCTACC CACCGCCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACCGC CATCGAGATC CTGGCGTGGG GTCTTCGGAA CATGAAGAGC 
TACCAGCTGG CCAGCGTGTC CTCCCCGAGC CTGGTGGTGG AGTGCGGGGG CCAGTCTGTA 
CAATCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTG TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCCGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
GGCTTCCTGT GTGATCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATCC AGGAGGAGGA GTTCATTGAT TGGTGGAGCA AATTCTTTGC TTCAATTGGG 
GAGAGCGAAA AGTGCGGGTC CTACCTAGAG AAGGACTTTG ACACTCTAAA GGTCTATGAC 
ACATCCCTGG AGAATGTGAA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA TACATTCAAG 
CTCTACCGGG GCAAGACACA GGAGGAGACT GAAGACCCAT CAGTCATTGG GGAGTTTAAG 
GGACTCTTCA AAATTTATCC TCTCCCAGAA GACCCAGCCA TCCCCTTGCC CCCGAGGCAG 
TTCCACCAGC TGGCTTCCCA GGGGCCCCAG GAATGCCTGG TCCGCGTCTA CATTATCCGG 
GCGTTTGGCC TGCAGCCCAA GGATCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCGGTGAG TGACCAGGAC AGCTACATCC CTTGTACTCT GGAGCCTGTA 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGGTCACC 
CTCTATGACT ATGACCTCCT CTCCAAGGAT GAAAAGATTG GGGAGACGGT CATTGACCTG 
GAGAACAGGC TGCTGTCTAA GTTCGGGGCC CGCTGTGGAC TCCCCCAGAC CTACTGCGTC 
TCTGGACCCA ACCAGTGGAG GGATCAGCTC CGCCCCTCCC AGCTCCTCCA CCTGTTCTGC 
CAGCAGCACC GAGTCAAGGC CCCCGTGTAC CGGACGGACC GCGTGGTGTT TCAGGATAAA 
GAATACACCG TCGAAGAAAT AGAGGCTGGC AGGGTCCCGA ACCCACACCT GGGCCCAGTG 
GAGGAGCGTC TGGCCTTGCA CGTCCTCCAG CAGCAGGGGC TGATCCCTGA GCACGTGGAG 
TCCCGGCCTC TCTACAGCCC CCTGCAGCCG GACATCGAGC AGGGGAAGCT GCAGATGTGG 
GTCGACCTGT TTCCGAAGGC CCTGGGGCGG CCGGGACCTC CCTTCAACAT CACCCCACGG 
AGAGCCAGAA GGTTTTTCCT GCGTTGTATT ATCTGGAACA CCAAGGATGT GATCCTGGAT 
GACCTCAGCA TCACGGGGGA GAAGATGAGC GACATTTATG TGAAAGGTTG GATGGTTGGC 
TTTGAAGAAC ACAAGCAAAA GACAGATGTA CATTATCGGT CCCTGGGAGG TGAAGGCAAC 
TTTAACTGGA GGTTCGTCTT CCCTTTTGAC TACCTGCCAG CTGAGCAGGT CTGCACCGTC 
TCCAAGAAGG ATGCATTCTG GAGGCTGGAC AAGACGGAGA GCAAGATCCC TGCACGAGTG 
GTATTCCAGA TCTGGGACAA CGACAAGTTC TCCTTCGATG ACTTTCTGGG CTCTCTGCAG 
CTGGACCTTA ACCACATGCC CAAGCCCGCC AAGACAGCTG AGAAGTGCTC TGCGGACCAG 
CTGGAAGACA CCTTCCACCC AGAGCGGTTT GTGTCTCTTT TTGAACAGAA AACAGTGAAG 
GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT GAGAAGAAGA TACTGGCGGG CAAGCTGGAG 
ATGACCTTGG AGATTGTAAC AGAGAGTGAG CATGAGGAGC GGCCAGCCGG CCACGGCCGG 
GATGAACCTA ACATGAACCC CAAGCTCGAG GACCCAAGGC GGCCTGACAC CTCCTTCCTC 
TGGTTCACCT CCCCGTACAA GACCATGAAG TTCATCCTGT GGCGGCGGTT CCGCTGTGCC 
ATCATCTTCT TCCTCATTCT CTTCATCTTC CTGCTCTTCT TGGGCATCTT TGTCTACTCC 
TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212488.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212545.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa23813
Clone ID Related Accession (Same CDS sequence)	XM_005212488.3
Accession Version	XM_005212488.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGGTG GGGGCCAGAG CCTGGCCGAG 
ACCTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAC ACTCTGGAAG GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGACGAG 
GCCGAGCCAT TCCTGGACCA GAGCGGGGCT CTGGGCCCGG GGGCCCCCAG CACCCCGAAG 
AAACAACCTT CCCATCCGCC CCCCTACCAC CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT 
GCGCCCAGCA AGCCTCTGTC GGACAAGCCG CAGGACTTCC AGATCAGGGT CCAGGTGATC 
AAGGGACGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG 
CAGACCAAGC GGACGCGGAT CCACAAAGGA AACAGCCCCG TCTTCAATGA GACTCTCTTC 
TTCAACGTGT TTGACTCTCC CGCGGAGCTG TTCAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGAAC AGATGCTCTC ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC 
ATCTACCGAG AGCCCCGACA TGCCTACCTC AGGAAGTGGC TGCTGCTCTC AGATCCCGAC 
GATTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA 
GATGAAGCCC CCCTGGAGAG AAAAGACCCC TCTGAAGACA AGGAGGACAT CGAAAGCAAC 
CTGCTTAGGC CGATCGGCGT GGCCCTCCGT GGAGCGCACT TCTGTCTGAA GGTCTTCAGG 
GCCGAGGACT TGCCACAGAT GGACGATGCC GTGATGGACA ACGTGAGGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACG GCCAATCCTC AGTGGAACCA GAGCATCACA 
CTGCCTGCCA TGTTTCCATC CATGTGTGAG AAAATGAGGA TTCGTGTCGT AGACTGGGAC 
CGCCTCACCC ACAACGATAT TGTGGCCACC ACCTACCTGA GTATGTCGAA GATCTCTGCC 
TCTGGAGGCG AGATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTCCTCC AGCCACAGAA 
CTGGACGACC ATCTAGGCTT CCTCCCCACC TTCGGGCCCT GCTACATCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAT CCCTATGCAG AGCTCAACAC GGGCAAGGGA 
GAAGGCGTGG CCTACCGAGG CCGGCTCCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCGC 
AGTGAACAGA AAGTGGAGGC TCTCTCTGCC GATGACATCC TTCGGGTAGA GAAGTACCTC 
CGGAGGCGCA AGTACTCGCT CTTTGCCGCC TTCTACTCGG CCACCATGCT GCAGGATGTC 
GATGACGCCG TCCAGTTCGA GGTCAGCATT GGGAACTATG GCAACAAGTT CGACACCACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC 
TACTACTTAC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC 
ATCCGCCACA GAGTTGAGGC TCAGAACCAG CTTCTCAGGA TTGCCGACCA GCTGGAAGCC 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCT CCGACGAGGA CGCGGACTCC 
CTGGTGGCTC AGCTGATGGA TGAGCTCATC GCAGGCTGCA GCCAACCCCT GGGTGATGTC 
CAGGAGATGC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACCAGCTGCG CACCCGCCAC 
CTGAGCCAGA TCACCGAGGC GGCCCAGGCC CTGAAGCTCG GCCACAGTGA GCTCCCCGCT 
GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG 
AACAGCCTGC CTGACATCGT CATCTGGATG CTTCAGGGGG ACAAGCGGGT GGCGTACCAG 
CGGGTGCCTG CCCATGAGGT CCTCTTCTCC CGCCGGGGCA CCAGCTACTG TGGCAAGAAC 
TGTGGAAAAC TGCAGACGAT ATTTCTGAAA TATCCGATGG AAGGGGTGCC CAGAGCCCGG 
ATGCCGGTGC AGATTCGGGT CAGGCTCTGG TTTGGACTCT CTGTGGATGA AAAGGAGTTC 
AACCAGTTTG CTGAGGGCAA GCTCTCCGTC TTCGCTGAAA CTTATGAGAA CCAGACCAAG 
CTGGCCCTGG TTGGGAACTG GGGCACAACT GGCCTCACCT ACCCCAAGTT TTCTGACATC 
ACGGGCAGGA TCAAGCTACC CAAGGACAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACA CTGCTTCACG ACACTGATGC TGGCCACCTG 
AGCTTCGTGG AGGAGGTATT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAG 
TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTGAA TCGGGCTGTC 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATCCCGCCGG ACCGGAAGCC GAGGCACTGG 
GTCCCTGCCG AGAAGATGTA CTACACACAC CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG 
AGGGACCTCA GCCAGATGGA GGCGTTGAAG CGGCACAGGC AGGCGGAGGC AGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG AAGTTCCATC TGGAGTACCG CAAGACAGAC 
GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG 
GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT GGCGTGGTGG ACGACAGGAG TGAAGATTCC 
GTGTCCGTCT CCACCTTGAG TTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT ACGCTGCTAC ATGTACCAGG CCCGGGATCT GCCTGCAATG 
GACAAGGACT CTTTTTCCGA TCCCTATGCA GTCGTCTCCT TCCTGCACCA AAGCCAGAAG 
ACGGTGGTGG CTAAGAACAC CCTAAACCCC ACCTGGGACC AGACGCTCAT CTTCTACGAG 
ATCGAGATCT TTGGCGAGCC AAGCAGCATC GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGTGTAGAT GAGTTCATGG GACGTTGCAT CTGTCAGCCG 
AGTCTGGAAC GGACGCCTCG GCTGGCCTGG TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCTGCCAT CCACCATATT 
CCTGGTTTTG AGGTACAGGA CACAACAGGG ATCCTGGAAG AGTCAGAGGA CACGGACCTG 
CCCTACCCAC CGCCCCAGAG GGAGGCCAAC ATCTACATGG TCCCCCAGAA CATCAAGCCG 
GTTCTCCAGC GAACCGCCAT CGAGATCCTG GCGTGGGGTC TTCGGAACAT GAAGAGCTAC 
CAGCTGGCCA GCGTGTCCTC CCCGAGCCTG GTGGTGGAGT GCGGGGGCCA GTCTGTACAA 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTCG ACATCTGCAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTGTAC TGTCCCCCCA TCGTGGTCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCCGTGGTG GGCCAGTGTA CCATCCGCTC CCTGGAGGGC 
TTCCTGTGTG ATCCTTACTC AGAGGAGAGT CCGTCCCCAC AGGGTGGCCC AGATGACGTG 
AGCTTACTCA GTCCTGGGGA AGATGTGCTG ATTGACATCG ATGACAAGGA GCCCCTCATC 
CCCATCCAGG AGGAGGAGTT CATTGATTGG TGGAGCAAAT TCTTTGCTTC AATTGGGGAG 
AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG GACTTTGACA CTCTAAAGGT CTATGACACA 
TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC CTGTCTGACT TTTGTAATAC ATTCAAGCTC 
TACCGGGGCA AGACACAGGA GGAGACTGAA GACCCATCAG TCATTGGGGA GTTTAAGGGA 
CTCTTCAAAA TTTATCCTCT CCCAGAAGAC CCAGCCATCC CCTTGCCCCC GAGGCAGTTC 
CACCAGCTGG CTTCCCAGGG GCCCCAGGAA TGCCTGGTCC GCGTCTACAT TATCCGGGCG 
TTTGGCCTGC AGCCCAAGGA TCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CGGTGAGTGA CCAGGACAGC TACATCCCTT GTACTCTGGA GCCTGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACCCTC 
TATGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGGG AGACGGTCAT TGACCTGGAG 
AACAGGCTGC TGTCTAAGTT CGGGGCCCGC TGTGGACTCC CCCAGACCTA CTGCGTCTCT 
GGACCCAACC AGTGGAGGGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT GTTCTGCCAG 
CAGCACCGAG TCAAGGCCCC CGTGTACCGG ACGGACCGCG TGGTGTTTCA GGATAAAGAA 
TACACCGTCG AAGAAATAGA GGCTGGCAGG GTCCCGAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CCTTGCACGT CCTCCAGCAG CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC 
CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCG GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAACACCA AGGATGTGAT CCTGGATGAC 
CTCAGCATCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTACAT TATCGGTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCGTCTTCCC TTTTGACTAC CTGCCAGCTG AGCAGGTCTG CACCGTCTCC 
AAGAAGGATG CATTCTGGAG GCTGGACAAG ACGGAGAGCA AGATCCCTGC ACGAGTGGTA 
TTCCAGATCT GGGACAACGA CAAGTTCTCC TTCGATGACT TTCTGGGCTC TCTGCAGCTG 
GACCTTAACC ACATGCCCAA GCCCGCCAAG ACAGCTGAGA AGTGCTCTGC GGACCAGCTG 
GAAGACACCT TCCACCCAGA GCGGTTTGTG TCTCTTTTTG AACAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAGATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT 
GAACCTAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGGC CTGACACCTC CTTCCTCTGG 
TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCCTGTGGC GGCGGTTCCG CTGTGCCATC 
ATCTTCTTCC TCATTCTCTT CATCTTCCTG CTCTTCTTGG GCATCTTTGT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212545.1
CDS	540..6881
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212488.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212488.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGGTG GGGGCCAGAG CCTGGCCGAG 
ACCTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAC ACTCTGGAAG GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGACGAG 
GCCGAGCCAT TCCTGGACCA GAGCGGGGCT CTGGGCCCGG GGGCCCCCAG CACCCCGAAG 
AAACAACCTT CCCATCCGCC CCCCTACCAC CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT 
GCGCCCAGCA AGCCTCTGTC GGACAAGCCG CAGGACTTCC AGATCAGGGT CCAGGTGATC 
AAGGGACGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG 
CAGACCAAGC GGACGCGGAT CCACAAAGGA AACAGCCCCG TCTTCAATGA GACTCTCTTC 
TTCAACGTGT TTGACTCTCC CGCGGAGCTG TTCAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGAAC AGATGCTCTC ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC 
ATCTACCGAG AGCCCCGACA TGCCTACCTC AGGAAGTGGC TGCTGCTCTC AGATCCCGAC 
GATTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA 
GATGAAGCCC CCCTGGAGAG AAAAGACCCC TCTGAAGACA AGGAGGACAT CGAAAGCAAC 
CTGCTTAGGC CGATCGGCGT GGCCCTCCGT GGAGCGCACT TCTGTCTGAA GGTCTTCAGG 
GCCGAGGACT TGCCACAGAT GGACGATGCC GTGATGGACA ACGTGAGGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACG GCCAATCCTC AGTGGAACCA GAGCATCACA 
CTGCCTGCCA TGTTTCCATC CATGTGTGAG AAAATGAGGA TTCGTGTCGT AGACTGGGAC 
CGCCTCACCC ACAACGATAT TGTGGCCACC ACCTACCTGA GTATGTCGAA GATCTCTGCC 
TCTGGAGGCG AGATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTCCTCC AGCCACAGAA 
CTGGACGACC ATCTAGGCTT CCTCCCCACC TTCGGGCCCT GCTACATCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAT CCCTATGCAG AGCTCAACAC GGGCAAGGGA 
GAAGGCGTGG CCTACCGAGG CCGGCTCCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCGC 
AGTGAACAGA AAGTGGAGGC TCTCTCTGCC GATGACATCC TTCGGGTAGA GAAGTACCTC 
CGGAGGCGCA AGTACTCGCT CTTTGCCGCC TTCTACTCGG CCACCATGCT GCAGGATGTC 
GATGACGCCG TCCAGTTCGA GGTCAGCATT GGGAACTATG GCAACAAGTT CGACACCACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC 
TACTACTTAC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC 
ATCCGCCACA GAGTTGAGGC TCAGAACCAG CTTCTCAGGA TTGCCGACCA GCTGGAAGCC 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCT CCGACGAGGA CGCGGACTCC 
CTGGTGGCTC AGCTGATGGA TGAGCTCATC GCAGGCTGCA GCCAACCCCT GGGTGATGTC 
CAGGAGATGC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACCAGCTGCG CACCCGCCAC 
CTGAGCCAGA TCACCGAGGC GGCCCAGGCC CTGAAGCTCG GCCACAGTGA GCTCCCCGCT 
GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG 
AACAGCCTGC CTGACATCGT CATCTGGATG CTTCAGGGGG ACAAGCGGGT GGCGTACCAG 
CGGGTGCCTG CCCATGAGGT CCTCTTCTCC CGCCGGGGCA CCAGCTACTG TGGCAAGAAC 
TGTGGAAAAC TGCAGACGAT ATTTCTGAAA TATCCGATGG AAGGGGTGCC CAGAGCCCGG 
ATGCCGGTGC AGATTCGGGT CAGGCTCTGG TTTGGACTCT CTGTGGATGA AAAGGAGTTC 
AACCAGTTTG CTGAGGGCAA GCTCTCCGTC TTCGCTGAAA CTTATGAGAA CCAGACCAAG 
CTGGCCCTGG TTGGGAACTG GGGCACAACT GGCCTCACCT ACCCCAAGTT TTCTGACATC 
ACGGGCAGGA TCAAGCTACC CAAGGACAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACA CTGCTTCACG ACACTGATGC TGGCCACCTG 
AGCTTCGTGG AGGAGGTATT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAG 
TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTGAA TCGGGCTGTC 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATCCCGCCGG ACCGGAAGCC GAGGCACTGG 
GTCCCTGCCG AGAAGATGTA CTACACACAC CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG 
AGGGACCTCA GCCAGATGGA GGCGTTGAAG CGGCACAGGC AGGCGGAGGC AGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG AAGTTCCATC TGGAGTACCG CAAGACAGAC 
GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG 
GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT GGCGTGGTGG ACGACAGGAG TGAAGATTCC 
GTGTCCGTCT CCACCTTGAG TTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT ACGCTGCTAC ATGTACCAGG CCCGGGATCT GCCTGCAATG 
GACAAGGACT CTTTTTCCGA TCCCTATGCA GTCGTCTCCT TCCTGCACCA AAGCCAGAAG 
ACGGTGGTGG CTAAGAACAC CCTAAACCCC ACCTGGGACC AGACGCTCAT CTTCTACGAG 
ATCGAGATCT TTGGCGAGCC AAGCAGCATC GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGTGTAGAT GAGTTCATGG GACGTTGCAT CTGTCAGCCG 
AGTCTGGAAC GGACGCCTCG GCTGGCCTGG TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCTGCCAT CCACCATATT 
CCTGGTTTTG AGGTACAGGA CACAACAGGG ATCCTGGAAG AGTCAGAGGA CACGGACCTG 
CCCTACCCAC CGCCCCAGAG GGAGGCCAAC ATCTACATGG TCCCCCAGAA CATCAAGCCG 
GTTCTCCAGC GAACCGCCAT CGAGATCCTG GCGTGGGGTC TTCGGAACAT GAAGAGCTAC 
CAGCTGGCCA GCGTGTCCTC CCCGAGCCTG GTGGTGGAGT GCGGGGGCCA GTCTGTACAA 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTCG ACATCTGCAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTGTAC TGTCCCCCCA TCGTGGTCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCCGTGGTG GGCCAGTGTA CCATCCGCTC CCTGGAGGGC 
TTCCTGTGTG ATCCTTACTC AGAGGAGAGT CCGTCCCCAC AGGGTGGCCC AGATGACGTG 
AGCTTACTCA GTCCTGGGGA AGATGTGCTG ATTGACATCG ATGACAAGGA GCCCCTCATC 
CCCATCCAGG AGGAGGAGTT CATTGATTGG TGGAGCAAAT TCTTTGCTTC AATTGGGGAG 
AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG GACTTTGACA CTCTAAAGGT CTATGACACA 
TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC CTGTCTGACT TTTGTAATAC ATTCAAGCTC 
TACCGGGGCA AGACACAGGA GGAGACTGAA GACCCATCAG TCATTGGGGA GTTTAAGGGA 
CTCTTCAAAA TTTATCCTCT CCCAGAAGAC CCAGCCATCC CCTTGCCCCC GAGGCAGTTC 
CACCAGCTGG CTTCCCAGGG GCCCCAGGAA TGCCTGGTCC GCGTCTACAT TATCCGGGCG 
TTTGGCCTGC AGCCCAAGGA TCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CGGTGAGTGA CCAGGACAGC TACATCCCTT GTACTCTGGA GCCTGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACCCTC 
TATGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGGG AGACGGTCAT TGACCTGGAG 
AACAGGCTGC TGTCTAAGTT CGGGGCCCGC TGTGGACTCC CCCAGACCTA CTGCGTCTCT 
GGACCCAACC AGTGGAGGGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT GTTCTGCCAG 
CAGCACCGAG TCAAGGCCCC CGTGTACCGG ACGGACCGCG TGGTGTTTCA GGATAAAGAA 
TACACCGTCG AAGAAATAGA GGCTGGCAGG GTCCCGAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CCTTGCACGT CCTCCAGCAG CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC 
CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGGCCG GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAACACCA AGGATGTGAT CCTGGATGAC 
CTCAGCATCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTACAT TATCGGTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCGTCTTCCC TTTTGACTAC CTGCCAGCTG AGCAGGTCTG CACCGTCTCC 
AAGAAGGATG CATTCTGGAG GCTGGACAAG ACGGAGAGCA AGATCCCTGC ACGAGTGGTA 
TTCCAGATCT GGGACAACGA CAAGTTCTCC TTCGATGACT TTCTGGGCTC TCTGCAGCTG 
GACCTTAACC ACATGCCCAA GCCCGCCAAG ACAGCTGAGA AGTGCTCTGC GGACCAGCTG 
GAAGACACCT TCCACCCAGA GCGGTTTGTG TCTCTTTTTG AACAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAGATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT 
GAACCTAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGGC CTGACACCTC CTTCCTCTGG 
TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCCTGTGGC GGCGGTTCCG CTGTGCCATC 
ATCTTCTTCC TCATTCTCTT CATCTTCCTG CTCTTCTTGG GCATCTTTGT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212489.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212546.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa23814
Clone ID Related Accession (Same CDS sequence)	XM_005212489.3
Accession Version	XM_005212489.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212489.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTCCTCC AGCCACAGAA CTGGACGACC ATCTAGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACATCAA CCTCTATGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAT 
CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTCCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCGC AGTGAACAGA AAGTGGAGGC TCTCTCTGCC 
GATGACATCC TTCGGGTAGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT GCAGGATGTC GATGACGCCG TCCAGTTCGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACCACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGATGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC ATCCGCCACA GAGTTGAGGC TCAGAACCAG 
CTTCTCAGGA TTGCCGACCA GCTGGAAGCC GGCCTGGAGC AAGTCCACTT GGCCCTGAAG 
GCTCAGTGCT CCGACGAGGA CGCGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATC 
GCAGGCTGCA GCCAACCCCT GGGTGATGTC CAGGAGATGC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGT ACCAGCTGCG CACCCGCCAC CTGAGCCAGA TCACCGAGGC GGCCCAGGCC 
CTGAAGCTCG GCCACAGTGA GCTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTTCAGGGGG ACAAGCGGGT GGCGTACCAG CGGGTGCCTG CCCATGAGGT CCTCTTCTCC 
CGCCGGGGCA CCAGCTACTG TGGCAAGAAC TGTGGAAAAC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AAGGGGTGCC CAGAGCCCGG ATGCCGGTGC AGATTCGGGT CAGGCTCTGG 
TTTGGACTCT CTGTGGATGA AAAGGAGTTC AACCAGTTTG CTGAGGGCAA GCTCTCCGTC 
TTCGCTGAAA CTTATGAGAA CCAGACCAAG CTGGCCCTGG TTGGGAACTG GGGCACAACT 
GGCCTCACCT ACCCCAAGTT TTCTGACATC ACGGGCAGGA TCAAGCTACC CAAGGACAGC 
TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT GGAGACTGGT TCGTGTGTCC AGAGAAGACA 
CTGCTTCACG ACACTGATGC TGGCCACCTG AGCTTCGTGG AGGAGGTATT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTGAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCGCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC 
CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG AGGGACCTCA GCCAGATGGA GGCGTTGAAG 
CGGCACAGGC AGGCGGAGGC AGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG 
AAGTTCCATC TGGAGTACCG CAAGACAGAC GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC 
ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT 
GGCGTGGTGG ACGACAGGAG TGAAGATTCC GTGTCCGTCT CCACCTTGAG TTTTGGTGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT ACGCTGCTAC 
ATGTACCAGG CCCGGGATCT GCCTGCAATG GACAAGGACT CTTTTTCCGA TCCCTATGCA 
GTCGTCTCCT TCCTGCACCA AAGCCAGAAG ACGGTGGTGG CTAAGAACAC CCTAAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTACGAG ATCGAGATCT TTGGCGAGCC AAGCAGCATC 
GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA TGGTGTAGAT 
GAGTTCATGG GACGTTGCAT CTGTCAGCCG AGTCTGGAAC GGACGCCTCG GCTGGCCTGG 
TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCTGCCAT CCACCATATT CCTGGTTTTG AGGTACAGGA CACAACAGGG 
ATCCTGGAAG AGTCAGAGGA CACGGACCTG CCCTACCCAC CGCCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACCGCCAT CGAGATCCTG 
GCGTGGGGTC TTCGGAACAT GAAGAGCTAC CAGCTGGCCA GCGTGTCCTC CCCGAGCCTG 
GTGGTGGAGT GCGGGGGCCA GTCTGTACAA TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTGTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCCGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGGGC TTCCTGTGTG ATCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGTGGCCC AGATGACGTG AGCTTACTCA GTCCTGGGGA AGATGTGCTG 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATCCAGT TTGCAGATGG TCTCTCGGGC 
TTGGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCTTC ATGAGGAGGA GTTCATTGAT 
TGGTGGAGCA AATTCTTTGC TTCAATTGGG GAGAGCGAAA AGTGCGGGTC CTACCTAGAG 
AAGGACTTTG ACACTCTAAA GGTCTATGAC ACATCCCTGG AGAATGTGAA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA TACATTCAAG CTCTACCGGG GCAAGACACA GGAGGAGACT 
GAAGACCCAT CAGTCATTGG GGAGTTTAAG GGACTCTTCA AAATTTATCC TCTCCCAGAA 
GACCCAGCCA TCCCCTTGCC CCCGAGGCAG TTCCACCAGC TGGCTTCCCA GGGGCCCCAG 
GAATGCCTGG TCCGCGTCTA CATTATCCGG GCGTTTGGCC TGCAGCCCAA GGATCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCGGTGAG TGACCAGGAC 
AGCTACATCC CTTGTACTCT GGAGCCTGTA TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGGTCACC CTCTATGACT ATGACCTCCT CTCCAAGGAT 
GAAAAGATTG GGGAGACGGT CATTGACCTG GAGAACAGGC TGCTGTCTAA GTTCGGGGCC 
CGCTGTGGAC TCCCCCAGAC CTACTGCGTC TCTGGACCCA ACCAGTGGAG GGATCAGCTC 
CGCCCCTCCC AGCTCCTCCA CCTGTTCTGC CAGCAGCACC GAGTCAAGGC CCCCGTGTAC 
CGGACGGACC GCGTGGTGTT TCAGGATAAA GAATACACCG TCGAAGAAAT AGAGGCTGGC 
AGGGTCCCGA ACCCACACCT GGGCCCAGTG GAGGAGCGTC TGGCCTTGCA CGTCCTCCAG 
CAGCAGGGGC TGATCCCTGA GCACGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCG 
GACATCGAGC AGGGGAAGCT GCAGATGTGG GTCGACCTGT TTCCGAAGGC CCTGGGGCGG 
CCGGGACCTC CCTTCAACAT CACCCCACGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATT 
ATCTGGAACA CCAAGGATGT GATCCTGGAT GACCTCAGCA TCACGGGGGA GAAGATGAGC 
GACATTTATG TGAAAGGTTG GATGGTTGGC TTTGAAGAAC ACAAGCAAAA GACAGATGTA 
CATTATCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCGTCTT CCCTTTTGAC 
TACCTGCCAG CTGAGCAGGT CTGCACCGTC TCCAAGAAGG ATGCATTCTG GAGGCTGGAC 
AAGACGGAGA GCAAGATCCC TGCACGAGTG GTATTCCAGA TCTGGGACAA CGACAAGTTC 
TCCTTCGATG ACTTTCTGGG CTCTCTGCAG CTGGACCTTA ACCACATGCC CAAGCCCGCC 
AAGACAGCTG AGAAGTGCTC TGCGGACCAG CTGGAAGACA CCTTCCACCC AGAGCGGTTT 
GTGTCTCTTT TTGAACAGAA AACAGTGAAG GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT 
GAGAAGAAGA TACTGGCGGG CAAGCTGGAG ATGACCTTGG AGATTGTAAC AGAGAGTGAG 
CATGAGGAGC GGCCAGCCGG CCACGGCCGG GATGAACCTA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GGCCTGACAC CTCCTTCCTC TGGTTCACCT CCCCGTACAA GACCATGAAG 
TTCATCCTGT GGCGGCGGTT CCGCTGTGCC ATCATCTTCT TCCTCATTCT CTTCATCTTC 
CTGCTCTTCT TGGGCATCTT TGTCTACTCC TTCCCGAACT ATGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212546.1
CDS	130..6444
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212489.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212489.3

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTCCTCC AGCCACAGAA CTGGACGACC ATCTAGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACATCAA CCTCTATGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAT 
CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTCCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCGC AGTGAACAGA AAGTGGAGGC TCTCTCTGCC 
GATGACATCC TTCGGGTAGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT GCAGGATGTC GATGACGCCG TCCAGTTCGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACCACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGATGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC ATCCGCCACA GAGTTGAGGC TCAGAACCAG 
CTTCTCAGGA TTGCCGACCA GCTGGAAGCC GGCCTGGAGC AAGTCCACTT GGCCCTGAAG 
GCTCAGTGCT CCGACGAGGA CGCGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATC 
GCAGGCTGCA GCCAACCCCT GGGTGATGTC CAGGAGATGC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGT ACCAGCTGCG CACCCGCCAC CTGAGCCAGA TCACCGAGGC GGCCCAGGCC 
CTGAAGCTCG GCCACAGTGA GCTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTTCAGGGGG ACAAGCGGGT GGCGTACCAG CGGGTGCCTG CCCATGAGGT CCTCTTCTCC 
CGCCGGGGCA CCAGCTACTG TGGCAAGAAC TGTGGAAAAC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AAGGGGTGCC CAGAGCCCGG ATGCCGGTGC AGATTCGGGT CAGGCTCTGG 
TTTGGACTCT CTGTGGATGA AAAGGAGTTC AACCAGTTTG CTGAGGGCAA GCTCTCCGTC 
TTCGCTGAAA CTTATGAGAA CCAGACCAAG CTGGCCCTGG TTGGGAACTG GGGCACAACT 
GGCCTCACCT ACCCCAAGTT TTCTGACATC ACGGGCAGGA TCAAGCTACC CAAGGACAGC 
TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT GGAGACTGGT TCGTGTGTCC AGAGAAGACA 
CTGCTTCACG ACACTGATGC TGGCCACCTG AGCTTCGTGG AGGAGGTATT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTGAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCGCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC 
CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG AGGGACCTCA GCCAGATGGA GGCGTTGAAG 
CGGCACAGGC AGGCGGAGGC AGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG 
AAGTTCCATC TGGAGTACCG CAAGACAGAC GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC 
ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT 
GGCGTGGTGG ACGACAGGAG TGAAGATTCC GTGTCCGTCT CCACCTTGAG TTTTGGTGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT ACGCTGCTAC 
ATGTACCAGG CCCGGGATCT GCCTGCAATG GACAAGGACT CTTTTTCCGA TCCCTATGCA 
GTCGTCTCCT TCCTGCACCA AAGCCAGAAG ACGGTGGTGG CTAAGAACAC CCTAAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTACGAG ATCGAGATCT TTGGCGAGCC AAGCAGCATC 
GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA TGGTGTAGAT 
GAGTTCATGG GACGTTGCAT CTGTCAGCCG AGTCTGGAAC GGACGCCTCG GCTGGCCTGG 
TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCTGCCAT CCACCATATT CCTGGTTTTG AGGTACAGGA CACAACAGGG 
ATCCTGGAAG AGTCAGAGGA CACGGACCTG CCCTACCCAC CGCCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACCGCCAT CGAGATCCTG 
GCGTGGGGTC TTCGGAACAT GAAGAGCTAC CAGCTGGCCA GCGTGTCCTC CCCGAGCCTG 
GTGGTGGAGT GCGGGGGCCA GTCTGTACAA TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTGTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCCGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGGGC TTCCTGTGTG ATCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGTGGCCC AGATGACGTG AGCTTACTCA GTCCTGGGGA AGATGTGCTG 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATCCAGT TTGCAGATGG TCTCTCGGGC 
TTGGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCTTC ATGAGGAGGA GTTCATTGAT 
TGGTGGAGCA AATTCTTTGC TTCAATTGGG GAGAGCGAAA AGTGCGGGTC CTACCTAGAG 
AAGGACTTTG ACACTCTAAA GGTCTATGAC ACATCCCTGG AGAATGTGAA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA TACATTCAAG CTCTACCGGG GCAAGACACA GGAGGAGACT 
GAAGACCCAT CAGTCATTGG GGAGTTTAAG GGACTCTTCA AAATTTATCC TCTCCCAGAA 
GACCCAGCCA TCCCCTTGCC CCCGAGGCAG TTCCACCAGC TGGCTTCCCA GGGGCCCCAG 
GAATGCCTGG TCCGCGTCTA CATTATCCGG GCGTTTGGCC TGCAGCCCAA GGATCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCGGTGAG TGACCAGGAC 
AGCTACATCC CTTGTACTCT GGAGCCTGTA TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGGTCACC CTCTATGACT ATGACCTCCT CTCCAAGGAT 
GAAAAGATTG GGGAGACGGT CATTGACCTG GAGAACAGGC TGCTGTCTAA GTTCGGGGCC 
CGCTGTGGAC TCCCCCAGAC CTACTGCGTC TCTGGACCCA ACCAGTGGAG GGATCAGCTC 
CGCCCCTCCC AGCTCCTCCA CCTGTTCTGC CAGCAGCACC GAGTCAAGGC CCCCGTGTAC 
CGGACGGACC GCGTGGTGTT TCAGGATAAA GAATACACCG TCGAAGAAAT AGAGGCTGGC 
AGGGTCCCGA ACCCACACCT GGGCCCAGTG GAGGAGCGTC TGGCCTTGCA CGTCCTCCAG 
CAGCAGGGGC TGATCCCTGA GCACGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCG 
GACATCGAGC AGGGGAAGCT GCAGATGTGG GTCGACCTGT TTCCGAAGGC CCTGGGGCGG 
CCGGGACCTC CCTTCAACAT CACCCCACGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATT 
ATCTGGAACA CCAAGGATGT GATCCTGGAT GACCTCAGCA TCACGGGGGA GAAGATGAGC 
GACATTTATG TGAAAGGTTG GATGGTTGGC TTTGAAGAAC ACAAGCAAAA GACAGATGTA 
CATTATCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCGTCTT CCCTTTTGAC 
TACCTGCCAG CTGAGCAGGT CTGCACCGTC TCCAAGAAGG ATGCATTCTG GAGGCTGGAC 
AAGACGGAGA GCAAGATCCC TGCACGAGTG GTATTCCAGA TCTGGGACAA CGACAAGTTC 
TCCTTCGATG ACTTTCTGGG CTCTCTGCAG CTGGACCTTA ACCACATGCC CAAGCCCGCC 
AAGACAGCTG AGAAGTGCTC TGCGGACCAG CTGGAAGACA CCTTCCACCC AGAGCGGTTT 
GTGTCTCTTT TTGAACAGAA AACAGTGAAG GGCTGGTGGC CCTGTGTGGC GGAAGAGGGT 
GAGAAGAAGA TACTGGCGGG CAAGCTGGAG ATGACCTTGG AGATTGTAAC AGAGAGTGAG 
CATGAGGAGC GGCCAGCCGG CCACGGCCGG GATGAACCTA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GGCCTGACAC CTCCTTCCTC TGGTTCACCT CCCCGTACAA GACCATGAAG 
TTCATCCTGT GGCGGCGGTT CCGCTGTGCC ATCATCTTCT TCCTCATTCT CTTCATCTTC 
CTGCTCTTCT TGGGCATCTT TGTCTACTCC TTCCCGAACT ATGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212490.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212547.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa23815
Clone ID Related Accession (Same CDS sequence)	XM_005212490.3
Accession Version	XM_005212490.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGT CAGAGGACAC GGACCTGCCC TACCCACCGC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CCGCCATCGA GATCCTGGCG 
TGGGGTCTTC GGAACATGAA GAGCTACCAG CTGGCCAGCG TGTCCTCCCC GAGCCTGGTG 
GTGGAGTGCG GGGGCCAGTC TGTACAATCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTGTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC CGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGGGCTTC CTGTGTGATC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GTGGCCCAGA TGACGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTGATT 
GACATCGATG ACAAGGAGCC CCTCATCCCC ATCCAGTTTG CAGATGGTCT CTCGGGCTTG 
GCCCCCACTA ACATGGCATC TTCTCCATCC AGTCTTCATG AGGAGGAGTT CATTGATTGG 
TGGAGCAAAT TCTTTGCTTC AATTGGGGAG AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG 
GACTTTGACA CTCTAAAGGT CTATGACACA TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAATAC ATTCAAGCTC TACCGGGGCA AGACACAGGA GGAGACTGAA 
GACCCATCAG TCATTGGGGA GTTTAAGGGA CTCTTCAAAA TTTATCCTCT CCCAGAAGAC 
CCAGCCATCC CCTTGCCCCC GAGGCAGTTC CACCAGCTGG CTTCCCAGGG GCCCCAGGAA 
TGCCTGGTCC GCGTCTACAT TATCCGGGCG TTTGGCCTGC AGCCCAAGGA TCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CGGTGAGTGA CCAGGACAGC 
TACATCCCTT GTACTCTGGA GCCTGTATTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GGTCACCCTC TATGACTATG ACCTCCTCTC CAAGGATGAA 
AAGATTGGGG AGACGGTCAT TGACCTGGAG AACAGGCTGC TGTCTAAGTT CGGGGCCCGC 
TGTGGACTCC CCCAGACCTA CTGCGTCTCT GGACCCAACC AGTGGAGGGA TCAGCTCCGC 
CCCTCCCAGC TCCTCCACCT GTTCTGCCAG CAGCACCGAG TCAAGGCCCC CGTGTACCGG 
ACGGACCGCG TGGTGTTTCA GGATAAAGAA TACACCGTCG AAGAAATAGA GGCTGGCAGG 
GTCCCGAACC CACACCTGGG CCCAGTGGAG GAGCGTCTGG CCTTGCACGT CCTCCAGCAG 
CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC 
ATCGAGCAGG GGAAGCTGCA GATGTGGGTC GACCTGTTTC CGAAGGCCCT GGGGCGGCCG 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACCA AGGATGTGAT CCTGGATGAC CTCAGCATCA CGGGGGAGAA GATGAGCGAC 
ATTTATGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTACAT 
TATCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCGTCTTCCC TTTTGACTAC 
CTGCCAGCTG AGCAGGTCTG CACCGTCTCC AAGAAGGATG CATTCTGGAG GCTGGACAAG 
ACGGAGAGCA AGATCCCTGC ACGAGTGGTA TTCCAGATCT GGGACAACGA CAAGTTCTCC 
TTCGATGACT TTCTGGGCTC TCTGCAGCTG GACCTTAACC ACATGCCCAA GCCCGCCAAG 
ACAGCTGAGA AGTGCTCTGC GGACCAGCTG GAAGACACCT TCCACCCAGA GCGGTTTGTG 
TCTCTTTTTG AACAGAAAAC AGTGAAGGGC TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG 
AAGAAGATAC TGGCGGGCAA GCTGGAGATG ACCTTGGAGA TTGTAACAGA GAGTGAGCAT 
GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT GAACCTAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGGC CTGACACCTC CTTCCTCTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG CTGTGCCATC ATCTTCTTCC TCATTCTCTT CATCTTCCTG 
CTCTTCTTGG GCATCTTTGT CTACTCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212547.1
CDS	540..6851
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212490.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212490.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGT CAGAGGACAC GGACCTGCCC TACCCACCGC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CCGCCATCGA GATCCTGGCG 
TGGGGTCTTC GGAACATGAA GAGCTACCAG CTGGCCAGCG TGTCCTCCCC GAGCCTGGTG 
GTGGAGTGCG GGGGCCAGTC TGTACAATCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTGTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC CGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGGGCTTC CTGTGTGATC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GTGGCCCAGA TGACGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTGATT 
GACATCGATG ACAAGGAGCC CCTCATCCCC ATCCAGTTTG CAGATGGTCT CTCGGGCTTG 
GCCCCCACTA ACATGGCATC TTCTCCATCC AGTCTTCATG AGGAGGAGTT CATTGATTGG 
TGGAGCAAAT TCTTTGCTTC AATTGGGGAG AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG 
GACTTTGACA CTCTAAAGGT CTATGACACA TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAATAC ATTCAAGCTC TACCGGGGCA AGACACAGGA GGAGACTGAA 
GACCCATCAG TCATTGGGGA GTTTAAGGGA CTCTTCAAAA TTTATCCTCT CCCAGAAGAC 
CCAGCCATCC CCTTGCCCCC GAGGCAGTTC CACCAGCTGG CTTCCCAGGG GCCCCAGGAA 
TGCCTGGTCC GCGTCTACAT TATCCGGGCG TTTGGCCTGC AGCCCAAGGA TCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CGGTGAGTGA CCAGGACAGC 
TACATCCCTT GTACTCTGGA GCCTGTATTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GGTCACCCTC TATGACTATG ACCTCCTCTC CAAGGATGAA 
AAGATTGGGG AGACGGTCAT TGACCTGGAG AACAGGCTGC TGTCTAAGTT CGGGGCCCGC 
TGTGGACTCC CCCAGACCTA CTGCGTCTCT GGACCCAACC AGTGGAGGGA TCAGCTCCGC 
CCCTCCCAGC TCCTCCACCT GTTCTGCCAG CAGCACCGAG TCAAGGCCCC CGTGTACCGG 
ACGGACCGCG TGGTGTTTCA GGATAAAGAA TACACCGTCG AAGAAATAGA GGCTGGCAGG 
GTCCCGAACC CACACCTGGG CCCAGTGGAG GAGCGTCTGG CCTTGCACGT CCTCCAGCAG 
CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC 
ATCGAGCAGG GGAAGCTGCA GATGTGGGTC GACCTGTTTC CGAAGGCCCT GGGGCGGCCG 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACCA AGGATGTGAT CCTGGATGAC CTCAGCATCA CGGGGGAGAA GATGAGCGAC 
ATTTATGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTACAT 
TATCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCGTCTTCCC TTTTGACTAC 
CTGCCAGCTG AGCAGGTCTG CACCGTCTCC AAGAAGGATG CATTCTGGAG GCTGGACAAG 
ACGGAGAGCA AGATCCCTGC ACGAGTGGTA TTCCAGATCT GGGACAACGA CAAGTTCTCC 
TTCGATGACT TTCTGGGCTC TCTGCAGCTG GACCTTAACC ACATGCCCAA GCCCGCCAAG 
ACAGCTGAGA AGTGCTCTGC GGACCAGCTG GAAGACACCT TCCACCCAGA GCGGTTTGTG 
TCTCTTTTTG AACAGAAAAC AGTGAAGGGC TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG 
AAGAAGATAC TGGCGGGCAA GCTGGAGATG ACCTTGGAGA TTGTAACAGA GAGTGAGCAT 
GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT GAACCTAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGGC CTGACACCTC CTTCCTCTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG CTGTGCCATC ATCTTCTTCC TCATTCTCTT CATCTTCCTG 
CTCTTCTTGG GCATCTTTGT CTACTCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212491.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212548.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa23816
Clone ID Related Accession (Same CDS sequence)	XM_005212491.3
Accession Version	XM_005212491.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212491.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGTGGACGAC CATCTAGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACATCA ACCTCTATGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA TCCCTATGCA 
GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTCCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCG CAGTGAACAG AAAGTGGAGG CTCTCTCTGC CGATGACATC 
CTTCGGGTAG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT CGATGACGCC GTCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACCAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGATG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCGTCTT ACTGGGAGGA CATCCGCCAC AGAGTTGAGG CTCAGAACCA GCTTCTCAGG 
ATTGCCGACC AGCTGGAAGC CGGCCTGGAG CAAGTCCACT TGGCCCTGAA GGCTCAGTGC 
TCCGACGAGG ACGCGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAACCCC TGGGTGATGT CCAGGAGATG CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGCCA CCTGAGCCAG ATCACCGAGG CGGCCCAGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCTG ACGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTTCAGGGG 
GACAAGCGGG TGGCGTACCA GCGGGTGCCT GCCCATGAGG TCCTCTTCTC CCGCCGGGGC 
ACCAGCTACT GTGGCAAGAA CTGTGGAAAA CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAAGGGGTGC CCAGAGCCCG GATGCCGGTG CAGATTCGGG TCAGGCTCTG GTTTGGACTC 
TCTGTGGATG AAAAGGAGTT CAACCAGTTT GCTGAGGGCA AGCTCTCCGT CTTCGCTGAA 
ACTTATGAGA ACCAGACCAA GCTGGCCCTG GTTGGGAACT GGGGCACAAC TGGCCTCACC 
TACCCCAAGT TTTCTGACAT CACGGGCAGG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC TGGAGACTGG TTCGTGTGTC CAGAGAAGAC ACTGCTTCAC 
GACACTGATG CTGGCCACCT GAGCTTCGTG GAGGAGGTAT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTGA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCGCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGGCGGCGG 
CGCTGGGTCC GTCTGCGCCG GAGGGACCTC AGCCAGATGG AGGCGTTGAA GCGGCACAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAT 
CTGGAGTACC GCAAGACAGA CGCCTTTCGT CGTCGCCGCT GGCGCCGCCG CATGGAGCCC 
TTGGAGAAGA CAGGGCCTGC GGCTGTGTTT GCCCTTGAGG GGGCCCTGGG TGGCGTGGTG 
GACGACAGGA GTGAAGATTC CGTGTCCGTC TCCACCTTGA GTTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGATC TGCCTGCAAT GGACAAGGAC TCTTTTTCCG ATCCCTATGC AGTCGTCTCC 
TTCCTGCACC AAAGCCAGAA GACGGTGGTG GCTAAGAACA CCCTAAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CAAGCAGCAT CGCCGAGCAG 
CCTCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ATGGTGTAGA TGAGTTCATG 
GGACGTTGCA TCTGTCAGCC GAGTCTGGAA CGGACGCCTC GGCTGGCCTG GTTCCCGCTG 
ACGAGGGGCA GCCAGCCCGC CGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCTGCCA TCCACCATAT TCCTGGTTTT GAGGTACAGG ACACAACAGG GATCCTGGAA 
GAGTCAGAGG ACACGGACCT GCCCTACCCA CCGCCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACCGCCA TCGAGATCCT GGCGTGGGGT 
CTTCGGAACA TGAAGAGCTA CCAGCTGGCC AGCGTGTCCT CCCCGAGCCT GGTGGTGGAG 
TGCGGGGGCC AGTCTGTACA ATCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTGTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCCGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGGG CTTCCTGTGT GATCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGATGACGT GAGCTTACTC AGTCCTGGGG AAGATGTGCT GATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAGGAGT TCATTGATTG GTGGAGCAAA 
TTCTTTGCTT CAATTGGGGA GAGCGAAAAG TGCGGGTCCT ACCTAGAGAA GGACTTTGAC 
ACTCTAAAGG TCTATGACAC ATCCCTGGAG AATGTGAAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAATA CATTCAAGCT CTACCGGGGC AAGACACAGG AGGAGACTGA AGACCCATCA 
GTCATTGGGG AGTTTAAGGG ACTCTTCAAA ATTTATCCTC TCCCAGAAGA CCCAGCCATC 
CCCTTGCCCC CGAGGCAGTT CCACCAGCTG GCTTCCCAGG GGCCCCAGGA ATGCCTGGTC 
CGCGTCTACA TTATCCGGGC GTTTGGCCTG CAGCCCAAGG ATCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCGGTGAGTG ACCAGGACAG CTACATCCCT 
TGTACTCTGG AGCCTGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACCCT CTATGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGG 
GAGACGGTCA TTGACCTGGA GAACAGGCTG CTGTCTAAGT TCGGGGCCCG CTGTGGACTC 
CCCCAGACCT ACTGCGTCTC TGGACCCAAC CAGTGGAGGG ATCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TGTTCTGCCA GCAGCACCGA GTCAAGGCCC CCGTGTACCG GACGGACCGC 
GTGGTGTTTC AGGATAAAGA ATACACCGTC GAAGAAATAG AGGCTGGCAG GGTCCCGAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCCTTGCACG TCCTCCAGCA GCAGGGGCTG 
ATCCCTGAGC ACGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCGGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC GGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAACACC 
AAGGATGTGA TCCTGGATGA CCTCAGCATC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTACA TTATCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCGTCTTCC CTTTTGACTA CCTGCCAGCT 
GAGCAGGTCT GCACCGTCTC CAAGAAGGAT GCATTCTGGA GGCTGGACAA GACGGAGAGC 
AAGATCCCTG CACGAGTGGT ATTCCAGATC TGGGACAACG ACAAGTTCTC CTTCGATGAC 
TTTCTGGGCT CTCTGCAGCT GGACCTTAAC CACATGCCCA AGCCCGCCAA GACAGCTGAG 
AAGTGCTCTG CGGACCAGCT GGAAGACACC TTCCACCCAG AGCGGTTTGT GTCTCTTTTT 
GAACAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTGGCGG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAGAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCAGCCGGCC ACGGCCGGGA TGAACCTAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGG 
CCTGACACCT CCTTCCTCTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGGTTCC GCTGTGCCAT CATCTTCTTC CTCATTCTCT TCATCTTCCT GCTCTTCTTG 
GGCATCTTTG TCTACTCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212548.1
CDS	129..6431
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212491.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212491.3

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGTGGACGAC CATCTAGGCT TCCTCCCCAC CTTCGGGCCC 
TGCTACATCA ACCTCTATGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA TCCCTATGCA 
GAGCTCAACA CGGGCAAGGG AGAAGGCGTG GCCTACCGAG GCCGGCTCCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCG CAGTGAACAG AAAGTGGAGG CTCTCTCTGC CGATGACATC 
CTTCGGGTAG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT CGATGACGCC GTCCAGTTCG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACCAC CTGCCTGCCG CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGATG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCGTCTT ACTGGGAGGA CATCCGCCAC AGAGTTGAGG CTCAGAACCA GCTTCTCAGG 
ATTGCCGACC AGCTGGAAGC CGGCCTGGAG CAAGTCCACT TGGCCCTGAA GGCTCAGTGC 
TCCGACGAGG ACGCGGACTC CCTGGTGGCT CAGCTGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAACCCC TGGGTGATGT CCAGGAGATG CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGCCA CCTGAGCCAG ATCACCGAGG CGGCCCAGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGGCTCCGT 
GCCCTGGCTG ACGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTTCAGGGG 
GACAAGCGGG TGGCGTACCA GCGGGTGCCT GCCCATGAGG TCCTCTTCTC CCGCCGGGGC 
ACCAGCTACT GTGGCAAGAA CTGTGGAAAA CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAAGGGGTGC CCAGAGCCCG GATGCCGGTG CAGATTCGGG TCAGGCTCTG GTTTGGACTC 
TCTGTGGATG AAAAGGAGTT CAACCAGTTT GCTGAGGGCA AGCTCTCCGT CTTCGCTGAA 
ACTTATGAGA ACCAGACCAA GCTGGCCCTG GTTGGGAACT GGGGCACAAC TGGCCTCACC 
TACCCCAAGT TTTCTGACAT CACGGGCAGG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC TGGAGACTGG TTCGTGTGTC CAGAGAAGAC ACTGCTTCAC 
GACACTGATG CTGGCCACCT GAGCTTCGTG GAGGAGGTAT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA GTGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTGA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCGCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCC GAGAAGATGT ACTACACACA CCGGCGGCGG 
CGCTGGGTCC GTCTGCGCCG GAGGGACCTC AGCCAGATGG AGGCGTTGAA GCGGCACAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCGC TCTTCGGCTG GAAGTTCCAT 
CTGGAGTACC GCAAGACAGA CGCCTTTCGT CGTCGCCGCT GGCGCCGCCG CATGGAGCCC 
TTGGAGAAGA CAGGGCCTGC GGCTGTGTTT GCCCTTGAGG GGGCCCTGGG TGGCGTGGTG 
GACGACAGGA GTGAAGATTC CGTGTCCGTC TCCACCTTGA GTTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGATC TGCCTGCAAT GGACAAGGAC TCTTTTTCCG ATCCCTATGC AGTCGTCTCC 
TTCCTGCACC AAAGCCAGAA GACGGTGGTG GCTAAGAACA CCCTAAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CAAGCAGCAT CGCCGAGCAG 
CCTCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ATGGTGTAGA TGAGTTCATG 
GGACGTTGCA TCTGTCAGCC GAGTCTGGAA CGGACGCCTC GGCTGGCCTG GTTCCCGCTG 
ACGAGGGGCA GCCAGCCCGC CGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCTGCCA TCCACCATAT TCCTGGTTTT GAGGTACAGG ACACAACAGG GATCCTGGAA 
GAGTCAGAGG ACACGGACCT GCCCTACCCA CCGCCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACCGCCA TCGAGATCCT GGCGTGGGGT 
CTTCGGAACA TGAAGAGCTA CCAGCTGGCC AGCGTGTCCT CCCCGAGCCT GGTGGTGGAG 
TGCGGGGGCC AGTCTGTACA ATCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTGTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCCGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGGG CTTCCTGTGT GATCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGTGGCC CAGATGACGT GAGCTTACTC AGTCCTGGGG AAGATGTGCT GATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAGGAGT TCATTGATTG GTGGAGCAAA 
TTCTTTGCTT CAATTGGGGA GAGCGAAAAG TGCGGGTCCT ACCTAGAGAA GGACTTTGAC 
ACTCTAAAGG TCTATGACAC ATCCCTGGAG AATGTGAAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAATA CATTCAAGCT CTACCGGGGC AAGACACAGG AGGAGACTGA AGACCCATCA 
GTCATTGGGG AGTTTAAGGG ACTCTTCAAA ATTTATCCTC TCCCAGAAGA CCCAGCCATC 
CCCTTGCCCC CGAGGCAGTT CCACCAGCTG GCTTCCCAGG GGCCCCAGGA ATGCCTGGTC 
CGCGTCTACA TTATCCGGGC GTTTGGCCTG CAGCCCAAGG ATCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCGGTGAGTG ACCAGGACAG CTACATCCCT 
TGTACTCTGG AGCCTGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACCCT CTATGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGG 
GAGACGGTCA TTGACCTGGA GAACAGGCTG CTGTCTAAGT TCGGGGCCCG CTGTGGACTC 
CCCCAGACCT ACTGCGTCTC TGGACCCAAC CAGTGGAGGG ATCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TGTTCTGCCA GCAGCACCGA GTCAAGGCCC CCGTGTACCG GACGGACCGC 
GTGGTGTTTC AGGATAAAGA ATACACCGTC GAAGAAATAG AGGCTGGCAG GGTCCCGAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCCTTGCACG TCCTCCAGCA GCAGGGGCTG 
ATCCCTGAGC ACGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCGGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGGCC GGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAACACC 
AAGGATGTGA TCCTGGATGA CCTCAGCATC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTACA TTATCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCGTCTTCC CTTTTGACTA CCTGCCAGCT 
GAGCAGGTCT GCACCGTCTC CAAGAAGGAT GCATTCTGGA GGCTGGACAA GACGGAGAGC 
AAGATCCCTG CACGAGTGGT ATTCCAGATC TGGGACAACG ACAAGTTCTC CTTCGATGAC 
TTTCTGGGCT CTCTGCAGCT GGACCTTAAC CACATGCCCA AGCCCGCCAA GACAGCTGAG 
AAGTGCTCTG CGGACCAGCT GGAAGACACC TTCCACCCAG AGCGGTTTGT GTCTCTTTTT 
GAACAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTGGCGG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAGAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCAGCCGGCC ACGGCCGGGA TGAACCTAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGG 
CCTGACACCT CCTTCCTCTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGGTTCC GCTGTGCCAT CATCTTCTTC CTCATTCTCT TCATCTTCCT GCTCTTCTTG 
GGCATCTTTG TCTACTCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212492.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212549.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa23817
Clone ID Related Accession (Same CDS sequence)	XM_005212492.3
Accession Version	XM_005212492.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212492.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGGTG GGGGCCAGAG CCTGGCCGAG 
ACCTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAC ACTCTGGAAG GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGACGAG 
GCCGAGCCAT TCCTGGACCA GAGCGGGGCT CTGGGCCCGG GGGCCCCCAG CACCCCGAAG 
AAACAACCTT CCCATCCGCC CCCCTACCAC CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT 
GCGCCCAGCA AGCCTCTGTC GGACAAGCCG CAGGACTTCC AGATCAGGGT CCAGGTGATC 
AAGGGACGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG 
CAGACCAAGC GGACGCGGAT CCACAAAGGA AACAGCCCCG TCTTCAATGA GACTCTCTTC 
TTCAACGTGT TTGACTCTCC CGCGGAGCTG TTCAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGAAC AGATGCTCTC ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC 
ATCTACCGAG AGCCCCGACA TGCCTACCTC AGGAAGTGGC TGCTGCTCTC AGATCCCGAC 
GATTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA 
GATGAAGCCC CCCTGGAGAG AAAAGACCCC TCTGAAGACA AGGAGGACAT CGAAAGCAAC 
CTGCTTAGGC CGATCGGCGT GGCCCTCCGT GGAGCGCACT TCTGTCTGAA GGTCTTCAGG 
GCCGAGGACT TGCCACAGAT GGACGATGCC GTGATGGACA ACGTGAGGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACG GCCAATCCTC AGTGGAACCA GAGCATCACA 
CTGCCTGCCA TGTTTCCATC CATGTGTGAG AAAATGAGGA TTCGTGTCGT AGACTGGGAC 
CGCCTCACCC ACAACGATAT TGTGGCCACC ACCTACCTGA GTATGTCGAA GATCTCTGCC 
TCTGGAGGCG AGATAGAAGT GGACGACCAT CTAGGCTTCC TCCCCACCTT CGGGCCCTGC 
TACATCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGATCC CTATGCAGAG 
CTCAACACGG GCAAGGGAGA AGGCGTGGCC TACCGAGGCC GGCTCCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCGCAG TGAACAGAAA GTGGAGGCTC TCTCTGCCGA TGACATCCTT 
CGGGTAGAGA AGTACCTCCG GAGGCGCAAG TACTCGCTCT TTGCCGCCTT CTACTCGGCC 
ACCATGCTGC AGGATGTCGA TGACGCCGTC CAGTTCGAGG TCAGCATTGG GAACTATGGC 
AACAAGTTCG ACACCACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGATGGAT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCGTCTTACT GGGAGGACAT CCGCCACAGA GTTGAGGCTC AGAACCAGCT TCTCAGGATT 
GCCGACCAGC TGGAAGCCGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCTCC 
GACGAGGACG CGGACTCCCT GGTGGCTCAG CTGATGGATG AGCTCATCGC AGGCTGCAGC 
CAACCCCTGG GTGATGTCCA GGAGATGCCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
CAGCTGCGCA CCCGCCACCT GAGCCAGATC ACCGAGGCGG CCCAGGCCCT GAAGCTCGGC 
CACAGTGAGC TCCCCGCTGC CCTGGAGCAG GCTGAGGACT GGCTCCTGCG GCTCCGTGCC 
CTGGCTGACG AGCCCCAGAA CAGCCTGCCT GACATCGTCA TCTGGATGCT TCAGGGGGAC 
AAGCGGGTGG CGTACCAGCG GGTGCCTGCC CATGAGGTCC TCTTCTCCCG CCGGGGCACC 
AGCTACTGTG GCAAGAACTG TGGAAAACTG CAGACGATAT TTCTGAAATA TCCGATGGAA 
GGGGTGCCCA GAGCCCGGAT GCCGGTGCAG ATTCGGGTCA GGCTCTGGTT TGGACTCTCT 
GTGGATGAAA AGGAGTTCAA CCAGTTTGCT GAGGGCAAGC TCTCCGTCTT CGCTGAAACT 
TATGAGAACC AGACCAAGCT GGCCCTGGTT GGGAACTGGG GCACAACTGG CCTCACCTAC 
CCCAAGTTTT CTGACATCAC GGGCAGGATC AAGCTACCCA AGGACAGCTT CCGCCCCTCA 
GCTGGCTGGG CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACACT GCTTCACGAC 
ACTGATGCTG GCCACCTGAG CTTCGTGGAG GAGGTATTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAACGGGGA GAAGGTGCTT 
CCCAAGGATG ACATCGAGTG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTGAATC GGGCTGTCGA TGAACAAGGC TGGGAGTACA GCATCACCAT CCCGCCGGAC 
CGGAAGCCGA GGCACTGGGT CCCTGCCGAG AAGATGTACT ACACACACCG GCGGCGGCGC 
TGGGTCCGTC TGCGCCGGAG GGACCTCAGC CAGATGGAGG CGTTGAAGCG GCACAGGCAG 
GCGGAGGCAG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TCGGCTGGAA GTTCCATCTG 
GAGTACCGCA AGACAGACGC CTTTCGTCGT CGCCGCTGGC GCCGCCGCAT GGAGCCCTTG 
GAGAAGACAG GGCCTGCGGC TGTGTTTGCC CTTGAGGGGG CCCTGGGTGG CGTGGTGGAC 
GACAGGAGTG AAGATTCCGT GTCCGTCTCC ACCTTGAGTT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC 
CGGGATCTGC CTGCAATGGA CAAGGACTCT TTTTCCGATC CCTATGCAGT CGTCTCCTTC 
CTGCACCAAA GCCAGAAGAC GGTGGTGGCT AAGAACACCC TAAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTACGAGAT CGAGATCTTT GGCGAGCCAA GCAGCATCGC CGAGCAGCCT 
CCCAGCATCG TGGTGGAGCT GTATGACCAT GACACCTATG GTGTAGATGA GTTCATGGGA 
CGTTGCATCT GTCAGCCGAG TCTGGAACGG ACGCCTCGGC TGGCCTGGTT CCCGCTGACG 
AGGGGCAGCC AGCCCGCCGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCTGCCATCC ACCATATTCC TGGTTTTGAG GTACAGGACA CAACAGGGAT CCTGGAAGAG 
TCAGAGGACA CGGACCTGCC CTACCCACCG CCCCAGAGGG AGGCCAACAT CTACATGGTC 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGA ACCGCCATCG AGATCCTGGC GTGGGGTCTT 
CGGAACATGA AGAGCTACCA GCTGGCCAGC GTGTCCTCCC CGAGCCTGGT GGTGGAGTGC 
GGGGGCCAGT CTGTACAATC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTGTACTG TCCCCCCATC 
GTGGTCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CCGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGGGCTT CCTGTGTGAT CCTTACTCAG AGGAGAGTCC GTCCCCACAG 
GGTGGCCCAG ATGACGTGAG CTTACTCAGT CCTGGGGAAG ATGTGCTGAT TGACATCGAT 
GACAAGGAGC CCCTCATCCC CATCCAGGAG GAGGAGTTCA TTGATTGGTG GAGCAAATTC 
TTTGCTTCAA TTGGGGAGAG CGAAAAGTGC GGGTCCTACC TAGAGAAGGA CTTTGACACT 
CTAAAGGTCT ATGACACATC CCTGGAGAAT GTGAAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAATACAT TCAAGCTCTA CCGGGGCAAG ACACAGGAGG AGACTGAAGA CCCATCAGTC 
ATTGGGGAGT TTAAGGGACT CTTCAAAATT TATCCTCTCC CAGAAGACCC AGCCATCCCC 
TTGCCCCCGA GGCAGTTCCA CCAGCTGGCT TCCCAGGGGC CCCAGGAATG CCTGGTCCGC 
GTCTACATTA TCCGGGCGTT TGGCCTGCAG CCCAAGGATC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCG GTGAGTGACC AGGACAGCTA CATCCCTTGT 
ACTCTGGAGC CTGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACCCTCTA TGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGGGAG 
ACGGTCATTG ACCTGGAGAA CAGGCTGCTG TCTAAGTTCG GGGCCCGCTG TGGACTCCCC 
CAGACCTACT GCGTCTCTGG ACCCAACCAG TGGAGGGATC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTGT TCTGCCAGCA GCACCGAGTC AAGGCCCCCG TGTACCGGAC GGACCGCGTG 
GTGTTTCAGG ATAAAGAATA CACCGTCGAA GAAATAGAGG CTGGCAGGGT CCCGAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCC TTGCACGTCC TCCAGCAGCA GGGGCTGATC 
CCTGAGCACG TGGAGTCCCG GCCTCTCTAC AGCCCCCTGC AGCCGGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAACACCAAG 
GATGTGATCC TGGATGACCT CAGCATCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGG TTGGCTTTGA AGAACACAAG CAAAAGACAG ATGTACATTA TCGGTCCCTG 
GGAGGTGAAG GCAACTTTAA CTGGAGGTTC GTCTTCCCTT TTGACTACCT GCCAGCTGAG 
CAGGTCTGCA CCGTCTCCAA GAAGGATGCA TTCTGGAGGC TGGACAAGAC GGAGAGCAAG 
ATCCCTGCAC GAGTGGTATT CCAGATCTGG GACAACGACA AGTTCTCCTT CGATGACTTT 
CTGGGCTCTC TGCAGCTGGA CCTTAACCAC ATGCCCAAGC CCGCCAAGAC AGCTGAGAAG 
TGCTCTGCGG ACCAGCTGGA AGACACCTTC CACCCAGAGC GGTTTGTGTC TCTTTTTGAA 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTGGCGGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAGATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCA 
GCCGGCCACG GCCGGGATGA ACCTAACATG AACCCCAAGC TCGAGGACCC AAGGCGGCCT 
GACACCTCCT TCCTCTGGTT CACCTCCCCG TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGGTTCCGCT GTGCCATCAT CTTCTTCCTC ATTCTCTTCA TCTTCCTGCT CTTCTTGGGC 
ATCTTTGTCT ACTCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212549.1
CDS	539..6838
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212492.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212492.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGGTG GGGGCCAGAG CCTGGCCGAG 
ACCTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAC ACTCTGGAAG GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC GGGAGACGAG 
GCCGAGCCAT TCCTGGACCA GAGCGGGGCT CTGGGCCCGG GGGCCCCCAG CACCCCGAAG 
AAACAACCTT CCCATCCGCC CCCCTACCAC CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT 
GCGCCCAGCA AGCCTCTGTC GGACAAGCCG CAGGACTTCC AGATCAGGGT CCAGGTGATC 
AAGGGACGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG 
CAGACCAAGC GGACGCGGAT CCACAAAGGA AACAGCCCCG TCTTCAATGA GACTCTCTTC 
TTCAACGTGT TTGACTCTCC CGCGGAGCTG TTCAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGAAC AGATGCTCTC ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC 
ATCTACCGAG AGCCCCGACA TGCCTACCTC AGGAAGTGGC TGCTGCTCTC AGATCCCGAC 
GATTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA 
GATGAAGCCC CCCTGGAGAG AAAAGACCCC TCTGAAGACA AGGAGGACAT CGAAAGCAAC 
CTGCTTAGGC CGATCGGCGT GGCCCTCCGT GGAGCGCACT TCTGTCTGAA GGTCTTCAGG 
GCCGAGGACT TGCCACAGAT GGACGATGCC GTGATGGACA ACGTGAGGCA GATCTTCGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACG GCCAATCCTC AGTGGAACCA GAGCATCACA 
CTGCCTGCCA TGTTTCCATC CATGTGTGAG AAAATGAGGA TTCGTGTCGT AGACTGGGAC 
CGCCTCACCC ACAACGATAT TGTGGCCACC ACCTACCTGA GTATGTCGAA GATCTCTGCC 
TCTGGAGGCG AGATAGAAGT GGACGACCAT CTAGGCTTCC TCCCCACCTT CGGGCCCTGC 
TACATCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGATCC CTATGCAGAG 
CTCAACACGG GCAAGGGAGA AGGCGTGGCC TACCGAGGCC GGCTCCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCGCAG TGAACAGAAA GTGGAGGCTC TCTCTGCCGA TGACATCCTT 
CGGGTAGAGA AGTACCTCCG GAGGCGCAAG TACTCGCTCT TTGCCGCCTT CTACTCGGCC 
ACCATGCTGC AGGATGTCGA TGACGCCGTC CAGTTCGAGG TCAGCATTGG GAACTATGGC 
AACAAGTTCG ACACCACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGATGGAT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCGTCTTACT GGGAGGACAT CCGCCACAGA GTTGAGGCTC AGAACCAGCT TCTCAGGATT 
GCCGACCAGC TGGAAGCCGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCTCC 
GACGAGGACG CGGACTCCCT GGTGGCTCAG CTGATGGATG AGCTCATCGC AGGCTGCAGC 
CAACCCCTGG GTGATGTCCA GGAGATGCCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
CAGCTGCGCA CCCGCCACCT GAGCCAGATC ACCGAGGCGG CCCAGGCCCT GAAGCTCGGC 
CACAGTGAGC TCCCCGCTGC CCTGGAGCAG GCTGAGGACT GGCTCCTGCG GCTCCGTGCC 
CTGGCTGACG AGCCCCAGAA CAGCCTGCCT GACATCGTCA TCTGGATGCT TCAGGGGGAC 
AAGCGGGTGG CGTACCAGCG GGTGCCTGCC CATGAGGTCC TCTTCTCCCG CCGGGGCACC 
AGCTACTGTG GCAAGAACTG TGGAAAACTG CAGACGATAT TTCTGAAATA TCCGATGGAA 
GGGGTGCCCA GAGCCCGGAT GCCGGTGCAG ATTCGGGTCA GGCTCTGGTT TGGACTCTCT 
GTGGATGAAA AGGAGTTCAA CCAGTTTGCT GAGGGCAAGC TCTCCGTCTT CGCTGAAACT 
TATGAGAACC AGACCAAGCT GGCCCTGGTT GGGAACTGGG GCACAACTGG CCTCACCTAC 
CCCAAGTTTT CTGACATCAC GGGCAGGATC AAGCTACCCA AGGACAGCTT CCGCCCCTCA 
GCTGGCTGGG CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACACT GCTTCACGAC 
ACTGATGCTG GCCACCTGAG CTTCGTGGAG GAGGTATTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAACGGGGA GAAGGTGCTT 
CCCAAGGATG ACATCGAGTG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTGAATC GGGCTGTCGA TGAACAAGGC TGGGAGTACA GCATCACCAT CCCGCCGGAC 
CGGAAGCCGA GGCACTGGGT CCCTGCCGAG AAGATGTACT ACACACACCG GCGGCGGCGC 
TGGGTCCGTC TGCGCCGGAG GGACCTCAGC CAGATGGAGG CGTTGAAGCG GCACAGGCAG 
GCGGAGGCAG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TCGGCTGGAA GTTCCATCTG 
GAGTACCGCA AGACAGACGC CTTTCGTCGT CGCCGCTGGC GCCGCCGCAT GGAGCCCTTG 
GAGAAGACAG GGCCTGCGGC TGTGTTTGCC CTTGAGGGGG CCCTGGGTGG CGTGGTGGAC 
GACAGGAGTG AAGATTCCGT GTCCGTCTCC ACCTTGAGTT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTAC GCTGCTACAT GTACCAGGCC 
CGGGATCTGC CTGCAATGGA CAAGGACTCT TTTTCCGATC CCTATGCAGT CGTCTCCTTC 
CTGCACCAAA GCCAGAAGAC GGTGGTGGCT AAGAACACCC TAAACCCCAC CTGGGACCAG 
ACGCTCATCT TCTACGAGAT CGAGATCTTT GGCGAGCCAA GCAGCATCGC CGAGCAGCCT 
CCCAGCATCG TGGTGGAGCT GTATGACCAT GACACCTATG GTGTAGATGA GTTCATGGGA 
CGTTGCATCT GTCAGCCGAG TCTGGAACGG ACGCCTCGGC TGGCCTGGTT CCCGCTGACG 
AGGGGCAGCC AGCCCGCCGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCTGCCATCC ACCATATTCC TGGTTTTGAG GTACAGGACA CAACAGGGAT CCTGGAAGAG 
TCAGAGGACA CGGACCTGCC CTACCCACCG CCCCAGAGGG AGGCCAACAT CTACATGGTC 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGA ACCGCCATCG AGATCCTGGC GTGGGGTCTT 
CGGAACATGA AGAGCTACCA GCTGGCCAGC GTGTCCTCCC CGAGCCTGGT GGTGGAGTGC 
GGGGGCCAGT CTGTACAATC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTGTACTG TCCCCCCATC 
GTGGTCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CCGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGGGCTT CCTGTGTGAT CCTTACTCAG AGGAGAGTCC GTCCCCACAG 
GGTGGCCCAG ATGACGTGAG CTTACTCAGT CCTGGGGAAG ATGTGCTGAT TGACATCGAT 
GACAAGGAGC CCCTCATCCC CATCCAGGAG GAGGAGTTCA TTGATTGGTG GAGCAAATTC 
TTTGCTTCAA TTGGGGAGAG CGAAAAGTGC GGGTCCTACC TAGAGAAGGA CTTTGACACT 
CTAAAGGTCT ATGACACATC CCTGGAGAAT GTGAAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAATACAT TCAAGCTCTA CCGGGGCAAG ACACAGGAGG AGACTGAAGA CCCATCAGTC 
ATTGGGGAGT TTAAGGGACT CTTCAAAATT TATCCTCTCC CAGAAGACCC AGCCATCCCC 
TTGCCCCCGA GGCAGTTCCA CCAGCTGGCT TCCCAGGGGC CCCAGGAATG CCTGGTCCGC 
GTCTACATTA TCCGGGCGTT TGGCCTGCAG CCCAAGGATC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCG GTGAGTGACC AGGACAGCTA CATCCCTTGT 
ACTCTGGAGC CTGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACCCTCTA TGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGGGAG 
ACGGTCATTG ACCTGGAGAA CAGGCTGCTG TCTAAGTTCG GGGCCCGCTG TGGACTCCCC 
CAGACCTACT GCGTCTCTGG ACCCAACCAG TGGAGGGATC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTGT TCTGCCAGCA GCACCGAGTC AAGGCCCCCG TGTACCGGAC GGACCGCGTG 
GTGTTTCAGG ATAAAGAATA CACCGTCGAA GAAATAGAGG CTGGCAGGGT CCCGAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCC TTGCACGTCC TCCAGCAGCA GGGGCTGATC 
CCTGAGCACG TGGAGTCCCG GCCTCTCTAC AGCCCCCTGC AGCCGGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAACACCAAG 
GATGTGATCC TGGATGACCT CAGCATCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGG TTGGCTTTGA AGAACACAAG CAAAAGACAG ATGTACATTA TCGGTCCCTG 
GGAGGTGAAG GCAACTTTAA CTGGAGGTTC GTCTTCCCTT TTGACTACCT GCCAGCTGAG 
CAGGTCTGCA CCGTCTCCAA GAAGGATGCA TTCTGGAGGC TGGACAAGAC GGAGAGCAAG 
ATCCCTGCAC GAGTGGTATT CCAGATCTGG GACAACGACA AGTTCTCCTT CGATGACTTT 
CTGGGCTCTC TGCAGCTGGA CCTTAACCAC ATGCCCAAGC CCGCCAAGAC AGCTGAGAAG 
TGCTCTGCGG ACCAGCTGGA AGACACCTTC CACCCAGAGC GGTTTGTGTC TCTTTTTGAA 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTGGCGGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAGATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCA 
GCCGGCCACG GCCGGGATGA ACCTAACATG AACCCCAAGC TCGAGGACCC AAGGCGGCCT 
GACACCTCCT TCCTCTGGTT CACCTCCCCG TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGGTTCCGCT GTGCCATCAT CTTCTTCCTC ATTCTCTTCA TCTTCCTGCT CTTCTTGGGC 
ATCTTTGTCT ACTCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212493.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212550.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa23818
Clone ID Related Accession (Same CDS sequence)	XM_005212493.3
Accession Version	XM_005212493.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212493.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGT GGACGACCAT 
CTAGGCTTCC TCCCCACCTT CGGGCCCTGC TACATCAACC TCTATGGCAG CCCCAGGGAG 
TTCACTGGCT TCCCGGATCC CTATGCAGAG CTCAACACGG GCAAGGGAGA AGGCGTGGCC 
TACCGAGGCC GGCTCCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCGCAG TGAACAGAAA 
GTGGAGGCTC TCTCTGCCGA TGACATCCTT CGGGTAGAGA AGTACCTCCG GAGGCGCAAG 
TACTCGCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTGC AGGATGTCGA TGACGCCGTC 
CAGTTCGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACCACCTG CCTGCCGCTG 
GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGATGGAT GCCACTACTA CTACTTACCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCGTCTTACT GGGAGGACAT CCGCCACAGA 
GTTGAGGCTC AGAACCAGCT TCTCAGGATT GCCGACCAGC TGGAAGCCGG CCTGGAGCAA 
GTCCACTTGG CCCTGAAGGC TCAGTGCTCC GACGAGGACG CGGACTCCCT GGTGGCTCAG 
CTGATGGATG AGCTCATCGC AGGCTGCAGC CAACCCCTGG GTGATGTCCA GGAGATGCCC 
TCTGCCACCC ACCTGGACCA GTACCTGTAC CAGCTGCGCA CCCGCCACCT GAGCCAGATC 
ACCGAGGCGG CCCAGGCCCT GAAGCTCGGC CACAGTGAGC TCCCCGCTGC CCTGGAGCAG 
GCTGAGGACT GGCTCCTGCG GCTCCGTGCC CTGGCTGACG AGCCCCAGAA CAGCCTGCCT 
GACATCGTCA TCTGGATGCT TCAGGGGGAC AAGCGGGTGG CGTACCAGCG GGTGCCTGCC 
CATGAGGTCC TCTTCTCCCG CCGGGGCACC AGCTACTGTG GCAAGAACTG TGGAAAACTG 
CAGACGATAT TTCTGAAATA TCCGATGGAA GGGGTGCCCA GAGCCCGGAT GCCGGTGCAG 
ATTCGGGTCA GGCTCTGGTT TGGACTCTCT GTGGATGAAA AGGAGTTCAA CCAGTTTGCT 
GAGGGCAAGC TCTCCGTCTT CGCTGAAACT TATGAGAACC AGACCAAGCT GGCCCTGGTT 
GGGAACTGGG GCACAACTGG CCTCACCTAC CCCAAGTTTT CTGACATCAC GGGCAGGATC 
AAGCTACCCA AGGACAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCTGG AGACTGGTTC 
GTGTGTCCAG AGAAGACACT GCTTCACGAC ACTGATGCTG GCCACCTGAG CTTCGTGGAG 
GAGGTATTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACGGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAGTG CCCGCTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTGAATC GGGCTGTCGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT CCCGCCGGAC CGGAAGCCGA GGCACTGGGT CCCTGCCGAG 
AAGATGTACT ACACACACCG GCGGCGGCGC TGGGTCCGTC TGCGCCGGAG GGACCTCAGC 
CAGATGGAGG CGTTGAAGCG GCACAGGCAG GCGGAGGCAG AGGGCGAGGG CTGGGAGTAC 
GCCTCGCTCT TCGGCTGGAA GTTCCATCTG GAGTACCGCA AGACAGACGC CTTTCGTCGT 
CGCCGCTGGC GCCGCCGCAT GGAGCCCTTG GAGAAGACAG GGCCTGCGGC TGTGTTTGCC 
CTTGAGGGGG CCCTGGGTGG CGTGGTGGAC GACAGGAGTG AAGATTCCGT GTCCGTCTCC 
ACCTTGAGTT TTGGTGTGAA CAGACCCACG ATTTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTAC GCTGCTACAT GTACCAGGCC CGGGATCTGC CTGCAATGGA CAAGGACTCT 
TTTTCCGATC CCTATGCAGT CGTCTCCTTC CTGCACCAAA GCCAGAAGAC GGTGGTGGCT 
AAGAACACCC TAAACCCCAC CTGGGACCAG ACGCTCATCT TCTACGAGAT CGAGATCTTT 
GGCGAGCCAA GCAGCATCGC CGAGCAGCCT CCCAGCATCG TGGTGGAGCT GTATGACCAT 
GACACCTATG GTGTAGATGA GTTCATGGGA CGTTGCATCT GTCAGCCGAG TCTGGAACGG 
ACGCCTCGGC TGGCCTGGTT CCCGCTGACG AGGGGCAGCC AGCCCGCCGG TGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCTGCCATCC ACCATATTCC TGGTTTTGAG 
GTACAGGACA CAACAGGGAT CCTGGAAGAG TCAGAGGACA CGGACCTGCC CTACCCACCG 
CCCCAGAGGG AGGCCAACAT CTACATGGTC CCCCAGAACA TCAAGCCGGT TCTCCAGCGA 
ACCGCCATCG AGATCCTGGC GTGGGGTCTT CGGAACATGA AGAGCTACCA GCTGGCCAGC 
GTGTCCTCCC CGAGCCTGGT GGTGGAGTGC GGGGGCCAGT CTGTACAATC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCAGGGAGG AGCTGTACTG TCCCCCCATC GTGGTCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CCGTGGTGGG CCAGTGTACC ATCCGCTCCC TGGAGGGCTT CCTGTGTGAT 
CCTTACTCAG AGGAGAGTCC GTCCCCACAG GGTGGCCCAG ATGACGTGAG CTTACTCAGT 
CCTGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATCCAGTTT 
GCAGATGGTC TCTCGGGCTT GGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCTTCAT 
GAGGAGGAGT TCATTGATTG GTGGAGCAAA TTCTTTGCTT CAATTGGGGA GAGCGAAAAG 
TGCGGGTCCT ACCTAGAGAA GGACTTTGAC ACTCTAAAGG TCTATGACAC ATCCCTGGAG 
AATGTGAAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAATA CATTCAAGCT CTACCGGGGC 
AAGACACAGG AGGAGACTGA AGACCCATCA GTCATTGGGG AGTTTAAGGG ACTCTTCAAA 
ATTTATCCTC TCCCAGAAGA CCCAGCCATC CCCTTGCCCC CGAGGCAGTT CCACCAGCTG 
GCTTCCCAGG GGCCCCAGGA ATGCCTGGTC CGCGTCTACA TTATCCGGGC GTTTGGCCTG 
CAGCCCAAGG ATCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCGGTGAGTG ACCAGGACAG CTACATCCCT TGTACTCTGG AGCCTGTATT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGGTCACCCT CTATGACTAT 
GACCTCCTCT CCAAGGATGA AAAGATTGGG GAGACGGTCA TTGACCTGGA GAACAGGCTG 
CTGTCTAAGT TCGGGGCCCG CTGTGGACTC CCCCAGACCT ACTGCGTCTC TGGACCCAAC 
CAGTGGAGGG ATCAGCTCCG CCCCTCCCAG CTCCTCCACC TGTTCTGCCA GCAGCACCGA 
GTCAAGGCCC CCGTGTACCG GACGGACCGC GTGGTGTTTC AGGATAAAGA ATACACCGTC 
GAAGAAATAG AGGCTGGCAG GGTCCCGAAC CCACACCTGG GCCCAGTGGA GGAGCGTCTG 
GCCTTGCACG TCCTCCAGCA GCAGGGGCTG ATCCCTGAGC ACGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCGGA CATCGAGCAG GGGAAGCTGC AGATGTGGGT CGACCTGTTT 
CCGAAGGCCC TGGGGCGGCC GGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAACACC AAGGATGTGA TCCTGGATGA CCTCAGCATC 
ACGGGGGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGGTTGGCTT TGAAGAACAC 
AAGCAAAAGA CAGATGTACA TTATCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCGTCTTCC CTTTTGACTA CCTGCCAGCT GAGCAGGTCT GCACCGTCTC CAAGAAGGAT 
GCATTCTGGA GGCTGGACAA GACGGAGAGC AAGATCCCTG CACGAGTGGT ATTCCAGATC 
TGGGACAACG ACAAGTTCTC CTTCGATGAC TTTCTGGGCT CTCTGCAGCT GGACCTTAAC 
CACATGCCCA AGCCCGCCAA GACAGCTGAG AAGTGCTCTG CGGACCAGCT GGAAGACACC 
TTCCACCCAG AGCGGTTTGT GTCTCTTTTT GAACAGAAAA CAGTGAAGGG CTGGTGGCCC 
TGTGTGGCGG AAGAGGGTGA GAAGAAGATA CTGGCGGGCA AGCTGGAGAT GACCTTGGAG 
ATTGTAACAG AGAGTGAGCA TGAGGAGCGG CCAGCCGGCC ACGGCCGGGA TGAACCTAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGG CCTGACACCT CCTTCCTCTG GTTCACCTCC 
CCGTACAAGA CCATGAAGTT CATCCTGTGG CGGCGGTTCC GCTGTGCCAT CATCTTCTTC 
CTCATTCTCT TCATCTTCCT GCTCTTCTTG GGCATCTTTG TCTACTCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212550.1
CDS	130..6402
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212493.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212493.3

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGT GGACGACCAT 
CTAGGCTTCC TCCCCACCTT CGGGCCCTGC TACATCAACC TCTATGGCAG CCCCAGGGAG 
TTCACTGGCT TCCCGGATCC CTATGCAGAG CTCAACACGG GCAAGGGAGA AGGCGTGGCC 
TACCGAGGCC GGCTCCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCGCAG TGAACAGAAA 
GTGGAGGCTC TCTCTGCCGA TGACATCCTT CGGGTAGAGA AGTACCTCCG GAGGCGCAAG 
TACTCGCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTGC AGGATGTCGA TGACGCCGTC 
CAGTTCGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACCACCTG CCTGCCGCTG 
GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGATGGAT GCCACTACTA CTACTTACCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCGTCTTACT GGGAGGACAT CCGCCACAGA 
GTTGAGGCTC AGAACCAGCT TCTCAGGATT GCCGACCAGC TGGAAGCCGG CCTGGAGCAA 
GTCCACTTGG CCCTGAAGGC TCAGTGCTCC GACGAGGACG CGGACTCCCT GGTGGCTCAG 
CTGATGGATG AGCTCATCGC AGGCTGCAGC CAACCCCTGG GTGATGTCCA GGAGATGCCC 
TCTGCCACCC ACCTGGACCA GTACCTGTAC CAGCTGCGCA CCCGCCACCT GAGCCAGATC 
ACCGAGGCGG CCCAGGCCCT GAAGCTCGGC CACAGTGAGC TCCCCGCTGC CCTGGAGCAG 
GCTGAGGACT GGCTCCTGCG GCTCCGTGCC CTGGCTGACG AGCCCCAGAA CAGCCTGCCT 
GACATCGTCA TCTGGATGCT TCAGGGGGAC AAGCGGGTGG CGTACCAGCG GGTGCCTGCC 
CATGAGGTCC TCTTCTCCCG CCGGGGCACC AGCTACTGTG GCAAGAACTG TGGAAAACTG 
CAGACGATAT TTCTGAAATA TCCGATGGAA GGGGTGCCCA GAGCCCGGAT GCCGGTGCAG 
ATTCGGGTCA GGCTCTGGTT TGGACTCTCT GTGGATGAAA AGGAGTTCAA CCAGTTTGCT 
GAGGGCAAGC TCTCCGTCTT CGCTGAAACT TATGAGAACC AGACCAAGCT GGCCCTGGTT 
GGGAACTGGG GCACAACTGG CCTCACCTAC CCCAAGTTTT CTGACATCAC GGGCAGGATC 
AAGCTACCCA AGGACAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCTGG AGACTGGTTC 
GTGTGTCCAG AGAAGACACT GCTTCACGAC ACTGATGCTG GCCACCTGAG CTTCGTGGAG 
GAGGTATTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACGGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAGTG CCCGCTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTGAATC GGGCTGTCGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT CCCGCCGGAC CGGAAGCCGA GGCACTGGGT CCCTGCCGAG 
AAGATGTACT ACACACACCG GCGGCGGCGC TGGGTCCGTC TGCGCCGGAG GGACCTCAGC 
CAGATGGAGG CGTTGAAGCG GCACAGGCAG GCGGAGGCAG AGGGCGAGGG CTGGGAGTAC 
GCCTCGCTCT TCGGCTGGAA GTTCCATCTG GAGTACCGCA AGACAGACGC CTTTCGTCGT 
CGCCGCTGGC GCCGCCGCAT GGAGCCCTTG GAGAAGACAG GGCCTGCGGC TGTGTTTGCC 
CTTGAGGGGG CCCTGGGTGG CGTGGTGGAC GACAGGAGTG AAGATTCCGT GTCCGTCTCC 
ACCTTGAGTT TTGGTGTGAA CAGACCCACG ATTTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTAC GCTGCTACAT GTACCAGGCC CGGGATCTGC CTGCAATGGA CAAGGACTCT 
TTTTCCGATC CCTATGCAGT CGTCTCCTTC CTGCACCAAA GCCAGAAGAC GGTGGTGGCT 
AAGAACACCC TAAACCCCAC CTGGGACCAG ACGCTCATCT TCTACGAGAT CGAGATCTTT 
GGCGAGCCAA GCAGCATCGC CGAGCAGCCT CCCAGCATCG TGGTGGAGCT GTATGACCAT 
GACACCTATG GTGTAGATGA GTTCATGGGA CGTTGCATCT GTCAGCCGAG TCTGGAACGG 
ACGCCTCGGC TGGCCTGGTT CCCGCTGACG AGGGGCAGCC AGCCCGCCGG TGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCTGCCATCC ACCATATTCC TGGTTTTGAG 
GTACAGGACA CAACAGGGAT CCTGGAAGAG TCAGAGGACA CGGACCTGCC CTACCCACCG 
CCCCAGAGGG AGGCCAACAT CTACATGGTC CCCCAGAACA TCAAGCCGGT TCTCCAGCGA 
ACCGCCATCG AGATCCTGGC GTGGGGTCTT CGGAACATGA AGAGCTACCA GCTGGCCAGC 
GTGTCCTCCC CGAGCCTGGT GGTGGAGTGC GGGGGCCAGT CTGTACAATC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCAGGGAGG AGCTGTACTG TCCCCCCATC GTGGTCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CCGTGGTGGG CCAGTGTACC ATCCGCTCCC TGGAGGGCTT CCTGTGTGAT 
CCTTACTCAG AGGAGAGTCC GTCCCCACAG GGTGGCCCAG ATGACGTGAG CTTACTCAGT 
CCTGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATCCAGTTT 
GCAGATGGTC TCTCGGGCTT GGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCTTCAT 
GAGGAGGAGT TCATTGATTG GTGGAGCAAA TTCTTTGCTT CAATTGGGGA GAGCGAAAAG 
TGCGGGTCCT ACCTAGAGAA GGACTTTGAC ACTCTAAAGG TCTATGACAC ATCCCTGGAG 
AATGTGAAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAATA CATTCAAGCT CTACCGGGGC 
AAGACACAGG AGGAGACTGA AGACCCATCA GTCATTGGGG AGTTTAAGGG ACTCTTCAAA 
ATTTATCCTC TCCCAGAAGA CCCAGCCATC CCCTTGCCCC CGAGGCAGTT CCACCAGCTG 
GCTTCCCAGG GGCCCCAGGA ATGCCTGGTC CGCGTCTACA TTATCCGGGC GTTTGGCCTG 
CAGCCCAAGG ATCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCGGTGAGTG ACCAGGACAG CTACATCCCT TGTACTCTGG AGCCTGTATT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGGTCACCCT CTATGACTAT 
GACCTCCTCT CCAAGGATGA AAAGATTGGG GAGACGGTCA TTGACCTGGA GAACAGGCTG 
CTGTCTAAGT TCGGGGCCCG CTGTGGACTC CCCCAGACCT ACTGCGTCTC TGGACCCAAC 
CAGTGGAGGG ATCAGCTCCG CCCCTCCCAG CTCCTCCACC TGTTCTGCCA GCAGCACCGA 
GTCAAGGCCC CCGTGTACCG GACGGACCGC GTGGTGTTTC AGGATAAAGA ATACACCGTC 
GAAGAAATAG AGGCTGGCAG GGTCCCGAAC CCACACCTGG GCCCAGTGGA GGAGCGTCTG 
GCCTTGCACG TCCTCCAGCA GCAGGGGCTG ATCCCTGAGC ACGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCGGA CATCGAGCAG GGGAAGCTGC AGATGTGGGT CGACCTGTTT 
CCGAAGGCCC TGGGGCGGCC GGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAACACC AAGGATGTGA TCCTGGATGA CCTCAGCATC 
ACGGGGGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGGTTGGCTT TGAAGAACAC 
AAGCAAAAGA CAGATGTACA TTATCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCGTCTTCC CTTTTGACTA CCTGCCAGCT GAGCAGGTCT GCACCGTCTC CAAGAAGGAT 
GCATTCTGGA GGCTGGACAA GACGGAGAGC AAGATCCCTG CACGAGTGGT ATTCCAGATC 
TGGGACAACG ACAAGTTCTC CTTCGATGAC TTTCTGGGCT CTCTGCAGCT GGACCTTAAC 
CACATGCCCA AGCCCGCCAA GACAGCTGAG AAGTGCTCTG CGGACCAGCT GGAAGACACC 
TTCCACCCAG AGCGGTTTGT GTCTCTTTTT GAACAGAAAA CAGTGAAGGG CTGGTGGCCC 
TGTGTGGCGG AAGAGGGTGA GAAGAAGATA CTGGCGGGCA AGCTGGAGAT GACCTTGGAG 
ATTGTAACAG AGAGTGAGCA TGAGGAGCGG CCAGCCGGCC ACGGCCGGGA TGAACCTAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGG CCTGACACCT CCTTCCTCTG GTTCACCTCC 
CCGTACAAGA CCATGAAGTT CATCCTGTGG CGGCGGTTCC GCTGTGCCAT CATCTTCTTC 
CTCATTCTCT TCATCTTCCT GCTCTTCTTG GGCATCTTTG TCTACTCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212494.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212551.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBa23819
Clone ID Related Accession (Same CDS sequence)	XM_005212494.3
Accession Version	XM_005212494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGTGGA CGACCATCTA 
GGCTTCCTCC CCACCTTCGG GCCCTGCTAC ATCAACCTCT ATGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGATCCCTA TGCAGAGCTC AACACGGGCA AGGGAGAAGG CGTGGCCTAC 
CGAGGCCGGC TCCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCGCAGTGA ACAGAAAGTG 
GAGGCTCTCT CTGCCGATGA CATCCTTCGG GTAGAGAAGT ACCTCCGGAG GCGCAAGTAC 
TCGCTCTTTG CCGCCTTCTA CTCGGCCACC ATGCTGCAGG ATGTCGATGA CGCCGTCCAG 
TTCGAGGTCA GCATTGGGAA CTATGGCAAC AAGTTCGACA CCACCTGCCT GCCGCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTACTA CTTACCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCG TCTTACTGGG AGGACATCCG CCACAGAGTT 
GAGGCTCAGA ACCAGCTTCT CAGGATTGCC GACCAGCTGG AAGCCGGCCT GGAGCAAGTC 
CACTTGGCCC TGAAGGCTCA GTGCTCCGAC GAGGACGCGG ACTCCCTGGT GGCTCAGCTG 
ATGGATGAGC TCATCGCAGG CTGCAGCCAA CCCCTGGGTG ATGTCCAGGA GATGCCCTCT 
GCCACCCACC TGGACCAGTA CCTGTACCAG CTGCGCACCC GCCACCTGAG CCAGATCACC 
GAGGCGGCCC AGGCCCTGAA GCTCGGCCAC AGTGAGCTCC CCGCTGCCCT GGAGCAGGCT 
GAGGACTGGC TCCTGCGGCT CCGTGCCCTG GCTGACGAGC CCCAGAACAG CCTGCCTGAC 
ATCGTCATCT GGATGCTTCA GGGGGACAAG CGGGTGGCGT ACCAGCGGGT GCCTGCCCAT 
GAGGTCCTCT TCTCCCGCCG GGGCACCAGC TACTGTGGCA AGAACTGTGG AAAACTGCAG 
ACGATATTTC TGAAATATCC GATGGAAGGG GTGCCCAGAG CCCGGATGCC GGTGCAGATT 
CGGGTCAGGC TCTGGTTTGG ACTCTCTGTG GATGAAAAGG AGTTCAACCA GTTTGCTGAG 
GGCAAGCTCT CCGTCTTCGC TGAAACTTAT GAGAACCAGA CCAAGCTGGC CCTGGTTGGG 
AACTGGGGCA CAACTGGCCT CACCTACCCC AAGTTTTCTG ACATCACGGG CAGGATCAAG 
CTACCCAAGG ACAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCTGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACACTGCT TCACGACACT GATGCTGGCC ACCTGAGCTT CGTGGAGGAG 
GTATTTGAGA ACCAGACCCG GCTCCCTGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAGTGCCC GCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTGAATCGGG CTGTCGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATCCC GCCGGACCGG AAGCCGAGGC ACTGGGTCCC TGCCGAGAAG 
ATGTACTACA CACACCGGCG GCGGCGCTGG GTCCGTCTGC GCCGGAGGGA CCTCAGCCAG 
ATGGAGGCGT TGAAGCGGCA CAGGCAGGCG GAGGCAGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTCG GCTGGAAGTT CCATCTGGAG TACCGCAAGA CAGACGCCTT TCGTCGTCGC 
CGCTGGCGCC GCCGCATGGA GCCCTTGGAG AAGACAGGGC CTGCGGCTGT GTTTGCCCTT 
GAGGGGGCCC TGGGTGGCGT GGTGGACGAC AGGAGTGAAG ATTCCGTGTC CGTCTCCACC 
TTGAGTTTTG GTGTGAACAG ACCCACGATT TCCTGCATCT TTGACTATGG GAACCGCTAC 
CATCTACGCT GCTACATGTA CCAGGCCCGG GATCTGCCTG CAATGGACAA GGACTCTTTT 
TCCGATCCCT ATGCAGTCGT CTCCTTCCTG CACCAAAGCC AGAAGACGGT GGTGGCTAAG 
AACACCCTAA ACCCCACCTG GGACCAGACG CTCATCTTCT ACGAGATCGA GATCTTTGGC 
GAGCCAAGCA GCATCGCCGA GCAGCCTCCC AGCATCGTGG TGGAGCTGTA TGACCATGAC 
ACCTATGGTG TAGATGAGTT CATGGGACGT TGCATCTGTC AGCCGAGTCT GGAACGGACG 
CCTCGGCTGG CCTGGTTCCC GCTGACGAGG GGCAGCCAGC CCGCCGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCT GCCATCCACC ATATTCCTGG TTTTGAGGTA 
CAGGACACAA CAGGGATCCT GGAAGAGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGTTTGCA 
GATGGTCTCT CGGGCTTGGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCTTCATGAG 
GAGGAGTTCA TTGATTGGTG GAGCAAATTC TTTGCTTCAA TTGGGGAGAG CGAAAAGTGC 
GGGTCCTACC TAGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACATC CCTGGAGAAT 
GTGAAGGCCT TTGAGGGCCT GTCTGACTTT TGTAATACAT TCAAGCTCTA CCGGGGCAAG 
ACACAGGAGG AGACTGAAGA CCCATCAGTC ATTGGGGAGT TTAAGGGACT CTTCAAAATT 
TATCCTCTCC CAGAAGACCC AGCCATCCCC TTGCCCCCGA GGCAGTTCCA CCAGCTGGCT 
TCCCAGGGGC CCCAGGAATG CCTGGTCCGC GTCTACATTA TCCGGGCGTT TGGCCTGCAG 
CCCAAGGATC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGTGACC AGGACAGCTA CATCCCTTGT ACTCTGGAGC CTGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGG TCACCCTCTA TGACTATGAC 
CTCCTCTCCA AGGATGAAAA GATTGGGGAG ACGGTCATTG ACCTGGAGAA CAGGCTGCTG 
TCTAAGTTCG GGGCCCGCTG TGGACTCCCC CAGACCTACT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTGT TCTGCCAGCA GCACCGAGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCGTCGAA 
GAAATAGAGG CTGGCAGGGT CCCGAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCA GGGGCTGATC CCTGAGCACG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATCC TGGATGACCT CAGCATCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ATGTACATTA TCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
GTCTTCCCTT TTGACTACCT GCCAGCTGAG CAGGTCTGCA CCGTCTCCAA GAAGGATGCA 
TTCTGGAGGC TGGACAAGAC GGAGAGCAAG ATCCCTGCAC GAGTGGTATT CCAGATCTGG 
GACAACGACA AGTTCTCCTT CGATGACTTT CTGGGCTCTC TGCAGCTGGA CCTTAACCAC 
ATGCCCAAGC CCGCCAAGAC AGCTGAGAAG TGCTCTGCGG ACCAGCTGGA AGACACCTTC 
CACCCAGAGC GGTTTGTGTC TCTTTTTGAA CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTGGCGGAAG AGGGTGAGAA GAAGATACTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT 
GTAACAGAGA GTGAGCATGA GGAGCGGCCA GCCGGCCACG GCCGGGATGA ACCTAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGGCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGCT GTGCCATCAT CTTCTTCCTC 
ATTCTCTTCA TCTTCCTGCT CTTCTTGGGC ATCTTTGTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212551.1
CDS	539..6808
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212494.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212494.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGTGGA CGACCATCTA 
GGCTTCCTCC CCACCTTCGG GCCCTGCTAC ATCAACCTCT ATGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGATCCCTA TGCAGAGCTC AACACGGGCA AGGGAGAAGG CGTGGCCTAC 
CGAGGCCGGC TCCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCGCAGTGA ACAGAAAGTG 
GAGGCTCTCT CTGCCGATGA CATCCTTCGG GTAGAGAAGT ACCTCCGGAG GCGCAAGTAC 
TCGCTCTTTG CCGCCTTCTA CTCGGCCACC ATGCTGCAGG ATGTCGATGA CGCCGTCCAG 
TTCGAGGTCA GCATTGGGAA CTATGGCAAC AAGTTCGACA CCACCTGCCT GCCGCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTACTA CTTACCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCG TCTTACTGGG AGGACATCCG CCACAGAGTT 
GAGGCTCAGA ACCAGCTTCT CAGGATTGCC GACCAGCTGG AAGCCGGCCT GGAGCAAGTC 
CACTTGGCCC TGAAGGCTCA GTGCTCCGAC GAGGACGCGG ACTCCCTGGT GGCTCAGCTG 
ATGGATGAGC TCATCGCAGG CTGCAGCCAA CCCCTGGGTG ATGTCCAGGA GATGCCCTCT 
GCCACCCACC TGGACCAGTA CCTGTACCAG CTGCGCACCC GCCACCTGAG CCAGATCACC 
GAGGCGGCCC AGGCCCTGAA GCTCGGCCAC AGTGAGCTCC CCGCTGCCCT GGAGCAGGCT 
GAGGACTGGC TCCTGCGGCT CCGTGCCCTG GCTGACGAGC CCCAGAACAG CCTGCCTGAC 
ATCGTCATCT GGATGCTTCA GGGGGACAAG CGGGTGGCGT ACCAGCGGGT GCCTGCCCAT 
GAGGTCCTCT TCTCCCGCCG GGGCACCAGC TACTGTGGCA AGAACTGTGG AAAACTGCAG 
ACGATATTTC TGAAATATCC GATGGAAGGG GTGCCCAGAG CCCGGATGCC GGTGCAGATT 
CGGGTCAGGC TCTGGTTTGG ACTCTCTGTG GATGAAAAGG AGTTCAACCA GTTTGCTGAG 
GGCAAGCTCT CCGTCTTCGC TGAAACTTAT GAGAACCAGA CCAAGCTGGC CCTGGTTGGG 
AACTGGGGCA CAACTGGCCT CACCTACCCC AAGTTTTCTG ACATCACGGG CAGGATCAAG 
CTACCCAAGG ACAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCTGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACACTGCT TCACGACACT GATGCTGGCC ACCTGAGCTT CGTGGAGGAG 
GTATTTGAGA ACCAGACCCG GCTCCCTGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAGTGCCC GCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTGAATCGGG CTGTCGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATCCC GCCGGACCGG AAGCCGAGGC ACTGGGTCCC TGCCGAGAAG 
ATGTACTACA CACACCGGCG GCGGCGCTGG GTCCGTCTGC GCCGGAGGGA CCTCAGCCAG 
ATGGAGGCGT TGAAGCGGCA CAGGCAGGCG GAGGCAGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTCG GCTGGAAGTT CCATCTGGAG TACCGCAAGA CAGACGCCTT TCGTCGTCGC 
CGCTGGCGCC GCCGCATGGA GCCCTTGGAG AAGACAGGGC CTGCGGCTGT GTTTGCCCTT 
GAGGGGGCCC TGGGTGGCGT GGTGGACGAC AGGAGTGAAG ATTCCGTGTC CGTCTCCACC 
TTGAGTTTTG GTGTGAACAG ACCCACGATT TCCTGCATCT TTGACTATGG GAACCGCTAC 
CATCTACGCT GCTACATGTA CCAGGCCCGG GATCTGCCTG CAATGGACAA GGACTCTTTT 
TCCGATCCCT ATGCAGTCGT CTCCTTCCTG CACCAAAGCC AGAAGACGGT GGTGGCTAAG 
AACACCCTAA ACCCCACCTG GGACCAGACG CTCATCTTCT ACGAGATCGA GATCTTTGGC 
GAGCCAAGCA GCATCGCCGA GCAGCCTCCC AGCATCGTGG TGGAGCTGTA TGACCATGAC 
ACCTATGGTG TAGATGAGTT CATGGGACGT TGCATCTGTC AGCCGAGTCT GGAACGGACG 
CCTCGGCTGG CCTGGTTCCC GCTGACGAGG GGCAGCCAGC CCGCCGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCT GCCATCCACC ATATTCCTGG TTTTGAGGTA 
CAGGACACAA CAGGGATCCT GGAAGAGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGTTTGCA 
GATGGTCTCT CGGGCTTGGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCTTCATGAG 
GAGGAGTTCA TTGATTGGTG GAGCAAATTC TTTGCTTCAA TTGGGGAGAG CGAAAAGTGC 
GGGTCCTACC TAGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACATC CCTGGAGAAT 
GTGAAGGCCT TTGAGGGCCT GTCTGACTTT TGTAATACAT TCAAGCTCTA CCGGGGCAAG 
ACACAGGAGG AGACTGAAGA CCCATCAGTC ATTGGGGAGT TTAAGGGACT CTTCAAAATT 
TATCCTCTCC CAGAAGACCC AGCCATCCCC TTGCCCCCGA GGCAGTTCCA CCAGCTGGCT 
TCCCAGGGGC CCCAGGAATG CCTGGTCCGC GTCTACATTA TCCGGGCGTT TGGCCTGCAG 
CCCAAGGATC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGTGACC AGGACAGCTA CATCCCTTGT ACTCTGGAGC CTGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGG TCACCCTCTA TGACTATGAC 
CTCCTCTCCA AGGATGAAAA GATTGGGGAG ACGGTCATTG ACCTGGAGAA CAGGCTGCTG 
TCTAAGTTCG GGGCCCGCTG TGGACTCCCC CAGACCTACT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTGT TCTGCCAGCA GCACCGAGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCGTCGAA 
GAAATAGAGG CTGGCAGGGT CCCGAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCA GGGGCTGATC CCTGAGCACG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATCC TGGATGACCT CAGCATCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ATGTACATTA TCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
GTCTTCCCTT TTGACTACCT GCCAGCTGAG CAGGTCTGCA CCGTCTCCAA GAAGGATGCA 
TTCTGGAGGC TGGACAAGAC GGAGAGCAAG ATCCCTGCAC GAGTGGTATT CCAGATCTGG 
GACAACGACA AGTTCTCCTT CGATGACTTT CTGGGCTCTC TGCAGCTGGA CCTTAACCAC 
ATGCCCAAGC CCGCCAAGAC AGCTGAGAAG TGCTCTGCGG ACCAGCTGGA AGACACCTTC 
CACCCAGAGC GGTTTGTGTC TCTTTTTGAA CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTGGCGGAAG AGGGTGAGAA GAAGATACTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT 
GTAACAGAGA GTGAGCATGA GGAGCGGCCA GCCGGCCACG GCCGGGATGA ACCTAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGGCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGCT GTGCCATCAT CTTCTTCCTC 
ATTCTCTTCA TCTTCCTGCT CTTCTTGGGC ATCTTTGTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212495.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212552.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OBa23820
Clone ID Related Accession (Same CDS sequence)	XM_005212495.3
Accession Version	XM_005212495.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTCCTCC AGCCACAGAA CTGGACGACC ATCTAGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACATCAA CCTCTATGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAT 
CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTCCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCGC AGTGAACAGA AAGTGGAGGC TCTCTCTGCC 
GATGACATCC TTCGGGTAGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT GCAGGATGTC GATGACGCCG TCCAGTTCGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACCACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGATGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC ATCCGCCACA GAGTTGAGGC TCAGAACCAG 
CTTCTCAGGA TTGCCGACCA GCTGGAAGCC GGCCTGGAGC AAGTCCACTT GGCCCTGAAG 
GCTCAGTGCT CCGACGAGGA CGCGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATC 
GCAGGCTGCA GCCAACCCCT GGGTGATGTC CAGGAGATGC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGT ACCAGCTGCG CACCCGCCAC CTGAGCCAGA TCACCGAGGC GGCCCAGGCC 
CTGAAGCTCG GCCACAGTGA GCTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTTCAGGGGG ACAAGCGGGT GGCGTACCAG CGGGTGCCTG CCCATGAGGT CCTCTTCTCC 
CGCCGGGGCA CCAGCTACTG TGGCAAGAAC TGTGGAAAAC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AAGGGGTGCC CAGAGCCCGG ATGCCGGTGC AGATTCGGGT CAGGCTCTGG 
TTTGGACTCT CTGTGGATGA AAAGGAGTTC AACCAGTTTG CTGAGGGCAA GCTCTCCGTC 
TTCGCTGAAA CTTATGAGAA CCAGACCAAG CTGGCCCTGG TTGGGAACTG GGGCACAACT 
GGCCTCACCT ACCCCAAGTT TTCTGACATC ACGGGCAGGA TCAAGCTACC CAAGGACAGC 
TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT GGAGACTGGT TCGTGTGTCC AGAGAAGACA 
CTGCTTCACG ACACTGATGC TGGCCACCTG AGCTTCGTGG AGGAGGTATT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTGAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCGCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC 
CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG AGGGACCTCA GCCAGATGGA GGCGTTGAAG 
CGGCACAGGC AGGCGGAGGC AGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG 
AAGTTCCATC TGGAGTACCG CAAGACAGAC GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC 
ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT 
GGCGTGGTGG ACGACAGGAG TGAAGATTCC GTGTCCGTCT CCACCTTGAG TTTTGGTGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT ACGCTGCTAC 
ATGTACCAGG CCCGGGATCT GCCTGCAATG GACAAGGACT CTTTTTCCGA TCCCTATGCA 
GTCGTCTCCT TCCTGCACCA AAGCCAGAAG ACGGTGGTGG CTAAGAACAC CCTAAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTACGAG ATCGAGATCT TTGGCGAGCC AAGCAGCATC 
GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA TGGTGTAGAT 
GAGTTCATGG GACGTTGCAT CTGTCAGCCG AGTCTGGAAC GGACGCCTCG GCTGGCCTGG 
TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCTGCCAT CCACCATATT CCTGGTTTTG AGGTACAGGA CACAACAGGG 
ATCCTGGAAG AGTCAGAGGA CACGGACCTG CCCTACCCAC CGCCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACCGCCAT CGAGATCCTG 
GCGTGGGGTC TTCGGAACAT GAAGAGCTAC CAGCTGGCCA GCGTGTCCTC CCCGAGCCTG 
GTGGTGGAGT GCGGGGGCCA GTCTGTACAA TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTGTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCCGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGGGC TTCCTGTGTG ATCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGTGGCCC AGATGACGTG AGCTTACTCA GTCCTGGGGA AGATGTGCTG 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATCCAGG AGGAGGAGTT CATTGATTGG 
TGGAGCAAAT TCTTTGCTTC AATTGGGGAG AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG 
GACTTTGACA CTCTAAAGGT CTATGACACA TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAATAC ATTCAAGCTC TACCGGGGCA AGACACAGGA GGAGACTGAA 
GACCCATCAG TCATTGGGGA GTTTAAGGGA CTCTTCAAAA TTTATCCTCT CCCAGAAGAC 
CCAGCCATCC CCTTGCCCCC GAGGCAGTTC CACCAGCTGG CTTCCCAGGG GCCCCAGGAA 
TGCCTGGTCC GCGTCTACAT TATCCGGGCG TTTGGCCTGC AGCCCAAGGA TCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CGGTGAGTGA CCAGGACAGC 
TACATCCCTT GTACTCTGGA GCCTGTATTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GGTCACCCTC TATGACTATG ACCTCCTCTC CAAGGATGAA 
AAGATTGGGG AGACGGTCAT TGACCTGGAG AACAGGCTGC TGTCTAAGTT CGGGGCCCGC 
TGTGGACTCC CCCAGACCTA CTGCGTCTCT GGACCCAACC AGTGGAGGGA TCAGCTCCGC 
CCCTCCCAGC TCCTCCACCT GTTCTGCCAG CAGCACCGAG TCAAGGCCCC CGTGTACCGG 
ACGGACCGCG TGGTGTTTCA GGATAAAGAA TACACCGTCG AAGAAATAGA GGCTGGCAGG 
GTCCCGAACC CACACCTGGG CCCAGTGGAG GAGCGTCTGG CCTTGCACGT CCTCCAGCAG 
CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC 
ATCGAGCAGG GGAAGCTGCA GATGTGGGTC GACCTGTTTC CGAAGGCCCT GGGGCGGCCG 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACCA AGGATGTGAT CCTGGATGAC CTCAGCATCA CGGGGGAGAA GATGAGCGAC 
ATTTATGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTACAT 
TATCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCGTCTTCCC TTTTGACTAC 
CTGCCAGCTG AGCAGGTCTG CACCGTCTCC AAGAAGGATG CATTCTGGAG GCTGGACAAG 
ACGGAGAGCA AGATCCCTGC ACGAGTGGTA TTCCAGATCT GGGACAACGA CAAGTTCTCC 
TTCGATGACT TTCTGGGCTC TCTGCAGCTG GACCTTAACC ACATGCCCAA GCCCGCCAAG 
ACAGCTGAGA AGTGCTCTGC GGACCAGCTG GAAGACACCT TCCACCCAGA GCGGTTTGTG 
TCTCTTTTTG AACAGAAAAC AGTGAAGGGC TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG 
AAGAAGATAC TGGCGGGCAA GCTGGAGATG ACCTTGGAGA TTGTAACAGA GAGTGAGCAT 
GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT GAACCTAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGGC CTGACACCTC CTTCCTCTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG CTGTGCCATC ATCTTCTTCC TCATTCTCTT CATCTTCCTG 
CTCTTCTTGG GCATCTTTGT CTACTCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212552.1
CDS	129..6380
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212495.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212495.3

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTCCTCC AGCCACAGAA CTGGACGACC ATCTAGGCTT CCTCCCCACC 
TTCGGGCCCT GCTACATCAA CCTCTATGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAT 
CCCTATGCAG AGCTCAACAC GGGCAAGGGA GAAGGCGTGG CCTACCGAGG CCGGCTCCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCGC AGTGAACAGA AAGTGGAGGC TCTCTCTGCC 
GATGACATCC TTCGGGTAGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT GCAGGATGTC GATGACGCCG TCCAGTTCGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACCACC TGCCTGCCGC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGATGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCGTCTTA CTGGGAGGAC ATCCGCCACA GAGTTGAGGC TCAGAACCAG 
CTTCTCAGGA TTGCCGACCA GCTGGAAGCC GGCCTGGAGC AAGTCCACTT GGCCCTGAAG 
GCTCAGTGCT CCGACGAGGA CGCGGACTCC CTGGTGGCTC AGCTGATGGA TGAGCTCATC 
GCAGGCTGCA GCCAACCCCT GGGTGATGTC CAGGAGATGC CCTCTGCCAC CCACCTGGAC 
CAGTACCTGT ACCAGCTGCG CACCCGCCAC CTGAGCCAGA TCACCGAGGC GGCCCAGGCC 
CTGAAGCTCG GCCACAGTGA GCTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGGCTCCGTG CCCTGGCTGA CGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTTCAGGGGG ACAAGCGGGT GGCGTACCAG CGGGTGCCTG CCCATGAGGT CCTCTTCTCC 
CGCCGGGGCA CCAGCTACTG TGGCAAGAAC TGTGGAAAAC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AAGGGGTGCC CAGAGCCCGG ATGCCGGTGC AGATTCGGGT CAGGCTCTGG 
TTTGGACTCT CTGTGGATGA AAAGGAGTTC AACCAGTTTG CTGAGGGCAA GCTCTCCGTC 
TTCGCTGAAA CTTATGAGAA CCAGACCAAG CTGGCCCTGG TTGGGAACTG GGGCACAACT 
GGCCTCACCT ACCCCAAGTT TTCTGACATC ACGGGCAGGA TCAAGCTACC CAAGGACAGC 
TTCCGCCCCT CAGCTGGCTG GGCCTGGGCT GGAGACTGGT TCGTGTGTCC AGAGAAGACA 
CTGCTTCACG ACACTGATGC TGGCCACCTG AGCTTCGTGG AGGAGGTATT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAG TGCCCGCTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTGAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCGCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCCG AGAAGATGTA CTACACACAC 
CGGCGGCGGC GCTGGGTCCG TCTGCGCCGG AGGGACCTCA GCCAGATGGA GGCGTTGAAG 
CGGCACAGGC AGGCGGAGGC AGAGGGCGAG GGCTGGGAGT ACGCCTCGCT CTTCGGCTGG 
AAGTTCCATC TGGAGTACCG CAAGACAGAC GCCTTTCGTC GTCGCCGCTG GCGCCGCCGC 
ATGGAGCCCT TGGAGAAGAC AGGGCCTGCG GCTGTGTTTG CCCTTGAGGG GGCCCTGGGT 
GGCGTGGTGG ACGACAGGAG TGAAGATTCC GTGTCCGTCT CCACCTTGAG TTTTGGTGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT ACGCTGCTAC 
ATGTACCAGG CCCGGGATCT GCCTGCAATG GACAAGGACT CTTTTTCCGA TCCCTATGCA 
GTCGTCTCCT TCCTGCACCA AAGCCAGAAG ACGGTGGTGG CTAAGAACAC CCTAAACCCC 
ACCTGGGACC AGACGCTCAT CTTCTACGAG ATCGAGATCT TTGGCGAGCC AAGCAGCATC 
GCCGAGCAGC CTCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA TGGTGTAGAT 
GAGTTCATGG GACGTTGCAT CTGTCAGCCG AGTCTGGAAC GGACGCCTCG GCTGGCCTGG 
TTCCCGCTGA CGAGGGGCAG CCAGCCCGCC GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCTGCCAT CCACCATATT CCTGGTTTTG AGGTACAGGA CACAACAGGG 
ATCCTGGAAG AGTCAGAGGA CACGGACCTG CCCTACCCAC CGCCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACCGCCAT CGAGATCCTG 
GCGTGGGGTC TTCGGAACAT GAAGAGCTAC CAGCTGGCCA GCGTGTCCTC CCCGAGCCTG 
GTGGTGGAGT GCGGGGGCCA GTCTGTACAA TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTGTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCCGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGGGC TTCCTGTGTG ATCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGTGGCCC AGATGACGTG AGCTTACTCA GTCCTGGGGA AGATGTGCTG 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATCCAGG AGGAGGAGTT CATTGATTGG 
TGGAGCAAAT TCTTTGCTTC AATTGGGGAG AGCGAAAAGT GCGGGTCCTA CCTAGAGAAG 
GACTTTGACA CTCTAAAGGT CTATGACACA TCCCTGGAGA ATGTGAAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAATAC ATTCAAGCTC TACCGGGGCA AGACACAGGA GGAGACTGAA 
GACCCATCAG TCATTGGGGA GTTTAAGGGA CTCTTCAAAA TTTATCCTCT CCCAGAAGAC 
CCAGCCATCC CCTTGCCCCC GAGGCAGTTC CACCAGCTGG CTTCCCAGGG GCCCCAGGAA 
TGCCTGGTCC GCGTCTACAT TATCCGGGCG TTTGGCCTGC AGCCCAAGGA TCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CGGTGAGTGA CCAGGACAGC 
TACATCCCTT GTACTCTGGA GCCTGTATTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GGTCACCCTC TATGACTATG ACCTCCTCTC CAAGGATGAA 
AAGATTGGGG AGACGGTCAT TGACCTGGAG AACAGGCTGC TGTCTAAGTT CGGGGCCCGC 
TGTGGACTCC CCCAGACCTA CTGCGTCTCT GGACCCAACC AGTGGAGGGA TCAGCTCCGC 
CCCTCCCAGC TCCTCCACCT GTTCTGCCAG CAGCACCGAG TCAAGGCCCC CGTGTACCGG 
ACGGACCGCG TGGTGTTTCA GGATAAAGAA TACACCGTCG AAGAAATAGA GGCTGGCAGG 
GTCCCGAACC CACACCTGGG CCCAGTGGAG GAGCGTCTGG CCTTGCACGT CCTCCAGCAG 
CAGGGGCTGA TCCCTGAGCA CGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCGGAC 
ATCGAGCAGG GGAAGCTGCA GATGTGGGTC GACCTGTTTC CGAAGGCCCT GGGGCGGCCG 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACCA AGGATGTGAT CCTGGATGAC CTCAGCATCA CGGGGGAGAA GATGAGCGAC 
ATTTATGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTACAT 
TATCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCGTCTTCCC TTTTGACTAC 
CTGCCAGCTG AGCAGGTCTG CACCGTCTCC AAGAAGGATG CATTCTGGAG GCTGGACAAG 
ACGGAGAGCA AGATCCCTGC ACGAGTGGTA TTCCAGATCT GGGACAACGA CAAGTTCTCC 
TTCGATGACT TTCTGGGCTC TCTGCAGCTG GACCTTAACC ACATGCCCAA GCCCGCCAAG 
ACAGCTGAGA AGTGCTCTGC GGACCAGCTG GAAGACACCT TCCACCCAGA GCGGTTTGTG 
TCTCTTTTTG AACAGAAAAC AGTGAAGGGC TGGTGGCCCT GTGTGGCGGA AGAGGGTGAG 
AAGAAGATAC TGGCGGGCAA GCTGGAGATG ACCTTGGAGA TTGTAACAGA GAGTGAGCAT 
GAGGAGCGGC CAGCCGGCCA CGGCCGGGAT GAACCTAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGGC CTGACACCTC CTTCCTCTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG CTGTGCCATC ATCTTCTTCC TCATTCTCTT CATCTTCCTG 
CTCTTCTTGG GCATCTTTGT CTACTCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212496.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212553.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OBa23821
Clone ID Related Accession (Same CDS sequence)	XM_005212496.3
Accession Version	XM_005212496.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGT CAGAGGACAC GGACCTGCCC TACCCACCGC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CCGCCATCGA GATCCTGGCG 
TGGGGTCTTC GGAACATGAA GAGCTACCAG CTGGCCAGCG TGTCCTCCCC GAGCCTGGTG 
GTGGAGTGCG GGGGCCAGTC TGTACAATCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTGTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC CGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGGGCTTC CTGTGTGATC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GTGGCCCAGA TGACGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTGATT 
GACATCGATG ACAAGGAGCC CCTCATCCCC ATCCAGGAGG AGGAGTTCAT TGATTGGTGG 
AGCAAATTCT TTGCTTCAAT TGGGGAGAGC GAAAAGTGCG GGTCCTACCT AGAGAAGGAC 
TTTGACACTC TAAAGGTCTA TGACACATCC CTGGAGAATG TGAAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAATACATT CAAGCTCTAC CGGGGCAAGA CACAGGAGGA GACTGAAGAC 
CCATCAGTCA TTGGGGAGTT TAAGGGACTC TTCAAAATTT ATCCTCTCCC AGAAGACCCA 
GCCATCCCCT TGCCCCCGAG GCAGTTCCAC CAGCTGGCTT CCCAGGGGCC CCAGGAATGC 
CTGGTCCGCG TCTACATTAT CCGGGCGTTT GGCCTGCAGC CCAAGGATCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCGG TGAGTGACCA GGACAGCTAC 
ATCCCTTGTA CTCTGGAGCC TGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGGT CACCCTCTAT GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGGGAGA CGGTCATTGA CCTGGAGAAC AGGCTGCTGT CTAAGTTCGG GGCCCGCTGT 
GGACTCCCCC AGACCTACTG CGTCTCTGGA CCCAACCAGT GGAGGGATCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTGTT CTGCCAGCAG CACCGAGTCA AGGCCCCCGT GTACCGGACG 
GACCGCGTGG TGTTTCAGGA TAAAGAATAC ACCGTCGAAG AAATAGAGGC TGGCAGGGTC 
CCGAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCCT TGCACGTCCT CCAGCAGCAG 
GGGCTGATCC CTGAGCACGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCGGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGGCCGGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AACACCAAGG ATGTGATCCT GGATGACCTC AGCATCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGGT TGGCTTTGAA GAACACAAGC AAAAGACAGA TGTACATTAT 
CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCG TCTTCCCTTT TGACTACCTG 
CCAGCTGAGC AGGTCTGCAC CGTCTCCAAG AAGGATGCAT TCTGGAGGCT GGACAAGACG 
GAGAGCAAGA TCCCTGCACG AGTGGTATTC CAGATCTGGG ACAACGACAA GTTCTCCTTC 
GATGACTTTC TGGGCTCTCT GCAGCTGGAC CTTAACCACA TGCCCAAGCC CGCCAAGACA 
GCTGAGAAGT GCTCTGCGGA CCAGCTGGAA GACACCTTCC ACCCAGAGCG GTTTGTGTCT 
CTTTTTGAAC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TGGCGGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAGATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCAG CCGGCCACGG CCGGGATGAA CCTAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGGCCTG ACACCTCCTT CCTCTGGTTC ACCTCCCCGT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGCTG TGCCATCATC TTCTTCCTCA TTCTCTTCAT CTTCCTGCTC 
TTCTTGGGCA TCTTTGTCTA CTCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212553.1
CDS	538..6786
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212496.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212496.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTCCTCCAGC CACAGAACTG GACGACCATC TAGGCTTCCT CCCCACCTTC 
GGGCCCTGCT ACATCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGATCCC 
TATGCAGAGC TCAACACGGG CAAGGGAGAA GGCGTGGCCT ACCGAGGCCG GCTCCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCGCAGT GAACAGAAAG TGGAGGCTCT CTCTGCCGAT 
GACATCCTTC GGGTAGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCCGCCTTC 
TACTCGGCCA CCATGCTGCA GGATGTCGAT GACGCCGTCC AGTTCGAGGT CAGCATTGGG 
AACTATGGCA ACAAGTTCGA CACCACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGATGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CGTCTTACTG GGAGGACATC CGCCACAGAG TTGAGGCTCA GAACCAGCTT 
CTCAGGATTG CCGACCAGCT GGAAGCCGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCTCCG ACGAGGACGC GGACTCCCTG GTGGCTCAGC TGATGGATGA GCTCATCGCA 
GGCTGCAGCC AACCCCTGGG TGATGTCCAG GAGATGCCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACC AGCTGCGCAC CCGCCACCTG AGCCAGATCA CCGAGGCGGC CCAGGCCCTG 
AAGCTCGGCC ACAGTGAGCT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGG 
CTCCGTGCCC TGGCTGACGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTT 
CAGGGGGACA AGCGGGTGGC GTACCAGCGG GTGCCTGCCC ATGAGGTCCT CTTCTCCCGC 
CGGGGCACCA GCTACTGTGG CAAGAACTGT GGAAAACTGC AGACGATATT TCTGAAATAT 
CCGATGGAAG GGGTGCCCAG AGCCCGGATG CCGGTGCAGA TTCGGGTCAG GCTCTGGTTT 
GGACTCTCTG TGGATGAAAA GGAGTTCAAC CAGTTTGCTG AGGGCAAGCT CTCCGTCTTC 
GCTGAAACTT ATGAGAACCA GACCAAGCTG GCCCTGGTTG GGAACTGGGG CACAACTGGC 
CTCACCTACC CCAAGTTTTC TGACATCACG GGCAGGATCA AGCTACCCAA GGACAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACACTG 
CTTCACGACA CTGATGCTGG CCACCTGAGC TTCGTGGAGG AGGTATTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAGTGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTGAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCGCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCCGAGA AGATGTACTA CACACACCGG 
CGGCGGCGCT GGGTCCGTCT GCGCCGGAGG GACCTCAGCC AGATGGAGGC GTTGAAGCGG 
CACAGGCAGG CGGAGGCAGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT CGGCTGGAAG 
TTCCATCTGG AGTACCGCAA GACAGACGCC TTTCGTCGTC GCCGCTGGCG CCGCCGCATG 
GAGCCCTTGG AGAAGACAGG GCCTGCGGCT GTGTTTGCCC TTGAGGGGGC CCTGGGTGGC 
GTGGTGGACG ACAGGAGTGA AGATTCCGTG TCCGTCTCCA CCTTGAGTTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTACG CTGCTACATG 
TACCAGGCCC GGGATCTGCC TGCAATGGAC AAGGACTCTT TTTCCGATCC CTATGCAGTC 
GTCTCCTTCC TGCACCAAAG CCAGAAGACG GTGGTGGCTA AGAACACCCT AAACCCCACC 
TGGGACCAGA CGCTCATCTT CTACGAGATC GAGATCTTTG GCGAGCCAAG CAGCATCGCC 
GAGCAGCCTC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTATGG TGTAGATGAG 
TTCATGGGAC GTTGCATCTG TCAGCCGAGT CTGGAACGGA CGCCTCGGCT GGCCTGGTTC 
CCGCTGACGA GGGGCAGCCA GCCCGCCGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CTGCCATCCA CCATATTCCT GGTTTTGAGG TACAGGACAC AACAGGGATC 
CTGGAAGAGT CAGAGGACAC GGACCTGCCC TACCCACCGC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CCGCCATCGA GATCCTGGCG 
TGGGGTCTTC GGAACATGAA GAGCTACCAG CTGGCCAGCG TGTCCTCCCC GAGCCTGGTG 
GTGGAGTGCG GGGGCCAGTC TGTACAATCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTGTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC CGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGGGCTTC CTGTGTGATC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GTGGCCCAGA TGACGTGAGC TTACTCAGTC CTGGGGAAGA TGTGCTGATT 
GACATCGATG ACAAGGAGCC CCTCATCCCC ATCCAGGAGG AGGAGTTCAT TGATTGGTGG 
AGCAAATTCT TTGCTTCAAT TGGGGAGAGC GAAAAGTGCG GGTCCTACCT AGAGAAGGAC 
TTTGACACTC TAAAGGTCTA TGACACATCC CTGGAGAATG TGAAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAATACATT CAAGCTCTAC CGGGGCAAGA CACAGGAGGA GACTGAAGAC 
CCATCAGTCA TTGGGGAGTT TAAGGGACTC TTCAAAATTT ATCCTCTCCC AGAAGACCCA 
GCCATCCCCT TGCCCCCGAG GCAGTTCCAC CAGCTGGCTT CCCAGGGGCC CCAGGAATGC 
CTGGTCCGCG TCTACATTAT CCGGGCGTTT GGCCTGCAGC CCAAGGATCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCGG TGAGTGACCA GGACAGCTAC 
ATCCCTTGTA CTCTGGAGCC TGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGGT CACCCTCTAT GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGGGAGA CGGTCATTGA CCTGGAGAAC AGGCTGCTGT CTAAGTTCGG GGCCCGCTGT 
GGACTCCCCC AGACCTACTG CGTCTCTGGA CCCAACCAGT GGAGGGATCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTGTT CTGCCAGCAG CACCGAGTCA AGGCCCCCGT GTACCGGACG 
GACCGCGTGG TGTTTCAGGA TAAAGAATAC ACCGTCGAAG AAATAGAGGC TGGCAGGGTC 
CCGAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCCT TGCACGTCCT CCAGCAGCAG 
GGGCTGATCC CTGAGCACGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCGGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGGCCGGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AACACCAAGG ATGTGATCCT GGATGACCTC AGCATCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGGT TGGCTTTGAA GAACACAAGC AAAAGACAGA TGTACATTAT 
CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCG TCTTCCCTTT TGACTACCTG 
CCAGCTGAGC AGGTCTGCAC CGTCTCCAAG AAGGATGCAT TCTGGAGGCT GGACAAGACG 
GAGAGCAAGA TCCCTGCACG AGTGGTATTC CAGATCTGGG ACAACGACAA GTTCTCCTTC 
GATGACTTTC TGGGCTCTCT GCAGCTGGAC CTTAACCACA TGCCCAAGCC CGCCAAGACA 
GCTGAGAAGT GCTCTGCGGA CCAGCTGGAA GACACCTTCC ACCCAGAGCG GTTTGTGTCT 
CTTTTTGAAC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TGGCGGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAGATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCAG CCGGCCACGG CCGGGATGAA CCTAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGGCCTG ACACCTCCTT CCTCTGGTTC ACCTCCCCGT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGCTG TGCCATCATC TTCTTCCTCA TTCTCTTCAT CTTCCTGCTC 
TTCTTGGGCA TCTTTGTCTA CTCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212497.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212554.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OBa23822
Clone ID Related Accession (Same CDS sequence)	XM_005212497.3
Accession Version	XM_005212497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGT GGACGACCAT 
CTAGGCTTCC TCCCCACCTT CGGGCCCTGC TACATCAACC TCTATGGCAG CCCCAGGGAG 
TTCACTGGCT TCCCGGATCC CTATGCAGAG CTCAACACGG GCAAGGGAGA AGGCGTGGCC 
TACCGAGGCC GGCTCCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCGCAG TGAACAGAAA 
GTGGAGGCTC TCTCTGCCGA TGACATCCTT CGGGTAGAGA AGTACCTCCG GAGGCGCAAG 
TACTCGCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTGC AGGATGTCGA TGACGCCGTC 
CAGTTCGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACCACCTG CCTGCCGCTG 
GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGATGGAT GCCACTACTA CTACTTACCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCGTCTTACT GGGAGGACAT CCGCCACAGA 
GTTGAGGCTC AGAACCAGCT TCTCAGGATT GCCGACCAGC TGGAAGCCGG CCTGGAGCAA 
GTCCACTTGG CCCTGAAGGC TCAGTGCTCC GACGAGGACG CGGACTCCCT GGTGGCTCAG 
CTGATGGATG AGCTCATCGC AGGCTGCAGC CAACCCCTGG GTGATGTCCA GGAGATGCCC 
TCTGCCACCC ACCTGGACCA GTACCTGTAC CAGCTGCGCA CCCGCCACCT GAGCCAGATC 
ACCGAGGCGG CCCAGGCCCT GAAGCTCGGC CACAGTGAGC TCCCCGCTGC CCTGGAGCAG 
GCTGAGGACT GGCTCCTGCG GCTCCGTGCC CTGGCTGACG AGCCCCAGAA CAGCCTGCCT 
GACATCGTCA TCTGGATGCT TCAGGGGGAC AAGCGGGTGG CGTACCAGCG GGTGCCTGCC 
CATGAGGTCC TCTTCTCCCG CCGGGGCACC AGCTACTGTG GCAAGAACTG TGGAAAACTG 
CAGACGATAT TTCTGAAATA TCCGATGGAA GGGGTGCCCA GAGCCCGGAT GCCGGTGCAG 
ATTCGGGTCA GGCTCTGGTT TGGACTCTCT GTGGATGAAA AGGAGTTCAA CCAGTTTGCT 
GAGGGCAAGC TCTCCGTCTT CGCTGAAACT TATGAGAACC AGACCAAGCT GGCCCTGGTT 
GGGAACTGGG GCACAACTGG CCTCACCTAC CCCAAGTTTT CTGACATCAC GGGCAGGATC 
AAGCTACCCA AGGACAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCTGG AGACTGGTTC 
GTGTGTCCAG AGAAGACACT GCTTCACGAC ACTGATGCTG GCCACCTGAG CTTCGTGGAG 
GAGGTATTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACGGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAGTG CCCGCTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTGAATC GGGCTGTCGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT CCCGCCGGAC CGGAAGCCGA GGCACTGGGT CCCTGCCGAG 
AAGATGTACT ACACACACCG GCGGCGGCGC TGGGTCCGTC TGCGCCGGAG GGACCTCAGC 
CAGATGGAGG CGTTGAAGCG GCACAGGCAG GCGGAGGCAG AGGGCGAGGG CTGGGAGTAC 
GCCTCGCTCT TCGGCTGGAA GTTCCATCTG GAGTACCGCA AGACAGACGC CTTTCGTCGT 
CGCCGCTGGC GCCGCCGCAT GGAGCCCTTG GAGAAGACAG GGCCTGCGGC TGTGTTTGCC 
CTTGAGGGGG CCCTGGGTGG CGTGGTGGAC GACAGGAGTG AAGATTCCGT GTCCGTCTCC 
ACCTTGAGTT TTGGTGTGAA CAGACCCACG ATTTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTAC GCTGCTACAT GTACCAGGCC CGGGATCTGC CTGCAATGGA CAAGGACTCT 
TTTTCCGATC CCTATGCAGT CGTCTCCTTC CTGCACCAAA GCCAGAAGAC GGTGGTGGCT 
AAGAACACCC TAAACCCCAC CTGGGACCAG ACGCTCATCT TCTACGAGAT CGAGATCTTT 
GGCGAGCCAA GCAGCATCGC CGAGCAGCCT CCCAGCATCG TGGTGGAGCT GTATGACCAT 
GACACCTATG GTGTAGATGA GTTCATGGGA CGTTGCATCT GTCAGCCGAG TCTGGAACGG 
ACGCCTCGGC TGGCCTGGTT CCCGCTGACG AGGGGCAGCC AGCCCGCCGG TGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCTGCCATCC ACCATATTCC TGGTTTTGAG 
GTACAGGACA CAACAGGGAT CCTGGAAGAG TCAGAGGACA CGGACCTGCC CTACCCACCG 
CCCCAGAGGG AGGCCAACAT CTACATGGTC CCCCAGAACA TCAAGCCGGT TCTCCAGCGA 
ACCGCCATCG AGATCCTGGC GTGGGGTCTT CGGAACATGA AGAGCTACCA GCTGGCCAGC 
GTGTCCTCCC CGAGCCTGGT GGTGGAGTGC GGGGGCCAGT CTGTACAATC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCAGGGAGG AGCTGTACTG TCCCCCCATC GTGGTCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CCGTGGTGGG CCAGTGTACC ATCCGCTCCC TGGAGGGCTT CCTGTGTGAT 
CCTTACTCAG AGGAGAGTCC GTCCCCACAG GGTGGCCCAG ATGACGTGAG CTTACTCAGT 
CCTGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATCCAGGAG 
GAGGAGTTCA TTGATTGGTG GAGCAAATTC TTTGCTTCAA TTGGGGAGAG CGAAAAGTGC 
GGGTCCTACC TAGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACATC CCTGGAGAAT 
GTGAAGGCCT TTGAGGGCCT GTCTGACTTT TGTAATACAT TCAAGCTCTA CCGGGGCAAG 
ACACAGGAGG AGACTGAAGA CCCATCAGTC ATTGGGGAGT TTAAGGGACT CTTCAAAATT 
TATCCTCTCC CAGAAGACCC AGCCATCCCC TTGCCCCCGA GGCAGTTCCA CCAGCTGGCT 
TCCCAGGGGC CCCAGGAATG CCTGGTCCGC GTCTACATTA TCCGGGCGTT TGGCCTGCAG 
CCCAAGGATC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGTGACC AGGACAGCTA CATCCCTTGT ACTCTGGAGC CTGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGG TCACCCTCTA TGACTATGAC 
CTCCTCTCCA AGGATGAAAA GATTGGGGAG ACGGTCATTG ACCTGGAGAA CAGGCTGCTG 
TCTAAGTTCG GGGCCCGCTG TGGACTCCCC CAGACCTACT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTGT TCTGCCAGCA GCACCGAGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCGTCGAA 
GAAATAGAGG CTGGCAGGGT CCCGAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCA GGGGCTGATC CCTGAGCACG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATCC TGGATGACCT CAGCATCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ATGTACATTA TCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
GTCTTCCCTT TTGACTACCT GCCAGCTGAG CAGGTCTGCA CCGTCTCCAA GAAGGATGCA 
TTCTGGAGGC TGGACAAGAC GGAGAGCAAG ATCCCTGCAC GAGTGGTATT CCAGATCTGG 
GACAACGACA AGTTCTCCTT CGATGACTTT CTGGGCTCTC TGCAGCTGGA CCTTAACCAC 
ATGCCCAAGC CCGCCAAGAC AGCTGAGAAG TGCTCTGCGG ACCAGCTGGA AGACACCTTC 
CACCCAGAGC GGTTTGTGTC TCTTTTTGAA CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTGGCGGAAG AGGGTGAGAA GAAGATACTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT 
GTAACAGAGA GTGAGCATGA GGAGCGGCCA GCCGGCCACG GCCGGGATGA ACCTAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGGCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGCT GTGCCATCAT CTTCTTCCTC 
ATTCTCTTCA TCTTCCTGCT CTTCTTGGGC ATCTTTGTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212554.1
CDS	129..6338
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212497.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212497.3

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC GGGAGACGAG GCCGAGCCAT TCCTGGACCA GAGCGGGGCT 
CTGGGCCCGG GGGCCCCCAG CACCCCGAAG AAACAACCTT CCCATCCGCC CCCCTACCAC 
CCCGGAGGGG GAAGGAAGAG AAGCGCGCCT GCGCCCAGCA AGCCTCTGTC GGACAAGCCG 
CAGGACTTCC AGATCAGGGT CCAGGTGATC AAGGGACGCC AGCTGCCAGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCCGCTGGG CAGACCAAGC GGACGCGGAT CCACAAAGGA 
AACAGCCCCG TCTTCAATGA GACTCTCTTC TTCAACGTGT TTGACTCTCC CGCGGAGCTG 
TTCAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGAAC AGATGCTCTC 
ATCGGGGAGT TCCGGCTGGA CGTGGGCAGC ATCTACCGAG AGCCCCGACA TGCCTACCTC 
AGGAAGTGGC TGCTGCTCTC AGATCCCGAC GATTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCGAGTC TCTGTGTGCT GGGGCCTGGA GATGAAGCCC CCCTGGAGAG AAAAGACCCC 
TCTGAAGACA AGGAGGACAT CGAAAGCAAC CTGCTTAGGC CGATCGGCGT GGCCCTCCGT 
GGAGCGCACT TCTGTCTGAA GGTCTTCAGG GCCGAGGACT TGCCACAGAT GGACGATGCC 
GTGATGGACA ACGTGAGGCA GATCTTCGGC TTTGACAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTTGTGG AAGTCAGCTT TGCAGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACG 
GCCAATCCTC AGTGGAACCA GAGCATCACA CTGCCTGCCA TGTTTCCATC CATGTGTGAG 
AAAATGAGGA TTCGTGTCGT AGACTGGGAC CGCCTCACCC ACAACGATAT TGTGGCCACC 
ACCTACCTGA GTATGTCGAA GATCTCTGCC TCTGGAGGCG AGATAGAAGT GGACGACCAT 
CTAGGCTTCC TCCCCACCTT CGGGCCCTGC TACATCAACC TCTATGGCAG CCCCAGGGAG 
TTCACTGGCT TCCCGGATCC CTATGCAGAG CTCAACACGG GCAAGGGAGA AGGCGTGGCC 
TACCGAGGCC GGCTCCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCGCAG TGAACAGAAA 
GTGGAGGCTC TCTCTGCCGA TGACATCCTT CGGGTAGAGA AGTACCTCCG GAGGCGCAAG 
TACTCGCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTGC AGGATGTCGA TGACGCCGTC 
CAGTTCGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACCACCTG CCTGCCGCTG 
GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGATGGAT GCCACTACTA CTACTTACCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCGTCTTACT GGGAGGACAT CCGCCACAGA 
GTTGAGGCTC AGAACCAGCT TCTCAGGATT GCCGACCAGC TGGAAGCCGG CCTGGAGCAA 
GTCCACTTGG CCCTGAAGGC TCAGTGCTCC GACGAGGACG CGGACTCCCT GGTGGCTCAG 
CTGATGGATG AGCTCATCGC AGGCTGCAGC CAACCCCTGG GTGATGTCCA GGAGATGCCC 
TCTGCCACCC ACCTGGACCA GTACCTGTAC CAGCTGCGCA CCCGCCACCT GAGCCAGATC 
ACCGAGGCGG CCCAGGCCCT GAAGCTCGGC CACAGTGAGC TCCCCGCTGC CCTGGAGCAG 
GCTGAGGACT GGCTCCTGCG GCTCCGTGCC CTGGCTGACG AGCCCCAGAA CAGCCTGCCT 
GACATCGTCA TCTGGATGCT TCAGGGGGAC AAGCGGGTGG CGTACCAGCG GGTGCCTGCC 
CATGAGGTCC TCTTCTCCCG CCGGGGCACC AGCTACTGTG GCAAGAACTG TGGAAAACTG 
CAGACGATAT TTCTGAAATA TCCGATGGAA GGGGTGCCCA GAGCCCGGAT GCCGGTGCAG 
ATTCGGGTCA GGCTCTGGTT TGGACTCTCT GTGGATGAAA AGGAGTTCAA CCAGTTTGCT 
GAGGGCAAGC TCTCCGTCTT CGCTGAAACT TATGAGAACC AGACCAAGCT GGCCCTGGTT 
GGGAACTGGG GCACAACTGG CCTCACCTAC CCCAAGTTTT CTGACATCAC GGGCAGGATC 
AAGCTACCCA AGGACAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCTGG AGACTGGTTC 
GTGTGTCCAG AGAAGACACT GCTTCACGAC ACTGATGCTG GCCACCTGAG CTTCGTGGAG 
GAGGTATTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACGGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAGTG CCCGCTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTGAATC GGGCTGTCGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT CCCGCCGGAC CGGAAGCCGA GGCACTGGGT CCCTGCCGAG 
AAGATGTACT ACACACACCG GCGGCGGCGC TGGGTCCGTC TGCGCCGGAG GGACCTCAGC 
CAGATGGAGG CGTTGAAGCG GCACAGGCAG GCGGAGGCAG AGGGCGAGGG CTGGGAGTAC 
GCCTCGCTCT TCGGCTGGAA GTTCCATCTG GAGTACCGCA AGACAGACGC CTTTCGTCGT 
CGCCGCTGGC GCCGCCGCAT GGAGCCCTTG GAGAAGACAG GGCCTGCGGC TGTGTTTGCC 
CTTGAGGGGG CCCTGGGTGG CGTGGTGGAC GACAGGAGTG AAGATTCCGT GTCCGTCTCC 
ACCTTGAGTT TTGGTGTGAA CAGACCCACG ATTTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTAC GCTGCTACAT GTACCAGGCC CGGGATCTGC CTGCAATGGA CAAGGACTCT 
TTTTCCGATC CCTATGCAGT CGTCTCCTTC CTGCACCAAA GCCAGAAGAC GGTGGTGGCT 
AAGAACACCC TAAACCCCAC CTGGGACCAG ACGCTCATCT TCTACGAGAT CGAGATCTTT 
GGCGAGCCAA GCAGCATCGC CGAGCAGCCT CCCAGCATCG TGGTGGAGCT GTATGACCAT 
GACACCTATG GTGTAGATGA GTTCATGGGA CGTTGCATCT GTCAGCCGAG TCTGGAACGG 
ACGCCTCGGC TGGCCTGGTT CCCGCTGACG AGGGGCAGCC AGCCCGCCGG TGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCTGCCATCC ACCATATTCC TGGTTTTGAG 
GTACAGGACA CAACAGGGAT CCTGGAAGAG TCAGAGGACA CGGACCTGCC CTACCCACCG 
CCCCAGAGGG AGGCCAACAT CTACATGGTC CCCCAGAACA TCAAGCCGGT TCTCCAGCGA 
ACCGCCATCG AGATCCTGGC GTGGGGTCTT CGGAACATGA AGAGCTACCA GCTGGCCAGC 
GTGTCCTCCC CGAGCCTGGT GGTGGAGTGC GGGGGCCAGT CTGTACAATC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCAGGGAGG AGCTGTACTG TCCCCCCATC GTGGTCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CCGTGGTGGG CCAGTGTACC ATCCGCTCCC TGGAGGGCTT CCTGTGTGAT 
CCTTACTCAG AGGAGAGTCC GTCCCCACAG GGTGGCCCAG ATGACGTGAG CTTACTCAGT 
CCTGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATCCAGGAG 
GAGGAGTTCA TTGATTGGTG GAGCAAATTC TTTGCTTCAA TTGGGGAGAG CGAAAAGTGC 
GGGTCCTACC TAGAGAAGGA CTTTGACACT CTAAAGGTCT ATGACACATC CCTGGAGAAT 
GTGAAGGCCT TTGAGGGCCT GTCTGACTTT TGTAATACAT TCAAGCTCTA CCGGGGCAAG 
ACACAGGAGG AGACTGAAGA CCCATCAGTC ATTGGGGAGT TTAAGGGACT CTTCAAAATT 
TATCCTCTCC CAGAAGACCC AGCCATCCCC TTGCCCCCGA GGCAGTTCCA CCAGCTGGCT 
TCCCAGGGGC CCCAGGAATG CCTGGTCCGC GTCTACATTA TCCGGGCGTT TGGCCTGCAG 
CCCAAGGATC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGTGACC AGGACAGCTA CATCCCTTGT ACTCTGGAGC CTGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGG TCACCCTCTA TGACTATGAC 
CTCCTCTCCA AGGATGAAAA GATTGGGGAG ACGGTCATTG ACCTGGAGAA CAGGCTGCTG 
TCTAAGTTCG GGGCCCGCTG TGGACTCCCC CAGACCTACT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTGT TCTGCCAGCA GCACCGAGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCGTCGAA 
GAAATAGAGG CTGGCAGGGT CCCGAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCC 
TTGCACGTCC TCCAGCAGCA GGGGCTGATC CCTGAGCACG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATCC TGGATGACCT CAGCATCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ATGTACATTA TCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
GTCTTCCCTT TTGACTACCT GCCAGCTGAG CAGGTCTGCA CCGTCTCCAA GAAGGATGCA 
TTCTGGAGGC TGGACAAGAC GGAGAGCAAG ATCCCTGCAC GAGTGGTATT CCAGATCTGG 
GACAACGACA AGTTCTCCTT CGATGACTTT CTGGGCTCTC TGCAGCTGGA CCTTAACCAC 
ATGCCCAAGC CCGCCAAGAC AGCTGAGAAG TGCTCTGCGG ACCAGCTGGA AGACACCTTC 
CACCCAGAGC GGTTTGTGTC TCTTTTTGAA CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTGGCGGAAG AGGGTGAGAA GAAGATACTG GCGGGCAAGC TGGAGATGAC CTTGGAGATT 
GTAACAGAGA GTGAGCATGA GGAGCGGCCA GCCGGCCACG GCCGGGATGA ACCTAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGGCCT GACACCTCCT TCCTCTGGTT CACCTCCCCG 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGGTTCCGCT GTGCCATCAT CTTCTTCCTC 
ATTCTCTTCA TCTTCCTGCT CTTCTTGGGC ATCTTTGTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005212498.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212555.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OBa23823
Clone ID Related Accession (Same CDS sequence)	XM_005212498.3
Accession Version	XM_005212498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGTGGA CGACCATCTA 
GGCTTCCTCC CCACCTTCGG GCCCTGCTAC ATCAACCTCT ATGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGATCCCTA TGCAGAGCTC AACACGGGCA AGGGAGAAGG CGTGGCCTAC 
CGAGGCCGGC TCCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCGCAGTGA ACAGAAAGTG 
GAGGCTCTCT CTGCCGATGA CATCCTTCGG GTAGAGAAGT ACCTCCGGAG GCGCAAGTAC 
TCGCTCTTTG CCGCCTTCTA CTCGGCCACC ATGCTGCAGG ATGTCGATGA CGCCGTCCAG 
TTCGAGGTCA GCATTGGGAA CTATGGCAAC AAGTTCGACA CCACCTGCCT GCCGCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTACTA CTTACCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCG TCTTACTGGG AGGACATCCG CCACAGAGTT 
GAGGCTCAGA ACCAGCTTCT CAGGATTGCC GACCAGCTGG AAGCCGGCCT GGAGCAAGTC 
CACTTGGCCC TGAAGGCTCA GTGCTCCGAC GAGGACGCGG ACTCCCTGGT GGCTCAGCTG 
ATGGATGAGC TCATCGCAGG CTGCAGCCAA CCCCTGGGTG ATGTCCAGGA GATGCCCTCT 
GCCACCCACC TGGACCAGTA CCTGTACCAG CTGCGCACCC GCCACCTGAG CCAGATCACC 
GAGGCGGCCC AGGCCCTGAA GCTCGGCCAC AGTGAGCTCC CCGCTGCCCT GGAGCAGGCT 
GAGGACTGGC TCCTGCGGCT CCGTGCCCTG GCTGACGAGC CCCAGAACAG CCTGCCTGAC 
ATCGTCATCT GGATGCTTCA GGGGGACAAG CGGGTGGCGT ACCAGCGGGT GCCTGCCCAT 
GAGGTCCTCT TCTCCCGCCG GGGCACCAGC TACTGTGGCA AGAACTGTGG AAAACTGCAG 
ACGATATTTC TGAAATATCC GATGGAAGGG GTGCCCAGAG CCCGGATGCC GGTGCAGATT 
CGGGTCAGGC TCTGGTTTGG ACTCTCTGTG GATGAAAAGG AGTTCAACCA GTTTGCTGAG 
GGCAAGCTCT CCGTCTTCGC TGAAACTTAT GAGAACCAGA CCAAGCTGGC CCTGGTTGGG 
AACTGGGGCA CAACTGGCCT CACCTACCCC AAGTTTTCTG ACATCACGGG CAGGATCAAG 
CTACCCAAGG ACAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCTGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACACTGCT TCACGACACT GATGCTGGCC ACCTGAGCTT CGTGGAGGAG 
GTATTTGAGA ACCAGACCCG GCTCCCTGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAGTGCCC GCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTGAATCGGG CTGTCGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATCCC GCCGGACCGG AAGCCGAGGC ACTGGGTCCC TGCCGAGAAG 
ATGTACTACA CACACCGGCG GCGGCGCTGG GTCCGTCTGC GCCGGAGGGA CCTCAGCCAG 
ATGGAGGCGT TGAAGCGGCA CAGGCAGGCG GAGGCAGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTCG GCTGGAAGTT CCATCTGGAG TACCGCAAGA CAGACGCCTT TCGTCGTCGC 
CGCTGGCGCC GCCGCATGGA GCCCTTGGAG AAGACAGGGC CTGCGGCTGT GTTTGCCCTT 
GAGGGGGCCC TGGGTGGCGT GGTGGACGAC AGGAGTGAAG ATTCCGTGTC CGTCTCCACC 
TTGAGTTTTG GTGTGAACAG ACCCACGATT TCCTGCATCT TTGACTATGG GAACCGCTAC 
CATCTACGCT GCTACATGTA CCAGGCCCGG GATCTGCCTG CAATGGACAA GGACTCTTTT 
TCCGATCCCT ATGCAGTCGT CTCCTTCCTG CACCAAAGCC AGAAGACGGT GGTGGCTAAG 
AACACCCTAA ACCCCACCTG GGACCAGACG CTCATCTTCT ACGAGATCGA GATCTTTGGC 
GAGCCAAGCA GCATCGCCGA GCAGCCTCCC AGCATCGTGG TGGAGCTGTA TGACCATGAC 
ACCTATGGTG TAGATGAGTT CATGGGACGT TGCATCTGTC AGCCGAGTCT GGAACGGACG 
CCTCGGCTGG CCTGGTTCCC GCTGACGAGG GGCAGCCAGC CCGCCGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCT GCCATCCACC ATATTCCTGG TTTTGAGGTA 
CAGGACACAA CAGGGATCCT GGAAGAGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGGAGGAG 
GAGTTCATTG ATTGGTGGAG CAAATTCTTT GCTTCAATTG GGGAGAGCGA AAAGTGCGGG 
TCCTACCTAG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACATCCCT GGAGAATGTG 
AAGGCCTTTG AGGGCCTGTC TGACTTTTGT AATACATTCA AGCTCTACCG GGGCAAGACA 
CAGGAGGAGA CTGAAGACCC ATCAGTCATT GGGGAGTTTA AGGGACTCTT CAAAATTTAT 
CCTCTCCCAG AAGACCCAGC CATCCCCTTG CCCCCGAGGC AGTTCCACCA GCTGGCTTCC 
CAGGGGCCCC AGGAATGCCT GGTCCGCGTC TACATTATCC GGGCGTTTGG CCTGCAGCCC 
AAGGATCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCGGTG 
AGTGACCAGG ACAGCTACAT CCCTTGTACT CTGGAGCCTG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CCCTCTATGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGGGAGACG GTCATTGACC TGGAGAACAG GCTGCTGTCT 
AAGTTCGGGG CCCGCTGTGG ACTCCCCCAG ACCTACTGCG TCTCTGGACC CAACCAGTGG 
AGGGATCAGC TCCGCCCCTC CCAGCTCCTC CACCTGTTCT GCCAGCAGCA CCGAGTCAAG 
GCCCCCGTGT ACCGGACGGA CCGCGTGGTG TTTCAGGATA AAGAATACAC CGTCGAAGAA 
ATAGAGGCTG GCAGGGTCCC GAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCAGGG GCTGATCCCT GAGCACGTGG AGTCCCGGCC TCTCTACAGC 
CCCCTGCAGC CGGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GGCCGGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACCAAGGAT GTGATCCTGG ATGACCTCAG CATCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TACATTATCG GTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCGTC 
TTCCCTTTTG ACTACCTGCC AGCTGAGCAG GTCTGCACCG TCTCCAAGAA GGATGCATTC 
TGGAGGCTGG ACAAGACGGA GAGCAAGATC CCTGCACGAG TGGTATTCCA GATCTGGGAC 
AACGACAAGT TCTCCTTCGA TGACTTTCTG GGCTCTCTGC AGCTGGACCT TAACCACATG 
CCCAAGCCCG CCAAGACAGC TGAGAAGTGC TCTGCGGACC AGCTGGAAGA CACCTTCCAC 
CCAGAGCGGT TTGTGTCTCT TTTTGAACAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTG 
GCGGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCAGCC GGCCACGGCC GGGATGAACC TAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGGCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGCTGTG CCATCATCTT CTTCCTCATT 
CTCTTCATCT TCCTGCTCTT CTTGGGCATC TTTGTCTACT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212555.1
CDS	538..6744
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVADTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005212498.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212498.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AATGTCCACA CCCCTGACAC CGACATCAGC 
GATGCCTACT GCTCCGCAGT GTTTGCAGGT GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AATGTGAACC CTGTGTGGAA TGAGGGATTC GAGTGGGACC TCAAGGGTAT CCCCCTGGAC 
CAGGGCTCCG AGCTTCTTGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACATCCC CCTCCGGGAA GTCCTCGCCA CCCCCAGCCT GTCTGCCAGC 
TTCAATGCCC CACTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCGCTGCC TGGAGCCGTG CCCACCTTCC CACCCTTAAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATACG GTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACGGG AGACGAGGCC GAGCCATTCC TGGACCAGAG CGGGGCTCTG 
GGCCCGGGGG CCCCCAGCAC CCCGAAGAAA CAACCTTCCC ATCCGCCCCC CTACCACCCC 
GGAGGGGGAA GGAAGAGAAG CGCGCCTGCG CCCAGCAAGC CTCTGTCGGA CAAGCCGCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCAAG GGACGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC CGCTGGGCAG ACCAAGCGGA CGCGGATCCA CAAAGGAAAC 
AGCCCCGTCT TCAATGAGAC TCTCTTCTTC AACGTGTTTG ACTCTCCCGC GGAGCTGTTC 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGAACAGA TGCTCTCATC 
GGGGAGTTCC GGCTGGACGT GGGCAGCATC TACCGAGAGC CCCGACATGC CTACCTCAGG 
AAGTGGCTGC TGCTCTCAGA TCCCGACGAT TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCGAGTCTCT GTGTGCTGGG GCCTGGAGAT GAAGCCCCCC TGGAGAGAAA AGACCCCTCT 
GAAGACAAGG AGGACATCGA AAGCAACCTG CTTAGGCCGA TCGGCGTGGC CCTCCGTGGA 
GCGCACTTCT GTCTGAAGGT CTTCAGGGCC GAGGACTTGC CACAGATGGA CGATGCCGTG 
ATGGACAACG TGAGGCAGAT CTTCGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTTGTGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACGGCC 
AATCCTCAGT GGAACCAGAG CATCACACTG CCTGCCATGT TTCCATCCAT GTGTGAGAAA 
ATGAGGATTC GTGTCGTAGA CTGGGACCGC CTCACCCACA ACGATATTGT GGCCACCACC 
TACCTGAGTA TGTCGAAGAT CTCTGCCTCT GGAGGCGAGA TAGAAGTGGA CGACCATCTA 
GGCTTCCTCC CCACCTTCGG GCCCTGCTAC ATCAACCTCT ATGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGATCCCTA TGCAGAGCTC AACACGGGCA AGGGAGAAGG CGTGGCCTAC 
CGAGGCCGGC TCCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCGCAGTGA ACAGAAAGTG 
GAGGCTCTCT CTGCCGATGA CATCCTTCGG GTAGAGAAGT ACCTCCGGAG GCGCAAGTAC 
TCGCTCTTTG CCGCCTTCTA CTCGGCCACC ATGCTGCAGG ATGTCGATGA CGCCGTCCAG 
TTCGAGGTCA GCATTGGGAA CTATGGCAAC AAGTTCGACA CCACCTGCCT GCCGCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTACTA CTTACCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCG TCTTACTGGG AGGACATCCG CCACAGAGTT 
GAGGCTCAGA ACCAGCTTCT CAGGATTGCC GACCAGCTGG AAGCCGGCCT GGAGCAAGTC 
CACTTGGCCC TGAAGGCTCA GTGCTCCGAC GAGGACGCGG ACTCCCTGGT GGCTCAGCTG 
ATGGATGAGC TCATCGCAGG CTGCAGCCAA CCCCTGGGTG ATGTCCAGGA GATGCCCTCT 
GCCACCCACC TGGACCAGTA CCTGTACCAG CTGCGCACCC GCCACCTGAG CCAGATCACC 
GAGGCGGCCC AGGCCCTGAA GCTCGGCCAC AGTGAGCTCC CCGCTGCCCT GGAGCAGGCT 
GAGGACTGGC TCCTGCGGCT CCGTGCCCTG GCTGACGAGC CCCAGAACAG CCTGCCTGAC 
ATCGTCATCT GGATGCTTCA GGGGGACAAG CGGGTGGCGT ACCAGCGGGT GCCTGCCCAT 
GAGGTCCTCT TCTCCCGCCG GGGCACCAGC TACTGTGGCA AGAACTGTGG AAAACTGCAG 
ACGATATTTC TGAAATATCC GATGGAAGGG GTGCCCAGAG CCCGGATGCC GGTGCAGATT 
CGGGTCAGGC TCTGGTTTGG ACTCTCTGTG GATGAAAAGG AGTTCAACCA GTTTGCTGAG 
GGCAAGCTCT CCGTCTTCGC TGAAACTTAT GAGAACCAGA CCAAGCTGGC CCTGGTTGGG 
AACTGGGGCA CAACTGGCCT CACCTACCCC AAGTTTTCTG ACATCACGGG CAGGATCAAG 
CTACCCAAGG ACAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCTGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACACTGCT TCACGACACT GATGCTGGCC ACCTGAGCTT CGTGGAGGAG 
GTATTTGAGA ACCAGACCCG GCTCCCTGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAGTGCCC GCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTGAATCGGG CTGTCGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATCCC GCCGGACCGG AAGCCGAGGC ACTGGGTCCC TGCCGAGAAG 
ATGTACTACA CACACCGGCG GCGGCGCTGG GTCCGTCTGC GCCGGAGGGA CCTCAGCCAG 
ATGGAGGCGT TGAAGCGGCA CAGGCAGGCG GAGGCAGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTCG GCTGGAAGTT CCATCTGGAG TACCGCAAGA CAGACGCCTT TCGTCGTCGC 
CGCTGGCGCC GCCGCATGGA GCCCTTGGAG AAGACAGGGC CTGCGGCTGT GTTTGCCCTT 
GAGGGGGCCC TGGGTGGCGT GGTGGACGAC AGGAGTGAAG ATTCCGTGTC CGTCTCCACC 
TTGAGTTTTG GTGTGAACAG ACCCACGATT TCCTGCATCT TTGACTATGG GAACCGCTAC 
CATCTACGCT GCTACATGTA CCAGGCCCGG GATCTGCCTG CAATGGACAA GGACTCTTTT 
TCCGATCCCT ATGCAGTCGT CTCCTTCCTG CACCAAAGCC AGAAGACGGT GGTGGCTAAG 
AACACCCTAA ACCCCACCTG GGACCAGACG CTCATCTTCT ACGAGATCGA GATCTTTGGC 
GAGCCAAGCA GCATCGCCGA GCAGCCTCCC AGCATCGTGG TGGAGCTGTA TGACCATGAC 
ACCTATGGTG TAGATGAGTT CATGGGACGT TGCATCTGTC AGCCGAGTCT GGAACGGACG 
CCTCGGCTGG CCTGGTTCCC GCTGACGAGG GGCAGCCAGC CCGCCGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCT GCCATCCACC ATATTCCTGG TTTTGAGGTA 
CAGGACACAA CAGGGATCCT GGAAGAGTCA GAGGACACGG ACCTGCCCTA CCCACCGCCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACC 
GCCATCGAGA TCCTGGCGTG GGGTCTTCGG AACATGAAGA GCTACCAGCT GGCCAGCGTG 
TCCTCCCCGA GCCTGGTGGT GGAGTGCGGG GGCCAGTCTG TACAATCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TGTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCCG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGGGCTTCCT GTGTGATCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGT GGCCCAGATG ACGTGAGCTT ACTCAGTCCT 
GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT CCAGGAGGAG 
GAGTTCATTG ATTGGTGGAG CAAATTCTTT GCTTCAATTG GGGAGAGCGA AAAGTGCGGG 
TCCTACCTAG AGAAGGACTT TGACACTCTA AAGGTCTATG ACACATCCCT GGAGAATGTG 
AAGGCCTTTG AGGGCCTGTC TGACTTTTGT AATACATTCA AGCTCTACCG GGGCAAGACA 
CAGGAGGAGA CTGAAGACCC ATCAGTCATT GGGGAGTTTA AGGGACTCTT CAAAATTTAT 
CCTCTCCCAG AAGACCCAGC CATCCCCTTG CCCCCGAGGC AGTTCCACCA GCTGGCTTCC 
CAGGGGCCCC AGGAATGCCT GGTCCGCGTC TACATTATCC GGGCGTTTGG CCTGCAGCCC 
AAGGATCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCGGTG 
AGTGACCAGG ACAGCTACAT CCCTTGTACT CTGGAGCCTG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CCCTCTATGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGGGAGACG GTCATTGACC TGGAGAACAG GCTGCTGTCT 
AAGTTCGGGG CCCGCTGTGG ACTCCCCCAG ACCTACTGCG TCTCTGGACC CAACCAGTGG 
AGGGATCAGC TCCGCCCCTC CCAGCTCCTC CACCTGTTCT GCCAGCAGCA CCGAGTCAAG 
GCCCCCGTGT ACCGGACGGA CCGCGTGGTG TTTCAGGATA AAGAATACAC CGTCGAAGAA 
ATAGAGGCTG GCAGGGTCCC GAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCCTTG 
CACGTCCTCC AGCAGCAGGG GCTGATCCCT GAGCACGTGG AGTCCCGGCC TCTCTACAGC 
CCCCTGCAGC CGGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GGCCGGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACCAAGGAT GTGATCCTGG ATGACCTCAG CATCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TACATTATCG GTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCGTC 
TTCCCTTTTG ACTACCTGCC AGCTGAGCAG GTCTGCACCG TCTCCAAGAA GGATGCATTC 
TGGAGGCTGG ACAAGACGGA GAGCAAGATC CCTGCACGAG TGGTATTCCA GATCTGGGAC 
AACGACAAGT TCTCCTTCGA TGACTTTCTG GGCTCTCTGC AGCTGGACCT TAACCACATG 
CCCAAGCCCG CCAAGACAGC TGAGAAGTGC TCTGCGGACC AGCTGGAAGA CACCTTCCAC 
CCAGAGCGGT TTGTGTCTCT TTTTGAACAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTG 
GCGGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AGATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCAGCC GGCCACGGCC GGGATGAACC TAACATGAAC 
CCCAAGCTCG AGGACCCAAG GCGGCCTGAC ACCTCCTTCC TCTGGTTCAC CTCCCCGTAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGG TTCCGCTGTG CCATCATCTT CTTCCTCATT 
CTCTTCATCT TCCTGCTCTT CTTGGGCATC TTTGTCTACT CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_010809847.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808149.1 Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa47068
Clone ID Related Accession (Same CDS sequence)	XM_010809847.2
Accession Version	XM_010809847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104290.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGTCAGA GGACACGGAC 
CTGCCCTACC CACCGCCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACCGC CATCGAGATC CTGGCGTGGG GTCTTCGGAA CATGAAGAGC 
TACCAGCTGG CCAGCGTGTC CTCCCCGAGC CTGGTGGTGG AGTGCGGGGG CCAGTCTGTA 
CAATCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTG TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCCGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
GGCTTCCTGT GTGATCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATCC AGTTTGCAGA TGGTCTCTCG GGCTTGGCCC CCACTAACAT GGCATCTTCT 
CCATCCAGTC TTCATGAGGA GGAGTTCATT GATTGGTGGA GCAAATTCTT TGCTTCAATT 
GGGGAGAGCG AAAAGTGCGG GTCCTACCTA GAGAAGGACT TTGACACTCT AAAGGTCTAT 
GACACATCCC TGGAGAATGT GAAGGCCTTT GAGGGCCTGT CTGACTTTTG TAATACATTC 
AAGCTCTACC GGGGCAAGAC ACAGGAGGAG ACTGAAGACC CATCAGTCAT TGGGGAGTTT 
AAGGGACTCT TCAAAATTTA TCCTCTCCCA GAAGACCCAG CCATCCCCTT GCCCCCGAGG 
CAGTTCCACC AGCTGGCTTC CCAGGGGCCC CAGGAATGCC TGGTCCGCGT CTACATTATC 
CGGGCGTTTG GCCTGCAGCC CAAGGATCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCGGT GAGTGACCAG GACAGCTACA TCCCTTGTAC TCTGGAGCCT 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC 
ACCCTCTATG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGGGAGAC GGTCATTGAC 
CTGGAGAACA GGCTGCTGTC TAAGTTCGGG GCCCGCTGTG GACTCCCCCA GACCTACTGC 
GTCTCTGGAC CCAACCAGTG GAGGGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTGTTC 
TGCCAGCAGC ACCGAGTCAA GGCCCCCGTG TACCGGACGG ACCGCGTGGT GTTTCAGGAT 
AAAGAATACA CCGTCGAAGA AATAGAGGCT GGCAGGGTCC CGAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCAGG GGCTGATCCC TGAGCACGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCGGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGGCCGGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACCAAGGA TGTGATCCTG 
GATGACCTCA GCATCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT 
GGCTTTGAAG AACACAAGCA AAAGACAGAT GTACATTATC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCGT CTTCCCTTTT GACTACCTGC CAGCTGAGCA GGTCTGCACC 
GTCTCCAAGA AGGATGCATT CTGGAGGCTG GACAAGACGG AGAGCAAGAT CCCTGCACGA 
GTGGTATTCC AGATCTGGGA CAACGACAAG TTCTCCTTCG ATGACTTTCT GGGCTCTCTG 
CAGCTGGACC TTAACCACAT GCCCAAGCCC GCCAAGACAG CTGAGAAGTG CTCTGCGGAC 
CAGCTGGAAG ACACCTTCCA CCCAGAGCGG TTTGTGTCTC TTTTTGAACA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAGATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCAGC CGGCCACGGC 
CGGGATGAAC CTAACATGAA CCCCAAGCTC GAGGACCCAA GGCGGCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGCTGT 
GCCATCATCT TCTTCCTCAT TCTCTTCATC TTCCTGCTCT TCTTGGGCAT CTTTGTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808149.1
CDS	130..6537
Translation	MLSCQLVRASNLPSLKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGIPLDQGSELLVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPTFPPLTPLEPSPTLPDMDTVAGGGQSLAETWSLLSDSTVDTRHSGRKWLAPTDTGGEEDTEDQGLTGDEAEPFLDQSGALGPGAPSTPKKQPSHPPPYHPGGGRKRSAPAPSKPLSDKPQDFQIRVQVIKGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPVFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRLDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIESNLLRPIGVALRGAHFCLKVFRAEDLPQMDDAVMDNVRQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVVDWDRLTHNDIVATTYLSMSKISASGGEIEEEPAGVVKPPPATELDDHLGFLPTFGPCYINLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVERSEQKVEALSADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAVQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDIRHRVEAQNQLLRIADQLEAGLEQVHLALKAQCSDEDADSLVAQLMDELIAGCSQPLGDVQEMPSATHLDQYLYQLRTRHLSQITEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGTSYCGKNCGKLQTIFLKYPMEGVPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDITGRIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAVVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPSSIAEQPPSIVVELYDHDTYGVDEFMGRCICQPSLERTPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTTGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQSVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVVKVIDNRQFGRRPVVGQCTIRSLEGFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQFADGLSGLAPTNMASSPSSLHEEEFIDWWSKFFASIGESEKCGSYLEKDFDTLKVYDTSLENVKAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPLPPRQFHQLASQGPQECLVRVYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDSYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTVEEIEAGRVPNPHLGPVEERLALHVLQQQGLIPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSADQLEDTFHPERFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGHGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIIFFLILFIFLLFLGIFVYSFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_010809847.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809847.2

ORF Insert Sequence:

ATGCTGAGCT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTCTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGTGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAATGTGA ACCCTGTGTG GAATGAGGGA TTCGAGTGGG ACCTCAAGGG TATCCCCCTG 
GACCAGGGCT CCGAGCTTCT TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACAT CCCCCTCCGG GAAGTCCTCG CCACCCCCAG CCTGTCTGCC 
AGCTTCAATG CCCCACTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCGCT GCCTGGAGCC GTGCCCACCT TCCCACCCTT AACACCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ACGGTGGCTG GTGGGGGCCA GAGCCTGGCC 
GAGACCTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GACACTCTGG AAGGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACGGGAGAC 
GAGGCCGAGC CATTCCTGGA CCAGAGCGGG GCTCTGGGCC CGGGGGCCCC CAGCACCCCG 
AAGAAACAAC CTTCCCATCC GCCCCCCTAC CACCCCGGAG GGGGAAGGAA GAGAAGCGCG 
CCTGCGCCCA GCAAGCCTCT GTCGGACAAG CCGCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCAAGGGAC GCCAGCTGCC AGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCCGCT 
GGGCAGACCA AGCGGACGCG GATCCACAAA GGAAACAGCC CCGTCTTCAA TGAGACTCTC 
TTCTTCAACG TGTTTGACTC TCCCGCGGAG CTGTTCAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG AACAGATGCT CTCATCGGGG AGTTCCGGCT GGACGTGGGC 
AGCATCTACC GAGAGCCCCG ACATGCCTAC CTCAGGAAGT GGCTGCTGCT CTCAGATCCC 
GACGATTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCGA GTCTCTGTGT GCTGGGGCCT 
GGAGATGAAG CCCCCCTGGA GAGAAAAGAC CCCTCTGAAG ACAAGGAGGA CATCGAAAGC 
AACCTGCTTA GGCCGATCGG CGTGGCCCTC CGTGGAGCGC ACTTCTGTCT GAAGGTCTTC 
AGGGCCGAGG ACTTGCCACA GATGGACGAT GCCGTGATGG ACAACGTGAG GCAGATCTTC 
GGCTTTGACA GTAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCAGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACGGCCAATC CTCAGTGGAA CCAGAGCATC 
ACACTGCCTG CCATGTTTCC ATCCATGTGT GAGAAAATGA GGATTCGTGT CGTAGACTGG 
GACCGCCTCA CCCACAACGA TATTGTGGCC ACCACCTACC TGAGTATGTC GAAGATCTCT 
GCCTCTGGAG GCGAGATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTCC TCCAGCCACA 
GAACTGGACG ACCATCTAGG CTTCCTCCCC ACCTTCGGGC CCTGCTACAT CAACCTCTAT 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GATCCCTATG CAGAGCTCAA CACGGGCAAG 
GGAGAAGGCG TGGCCTACCG AGGCCGGCTC CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CGCAGTGAAC AGAAAGTGGA GGCTCTCTCT GCCGATGACA TCCTTCGGGT AGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTGCAGGAT 
GTCGATGACG CCGTCCAGTT CGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACC 
ACCTGCCTGC CGCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCGTC TTACTGGGAG 
GACATCCGCC ACAGAGTTGA GGCTCAGAAC CAGCTTCTCA GGATTGCCGA CCAGCTGGAA 
GCCGGCCTGG AGCAAGTCCA CTTGGCCCTG AAGGCTCAGT GCTCCGACGA GGACGCGGAC 
TCCCTGGTGG CTCAGCTGAT GGATGAGCTC ATCGCAGGCT GCAGCCAACC CCTGGGTGAT 
GTCCAGGAGA TGCCCTCTGC CACCCACCTG GACCAGTACC TGTACCAGCT GCGCACCCGC 
CACCTGAGCC AGATCACCGA GGCGGCCCAG GCCCTGAAGC TCGGCCACAG TGAGCTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGGCTCC GTGCCCTGGC TGACGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTTCAGG GGGACAAGCG GGTGGCGTAC 
CAGCGGGTGC CTGCCCATGA GGTCCTCTTC TCCCGCCGGG GCACCAGCTA CTGTGGCAAG 
AACTGTGGAA AACTGCAGAC GATATTTCTG AAATATCCGA TGGAAGGGGT GCCCAGAGCC 
CGGATGCCGG TGCAGATTCG GGTCAGGCTC TGGTTTGGAC TCTCTGTGGA TGAAAAGGAG 
TTCAACCAGT TTGCTGAGGG CAAGCTCTCC GTCTTCGCTG AAACTTATGA GAACCAGACC 
AAGCTGGCCC TGGTTGGGAA CTGGGGCACA ACTGGCCTCA CCTACCCCAA GTTTTCTGAC 
ATCACGGGCA GGATCAAGCT ACCCAAGGAC AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCTGGAGACT GGTTCGTGTG TCCAGAGAAG ACACTGCTTC ACGACACTGA TGCTGGCCAC 
CTGAGCTTCG TGGAGGAGGT ATTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAGTGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT GAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCGC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CCGAGAAGAT GTACTACACA CACCGGCGGC GGCGCTGGGT CCGTCTGCGC 
CGGAGGGACC TCAGCCAGAT GGAGGCGTTG AAGCGGCACA GGCAGGCGGA GGCAGAGGGC 
GAGGGCTGGG AGTACGCCTC GCTCTTCGGC TGGAAGTTCC ATCTGGAGTA CCGCAAGACA 
GACGCCTTTC GTCGTCGCCG CTGGCGCCGC CGCATGGAGC CCTTGGAGAA GACAGGGCCT 
GCGGCTGTGT TTGCCCTTGA GGGGGCCCTG GGTGGCGTGG TGGACGACAG GAGTGAAGAT 
TCCGTGTCCG TCTCCACCTT GAGTTTTGGT GTGAACAGAC CCACGATTTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTACGCTGC TACATGTACC AGGCCCGGGA TCTGCCTGCA 
ATGGACAAGG ACTCTTTTTC CGATCCCTAT GCAGTCGTCT CCTTCCTGCA CCAAAGCCAG 
AAGACGGTGG TGGCTAAGAA CACCCTAAAC CCCACCTGGG ACCAGACGCT CATCTTCTAC 
GAGATCGAGA TCTTTGGCGA GCCAAGCAGC ATCGCCGAGC AGCCTCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGTGTA GATGAGTTCA TGGGACGTTG CATCTGTCAG 
CCGAGTCTGG AACGGACGCC TCGGCTGGCC TGGTTCCCGC TGACGAGGGG CAGCCAGCCC 
GCCGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCTGC CATCCACCAT 
ATTCCTGGTT TTGAGGTACA GGACACAACA GGGATCCTGG AAGAGTCAGA GGACACGGAC 
CTGCCCTACC CACCGCCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACCGC CATCGAGATC CTGGCGTGGG GTCTTCGGAA CATGAAGAGC 
TACCAGCTGG CCAGCGTGTC CTCCCCGAGC CTGGTGGTGG AGTGCGGGGG CCAGTCTGTA 
CAATCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTG TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCCGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
GGCTTCCTGT GTGATCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGTCCTGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATCC AGTTTGCAGA TGGTCTCTCG GGCTTGGCCC CCACTAACAT GGCATCTTCT 
CCATCCAGTC TTCATGAGGA GGAGTTCATT GATTGGTGGA GCAAATTCTT TGCTTCAATT 
GGGGAGAGCG AAAAGTGCGG GTCCTACCTA GAGAAGGACT TTGACACTCT AAAGGTCTAT 
GACACATCCC TGGAGAATGT GAAGGCCTTT GAGGGCCTGT CTGACTTTTG TAATACATTC 
AAGCTCTACC GGGGCAAGAC ACAGGAGGAG ACTGAAGACC CATCAGTCAT TGGGGAGTTT 
AAGGGACTCT TCAAAATTTA TCCTCTCCCA GAAGACCCAG CCATCCCCTT GCCCCCGAGG 
CAGTTCCACC AGCTGGCTTC CCAGGGGCCC CAGGAATGCC TGGTCCGCGT CTACATTATC 
CGGGCGTTTG GCCTGCAGCC CAAGGATCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCGGT GAGTGACCAG GACAGCTACA TCCCTTGTAC TCTGGAGCCT 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC 
ACCCTCTATG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGGGAGAC GGTCATTGAC 
CTGGAGAACA GGCTGCTGTC TAAGTTCGGG GCCCGCTGTG GACTCCCCCA GACCTACTGC 
GTCTCTGGAC CCAACCAGTG GAGGGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTGTTC 
TGCCAGCAGC ACCGAGTCAA GGCCCCCGTG TACCGGACGG ACCGCGTGGT GTTTCAGGAT 
AAAGAATACA CCGTCGAAGA AATAGAGGCT GGCAGGGTCC CGAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCCTT GCACGTCCTC CAGCAGCAGG GGCTGATCCC TGAGCACGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCGGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGGCCGGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACCAAGGA TGTGATCCTG 
GATGACCTCA GCATCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGGTT 
GGCTTTGAAG AACACAAGCA AAAGACAGAT GTACATTATC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCGT CTTCCCTTTT GACTACCTGC CAGCTGAGCA GGTCTGCACC 
GTCTCCAAGA AGGATGCATT CTGGAGGCTG GACAAGACGG AGAGCAAGAT CCCTGCACGA 
GTGGTATTCC AGATCTGGGA CAACGACAAG TTCTCCTTCG ATGACTTTCT GGGCTCTCTG 
CAGCTGGACC TTAACCACAT GCCCAAGCCC GCCAAGACAG CTGAGAAGTG CTCTGCGGAC 
CAGCTGGAAG ACACCTTCCA CCCAGAGCGG TTTGTGTCTC TTTTTGAACA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT GGCGGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAGATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCAGC CGGCCACGGC 
CGGGATGAAC CTAACATGAA CCCCAAGCTC GAGGACCCAA GGCGGCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCC TGTGGCGGCG GTTCCGCTGT 
GCCATCATCT TCTTCCTCAT TCTCTTCATC TTCCTGCTCT TCTTGGGCAT CTTTGTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DYSF
Entrez Gene ID	508157
Full Name	dysferlin
Synonyms	FER1L1
General protein information	Preferred Names dysferlin Names dysferlin Dystrophy-associated fer-1-like protein Fer-1-like protein 1 dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	DYSF	NP_001124454.1
	DYSF	XP_002799336.1
Caenorhabditis elegans (roundworm)	fer-1	NP_492337.1
Caenorhabditis elegans (roundworm)	ferl-1	NP_491602.2
Rattus norvegicus (Norway rat)	Dysf	NP_001101339.1
Danio rerio (zebrafish)	dysf	NP_001244122.1
Mus musculus (house mouse)	Dysf	NP_067444.2
Canis lupus familiaris (dog)	DYSF	XP_003432278.1
Bos taurus (cattle)	DYSF	NP_001095960.1
Pan troglodytes (chimpanzee)	DYSF	XP_003309136.1
Gallus gallus (chicken)	DYSF	XP_423126.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DYSF cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: