Dysf cDNA ORF clone, Mus musculus(house mouse)

The following Dysf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dysf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu81738 XM_017321594.1
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu37313 XM_006506185.3
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu37311 XM_006506183.4
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu37310 XM_006506182.3
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu37312 XM_006506184.3
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu37292 XM_006506164.4
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu37299 XM_006506171.4
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu37301 XM_006506173.4
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu37303 XM_006506175.4
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu37305 XM_006506177.4
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu37307 XM_006506179.4
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu37309 XM_006506181.4
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu89210 XM_030255407.1
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu16347 NM_001077694.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1546.30
$2209.00
OMu16405 NM_021469.3
Latest version!
Mus musculus dysferlin (Dysf), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37308 NM_001310152.1
Latest version!
Mus musculus dysferlin (Dysf), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37291 XM_006506163.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37293 XM_006506165.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37294 XM_006506166.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37295 XM_006506167.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37296 XM_006506168.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37297 XM_006506169.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37298 XM_006506170.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37300 XM_006506172.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37302 XM_006506174.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37304 XM_006506176.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37306 XM_006506178.2
Latest version!
Mus musculus dysferlin (Dysf), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu37309 XM_006506181.3 Mus musculus dysferlin (Dysf), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37305 XM_006506177.3 Mus musculus dysferlin (Dysf), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37301 XM_006506173.3 Mus musculus dysferlin (Dysf), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37292 XM_006506164.3 Mus musculus dysferlin (Dysf), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37299 XM_006506171.3 Mus musculus dysferlin (Dysf), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37303 XM_006506175.3 Mus musculus dysferlin (Dysf), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37307 XM_006506179.3 Mus musculus dysferlin (Dysf), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu37311 XM_006506183.3 Mus musculus dysferlin (Dysf), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu81738
    Clone ID Related Accession (Same CDS sequence) XM_017321594.1
    Accession Version XM_017321594.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCCAGC CCCTGTGTGA CATCCACGAG ATACCTTCTG CCACCCACCT TGATCAGTAC 
    CTGCTCCGGC TACGCACCCG TCACCTGAGC CAGATCAAAG AAGCTGCTCT GGCCTTGAAG
    CTTGGCCATA GTGAGCTCTC CACTGCCCTG GAGCAGGCCG AGGACTGGCT TCTGCATCTC
    CGTGCCCTGG CCGAGGAGCC CCAGAACAGC CTGCCGGACA TCATCATCTG GATGCTGCAG
    GGTGACAAGC GTGTGGCCTA CCAGCGGGTG CCTGCCCATG AAGTCCTCTT CTCCCGGCGG
    GGCCCCAGCT ACTGCGGCAG GAACTGTGGG AAGCTGCAGA CCATCTTTCT GAAATATCCT
    ATGGAAGGTA TGCCTGGGGC CCGAATGCCC GTGCAGATTC GGATCAAGCT CTGGTTCGGG
    CTCTCTGTGG ATGAGAAGGA ATTCAATCAG TTTGCTGAGG GAAAGCTCTC TGTCTTTGCC
    GAAACGTATG AGAACCAGAC AAAGCTGGCA CTGGTCGGGA ACTGGGGCAC AACGGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCACAGGC AAGATCAAAC TACCAAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGGCCTG GGCAGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGATGCTG ACGCTGGTCA TCTGAGCTTC GTGGAGGAGG TGTTCGAGAA CCAGACCCGG
    CTCCCTGGGG GCCAGTGGAT TTACATGAGT GATAACTACA CAGATGTGAA TGGGGAGAAG
    GTACTTCCGA AGGATGATAT TGAATGCCCG CTGGGCTGGA AGTGGGAAGA TGAAGAATGG
    TCCACAGACC TCAATCGGGC TGTTGATGAA CAAGGCTGGG AGTACAGCAT CACCATACCC
    CCAGACCGAA AGCCAAAACA CTGGGTCCCT GTTGAGAAGA TGTATTACAC TCACAGGCGT
    CGACGCTGGG TCCGACTGCG CAGGAGGGAC CTCAGCCAGA TGGAAGCACT GAAGAGGCAC
    AGGCAGGCAG AGGCCGAGGG CGAGGGCTGG GAGTATGCCT CTCTCTTCGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG
    CCGCTGGAGA AGACGGGACC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCATG
    GTGGATGACA AGAGCGAAGA CTCCATGTCT GTTTCTACCC TGAGCTTTGG CGTTAACAGA
    CCCACAATCT CCTGCATCTT TGACTATGGG AACCGCTACC ATCTGCGCTG CTACCTGTAC
    CAGGCCCGAG ACCTGCCAGC CATGGACAAG GACTCCTTCT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ATCAAAGCCA GAAGACAGTG GTGGAGAAGA ACACCCTGAA TCCCACCTGG
    GATCAAACCC TCATCTTCTA TGAGATTGAG ATCTTCGGCG AGCCAGCCAG TATCGCCGAG
    CATCCGCCCT GCATCGTGGT GGAGCTCTAT GACCACGACA CCTATGGTGC TGACGAGTTC
    ATGGGTCGCT GTATTTGTCA GCCAAGCCTG GAACGGATGC CCCGCCTGGC CTGGTTCCCA
    CTGACCCGGG GCAGCCAGCC TGCCGGCGAG CTGCTGGCAG CGTTCGAGCT TATCCAGAGA
    GAGAAGCCAG CCATCCATCA CATCCCTGGC TTTGAGATGC ATGAAACTTC AAGGATTTTG
    GATGAGCTAT GCACGCCTGC CTTCTTCCTG CAGGGCTTCC TTCGGACGAG CACGACGGAG
    GACACAGATT TGCCTTACCC ACCACCTCAA AGGGAGGCCA ACATCTACAT GGTTCCCCAG
    AACATTAAGC CAGCTCTCCA ACGTACTGCC ATTGAGATCC TGGCCTGGGG CCTGCGGAAC
    ATGAAGAGCT ATCAGATGGC CAGCATCTCT TCCCCCAGCC TCGTGGTGGA GTGTGGTGGG
    CAGACAGTGC AGTCCTGTGT CATCAGAAAC CTTCGGAAGA ACCCCAACTT TGATGTCTGT
    ACTCTCTTCA TGGAAGTGAT GTTACCCAGA GAGGACCTCT ACTGTCCACC TATTGTGGTC
    AAGGTCATTG ATAATCGCCA GTTTGGCCGC CGGCCAGTTG TGGGTCAGTG TACCATTCGC
    TCCCTGGAGA ACTTCCTGTG CGACCCATAC TCAGCAGAGA GTCCATCCCC ACAGGGTGGC
    CCAGATGATG TGAGCTTACT CAGCCCCGGG GAAGATGTGC TGATTGACAT CGATGACAAG
    GAGCCTCTCA TTCCTGTCCA GCTTACAGAT GGGCTGTCAA GCTTGGGCCC CACTAACCTG
    ACGCCTTCTC CATCCAGTCC TCATAAGATC CTCCTGGAGG AGGAGTTCAT TGACTGGTGG
    AGCAAATTCT TTGCTTCTGT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT
    TTTGACACCT TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCTTT TGGAGGCCTG
    TCCGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAGGA CTCAGGAGGA GACAGATGAT
    CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA
    GCCATCCCCA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC
    TTGGTCCGTA TTTATATCGT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG
    TGTGACCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATCCCTTGCA CCCTGGAGCC TGTATTCGGA AAGATGTTTG AACTCACCTG CACTCTACCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAC GATTATGACC TGCTCTCCAA GGATGAGAAG
    ATTGGGGAGA CGGTCATAGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT
    GGGCTCCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA GCTCCGCCCC
    TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGATCA AGGCCCCCGT GTACCGGACA
    GACCGAGTGA CGTTTCAGGA TAAGGACTAC ACCATTGAGG AGATAGAGGC TGGCAGACTC
    CCAAACCCAC ACCTGGGCCC AGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAGCAACAA
    GGCTTGGTTC CTGAGCATGT GGAGTCACGG CCTCTTTATA GTCCTCTGCA GCCAGATATC
    GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA
    CCTCCCTTCA ACATCACCCC ACGGAAAGCT AGAAGGTTTT TTCTGCGTTG TATTATTTGG
    AACACAAAAG ATGTGATCTT GGATGACCTC AGTCTCACGG GGGAGAAGAT GAGTGACATC
    TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAGC AGAAGACAGA TGTGCACTAC
    CGCTCCCTGG GTGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG
    CCTGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCCT TCTGGAGACT CGACAAGACA
    GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATCTGGG ACAATGACAA ATTCTCTTTT
    GATGACTTTC TGGGCTCTCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC
    CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGAGAAG
    AAGATGTTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TTGCAGAAAG TGAACATGAA
    GAGCGGCCTG CTGGCCAAGG TCGGGATGAA CCCAACATGA ATCCGAAGCT AGAGGATCCA
    AGGCGCCCCG ATACTTCTTT CCTGTGGTTC ACCTCCCCGT ACAAGACCAT GAAGTTCATC
    TTGTGGCGAC GCTTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC
    TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177083.1
    CDS138..4346
    Translation

    Target ORF information:

    RefSeq Version XM_017321594.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCCAGC CCCTGTGTGA CATCCACGAG ATACCTTCTG CCACCCACCT TGATCAGTAC 
    CTGCTCCGGC TACGCACCCG TCACCTGAGC CAGATCAAAG AAGCTGCTCT GGCCTTGAAG
    CTTGGCCATA GTGAGCTCTC CACTGCCCTG GAGCAGGCCG AGGACTGGCT TCTGCATCTC
    CGTGCCCTGG CCGAGGAGCC CCAGAACAGC CTGCCGGACA TCATCATCTG GATGCTGCAG
    GGTGACAAGC GTGTGGCCTA CCAGCGGGTG CCTGCCCATG AAGTCCTCTT CTCCCGGCGG
    GGCCCCAGCT ACTGCGGCAG GAACTGTGGG AAGCTGCAGA CCATCTTTCT GAAATATCCT
    ATGGAAGGTA TGCCTGGGGC CCGAATGCCC GTGCAGATTC GGATCAAGCT CTGGTTCGGG
    CTCTCTGTGG ATGAGAAGGA ATTCAATCAG TTTGCTGAGG GAAAGCTCTC TGTCTTTGCC
    GAAACGTATG AGAACCAGAC AAAGCTGGCA CTGGTCGGGA ACTGGGGCAC AACGGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCACAGGC AAGATCAAAC TACCAAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGGCCTG GGCAGGAGAT TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGATGCTG ACGCTGGTCA TCTGAGCTTC GTGGAGGAGG TGTTCGAGAA CCAGACCCGG
    CTCCCTGGGG GCCAGTGGAT TTACATGAGT GATAACTACA CAGATGTGAA TGGGGAGAAG
    GTACTTCCGA AGGATGATAT TGAATGCCCG CTGGGCTGGA AGTGGGAAGA TGAAGAATGG
    TCCACAGACC TCAATCGGGC TGTTGATGAA CAAGGCTGGG AGTACAGCAT CACCATACCC
    CCAGACCGAA AGCCAAAACA CTGGGTCCCT GTTGAGAAGA TGTATTACAC TCACAGGCGT
    CGACGCTGGG TCCGACTGCG CAGGAGGGAC CTCAGCCAGA TGGAAGCACT GAAGAGGCAC
    AGGCAGGCAG AGGCCGAGGG CGAGGGCTGG GAGTATGCCT CTCTCTTCGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG
    CCGCTGGAGA AGACGGGACC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCATG
    GTGGATGACA AGAGCGAAGA CTCCATGTCT GTTTCTACCC TGAGCTTTGG CGTTAACAGA
    CCCACAATCT CCTGCATCTT TGACTATGGG AACCGCTACC ATCTGCGCTG CTACCTGTAC
    CAGGCCCGAG ACCTGCCAGC CATGGACAAG GACTCCTTCT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ATCAAAGCCA GAAGACAGTG GTGGAGAAGA ACACCCTGAA TCCCACCTGG
    GATCAAACCC TCATCTTCTA TGAGATTGAG ATCTTCGGCG AGCCAGCCAG TATCGCCGAG
    CATCCGCCCT GCATCGTGGT GGAGCTCTAT GACCACGACA CCTATGGTGC TGACGAGTTC
    ATGGGTCGCT GTATTTGTCA GCCAAGCCTG GAACGGATGC CCCGCCTGGC CTGGTTCCCA
    CTGACCCGGG GCAGCCAGCC TGCCGGCGAG CTGCTGGCAG CGTTCGAGCT TATCCAGAGA
    GAGAAGCCAG CCATCCATCA CATCCCTGGC TTTGAGATGC ATGAAACTTC AAGGATTTTG
    GATGAGCTAT GCACGCCTGC CTTCTTCCTG CAGGGCTTCC TTCGGACGAG CACGACGGAG
    GACACAGATT TGCCTTACCC ACCACCTCAA AGGGAGGCCA ACATCTACAT GGTTCCCCAG
    AACATTAAGC CAGCTCTCCA ACGTACTGCC ATTGAGATCC TGGCCTGGGG CCTGCGGAAC
    ATGAAGAGCT ATCAGATGGC CAGCATCTCT TCCCCCAGCC TCGTGGTGGA GTGTGGTGGG
    CAGACAGTGC AGTCCTGTGT CATCAGAAAC CTTCGGAAGA ACCCCAACTT TGATGTCTGT
    ACTCTCTTCA TGGAAGTGAT GTTACCCAGA GAGGACCTCT ACTGTCCACC TATTGTGGTC
    AAGGTCATTG ATAATCGCCA GTTTGGCCGC CGGCCAGTTG TGGGTCAGTG TACCATTCGC
    TCCCTGGAGA ACTTCCTGTG CGACCCATAC TCAGCAGAGA GTCCATCCCC ACAGGGTGGC
    CCAGATGATG TGAGCTTACT CAGCCCCGGG GAAGATGTGC TGATTGACAT CGATGACAAG
    GAGCCTCTCA TTCCTGTCCA GCTTACAGAT GGGCTGTCAA GCTTGGGCCC CACTAACCTG
    ACGCCTTCTC CATCCAGTCC TCATAAGATC CTCCTGGAGG AGGAGTTCAT TGACTGGTGG
    AGCAAATTCT TTGCTTCTGT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT
    TTTGACACCT TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCTTT TGGAGGCCTG
    TCCGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAGGA CTCAGGAGGA GACAGATGAT
    CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA
    GCCATCCCCA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC
    TTGGTCCGTA TTTATATCGT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG
    TGTGACCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATCCCTTGCA CCCTGGAGCC TGTATTCGGA AAGATGTTTG AACTCACCTG CACTCTACCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAC GATTATGACC TGCTCTCCAA GGATGAGAAG
    ATTGGGGAGA CGGTCATAGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT
    GGGCTCCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA GCTCCGCCCC
    TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGATCA AGGCCCCCGT GTACCGGACA
    GACCGAGTGA CGTTTCAGGA TAAGGACTAC ACCATTGAGG AGATAGAGGC TGGCAGACTC
    CCAAACCCAC ACCTGGGCCC AGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAGCAACAA
    GGCTTGGTTC CTGAGCATGT GGAGTCACGG CCTCTTTATA GTCCTCTGCA GCCAGATATC
    GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA
    CCTCCCTTCA ACATCACCCC ACGGAAAGCT AGAAGGTTTT TTCTGCGTTG TATTATTTGG
    AACACAAAAG ATGTGATCTT GGATGACCTC AGTCTCACGG GGGAGAAGAT GAGTGACATC
    TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAGC AGAAGACAGA TGTGCACTAC
    CGCTCCCTGG GTGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG
    CCTGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCCT TCTGGAGACT CGACAAGACA
    GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATCTGGG ACAATGACAA ATTCTCTTTT
    GATGACTTTC TGGGCTCTCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC
    CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGAGAAG
    AAGATGTTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TTGCAGAAAG TGAACATGAA
    GAGCGGCCTG CTGGCCAAGG TCGGGATGAA CCCAACATGA ATCCGAAGCT AGAGGATCCA
    AGGCGCCCCG ATACTTCTTT CCTGTGGTTC ACCTCCCCGT ACAAGACCAT GAAGTTCATC
    TTGTGGCGAC GCTTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC
    TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37313
    Clone ID Related Accession (Same CDS sequence) XM_006506185.3
    Accession Version XM_006506185.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTCTCCAG CCCTTAGCAA CCCTATTCAC CACATGACAT CTCTGTTTCC TCCTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506248.1
    CDS282..3881
    Translation

    Target ORF information:

    RefSeq Version XM_006506185.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506185.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTCTCCAG CCCTTAGCAA CCCTATTCAC CACATGACAT CTCTGTTTCC TCCTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37311
    Clone ID Related Accession (Same CDS sequence) XM_006506183.3 , XM_006506183.4
    Accession Version XM_006506183.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506183.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATGTCCCCC ACTACTGTGT GTGCTCATCT CCCCAGCCTT GGGGACAGAA GCCAAACTTC
    CTTTGGATCT GGATGTGCCA GCTGCCCTCT GTTCACTTAA AACCACAGCC AGCATGCACT
    ATCTATGAAT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506246.1
    CDS267..5021
    Translation

    Target ORF information:

    RefSeq Version XM_006506183.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506183.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATGTCCCCC ACTACTGTGT GTGCTCATCT CCCCAGCCTT GGGGACAGAA GCCAAACTTC
    CTTTGGATCT GGATGTGCCA GCTGCCCTCT GTTCACTTAA AACCACAGCC AGCATGCACT
    ATCTATGAAT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37310
    Clone ID Related Accession (Same CDS sequence) XM_006506182.3
    Accession Version XM_006506182.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATGGCTGCA AGAGTGCCAG AAATAAGGAA AGAAGATCCT CCTGGAGGAG GAGTTCATTG
    ACTGGTGGAG CAAATTCTTT GCTTCTGTCG GGGAGAGGGA AAAGTGTGGT TCCTACCTGG
    AGAAGGATTT TGACACCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506245.1
    CDS267..5027
    Translation

    Target ORF information:

    RefSeq Version XM_006506182.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506182.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATGGCTGCA AGAGTGCCAG AAATAAGGAA AGAAGATCCT CCTGGAGGAG GAGTTCATTG
    ACTGGTGGAG CAAATTCTTT GCTTCTGTCG GGGAGAGGGA AAAGTGTGGT TCCTACCTGG
    AGAAGGATTT TGACACCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37311
    Clone ID Related Accession (Same CDS sequence) XM_006506183.3 , XM_006506183.4
    Accession Version XM_006506183.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506183.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATGTCCCCC ACTACTGTGT GTGCTCATCT CCCCAGCCTT GGGGACAGAA GCCAAACTTC
    CTTTGGATCT GGATGTGCCA GCTGCCCTCT GTTCACTTAA AACCACAGCC AGCATGCACT
    ATCTATGAAT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506246.1
    CDS267..5021
    Translation

    Target ORF information:

    RefSeq Version XM_006506183.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506183.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATGTCCCCC ACTACTGTGT GTGCTCATCT CCCCAGCCTT GGGGACAGAA GCCAAACTTC
    CTTTGGATCT GGATGTGCCA GCTGCCCTCT GTTCACTTAA AACCACAGCC AGCATGCACT
    ATCTATGAAT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37312
    Clone ID Related Accession (Same CDS sequence) XM_006506184.3
    Accession Version XM_006506184.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    GTCCTCAGAT CTCAGCCCCG AATCCTGTTG CTAACCGGCA TGTTTAATTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506247.1
    CDS265..4878
    Translation

    Target ORF information:

    RefSeq Version XM_006506184.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506184.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    GTCCTCAGAT CTCAGCCCCG AATCCTGTTG CTAACCGGCA TGTTTAATTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37292
    Clone ID Related Accession (Same CDS sequence) XM_006506164.3 , XM_006506164.4
    Accession Version XM_006506164.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506164.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCACA AGCCACTGAC
    CTGGACGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC
    AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCAG AGCTCAACAC AGGAAAGGGA
    GAAGGTGTGG CTTATCGAGG CAGGGTTCTG TTGTCCCTGG AGACCAAACT GGTGGAACAC
    AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT GATGATATCC TGCGTGTGGA GAAGTACCTG
    CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG
    GATGACGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GGAACAAGTT CGACACCACC
    TGCCTGCCCT TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC
    TATTACTTGC CTTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCCTCATA CTGGGAAGAC
    ATCAGCCATC GAATTGAGAT CCAAAACCAG CTCCTCAGGG TCGCTGACCG CCTGGAAGCT
    AACCTGGAGC AGGTCCACTT GGCTCTGAAG GCACAGTGTT CCTCCGAGGA TGTGGACGCC
    CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC
    CACGAGATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGC TCCGGCTACG CACCCGTCAC
    CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC TTGAAGCTTG GCCATAGTGA GCTCTCCACT
    GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCCGA GGAGCCCCAG
    AACAGCCTGC CGGACATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAG
    CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC
    TGTGGGAAGC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGTATGCC TGGGGCCCGA
    ATGCCCGTGC AGATTCGGAT CAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAATTC
    AATCAGTTTG CTGAGGGAAA GCTCTCTGTC TTTGCCGAAA CGTATGAGAA CCAGACAAAG
    CTGGCACTGG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ACAGGCAAGA TCAAACTACC AAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA
    GGAGATTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ATGCTGACGC TGGTCATCTG
    AGCTTCGTGG AGGAGGTGTT CGAGAACCAG ACCCGGCTCC CTGGGGGCCA GTGGATTTAC
    ATGAGTGATA ACTACACAGA TGTGAATGGG GAGAAGGTAC TTCCGAAGGA TGATATTGAA
    TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT
    GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC AAAACACTGG
    GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG
    AGGGACCTCA GCCAGATGGA AGCACTGAAG AGGCACAGGC AGGCAGAGGC CGAGGGCGAG
    GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGACCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGACTCC
    ATGTCTGTTT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC
    TATGGGAACC GCTACCATCT GCGCTGCTAC CTGTACCAGG CCCGAGACCT GCCAGCCATG
    GACAAGGACT CCTTCTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCATCA AAGCCAGAAG
    ACAGTGGTGG AGAAGAACAC CCTGAATCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG
    ATTGAGATCT TCGGCGAGCC AGCCAGTATC GCCGAGCATC CGCCCTGCAT CGTGGTGGAG
    CTCTATGACC ACGACACCTA TGGTGCTGAC GAGTTCATGG GTCGCTGTAT TTGTCAGCCA
    AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCCGGGGCAG CCAGCCTGCC
    GGCGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC
    CCTGGCTTTG AGATGCATGA AACTTCAAGG ATTTTGGATG AGCTATGCAC GCCTGCCTTC
    TTCCTGCAGG GCTTCCTTCG GACGAGCACG ACGGAGGACA CAGATTTGCC TTACCCACCA
    CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TTAAGCCAGC TCTCCAACGT
    ACTGCCATTG AGATCCTGGC CTGGGGCCTG CGGAACATGA AGAGCTATCA GATGGCCAGC
    ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC
    AGAAACCTTC GGAAGAACCC CAACTTTGAT GTCTGTACTC TCTTCATGGA AGTGATGTTA
    CCCAGAGAGG ACCTCTACTG TCCACCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT
    GGCCGCCGGC CAGTTGTGGG TCAGTGTACC ATTCGCTCCC TGGAGAACTT CCTGTGCGAC
    CCATACTCAG CAGAGAGTCC ATCCCCACAG GGTGGCCCAG ATGATGTGAG CTTACTCAGC
    CCCGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CTCTCATTCC TGTCCAGCTT
    ACAGATGGGC TGTCAAGCTT GGGCCCCACT AACCTGACGC CTTCTCCATC CAGTCCTCAT
    AAGATCCTCC TGGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTGTCGGG
    GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCTTGAA GGTCTATGAC
    ACACAGCTGG AGAATGTGGA GGCTTTTGGA GGCCTGTCCG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAGGACTCA GGAGGAGACA GATGATCCAT CTGTCATCGG AGAATTTAAG
    GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCCATGCC CCCGAGACAA
    TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGTATTTA TATCGTCCGA
    GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGCACCCT GGAGCCTGTA
    TTCGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACCT GAAGATCACA
    CTCTACGATT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACGGT CATAGACCTG
    GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGGC TCCCACAGAC CTACTGTGTC
    TCTGGACCAA ATCAGTGGAG AGATCAGCTC CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC
    CAGCAGCACA GGATCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG
    GACTACACCA TTGAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCAGTG
    GAGGAACGCT TAGCCCTGCA TGTCCTTCAG CAACAAGGCT TGGTTCCTGA GCATGTGGAG
    TCACGGCCTC TTTATAGTCC TCTGCAGCCA GATATCGAGC AGGGGAAGCT ACAGATGTGG
    ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG
    AAAGCTAGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT
    GACCTCAGTC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA
    TTCGAAGAAC ACAAGCAGAA GACAGATGTG CACTACCGCT CCCTGGGTGG GGAGGGCAAT
    TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCTG CTGAGCAAGT CTGTGCTGTT
    GCCAAGAAGG ATGCCTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG
    GTCTTCCAGA TCTGGGACAA TGACAAATTC TCTTTTGATG ACTTTCTGGG CTCTCTGCAG
    CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA
    GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA
    ATGACCTTGG AGATTGTTGC AGAAAGTGAA CATGAAGAGC GGCCTGCTGG CCAAGGTCGG
    GATGAACCCA ACATGAATCC GAAGCTAGAG GATCCAAGGC GCCCCGATAC TTCTTTCCTG
    TGGTTCACCT CCCCGTACAA GACCATGAAG TTCATCTTGT GGCGACGCTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506227.1
    CDS555..7019
    Translation

    Target ORF information:

    RefSeq Version XM_006506164.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506164.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCACA AGCCACTGAC
    CTGGACGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC
    AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCAG AGCTCAACAC AGGAAAGGGA
    GAAGGTGTGG CTTATCGAGG CAGGGTTCTG TTGTCCCTGG AGACCAAACT GGTGGAACAC
    AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT GATGATATCC TGCGTGTGGA GAAGTACCTG
    CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG
    GATGACGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GGAACAAGTT CGACACCACC
    TGCCTGCCCT TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC
    TATTACTTGC CTTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCCTCATA CTGGGAAGAC
    ATCAGCCATC GAATTGAGAT CCAAAACCAG CTCCTCAGGG TCGCTGACCG CCTGGAAGCT
    AACCTGGAGC AGGTCCACTT GGCTCTGAAG GCACAGTGTT CCTCCGAGGA TGTGGACGCC
    CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC
    CACGAGATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGC TCCGGCTACG CACCCGTCAC
    CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC TTGAAGCTTG GCCATAGTGA GCTCTCCACT
    GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCCGA GGAGCCCCAG
    AACAGCCTGC CGGACATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAG
    CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC
    TGTGGGAAGC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGTATGCC TGGGGCCCGA
    ATGCCCGTGC AGATTCGGAT CAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAATTC
    AATCAGTTTG CTGAGGGAAA GCTCTCTGTC TTTGCCGAAA CGTATGAGAA CCAGACAAAG
    CTGGCACTGG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ACAGGCAAGA TCAAACTACC AAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA
    GGAGATTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ATGCTGACGC TGGTCATCTG
    AGCTTCGTGG AGGAGGTGTT CGAGAACCAG ACCCGGCTCC CTGGGGGCCA GTGGATTTAC
    ATGAGTGATA ACTACACAGA TGTGAATGGG GAGAAGGTAC TTCCGAAGGA TGATATTGAA
    TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT
    GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC AAAACACTGG
    GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG
    AGGGACCTCA GCCAGATGGA AGCACTGAAG AGGCACAGGC AGGCAGAGGC CGAGGGCGAG
    GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGACCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGACTCC
    ATGTCTGTTT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC
    TATGGGAACC GCTACCATCT GCGCTGCTAC CTGTACCAGG CCCGAGACCT GCCAGCCATG
    GACAAGGACT CCTTCTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCATCA AAGCCAGAAG
    ACAGTGGTGG AGAAGAACAC CCTGAATCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG
    ATTGAGATCT TCGGCGAGCC AGCCAGTATC GCCGAGCATC CGCCCTGCAT CGTGGTGGAG
    CTCTATGACC ACGACACCTA TGGTGCTGAC GAGTTCATGG GTCGCTGTAT TTGTCAGCCA
    AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCCGGGGCAG CCAGCCTGCC
    GGCGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC
    CCTGGCTTTG AGATGCATGA AACTTCAAGG ATTTTGGATG AGCTATGCAC GCCTGCCTTC
    TTCCTGCAGG GCTTCCTTCG GACGAGCACG ACGGAGGACA CAGATTTGCC TTACCCACCA
    CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TTAAGCCAGC TCTCCAACGT
    ACTGCCATTG AGATCCTGGC CTGGGGCCTG CGGAACATGA AGAGCTATCA GATGGCCAGC
    ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC
    AGAAACCTTC GGAAGAACCC CAACTTTGAT GTCTGTACTC TCTTCATGGA AGTGATGTTA
    CCCAGAGAGG ACCTCTACTG TCCACCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT
    GGCCGCCGGC CAGTTGTGGG TCAGTGTACC ATTCGCTCCC TGGAGAACTT CCTGTGCGAC
    CCATACTCAG CAGAGAGTCC ATCCCCACAG GGTGGCCCAG ATGATGTGAG CTTACTCAGC
    CCCGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CTCTCATTCC TGTCCAGCTT
    ACAGATGGGC TGTCAAGCTT GGGCCCCACT AACCTGACGC CTTCTCCATC CAGTCCTCAT
    AAGATCCTCC TGGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTGTCGGG
    GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCTTGAA GGTCTATGAC
    ACACAGCTGG AGAATGTGGA GGCTTTTGGA GGCCTGTCCG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAGGACTCA GGAGGAGACA GATGATCCAT CTGTCATCGG AGAATTTAAG
    GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCCATGCC CCCGAGACAA
    TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGTATTTA TATCGTCCGA
    GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGCACCCT GGAGCCTGTA
    TTCGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACCT GAAGATCACA
    CTCTACGATT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACGGT CATAGACCTG
    GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGGC TCCCACAGAC CTACTGTGTC
    TCTGGACCAA ATCAGTGGAG AGATCAGCTC CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC
    CAGCAGCACA GGATCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG
    GACTACACCA TTGAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCAGTG
    GAGGAACGCT TAGCCCTGCA TGTCCTTCAG CAACAAGGCT TGGTTCCTGA GCATGTGGAG
    TCACGGCCTC TTTATAGTCC TCTGCAGCCA GATATCGAGC AGGGGAAGCT ACAGATGTGG
    ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG
    AAAGCTAGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT
    GACCTCAGTC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA
    TTCGAAGAAC ACAAGCAGAA GACAGATGTG CACTACCGCT CCCTGGGTGG GGAGGGCAAT
    TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCTG CTGAGCAAGT CTGTGCTGTT
    GCCAAGAAGG ATGCCTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG
    GTCTTCCAGA TCTGGGACAA TGACAAATTC TCTTTTGATG ACTTTCTGGG CTCTCTGCAG
    CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA
    GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA
    ATGACCTTGG AGATTGTTGC AGAAAGTGAA CATGAAGAGC GGCCTGCTGG CCAAGGTCGG
    GATGAACCCA ACATGAATCC GAAGCTAGAG GATCCAAGGC GCCCCGATAC TTCTTTCCTG
    TGGTTCACCT CCCCGTACAA GACCATGAAG TTCATCTTGT GGCGACGCTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37299
    Clone ID Related Accession (Same CDS sequence) XM_006506171.3 , XM_006506171.4
    Accession Version XM_006506171.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506171.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGT GGACGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC
    TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCAGAG
    CTCAACACAG GAAAGGGAGA AGGTGTGGCT TATCGAGGCA GGGTTCTGTT GTCCCTGGAG
    ACCAAACTGG TGGAACACAG TGAGCAGAAG GTGGAAGACC TTCCTGCTGA TGATATCCTG
    CGTGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC
    ACAATGCTGC AGGATGTGGA TGACGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGG
    AACAAGTTCG ACACCACCTG CCTGCCCTTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGATGGAT GCCACTACTA TTACTTGCCT TGGGGCAACG TGAAGCCCGT GGTGGTGCTG
    TCCTCATACT GGGAAGACAT CAGCCATCGA ATTGAGATCC AAAACCAGCT CCTCAGGGTC
    GCTGACCGCC TGGAAGCTAA CCTGGAGCAG GTCCACTTGG CTCTGAAGGC ACAGTGTTCC
    TCCGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC
    CAGCCCCTGT GTGACATCCA CGAGATACCT TCTGCCACCC ACCTTGATCA GTACCTGCTC
    CGGCTACGCA CCCGTCACCT GAGCCAGATC AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC
    CATAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC
    CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG GACATCATCA TCTGGATGCT GCAGGGTGAC
    AAGCGTGTGG CCTACCAGCG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC
    AGCTACTGCG GCAGGAACTG TGGGAAGCTG CAGACCATCT TTCTGAAATA TCCTATGGAA
    GGTATGCCTG GGGCCCGAAT GCCCGTGCAG ATTCGGATCA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAATTCAA TCAGTTTGCT GAGGGAAAGC TCTCTGTCTT TGCCGAAACG
    TATGAGAACC AGACAAAGCT GGCACTGGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGATGTCAC AGGCAAGATC AAACTACCAA AGGATAGCTT CCGCCCCTCA
    GCTGGCTGGG CCTGGGCAGG AGATTGGTTC GTGTGTCCAG AGAAGACCCT ACTCCATGAT
    GCTGACGCTG GTCATCTGAG CTTCGTGGAG GAGGTGTTCG AGAACCAGAC CCGGCTCCCT
    GGGGGCCAGT GGATTTACAT GAGTGATAAC TACACAGATG TGAATGGGGA GAAGGTACTT
    CCGAAGGATG ATATTGAATG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC
    CGAAAGCCAA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC
    TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAAG CACTGAAGAG GCACAGGCAG
    GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT GCCTCTCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACAGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG
    GAGAAGACGG GACCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CATGGTGGAT
    GACAAGAGCG AAGACTCCAT GTCTGTTTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA
    ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GCTGCTACCT GTACCAGGCC
    CGAGACCTGC CAGCCATGGA CAAGGACTCC TTCTCTGATC CCTACGCCAT CGTCTCCTTC
    CTGCATCAAA GCCAGAAGAC AGTGGTGGAG AAGAACACCC TGAATCCCAC CTGGGATCAA
    ACCCTCATCT TCTATGAGAT TGAGATCTTC GGCGAGCCAG CCAGTATCGC CGAGCATCCG
    CCCTGCATCG TGGTGGAGCT CTATGACCAC GACACCTATG GTGCTGACGA GTTCATGGGT
    CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC
    CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG
    CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCATGAAA CTTCAAGGAT TTTGGATGAG
    ACGGAGGACA CAGATTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TTAAGCCAGC TCTCCAACGT ACTGCCATTG AGATCCTGGC CTGGGGCCTG
    CGGAACATGA AGAGCTATCA GATGGCCAGC ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT
    GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAT
    GTCTGTACTC TCTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCACCTATT
    GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCCGGC CAGTTGTGGG TCAGTGTACC
    ATTCGCTCCC TGGAGAACTT CCTGTGCGAC CCATACTCAG CAGAGAGTCC ATCCCCACAG
    GGTGGCCCAG ATGATGTGAG CTTACTCAGC CCCGGGGAAG ATGTGCTGAT TGACATCGAT
    GACAAGGAGC CTCTCATTCC TGTCCAGCTT ACAGATGGGC TGTCAAGCTT GGGCCCCACT
    AACCTGACGC CTTCTCCATC CAGTCCTCAT GAGGAGGAGT TCATTGACTG GTGGAGCAAA
    TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC
    ACCTTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CTTTTGGAGG CCTGTCCGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AGGACTCAGG AGGAGACAGA TGATCCATCT
    GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC
    CCCATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC
    CGTATTTATA TCGTCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT
    TGCACCCTGG AGCCTGTATT CGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG
    AAGGACCTGA AGATCACACT CTACGATTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG
    GAGACGGTCA TAGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC
    CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG
    CTCCTCCACC TCTTCTGCCA GCAGCACAGG ATCAAGGCCC CCGTGTACCG GACAGACCGA
    GTGACGTTTC AGGATAAGGA CTACACCATT GAGGAGATAG AGGCTGGCAG ACTCCCAAAC
    CCACACCTGG GCCCAGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAGCA ACAAGGCTTG
    GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT TATAGTCCTC TGCAGCCAGA TATCGAGCAG
    GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC
    TTCAACATCA CCCCACGGAA AGCTAGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA
    AAAGATGTGA TCTTGGATGA CCTCAGTCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG
    AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAGCAGAAGA CAGATGTGCA CTACCGCTCC
    CTGGGTGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCTGCT
    GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCCTTCTGGA GACTCGACAA GACAGAGAGC
    AAGATCCCAG CACGTGTGGT CTTCCAGATC TGGGACAATG ACAAATTCTC TTTTGATGAC
    TTTCTGGGCT CTCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT
    GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGA GAAGAAGATG
    TTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTTGCAG AAAGTGAACA TGAAGAGCGG
    CCTGCTGGCC AAGGTCGGGA TGAACCCAAC ATGAATCCGA AGCTAGAGGA TCCAAGGCGC
    CCCGATACTT CTTTCCTGTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCTTGTGG
    CGACGCTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG
    GGTGTCTTTG TCTACGCCTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTTCGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506234.1
    CDS555..6917
    Translation

    Target ORF information:

    RefSeq Version XM_006506171.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506171.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGT GGACGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC
    TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCAGAG
    CTCAACACAG GAAAGGGAGA AGGTGTGGCT TATCGAGGCA GGGTTCTGTT GTCCCTGGAG
    ACCAAACTGG TGGAACACAG TGAGCAGAAG GTGGAAGACC TTCCTGCTGA TGATATCCTG
    CGTGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC
    ACAATGCTGC AGGATGTGGA TGACGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGG
    AACAAGTTCG ACACCACCTG CCTGCCCTTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGATGGAT GCCACTACTA TTACTTGCCT TGGGGCAACG TGAAGCCCGT GGTGGTGCTG
    TCCTCATACT GGGAAGACAT CAGCCATCGA ATTGAGATCC AAAACCAGCT CCTCAGGGTC
    GCTGACCGCC TGGAAGCTAA CCTGGAGCAG GTCCACTTGG CTCTGAAGGC ACAGTGTTCC
    TCCGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC
    CAGCCCCTGT GTGACATCCA CGAGATACCT TCTGCCACCC ACCTTGATCA GTACCTGCTC
    CGGCTACGCA CCCGTCACCT GAGCCAGATC AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC
    CATAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC
    CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG GACATCATCA TCTGGATGCT GCAGGGTGAC
    AAGCGTGTGG CCTACCAGCG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC
    AGCTACTGCG GCAGGAACTG TGGGAAGCTG CAGACCATCT TTCTGAAATA TCCTATGGAA
    GGTATGCCTG GGGCCCGAAT GCCCGTGCAG ATTCGGATCA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAATTCAA TCAGTTTGCT GAGGGAAAGC TCTCTGTCTT TGCCGAAACG
    TATGAGAACC AGACAAAGCT GGCACTGGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGATGTCAC AGGCAAGATC AAACTACCAA AGGATAGCTT CCGCCCCTCA
    GCTGGCTGGG CCTGGGCAGG AGATTGGTTC GTGTGTCCAG AGAAGACCCT ACTCCATGAT
    GCTGACGCTG GTCATCTGAG CTTCGTGGAG GAGGTGTTCG AGAACCAGAC CCGGCTCCCT
    GGGGGCCAGT GGATTTACAT GAGTGATAAC TACACAGATG TGAATGGGGA GAAGGTACTT
    CCGAAGGATG ATATTGAATG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC
    CGAAAGCCAA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC
    TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAAG CACTGAAGAG GCACAGGCAG
    GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT GCCTCTCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACAGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG
    GAGAAGACGG GACCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CATGGTGGAT
    GACAAGAGCG AAGACTCCAT GTCTGTTTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA
    ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GCTGCTACCT GTACCAGGCC
    CGAGACCTGC CAGCCATGGA CAAGGACTCC TTCTCTGATC CCTACGCCAT CGTCTCCTTC
    CTGCATCAAA GCCAGAAGAC AGTGGTGGAG AAGAACACCC TGAATCCCAC CTGGGATCAA
    ACCCTCATCT TCTATGAGAT TGAGATCTTC GGCGAGCCAG CCAGTATCGC CGAGCATCCG
    CCCTGCATCG TGGTGGAGCT CTATGACCAC GACACCTATG GTGCTGACGA GTTCATGGGT
    CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC
    CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG
    CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCATGAAA CTTCAAGGAT TTTGGATGAG
    ACGGAGGACA CAGATTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TTAAGCCAGC TCTCCAACGT ACTGCCATTG AGATCCTGGC CTGGGGCCTG
    CGGAACATGA AGAGCTATCA GATGGCCAGC ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT
    GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAT
    GTCTGTACTC TCTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCACCTATT
    GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCCGGC CAGTTGTGGG TCAGTGTACC
    ATTCGCTCCC TGGAGAACTT CCTGTGCGAC CCATACTCAG CAGAGAGTCC ATCCCCACAG
    GGTGGCCCAG ATGATGTGAG CTTACTCAGC CCCGGGGAAG ATGTGCTGAT TGACATCGAT
    GACAAGGAGC CTCTCATTCC TGTCCAGCTT ACAGATGGGC TGTCAAGCTT GGGCCCCACT
    AACCTGACGC CTTCTCCATC CAGTCCTCAT GAGGAGGAGT TCATTGACTG GTGGAGCAAA
    TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC
    ACCTTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CTTTTGGAGG CCTGTCCGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AGGACTCAGG AGGAGACAGA TGATCCATCT
    GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC
    CCCATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC
    CGTATTTATA TCGTCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT
    TGCACCCTGG AGCCTGTATT CGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG
    AAGGACCTGA AGATCACACT CTACGATTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG
    GAGACGGTCA TAGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC
    CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG
    CTCCTCCACC TCTTCTGCCA GCAGCACAGG ATCAAGGCCC CCGTGTACCG GACAGACCGA
    GTGACGTTTC AGGATAAGGA CTACACCATT GAGGAGATAG AGGCTGGCAG ACTCCCAAAC
    CCACACCTGG GCCCAGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAGCA ACAAGGCTTG
    GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT TATAGTCCTC TGCAGCCAGA TATCGAGCAG
    GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC
    TTCAACATCA CCCCACGGAA AGCTAGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA
    AAAGATGTGA TCTTGGATGA CCTCAGTCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG
    AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAGCAGAAGA CAGATGTGCA CTACCGCTCC
    CTGGGTGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCTGCT
    GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCCTTCTGGA GACTCGACAA GACAGAGAGC
    AAGATCCCAG CACGTGTGGT CTTCCAGATC TGGGACAATG ACAAATTCTC TTTTGATGAC
    TTTCTGGGCT CTCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT
    GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGA GAAGAAGATG
    TTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTTGCAG AAAGTGAACA TGAAGAGCGG
    CCTGCTGGCC AAGGTCGGGA TGAACCCAAC ATGAATCCGA AGCTAGAGGA TCCAAGGCGC
    CCCGATACTT CTTTCCTGTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCTTGTGG
    CGACGCTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG
    GGTGTCTTTG TCTACGCCTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTTCGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37301
    Clone ID Related Accession (Same CDS sequence) XM_006506173.4 , XM_006506173.3
    Accession Version XM_006506173.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506173.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCACA AGCCACTGAC
    CTGGACGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC
    AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCAG AGCTCAACAC AGGAAAGGGA
    GAAGGTGTGG CTTATCGAGG CAGGGTTCTG TTGTCCCTGG AGACCAAACT GGTGGAACAC
    AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT GATGATATCC TGCGTGTGGA GAAGTACCTG
    CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG
    GATGACGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GGAACAAGTT CGACACCACC
    TGCCTGCCCT TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC
    TATTACTTGC CTTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCCTCATA CTGGGAAGAC
    ATCAGCCATC GAATTGAGAT CCAAAACCAG CTCCTCAGGG TCGCTGACCG CCTGGAAGCT
    AACCTGGAGC AGGTCCACTT GGCTCTGAAG GCACAGTGTT CCTCCGAGGA TGTGGACGCC
    CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC
    CACGAGATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGC TCCGGCTACG CACCCGTCAC
    CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC TTGAAGCTTG GCCATAGTGA GCTCTCCACT
    GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCCGA GGAGCCCCAG
    AACAGCCTGC CGGACATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAG
    CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC
    TGTGGGAAGC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGTATGCC TGGGGCCCGA
    ATGCCCGTGC AGATTCGGAT CAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAATTC
    AATCAGTTTG CTGAGGGAAA GCTCTCTGTC TTTGCCGAAA CGTATGAGAA CCAGACAAAG
    CTGGCACTGG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ACAGGCAAGA TCAAACTACC AAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA
    GGAGATTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ATGCTGACGC TGGTCATCTG
    AGCTTCGTGG AGGAGGTGTT CGAGAACCAG ACCCGGCTCC CTGGGGGCCA GTGGATTTAC
    ATGAGTGATA ACTACACAGA TGTGAATGGG GAGAAGGTAC TTCCGAAGGA TGATATTGAA
    TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT
    GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC AAAACACTGG
    GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG
    AGGGACCTCA GCCAGATGGA AGCACTGAAG AGGCACAGGC AGGCAGAGGC CGAGGGCGAG
    GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGACCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGACTCC
    ATGTCTGTTT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC
    TATGGGAACC GCTACCATCT GCGCTGCTAC CTGTACCAGG CCCGAGACCT GCCAGCCATG
    GACAAGGACT CCTTCTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCATCA AAGCCAGAAG
    ACAGTGGTGG AGAAGAACAC CCTGAATCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG
    ATTGAGATCT TCGGCGAGCC AGCCAGTATC GCCGAGCATC CGCCCTGCAT CGTGGTGGAG
    CTCTATGACC ACGACACCTA TGGTGCTGAC GAGTTCATGG GTCGCTGTAT TTGTCAGCCA
    AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCCGGGGCAG CCAGCCTGCC
    GGCGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC
    CCTGGCTTTG AGATGCATGA AACTTCAAGG ATTTTGGATG AGACGGAGGA CACAGATTTG
    CCTTACCCAC CACCTCAAAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATTAAGCCA
    GCTCTCCAAC GTACTGCCAT TGAGATCCTG GCCTGGGGCC TGCGGAACAT GAAGAGCTAT
    CAGATGGCCA GCATCTCTTC CCCCAGCCTC GTGGTGGAGT GTGGTGGGCA GACAGTGCAG
    TCCTGTGTCA TCAGAAACCT TCGGAAGAAC CCCAACTTTG ATGTCTGTAC TCTCTTCATG
    GAAGTGATGT TACCCAGAGA GGACCTCTAC TGTCCACCTA TTGTGGTCAA GGTCATTGAT
    AATCGCCAGT TTGGCCGCCG GCCAGTTGTG GGTCAGTGTA CCATTCGCTC CCTGGAGAAC
    TTCCTGTGCG ACCCATACTC AGCAGAGAGT CCATCCCCAC AGGGTGGCCC AGATGATGTG
    AGCTTACTCA GCCCCGGGGA AGATGTGCTG ATTGACATCG ATGACAAGGA GCCTCTCATT
    CCTGTCCAGG AGGAGGAGTT CATTGACTGG TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG
    AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG GATTTTGACA CCTTGAAGGT CTATGACACA
    CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC CTGTCCGACT TTTGTAACAC CTTCAAGCTC
    TACCGGGGCA GGACTCAGGA GGAGACAGAT GATCCATCTG TCATCGGAGA ATTTAAGGGT
    CTCTTCAAAA TTTATCCCCT CCCAGAAGAT CCAGCCATCC CCATGCCCCC GAGACAATTC
    CATCAGCTGG CTGCTCAGGG TCCCCAGGAA TGCTTGGTCC GTATTTATAT CGTCCGAGCA
    TTTGGCTTAC AGCCCAAGGA CCCCAATGGC AAGTGTGACC CTTACATCAA GATCTCCATA
    GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCTT GCACCCTGGA GCCTGTATTC
    GGAAAGATGT TTGAACTCAC CTGCACTCTA CCTCTGGAGA AGGACCTGAA GATCACACTC
    TACGATTATG ACCTGCTCTC CAAGGATGAG AAGATTGGGG AGACGGTCAT AGACCTGGAG
    AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGGCTCC CACAGACCTA CTGTGTCTCT
    GGACCAAATC AGTGGAGAGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG
    CAGCACAGGA TCAAGGCCCC CGTGTACCGG ACAGACCGAG TGACGTTTCA GGATAAGGAC
    TACACCATTG AGGAGATAGA GGCTGGCAGA CTCCCAAACC CACACCTGGG CCCAGTGGAG
    GAACGCTTAG CCCTGCATGT CCTTCAGCAA CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA
    CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT ATCGAGCAGG GGAAGCTACA GATGTGGATT
    GACATATTTC CAAAGGTGCT GGGCCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAAA
    GCTAGAAGGT TTTTTCTGCG TTGTATTATT TGGAACACAA AAGATGTGAT CTTGGATGAC
    CTCAGTCTCA CGGGGGAGAA GATGAGTGAC ATCTATGTGA AAGGCTGGAT GGTGGGATTC
    GAAGAACACA AGCAGAAGAC AGATGTGCAC TACCGCTCCC TGGGTGGGGA GGGCAATTTC
    AACTGGAGGT TCGTGTTTCC CTTCGACTAT CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC
    AAGAAGGATG CCTTCTGGAG ACTCGACAAG ACAGAGAGCA AGATCCCAGC ACGTGTGGTC
    TTCCAGATCT GGGACAATGA CAAATTCTCT TTTGATGACT TTCTGGGCTC TCTGCAGCTG
    GATCTCAACC GAATGCCCAA GCCAGCCAAG ACGGCTGAGA AGTGCTCCTT GGACCAGCTG
    GATGACACCT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA
    TGGTGGCCTT GTGTGACAGA GGAGGGCGAG AAGAAGATGT TGGCGGGCAA GCTGGAAATG
    ACCTTGGAGA TTGTTGCAGA AAGTGAACAT GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT
    GAACCCAACA TGAATCCGAA GCTAGAGGAT CCAAGGCGCC CCGATACTTC TTTCCTGTGG
    TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCTTGTGGC GACGCTTCCG GTGTGCCATC
    ATCCTCTTCA TCATCCTCTT CATCCTCCTG CTCTTCCTGG GTGTCTTTGT CTACGCCTTC
    CCGAACTACG CTGCCATGAA GCTGGTGAAG CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506236.1
    CDS555..6896
    Translation

    Target ORF information:

    RefSeq Version XM_006506173.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506173.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCACA AGCCACTGAC
    CTGGACGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC
    AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCAG AGCTCAACAC AGGAAAGGGA
    GAAGGTGTGG CTTATCGAGG CAGGGTTCTG TTGTCCCTGG AGACCAAACT GGTGGAACAC
    AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT GATGATATCC TGCGTGTGGA GAAGTACCTG
    CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG
    GATGACGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GGAACAAGTT CGACACCACC
    TGCCTGCCCT TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC
    TATTACTTGC CTTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCCTCATA CTGGGAAGAC
    ATCAGCCATC GAATTGAGAT CCAAAACCAG CTCCTCAGGG TCGCTGACCG CCTGGAAGCT
    AACCTGGAGC AGGTCCACTT GGCTCTGAAG GCACAGTGTT CCTCCGAGGA TGTGGACGCC
    CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC
    CACGAGATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGC TCCGGCTACG CACCCGTCAC
    CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC TTGAAGCTTG GCCATAGTGA GCTCTCCACT
    GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCCGA GGAGCCCCAG
    AACAGCCTGC CGGACATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAG
    CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC
    TGTGGGAAGC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGTATGCC TGGGGCCCGA
    ATGCCCGTGC AGATTCGGAT CAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAATTC
    AATCAGTTTG CTGAGGGAAA GCTCTCTGTC TTTGCCGAAA CGTATGAGAA CCAGACAAAG
    CTGGCACTGG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ACAGGCAAGA TCAAACTACC AAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA
    GGAGATTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ATGCTGACGC TGGTCATCTG
    AGCTTCGTGG AGGAGGTGTT CGAGAACCAG ACCCGGCTCC CTGGGGGCCA GTGGATTTAC
    ATGAGTGATA ACTACACAGA TGTGAATGGG GAGAAGGTAC TTCCGAAGGA TGATATTGAA
    TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT
    GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC AAAACACTGG
    GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG
    AGGGACCTCA GCCAGATGGA AGCACTGAAG AGGCACAGGC AGGCAGAGGC CGAGGGCGAG
    GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGACCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGACTCC
    ATGTCTGTTT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC
    TATGGGAACC GCTACCATCT GCGCTGCTAC CTGTACCAGG CCCGAGACCT GCCAGCCATG
    GACAAGGACT CCTTCTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCATCA AAGCCAGAAG
    ACAGTGGTGG AGAAGAACAC CCTGAATCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG
    ATTGAGATCT TCGGCGAGCC AGCCAGTATC GCCGAGCATC CGCCCTGCAT CGTGGTGGAG
    CTCTATGACC ACGACACCTA TGGTGCTGAC GAGTTCATGG GTCGCTGTAT TTGTCAGCCA
    AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCCGGGGCAG CCAGCCTGCC
    GGCGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC
    CCTGGCTTTG AGATGCATGA AACTTCAAGG ATTTTGGATG AGACGGAGGA CACAGATTTG
    CCTTACCCAC CACCTCAAAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATTAAGCCA
    GCTCTCCAAC GTACTGCCAT TGAGATCCTG GCCTGGGGCC TGCGGAACAT GAAGAGCTAT
    CAGATGGCCA GCATCTCTTC CCCCAGCCTC GTGGTGGAGT GTGGTGGGCA GACAGTGCAG
    TCCTGTGTCA TCAGAAACCT TCGGAAGAAC CCCAACTTTG ATGTCTGTAC TCTCTTCATG
    GAAGTGATGT TACCCAGAGA GGACCTCTAC TGTCCACCTA TTGTGGTCAA GGTCATTGAT
    AATCGCCAGT TTGGCCGCCG GCCAGTTGTG GGTCAGTGTA CCATTCGCTC CCTGGAGAAC
    TTCCTGTGCG ACCCATACTC AGCAGAGAGT CCATCCCCAC AGGGTGGCCC AGATGATGTG
    AGCTTACTCA GCCCCGGGGA AGATGTGCTG ATTGACATCG ATGACAAGGA GCCTCTCATT
    CCTGTCCAGG AGGAGGAGTT CATTGACTGG TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG
    AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG GATTTTGACA CCTTGAAGGT CTATGACACA
    CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC CTGTCCGACT TTTGTAACAC CTTCAAGCTC
    TACCGGGGCA GGACTCAGGA GGAGACAGAT GATCCATCTG TCATCGGAGA ATTTAAGGGT
    CTCTTCAAAA TTTATCCCCT CCCAGAAGAT CCAGCCATCC CCATGCCCCC GAGACAATTC
    CATCAGCTGG CTGCTCAGGG TCCCCAGGAA TGCTTGGTCC GTATTTATAT CGTCCGAGCA
    TTTGGCTTAC AGCCCAAGGA CCCCAATGGC AAGTGTGACC CTTACATCAA GATCTCCATA
    GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCTT GCACCCTGGA GCCTGTATTC
    GGAAAGATGT TTGAACTCAC CTGCACTCTA CCTCTGGAGA AGGACCTGAA GATCACACTC
    TACGATTATG ACCTGCTCTC CAAGGATGAG AAGATTGGGG AGACGGTCAT AGACCTGGAG
    AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGGCTCC CACAGACCTA CTGTGTCTCT
    GGACCAAATC AGTGGAGAGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG
    CAGCACAGGA TCAAGGCCCC CGTGTACCGG ACAGACCGAG TGACGTTTCA GGATAAGGAC
    TACACCATTG AGGAGATAGA GGCTGGCAGA CTCCCAAACC CACACCTGGG CCCAGTGGAG
    GAACGCTTAG CCCTGCATGT CCTTCAGCAA CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA
    CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT ATCGAGCAGG GGAAGCTACA GATGTGGATT
    GACATATTTC CAAAGGTGCT GGGCCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAAA
    GCTAGAAGGT TTTTTCTGCG TTGTATTATT TGGAACACAA AAGATGTGAT CTTGGATGAC
    CTCAGTCTCA CGGGGGAGAA GATGAGTGAC ATCTATGTGA AAGGCTGGAT GGTGGGATTC
    GAAGAACACA AGCAGAAGAC AGATGTGCAC TACCGCTCCC TGGGTGGGGA GGGCAATTTC
    AACTGGAGGT TCGTGTTTCC CTTCGACTAT CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC
    AAGAAGGATG CCTTCTGGAG ACTCGACAAG ACAGAGAGCA AGATCCCAGC ACGTGTGGTC
    TTCCAGATCT GGGACAATGA CAAATTCTCT TTTGATGACT TTCTGGGCTC TCTGCAGCTG
    GATCTCAACC GAATGCCCAA GCCAGCCAAG ACGGCTGAGA AGTGCTCCTT GGACCAGCTG
    GATGACACCT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA
    TGGTGGCCTT GTGTGACAGA GGAGGGCGAG AAGAAGATGT TGGCGGGCAA GCTGGAAATG
    ACCTTGGAGA TTGTTGCAGA AAGTGAACAT GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT
    GAACCCAACA TGAATCCGAA GCTAGAGGAT CCAAGGCGCC CCGATACTTC TTTCCTGTGG
    TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCTTGTGGC GACGCTTCCG GTGTGCCATC
    ATCCTCTTCA TCATCCTCTT CATCCTCCTG CTCTTCCTGG GTGTCTTTGT CTACGCCTTC
    CCGAACTACG CTGCCATGAA GCTGGTGAAG CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37303
    Clone ID Related Accession (Same CDS sequence) XM_006506175.3 , XM_006506175.4
    Accession Version XM_006506175.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506175.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTACTCAAGA GTCCACAAGC CACTGACCTG GACGACAACC TGGGCTTCCT CCCCACTTTT
    GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT
    TATGCAGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCTT ATCGAGGCAG GGTTCTGTTG
    TCCCTGGAGA CCAAACTGGT GGAACACAGT GAGCAGAAGG TGGAAGACCT TCCTGCTGAT
    GATATCCTGC GTGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC
    TACTCAGCCA CAATGCTGCA GGATGTGGAT GACGCCATCC AGTTCGAGGT CAGCATTGGG
    AACTACGGGA ACAAGTTCGA CACCACCTGC CTGCCCTTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGATGGATG CCACTACTAT TACTTGCCTT GGGGCAACGT GAAGCCCGTG
    GTGGTGCTGT CCTCATACTG GGAAGACATC AGCCATCGAA TTGAGATCCA AAACCAGCTC
    CTCAGGGTCG CTGACCGCCT GGAAGCTAAC CTGGAGCAGG TCCACTTGGC TCTGAAGGCA
    CAGTGTTCCT CCGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA
    GACTGCAGCC AGCCCCTGTG TGACATCCAC GAGATACCTT CTGCCACCCA CCTTGATCAG
    TACCTGCTCC GGCTACGCAC CCGTCACCTG AGCCAGATCA AAGAAGCTGC TCTGGCCTTG
    AAGCTTGGCC ATAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT
    CTCCGTGCCC TGGCCGAGGA GCCCCAGAAC AGCCTGCCGG ACATCATCAT CTGGATGCTG
    CAGGGTGACA AGCGTGTGGC CTACCAGCGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG
    CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAAGCTGC AGACCATCTT TCTGAAATAT
    CCTATGGAAG GTATGCCTGG GGCCCGAATG CCCGTGCAGA TTCGGATCAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAATTCAAT CAGTTTGCTG AGGGAAAGCT CTCTGTCTTT
    GCCGAAACGT ATGAGAACCA GACAAAGCTG GCACTGGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGATGTCACA GGCAAGATCA AACTACCAAA GGATAGCTTC
    CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GATTGGTTCG TGTGTCCAGA GAAGACCCTA
    CTCCATGATG CTGACGCTGG TCATCTGAGC TTCGTGGAGG AGGTGTTCGA GAACCAGACC
    CGGCTCCCTG GGGGCCAGTG GATTTACATG AGTGATAACT ACACAGATGT GAATGGGGAG
    AAGGTACTTC CGAAGGATGA TATTGAATGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA
    CCCCCAGACC GAAAGCCAAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG
    CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAAGC ACTGAAGAGG
    CACAGGCAGG CAGAGGCCGA GGGCGAGGGC TGGGAGTATG CCTCTCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACAGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCGCTGG AGAAGACGGG ACCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    ATGGTGGATG ACAAGAGCGA AGACTCCATG TCTGTTTCTA CCCTGAGCTT TGGCGTTAAC
    AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG CTGCTACCTG
    TACCAGGCCC GAGACCTGCC AGCCATGGAC AAGGACTCCT TCTCTGATCC CTACGCCATC
    GTCTCCTTCC TGCATCAAAG CCAGAAGACA GTGGTGGAGA AGAACACCCT GAATCCCACC
    TGGGATCAAA CCCTCATCTT CTATGAGATT GAGATCTTCG GCGAGCCAGC CAGTATCGCC
    GAGCATCCGC CCTGCATCGT GGTGGAGCTC TATGACCACG ACACCTATGG TGCTGACGAG
    TTCATGGGTC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC
    CCACTGACCC GGGGCAGCCA GCCTGCCGGC GAGCTGCTGG CAGCGTTCGA GCTTATCCAG
    AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCATGAAAC TTCAAGGATT
    TTGGATGAGA CGGAGGACAC AGATTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC
    TACATGGTTC CCCAGAACAT TAAGCCAGCT CTCCAACGTA CTGCCATTGA GATCCTGGCC
    TGGGGCCTGC GGAACATGAA GAGCTATCAG ATGGCCAGCA TCTCTTCCCC CAGCCTCGTG
    GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC
    AACTTTGATG TCTGTACTCT CTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT
    CCACCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCCGGCC AGTTGTGGGT
    CAGTGTACCA TTCGCTCCCT GGAGAACTTC CTGTGCGACC CATACTCAGC AGAGAGTCCA
    TCCCCACAGG GTGGCCCAGA TGATGTGAGC TTACTCAGCC CCGGGGAAGA TGTGCTGATT
    GACATCGATG ACAAGGAGCC TCTCATTCCT GTCCAGCTTA CAGATGGGCT GTCAAGCTTG
    GGCCCCACTA ACCTGACGCC TTCTCCATCC AGTCCTCATG AGGAGGAGTT CATTGACTGG
    TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG
    GATTTTGACA CCTTGAAGGT CTATGACACA CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC
    CTGTCCGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA GGACTCAGGA GGAGACAGAT
    GATCCATCTG TCATCGGAGA ATTTAAGGGT CTCTTCAAAA TTTATCCCCT CCCAGAAGAT
    CCAGCCATCC CCATGCCCCC GAGACAATTC CATCAGCTGG CTGCTCAGGG TCCCCAGGAA
    TGCTTGGTCC GTATTTATAT CGTCCGAGCA TTTGGCTTAC AGCCCAAGGA CCCCAATGGC
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATCCCTT GCACCCTGGA GCCTGTATTC GGAAAGATGT TTGAACTCAC CTGCACTCTA
    CCTCTGGAGA AGGACCTGAA GATCACACTC TACGATTATG ACCTGCTCTC CAAGGATGAG
    AAGATTGGGG AGACGGTCAT AGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGTGGGCTCC CACAGACCTA CTGTGTCTCT GGACCAAATC AGTGGAGAGA TCAGCTCCGC
    CCCTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCACAGGA TCAAGGCCCC CGTGTACCGG
    ACAGACCGAG TGACGTTTCA GGATAAGGAC TACACCATTG AGGAGATAGA GGCTGGCAGA
    CTCCCAAACC CACACCTGGG CCCAGTGGAG GAACGCTTAG CCCTGCATGT CCTTCAGCAA
    CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT
    ATCGAGCAGG GGAAGCTACA GATGTGGATT GACATATTTC CAAAGGTGCT GGGCCGGCCT
    GGACCTCCCT TCAACATCAC CCCACGGAAA GCTAGAAGGT TTTTTCTGCG TTGTATTATT
    TGGAACACAA AAGATGTGAT CTTGGATGAC CTCAGTCTCA CGGGGGAGAA GATGAGTGAC
    ATCTATGTGA AAGGCTGGAT GGTGGGATTC GAAGAACACA AGCAGAAGAC AGATGTGCAC
    TACCGCTCCC TGGGTGGGGA GGGCAATTTC AACTGGAGGT TCGTGTTTCC CTTCGACTAT
    CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC AAGAAGGATG CCTTCTGGAG ACTCGACAAG
    ACAGAGAGCA AGATCCCAGC ACGTGTGGTC TTCCAGATCT GGGACAATGA CAAATTCTCT
    TTTGATGACT TTCTGGGCTC TCTGCAGCTG GATCTCAACC GAATGCCCAA GCCAGCCAAG
    ACGGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACCT TCCACCCAGA ATGGTTTGTG
    TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA TGGTGGCCTT GTGTGACAGA GGAGGGCGAG
    AAGAAGATGT TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TTGTTGCAGA AAGTGAACAT
    GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT GAACCCAACA TGAATCCGAA GCTAGAGGAT
    CCAAGGCGCC CCGATACTTC TTTCCTGTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC
    ATCTTGTGGC GACGCTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTCCTG
    CTCTTCCTGG GTGTCTTTGT CTACGCCTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506238.1
    CDS555..6866
    Translation

    Target ORF information:

    RefSeq Version XM_006506175.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506175.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTACTCAAGA GTCCACAAGC CACTGACCTG GACGACAACC TGGGCTTCCT CCCCACTTTT
    GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT
    TATGCAGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCTT ATCGAGGCAG GGTTCTGTTG
    TCCCTGGAGA CCAAACTGGT GGAACACAGT GAGCAGAAGG TGGAAGACCT TCCTGCTGAT
    GATATCCTGC GTGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC
    TACTCAGCCA CAATGCTGCA GGATGTGGAT GACGCCATCC AGTTCGAGGT CAGCATTGGG
    AACTACGGGA ACAAGTTCGA CACCACCTGC CTGCCCTTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGATGGATG CCACTACTAT TACTTGCCTT GGGGCAACGT GAAGCCCGTG
    GTGGTGCTGT CCTCATACTG GGAAGACATC AGCCATCGAA TTGAGATCCA AAACCAGCTC
    CTCAGGGTCG CTGACCGCCT GGAAGCTAAC CTGGAGCAGG TCCACTTGGC TCTGAAGGCA
    CAGTGTTCCT CCGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA
    GACTGCAGCC AGCCCCTGTG TGACATCCAC GAGATACCTT CTGCCACCCA CCTTGATCAG
    TACCTGCTCC GGCTACGCAC CCGTCACCTG AGCCAGATCA AAGAAGCTGC TCTGGCCTTG
    AAGCTTGGCC ATAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT
    CTCCGTGCCC TGGCCGAGGA GCCCCAGAAC AGCCTGCCGG ACATCATCAT CTGGATGCTG
    CAGGGTGACA AGCGTGTGGC CTACCAGCGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG
    CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAAGCTGC AGACCATCTT TCTGAAATAT
    CCTATGGAAG GTATGCCTGG GGCCCGAATG CCCGTGCAGA TTCGGATCAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAATTCAAT CAGTTTGCTG AGGGAAAGCT CTCTGTCTTT
    GCCGAAACGT ATGAGAACCA GACAAAGCTG GCACTGGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGATGTCACA GGCAAGATCA AACTACCAAA GGATAGCTTC
    CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GATTGGTTCG TGTGTCCAGA GAAGACCCTA
    CTCCATGATG CTGACGCTGG TCATCTGAGC TTCGTGGAGG AGGTGTTCGA GAACCAGACC
    CGGCTCCCTG GGGGCCAGTG GATTTACATG AGTGATAACT ACACAGATGT GAATGGGGAG
    AAGGTACTTC CGAAGGATGA TATTGAATGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA
    CCCCCAGACC GAAAGCCAAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG
    CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAAGC ACTGAAGAGG
    CACAGGCAGG CAGAGGCCGA GGGCGAGGGC TGGGAGTATG CCTCTCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACAGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCGCTGG AGAAGACGGG ACCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    ATGGTGGATG ACAAGAGCGA AGACTCCATG TCTGTTTCTA CCCTGAGCTT TGGCGTTAAC
    AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG CTGCTACCTG
    TACCAGGCCC GAGACCTGCC AGCCATGGAC AAGGACTCCT TCTCTGATCC CTACGCCATC
    GTCTCCTTCC TGCATCAAAG CCAGAAGACA GTGGTGGAGA AGAACACCCT GAATCCCACC
    TGGGATCAAA CCCTCATCTT CTATGAGATT GAGATCTTCG GCGAGCCAGC CAGTATCGCC
    GAGCATCCGC CCTGCATCGT GGTGGAGCTC TATGACCACG ACACCTATGG TGCTGACGAG
    TTCATGGGTC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC
    CCACTGACCC GGGGCAGCCA GCCTGCCGGC GAGCTGCTGG CAGCGTTCGA GCTTATCCAG
    AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCATGAAAC TTCAAGGATT
    TTGGATGAGA CGGAGGACAC AGATTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC
    TACATGGTTC CCCAGAACAT TAAGCCAGCT CTCCAACGTA CTGCCATTGA GATCCTGGCC
    TGGGGCCTGC GGAACATGAA GAGCTATCAG ATGGCCAGCA TCTCTTCCCC CAGCCTCGTG
    GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC
    AACTTTGATG TCTGTACTCT CTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT
    CCACCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCCGGCC AGTTGTGGGT
    CAGTGTACCA TTCGCTCCCT GGAGAACTTC CTGTGCGACC CATACTCAGC AGAGAGTCCA
    TCCCCACAGG GTGGCCCAGA TGATGTGAGC TTACTCAGCC CCGGGGAAGA TGTGCTGATT
    GACATCGATG ACAAGGAGCC TCTCATTCCT GTCCAGCTTA CAGATGGGCT GTCAAGCTTG
    GGCCCCACTA ACCTGACGCC TTCTCCATCC AGTCCTCATG AGGAGGAGTT CATTGACTGG
    TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG
    GATTTTGACA CCTTGAAGGT CTATGACACA CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC
    CTGTCCGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA GGACTCAGGA GGAGACAGAT
    GATCCATCTG TCATCGGAGA ATTTAAGGGT CTCTTCAAAA TTTATCCCCT CCCAGAAGAT
    CCAGCCATCC CCATGCCCCC GAGACAATTC CATCAGCTGG CTGCTCAGGG TCCCCAGGAA
    TGCTTGGTCC GTATTTATAT CGTCCGAGCA TTTGGCTTAC AGCCCAAGGA CCCCAATGGC
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATCCCTT GCACCCTGGA GCCTGTATTC GGAAAGATGT TTGAACTCAC CTGCACTCTA
    CCTCTGGAGA AGGACCTGAA GATCACACTC TACGATTATG ACCTGCTCTC CAAGGATGAG
    AAGATTGGGG AGACGGTCAT AGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGTGGGCTCC CACAGACCTA CTGTGTCTCT GGACCAAATC AGTGGAGAGA TCAGCTCCGC
    CCCTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCACAGGA TCAAGGCCCC CGTGTACCGG
    ACAGACCGAG TGACGTTTCA GGATAAGGAC TACACCATTG AGGAGATAGA GGCTGGCAGA
    CTCCCAAACC CACACCTGGG CCCAGTGGAG GAACGCTTAG CCCTGCATGT CCTTCAGCAA
    CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT
    ATCGAGCAGG GGAAGCTACA GATGTGGATT GACATATTTC CAAAGGTGCT GGGCCGGCCT
    GGACCTCCCT TCAACATCAC CCCACGGAAA GCTAGAAGGT TTTTTCTGCG TTGTATTATT
    TGGAACACAA AAGATGTGAT CTTGGATGAC CTCAGTCTCA CGGGGGAGAA GATGAGTGAC
    ATCTATGTGA AAGGCTGGAT GGTGGGATTC GAAGAACACA AGCAGAAGAC AGATGTGCAC
    TACCGCTCCC TGGGTGGGGA GGGCAATTTC AACTGGAGGT TCGTGTTTCC CTTCGACTAT
    CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC AAGAAGGATG CCTTCTGGAG ACTCGACAAG
    ACAGAGAGCA AGATCCCAGC ACGTGTGGTC TTCCAGATCT GGGACAATGA CAAATTCTCT
    TTTGATGACT TTCTGGGCTC TCTGCAGCTG GATCTCAACC GAATGCCCAA GCCAGCCAAG
    ACGGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACCT TCCACCCAGA ATGGTTTGTG
    TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA TGGTGGCCTT GTGTGACAGA GGAGGGCGAG
    AAGAAGATGT TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TTGTTGCAGA AAGTGAACAT
    GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT GAACCCAACA TGAATCCGAA GCTAGAGGAT
    CCAAGGCGCC CCGATACTTC TTTCCTGTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC
    ATCTTGTGGC GACGCTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTCCTG
    CTCTTCCTGG GTGTCTTTGT CTACGCCTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37305
    Clone ID Related Accession (Same CDS sequence) XM_006506177.4 , XM_006506177.3
    Accession Version XM_006506177.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506177.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGTGGA CGACAACCTG
    GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC
    ACGGGCTTCC CAGATCCTTA TGCAGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCTTAT
    CGAGGCAGGG TTCTGTTGTC CCTGGAGACC AAACTGGTGG AACACAGTGA GCAGAAGGTG
    GAAGACCTTC CTGCTGATGA TATCCTGCGT GTGGAGAAGT ACCTGCGGAG GCGCAAATAT
    TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA CGCCATCCAG
    TTCGAGGTCA GCATTGGGAA CTACGGGAAC AAGTTCGACA CCACCTGCCT GCCCTTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTATTA CTTGCCTTGG
    GGCAACGTGA AGCCCGTGGT GGTGCTGTCC TCATACTGGG AAGACATCAG CCATCGAATT
    GAGATCCAAA ACCAGCTCCT CAGGGTCGCT GACCGCCTGG AAGCTAACCT GGAGCAGGTC
    CACTTGGCTC TGAAGGCACA GTGTTCCTCC GAGGATGTGG ACGCCCTGGT GGCTCAGTTG
    ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCACGA GATACCTTCT
    GCCACCCACC TTGATCAGTA CCTGCTCCGG CTACGCACCC GTCACCTGAG CCAGATCAAA
    GAAGCTGCTC TGGCCTTGAA GCTTGGCCAT AGTGAGCTCT CCACTGCCCT GGAGCAGGCC
    GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCCGAGGAGC CCCAGAACAG CCTGCCGGAC
    ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAGCGGGT GCCTGCCCAT
    GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAAGCTGCAG
    ACCATCTTTC TGAAATATCC TATGGAAGGT ATGCCTGGGG CCCGAATGCC CGTGCAGATT
    CGGATCAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AATTCAATCA GTTTGCTGAG
    GGAAAGCTCT CTGTCTTTGC CGAAACGTAT GAGAACCAGA CAAAGCTGGC ACTGGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ATGTCACAGG CAAGATCAAA
    CTACCAAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA TTGGTTCGTG
    TGTCCAGAGA AGACCCTACT CCATGATGCT GACGCTGGTC ATCTGAGCTT CGTGGAGGAG
    GTGTTCGAGA ACCAGACCCG GCTCCCTGGG GGCCAGTGGA TTTACATGAG TGATAACTAC
    ACAGATGTGA ATGGGGAGAA GGTACTTCCG AAGGATGATA TTGAATGCCC GCTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG
    GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCAAAAC ACTGGGTCCC TGTTGAGAAG
    ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAAGCAC TGAAGAGGCA CAGGCAGGCA GAGGCCGAGG GCGAGGGCTG GGAGTATGCC
    TCTCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGAC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ACTCCATGTC TGTTTCTACC
    CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC
    CATCTGCGCT GCTACCTGTA CCAGGCCCGA GACCTGCCAG CCATGGACAA GGACTCCTTC
    TCTGATCCCT ACGCCATCGT CTCCTTCCTG CATCAAAGCC AGAAGACAGT GGTGGAGAAG
    AACACCCTGA ATCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATTGA GATCTTCGGC
    GAGCCAGCCA GTATCGCCGA GCATCCGCCC TGCATCGTGG TGGAGCTCTA TGACCACGAC
    ACCTATGGTG CTGACGAGTT CATGGGTCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG
    CCCCGCCTGG CCTGGTTCCC ACTGACCCGG GGCAGCCAGC CTGCCGGCGA GCTGCTGGCA
    GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG
    CATGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ATTTGCCTTA CCCACCACCT
    CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATTA AGCCAGCTCT CCAACGTACT
    GCCATTGAGA TCCTGGCCTG GGGCCTGCGG AACATGAAGA GCTATCAGAT GGCCAGCATC
    TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA
    AACCTTCGGA AGAACCCCAA CTTTGATGTC TGTACTCTCT TCATGGAAGT GATGTTACCC
    AGAGAGGACC TCTACTGTCC ACCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC
    CGCCGGCCAG TTGTGGGTCA GTGTACCATT CGCTCCCTGG AGAACTTCCT GTGCGACCCA
    TACTCAGCAG AGAGTCCATC CCCACAGGGT GGCCCAGATG ATGTGAGCTT ACTCAGCCCC
    GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCTC TCATTCCTGT CCAGCTTACA
    GATGGGCTGT CAAGCTTGGG CCCCACTAAC CTGACGCCTT CTCCATCCAG TCCTCATGAG
    GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT
    GGTTCCTACC TGGAGAAGGA TTTTGACACC TTGAAGGTCT ATGACACACA GCTGGAGAAT
    GTGGAGGCTT TTGGAGGCCT GTCCGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAGG
    ACTCAGGAGG AGACAGATGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT
    TATCCCCTCC CAGAAGATCC AGCCATCCCC ATGCCCCCGA GACAATTCCA TCAGCTGGCT
    GCTCAGGGTC CCCAGGAATG CTTGGTCCGT ATTTATATCG TCCGAGCATT TGGCTTACAG
    CCCAAGGACC CCAATGGCAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATCCCTTGC ACCCTGGAGC CTGTATTCGG AAAGATGTTT
    GAACTCACCT GCACTCTACC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGATTATGAC
    CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACGGTCATAG ACCTGGAGAA CAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG
    TGGAGAGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGATC
    AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGACTA CACCATTGAG
    GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CAGTGGAGGA ACGCTTAGCC
    CTGCATGTCC TTCAGCAACA AGGCTTGGTT CCTGAGCATG TGGAGTCACG GCCTCTTTAT
    AGTCCTCTGC AGCCAGATAT CGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA
    AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TAGAAGGTTT
    TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGTCTCACG
    GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAG
    CAGAAGACAG ATGTGCACTA CCGCTCCCTG GGTGGGGAGG GCAATTTCAA CTGGAGGTTC
    GTGTTTCCCT TCGACTATCT GCCTGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCC
    TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATCTGG
    GACAATGACA AATTCTCTTT TGATGACTTT CTGGGCTCTC TGCAGCTGGA TCTCAACCGA
    ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC
    CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT
    GTGACAGAGG AGGGCGAGAA GAAGATGTTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT
    GTTGCAGAAA GTGAACATGA AGAGCGGCCT GCTGGCCAAG GTCGGGATGA ACCCAACATG
    AATCCGAAGC TAGAGGATCC AAGGCGCCCC GATACTTCTT TCCTGTGGTT CACCTCCCCG
    TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGCTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506240.1
    CDS555..6824
    Translation

    Target ORF information:

    RefSeq Version XM_006506177.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506177.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGTGGA CGACAACCTG
    GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC
    ACGGGCTTCC CAGATCCTTA TGCAGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCTTAT
    CGAGGCAGGG TTCTGTTGTC CCTGGAGACC AAACTGGTGG AACACAGTGA GCAGAAGGTG
    GAAGACCTTC CTGCTGATGA TATCCTGCGT GTGGAGAAGT ACCTGCGGAG GCGCAAATAT
    TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA CGCCATCCAG
    TTCGAGGTCA GCATTGGGAA CTACGGGAAC AAGTTCGACA CCACCTGCCT GCCCTTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTATTA CTTGCCTTGG
    GGCAACGTGA AGCCCGTGGT GGTGCTGTCC TCATACTGGG AAGACATCAG CCATCGAATT
    GAGATCCAAA ACCAGCTCCT CAGGGTCGCT GACCGCCTGG AAGCTAACCT GGAGCAGGTC
    CACTTGGCTC TGAAGGCACA GTGTTCCTCC GAGGATGTGG ACGCCCTGGT GGCTCAGTTG
    ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCACGA GATACCTTCT
    GCCACCCACC TTGATCAGTA CCTGCTCCGG CTACGCACCC GTCACCTGAG CCAGATCAAA
    GAAGCTGCTC TGGCCTTGAA GCTTGGCCAT AGTGAGCTCT CCACTGCCCT GGAGCAGGCC
    GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCCGAGGAGC CCCAGAACAG CCTGCCGGAC
    ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAGCGGGT GCCTGCCCAT
    GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAAGCTGCAG
    ACCATCTTTC TGAAATATCC TATGGAAGGT ATGCCTGGGG CCCGAATGCC CGTGCAGATT
    CGGATCAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AATTCAATCA GTTTGCTGAG
    GGAAAGCTCT CTGTCTTTGC CGAAACGTAT GAGAACCAGA CAAAGCTGGC ACTGGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ATGTCACAGG CAAGATCAAA
    CTACCAAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA TTGGTTCGTG
    TGTCCAGAGA AGACCCTACT CCATGATGCT GACGCTGGTC ATCTGAGCTT CGTGGAGGAG
    GTGTTCGAGA ACCAGACCCG GCTCCCTGGG GGCCAGTGGA TTTACATGAG TGATAACTAC
    ACAGATGTGA ATGGGGAGAA GGTACTTCCG AAGGATGATA TTGAATGCCC GCTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG
    GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCAAAAC ACTGGGTCCC TGTTGAGAAG
    ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAAGCAC TGAAGAGGCA CAGGCAGGCA GAGGCCGAGG GCGAGGGCTG GGAGTATGCC
    TCTCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGAC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ACTCCATGTC TGTTTCTACC
    CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC
    CATCTGCGCT GCTACCTGTA CCAGGCCCGA GACCTGCCAG CCATGGACAA GGACTCCTTC
    TCTGATCCCT ACGCCATCGT CTCCTTCCTG CATCAAAGCC AGAAGACAGT GGTGGAGAAG
    AACACCCTGA ATCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATTGA GATCTTCGGC
    GAGCCAGCCA GTATCGCCGA GCATCCGCCC TGCATCGTGG TGGAGCTCTA TGACCACGAC
    ACCTATGGTG CTGACGAGTT CATGGGTCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG
    CCCCGCCTGG CCTGGTTCCC ACTGACCCGG GGCAGCCAGC CTGCCGGCGA GCTGCTGGCA
    GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG
    CATGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ATTTGCCTTA CCCACCACCT
    CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATTA AGCCAGCTCT CCAACGTACT
    GCCATTGAGA TCCTGGCCTG GGGCCTGCGG AACATGAAGA GCTATCAGAT GGCCAGCATC
    TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA
    AACCTTCGGA AGAACCCCAA CTTTGATGTC TGTACTCTCT TCATGGAAGT GATGTTACCC
    AGAGAGGACC TCTACTGTCC ACCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC
    CGCCGGCCAG TTGTGGGTCA GTGTACCATT CGCTCCCTGG AGAACTTCCT GTGCGACCCA
    TACTCAGCAG AGAGTCCATC CCCACAGGGT GGCCCAGATG ATGTGAGCTT ACTCAGCCCC
    GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCTC TCATTCCTGT CCAGCTTACA
    GATGGGCTGT CAAGCTTGGG CCCCACTAAC CTGACGCCTT CTCCATCCAG TCCTCATGAG
    GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT
    GGTTCCTACC TGGAGAAGGA TTTTGACACC TTGAAGGTCT ATGACACACA GCTGGAGAAT
    GTGGAGGCTT TTGGAGGCCT GTCCGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAGG
    ACTCAGGAGG AGACAGATGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT
    TATCCCCTCC CAGAAGATCC AGCCATCCCC ATGCCCCCGA GACAATTCCA TCAGCTGGCT
    GCTCAGGGTC CCCAGGAATG CTTGGTCCGT ATTTATATCG TCCGAGCATT TGGCTTACAG
    CCCAAGGACC CCAATGGCAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATCCCTTGC ACCCTGGAGC CTGTATTCGG AAAGATGTTT
    GAACTCACCT GCACTCTACC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGATTATGAC
    CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACGGTCATAG ACCTGGAGAA CAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG
    TGGAGAGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGATC
    AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGACTA CACCATTGAG
    GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CAGTGGAGGA ACGCTTAGCC
    CTGCATGTCC TTCAGCAACA AGGCTTGGTT CCTGAGCATG TGGAGTCACG GCCTCTTTAT
    AGTCCTCTGC AGCCAGATAT CGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA
    AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TAGAAGGTTT
    TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGTCTCACG
    GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAG
    CAGAAGACAG ATGTGCACTA CCGCTCCCTG GGTGGGGAGG GCAATTTCAA CTGGAGGTTC
    GTGTTTCCCT TCGACTATCT GCCTGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCC
    TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATCTGG
    GACAATGACA AATTCTCTTT TGATGACTTT CTGGGCTCTC TGCAGCTGGA TCTCAACCGA
    ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC
    CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT
    GTGACAGAGG AGGGCGAGAA GAAGATGTTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT
    GTTGCAGAAA GTGAACATGA AGAGCGGCCT GCTGGCCAAG GTCGGGATGA ACCCAACATG
    AATCCGAAGC TAGAGGATCC AAGGCGCCCC GATACTTCTT TCCTGTGGTT CACCTCCCCG
    TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGCTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37307
    Clone ID Related Accession (Same CDS sequence) XM_006506179.3 , XM_006506179.4
    Accession Version XM_006506179.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506179.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTACTCAAGA GTCCACAAGC CACTGACCTG GACGACAACC TGGGCTTCCT CCCCACTTTT
    GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT
    TATGCAGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCTT ATCGAGGCAG GGTTCTGTTG
    TCCCTGGAGA CCAAACTGGT GGAACACAGT GAGCAGAAGG TGGAAGACCT TCCTGCTGAT
    GATATCCTGC GTGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC
    TACTCAGCCA CAATGCTGCA GGATGTGGAT GACGCCATCC AGTTCGAGGT CAGCATTGGG
    AACTACGGGA ACAAGTTCGA CACCACCTGC CTGCCCTTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGATGGATG CCACTACTAT TACTTGCCTT GGGGCAACGT GAAGCCCGTG
    GTGGTGCTGT CCTCATACTG GGAAGACATC AGCCATCGAA TTGAGATCCA AAACCAGCTC
    CTCAGGGTCG CTGACCGCCT GGAAGCTAAC CTGGAGCAGG TCCACTTGGC TCTGAAGGCA
    CAGTGTTCCT CCGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA
    GACTGCAGCC AGCCCCTGTG TGACATCCAC GAGATACCTT CTGCCACCCA CCTTGATCAG
    TACCTGCTCC GGCTACGCAC CCGTCACCTG AGCCAGATCA AAGAAGCTGC TCTGGCCTTG
    AAGCTTGGCC ATAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT
    CTCCGTGCCC TGGCCGAGGA GCCCCAGAAC AGCCTGCCGG ACATCATCAT CTGGATGCTG
    CAGGGTGACA AGCGTGTGGC CTACCAGCGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG
    CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAAGCTGC AGACCATCTT TCTGAAATAT
    CCTATGGAAG GTATGCCTGG GGCCCGAATG CCCGTGCAGA TTCGGATCAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAATTCAAT CAGTTTGCTG AGGGAAAGCT CTCTGTCTTT
    GCCGAAACGT ATGAGAACCA GACAAAGCTG GCACTGGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGATGTCACA GGCAAGATCA AACTACCAAA GGATAGCTTC
    CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GATTGGTTCG TGTGTCCAGA GAAGACCCTA
    CTCCATGATG CTGACGCTGG TCATCTGAGC TTCGTGGAGG AGGTGTTCGA GAACCAGACC
    CGGCTCCCTG GGGGCCAGTG GATTTACATG AGTGATAACT ACACAGATGT GAATGGGGAG
    AAGGTACTTC CGAAGGATGA TATTGAATGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA
    CCCCCAGACC GAAAGCCAAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG
    CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAAGC ACTGAAGAGG
    CACAGGCAGG CAGAGGCCGA GGGCGAGGGC TGGGAGTATG CCTCTCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACAGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCGCTGG AGAAGACGGG ACCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    ATGGTGGATG ACAAGAGCGA AGACTCCATG TCTGTTTCTA CCCTGAGCTT TGGCGTTAAC
    AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG CTGCTACCTG
    TACCAGGCCC GAGACCTGCC AGCCATGGAC AAGGACTCCT TCTCTGATCC CTACGCCATC
    GTCTCCTTCC TGCATCAAAG CCAGAAGACA GTGGTGGAGA AGAACACCCT GAATCCCACC
    TGGGATCAAA CCCTCATCTT CTATGAGATT GAGATCTTCG GCGAGCCAGC CAGTATCGCC
    GAGCATCCGC CCTGCATCGT GGTGGAGCTC TATGACCACG ACACCTATGG TGCTGACGAG
    TTCATGGGTC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC
    CCACTGACCC GGGGCAGCCA GCCTGCCGGC GAGCTGCTGG CAGCGTTCGA GCTTATCCAG
    AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCATGAAAC TTCAAGGATT
    TTGGATGAGA CGGAGGACAC AGATTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC
    TACATGGTTC CCCAGAACAT TAAGCCAGCT CTCCAACGTA CTGCCATTGA GATCCTGGCC
    TGGGGCCTGC GGAACATGAA GAGCTATCAG ATGGCCAGCA TCTCTTCCCC CAGCCTCGTG
    GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC
    AACTTTGATG TCTGTACTCT CTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT
    CCACCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCCGGCC AGTTGTGGGT
    CAGTGTACCA TTCGCTCCCT GGAGAACTTC CTGTGCGACC CATACTCAGC AGAGAGTCCA
    TCCCCACAGG GTGGCCCAGA TGATGTGAGC TTACTCAGCC CCGGGGAAGA TGTGCTGATT
    GACATCGATG ACAAGGAGCC TCTCATTCCT GTCCAGGAGG AGGAGTTCAT TGACTGGTGG
    AGCAAATTCT TTGCTTCTGT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT
    TTTGACACCT TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCTTT TGGAGGCCTG
    TCCGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAGGA CTCAGGAGGA GACAGATGAT
    CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA
    GCCATCCCCA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC
    TTGGTCCGTA TTTATATCGT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG
    TGTGACCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATCCCTTGCA CCCTGGAGCC TGTATTCGGA AAGATGTTTG AACTCACCTG CACTCTACCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAC GATTATGACC TGCTCTCCAA GGATGAGAAG
    ATTGGGGAGA CGGTCATAGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT
    GGGCTCCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA GCTCCGCCCC
    TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGATCA AGGCCCCCGT GTACCGGACA
    GACCGAGTGA CGTTTCAGGA TAAGGACTAC ACCATTGAGG AGATAGAGGC TGGCAGACTC
    CCAAACCCAC ACCTGGGCCC AGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAGCAACAA
    GGCTTGGTTC CTGAGCATGT GGAGTCACGG CCTCTTTATA GTCCTCTGCA GCCAGATATC
    GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA
    CCTCCCTTCA ACATCACCCC ACGGAAAGCT AGAAGGTTTT TTCTGCGTTG TATTATTTGG
    AACACAAAAG ATGTGATCTT GGATGACCTC AGTCTCACGG GGGAGAAGAT GAGTGACATC
    TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAGC AGAAGACAGA TGTGCACTAC
    CGCTCCCTGG GTGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG
    CCTGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCCT TCTGGAGACT CGACAAGACA
    GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATCTGGG ACAATGACAA ATTCTCTTTT
    GATGACTTTC TGGGCTCTCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC
    CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGAGAAG
    AAGATGTTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TTGCAGAAAG TGAACATGAA
    GAGCGGCCTG CTGGCCAAGG TCGGGATGAA CCCAACATGA ATCCGAAGCT AGAGGATCCA
    AGGCGCCCCG ATACTTCTTT CCTGTGGTTC ACCTCCCCGT ACAAGACCAT GAAGTTCATC
    TTGTGGCGAC GCTTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC
    TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506242.1
    CDS555..6803
    Translation

    Target ORF information:

    RefSeq Version XM_006506179.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506179.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTACTCAAGA GTCCACAAGC CACTGACCTG GACGACAACC TGGGCTTCCT CCCCACTTTT
    GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT
    TATGCAGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCTT ATCGAGGCAG GGTTCTGTTG
    TCCCTGGAGA CCAAACTGGT GGAACACAGT GAGCAGAAGG TGGAAGACCT TCCTGCTGAT
    GATATCCTGC GTGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC
    TACTCAGCCA CAATGCTGCA GGATGTGGAT GACGCCATCC AGTTCGAGGT CAGCATTGGG
    AACTACGGGA ACAAGTTCGA CACCACCTGC CTGCCCTTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGATGGATG CCACTACTAT TACTTGCCTT GGGGCAACGT GAAGCCCGTG
    GTGGTGCTGT CCTCATACTG GGAAGACATC AGCCATCGAA TTGAGATCCA AAACCAGCTC
    CTCAGGGTCG CTGACCGCCT GGAAGCTAAC CTGGAGCAGG TCCACTTGGC TCTGAAGGCA
    CAGTGTTCCT CCGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA
    GACTGCAGCC AGCCCCTGTG TGACATCCAC GAGATACCTT CTGCCACCCA CCTTGATCAG
    TACCTGCTCC GGCTACGCAC CCGTCACCTG AGCCAGATCA AAGAAGCTGC TCTGGCCTTG
    AAGCTTGGCC ATAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT
    CTCCGTGCCC TGGCCGAGGA GCCCCAGAAC AGCCTGCCGG ACATCATCAT CTGGATGCTG
    CAGGGTGACA AGCGTGTGGC CTACCAGCGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG
    CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAAGCTGC AGACCATCTT TCTGAAATAT
    CCTATGGAAG GTATGCCTGG GGCCCGAATG CCCGTGCAGA TTCGGATCAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAATTCAAT CAGTTTGCTG AGGGAAAGCT CTCTGTCTTT
    GCCGAAACGT ATGAGAACCA GACAAAGCTG GCACTGGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGATGTCACA GGCAAGATCA AACTACCAAA GGATAGCTTC
    CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GATTGGTTCG TGTGTCCAGA GAAGACCCTA
    CTCCATGATG CTGACGCTGG TCATCTGAGC TTCGTGGAGG AGGTGTTCGA GAACCAGACC
    CGGCTCCCTG GGGGCCAGTG GATTTACATG AGTGATAACT ACACAGATGT GAATGGGGAG
    AAGGTACTTC CGAAGGATGA TATTGAATGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA
    CCCCCAGACC GAAAGCCAAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG
    CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAAGC ACTGAAGAGG
    CACAGGCAGG CAGAGGCCGA GGGCGAGGGC TGGGAGTATG CCTCTCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACAGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCGCTGG AGAAGACGGG ACCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    ATGGTGGATG ACAAGAGCGA AGACTCCATG TCTGTTTCTA CCCTGAGCTT TGGCGTTAAC
    AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG CTGCTACCTG
    TACCAGGCCC GAGACCTGCC AGCCATGGAC AAGGACTCCT TCTCTGATCC CTACGCCATC
    GTCTCCTTCC TGCATCAAAG CCAGAAGACA GTGGTGGAGA AGAACACCCT GAATCCCACC
    TGGGATCAAA CCCTCATCTT CTATGAGATT GAGATCTTCG GCGAGCCAGC CAGTATCGCC
    GAGCATCCGC CCTGCATCGT GGTGGAGCTC TATGACCACG ACACCTATGG TGCTGACGAG
    TTCATGGGTC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC
    CCACTGACCC GGGGCAGCCA GCCTGCCGGC GAGCTGCTGG CAGCGTTCGA GCTTATCCAG
    AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCATGAAAC TTCAAGGATT
    TTGGATGAGA CGGAGGACAC AGATTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC
    TACATGGTTC CCCAGAACAT TAAGCCAGCT CTCCAACGTA CTGCCATTGA GATCCTGGCC
    TGGGGCCTGC GGAACATGAA GAGCTATCAG ATGGCCAGCA TCTCTTCCCC CAGCCTCGTG
    GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC
    AACTTTGATG TCTGTACTCT CTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT
    CCACCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCCGGCC AGTTGTGGGT
    CAGTGTACCA TTCGCTCCCT GGAGAACTTC CTGTGCGACC CATACTCAGC AGAGAGTCCA
    TCCCCACAGG GTGGCCCAGA TGATGTGAGC TTACTCAGCC CCGGGGAAGA TGTGCTGATT
    GACATCGATG ACAAGGAGCC TCTCATTCCT GTCCAGGAGG AGGAGTTCAT TGACTGGTGG
    AGCAAATTCT TTGCTTCTGT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT
    TTTGACACCT TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCTTT TGGAGGCCTG
    TCCGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAGGA CTCAGGAGGA GACAGATGAT
    CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA
    GCCATCCCCA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC
    TTGGTCCGTA TTTATATCGT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG
    TGTGACCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATCCCTTGCA CCCTGGAGCC TGTATTCGGA AAGATGTTTG AACTCACCTG CACTCTACCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAC GATTATGACC TGCTCTCCAA GGATGAGAAG
    ATTGGGGAGA CGGTCATAGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT
    GGGCTCCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA GCTCCGCCCC
    TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGATCA AGGCCCCCGT GTACCGGACA
    GACCGAGTGA CGTTTCAGGA TAAGGACTAC ACCATTGAGG AGATAGAGGC TGGCAGACTC
    CCAAACCCAC ACCTGGGCCC AGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAGCAACAA
    GGCTTGGTTC CTGAGCATGT GGAGTCACGG CCTCTTTATA GTCCTCTGCA GCCAGATATC
    GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA
    CCTCCCTTCA ACATCACCCC ACGGAAAGCT AGAAGGTTTT TTCTGCGTTG TATTATTTGG
    AACACAAAAG ATGTGATCTT GGATGACCTC AGTCTCACGG GGGAGAAGAT GAGTGACATC
    TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAGC AGAAGACAGA TGTGCACTAC
    CGCTCCCTGG GTGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG
    CCTGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCCT TCTGGAGACT CGACAAGACA
    GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATCTGGG ACAATGACAA ATTCTCTTTT
    GATGACTTTC TGGGCTCTCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC
    CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGAGAAG
    AAGATGTTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TTGCAGAAAG TGAACATGAA
    GAGCGGCCTG CTGGCCAAGG TCGGGATGAA CCCAACATGA ATCCGAAGCT AGAGGATCCA
    AGGCGCCCCG ATACTTCTTT CCTGTGGTTC ACCTCCCCGT ACAAGACCAT GAAGTTCATC
    TTGTGGCGAC GCTTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC
    TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37309
    Clone ID Related Accession (Same CDS sequence) XM_006506181.3 , XM_006506181.4
    Accession Version XM_006506181.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506181.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGTGGA CGACAACCTG
    GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC
    ACGGGCTTCC CAGATCCTTA TGCAGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCTTAT
    CGAGGCAGGG TTCTGTTGTC CCTGGAGACC AAACTGGTGG AACACAGTGA GCAGAAGGTG
    GAAGACCTTC CTGCTGATGA TATCCTGCGT GTGGAGAAGT ACCTGCGGAG GCGCAAATAT
    TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA CGCCATCCAG
    TTCGAGGTCA GCATTGGGAA CTACGGGAAC AAGTTCGACA CCACCTGCCT GCCCTTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTATTA CTTGCCTTGG
    GGCAACGTGA AGCCCGTGGT GGTGCTGTCC TCATACTGGG AAGACATCAG CCATCGAATT
    GAGATCCAAA ACCAGCTCCT CAGGGTCGCT GACCGCCTGG AAGCTAACCT GGAGCAGGTC
    CACTTGGCTC TGAAGGCACA GTGTTCCTCC GAGGATGTGG ACGCCCTGGT GGCTCAGTTG
    ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCACGA GATACCTTCT
    GCCACCCACC TTGATCAGTA CCTGCTCCGG CTACGCACCC GTCACCTGAG CCAGATCAAA
    GAAGCTGCTC TGGCCTTGAA GCTTGGCCAT AGTGAGCTCT CCACTGCCCT GGAGCAGGCC
    GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCCGAGGAGC CCCAGAACAG CCTGCCGGAC
    ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAGCGGGT GCCTGCCCAT
    GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAAGCTGCAG
    ACCATCTTTC TGAAATATCC TATGGAAGGT ATGCCTGGGG CCCGAATGCC CGTGCAGATT
    CGGATCAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AATTCAATCA GTTTGCTGAG
    GGAAAGCTCT CTGTCTTTGC CGAAACGTAT GAGAACCAGA CAAAGCTGGC ACTGGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ATGTCACAGG CAAGATCAAA
    CTACCAAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA TTGGTTCGTG
    TGTCCAGAGA AGACCCTACT CCATGATGCT GACGCTGGTC ATCTGAGCTT CGTGGAGGAG
    GTGTTCGAGA ACCAGACCCG GCTCCCTGGG GGCCAGTGGA TTTACATGAG TGATAACTAC
    ACAGATGTGA ATGGGGAGAA GGTACTTCCG AAGGATGATA TTGAATGCCC GCTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG
    GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCAAAAC ACTGGGTCCC TGTTGAGAAG
    ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAAGCAC TGAAGAGGCA CAGGCAGGCA GAGGCCGAGG GCGAGGGCTG GGAGTATGCC
    TCTCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGAC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ACTCCATGTC TGTTTCTACC
    CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC
    CATCTGCGCT GCTACCTGTA CCAGGCCCGA GACCTGCCAG CCATGGACAA GGACTCCTTC
    TCTGATCCCT ACGCCATCGT CTCCTTCCTG CATCAAAGCC AGAAGACAGT GGTGGAGAAG
    AACACCCTGA ATCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATTGA GATCTTCGGC
    GAGCCAGCCA GTATCGCCGA GCATCCGCCC TGCATCGTGG TGGAGCTCTA TGACCACGAC
    ACCTATGGTG CTGACGAGTT CATGGGTCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG
    CCCCGCCTGG CCTGGTTCCC ACTGACCCGG GGCAGCCAGC CTGCCGGCGA GCTGCTGGCA
    GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG
    CATGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ATTTGCCTTA CCCACCACCT
    CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATTA AGCCAGCTCT CCAACGTACT
    GCCATTGAGA TCCTGGCCTG GGGCCTGCGG AACATGAAGA GCTATCAGAT GGCCAGCATC
    TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA
    AACCTTCGGA AGAACCCCAA CTTTGATGTC TGTACTCTCT TCATGGAAGT GATGTTACCC
    AGAGAGGACC TCTACTGTCC ACCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC
    CGCCGGCCAG TTGTGGGTCA GTGTACCATT CGCTCCCTGG AGAACTTCCT GTGCGACCCA
    TACTCAGCAG AGAGTCCATC CCCACAGGGT GGCCCAGATG ATGTGAGCTT ACTCAGCCCC
    GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCTC TCATTCCTGT CCAGGAGGAG
    GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTGTCG GGGAGAGGGA AAAGTGTGGT
    TCCTACCTGG AGAAGGATTT TGACACCTTG AAGGTCTATG ACACACAGCT GGAGAATGTG
    GAGGCTTTTG GAGGCCTGTC CGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAGGACT
    CAGGAGGAGA CAGATGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT
    CCCCTCCCAG AAGATCCAGC CATCCCCATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT
    CAGGGTCCCC AGGAATGCTT GGTCCGTATT TATATCGTCC GAGCATTTGG CTTACAGCCC
    AAGGACCCCA ATGGCAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG
    AGTGACCAGG ATAACTACAT CCCTTGCACC CTGGAGCCTG TATTCGGAAA GATGTTTGAA
    CTCACCTGCA CTCTACCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA TTATGACCTG
    CTCTCCAAGG ATGAGAAGAT TGGGGAGACG GTCATAGACC TGGAGAACAG GCTGCTGTCC
    AAGTTTGGGG CTCGCTGTGG GCTCCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG
    AGAGATCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGATCAAG
    GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGACTACAC CATTGAGGAG
    ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCAG TGGAGGAACG CTTAGCCCTG
    CATGTCCTTC AGCAACAAGG CTTGGTTCCT GAGCATGTGG AGTCACGGCC TCTTTATAGT
    CCTCTGCAGC CAGATATCGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG
    GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTAG AAGGTTTTTT
    CTGCGTTGTA TTATTTGGAA CACAAAAGAT GTGATCTTGG ATGACCTCAG TCTCACGGGG
    GAGAAGATGA GTGACATCTA TGTGAAAGGC TGGATGGTGG GATTCGAAGA ACACAAGCAG
    AAGACAGATG TGCACTACCG CTCCCTGGGT GGGGAGGGCA ATTTCAACTG GAGGTTCGTG
    TTTCCCTTCG ACTATCTGCC TGCTGAGCAA GTCTGTGCTG TTGCCAAGAA GGATGCCTTC
    TGGAGACTCG ACAAGACAGA GAGCAAGATC CCAGCACGTG TGGTCTTCCA GATCTGGGAC
    AATGACAAAT TCTCTTTTGA TGACTTTCTG GGCTCTCTGC AGCTGGATCT CAACCGAATG
    CCCAAGCCAG CCAAGACGGC TGAGAAGTGC TCCTTGGACC AGCTGGATGA CACCTTCCAC
    CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAGACAGTGA AAGGATGGTG GCCTTGTGTG
    ACAGAGGAGG GCGAGAAGAA GATGTTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTT
    GCAGAAAGTG AACATGAAGA GCGGCCTGCT GGCCAAGGTC GGGATGAACC CAACATGAAT
    CCGAAGCTAG AGGATCCAAG GCGCCCCGAT ACTTCTTTCC TGTGGTTCAC CTCCCCGTAC
    AAGACCATGA AGTTCATCTT GTGGCGACGC TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TCCTGCTCTT CCTGGGTGTC TTTGTCTACG CCTTCCCGAA CTACGCTGCC
    ATGAAGCTGG TGAAGCCCTT TCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506244.1
    CDS555..6761
    Translation

    Target ORF information:

    RefSeq Version XM_006506181.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506181.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGTGGA CGACAACCTG
    GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC
    ACGGGCTTCC CAGATCCTTA TGCAGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCTTAT
    CGAGGCAGGG TTCTGTTGTC CCTGGAGACC AAACTGGTGG AACACAGTGA GCAGAAGGTG
    GAAGACCTTC CTGCTGATGA TATCCTGCGT GTGGAGAAGT ACCTGCGGAG GCGCAAATAT
    TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA CGCCATCCAG
    TTCGAGGTCA GCATTGGGAA CTACGGGAAC AAGTTCGACA CCACCTGCCT GCCCTTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTATTA CTTGCCTTGG
    GGCAACGTGA AGCCCGTGGT GGTGCTGTCC TCATACTGGG AAGACATCAG CCATCGAATT
    GAGATCCAAA ACCAGCTCCT CAGGGTCGCT GACCGCCTGG AAGCTAACCT GGAGCAGGTC
    CACTTGGCTC TGAAGGCACA GTGTTCCTCC GAGGATGTGG ACGCCCTGGT GGCTCAGTTG
    ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCACGA GATACCTTCT
    GCCACCCACC TTGATCAGTA CCTGCTCCGG CTACGCACCC GTCACCTGAG CCAGATCAAA
    GAAGCTGCTC TGGCCTTGAA GCTTGGCCAT AGTGAGCTCT CCACTGCCCT GGAGCAGGCC
    GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCCGAGGAGC CCCAGAACAG CCTGCCGGAC
    ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAGCGGGT GCCTGCCCAT
    GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAAGCTGCAG
    ACCATCTTTC TGAAATATCC TATGGAAGGT ATGCCTGGGG CCCGAATGCC CGTGCAGATT
    CGGATCAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AATTCAATCA GTTTGCTGAG
    GGAAAGCTCT CTGTCTTTGC CGAAACGTAT GAGAACCAGA CAAAGCTGGC ACTGGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ATGTCACAGG CAAGATCAAA
    CTACCAAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA TTGGTTCGTG
    TGTCCAGAGA AGACCCTACT CCATGATGCT GACGCTGGTC ATCTGAGCTT CGTGGAGGAG
    GTGTTCGAGA ACCAGACCCG GCTCCCTGGG GGCCAGTGGA TTTACATGAG TGATAACTAC
    ACAGATGTGA ATGGGGAGAA GGTACTTCCG AAGGATGATA TTGAATGCCC GCTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG
    GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCAAAAC ACTGGGTCCC TGTTGAGAAG
    ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAAGCAC TGAAGAGGCA CAGGCAGGCA GAGGCCGAGG GCGAGGGCTG GGAGTATGCC
    TCTCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGAC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ACTCCATGTC TGTTTCTACC
    CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC
    CATCTGCGCT GCTACCTGTA CCAGGCCCGA GACCTGCCAG CCATGGACAA GGACTCCTTC
    TCTGATCCCT ACGCCATCGT CTCCTTCCTG CATCAAAGCC AGAAGACAGT GGTGGAGAAG
    AACACCCTGA ATCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATTGA GATCTTCGGC
    GAGCCAGCCA GTATCGCCGA GCATCCGCCC TGCATCGTGG TGGAGCTCTA TGACCACGAC
    ACCTATGGTG CTGACGAGTT CATGGGTCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG
    CCCCGCCTGG CCTGGTTCCC ACTGACCCGG GGCAGCCAGC CTGCCGGCGA GCTGCTGGCA
    GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG
    CATGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ATTTGCCTTA CCCACCACCT
    CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATTA AGCCAGCTCT CCAACGTACT
    GCCATTGAGA TCCTGGCCTG GGGCCTGCGG AACATGAAGA GCTATCAGAT GGCCAGCATC
    TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA
    AACCTTCGGA AGAACCCCAA CTTTGATGTC TGTACTCTCT TCATGGAAGT GATGTTACCC
    AGAGAGGACC TCTACTGTCC ACCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC
    CGCCGGCCAG TTGTGGGTCA GTGTACCATT CGCTCCCTGG AGAACTTCCT GTGCGACCCA
    TACTCAGCAG AGAGTCCATC CCCACAGGGT GGCCCAGATG ATGTGAGCTT ACTCAGCCCC
    GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCTC TCATTCCTGT CCAGGAGGAG
    GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTGTCG GGGAGAGGGA AAAGTGTGGT
    TCCTACCTGG AGAAGGATTT TGACACCTTG AAGGTCTATG ACACACAGCT GGAGAATGTG
    GAGGCTTTTG GAGGCCTGTC CGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAGGACT
    CAGGAGGAGA CAGATGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT
    CCCCTCCCAG AAGATCCAGC CATCCCCATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT
    CAGGGTCCCC AGGAATGCTT GGTCCGTATT TATATCGTCC GAGCATTTGG CTTACAGCCC
    AAGGACCCCA ATGGCAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG
    AGTGACCAGG ATAACTACAT CCCTTGCACC CTGGAGCCTG TATTCGGAAA GATGTTTGAA
    CTCACCTGCA CTCTACCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA TTATGACCTG
    CTCTCCAAGG ATGAGAAGAT TGGGGAGACG GTCATAGACC TGGAGAACAG GCTGCTGTCC
    AAGTTTGGGG CTCGCTGTGG GCTCCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG
    AGAGATCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGATCAAG
    GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGACTACAC CATTGAGGAG
    ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCAG TGGAGGAACG CTTAGCCCTG
    CATGTCCTTC AGCAACAAGG CTTGGTTCCT GAGCATGTGG AGTCACGGCC TCTTTATAGT
    CCTCTGCAGC CAGATATCGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG
    GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTAG AAGGTTTTTT
    CTGCGTTGTA TTATTTGGAA CACAAAAGAT GTGATCTTGG ATGACCTCAG TCTCACGGGG
    GAGAAGATGA GTGACATCTA TGTGAAAGGC TGGATGGTGG GATTCGAAGA ACACAAGCAG
    AAGACAGATG TGCACTACCG CTCCCTGGGT GGGGAGGGCA ATTTCAACTG GAGGTTCGTG
    TTTCCCTTCG ACTATCTGCC TGCTGAGCAA GTCTGTGCTG TTGCCAAGAA GGATGCCTTC
    TGGAGACTCG ACAAGACAGA GAGCAAGATC CCAGCACGTG TGGTCTTCCA GATCTGGGAC
    AATGACAAAT TCTCTTTTGA TGACTTTCTG GGCTCTCTGC AGCTGGATCT CAACCGAATG
    CCCAAGCCAG CCAAGACGGC TGAGAAGTGC TCCTTGGACC AGCTGGATGA CACCTTCCAC
    CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAGACAGTGA AAGGATGGTG GCCTTGTGTG
    ACAGAGGAGG GCGAGAAGAA GATGTTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTT
    GCAGAAAGTG AACATGAAGA GCGGCCTGCT GGCCAAGGTC GGGATGAACC CAACATGAAT
    CCGAAGCTAG AGGATCCAAG GCGCCCCGAT ACTTCTTTCC TGTGGTTCAC CTCCCCGTAC
    AAGACCATGA AGTTCATCTT GTGGCGACGC TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TCCTGCTCTT CCTGGGTGTC TTTGTCTACG CCTTCCCGAA CTACGCTGCC
    ATGAAGCTGG TGAAGCCCTT TCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu89210
    Clone ID Related Accession (Same CDS sequence) XM_030255407.1
    Accession Version XM_030255407.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGACGGA GGACACAGAT
    TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATTAAG
    CCAGCTCTCC AACGTACTGC CATTGAGATC CTGGCCTGGG GCCTGCGGAA CATGAAGAGC
    TATCAGATGG CCAGCATCTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG
    CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGATGTCTG TACTCTCTTC
    ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCAC CTATTGTGGT CAAGGTCATT
    GATAATCGCC AGTTTGGCCG CCGGCCAGTT GTGGGTCAGT GTACCATTCG CTCCCTGGAG
    AACTTCCTGT GCGACCCATA CTCAGCAGAG AGTCCATCCC CACAGGGTGG CCCAGATGAT
    GTGAGCTTAC TCAGCCCCGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCTCTC
    ATTCCTGTCC AGCTTACAGA TGGGCTGTCA AGCTTGGGCC CCACTAACCT GACGCCTTCT
    CCATCCAGTC CTCATGAGGA GGAGTTCATT GACTGGTGGA GCAAATTCTT TGCTTCTGTC
    GGGGAGAGGG AAAAGTGTGG TTCCTACCTG GAGAAGGATT TTGACACCTT GAAGGTCTAT
    GACACACAGC TGGAGAATGT GGAGGCTTTT GGAGGCCTGT CCGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAGGAC TCAGGAGGAG ACAGATGATC CATCTGTCAT CGGAGAATTT
    AAGGGTCTCT TCAAAATTTA TCCCCTCCCA GAAGATCCAG CCATCCCCAT GCCCCCGAGA
    CAATTCCATC AGCTGGCTGC TCAGGGTCCC CAGGAATGCT TGGTCCGTAT TTATATCGTC
    CGAGCATTTG GCTTACAGCC CAAGGACCCC AATGGCAAGT GTGACCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCTTGCAC CCTGGAGCCT
    GTATTCGGAA AGATGTTTGA ACTCACCTGC ACTCTACCTC TGGAGAAGGA CCTGAAGATC
    ACACTCTACG ATTATGACCT GCTCTCCAAG GATGAGAAGA TTGGGGAGAC GGTCATAGAC
    CTGGAGAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GGCTCCCACA GACCTACTGT
    GTCTCTGGAC CAAATCAGTG GAGAGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC
    TGCCAGCAGC ACAGGATCAA GGCCCCCGTG TACCGGACAG ACCGAGTGAC GTTTCAGGAT
    AAGGACTACA CCATTGAGGA GATAGAGGCT GGCAGACTCC CAAACCCACA CCTGGGCCCA
    GTGGAGGAAC GCTTAGCCCT GCATGTCCTT CAGCAACAAG GCTTGGTTCC TGAGCATGTG
    GAGTCACGGC CTCTTTATAG TCCTCTGCAG CCAGATATCG AGCAGGGGAA GCTACAGATG
    TGGATTGACA TATTTCCAAA GGTGCTGGGC CGGCCTGGAC CTCCCTTCAA CATCACCCCA
    CGGAAAGCTA GAAGGTTTTT TCTGCGTTGT ATTATTTGGA ACACAAAAGA TGTGATCTTG
    GATGACCTCA GTCTCACGGG GGAGAAGATG AGTGACATCT ATGTGAAAGG CTGGATGGTG
    GGATTCGAAG AACACAAGCA GAAGACAGAT GTGCACTACC GCTCCCTGGG TGGGGAGGGC
    AATTTCAACT GGAGGTTCGT GTTTCCCTTC GACTATCTGC CTGCTGAGCA AGTCTGTGCT
    GTTGCCAAGA AGGATGCCTT CTGGAGACTC GACAAGACAG AGAGCAAGAT CCCAGCACGT
    GTGGTCTTCC AGATCTGGGA CAATGACAAA TTCTCTTTTG ATGACTTTCT GGGCTCTCTG
    CAGCTGGATC TCAACCGAAT GCCCAAGCCA GCCAAGACGG CTGAGAAGTG CTCCTTGGAC
    CAGCTGGATG ACACCTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAGACAGTG
    AAAGGATGGT GGCCTTGTGT GACAGAGGAG GGCGAGAAGA AGATGTTGGC GGGCAAGCTG
    GAAATGACCT TGGAGATTGT TGCAGAAAGT GAACATGAAG AGCGGCCTGC TGGCCAAGGT
    CGGGATGAAC CCAACATGAA TCCGAAGCTA GAGGATCCAA GGCGCCCCGA TACTTCTTTC
    CTGTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCT TGTGGCGACG CTTCCGGTGT
    GCCATCATCC TCTTCATCAT CCTCTTCATC CTCCTGCTCT TCCTGGGTGT CTTTGTCTAC
    GCCTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030111267.1
    CDS255..6662
    Translation

    Target ORF information:

    RefSeq Version XM_030255407.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030255407.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGACGGA GGACACAGAT
    TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATTAAG
    CCAGCTCTCC AACGTACTGC CATTGAGATC CTGGCCTGGG GCCTGCGGAA CATGAAGAGC
    TATCAGATGG CCAGCATCTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG
    CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGATGTCTG TACTCTCTTC
    ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCAC CTATTGTGGT CAAGGTCATT
    GATAATCGCC AGTTTGGCCG CCGGCCAGTT GTGGGTCAGT GTACCATTCG CTCCCTGGAG
    AACTTCCTGT GCGACCCATA CTCAGCAGAG AGTCCATCCC CACAGGGTGG CCCAGATGAT
    GTGAGCTTAC TCAGCCCCGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCTCTC
    ATTCCTGTCC AGCTTACAGA TGGGCTGTCA AGCTTGGGCC CCACTAACCT GACGCCTTCT
    CCATCCAGTC CTCATGAGGA GGAGTTCATT GACTGGTGGA GCAAATTCTT TGCTTCTGTC
    GGGGAGAGGG AAAAGTGTGG TTCCTACCTG GAGAAGGATT TTGACACCTT GAAGGTCTAT
    GACACACAGC TGGAGAATGT GGAGGCTTTT GGAGGCCTGT CCGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAGGAC TCAGGAGGAG ACAGATGATC CATCTGTCAT CGGAGAATTT
    AAGGGTCTCT TCAAAATTTA TCCCCTCCCA GAAGATCCAG CCATCCCCAT GCCCCCGAGA
    CAATTCCATC AGCTGGCTGC TCAGGGTCCC CAGGAATGCT TGGTCCGTAT TTATATCGTC
    CGAGCATTTG GCTTACAGCC CAAGGACCCC AATGGCAAGT GTGACCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCTTGCAC CCTGGAGCCT
    GTATTCGGAA AGATGTTTGA ACTCACCTGC ACTCTACCTC TGGAGAAGGA CCTGAAGATC
    ACACTCTACG ATTATGACCT GCTCTCCAAG GATGAGAAGA TTGGGGAGAC GGTCATAGAC
    CTGGAGAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GGCTCCCACA GACCTACTGT
    GTCTCTGGAC CAAATCAGTG GAGAGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC
    TGCCAGCAGC ACAGGATCAA GGCCCCCGTG TACCGGACAG ACCGAGTGAC GTTTCAGGAT
    AAGGACTACA CCATTGAGGA GATAGAGGCT GGCAGACTCC CAAACCCACA CCTGGGCCCA
    GTGGAGGAAC GCTTAGCCCT GCATGTCCTT CAGCAACAAG GCTTGGTTCC TGAGCATGTG
    GAGTCACGGC CTCTTTATAG TCCTCTGCAG CCAGATATCG AGCAGGGGAA GCTACAGATG
    TGGATTGACA TATTTCCAAA GGTGCTGGGC CGGCCTGGAC CTCCCTTCAA CATCACCCCA
    CGGAAAGCTA GAAGGTTTTT TCTGCGTTGT ATTATTTGGA ACACAAAAGA TGTGATCTTG
    GATGACCTCA GTCTCACGGG GGAGAAGATG AGTGACATCT ATGTGAAAGG CTGGATGGTG
    GGATTCGAAG AACACAAGCA GAAGACAGAT GTGCACTACC GCTCCCTGGG TGGGGAGGGC
    AATTTCAACT GGAGGTTCGT GTTTCCCTTC GACTATCTGC CTGCTGAGCA AGTCTGTGCT
    GTTGCCAAGA AGGATGCCTT CTGGAGACTC GACAAGACAG AGAGCAAGAT CCCAGCACGT
    GTGGTCTTCC AGATCTGGGA CAATGACAAA TTCTCTTTTG ATGACTTTCT GGGCTCTCTG
    CAGCTGGATC TCAACCGAAT GCCCAAGCCA GCCAAGACGG CTGAGAAGTG CTCCTTGGAC
    CAGCTGGATG ACACCTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAGACAGTG
    AAAGGATGGT GGCCTTGTGT GACAGAGGAG GGCGAGAAGA AGATGTTGGC GGGCAAGCTG
    GAAATGACCT TGGAGATTGT TGCAGAAAGT GAACATGAAG AGCGGCCTGC TGGCCAAGGT
    CGGGATGAAC CCAACATGAA TCCGAAGCTA GAGGATCCAA GGCGCCCCGA TACTTCTTTC
    CTGTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCT TGTGGCGACG CTTCCGGTGT
    GCCATCATCC TCTTCATCAT CCTCTTCATC CTCCTGCTCT TCCTGGGTGT CTTTGTCTAC
    GCCTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16347
    Clone ID Related Accession (Same CDS sequence) NM_001077694.2
    Accession Version NM_001077694.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Mus musculus(house mouse)
    Product dysferlin isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153607.15, BQ831003.1 and AK131144.1. On Oct 26, 2013 this sequence version replaced NM_001077694.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and differs in the 5' UTR and the 5' coding region, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGT GGACGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC
    TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCAGAG
    CTCAACACAG GAAAGGGAGA AGGTGTGGCT TATCGAGGCA GGGTTCTGTT GTCCCTGGAG
    ACCAAACTGG TGGAACACAG TGAGCAGAAG GTGGAAGACC TTCCTGCTGA TGATATCCTG
    CGTGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC
    ACAATGCTGC AGGATGTGGA TGACGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGG
    AACAAGTTCG ACACCACCTG CCTGCCCTTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGATGGAT GCCACTACTA TTACTTGCCT TGGGGCAACG TGAAGCCCGT GGTGGTGCTG
    TCCTCATACT GGGAAGACAT CAGCCATCGA ATTGAGATCC AAAACCAGCT CCTCAGGGTC
    GCTGACCGCC TGGAAGCTAA CCTGGAGCAG GTCCACTTGG CTCTGAAGGC ACAGTGTTCC
    TCCGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC
    CAGCCCCTGT GTGACATCCA CGAGATACCT TCTGCCACCC ACCTTGATCA GTACCTGCTC
    CGGCTACGCA CCCGTCACCT GAGCCAGATC AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC
    CATAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC
    CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG GACATCATCA TCTGGATGCT GCAGGGTGAC
    AAGCGTGTGG CCTACCAGCG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC
    AGCTACTGCG GCAGGAACTG TGGGAAGCTG CAGACCATCT TTCTGAAATA TCCTATGGAA
    GGTATGCCTG GGGCCCGAAT GCCCGTGCAG ATTCGGATCA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAATTCAA TCAGTTTGCT GAGGGAAAGC TCTCTGTCTT TGCCGAAACG
    TATGAGAACC AGACAAAGCT GGCACTGGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGATGTCAC AGGCAAGATC AAACTACCAA AGGATAGCTT CCGCCCCTCA
    GCTGGCTGGG CCTGGGCAGG AGATTGGTTC GTGTGTCCAG AGAAGACCCT ACTCCATGAT
    GCTGACGCTG GTCATCTGAG CTTCGTGGAG GAGGTGTTCG AGAACCAGAC CCGGCTCCCT
    GGGGGCCAGT GGATTTACAT GAGTGATAAC TACACAGATG TGAATGGGGA GAAGGTACTT
    CCGAAGGATG ATATTGAATG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC
    CGAAAGCCAA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC
    TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAAG CACTGAAGAG GCACAGGCAG
    GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT GCCTCTCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACAGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG
    GAGAAGACGG GACCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CATGGTGGAT
    GACAAGAGCG AAGACTCCAT GTCTGTTTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA
    ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GCTGCTACCT GTACCAGGCC
    CGAGACCTGC CAGCCATGGA CAAGGACTCC TTCTCTGATC CCTACGCCAT CGTCTCCTTC
    CTGCATCAAA GCCAGAAGAC AGTGGTGGAG AAGAACACCC TGAATCCCAC CTGGGATCAA
    ACCCTCATCT TCTATGAGAT TGAGATCTTC GGCGAGCCAG CCAGTATCGC CGAGCATCCG
    CCCTGCATCG TGGTGGAGCT CTATGACCAC GACACCTATG GTGCTGACGA GTTCATGGGT
    CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC
    CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG
    CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCATGAAA CTTCAAGGAT TTTGGATGAG
    ACGGAGGACA CAGATTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TTAAGCCAGC TCTCCAACGT ACTGCCATTG AGATCCTGGC CTGGGGCCTG
    CGGAACATGA AGAGCTATCA GATGGCCAGC ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT
    GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAT
    GTCTGTACTC TCTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCACCTATT
    GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCCGGC CAGTTGTGGG TCAGTGTACC
    ATTCGCTCCC TGGAGAACTT CCTGTGCGAC CCATACTCAG CAGAGAGTCC ATCCCCACAG
    GGTGGCCCAG ATGATGTGAG CTTACTCAGC CCCGGGGAAG ATGTGCTGAT TGACATCGAT
    GACAAGGAGC CTCTCATTCC TGTCCAGGAG GAGGAGTTCA TTGACTGGTG GAGCAAATTC
    TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT GGTTCCTACC TGGAGAAGGA TTTTGACACC
    TTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCTT TTGGAGGCCT GTCCGACTTT
    TGTAACACCT TCAAGCTCTA CCGGGGCAGG ACTCAGGAGG AGACAGATGA TCCATCTGTC
    ATCGGAGAAT TTAAGGGTCT CTTCAAAATT TATCCCCTCC CAGAAGATCC AGCCATCCCC
    ATGCCCCCGA GACAATTCCA TCAGCTGGCT GCTCAGGGTC CCCAGGAATG CTTGGTCCGT
    ATTTATATCG TCCGAGCATT TGGCTTACAG CCCAAGGACC CCAATGGCAA GTGTGACCCT
    TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCTTGC
    ACCCTGGAGC CTGTATTCGG AAAGATGTTT GAACTCACCT GCACTCTACC TCTGGAGAAG
    GACCTGAAGA TCACACTCTA CGATTATGAC CTGCTCTCCA AGGATGAGAA GATTGGGGAG
    ACGGTCATAG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA
    CAGACCTACT GTGTCTCTGG ACCAAATCAG TGGAGAGATC AGCTCCGCCC CTCCCAGCTC
    CTCCACCTCT TCTGCCAGCA GCACAGGATC AAGGCCCCCG TGTACCGGAC AGACCGAGTG
    ACGTTTCAGG ATAAGGACTA CACCATTGAG GAGATAGAGG CTGGCAGACT CCCAAACCCA
    CACCTGGGCC CAGTGGAGGA ACGCTTAGCC CTGCATGTCC TTCAGCAACA AGGCTTGGTT
    CCTGAGCATG TGGAGTCACG GCCTCTTTAT AGTCCTCTGC AGCCAGATAT CGAGCAGGGG
    AAGCTACAGA TGTGGATTGA CATATTTCCA AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC
    AACATCACCC CACGGAAAGC TAGAAGGTTT TTTCTGCGTT GTATTATTTG GAACACAAAA
    GATGTGATCT TGGATGACCT CAGTCTCACG GGGGAGAAGA TGAGTGACAT CTATGTGAAA
    GGCTGGATGG TGGGATTCGA AGAACACAAG CAGAAGACAG ATGTGCACTA CCGCTCCCTG
    GGTGGGGAGG GCAATTTCAA CTGGAGGTTC GTGTTTCCCT TCGACTATCT GCCTGCTGAG
    CAAGTCTGTG CTGTTGCCAA GAAGGATGCC TTCTGGAGAC TCGACAAGAC AGAGAGCAAG
    ATCCCAGCAC GTGTGGTCTT CCAGATCTGG GACAATGACA AATTCTCTTT TGATGACTTT
    CTGGGCTCTC TGCAGCTGGA TCTCAACCGA ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG
    TGCTCCTTGG ACCAGCTGGA TGACACCTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG
    CAGAAGACAG TGAAAGGATG GTGGCCTTGT GTGACAGAGG AGGGCGAGAA GAAGATGTTG
    GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTTGCAGAAA GTGAACATGA AGAGCGGCCT
    GCTGGCCAAG GTCGGGATGA ACCCAACATG AATCCGAAGC TAGAGGATCC AAGGCGCCCC
    GATACTTCTT TCCTGTGGTT CACCTCCCCG TACAAGACCA TGAAGTTCAT CTTGTGGCGA
    CGCTTCCGGT GTGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT
    GTCTTTGTCT ACGCCTTCCC GAACTACGCT GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001071162.1
    CDS318..6617
    Translation

    Target ORF information:

    RefSeq Version NM_001077694.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001077694.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGT GGACGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC
    TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCAGAG
    CTCAACACAG GAAAGGGAGA AGGTGTGGCT TATCGAGGCA GGGTTCTGTT GTCCCTGGAG
    ACCAAACTGG TGGAACACAG TGAGCAGAAG GTGGAAGACC TTCCTGCTGA TGATATCCTG
    CGTGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC
    ACAATGCTGC AGGATGTGGA TGACGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGG
    AACAAGTTCG ACACCACCTG CCTGCCCTTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGATGGAT GCCACTACTA TTACTTGCCT TGGGGCAACG TGAAGCCCGT GGTGGTGCTG
    TCCTCATACT GGGAAGACAT CAGCCATCGA ATTGAGATCC AAAACCAGCT CCTCAGGGTC
    GCTGACCGCC TGGAAGCTAA CCTGGAGCAG GTCCACTTGG CTCTGAAGGC ACAGTGTTCC
    TCCGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC
    CAGCCCCTGT GTGACATCCA CGAGATACCT TCTGCCACCC ACCTTGATCA GTACCTGCTC
    CGGCTACGCA CCCGTCACCT GAGCCAGATC AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC
    CATAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC
    CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG GACATCATCA TCTGGATGCT GCAGGGTGAC
    AAGCGTGTGG CCTACCAGCG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC
    AGCTACTGCG GCAGGAACTG TGGGAAGCTG CAGACCATCT TTCTGAAATA TCCTATGGAA
    GGTATGCCTG GGGCCCGAAT GCCCGTGCAG ATTCGGATCA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAATTCAA TCAGTTTGCT GAGGGAAAGC TCTCTGTCTT TGCCGAAACG
    TATGAGAACC AGACAAAGCT GGCACTGGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGATGTCAC AGGCAAGATC AAACTACCAA AGGATAGCTT CCGCCCCTCA
    GCTGGCTGGG CCTGGGCAGG AGATTGGTTC GTGTGTCCAG AGAAGACCCT ACTCCATGAT
    GCTGACGCTG GTCATCTGAG CTTCGTGGAG GAGGTGTTCG AGAACCAGAC CCGGCTCCCT
    GGGGGCCAGT GGATTTACAT GAGTGATAAC TACACAGATG TGAATGGGGA GAAGGTACTT
    CCGAAGGATG ATATTGAATG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC
    CGAAAGCCAA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC
    TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAAG CACTGAAGAG GCACAGGCAG
    GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT GCCTCTCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACAGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG
    GAGAAGACGG GACCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CATGGTGGAT
    GACAAGAGCG AAGACTCCAT GTCTGTTTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA
    ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GCTGCTACCT GTACCAGGCC
    CGAGACCTGC CAGCCATGGA CAAGGACTCC TTCTCTGATC CCTACGCCAT CGTCTCCTTC
    CTGCATCAAA GCCAGAAGAC AGTGGTGGAG AAGAACACCC TGAATCCCAC CTGGGATCAA
    ACCCTCATCT TCTATGAGAT TGAGATCTTC GGCGAGCCAG CCAGTATCGC CGAGCATCCG
    CCCTGCATCG TGGTGGAGCT CTATGACCAC GACACCTATG GTGCTGACGA GTTCATGGGT
    CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC
    CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG
    CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCATGAAA CTTCAAGGAT TTTGGATGAG
    ACGGAGGACA CAGATTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TTAAGCCAGC TCTCCAACGT ACTGCCATTG AGATCCTGGC CTGGGGCCTG
    CGGAACATGA AGAGCTATCA GATGGCCAGC ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT
    GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAT
    GTCTGTACTC TCTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCACCTATT
    GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCCGGC CAGTTGTGGG TCAGTGTACC
    ATTCGCTCCC TGGAGAACTT CCTGTGCGAC CCATACTCAG CAGAGAGTCC ATCCCCACAG
    GGTGGCCCAG ATGATGTGAG CTTACTCAGC CCCGGGGAAG ATGTGCTGAT TGACATCGAT
    GACAAGGAGC CTCTCATTCC TGTCCAGGAG GAGGAGTTCA TTGACTGGTG GAGCAAATTC
    TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT GGTTCCTACC TGGAGAAGGA TTTTGACACC
    TTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCTT TTGGAGGCCT GTCCGACTTT
    TGTAACACCT TCAAGCTCTA CCGGGGCAGG ACTCAGGAGG AGACAGATGA TCCATCTGTC
    ATCGGAGAAT TTAAGGGTCT CTTCAAAATT TATCCCCTCC CAGAAGATCC AGCCATCCCC
    ATGCCCCCGA GACAATTCCA TCAGCTGGCT GCTCAGGGTC CCCAGGAATG CTTGGTCCGT
    ATTTATATCG TCCGAGCATT TGGCTTACAG CCCAAGGACC CCAATGGCAA GTGTGACCCT
    TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCTTGC
    ACCCTGGAGC CTGTATTCGG AAAGATGTTT GAACTCACCT GCACTCTACC TCTGGAGAAG
    GACCTGAAGA TCACACTCTA CGATTATGAC CTGCTCTCCA AGGATGAGAA GATTGGGGAG
    ACGGTCATAG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA
    CAGACCTACT GTGTCTCTGG ACCAAATCAG TGGAGAGATC AGCTCCGCCC CTCCCAGCTC
    CTCCACCTCT TCTGCCAGCA GCACAGGATC AAGGCCCCCG TGTACCGGAC AGACCGAGTG
    ACGTTTCAGG ATAAGGACTA CACCATTGAG GAGATAGAGG CTGGCAGACT CCCAAACCCA
    CACCTGGGCC CAGTGGAGGA ACGCTTAGCC CTGCATGTCC TTCAGCAACA AGGCTTGGTT
    CCTGAGCATG TGGAGTCACG GCCTCTTTAT AGTCCTCTGC AGCCAGATAT CGAGCAGGGG
    AAGCTACAGA TGTGGATTGA CATATTTCCA AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC
    AACATCACCC CACGGAAAGC TAGAAGGTTT TTTCTGCGTT GTATTATTTG GAACACAAAA
    GATGTGATCT TGGATGACCT CAGTCTCACG GGGGAGAAGA TGAGTGACAT CTATGTGAAA
    GGCTGGATGG TGGGATTCGA AGAACACAAG CAGAAGACAG ATGTGCACTA CCGCTCCCTG
    GGTGGGGAGG GCAATTTCAA CTGGAGGTTC GTGTTTCCCT TCGACTATCT GCCTGCTGAG
    CAAGTCTGTG CTGTTGCCAA GAAGGATGCC TTCTGGAGAC TCGACAAGAC AGAGAGCAAG
    ATCCCAGCAC GTGTGGTCTT CCAGATCTGG GACAATGACA AATTCTCTTT TGATGACTTT
    CTGGGCTCTC TGCAGCTGGA TCTCAACCGA ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG
    TGCTCCTTGG ACCAGCTGGA TGACACCTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG
    CAGAAGACAG TGAAAGGATG GTGGCCTTGT GTGACAGAGG AGGGCGAGAA GAAGATGTTG
    GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTTGCAGAAA GTGAACATGA AGAGCGGCCT
    GCTGGCCAAG GTCGGGATGA ACCCAACATG AATCCGAAGC TAGAGGATCC AAGGCGCCCC
    GATACTTCTT TCCTGTGGTT CACCTCCCCG TACAAGACCA TGAAGTTCAT CTTGTGGCGA
    CGCTTCCGGT GTGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT
    GTCTTTGTCT ACGCCTTCCC GAACTACGCT GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16405
    Clone ID Related Accession (Same CDS sequence) NM_021469.3
    Accession Version NM_021469.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Mus musculus(house mouse)
    Product dysferlin isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN716462.1 and AK131144.1. On Oct 26, 2013 this sequence version replaced NM_021469.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131144.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGTGGACGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC
    TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCA
    GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCTTATCGAG GCAGGGTTCT GTTGTCCCTG
    GAGACCAAAC TGGTGGAACA CAGTGAGCAG AAGGTGGAAG ACCTTCCTGC TGATGATATC
    CTGCGTGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA
    GCCACAATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC
    GGGAACAAGT TCGACACCAC CTGCCTGCCC TTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGATG GATGCCACTA CTATTACTTG CCTTGGGGCA ACGTGAAGCC CGTGGTGGTG
    CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAATTGAGA TCCAAAACCA GCTCCTCAGG
    GTCGCTGACC GCCTGGAAGC TAACCTGGAG CAGGTCCACT TGGCTCTGAA GGCACAGTGT
    TCCTCCGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC
    AGCCAGCCCC TGTGTGACAT CCACGAGATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG
    CTCCGGCTAC GCACCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCTCTGGC CTTGAAGCTT
    GGCCATAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT
    GCCCTGGCCG AGGAGCCCCA GAACAGCCTG CCGGACATCA TCATCTGGAT GCTGCAGGGT
    GACAAGCGTG TGGCCTACCA GCGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC
    CCCAGCTACT GCGGCAGGAA CTGTGGGAAG CTGCAGACCA TCTTTCTGAA ATATCCTATG
    GAAGGTATGC CTGGGGCCCG AATGCCCGTG CAGATTCGGA TCAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAATT CAATCAGTTT GCTGAGGGAA AGCTCTCTGT CTTTGCCGAA
    ACGTATGAGA ACCAGACAAA GCTGGCACTG GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGATGT CACAGGCAAG ATCAAACTAC CAAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGGCCTGGGC AGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GATGCTGACG CTGGTCATCT GAGCTTCGTG GAGGAGGTGT TCGAGAACCA GACCCGGCTC
    CCTGGGGGCC AGTGGATTTA CATGAGTGAT AACTACACAG ATGTGAATGG GGAGAAGGTA
    CTTCCGAAGG ATGATATTGA ATGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA
    GACCGAAAGC CAAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA
    CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AAGCACTGAA GAGGCACAGG
    CAGGCAGAGG CCGAGGGCGA GGGCTGGGAG TATGCCTCTC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCG
    CTGGAGAAGA CGGGACCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCATGGTG
    GATGACAAGA GCGAAGACTC CATGTCTGTT TCTACCCTGA GCTTTGGCGT TAACAGACCC
    ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGCTGCTA CCTGTACCAG
    GCCCGAGACC TGCCAGCCAT GGACAAGGAC TCCTTCTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCATC AAAGCCAGAA GACAGTGGTG GAGAAGAACA CCCTGAATCC CACCTGGGAT
    CAAACCCTCA TCTTCTATGA GATTGAGATC TTCGGCGAGC CAGCCAGTAT CGCCGAGCAT
    CCGCCCTGCA TCGTGGTGGA GCTCTATGAC CACGACACCT ATGGTGCTGA CGAGTTCATG
    GGTCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG
    ACCCGGGGCA GCCAGCCTGC CGGCGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG
    AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCATG AAACTTCAAG GATTTTGGAT
    GAGACGGAGG ACACAGATTT GCCTTACCCA CCACCTCAAA GGGAGGCCAA CATCTACATG
    GTTCCCCAGA ACATTAAGCC AGCTCTCCAA CGTACTGCCA TTGAGATCCT GGCCTGGGGC
    CTGCGGAACA TGAAGAGCTA TCAGATGGCC AGCATCTCTT CCCCCAGCCT CGTGGTGGAG
    TGTGGTGGGC AGACAGTGCA GTCCTGTGTC ATCAGAAACC TTCGGAAGAA CCCCAACTTT
    GATGTCTGTA CTCTCTTCAT GGAAGTGATG TTACCCAGAG AGGACCTCTA CTGTCCACCT
    ATTGTGGTCA AGGTCATTGA TAATCGCCAG TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT
    ACCATTCGCT CCCTGGAGAA CTTCCTGTGC GACCCATACT CAGCAGAGAG TCCATCCCCA
    CAGGGTGGCC CAGATGATGT GAGCTTACTC AGCCCCGGGG AAGATGTGCT GATTGACATC
    GATGACAAGG AGCCTCTCAT TCCTGTCCAG GAGGAGGAGT TCATTGACTG GTGGAGCAAA
    TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC
    ACCTTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CTTTTGGAGG CCTGTCCGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AGGACTCAGG AGGAGACAGA TGATCCATCT
    GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC
    CCCATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC
    CGTATTTATA TCGTCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT
    TGCACCCTGG AGCCTGTATT CGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG
    AAGGACCTGA AGATCACACT CTACGATTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG
    GAGACGGTCA TAGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC
    CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG
    CTCCTCCACC TCTTCTGCCA GCAGCACAGG ATCAAGGCCC CCGTGTACCG GACAGACCGA
    GTGACGTTTC AGGATAAGGA CTACACCATT GAGGAGATAG AGGCTGGCAG ACTCCCAAAC
    CCACACCTGG GCCCAGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAGCA ACAAGGCTTG
    GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT TATAGTCCTC TGCAGCCAGA TATCGAGCAG
    GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC
    TTCAACATCA CCCCACGGAA AGCTAGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA
    AAAGATGTGA TCTTGGATGA CCTCAGTCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG
    AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAGCAGAAGA CAGATGTGCA CTACCGCTCC
    CTGGGTGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCTGCT
    GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCCTTCTGGA GACTCGACAA GACAGAGAGC
    AAGATCCCAG CACGTGTGGT CTTCCAGATC TGGGACAATG ACAAATTCTC TTTTGATGAC
    TTTCTGGGCT CTCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT
    GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGA GAAGAAGATG
    TTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTTGCAG AAAGTGAACA TGAAGAGCGG
    CCTGCTGGCC AAGGTCGGGA TGAACCCAAC ATGAATCCGA AGCTAGAGGA TCCAAGGCGC
    CCCGATACTT CTTTCCTGTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCTTGTGG
    CGACGCTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG
    GGTGTCTTTG TCTACGCCTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTTCGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_067444.2
    CDS133..6435
    Translation

    Target ORF information:

    RefSeq Version NM_021469.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_021469.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGTGGACGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC
    TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCA
    GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCTTATCGAG GCAGGGTTCT GTTGTCCCTG
    GAGACCAAAC TGGTGGAACA CAGTGAGCAG AAGGTGGAAG ACCTTCCTGC TGATGATATC
    CTGCGTGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA
    GCCACAATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC
    GGGAACAAGT TCGACACCAC CTGCCTGCCC TTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGATG GATGCCACTA CTATTACTTG CCTTGGGGCA ACGTGAAGCC CGTGGTGGTG
    CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAATTGAGA TCCAAAACCA GCTCCTCAGG
    GTCGCTGACC GCCTGGAAGC TAACCTGGAG CAGGTCCACT TGGCTCTGAA GGCACAGTGT
    TCCTCCGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC
    AGCCAGCCCC TGTGTGACAT CCACGAGATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG
    CTCCGGCTAC GCACCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCTCTGGC CTTGAAGCTT
    GGCCATAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT
    GCCCTGGCCG AGGAGCCCCA GAACAGCCTG CCGGACATCA TCATCTGGAT GCTGCAGGGT
    GACAAGCGTG TGGCCTACCA GCGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC
    CCCAGCTACT GCGGCAGGAA CTGTGGGAAG CTGCAGACCA TCTTTCTGAA ATATCCTATG
    GAAGGTATGC CTGGGGCCCG AATGCCCGTG CAGATTCGGA TCAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAATT CAATCAGTTT GCTGAGGGAA AGCTCTCTGT CTTTGCCGAA
    ACGTATGAGA ACCAGACAAA GCTGGCACTG GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGATGT CACAGGCAAG ATCAAACTAC CAAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGGCCTGGGC AGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GATGCTGACG CTGGTCATCT GAGCTTCGTG GAGGAGGTGT TCGAGAACCA GACCCGGCTC
    CCTGGGGGCC AGTGGATTTA CATGAGTGAT AACTACACAG ATGTGAATGG GGAGAAGGTA
    CTTCCGAAGG ATGATATTGA ATGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA
    GACCGAAAGC CAAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA
    CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AAGCACTGAA GAGGCACAGG
    CAGGCAGAGG CCGAGGGCGA GGGCTGGGAG TATGCCTCTC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCG
    CTGGAGAAGA CGGGACCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCATGGTG
    GATGACAAGA GCGAAGACTC CATGTCTGTT TCTACCCTGA GCTTTGGCGT TAACAGACCC
    ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGCTGCTA CCTGTACCAG
    GCCCGAGACC TGCCAGCCAT GGACAAGGAC TCCTTCTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCATC AAAGCCAGAA GACAGTGGTG GAGAAGAACA CCCTGAATCC CACCTGGGAT
    CAAACCCTCA TCTTCTATGA GATTGAGATC TTCGGCGAGC CAGCCAGTAT CGCCGAGCAT
    CCGCCCTGCA TCGTGGTGGA GCTCTATGAC CACGACACCT ATGGTGCTGA CGAGTTCATG
    GGTCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG
    ACCCGGGGCA GCCAGCCTGC CGGCGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG
    AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCATG AAACTTCAAG GATTTTGGAT
    GAGACGGAGG ACACAGATTT GCCTTACCCA CCACCTCAAA GGGAGGCCAA CATCTACATG
    GTTCCCCAGA ACATTAAGCC AGCTCTCCAA CGTACTGCCA TTGAGATCCT GGCCTGGGGC
    CTGCGGAACA TGAAGAGCTA TCAGATGGCC AGCATCTCTT CCCCCAGCCT CGTGGTGGAG
    TGTGGTGGGC AGACAGTGCA GTCCTGTGTC ATCAGAAACC TTCGGAAGAA CCCCAACTTT
    GATGTCTGTA CTCTCTTCAT GGAAGTGATG TTACCCAGAG AGGACCTCTA CTGTCCACCT
    ATTGTGGTCA AGGTCATTGA TAATCGCCAG TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT
    ACCATTCGCT CCCTGGAGAA CTTCCTGTGC GACCCATACT CAGCAGAGAG TCCATCCCCA
    CAGGGTGGCC CAGATGATGT GAGCTTACTC AGCCCCGGGG AAGATGTGCT GATTGACATC
    GATGACAAGG AGCCTCTCAT TCCTGTCCAG GAGGAGGAGT TCATTGACTG GTGGAGCAAA
    TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC
    ACCTTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CTTTTGGAGG CCTGTCCGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AGGACTCAGG AGGAGACAGA TGATCCATCT
    GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC
    CCCATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC
    CGTATTTATA TCGTCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT
    TGCACCCTGG AGCCTGTATT CGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG
    AAGGACCTGA AGATCACACT CTACGATTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG
    GAGACGGTCA TAGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC
    CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG
    CTCCTCCACC TCTTCTGCCA GCAGCACAGG ATCAAGGCCC CCGTGTACCG GACAGACCGA
    GTGACGTTTC AGGATAAGGA CTACACCATT GAGGAGATAG AGGCTGGCAG ACTCCCAAAC
    CCACACCTGG GCCCAGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAGCA ACAAGGCTTG
    GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT TATAGTCCTC TGCAGCCAGA TATCGAGCAG
    GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC
    TTCAACATCA CCCCACGGAA AGCTAGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA
    AAAGATGTGA TCTTGGATGA CCTCAGTCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG
    AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAGCAGAAGA CAGATGTGCA CTACCGCTCC
    CTGGGTGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCTGCT
    GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCCTTCTGGA GACTCGACAA GACAGAGAGC
    AAGATCCCAG CACGTGTGGT CTTCCAGATC TGGGACAATG ACAAATTCTC TTTTGATGAC
    TTTCTGGGCT CTCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT
    GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGA GAAGAAGATG
    TTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTTGCAG AAAGTGAACA TGAAGAGCGG
    CCTGCTGGCC AAGGTCGGGA TGAACCCAAC ATGAATCCGA AGCTAGAGGA TCCAAGGCGC
    CCCGATACTT CTTTCCTGTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCTTGTGG
    CGACGCTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG
    GGTGTCTTTG TCTACGCCTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTTCGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37308
    Clone ID Related Accession (Same CDS sequence) NM_001310152.1
    Accession Version NM_001310152.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Mus musculus(house mouse)
    Product dysferlin isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN716462.1 and AK131144.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to exon 1. It encodes isoform 3, which lacks an internal segment and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGT GGACGACAAC
    CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG
    TTCACGGGCT TCCCAGATCC TTATGCAGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCT
    TATCGAGGCA GGGTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAACACAG TGAGCAGAAG
    GTGGAAGACC TTCCTGCTGA TGATATCCTG CGTGTGGAGA AGTACCTGCG GAGGCGCAAA
    TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGACGCCATC
    CAGTTCGAGG TCAGCATTGG GAACTACGGG AACAAGTTCG ACACCACCTG CCTGCCCTTG
    GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGATGGAT GCCACTACTA TTACTTGCCT
    TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCCTCATACT GGGAAGACAT CAGCCATCGA
    ATTGAGATCC AAAACCAGCT CCTCAGGGTC GCTGACCGCC TGGAAGCTAA CCTGGAGCAG
    GTCCACTTGG CTCTGAAGGC ACAGTGTTCC TCCGAGGATG TGGACGCCCT GGTGGCTCAG
    TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA CGAGATACCT
    TCTGCCACCC ACCTTGATCA GTACCTGCTC CGGCTACGCA CCCGTCACCT GAGCCAGATC
    AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC CATAGTGAGC TCTCCACTGC CCTGGAGCAG
    GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG
    GACATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAGCG GGTGCCTGCC
    CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAAGCTG
    CAGACCATCT TTCTGAAATA TCCTATGGAA GGTATGCCTG GGGCCCGAAT GCCCGTGCAG
    ATTCGGATCA AGCTCTGGTT CGGGCTCTCT GTGGATGAGA AGGAATTCAA TCAGTTTGCT
    GAGGGAAAGC TCTCTGTCTT TGCCGAAACG TATGAGAACC AGACAAAGCT GGCACTGGTC
    GGGAACTGGG GCACAACGGG CCTCACCTAC CCCAAGTTTT CTGATGTCAC AGGCAAGATC
    AAACTACCAA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGATTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAT GCTGACGCTG GTCATCTGAG CTTCGTGGAG
    GAGGTGTTCG AGAACCAGAC CCGGCTCCCT GGGGGCCAGT GGATTTACAT GAGTGATAAC
    TACACAGATG TGAATGGGGA GAAGGTACTT CCGAAGGATG ATATTGAATG CCCGCTGGGC
    TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC
    TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCAA AACACTGGGT CCCTGTTGAG
    AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC
    CAGATGGAAG CACTGAAGAG GCACAGGCAG GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT
    GCCTCTCTCT TCGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG GAGAAGACGG GACCTGCAGC TGTGTTTGCC
    CTCGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGACTCCAT GTCTGTTTCT
    ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC
    TACCATCTGC GCTGCTACCT GTACCAGGCC CGAGACCTGC CAGCCATGGA CAAGGACTCC
    TTCTCTGATC CCTACGCCAT CGTCTCCTTC CTGCATCAAA GCCAGAAGAC AGTGGTGGAG
    AAGAACACCC TGAATCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT TGAGATCTTC
    GGCGAGCCAG CCAGTATCGC CGAGCATCCG CCCTGCATCG TGGTGGAGCT CTATGACCAC
    GACACCTATG GTGCTGACGA GTTCATGGGT CGCTGTATTT GTCAGCCAAG CCTGGAACGG
    ATGCCCCGCC TGGCCTGGTT CCCACTGACC CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG
    GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG
    ATGCATGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGATTTGCC TTACCCACCA
    CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TTAAGCCAGC TCTCCAACGT
    ACTGCCATTG AGATCCTGGC CTGGGGCCTG CGGAACATGA AGAGCTATCA GATGGCCAGC
    ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC
    AGAAACCTTC GGAAGAACCC CAACTTTGAT GTCTGTACTC TCTTCATGGA AGTGATGTTA
    CCCAGAGAGG ACCTCTACTG TCCACCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT
    GGCCGCCGGC CAGTTGTGGG TCAGTGTACC ATTCGCTCCC TGGAGAACTT CCTGTGCGAC
    CCATACTCAG CAGAGAGTCC ATCCCCACAG GGTGGCCCAG ATGATGTGAG CTTACTCAGC
    CCCGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CTCTCATTCC TGTCCAGGAG
    GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT
    GGTTCCTACC TGGAGAAGGA TTTTGACACC TTGAAGGTCT ATGACACACA GCTGGAGAAT
    GTGGAGGCTT TTGGAGGCCT GTCCGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAGG
    ACTCAGGAGG AGACAGATGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT
    TATCCCCTCC CAGAAGATCC AGCCATCCCC ATGCCCCCGA GACAATTCCA TCAGCTGGCT
    GCTCAGGGTC CCCAGGAATG CTTGGTCCGT ATTTATATCG TCCGAGCATT TGGCTTACAG
    CCCAAGGACC CCAATGGCAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATCCCTTGC ACCCTGGAGC CTGTATTCGG AAAGATGTTT
    GAACTCACCT GCACTCTACC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGATTATGAC
    CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACGGTCATAG ACCTGGAGAA CAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG
    TGGAGAGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGATC
    AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGACTA CACCATTGAG
    GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CAGTGGAGGA ACGCTTAGCC
    CTGCATGTCC TTCAGCAACA AGGCTTGGTT CCTGAGCATG TGGAGTCACG GCCTCTTTAT
    AGTCCTCTGC AGCCAGATAT CGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA
    AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TAGAAGGTTT
    TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGTCTCACG
    GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAG
    CAGAAGACAG ATGTGCACTA CCGCTCCCTG GGTGGGGAGG GCAATTTCAA CTGGAGGTTC
    GTGTTTCCCT TCGACTATCT GCCTGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCC
    TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATCTGG
    GACAATGACA AATTCTCTTT TGATGACTTT CTGGGCTCTC TGCAGCTGGA TCTCAACCGA
    ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC
    CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT
    GTGACAGAGG AGGGCGAGAA GAAGATGTTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT
    GTTGCAGAAA GTGAACATGA AGAGCGGCCT GCTGGCCAAG GTCGGGATGA ACCCAACATG
    AATCCGAAGC TAGAGGATCC AAGGCGCCCC GATACTTCTT TCCTGTGGTT CACCTCCCCG
    TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGCTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001297081.1
    CDS133..6342
    Translation

    Target ORF information:

    RefSeq Version NM_001310152.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001310152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGT GGACGACAAC
    CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG
    TTCACGGGCT TCCCAGATCC TTATGCAGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCT
    TATCGAGGCA GGGTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAACACAG TGAGCAGAAG
    GTGGAAGACC TTCCTGCTGA TGATATCCTG CGTGTGGAGA AGTACCTGCG GAGGCGCAAA
    TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGACGCCATC
    CAGTTCGAGG TCAGCATTGG GAACTACGGG AACAAGTTCG ACACCACCTG CCTGCCCTTG
    GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGATGGAT GCCACTACTA TTACTTGCCT
    TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCCTCATACT GGGAAGACAT CAGCCATCGA
    ATTGAGATCC AAAACCAGCT CCTCAGGGTC GCTGACCGCC TGGAAGCTAA CCTGGAGCAG
    GTCCACTTGG CTCTGAAGGC ACAGTGTTCC TCCGAGGATG TGGACGCCCT GGTGGCTCAG
    TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA CGAGATACCT
    TCTGCCACCC ACCTTGATCA GTACCTGCTC CGGCTACGCA CCCGTCACCT GAGCCAGATC
    AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC CATAGTGAGC TCTCCACTGC CCTGGAGCAG
    GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG
    GACATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAGCG GGTGCCTGCC
    CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAAGCTG
    CAGACCATCT TTCTGAAATA TCCTATGGAA GGTATGCCTG GGGCCCGAAT GCCCGTGCAG
    ATTCGGATCA AGCTCTGGTT CGGGCTCTCT GTGGATGAGA AGGAATTCAA TCAGTTTGCT
    GAGGGAAAGC TCTCTGTCTT TGCCGAAACG TATGAGAACC AGACAAAGCT GGCACTGGTC
    GGGAACTGGG GCACAACGGG CCTCACCTAC CCCAAGTTTT CTGATGTCAC AGGCAAGATC
    AAACTACCAA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGATTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAT GCTGACGCTG GTCATCTGAG CTTCGTGGAG
    GAGGTGTTCG AGAACCAGAC CCGGCTCCCT GGGGGCCAGT GGATTTACAT GAGTGATAAC
    TACACAGATG TGAATGGGGA GAAGGTACTT CCGAAGGATG ATATTGAATG CCCGCTGGGC
    TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC
    TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCAA AACACTGGGT CCCTGTTGAG
    AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC
    CAGATGGAAG CACTGAAGAG GCACAGGCAG GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT
    GCCTCTCTCT TCGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG GAGAAGACGG GACCTGCAGC TGTGTTTGCC
    CTCGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGACTCCAT GTCTGTTTCT
    ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC
    TACCATCTGC GCTGCTACCT GTACCAGGCC CGAGACCTGC CAGCCATGGA CAAGGACTCC
    TTCTCTGATC CCTACGCCAT CGTCTCCTTC CTGCATCAAA GCCAGAAGAC AGTGGTGGAG
    AAGAACACCC TGAATCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT TGAGATCTTC
    GGCGAGCCAG CCAGTATCGC CGAGCATCCG CCCTGCATCG TGGTGGAGCT CTATGACCAC
    GACACCTATG GTGCTGACGA GTTCATGGGT CGCTGTATTT GTCAGCCAAG CCTGGAACGG
    ATGCCCCGCC TGGCCTGGTT CCCACTGACC CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG
    GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG
    ATGCATGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGATTTGCC TTACCCACCA
    CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TTAAGCCAGC TCTCCAACGT
    ACTGCCATTG AGATCCTGGC CTGGGGCCTG CGGAACATGA AGAGCTATCA GATGGCCAGC
    ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC
    AGAAACCTTC GGAAGAACCC CAACTTTGAT GTCTGTACTC TCTTCATGGA AGTGATGTTA
    CCCAGAGAGG ACCTCTACTG TCCACCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT
    GGCCGCCGGC CAGTTGTGGG TCAGTGTACC ATTCGCTCCC TGGAGAACTT CCTGTGCGAC
    CCATACTCAG CAGAGAGTCC ATCCCCACAG GGTGGCCCAG ATGATGTGAG CTTACTCAGC
    CCCGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CTCTCATTCC TGTCCAGGAG
    GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT
    GGTTCCTACC TGGAGAAGGA TTTTGACACC TTGAAGGTCT ATGACACACA GCTGGAGAAT
    GTGGAGGCTT TTGGAGGCCT GTCCGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAGG
    ACTCAGGAGG AGACAGATGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT
    TATCCCCTCC CAGAAGATCC AGCCATCCCC ATGCCCCCGA GACAATTCCA TCAGCTGGCT
    GCTCAGGGTC CCCAGGAATG CTTGGTCCGT ATTTATATCG TCCGAGCATT TGGCTTACAG
    CCCAAGGACC CCAATGGCAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATCCCTTGC ACCCTGGAGC CTGTATTCGG AAAGATGTTT
    GAACTCACCT GCACTCTACC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGATTATGAC
    CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACGGTCATAG ACCTGGAGAA CAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG
    TGGAGAGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGATC
    AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGACTA CACCATTGAG
    GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CAGTGGAGGA ACGCTTAGCC
    CTGCATGTCC TTCAGCAACA AGGCTTGGTT CCTGAGCATG TGGAGTCACG GCCTCTTTAT
    AGTCCTCTGC AGCCAGATAT CGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA
    AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TAGAAGGTTT
    TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGTCTCACG
    GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAG
    CAGAAGACAG ATGTGCACTA CCGCTCCCTG GGTGGGGAGG GCAATTTCAA CTGGAGGTTC
    GTGTTTCCCT TCGACTATCT GCCTGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCC
    TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATCTGG
    GACAATGACA AATTCTCTTT TGATGACTTT CTGGGCTCTC TGCAGCTGGA TCTCAACCGA
    ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC
    CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT
    GTGACAGAGG AGGGCGAGAA GAAGATGTTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT
    GTTGCAGAAA GTGAACATGA AGAGCGGCCT GCTGGCCAAG GTCGGGATGA ACCCAACATG
    AATCCGAAGC TAGAGGATCC AAGGCGCCCC GATACTTCTT TCCTGTGGTT CACCTCCCCG
    TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGCTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37291
    Clone ID Related Accession (Same CDS sequence) XM_006506163.2
    Accession Version XM_006506163.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATAAGATCC TCCTGGAGGA GGAGTTCATT GACTGGTGGA GCAAATTCTT TGCTTCTGTC
    GGGGAGAGGG AAAAGTGTGG TTCCTACCTG GAGAAGGATT TTGACACCTT GAAGGTCTAT
    GACACACAGC TGGAGAATGT GGAGGCTTTT GGAGGCCTGT CCGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAGGAC TCAGGAGGAG ACAGATGATC CATCTGTCAT CGGAGAATTT
    AAGGGTCTCT TCAAAATTTA TCCCCTCCCA GAAGATCCAG CCATCCCCAT GCCCCCGAGA
    CAATTCCATC AGCTGGCTGC TCAGGGTCCC CAGGAATGCT TGGTCCGTAT TTATATCGTC
    CGAGCATTTG GCTTACAGCC CAAGGACCCC AATGGCAAGT GTGACCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCTTGCAC CCTGGAGCCT
    GTATTCGGAA AGATGTTTGA ACTCACCTGC ACTCTACCTC TGGAGAAGGA CCTGAAGATC
    ACACTCTACG ATTATGACCT GCTCTCCAAG GATGAGAAGA TTGGGGAGAC GGTCATAGAC
    CTGGAGAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GGCTCCCACA GACCTACTGT
    GTCTCTGGAC CAAATCAGTG GAGAGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC
    TGCCAGCAGC ACAGGATCAA GGCCCCCGTG TACCGGACAG ACCGAGTGAC GTTTCAGGAT
    AAGGACTACA CCATTGAGGA GATAGAGGCT GGCAGACTCC CAAACCCACA CCTGGGCCCA
    GTGGAGGAAC GCTTAGCCCT GCATGTCCTT CAGCAACAAG GCTTGGTTCC TGAGCATGTG
    GAGTCACGGC CTCTTTATAG TCCTCTGCAG CCAGATATCG AGCAGGGGAA GCTACAGATG
    TGGATTGACA TATTTCCAAA GGTGCTGGGC CGGCCTGGAC CTCCCTTCAA CATCACCCCA
    CGGAAAGCTA GAAGGTTTTT TCTGCGTTGT ATTATTTGGA ACACAAAAGA TGTGATCTTG
    GATGACCTCA GTCTCACGGG GGAGAAGATG AGTGACATCT ATGTGAAAGG CTGGATGGTG
    GGATTCGAAG AACACAAGCA GAAGACAGAT GTGCACTACC GCTCCCTGGG TGGGGAGGGC
    AATTTCAACT GGAGGTTCGT GTTTCCCTTC GACTATCTGC CTGCTGAGCA AGTCTGTGCT
    GTTGCCAAGA AGGATGCCTT CTGGAGACTC GACAAGACAG AGAGCAAGAT CCCAGCACGT
    GTGGTCTTCC AGATCTGGGA CAATGACAAA TTCTCTTTTG ATGACTTTCT GGGCTCTCTG
    CAGCTGGATC TCAACCGAAT GCCCAAGCCA GCCAAGACGG CTGAGAAGTG CTCCTTGGAC
    CAGCTGGATG ACACCTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAGACAGTG
    AAAGGATGGT GGCCTTGTGT GACAGAGGAG GGCGAGAAGA AGATGTTGGC GGGCAAGCTG
    GAAATGACCT TGGAGATTGT TGCAGAAAGT GAACATGAAG AGCGGCCTGC TGGCCAAGGT
    CGGGATGAAC CCAACATGAA TCCGAAGCTA GAGGATCCAA GGCGCCCCGA TACTTCTTTC
    CTGTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCT TGTGGCGACG CTTCCGGTGT
    GCCATCATCC TCTTCATCAT CCTCTTCATC CTCCTGCTCT TCCTGGGTGT CTTTGTCTAC
    GCCTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506226.1
    CDS256..6723
    Translation

    Target ORF information:

    RefSeq Version XM_006506163.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506163.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATAAGATCC TCCTGGAGGA GGAGTTCATT GACTGGTGGA GCAAATTCTT TGCTTCTGTC
    GGGGAGAGGG AAAAGTGTGG TTCCTACCTG GAGAAGGATT TTGACACCTT GAAGGTCTAT
    GACACACAGC TGGAGAATGT GGAGGCTTTT GGAGGCCTGT CCGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAGGAC TCAGGAGGAG ACAGATGATC CATCTGTCAT CGGAGAATTT
    AAGGGTCTCT TCAAAATTTA TCCCCTCCCA GAAGATCCAG CCATCCCCAT GCCCCCGAGA
    CAATTCCATC AGCTGGCTGC TCAGGGTCCC CAGGAATGCT TGGTCCGTAT TTATATCGTC
    CGAGCATTTG GCTTACAGCC CAAGGACCCC AATGGCAAGT GTGACCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCTTGCAC CCTGGAGCCT
    GTATTCGGAA AGATGTTTGA ACTCACCTGC ACTCTACCTC TGGAGAAGGA CCTGAAGATC
    ACACTCTACG ATTATGACCT GCTCTCCAAG GATGAGAAGA TTGGGGAGAC GGTCATAGAC
    CTGGAGAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GGCTCCCACA GACCTACTGT
    GTCTCTGGAC CAAATCAGTG GAGAGATCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC
    TGCCAGCAGC ACAGGATCAA GGCCCCCGTG TACCGGACAG ACCGAGTGAC GTTTCAGGAT
    AAGGACTACA CCATTGAGGA GATAGAGGCT GGCAGACTCC CAAACCCACA CCTGGGCCCA
    GTGGAGGAAC GCTTAGCCCT GCATGTCCTT CAGCAACAAG GCTTGGTTCC TGAGCATGTG
    GAGTCACGGC CTCTTTATAG TCCTCTGCAG CCAGATATCG AGCAGGGGAA GCTACAGATG
    TGGATTGACA TATTTCCAAA GGTGCTGGGC CGGCCTGGAC CTCCCTTCAA CATCACCCCA
    CGGAAAGCTA GAAGGTTTTT TCTGCGTTGT ATTATTTGGA ACACAAAAGA TGTGATCTTG
    GATGACCTCA GTCTCACGGG GGAGAAGATG AGTGACATCT ATGTGAAAGG CTGGATGGTG
    GGATTCGAAG AACACAAGCA GAAGACAGAT GTGCACTACC GCTCCCTGGG TGGGGAGGGC
    AATTTCAACT GGAGGTTCGT GTTTCCCTTC GACTATCTGC CTGCTGAGCA AGTCTGTGCT
    GTTGCCAAGA AGGATGCCTT CTGGAGACTC GACAAGACAG AGAGCAAGAT CCCAGCACGT
    GTGGTCTTCC AGATCTGGGA CAATGACAAA TTCTCTTTTG ATGACTTTCT GGGCTCTCTG
    CAGCTGGATC TCAACCGAAT GCCCAAGCCA GCCAAGACGG CTGAGAAGTG CTCCTTGGAC
    CAGCTGGATG ACACCTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAGACAGTG
    AAAGGATGGT GGCCTTGTGT GACAGAGGAG GGCGAGAAGA AGATGTTGGC GGGCAAGCTG
    GAAATGACCT TGGAGATTGT TGCAGAAAGT GAACATGAAG AGCGGCCTGC TGGCCAAGGT
    CGGGATGAAC CCAACATGAA TCCGAAGCTA GAGGATCCAA GGCGCCCCGA TACTTCTTTC
    CTGTGGTTCA CCTCCCCGTA CAAGACCATG AAGTTCATCT TGTGGCGACG CTTCCGGTGT
    GCCATCATCC TCTTCATCAT CCTCTTCATC CTCCTGCTCT TCCTGGGTGT CTTTGTCTAC
    GCCTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37292
    Clone ID Related Accession (Same CDS sequence) XM_006506164.3 , XM_006506164.4
    Accession Version XM_006506164.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506164.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCACA AGCCACTGAC
    CTGGACGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC
    AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCAG AGCTCAACAC AGGAAAGGGA
    GAAGGTGTGG CTTATCGAGG CAGGGTTCTG TTGTCCCTGG AGACCAAACT GGTGGAACAC
    AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT GATGATATCC TGCGTGTGGA GAAGTACCTG
    CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG
    GATGACGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GGAACAAGTT CGACACCACC
    TGCCTGCCCT TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC
    TATTACTTGC CTTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCCTCATA CTGGGAAGAC
    ATCAGCCATC GAATTGAGAT CCAAAACCAG CTCCTCAGGG TCGCTGACCG CCTGGAAGCT
    AACCTGGAGC AGGTCCACTT GGCTCTGAAG GCACAGTGTT CCTCCGAGGA TGTGGACGCC
    CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC
    CACGAGATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGC TCCGGCTACG CACCCGTCAC
    CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC TTGAAGCTTG GCCATAGTGA GCTCTCCACT
    GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCCGA GGAGCCCCAG
    AACAGCCTGC CGGACATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAG
    CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC
    TGTGGGAAGC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGTATGCC TGGGGCCCGA
    ATGCCCGTGC AGATTCGGAT CAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAATTC
    AATCAGTTTG CTGAGGGAAA GCTCTCTGTC TTTGCCGAAA CGTATGAGAA CCAGACAAAG
    CTGGCACTGG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ACAGGCAAGA TCAAACTACC AAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA
    GGAGATTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ATGCTGACGC TGGTCATCTG
    AGCTTCGTGG AGGAGGTGTT CGAGAACCAG ACCCGGCTCC CTGGGGGCCA GTGGATTTAC
    ATGAGTGATA ACTACACAGA TGTGAATGGG GAGAAGGTAC TTCCGAAGGA TGATATTGAA
    TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT
    GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC AAAACACTGG
    GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG
    AGGGACCTCA GCCAGATGGA AGCACTGAAG AGGCACAGGC AGGCAGAGGC CGAGGGCGAG
    GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGACCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGACTCC
    ATGTCTGTTT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC
    TATGGGAACC GCTACCATCT GCGCTGCTAC CTGTACCAGG CCCGAGACCT GCCAGCCATG
    GACAAGGACT CCTTCTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCATCA AAGCCAGAAG
    ACAGTGGTGG AGAAGAACAC CCTGAATCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG
    ATTGAGATCT TCGGCGAGCC AGCCAGTATC GCCGAGCATC CGCCCTGCAT CGTGGTGGAG
    CTCTATGACC ACGACACCTA TGGTGCTGAC GAGTTCATGG GTCGCTGTAT TTGTCAGCCA
    AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCCGGGGCAG CCAGCCTGCC
    GGCGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC
    CCTGGCTTTG AGATGCATGA AACTTCAAGG ATTTTGGATG AGCTATGCAC GCCTGCCTTC
    TTCCTGCAGG GCTTCCTTCG GACGAGCACG ACGGAGGACA CAGATTTGCC TTACCCACCA
    CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TTAAGCCAGC TCTCCAACGT
    ACTGCCATTG AGATCCTGGC CTGGGGCCTG CGGAACATGA AGAGCTATCA GATGGCCAGC
    ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC
    AGAAACCTTC GGAAGAACCC CAACTTTGAT GTCTGTACTC TCTTCATGGA AGTGATGTTA
    CCCAGAGAGG ACCTCTACTG TCCACCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT
    GGCCGCCGGC CAGTTGTGGG TCAGTGTACC ATTCGCTCCC TGGAGAACTT CCTGTGCGAC
    CCATACTCAG CAGAGAGTCC ATCCCCACAG GGTGGCCCAG ATGATGTGAG CTTACTCAGC
    CCCGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CTCTCATTCC TGTCCAGCTT
    ACAGATGGGC TGTCAAGCTT GGGCCCCACT AACCTGACGC CTTCTCCATC CAGTCCTCAT
    AAGATCCTCC TGGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTGTCGGG
    GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCTTGAA GGTCTATGAC
    ACACAGCTGG AGAATGTGGA GGCTTTTGGA GGCCTGTCCG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAGGACTCA GGAGGAGACA GATGATCCAT CTGTCATCGG AGAATTTAAG
    GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCCATGCC CCCGAGACAA
    TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGTATTTA TATCGTCCGA
    GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGCACCCT GGAGCCTGTA
    TTCGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACCT GAAGATCACA
    CTCTACGATT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACGGT CATAGACCTG
    GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGGC TCCCACAGAC CTACTGTGTC
    TCTGGACCAA ATCAGTGGAG AGATCAGCTC CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC
    CAGCAGCACA GGATCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG
    GACTACACCA TTGAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCAGTG
    GAGGAACGCT TAGCCCTGCA TGTCCTTCAG CAACAAGGCT TGGTTCCTGA GCATGTGGAG
    TCACGGCCTC TTTATAGTCC TCTGCAGCCA GATATCGAGC AGGGGAAGCT ACAGATGTGG
    ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG
    AAAGCTAGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT
    GACCTCAGTC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA
    TTCGAAGAAC ACAAGCAGAA GACAGATGTG CACTACCGCT CCCTGGGTGG GGAGGGCAAT
    TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCTG CTGAGCAAGT CTGTGCTGTT
    GCCAAGAAGG ATGCCTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG
    GTCTTCCAGA TCTGGGACAA TGACAAATTC TCTTTTGATG ACTTTCTGGG CTCTCTGCAG
    CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA
    GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA
    ATGACCTTGG AGATTGTTGC AGAAAGTGAA CATGAAGAGC GGCCTGCTGG CCAAGGTCGG
    GATGAACCCA ACATGAATCC GAAGCTAGAG GATCCAAGGC GCCCCGATAC TTCTTTCCTG
    TGGTTCACCT CCCCGTACAA GACCATGAAG TTCATCTTGT GGCGACGCTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506227.1
    CDS602..7066
    Translation

    Target ORF information:

    RefSeq Version XM_006506164.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506164.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCACA AGCCACTGAC
    CTGGACGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC
    AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCAG AGCTCAACAC AGGAAAGGGA
    GAAGGTGTGG CTTATCGAGG CAGGGTTCTG TTGTCCCTGG AGACCAAACT GGTGGAACAC
    AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT GATGATATCC TGCGTGTGGA GAAGTACCTG
    CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG
    GATGACGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GGAACAAGTT CGACACCACC
    TGCCTGCCCT TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC
    TATTACTTGC CTTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCCTCATA CTGGGAAGAC
    ATCAGCCATC GAATTGAGAT CCAAAACCAG CTCCTCAGGG TCGCTGACCG CCTGGAAGCT
    AACCTGGAGC AGGTCCACTT GGCTCTGAAG GCACAGTGTT CCTCCGAGGA TGTGGACGCC
    CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC
    CACGAGATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGC TCCGGCTACG CACCCGTCAC
    CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC TTGAAGCTTG GCCATAGTGA GCTCTCCACT
    GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCCGA GGAGCCCCAG
    AACAGCCTGC CGGACATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAG
    CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC
    TGTGGGAAGC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGTATGCC TGGGGCCCGA
    ATGCCCGTGC AGATTCGGAT CAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAATTC
    AATCAGTTTG CTGAGGGAAA GCTCTCTGTC TTTGCCGAAA CGTATGAGAA CCAGACAAAG
    CTGGCACTGG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ACAGGCAAGA TCAAACTACC AAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA
    GGAGATTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ATGCTGACGC TGGTCATCTG
    AGCTTCGTGG AGGAGGTGTT CGAGAACCAG ACCCGGCTCC CTGGGGGCCA GTGGATTTAC
    ATGAGTGATA ACTACACAGA TGTGAATGGG GAGAAGGTAC TTCCGAAGGA TGATATTGAA
    TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT
    GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC AAAACACTGG
    GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG
    AGGGACCTCA GCCAGATGGA AGCACTGAAG AGGCACAGGC AGGCAGAGGC CGAGGGCGAG
    GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGACCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGACTCC
    ATGTCTGTTT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC
    TATGGGAACC GCTACCATCT GCGCTGCTAC CTGTACCAGG CCCGAGACCT GCCAGCCATG
    GACAAGGACT CCTTCTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCATCA AAGCCAGAAG
    ACAGTGGTGG AGAAGAACAC CCTGAATCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG
    ATTGAGATCT TCGGCGAGCC AGCCAGTATC GCCGAGCATC CGCCCTGCAT CGTGGTGGAG
    CTCTATGACC ACGACACCTA TGGTGCTGAC GAGTTCATGG GTCGCTGTAT TTGTCAGCCA
    AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCCGGGGCAG CCAGCCTGCC
    GGCGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC
    CCTGGCTTTG AGATGCATGA AACTTCAAGG ATTTTGGATG AGCTATGCAC GCCTGCCTTC
    TTCCTGCAGG GCTTCCTTCG GACGAGCACG ACGGAGGACA CAGATTTGCC TTACCCACCA
    CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TTAAGCCAGC TCTCCAACGT
    ACTGCCATTG AGATCCTGGC CTGGGGCCTG CGGAACATGA AGAGCTATCA GATGGCCAGC
    ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC
    AGAAACCTTC GGAAGAACCC CAACTTTGAT GTCTGTACTC TCTTCATGGA AGTGATGTTA
    CCCAGAGAGG ACCTCTACTG TCCACCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT
    GGCCGCCGGC CAGTTGTGGG TCAGTGTACC ATTCGCTCCC TGGAGAACTT CCTGTGCGAC
    CCATACTCAG CAGAGAGTCC ATCCCCACAG GGTGGCCCAG ATGATGTGAG CTTACTCAGC
    CCCGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CTCTCATTCC TGTCCAGCTT
    ACAGATGGGC TGTCAAGCTT GGGCCCCACT AACCTGACGC CTTCTCCATC CAGTCCTCAT
    AAGATCCTCC TGGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTGTCGGG
    GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCTTGAA GGTCTATGAC
    ACACAGCTGG AGAATGTGGA GGCTTTTGGA GGCCTGTCCG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAGGACTCA GGAGGAGACA GATGATCCAT CTGTCATCGG AGAATTTAAG
    GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCCATGCC CCCGAGACAA
    TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGTATTTA TATCGTCCGA
    GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGCACCCT GGAGCCTGTA
    TTCGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACCT GAAGATCACA
    CTCTACGATT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACGGT CATAGACCTG
    GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGGC TCCCACAGAC CTACTGTGTC
    TCTGGACCAA ATCAGTGGAG AGATCAGCTC CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC
    CAGCAGCACA GGATCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG
    GACTACACCA TTGAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCAGTG
    GAGGAACGCT TAGCCCTGCA TGTCCTTCAG CAACAAGGCT TGGTTCCTGA GCATGTGGAG
    TCACGGCCTC TTTATAGTCC TCTGCAGCCA GATATCGAGC AGGGGAAGCT ACAGATGTGG
    ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG
    AAAGCTAGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT
    GACCTCAGTC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA
    TTCGAAGAAC ACAAGCAGAA GACAGATGTG CACTACCGCT CCCTGGGTGG GGAGGGCAAT
    TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCTG CTGAGCAAGT CTGTGCTGTT
    GCCAAGAAGG ATGCCTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG
    GTCTTCCAGA TCTGGGACAA TGACAAATTC TCTTTTGATG ACTTTCTGGG CTCTCTGCAG
    CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA
    GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA
    ATGACCTTGG AGATTGTTGC AGAAAGTGAA CATGAAGAGC GGCCTGCTGG CCAAGGTCGG
    GATGAACCCA ACATGAATCC GAAGCTAGAG GATCCAAGGC GCCCCGATAC TTCTTTCCTG
    TGGTTCACCT CCCCGTACAA GACCATGAAG TTCATCTTGT GGCGACGCTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37293
    Clone ID Related Accession (Same CDS sequence) XM_006506165.2
    Accession Version XM_006506165.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATGAGGAGG AGTTCATTGA CTGGTGGAGC AAATTCTTTG CTTCTGTCGG GGAGAGGGAA
    AAGTGTGGTT CCTACCTGGA GAAGGATTTT GACACCTTGA AGGTCTATGA CACACAGCTG
    GAGAATGTGG AGGCTTTTGG AGGCCTGTCC GACTTTTGTA ACACCTTCAA GCTCTACCGG
    GGCAGGACTC AGGAGGAGAC AGATGATCCA TCTGTCATCG GAGAATTTAA GGGTCTCTTC
    AAAATTTATC CCCTCCCAGA AGATCCAGCC ATCCCCATGC CCCCGAGACA ATTCCATCAG
    CTGGCTGCTC AGGGTCCCCA GGAATGCTTG GTCCGTATTT ATATCGTCCG AGCATTTGGC
    TTACAGCCCA AGGACCCCAA TGGCAAGTGT GACCCTTACA TCAAGATCTC CATAGGGAAG
    AAATCAGTGA GTGACCAGGA TAACTACATC CCTTGCACCC TGGAGCCTGT ATTCGGAAAG
    ATGTTTGAAC TCACCTGCAC TCTACCTCTG GAGAAGGACC TGAAGATCAC ACTCTACGAT
    TATGACCTGC TCTCCAAGGA TGAGAAGATT GGGGAGACGG TCATAGACCT GGAGAACAGG
    CTGCTGTCCA AGTTTGGGGC TCGCTGTGGG CTCCCACAGA CCTACTGTGT CTCTGGACCA
    AATCAGTGGA GAGATCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC
    AGGATCAAGG CCCCCGTGTA CCGGACAGAC CGAGTGACGT TTCAGGATAA GGACTACACC
    ATTGAGGAGA TAGAGGCTGG CAGACTCCCA AACCCACACC TGGGCCCAGT GGAGGAACGC
    TTAGCCCTGC ATGTCCTTCA GCAACAAGGC TTGGTTCCTG AGCATGTGGA GTCACGGCCT
    CTTTATAGTC CTCTGCAGCC AGATATCGAG CAGGGGAAGC TACAGATGTG GATTGACATA
    TTTCCAAAGG TGCTGGGCCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAAAGCTAGA
    AGGTTTTTTC TGCGTTGTAT TATTTGGAAC ACAAAAGATG TGATCTTGGA TGACCTCAGT
    CTCACGGGGG AGAAGATGAG TGACATCTAT GTGAAAGGCT GGATGGTGGG ATTCGAAGAA
    CACAAGCAGA AGACAGATGT GCACTACCGC TCCCTGGGTG GGGAGGGCAA TTTCAACTGG
    AGGTTCGTGT TTCCCTTCGA CTATCTGCCT GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG
    GATGCCTTCT GGAGACTCGA CAAGACAGAG AGCAAGATCC CAGCACGTGT GGTCTTCCAG
    ATCTGGGACA ATGACAAATT CTCTTTTGAT GACTTTCTGG GCTCTCTGCA GCTGGATCTC
    AACCGAATGC CCAAGCCAGC CAAGACGGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC
    ACCTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AGACAGTGAA AGGATGGTGG
    CCTTGTGTGA CAGAGGAGGG CGAGAAGAAG ATGTTGGCGG GCAAGCTGGA AATGACCTTG
    GAGATTGTTG CAGAAAGTGA ACATGAAGAG CGGCCTGCTG GCCAAGGTCG GGATGAACCC
    AACATGAATC CGAAGCTAGA GGATCCAAGG CGCCCCGATA CTTCTTTCCT GTGGTTCACC
    TCCCCGTACA AGACCATGAA GTTCATCTTG TGGCGACGCT TCCGGTGTGC CATCATCCTC
    TTCATCATCC TCTTCATCCT CCTGCTCTTC CTGGGTGTCT TTGTCTACGC CTTCCCGAAC
    TACGCTGCCA TGAAGCTGGT GAAGCCCTTT CGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506228.1
    CDS256..6711
    Translation

    Target ORF information:

    RefSeq Version XM_006506165.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506165.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    CTTACAGATG GGCTGTCAAG CTTGGGCCCC ACTAACCTGA CGCCTTCTCC ATCCAGTCCT
    CATGAGGAGG AGTTCATTGA CTGGTGGAGC AAATTCTTTG CTTCTGTCGG GGAGAGGGAA
    AAGTGTGGTT CCTACCTGGA GAAGGATTTT GACACCTTGA AGGTCTATGA CACACAGCTG
    GAGAATGTGG AGGCTTTTGG AGGCCTGTCC GACTTTTGTA ACACCTTCAA GCTCTACCGG
    GGCAGGACTC AGGAGGAGAC AGATGATCCA TCTGTCATCG GAGAATTTAA GGGTCTCTTC
    AAAATTTATC CCCTCCCAGA AGATCCAGCC ATCCCCATGC CCCCGAGACA ATTCCATCAG
    CTGGCTGCTC AGGGTCCCCA GGAATGCTTG GTCCGTATTT ATATCGTCCG AGCATTTGGC
    TTACAGCCCA AGGACCCCAA TGGCAAGTGT GACCCTTACA TCAAGATCTC CATAGGGAAG
    AAATCAGTGA GTGACCAGGA TAACTACATC CCTTGCACCC TGGAGCCTGT ATTCGGAAAG
    ATGTTTGAAC TCACCTGCAC TCTACCTCTG GAGAAGGACC TGAAGATCAC ACTCTACGAT
    TATGACCTGC TCTCCAAGGA TGAGAAGATT GGGGAGACGG TCATAGACCT GGAGAACAGG
    CTGCTGTCCA AGTTTGGGGC TCGCTGTGGG CTCCCACAGA CCTACTGTGT CTCTGGACCA
    AATCAGTGGA GAGATCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC
    AGGATCAAGG CCCCCGTGTA CCGGACAGAC CGAGTGACGT TTCAGGATAA GGACTACACC
    ATTGAGGAGA TAGAGGCTGG CAGACTCCCA AACCCACACC TGGGCCCAGT GGAGGAACGC
    TTAGCCCTGC ATGTCCTTCA GCAACAAGGC TTGGTTCCTG AGCATGTGGA GTCACGGCCT
    CTTTATAGTC CTCTGCAGCC AGATATCGAG CAGGGGAAGC TACAGATGTG GATTGACATA
    TTTCCAAAGG TGCTGGGCCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAAAGCTAGA
    AGGTTTTTTC TGCGTTGTAT TATTTGGAAC ACAAAAGATG TGATCTTGGA TGACCTCAGT
    CTCACGGGGG AGAAGATGAG TGACATCTAT GTGAAAGGCT GGATGGTGGG ATTCGAAGAA
    CACAAGCAGA AGACAGATGT GCACTACCGC TCCCTGGGTG GGGAGGGCAA TTTCAACTGG
    AGGTTCGTGT TTCCCTTCGA CTATCTGCCT GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG
    GATGCCTTCT GGAGACTCGA CAAGACAGAG AGCAAGATCC CAGCACGTGT GGTCTTCCAG
    ATCTGGGACA ATGACAAATT CTCTTTTGAT GACTTTCTGG GCTCTCTGCA GCTGGATCTC
    AACCGAATGC CCAAGCCAGC CAAGACGGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC
    ACCTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AGACAGTGAA AGGATGGTGG
    CCTTGTGTGA CAGAGGAGGG CGAGAAGAAG ATGTTGGCGG GCAAGCTGGA AATGACCTTG
    GAGATTGTTG CAGAAAGTGA ACATGAAGAG CGGCCTGCTG GCCAAGGTCG GGATGAACCC
    AACATGAATC CGAAGCTAGA GGATCCAAGG CGCCCCGATA CTTCTTTCCT GTGGTTCACC
    TCCCCGTACA AGACCATGAA GTTCATCTTG TGGCGACGCT TCCGGTGTGC CATCATCCTC
    TTCATCATCC TCTTCATCCT CCTGCTCTTC CTGGGTGTCT TTGTCTACGC CTTCCCGAAC
    TACGCTGCCA TGAAGCTGGT GAAGCCCTTT CGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37294
    Clone ID Related Accession (Same CDS sequence) XM_006506166.2
    Accession Version XM_006506166.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGTGGACGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC
    TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCA
    GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCTTATCGAG GCAGGGTTCT GTTGTCCCTG
    GAGACCAAAC TGGTGGAACA CAGTGAGCAG AAGGTGGAAG ACCTTCCTGC TGATGATATC
    CTGCGTGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA
    GCCACAATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC
    GGGAACAAGT TCGACACCAC CTGCCTGCCC TTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGATG GATGCCACTA CTATTACTTG CCTTGGGGCA ACGTGAAGCC CGTGGTGGTG
    CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAATTGAGA TCCAAAACCA GCTCCTCAGG
    GTCGCTGACC GCCTGGAAGC TAACCTGGAG CAGGTCCACT TGGCTCTGAA GGCACAGTGT
    TCCTCCGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC
    AGCCAGCCCC TGTGTGACAT CCACGAGATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG
    CTCCGGCTAC GCACCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCTCTGGC CTTGAAGCTT
    GGCCATAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT
    GCCCTGGCCG AGGAGCCCCA GAACAGCCTG CCGGACATCA TCATCTGGAT GCTGCAGGGT
    GACAAGCGTG TGGCCTACCA GCGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC
    CCCAGCTACT GCGGCAGGAA CTGTGGGAAG CTGCAGACCA TCTTTCTGAA ATATCCTATG
    GAAGGTATGC CTGGGGCCCG AATGCCCGTG CAGATTCGGA TCAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAATT CAATCAGTTT GCTGAGGGAA AGCTCTCTGT CTTTGCCGAA
    ACGTATGAGA ACCAGACAAA GCTGGCACTG GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGATGT CACAGGCAAG ATCAAACTAC CAAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGGCCTGGGC AGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GATGCTGACG CTGGTCATCT GAGCTTCGTG GAGGAGGTGT TCGAGAACCA GACCCGGCTC
    CCTGGGGGCC AGTGGATTTA CATGAGTGAT AACTACACAG ATGTGAATGG GGAGAAGGTA
    CTTCCGAAGG ATGATATTGA ATGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA
    GACCGAAAGC CAAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA
    CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AAGCACTGAA GAGGCACAGG
    CAGGCAGAGG CCGAGGGCGA GGGCTGGGAG TATGCCTCTC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCG
    CTGGAGAAGA CGGGACCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCATGGTG
    GATGACAAGA GCGAAGACTC CATGTCTGTT TCTACCCTGA GCTTTGGCGT TAACAGACCC
    ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGCTGCTA CCTGTACCAG
    GCCCGAGACC TGCCAGCCAT GGACAAGGAC TCCTTCTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCATC AAAGCCAGAA GACAGTGGTG GAGAAGAACA CCCTGAATCC CACCTGGGAT
    CAAACCCTCA TCTTCTATGA GATTGAGATC TTCGGCGAGC CAGCCAGTAT CGCCGAGCAT
    CCGCCCTGCA TCGTGGTGGA GCTCTATGAC CACGACACCT ATGGTGCTGA CGAGTTCATG
    GGTCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG
    ACCCGGGGCA GCCAGCCTGC CGGCGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG
    AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCATG AAACTTCAAG GATTTTGGAT
    GAGCTATGCA CGCCTGCCTT CTTCCTGCAG GGCTTCCTTC GGACGAGCAC GACGGAGGAC
    ACAGATTTGC CTTACCCACC ACCTCAAAGG GAGGCCAACA TCTACATGGT TCCCCAGAAC
    ATTAAGCCAG CTCTCCAACG TACTGCCATT GAGATCCTGG CCTGGGGCCT GCGGAACATG
    AAGAGCTATC AGATGGCCAG CATCTCTTCC CCCAGCCTCG TGGTGGAGTG TGGTGGGCAG
    ACAGTGCAGT CCTGTGTCAT CAGAAACCTT CGGAAGAACC CCAACTTTGA TGTCTGTACT
    CTCTTCATGG AAGTGATGTT ACCCAGAGAG GACCTCTACT GTCCACCTAT TGTGGTCAAG
    GTCATTGATA ATCGCCAGTT TGGCCGCCGG CCAGTTGTGG GTCAGTGTAC CATTCGCTCC
    CTGGAGAACT TCCTGTGCGA CCCATACTCA GCAGAGAGTC CATCCCCACA GGGTGGCCCA
    GATGATGTGA GCTTACTCAG CCCCGGGGAA GATGTGCTGA TTGACATCGA TGACAAGGAG
    CCTCTCATTC CTGTCCAGCT TACAGATGGG CTGTCAAGCT TGGGCCCCAC TAACCTGACG
    CCTTCTCCAT CCAGTCCTCA TAAGATCCTC CTGGAGGAGG AGTTCATTGA CTGGTGGAGC
    AAATTCTTTG CTTCTGTCGG GGAGAGGGAA AAGTGTGGTT CCTACCTGGA GAAGGATTTT
    GACACCTTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCTTTTGG AGGCCTGTCC
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAGGACTC AGGAGGAGAC AGATGATCCA
    TCTGTCATCG GAGAATTTAA GGGTCTCTTC AAAATTTATC CCCTCCCAGA AGATCCAGCC
    ATCCCCATGC CCCCGAGACA ATTCCATCAG CTGGCTGCTC AGGGTCCCCA GGAATGCTTG
    GTCCGTATTT ATATCGTCCG AGCATTTGGC TTACAGCCCA AGGACCCCAA TGGCAAGTGT
    GACCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC
    CCTTGCACCC TGGAGCCTGT ATTCGGAAAG ATGTTTGAAC TCACCTGCAC TCTACCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTACGAT TATGACCTGC TCTCCAAGGA TGAGAAGATT
    GGGGAGACGG TCATAGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGG
    CTCCCACAGA CCTACTGTGT CTCTGGACCA AATCAGTGGA GAGATCAGCT CCGCCCCTCC
    CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC AGGATCAAGG CCCCCGTGTA CCGGACAGAC
    CGAGTGACGT TTCAGGATAA GGACTACACC ATTGAGGAGA TAGAGGCTGG CAGACTCCCA
    AACCCACACC TGGGCCCAGT GGAGGAACGC TTAGCCCTGC ATGTCCTTCA GCAACAAGGC
    TTGGTTCCTG AGCATGTGGA GTCACGGCCT CTTTATAGTC CTCTGCAGCC AGATATCGAG
    CAGGGGAAGC TACAGATGTG GATTGACATA TTTCCAAAGG TGCTGGGCCG GCCTGGACCT
    CCCTTCAACA TCACCCCACG GAAAGCTAGA AGGTTTTTTC TGCGTTGTAT TATTTGGAAC
    ACAAAAGATG TGATCTTGGA TGACCTCAGT CTCACGGGGG AGAAGATGAG TGACATCTAT
    GTGAAAGGCT GGATGGTGGG ATTCGAAGAA CACAAGCAGA AGACAGATGT GCACTACCGC
    TCCCTGGGTG GGGAGGGCAA TTTCAACTGG AGGTTCGTGT TTCCCTTCGA CTATCTGCCT
    GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG GATGCCTTCT GGAGACTCGA CAAGACAGAG
    AGCAAGATCC CAGCACGTGT GGTCTTCCAG ATCTGGGACA ATGACAAATT CTCTTTTGAT
    GACTTTCTGG GCTCTCTGCA GCTGGATCTC AACCGAATGC CCAAGCCAGC CAAGACGGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACCTTCCACC CAGAATGGTT TGTGTCCCTT
    TTTGAGCAGA AGACAGTGAA AGGATGGTGG CCTTGTGTGA CAGAGGAGGG CGAGAAGAAG
    ATGTTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTTG CAGAAAGTGA ACATGAAGAG
    CGGCCTGCTG GCCAAGGTCG GGATGAACCC AACATGAATC CGAAGCTAGA GGATCCAAGG
    CGCCCCGATA CTTCTTTCCT GTGGTTCACC TCCCCGTACA AGACCATGAA GTTCATCTTG
    TGGCGACGCT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT CCTGCTCTTC
    CTGGGTGTCT TTGTCTACGC CTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTT
    CGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506229.1
    CDS256..6681
    Translation

    Target ORF information:

    RefSeq Version XM_006506166.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506166.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGTGGACGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC
    TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCA
    GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCTTATCGAG GCAGGGTTCT GTTGTCCCTG
    GAGACCAAAC TGGTGGAACA CAGTGAGCAG AAGGTGGAAG ACCTTCCTGC TGATGATATC
    CTGCGTGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA
    GCCACAATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC
    GGGAACAAGT TCGACACCAC CTGCCTGCCC TTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGATG GATGCCACTA CTATTACTTG CCTTGGGGCA ACGTGAAGCC CGTGGTGGTG
    CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAATTGAGA TCCAAAACCA GCTCCTCAGG
    GTCGCTGACC GCCTGGAAGC TAACCTGGAG CAGGTCCACT TGGCTCTGAA GGCACAGTGT
    TCCTCCGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC
    AGCCAGCCCC TGTGTGACAT CCACGAGATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG
    CTCCGGCTAC GCACCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCTCTGGC CTTGAAGCTT
    GGCCATAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT
    GCCCTGGCCG AGGAGCCCCA GAACAGCCTG CCGGACATCA TCATCTGGAT GCTGCAGGGT
    GACAAGCGTG TGGCCTACCA GCGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC
    CCCAGCTACT GCGGCAGGAA CTGTGGGAAG CTGCAGACCA TCTTTCTGAA ATATCCTATG
    GAAGGTATGC CTGGGGCCCG AATGCCCGTG CAGATTCGGA TCAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAATT CAATCAGTTT GCTGAGGGAA AGCTCTCTGT CTTTGCCGAA
    ACGTATGAGA ACCAGACAAA GCTGGCACTG GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGATGT CACAGGCAAG ATCAAACTAC CAAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGGCCTGGGC AGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GATGCTGACG CTGGTCATCT GAGCTTCGTG GAGGAGGTGT TCGAGAACCA GACCCGGCTC
    CCTGGGGGCC AGTGGATTTA CATGAGTGAT AACTACACAG ATGTGAATGG GGAGAAGGTA
    CTTCCGAAGG ATGATATTGA ATGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA
    GACCGAAAGC CAAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA
    CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AAGCACTGAA GAGGCACAGG
    CAGGCAGAGG CCGAGGGCGA GGGCTGGGAG TATGCCTCTC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCG
    CTGGAGAAGA CGGGACCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCATGGTG
    GATGACAAGA GCGAAGACTC CATGTCTGTT TCTACCCTGA GCTTTGGCGT TAACAGACCC
    ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGCTGCTA CCTGTACCAG
    GCCCGAGACC TGCCAGCCAT GGACAAGGAC TCCTTCTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCATC AAAGCCAGAA GACAGTGGTG GAGAAGAACA CCCTGAATCC CACCTGGGAT
    CAAACCCTCA TCTTCTATGA GATTGAGATC TTCGGCGAGC CAGCCAGTAT CGCCGAGCAT
    CCGCCCTGCA TCGTGGTGGA GCTCTATGAC CACGACACCT ATGGTGCTGA CGAGTTCATG
    GGTCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG
    ACCCGGGGCA GCCAGCCTGC CGGCGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG
    AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCATG AAACTTCAAG GATTTTGGAT
    GAGCTATGCA CGCCTGCCTT CTTCCTGCAG GGCTTCCTTC GGACGAGCAC GACGGAGGAC
    ACAGATTTGC CTTACCCACC ACCTCAAAGG GAGGCCAACA TCTACATGGT TCCCCAGAAC
    ATTAAGCCAG CTCTCCAACG TACTGCCATT GAGATCCTGG CCTGGGGCCT GCGGAACATG
    AAGAGCTATC AGATGGCCAG CATCTCTTCC CCCAGCCTCG TGGTGGAGTG TGGTGGGCAG
    ACAGTGCAGT CCTGTGTCAT CAGAAACCTT CGGAAGAACC CCAACTTTGA TGTCTGTACT
    CTCTTCATGG AAGTGATGTT ACCCAGAGAG GACCTCTACT GTCCACCTAT TGTGGTCAAG
    GTCATTGATA ATCGCCAGTT TGGCCGCCGG CCAGTTGTGG GTCAGTGTAC CATTCGCTCC
    CTGGAGAACT TCCTGTGCGA CCCATACTCA GCAGAGAGTC CATCCCCACA GGGTGGCCCA
    GATGATGTGA GCTTACTCAG CCCCGGGGAA GATGTGCTGA TTGACATCGA TGACAAGGAG
    CCTCTCATTC CTGTCCAGCT TACAGATGGG CTGTCAAGCT TGGGCCCCAC TAACCTGACG
    CCTTCTCCAT CCAGTCCTCA TAAGATCCTC CTGGAGGAGG AGTTCATTGA CTGGTGGAGC
    AAATTCTTTG CTTCTGTCGG GGAGAGGGAA AAGTGTGGTT CCTACCTGGA GAAGGATTTT
    GACACCTTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCTTTTGG AGGCCTGTCC
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAGGACTC AGGAGGAGAC AGATGATCCA
    TCTGTCATCG GAGAATTTAA GGGTCTCTTC AAAATTTATC CCCTCCCAGA AGATCCAGCC
    ATCCCCATGC CCCCGAGACA ATTCCATCAG CTGGCTGCTC AGGGTCCCCA GGAATGCTTG
    GTCCGTATTT ATATCGTCCG AGCATTTGGC TTACAGCCCA AGGACCCCAA TGGCAAGTGT
    GACCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC
    CCTTGCACCC TGGAGCCTGT ATTCGGAAAG ATGTTTGAAC TCACCTGCAC TCTACCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTACGAT TATGACCTGC TCTCCAAGGA TGAGAAGATT
    GGGGAGACGG TCATAGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGG
    CTCCCACAGA CCTACTGTGT CTCTGGACCA AATCAGTGGA GAGATCAGCT CCGCCCCTCC
    CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC AGGATCAAGG CCCCCGTGTA CCGGACAGAC
    CGAGTGACGT TTCAGGATAA GGACTACACC ATTGAGGAGA TAGAGGCTGG CAGACTCCCA
    AACCCACACC TGGGCCCAGT GGAGGAACGC TTAGCCCTGC ATGTCCTTCA GCAACAAGGC
    TTGGTTCCTG AGCATGTGGA GTCACGGCCT CTTTATAGTC CTCTGCAGCC AGATATCGAG
    CAGGGGAAGC TACAGATGTG GATTGACATA TTTCCAAAGG TGCTGGGCCG GCCTGGACCT
    CCCTTCAACA TCACCCCACG GAAAGCTAGA AGGTTTTTTC TGCGTTGTAT TATTTGGAAC
    ACAAAAGATG TGATCTTGGA TGACCTCAGT CTCACGGGGG AGAAGATGAG TGACATCTAT
    GTGAAAGGCT GGATGGTGGG ATTCGAAGAA CACAAGCAGA AGACAGATGT GCACTACCGC
    TCCCTGGGTG GGGAGGGCAA TTTCAACTGG AGGTTCGTGT TTCCCTTCGA CTATCTGCCT
    GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG GATGCCTTCT GGAGACTCGA CAAGACAGAG
    AGCAAGATCC CAGCACGTGT GGTCTTCCAG ATCTGGGACA ATGACAAATT CTCTTTTGAT
    GACTTTCTGG GCTCTCTGCA GCTGGATCTC AACCGAATGC CCAAGCCAGC CAAGACGGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACCTTCCACC CAGAATGGTT TGTGTCCCTT
    TTTGAGCAGA AGACAGTGAA AGGATGGTGG CCTTGTGTGA CAGAGGAGGG CGAGAAGAAG
    ATGTTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTTG CAGAAAGTGA ACATGAAGAG
    CGGCCTGCTG GCCAAGGTCG GGATGAACCC AACATGAATC CGAAGCTAGA GGATCCAAGG
    CGCCCCGATA CTTCTTTCCT GTGGTTCACC TCCCCGTACA AGACCATGAA GTTCATCTTG
    TGGCGACGCT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT CCTGCTCTTC
    CTGGGTGTCT TTGTCTACGC CTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTT
    CGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37295
    Clone ID Related Accession (Same CDS sequence) XM_006506167.2
    Accession Version XM_006506167.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGACGGA GGACACAGAT
    TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATTAAG
    CCAGCTCTCC AACGTACTGC CATTGAGATC CTGGCCTGGG GCCTGCGGAA CATGAAGAGC
    TATCAGATGG CCAGCATCTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG
    CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGATGTCTG TACTCTCTTC
    ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCAC CTATTGTGGT CAAGGTCATT
    GATAATCGCC AGTTTGGCCG CCGGCCAGTT GTGGGTCAGT GTACCATTCG CTCCCTGGAG
    AACTTCCTGT GCGACCCATA CTCAGCAGAG AGTCCATCCC CACAGGGTGG CCCAGATGAT
    GTGAGCTTAC TCAGCCCCGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCTCTC
    ATTCCTGTCC AGCTTACAGA TGGGCTGTCA AGCTTGGGCC CCACTAACCT GACGCCTTCT
    CCATCCAGTC CTCATAAGAT CCTCCTGGAG GAGGAGTTCA TTGACTGGTG GAGCAAATTC
    TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT GGTTCCTACC TGGAGAAGGA TTTTGACACC
    TTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCTT TTGGAGGCCT GTCCGACTTT
    TGTAACACCT TCAAGCTCTA CCGGGGCAGG ACTCAGGAGG AGACAGATGA TCCATCTGTC
    ATCGGAGAAT TTAAGGGTCT CTTCAAAATT TATCCCCTCC CAGAAGATCC AGCCATCCCC
    ATGCCCCCGA GACAATTCCA TCAGCTGGCT GCTCAGGGTC CCCAGGAATG CTTGGTCCGT
    ATTTATATCG TCCGAGCATT TGGCTTACAG CCCAAGGACC CCAATGGCAA GTGTGACCCT
    TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCTTGC
    ACCCTGGAGC CTGTATTCGG AAAGATGTTT GAACTCACCT GCACTCTACC TCTGGAGAAG
    GACCTGAAGA TCACACTCTA CGATTATGAC CTGCTCTCCA AGGATGAGAA GATTGGGGAG
    ACGGTCATAG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA
    CAGACCTACT GTGTCTCTGG ACCAAATCAG TGGAGAGATC AGCTCCGCCC CTCCCAGCTC
    CTCCACCTCT TCTGCCAGCA GCACAGGATC AAGGCCCCCG TGTACCGGAC AGACCGAGTG
    ACGTTTCAGG ATAAGGACTA CACCATTGAG GAGATAGAGG CTGGCAGACT CCCAAACCCA
    CACCTGGGCC CAGTGGAGGA ACGCTTAGCC CTGCATGTCC TTCAGCAACA AGGCTTGGTT
    CCTGAGCATG TGGAGTCACG GCCTCTTTAT AGTCCTCTGC AGCCAGATAT CGAGCAGGGG
    AAGCTACAGA TGTGGATTGA CATATTTCCA AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC
    AACATCACCC CACGGAAAGC TAGAAGGTTT TTTCTGCGTT GTATTATTTG GAACACAAAA
    GATGTGATCT TGGATGACCT CAGTCTCACG GGGGAGAAGA TGAGTGACAT CTATGTGAAA
    GGCTGGATGG TGGGATTCGA AGAACACAAG CAGAAGACAG ATGTGCACTA CCGCTCCCTG
    GGTGGGGAGG GCAATTTCAA CTGGAGGTTC GTGTTTCCCT TCGACTATCT GCCTGCTGAG
    CAAGTCTGTG CTGTTGCCAA GAAGGATGCC TTCTGGAGAC TCGACAAGAC AGAGAGCAAG
    ATCCCAGCAC GTGTGGTCTT CCAGATCTGG GACAATGACA AATTCTCTTT TGATGACTTT
    CTGGGCTCTC TGCAGCTGGA TCTCAACCGA ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG
    TGCTCCTTGG ACCAGCTGGA TGACACCTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG
    CAGAAGACAG TGAAAGGATG GTGGCCTTGT GTGACAGAGG AGGGCGAGAA GAAGATGTTG
    GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTTGCAGAAA GTGAACATGA AGAGCGGCCT
    GCTGGCCAAG GTCGGGATGA ACCCAACATG AATCCGAAGC TAGAGGATCC AAGGCGCCCC
    GATACTTCTT TCCTGTGGTT CACCTCCCCG TACAAGACCA TGAAGTTCAT CTTGTGGCGA
    CGCTTCCGGT GTGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT
    GTCTTTGTCT ACGCCTTCCC GAACTACGCT GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506230.1
    CDS255..6674
    Translation

    Target ORF information:

    RefSeq Version XM_006506167.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506167.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGACGGA GGACACAGAT
    TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATTAAG
    CCAGCTCTCC AACGTACTGC CATTGAGATC CTGGCCTGGG GCCTGCGGAA CATGAAGAGC
    TATCAGATGG CCAGCATCTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG
    CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGATGTCTG TACTCTCTTC
    ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCAC CTATTGTGGT CAAGGTCATT
    GATAATCGCC AGTTTGGCCG CCGGCCAGTT GTGGGTCAGT GTACCATTCG CTCCCTGGAG
    AACTTCCTGT GCGACCCATA CTCAGCAGAG AGTCCATCCC CACAGGGTGG CCCAGATGAT
    GTGAGCTTAC TCAGCCCCGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCTCTC
    ATTCCTGTCC AGCTTACAGA TGGGCTGTCA AGCTTGGGCC CCACTAACCT GACGCCTTCT
    CCATCCAGTC CTCATAAGAT CCTCCTGGAG GAGGAGTTCA TTGACTGGTG GAGCAAATTC
    TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT GGTTCCTACC TGGAGAAGGA TTTTGACACC
    TTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCTT TTGGAGGCCT GTCCGACTTT
    TGTAACACCT TCAAGCTCTA CCGGGGCAGG ACTCAGGAGG AGACAGATGA TCCATCTGTC
    ATCGGAGAAT TTAAGGGTCT CTTCAAAATT TATCCCCTCC CAGAAGATCC AGCCATCCCC
    ATGCCCCCGA GACAATTCCA TCAGCTGGCT GCTCAGGGTC CCCAGGAATG CTTGGTCCGT
    ATTTATATCG TCCGAGCATT TGGCTTACAG CCCAAGGACC CCAATGGCAA GTGTGACCCT
    TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCTTGC
    ACCCTGGAGC CTGTATTCGG AAAGATGTTT GAACTCACCT GCACTCTACC TCTGGAGAAG
    GACCTGAAGA TCACACTCTA CGATTATGAC CTGCTCTCCA AGGATGAGAA GATTGGGGAG
    ACGGTCATAG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA
    CAGACCTACT GTGTCTCTGG ACCAAATCAG TGGAGAGATC AGCTCCGCCC CTCCCAGCTC
    CTCCACCTCT TCTGCCAGCA GCACAGGATC AAGGCCCCCG TGTACCGGAC AGACCGAGTG
    ACGTTTCAGG ATAAGGACTA CACCATTGAG GAGATAGAGG CTGGCAGACT CCCAAACCCA
    CACCTGGGCC CAGTGGAGGA ACGCTTAGCC CTGCATGTCC TTCAGCAACA AGGCTTGGTT
    CCTGAGCATG TGGAGTCACG GCCTCTTTAT AGTCCTCTGC AGCCAGATAT CGAGCAGGGG
    AAGCTACAGA TGTGGATTGA CATATTTCCA AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC
    AACATCACCC CACGGAAAGC TAGAAGGTTT TTTCTGCGTT GTATTATTTG GAACACAAAA
    GATGTGATCT TGGATGACCT CAGTCTCACG GGGGAGAAGA TGAGTGACAT CTATGTGAAA
    GGCTGGATGG TGGGATTCGA AGAACACAAG CAGAAGACAG ATGTGCACTA CCGCTCCCTG
    GGTGGGGAGG GCAATTTCAA CTGGAGGTTC GTGTTTCCCT TCGACTATCT GCCTGCTGAG
    CAAGTCTGTG CTGTTGCCAA GAAGGATGCC TTCTGGAGAC TCGACAAGAC AGAGAGCAAG
    ATCCCAGCAC GTGTGGTCTT CCAGATCTGG GACAATGACA AATTCTCTTT TGATGACTTT
    CTGGGCTCTC TGCAGCTGGA TCTCAACCGA ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG
    TGCTCCTTGG ACCAGCTGGA TGACACCTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG
    CAGAAGACAG TGAAAGGATG GTGGCCTTGT GTGACAGAGG AGGGCGAGAA GAAGATGTTG
    GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTTGCAGAAA GTGAACATGA AGAGCGGCCT
    GCTGGCCAAG GTCGGGATGA ACCCAACATG AATCCGAAGC TAGAGGATCC AAGGCGCCCC
    GATACTTCTT TCCTGTGGTT CACCTCCCCG TACAAGACCA TGAAGTTCAT CTTGTGGCGA
    CGCTTCCGGT GTGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT
    GTCTTTGTCT ACGCCTTCCC GAACTACGCT GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37296
    Clone ID Related Accession (Same CDS sequence) XM_006506168.2
    Accession Version XM_006506168.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    GAGGAGGAGT TCATTGACTG GTGGAGCAAA TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG
    TGTGGTTCCT ACCTGGAGAA GGATTTTGAC ACCTTGAAGG TCTATGACAC ACAGCTGGAG
    AATGTGGAGG CTTTTGGAGG CCTGTCCGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AGGACTCAGG AGGAGACAGA TGATCCATCT GTCATCGGAG AATTTAAGGG TCTCTTCAAA
    ATTTATCCCC TCCCAGAAGA TCCAGCCATC CCCATGCCCC CGAGACAATT CCATCAGCTG
    GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC CGTATTTATA TCGTCCGAGC ATTTGGCTTA
    CAGCCCAAGG ACCCCAATGG CAAGTGTGAC CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATCCCT TGCACCCTGG AGCCTGTATT CGGAAAGATG
    TTTGAACTCA CCTGCACTCT ACCTCTGGAG AAGGACCTGA AGATCACACT CTACGATTAT
    GACCTGCTCT CCAAGGATGA GAAGATTGGG GAGACGGTCA TAGACCTGGA GAACAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC CCACAGACCT ACTGTGTCTC TGGACCAAAT
    CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCACAGG
    ATCAAGGCCC CCGTGTACCG GACAGACCGA GTGACGTTTC AGGATAAGGA CTACACCATT
    GAGGAGATAG AGGCTGGCAG ACTCCCAAAC CCACACCTGG GCCCAGTGGA GGAACGCTTA
    GCCCTGCATG TCCTTCAGCA ACAAGGCTTG GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT
    TATAGTCCTC TGCAGCCAGA TATCGAGCAG GGGAAGCTAC AGATGTGGAT TGACATATTT
    CCAAAGGTGC TGGGCCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAA AGCTAGAAGG
    TTTTTTCTGC GTTGTATTAT TTGGAACACA AAAGATGTGA TCTTGGATGA CCTCAGTCTC
    ACGGGGGAGA AGATGAGTGA CATCTATGTG AAAGGCTGGA TGGTGGGATT CGAAGAACAC
    AAGCAGAAGA CAGATGTGCA CTACCGCTCC CTGGGTGGGG AGGGCAATTT CAACTGGAGG
    TTCGTGTTTC CCTTCGACTA TCTGCCTGCT GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT
    GCCTTCTGGA GACTCGACAA GACAGAGAGC AAGATCCCAG CACGTGTGGT CTTCCAGATC
    TGGGACAATG ACAAATTCTC TTTTGATGAC TTTCTGGGCT CTCTGCAGCT GGATCTCAAC
    CGAATGCCCA AGCCAGCCAA GACGGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACC
    TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT
    TGTGTGACAG AGGAGGGCGA GAAGAAGATG TTGGCGGGCA AGCTGGAAAT GACCTTGGAG
    ATTGTTGCAG AAAGTGAACA TGAAGAGCGG CCTGCTGGCC AAGGTCGGGA TGAACCCAAC
    ATGAATCCGA AGCTAGAGGA TCCAAGGCGC CCCGATACTT CTTTCCTGTG GTTCACCTCC
    CCGTACAAGA CCATGAAGTT CATCTTGTGG CGACGCTTCC GGTGTGCCAT CATCCTCTTC
    ATCATCCTCT TCATCCTCCT GCTCTTCCTG GGTGTCTTTG TCTACGCCTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTTCGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506231.1
    CDS256..6648
    Translation

    Target ORF information:

    RefSeq Version XM_006506168.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506168.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGCTATG CACGCCTGCC
    TTCTTCCTGC AGGGCTTCCT TCGGACGAGC ACGACGGAGG ACACAGATTT GCCTTACCCA
    CCACCTCAAA GGGAGGCCAA CATCTACATG GTTCCCCAGA ACATTAAGCC AGCTCTCCAA
    CGTACTGCCA TTGAGATCCT GGCCTGGGGC CTGCGGAACA TGAAGAGCTA TCAGATGGCC
    AGCATCTCTT CCCCCAGCCT CGTGGTGGAG TGTGGTGGGC AGACAGTGCA GTCCTGTGTC
    ATCAGAAACC TTCGGAAGAA CCCCAACTTT GATGTCTGTA CTCTCTTCAT GGAAGTGATG
    TTACCCAGAG AGGACCTCTA CTGTCCACCT ATTGTGGTCA AGGTCATTGA TAATCGCCAG
    TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT ACCATTCGCT CCCTGGAGAA CTTCCTGTGC
    GACCCATACT CAGCAGAGAG TCCATCCCCA CAGGGTGGCC CAGATGATGT GAGCTTACTC
    AGCCCCGGGG AAGATGTGCT GATTGACATC GATGACAAGG AGCCTCTCAT TCCTGTCCAG
    GAGGAGGAGT TCATTGACTG GTGGAGCAAA TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG
    TGTGGTTCCT ACCTGGAGAA GGATTTTGAC ACCTTGAAGG TCTATGACAC ACAGCTGGAG
    AATGTGGAGG CTTTTGGAGG CCTGTCCGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AGGACTCAGG AGGAGACAGA TGATCCATCT GTCATCGGAG AATTTAAGGG TCTCTTCAAA
    ATTTATCCCC TCCCAGAAGA TCCAGCCATC CCCATGCCCC CGAGACAATT CCATCAGCTG
    GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC CGTATTTATA TCGTCCGAGC ATTTGGCTTA
    CAGCCCAAGG ACCCCAATGG CAAGTGTGAC CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATCCCT TGCACCCTGG AGCCTGTATT CGGAAAGATG
    TTTGAACTCA CCTGCACTCT ACCTCTGGAG AAGGACCTGA AGATCACACT CTACGATTAT
    GACCTGCTCT CCAAGGATGA GAAGATTGGG GAGACGGTCA TAGACCTGGA GAACAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC CCACAGACCT ACTGTGTCTC TGGACCAAAT
    CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCACAGG
    ATCAAGGCCC CCGTGTACCG GACAGACCGA GTGACGTTTC AGGATAAGGA CTACACCATT
    GAGGAGATAG AGGCTGGCAG ACTCCCAAAC CCACACCTGG GCCCAGTGGA GGAACGCTTA
    GCCCTGCATG TCCTTCAGCA ACAAGGCTTG GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT
    TATAGTCCTC TGCAGCCAGA TATCGAGCAG GGGAAGCTAC AGATGTGGAT TGACATATTT
    CCAAAGGTGC TGGGCCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAA AGCTAGAAGG
    TTTTTTCTGC GTTGTATTAT TTGGAACACA AAAGATGTGA TCTTGGATGA CCTCAGTCTC
    ACGGGGGAGA AGATGAGTGA CATCTATGTG AAAGGCTGGA TGGTGGGATT CGAAGAACAC
    AAGCAGAAGA CAGATGTGCA CTACCGCTCC CTGGGTGGGG AGGGCAATTT CAACTGGAGG
    TTCGTGTTTC CCTTCGACTA TCTGCCTGCT GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT
    GCCTTCTGGA GACTCGACAA GACAGAGAGC AAGATCCCAG CACGTGTGGT CTTCCAGATC
    TGGGACAATG ACAAATTCTC TTTTGATGAC TTTCTGGGCT CTCTGCAGCT GGATCTCAAC
    CGAATGCCCA AGCCAGCCAA GACGGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACC
    TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT
    TGTGTGACAG AGGAGGGCGA GAAGAAGATG TTGGCGGGCA AGCTGGAAAT GACCTTGGAG
    ATTGTTGCAG AAAGTGAACA TGAAGAGCGG CCTGCTGGCC AAGGTCGGGA TGAACCCAAC
    ATGAATCCGA AGCTAGAGGA TCCAAGGCGC CCCGATACTT CTTTCCTGTG GTTCACCTCC
    CCGTACAAGA CCATGAAGTT CATCTTGTGG CGACGCTTCC GGTGTGCCAT CATCCTCTTC
    ATCATCCTCT TCATCCTCCT GCTCTTCCTG GGTGTCTTTG TCTACGCCTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTTCGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37297
    Clone ID Related Accession (Same CDS sequence) XM_006506169.2
    Accession Version XM_006506169.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTACTCA AGAGTCCACA AGCCACTGAC CTGGACGACA ACCTGGGCTT CCTCCCCACT
    TTTGGGCCCT GCTATGTGAA CCTCTATGGC AGTCCCCGAG AGTTCACGGG CTTCCCAGAT
    CCTTATGCAG AGCTCAACAC AGGAAAGGGA GAAGGTGTGG CTTATCGAGG CAGGGTTCTG
    TTGTCCCTGG AGACCAAACT GGTGGAACAC AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT
    GATGATATCC TGCGTGTGGA GAAGTACCTG CGGAGGCGCA AATATTCCCT CTTTGCTGCC
    TTCTACTCAG CCACAATGCT GCAGGATGTG GATGACGCCA TCCAGTTCGA GGTCAGCATT
    GGGAACTACG GGAACAAGTT CGACACCACC TGCCTGCCCT TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGATGG ATGCCACTAC TATTACTTGC CTTGGGGCAA CGTGAAGCCC
    GTGGTGGTGC TGTCCTCATA CTGGGAAGAC ATCAGCCATC GAATTGAGAT CCAAAACCAG
    CTCCTCAGGG TCGCTGACCG CCTGGAAGCT AACCTGGAGC AGGTCCACTT GGCTCTGAAG
    GCACAGTGTT CCTCCGAGGA TGTGGACGCC CTGGTGGCTC AGTTGACAGA TGAGCTCCTT
    GCAGACTGCA GCCAGCCCCT GTGTGACATC CACGAGATAC CTTCTGCCAC CCACCTTGAT
    CAGTACCTGC TCCGGCTACG CACCCGTCAC CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC
    TTGAAGCTTG GCCATAGTGA GCTCTCCACT GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG
    CATCTCCGTG CCCTGGCCGA GGAGCCCCAG AACAGCCTGC CGGACATCAT CATCTGGATG
    CTGCAGGGTG ACAAGCGTGT GGCCTACCAG CGGGTGCCTG CCCATGAAGT CCTCTTCTCC
    CGGCGGGGCC CCAGCTACTG CGGCAGGAAC TGTGGGAAGC TGCAGACCAT CTTTCTGAAA
    TATCCTATGG AAGGTATGCC TGGGGCCCGA ATGCCCGTGC AGATTCGGAT CAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAATTC AATCAGTTTG CTGAGGGAAA GCTCTCTGTC
    TTTGCCGAAA CGTATGAGAA CCAGACAAAG CTGGCACTGG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACAGGCAAGA TCAAACTACC AAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA GGAGATTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ATGCTGACGC TGGTCATCTG AGCTTCGTGG AGGAGGTGTT CGAGAACCAG
    ACCCGGCTCC CTGGGGGCCA GTGGATTTAC ATGAGTGATA ACTACACAGA TGTGAATGGG
    GAGAAGGTAC TTCCGAAGGA TGATATTGAA TGCCCGCTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCTGTT GATGAACAAG GCTGGGAGTA CAGCATCACC
    ATACCCCCAG ACCGAAAGCC AAAACACTGG GTCCCTGTTG AGAAGATGTA TTACACTCAC
    AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG AGGGACCTCA GCCAGATGGA AGCACTGAAG
    AGGCACAGGC AGGCAGAGGC CGAGGGCGAG GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACAGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCGC TGGAGAAGAC GGGACCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCATGGTGG ATGACAAGAG CGAAGACTCC ATGTCTGTTT CTACCCTGAG CTTTGGCGTT
    AACAGACCCA CAATCTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT GCGCTGCTAC
    CTGTACCAGG CCCGAGACCT GCCAGCCATG GACAAGGACT CCTTCTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCATCA AAGCCAGAAG ACAGTGGTGG AGAAGAACAC CCTGAATCCC
    ACCTGGGATC AAACCCTCAT CTTCTATGAG ATTGAGATCT TCGGCGAGCC AGCCAGTATC
    GCCGAGCATC CGCCCTGCAT CGTGGTGGAG CTCTATGACC ACGACACCTA TGGTGCTGAC
    GAGTTCATGG GTCGCTGTAT TTGTCAGCCA AGCCTGGAAC GGATGCCCCG CCTGGCCTGG
    TTCCCACTGA CCCGGGGCAG CCAGCCTGCC GGCGAGCTGC TGGCAGCGTT CGAGCTTATC
    CAGAGAGAGA AGCCAGCCAT CCATCACATC CCTGGCTTTG AGATGCATGA AACTTCAAGG
    ATTTTGGATG AGCTATGCAC GCCTGCCTTC TTCCTGCAGG GCTTCCTTCG GACGAGCACG
    ACGGAGGACA CAGATTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TTAAGCCAGC TCTCCAACGT ACTGCCATTG AGATCCTGGC CTGGGGCCTG
    CGGAACATGA AGAGCTATCA GATGGCCAGC ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT
    GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAT
    GTCTGTACTC TCTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCACCTATT
    GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCCGGC CAGTTGTGGG TCAGTGTACC
    ATTCGCTCCC TGGAGAACTT CCTGTGCGAC CCATACTCAG CAGAGAGTCC ATCCCCACAG
    GGTGGCCCAG ATGATGTGAG CTTACTCAGC CCCGGGGAAG ATGTGCTGAT TGACATCGAT
    GACAAGGAGC CTCTCATTCC TGTCCAGCTT ACAGATGGGC TGTCAAGCTT GGGCCCCACT
    AACCTGACGC CTTCTCCATC CAGTCCTCAT AAGATCCTCC TGGAGGAGGA GTTCATTGAC
    TGGTGGAGCA AATTCTTTGC TTCTGTCGGG GAGAGGGAAA AGTGTGGTTC CTACCTGGAG
    AAGGATTTTG ACACCTTGAA GGTCTATGAC ACACAGCTGG AGAATGTGGA GGCTTTTGGA
    GGCCTGTCCG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAGGACTCA GGAGGAGACA
    GATGATCCAT CTGTCATCGG AGAATTTAAG GGTCTCTTCA AAATTTATCC CCTCCCAGAA
    GATCCAGCCA TCCCCATGCC CCCGAGACAA TTCCATCAGC TGGCTGCTCA GGGTCCCCAG
    GAATGCTTGG TCCGTATTTA TATCGTCCGA GCATTTGGCT TACAGCCCAA GGACCCCAAT
    GGCAAGTGTG ACCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATCC CTTGCACCCT GGAGCCTGTA TTCGGAAAGA TGTTTGAACT CACCTGCACT
    CTACCTCTGG AGAAGGACCT GAAGATCACA CTCTACGATT ATGACCTGCT CTCCAAGGAT
    GAGAAGATTG GGGAGACGGT CATAGACCTG GAGAACAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGTGGGC TCCCACAGAC CTACTGTGTC TCTGGACCAA ATCAGTGGAG AGATCAGCTC
    CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC CAGCAGCACA GGATCAAGGC CCCCGTGTAC
    CGGACAGACC GAGTGACGTT TCAGGATAAG GACTACACCA TTGAGGAGAT AGAGGCTGGC
    AGACTCCCAA ACCCACACCT GGGCCCAGTG GAGGAACGCT TAGCCCTGCA TGTCCTTCAG
    CAACAAGGCT TGGTTCCTGA GCATGTGGAG TCACGGCCTC TTTATAGTCC TCTGCAGCCA
    GATATCGAGC AGGGGAAGCT ACAGATGTGG ATTGACATAT TTCCAAAGGT GCTGGGCCGG
    CCTGGACCTC CCTTCAACAT CACCCCACGG AAAGCTAGAA GGTTTTTTCT GCGTTGTATT
    ATTTGGAACA CAAAAGATGT GATCTTGGAT GACCTCAGTC TCACGGGGGA GAAGATGAGT
    GACATCTATG TGAAAGGCTG GATGGTGGGA TTCGAAGAAC ACAAGCAGAA GACAGATGTG
    CACTACCGCT CCCTGGGTGG GGAGGGCAAT TTCAACTGGA GGTTCGTGTT TCCCTTCGAC
    TATCTGCCTG CTGAGCAAGT CTGTGCTGTT GCCAAGAAGG ATGCCTTCTG GAGACTCGAC
    AAGACAGAGA GCAAGATCCC AGCACGTGTG GTCTTCCAGA TCTGGGACAA TGACAAATTC
    TCTTTTGATG ACTTTCTGGG CTCTCTGCAG CTGGATCTCA ACCGAATGCC CAAGCCAGCC
    AAGACGGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CCTTCCACCC AGAATGGTTT
    GTGTCCCTTT TTGAGCAGAA GACAGTGAAA GGATGGTGGC CTTGTGTGAC AGAGGAGGGC
    GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA ATGACCTTGG AGATTGTTGC AGAAAGTGAA
    CATGAAGAGC GGCCTGCTGG CCAAGGTCGG GATGAACCCA ACATGAATCC GAAGCTAGAG
    GATCCAAGGC GCCCCGATAC TTCTTTCCTG TGGTTCACCT CCCCGTACAA GACCATGAAG
    TTCATCTTGT GGCGACGCTT CCGGTGTGCC ATCATCCTCT TCATCATCCT CTTCATCCTC
    CTGCTCTTCC TGGGTGTCTT TGTCTACGCC TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506232.1
    CDS256..6630
    Translation

    Target ORF information:

    RefSeq Version XM_006506169.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506169.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTACTCA AGAGTCCACA AGCCACTGAC CTGGACGACA ACCTGGGCTT CCTCCCCACT
    TTTGGGCCCT GCTATGTGAA CCTCTATGGC AGTCCCCGAG AGTTCACGGG CTTCCCAGAT
    CCTTATGCAG AGCTCAACAC AGGAAAGGGA GAAGGTGTGG CTTATCGAGG CAGGGTTCTG
    TTGTCCCTGG AGACCAAACT GGTGGAACAC AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT
    GATGATATCC TGCGTGTGGA GAAGTACCTG CGGAGGCGCA AATATTCCCT CTTTGCTGCC
    TTCTACTCAG CCACAATGCT GCAGGATGTG GATGACGCCA TCCAGTTCGA GGTCAGCATT
    GGGAACTACG GGAACAAGTT CGACACCACC TGCCTGCCCT TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGATGG ATGCCACTAC TATTACTTGC CTTGGGGCAA CGTGAAGCCC
    GTGGTGGTGC TGTCCTCATA CTGGGAAGAC ATCAGCCATC GAATTGAGAT CCAAAACCAG
    CTCCTCAGGG TCGCTGACCG CCTGGAAGCT AACCTGGAGC AGGTCCACTT GGCTCTGAAG
    GCACAGTGTT CCTCCGAGGA TGTGGACGCC CTGGTGGCTC AGTTGACAGA TGAGCTCCTT
    GCAGACTGCA GCCAGCCCCT GTGTGACATC CACGAGATAC CTTCTGCCAC CCACCTTGAT
    CAGTACCTGC TCCGGCTACG CACCCGTCAC CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC
    TTGAAGCTTG GCCATAGTGA GCTCTCCACT GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG
    CATCTCCGTG CCCTGGCCGA GGAGCCCCAG AACAGCCTGC CGGACATCAT CATCTGGATG
    CTGCAGGGTG ACAAGCGTGT GGCCTACCAG CGGGTGCCTG CCCATGAAGT CCTCTTCTCC
    CGGCGGGGCC CCAGCTACTG CGGCAGGAAC TGTGGGAAGC TGCAGACCAT CTTTCTGAAA
    TATCCTATGG AAGGTATGCC TGGGGCCCGA ATGCCCGTGC AGATTCGGAT CAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAATTC AATCAGTTTG CTGAGGGAAA GCTCTCTGTC
    TTTGCCGAAA CGTATGAGAA CCAGACAAAG CTGGCACTGG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACAGGCAAGA TCAAACTACC AAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA GGAGATTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ATGCTGACGC TGGTCATCTG AGCTTCGTGG AGGAGGTGTT CGAGAACCAG
    ACCCGGCTCC CTGGGGGCCA GTGGATTTAC ATGAGTGATA ACTACACAGA TGTGAATGGG
    GAGAAGGTAC TTCCGAAGGA TGATATTGAA TGCCCGCTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCTGTT GATGAACAAG GCTGGGAGTA CAGCATCACC
    ATACCCCCAG ACCGAAAGCC AAAACACTGG GTCCCTGTTG AGAAGATGTA TTACACTCAC
    AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG AGGGACCTCA GCCAGATGGA AGCACTGAAG
    AGGCACAGGC AGGCAGAGGC CGAGGGCGAG GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACAGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCGC TGGAGAAGAC GGGACCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCATGGTGG ATGACAAGAG CGAAGACTCC ATGTCTGTTT CTACCCTGAG CTTTGGCGTT
    AACAGACCCA CAATCTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT GCGCTGCTAC
    CTGTACCAGG CCCGAGACCT GCCAGCCATG GACAAGGACT CCTTCTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCATCA AAGCCAGAAG ACAGTGGTGG AGAAGAACAC CCTGAATCCC
    ACCTGGGATC AAACCCTCAT CTTCTATGAG ATTGAGATCT TCGGCGAGCC AGCCAGTATC
    GCCGAGCATC CGCCCTGCAT CGTGGTGGAG CTCTATGACC ACGACACCTA TGGTGCTGAC
    GAGTTCATGG GTCGCTGTAT TTGTCAGCCA AGCCTGGAAC GGATGCCCCG CCTGGCCTGG
    TTCCCACTGA CCCGGGGCAG CCAGCCTGCC GGCGAGCTGC TGGCAGCGTT CGAGCTTATC
    CAGAGAGAGA AGCCAGCCAT CCATCACATC CCTGGCTTTG AGATGCATGA AACTTCAAGG
    ATTTTGGATG AGCTATGCAC GCCTGCCTTC TTCCTGCAGG GCTTCCTTCG GACGAGCACG
    ACGGAGGACA CAGATTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TTAAGCCAGC TCTCCAACGT ACTGCCATTG AGATCCTGGC CTGGGGCCTG
    CGGAACATGA AGAGCTATCA GATGGCCAGC ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT
    GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAT
    GTCTGTACTC TCTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCACCTATT
    GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCCGGC CAGTTGTGGG TCAGTGTACC
    ATTCGCTCCC TGGAGAACTT CCTGTGCGAC CCATACTCAG CAGAGAGTCC ATCCCCACAG
    GGTGGCCCAG ATGATGTGAG CTTACTCAGC CCCGGGGAAG ATGTGCTGAT TGACATCGAT
    GACAAGGAGC CTCTCATTCC TGTCCAGCTT ACAGATGGGC TGTCAAGCTT GGGCCCCACT
    AACCTGACGC CTTCTCCATC CAGTCCTCAT AAGATCCTCC TGGAGGAGGA GTTCATTGAC
    TGGTGGAGCA AATTCTTTGC TTCTGTCGGG GAGAGGGAAA AGTGTGGTTC CTACCTGGAG
    AAGGATTTTG ACACCTTGAA GGTCTATGAC ACACAGCTGG AGAATGTGGA GGCTTTTGGA
    GGCCTGTCCG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAGGACTCA GGAGGAGACA
    GATGATCCAT CTGTCATCGG AGAATTTAAG GGTCTCTTCA AAATTTATCC CCTCCCAGAA
    GATCCAGCCA TCCCCATGCC CCCGAGACAA TTCCATCAGC TGGCTGCTCA GGGTCCCCAG
    GAATGCTTGG TCCGTATTTA TATCGTCCGA GCATTTGGCT TACAGCCCAA GGACCCCAAT
    GGCAAGTGTG ACCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATCC CTTGCACCCT GGAGCCTGTA TTCGGAAAGA TGTTTGAACT CACCTGCACT
    CTACCTCTGG AGAAGGACCT GAAGATCACA CTCTACGATT ATGACCTGCT CTCCAAGGAT
    GAGAAGATTG GGGAGACGGT CATAGACCTG GAGAACAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGTGGGC TCCCACAGAC CTACTGTGTC TCTGGACCAA ATCAGTGGAG AGATCAGCTC
    CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC CAGCAGCACA GGATCAAGGC CCCCGTGTAC
    CGGACAGACC GAGTGACGTT TCAGGATAAG GACTACACCA TTGAGGAGAT AGAGGCTGGC
    AGACTCCCAA ACCCACACCT GGGCCCAGTG GAGGAACGCT TAGCCCTGCA TGTCCTTCAG
    CAACAAGGCT TGGTTCCTGA GCATGTGGAG TCACGGCCTC TTTATAGTCC TCTGCAGCCA
    GATATCGAGC AGGGGAAGCT ACAGATGTGG ATTGACATAT TTCCAAAGGT GCTGGGCCGG
    CCTGGACCTC CCTTCAACAT CACCCCACGG AAAGCTAGAA GGTTTTTTCT GCGTTGTATT
    ATTTGGAACA CAAAAGATGT GATCTTGGAT GACCTCAGTC TCACGGGGGA GAAGATGAGT
    GACATCTATG TGAAAGGCTG GATGGTGGGA TTCGAAGAAC ACAAGCAGAA GACAGATGTG
    CACTACCGCT CCCTGGGTGG GGAGGGCAAT TTCAACTGGA GGTTCGTGTT TCCCTTCGAC
    TATCTGCCTG CTGAGCAAGT CTGTGCTGTT GCCAAGAAGG ATGCCTTCTG GAGACTCGAC
    AAGACAGAGA GCAAGATCCC AGCACGTGTG GTCTTCCAGA TCTGGGACAA TGACAAATTC
    TCTTTTGATG ACTTTCTGGG CTCTCTGCAG CTGGATCTCA ACCGAATGCC CAAGCCAGCC
    AAGACGGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CCTTCCACCC AGAATGGTTT
    GTGTCCCTTT TTGAGCAGAA GACAGTGAAA GGATGGTGGC CTTGTGTGAC AGAGGAGGGC
    GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA ATGACCTTGG AGATTGTTGC AGAAAGTGAA
    CATGAAGAGC GGCCTGCTGG CCAAGGTCGG GATGAACCCA ACATGAATCC GAAGCTAGAG
    GATCCAAGGC GCCCCGATAC TTCTTTCCTG TGGTTCACCT CCCCGTACAA GACCATGAAG
    TTCATCTTGT GGCGACGCTT CCGGTGTGCC ATCATCCTCT TCATCATCCT CTTCATCCTC
    CTGCTCTTCC TGGGTGTCTT TGTCTACGCC TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37298
    Clone ID Related Accession (Same CDS sequence) XM_006506170.2
    Accession Version XM_006506170.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGTGGACGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC
    TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCA
    GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCTTATCGAG GCAGGGTTCT GTTGTCCCTG
    GAGACCAAAC TGGTGGAACA CAGTGAGCAG AAGGTGGAAG ACCTTCCTGC TGATGATATC
    CTGCGTGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA
    GCCACAATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC
    GGGAACAAGT TCGACACCAC CTGCCTGCCC TTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGATG GATGCCACTA CTATTACTTG CCTTGGGGCA ACGTGAAGCC CGTGGTGGTG
    CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAATTGAGA TCCAAAACCA GCTCCTCAGG
    GTCGCTGACC GCCTGGAAGC TAACCTGGAG CAGGTCCACT TGGCTCTGAA GGCACAGTGT
    TCCTCCGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC
    AGCCAGCCCC TGTGTGACAT CCACGAGATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG
    CTCCGGCTAC GCACCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCTCTGGC CTTGAAGCTT
    GGCCATAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT
    GCCCTGGCCG AGGAGCCCCA GAACAGCCTG CCGGACATCA TCATCTGGAT GCTGCAGGGT
    GACAAGCGTG TGGCCTACCA GCGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC
    CCCAGCTACT GCGGCAGGAA CTGTGGGAAG CTGCAGACCA TCTTTCTGAA ATATCCTATG
    GAAGGTATGC CTGGGGCCCG AATGCCCGTG CAGATTCGGA TCAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAATT CAATCAGTTT GCTGAGGGAA AGCTCTCTGT CTTTGCCGAA
    ACGTATGAGA ACCAGACAAA GCTGGCACTG GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGATGT CACAGGCAAG ATCAAACTAC CAAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGGCCTGGGC AGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GATGCTGACG CTGGTCATCT GAGCTTCGTG GAGGAGGTGT TCGAGAACCA GACCCGGCTC
    CCTGGGGGCC AGTGGATTTA CATGAGTGAT AACTACACAG ATGTGAATGG GGAGAAGGTA
    CTTCCGAAGG ATGATATTGA ATGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA
    GACCGAAAGC CAAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA
    CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AAGCACTGAA GAGGCACAGG
    CAGGCAGAGG CCGAGGGCGA GGGCTGGGAG TATGCCTCTC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCG
    CTGGAGAAGA CGGGACCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCATGGTG
    GATGACAAGA GCGAAGACTC CATGTCTGTT TCTACCCTGA GCTTTGGCGT TAACAGACCC
    ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGCTGCTA CCTGTACCAG
    GCCCGAGACC TGCCAGCCAT GGACAAGGAC TCCTTCTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCATC AAAGCCAGAA GACAGTGGTG GAGAAGAACA CCCTGAATCC CACCTGGGAT
    CAAACCCTCA TCTTCTATGA GATTGAGATC TTCGGCGAGC CAGCCAGTAT CGCCGAGCAT
    CCGCCCTGCA TCGTGGTGGA GCTCTATGAC CACGACACCT ATGGTGCTGA CGAGTTCATG
    GGTCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG
    ACCCGGGGCA GCCAGCCTGC CGGCGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG
    AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCATG AAACTTCAAG GATTTTGGAT
    GAGACGGAGG ACACAGATTT GCCTTACCCA CCACCTCAAA GGGAGGCCAA CATCTACATG
    GTTCCCCAGA ACATTAAGCC AGCTCTCCAA CGTACTGCCA TTGAGATCCT GGCCTGGGGC
    CTGCGGAACA TGAAGAGCTA TCAGATGGCC AGCATCTCTT CCCCCAGCCT CGTGGTGGAG
    TGTGGTGGGC AGACAGTGCA GTCCTGTGTC ATCAGAAACC TTCGGAAGAA CCCCAACTTT
    GATGTCTGTA CTCTCTTCAT GGAAGTGATG TTACCCAGAG AGGACCTCTA CTGTCCACCT
    ATTGTGGTCA AGGTCATTGA TAATCGCCAG TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT
    ACCATTCGCT CCCTGGAGAA CTTCCTGTGC GACCCATACT CAGCAGAGAG TCCATCCCCA
    CAGGGTGGCC CAGATGATGT GAGCTTACTC AGCCCCGGGG AAGATGTGCT GATTGACATC
    GATGACAAGG AGCCTCTCAT TCCTGTCCAG CTTACAGATG GGCTGTCAAG CTTGGGCCCC
    ACTAACCTGA CGCCTTCTCC ATCCAGTCCT CATGAGGAGG AGTTCATTGA CTGGTGGAGC
    AAATTCTTTG CTTCTGTCGG GGAGAGGGAA AAGTGTGGTT CCTACCTGGA GAAGGATTTT
    GACACCTTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCTTTTGG AGGCCTGTCC
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAGGACTC AGGAGGAGAC AGATGATCCA
    TCTGTCATCG GAGAATTTAA GGGTCTCTTC AAAATTTATC CCCTCCCAGA AGATCCAGCC
    ATCCCCATGC CCCCGAGACA ATTCCATCAG CTGGCTGCTC AGGGTCCCCA GGAATGCTTG
    GTCCGTATTT ATATCGTCCG AGCATTTGGC TTACAGCCCA AGGACCCCAA TGGCAAGTGT
    GACCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC
    CCTTGCACCC TGGAGCCTGT ATTCGGAAAG ATGTTTGAAC TCACCTGCAC TCTACCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTACGAT TATGACCTGC TCTCCAAGGA TGAGAAGATT
    GGGGAGACGG TCATAGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGG
    CTCCCACAGA CCTACTGTGT CTCTGGACCA AATCAGTGGA GAGATCAGCT CCGCCCCTCC
    CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC AGGATCAAGG CCCCCGTGTA CCGGACAGAC
    CGAGTGACGT TTCAGGATAA GGACTACACC ATTGAGGAGA TAGAGGCTGG CAGACTCCCA
    AACCCACACC TGGGCCCAGT GGAGGAACGC TTAGCCCTGC ATGTCCTTCA GCAACAAGGC
    TTGGTTCCTG AGCATGTGGA GTCACGGCCT CTTTATAGTC CTCTGCAGCC AGATATCGAG
    CAGGGGAAGC TACAGATGTG GATTGACATA TTTCCAAAGG TGCTGGGCCG GCCTGGACCT
    CCCTTCAACA TCACCCCACG GAAAGCTAGA AGGTTTTTTC TGCGTTGTAT TATTTGGAAC
    ACAAAAGATG TGATCTTGGA TGACCTCAGT CTCACGGGGG AGAAGATGAG TGACATCTAT
    GTGAAAGGCT GGATGGTGGG ATTCGAAGAA CACAAGCAGA AGACAGATGT GCACTACCGC
    TCCCTGGGTG GGGAGGGCAA TTTCAACTGG AGGTTCGTGT TTCCCTTCGA CTATCTGCCT
    GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG GATGCCTTCT GGAGACTCGA CAAGACAGAG
    AGCAAGATCC CAGCACGTGT GGTCTTCCAG ATCTGGGACA ATGACAAATT CTCTTTTGAT
    GACTTTCTGG GCTCTCTGCA GCTGGATCTC AACCGAATGC CCAAGCCAGC CAAGACGGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACCTTCCACC CAGAATGGTT TGTGTCCCTT
    TTTGAGCAGA AGACAGTGAA AGGATGGTGG CCTTGTGTGA CAGAGGAGGG CGAGAAGAAG
    ATGTTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTTG CAGAAAGTGA ACATGAAGAG
    CGGCCTGCTG GCCAAGGTCG GGATGAACCC AACATGAATC CGAAGCTAGA GGATCCAAGG
    CGCCCCGATA CTTCTTTCCT GTGGTTCACC TCCCCGTACA AGACCATGAA GTTCATCTTG
    TGGCGACGCT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT CCTGCTCTTC
    CTGGGTGTCT TTGTCTACGC CTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTT
    CGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506233.1
    CDS255..6620
    Translation

    Target ORF information:

    RefSeq Version XM_006506170.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506170.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGTGGACGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC
    TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCA
    GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCTTATCGAG GCAGGGTTCT GTTGTCCCTG
    GAGACCAAAC TGGTGGAACA CAGTGAGCAG AAGGTGGAAG ACCTTCCTGC TGATGATATC
    CTGCGTGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA
    GCCACAATGC TGCAGGATGT GGATGACGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC
    GGGAACAAGT TCGACACCAC CTGCCTGCCC TTGGCCTCCA CCACCCAGTA CAGCCGGGCG
    GTCTTTGATG GATGCCACTA CTATTACTTG CCTTGGGGCA ACGTGAAGCC CGTGGTGGTG
    CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAATTGAGA TCCAAAACCA GCTCCTCAGG
    GTCGCTGACC GCCTGGAAGC TAACCTGGAG CAGGTCCACT TGGCTCTGAA GGCACAGTGT
    TCCTCCGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC
    AGCCAGCCCC TGTGTGACAT CCACGAGATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG
    CTCCGGCTAC GCACCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCTCTGGC CTTGAAGCTT
    GGCCATAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT
    GCCCTGGCCG AGGAGCCCCA GAACAGCCTG CCGGACATCA TCATCTGGAT GCTGCAGGGT
    GACAAGCGTG TGGCCTACCA GCGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC
    CCCAGCTACT GCGGCAGGAA CTGTGGGAAG CTGCAGACCA TCTTTCTGAA ATATCCTATG
    GAAGGTATGC CTGGGGCCCG AATGCCCGTG CAGATTCGGA TCAAGCTCTG GTTCGGGCTC
    TCTGTGGATG AGAAGGAATT CAATCAGTTT GCTGAGGGAA AGCTCTCTGT CTTTGCCGAA
    ACGTATGAGA ACCAGACAAA GCTGGCACTG GTCGGGAACT GGGGCACAAC GGGCCTCACC
    TACCCCAAGT TTTCTGATGT CACAGGCAAG ATCAAACTAC CAAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGGCCTGGGC AGGAGATTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GATGCTGACG CTGGTCATCT GAGCTTCGTG GAGGAGGTGT TCGAGAACCA GACCCGGCTC
    CCTGGGGGCC AGTGGATTTA CATGAGTGAT AACTACACAG ATGTGAATGG GGAGAAGGTA
    CTTCCGAAGG ATGATATTGA ATGCCCGCTG GGCTGGAAGT GGGAAGATGA AGAATGGTCC
    ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA
    GACCGAAAGC CAAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA
    CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AAGCACTGAA GAGGCACAGG
    CAGGCAGAGG CCGAGGGCGA GGGCTGGGAG TATGCCTCTC TCTTCGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCG
    CTGGAGAAGA CGGGACCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCATGGTG
    GATGACAAGA GCGAAGACTC CATGTCTGTT TCTACCCTGA GCTTTGGCGT TAACAGACCC
    ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGCTGCTA CCTGTACCAG
    GCCCGAGACC TGCCAGCCAT GGACAAGGAC TCCTTCTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCATC AAAGCCAGAA GACAGTGGTG GAGAAGAACA CCCTGAATCC CACCTGGGAT
    CAAACCCTCA TCTTCTATGA GATTGAGATC TTCGGCGAGC CAGCCAGTAT CGCCGAGCAT
    CCGCCCTGCA TCGTGGTGGA GCTCTATGAC CACGACACCT ATGGTGCTGA CGAGTTCATG
    GGTCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG
    ACCCGGGGCA GCCAGCCTGC CGGCGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG
    AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCATG AAACTTCAAG GATTTTGGAT
    GAGACGGAGG ACACAGATTT GCCTTACCCA CCACCTCAAA GGGAGGCCAA CATCTACATG
    GTTCCCCAGA ACATTAAGCC AGCTCTCCAA CGTACTGCCA TTGAGATCCT GGCCTGGGGC
    CTGCGGAACA TGAAGAGCTA TCAGATGGCC AGCATCTCTT CCCCCAGCCT CGTGGTGGAG
    TGTGGTGGGC AGACAGTGCA GTCCTGTGTC ATCAGAAACC TTCGGAAGAA CCCCAACTTT
    GATGTCTGTA CTCTCTTCAT GGAAGTGATG TTACCCAGAG AGGACCTCTA CTGTCCACCT
    ATTGTGGTCA AGGTCATTGA TAATCGCCAG TTTGGCCGCC GGCCAGTTGT GGGTCAGTGT
    ACCATTCGCT CCCTGGAGAA CTTCCTGTGC GACCCATACT CAGCAGAGAG TCCATCCCCA
    CAGGGTGGCC CAGATGATGT GAGCTTACTC AGCCCCGGGG AAGATGTGCT GATTGACATC
    GATGACAAGG AGCCTCTCAT TCCTGTCCAG CTTACAGATG GGCTGTCAAG CTTGGGCCCC
    ACTAACCTGA CGCCTTCTCC ATCCAGTCCT CATGAGGAGG AGTTCATTGA CTGGTGGAGC
    AAATTCTTTG CTTCTGTCGG GGAGAGGGAA AAGTGTGGTT CCTACCTGGA GAAGGATTTT
    GACACCTTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCTTTTGG AGGCCTGTCC
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAGGACTC AGGAGGAGAC AGATGATCCA
    TCTGTCATCG GAGAATTTAA GGGTCTCTTC AAAATTTATC CCCTCCCAGA AGATCCAGCC
    ATCCCCATGC CCCCGAGACA ATTCCATCAG CTGGCTGCTC AGGGTCCCCA GGAATGCTTG
    GTCCGTATTT ATATCGTCCG AGCATTTGGC TTACAGCCCA AGGACCCCAA TGGCAAGTGT
    GACCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC
    CCTTGCACCC TGGAGCCTGT ATTCGGAAAG ATGTTTGAAC TCACCTGCAC TCTACCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTACGAT TATGACCTGC TCTCCAAGGA TGAGAAGATT
    GGGGAGACGG TCATAGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGG
    CTCCCACAGA CCTACTGTGT CTCTGGACCA AATCAGTGGA GAGATCAGCT CCGCCCCTCC
    CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC AGGATCAAGG CCCCCGTGTA CCGGACAGAC
    CGAGTGACGT TTCAGGATAA GGACTACACC ATTGAGGAGA TAGAGGCTGG CAGACTCCCA
    AACCCACACC TGGGCCCAGT GGAGGAACGC TTAGCCCTGC ATGTCCTTCA GCAACAAGGC
    TTGGTTCCTG AGCATGTGGA GTCACGGCCT CTTTATAGTC CTCTGCAGCC AGATATCGAG
    CAGGGGAAGC TACAGATGTG GATTGACATA TTTCCAAAGG TGCTGGGCCG GCCTGGACCT
    CCCTTCAACA TCACCCCACG GAAAGCTAGA AGGTTTTTTC TGCGTTGTAT TATTTGGAAC
    ACAAAAGATG TGATCTTGGA TGACCTCAGT CTCACGGGGG AGAAGATGAG TGACATCTAT
    GTGAAAGGCT GGATGGTGGG ATTCGAAGAA CACAAGCAGA AGACAGATGT GCACTACCGC
    TCCCTGGGTG GGGAGGGCAA TTTCAACTGG AGGTTCGTGT TTCCCTTCGA CTATCTGCCT
    GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG GATGCCTTCT GGAGACTCGA CAAGACAGAG
    AGCAAGATCC CAGCACGTGT GGTCTTCCAG ATCTGGGACA ATGACAAATT CTCTTTTGAT
    GACTTTCTGG GCTCTCTGCA GCTGGATCTC AACCGAATGC CCAAGCCAGC CAAGACGGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACCTTCCACC CAGAATGGTT TGTGTCCCTT
    TTTGAGCAGA AGACAGTGAA AGGATGGTGG CCTTGTGTGA CAGAGGAGGG CGAGAAGAAG
    ATGTTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTTG CAGAAAGTGA ACATGAAGAG
    CGGCCTGCTG GCCAAGGTCG GGATGAACCC AACATGAATC CGAAGCTAGA GGATCCAAGG
    CGCCCCGATA CTTCTTTCCT GTGGTTCACC TCCCCGTACA AGACCATGAA GTTCATCTTG
    TGGCGACGCT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT CCTGCTCTTC
    CTGGGTGTCT TTGTCTACGC CTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTT
    CGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37299
    Clone ID Related Accession (Same CDS sequence) XM_006506171.3 , XM_006506171.4
    Accession Version XM_006506171.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGT GGACGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC
    TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCAGAG
    CTCAACACAG GAAAGGGAGA AGGTGTGGCT TATCGAGGCA GGGTTCTGTT GTCCCTGGAG
    ACCAAACTGG TGGAACACAG TGAGCAGAAG GTGGAAGACC TTCCTGCTGA TGATATCCTG
    CGTGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC
    ACAATGCTGC AGGATGTGGA TGACGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGG
    AACAAGTTCG ACACCACCTG CCTGCCCTTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGATGGAT GCCACTACTA TTACTTGCCT TGGGGCAACG TGAAGCCCGT GGTGGTGCTG
    TCCTCATACT GGGAAGACAT CAGCCATCGA ATTGAGATCC AAAACCAGCT CCTCAGGGTC
    GCTGACCGCC TGGAAGCTAA CCTGGAGCAG GTCCACTTGG CTCTGAAGGC ACAGTGTTCC
    TCCGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC
    CAGCCCCTGT GTGACATCCA CGAGATACCT TCTGCCACCC ACCTTGATCA GTACCTGCTC
    CGGCTACGCA CCCGTCACCT GAGCCAGATC AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC
    CATAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC
    CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG GACATCATCA TCTGGATGCT GCAGGGTGAC
    AAGCGTGTGG CCTACCAGCG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC
    AGCTACTGCG GCAGGAACTG TGGGAAGCTG CAGACCATCT TTCTGAAATA TCCTATGGAA
    GGTATGCCTG GGGCCCGAAT GCCCGTGCAG ATTCGGATCA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAATTCAA TCAGTTTGCT GAGGGAAAGC TCTCTGTCTT TGCCGAAACG
    TATGAGAACC AGACAAAGCT GGCACTGGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGATGTCAC AGGCAAGATC AAACTACCAA AGGATAGCTT CCGCCCCTCA
    GCTGGCTGGG CCTGGGCAGG AGATTGGTTC GTGTGTCCAG AGAAGACCCT ACTCCATGAT
    GCTGACGCTG GTCATCTGAG CTTCGTGGAG GAGGTGTTCG AGAACCAGAC CCGGCTCCCT
    GGGGGCCAGT GGATTTACAT GAGTGATAAC TACACAGATG TGAATGGGGA GAAGGTACTT
    CCGAAGGATG ATATTGAATG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC
    CGAAAGCCAA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC
    TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAAG CACTGAAGAG GCACAGGCAG
    GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT GCCTCTCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACAGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG
    GAGAAGACGG GACCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CATGGTGGAT
    GACAAGAGCG AAGACTCCAT GTCTGTTTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA
    ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GCTGCTACCT GTACCAGGCC
    CGAGACCTGC CAGCCATGGA CAAGGACTCC TTCTCTGATC CCTACGCCAT CGTCTCCTTC
    CTGCATCAAA GCCAGAAGAC AGTGGTGGAG AAGAACACCC TGAATCCCAC CTGGGATCAA
    ACCCTCATCT TCTATGAGAT TGAGATCTTC GGCGAGCCAG CCAGTATCGC CGAGCATCCG
    CCCTGCATCG TGGTGGAGCT CTATGACCAC GACACCTATG GTGCTGACGA GTTCATGGGT
    CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC
    CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG
    CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCATGAAA CTTCAAGGAT TTTGGATGAG
    ACGGAGGACA CAGATTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TTAAGCCAGC TCTCCAACGT ACTGCCATTG AGATCCTGGC CTGGGGCCTG
    CGGAACATGA AGAGCTATCA GATGGCCAGC ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT
    GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAT
    GTCTGTACTC TCTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCACCTATT
    GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCCGGC CAGTTGTGGG TCAGTGTACC
    ATTCGCTCCC TGGAGAACTT CCTGTGCGAC CCATACTCAG CAGAGAGTCC ATCCCCACAG
    GGTGGCCCAG ATGATGTGAG CTTACTCAGC CCCGGGGAAG ATGTGCTGAT TGACATCGAT
    GACAAGGAGC CTCTCATTCC TGTCCAGCTT ACAGATGGGC TGTCAAGCTT GGGCCCCACT
    AACCTGACGC CTTCTCCATC CAGTCCTCAT GAGGAGGAGT TCATTGACTG GTGGAGCAAA
    TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC
    ACCTTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CTTTTGGAGG CCTGTCCGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AGGACTCAGG AGGAGACAGA TGATCCATCT
    GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC
    CCCATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC
    CGTATTTATA TCGTCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT
    TGCACCCTGG AGCCTGTATT CGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG
    AAGGACCTGA AGATCACACT CTACGATTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG
    GAGACGGTCA TAGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC
    CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG
    CTCCTCCACC TCTTCTGCCA GCAGCACAGG ATCAAGGCCC CCGTGTACCG GACAGACCGA
    GTGACGTTTC AGGATAAGGA CTACACCATT GAGGAGATAG AGGCTGGCAG ACTCCCAAAC
    CCACACCTGG GCCCAGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAGCA ACAAGGCTTG
    GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT TATAGTCCTC TGCAGCCAGA TATCGAGCAG
    GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC
    TTCAACATCA CCCCACGGAA AGCTAGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA
    AAAGATGTGA TCTTGGATGA CCTCAGTCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG
    AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAGCAGAAGA CAGATGTGCA CTACCGCTCC
    CTGGGTGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCTGCT
    GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCCTTCTGGA GACTCGACAA GACAGAGAGC
    AAGATCCCAG CACGTGTGGT CTTCCAGATC TGGGACAATG ACAAATTCTC TTTTGATGAC
    TTTCTGGGCT CTCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT
    GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGA GAAGAAGATG
    TTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTTGCAG AAAGTGAACA TGAAGAGCGG
    CCTGCTGGCC AAGGTCGGGA TGAACCCAAC ATGAATCCGA AGCTAGAGGA TCCAAGGCGC
    CCCGATACTT CTTTCCTGTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCTTGTGG
    CGACGCTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG
    GGTGTCTTTG TCTACGCCTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTTCGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506234.1
    CDS601..6963
    Translation

    Target ORF information:

    RefSeq Version XM_006506171.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506171.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGT GGACGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC
    TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCAGAG
    CTCAACACAG GAAAGGGAGA AGGTGTGGCT TATCGAGGCA GGGTTCTGTT GTCCCTGGAG
    ACCAAACTGG TGGAACACAG TGAGCAGAAG GTGGAAGACC TTCCTGCTGA TGATATCCTG
    CGTGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC
    ACAATGCTGC AGGATGTGGA TGACGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGG
    AACAAGTTCG ACACCACCTG CCTGCCCTTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC
    TTTGATGGAT GCCACTACTA TTACTTGCCT TGGGGCAACG TGAAGCCCGT GGTGGTGCTG
    TCCTCATACT GGGAAGACAT CAGCCATCGA ATTGAGATCC AAAACCAGCT CCTCAGGGTC
    GCTGACCGCC TGGAAGCTAA CCTGGAGCAG GTCCACTTGG CTCTGAAGGC ACAGTGTTCC
    TCCGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC
    CAGCCCCTGT GTGACATCCA CGAGATACCT TCTGCCACCC ACCTTGATCA GTACCTGCTC
    CGGCTACGCA CCCGTCACCT GAGCCAGATC AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC
    CATAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC
    CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG GACATCATCA TCTGGATGCT GCAGGGTGAC
    AAGCGTGTGG CCTACCAGCG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC
    AGCTACTGCG GCAGGAACTG TGGGAAGCTG CAGACCATCT TTCTGAAATA TCCTATGGAA
    GGTATGCCTG GGGCCCGAAT GCCCGTGCAG ATTCGGATCA AGCTCTGGTT CGGGCTCTCT
    GTGGATGAGA AGGAATTCAA TCAGTTTGCT GAGGGAAAGC TCTCTGTCTT TGCCGAAACG
    TATGAGAACC AGACAAAGCT GGCACTGGTC GGGAACTGGG GCACAACGGG CCTCACCTAC
    CCCAAGTTTT CTGATGTCAC AGGCAAGATC AAACTACCAA AGGATAGCTT CCGCCCCTCA
    GCTGGCTGGG CCTGGGCAGG AGATTGGTTC GTGTGTCCAG AGAAGACCCT ACTCCATGAT
    GCTGACGCTG GTCATCTGAG CTTCGTGGAG GAGGTGTTCG AGAACCAGAC CCGGCTCCCT
    GGGGGCCAGT GGATTTACAT GAGTGATAAC TACACAGATG TGAATGGGGA GAAGGTACTT
    CCGAAGGATG ATATTGAATG CCCGCTGGGC TGGAAGTGGG AAGATGAAGA ATGGTCCACA
    GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC
    CGAAAGCCAA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC
    TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAAG CACTGAAGAG GCACAGGCAG
    GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT GCCTCTCTCT TCGGCTGGAA GTTCCACCTG
    GAGTACCGCA AGACAGATGC CTTCCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG
    GAGAAGACGG GACCTGCAGC TGTGTTTGCC CTCGAGGGGG CCCTGGGCGG CATGGTGGAT
    GACAAGAGCG AAGACTCCAT GTCTGTTTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA
    ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GCTGCTACCT GTACCAGGCC
    CGAGACCTGC CAGCCATGGA CAAGGACTCC TTCTCTGATC CCTACGCCAT CGTCTCCTTC
    CTGCATCAAA GCCAGAAGAC AGTGGTGGAG AAGAACACCC TGAATCCCAC CTGGGATCAA
    ACCCTCATCT TCTATGAGAT TGAGATCTTC GGCGAGCCAG CCAGTATCGC CGAGCATCCG
    CCCTGCATCG TGGTGGAGCT CTATGACCAC GACACCTATG GTGCTGACGA GTTCATGGGT
    CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC
    CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG
    CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCATGAAA CTTCAAGGAT TTTGGATGAG
    ACGGAGGACA CAGATTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT
    CCCCAGAACA TTAAGCCAGC TCTCCAACGT ACTGCCATTG AGATCCTGGC CTGGGGCCTG
    CGGAACATGA AGAGCTATCA GATGGCCAGC ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT
    GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAT
    GTCTGTACTC TCTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCACCTATT
    GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCCGGC CAGTTGTGGG TCAGTGTACC
    ATTCGCTCCC TGGAGAACTT CCTGTGCGAC CCATACTCAG CAGAGAGTCC ATCCCCACAG
    GGTGGCCCAG ATGATGTGAG CTTACTCAGC CCCGGGGAAG ATGTGCTGAT TGACATCGAT
    GACAAGGAGC CTCTCATTCC TGTCCAGCTT ACAGATGGGC TGTCAAGCTT GGGCCCCACT
    AACCTGACGC CTTCTCCATC CAGTCCTCAT GAGGAGGAGT TCATTGACTG GTGGAGCAAA
    TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC
    ACCTTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CTTTTGGAGG CCTGTCCGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AGGACTCAGG AGGAGACAGA TGATCCATCT
    GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC
    CCCATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC
    CGTATTTATA TCGTCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGTGAC
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT
    TGCACCCTGG AGCCTGTATT CGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG
    AAGGACCTGA AGATCACACT CTACGATTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG
    GAGACGGTCA TAGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC
    CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG
    CTCCTCCACC TCTTCTGCCA GCAGCACAGG ATCAAGGCCC CCGTGTACCG GACAGACCGA
    GTGACGTTTC AGGATAAGGA CTACACCATT GAGGAGATAG AGGCTGGCAG ACTCCCAAAC
    CCACACCTGG GCCCAGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAGCA ACAAGGCTTG
    GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT TATAGTCCTC TGCAGCCAGA TATCGAGCAG
    GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC
    TTCAACATCA CCCCACGGAA AGCTAGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA
    AAAGATGTGA TCTTGGATGA CCTCAGTCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG
    AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAGCAGAAGA CAGATGTGCA CTACCGCTCC
    CTGGGTGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCTGCT
    GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCCTTCTGGA GACTCGACAA GACAGAGAGC
    AAGATCCCAG CACGTGTGGT CTTCCAGATC TGGGACAATG ACAAATTCTC TTTTGATGAC
    TTTCTGGGCT CTCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT
    GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGA GAAGAAGATG
    TTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTTGCAG AAAGTGAACA TGAAGAGCGG
    CCTGCTGGCC AAGGTCGGGA TGAACCCAAC ATGAATCCGA AGCTAGAGGA TCCAAGGCGC
    CCCGATACTT CTTTCCTGTG GTTCACCTCC CCGTACAAGA CCATGAAGTT CATCTTGTGG
    CGACGCTTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG
    GGTGTCTTTG TCTACGCCTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTTCGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37300
    Clone ID Related Accession (Same CDS sequence) XM_006506172.2
    Accession Version XM_006506172.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGACGGA GGACACAGAT
    TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATTAAG
    CCAGCTCTCC AACGTACTGC CATTGAGATC CTGGCCTGGG GCCTGCGGAA CATGAAGAGC
    TATCAGATGG CCAGCATCTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG
    CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGATGTCTG TACTCTCTTC
    ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCAC CTATTGTGGT CAAGGTCATT
    GATAATCGCC AGTTTGGCCG CCGGCCAGTT GTGGGTCAGT GTACCATTCG CTCCCTGGAG
    AACTTCCTGT GCGACCCATA CTCAGCAGAG AGTCCATCCC CACAGGGTGG CCCAGATGAT
    GTGAGCTTAC TCAGCCCCGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCTCTC
    ATTCCTGTCC AGGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTGTCGGG
    GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCTTGAA GGTCTATGAC
    ACACAGCTGG AGAATGTGGA GGCTTTTGGA GGCCTGTCCG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAGGACTCA GGAGGAGACA GATGATCCAT CTGTCATCGG AGAATTTAAG
    GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCCATGCC CCCGAGACAA
    TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGTATTTA TATCGTCCGA
    GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGCACCCT GGAGCCTGTA
    TTCGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACCT GAAGATCACA
    CTCTACGATT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACGGT CATAGACCTG
    GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGGC TCCCACAGAC CTACTGTGTC
    TCTGGACCAA ATCAGTGGAG AGATCAGCTC CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC
    CAGCAGCACA GGATCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG
    GACTACACCA TTGAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCAGTG
    GAGGAACGCT TAGCCCTGCA TGTCCTTCAG CAACAAGGCT TGGTTCCTGA GCATGTGGAG
    TCACGGCCTC TTTATAGTCC TCTGCAGCCA GATATCGAGC AGGGGAAGCT ACAGATGTGG
    ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG
    AAAGCTAGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT
    GACCTCAGTC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA
    TTCGAAGAAC ACAAGCAGAA GACAGATGTG CACTACCGCT CCCTGGGTGG GGAGGGCAAT
    TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCTG CTGAGCAAGT CTGTGCTGTT
    GCCAAGAAGG ATGCCTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG
    GTCTTCCAGA TCTGGGACAA TGACAAATTC TCTTTTGATG ACTTTCTGGG CTCTCTGCAG
    CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA
    GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA
    ATGACCTTGG AGATTGTTGC AGAAAGTGAA CATGAAGAGC GGCCTGCTGG CCAAGGTCGG
    GATGAACCCA ACATGAATCC GAAGCTAGAG GATCCAAGGC GCCCCGATAC TTCTTTCCTG
    TGGTTCACCT CCCCGTACAA GACCATGAAG TTCATCTTGT GGCGACGCTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506235.1
    CDS255..6599
    Translation

    Target ORF information:

    RefSeq Version XM_006506172.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506172.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG GAAGAAGTGG
    CCGGTCCCCA CGGACACAGG AGGGGAGGAA GACACCGAGG ACCAGGGCCT GACTGGAGAT
    GAAGCTGAGC CGTTCCTGGA CCAGAGTGCT GCTGTGGGCC CAGGGGGACC GACCACACCC
    AGGAAGCCAC CTTCCCACCC TCCTCCCCAC TATCCAGGGG CAAAAAGAAA AAGAAGCTCT
    GCCCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG
    ATAGAGGGGC GTCAGCTGCC TGGGGTGAAT ATTAAGCCTG TGGTCAAGGT CACAGCTGCT
    GGGCAGACCA AGCGAACTCG GATCCAGAAG GGAAATAGCC CACTCTTCAA TGAGACTCTT
    TTCTTCAACG TATTTGACTC TCCCTTGGAG CTGTTTGATG AGCCCATCTT CATCACGGTG
    GTGGACTCAC GCTCCCTTAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC
    ACCGTGTACA GAGAGCCCCG ACACGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGACCCT
    GATGACTTCT CTGCGGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTAGGGCCT
    GGAGATGAAG CTCCCCTGGA CAAGAAGGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC
    AACCTGCTTA GGCCCACCGG TGTGGCCCTT CGAGGAGCCC ACTTCTGCCT GAAGCTCTTC
    CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAACTTGGTG GATCCCTTTG TGGAAGTCAG CTTTGCTGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCTAACC CTCAGTGGAA CCAGAACATC
    ACCTTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG
    GACCGCCTCA CTCACAATGA CACTGTGGCC ACCACCTACT TGGGTATGTC GAAAATCTCT
    GCTACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC ACAAGCCACT
    GACCTGGACG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT
    GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CAGAGCTCAA CACAGGAAAG
    GGAGAAGGTG TGGCTTATCG AGGCAGGGTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAA
    CACAGTGAGC AGAAGGTGGA AGACCTTCCT GCTGATGATA TCCTGCGTGT GGAGAAGTAC
    CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT
    GTGGATGACG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGGAACAA GTTCGACACC
    ACCTGCCTGC CCTTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA TGGATGCCAC
    TACTATTACT TGCCTTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCCTC ATACTGGGAA
    GACATCAGCC ATCGAATTGA GATCCAAAAC CAGCTCCTCA GGGTCGCTGA CCGCCTGGAA
    GCTAACCTGG AGCAGGTCCA CTTGGCTCTG AAGGCACAGT GTTCCTCCGA GGATGTGGAC
    GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC
    ATCCACGAGA TACCTTCTGC CACCCACCTT GATCAGTACC TGCTCCGGCT ACGCACCCGT
    CACCTGAGCC AGATCAAAGA AGCTGCTCTG GCCTTGAAGC TTGGCCATAG TGAGCTCTCC
    ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC CGAGGAGCCC
    CAGAACAGCC TGCCGGACAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC
    CAGCGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG
    AACTGTGGGA AGCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGTAT GCCTGGGGCC
    CGAATGCCCG TGCAGATTCG GATCAAGCTC TGGTTCGGGC TCTCTGTGGA TGAGAAGGAA
    TTCAATCAGT TTGCTGAGGG AAAGCTCTCT GTCTTTGCCG AAACGTATGA GAACCAGACA
    AAGCTGGCAC TGGTCGGGAA CTGGGGCACA ACGGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCACAGGCA AGATCAAACT ACCAAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG
    GCAGGAGATT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGATGCTGA CGCTGGTCAT
    CTGAGCTTCG TGGAGGAGGT GTTCGAGAAC CAGACCCGGC TCCCTGGGGG CCAGTGGATT
    TACATGAGTG ATAACTACAC AGATGTGAAT GGGGAGAAGG TACTTCCGAA GGATGATATT
    GAATGCCCGC TGGGCTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT
    GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCAAAACAC
    TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC
    AGGAGGGACC TCAGCCAGAT GGAAGCACTG AAGAGGCACA GGCAGGCAGA GGCCGAGGGC
    GAGGGCTGGG AGTATGCCTC TCTCTTCGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA
    GATGCCTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CGCTGGAGAA GACGGGACCT
    GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAC
    TCCATGTCTG TTTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT
    GACTATGGGA ACCGCTACCA TCTGCGCTGC TACCTGTACC AGGCCCGAGA CCTGCCAGCC
    ATGGACAAGG ACTCCTTCTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA TCAAAGCCAG
    AAGACAGTGG TGGAGAAGAA CACCCTGAAT CCCACCTGGG ATCAAACCCT CATCTTCTAT
    GAGATTGAGA TCTTCGGCGA GCCAGCCAGT ATCGCCGAGC ATCCGCCCTG CATCGTGGTG
    GAGCTCTATG ACCACGACAC CTATGGTGCT GACGAGTTCA TGGGTCGCTG TATTTGTCAG
    CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCCGGGG CAGCCAGCCT
    GCCGGCGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC
    ATCCCTGGCT TTGAGATGCA TGAAACTTCA AGGATTTTGG ATGAGACGGA GGACACAGAT
    TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATTAAG
    CCAGCTCTCC AACGTACTGC CATTGAGATC CTGGCCTGGG GCCTGCGGAA CATGAAGAGC
    TATCAGATGG CCAGCATCTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG
    CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGATGTCTG TACTCTCTTC
    ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCAC CTATTGTGGT CAAGGTCATT
    GATAATCGCC AGTTTGGCCG CCGGCCAGTT GTGGGTCAGT GTACCATTCG CTCCCTGGAG
    AACTTCCTGT GCGACCCATA CTCAGCAGAG AGTCCATCCC CACAGGGTGG CCCAGATGAT
    GTGAGCTTAC TCAGCCCCGG GGAAGATGTG CTGATTGACA TCGATGACAA GGAGCCTCTC
    ATTCCTGTCC AGGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTGTCGGG
    GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCTTGAA GGTCTATGAC
    ACACAGCTGG AGAATGTGGA GGCTTTTGGA GGCCTGTCCG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAGGACTCA GGAGGAGACA GATGATCCAT CTGTCATCGG AGAATTTAAG
    GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCCATGCC CCCGAGACAA
    TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGTATTTA TATCGTCCGA
    GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGTG ACCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGCACCCT GGAGCCTGTA
    TTCGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACCT GAAGATCACA
    CTCTACGATT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACGGT CATAGACCTG
    GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGGC TCCCACAGAC CTACTGTGTC
    TCTGGACCAA ATCAGTGGAG AGATCAGCTC CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC
    CAGCAGCACA GGATCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG
    GACTACACCA TTGAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCAGTG
    GAGGAACGCT TAGCCCTGCA TGTCCTTCAG CAACAAGGCT TGGTTCCTGA GCATGTGGAG
    TCACGGCCTC TTTATAGTCC TCTGCAGCCA GATATCGAGC AGGGGAAGCT ACAGATGTGG
    ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG
    AAAGCTAGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT
    GACCTCAGTC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA
    TTCGAAGAAC ACAAGCAGAA GACAGATGTG CACTACCGCT CCCTGGGTGG GGAGGGCAAT
    TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCTG CTGAGCAAGT CTGTGCTGTT
    GCCAAGAAGG ATGCCTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG
    GTCTTCCAGA TCTGGGACAA TGACAAATTC TCTTTTGATG ACTTTCTGGG CTCTCTGCAG
    CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA
    GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA
    ATGACCTTGG AGATTGTTGC AGAAAGTGAA CATGAAGAGC GGCCTGCTGG CCAAGGTCGG
    GATGAACCCA ACATGAATCC GAAGCTAGAG GATCCAAGGC GCCCCGATAC TTCTTTCCTG
    TGGTTCACCT CCCCGTACAA GACCATGAAG TTCATCTTGT GGCGACGCTT CCGGTGTGCC
    ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37301
    Clone ID Related Accession (Same CDS sequence) XM_006506173.4 , XM_006506173.3
    Accession Version XM_006506173.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCACA AGCCACTGAC
    CTGGACGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC
    AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCAG AGCTCAACAC AGGAAAGGGA
    GAAGGTGTGG CTTATCGAGG CAGGGTTCTG TTGTCCCTGG AGACCAAACT GGTGGAACAC
    AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT GATGATATCC TGCGTGTGGA GAAGTACCTG
    CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG
    GATGACGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GGAACAAGTT CGACACCACC
    TGCCTGCCCT TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC
    TATTACTTGC CTTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCCTCATA CTGGGAAGAC
    ATCAGCCATC GAATTGAGAT CCAAAACCAG CTCCTCAGGG TCGCTGACCG CCTGGAAGCT
    AACCTGGAGC AGGTCCACTT GGCTCTGAAG GCACAGTGTT CCTCCGAGGA TGTGGACGCC
    CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC
    CACGAGATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGC TCCGGCTACG CACCCGTCAC
    CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC TTGAAGCTTG GCCATAGTGA GCTCTCCACT
    GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCCGA GGAGCCCCAG
    AACAGCCTGC CGGACATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAG
    CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC
    TGTGGGAAGC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGTATGCC TGGGGCCCGA
    ATGCCCGTGC AGATTCGGAT CAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAATTC
    AATCAGTTTG CTGAGGGAAA GCTCTCTGTC TTTGCCGAAA CGTATGAGAA CCAGACAAAG
    CTGGCACTGG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ACAGGCAAGA TCAAACTACC AAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA
    GGAGATTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ATGCTGACGC TGGTCATCTG
    AGCTTCGTGG AGGAGGTGTT CGAGAACCAG ACCCGGCTCC CTGGGGGCCA GTGGATTTAC
    ATGAGTGATA ACTACACAGA TGTGAATGGG GAGAAGGTAC TTCCGAAGGA TGATATTGAA
    TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT
    GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC AAAACACTGG
    GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG
    AGGGACCTCA GCCAGATGGA AGCACTGAAG AGGCACAGGC AGGCAGAGGC CGAGGGCGAG
    GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGACCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGACTCC
    ATGTCTGTTT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC
    TATGGGAACC GCTACCATCT GCGCTGCTAC CTGTACCAGG CCCGAGACCT GCCAGCCATG
    GACAAGGACT CCTTCTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCATCA AAGCCAGAAG
    ACAGTGGTGG AGAAGAACAC CCTGAATCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG
    ATTGAGATCT TCGGCGAGCC AGCCAGTATC GCCGAGCATC CGCCCTGCAT CGTGGTGGAG
    CTCTATGACC ACGACACCTA TGGTGCTGAC GAGTTCATGG GTCGCTGTAT TTGTCAGCCA
    AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCCGGGGCAG CCAGCCTGCC
    GGCGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC
    CCTGGCTTTG AGATGCATGA AACTTCAAGG ATTTTGGATG AGACGGAGGA CACAGATTTG
    CCTTACCCAC CACCTCAAAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATTAAGCCA
    GCTCTCCAAC GTACTGCCAT TGAGATCCTG GCCTGGGGCC TGCGGAACAT GAAGAGCTAT
    CAGATGGCCA GCATCTCTTC CCCCAGCCTC GTGGTGGAGT GTGGTGGGCA GACAGTGCAG
    TCCTGTGTCA TCAGAAACCT TCGGAAGAAC CCCAACTTTG ATGTCTGTAC TCTCTTCATG
    GAAGTGATGT TACCCAGAGA GGACCTCTAC TGTCCACCTA TTGTGGTCAA GGTCATTGAT
    AATCGCCAGT TTGGCCGCCG GCCAGTTGTG GGTCAGTGTA CCATTCGCTC CCTGGAGAAC
    TTCCTGTGCG ACCCATACTC AGCAGAGAGT CCATCCCCAC AGGGTGGCCC AGATGATGTG
    AGCTTACTCA GCCCCGGGGA AGATGTGCTG ATTGACATCG ATGACAAGGA GCCTCTCATT
    CCTGTCCAGG AGGAGGAGTT CATTGACTGG TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG
    AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG GATTTTGACA CCTTGAAGGT CTATGACACA
    CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC CTGTCCGACT TTTGTAACAC CTTCAAGCTC
    TACCGGGGCA GGACTCAGGA GGAGACAGAT GATCCATCTG TCATCGGAGA ATTTAAGGGT
    CTCTTCAAAA TTTATCCCCT CCCAGAAGAT CCAGCCATCC CCATGCCCCC GAGACAATTC
    CATCAGCTGG CTGCTCAGGG TCCCCAGGAA TGCTTGGTCC GTATTTATAT CGTCCGAGCA
    TTTGGCTTAC AGCCCAAGGA CCCCAATGGC AAGTGTGACC CTTACATCAA GATCTCCATA
    GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCTT GCACCCTGGA GCCTGTATTC
    GGAAAGATGT TTGAACTCAC CTGCACTCTA CCTCTGGAGA AGGACCTGAA GATCACACTC
    TACGATTATG ACCTGCTCTC CAAGGATGAG AAGATTGGGG AGACGGTCAT AGACCTGGAG
    AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGGCTCC CACAGACCTA CTGTGTCTCT
    GGACCAAATC AGTGGAGAGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG
    CAGCACAGGA TCAAGGCCCC CGTGTACCGG ACAGACCGAG TGACGTTTCA GGATAAGGAC
    TACACCATTG AGGAGATAGA GGCTGGCAGA CTCCCAAACC CACACCTGGG CCCAGTGGAG
    GAACGCTTAG CCCTGCATGT CCTTCAGCAA CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA
    CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT ATCGAGCAGG GGAAGCTACA GATGTGGATT
    GACATATTTC CAAAGGTGCT GGGCCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAAA
    GCTAGAAGGT TTTTTCTGCG TTGTATTATT TGGAACACAA AAGATGTGAT CTTGGATGAC
    CTCAGTCTCA CGGGGGAGAA GATGAGTGAC ATCTATGTGA AAGGCTGGAT GGTGGGATTC
    GAAGAACACA AGCAGAAGAC AGATGTGCAC TACCGCTCCC TGGGTGGGGA GGGCAATTTC
    AACTGGAGGT TCGTGTTTCC CTTCGACTAT CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC
    AAGAAGGATG CCTTCTGGAG ACTCGACAAG ACAGAGAGCA AGATCCCAGC ACGTGTGGTC
    TTCCAGATCT GGGACAATGA CAAATTCTCT TTTGATGACT TTCTGGGCTC TCTGCAGCTG
    GATCTCAACC GAATGCCCAA GCCAGCCAAG ACGGCTGAGA AGTGCTCCTT GGACCAGCTG
    GATGACACCT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA
    TGGTGGCCTT GTGTGACAGA GGAGGGCGAG AAGAAGATGT TGGCGGGCAA GCTGGAAATG
    ACCTTGGAGA TTGTTGCAGA AAGTGAACAT GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT
    GAACCCAACA TGAATCCGAA GCTAGAGGAT CCAAGGCGCC CCGATACTTC TTTCCTGTGG
    TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCTTGTGGC GACGCTTCCG GTGTGCCATC
    ATCCTCTTCA TCATCCTCTT CATCCTCCTG CTCTTCCTGG GTGTCTTTGT CTACGCCTTC
    CCGAACTACG CTGCCATGAA GCTGGTGAAG CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506236.1
    CDS601..6942
    Translation

    Target ORF information:

    RefSeq Version XM_006506173.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506173.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGGAA GAAGTGGCCG
    GTCCCCACGG ACACAGGAGG GGAGGAAGAC ACCGAGGACC AGGGCCTGAC TGGAGATGAA
    GCTGAGCCGT TCCTGGACCA GAGTGCTGCT GTGGGCCCAG GGGGACCGAC CACACCCAGG
    AAGCCACCTT CCCACCCTCC TCCCCACTAT CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC
    CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATA
    GAGGGGCGTC AGCTGCCTGG GGTGAATATT AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG
    CAGACCAAGC GAACTCGGAT CCAGAAGGGA AATAGCCCAC TCTTCAATGA GACTCTTTTC
    TTCAACGTAT TTGACTCTCC CTTGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTG
    GACTCACGCT CCCTTAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC
    GTGTACAGAG AGCCCCGACA CGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGACCCTGAT
    GACTTCTCTG CGGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA
    GATGAAGCTC CCCTGGACAA GAAGGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC
    CTGCTTAGGC CCACCGGTGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GCTCTTCCGG
    GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA CTTGGTGGAT CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCTAACCCTC AGTGGAACCA GAACATCACC
    TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC
    CGCCTCACTC ACAATGACAC TGTGGCCACC ACCTACTTGG GTATGTCGAA AATCTCTGCT
    ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCACA AGCCACTGAC
    CTGGACGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC
    AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCAG AGCTCAACAC AGGAAAGGGA
    GAAGGTGTGG CTTATCGAGG CAGGGTTCTG TTGTCCCTGG AGACCAAACT GGTGGAACAC
    AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT GATGATATCC TGCGTGTGGA GAAGTACCTG
    CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG
    GATGACGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GGAACAAGTT CGACACCACC
    TGCCTGCCCT TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGATGG ATGCCACTAC
    TATTACTTGC CTTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCCTCATA CTGGGAAGAC
    ATCAGCCATC GAATTGAGAT CCAAAACCAG CTCCTCAGGG TCGCTGACCG CCTGGAAGCT
    AACCTGGAGC AGGTCCACTT GGCTCTGAAG GCACAGTGTT CCTCCGAGGA TGTGGACGCC
    CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC
    CACGAGATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGC TCCGGCTACG CACCCGTCAC
    CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC TTGAAGCTTG GCCATAGTGA GCTCTCCACT
    GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCCGA GGAGCCCCAG
    AACAGCCTGC CGGACATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAG
    CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC
    TGTGGGAAGC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGTATGCC TGGGGCCCGA
    ATGCCCGTGC AGATTCGGAT CAAGCTCTGG TTCGGGCTCT CTGTGGATGA GAAGGAATTC
    AATCAGTTTG CTGAGGGAAA GCTCTCTGTC TTTGCCGAAA CGTATGAGAA CCAGACAAAG
    CTGGCACTGG TCGGGAACTG GGGCACAACG GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ACAGGCAAGA TCAAACTACC AAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA
    GGAGATTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ATGCTGACGC TGGTCATCTG
    AGCTTCGTGG AGGAGGTGTT CGAGAACCAG ACCCGGCTCC CTGGGGGCCA GTGGATTTAC
    ATGAGTGATA ACTACACAGA TGTGAATGGG GAGAAGGTAC TTCCGAAGGA TGATATTGAA
    TGCCCGCTGG GCTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT
    GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC AAAACACTGG
    GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG
    AGGGACCTCA GCCAGATGGA AGCACTGAAG AGGCACAGGC AGGCAGAGGC CGAGGGCGAG
    GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT
    GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCGC TGGAGAAGAC GGGACCTGCA
    GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGACTCC
    ATGTCTGTTT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC
    TATGGGAACC GCTACCATCT GCGCTGCTAC CTGTACCAGG CCCGAGACCT GCCAGCCATG
    GACAAGGACT CCTTCTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCATCA AAGCCAGAAG
    ACAGTGGTGG AGAAGAACAC CCTGAATCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG
    ATTGAGATCT TCGGCGAGCC AGCCAGTATC GCCGAGCATC CGCCCTGCAT CGTGGTGGAG
    CTCTATGACC ACGACACCTA TGGTGCTGAC GAGTTCATGG GTCGCTGTAT TTGTCAGCCA
    AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCCGGGGCAG CCAGCCTGCC
    GGCGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC
    CCTGGCTTTG AGATGCATGA AACTTCAAGG ATTTTGGATG AGACGGAGGA CACAGATTTG
    CCTTACCCAC CACCTCAAAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATTAAGCCA
    GCTCTCCAAC GTACTGCCAT TGAGATCCTG GCCTGGGGCC TGCGGAACAT GAAGAGCTAT
    CAGATGGCCA GCATCTCTTC CCCCAGCCTC GTGGTGGAGT GTGGTGGGCA GACAGTGCAG
    TCCTGTGTCA TCAGAAACCT TCGGAAGAAC CCCAACTTTG ATGTCTGTAC TCTCTTCATG
    GAAGTGATGT TACCCAGAGA GGACCTCTAC TGTCCACCTA TTGTGGTCAA GGTCATTGAT
    AATCGCCAGT TTGGCCGCCG GCCAGTTGTG GGTCAGTGTA CCATTCGCTC CCTGGAGAAC
    TTCCTGTGCG ACCCATACTC AGCAGAGAGT CCATCCCCAC AGGGTGGCCC AGATGATGTG
    AGCTTACTCA GCCCCGGGGA AGATGTGCTG ATTGACATCG ATGACAAGGA GCCTCTCATT
    CCTGTCCAGG AGGAGGAGTT CATTGACTGG TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG
    AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG GATTTTGACA CCTTGAAGGT CTATGACACA
    CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC CTGTCCGACT TTTGTAACAC CTTCAAGCTC
    TACCGGGGCA GGACTCAGGA GGAGACAGAT GATCCATCTG TCATCGGAGA ATTTAAGGGT
    CTCTTCAAAA TTTATCCCCT CCCAGAAGAT CCAGCCATCC CCATGCCCCC GAGACAATTC
    CATCAGCTGG CTGCTCAGGG TCCCCAGGAA TGCTTGGTCC GTATTTATAT CGTCCGAGCA
    TTTGGCTTAC AGCCCAAGGA CCCCAATGGC AAGTGTGACC CTTACATCAA GATCTCCATA
    GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCTT GCACCCTGGA GCCTGTATTC
    GGAAAGATGT TTGAACTCAC CTGCACTCTA CCTCTGGAGA AGGACCTGAA GATCACACTC
    TACGATTATG ACCTGCTCTC CAAGGATGAG AAGATTGGGG AGACGGTCAT AGACCTGGAG
    AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGGCTCC CACAGACCTA CTGTGTCTCT
    GGACCAAATC AGTGGAGAGA TCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG
    CAGCACAGGA TCAAGGCCCC CGTGTACCGG ACAGACCGAG TGACGTTTCA GGATAAGGAC
    TACACCATTG AGGAGATAGA GGCTGGCAGA CTCCCAAACC CACACCTGGG CCCAGTGGAG
    GAACGCTTAG CCCTGCATGT CCTTCAGCAA CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA
    CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT ATCGAGCAGG GGAAGCTACA GATGTGGATT
    GACATATTTC CAAAGGTGCT GGGCCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAAA
    GCTAGAAGGT TTTTTCTGCG TTGTATTATT TGGAACACAA AAGATGTGAT CTTGGATGAC
    CTCAGTCTCA CGGGGGAGAA GATGAGTGAC ATCTATGTGA AAGGCTGGAT GGTGGGATTC
    GAAGAACACA AGCAGAAGAC AGATGTGCAC TACCGCTCCC TGGGTGGGGA GGGCAATTTC
    AACTGGAGGT TCGTGTTTCC CTTCGACTAT CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC
    AAGAAGGATG CCTTCTGGAG ACTCGACAAG ACAGAGAGCA AGATCCCAGC ACGTGTGGTC
    TTCCAGATCT GGGACAATGA CAAATTCTCT TTTGATGACT TTCTGGGCTC TCTGCAGCTG
    GATCTCAACC GAATGCCCAA GCCAGCCAAG ACGGCTGAGA AGTGCTCCTT GGACCAGCTG
    GATGACACCT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA
    TGGTGGCCTT GTGTGACAGA GGAGGGCGAG AAGAAGATGT TGGCGGGCAA GCTGGAAATG
    ACCTTGGAGA TTGTTGCAGA AAGTGAACAT GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT
    GAACCCAACA TGAATCCGAA GCTAGAGGAT CCAAGGCGCC CCGATACTTC TTTCCTGTGG
    TTCACCTCCC CGTACAAGAC CATGAAGTTC ATCTTGTGGC GACGCTTCCG GTGTGCCATC
    ATCCTCTTCA TCATCCTCTT CATCCTCCTG CTCTTCCTGG GTGTCTTTGT CTACGCCTTC
    CCGAACTACG CTGCCATGAA GCTGGTGAAG CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37302
    Clone ID Related Accession (Same CDS sequence) XM_006506174.2
    Accession Version XM_006506174.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTACTCA AGAGTCCACA AGCCACTGAC CTGGACGACA ACCTGGGCTT CCTCCCCACT
    TTTGGGCCCT GCTATGTGAA CCTCTATGGC AGTCCCCGAG AGTTCACGGG CTTCCCAGAT
    CCTTATGCAG AGCTCAACAC AGGAAAGGGA GAAGGTGTGG CTTATCGAGG CAGGGTTCTG
    TTGTCCCTGG AGACCAAACT GGTGGAACAC AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT
    GATGATATCC TGCGTGTGGA GAAGTACCTG CGGAGGCGCA AATATTCCCT CTTTGCTGCC
    TTCTACTCAG CCACAATGCT GCAGGATGTG GATGACGCCA TCCAGTTCGA GGTCAGCATT
    GGGAACTACG GGAACAAGTT CGACACCACC TGCCTGCCCT TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGATGG ATGCCACTAC TATTACTTGC CTTGGGGCAA CGTGAAGCCC
    GTGGTGGTGC TGTCCTCATA CTGGGAAGAC ATCAGCCATC GAATTGAGAT CCAAAACCAG
    CTCCTCAGGG TCGCTGACCG CCTGGAAGCT AACCTGGAGC AGGTCCACTT GGCTCTGAAG
    GCACAGTGTT CCTCCGAGGA TGTGGACGCC CTGGTGGCTC AGTTGACAGA TGAGCTCCTT
    GCAGACTGCA GCCAGCCCCT GTGTGACATC CACGAGATAC CTTCTGCCAC CCACCTTGAT
    CAGTACCTGC TCCGGCTACG CACCCGTCAC CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC
    TTGAAGCTTG GCCATAGTGA GCTCTCCACT GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG
    CATCTCCGTG CCCTGGCCGA GGAGCCCCAG AACAGCCTGC CGGACATCAT CATCTGGATG
    CTGCAGGGTG ACAAGCGTGT GGCCTACCAG CGGGTGCCTG CCCATGAAGT CCTCTTCTCC
    CGGCGGGGCC CCAGCTACTG CGGCAGGAAC TGTGGGAAGC TGCAGACCAT CTTTCTGAAA
    TATCCTATGG AAGGTATGCC TGGGGCCCGA ATGCCCGTGC AGATTCGGAT CAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAATTC AATCAGTTTG CTGAGGGAAA GCTCTCTGTC
    TTTGCCGAAA CGTATGAGAA CCAGACAAAG CTGGCACTGG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACAGGCAAGA TCAAACTACC AAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA GGAGATTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ATGCTGACGC TGGTCATCTG AGCTTCGTGG AGGAGGTGTT CGAGAACCAG
    ACCCGGCTCC CTGGGGGCCA GTGGATTTAC ATGAGTGATA ACTACACAGA TGTGAATGGG
    GAGAAGGTAC TTCCGAAGGA TGATATTGAA TGCCCGCTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCTGTT GATGAACAAG GCTGGGAGTA CAGCATCACC
    ATACCCCCAG ACCGAAAGCC AAAACACTGG GTCCCTGTTG AGAAGATGTA TTACACTCAC
    AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG AGGGACCTCA GCCAGATGGA AGCACTGAAG
    AGGCACAGGC AGGCAGAGGC CGAGGGCGAG GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACAGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCGC TGGAGAAGAC GGGACCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCATGGTGG ATGACAAGAG CGAAGACTCC ATGTCTGTTT CTACCCTGAG CTTTGGCGTT
    AACAGACCCA CAATCTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT GCGCTGCTAC
    CTGTACCAGG CCCGAGACCT GCCAGCCATG GACAAGGACT CCTTCTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCATCA AAGCCAGAAG ACAGTGGTGG AGAAGAACAC CCTGAATCCC
    ACCTGGGATC AAACCCTCAT CTTCTATGAG ATTGAGATCT TCGGCGAGCC AGCCAGTATC
    GCCGAGCATC CGCCCTGCAT CGTGGTGGAG CTCTATGACC ACGACACCTA TGGTGCTGAC
    GAGTTCATGG GTCGCTGTAT TTGTCAGCCA AGCCTGGAAC GGATGCCCCG CCTGGCCTGG
    TTCCCACTGA CCCGGGGCAG CCAGCCTGCC GGCGAGCTGC TGGCAGCGTT CGAGCTTATC
    CAGAGAGAGA AGCCAGCCAT CCATCACATC CCTGGCTTTG AGATGCATGA AACTTCAAGG
    ATTTTGGATG AGACGGAGGA CACAGATTTG CCTTACCCAC CACCTCAAAG GGAGGCCAAC
    ATCTACATGG TTCCCCAGAA CATTAAGCCA GCTCTCCAAC GTACTGCCAT TGAGATCCTG
    GCCTGGGGCC TGCGGAACAT GAAGAGCTAT CAGATGGCCA GCATCTCTTC CCCCAGCCTC
    GTGGTGGAGT GTGGTGGGCA GACAGTGCAG TCCTGTGTCA TCAGAAACCT TCGGAAGAAC
    CCCAACTTTG ATGTCTGTAC TCTCTTCATG GAAGTGATGT TACCCAGAGA GGACCTCTAC
    TGTCCACCTA TTGTGGTCAA GGTCATTGAT AATCGCCAGT TTGGCCGCCG GCCAGTTGTG
    GGTCAGTGTA CCATTCGCTC CCTGGAGAAC TTCCTGTGCG ACCCATACTC AGCAGAGAGT
    CCATCCCCAC AGGGTGGCCC AGATGATGTG AGCTTACTCA GCCCCGGGGA AGATGTGCTG
    ATTGACATCG ATGACAAGGA GCCTCTCATT CCTGTCCAGC TTACAGATGG GCTGTCAAGC
    TTGGGCCCCA CTAACCTGAC GCCTTCTCCA TCCAGTCCTC ATGAGGAGGA GTTCATTGAC
    TGGTGGAGCA AATTCTTTGC TTCTGTCGGG GAGAGGGAAA AGTGTGGTTC CTACCTGGAG
    AAGGATTTTG ACACCTTGAA GGTCTATGAC ACACAGCTGG AGAATGTGGA GGCTTTTGGA
    GGCCTGTCCG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAGGACTCA GGAGGAGACA
    GATGATCCAT CTGTCATCGG AGAATTTAAG GGTCTCTTCA AAATTTATCC CCTCCCAGAA
    GATCCAGCCA TCCCCATGCC CCCGAGACAA TTCCATCAGC TGGCTGCTCA GGGTCCCCAG
    GAATGCTTGG TCCGTATTTA TATCGTCCGA GCATTTGGCT TACAGCCCAA GGACCCCAAT
    GGCAAGTGTG ACCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATCC CTTGCACCCT GGAGCCTGTA TTCGGAAAGA TGTTTGAACT CACCTGCACT
    CTACCTCTGG AGAAGGACCT GAAGATCACA CTCTACGATT ATGACCTGCT CTCCAAGGAT
    GAGAAGATTG GGGAGACGGT CATAGACCTG GAGAACAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGTGGGC TCCCACAGAC CTACTGTGTC TCTGGACCAA ATCAGTGGAG AGATCAGCTC
    CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC CAGCAGCACA GGATCAAGGC CCCCGTGTAC
    CGGACAGACC GAGTGACGTT TCAGGATAAG GACTACACCA TTGAGGAGAT AGAGGCTGGC
    AGACTCCCAA ACCCACACCT GGGCCCAGTG GAGGAACGCT TAGCCCTGCA TGTCCTTCAG
    CAACAAGGCT TGGTTCCTGA GCATGTGGAG TCACGGCCTC TTTATAGTCC TCTGCAGCCA
    GATATCGAGC AGGGGAAGCT ACAGATGTGG ATTGACATAT TTCCAAAGGT GCTGGGCCGG
    CCTGGACCTC CCTTCAACAT CACCCCACGG AAAGCTAGAA GGTTTTTTCT GCGTTGTATT
    ATTTGGAACA CAAAAGATGT GATCTTGGAT GACCTCAGTC TCACGGGGGA GAAGATGAGT
    GACATCTATG TGAAAGGCTG GATGGTGGGA TTCGAAGAAC ACAAGCAGAA GACAGATGTG
    CACTACCGCT CCCTGGGTGG GGAGGGCAAT TTCAACTGGA GGTTCGTGTT TCCCTTCGAC
    TATCTGCCTG CTGAGCAAGT CTGTGCTGTT GCCAAGAAGG ATGCCTTCTG GAGACTCGAC
    AAGACAGAGA GCAAGATCCC AGCACGTGTG GTCTTCCAGA TCTGGGACAA TGACAAATTC
    TCTTTTGATG ACTTTCTGGG CTCTCTGCAG CTGGATCTCA ACCGAATGCC CAAGCCAGCC
    AAGACGGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CCTTCCACCC AGAATGGTTT
    GTGTCCCTTT TTGAGCAGAA GACAGTGAAA GGATGGTGGC CTTGTGTGAC AGAGGAGGGC
    GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA ATGACCTTGG AGATTGTTGC AGAAAGTGAA
    CATGAAGAGC GGCCTGCTGG CCAAGGTCGG GATGAACCCA ACATGAATCC GAAGCTAGAG
    GATCCAAGGC GCCCCGATAC TTCTTTCCTG TGGTTCACCT CCCCGTACAA GACCATGAAG
    TTCATCTTGT GGCGACGCTT CCGGTGTGCC ATCATCCTCT TCATCATCCT CTTCATCCTC
    CTGCTCTTCC TGGGTGTCTT TGTCTACGCC TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506237.1
    CDS255..6569
    Translation

    Target ORF information:

    RefSeq Version XM_006506174.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506174.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTACTCA AGAGTCCACA AGCCACTGAC CTGGACGACA ACCTGGGCTT CCTCCCCACT
    TTTGGGCCCT GCTATGTGAA CCTCTATGGC AGTCCCCGAG AGTTCACGGG CTTCCCAGAT
    CCTTATGCAG AGCTCAACAC AGGAAAGGGA GAAGGTGTGG CTTATCGAGG CAGGGTTCTG
    TTGTCCCTGG AGACCAAACT GGTGGAACAC AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT
    GATGATATCC TGCGTGTGGA GAAGTACCTG CGGAGGCGCA AATATTCCCT CTTTGCTGCC
    TTCTACTCAG CCACAATGCT GCAGGATGTG GATGACGCCA TCCAGTTCGA GGTCAGCATT
    GGGAACTACG GGAACAAGTT CGACACCACC TGCCTGCCCT TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGATGG ATGCCACTAC TATTACTTGC CTTGGGGCAA CGTGAAGCCC
    GTGGTGGTGC TGTCCTCATA CTGGGAAGAC ATCAGCCATC GAATTGAGAT CCAAAACCAG
    CTCCTCAGGG TCGCTGACCG CCTGGAAGCT AACCTGGAGC AGGTCCACTT GGCTCTGAAG
    GCACAGTGTT CCTCCGAGGA TGTGGACGCC CTGGTGGCTC AGTTGACAGA TGAGCTCCTT
    GCAGACTGCA GCCAGCCCCT GTGTGACATC CACGAGATAC CTTCTGCCAC CCACCTTGAT
    CAGTACCTGC TCCGGCTACG CACCCGTCAC CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC
    TTGAAGCTTG GCCATAGTGA GCTCTCCACT GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG
    CATCTCCGTG CCCTGGCCGA GGAGCCCCAG AACAGCCTGC CGGACATCAT CATCTGGATG
    CTGCAGGGTG ACAAGCGTGT GGCCTACCAG CGGGTGCCTG CCCATGAAGT CCTCTTCTCC
    CGGCGGGGCC CCAGCTACTG CGGCAGGAAC TGTGGGAAGC TGCAGACCAT CTTTCTGAAA
    TATCCTATGG AAGGTATGCC TGGGGCCCGA ATGCCCGTGC AGATTCGGAT CAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAATTC AATCAGTTTG CTGAGGGAAA GCTCTCTGTC
    TTTGCCGAAA CGTATGAGAA CCAGACAAAG CTGGCACTGG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACAGGCAAGA TCAAACTACC AAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA GGAGATTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ATGCTGACGC TGGTCATCTG AGCTTCGTGG AGGAGGTGTT CGAGAACCAG
    ACCCGGCTCC CTGGGGGCCA GTGGATTTAC ATGAGTGATA ACTACACAGA TGTGAATGGG
    GAGAAGGTAC TTCCGAAGGA TGATATTGAA TGCCCGCTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCTGTT GATGAACAAG GCTGGGAGTA CAGCATCACC
    ATACCCCCAG ACCGAAAGCC AAAACACTGG GTCCCTGTTG AGAAGATGTA TTACACTCAC
    AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG AGGGACCTCA GCCAGATGGA AGCACTGAAG
    AGGCACAGGC AGGCAGAGGC CGAGGGCGAG GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACAGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCGC TGGAGAAGAC GGGACCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCATGGTGG ATGACAAGAG CGAAGACTCC ATGTCTGTTT CTACCCTGAG CTTTGGCGTT
    AACAGACCCA CAATCTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT GCGCTGCTAC
    CTGTACCAGG CCCGAGACCT GCCAGCCATG GACAAGGACT CCTTCTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCATCA AAGCCAGAAG ACAGTGGTGG AGAAGAACAC CCTGAATCCC
    ACCTGGGATC AAACCCTCAT CTTCTATGAG ATTGAGATCT TCGGCGAGCC AGCCAGTATC
    GCCGAGCATC CGCCCTGCAT CGTGGTGGAG CTCTATGACC ACGACACCTA TGGTGCTGAC
    GAGTTCATGG GTCGCTGTAT TTGTCAGCCA AGCCTGGAAC GGATGCCCCG CCTGGCCTGG
    TTCCCACTGA CCCGGGGCAG CCAGCCTGCC GGCGAGCTGC TGGCAGCGTT CGAGCTTATC
    CAGAGAGAGA AGCCAGCCAT CCATCACATC CCTGGCTTTG AGATGCATGA AACTTCAAGG
    ATTTTGGATG AGACGGAGGA CACAGATTTG CCTTACCCAC CACCTCAAAG GGAGGCCAAC
    ATCTACATGG TTCCCCAGAA CATTAAGCCA GCTCTCCAAC GTACTGCCAT TGAGATCCTG
    GCCTGGGGCC TGCGGAACAT GAAGAGCTAT CAGATGGCCA GCATCTCTTC CCCCAGCCTC
    GTGGTGGAGT GTGGTGGGCA GACAGTGCAG TCCTGTGTCA TCAGAAACCT TCGGAAGAAC
    CCCAACTTTG ATGTCTGTAC TCTCTTCATG GAAGTGATGT TACCCAGAGA GGACCTCTAC
    TGTCCACCTA TTGTGGTCAA GGTCATTGAT AATCGCCAGT TTGGCCGCCG GCCAGTTGTG
    GGTCAGTGTA CCATTCGCTC CCTGGAGAAC TTCCTGTGCG ACCCATACTC AGCAGAGAGT
    CCATCCCCAC AGGGTGGCCC AGATGATGTG AGCTTACTCA GCCCCGGGGA AGATGTGCTG
    ATTGACATCG ATGACAAGGA GCCTCTCATT CCTGTCCAGC TTACAGATGG GCTGTCAAGC
    TTGGGCCCCA CTAACCTGAC GCCTTCTCCA TCCAGTCCTC ATGAGGAGGA GTTCATTGAC
    TGGTGGAGCA AATTCTTTGC TTCTGTCGGG GAGAGGGAAA AGTGTGGTTC CTACCTGGAG
    AAGGATTTTG ACACCTTGAA GGTCTATGAC ACACAGCTGG AGAATGTGGA GGCTTTTGGA
    GGCCTGTCCG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAGGACTCA GGAGGAGACA
    GATGATCCAT CTGTCATCGG AGAATTTAAG GGTCTCTTCA AAATTTATCC CCTCCCAGAA
    GATCCAGCCA TCCCCATGCC CCCGAGACAA TTCCATCAGC TGGCTGCTCA GGGTCCCCAG
    GAATGCTTGG TCCGTATTTA TATCGTCCGA GCATTTGGCT TACAGCCCAA GGACCCCAAT
    GGCAAGTGTG ACCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATCC CTTGCACCCT GGAGCCTGTA TTCGGAAAGA TGTTTGAACT CACCTGCACT
    CTACCTCTGG AGAAGGACCT GAAGATCACA CTCTACGATT ATGACCTGCT CTCCAAGGAT
    GAGAAGATTG GGGAGACGGT CATAGACCTG GAGAACAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGTGGGC TCCCACAGAC CTACTGTGTC TCTGGACCAA ATCAGTGGAG AGATCAGCTC
    CGCCCCTCCC AGCTCCTCCA CCTCTTCTGC CAGCAGCACA GGATCAAGGC CCCCGTGTAC
    CGGACAGACC GAGTGACGTT TCAGGATAAG GACTACACCA TTGAGGAGAT AGAGGCTGGC
    AGACTCCCAA ACCCACACCT GGGCCCAGTG GAGGAACGCT TAGCCCTGCA TGTCCTTCAG
    CAACAAGGCT TGGTTCCTGA GCATGTGGAG TCACGGCCTC TTTATAGTCC TCTGCAGCCA
    GATATCGAGC AGGGGAAGCT ACAGATGTGG ATTGACATAT TTCCAAAGGT GCTGGGCCGG
    CCTGGACCTC CCTTCAACAT CACCCCACGG AAAGCTAGAA GGTTTTTTCT GCGTTGTATT
    ATTTGGAACA CAAAAGATGT GATCTTGGAT GACCTCAGTC TCACGGGGGA GAAGATGAGT
    GACATCTATG TGAAAGGCTG GATGGTGGGA TTCGAAGAAC ACAAGCAGAA GACAGATGTG
    CACTACCGCT CCCTGGGTGG GGAGGGCAAT TTCAACTGGA GGTTCGTGTT TCCCTTCGAC
    TATCTGCCTG CTGAGCAAGT CTGTGCTGTT GCCAAGAAGG ATGCCTTCTG GAGACTCGAC
    AAGACAGAGA GCAAGATCCC AGCACGTGTG GTCTTCCAGA TCTGGGACAA TGACAAATTC
    TCTTTTGATG ACTTTCTGGG CTCTCTGCAG CTGGATCTCA ACCGAATGCC CAAGCCAGCC
    AAGACGGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CCTTCCACCC AGAATGGTTT
    GTGTCCCTTT TTGAGCAGAA GACAGTGAAA GGATGGTGGC CTTGTGTGAC AGAGGAGGGC
    GAGAAGAAGA TGTTGGCGGG CAAGCTGGAA ATGACCTTGG AGATTGTTGC AGAAAGTGAA
    CATGAAGAGC GGCCTGCTGG CCAAGGTCGG GATGAACCCA ACATGAATCC GAAGCTAGAG
    GATCCAAGGC GCCCCGATAC TTCTTTCCTG TGGTTCACCT CCCCGTACAA GACCATGAAG
    TTCATCTTGT GGCGACGCTT CCGGTGTGCC ATCATCCTCT TCATCATCCT CTTCATCCTC
    CTGCTCTTCC TGGGTGTCTT TGTCTACGCC TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTTC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37303
    Clone ID Related Accession (Same CDS sequence) XM_006506175.3 , XM_006506175.4
    Accession Version XM_006506175.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTACTCAAGA GTCCACAAGC CACTGACCTG GACGACAACC TGGGCTTCCT CCCCACTTTT
    GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT
    TATGCAGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCTT ATCGAGGCAG GGTTCTGTTG
    TCCCTGGAGA CCAAACTGGT GGAACACAGT GAGCAGAAGG TGGAAGACCT TCCTGCTGAT
    GATATCCTGC GTGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC
    TACTCAGCCA CAATGCTGCA GGATGTGGAT GACGCCATCC AGTTCGAGGT CAGCATTGGG
    AACTACGGGA ACAAGTTCGA CACCACCTGC CTGCCCTTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGATGGATG CCACTACTAT TACTTGCCTT GGGGCAACGT GAAGCCCGTG
    GTGGTGCTGT CCTCATACTG GGAAGACATC AGCCATCGAA TTGAGATCCA AAACCAGCTC
    CTCAGGGTCG CTGACCGCCT GGAAGCTAAC CTGGAGCAGG TCCACTTGGC TCTGAAGGCA
    CAGTGTTCCT CCGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA
    GACTGCAGCC AGCCCCTGTG TGACATCCAC GAGATACCTT CTGCCACCCA CCTTGATCAG
    TACCTGCTCC GGCTACGCAC CCGTCACCTG AGCCAGATCA AAGAAGCTGC TCTGGCCTTG
    AAGCTTGGCC ATAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT
    CTCCGTGCCC TGGCCGAGGA GCCCCAGAAC AGCCTGCCGG ACATCATCAT CTGGATGCTG
    CAGGGTGACA AGCGTGTGGC CTACCAGCGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG
    CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAAGCTGC AGACCATCTT TCTGAAATAT
    CCTATGGAAG GTATGCCTGG GGCCCGAATG CCCGTGCAGA TTCGGATCAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAATTCAAT CAGTTTGCTG AGGGAAAGCT CTCTGTCTTT
    GCCGAAACGT ATGAGAACCA GACAAAGCTG GCACTGGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGATGTCACA GGCAAGATCA AACTACCAAA GGATAGCTTC
    CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GATTGGTTCG TGTGTCCAGA GAAGACCCTA
    CTCCATGATG CTGACGCTGG TCATCTGAGC TTCGTGGAGG AGGTGTTCGA GAACCAGACC
    CGGCTCCCTG GGGGCCAGTG GATTTACATG AGTGATAACT ACACAGATGT GAATGGGGAG
    AAGGTACTTC CGAAGGATGA TATTGAATGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA
    CCCCCAGACC GAAAGCCAAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG
    CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAAGC ACTGAAGAGG
    CACAGGCAGG CAGAGGCCGA GGGCGAGGGC TGGGAGTATG CCTCTCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACAGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCGCTGG AGAAGACGGG ACCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    ATGGTGGATG ACAAGAGCGA AGACTCCATG TCTGTTTCTA CCCTGAGCTT TGGCGTTAAC
    AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG CTGCTACCTG
    TACCAGGCCC GAGACCTGCC AGCCATGGAC AAGGACTCCT TCTCTGATCC CTACGCCATC
    GTCTCCTTCC TGCATCAAAG CCAGAAGACA GTGGTGGAGA AGAACACCCT GAATCCCACC
    TGGGATCAAA CCCTCATCTT CTATGAGATT GAGATCTTCG GCGAGCCAGC CAGTATCGCC
    GAGCATCCGC CCTGCATCGT GGTGGAGCTC TATGACCACG ACACCTATGG TGCTGACGAG
    TTCATGGGTC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC
    CCACTGACCC GGGGCAGCCA GCCTGCCGGC GAGCTGCTGG CAGCGTTCGA GCTTATCCAG
    AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCATGAAAC TTCAAGGATT
    TTGGATGAGA CGGAGGACAC AGATTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC
    TACATGGTTC CCCAGAACAT TAAGCCAGCT CTCCAACGTA CTGCCATTGA GATCCTGGCC
    TGGGGCCTGC GGAACATGAA GAGCTATCAG ATGGCCAGCA TCTCTTCCCC CAGCCTCGTG
    GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC
    AACTTTGATG TCTGTACTCT CTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT
    CCACCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCCGGCC AGTTGTGGGT
    CAGTGTACCA TTCGCTCCCT GGAGAACTTC CTGTGCGACC CATACTCAGC AGAGAGTCCA
    TCCCCACAGG GTGGCCCAGA TGATGTGAGC TTACTCAGCC CCGGGGAAGA TGTGCTGATT
    GACATCGATG ACAAGGAGCC TCTCATTCCT GTCCAGCTTA CAGATGGGCT GTCAAGCTTG
    GGCCCCACTA ACCTGACGCC TTCTCCATCC AGTCCTCATG AGGAGGAGTT CATTGACTGG
    TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG
    GATTTTGACA CCTTGAAGGT CTATGACACA CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC
    CTGTCCGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA GGACTCAGGA GGAGACAGAT
    GATCCATCTG TCATCGGAGA ATTTAAGGGT CTCTTCAAAA TTTATCCCCT CCCAGAAGAT
    CCAGCCATCC CCATGCCCCC GAGACAATTC CATCAGCTGG CTGCTCAGGG TCCCCAGGAA
    TGCTTGGTCC GTATTTATAT CGTCCGAGCA TTTGGCTTAC AGCCCAAGGA CCCCAATGGC
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATCCCTT GCACCCTGGA GCCTGTATTC GGAAAGATGT TTGAACTCAC CTGCACTCTA
    CCTCTGGAGA AGGACCTGAA GATCACACTC TACGATTATG ACCTGCTCTC CAAGGATGAG
    AAGATTGGGG AGACGGTCAT AGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGTGGGCTCC CACAGACCTA CTGTGTCTCT GGACCAAATC AGTGGAGAGA TCAGCTCCGC
    CCCTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCACAGGA TCAAGGCCCC CGTGTACCGG
    ACAGACCGAG TGACGTTTCA GGATAAGGAC TACACCATTG AGGAGATAGA GGCTGGCAGA
    CTCCCAAACC CACACCTGGG CCCAGTGGAG GAACGCTTAG CCCTGCATGT CCTTCAGCAA
    CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT
    ATCGAGCAGG GGAAGCTACA GATGTGGATT GACATATTTC CAAAGGTGCT GGGCCGGCCT
    GGACCTCCCT TCAACATCAC CCCACGGAAA GCTAGAAGGT TTTTTCTGCG TTGTATTATT
    TGGAACACAA AAGATGTGAT CTTGGATGAC CTCAGTCTCA CGGGGGAGAA GATGAGTGAC
    ATCTATGTGA AAGGCTGGAT GGTGGGATTC GAAGAACACA AGCAGAAGAC AGATGTGCAC
    TACCGCTCCC TGGGTGGGGA GGGCAATTTC AACTGGAGGT TCGTGTTTCC CTTCGACTAT
    CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC AAGAAGGATG CCTTCTGGAG ACTCGACAAG
    ACAGAGAGCA AGATCCCAGC ACGTGTGGTC TTCCAGATCT GGGACAATGA CAAATTCTCT
    TTTGATGACT TTCTGGGCTC TCTGCAGCTG GATCTCAACC GAATGCCCAA GCCAGCCAAG
    ACGGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACCT TCCACCCAGA ATGGTTTGTG
    TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA TGGTGGCCTT GTGTGACAGA GGAGGGCGAG
    AAGAAGATGT TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TTGTTGCAGA AAGTGAACAT
    GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT GAACCCAACA TGAATCCGAA GCTAGAGGAT
    CCAAGGCGCC CCGATACTTC TTTCCTGTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC
    ATCTTGTGGC GACGCTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTCCTG
    CTCTTCCTGG GTGTCTTTGT CTACGCCTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506238.1
    CDS601..6912
    Translation

    Target ORF information:

    RefSeq Version XM_006506175.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506175.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTACTCAAGA GTCCACAAGC CACTGACCTG GACGACAACC TGGGCTTCCT CCCCACTTTT
    GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT
    TATGCAGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCTT ATCGAGGCAG GGTTCTGTTG
    TCCCTGGAGA CCAAACTGGT GGAACACAGT GAGCAGAAGG TGGAAGACCT TCCTGCTGAT
    GATATCCTGC GTGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC
    TACTCAGCCA CAATGCTGCA GGATGTGGAT GACGCCATCC AGTTCGAGGT CAGCATTGGG
    AACTACGGGA ACAAGTTCGA CACCACCTGC CTGCCCTTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGATGGATG CCACTACTAT TACTTGCCTT GGGGCAACGT GAAGCCCGTG
    GTGGTGCTGT CCTCATACTG GGAAGACATC AGCCATCGAA TTGAGATCCA AAACCAGCTC
    CTCAGGGTCG CTGACCGCCT GGAAGCTAAC CTGGAGCAGG TCCACTTGGC TCTGAAGGCA
    CAGTGTTCCT CCGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA
    GACTGCAGCC AGCCCCTGTG TGACATCCAC GAGATACCTT CTGCCACCCA CCTTGATCAG
    TACCTGCTCC GGCTACGCAC CCGTCACCTG AGCCAGATCA AAGAAGCTGC TCTGGCCTTG
    AAGCTTGGCC ATAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT
    CTCCGTGCCC TGGCCGAGGA GCCCCAGAAC AGCCTGCCGG ACATCATCAT CTGGATGCTG
    CAGGGTGACA AGCGTGTGGC CTACCAGCGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG
    CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAAGCTGC AGACCATCTT TCTGAAATAT
    CCTATGGAAG GTATGCCTGG GGCCCGAATG CCCGTGCAGA TTCGGATCAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAATTCAAT CAGTTTGCTG AGGGAAAGCT CTCTGTCTTT
    GCCGAAACGT ATGAGAACCA GACAAAGCTG GCACTGGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGATGTCACA GGCAAGATCA AACTACCAAA GGATAGCTTC
    CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GATTGGTTCG TGTGTCCAGA GAAGACCCTA
    CTCCATGATG CTGACGCTGG TCATCTGAGC TTCGTGGAGG AGGTGTTCGA GAACCAGACC
    CGGCTCCCTG GGGGCCAGTG GATTTACATG AGTGATAACT ACACAGATGT GAATGGGGAG
    AAGGTACTTC CGAAGGATGA TATTGAATGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA
    CCCCCAGACC GAAAGCCAAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG
    CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAAGC ACTGAAGAGG
    CACAGGCAGG CAGAGGCCGA GGGCGAGGGC TGGGAGTATG CCTCTCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACAGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCGCTGG AGAAGACGGG ACCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    ATGGTGGATG ACAAGAGCGA AGACTCCATG TCTGTTTCTA CCCTGAGCTT TGGCGTTAAC
    AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG CTGCTACCTG
    TACCAGGCCC GAGACCTGCC AGCCATGGAC AAGGACTCCT TCTCTGATCC CTACGCCATC
    GTCTCCTTCC TGCATCAAAG CCAGAAGACA GTGGTGGAGA AGAACACCCT GAATCCCACC
    TGGGATCAAA CCCTCATCTT CTATGAGATT GAGATCTTCG GCGAGCCAGC CAGTATCGCC
    GAGCATCCGC CCTGCATCGT GGTGGAGCTC TATGACCACG ACACCTATGG TGCTGACGAG
    TTCATGGGTC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC
    CCACTGACCC GGGGCAGCCA GCCTGCCGGC GAGCTGCTGG CAGCGTTCGA GCTTATCCAG
    AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCATGAAAC TTCAAGGATT
    TTGGATGAGA CGGAGGACAC AGATTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC
    TACATGGTTC CCCAGAACAT TAAGCCAGCT CTCCAACGTA CTGCCATTGA GATCCTGGCC
    TGGGGCCTGC GGAACATGAA GAGCTATCAG ATGGCCAGCA TCTCTTCCCC CAGCCTCGTG
    GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC
    AACTTTGATG TCTGTACTCT CTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT
    CCACCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCCGGCC AGTTGTGGGT
    CAGTGTACCA TTCGCTCCCT GGAGAACTTC CTGTGCGACC CATACTCAGC AGAGAGTCCA
    TCCCCACAGG GTGGCCCAGA TGATGTGAGC TTACTCAGCC CCGGGGAAGA TGTGCTGATT
    GACATCGATG ACAAGGAGCC TCTCATTCCT GTCCAGCTTA CAGATGGGCT GTCAAGCTTG
    GGCCCCACTA ACCTGACGCC TTCTCCATCC AGTCCTCATG AGGAGGAGTT CATTGACTGG
    TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG
    GATTTTGACA CCTTGAAGGT CTATGACACA CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC
    CTGTCCGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA GGACTCAGGA GGAGACAGAT
    GATCCATCTG TCATCGGAGA ATTTAAGGGT CTCTTCAAAA TTTATCCCCT CCCAGAAGAT
    CCAGCCATCC CCATGCCCCC GAGACAATTC CATCAGCTGG CTGCTCAGGG TCCCCAGGAA
    TGCTTGGTCC GTATTTATAT CGTCCGAGCA TTTGGCTTAC AGCCCAAGGA CCCCAATGGC
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATCCCTT GCACCCTGGA GCCTGTATTC GGAAAGATGT TTGAACTCAC CTGCACTCTA
    CCTCTGGAGA AGGACCTGAA GATCACACTC TACGATTATG ACCTGCTCTC CAAGGATGAG
    AAGATTGGGG AGACGGTCAT AGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGTGGGCTCC CACAGACCTA CTGTGTCTCT GGACCAAATC AGTGGAGAGA TCAGCTCCGC
    CCCTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCACAGGA TCAAGGCCCC CGTGTACCGG
    ACAGACCGAG TGACGTTTCA GGATAAGGAC TACACCATTG AGGAGATAGA GGCTGGCAGA
    CTCCCAAACC CACACCTGGG CCCAGTGGAG GAACGCTTAG CCCTGCATGT CCTTCAGCAA
    CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT
    ATCGAGCAGG GGAAGCTACA GATGTGGATT GACATATTTC CAAAGGTGCT GGGCCGGCCT
    GGACCTCCCT TCAACATCAC CCCACGGAAA GCTAGAAGGT TTTTTCTGCG TTGTATTATT
    TGGAACACAA AAGATGTGAT CTTGGATGAC CTCAGTCTCA CGGGGGAGAA GATGAGTGAC
    ATCTATGTGA AAGGCTGGAT GGTGGGATTC GAAGAACACA AGCAGAAGAC AGATGTGCAC
    TACCGCTCCC TGGGTGGGGA GGGCAATTTC AACTGGAGGT TCGTGTTTCC CTTCGACTAT
    CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC AAGAAGGATG CCTTCTGGAG ACTCGACAAG
    ACAGAGAGCA AGATCCCAGC ACGTGTGGTC TTCCAGATCT GGGACAATGA CAAATTCTCT
    TTTGATGACT TTCTGGGCTC TCTGCAGCTG GATCTCAACC GAATGCCCAA GCCAGCCAAG
    ACGGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACCT TCCACCCAGA ATGGTTTGTG
    TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA TGGTGGCCTT GTGTGACAGA GGAGGGCGAG
    AAGAAGATGT TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TTGTTGCAGA AAGTGAACAT
    GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT GAACCCAACA TGAATCCGAA GCTAGAGGAT
    CCAAGGCGCC CCGATACTTC TTTCCTGTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC
    ATCTTGTGGC GACGCTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTCCTG
    CTCTTCCTGG GTGTCTTTGT CTACGCCTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37304
    Clone ID Related Accession (Same CDS sequence) XM_006506176.2
    Accession Version XM_006506176.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGT GGACGACAAC
    CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG
    TTCACGGGCT TCCCAGATCC TTATGCAGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCT
    TATCGAGGCA GGGTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAACACAG TGAGCAGAAG
    GTGGAAGACC TTCCTGCTGA TGATATCCTG CGTGTGGAGA AGTACCTGCG GAGGCGCAAA
    TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGACGCCATC
    CAGTTCGAGG TCAGCATTGG GAACTACGGG AACAAGTTCG ACACCACCTG CCTGCCCTTG
    GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGATGGAT GCCACTACTA TTACTTGCCT
    TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCCTCATACT GGGAAGACAT CAGCCATCGA
    ATTGAGATCC AAAACCAGCT CCTCAGGGTC GCTGACCGCC TGGAAGCTAA CCTGGAGCAG
    GTCCACTTGG CTCTGAAGGC ACAGTGTTCC TCCGAGGATG TGGACGCCCT GGTGGCTCAG
    TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA CGAGATACCT
    TCTGCCACCC ACCTTGATCA GTACCTGCTC CGGCTACGCA CCCGTCACCT GAGCCAGATC
    AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC CATAGTGAGC TCTCCACTGC CCTGGAGCAG
    GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG
    GACATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAGCG GGTGCCTGCC
    CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAAGCTG
    CAGACCATCT TTCTGAAATA TCCTATGGAA GGTATGCCTG GGGCCCGAAT GCCCGTGCAG
    ATTCGGATCA AGCTCTGGTT CGGGCTCTCT GTGGATGAGA AGGAATTCAA TCAGTTTGCT
    GAGGGAAAGC TCTCTGTCTT TGCCGAAACG TATGAGAACC AGACAAAGCT GGCACTGGTC
    GGGAACTGGG GCACAACGGG CCTCACCTAC CCCAAGTTTT CTGATGTCAC AGGCAAGATC
    AAACTACCAA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGATTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAT GCTGACGCTG GTCATCTGAG CTTCGTGGAG
    GAGGTGTTCG AGAACCAGAC CCGGCTCCCT GGGGGCCAGT GGATTTACAT GAGTGATAAC
    TACACAGATG TGAATGGGGA GAAGGTACTT CCGAAGGATG ATATTGAATG CCCGCTGGGC
    TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC
    TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCAA AACACTGGGT CCCTGTTGAG
    AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC
    CAGATGGAAG CACTGAAGAG GCACAGGCAG GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT
    GCCTCTCTCT TCGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG GAGAAGACGG GACCTGCAGC TGTGTTTGCC
    CTCGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGACTCCAT GTCTGTTTCT
    ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC
    TACCATCTGC GCTGCTACCT GTACCAGGCC CGAGACCTGC CAGCCATGGA CAAGGACTCC
    TTCTCTGATC CCTACGCCAT CGTCTCCTTC CTGCATCAAA GCCAGAAGAC AGTGGTGGAG
    AAGAACACCC TGAATCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT TGAGATCTTC
    GGCGAGCCAG CCAGTATCGC CGAGCATCCG CCCTGCATCG TGGTGGAGCT CTATGACCAC
    GACACCTATG GTGCTGACGA GTTCATGGGT CGCTGTATTT GTCAGCCAAG CCTGGAACGG
    ATGCCCCGCC TGGCCTGGTT CCCACTGACC CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG
    GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG
    ATGCATGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGATTTGCC TTACCCACCA
    CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TTAAGCCAGC TCTCCAACGT
    ACTGCCATTG AGATCCTGGC CTGGGGCCTG CGGAACATGA AGAGCTATCA GATGGCCAGC
    ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC
    AGAAACCTTC GGAAGAACCC CAACTTTGAT GTCTGTACTC TCTTCATGGA AGTGATGTTA
    CCCAGAGAGG ACCTCTACTG TCCACCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT
    GGCCGCCGGC CAGTTGTGGG TCAGTGTACC ATTCGCTCCC TGGAGAACTT CCTGTGCGAC
    CCATACTCAG CAGAGAGTCC ATCCCCACAG GGTGGCCCAG ATGATGTGAG CTTACTCAGC
    CCCGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CTCTCATTCC TGTCCAGCTT
    ACAGATGGGC TGTCAAGCTT GGGCCCCACT AACCTGACGC CTTCTCCATC CAGTCCTCAT
    GAGGAGGAGT TCATTGACTG GTGGAGCAAA TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG
    TGTGGTTCCT ACCTGGAGAA GGATTTTGAC ACCTTGAAGG TCTATGACAC ACAGCTGGAG
    AATGTGGAGG CTTTTGGAGG CCTGTCCGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AGGACTCAGG AGGAGACAGA TGATCCATCT GTCATCGGAG AATTTAAGGG TCTCTTCAAA
    ATTTATCCCC TCCCAGAAGA TCCAGCCATC CCCATGCCCC CGAGACAATT CCATCAGCTG
    GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC CGTATTTATA TCGTCCGAGC ATTTGGCTTA
    CAGCCCAAGG ACCCCAATGG CAAGTGTGAC CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATCCCT TGCACCCTGG AGCCTGTATT CGGAAAGATG
    TTTGAACTCA CCTGCACTCT ACCTCTGGAG AAGGACCTGA AGATCACACT CTACGATTAT
    GACCTGCTCT CCAAGGATGA GAAGATTGGG GAGACGGTCA TAGACCTGGA GAACAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC CCACAGACCT ACTGTGTCTC TGGACCAAAT
    CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCACAGG
    ATCAAGGCCC CCGTGTACCG GACAGACCGA GTGACGTTTC AGGATAAGGA CTACACCATT
    GAGGAGATAG AGGCTGGCAG ACTCCCAAAC CCACACCTGG GCCCAGTGGA GGAACGCTTA
    GCCCTGCATG TCCTTCAGCA ACAAGGCTTG GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT
    TATAGTCCTC TGCAGCCAGA TATCGAGCAG GGGAAGCTAC AGATGTGGAT TGACATATTT
    CCAAAGGTGC TGGGCCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAA AGCTAGAAGG
    TTTTTTCTGC GTTGTATTAT TTGGAACACA AAAGATGTGA TCTTGGATGA CCTCAGTCTC
    ACGGGGGAGA AGATGAGTGA CATCTATGTG AAAGGCTGGA TGGTGGGATT CGAAGAACAC
    AAGCAGAAGA CAGATGTGCA CTACCGCTCC CTGGGTGGGG AGGGCAATTT CAACTGGAGG
    TTCGTGTTTC CCTTCGACTA TCTGCCTGCT GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT
    GCCTTCTGGA GACTCGACAA GACAGAGAGC AAGATCCCAG CACGTGTGGT CTTCCAGATC
    TGGGACAATG ACAAATTCTC TTTTGATGAC TTTCTGGGCT CTCTGCAGCT GGATCTCAAC
    CGAATGCCCA AGCCAGCCAA GACGGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACC
    TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT
    TGTGTGACAG AGGAGGGCGA GAAGAAGATG TTGGCGGGCA AGCTGGAAAT GACCTTGGAG
    ATTGTTGCAG AAAGTGAACA TGAAGAGCGG CCTGCTGGCC AAGGTCGGGA TGAACCCAAC
    ATGAATCCGA AGCTAGAGGA TCCAAGGCGC CCCGATACTT CTTTCCTGTG GTTCACCTCC
    CCGTACAAGA CCATGAAGTT CATCTTGTGG CGACGCTTCC GGTGTGCCAT CATCCTCTTC
    ATCATCCTCT TCATCCTCCT GCTCTTCCTG GGTGTCTTTG TCTACGCCTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTTCGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506239.1
    CDS255..6527
    Translation

    Target ORF information:

    RefSeq Version XM_006506176.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506176.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGT GGACGACAAC
    CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG
    TTCACGGGCT TCCCAGATCC TTATGCAGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCT
    TATCGAGGCA GGGTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAACACAG TGAGCAGAAG
    GTGGAAGACC TTCCTGCTGA TGATATCCTG CGTGTGGAGA AGTACCTGCG GAGGCGCAAA
    TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGACGCCATC
    CAGTTCGAGG TCAGCATTGG GAACTACGGG AACAAGTTCG ACACCACCTG CCTGCCCTTG
    GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGATGGAT GCCACTACTA TTACTTGCCT
    TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCCTCATACT GGGAAGACAT CAGCCATCGA
    ATTGAGATCC AAAACCAGCT CCTCAGGGTC GCTGACCGCC TGGAAGCTAA CCTGGAGCAG
    GTCCACTTGG CTCTGAAGGC ACAGTGTTCC TCCGAGGATG TGGACGCCCT GGTGGCTCAG
    TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA CGAGATACCT
    TCTGCCACCC ACCTTGATCA GTACCTGCTC CGGCTACGCA CCCGTCACCT GAGCCAGATC
    AAAGAAGCTG CTCTGGCCTT GAAGCTTGGC CATAGTGAGC TCTCCACTGC CCTGGAGCAG
    GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCCGAGG AGCCCCAGAA CAGCCTGCCG
    GACATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAGCG GGTGCCTGCC
    CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAAGCTG
    CAGACCATCT TTCTGAAATA TCCTATGGAA GGTATGCCTG GGGCCCGAAT GCCCGTGCAG
    ATTCGGATCA AGCTCTGGTT CGGGCTCTCT GTGGATGAGA AGGAATTCAA TCAGTTTGCT
    GAGGGAAAGC TCTCTGTCTT TGCCGAAACG TATGAGAACC AGACAAAGCT GGCACTGGTC
    GGGAACTGGG GCACAACGGG CCTCACCTAC CCCAAGTTTT CTGATGTCAC AGGCAAGATC
    AAACTACCAA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGATTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAT GCTGACGCTG GTCATCTGAG CTTCGTGGAG
    GAGGTGTTCG AGAACCAGAC CCGGCTCCCT GGGGGCCAGT GGATTTACAT GAGTGATAAC
    TACACAGATG TGAATGGGGA GAAGGTACTT CCGAAGGATG ATATTGAATG CCCGCTGGGC
    TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC
    TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCAA AACACTGGGT CCCTGTTGAG
    AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC
    CAGATGGAAG CACTGAAGAG GCACAGGCAG GCAGAGGCCG AGGGCGAGGG CTGGGAGTAT
    GCCTCTCTCT TCGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAGCCGCTG GAGAAGACGG GACCTGCAGC TGTGTTTGCC
    CTCGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGACTCCAT GTCTGTTTCT
    ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC
    TACCATCTGC GCTGCTACCT GTACCAGGCC CGAGACCTGC CAGCCATGGA CAAGGACTCC
    TTCTCTGATC CCTACGCCAT CGTCTCCTTC CTGCATCAAA GCCAGAAGAC AGTGGTGGAG
    AAGAACACCC TGAATCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT TGAGATCTTC
    GGCGAGCCAG CCAGTATCGC CGAGCATCCG CCCTGCATCG TGGTGGAGCT CTATGACCAC
    GACACCTATG GTGCTGACGA GTTCATGGGT CGCTGTATTT GTCAGCCAAG CCTGGAACGG
    ATGCCCCGCC TGGCCTGGTT CCCACTGACC CGGGGCAGCC AGCCTGCCGG CGAGCTGCTG
    GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG
    ATGCATGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGATTTGCC TTACCCACCA
    CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TTAAGCCAGC TCTCCAACGT
    ACTGCCATTG AGATCCTGGC CTGGGGCCTG CGGAACATGA AGAGCTATCA GATGGCCAGC
    ATCTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC
    AGAAACCTTC GGAAGAACCC CAACTTTGAT GTCTGTACTC TCTTCATGGA AGTGATGTTA
    CCCAGAGAGG ACCTCTACTG TCCACCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT
    GGCCGCCGGC CAGTTGTGGG TCAGTGTACC ATTCGCTCCC TGGAGAACTT CCTGTGCGAC
    CCATACTCAG CAGAGAGTCC ATCCCCACAG GGTGGCCCAG ATGATGTGAG CTTACTCAGC
    CCCGGGGAAG ATGTGCTGAT TGACATCGAT GACAAGGAGC CTCTCATTCC TGTCCAGCTT
    ACAGATGGGC TGTCAAGCTT GGGCCCCACT AACCTGACGC CTTCTCCATC CAGTCCTCAT
    GAGGAGGAGT TCATTGACTG GTGGAGCAAA TTCTTTGCTT CTGTCGGGGA GAGGGAAAAG
    TGTGGTTCCT ACCTGGAGAA GGATTTTGAC ACCTTGAAGG TCTATGACAC ACAGCTGGAG
    AATGTGGAGG CTTTTGGAGG CCTGTCCGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AGGACTCAGG AGGAGACAGA TGATCCATCT GTCATCGGAG AATTTAAGGG TCTCTTCAAA
    ATTTATCCCC TCCCAGAAGA TCCAGCCATC CCCATGCCCC CGAGACAATT CCATCAGCTG
    GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC CGTATTTATA TCGTCCGAGC ATTTGGCTTA
    CAGCCCAAGG ACCCCAATGG CAAGTGTGAC CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATCCCT TGCACCCTGG AGCCTGTATT CGGAAAGATG
    TTTGAACTCA CCTGCACTCT ACCTCTGGAG AAGGACCTGA AGATCACACT CTACGATTAT
    GACCTGCTCT CCAAGGATGA GAAGATTGGG GAGACGGTCA TAGACCTGGA GAACAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGTGGGCTC CCACAGACCT ACTGTGTCTC TGGACCAAAT
    CAGTGGAGAG ATCAGCTCCG CCCCTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCACAGG
    ATCAAGGCCC CCGTGTACCG GACAGACCGA GTGACGTTTC AGGATAAGGA CTACACCATT
    GAGGAGATAG AGGCTGGCAG ACTCCCAAAC CCACACCTGG GCCCAGTGGA GGAACGCTTA
    GCCCTGCATG TCCTTCAGCA ACAAGGCTTG GTTCCTGAGC ATGTGGAGTC ACGGCCTCTT
    TATAGTCCTC TGCAGCCAGA TATCGAGCAG GGGAAGCTAC AGATGTGGAT TGACATATTT
    CCAAAGGTGC TGGGCCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAA AGCTAGAAGG
    TTTTTTCTGC GTTGTATTAT TTGGAACACA AAAGATGTGA TCTTGGATGA CCTCAGTCTC
    ACGGGGGAGA AGATGAGTGA CATCTATGTG AAAGGCTGGA TGGTGGGATT CGAAGAACAC
    AAGCAGAAGA CAGATGTGCA CTACCGCTCC CTGGGTGGGG AGGGCAATTT CAACTGGAGG
    TTCGTGTTTC CCTTCGACTA TCTGCCTGCT GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT
    GCCTTCTGGA GACTCGACAA GACAGAGAGC AAGATCCCAG CACGTGTGGT CTTCCAGATC
    TGGGACAATG ACAAATTCTC TTTTGATGAC TTTCTGGGCT CTCTGCAGCT GGATCTCAAC
    CGAATGCCCA AGCCAGCCAA GACGGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACC
    TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT
    TGTGTGACAG AGGAGGGCGA GAAGAAGATG TTGGCGGGCA AGCTGGAAAT GACCTTGGAG
    ATTGTTGCAG AAAGTGAACA TGAAGAGCGG CCTGCTGGCC AAGGTCGGGA TGAACCCAAC
    ATGAATCCGA AGCTAGAGGA TCCAAGGCGC CCCGATACTT CTTTCCTGTG GTTCACCTCC
    CCGTACAAGA CCATGAAGTT CATCTTGTGG CGACGCTTCC GGTGTGCCAT CATCCTCTTC
    ATCATCCTCT TCATCCTCCT GCTCTTCCTG GGTGTCTTTG TCTACGCCTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTTCGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37305
    Clone ID Related Accession (Same CDS sequence) XM_006506177.4 , XM_006506177.3
    Accession Version XM_006506177.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGTGGA CGACAACCTG
    GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC
    ACGGGCTTCC CAGATCCTTA TGCAGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCTTAT
    CGAGGCAGGG TTCTGTTGTC CCTGGAGACC AAACTGGTGG AACACAGTGA GCAGAAGGTG
    GAAGACCTTC CTGCTGATGA TATCCTGCGT GTGGAGAAGT ACCTGCGGAG GCGCAAATAT
    TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA CGCCATCCAG
    TTCGAGGTCA GCATTGGGAA CTACGGGAAC AAGTTCGACA CCACCTGCCT GCCCTTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTATTA CTTGCCTTGG
    GGCAACGTGA AGCCCGTGGT GGTGCTGTCC TCATACTGGG AAGACATCAG CCATCGAATT
    GAGATCCAAA ACCAGCTCCT CAGGGTCGCT GACCGCCTGG AAGCTAACCT GGAGCAGGTC
    CACTTGGCTC TGAAGGCACA GTGTTCCTCC GAGGATGTGG ACGCCCTGGT GGCTCAGTTG
    ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCACGA GATACCTTCT
    GCCACCCACC TTGATCAGTA CCTGCTCCGG CTACGCACCC GTCACCTGAG CCAGATCAAA
    GAAGCTGCTC TGGCCTTGAA GCTTGGCCAT AGTGAGCTCT CCACTGCCCT GGAGCAGGCC
    GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCCGAGGAGC CCCAGAACAG CCTGCCGGAC
    ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAGCGGGT GCCTGCCCAT
    GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAAGCTGCAG
    ACCATCTTTC TGAAATATCC TATGGAAGGT ATGCCTGGGG CCCGAATGCC CGTGCAGATT
    CGGATCAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AATTCAATCA GTTTGCTGAG
    GGAAAGCTCT CTGTCTTTGC CGAAACGTAT GAGAACCAGA CAAAGCTGGC ACTGGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ATGTCACAGG CAAGATCAAA
    CTACCAAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA TTGGTTCGTG
    TGTCCAGAGA AGACCCTACT CCATGATGCT GACGCTGGTC ATCTGAGCTT CGTGGAGGAG
    GTGTTCGAGA ACCAGACCCG GCTCCCTGGG GGCCAGTGGA TTTACATGAG TGATAACTAC
    ACAGATGTGA ATGGGGAGAA GGTACTTCCG AAGGATGATA TTGAATGCCC GCTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG
    GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCAAAAC ACTGGGTCCC TGTTGAGAAG
    ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAAGCAC TGAAGAGGCA CAGGCAGGCA GAGGCCGAGG GCGAGGGCTG GGAGTATGCC
    TCTCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGAC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ACTCCATGTC TGTTTCTACC
    CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC
    CATCTGCGCT GCTACCTGTA CCAGGCCCGA GACCTGCCAG CCATGGACAA GGACTCCTTC
    TCTGATCCCT ACGCCATCGT CTCCTTCCTG CATCAAAGCC AGAAGACAGT GGTGGAGAAG
    AACACCCTGA ATCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATTGA GATCTTCGGC
    GAGCCAGCCA GTATCGCCGA GCATCCGCCC TGCATCGTGG TGGAGCTCTA TGACCACGAC
    ACCTATGGTG CTGACGAGTT CATGGGTCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG
    CCCCGCCTGG CCTGGTTCCC ACTGACCCGG GGCAGCCAGC CTGCCGGCGA GCTGCTGGCA
    GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG
    CATGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ATTTGCCTTA CCCACCACCT
    CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATTA AGCCAGCTCT CCAACGTACT
    GCCATTGAGA TCCTGGCCTG GGGCCTGCGG AACATGAAGA GCTATCAGAT GGCCAGCATC
    TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA
    AACCTTCGGA AGAACCCCAA CTTTGATGTC TGTACTCTCT TCATGGAAGT GATGTTACCC
    AGAGAGGACC TCTACTGTCC ACCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC
    CGCCGGCCAG TTGTGGGTCA GTGTACCATT CGCTCCCTGG AGAACTTCCT GTGCGACCCA
    TACTCAGCAG AGAGTCCATC CCCACAGGGT GGCCCAGATG ATGTGAGCTT ACTCAGCCCC
    GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCTC TCATTCCTGT CCAGCTTACA
    GATGGGCTGT CAAGCTTGGG CCCCACTAAC CTGACGCCTT CTCCATCCAG TCCTCATGAG
    GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT
    GGTTCCTACC TGGAGAAGGA TTTTGACACC TTGAAGGTCT ATGACACACA GCTGGAGAAT
    GTGGAGGCTT TTGGAGGCCT GTCCGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAGG
    ACTCAGGAGG AGACAGATGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT
    TATCCCCTCC CAGAAGATCC AGCCATCCCC ATGCCCCCGA GACAATTCCA TCAGCTGGCT
    GCTCAGGGTC CCCAGGAATG CTTGGTCCGT ATTTATATCG TCCGAGCATT TGGCTTACAG
    CCCAAGGACC CCAATGGCAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATCCCTTGC ACCCTGGAGC CTGTATTCGG AAAGATGTTT
    GAACTCACCT GCACTCTACC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGATTATGAC
    CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACGGTCATAG ACCTGGAGAA CAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG
    TGGAGAGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGATC
    AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGACTA CACCATTGAG
    GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CAGTGGAGGA ACGCTTAGCC
    CTGCATGTCC TTCAGCAACA AGGCTTGGTT CCTGAGCATG TGGAGTCACG GCCTCTTTAT
    AGTCCTCTGC AGCCAGATAT CGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA
    AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TAGAAGGTTT
    TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGTCTCACG
    GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAG
    CAGAAGACAG ATGTGCACTA CCGCTCCCTG GGTGGGGAGG GCAATTTCAA CTGGAGGTTC
    GTGTTTCCCT TCGACTATCT GCCTGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCC
    TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATCTGG
    GACAATGACA AATTCTCTTT TGATGACTTT CTGGGCTCTC TGCAGCTGGA TCTCAACCGA
    ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC
    CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT
    GTGACAGAGG AGGGCGAGAA GAAGATGTTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT
    GTTGCAGAAA GTGAACATGA AGAGCGGCCT GCTGGCCAAG GTCGGGATGA ACCCAACATG
    AATCCGAAGC TAGAGGATCC AAGGCGCCCC GATACTTCTT TCCTGTGGTT CACCTCCCCG
    TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGCTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506240.1
    CDS601..6870
    Translation

    Target ORF information:

    RefSeq Version XM_006506177.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506177.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGTGGA CGACAACCTG
    GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC
    ACGGGCTTCC CAGATCCTTA TGCAGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCTTAT
    CGAGGCAGGG TTCTGTTGTC CCTGGAGACC AAACTGGTGG AACACAGTGA GCAGAAGGTG
    GAAGACCTTC CTGCTGATGA TATCCTGCGT GTGGAGAAGT ACCTGCGGAG GCGCAAATAT
    TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA CGCCATCCAG
    TTCGAGGTCA GCATTGGGAA CTACGGGAAC AAGTTCGACA CCACCTGCCT GCCCTTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTATTA CTTGCCTTGG
    GGCAACGTGA AGCCCGTGGT GGTGCTGTCC TCATACTGGG AAGACATCAG CCATCGAATT
    GAGATCCAAA ACCAGCTCCT CAGGGTCGCT GACCGCCTGG AAGCTAACCT GGAGCAGGTC
    CACTTGGCTC TGAAGGCACA GTGTTCCTCC GAGGATGTGG ACGCCCTGGT GGCTCAGTTG
    ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCACGA GATACCTTCT
    GCCACCCACC TTGATCAGTA CCTGCTCCGG CTACGCACCC GTCACCTGAG CCAGATCAAA
    GAAGCTGCTC TGGCCTTGAA GCTTGGCCAT AGTGAGCTCT CCACTGCCCT GGAGCAGGCC
    GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCCGAGGAGC CCCAGAACAG CCTGCCGGAC
    ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAGCGGGT GCCTGCCCAT
    GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAAGCTGCAG
    ACCATCTTTC TGAAATATCC TATGGAAGGT ATGCCTGGGG CCCGAATGCC CGTGCAGATT
    CGGATCAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AATTCAATCA GTTTGCTGAG
    GGAAAGCTCT CTGTCTTTGC CGAAACGTAT GAGAACCAGA CAAAGCTGGC ACTGGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ATGTCACAGG CAAGATCAAA
    CTACCAAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA TTGGTTCGTG
    TGTCCAGAGA AGACCCTACT CCATGATGCT GACGCTGGTC ATCTGAGCTT CGTGGAGGAG
    GTGTTCGAGA ACCAGACCCG GCTCCCTGGG GGCCAGTGGA TTTACATGAG TGATAACTAC
    ACAGATGTGA ATGGGGAGAA GGTACTTCCG AAGGATGATA TTGAATGCCC GCTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG
    GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCAAAAC ACTGGGTCCC TGTTGAGAAG
    ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAAGCAC TGAAGAGGCA CAGGCAGGCA GAGGCCGAGG GCGAGGGCTG GGAGTATGCC
    TCTCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGAC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ACTCCATGTC TGTTTCTACC
    CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC
    CATCTGCGCT GCTACCTGTA CCAGGCCCGA GACCTGCCAG CCATGGACAA GGACTCCTTC
    TCTGATCCCT ACGCCATCGT CTCCTTCCTG CATCAAAGCC AGAAGACAGT GGTGGAGAAG
    AACACCCTGA ATCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATTGA GATCTTCGGC
    GAGCCAGCCA GTATCGCCGA GCATCCGCCC TGCATCGTGG TGGAGCTCTA TGACCACGAC
    ACCTATGGTG CTGACGAGTT CATGGGTCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG
    CCCCGCCTGG CCTGGTTCCC ACTGACCCGG GGCAGCCAGC CTGCCGGCGA GCTGCTGGCA
    GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG
    CATGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ATTTGCCTTA CCCACCACCT
    CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATTA AGCCAGCTCT CCAACGTACT
    GCCATTGAGA TCCTGGCCTG GGGCCTGCGG AACATGAAGA GCTATCAGAT GGCCAGCATC
    TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA
    AACCTTCGGA AGAACCCCAA CTTTGATGTC TGTACTCTCT TCATGGAAGT GATGTTACCC
    AGAGAGGACC TCTACTGTCC ACCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC
    CGCCGGCCAG TTGTGGGTCA GTGTACCATT CGCTCCCTGG AGAACTTCCT GTGCGACCCA
    TACTCAGCAG AGAGTCCATC CCCACAGGGT GGCCCAGATG ATGTGAGCTT ACTCAGCCCC
    GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCTC TCATTCCTGT CCAGCTTACA
    GATGGGCTGT CAAGCTTGGG CCCCACTAAC CTGACGCCTT CTCCATCCAG TCCTCATGAG
    GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTG TCGGGGAGAG GGAAAAGTGT
    GGTTCCTACC TGGAGAAGGA TTTTGACACC TTGAAGGTCT ATGACACACA GCTGGAGAAT
    GTGGAGGCTT TTGGAGGCCT GTCCGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAGG
    ACTCAGGAGG AGACAGATGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT
    TATCCCCTCC CAGAAGATCC AGCCATCCCC ATGCCCCCGA GACAATTCCA TCAGCTGGCT
    GCTCAGGGTC CCCAGGAATG CTTGGTCCGT ATTTATATCG TCCGAGCATT TGGCTTACAG
    CCCAAGGACC CCAATGGCAA GTGTGACCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATCCCTTGC ACCCTGGAGC CTGTATTCGG AAAGATGTTT
    GAACTCACCT GCACTCTACC TCTGGAGAAG GACCTGAAGA TCACACTCTA CGATTATGAC
    CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACGGTCATAG ACCTGGAGAA CAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG TGGGCTCCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG
    TGGAGAGATC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGATC
    AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGACTA CACCATTGAG
    GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CAGTGGAGGA ACGCTTAGCC
    CTGCATGTCC TTCAGCAACA AGGCTTGGTT CCTGAGCATG TGGAGTCACG GCCTCTTTAT
    AGTCCTCTGC AGCCAGATAT CGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA
    AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TAGAAGGTTT
    TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGTCTCACG
    GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAG
    CAGAAGACAG ATGTGCACTA CCGCTCCCTG GGTGGGGAGG GCAATTTCAA CTGGAGGTTC
    GTGTTTCCCT TCGACTATCT GCCTGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCC
    TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATCTGG
    GACAATGACA AATTCTCTTT TGATGACTTT CTGGGCTCTC TGCAGCTGGA TCTCAACCGA
    ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC
    CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT
    GTGACAGAGG AGGGCGAGAA GAAGATGTTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT
    GTTGCAGAAA GTGAACATGA AGAGCGGCCT GCTGGCCAAG GTCGGGATGA ACCCAACATG
    AATCCGAAGC TAGAGGATCC AAGGCGCCCC GATACTTCTT TCCTGTGGTT CACCTCCCCG
    TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGCTTCCGGT GTGCCATCAT CCTCTTCATC
    ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37306
    Clone ID Related Accession (Same CDS sequence) XM_006506178.2
    Accession Version XM_006506178.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTACTCA AGAGTCCACA AGCCACTGAC CTGGACGACA ACCTGGGCTT CCTCCCCACT
    TTTGGGCCCT GCTATGTGAA CCTCTATGGC AGTCCCCGAG AGTTCACGGG CTTCCCAGAT
    CCTTATGCAG AGCTCAACAC AGGAAAGGGA GAAGGTGTGG CTTATCGAGG CAGGGTTCTG
    TTGTCCCTGG AGACCAAACT GGTGGAACAC AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT
    GATGATATCC TGCGTGTGGA GAAGTACCTG CGGAGGCGCA AATATTCCCT CTTTGCTGCC
    TTCTACTCAG CCACAATGCT GCAGGATGTG GATGACGCCA TCCAGTTCGA GGTCAGCATT
    GGGAACTACG GGAACAAGTT CGACACCACC TGCCTGCCCT TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGATGG ATGCCACTAC TATTACTTGC CTTGGGGCAA CGTGAAGCCC
    GTGGTGGTGC TGTCCTCATA CTGGGAAGAC ATCAGCCATC GAATTGAGAT CCAAAACCAG
    CTCCTCAGGG TCGCTGACCG CCTGGAAGCT AACCTGGAGC AGGTCCACTT GGCTCTGAAG
    GCACAGTGTT CCTCCGAGGA TGTGGACGCC CTGGTGGCTC AGTTGACAGA TGAGCTCCTT
    GCAGACTGCA GCCAGCCCCT GTGTGACATC CACGAGATAC CTTCTGCCAC CCACCTTGAT
    CAGTACCTGC TCCGGCTACG CACCCGTCAC CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC
    TTGAAGCTTG GCCATAGTGA GCTCTCCACT GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG
    CATCTCCGTG CCCTGGCCGA GGAGCCCCAG AACAGCCTGC CGGACATCAT CATCTGGATG
    CTGCAGGGTG ACAAGCGTGT GGCCTACCAG CGGGTGCCTG CCCATGAAGT CCTCTTCTCC
    CGGCGGGGCC CCAGCTACTG CGGCAGGAAC TGTGGGAAGC TGCAGACCAT CTTTCTGAAA
    TATCCTATGG AAGGTATGCC TGGGGCCCGA ATGCCCGTGC AGATTCGGAT CAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAATTC AATCAGTTTG CTGAGGGAAA GCTCTCTGTC
    TTTGCCGAAA CGTATGAGAA CCAGACAAAG CTGGCACTGG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACAGGCAAGA TCAAACTACC AAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA GGAGATTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ATGCTGACGC TGGTCATCTG AGCTTCGTGG AGGAGGTGTT CGAGAACCAG
    ACCCGGCTCC CTGGGGGCCA GTGGATTTAC ATGAGTGATA ACTACACAGA TGTGAATGGG
    GAGAAGGTAC TTCCGAAGGA TGATATTGAA TGCCCGCTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCTGTT GATGAACAAG GCTGGGAGTA CAGCATCACC
    ATACCCCCAG ACCGAAAGCC AAAACACTGG GTCCCTGTTG AGAAGATGTA TTACACTCAC
    AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG AGGGACCTCA GCCAGATGGA AGCACTGAAG
    AGGCACAGGC AGGCAGAGGC CGAGGGCGAG GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACAGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCGC TGGAGAAGAC GGGACCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCATGGTGG ATGACAAGAG CGAAGACTCC ATGTCTGTTT CTACCCTGAG CTTTGGCGTT
    AACAGACCCA CAATCTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT GCGCTGCTAC
    CTGTACCAGG CCCGAGACCT GCCAGCCATG GACAAGGACT CCTTCTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCATCA AAGCCAGAAG ACAGTGGTGG AGAAGAACAC CCTGAATCCC
    ACCTGGGATC AAACCCTCAT CTTCTATGAG ATTGAGATCT TCGGCGAGCC AGCCAGTATC
    GCCGAGCATC CGCCCTGCAT CGTGGTGGAG CTCTATGACC ACGACACCTA TGGTGCTGAC
    GAGTTCATGG GTCGCTGTAT TTGTCAGCCA AGCCTGGAAC GGATGCCCCG CCTGGCCTGG
    TTCCCACTGA CCCGGGGCAG CCAGCCTGCC GGCGAGCTGC TGGCAGCGTT CGAGCTTATC
    CAGAGAGAGA AGCCAGCCAT CCATCACATC CCTGGCTTTG AGATGCATGA AACTTCAAGG
    ATTTTGGATG AGACGGAGGA CACAGATTTG CCTTACCCAC CACCTCAAAG GGAGGCCAAC
    ATCTACATGG TTCCCCAGAA CATTAAGCCA GCTCTCCAAC GTACTGCCAT TGAGATCCTG
    GCCTGGGGCC TGCGGAACAT GAAGAGCTAT CAGATGGCCA GCATCTCTTC CCCCAGCCTC
    GTGGTGGAGT GTGGTGGGCA GACAGTGCAG TCCTGTGTCA TCAGAAACCT TCGGAAGAAC
    CCCAACTTTG ATGTCTGTAC TCTCTTCATG GAAGTGATGT TACCCAGAGA GGACCTCTAC
    TGTCCACCTA TTGTGGTCAA GGTCATTGAT AATCGCCAGT TTGGCCGCCG GCCAGTTGTG
    GGTCAGTGTA CCATTCGCTC CCTGGAGAAC TTCCTGTGCG ACCCATACTC AGCAGAGAGT
    CCATCCCCAC AGGGTGGCCC AGATGATGTG AGCTTACTCA GCCCCGGGGA AGATGTGCTG
    ATTGACATCG ATGACAAGGA GCCTCTCATT CCTGTCCAGG AGGAGGAGTT CATTGACTGG
    TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG
    GATTTTGACA CCTTGAAGGT CTATGACACA CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC
    CTGTCCGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA GGACTCAGGA GGAGACAGAT
    GATCCATCTG TCATCGGAGA ATTTAAGGGT CTCTTCAAAA TTTATCCCCT CCCAGAAGAT
    CCAGCCATCC CCATGCCCCC GAGACAATTC CATCAGCTGG CTGCTCAGGG TCCCCAGGAA
    TGCTTGGTCC GTATTTATAT CGTCCGAGCA TTTGGCTTAC AGCCCAAGGA CCCCAATGGC
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATCCCTT GCACCCTGGA GCCTGTATTC GGAAAGATGT TTGAACTCAC CTGCACTCTA
    CCTCTGGAGA AGGACCTGAA GATCACACTC TACGATTATG ACCTGCTCTC CAAGGATGAG
    AAGATTGGGG AGACGGTCAT AGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGTGGGCTCC CACAGACCTA CTGTGTCTCT GGACCAAATC AGTGGAGAGA TCAGCTCCGC
    CCCTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCACAGGA TCAAGGCCCC CGTGTACCGG
    ACAGACCGAG TGACGTTTCA GGATAAGGAC TACACCATTG AGGAGATAGA GGCTGGCAGA
    CTCCCAAACC CACACCTGGG CCCAGTGGAG GAACGCTTAG CCCTGCATGT CCTTCAGCAA
    CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT
    ATCGAGCAGG GGAAGCTACA GATGTGGATT GACATATTTC CAAAGGTGCT GGGCCGGCCT
    GGACCTCCCT TCAACATCAC CCCACGGAAA GCTAGAAGGT TTTTTCTGCG TTGTATTATT
    TGGAACACAA AAGATGTGAT CTTGGATGAC CTCAGTCTCA CGGGGGAGAA GATGAGTGAC
    ATCTATGTGA AAGGCTGGAT GGTGGGATTC GAAGAACACA AGCAGAAGAC AGATGTGCAC
    TACCGCTCCC TGGGTGGGGA GGGCAATTTC AACTGGAGGT TCGTGTTTCC CTTCGACTAT
    CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC AAGAAGGATG CCTTCTGGAG ACTCGACAAG
    ACAGAGAGCA AGATCCCAGC ACGTGTGGTC TTCCAGATCT GGGACAATGA CAAATTCTCT
    TTTGATGACT TTCTGGGCTC TCTGCAGCTG GATCTCAACC GAATGCCCAA GCCAGCCAAG
    ACGGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACCT TCCACCCAGA ATGGTTTGTG
    TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA TGGTGGCCTT GTGTGACAGA GGAGGGCGAG
    AAGAAGATGT TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TTGTTGCAGA AAGTGAACAT
    GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT GAACCCAACA TGAATCCGAA GCTAGAGGAT
    CCAAGGCGCC CCGATACTTC TTTCCTGTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC
    ATCTTGTGGC GACGCTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTCCTG
    CTCTTCCTGG GTGTCTTTGT CTACGCCTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506241.1
    CDS255..6506
    Translation

    Target ORF information:

    RefSeq Version XM_006506178.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506178.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTCTGTT GCTTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
    AGCGATCCAG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG
    AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA
    GATCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG AAGAAACAGG
    TTCCTGGGGG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTCTCTGCC
    AGCTTCAACG CACCTCTGCT AGACGCCAAG CAGCAACCCA CGGGGGCCTC TCTGGTCCTG
    CAGGTGTCCT ACACGCCACC CCCAGGAGCT GTGCCCCTGT TCCCACCACC TGCTTCTCTA
    GCACCCTCCC CGACACTGCC TGACATGGAT CTGGTGCCTG ACACAGGAGG GGAGGAAGAC
    ACCGAGGACC AGGGCCTGAC TGGAGATGAA GCTGAGCCGT TCCTGGACCA GAGTGCTGCT
    GTGGGCCCAG GGGGACCGAC CACACCCAGG AAGCCACCTT CCCACCCTCC TCCCCACTAT
    CCAGGGGCAA AAAGAAAAAG AAGCTCTGCC CCACCTAGAA AGCTGCTGTC AGACAAGCCA
    CAGGACTTCC AGATCAGAGT CCAGGTGATA GAGGGGCGTC AGCTGCCTGG GGTGAATATT
    AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GAACTCGGAT CCAGAAGGGA
    AATAGCCCAC TCTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAGCTG
    TTTGATGAGC CCATCTTCAT CACGGTGGTG GACTCACGCT CCCTTAGGAC AGATGCCCTC
    CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA CGCTTATCTC
    CGGAAGTGGC TGCTGCTCTC CGACCCTGAT GACTTCTCTG CGGGGGCCAG AGGCTACCTC
    AAAGCCAGCC TGTGTGTGCT AGGGCCTGGA GATGAAGCTC CCCTGGACAA GAAGGACCCT
    TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACCGGTGT GGCCCTTCGA
    GGAGCCCACT TCTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT
    GTGATGGACA ACGTCAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA CTTGGTGGAT
    CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCTAACCCTC AGTGGAACCA GAACATCACC TTGCCTGCGA TGTTTCCCTC TATGTGTGAA
    AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCCACC
    ACCTACTTGG GTATGTCGAA AATCTCTGCT ACTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTACTCA AGAGTCCACA AGCCACTGAC CTGGACGACA ACCTGGGCTT CCTCCCCACT
    TTTGGGCCCT GCTATGTGAA CCTCTATGGC AGTCCCCGAG AGTTCACGGG CTTCCCAGAT
    CCTTATGCAG AGCTCAACAC AGGAAAGGGA GAAGGTGTGG CTTATCGAGG CAGGGTTCTG
    TTGTCCCTGG AGACCAAACT GGTGGAACAC AGTGAGCAGA AGGTGGAAGA CCTTCCTGCT
    GATGATATCC TGCGTGTGGA GAAGTACCTG CGGAGGCGCA AATATTCCCT CTTTGCTGCC
    TTCTACTCAG CCACAATGCT GCAGGATGTG GATGACGCCA TCCAGTTCGA GGTCAGCATT
    GGGAACTACG GGAACAAGTT CGACACCACC TGCCTGCCCT TGGCCTCCAC CACCCAGTAC
    AGCCGGGCGG TCTTTGATGG ATGCCACTAC TATTACTTGC CTTGGGGCAA CGTGAAGCCC
    GTGGTGGTGC TGTCCTCATA CTGGGAAGAC ATCAGCCATC GAATTGAGAT CCAAAACCAG
    CTCCTCAGGG TCGCTGACCG CCTGGAAGCT AACCTGGAGC AGGTCCACTT GGCTCTGAAG
    GCACAGTGTT CCTCCGAGGA TGTGGACGCC CTGGTGGCTC AGTTGACAGA TGAGCTCCTT
    GCAGACTGCA GCCAGCCCCT GTGTGACATC CACGAGATAC CTTCTGCCAC CCACCTTGAT
    CAGTACCTGC TCCGGCTACG CACCCGTCAC CTGAGCCAGA TCAAAGAAGC TGCTCTGGCC
    TTGAAGCTTG GCCATAGTGA GCTCTCCACT GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG
    CATCTCCGTG CCCTGGCCGA GGAGCCCCAG AACAGCCTGC CGGACATCAT CATCTGGATG
    CTGCAGGGTG ACAAGCGTGT GGCCTACCAG CGGGTGCCTG CCCATGAAGT CCTCTTCTCC
    CGGCGGGGCC CCAGCTACTG CGGCAGGAAC TGTGGGAAGC TGCAGACCAT CTTTCTGAAA
    TATCCTATGG AAGGTATGCC TGGGGCCCGA ATGCCCGTGC AGATTCGGAT CAAGCTCTGG
    TTCGGGCTCT CTGTGGATGA GAAGGAATTC AATCAGTTTG CTGAGGGAAA GCTCTCTGTC
    TTTGCCGAAA CGTATGAGAA CCAGACAAAG CTGGCACTGG TCGGGAACTG GGGCACAACG
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACAGGCAAGA TCAAACTACC AAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA GGAGATTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ATGCTGACGC TGGTCATCTG AGCTTCGTGG AGGAGGTGTT CGAGAACCAG
    ACCCGGCTCC CTGGGGGCCA GTGGATTTAC ATGAGTGATA ACTACACAGA TGTGAATGGG
    GAGAAGGTAC TTCCGAAGGA TGATATTGAA TGCCCGCTGG GCTGGAAGTG GGAAGATGAA
    GAATGGTCCA CAGACCTCAA TCGGGCTGTT GATGAACAAG GCTGGGAGTA CAGCATCACC
    ATACCCCCAG ACCGAAAGCC AAAACACTGG GTCCCTGTTG AGAAGATGTA TTACACTCAC
    AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG AGGGACCTCA GCCAGATGGA AGCACTGAAG
    AGGCACAGGC AGGCAGAGGC CGAGGGCGAG GGCTGGGAGT ATGCCTCTCT CTTCGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACAGAT GCCTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAGCCGC TGGAGAAGAC GGGACCTGCA GCTGTGTTTG CCCTCGAGGG GGCCCTGGGC
    GGCATGGTGG ATGACAAGAG CGAAGACTCC ATGTCTGTTT CTACCCTGAG CTTTGGCGTT
    AACAGACCCA CAATCTCCTG CATCTTTGAC TATGGGAACC GCTACCATCT GCGCTGCTAC
    CTGTACCAGG CCCGAGACCT GCCAGCCATG GACAAGGACT CCTTCTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCATCA AAGCCAGAAG ACAGTGGTGG AGAAGAACAC CCTGAATCCC
    ACCTGGGATC AAACCCTCAT CTTCTATGAG ATTGAGATCT TCGGCGAGCC AGCCAGTATC
    GCCGAGCATC CGCCCTGCAT CGTGGTGGAG CTCTATGACC ACGACACCTA TGGTGCTGAC
    GAGTTCATGG GTCGCTGTAT TTGTCAGCCA AGCCTGGAAC GGATGCCCCG CCTGGCCTGG
    TTCCCACTGA CCCGGGGCAG CCAGCCTGCC GGCGAGCTGC TGGCAGCGTT CGAGCTTATC
    CAGAGAGAGA AGCCAGCCAT CCATCACATC CCTGGCTTTG AGATGCATGA AACTTCAAGG
    ATTTTGGATG AGACGGAGGA CACAGATTTG CCTTACCCAC CACCTCAAAG GGAGGCCAAC
    ATCTACATGG TTCCCCAGAA CATTAAGCCA GCTCTCCAAC GTACTGCCAT TGAGATCCTG
    GCCTGGGGCC TGCGGAACAT GAAGAGCTAT CAGATGGCCA GCATCTCTTC CCCCAGCCTC
    GTGGTGGAGT GTGGTGGGCA GACAGTGCAG TCCTGTGTCA TCAGAAACCT TCGGAAGAAC
    CCCAACTTTG ATGTCTGTAC TCTCTTCATG GAAGTGATGT TACCCAGAGA GGACCTCTAC
    TGTCCACCTA TTGTGGTCAA GGTCATTGAT AATCGCCAGT TTGGCCGCCG GCCAGTTGTG
    GGTCAGTGTA CCATTCGCTC CCTGGAGAAC TTCCTGTGCG ACCCATACTC AGCAGAGAGT
    CCATCCCCAC AGGGTGGCCC AGATGATGTG AGCTTACTCA GCCCCGGGGA AGATGTGCTG
    ATTGACATCG ATGACAAGGA GCCTCTCATT CCTGTCCAGG AGGAGGAGTT CATTGACTGG
    TGGAGCAAAT TCTTTGCTTC TGTCGGGGAG AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG
    GATTTTGACA CCTTGAAGGT CTATGACACA CAGCTGGAGA ATGTGGAGGC TTTTGGAGGC
    CTGTCCGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA GGACTCAGGA GGAGACAGAT
    GATCCATCTG TCATCGGAGA ATTTAAGGGT CTCTTCAAAA TTTATCCCCT CCCAGAAGAT
    CCAGCCATCC CCATGCCCCC GAGACAATTC CATCAGCTGG CTGCTCAGGG TCCCCAGGAA
    TGCTTGGTCC GTATTTATAT CGTCCGAGCA TTTGGCTTAC AGCCCAAGGA CCCCAATGGC
    AAGTGTGACC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATCCCTT GCACCCTGGA GCCTGTATTC GGAAAGATGT TTGAACTCAC CTGCACTCTA
    CCTCTGGAGA AGGACCTGAA GATCACACTC TACGATTATG ACCTGCTCTC CAAGGATGAG
    AAGATTGGGG AGACGGTCAT AGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGTGGGCTCC CACAGACCTA CTGTGTCTCT GGACCAAATC AGTGGAGAGA TCAGCTCCGC
    CCCTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCACAGGA TCAAGGCCCC CGTGTACCGG
    ACAGACCGAG TGACGTTTCA GGATAAGGAC TACACCATTG AGGAGATAGA GGCTGGCAGA
    CTCCCAAACC CACACCTGGG CCCAGTGGAG GAACGCTTAG CCCTGCATGT CCTTCAGCAA
    CAAGGCTTGG TTCCTGAGCA TGTGGAGTCA CGGCCTCTTT ATAGTCCTCT GCAGCCAGAT
    ATCGAGCAGG GGAAGCTACA GATGTGGATT GACATATTTC CAAAGGTGCT GGGCCGGCCT
    GGACCTCCCT TCAACATCAC CCCACGGAAA GCTAGAAGGT TTTTTCTGCG TTGTATTATT
    TGGAACACAA AAGATGTGAT CTTGGATGAC CTCAGTCTCA CGGGGGAGAA GATGAGTGAC
    ATCTATGTGA AAGGCTGGAT GGTGGGATTC GAAGAACACA AGCAGAAGAC AGATGTGCAC
    TACCGCTCCC TGGGTGGGGA GGGCAATTTC AACTGGAGGT TCGTGTTTCC CTTCGACTAT
    CTGCCTGCTG AGCAAGTCTG TGCTGTTGCC AAGAAGGATG CCTTCTGGAG ACTCGACAAG
    ACAGAGAGCA AGATCCCAGC ACGTGTGGTC TTCCAGATCT GGGACAATGA CAAATTCTCT
    TTTGATGACT TTCTGGGCTC TCTGCAGCTG GATCTCAACC GAATGCCCAA GCCAGCCAAG
    ACGGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACCT TCCACCCAGA ATGGTTTGTG
    TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA TGGTGGCCTT GTGTGACAGA GGAGGGCGAG
    AAGAAGATGT TGGCGGGCAA GCTGGAAATG ACCTTGGAGA TTGTTGCAGA AAGTGAACAT
    GAAGAGCGGC CTGCTGGCCA AGGTCGGGAT GAACCCAACA TGAATCCGAA GCTAGAGGAT
    CCAAGGCGCC CCGATACTTC TTTCCTGTGG TTCACCTCCC CGTACAAGAC CATGAAGTTC
    ATCTTGTGGC GACGCTTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTCCTG
    CTCTTCCTGG GTGTCTTTGT CTACGCCTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTTCGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37307
    Clone ID Related Accession (Same CDS sequence) XM_006506179.3 , XM_006506179.4
    Accession Version XM_006506179.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTACTCAAGA GTCCACAAGC CACTGACCTG GACGACAACC TGGGCTTCCT CCCCACTTTT
    GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT
    TATGCAGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCTT ATCGAGGCAG GGTTCTGTTG
    TCCCTGGAGA CCAAACTGGT GGAACACAGT GAGCAGAAGG TGGAAGACCT TCCTGCTGAT
    GATATCCTGC GTGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC
    TACTCAGCCA CAATGCTGCA GGATGTGGAT GACGCCATCC AGTTCGAGGT CAGCATTGGG
    AACTACGGGA ACAAGTTCGA CACCACCTGC CTGCCCTTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGATGGATG CCACTACTAT TACTTGCCTT GGGGCAACGT GAAGCCCGTG
    GTGGTGCTGT CCTCATACTG GGAAGACATC AGCCATCGAA TTGAGATCCA AAACCAGCTC
    CTCAGGGTCG CTGACCGCCT GGAAGCTAAC CTGGAGCAGG TCCACTTGGC TCTGAAGGCA
    CAGTGTTCCT CCGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA
    GACTGCAGCC AGCCCCTGTG TGACATCCAC GAGATACCTT CTGCCACCCA CCTTGATCAG
    TACCTGCTCC GGCTACGCAC CCGTCACCTG AGCCAGATCA AAGAAGCTGC TCTGGCCTTG
    AAGCTTGGCC ATAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT
    CTCCGTGCCC TGGCCGAGGA GCCCCAGAAC AGCCTGCCGG ACATCATCAT CTGGATGCTG
    CAGGGTGACA AGCGTGTGGC CTACCAGCGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG
    CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAAGCTGC AGACCATCTT TCTGAAATAT
    CCTATGGAAG GTATGCCTGG GGCCCGAATG CCCGTGCAGA TTCGGATCAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAATTCAAT CAGTTTGCTG AGGGAAAGCT CTCTGTCTTT
    GCCGAAACGT ATGAGAACCA GACAAAGCTG GCACTGGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGATGTCACA GGCAAGATCA AACTACCAAA GGATAGCTTC
    CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GATTGGTTCG TGTGTCCAGA GAAGACCCTA
    CTCCATGATG CTGACGCTGG TCATCTGAGC TTCGTGGAGG AGGTGTTCGA GAACCAGACC
    CGGCTCCCTG GGGGCCAGTG GATTTACATG AGTGATAACT ACACAGATGT GAATGGGGAG
    AAGGTACTTC CGAAGGATGA TATTGAATGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA
    CCCCCAGACC GAAAGCCAAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG
    CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAAGC ACTGAAGAGG
    CACAGGCAGG CAGAGGCCGA GGGCGAGGGC TGGGAGTATG CCTCTCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACAGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCGCTGG AGAAGACGGG ACCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    ATGGTGGATG ACAAGAGCGA AGACTCCATG TCTGTTTCTA CCCTGAGCTT TGGCGTTAAC
    AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG CTGCTACCTG
    TACCAGGCCC GAGACCTGCC AGCCATGGAC AAGGACTCCT TCTCTGATCC CTACGCCATC
    GTCTCCTTCC TGCATCAAAG CCAGAAGACA GTGGTGGAGA AGAACACCCT GAATCCCACC
    TGGGATCAAA CCCTCATCTT CTATGAGATT GAGATCTTCG GCGAGCCAGC CAGTATCGCC
    GAGCATCCGC CCTGCATCGT GGTGGAGCTC TATGACCACG ACACCTATGG TGCTGACGAG
    TTCATGGGTC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC
    CCACTGACCC GGGGCAGCCA GCCTGCCGGC GAGCTGCTGG CAGCGTTCGA GCTTATCCAG
    AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCATGAAAC TTCAAGGATT
    TTGGATGAGA CGGAGGACAC AGATTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC
    TACATGGTTC CCCAGAACAT TAAGCCAGCT CTCCAACGTA CTGCCATTGA GATCCTGGCC
    TGGGGCCTGC GGAACATGAA GAGCTATCAG ATGGCCAGCA TCTCTTCCCC CAGCCTCGTG
    GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC
    AACTTTGATG TCTGTACTCT CTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT
    CCACCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCCGGCC AGTTGTGGGT
    CAGTGTACCA TTCGCTCCCT GGAGAACTTC CTGTGCGACC CATACTCAGC AGAGAGTCCA
    TCCCCACAGG GTGGCCCAGA TGATGTGAGC TTACTCAGCC CCGGGGAAGA TGTGCTGATT
    GACATCGATG ACAAGGAGCC TCTCATTCCT GTCCAGGAGG AGGAGTTCAT TGACTGGTGG
    AGCAAATTCT TTGCTTCTGT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT
    TTTGACACCT TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCTTT TGGAGGCCTG
    TCCGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAGGA CTCAGGAGGA GACAGATGAT
    CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA
    GCCATCCCCA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC
    TTGGTCCGTA TTTATATCGT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG
    TGTGACCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATCCCTTGCA CCCTGGAGCC TGTATTCGGA AAGATGTTTG AACTCACCTG CACTCTACCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAC GATTATGACC TGCTCTCCAA GGATGAGAAG
    ATTGGGGAGA CGGTCATAGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT
    GGGCTCCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA GCTCCGCCCC
    TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGATCA AGGCCCCCGT GTACCGGACA
    GACCGAGTGA CGTTTCAGGA TAAGGACTAC ACCATTGAGG AGATAGAGGC TGGCAGACTC
    CCAAACCCAC ACCTGGGCCC AGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAGCAACAA
    GGCTTGGTTC CTGAGCATGT GGAGTCACGG CCTCTTTATA GTCCTCTGCA GCCAGATATC
    GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA
    CCTCCCTTCA ACATCACCCC ACGGAAAGCT AGAAGGTTTT TTCTGCGTTG TATTATTTGG
    AACACAAAAG ATGTGATCTT GGATGACCTC AGTCTCACGG GGGAGAAGAT GAGTGACATC
    TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAGC AGAAGACAGA TGTGCACTAC
    CGCTCCCTGG GTGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG
    CCTGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCCT TCTGGAGACT CGACAAGACA
    GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATCTGGG ACAATGACAA ATTCTCTTTT
    GATGACTTTC TGGGCTCTCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC
    CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGAGAAG
    AAGATGTTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TTGCAGAAAG TGAACATGAA
    GAGCGGCCTG CTGGCCAAGG TCGGGATGAA CCCAACATGA ATCCGAAGCT AGAGGATCCA
    AGGCGCCCCG ATACTTCTTT CCTGTGGTTC ACCTCCCCGT ACAAGACCAT GAAGTTCATC
    TTGTGGCGAC GCTTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC
    TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506242.1
    CDS601..6849
    Translation

    Target ORF information:

    RefSeq Version XM_006506179.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506179.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTACTCAAGA GTCCACAAGC CACTGACCTG GACGACAACC TGGGCTTCCT CCCCACTTTT
    GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT
    TATGCAGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCTT ATCGAGGCAG GGTTCTGTTG
    TCCCTGGAGA CCAAACTGGT GGAACACAGT GAGCAGAAGG TGGAAGACCT TCCTGCTGAT
    GATATCCTGC GTGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC
    TACTCAGCCA CAATGCTGCA GGATGTGGAT GACGCCATCC AGTTCGAGGT CAGCATTGGG
    AACTACGGGA ACAAGTTCGA CACCACCTGC CTGCCCTTGG CCTCCACCAC CCAGTACAGC
    CGGGCGGTCT TTGATGGATG CCACTACTAT TACTTGCCTT GGGGCAACGT GAAGCCCGTG
    GTGGTGCTGT CCTCATACTG GGAAGACATC AGCCATCGAA TTGAGATCCA AAACCAGCTC
    CTCAGGGTCG CTGACCGCCT GGAAGCTAAC CTGGAGCAGG TCCACTTGGC TCTGAAGGCA
    CAGTGTTCCT CCGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA
    GACTGCAGCC AGCCCCTGTG TGACATCCAC GAGATACCTT CTGCCACCCA CCTTGATCAG
    TACCTGCTCC GGCTACGCAC CCGTCACCTG AGCCAGATCA AAGAAGCTGC TCTGGCCTTG
    AAGCTTGGCC ATAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT
    CTCCGTGCCC TGGCCGAGGA GCCCCAGAAC AGCCTGCCGG ACATCATCAT CTGGATGCTG
    CAGGGTGACA AGCGTGTGGC CTACCAGCGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG
    CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAAGCTGC AGACCATCTT TCTGAAATAT
    CCTATGGAAG GTATGCCTGG GGCCCGAATG CCCGTGCAGA TTCGGATCAA GCTCTGGTTC
    GGGCTCTCTG TGGATGAGAA GGAATTCAAT CAGTTTGCTG AGGGAAAGCT CTCTGTCTTT
    GCCGAAACGT ATGAGAACCA GACAAAGCTG GCACTGGTCG GGAACTGGGG CACAACGGGC
    CTCACCTACC CCAAGTTTTC TGATGTCACA GGCAAGATCA AACTACCAAA GGATAGCTTC
    CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GATTGGTTCG TGTGTCCAGA GAAGACCCTA
    CTCCATGATG CTGACGCTGG TCATCTGAGC TTCGTGGAGG AGGTGTTCGA GAACCAGACC
    CGGCTCCCTG GGGGCCAGTG GATTTACATG AGTGATAACT ACACAGATGT GAATGGGGAG
    AAGGTACTTC CGAAGGATGA TATTGAATGC CCGCTGGGCT GGAAGTGGGA AGATGAAGAA
    TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA
    CCCCCAGACC GAAAGCCAAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG
    CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAAGC ACTGAAGAGG
    CACAGGCAGG CAGAGGCCGA GGGCGAGGGC TGGGAGTATG CCTCTCTCTT CGGCTGGAAG
    TTCCACCTGG AGTACCGCAA GACAGATGCC TTCCGCCGCC GCCGCTGGCG CCGCCGCATG
    GAGCCGCTGG AGAAGACGGG ACCTGCAGCT GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC
    ATGGTGGATG ACAAGAGCGA AGACTCCATG TCTGTTTCTA CCCTGAGCTT TGGCGTTAAC
    AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG CTGCTACCTG
    TACCAGGCCC GAGACCTGCC AGCCATGGAC AAGGACTCCT TCTCTGATCC CTACGCCATC
    GTCTCCTTCC TGCATCAAAG CCAGAAGACA GTGGTGGAGA AGAACACCCT GAATCCCACC
    TGGGATCAAA CCCTCATCTT CTATGAGATT GAGATCTTCG GCGAGCCAGC CAGTATCGCC
    GAGCATCCGC CCTGCATCGT GGTGGAGCTC TATGACCACG ACACCTATGG TGCTGACGAG
    TTCATGGGTC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC
    CCACTGACCC GGGGCAGCCA GCCTGCCGGC GAGCTGCTGG CAGCGTTCGA GCTTATCCAG
    AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCATGAAAC TTCAAGGATT
    TTGGATGAGA CGGAGGACAC AGATTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC
    TACATGGTTC CCCAGAACAT TAAGCCAGCT CTCCAACGTA CTGCCATTGA GATCCTGGCC
    TGGGGCCTGC GGAACATGAA GAGCTATCAG ATGGCCAGCA TCTCTTCCCC CAGCCTCGTG
    GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC
    AACTTTGATG TCTGTACTCT CTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT
    CCACCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCCGGCC AGTTGTGGGT
    CAGTGTACCA TTCGCTCCCT GGAGAACTTC CTGTGCGACC CATACTCAGC AGAGAGTCCA
    TCCCCACAGG GTGGCCCAGA TGATGTGAGC TTACTCAGCC CCGGGGAAGA TGTGCTGATT
    GACATCGATG ACAAGGAGCC TCTCATTCCT GTCCAGGAGG AGGAGTTCAT TGACTGGTGG
    AGCAAATTCT TTGCTTCTGT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT
    TTTGACACCT TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCTTT TGGAGGCCTG
    TCCGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAGGA CTCAGGAGGA GACAGATGAT
    CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA
    GCCATCCCCA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC
    TTGGTCCGTA TTTATATCGT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG
    TGTGACCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATCCCTTGCA CCCTGGAGCC TGTATTCGGA AAGATGTTTG AACTCACCTG CACTCTACCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAC GATTATGACC TGCTCTCCAA GGATGAGAAG
    ATTGGGGAGA CGGTCATAGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT
    GGGCTCCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA GCTCCGCCCC
    TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGATCA AGGCCCCCGT GTACCGGACA
    GACCGAGTGA CGTTTCAGGA TAAGGACTAC ACCATTGAGG AGATAGAGGC TGGCAGACTC
    CCAAACCCAC ACCTGGGCCC AGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAGCAACAA
    GGCTTGGTTC CTGAGCATGT GGAGTCACGG CCTCTTTATA GTCCTCTGCA GCCAGATATC
    GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA
    CCTCCCTTCA ACATCACCCC ACGGAAAGCT AGAAGGTTTT TTCTGCGTTG TATTATTTGG
    AACACAAAAG ATGTGATCTT GGATGACCTC AGTCTCACGG GGGAGAAGAT GAGTGACATC
    TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAGC AGAAGACAGA TGTGCACTAC
    CGCTCCCTGG GTGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG
    CCTGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCCT TCTGGAGACT CGACAAGACA
    GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATCTGGG ACAATGACAA ATTCTCTTTT
    GATGACTTTC TGGGCTCTCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC
    CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGAGAAG
    AAGATGTTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TTGCAGAAAG TGAACATGAA
    GAGCGGCCTG CTGGCCAAGG TCGGGATGAA CCCAACATGA ATCCGAAGCT AGAGGATCCA
    AGGCGCCCCG ATACTTCTTT CCTGTGGTTC ACCTCCCCGT ACAAGACCAT GAAGTTCATC
    TTGTGGCGAC GCTTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC
    TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTTCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37309
    Clone ID Related Accession (Same CDS sequence) XM_006506181.3 , XM_006506181.4
    Accession Version XM_006506181.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product dysferlin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGTGGA CGACAACCTG
    GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC
    ACGGGCTTCC CAGATCCTTA TGCAGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCTTAT
    CGAGGCAGGG TTCTGTTGTC CCTGGAGACC AAACTGGTGG AACACAGTGA GCAGAAGGTG
    GAAGACCTTC CTGCTGATGA TATCCTGCGT GTGGAGAAGT ACCTGCGGAG GCGCAAATAT
    TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA CGCCATCCAG
    TTCGAGGTCA GCATTGGGAA CTACGGGAAC AAGTTCGACA CCACCTGCCT GCCCTTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTATTA CTTGCCTTGG
    GGCAACGTGA AGCCCGTGGT GGTGCTGTCC TCATACTGGG AAGACATCAG CCATCGAATT
    GAGATCCAAA ACCAGCTCCT CAGGGTCGCT GACCGCCTGG AAGCTAACCT GGAGCAGGTC
    CACTTGGCTC TGAAGGCACA GTGTTCCTCC GAGGATGTGG ACGCCCTGGT GGCTCAGTTG
    ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCACGA GATACCTTCT
    GCCACCCACC TTGATCAGTA CCTGCTCCGG CTACGCACCC GTCACCTGAG CCAGATCAAA
    GAAGCTGCTC TGGCCTTGAA GCTTGGCCAT AGTGAGCTCT CCACTGCCCT GGAGCAGGCC
    GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCCGAGGAGC CCCAGAACAG CCTGCCGGAC
    ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAGCGGGT GCCTGCCCAT
    GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAAGCTGCAG
    ACCATCTTTC TGAAATATCC TATGGAAGGT ATGCCTGGGG CCCGAATGCC CGTGCAGATT
    CGGATCAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AATTCAATCA GTTTGCTGAG
    GGAAAGCTCT CTGTCTTTGC CGAAACGTAT GAGAACCAGA CAAAGCTGGC ACTGGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ATGTCACAGG CAAGATCAAA
    CTACCAAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA TTGGTTCGTG
    TGTCCAGAGA AGACCCTACT CCATGATGCT GACGCTGGTC ATCTGAGCTT CGTGGAGGAG
    GTGTTCGAGA ACCAGACCCG GCTCCCTGGG GGCCAGTGGA TTTACATGAG TGATAACTAC
    ACAGATGTGA ATGGGGAGAA GGTACTTCCG AAGGATGATA TTGAATGCCC GCTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG
    GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCAAAAC ACTGGGTCCC TGTTGAGAAG
    ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAAGCAC TGAAGAGGCA CAGGCAGGCA GAGGCCGAGG GCGAGGGCTG GGAGTATGCC
    TCTCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGAC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ACTCCATGTC TGTTTCTACC
    CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC
    CATCTGCGCT GCTACCTGTA CCAGGCCCGA GACCTGCCAG CCATGGACAA GGACTCCTTC
    TCTGATCCCT ACGCCATCGT CTCCTTCCTG CATCAAAGCC AGAAGACAGT GGTGGAGAAG
    AACACCCTGA ATCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATTGA GATCTTCGGC
    GAGCCAGCCA GTATCGCCGA GCATCCGCCC TGCATCGTGG TGGAGCTCTA TGACCACGAC
    ACCTATGGTG CTGACGAGTT CATGGGTCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG
    CCCCGCCTGG CCTGGTTCCC ACTGACCCGG GGCAGCCAGC CTGCCGGCGA GCTGCTGGCA
    GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG
    CATGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ATTTGCCTTA CCCACCACCT
    CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATTA AGCCAGCTCT CCAACGTACT
    GCCATTGAGA TCCTGGCCTG GGGCCTGCGG AACATGAAGA GCTATCAGAT GGCCAGCATC
    TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA
    AACCTTCGGA AGAACCCCAA CTTTGATGTC TGTACTCTCT TCATGGAAGT GATGTTACCC
    AGAGAGGACC TCTACTGTCC ACCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC
    CGCCGGCCAG TTGTGGGTCA GTGTACCATT CGCTCCCTGG AGAACTTCCT GTGCGACCCA
    TACTCAGCAG AGAGTCCATC CCCACAGGGT GGCCCAGATG ATGTGAGCTT ACTCAGCCCC
    GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCTC TCATTCCTGT CCAGGAGGAG
    GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTGTCG GGGAGAGGGA AAAGTGTGGT
    TCCTACCTGG AGAAGGATTT TGACACCTTG AAGGTCTATG ACACACAGCT GGAGAATGTG
    GAGGCTTTTG GAGGCCTGTC CGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAGGACT
    CAGGAGGAGA CAGATGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT
    CCCCTCCCAG AAGATCCAGC CATCCCCATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT
    CAGGGTCCCC AGGAATGCTT GGTCCGTATT TATATCGTCC GAGCATTTGG CTTACAGCCC
    AAGGACCCCA ATGGCAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG
    AGTGACCAGG ATAACTACAT CCCTTGCACC CTGGAGCCTG TATTCGGAAA GATGTTTGAA
    CTCACCTGCA CTCTACCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA TTATGACCTG
    CTCTCCAAGG ATGAGAAGAT TGGGGAGACG GTCATAGACC TGGAGAACAG GCTGCTGTCC
    AAGTTTGGGG CTCGCTGTGG GCTCCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG
    AGAGATCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGATCAAG
    GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGACTACAC CATTGAGGAG
    ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCAG TGGAGGAACG CTTAGCCCTG
    CATGTCCTTC AGCAACAAGG CTTGGTTCCT GAGCATGTGG AGTCACGGCC TCTTTATAGT
    CCTCTGCAGC CAGATATCGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG
    GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTAG AAGGTTTTTT
    CTGCGTTGTA TTATTTGGAA CACAAAAGAT GTGATCTTGG ATGACCTCAG TCTCACGGGG
    GAGAAGATGA GTGACATCTA TGTGAAAGGC TGGATGGTGG GATTCGAAGA ACACAAGCAG
    AAGACAGATG TGCACTACCG CTCCCTGGGT GGGGAGGGCA ATTTCAACTG GAGGTTCGTG
    TTTCCCTTCG ACTATCTGCC TGCTGAGCAA GTCTGTGCTG TTGCCAAGAA GGATGCCTTC
    TGGAGACTCG ACAAGACAGA GAGCAAGATC CCAGCACGTG TGGTCTTCCA GATCTGGGAC
    AATGACAAAT TCTCTTTTGA TGACTTTCTG GGCTCTCTGC AGCTGGATCT CAACCGAATG
    CCCAAGCCAG CCAAGACGGC TGAGAAGTGC TCCTTGGACC AGCTGGATGA CACCTTCCAC
    CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAGACAGTGA AAGGATGGTG GCCTTGTGTG
    ACAGAGGAGG GCGAGAAGAA GATGTTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTT
    GCAGAAAGTG AACATGAAGA GCGGCCTGCT GGCCAAGGTC GGGATGAACC CAACATGAAT
    CCGAAGCTAG AGGATCCAAG GCGCCCCGAT ACTTCTTTCC TGTGGTTCAC CTCCCCGTAC
    AAGACCATGA AGTTCATCTT GTGGCGACGC TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TCCTGCTCTT CCTGGGTGTC TTTGTCTACG CCTTCCCGAA CTACGCTGCC
    ATGAAGCTGG TGAAGCCCTT TCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506244.1
    CDS600..6806
    Translation

    Target ORF information:

    RefSeq Version XM_006506181.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus dysferlin (Dysf), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506181.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGAG TCTTCATCCT TTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC
    AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAT
    CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGAAG AAACAGGTTC
    CTGGGGGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT CTCTGCCAGC
    TTCAACGCAC CTCTGCTAGA CGCCAAGCAG CAACCCACGG GGGCCTCTCT GGTCCTGCAG
    GTGTCCTACA CGCCACCCCC AGGAGCTGTG CCCCTGTTCC CACCACCTGC TTCTCTAGCA
    CCCTCCCCGA CACTGCCTGA CATGGATCTG GTGCCTGACA CAGGAGGGGA GGAAGACACC
    GAGGACCAGG GCCTGACTGG AGATGAAGCT GAGCCGTTCC TGGACCAGAG TGCTGCTGTG
    GGCCCAGGGG GACCGACCAC ACCCAGGAAG CCACCTTCCC ACCCTCCTCC CCACTATCCA
    GGGGCAAAAA GAAAAAGAAG CTCTGCCCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG
    GACTTCCAGA TCAGAGTCCA GGTGATAGAG GGGCGTCAGC TGCCTGGGGT GAATATTAAG
    CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGAA CTCGGATCCA GAAGGGAAAT
    AGCCCACTCT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAGCTGTTT
    GATGAGCCCA TCTTCATCAC GGTGGTGGAC TCACGCTCCC TTAGGACAGA TGCCCTCCTT
    GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACACGC TTATCTCCGG
    AAGTGGCTGC TGCTCTCCGA CCCTGATGAC TTCTCTGCGG GGGCCAGAGG CTACCTCAAA
    GCCAGCCTGT GTGTGCTAGG GCCTGGAGAT GAAGCTCCCC TGGACAAGAA GGACCCTTCT
    GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CCGGTGTGGC CCTTCGAGGA
    GCCCACTTCT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG
    ATGGACAACG TCAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAACTT GGTGGATCCC
    TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCT
    AACCCTCAGT GGAACCAGAA CATCACCTTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA
    ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCCACCACC
    TACTTGGGTA TGTCGAAAAT CTCTGCTACT GGAGGAGAAA TAGAAGTGGA CGACAACCTG
    GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC
    ACGGGCTTCC CAGATCCTTA TGCAGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCTTAT
    CGAGGCAGGG TTCTGTTGTC CCTGGAGACC AAACTGGTGG AACACAGTGA GCAGAAGGTG
    GAAGACCTTC CTGCTGATGA TATCCTGCGT GTGGAGAAGT ACCTGCGGAG GCGCAAATAT
    TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA CGCCATCCAG
    TTCGAGGTCA GCATTGGGAA CTACGGGAAC AAGTTCGACA CCACCTGCCT GCCCTTGGCC
    TCCACCACCC AGTACAGCCG GGCGGTCTTT GATGGATGCC ACTACTATTA CTTGCCTTGG
    GGCAACGTGA AGCCCGTGGT GGTGCTGTCC TCATACTGGG AAGACATCAG CCATCGAATT
    GAGATCCAAA ACCAGCTCCT CAGGGTCGCT GACCGCCTGG AAGCTAACCT GGAGCAGGTC
    CACTTGGCTC TGAAGGCACA GTGTTCCTCC GAGGATGTGG ACGCCCTGGT GGCTCAGTTG
    ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCACGA GATACCTTCT
    GCCACCCACC TTGATCAGTA CCTGCTCCGG CTACGCACCC GTCACCTGAG CCAGATCAAA
    GAAGCTGCTC TGGCCTTGAA GCTTGGCCAT AGTGAGCTCT CCACTGCCCT GGAGCAGGCC
    GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCCGAGGAGC CCCAGAACAG CCTGCCGGAC
    ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAGCGGGT GCCTGCCCAT
    GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAAGCTGCAG
    ACCATCTTTC TGAAATATCC TATGGAAGGT ATGCCTGGGG CCCGAATGCC CGTGCAGATT
    CGGATCAAGC TCTGGTTCGG GCTCTCTGTG GATGAGAAGG AATTCAATCA GTTTGCTGAG
    GGAAAGCTCT CTGTCTTTGC CGAAACGTAT GAGAACCAGA CAAAGCTGGC ACTGGTCGGG
    AACTGGGGCA CAACGGGCCT CACCTACCCC AAGTTTTCTG ATGTCACAGG CAAGATCAAA
    CTACCAAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA TTGGTTCGTG
    TGTCCAGAGA AGACCCTACT CCATGATGCT GACGCTGGTC ATCTGAGCTT CGTGGAGGAG
    GTGTTCGAGA ACCAGACCCG GCTCCCTGGG GGCCAGTGGA TTTACATGAG TGATAACTAC
    ACAGATGTGA ATGGGGAGAA GGTACTTCCG AAGGATGATA TTGAATGCCC GCTGGGCTGG
    AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG
    GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCAAAAC ACTGGGTCCC TGTTGAGAAG
    ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG
    ATGGAAGCAC TGAAGAGGCA CAGGCAGGCA GAGGCCGAGG GCGAGGGCTG GGAGTATGCC
    TCTCTCTTCG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT CCGCCGCCGC
    CGCTGGCGCC GCCGCATGGA GCCGCTGGAG AAGACGGGAC CTGCAGCTGT GTTTGCCCTC
    GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ACTCCATGTC TGTTTCTACC
    CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC
    CATCTGCGCT GCTACCTGTA CCAGGCCCGA GACCTGCCAG CCATGGACAA GGACTCCTTC
    TCTGATCCCT ACGCCATCGT CTCCTTCCTG CATCAAAGCC AGAAGACAGT GGTGGAGAAG
    AACACCCTGA ATCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATTGA GATCTTCGGC
    GAGCCAGCCA GTATCGCCGA GCATCCGCCC TGCATCGTGG TGGAGCTCTA TGACCACGAC
    ACCTATGGTG CTGACGAGTT CATGGGTCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG
    CCCCGCCTGG CCTGGTTCCC ACTGACCCGG GGCAGCCAGC CTGCCGGCGA GCTGCTGGCA
    GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG
    CATGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ATTTGCCTTA CCCACCACCT
    CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATTA AGCCAGCTCT CCAACGTACT
    GCCATTGAGA TCCTGGCCTG GGGCCTGCGG AACATGAAGA GCTATCAGAT GGCCAGCATC
    TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA
    AACCTTCGGA AGAACCCCAA CTTTGATGTC TGTACTCTCT TCATGGAAGT GATGTTACCC
    AGAGAGGACC TCTACTGTCC ACCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC
    CGCCGGCCAG TTGTGGGTCA GTGTACCATT CGCTCCCTGG AGAACTTCCT GTGCGACCCA
    TACTCAGCAG AGAGTCCATC CCCACAGGGT GGCCCAGATG ATGTGAGCTT ACTCAGCCCC
    GGGGAAGATG TGCTGATTGA CATCGATGAC AAGGAGCCTC TCATTCCTGT CCAGGAGGAG
    GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTGTCG GGGAGAGGGA AAAGTGTGGT
    TCCTACCTGG AGAAGGATTT TGACACCTTG AAGGTCTATG ACACACAGCT GGAGAATGTG
    GAGGCTTTTG GAGGCCTGTC CGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAGGACT
    CAGGAGGAGA CAGATGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT
    CCCCTCCCAG AAGATCCAGC CATCCCCATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT
    CAGGGTCCCC AGGAATGCTT GGTCCGTATT TATATCGTCC GAGCATTTGG CTTACAGCCC
    AAGGACCCCA ATGGCAAGTG TGACCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG
    AGTGACCAGG ATAACTACAT CCCTTGCACC CTGGAGCCTG TATTCGGAAA GATGTTTGAA
    CTCACCTGCA CTCTACCTCT GGAGAAGGAC CTGAAGATCA CACTCTACGA TTATGACCTG
    CTCTCCAAGG ATGAGAAGAT TGGGGAGACG GTCATAGACC TGGAGAACAG GCTGCTGTCC
    AAGTTTGGGG CTCGCTGTGG GCTCCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG
    AGAGATCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGATCAAG
    GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGACTACAC CATTGAGGAG
    ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCAG TGGAGGAACG CTTAGCCCTG
    CATGTCCTTC AGCAACAAGG CTTGGTTCCT GAGCATGTGG AGTCACGGCC TCTTTATAGT
    CCTCTGCAGC CAGATATCGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG
    GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTAG AAGGTTTTTT
    CTGCGTTGTA TTATTTGGAA CACAAAAGAT GTGATCTTGG ATGACCTCAG TCTCACGGGG
    GAGAAGATGA GTGACATCTA TGTGAAAGGC TGGATGGTGG GATTCGAAGA ACACAAGCAG
    AAGACAGATG TGCACTACCG CTCCCTGGGT GGGGAGGGCA ATTTCAACTG GAGGTTCGTG
    TTTCCCTTCG ACTATCTGCC TGCTGAGCAA GTCTGTGCTG TTGCCAAGAA GGATGCCTTC
    TGGAGACTCG ACAAGACAGA GAGCAAGATC CCAGCACGTG TGGTCTTCCA GATCTGGGAC
    AATGACAAAT TCTCTTTTGA TGACTTTCTG GGCTCTCTGC AGCTGGATCT CAACCGAATG
    CCCAAGCCAG CCAAGACGGC TGAGAAGTGC TCCTTGGACC AGCTGGATGA CACCTTCCAC
    CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAGACAGTGA AAGGATGGTG GCCTTGTGTG
    ACAGAGGAGG GCGAGAAGAA GATGTTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTT
    GCAGAAAGTG AACATGAAGA GCGGCCTGCT GGCCAAGGTC GGGATGAACC CAACATGAAT
    CCGAAGCTAG AGGATCCAAG GCGCCCCGAT ACTTCTTTCC TGTGGTTCAC CTCCCCGTAC
    AAGACCATGA AGTTCATCTT GTGGCGACGC TTCCGGTGTG CCATCATCCT CTTCATCATC
    CTCTTCATCC TCCTGCTCTT CCTGGGTGTC TTTGTCTACG CCTTCCCGAA CTACGCTGCC
    ATGAAGCTGG TGAAGCCCTT TCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.