fer-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	fer-1
Entrez Gene ID	172659
Full Name	Sperm vesicle fusion protein fer-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001129005.3	NP_001122477.1	C2 domain-containing protein
NM_059936.3	NP_492337.1	Sperm vesicle fusion protein fer-1
NM_001129005.2	NP_001122477.1	Sperm vesicle fusion protein fer-1
NM_059936.4	NP_492337.1	Sperm vesicle fusion protein fer-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DYSF	NP_001124454.1
	DYSF	XP_002799336.1
Caenorhabditis elegans (roundworm)	fer-1	NP_492337.1
Caenorhabditis elegans (roundworm)	ferl-1	NP_491602.2
Rattus norvegicus (Norway rat)	Dysf	NP_001101339.1
Danio rerio (zebrafish)	dysf	NP_001244122.1
Mus musculus (house mouse)	Dysf	NP_067444.2
Canis lupus familiaris (dog)	DYSF	XP_003432278.1
Bos taurus (cattle)	DYSF	NP_001095960.1
Pan troglodytes (chimpanzee)	DYSF	XP_003309136.1
Gallus gallus (chicken)	DYSF	XP_423126.4

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that processes other than sarcomere stability may be affected by loss of fer-1 in C. elegans muscle.
Title: C. elegans dysferlin homolog fer-1 is expressed in muscle, and fer-1 mutations initiate altered gene expression of muscle enriched genes.

We found three FER-1 isoforms, two of which might arise from FER-1 by proteolysis. By both light and electron microscopy, these FER-1 proteins were found to be localized to membranous organelle membranes.
Title: FER-1 regulates Ca2+ -mediated membrane fusion during C. elegans spermatogenesis.

The following fer-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fer-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa350824	NM_001129005.3 Latest version!	Caenorhabditis elegans C2 domain-containing protein (fer-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa335673	NM_059936.4 Latest version!	Caenorhabditis elegans Sperm vesicle fusion protein fer-1 (fer-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa350824	NM_001129005.2	Caenorhabditis elegans Sperm vesicle fusion protein fer-1 (fer-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa335673	NM_059936.3	Caenorhabditis elegans Sperm vesicle fusion protein fer-1 (fer-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fer-1 ( NM_001129005.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001122477.1 Caenorhabditis elegans C2 domain-containing protein (fer-1), partial mRNA.

Price & Availability↑

CloneID	OCa350824
Clone ID Related Accession (Same CDS sequence)	NM_001129005.3 , NM_001129005.2
Accession Version	NM_001129005.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	C2 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001129005.2. COMPLETENESS: incomplete on the 5' end.

ATGACAAGAA CAGTTACTCA TGCAATTCCA AAGTGGCGAC AAAATATTCT CTACACAATA 
AAGAACCAGC CACTGGAGAA GCTTGCTGCC AAAGTTTTGA CTCTTCGGCT CGTGCGTCCT 
ACAGCACTTG GTGAATCAAG CGTCGGAGAG TTCAGCTGCT ACCTCTCGGA AGTAATTCAC 
TCTCCCGACA GATCTGTCAT TGCAAAATGG GTCGCTCTGG GATTTCCTGG AATGGATGAC 
GAGGAACCGG ACGATATCGC CTATGAGAAC TGTGGATTCC TGAAGGTGAC GCTCTCTGTG 
TACCGGATCG ACGAGTCACC ACCAACGCTG ATTGATGACG ATGGGAAGGA GCAAATCTGG 
AGTGGAGCAC ATCTCATTGA TTATACTCTT AAGATCAGAG TGTTCTCCGT GGAACAGCTC 
ATCCGACAGA TGATCAATGA GAGGAAAATC AAGAAGAAGC AAAGATTCTA TGTGATGGTT 
CAGTGTGGAG CACACAAGAC CGAAACAACA ATGGAATCTG CGTTTCTTGA TGAAGATGCA 
AACATCGCAT CAGTGAAGTT TGAGCAAGAG ATCTATCTTC CAATTCAATG GCCAACTGTC 
ATCTCAGAGA TTATTTTCTC ATTGTTCGAC AAAAAGGGAC GAGGCAAAAC GTGCATAGGA 
AAAGCCACTC TTCCAATGAA AATGATTTAC GAGCCAGGAG AGACTGGATA CCTTCCGACT 
TTTGGACCTG CGTTTCTCAA TGTATTCGAT TGCGAACGCG TGACTCGATT CAGCATCTTC 
TCAAGAAAAA TGCGAGGATC CCAGCAGGAT GGGAGCAGAT TTGTTGGGCG TTTGTTCATT 
GCGATCGATT GTGTAGAGTA TATGGAGAAG GCTAATGCTC AGATCATGCA TCTTGATCAT 
TCCCCAATTA TGGAAGCCGA ATCGATTTGT AAAAATCTCG AATGGTACAA CTGCTTCTGT 
TCCATGAGTG GCCTCAACAT GATCAATCCT CAATTCGCAT CCGATCCAGT CTGCATAATG 
ATGTCAATCG GTTCATTTGG AAGCACGAGC AACGAGTTCA GATCATGCAA TAGCTCAACA 
CTGCCAGCAG AGCCAAACTG GGATGGATGC AAGTACTTCA CGATGCCATG GGGAAATCTC 
CGACCAGTTG CCGAAGTCAA TGCTCCGTGG GAAGTTATTG AGCATCGGAT TGAAATGAGC 
AATGTTCTCA TGAAAATGAC GAATATGCTT GATACGATGA TTTGGGAGGT TCGACGCATT 
GGAAACAAAG CAATCGACCA TGTTTCGTCA GTTGGAATGG AAGCTTTGGA GTGCTTGGAG 
CAGCAAATTG ATTCGGCTAG CAAGTATTTG GGACGCATCA ACCCCGTTAG CAGCAACGCA 
CTGGATCGCC ATATCCTTGA CTACCGGAAG GAAAAGATCT TGAAGCTGCG AGAGCATTTT 
GAGAAGGAAG CCTTCAGTAT AGATTATTCT GATGGAGAAG TCGATGCGAA GCTGCTCCGA 
ATGCTTCTAA AAATTCGATC GATGACTCTT GAGTTGGCAG AAGATGTTCA AATGACCATT 
CCACCCGTTC TCATCAAAAT GATGTCCCAT GGGAAGTTGA TCGGTTTCGC CAAAATCCCG 
ATTTCGGAGA TTTTCCAATC GGATGATGAA GCTCAATCAG GAGAATGGTG TGGGAGGACC 
CGTGCTATAA ATATTCAATG GCCGACTTTG GTGGATCAGA GAAACAGGAA AAGAGAGCAC 
GTCGCAGTAC TCCATGCAAA GATGTGGTTT GGACGTACAG ATCAGCTCAC AAAATGGAAG 
GAGCACGTAC AACCCGCAGA CATCCGTCGT TTCATGGAAA TGTACGAAGT GCAGACGAAG 
GGACTCGCAT TGAAATGGAA AGATGAAGCT GATATTTACG ATGGGAAATG GGAGAAGACG 
AATGAAGTGC CTCTCGAAAA TGGATGGAAT GCTGTTGGTC ACTGGATTGT GATGAATACT 
CGTCATATGT TTGTACCGAA ACTTGGACAA CACACGGTTC ATGACAAGGC TTTTGAAATC 
CAGAAGAGAA CTGAAGACGG AACGTGGAAA CACTTTAAGT ACACGGATTG CTACGCCGAC 
GAGTTGAAAC CCAAGGATCT TGACAAACAT GCGAAAGGAT GGGAAGTAGG CTCTTGGGCT 
CAAGACAAGT TTCGAAACAA TGGAGACGAA AAAGGATGGG TGTACTCGAC GAATGGTGTC 
TTTTTTGGAA GCGGAGTCCT GACAGACCGA GAAGAAAAAG TTCACCACAA CTTCAGAAAG 
CGTTGTATCA AGAGGCCAAG GAAGCATGAA GGCTATAACA AGGAGTTGGA GGACTTTGAG 
CATTTTCGGA CAACGATGGG AAATGAGAAT TGGGAGTACT CCCCCAGCAA GAAGGAAGGC 
CCATACCACG ATTTGGAAGA CCGAACCGAT CGGATCAGAC GTAGACGATA TGTACGAGAA 
GTTGAGAACA AAGACCCGGA CAGTGAAGAT CCAAGATTTC GCGTTTACGA ATATCAAATG 
CAAACTGGAA AATGGCAACT GAGATGCTAT ATCATGTGGG GAAACGATTT GCTGCCAGTT 
GTCAAGAACT CGTCTCGGGC ATTTGTTCGC ATTTCGTTTG CACAATACTC AAAGCAGACG 
CTTCTCGTGG ACAACTCTCA AAATCCAATT TGGAATGAGA CAGTCATGTT CAAGTCAGTT 
CTGATCGCTG GTGGAACTCG AGATATCATG AAATACCCGC CAGTTGTCTC TGTTGAAGTC 
GTTGGCGAGT GCTCAAACAA TGAGGAGGCA AACCTCGGAC ACTTCGAAAC CAAACCAAAC 
GTCATTTGCG GAAATACGGA TGTTCGTGGC ACGCCACAAT GGTTTCCGTT GAAGTTCTCC 
AATGGACGTA CTCGTGGAGC GGTTCTTGCA TGCTTCGAGC TTTACTCAGA AGAAGATAAA 
GATTTGATTC CACTGGAGCC AAAATGCAAA CACAATTATA AGGAGAGAAG TGAGATCCCA 
ACAGAGTTCA GACCACAGTT CGATAAATTC CACGTGCAAT TTTTATGCTG GGGTGTGAGA 
AATCTCAAGA AGCATAAGCT TCTCGCCGTA CGTCGCCCAT TTGTTGGACT GACAATTGGG 
GATCAAGAGT TCACATTGCA ACCTCTCAAG GATGTCCGAA GAGATCCGAA CTTCCCTGAG 
CCCATGATTG TTTTTGGAGA AGTAATCCTA CCATCTGCTC TTGAGCTATC ACCTCCACTA 
ATCATCAATC TCTTCGATGC TAGGGCCTTC AATCGTCAGC CATTAGTTGG ATCATGCTTG 
GTATCTGATC TCCATAAATA CGTCTCACAT ATTGTTCCCA AGGTCAAAAG TGATCACGCC 
GAAAGATGGG AACAGTTGGA TTTGGTAATT ACGGAGGAGT TTGATCAGAT CATCAGGATG 
GTTCGTGTTC CGACTTTAAC AACTGATCCT ATGGTACCTT TGGATTGGTG GAGCAGATAT 
TATGCATCGA TGTCACAATT TCATAGATCA CCGGGATATC CAGAATCTGG CATGGAGTAT 
GTACGTATCT TCCGCCGACC ACTTGAACAG ATGAACGGAT ACAACAACTT CTCTGATTTC 
CTGGACACAT TCCCGTTTGT CAAATCAATG AAGGGAGATT TCGACGATCC GGAAGAAAAA 
GAAAAGGCTG GAGAATTGAA ATGCCGACTT CTCATCTCGA AACTCAAGAA GGGTAAGCCT 
CCAGCTGCGA TAAATACCGT TGTCGACTTC GTTGGACCAA CTCGATGTCT CGTCCGAGTT 
TACATCATCG AAGCAAATGG GCTGATCTCA AATGCACGGA AAGGACGAGT CGACTCGTAC 
GTTAAACTTC ATTGCGGAAA GCAGAACGTG AACCTGAAGA AGAACTACCG CTCAGAGTGT 
TGTGATCCGA TATTTGGAGA ACGAGTTGAC ATGACGGTTA CGATTCCGCT GGAGAAAGAT 
CTGAAAATCA CTGTGATGGG CAAGCGGAGA ATCCTTTCGG ATCAAGAAAT AGGATCAACT 
ACAATCGATC TGGAAAATCG TCTTCTCACA AAATGGAGAG CAACTGGTGG GCTGAGTGGT 
CAGTTCACTG TACAAGGAGA GCTGCAATGG AGGGATCAGA TGACTCCAAT GGAGATCCTG 
AAGTCGTACT GCTACAAGAT GATGCTATCA GTTCCAAAGA TTGAAAGTAG GCAGACGGAG 
AGAGGAGAAG AGAAGGGAAT TACCATTGAG AAGATCACAT TCTGGTTCAG TGAAGTTCTC 
CATGTCTTTG AGAACGAAGA GATTGCTATG CTGAATTCTC AACGTCAAAA AGCTGGAAAA 
GAAAATTTCA GTGATGGTTC TGATCAACAA AATGAGGACG TATCAGACGG TAGTTGGGAT 
GAAGAGGATC TAGAACGAGA GAAAGAGAAG CTAAAGTGGG AGAAGCATCG GAGCAAGGGC 
AAGCCATTGA AGAAGGTTAC AACGGAAAAG GCAGAAACGG CTGATGGAGA GTTGAGGAAG 
AAGGCGAAGA TGAGAATAAT GGGATCTCAG TTGCAAACAA TTGCTCTCTT CATTCTCCGA 
CAGATGAATT TGGTCCCAGA GCATGTCGAA TCTCGGCCAT TGTTCTCGGA TAAAGGAGGA 
AGAACTCAGA AGGGAGAACT TCGAATGTTC GTTGACATCT TTCCGATGGA ATATGGCGCG 
ATTCCTGCTC CATTCAACAT TGCACCACGG AAACCCATCA ACTATCAGCT ACGTATCGCG 
GTCATGGATG TACGAGGAGC GATTCCAGTC AAAAGAAGCT TTGCCGAGCC TGTCTCCGAT 
CTTTACGTCA AGGCATTCAT CAATGGAATG ACTAAGGGAC ACAAGACGGA TACGCATTTC 
AGAGTTCTCG ATGGAACCGG GGAGTTCAAC TGGCGTTTCC TGCTCAACTT CGACTACAAT 
CCTTGGGAGA AGAAGGTGGT CGCGTACACC AAAAACCGAT TCTTCTGCAA ACCAGTGGAA 
GAACTCGTCG ATCCGATTCT TGTCATTGAG CTTTGGGATA AGAACAAGTT CCGCAAGGAT 
CGTCTTCTCG GTGACATTGA ACTGGATCTT CTCGATTTCA TTGAGGGAAT CGGTTCTCCA 
TCCGACGTTG GTGTCTACTC AACAAAGAAG AGACAGCGTG GAGTGAAGTG TCCAAAGTGT 
TGTACCAGGA GAGGATGTCT CTGCAAATGC TGTATCTTCT GCTTTGAGAC CAAGTGCTTG 
TGTGGAAAAC GCAAAGTGAA GAAGAAGCCA TTCCCGAAGC CTGTGTTGTT CGTGGAACCA 
GAAGGTTACG ATGATACTGT GAACATCTTC GAATCTCGCA ACTTGTACGG TTGGTGGCCA 
ATGCTTACCG AGGAGTATCC ACACGAGGAA CCTCAGAATG CCAAGAAGAA GAATGATGAC 
GTTGGAAAGG ATCCTAAATG GATCATGGGT CTTGTTGAGA TGGACATGCT ACTTCTCACC 
AAACAAGAAG CCGATCAAGA GCCAGCTGGA AAGAAACGAT CCGAGCCGAA CCACAGCCCA 
TTCTTGGAAA AACCTGATCG GAAGTCATGG GCCAACTCGT GGTTGGTGTC CCGAATCAAG 
CCGTGCATCA AGTATTTCTG GCACTACTAT GGTCTTCAAA TTCTGCTCTG GCTCATCATT 
ATTGTTATTC TGATTCTCAC AATTTTCGTG TTGTTGCACA CTTGGCCGAC AATTTTAGCC 
GAAATTATAA AGGCAATTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122477.1
CDS	1..5724
Translation	MTRTVTHAIPKWRQNILYTIKNQPLEKLAAKVLTLRLVRPTALGESSVGEFSCYLSEVIHSPDRSVIAKWVALGFPGMDDEEPDDIAYENCGFLKVTLSVYRIDESPPTLIDDDGKEQIWSGAHLIDYTLKIRVFSVEQLIRQMINERKIKKKQRFYVMVQCGAHKTETTMESAFLDEDANIASVKFEQEIYLPIQWPTVISEIIFSLFDKKGRGKTCIGKATLPMKMIYEPGETGYLPTFGPAFLNVFDCERVTRFSIFSRKMRGSQQDGSRFVGRLFIAIDCVEYMEKANAQIMHLDHSPIMEAESICKNLEWYNCFCSMSGLNMINPQFASDPVCIMMSIGSFGSTSNEFRSCNSSTLPAEPNWDGCKYFTMPWGNLRPVAEVNAPWEVIEHRIEMSNVLMKMTNMLDTMIWEVRRIGNKAIDHVSSVGMEALECLEQQIDSASKYLGRINPVSSNALDRHILDYRKEKILKLREHFEKEAFSIDYSDGEVDAKLLRMLLKIRSMTLELAEDVQMTIPPVLIKMMSHGKLIGFAKIPISEIFQSDDEAQSGEWCGRTRAINIQWPTLVDQRNRKREHVAVLHAKMWFGRTDQLTKWKEHVQPADIRRFMEMYEVQTKGLALKWKDEADIYDGKWEKTNEVPLENGWNAVGHWIVMNTRHMFVPKLGQHTVHDKAFEIQKRTEDGTWKHFKYTDCYADELKPKDLDKHAKGWEVGSWAQDKFRNNGDEKGWVYSTNGVFFGSGVLTDREEKVHHNFRKRCIKRPRKHEGYNKELEDFEHFRTTMGNENWEYSPSKKEGPYHDLEDRTDRIRRRRYVREVENKDPDSEDPRFRVYEYQMQTGKWQLRCYIMWGNDLLPVVKNSSRAFVRISFAQYSKQTLLVDNSQNPIWNETVMFKSVLIAGGTRDIMKYPPVVSVEVVGECSNNEEANLGHFETKPNVICGNTDVRGTPQWFPLKFSNGRTRGAVLACFELYSEEDKDLIPLEPKCKHNYKERSEIPTEFRPQFDKFHVQFLCWGVRNLKKHKLLAVRRPFVGLTIGDQEFTLQPLKDVRRDPNFPEPMIVFGEVILPSALELSPPLIINLFDARAFNRQPLVGSCLVSDLHKYVSHIVPKVKSDHAERWEQLDLVITEEFDQIIRMVRVPTLTTDPMVPLDWWSRYYASMSQFHRSPGYPESGMEYVRIFRRPLEQMNGYNNFSDFLDTFPFVKSMKGDFDDPEEKEKAGELKCRLLISKLKKGKPPAAINTVVDFVGPTRCLVRVYIIEANGLISNARKGRVDSYVKLHCGKQNVNLKKNYRSECCDPIFGERVDMTVTIPLEKDLKITVMGKRRILSDQEIGSTTIDLENRLLTKWRATGGLSGQFTVQGELQWRDQMTPMEILKSYCYKMMLSVPKIESRQTERGEEKGITIEKITFWFSEVLHVFENEEIAMLNSQRQKAGKENFSDGSDQQNEDVSDGSWDEEDLEREKEKLKWEKHRSKGKPLKKVTTEKAETADGELRKKAKMRIMGSQLQTIALFILRQMNLVPEHVESRPLFSDKGGRTQKGELRMFVDIFPMEYGAIPAPFNIAPRKPINYQLRIAVMDVRGAIPVKRSFAEPVSDLYVKAFINGMTKGHKTDTHFRVLDGTGEFNWRFLLNFDYNPWEKKVVAYTKNRFFCKPVEELVDPILVIELWDKNKFRKDRLLGDIELDLLDFIEGIGSPSDVGVYSTKKRQRGVKCPKCCTRRGCLCKCCIFCFETKCLCGKRKVKKKPFPKPVLFVEPEGYDDTVNIFESRNLYGWWPMLTEEYPHEEPQNAKKKNDDVGKDPKWIMGLVEMDMLLLTKQEADQEPAGKKRSEPNHSPFLEKPDRKSWANSWLVSRIKPCIKYFWHYYGLQILLWLIIIVILILTIFVLLHTWPTILAEIIKAIF

Target ORF information:

RefSeq Version	NM_001129005.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans C2 domain-containing protein (fer-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129005.3

ORF Insert Sequence:

ATGACAAGAA CAGTTACTCA TGCAATTCCA AAGTGGCGAC AAAATATTCT CTACACAATA 
AAGAACCAGC CACTGGAGAA GCTTGCTGCC AAAGTTTTGA CTCTTCGGCT CGTGCGTCCT 
ACAGCACTTG GTGAATCAAG CGTCGGAGAG TTCAGCTGCT ACCTCTCGGA AGTAATTCAC 
TCTCCCGACA GATCTGTCAT TGCAAAATGG GTCGCTCTGG GATTTCCTGG AATGGATGAC 
GAGGAACCGG ACGATATCGC CTATGAGAAC TGTGGATTCC TGAAGGTGAC GCTCTCTGTG 
TACCGGATCG ACGAGTCACC ACCAACGCTG ATTGATGACG ATGGGAAGGA GCAAATCTGG 
AGTGGAGCAC ATCTCATTGA TTATACTCTT AAGATCAGAG TGTTCTCCGT GGAACAGCTC 
ATCCGACAGA TGATCAATGA GAGGAAAATC AAGAAGAAGC AAAGATTCTA TGTGATGGTT 
CAGTGTGGAG CACACAAGAC CGAAACAACA ATGGAATCTG CGTTTCTTGA TGAAGATGCA 
AACATCGCAT CAGTGAAGTT TGAGCAAGAG ATCTATCTTC CAATTCAATG GCCAACTGTC 
ATCTCAGAGA TTATTTTCTC ATTGTTCGAC AAAAAGGGAC GAGGCAAAAC GTGCATAGGA 
AAAGCCACTC TTCCAATGAA AATGATTTAC GAGCCAGGAG AGACTGGATA CCTTCCGACT 
TTTGGACCTG CGTTTCTCAA TGTATTCGAT TGCGAACGCG TGACTCGATT CAGCATCTTC 
TCAAGAAAAA TGCGAGGATC CCAGCAGGAT GGGAGCAGAT TTGTTGGGCG TTTGTTCATT 
GCGATCGATT GTGTAGAGTA TATGGAGAAG GCTAATGCTC AGATCATGCA TCTTGATCAT 
TCCCCAATTA TGGAAGCCGA ATCGATTTGT AAAAATCTCG AATGGTACAA CTGCTTCTGT 
TCCATGAGTG GCCTCAACAT GATCAATCCT CAATTCGCAT CCGATCCAGT CTGCATAATG 
ATGTCAATCG GTTCATTTGG AAGCACGAGC AACGAGTTCA GATCATGCAA TAGCTCAACA 
CTGCCAGCAG AGCCAAACTG GGATGGATGC AAGTACTTCA CGATGCCATG GGGAAATCTC 
CGACCAGTTG CCGAAGTCAA TGCTCCGTGG GAAGTTATTG AGCATCGGAT TGAAATGAGC 
AATGTTCTCA TGAAAATGAC GAATATGCTT GATACGATGA TTTGGGAGGT TCGACGCATT 
GGAAACAAAG CAATCGACCA TGTTTCGTCA GTTGGAATGG AAGCTTTGGA GTGCTTGGAG 
CAGCAAATTG ATTCGGCTAG CAAGTATTTG GGACGCATCA ACCCCGTTAG CAGCAACGCA 
CTGGATCGCC ATATCCTTGA CTACCGGAAG GAAAAGATCT TGAAGCTGCG AGAGCATTTT 
GAGAAGGAAG CCTTCAGTAT AGATTATTCT GATGGAGAAG TCGATGCGAA GCTGCTCCGA 
ATGCTTCTAA AAATTCGATC GATGACTCTT GAGTTGGCAG AAGATGTTCA AATGACCATT 
CCACCCGTTC TCATCAAAAT GATGTCCCAT GGGAAGTTGA TCGGTTTCGC CAAAATCCCG 
ATTTCGGAGA TTTTCCAATC GGATGATGAA GCTCAATCAG GAGAATGGTG TGGGAGGACC 
CGTGCTATAA ATATTCAATG GCCGACTTTG GTGGATCAGA GAAACAGGAA AAGAGAGCAC 
GTCGCAGTAC TCCATGCAAA GATGTGGTTT GGACGTACAG ATCAGCTCAC AAAATGGAAG 
GAGCACGTAC AACCCGCAGA CATCCGTCGT TTCATGGAAA TGTACGAAGT GCAGACGAAG 
GGACTCGCAT TGAAATGGAA AGATGAAGCT GATATTTACG ATGGGAAATG GGAGAAGACG 
AATGAAGTGC CTCTCGAAAA TGGATGGAAT GCTGTTGGTC ACTGGATTGT GATGAATACT 
CGTCATATGT TTGTACCGAA ACTTGGACAA CACACGGTTC ATGACAAGGC TTTTGAAATC 
CAGAAGAGAA CTGAAGACGG AACGTGGAAA CACTTTAAGT ACACGGATTG CTACGCCGAC 
GAGTTGAAAC CCAAGGATCT TGACAAACAT GCGAAAGGAT GGGAAGTAGG CTCTTGGGCT 
CAAGACAAGT TTCGAAACAA TGGAGACGAA AAAGGATGGG TGTACTCGAC GAATGGTGTC 
TTTTTTGGAA GCGGAGTCCT GACAGACCGA GAAGAAAAAG TTCACCACAA CTTCAGAAAG 
CGTTGTATCA AGAGGCCAAG GAAGCATGAA GGCTATAACA AGGAGTTGGA GGACTTTGAG 
CATTTTCGGA CAACGATGGG AAATGAGAAT TGGGAGTACT CCCCCAGCAA GAAGGAAGGC 
CCATACCACG ATTTGGAAGA CCGAACCGAT CGGATCAGAC GTAGACGATA TGTACGAGAA 
GTTGAGAACA AAGACCCGGA CAGTGAAGAT CCAAGATTTC GCGTTTACGA ATATCAAATG 
CAAACTGGAA AATGGCAACT GAGATGCTAT ATCATGTGGG GAAACGATTT GCTGCCAGTT 
GTCAAGAACT CGTCTCGGGC ATTTGTTCGC ATTTCGTTTG CACAATACTC AAAGCAGACG 
CTTCTCGTGG ACAACTCTCA AAATCCAATT TGGAATGAGA CAGTCATGTT CAAGTCAGTT 
CTGATCGCTG GTGGAACTCG AGATATCATG AAATACCCGC CAGTTGTCTC TGTTGAAGTC 
GTTGGCGAGT GCTCAAACAA TGAGGAGGCA AACCTCGGAC ACTTCGAAAC CAAACCAAAC 
GTCATTTGCG GAAATACGGA TGTTCGTGGC ACGCCACAAT GGTTTCCGTT GAAGTTCTCC 
AATGGACGTA CTCGTGGAGC GGTTCTTGCA TGCTTCGAGC TTTACTCAGA AGAAGATAAA 
GATTTGATTC CACTGGAGCC AAAATGCAAA CACAATTATA AGGAGAGAAG TGAGATCCCA 
ACAGAGTTCA GACCACAGTT CGATAAATTC CACGTGCAAT TTTTATGCTG GGGTGTGAGA 
AATCTCAAGA AGCATAAGCT TCTCGCCGTA CGTCGCCCAT TTGTTGGACT GACAATTGGG 
GATCAAGAGT TCACATTGCA ACCTCTCAAG GATGTCCGAA GAGATCCGAA CTTCCCTGAG 
CCCATGATTG TTTTTGGAGA AGTAATCCTA CCATCTGCTC TTGAGCTATC ACCTCCACTA 
ATCATCAATC TCTTCGATGC TAGGGCCTTC AATCGTCAGC CATTAGTTGG ATCATGCTTG 
GTATCTGATC TCCATAAATA CGTCTCACAT ATTGTTCCCA AGGTCAAAAG TGATCACGCC 
GAAAGATGGG AACAGTTGGA TTTGGTAATT ACGGAGGAGT TTGATCAGAT CATCAGGATG 
GTTCGTGTTC CGACTTTAAC AACTGATCCT ATGGTACCTT TGGATTGGTG GAGCAGATAT 
TATGCATCGA TGTCACAATT TCATAGATCA CCGGGATATC CAGAATCTGG CATGGAGTAT 
GTACGTATCT TCCGCCGACC ACTTGAACAG ATGAACGGAT ACAACAACTT CTCTGATTTC 
CTGGACACAT TCCCGTTTGT CAAATCAATG AAGGGAGATT TCGACGATCC GGAAGAAAAA 
GAAAAGGCTG GAGAATTGAA ATGCCGACTT CTCATCTCGA AACTCAAGAA GGGTAAGCCT 
CCAGCTGCGA TAAATACCGT TGTCGACTTC GTTGGACCAA CTCGATGTCT CGTCCGAGTT 
TACATCATCG AAGCAAATGG GCTGATCTCA AATGCACGGA AAGGACGAGT CGACTCGTAC 
GTTAAACTTC ATTGCGGAAA GCAGAACGTG AACCTGAAGA AGAACTACCG CTCAGAGTGT 
TGTGATCCGA TATTTGGAGA ACGAGTTGAC ATGACGGTTA CGATTCCGCT GGAGAAAGAT 
CTGAAAATCA CTGTGATGGG CAAGCGGAGA ATCCTTTCGG ATCAAGAAAT AGGATCAACT 
ACAATCGATC TGGAAAATCG TCTTCTCACA AAATGGAGAG CAACTGGTGG GCTGAGTGGT 
CAGTTCACTG TACAAGGAGA GCTGCAATGG AGGGATCAGA TGACTCCAAT GGAGATCCTG 
AAGTCGTACT GCTACAAGAT GATGCTATCA GTTCCAAAGA TTGAAAGTAG GCAGACGGAG 
AGAGGAGAAG AGAAGGGAAT TACCATTGAG AAGATCACAT TCTGGTTCAG TGAAGTTCTC 
CATGTCTTTG AGAACGAAGA GATTGCTATG CTGAATTCTC AACGTCAAAA AGCTGGAAAA 
GAAAATTTCA GTGATGGTTC TGATCAACAA AATGAGGACG TATCAGACGG TAGTTGGGAT 
GAAGAGGATC TAGAACGAGA GAAAGAGAAG CTAAAGTGGG AGAAGCATCG GAGCAAGGGC 
AAGCCATTGA AGAAGGTTAC AACGGAAAAG GCAGAAACGG CTGATGGAGA GTTGAGGAAG 
AAGGCGAAGA TGAGAATAAT GGGATCTCAG TTGCAAACAA TTGCTCTCTT CATTCTCCGA 
CAGATGAATT TGGTCCCAGA GCATGTCGAA TCTCGGCCAT TGTTCTCGGA TAAAGGAGGA 
AGAACTCAGA AGGGAGAACT TCGAATGTTC GTTGACATCT TTCCGATGGA ATATGGCGCG 
ATTCCTGCTC CATTCAACAT TGCACCACGG AAACCCATCA ACTATCAGCT ACGTATCGCG 
GTCATGGATG TACGAGGAGC GATTCCAGTC AAAAGAAGCT TTGCCGAGCC TGTCTCCGAT 
CTTTACGTCA AGGCATTCAT CAATGGAATG ACTAAGGGAC ACAAGACGGA TACGCATTTC 
AGAGTTCTCG ATGGAACCGG GGAGTTCAAC TGGCGTTTCC TGCTCAACTT CGACTACAAT 
CCTTGGGAGA AGAAGGTGGT CGCGTACACC AAAAACCGAT TCTTCTGCAA ACCAGTGGAA 
GAACTCGTCG ATCCGATTCT TGTCATTGAG CTTTGGGATA AGAACAAGTT CCGCAAGGAT 
CGTCTTCTCG GTGACATTGA ACTGGATCTT CTCGATTTCA TTGAGGGAAT CGGTTCTCCA 
TCCGACGTTG GTGTCTACTC AACAAAGAAG AGACAGCGTG GAGTGAAGTG TCCAAAGTGT 
TGTACCAGGA GAGGATGTCT CTGCAAATGC TGTATCTTCT GCTTTGAGAC CAAGTGCTTG 
TGTGGAAAAC GCAAAGTGAA GAAGAAGCCA TTCCCGAAGC CTGTGTTGTT CGTGGAACCA 
GAAGGTTACG ATGATACTGT GAACATCTTC GAATCTCGCA ACTTGTACGG TTGGTGGCCA 
ATGCTTACCG AGGAGTATCC ACACGAGGAA CCTCAGAATG CCAAGAAGAA GAATGATGAC 
GTTGGAAAGG ATCCTAAATG GATCATGGGT CTTGTTGAGA TGGACATGCT ACTTCTCACC 
AAACAAGAAG CCGATCAAGA GCCAGCTGGA AAGAAACGAT CCGAGCCGAA CCACAGCCCA 
TTCTTGGAAA AACCTGATCG GAAGTCATGG GCCAACTCGT GGTTGGTGTC CCGAATCAAG 
CCGTGCATCA AGTATTTCTG GCACTACTAT GGTCTTCAAA TTCTGCTCTG GCTCATCATT 
ATTGTTATTC TGATTCTCAC AATTTTCGTG TTGTTGCACA CTTGGCCGAC AATTTTAGCC 
GAAATTATAA AGGCAATTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fer-1 ( NM_001129005.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001122477.1 Caenorhabditis elegans Sperm vesicle fusion protein fer-1 (fer-1), partial mRNA.

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CloneID	OCa350824
Clone ID Related Accession (Same CDS sequence)	NM_001129005.3 , NM_001129005.2
Accession Version	NM_001129005.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Sperm vesicle fusion protein fer-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001129005.1. COMPLETENESS: incomplete on both ends.

ATGACAAGAA CAGTTACTCA TGCAATTCCA AAGTGGCGAC AAAATATTCT CTACACAATA 
AAGAACCAGC CACTGGAGAA GCTTGCTGCC AAAGTTTTGA CTCTTCGGCT CGTGCGTCCT 
ACAGCACTTG GTGAATCAAG CGTCGGAGAG TTCAGCTGCT ACCTCTCGGA AGTAATTCAC 
TCTCCCGACA GATCTGTCAT TGCAAAATGG GTCGCTCTGG GATTTCCTGG AATGGATGAC 
GAGGAACCGG ACGATATCGC CTATGAGAAC TGTGGATTCC TGAAGGTGAC GCTCTCTGTG 
TACCGGATCG ACGAGTCACC ACCAACGCTG ATTGATGACG ATGGGAAGGA GCAAATCTGG 
AGTGGAGCAC ATCTCATTGA TTATACTCTT AAGATCAGAG TGTTCTCCGT GGAACAGCTC 
ATCCGACAGA TGATCAATGA GAGGAAAATC AAGAAGAAGC AAAGATTCTA TGTGATGGTT 
CAGTGTGGAG CACACAAGAC CGAAACAACA ATGGAATCTG CGTTTCTTGA TGAAGATGCA 
AACATCGCAT CAGTGAAGTT TGAGCAAGAG ATCTATCTTC CAATTCAATG GCCAACTGTC 
ATCTCAGAGA TTATTTTCTC ATTGTTCGAC AAAAAGGGAC GAGGCAAAAC GTGCATAGGA 
AAAGCCACTC TTCCAATGAA AATGATTTAC GAGCCAGGAG AGACTGGATA CCTTCCGACT 
TTTGGACCTG CGTTTCTCAA TGTATTCGAT TGCGAACGCG TGACTCGATT CAGCATCTTC 
TCAAGAAAAA TGCGAGGATC CCAGCAGGAT GGGAGCAGAT TTGTTGGGCG TTTGTTCATT 
GCGATCGATT GTGTAGAGTA TATGGAGAAG GCTAATGCTC AGATCATGCA TCTTGATCAT 
TCCCCAATTA TGGAAGCCGA ATCGATTTGT AAAAATCTCG AATGGTACAA CTGCTTCTGT 
TCCATGAGTG GCCTCAACAT GATCAATCCT CAATTCGCAT CCGATCCAGT CTGCATAATG 
ATGTCAATCG GTTCATTTGG AAGCACGAGC AACGAGTTCA GATCATGCAA TAGCTCAACA 
CTGCCAGCAG AGCCAAACTG GGATGGATGC AAGTACTTCA CGATGCCATG GGGAAATCTC 
CGACCAGTTG CCGAAGTCAA TGCTCCGTGG GAAGTTATTG AGCATCGGAT TGAAATGAGC 
AATGTTCTCA TGAAAATGAC GAATATGCTT GATACGATGA TTTGGGAGGT TCGACGCATT 
GGAAACAAAG CAATCGACCA TGTTTCGTCA GTTGGAATGG AAGCTTTGGA GTGCTTGGAG 
CAGCAAATTG ATTCGGCTAG CAAGTATTTG GGACGCATCA ACCCCGTTAG CAGCAACGCA 
CTGGATCGCC ATATCCTTGA CTACCGGAAG GAAAAGATCT TGAAGCTGCG AGAGCATTTT 
GAGAAGGAAG CCTTCAGTAT AGATTATTCT GATGGAGAAG TCGATGCGAA GCTGCTCCGA 
ATGCTTCTAA AAATTCGATC GATGACTCTT GAGTTGGCAG AAGATGTTCA AATGACCATT 
CCACCCGTTC TCATCAAAAT GATGTCCCAT GGGAAGTTGA TCGGTTTCGC CAAAATCCCG 
ATTTCGGAGA TTTTCCAATC GGATGATGAA GCTCAATCAG GAGAATGGTG TGGGAGGACC 
CGTGCTATAA ATATTCAATG GCCGACTTTG GTGGATCAGA GAAACAGGAA AAGAGAGCAC 
GTCGCAGTAC TCCATGCAAA GATGTGGTTT GGACGTACAG ATCAGCTCAC AAAATGGAAG 
GAGCACGTAC AACCCGCAGA CATCCGTCGT TTCATGGAAA TGTACGAAGT GCAGACGAAG 
GGACTCGCAT TGAAATGGAA AGATGAAGCT GATATTTACG ATGGGAAATG GGAGAAGACG 
AATGAAGTGC CTCTCGAAAA TGGATGGAAT GCTGTTGGTC ACTGGATTGT GATGAATACT 
CGTCATATGT TTGTACCGAA ACTTGGACAA CACACGGTTC ATGACAAGGC TTTTGAAATC 
CAGAAGAGAA CTGAAGACGG AACGTGGAAA CACTTTAAGT ACACGGATTG CTACGCCGAC 
GAGTTGAAAC CCAAGGATCT TGACAAACAT GCGAAAGGAT GGGAAGTAGG CTCTTGGGCT 
CAAGACAAGT TTCGAAACAA TGGAGACGAA AAAGGATGGG TGTACTCGAC GAATGGTGTC 
TTTTTTGGAA GCGGAGTCCT GACAGACCGA GAAGAAAAAG TTCACCACAA CTTCAGAAAG 
CGTTGTATCA AGAGGCCAAG GAAGCATGAA GGCTATAACA AGGAGTTGGA GGACTTTGAG 
CATTTTCGGA CAACGATGGG AAATGAGAAT TGGGAGTACT CCCCCAGCAA GAAGGAAGGC 
CCATACCACG ATTTGGAAGA CCGAACCGAT CGGATCAGAC GTAGACGATA TGTACGAGAA 
GTTGAGAACA AAGACCCGGA CAGTGAAGAT CCAAGATTTC GCGTTTACGA ATATCAAATG 
CAAACTGGAA AATGGCAACT GAGATGCTAT ATCATGTGGG GAAACGATTT GCTGCCAGTT 
GTCAAGAACT CGTCTCGGGC ATTTGTTCGC ATTTCGTTTG CACAATACTC AAAGCAGACG 
CTTCTCGTGG ACAACTCTCA AAATCCAATT TGGAATGAGA CAGTCATGTT CAAGTCAGTT 
CTGATCGCTG GTGGAACTCG AGATATCATG AAATACCCGC CAGTTGTCTC TGTTGAAGTC 
GTTGGCGAGT GCTCAAACAA TGAGGAGGCA AACCTCGGAC ACTTCGAAAC CAAACCAAAC 
GTCATTTGCG GAAATACGGA TGTTCGTGGC ACGCCACAAT GGTTTCCGTT GAAGTTCTCC 
AATGGACGTA CTCGTGGAGC GGTTCTTGCA TGCTTCGAGC TTTACTCAGA AGAAGATAAA 
GATTTGATTC CACTGGAGCC AAAATGCAAA CACAATTATA AGGAGAGAAG TGAGATCCCA 
ACAGAGTTCA GACCACAGTT CGATAAATTC CACGTGCAAT TTTTATGCTG GGGTGTGAGA 
AATCTCAAGA AGCATAAGCT TCTCGCCGTA CGTCGCCCAT TTGTTGGACT GACAATTGGG 
GATCAAGAGT TCACATTGCA ACCTCTCAAG GATGTCCGAA GAGATCCGAA CTTCCCTGAG 
CCCATGATTG TTTTTGGAGA AGTAATCCTA CCATCTGCTC TTGAGCTATC ACCTCCACTA 
ATCATCAATC TCTTCGATGC TAGGGCCTTC AATCGTCAGC CATTAGTTGG ATCATGCTTG 
GTATCTGATC TCCATAAATA CGTCTCACAT ATTGTTCCCA AGGTCAAAAG TGATCACGCC 
GAAAGATGGG AACAGTTGGA TTTGGTAATT ACGGAGGAGT TTGATCAGAT CATCAGGATG 
GTTCGTGTTC CGACTTTAAC AACTGATCCT ATGGTACCTT TGGATTGGTG GAGCAGATAT 
TATGCATCGA TGTCACAATT TCATAGATCA CCGGGATATC CAGAATCTGG CATGGAGTAT 
GTACGTATCT TCCGCCGACC ACTTGAACAG ATGAACGGAT ACAACAACTT CTCTGATTTC 
CTGGACACAT TCCCGTTTGT CAAATCAATG AAGGGAGATT TCGACGATCC GGAAGAAAAA 
GAAAAGGCTG GAGAATTGAA ATGCCGACTT CTCATCTCGA AACTCAAGAA GGGTAAGCCT 
CCAGCTGCGA TAAATACCGT TGTCGACTTC GTTGGACCAA CTCGATGTCT CGTCCGAGTT 
TACATCATCG AAGCAAATGG GCTGATCTCA AATGCACGGA AAGGACGAGT CGACTCGTAC 
GTTAAACTTC ATTGCGGAAA GCAGAACGTG AACCTGAAGA AGAACTACCG CTCAGAGTGT 
TGTGATCCGA TATTTGGAGA ACGAGTTGAC ATGACGGTTA CGATTCCGCT GGAGAAAGAT 
CTGAAAATCA CTGTGATGGG CAAGCGGAGA ATCCTTTCGG ATCAAGAAAT AGGATCAACT 
ACAATCGATC TGGAAAATCG TCTTCTCACA AAATGGAGAG CAACTGGTGG GCTGAGTGGT 
CAGTTCACTG TACAAGGAGA GCTGCAATGG AGGGATCAGA TGACTCCAAT GGAGATCCTG 
AAGTCGTACT GCTACAAGAT GATGCTATCA GTTCCAAAGA TTGAAAGTAG GCAGACGGAG 
AGAGGAGAAG AGAAGGGAAT TACCATTGAG AAGATCACAT TCTGGTTCAG TGAAGTTCTC 
CATGTCTTTG AGAACGAAGA GATTGCTATG CTGAATTCTC AACGTCAAAA AGCTGGAAAA 
GAAAATTTCA GTGATGGTTC TGATCAACAA AATGAGGACG TATCAGACGG TAGTTGGGAT 
GAAGAGGATC TAGAACGAGA GAAAGAGAAG CTAAAGTGGG AGAAGCATCG GAGCAAGGGC 
AAGCCATTGA AGAAGGTTAC AACGGAAAAG GCAGAAACGG CTGATGGAGA GTTGAGGAAG 
AAGGCGAAGA TGAGAATAAT GGGATCTCAG TTGCAAACAA TTGCTCTCTT CATTCTCCGA 
CAGATGAATT TGGTCCCAGA GCATGTCGAA TCTCGGCCAT TGTTCTCGGA TAAAGGAGGA 
AGAACTCAGA AGGGAGAACT TCGAATGTTC GTTGACATCT TTCCGATGGA ATATGGCGCG 
ATTCCTGCTC CATTCAACAT TGCACCACGG AAACCCATCA ACTATCAGCT ACGTATCGCG 
GTCATGGATG TACGAGGAGC GATTCCAGTC AAAAGAAGCT TTGCCGAGCC TGTCTCCGAT 
CTTTACGTCA AGGCATTCAT CAATGGAATG ACTAAGGGAC ACAAGACGGA TACGCATTTC 
AGAGTTCTCG ATGGAACCGG GGAGTTCAAC TGGCGTTTCC TGCTCAACTT CGACTACAAT 
CCTTGGGAGA AGAAGGTGGT CGCGTACACC AAAAACCGAT TCTTCTGCAA ACCAGTGGAA 
GAACTCGTCG ATCCGATTCT TGTCATTGAG CTTTGGGATA AGAACAAGTT CCGCAAGGAT 
CGTCTTCTCG GTGACATTGA ACTGGATCTT CTCGATTTCA TTGAGGGAAT CGGTTCTCCA 
TCCGACGTTG GTGTCTACTC AACAAAGAAG AGACAGCGTG GAGTGAAGTG TCCAAAGTGT 
TGTACCAGGA GAGGATGTCT CTGCAAATGC TGTATCTTCT GCTTTGAGAC CAAGTGCTTG 
TGTGGAAAAC GCAAAGTGAA GAAGAAGCCA TTCCCGAAGC CTGTGTTGTT CGTGGAACCA 
GAAGGTTACG ATGATACTGT GAACATCTTC GAATCTCGCA ACTTGTACGG TTGGTGGCCA 
ATGCTTACCG AGGAGTATCC ACACGAGGAA CCTCAGAATG CCAAGAAGAA GAATGATGAC 
GTTGGAAAGG ATCCTAAATG GATCATGGGT CTTGTTGAGA TGGACATGCT ACTTCTCACC 
AAACAAGAAG CCGATCAAGA GCCAGCTGGA AAGAAACGAT CCGAGCCGAA CCACAGCCCA 
TTCTTGGAAA AACCTGATCG GAAGTCATGG GCCAACTCGT GGTTGGTGTC CCGAATCAAG 
CCGTGCATCA AGTATTTCTG GCACTACTAT GGTCTTCAAA TTCTGCTCTG GCTCATCATT 
ATTGTTATTC TGATTCTCAC AATTTTCGTG TTGTTGCACA CTTGGCCGAC AATTTTAGCC 
GAAATTATAA AGGCAATTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122477.1
CDS	1..5724
Translation	MTRTVTHAIPKWRQNILYTIKNQPLEKLAAKVLTLRLVRPTALGESSVGEFSCYLSEVIHSPDRSVIAKWVALGFPGMDDEEPDDIAYENCGFLKVTLSVYRIDESPPTLIDDDGKEQIWSGAHLIDYTLKIRVFSVEQLIRQMINERKIKKKQRFYVMVQCGAHKTETTMESAFLDEDANIASVKFEQEIYLPIQWPTVISEIIFSLFDKKGRGKTCIGKATLPMKMIYEPGETGYLPTFGPAFLNVFDCERVTRFSIFSRKMRGSQQDGSRFVGRLFIAIDCVEYMEKANAQIMHLDHSPIMEAESICKNLEWYNCFCSMSGLNMINPQFASDPVCIMMSIGSFGSTSNEFRSCNSSTLPAEPNWDGCKYFTMPWGNLRPVAEVNAPWEVIEHRIEMSNVLMKMTNMLDTMIWEVRRIGNKAIDHVSSVGMEALECLEQQIDSASKYLGRINPVSSNALDRHILDYRKEKILKLREHFEKEAFSIDYSDGEVDAKLLRMLLKIRSMTLELAEDVQMTIPPVLIKMMSHGKLIGFAKIPISEIFQSDDEAQSGEWCGRTRAINIQWPTLVDQRNRKREHVAVLHAKMWFGRTDQLTKWKEHVQPADIRRFMEMYEVQTKGLALKWKDEADIYDGKWEKTNEVPLENGWNAVGHWIVMNTRHMFVPKLGQHTVHDKAFEIQKRTEDGTWKHFKYTDCYADELKPKDLDKHAKGWEVGSWAQDKFRNNGDEKGWVYSTNGVFFGSGVLTDREEKVHHNFRKRCIKRPRKHEGYNKELEDFEHFRTTMGNENWEYSPSKKEGPYHDLEDRTDRIRRRRYVREVENKDPDSEDPRFRVYEYQMQTGKWQLRCYIMWGNDLLPVVKNSSRAFVRISFAQYSKQTLLVDNSQNPIWNETVMFKSVLIAGGTRDIMKYPPVVSVEVVGECSNNEEANLGHFETKPNVICGNTDVRGTPQWFPLKFSNGRTRGAVLACFELYSEEDKDLIPLEPKCKHNYKERSEIPTEFRPQFDKFHVQFLCWGVRNLKKHKLLAVRRPFVGLTIGDQEFTLQPLKDVRRDPNFPEPMIVFGEVILPSALELSPPLIINLFDARAFNRQPLVGSCLVSDLHKYVSHIVPKVKSDHAERWEQLDLVITEEFDQIIRMVRVPTLTTDPMVPLDWWSRYYASMSQFHRSPGYPESGMEYVRIFRRPLEQMNGYNNFSDFLDTFPFVKSMKGDFDDPEEKEKAGELKCRLLISKLKKGKPPAAINTVVDFVGPTRCLVRVYIIEANGLISNARKGRVDSYVKLHCGKQNVNLKKNYRSECCDPIFGERVDMTVTIPLEKDLKITVMGKRRILSDQEIGSTTIDLENRLLTKWRATGGLSGQFTVQGELQWRDQMTPMEILKSYCYKMMLSVPKIESRQTERGEEKGITIEKITFWFSEVLHVFENEEIAMLNSQRQKAGKENFSDGSDQQNEDVSDGSWDEEDLEREKEKLKWEKHRSKGKPLKKVTTEKAETADGELRKKAKMRIMGSQLQTIALFILRQMNLVPEHVESRPLFSDKGGRTQKGELRMFVDIFPMEYGAIPAPFNIAPRKPINYQLRIAVMDVRGAIPVKRSFAEPVSDLYVKAFINGMTKGHKTDTHFRVLDGTGEFNWRFLLNFDYNPWEKKVVAYTKNRFFCKPVEELVDPILVIELWDKNKFRKDRLLGDIELDLLDFIEGIGSPSDVGVYSTKKRQRGVKCPKCCTRRGCLCKCCIFCFETKCLCGKRKVKKKPFPKPVLFVEPEGYDDTVNIFESRNLYGWWPMLTEEYPHEEPQNAKKKNDDVGKDPKWIMGLVEMDMLLLTKQEADQEPAGKKRSEPNHSPFLEKPDRKSWANSWLVSRIKPCIKYFWHYYGLQILLWLIIIVILILTIFVLLHTWPTILAEIIKAIF

Target ORF information:

RefSeq Version	NM_001129005.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Sperm vesicle fusion protein fer-1 (fer-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129005.2

ORF Insert Sequence:

ATGACAAGAA CAGTTACTCA TGCAATTCCA AAGTGGCGAC AAAATATTCT CTACACAATA 
AAGAACCAGC CACTGGAGAA GCTTGCTGCC AAAGTTTTGA CTCTTCGGCT CGTGCGTCCT 
ACAGCACTTG GTGAATCAAG CGTCGGAGAG TTCAGCTGCT ACCTCTCGGA AGTAATTCAC 
TCTCCCGACA GATCTGTCAT TGCAAAATGG GTCGCTCTGG GATTTCCTGG AATGGATGAC 
GAGGAACCGG ACGATATCGC CTATGAGAAC TGTGGATTCC TGAAGGTGAC GCTCTCTGTG 
TACCGGATCG ACGAGTCACC ACCAACGCTG ATTGATGACG ATGGGAAGGA GCAAATCTGG 
AGTGGAGCAC ATCTCATTGA TTATACTCTT AAGATCAGAG TGTTCTCCGT GGAACAGCTC 
ATCCGACAGA TGATCAATGA GAGGAAAATC AAGAAGAAGC AAAGATTCTA TGTGATGGTT 
CAGTGTGGAG CACACAAGAC CGAAACAACA ATGGAATCTG CGTTTCTTGA TGAAGATGCA 
AACATCGCAT CAGTGAAGTT TGAGCAAGAG ATCTATCTTC CAATTCAATG GCCAACTGTC 
ATCTCAGAGA TTATTTTCTC ATTGTTCGAC AAAAAGGGAC GAGGCAAAAC GTGCATAGGA 
AAAGCCACTC TTCCAATGAA AATGATTTAC GAGCCAGGAG AGACTGGATA CCTTCCGACT 
TTTGGACCTG CGTTTCTCAA TGTATTCGAT TGCGAACGCG TGACTCGATT CAGCATCTTC 
TCAAGAAAAA TGCGAGGATC CCAGCAGGAT GGGAGCAGAT TTGTTGGGCG TTTGTTCATT 
GCGATCGATT GTGTAGAGTA TATGGAGAAG GCTAATGCTC AGATCATGCA TCTTGATCAT 
TCCCCAATTA TGGAAGCCGA ATCGATTTGT AAAAATCTCG AATGGTACAA CTGCTTCTGT 
TCCATGAGTG GCCTCAACAT GATCAATCCT CAATTCGCAT CCGATCCAGT CTGCATAATG 
ATGTCAATCG GTTCATTTGG AAGCACGAGC AACGAGTTCA GATCATGCAA TAGCTCAACA 
CTGCCAGCAG AGCCAAACTG GGATGGATGC AAGTACTTCA CGATGCCATG GGGAAATCTC 
CGACCAGTTG CCGAAGTCAA TGCTCCGTGG GAAGTTATTG AGCATCGGAT TGAAATGAGC 
AATGTTCTCA TGAAAATGAC GAATATGCTT GATACGATGA TTTGGGAGGT TCGACGCATT 
GGAAACAAAG CAATCGACCA TGTTTCGTCA GTTGGAATGG AAGCTTTGGA GTGCTTGGAG 
CAGCAAATTG ATTCGGCTAG CAAGTATTTG GGACGCATCA ACCCCGTTAG CAGCAACGCA 
CTGGATCGCC ATATCCTTGA CTACCGGAAG GAAAAGATCT TGAAGCTGCG AGAGCATTTT 
GAGAAGGAAG CCTTCAGTAT AGATTATTCT GATGGAGAAG TCGATGCGAA GCTGCTCCGA 
ATGCTTCTAA AAATTCGATC GATGACTCTT GAGTTGGCAG AAGATGTTCA AATGACCATT 
CCACCCGTTC TCATCAAAAT GATGTCCCAT GGGAAGTTGA TCGGTTTCGC CAAAATCCCG 
ATTTCGGAGA TTTTCCAATC GGATGATGAA GCTCAATCAG GAGAATGGTG TGGGAGGACC 
CGTGCTATAA ATATTCAATG GCCGACTTTG GTGGATCAGA GAAACAGGAA AAGAGAGCAC 
GTCGCAGTAC TCCATGCAAA GATGTGGTTT GGACGTACAG ATCAGCTCAC AAAATGGAAG 
GAGCACGTAC AACCCGCAGA CATCCGTCGT TTCATGGAAA TGTACGAAGT GCAGACGAAG 
GGACTCGCAT TGAAATGGAA AGATGAAGCT GATATTTACG ATGGGAAATG GGAGAAGACG 
AATGAAGTGC CTCTCGAAAA TGGATGGAAT GCTGTTGGTC ACTGGATTGT GATGAATACT 
CGTCATATGT TTGTACCGAA ACTTGGACAA CACACGGTTC ATGACAAGGC TTTTGAAATC 
CAGAAGAGAA CTGAAGACGG AACGTGGAAA CACTTTAAGT ACACGGATTG CTACGCCGAC 
GAGTTGAAAC CCAAGGATCT TGACAAACAT GCGAAAGGAT GGGAAGTAGG CTCTTGGGCT 
CAAGACAAGT TTCGAAACAA TGGAGACGAA AAAGGATGGG TGTACTCGAC GAATGGTGTC 
TTTTTTGGAA GCGGAGTCCT GACAGACCGA GAAGAAAAAG TTCACCACAA CTTCAGAAAG 
CGTTGTATCA AGAGGCCAAG GAAGCATGAA GGCTATAACA AGGAGTTGGA GGACTTTGAG 
CATTTTCGGA CAACGATGGG AAATGAGAAT TGGGAGTACT CCCCCAGCAA GAAGGAAGGC 
CCATACCACG ATTTGGAAGA CCGAACCGAT CGGATCAGAC GTAGACGATA TGTACGAGAA 
GTTGAGAACA AAGACCCGGA CAGTGAAGAT CCAAGATTTC GCGTTTACGA ATATCAAATG 
CAAACTGGAA AATGGCAACT GAGATGCTAT ATCATGTGGG GAAACGATTT GCTGCCAGTT 
GTCAAGAACT CGTCTCGGGC ATTTGTTCGC ATTTCGTTTG CACAATACTC AAAGCAGACG 
CTTCTCGTGG ACAACTCTCA AAATCCAATT TGGAATGAGA CAGTCATGTT CAAGTCAGTT 
CTGATCGCTG GTGGAACTCG AGATATCATG AAATACCCGC CAGTTGTCTC TGTTGAAGTC 
GTTGGCGAGT GCTCAAACAA TGAGGAGGCA AACCTCGGAC ACTTCGAAAC CAAACCAAAC 
GTCATTTGCG GAAATACGGA TGTTCGTGGC ACGCCACAAT GGTTTCCGTT GAAGTTCTCC 
AATGGACGTA CTCGTGGAGC GGTTCTTGCA TGCTTCGAGC TTTACTCAGA AGAAGATAAA 
GATTTGATTC CACTGGAGCC AAAATGCAAA CACAATTATA AGGAGAGAAG TGAGATCCCA 
ACAGAGTTCA GACCACAGTT CGATAAATTC CACGTGCAAT TTTTATGCTG GGGTGTGAGA 
AATCTCAAGA AGCATAAGCT TCTCGCCGTA CGTCGCCCAT TTGTTGGACT GACAATTGGG 
GATCAAGAGT TCACATTGCA ACCTCTCAAG GATGTCCGAA GAGATCCGAA CTTCCCTGAG 
CCCATGATTG TTTTTGGAGA AGTAATCCTA CCATCTGCTC TTGAGCTATC ACCTCCACTA 
ATCATCAATC TCTTCGATGC TAGGGCCTTC AATCGTCAGC CATTAGTTGG ATCATGCTTG 
GTATCTGATC TCCATAAATA CGTCTCACAT ATTGTTCCCA AGGTCAAAAG TGATCACGCC 
GAAAGATGGG AACAGTTGGA TTTGGTAATT ACGGAGGAGT TTGATCAGAT CATCAGGATG 
GTTCGTGTTC CGACTTTAAC AACTGATCCT ATGGTACCTT TGGATTGGTG GAGCAGATAT 
TATGCATCGA TGTCACAATT TCATAGATCA CCGGGATATC CAGAATCTGG CATGGAGTAT 
GTACGTATCT TCCGCCGACC ACTTGAACAG ATGAACGGAT ACAACAACTT CTCTGATTTC 
CTGGACACAT TCCCGTTTGT CAAATCAATG AAGGGAGATT TCGACGATCC GGAAGAAAAA 
GAAAAGGCTG GAGAATTGAA ATGCCGACTT CTCATCTCGA AACTCAAGAA GGGTAAGCCT 
CCAGCTGCGA TAAATACCGT TGTCGACTTC GTTGGACCAA CTCGATGTCT CGTCCGAGTT 
TACATCATCG AAGCAAATGG GCTGATCTCA AATGCACGGA AAGGACGAGT CGACTCGTAC 
GTTAAACTTC ATTGCGGAAA GCAGAACGTG AACCTGAAGA AGAACTACCG CTCAGAGTGT 
TGTGATCCGA TATTTGGAGA ACGAGTTGAC ATGACGGTTA CGATTCCGCT GGAGAAAGAT 
CTGAAAATCA CTGTGATGGG CAAGCGGAGA ATCCTTTCGG ATCAAGAAAT AGGATCAACT 
ACAATCGATC TGGAAAATCG TCTTCTCACA AAATGGAGAG CAACTGGTGG GCTGAGTGGT 
CAGTTCACTG TACAAGGAGA GCTGCAATGG AGGGATCAGA TGACTCCAAT GGAGATCCTG 
AAGTCGTACT GCTACAAGAT GATGCTATCA GTTCCAAAGA TTGAAAGTAG GCAGACGGAG 
AGAGGAGAAG AGAAGGGAAT TACCATTGAG AAGATCACAT TCTGGTTCAG TGAAGTTCTC 
CATGTCTTTG AGAACGAAGA GATTGCTATG CTGAATTCTC AACGTCAAAA AGCTGGAAAA 
GAAAATTTCA GTGATGGTTC TGATCAACAA AATGAGGACG TATCAGACGG TAGTTGGGAT 
GAAGAGGATC TAGAACGAGA GAAAGAGAAG CTAAAGTGGG AGAAGCATCG GAGCAAGGGC 
AAGCCATTGA AGAAGGTTAC AACGGAAAAG GCAGAAACGG CTGATGGAGA GTTGAGGAAG 
AAGGCGAAGA TGAGAATAAT GGGATCTCAG TTGCAAACAA TTGCTCTCTT CATTCTCCGA 
CAGATGAATT TGGTCCCAGA GCATGTCGAA TCTCGGCCAT TGTTCTCGGA TAAAGGAGGA 
AGAACTCAGA AGGGAGAACT TCGAATGTTC GTTGACATCT TTCCGATGGA ATATGGCGCG 
ATTCCTGCTC CATTCAACAT TGCACCACGG AAACCCATCA ACTATCAGCT ACGTATCGCG 
GTCATGGATG TACGAGGAGC GATTCCAGTC AAAAGAAGCT TTGCCGAGCC TGTCTCCGAT 
CTTTACGTCA AGGCATTCAT CAATGGAATG ACTAAGGGAC ACAAGACGGA TACGCATTTC 
AGAGTTCTCG ATGGAACCGG GGAGTTCAAC TGGCGTTTCC TGCTCAACTT CGACTACAAT 
CCTTGGGAGA AGAAGGTGGT CGCGTACACC AAAAACCGAT TCTTCTGCAA ACCAGTGGAA 
GAACTCGTCG ATCCGATTCT TGTCATTGAG CTTTGGGATA AGAACAAGTT CCGCAAGGAT 
CGTCTTCTCG GTGACATTGA ACTGGATCTT CTCGATTTCA TTGAGGGAAT CGGTTCTCCA 
TCCGACGTTG GTGTCTACTC AACAAAGAAG AGACAGCGTG GAGTGAAGTG TCCAAAGTGT 
TGTACCAGGA GAGGATGTCT CTGCAAATGC TGTATCTTCT GCTTTGAGAC CAAGTGCTTG 
TGTGGAAAAC GCAAAGTGAA GAAGAAGCCA TTCCCGAAGC CTGTGTTGTT CGTGGAACCA 
GAAGGTTACG ATGATACTGT GAACATCTTC GAATCTCGCA ACTTGTACGG TTGGTGGCCA 
ATGCTTACCG AGGAGTATCC ACACGAGGAA CCTCAGAATG CCAAGAAGAA GAATGATGAC 
GTTGGAAAGG ATCCTAAATG GATCATGGGT CTTGTTGAGA TGGACATGCT ACTTCTCACC 
AAACAAGAAG CCGATCAAGA GCCAGCTGGA AAGAAACGAT CCGAGCCGAA CCACAGCCCA 
TTCTTGGAAA AACCTGATCG GAAGTCATGG GCCAACTCGT GGTTGGTGTC CCGAATCAAG 
CCGTGCATCA AGTATTTCTG GCACTACTAT GGTCTTCAAA TTCTGCTCTG GCTCATCATT 
ATTGTTATTC TGATTCTCAC AATTTTCGTG TTGTTGCACA CTTGGCCGAC AATTTTAGCC 
GAAATTATAA AGGCAATTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fer-1 ( NM_059936.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492337.1 Caenorhabditis elegans Sperm vesicle fusion protein fer-1 (fer-1), partial mRNA.

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CloneID	OCa335673
Clone ID Related Accession (Same CDS sequence)	NM_059936.3 , NM_059936.4
Accession Version	NM_059936.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Sperm vesicle fusion protein fer-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 27, 2008 this sequence version replaced NM_059936.2. COMPLETENESS: incomplete on both ends.

ATGACGGTCA AGGAAAAGCT ACTGAAAGTG AAGCAAAAGT TTAAGCGAGG GGATTCATCC 
AGTGATGTGG ATTCCGGCCC GGAGAGAGAT CGGGATGAAG ACGATTCGGA TAACGAATCG 
AGGAATACAA GCAAGACGAG CAAATCCAGC AAAAAGAGCA AATCGGGAAA GAGCACGGGA 
ACATCGGAGA TGACTGAGCT ATCGGACGAC GGTGGATCTG ACATCGAGCT GCTGCCAGAT 
GATGGAAAAA GCTTTGGAAA ACGGAAACTG CAGGACACTG ACAAGCCATG TACCTGGAAT 
GTTCTTGTAC GAATTATTGA GGCAAAGGAT GTCAGCACAG GATCCGCAAG AGTTCGAACG 
ATCTTTGACG GAAATTCAAA AATGACAAGA ACAGTTACTC ATGCAATTCC AAAGTGGCGA 
CAAAATATTC TCTACACAAT AAAGAACCAG CCACTGGAGA AGCTTGCTGC CAAAGTTTTG 
ACTCTTCGGC TCGTGCGTCC TACAGCACTT GGTGAATCAA GCGTCGGAGA GTTCAGCTGC 
TACCTCTCGG AAGTAATTCA CTCTCCCGAC AGATCTGTCA TTGCAAAATG GGTCGCTCTG 
GGATTTCCTG GAATGGATGA CGAGGAACCG GACGATATCG CCTATGAGAA CTGTGGATTC 
CTGAAGGTGA CGCTCTCTGT GTACCGGATC GACGAGTCAC CACCAACGCT GATTGATGAC 
GATGGGAAGG AGCAAATCTG GAGTGGAGCA CATCTCATTG ATTATACTCT TAAGATCAGA 
GTGTTCTCCG TGGAACAGCT CATCCGACAG ATGATCAATG AGAGGAAAAT CAAGAAGAAG 
CAAAGATTCT ATGTGATGGT TCAGTGTGGA GCACACAAGA CCGAAACAAC AATGGAATCT 
GCGTTTCTTG ATGAAGATGC AAACATCGCA TCAGTGAAGT TTGAGCAAGA GATCTATCTT 
CCAATTCAAT GGCCAACTGT CATCTCAGAG ATTATTTTCT CATTGTTCGA CAAAAAGGGA 
CGAGGCAAAA CGTGCATAGG AAAAGCCACT CTTCCAATGA AAATGATTTA CGAGCCAGGA 
GAGACTGGAT ACCTTCCGAC TTTTGGACCT GCGTTTCTCA ATGTATTCGA TTGCGAACGC 
GTGACTCGAT TCAGCATCTT CTCAAGAAAA ATGCGAGGAT CCCAGCAGGA TGGGAGCAGA 
TTTGTTGGGC GTTTGTTCAT TGCGATCGAT TGTGTAGAGT ATATGGAGAA GGCTAATGCT 
CAGATCATGC ATCTTGATCA TTCCCCAATT ATGGAAGCCG AATCGATTTG TAAAAATCTC 
GAATGGTACA ACTGCTTCTG TTCCATGAGT GGCCTCAACA TGATCAATCC TCAATTCGCA 
TCCGATCCAG TCTGCATAAT GATGTCAATC GGTTCATTTG GAAGCACGAG CAACGAGTTC 
AGATCATGCA ATAGCTCAAC ACTGCCAGCA GAGCCAAACT GGGATGGATG CAAGTACTTC 
ACGATGCCAT GGGGAAATCT CCGACCAGTT GCCGAAGTCA ATGCTCCGTG GGAAGTTATT 
GAGCATCGGA TTGAAATGAG CAATGTTCTC ATGAAAATGA CGAATATGCT TGATACGATG 
ATTTGGGAGG TTCGACGCAT TGGAAACAAA GCAATCGACC ATGTTTCGTC AGTTGGAATG 
GAAGCTTTGG AGTGCTTGGA GCAGCAAATT GATTCGGCTA GCAAGTATTT GGGACGCATC 
AACCCCGTTA GCAGCAACGC ACTGGATCGC CATATCCTTG ACTACCGGAA GGAAAAGATC 
TTGAAGCTGC GAGAGCATTT TGAGAAGGAA GCCTTCAGTA TAGATTATTC TGATGGAGAA 
GTCGATGCGA AGCTGCTCCG AATGCTTCTA AAAATTCGAT CGATGACTCT TGAGTTGGCA 
GAAGATGTTC AAATGACCAT TCCACCCGTT CTCATCAAAA TGATGTCCCA TGGGAAGTTG 
ATCGGTTTCG CCAAAATCCC GATTTCGGAG ATTTTCCAAT CGGATGATGA AGCTCAATCA 
GGAGAATGGT GTGGGAGGAC CCGTGCTATA AATATTCAAT GGCCGACTTT GGTGGATCAG 
AGAAACAGGA AAAGAGAGCA CGTCGCAGTA CTCCATGCAA AGATGTGGTT TGGACGTACA 
GATCAGCTCA CAAAATGGAA GGAGCACGTA CAACCCGCAG ACATCCGTCG TTTCATGGAA 
ATGTACGAAG TGCAGACGAA GGGACTCGCA TTGAAATGGA AAGATGAAGC TGATATTTAC 
GATGGGAAAT GGGAGAAGAC GAATGAAGTG CCTCTCGAAA ATGGATGGAA TGCTGTTGGT 
CACTGGATTG TGATGAATAC TCGTCATATG TTTGTACCGA AACTTGGACA ACACACGGTT 
CATGACAAGG CTTTTGAAAT CCAGAAGAGA ACTGAAGACG GAACGTGGAA ACACTTTAAG 
TACACGGATT GCTACGCCGA CGAGTTGAAA CCCAAGGATC TTGACAAACA TGCGAAAGGA 
TGGGAAGTAG GCTCTTGGGC TCAAGACAAG TTTCGAAACA ATGGAGACGA AAAAGGATGG 
GTGTACTCGA CGAATGGTGT CTTTTTTGGA AGCGGAGTCC TGACAGACCG AGAAGAAAAA 
GTTCACCACA ACTTCAGAAA GCGTTGTATC AAGAGGCCAA GGAAGCATGA AGGCTATAAC 
AAGGAGTTGG AGGACTTTGA GCATTTTCGG ACAACGATGG GAAATGAGAA TTGGGAGTAC 
TCCCCCAGCA AGAAGGAAGG CCCATACCAC GATTTGGAAG ACCGAACCGA TCGGATCAGA 
CGTAGACGAT ATGTACGAGA AGTTGAGAAC AAAGACCCGG ACAGTGAAGA TCCAAGATTT 
CGCGTTTACG AATATCAAAT GCAAACTGGA AAATGGCAAC TGAGATGCTA TATCATGTGG 
GGAAACGATT TGCTGCCAGT TGTCAAGAAC TCGTCTCGGG CATTTGTTCG CATTTCGTTT 
GCACAATACT CAAAGCAGAC GCTTCTCGTG GACAACTCTC AAAATCCAAT TTGGAATGAG 
ACAGTCATGT TCAAGTCAGT TCTGATCGCT GGTGGAACTC GAGATATCAT GAAATACCCG 
CCAGTTGTCT CTGTTGAAGT CGTTGGCGAG TGCTCAAACA ATGAGGAGGC AAACCTCGGA 
CACTTCGAAA CCAAACCAAA CGTCATTTGC GGAAATACGG ATGTTCGTGG CACGCCACAA 
TGGTTTCCGT TGAAGTTCTC CAATGGACGT ACTCGTGGAG CGGTTCTTGC ATGCTTCGAG 
CTTTACTCAG AAGAAGATAA AGATTTGATT CCACTGGAGC CAAAATGCAA ACACAATTAT 
AAGGAGAGAA GTGAGATCCC AACAGAGTTC AGACCACAGT TCGATAAATT CCACGTGCAA 
TTTTTATGCT GGGGTGTGAG AAATCTCAAG AAGCATAAGC TTCTCGCCGT ACGTCGCCCA 
TTTGTTGGAC TGACAATTGG GGATCAAGAG TTCACATTGC AACCTCTCAA GGATGTCCGA 
AGAGATCCGA ACTTCCCTGA GCCCATGATT GTTTTTGGAG AAGTAATCCT ACCATCTGCT 
CTTGAGCTAT CACCTCCACT AATCATCAAT CTCTTCGATG CTAGGGCCTT CAATCGTCAG 
CCATTAGTTG GATCATGCTT GGTATCTGAT CTCCATAAAT ACGTCTCACA TATTGTTCCC 
AAGGTCAAAA GTGATCACGC CGAAAGATGG GAACAGTTGG ATTTGGTAAT TACGGAGGAG 
TTTGATCAGA TCATCAGGAT GGTTCGTGTT CCGACTTTAA CAACTGATCC TATGGTACCT 
TTGGATTGGT GGAGCAGATA TTATGCATCG ATGTCACAAT TTCATAGATC ACCGGGATAT 
CCAGAATCTG GCATGGAGTA TGTACGTATC TTCCGCCGAC CACTTGAACA GATGAACGGA 
TACAACAACT TCTCTGATTT CCTGGACACA TTCCCGTTTG TCAAATCAAT GAAGGGAGAT 
TTCGACGATC CGGAAGAAAA AGAAAAGGCT GGAGAATTGA AATGCCGACT TCTCATCTCG 
AAACTCAAGA AGGGTAAGCC TCCAGCTGCG ATAAATACCG TTGTCGACTT CGTTGGACCA 
ACTCGATGTC TCGTCCGAGT TTACATCATC GAAGCAAATG GGCTGATCTC AAATGCACGG 
AAAGGACGAG TCGACTCGTA CGTTAAACTT CATTGCGGAA AGCAGAACGT GAACCTGAAG 
AAGAACTACC GCTCAGAGTG TTGTGATCCG ATATTTGGAG AACGAGTTGA CATGACGGTT 
ACGATTCCGC TGGAGAAAGA TCTGAAAATC ACTGTGATGG GCAAGCGGAG AATCCTTTCG 
GATCAAGAAA TAGGATCAAC TACAATCGAT CTGGAAAATC GTCTTCTCAC AAAATGGAGA 
GCAACTGGTG GGCTGAGTGG TCAGTTCACT GTACAAGGAG AGCTGCAATG GAGGGATCAG 
ATGACTCCAA TGGAGATCCT GAAGTCGTAC TGCTACAAGA TGATGCTATC AGTTCCAAAG 
ATTGAAAGTA GGCAGACGGA GAGAGGAGAA GAGAAGGGAA TTACCATTGA GAAGATCACA 
TTCTGGTTCA GTGAAGTTCT CCATGTCTTT GAGAACGAAG AGATTGCTAT GCTGAATTCT 
CAACGTCAAA AAGCTGGAAA AGAAAATTTC AGTGATGGTT CTGATCAACA AAATGAGGAC 
GTATCAGACG GTAGTTGGGA TGAAGAGGAT CTAGAACGAG AGAAAGAGAA GCTAAAGTGG 
GAGAAGCATC GGAGCAAGGG CAAGCCATTG AAGAAGGTTA CAACGGAAAA GGCAGAAACG 
GCTGATGGAG AGTTGAGGAA GAAGGCGAAG ATGAGAATAA TGGGATCTCA GTTGCAAACA 
ATTGCTCTCT TCATTCTCCG ACAGATGAAT TTGGTCCCAG AGCATGTCGA ATCTCGGCCA 
TTGTTCTCGG ATAAAGGAGG AAGAACTCAG AAGGGAGAAC TTCGAATGTT CGTTGACATC 
TTTCCGATGG AATATGGCGC GATTCCTGCT CCATTCAACA TTGCACCACG GAAACCCATC 
AACTATCAGC TACGTATCGC GGTCATGGAT GTACGAGGAG CGATTCCAGT CAAAAGAAGC 
TTTGCCGAGC CTGTCTCCGA TCTTTACGTC AAGGCATTCA TCAATGGAAT GACTAAGGGA 
CACAAGACGG ATACGCATTT CAGAGTTCTC GATGGAACCG GGGAGTTCAA CTGGCGTTTC 
CTGCTCAACT TCGACTACAA TCCTTGGGAG AAGAAGGTGG TCGCGTACAC CAAAAACCGA 
TTCTTCTGCA AACCAGTGGA AGAACTCGTC GATCCGATTC TTGTCATTGA GCTTTGGGAT 
AAGAACAAGT TCCGCAAGGA TCGTCTTCTC GGTGACATTG AACTGGATCT TCTCGATTTC 
ATTGAGGGAA TCGGTTCTCC ATCCGACGTT GGTGTCTACT CAACAAAGAA GAGACAGCGT 
GGAGTGAAGT GTCCAAAGTG TTGTACCAGG AGAGGATGTC TCTGCAAATG CTGTATCTTC 
TGCTTTGAGA CCAAGTGCTT GTGTGGAAAA CGCAAAGTGA AGAAGAAGCC ATTCCCGAAG 
CCTGTGTTGT TCGTGGAACC AGAAGGTTAC GATGATACTG TGAACATCTT CGAATCTCGC 
AACTTGTACG GTTGGTGGCC AATGCTTACC GAGGAGTATC CACACGAGGA ACCTCAGAAT 
GCCAAGAAGA AGAATGATGA CGTTGGAAAG GATCCTAAAT GGATCATGGG TCTTGTTGAG 
ATGGACATGC TACTTCTCAC CAAACAAGAA GCCGATCAAG AGCCAGCTGG AAAGAAACGA 
TCCGAGCCGA ACCACAGCCC ATTCTTGGAA AAACCTGATC GGAAGTCATG GGCCAACTCG 
TGGTTGGTGT CCCGAATCAA GCCGTGCATC AAGTATTTCT GGCACTACTA TGGTCTTCAA 
ATTCTGCTCT GGCTCATCAT TATTGTTATT CTGATTCTCA CAATTTTCGT GTTGTTGCAC 
ACTTGGCCGA CAATTTTAGC CGAAATTATA AAGGCAATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492337.1
CDS	1..6105
Translation	MTVKEKLLKVKQKFKRGDSSSDVDSGPERDRDEDDSDNESRNTSKTSKSSKKSKSGKSTGTSEMTELSDDGGSDIELLPDDGKSFGKRKLQDTDKPCTWNVLVRIIEAKDVSTGSARVRTIFDGNSKMTRTVTHAIPKWRQNILYTIKNQPLEKLAAKVLTLRLVRPTALGESSVGEFSCYLSEVIHSPDRSVIAKWVALGFPGMDDEEPDDIAYENCGFLKVTLSVYRIDESPPTLIDDDGKEQIWSGAHLIDYTLKIRVFSVEQLIRQMINERKIKKKQRFYVMVQCGAHKTETTMESAFLDEDANIASVKFEQEIYLPIQWPTVISEIIFSLFDKKGRGKTCIGKATLPMKMIYEPGETGYLPTFGPAFLNVFDCERVTRFSIFSRKMRGSQQDGSRFVGRLFIAIDCVEYMEKANAQIMHLDHSPIMEAESICKNLEWYNCFCSMSGLNMINPQFASDPVCIMMSIGSFGSTSNEFRSCNSSTLPAEPNWDGCKYFTMPWGNLRPVAEVNAPWEVIEHRIEMSNVLMKMTNMLDTMIWEVRRIGNKAIDHVSSVGMEALECLEQQIDSASKYLGRINPVSSNALDRHILDYRKEKILKLREHFEKEAFSIDYSDGEVDAKLLRMLLKIRSMTLELAEDVQMTIPPVLIKMMSHGKLIGFAKIPISEIFQSDDEAQSGEWCGRTRAINIQWPTLVDQRNRKREHVAVLHAKMWFGRTDQLTKWKEHVQPADIRRFMEMYEVQTKGLALKWKDEADIYDGKWEKTNEVPLENGWNAVGHWIVMNTRHMFVPKLGQHTVHDKAFEIQKRTEDGTWKHFKYTDCYADELKPKDLDKHAKGWEVGSWAQDKFRNNGDEKGWVYSTNGVFFGSGVLTDREEKVHHNFRKRCIKRPRKHEGYNKELEDFEHFRTTMGNENWEYSPSKKEGPYHDLEDRTDRIRRRRYVREVENKDPDSEDPRFRVYEYQMQTGKWQLRCYIMWGNDLLPVVKNSSRAFVRISFAQYSKQTLLVDNSQNPIWNETVMFKSVLIAGGTRDIMKYPPVVSVEVVGECSNNEEANLGHFETKPNVICGNTDVRGTPQWFPLKFSNGRTRGAVLACFELYSEEDKDLIPLEPKCKHNYKERSEIPTEFRPQFDKFHVQFLCWGVRNLKKHKLLAVRRPFVGLTIGDQEFTLQPLKDVRRDPNFPEPMIVFGEVILPSALELSPPLIINLFDARAFNRQPLVGSCLVSDLHKYVSHIVPKVKSDHAERWEQLDLVITEEFDQIIRMVRVPTLTTDPMVPLDWWSRYYASMSQFHRSPGYPESGMEYVRIFRRPLEQMNGYNNFSDFLDTFPFVKSMKGDFDDPEEKEKAGELKCRLLISKLKKGKPPAAINTVVDFVGPTRCLVRVYIIEANGLISNARKGRVDSYVKLHCGKQNVNLKKNYRSECCDPIFGERVDMTVTIPLEKDLKITVMGKRRILSDQEIGSTTIDLENRLLTKWRATGGLSGQFTVQGELQWRDQMTPMEILKSYCYKMMLSVPKIESRQTERGEEKGITIEKITFWFSEVLHVFENEEIAMLNSQRQKAGKENFSDGSDQQNEDVSDGSWDEEDLEREKEKLKWEKHRSKGKPLKKVTTEKAETADGELRKKAKMRIMGSQLQTIALFILRQMNLVPEHVESRPLFSDKGGRTQKGELRMFVDIFPMEYGAIPAPFNIAPRKPINYQLRIAVMDVRGAIPVKRSFAEPVSDLYVKAFINGMTKGHKTDTHFRVLDGTGEFNWRFLLNFDYNPWEKKVVAYTKNRFFCKPVEELVDPILVIELWDKNKFRKDRLLGDIELDLLDFIEGIGSPSDVGVYSTKKRQRGVKCPKCCTRRGCLCKCCIFCFETKCLCGKRKVKKKPFPKPVLFVEPEGYDDTVNIFESRNLYGWWPMLTEEYPHEEPQNAKKKNDDVGKDPKWIMGLVEMDMLLLTKQEADQEPAGKKRSEPNHSPFLEKPDRKSWANSWLVSRIKPCIKYFWHYYGLQILLWLIIIVILILTIFVLLHTWPTILAEIIKAIF

Target ORF information:

RefSeq Version	NM_059936.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Sperm vesicle fusion protein fer-1 (fer-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059936.3

ORF Insert Sequence:

ATGACGGTCA AGGAAAAGCT ACTGAAAGTG AAGCAAAAGT TTAAGCGAGG GGATTCATCC 
AGTGATGTGG ATTCCGGCCC GGAGAGAGAT CGGGATGAAG ACGATTCGGA TAACGAATCG 
AGGAATACAA GCAAGACGAG CAAATCCAGC AAAAAGAGCA AATCGGGAAA GAGCACGGGA 
ACATCGGAGA TGACTGAGCT ATCGGACGAC GGTGGATCTG ACATCGAGCT GCTGCCAGAT 
GATGGAAAAA GCTTTGGAAA ACGGAAACTG CAGGACACTG ACAAGCCATG TACCTGGAAT 
GTTCTTGTAC GAATTATTGA GGCAAAGGAT GTCAGCACAG GATCCGCAAG AGTTCGAACG 
ATCTTTGACG GAAATTCAAA AATGACAAGA ACAGTTACTC ATGCAATTCC AAAGTGGCGA 
CAAAATATTC TCTACACAAT AAAGAACCAG CCACTGGAGA AGCTTGCTGC CAAAGTTTTG 
ACTCTTCGGC TCGTGCGTCC TACAGCACTT GGTGAATCAA GCGTCGGAGA GTTCAGCTGC 
TACCTCTCGG AAGTAATTCA CTCTCCCGAC AGATCTGTCA TTGCAAAATG GGTCGCTCTG 
GGATTTCCTG GAATGGATGA CGAGGAACCG GACGATATCG CCTATGAGAA CTGTGGATTC 
CTGAAGGTGA CGCTCTCTGT GTACCGGATC GACGAGTCAC CACCAACGCT GATTGATGAC 
GATGGGAAGG AGCAAATCTG GAGTGGAGCA CATCTCATTG ATTATACTCT TAAGATCAGA 
GTGTTCTCCG TGGAACAGCT CATCCGACAG ATGATCAATG AGAGGAAAAT CAAGAAGAAG 
CAAAGATTCT ATGTGATGGT TCAGTGTGGA GCACACAAGA CCGAAACAAC AATGGAATCT 
GCGTTTCTTG ATGAAGATGC AAACATCGCA TCAGTGAAGT TTGAGCAAGA GATCTATCTT 
CCAATTCAAT GGCCAACTGT CATCTCAGAG ATTATTTTCT CATTGTTCGA CAAAAAGGGA 
CGAGGCAAAA CGTGCATAGG AAAAGCCACT CTTCCAATGA AAATGATTTA CGAGCCAGGA 
GAGACTGGAT ACCTTCCGAC TTTTGGACCT GCGTTTCTCA ATGTATTCGA TTGCGAACGC 
GTGACTCGAT TCAGCATCTT CTCAAGAAAA ATGCGAGGAT CCCAGCAGGA TGGGAGCAGA 
TTTGTTGGGC GTTTGTTCAT TGCGATCGAT TGTGTAGAGT ATATGGAGAA GGCTAATGCT 
CAGATCATGC ATCTTGATCA TTCCCCAATT ATGGAAGCCG AATCGATTTG TAAAAATCTC 
GAATGGTACA ACTGCTTCTG TTCCATGAGT GGCCTCAACA TGATCAATCC TCAATTCGCA 
TCCGATCCAG TCTGCATAAT GATGTCAATC GGTTCATTTG GAAGCACGAG CAACGAGTTC 
AGATCATGCA ATAGCTCAAC ACTGCCAGCA GAGCCAAACT GGGATGGATG CAAGTACTTC 
ACGATGCCAT GGGGAAATCT CCGACCAGTT GCCGAAGTCA ATGCTCCGTG GGAAGTTATT 
GAGCATCGGA TTGAAATGAG CAATGTTCTC ATGAAAATGA CGAATATGCT TGATACGATG 
ATTTGGGAGG TTCGACGCAT TGGAAACAAA GCAATCGACC ATGTTTCGTC AGTTGGAATG 
GAAGCTTTGG AGTGCTTGGA GCAGCAAATT GATTCGGCTA GCAAGTATTT GGGACGCATC 
AACCCCGTTA GCAGCAACGC ACTGGATCGC CATATCCTTG ACTACCGGAA GGAAAAGATC 
TTGAAGCTGC GAGAGCATTT TGAGAAGGAA GCCTTCAGTA TAGATTATTC TGATGGAGAA 
GTCGATGCGA AGCTGCTCCG AATGCTTCTA AAAATTCGAT CGATGACTCT TGAGTTGGCA 
GAAGATGTTC AAATGACCAT TCCACCCGTT CTCATCAAAA TGATGTCCCA TGGGAAGTTG 
ATCGGTTTCG CCAAAATCCC GATTTCGGAG ATTTTCCAAT CGGATGATGA AGCTCAATCA 
GGAGAATGGT GTGGGAGGAC CCGTGCTATA AATATTCAAT GGCCGACTTT GGTGGATCAG 
AGAAACAGGA AAAGAGAGCA CGTCGCAGTA CTCCATGCAA AGATGTGGTT TGGACGTACA 
GATCAGCTCA CAAAATGGAA GGAGCACGTA CAACCCGCAG ACATCCGTCG TTTCATGGAA 
ATGTACGAAG TGCAGACGAA GGGACTCGCA TTGAAATGGA AAGATGAAGC TGATATTTAC 
GATGGGAAAT GGGAGAAGAC GAATGAAGTG CCTCTCGAAA ATGGATGGAA TGCTGTTGGT 
CACTGGATTG TGATGAATAC TCGTCATATG TTTGTACCGA AACTTGGACA ACACACGGTT 
CATGACAAGG CTTTTGAAAT CCAGAAGAGA ACTGAAGACG GAACGTGGAA ACACTTTAAG 
TACACGGATT GCTACGCCGA CGAGTTGAAA CCCAAGGATC TTGACAAACA TGCGAAAGGA 
TGGGAAGTAG GCTCTTGGGC TCAAGACAAG TTTCGAAACA ATGGAGACGA AAAAGGATGG 
GTGTACTCGA CGAATGGTGT CTTTTTTGGA AGCGGAGTCC TGACAGACCG AGAAGAAAAA 
GTTCACCACA ACTTCAGAAA GCGTTGTATC AAGAGGCCAA GGAAGCATGA AGGCTATAAC 
AAGGAGTTGG AGGACTTTGA GCATTTTCGG ACAACGATGG GAAATGAGAA TTGGGAGTAC 
TCCCCCAGCA AGAAGGAAGG CCCATACCAC GATTTGGAAG ACCGAACCGA TCGGATCAGA 
CGTAGACGAT ATGTACGAGA AGTTGAGAAC AAAGACCCGG ACAGTGAAGA TCCAAGATTT 
CGCGTTTACG AATATCAAAT GCAAACTGGA AAATGGCAAC TGAGATGCTA TATCATGTGG 
GGAAACGATT TGCTGCCAGT TGTCAAGAAC TCGTCTCGGG CATTTGTTCG CATTTCGTTT 
GCACAATACT CAAAGCAGAC GCTTCTCGTG GACAACTCTC AAAATCCAAT TTGGAATGAG 
ACAGTCATGT TCAAGTCAGT TCTGATCGCT GGTGGAACTC GAGATATCAT GAAATACCCG 
CCAGTTGTCT CTGTTGAAGT CGTTGGCGAG TGCTCAAACA ATGAGGAGGC AAACCTCGGA 
CACTTCGAAA CCAAACCAAA CGTCATTTGC GGAAATACGG ATGTTCGTGG CACGCCACAA 
TGGTTTCCGT TGAAGTTCTC CAATGGACGT ACTCGTGGAG CGGTTCTTGC ATGCTTCGAG 
CTTTACTCAG AAGAAGATAA AGATTTGATT CCACTGGAGC CAAAATGCAA ACACAATTAT 
AAGGAGAGAA GTGAGATCCC AACAGAGTTC AGACCACAGT TCGATAAATT CCACGTGCAA 
TTTTTATGCT GGGGTGTGAG AAATCTCAAG AAGCATAAGC TTCTCGCCGT ACGTCGCCCA 
TTTGTTGGAC TGACAATTGG GGATCAAGAG TTCACATTGC AACCTCTCAA GGATGTCCGA 
AGAGATCCGA ACTTCCCTGA GCCCATGATT GTTTTTGGAG AAGTAATCCT ACCATCTGCT 
CTTGAGCTAT CACCTCCACT AATCATCAAT CTCTTCGATG CTAGGGCCTT CAATCGTCAG 
CCATTAGTTG GATCATGCTT GGTATCTGAT CTCCATAAAT ACGTCTCACA TATTGTTCCC 
AAGGTCAAAA GTGATCACGC CGAAAGATGG GAACAGTTGG ATTTGGTAAT TACGGAGGAG 
TTTGATCAGA TCATCAGGAT GGTTCGTGTT CCGACTTTAA CAACTGATCC TATGGTACCT 
TTGGATTGGT GGAGCAGATA TTATGCATCG ATGTCACAAT TTCATAGATC ACCGGGATAT 
CCAGAATCTG GCATGGAGTA TGTACGTATC TTCCGCCGAC CACTTGAACA GATGAACGGA 
TACAACAACT TCTCTGATTT CCTGGACACA TTCCCGTTTG TCAAATCAAT GAAGGGAGAT 
TTCGACGATC CGGAAGAAAA AGAAAAGGCT GGAGAATTGA AATGCCGACT TCTCATCTCG 
AAACTCAAGA AGGGTAAGCC TCCAGCTGCG ATAAATACCG TTGTCGACTT CGTTGGACCA 
ACTCGATGTC TCGTCCGAGT TTACATCATC GAAGCAAATG GGCTGATCTC AAATGCACGG 
AAAGGACGAG TCGACTCGTA CGTTAAACTT CATTGCGGAA AGCAGAACGT GAACCTGAAG 
AAGAACTACC GCTCAGAGTG TTGTGATCCG ATATTTGGAG AACGAGTTGA CATGACGGTT 
ACGATTCCGC TGGAGAAAGA TCTGAAAATC ACTGTGATGG GCAAGCGGAG AATCCTTTCG 
GATCAAGAAA TAGGATCAAC TACAATCGAT CTGGAAAATC GTCTTCTCAC AAAATGGAGA 
GCAACTGGTG GGCTGAGTGG TCAGTTCACT GTACAAGGAG AGCTGCAATG GAGGGATCAG 
ATGACTCCAA TGGAGATCCT GAAGTCGTAC TGCTACAAGA TGATGCTATC AGTTCCAAAG 
ATTGAAAGTA GGCAGACGGA GAGAGGAGAA GAGAAGGGAA TTACCATTGA GAAGATCACA 
TTCTGGTTCA GTGAAGTTCT CCATGTCTTT GAGAACGAAG AGATTGCTAT GCTGAATTCT 
CAACGTCAAA AAGCTGGAAA AGAAAATTTC AGTGATGGTT CTGATCAACA AAATGAGGAC 
GTATCAGACG GTAGTTGGGA TGAAGAGGAT CTAGAACGAG AGAAAGAGAA GCTAAAGTGG 
GAGAAGCATC GGAGCAAGGG CAAGCCATTG AAGAAGGTTA CAACGGAAAA GGCAGAAACG 
GCTGATGGAG AGTTGAGGAA GAAGGCGAAG ATGAGAATAA TGGGATCTCA GTTGCAAACA 
ATTGCTCTCT TCATTCTCCG ACAGATGAAT TTGGTCCCAG AGCATGTCGA ATCTCGGCCA 
TTGTTCTCGG ATAAAGGAGG AAGAACTCAG AAGGGAGAAC TTCGAATGTT CGTTGACATC 
TTTCCGATGG AATATGGCGC GATTCCTGCT CCATTCAACA TTGCACCACG GAAACCCATC 
AACTATCAGC TACGTATCGC GGTCATGGAT GTACGAGGAG CGATTCCAGT CAAAAGAAGC 
TTTGCCGAGC CTGTCTCCGA TCTTTACGTC AAGGCATTCA TCAATGGAAT GACTAAGGGA 
CACAAGACGG ATACGCATTT CAGAGTTCTC GATGGAACCG GGGAGTTCAA CTGGCGTTTC 
CTGCTCAACT TCGACTACAA TCCTTGGGAG AAGAAGGTGG TCGCGTACAC CAAAAACCGA 
TTCTTCTGCA AACCAGTGGA AGAACTCGTC GATCCGATTC TTGTCATTGA GCTTTGGGAT 
AAGAACAAGT TCCGCAAGGA TCGTCTTCTC GGTGACATTG AACTGGATCT TCTCGATTTC 
ATTGAGGGAA TCGGTTCTCC ATCCGACGTT GGTGTCTACT CAACAAAGAA GAGACAGCGT 
GGAGTGAAGT GTCCAAAGTG TTGTACCAGG AGAGGATGTC TCTGCAAATG CTGTATCTTC 
TGCTTTGAGA CCAAGTGCTT GTGTGGAAAA CGCAAAGTGA AGAAGAAGCC ATTCCCGAAG 
CCTGTGTTGT TCGTGGAACC AGAAGGTTAC GATGATACTG TGAACATCTT CGAATCTCGC 
AACTTGTACG GTTGGTGGCC AATGCTTACC GAGGAGTATC CACACGAGGA ACCTCAGAAT 
GCCAAGAAGA AGAATGATGA CGTTGGAAAG GATCCTAAAT GGATCATGGG TCTTGTTGAG 
ATGGACATGC TACTTCTCAC CAAACAAGAA GCCGATCAAG AGCCAGCTGG AAAGAAACGA 
TCCGAGCCGA ACCACAGCCC ATTCTTGGAA AAACCTGATC GGAAGTCATG GGCCAACTCG 
TGGTTGGTGT CCCGAATCAA GCCGTGCATC AAGTATTTCT GGCACTACTA TGGTCTTCAA 
ATTCTGCTCT GGCTCATCAT TATTGTTATT CTGATTCTCA CAATTTTCGT GTTGTTGCAC 
ACTTGGCCGA CAATTTTAGC CGAAATTATA AAGGCAATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fer-1 ( NM_059936.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492337.1 Caenorhabditis elegans Sperm vesicle fusion protein fer-1 (fer-1), mRNA.

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CloneID	OCa335673
Clone ID Related Accession (Same CDS sequence)	NM_059936.3 , NM_059936.4
Accession Version	NM_059936.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Sperm vesicle fusion protein fer-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059936.3.

ATGACGGTCA AGGAAAAGCT ACTGAAAGTG AAGCAAAAGT TTAAGCGAGG GGATTCATCC 
AGTGATGTGG ATTCCGGCCC GGAGAGAGAT CGGGATGAAG ACGATTCGGA TAACGAATCG 
AGGAATACAA GCAAGACGAG CAAATCCAGC AAAAAGAGCA AATCGGGAAA GAGCACGGGA 
ACATCGGAGA TGACTGAGCT ATCGGACGAC GGTGGATCTG ACATCGAGCT GCTGCCAGAT 
GATGGAAAAA GCTTTGGAAA ACGGAAACTG CAGGACACTG ACAAGCCATG TACCTGGAAT 
GTTCTTGTAC GAATTATTGA GGCAAAGGAT GTCAGCACAG GATCCGCAAG AGTTCGAACG 
ATCTTTGACG GAAATTCAAA AATGACAAGA ACAGTTACTC ATGCAATTCC AAAGTGGCGA 
CAAAATATTC TCTACACAAT AAAGAACCAG CCACTGGAGA AGCTTGCTGC CAAAGTTTTG 
ACTCTTCGGC TCGTGCGTCC TACAGCACTT GGTGAATCAA GCGTCGGAGA GTTCAGCTGC 
TACCTCTCGG AAGTAATTCA CTCTCCCGAC AGATCTGTCA TTGCAAAATG GGTCGCTCTG 
GGATTTCCTG GAATGGATGA CGAGGAACCG GACGATATCG CCTATGAGAA CTGTGGATTC 
CTGAAGGTGA CGCTCTCTGT GTACCGGATC GACGAGTCAC CACCAACGCT GATTGATGAC 
GATGGGAAGG AGCAAATCTG GAGTGGAGCA CATCTCATTG ATTATACTCT TAAGATCAGA 
GTGTTCTCCG TGGAACAGCT CATCCGACAG ATGATCAATG AGAGGAAAAT CAAGAAGAAG 
CAAAGATTCT ATGTGATGGT TCAGTGTGGA GCACACAAGA CCGAAACAAC AATGGAATCT 
GCGTTTCTTG ATGAAGATGC AAACATCGCA TCAGTGAAGT TTGAGCAAGA GATCTATCTT 
CCAATTCAAT GGCCAACTGT CATCTCAGAG ATTATTTTCT CATTGTTCGA CAAAAAGGGA 
CGAGGCAAAA CGTGCATAGG AAAAGCCACT CTTCCAATGA AAATGATTTA CGAGCCAGGA 
GAGACTGGAT ACCTTCCGAC TTTTGGACCT GCGTTTCTCA ATGTATTCGA TTGCGAACGC 
GTGACTCGAT TCAGCATCTT CTCAAGAAAA ATGCGAGGAT CCCAGCAGGA TGGGAGCAGA 
TTTGTTGGGC GTTTGTTCAT TGCGATCGAT TGTGTAGAGT ATATGGAGAA GGCTAATGCT 
CAGATCATGC ATCTTGATCA TTCCCCAATT ATGGAAGCCG AATCGATTTG TAAAAATCTC 
GAATGGTACA ACTGCTTCTG TTCCATGAGT GGCCTCAACA TGATCAATCC TCAATTCGCA 
TCCGATCCAG TCTGCATAAT GATGTCAATC GGTTCATTTG GAAGCACGAG CAACGAGTTC 
AGATCATGCA ATAGCTCAAC ACTGCCAGCA GAGCCAAACT GGGATGGATG CAAGTACTTC 
ACGATGCCAT GGGGAAATCT CCGACCAGTT GCCGAAGTCA ATGCTCCGTG GGAAGTTATT 
GAGCATCGGA TTGAAATGAG CAATGTTCTC ATGAAAATGA CGAATATGCT TGATACGATG 
ATTTGGGAGG TTCGACGCAT TGGAAACAAA GCAATCGACC ATGTTTCGTC AGTTGGAATG 
GAAGCTTTGG AGTGCTTGGA GCAGCAAATT GATTCGGCTA GCAAGTATTT GGGACGCATC 
AACCCCGTTA GCAGCAACGC ACTGGATCGC CATATCCTTG ACTACCGGAA GGAAAAGATC 
TTGAAGCTGC GAGAGCATTT TGAGAAGGAA GCCTTCAGTA TAGATTATTC TGATGGAGAA 
GTCGATGCGA AGCTGCTCCG AATGCTTCTA AAAATTCGAT CGATGACTCT TGAGTTGGCA 
GAAGATGTTC AAATGACCAT TCCACCCGTT CTCATCAAAA TGATGTCCCA TGGGAAGTTG 
ATCGGTTTCG CCAAAATCCC GATTTCGGAG ATTTTCCAAT CGGATGATGA AGCTCAATCA 
GGAGAATGGT GTGGGAGGAC CCGTGCTATA AATATTCAAT GGCCGACTTT GGTGGATCAG 
AGAAACAGGA AAAGAGAGCA CGTCGCAGTA CTCCATGCAA AGATGTGGTT TGGACGTACA 
GATCAGCTCA CAAAATGGAA GGAGCACGTA CAACCCGCAG ACATCCGTCG TTTCATGGAA 
ATGTACGAAG TGCAGACGAA GGGACTCGCA TTGAAATGGA AAGATGAAGC TGATATTTAC 
GATGGGAAAT GGGAGAAGAC GAATGAAGTG CCTCTCGAAA ATGGATGGAA TGCTGTTGGT 
CACTGGATTG TGATGAATAC TCGTCATATG TTTGTACCGA AACTTGGACA ACACACGGTT 
CATGACAAGG CTTTTGAAAT CCAGAAGAGA ACTGAAGACG GAACGTGGAA ACACTTTAAG 
TACACGGATT GCTACGCCGA CGAGTTGAAA CCCAAGGATC TTGACAAACA TGCGAAAGGA 
TGGGAAGTAG GCTCTTGGGC TCAAGACAAG TTTCGAAACA ATGGAGACGA AAAAGGATGG 
GTGTACTCGA CGAATGGTGT CTTTTTTGGA AGCGGAGTCC TGACAGACCG AGAAGAAAAA 
GTTCACCACA ACTTCAGAAA GCGTTGTATC AAGAGGCCAA GGAAGCATGA AGGCTATAAC 
AAGGAGTTGG AGGACTTTGA GCATTTTCGG ACAACGATGG GAAATGAGAA TTGGGAGTAC 
TCCCCCAGCA AGAAGGAAGG CCCATACCAC GATTTGGAAG ACCGAACCGA TCGGATCAGA 
CGTAGACGAT ATGTACGAGA AGTTGAGAAC AAAGACCCGG ACAGTGAAGA TCCAAGATTT 
CGCGTTTACG AATATCAAAT GCAAACTGGA AAATGGCAAC TGAGATGCTA TATCATGTGG 
GGAAACGATT TGCTGCCAGT TGTCAAGAAC TCGTCTCGGG CATTTGTTCG CATTTCGTTT 
GCACAATACT CAAAGCAGAC GCTTCTCGTG GACAACTCTC AAAATCCAAT TTGGAATGAG 
ACAGTCATGT TCAAGTCAGT TCTGATCGCT GGTGGAACTC GAGATATCAT GAAATACCCG 
CCAGTTGTCT CTGTTGAAGT CGTTGGCGAG TGCTCAAACA ATGAGGAGGC AAACCTCGGA 
CACTTCGAAA CCAAACCAAA CGTCATTTGC GGAAATACGG ATGTTCGTGG CACGCCACAA 
TGGTTTCCGT TGAAGTTCTC CAATGGACGT ACTCGTGGAG CGGTTCTTGC ATGCTTCGAG 
CTTTACTCAG AAGAAGATAA AGATTTGATT CCACTGGAGC CAAAATGCAA ACACAATTAT 
AAGGAGAGAA GTGAGATCCC AACAGAGTTC AGACCACAGT TCGATAAATT CCACGTGCAA 
TTTTTATGCT GGGGTGTGAG AAATCTCAAG AAGCATAAGC TTCTCGCCGT ACGTCGCCCA 
TTTGTTGGAC TGACAATTGG GGATCAAGAG TTCACATTGC AACCTCTCAA GGATGTCCGA 
AGAGATCCGA ACTTCCCTGA GCCCATGATT GTTTTTGGAG AAGTAATCCT ACCATCTGCT 
CTTGAGCTAT CACCTCCACT AATCATCAAT CTCTTCGATG CTAGGGCCTT CAATCGTCAG 
CCATTAGTTG GATCATGCTT GGTATCTGAT CTCCATAAAT ACGTCTCACA TATTGTTCCC 
AAGGTCAAAA GTGATCACGC CGAAAGATGG GAACAGTTGG ATTTGGTAAT TACGGAGGAG 
TTTGATCAGA TCATCAGGAT GGTTCGTGTT CCGACTTTAA CAACTGATCC TATGGTACCT 
TTGGATTGGT GGAGCAGATA TTATGCATCG ATGTCACAAT TTCATAGATC ACCGGGATAT 
CCAGAATCTG GCATGGAGTA TGTACGTATC TTCCGCCGAC CACTTGAACA GATGAACGGA 
TACAACAACT TCTCTGATTT CCTGGACACA TTCCCGTTTG TCAAATCAAT GAAGGGAGAT 
TTCGACGATC CGGAAGAAAA AGAAAAGGCT GGAGAATTGA AATGCCGACT TCTCATCTCG 
AAACTCAAGA AGGGTAAGCC TCCAGCTGCG ATAAATACCG TTGTCGACTT CGTTGGACCA 
ACTCGATGTC TCGTCCGAGT TTACATCATC GAAGCAAATG GGCTGATCTC AAATGCACGG 
AAAGGACGAG TCGACTCGTA CGTTAAACTT CATTGCGGAA AGCAGAACGT GAACCTGAAG 
AAGAACTACC GCTCAGAGTG TTGTGATCCG ATATTTGGAG AACGAGTTGA CATGACGGTT 
ACGATTCCGC TGGAGAAAGA TCTGAAAATC ACTGTGATGG GCAAGCGGAG AATCCTTTCG 
GATCAAGAAA TAGGATCAAC TACAATCGAT CTGGAAAATC GTCTTCTCAC AAAATGGAGA 
GCAACTGGTG GGCTGAGTGG TCAGTTCACT GTACAAGGAG AGCTGCAATG GAGGGATCAG 
ATGACTCCAA TGGAGATCCT GAAGTCGTAC TGCTACAAGA TGATGCTATC AGTTCCAAAG 
ATTGAAAGTA GGCAGACGGA GAGAGGAGAA GAGAAGGGAA TTACCATTGA GAAGATCACA 
TTCTGGTTCA GTGAAGTTCT CCATGTCTTT GAGAACGAAG AGATTGCTAT GCTGAATTCT 
CAACGTCAAA AAGCTGGAAA AGAAAATTTC AGTGATGGTT CTGATCAACA AAATGAGGAC 
GTATCAGACG GTAGTTGGGA TGAAGAGGAT CTAGAACGAG AGAAAGAGAA GCTAAAGTGG 
GAGAAGCATC GGAGCAAGGG CAAGCCATTG AAGAAGGTTA CAACGGAAAA GGCAGAAACG 
GCTGATGGAG AGTTGAGGAA GAAGGCGAAG ATGAGAATAA TGGGATCTCA GTTGCAAACA 
ATTGCTCTCT TCATTCTCCG ACAGATGAAT TTGGTCCCAG AGCATGTCGA ATCTCGGCCA 
TTGTTCTCGG ATAAAGGAGG AAGAACTCAG AAGGGAGAAC TTCGAATGTT CGTTGACATC 
TTTCCGATGG AATATGGCGC GATTCCTGCT CCATTCAACA TTGCACCACG GAAACCCATC 
AACTATCAGC TACGTATCGC GGTCATGGAT GTACGAGGAG CGATTCCAGT CAAAAGAAGC 
TTTGCCGAGC CTGTCTCCGA TCTTTACGTC AAGGCATTCA TCAATGGAAT GACTAAGGGA 
CACAAGACGG ATACGCATTT CAGAGTTCTC GATGGAACCG GGGAGTTCAA CTGGCGTTTC 
CTGCTCAACT TCGACTACAA TCCTTGGGAG AAGAAGGTGG TCGCGTACAC CAAAAACCGA 
TTCTTCTGCA AACCAGTGGA AGAACTCGTC GATCCGATTC TTGTCATTGA GCTTTGGGAT 
AAGAACAAGT TCCGCAAGGA TCGTCTTCTC GGTGACATTG AACTGGATCT TCTCGATTTC 
ATTGAGGGAA TCGGTTCTCC ATCCGACGTT GGTGTCTACT CAACAAAGAA GAGACAGCGT 
GGAGTGAAGT GTCCAAAGTG TTGTACCAGG AGAGGATGTC TCTGCAAATG CTGTATCTTC 
TGCTTTGAGA CCAAGTGCTT GTGTGGAAAA CGCAAAGTGA AGAAGAAGCC ATTCCCGAAG 
CCTGTGTTGT TCGTGGAACC AGAAGGTTAC GATGATACTG TGAACATCTT CGAATCTCGC 
AACTTGTACG GTTGGTGGCC AATGCTTACC GAGGAGTATC CACACGAGGA ACCTCAGAAT 
GCCAAGAAGA AGAATGATGA CGTTGGAAAG GATCCTAAAT GGATCATGGG TCTTGTTGAG 
ATGGACATGC TACTTCTCAC CAAACAAGAA GCCGATCAAG AGCCAGCTGG AAAGAAACGA 
TCCGAGCCGA ACCACAGCCC ATTCTTGGAA AAACCTGATC GGAAGTCATG GGCCAACTCG 
TGGTTGGTGT CCCGAATCAA GCCGTGCATC AAGTATTTCT GGCACTACTA TGGTCTTCAA 
ATTCTGCTCT GGCTCATCAT TATTGTTATT CTGATTCTCA CAATTTTCGT GTTGTTGCAC 
ACTTGGCCGA CAATTTTAGC CGAAATTATA AAGGCAATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492337.1
CDS	19..6123
Translation	MTVKEKLLKVKQKFKRGDSSSDVDSGPERDRDEDDSDNESRNTSKTSKSSKKSKSGKSTGTSEMTELSDDGGSDIELLPDDGKSFGKRKLQDTDKPCTWNVLVRIIEAKDVSTGSARVRTIFDGNSKMTRTVTHAIPKWRQNILYTIKNQPLEKLAAKVLTLRLVRPTALGESSVGEFSCYLSEVIHSPDRSVIAKWVALGFPGMDDEEPDDIAYENCGFLKVTLSVYRIDESPPTLIDDDGKEQIWSGAHLIDYTLKIRVFSVEQLIRQMINERKIKKKQRFYVMVQCGAHKTETTMESAFLDEDANIASVKFEQEIYLPIQWPTVISEIIFSLFDKKGRGKTCIGKATLPMKMIYEPGETGYLPTFGPAFLNVFDCERVTRFSIFSRKMRGSQQDGSRFVGRLFIAIDCVEYMEKANAQIMHLDHSPIMEAESICKNLEWYNCFCSMSGLNMINPQFASDPVCIMMSIGSFGSTSNEFRSCNSSTLPAEPNWDGCKYFTMPWGNLRPVAEVNAPWEVIEHRIEMSNVLMKMTNMLDTMIWEVRRIGNKAIDHVSSVGMEALECLEQQIDSASKYLGRINPVSSNALDRHILDYRKEKILKLREHFEKEAFSIDYSDGEVDAKLLRMLLKIRSMTLELAEDVQMTIPPVLIKMMSHGKLIGFAKIPISEIFQSDDEAQSGEWCGRTRAINIQWPTLVDQRNRKREHVAVLHAKMWFGRTDQLTKWKEHVQPADIRRFMEMYEVQTKGLALKWKDEADIYDGKWEKTNEVPLENGWNAVGHWIVMNTRHMFVPKLGQHTVHDKAFEIQKRTEDGTWKHFKYTDCYADELKPKDLDKHAKGWEVGSWAQDKFRNNGDEKGWVYSTNGVFFGSGVLTDREEKVHHNFRKRCIKRPRKHEGYNKELEDFEHFRTTMGNENWEYSPSKKEGPYHDLEDRTDRIRRRRYVREVENKDPDSEDPRFRVYEYQMQTGKWQLRCYIMWGNDLLPVVKNSSRAFVRISFAQYSKQTLLVDNSQNPIWNETVMFKSVLIAGGTRDIMKYPPVVSVEVVGECSNNEEANLGHFETKPNVICGNTDVRGTPQWFPLKFSNGRTRGAVLACFELYSEEDKDLIPLEPKCKHNYKERSEIPTEFRPQFDKFHVQFLCWGVRNLKKHKLLAVRRPFVGLTIGDQEFTLQPLKDVRRDPNFPEPMIVFGEVILPSALELSPPLIINLFDARAFNRQPLVGSCLVSDLHKYVSHIVPKVKSDHAERWEQLDLVITEEFDQIIRMVRVPTLTTDPMVPLDWWSRYYASMSQFHRSPGYPESGMEYVRIFRRPLEQMNGYNNFSDFLDTFPFVKSMKGDFDDPEEKEKAGELKCRLLISKLKKGKPPAAINTVVDFVGPTRCLVRVYIIEANGLISNARKGRVDSYVKLHCGKQNVNLKKNYRSECCDPIFGERVDMTVTIPLEKDLKITVMGKRRILSDQEIGSTTIDLENRLLTKWRATGGLSGQFTVQGELQWRDQMTPMEILKSYCYKMMLSVPKIESRQTERGEEKGITIEKITFWFSEVLHVFENEEIAMLNSQRQKAGKENFSDGSDQQNEDVSDGSWDEEDLEREKEKLKWEKHRSKGKPLKKVTTEKAETADGELRKKAKMRIMGSQLQTIALFILRQMNLVPEHVESRPLFSDKGGRTQKGELRMFVDIFPMEYGAIPAPFNIAPRKPINYQLRIAVMDVRGAIPVKRSFAEPVSDLYVKAFINGMTKGHKTDTHFRVLDGTGEFNWRFLLNFDYNPWEKKVVAYTKNRFFCKPVEELVDPILVIELWDKNKFRKDRLLGDIELDLLDFIEGIGSPSDVGVYSTKKRQRGVKCPKCCTRRGCLCKCCIFCFETKCLCGKRKVKKKPFPKPVLFVEPEGYDDTVNIFESRNLYGWWPMLTEEYPHEEPQNAKKKNDDVGKDPKWIMGLVEMDMLLLTKQEADQEPAGKKRSEPNHSPFLEKPDRKSWANSWLVSRIKPCIKYFWHYYGLQILLWLIIIVILILTIFVLLHTWPTILAEIIKAIF

Target ORF information:

RefSeq Version	NM_059936.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Sperm vesicle fusion protein fer-1 (fer-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059936.4

ORF Insert Sequence:

ATGACGGTCA AGGAAAAGCT ACTGAAAGTG AAGCAAAAGT TTAAGCGAGG GGATTCATCC 
AGTGATGTGG ATTCCGGCCC GGAGAGAGAT CGGGATGAAG ACGATTCGGA TAACGAATCG 
AGGAATACAA GCAAGACGAG CAAATCCAGC AAAAAGAGCA AATCGGGAAA GAGCACGGGA 
ACATCGGAGA TGACTGAGCT ATCGGACGAC GGTGGATCTG ACATCGAGCT GCTGCCAGAT 
GATGGAAAAA GCTTTGGAAA ACGGAAACTG CAGGACACTG ACAAGCCATG TACCTGGAAT 
GTTCTTGTAC GAATTATTGA GGCAAAGGAT GTCAGCACAG GATCCGCAAG AGTTCGAACG 
ATCTTTGACG GAAATTCAAA AATGACAAGA ACAGTTACTC ATGCAATTCC AAAGTGGCGA 
CAAAATATTC TCTACACAAT AAAGAACCAG CCACTGGAGA AGCTTGCTGC CAAAGTTTTG 
ACTCTTCGGC TCGTGCGTCC TACAGCACTT GGTGAATCAA GCGTCGGAGA GTTCAGCTGC 
TACCTCTCGG AAGTAATTCA CTCTCCCGAC AGATCTGTCA TTGCAAAATG GGTCGCTCTG 
GGATTTCCTG GAATGGATGA CGAGGAACCG GACGATATCG CCTATGAGAA CTGTGGATTC 
CTGAAGGTGA CGCTCTCTGT GTACCGGATC GACGAGTCAC CACCAACGCT GATTGATGAC 
GATGGGAAGG AGCAAATCTG GAGTGGAGCA CATCTCATTG ATTATACTCT TAAGATCAGA 
GTGTTCTCCG TGGAACAGCT CATCCGACAG ATGATCAATG AGAGGAAAAT CAAGAAGAAG 
CAAAGATTCT ATGTGATGGT TCAGTGTGGA GCACACAAGA CCGAAACAAC AATGGAATCT 
GCGTTTCTTG ATGAAGATGC AAACATCGCA TCAGTGAAGT TTGAGCAAGA GATCTATCTT 
CCAATTCAAT GGCCAACTGT CATCTCAGAG ATTATTTTCT CATTGTTCGA CAAAAAGGGA 
CGAGGCAAAA CGTGCATAGG AAAAGCCACT CTTCCAATGA AAATGATTTA CGAGCCAGGA 
GAGACTGGAT ACCTTCCGAC TTTTGGACCT GCGTTTCTCA ATGTATTCGA TTGCGAACGC 
GTGACTCGAT TCAGCATCTT CTCAAGAAAA ATGCGAGGAT CCCAGCAGGA TGGGAGCAGA 
TTTGTTGGGC GTTTGTTCAT TGCGATCGAT TGTGTAGAGT ATATGGAGAA GGCTAATGCT 
CAGATCATGC ATCTTGATCA TTCCCCAATT ATGGAAGCCG AATCGATTTG TAAAAATCTC 
GAATGGTACA ACTGCTTCTG TTCCATGAGT GGCCTCAACA TGATCAATCC TCAATTCGCA 
TCCGATCCAG TCTGCATAAT GATGTCAATC GGTTCATTTG GAAGCACGAG CAACGAGTTC 
AGATCATGCA ATAGCTCAAC ACTGCCAGCA GAGCCAAACT GGGATGGATG CAAGTACTTC 
ACGATGCCAT GGGGAAATCT CCGACCAGTT GCCGAAGTCA ATGCTCCGTG GGAAGTTATT 
GAGCATCGGA TTGAAATGAG CAATGTTCTC ATGAAAATGA CGAATATGCT TGATACGATG 
ATTTGGGAGG TTCGACGCAT TGGAAACAAA GCAATCGACC ATGTTTCGTC AGTTGGAATG 
GAAGCTTTGG AGTGCTTGGA GCAGCAAATT GATTCGGCTA GCAAGTATTT GGGACGCATC 
AACCCCGTTA GCAGCAACGC ACTGGATCGC CATATCCTTG ACTACCGGAA GGAAAAGATC 
TTGAAGCTGC GAGAGCATTT TGAGAAGGAA GCCTTCAGTA TAGATTATTC TGATGGAGAA 
GTCGATGCGA AGCTGCTCCG AATGCTTCTA AAAATTCGAT CGATGACTCT TGAGTTGGCA 
GAAGATGTTC AAATGACCAT TCCACCCGTT CTCATCAAAA TGATGTCCCA TGGGAAGTTG 
ATCGGTTTCG CCAAAATCCC GATTTCGGAG ATTTTCCAAT CGGATGATGA AGCTCAATCA 
GGAGAATGGT GTGGGAGGAC CCGTGCTATA AATATTCAAT GGCCGACTTT GGTGGATCAG 
AGAAACAGGA AAAGAGAGCA CGTCGCAGTA CTCCATGCAA AGATGTGGTT TGGACGTACA 
GATCAGCTCA CAAAATGGAA GGAGCACGTA CAACCCGCAG ACATCCGTCG TTTCATGGAA 
ATGTACGAAG TGCAGACGAA GGGACTCGCA TTGAAATGGA AAGATGAAGC TGATATTTAC 
GATGGGAAAT GGGAGAAGAC GAATGAAGTG CCTCTCGAAA ATGGATGGAA TGCTGTTGGT 
CACTGGATTG TGATGAATAC TCGTCATATG TTTGTACCGA AACTTGGACA ACACACGGTT 
CATGACAAGG CTTTTGAAAT CCAGAAGAGA ACTGAAGACG GAACGTGGAA ACACTTTAAG 
TACACGGATT GCTACGCCGA CGAGTTGAAA CCCAAGGATC TTGACAAACA TGCGAAAGGA 
TGGGAAGTAG GCTCTTGGGC TCAAGACAAG TTTCGAAACA ATGGAGACGA AAAAGGATGG 
GTGTACTCGA CGAATGGTGT CTTTTTTGGA AGCGGAGTCC TGACAGACCG AGAAGAAAAA 
GTTCACCACA ACTTCAGAAA GCGTTGTATC AAGAGGCCAA GGAAGCATGA AGGCTATAAC 
AAGGAGTTGG AGGACTTTGA GCATTTTCGG ACAACGATGG GAAATGAGAA TTGGGAGTAC 
TCCCCCAGCA AGAAGGAAGG CCCATACCAC GATTTGGAAG ACCGAACCGA TCGGATCAGA 
CGTAGACGAT ATGTACGAGA AGTTGAGAAC AAAGACCCGG ACAGTGAAGA TCCAAGATTT 
CGCGTTTACG AATATCAAAT GCAAACTGGA AAATGGCAAC TGAGATGCTA TATCATGTGG 
GGAAACGATT TGCTGCCAGT TGTCAAGAAC TCGTCTCGGG CATTTGTTCG CATTTCGTTT 
GCACAATACT CAAAGCAGAC GCTTCTCGTG GACAACTCTC AAAATCCAAT TTGGAATGAG 
ACAGTCATGT TCAAGTCAGT TCTGATCGCT GGTGGAACTC GAGATATCAT GAAATACCCG 
CCAGTTGTCT CTGTTGAAGT CGTTGGCGAG TGCTCAAACA ATGAGGAGGC AAACCTCGGA 
CACTTCGAAA CCAAACCAAA CGTCATTTGC GGAAATACGG ATGTTCGTGG CACGCCACAA 
TGGTTTCCGT TGAAGTTCTC CAATGGACGT ACTCGTGGAG CGGTTCTTGC ATGCTTCGAG 
CTTTACTCAG AAGAAGATAA AGATTTGATT CCACTGGAGC CAAAATGCAA ACACAATTAT 
AAGGAGAGAA GTGAGATCCC AACAGAGTTC AGACCACAGT TCGATAAATT CCACGTGCAA 
TTTTTATGCT GGGGTGTGAG AAATCTCAAG AAGCATAAGC TTCTCGCCGT ACGTCGCCCA 
TTTGTTGGAC TGACAATTGG GGATCAAGAG TTCACATTGC AACCTCTCAA GGATGTCCGA 
AGAGATCCGA ACTTCCCTGA GCCCATGATT GTTTTTGGAG AAGTAATCCT ACCATCTGCT 
CTTGAGCTAT CACCTCCACT AATCATCAAT CTCTTCGATG CTAGGGCCTT CAATCGTCAG 
CCATTAGTTG GATCATGCTT GGTATCTGAT CTCCATAAAT ACGTCTCACA TATTGTTCCC 
AAGGTCAAAA GTGATCACGC CGAAAGATGG GAACAGTTGG ATTTGGTAAT TACGGAGGAG 
TTTGATCAGA TCATCAGGAT GGTTCGTGTT CCGACTTTAA CAACTGATCC TATGGTACCT 
TTGGATTGGT GGAGCAGATA TTATGCATCG ATGTCACAAT TTCATAGATC ACCGGGATAT 
CCAGAATCTG GCATGGAGTA TGTACGTATC TTCCGCCGAC CACTTGAACA GATGAACGGA 
TACAACAACT TCTCTGATTT CCTGGACACA TTCCCGTTTG TCAAATCAAT GAAGGGAGAT 
TTCGACGATC CGGAAGAAAA AGAAAAGGCT GGAGAATTGA AATGCCGACT TCTCATCTCG 
AAACTCAAGA AGGGTAAGCC TCCAGCTGCG ATAAATACCG TTGTCGACTT CGTTGGACCA 
ACTCGATGTC TCGTCCGAGT TTACATCATC GAAGCAAATG GGCTGATCTC AAATGCACGG 
AAAGGACGAG TCGACTCGTA CGTTAAACTT CATTGCGGAA AGCAGAACGT GAACCTGAAG 
AAGAACTACC GCTCAGAGTG TTGTGATCCG ATATTTGGAG AACGAGTTGA CATGACGGTT 
ACGATTCCGC TGGAGAAAGA TCTGAAAATC ACTGTGATGG GCAAGCGGAG AATCCTTTCG 
GATCAAGAAA TAGGATCAAC TACAATCGAT CTGGAAAATC GTCTTCTCAC AAAATGGAGA 
GCAACTGGTG GGCTGAGTGG TCAGTTCACT GTACAAGGAG AGCTGCAATG GAGGGATCAG 
ATGACTCCAA TGGAGATCCT GAAGTCGTAC TGCTACAAGA TGATGCTATC AGTTCCAAAG 
ATTGAAAGTA GGCAGACGGA GAGAGGAGAA GAGAAGGGAA TTACCATTGA GAAGATCACA 
TTCTGGTTCA GTGAAGTTCT CCATGTCTTT GAGAACGAAG AGATTGCTAT GCTGAATTCT 
CAACGTCAAA AAGCTGGAAA AGAAAATTTC AGTGATGGTT CTGATCAACA AAATGAGGAC 
GTATCAGACG GTAGTTGGGA TGAAGAGGAT CTAGAACGAG AGAAAGAGAA GCTAAAGTGG 
GAGAAGCATC GGAGCAAGGG CAAGCCATTG AAGAAGGTTA CAACGGAAAA GGCAGAAACG 
GCTGATGGAG AGTTGAGGAA GAAGGCGAAG ATGAGAATAA TGGGATCTCA GTTGCAAACA 
ATTGCTCTCT TCATTCTCCG ACAGATGAAT TTGGTCCCAG AGCATGTCGA ATCTCGGCCA 
TTGTTCTCGG ATAAAGGAGG AAGAACTCAG AAGGGAGAAC TTCGAATGTT CGTTGACATC 
TTTCCGATGG AATATGGCGC GATTCCTGCT CCATTCAACA TTGCACCACG GAAACCCATC 
AACTATCAGC TACGTATCGC GGTCATGGAT GTACGAGGAG CGATTCCAGT CAAAAGAAGC 
TTTGCCGAGC CTGTCTCCGA TCTTTACGTC AAGGCATTCA TCAATGGAAT GACTAAGGGA 
CACAAGACGG ATACGCATTT CAGAGTTCTC GATGGAACCG GGGAGTTCAA CTGGCGTTTC 
CTGCTCAACT TCGACTACAA TCCTTGGGAG AAGAAGGTGG TCGCGTACAC CAAAAACCGA 
TTCTTCTGCA AACCAGTGGA AGAACTCGTC GATCCGATTC TTGTCATTGA GCTTTGGGAT 
AAGAACAAGT TCCGCAAGGA TCGTCTTCTC GGTGACATTG AACTGGATCT TCTCGATTTC 
ATTGAGGGAA TCGGTTCTCC ATCCGACGTT GGTGTCTACT CAACAAAGAA GAGACAGCGT 
GGAGTGAAGT GTCCAAAGTG TTGTACCAGG AGAGGATGTC TCTGCAAATG CTGTATCTTC 
TGCTTTGAGA CCAAGTGCTT GTGTGGAAAA CGCAAAGTGA AGAAGAAGCC ATTCCCGAAG 
CCTGTGTTGT TCGTGGAACC AGAAGGTTAC GATGATACTG TGAACATCTT CGAATCTCGC 
AACTTGTACG GTTGGTGGCC AATGCTTACC GAGGAGTATC CACACGAGGA ACCTCAGAAT 
GCCAAGAAGA AGAATGATGA CGTTGGAAAG GATCCTAAAT GGATCATGGG TCTTGTTGAG 
ATGGACATGC TACTTCTCAC CAAACAAGAA GCCGATCAAG AGCCAGCTGG AAAGAAACGA 
TCCGAGCCGA ACCACAGCCC ATTCTTGGAA AAACCTGATC GGAAGTCATG GGCCAACTCG 
TGGTTGGTGT CCCGAATCAA GCCGTGCATC AAGTATTTCT GGCACTACTA TGGTCTTCAA 
ATTCTGCTCT GGCTCATCAT TATTGTTATT CTGATTCTCA CAATTTTCGT GTTGTTGCAC 
ACTTGGCCGA CAATTTTAGC CGAAATTATA AAGGCAATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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fer-1 cDNA ORF clone, Caenorhabditis elegans

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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