Dysf gene cDNA ORF clone, Rattus norvegicus(Norway rat)

mRNA	Protein	Name
XM_017592633.1	XP_017448122.1	dysferlin isoform X8
XM_017592634.1	XP_017448123.1	dysferlin isoform X9
XM_006236767.3	XP_006236829.1	dysferlin isoform X13
XM_006236768.3	XP_006236830.1	dysferlin isoform X14
XM_006236775.2	XP_006236837.1	dysferlin isoform X21
XM_017592635.1	XP_017448124.1	dysferlin isoform X23
NM_001107869.1	NP_001101339.1	dysferlin
XM_017592630.1	XP_017448119.1	dysferlin isoform X2
XM_017592631.1	XP_017448120.1	dysferlin isoform X3
XM_006236770.3	XP_006236832.1	dysferlin isoform X16
XM_006236773.3	XP_006236835.1	dysferlin isoform X19
XM_017592629.1	XP_017448118.1	dysferlin isoform X1
XM_006236758.3	XP_006236820.1	dysferlin isoform X4
XM_006236766.3	XP_006236828.1	dysferlin isoform X12
XM_006236771.3	XP_006236833.1	dysferlin isoform X17
XM_006236772.3	XP_006236834.1	dysferlin isoform X18
XM_017592632.1	XP_017448121.1	dysferlin isoform X5
XM_006236764.3	XP_006236826.1	dysferlin isoform X10
XM_006236769.3	XP_006236831.1	dysferlin isoform X15
XM_006236774.3	XP_006236836.1	dysferlin isoform X20
XM_008763068.2	XP_008761290.1	dysferlin isoform X22
XM_006236760.3	XP_006236822.1	dysferlin isoform X6
XM_006236761.3	XP_006236823.1	dysferlin isoform X7
XM_006236765.3	XP_006236827.1	dysferlin isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa60863	XM_017592635.1 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa47141	XM_008763068.2 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa60857	XM_017592629.1 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa60858	XM_017592630.1 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa60859	XM_017592631.1 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa60860	XM_017592632.1 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa60861	XM_017592633.1 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa60862	XM_017592634.1 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa12046	NM_001107869.1 Latest version!	Rattus norvegicus dysferlin (Dysf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27399	XM_006236758.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27401	XM_006236760.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27402	XM_006236761.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27405	XM_006236764.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27406	XM_006236765.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27407	XM_006236766.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27408	XM_006236767.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27409	XM_006236768.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27410	XM_006236769.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27411	XM_006236770.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27412	XM_006236771.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27413	XM_006236772.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27414	XM_006236773.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27415	XM_006236774.3 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa27416	XM_006236775.2 Latest version!	Rattus norvegicus dysferlin (Dysf), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

Dysf ( XM_017592635.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448124.1 Rattus norvegicus dysferlin (Dysf), transcript variant X23, mRNA.

Price & Availability↑

CloneID	ORa60863
Clone ID Related Accession (Same CDS sequence)	XM_017592635.1
Accession Version	XM_017592635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGCTGTGCGC TCCTGCCTTC 
TTCCTGCAGG GCCTACTCCA GACGACGGAG GACACAGACT TGCCTTACCC ACCACCTCAA 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGCTCTCCA GCGTACAGCC 
ATCGAGATCC TGGCCTGGGG CCTTCGGAAC ATAAAGAGCT ATCAGATGGC CAGCGTGTCT 
TCCCCCAGCC TCGTGGTGGA GTGTGGTGGG CAGACAGTGC AGTCCTGTGT CATCAGAAAC 
CTTCGGAAGA ACCCCAACTT TGACGTCTGT ACTCTGTTCA TGGAAGTGAT GTTACCCAGA 
GAGGACCTCT ACTGTCCCCC TATTGTGGTC AAGGTCATTG ATAATCGCCA GTTTGGCCGC 
AGGCCAGTTG TGGGTCAGTG CACCATTCGC TCCCTGGAGA ACTTCCTGTG TGACCCGTAC 
TCAGCAGAGA GTCCATCCCC GCAAGGTGGC CCAGATGACG TGAGCTTACT CAGCCCTGGG 
GAAGACGTGC TCATTGACAT TGATGACAAG GAGCCTCTCA TCCCTGTCCA GCTTGCAGAT 
GGGCTGTCAA GCTTGGGCCC CACTAACCTG ATGCCTTCTC CGTCCAGTCC TCATGAGGAG 
GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTATCG GGGAGAGGGA AAAGTGTGGT 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG GAGGCCTGTC CGACTTTTGT AATACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGACA CAGAAGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT 
CCCCTCCCAG AAGATCCAGC CATCCCTATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT 
CAGGGTCCCC AGGAATGCTT GGTCCGGATT TACATCATCC GAGCATTTGG CTTACAGCCC 
AAGGACCCCA ATGGCAAGTG CGACCCTTAC ATCAAGATCT CTATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCTTGTACC CTGGAGCCTG TATTTGGAAA GATGTTTGAA 
CTCACCTGCA CTCTACCTCT GGAGAAGGAC TTGAAGATCA CACTCTATGA CTATGACCTG 
CTCTCCAAGG ATGAGAAGAT TGGGGAGACA GTCATTGATC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG CCTTCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG 
AGAGATCAAC TCCGCCCTTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGAATACAC CATTCAGGAG 
ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCGG TGGAGGAACG CTTAGCCCTG 
CATGTCCTTC AACAACAAGG CTTGGTCCCT GAGCATGTGG AGTCTCGGCC TCTGTACAGT 
CCTCTGCAGC CAGATATTGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG 
GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTCG AAGGTTTTTT 
TCTGCGTTGT ATTATTTGGA ACACAAAAGA TGTGATCTTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448124.1
CDS	706..6390
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDELCAPAFFLQGLLQTTEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFSALYYLEHKRCDLG

Target ORF information:

RefSeq Version	XM_017592635.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592635.1

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGCTGTGCGC TCCTGCCTTC 
TTCCTGCAGG GCCTACTCCA GACGACGGAG GACACAGACT TGCCTTACCC ACCACCTCAA 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGCTCTCCA GCGTACAGCC 
ATCGAGATCC TGGCCTGGGG CCTTCGGAAC ATAAAGAGCT ATCAGATGGC CAGCGTGTCT 
TCCCCCAGCC TCGTGGTGGA GTGTGGTGGG CAGACAGTGC AGTCCTGTGT CATCAGAAAC 
CTTCGGAAGA ACCCCAACTT TGACGTCTGT ACTCTGTTCA TGGAAGTGAT GTTACCCAGA 
GAGGACCTCT ACTGTCCCCC TATTGTGGTC AAGGTCATTG ATAATCGCCA GTTTGGCCGC 
AGGCCAGTTG TGGGTCAGTG CACCATTCGC TCCCTGGAGA ACTTCCTGTG TGACCCGTAC 
TCAGCAGAGA GTCCATCCCC GCAAGGTGGC CCAGATGACG TGAGCTTACT CAGCCCTGGG 
GAAGACGTGC TCATTGACAT TGATGACAAG GAGCCTCTCA TCCCTGTCCA GCTTGCAGAT 
GGGCTGTCAA GCTTGGGCCC CACTAACCTG ATGCCTTCTC CGTCCAGTCC TCATGAGGAG 
GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTATCG GGGAGAGGGA AAAGTGTGGT 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG GAGGCCTGTC CGACTTTTGT AATACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGACA CAGAAGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT 
CCCCTCCCAG AAGATCCAGC CATCCCTATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT 
CAGGGTCCCC AGGAATGCTT GGTCCGGATT TACATCATCC GAGCATTTGG CTTACAGCCC 
AAGGACCCCA ATGGCAAGTG CGACCCTTAC ATCAAGATCT CTATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCTTGTACC CTGGAGCCTG TATTTGGAAA GATGTTTGAA 
CTCACCTGCA CTCTACCTCT GGAGAAGGAC TTGAAGATCA CACTCTATGA CTATGACCTG 
CTCTCCAAGG ATGAGAAGAT TGGGGAGACA GTCATTGATC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG CCTTCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG 
AGAGATCAAC TCCGCCCTTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGAATACAC CATTCAGGAG 
ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCGG TGGAGGAACG CTTAGCCCTG 
CATGTCCTTC AACAACAAGG CTTGGTCCCT GAGCATGTGG AGTCTCGGCC TCTGTACAGT 
CCTCTGCAGC CAGATATTGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG 
GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTCG AAGGTTTTTT 
TCTGCGTTGT ATTATTTGGA ACACAAAAGA TGTGATCTTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_008763068.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761290.1 Rattus norvegicus dysferlin (Dysf), transcript variant X22, mRNA.

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CloneID	ORa47141
Clone ID Related Accession (Same CDS sequence)	XM_008763068.2
Accession Version	XM_008763068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG TGGCTGACAC AGGAGGAGAG GAAGACACGG AAGACCAGGG CCTGACTGGA 
GATGAAGCTG AGCTCTTCCC AGATCCGAGT GTTGCTGTGG GCCCAGGGGG ACCAACGACG 
CCCAAGAAGC CACCTTCCCA TCCTCCTCCC CACTATCCAG GGGGGAAAAG GAAAAGAAGC 
TCTGCTCCAC CTAGAAAGCT GCTGTCAGAC AAGCCACAGG ACTTCCAGAT CAGAGTCCAG 
GTGATTGAGG GGCGTCAGCT GCCTGGGGTG AACATCAAGC CTGTGGTCAA GGTCACAGCT 
GCTGGGCAGA CCAAGCGGAC TCGAATCCAG AAGGGAAATA GCCCACTTTT CAATGAGACT 
CTTTTCTTCA ACGTATTTGA CTCTCCCTTG GAACTGTTTG ATGAGCCCAT CTTCATCACG 
GTGGTAGACT CACGCTCCCT CAGGACAGAT GCCCTCCTTG GCGAGTTCCG GATGGATGTG 
GGCACCGTGT ACAGAGAGCC CCGACATGCT TATCTCCGGA AGTGGCTGCT GCTCTCCGAT 
CCCGATGACT TCTCTGCAGG GGCCAGAGGC TACCTCAAAG CCAGCCTGTG TGTGCTGGGG 
CCTGGGGACG AAGCTCCCCT AGACAAGAAA GACCCTTCTG AAGACAAAGA GGACATTGAA 
GGCAACCTGC TTAGGCCCAC TGGTGTGGCC CTTCGTGGTG CCCATTTTTG CCTGAAGCTC 
TTCCGGGCTG AGGACTTACC ACAGATGGAC GATGCTGTGA TGGACAACGT CAAGCAAATC 
TTTGGCTTTG ATAGCAACAA GAAGAACCTG GTGGACCCCT TTGTGGAAGT CAGCTTTGCT 
GGGAAAATGC TTTGCAGCAA GATCCTGGAG AAGACTGCTA ACCCTCAGTG GAACCAGAAC 
ATCACATTGC CTGCGATGTT TCCCTCTATG TGTGAAAAAA TGAGGATTCG TGTCATGGAC 
TGGGACCGCC TCACTCACAA TGACACTGTG GCTACCACCT ACTTGGGTAT GTCGAAAATC 
TCTGCCACTG GAGGAGAAAT AGAAGAGGAG CCTGCAGGTG TACTCAAGAG TCCAGCCACT 
GACCTGGATG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT 
GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CGGAGCTCAA CACAGGAAAG 
GGAGAAGGTG TGGCATATAG GGGCAGGCTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAG 
CACAGTGAAC AGAAGGTGGA AGACCTTCCT GCTGATGACA TCTTGAGAGT GGAGAAGTAC 
CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGAAACAA GTTTGACACC 
ACCTGCTTGC CGTTGGCCTC TACCACCCAA TACAGCCGGG CAGTCTTTGA TGGATGCCAC 
TACTATTATT TGCCCTGGGG CAACGTGAAG CCTGTGGTAG TACTGTCCTC ATACTGGGAA 
GACATCAGCC ATCGAGTCGA GACTCAGAAC CAGCTCCTCA GGATCGCTGA CCGACTGGAA 
GCTAACCTGG AGCAAGTCCA CCTGGCTCTG AAGGCACAGT GTTCCTCTGA GGATGTGGAC 
GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC 
ATCCATGAAA TACCTTCTGC CACCCACCTT GATCAGTACC TGTTCCGCCT CCGCTCCCGT 
CACCTGAGCC AGATCAAAGA AGCTGCCCTG GCCTTGAAGC TTGGCCACAG TGAGCTCTCC 
ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC TGAGGAGCCC 
CAGAACAGCC TGCCAGATAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC 
CAACGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG 
AACTGTGGGA ATCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGCAT GCCTGGGCCC 
CGGATGCCGG TGCAGATCCG GATCAAGCTC TGGTTTGGGC TCTCAGTGGA TGAGAAGGAA 
TTCAATCAGT TTGCAGAGGG GAAGCTCTCT GTCTTTGCTG AAACGTATGA GAACCAGACA 
AAGCTGGCGC TGGTCGGGAA CTGGGGCACG ACGGGCCTCA CCTACCCCAA GTTTTCTGAC 
GTCACAGGCA AGATCAAACT ACCGAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCAGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTGCTCC ATGATGCTGA CGCCGGTCAT 
CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACTCGGC TACCTGGAGG CCAGTGGATC 
TATATGAGTG ACAACTACAC CGACGTGAAT GGGGAGAAGG TGCTTCCAAA GGACGATATT 
GAATGCCCGC TGGGTTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT 
GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCCAAACAC 
TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCACTG AAGAAGCACA GGCAGGCAGA GGCTGAGGGC 
GAGGGCTGGG AATACGCCTC TCTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GATGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACGGGGCCT 
GCAGCGGTGT TTGCCCTTGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAT 
TCCATGTCTG TGTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTGCGTTGC TACCTGTACC AGGCCCGGGA CCTGCCCGCC 
ATGGACAAAG ACTCCTTCTC TGATCCCTAT GCCATTGTCT CCTTCCTGCA TCAAAGCCAG 
AAGACGGTGG TGGAGAAGAA CACCCTGAAC CCCACCTGGG ATCAAACCCT CATCTTCTAT 
GAGATAGAGA TCTTTGGCGA GCCGGCCAGT GTCGCCGAGC GGCCACCCAG CATTGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGCGCT GACGAGTTCA TGGGCCGCTG TATTTGTCAG 
CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCAGGGG CAGCCAGCCT 
GCCGGAGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC 
ATCCCTGGCT TTGAGATGCA CGAAACTTCA AGGATTTTGG ATGAGACGGA GGACACAGAC 
TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCAGCTCTCC AGCGTACAGC CATCGAGATC CTGGCCTGGG GCCTTCGGAA CATAAAGAGC 
TATCAGATGG CCAGCGTGTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG 
CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGACGTCTG TACTCTGTTC 
ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCCC CTATTGTGGT CAAGGTCATT 
GATAATCGCC AGTTTGGCCG CAGGCCAGTT GTGGGTCAGT GCACCATTCG CTCCCTGGAG 
AACTTCCTGT GTGACCCGTA CTCAGCAGAG AGTCCATCCC CGCAAGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGCCCTGG GGAAGACGTG CTCATTGACA TTGATGACAA GGAGCCTCTC 
ATCCCTGTCC AGGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTATCGGG 
GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCCTGAA GGTCTATGAC 
ACACAGCTGG AGAATGTGGA GGCCTTTGGA GGCCTGTCCG ACTTTTGTAA TACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGACACA GAAGATCCAT CTGTCATCGG AGAATTTAAG 
GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCTATGCC CCCGAGACAA 
TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGGATTTA CATCATCCGA 
GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGCG ACCCTTACAT CAAGATCTCT 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGTACCCT GGAGCCTGTA 
TTTGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACTT GAAGATCACA 
CTCTATGACT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACAGT CATTGATCTG 
GAGAATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGCC TTCCACAGAC CTACTGTGTC 
TCTGGACCAA ATCAGTGGAG AGATCAACTC CGCCCTTCCC AGCTCCTCCA CCTCTTCTGC 
CAGCAGCACA GGGTCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG 
GAATACACCA TTCAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCGGTG 
GAGGAACGCT TAGCCCTGCA TGTCCTTCAA CAACAAGGCT TGGTCCCTGA GCATGTGGAG 
TCTCGGCCTC TGTACAGTCC TCTGCAGCCA GATATTGAGC AGGGGAAGCT ACAGATGTGG 
ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG 
AAAGCTCGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT 
GACCTCAGCC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA 
TTCGAAGAAC ACAAACAGAA GACAGATGTG CACTACCGTT CCCTGGGCGG GGAGGGCAAT 
TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCCG CTGAGCAAGT CTGTGCTGTT 
GCCAAGAAGG ATGCTTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG 
GTCTTCCAGA TTTGGGACAA TGACAAGTTC TCCTTTGATG ACTTTCTGGG CTCCCTGCAG 
CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA 
GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GCGAAGAAGA TCCTGGCGGG GAAGCTGGAA 
ATGACCTTGG AGATCGTTGC AGAAAGTGAA CATGAGGAGC GGCCTGCTGG CCAGGGTCGG 
GATGAACCCA ACATGAATCC AAAGCTAGAA GATCCCAGGC GCCCTGACAC CTCTTTCCTG 
TGGTTCACCT CACCATACAA GACCATGAAG TTCATCTTGT GGCGACGATT CCGGTGTGCC 
ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC 
TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTTA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761290.1
CDS	476..6280
Translation	MDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_008763068.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763068.2

ORF Insert Sequence:

ATGGACCTGG TGGCTGACAC AGGAGGAGAG GAAGACACGG AAGACCAGGG CCTGACTGGA 
GATGAAGCTG AGCTCTTCCC AGATCCGAGT GTTGCTGTGG GCCCAGGGGG ACCAACGACG 
CCCAAGAAGC CACCTTCCCA TCCTCCTCCC CACTATCCAG GGGGGAAAAG GAAAAGAAGC 
TCTGCTCCAC CTAGAAAGCT GCTGTCAGAC AAGCCACAGG ACTTCCAGAT CAGAGTCCAG 
GTGATTGAGG GGCGTCAGCT GCCTGGGGTG AACATCAAGC CTGTGGTCAA GGTCACAGCT 
GCTGGGCAGA CCAAGCGGAC TCGAATCCAG AAGGGAAATA GCCCACTTTT CAATGAGACT 
CTTTTCTTCA ACGTATTTGA CTCTCCCTTG GAACTGTTTG ATGAGCCCAT CTTCATCACG 
GTGGTAGACT CACGCTCCCT CAGGACAGAT GCCCTCCTTG GCGAGTTCCG GATGGATGTG 
GGCACCGTGT ACAGAGAGCC CCGACATGCT TATCTCCGGA AGTGGCTGCT GCTCTCCGAT 
CCCGATGACT TCTCTGCAGG GGCCAGAGGC TACCTCAAAG CCAGCCTGTG TGTGCTGGGG 
CCTGGGGACG AAGCTCCCCT AGACAAGAAA GACCCTTCTG AAGACAAAGA GGACATTGAA 
GGCAACCTGC TTAGGCCCAC TGGTGTGGCC CTTCGTGGTG CCCATTTTTG CCTGAAGCTC 
TTCCGGGCTG AGGACTTACC ACAGATGGAC GATGCTGTGA TGGACAACGT CAAGCAAATC 
TTTGGCTTTG ATAGCAACAA GAAGAACCTG GTGGACCCCT TTGTGGAAGT CAGCTTTGCT 
GGGAAAATGC TTTGCAGCAA GATCCTGGAG AAGACTGCTA ACCCTCAGTG GAACCAGAAC 
ATCACATTGC CTGCGATGTT TCCCTCTATG TGTGAAAAAA TGAGGATTCG TGTCATGGAC 
TGGGACCGCC TCACTCACAA TGACACTGTG GCTACCACCT ACTTGGGTAT GTCGAAAATC 
TCTGCCACTG GAGGAGAAAT AGAAGAGGAG CCTGCAGGTG TACTCAAGAG TCCAGCCACT 
GACCTGGATG ACAACCTGGG CTTCCTCCCC ACTTTTGGGC CCTGCTATGT GAACCTCTAT 
GGCAGTCCCC GAGAGTTCAC GGGCTTCCCA GATCCTTATG CGGAGCTCAA CACAGGAAAG 
GGAGAAGGTG TGGCATATAG GGGCAGGCTT CTGTTGTCCC TGGAGACCAA ACTGGTGGAG 
CACAGTGAAC AGAAGGTGGA AGACCTTCCT GCTGATGACA TCTTGAGAGT GGAGAAGTAC 
CTGCGGAGGC GCAAATATTC CCTCTTTGCT GCCTTCTACT CAGCCACAAT GCTGCAGGAT 
GTGGATGATG CCATCCAGTT CGAGGTCAGC ATTGGGAACT ACGGAAACAA GTTTGACACC 
ACCTGCTTGC CGTTGGCCTC TACCACCCAA TACAGCCGGG CAGTCTTTGA TGGATGCCAC 
TACTATTATT TGCCCTGGGG CAACGTGAAG CCTGTGGTAG TACTGTCCTC ATACTGGGAA 
GACATCAGCC ATCGAGTCGA GACTCAGAAC CAGCTCCTCA GGATCGCTGA CCGACTGGAA 
GCTAACCTGG AGCAAGTCCA CCTGGCTCTG AAGGCACAGT GTTCCTCTGA GGATGTGGAC 
GCCCTGGTGG CTCAGTTGAC AGATGAGCTC CTTGCAGACT GCAGCCAGCC CCTGTGTGAC 
ATCCATGAAA TACCTTCTGC CACCCACCTT GATCAGTACC TGTTCCGCCT CCGCTCCCGT 
CACCTGAGCC AGATCAAAGA AGCTGCCCTG GCCTTGAAGC TTGGCCACAG TGAGCTCTCC 
ACTGCCCTGG AGCAGGCCGA GGACTGGCTT CTGCATCTCC GTGCCCTGGC TGAGGAGCCC 
CAGAACAGCC TGCCAGATAT CATCATCTGG ATGCTGCAGG GTGACAAGCG TGTGGCCTAC 
CAACGGGTGC CTGCCCATGA AGTCCTCTTC TCCCGGCGGG GCCCCAGCTA CTGCGGCAGG 
AACTGTGGGA ATCTGCAGAC CATCTTTCTG AAATATCCTA TGGAAGGCAT GCCTGGGCCC 
CGGATGCCGG TGCAGATCCG GATCAAGCTC TGGTTTGGGC TCTCAGTGGA TGAGAAGGAA 
TTCAATCAGT TTGCAGAGGG GAAGCTCTCT GTCTTTGCTG AAACGTATGA GAACCAGACA 
AAGCTGGCGC TGGTCGGGAA CTGGGGCACG ACGGGCCTCA CCTACCCCAA GTTTTCTGAC 
GTCACAGGCA AGATCAAACT ACCGAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGGCCTGG 
GCAGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTGCTCC ATGATGCTGA CGCCGGTCAT 
CTGAGCTTTG TGGAGGAGGT GTTTGAGAAC CAGACTCGGC TACCTGGAGG CCAGTGGATC 
TATATGAGTG ACAACTACAC CGACGTGAAT GGGGAGAAGG TGCTTCCAAA GGACGATATT 
GAATGCCCGC TGGGTTGGAA GTGGGAAGAT GAAGAATGGT CCACAGACCT CAATCGGGCT 
GTTGATGAAC AAGGCTGGGA GTACAGCATC ACCATACCCC CAGACCGAAA GCCCAAACAC 
TGGGTCCCTG TTGAGAAGAT GTATTACACT CACAGGCGTC GACGCTGGGT CCGACTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCACTG AAGAAGCACA GGCAGGCAGA GGCTGAGGGC 
GAGGGCTGGG AATACGCCTC TCTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GATGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CACTGGAGAA GACGGGGCCT 
GCAGCGGTGT TTGCCCTTGA GGGGGCCCTG GGCGGCATGG TGGATGACAA GAGCGAAGAT 
TCCATGTCTG TGTCTACCCT GAGCTTTGGC GTTAACAGAC CCACAATCTC CTGCATCTTT 
GACTATGGGA ACCGCTACCA TCTGCGTTGC TACCTGTACC AGGCCCGGGA CCTGCCCGCC 
ATGGACAAAG ACTCCTTCTC TGATCCCTAT GCCATTGTCT CCTTCCTGCA TCAAAGCCAG 
AAGACGGTGG TGGAGAAGAA CACCCTGAAC CCCACCTGGG ATCAAACCCT CATCTTCTAT 
GAGATAGAGA TCTTTGGCGA GCCGGCCAGT GTCGCCGAGC GGCCACCCAG CATTGTGGTG 
GAGCTGTATG ACCATGACAC CTATGGCGCT GACGAGTTCA TGGGCCGCTG TATTTGTCAG 
CCAAGCCTGG AACGGATGCC CCGCCTGGCC TGGTTCCCAC TGACCAGGGG CAGCCAGCCT 
GCCGGAGAGC TGCTGGCAGC GTTCGAGCTT ATCCAGAGAG AGAAGCCAGC CATCCATCAC 
ATCCCTGGCT TTGAGATGCA CGAAACTTCA AGGATTTTGG ATGAGACGGA GGACACAGAC 
TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCAGCTCTCC AGCGTACAGC CATCGAGATC CTGGCCTGGG GCCTTCGGAA CATAAAGAGC 
TATCAGATGG CCAGCGTGTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG 
CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGACGTCTG TACTCTGTTC 
ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCCC CTATTGTGGT CAAGGTCATT 
GATAATCGCC AGTTTGGCCG CAGGCCAGTT GTGGGTCAGT GCACCATTCG CTCCCTGGAG 
AACTTCCTGT GTGACCCGTA CTCAGCAGAG AGTCCATCCC CGCAAGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGCCCTGG GGAAGACGTG CTCATTGACA TTGATGACAA GGAGCCTCTC 
ATCCCTGTCC AGGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTATCGGG 
GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCCTGAA GGTCTATGAC 
ACACAGCTGG AGAATGTGGA GGCCTTTGGA GGCCTGTCCG ACTTTTGTAA TACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGACACA GAAGATCCAT CTGTCATCGG AGAATTTAAG 
GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCTATGCC CCCGAGACAA 
TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGGATTTA CATCATCCGA 
GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGCG ACCCTTACAT CAAGATCTCT 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGTACCCT GGAGCCTGTA 
TTTGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACTT GAAGATCACA 
CTCTATGACT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACAGT CATTGATCTG 
GAGAATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGCC TTCCACAGAC CTACTGTGTC 
TCTGGACCAA ATCAGTGGAG AGATCAACTC CGCCCTTCCC AGCTCCTCCA CCTCTTCTGC 
CAGCAGCACA GGGTCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG 
GAATACACCA TTCAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCGGTG 
GAGGAACGCT TAGCCCTGCA TGTCCTTCAA CAACAAGGCT TGGTCCCTGA GCATGTGGAG 
TCTCGGCCTC TGTACAGTCC TCTGCAGCCA GATATTGAGC AGGGGAAGCT ACAGATGTGG 
ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG 
AAAGCTCGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT 
GACCTCAGCC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA 
TTCGAAGAAC ACAAACAGAA GACAGATGTG CACTACCGTT CCCTGGGCGG GGAGGGCAAT 
TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCCG CTGAGCAAGT CTGTGCTGTT 
GCCAAGAAGG ATGCTTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG 
GTCTTCCAGA TTTGGGACAA TGACAAGTTC TCCTTTGATG ACTTTCTGGG CTCCCTGCAG 
CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA 
GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GCGAAGAAGA TCCTGGCGGG GAAGCTGGAA 
ATGACCTTGG AGATCGTTGC AGAAAGTGAA CATGAGGAGC GGCCTGCTGG CCAGGGTCGG 
GATGAACCCA ACATGAATCC AAAGCTAGAA GATCCCAGGC GCCCTGACAC CTCTTTCCTG 
TGGTTCACCT CACCATACAA GACCATGAAG TTCATCTTGT GGCGACGATT CCGGTGTGCC 
ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC 
TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTTA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_017592629.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448118.1 Rattus norvegicus dysferlin (Dysf), transcript variant X1, mRNA.

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CloneID	ORa60857
Clone ID Related Accession (Same CDS sequence)	XM_017592629.1
Accession Version	XM_017592629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGCTGTGCGC TCCTGCCTTC 
TTCCTGCAGG GCCTACTCCA GACGACGGAG GACACAGACT TGCCTTACCC ACCACCTCAA 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGCTCTCCA GCGTACAGCC 
ATCGAGATCC TGGCCTGGGG CCTTCGGAAC ATAAAGAGCT ATCAGATGGC CAGCGTGTCT 
TCCCCCAGCC TCGTGGTGGA GTGTGGTGGG CAGACAGTGC AGTCCTGTGT CATCAGAAAC 
CTTCGGAAGA ACCCCAACTT TGACGTCTGT ACTCTGTTCA TGGAAGTGAT GTTACCCAGA 
GAGGACCTCT ACTGTCCCCC TATTGTGGTC AAGGTCATTG ATAATCGCCA GTTTGGCCGC 
AGGCCAGTTG TGGGTCAGTG CACCATTCGC TCCCTGGAGA ACTTCCTGTG TGACCCGTAC 
TCAGCAGAGA GTCCATCCCC GCAAGGTGGC CCAGATGACG TGAGCTTACT CAGCCCTGGG 
GAAGACGTGC TCATTGACAT TGATGACAAG GAGCCTCTCA TCCCTGTCCA GCTTGCAGAT 
GGGCTGTCAA GCTTGGGCCC CACTAACCTG ATGCCTTCTC CGTCCAGTCC TCATGAGGAG 
GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTATCG GGGAGAGGGA AAAGTGTGGT 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG GAGGCCTGTC CGACTTTTGT AATACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGACA CAGAAGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT 
CCCCTCCCAG AAGATCCAGC CATCCCTATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT 
CAGGGTCCCC AGGAATGCTT GGTCCGGATT TACATCATCC GAGCATTTGG CTTACAGCCC 
AAGGACCCCA ATGGCAAGTG CGACCCTTAC ATCAAGATCT CTATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCTTGTACC CTGGAGCCTG TATTTGGAAA GATGTTTGAA 
CTCACCTGCA CTCTACCTCT GGAGAAGGAC TTGAAGATCA CACTCTATGA CTATGACCTG 
CTCTCCAAGG ATGAGAAGAT TGGGGAGACA GTCATTGATC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG CCTTCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG 
AGAGATCAAC TCCGCCCTTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGAATACAC CATTCAGGAG 
ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCGG TGGAGGAACG CTTAGCCCTG 
CATGTCCTTC AACAACAAGG CTTGGTCCCT GAGCATGTGG AGTCTCGGCC TCTGTACAGT 
CCTCTGCAGC CAGATATTGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG 
GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTCG AAGGTTTTTT 
CTGCGTTGTA TTATTTGGAA CACAAAAGAT GTGATCTTGG ATGACCTCAG CCTCACGGGG 
GAGAAGATGA GTGACATCTA TGTGAAAGGC TGGATGGTGG GATTCGAAGA ACACAAACAG 
AAGACAGATG TGCACTACCG TTCCCTGGGC GGGGAGGGCA ATTTCAACTG GAGGTTCGTG 
TTTCCCTTCG ACTATCTGCC CGCTGAGCAA GTCTGTGCTG TTGCCAAGAA GGATGCTTTC 
TGGAGACTCG ACAAGACAGA GAGCAAGATC CCAGCACGTG TGGTCTTCCA GATTTGGGAC 
AATGACAAGT TCTCCTTTGA TGACTTTCTG GGCTCCCTGC AGCTGGATCT CAACCGAATG 
CCCAAGCCAG CCAAGACGGC TGAGAAGTGC TCCTTGGACC AGCTGGATGA CACCTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAGACAGTGA AAGGATGGTG GCCTTGTGTG 
ACAGAGGAGG GCGCGAAGAA GATCCTGGCG GGGAAGCTGG AAATGACCTT GGAGATCGTT 
GCAGAAAGTG AACATGAGGA GCGGCCTGCT GGCCAGGGTC GGGATGAACC CAACATGAAT 
CCAAAGCTAG AAGATCCCAG GCGCCCTGAC ACCTCTTTCC TGTGGTTCAC CTCACCATAC 
AAGACCATGA AGTTCATCTT GTGGCGACGA TTCCGGTGTG CCATCATCCT CTTCATCATC 
CTCTTCATCC TCCTGCTCTT CCTGGGTGTC TTTGTCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT TAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448118.1
CDS	703..7149
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDELCAPAFFLQGLLQTTEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_017592629.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592629.1

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGCTGTGCGC TCCTGCCTTC 
TTCCTGCAGG GCCTACTCCA GACGACGGAG GACACAGACT TGCCTTACCC ACCACCTCAA 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGCTCTCCA GCGTACAGCC 
ATCGAGATCC TGGCCTGGGG CCTTCGGAAC ATAAAGAGCT ATCAGATGGC CAGCGTGTCT 
TCCCCCAGCC TCGTGGTGGA GTGTGGTGGG CAGACAGTGC AGTCCTGTGT CATCAGAAAC 
CTTCGGAAGA ACCCCAACTT TGACGTCTGT ACTCTGTTCA TGGAAGTGAT GTTACCCAGA 
GAGGACCTCT ACTGTCCCCC TATTGTGGTC AAGGTCATTG ATAATCGCCA GTTTGGCCGC 
AGGCCAGTTG TGGGTCAGTG CACCATTCGC TCCCTGGAGA ACTTCCTGTG TGACCCGTAC 
TCAGCAGAGA GTCCATCCCC GCAAGGTGGC CCAGATGACG TGAGCTTACT CAGCCCTGGG 
GAAGACGTGC TCATTGACAT TGATGACAAG GAGCCTCTCA TCCCTGTCCA GCTTGCAGAT 
GGGCTGTCAA GCTTGGGCCC CACTAACCTG ATGCCTTCTC CGTCCAGTCC TCATGAGGAG 
GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTATCG GGGAGAGGGA AAAGTGTGGT 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG GAGGCCTGTC CGACTTTTGT AATACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGACA CAGAAGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT 
CCCCTCCCAG AAGATCCAGC CATCCCTATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT 
CAGGGTCCCC AGGAATGCTT GGTCCGGATT TACATCATCC GAGCATTTGG CTTACAGCCC 
AAGGACCCCA ATGGCAAGTG CGACCCTTAC ATCAAGATCT CTATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCTTGTACC CTGGAGCCTG TATTTGGAAA GATGTTTGAA 
CTCACCTGCA CTCTACCTCT GGAGAAGGAC TTGAAGATCA CACTCTATGA CTATGACCTG 
CTCTCCAAGG ATGAGAAGAT TGGGGAGACA GTCATTGATC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG CCTTCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG 
AGAGATCAAC TCCGCCCTTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGAATACAC CATTCAGGAG 
ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCGG TGGAGGAACG CTTAGCCCTG 
CATGTCCTTC AACAACAAGG CTTGGTCCCT GAGCATGTGG AGTCTCGGCC TCTGTACAGT 
CCTCTGCAGC CAGATATTGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG 
GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTCG AAGGTTTTTT 
CTGCGTTGTA TTATTTGGAA CACAAAAGAT GTGATCTTGG ATGACCTCAG CCTCACGGGG 
GAGAAGATGA GTGACATCTA TGTGAAAGGC TGGATGGTGG GATTCGAAGA ACACAAACAG 
AAGACAGATG TGCACTACCG TTCCCTGGGC GGGGAGGGCA ATTTCAACTG GAGGTTCGTG 
TTTCCCTTCG ACTATCTGCC CGCTGAGCAA GTCTGTGCTG TTGCCAAGAA GGATGCTTTC 
TGGAGACTCG ACAAGACAGA GAGCAAGATC CCAGCACGTG TGGTCTTCCA GATTTGGGAC 
AATGACAAGT TCTCCTTTGA TGACTTTCTG GGCTCCCTGC AGCTGGATCT CAACCGAATG 
CCCAAGCCAG CCAAGACGGC TGAGAAGTGC TCCTTGGACC AGCTGGATGA CACCTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAGACAGTGA AAGGATGGTG GCCTTGTGTG 
ACAGAGGAGG GCGCGAAGAA GATCCTGGCG GGGAAGCTGG AAATGACCTT GGAGATCGTT 
GCAGAAAGTG AACATGAGGA GCGGCCTGCT GGCCAGGGTC GGGATGAACC CAACATGAAT 
CCAAAGCTAG AAGATCCCAG GCGCCCTGAC ACCTCTTTCC TGTGGTTCAC CTCACCATAC 
AAGACCATGA AGTTCATCTT GTGGCGACGA TTCCGGTGTG CCATCATCCT CTTCATCATC 
CTCTTCATCC TCCTGCTCTT CCTGGGTGTC TTTGTCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT TAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_017592630.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448119.1 Rattus norvegicus dysferlin (Dysf), transcript variant X2, mRNA.

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CloneID	ORa60858
Clone ID Related Accession (Same CDS sequence)	XM_017592630.1
Accession Version	XM_017592630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ATTCTGGGAA GAAGTGGCCA 
GTCCCCACGG ACACAGGAGG AGAGGAAGAC ACGGAAGACC AGGGCCTGAC TGGAGATGAA 
GCTGAGCTCT TCCCAGATCC GAGTGTTGCT GTGGGCCCAG GGGGACCAAC GACGCCCAAG 
AAGCCACCTT CCCATCCTCC TCCCCACTAT CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT 
CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATT 
GAGGGGCGTC AGCTGCCTGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG 
CAGACCAAGC GGACTCGAAT CCAGAAGGGA AATAGCCCAC TTTTCAATGA GACTCTTTTC 
TTCAACGTAT TTGACTCTCC CTTGGAACTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCACGCT CCCTCAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC 
GTGTACAGAG AGCCCCGACA TGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGATCCCGAT 
GACTTCTCTG CAGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG 
GACGAAGCTC CCCTAGACAA GAAAGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC 
CTGCTTAGGC CCACTGGTGT GGCCCTTCGT GGTGCCCATT TTTGCCTGAA GCTCTTCCGG 
GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA AATCTTTGGC 
TTTGATAGCA ACAAGAAGAA CCTGGTGGAC CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA 
ATGCTTTGCA GCAAGATCCT GGAGAAGACT GCTAACCCTC AGTGGAACCA GAACATCACA 
TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACTC ACAATGACAC TGTGGCTACC ACCTACTTGG GTATGTCGAA AATCTCTGCC 
ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCAGC CACTGACCTG 
GATGACAACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ATGTGAACCT CTATGGCAGT 
CCCCGAGAGT TCACGGGCTT CCCAGATCCT TATGCGGAGC TCAACACAGG AAAGGGAGAA 
GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG TCCCTGGAGA CCAAACTGGT GGAGCACAGT 
GAACAGAAGG TGGAAGACCT TCCTGCTGAT GACATCTTGA GAGTGGAGAA GTACCTGCGG 
AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC TACTCAGCCA CAATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTCGAGGT CAGCATTGGG AACTACGGAA ACAAGTTTGA CACCACCTGC 
TTGCCGTTGG CCTCTACCAC CCAATACAGC CGGGCAGTCT TTGATGGATG CCACTACTAT 
TATTTGCCCT GGGGCAACGT GAAGCCTGTG GTAGTACTGT CCTCATACTG GGAAGACATC 
AGCCATCGAG TCGAGACTCA GAACCAGCTC CTCAGGATCG CTGACCGACT GGAAGCTAAC 
CTGGAGCAAG TCCACCTGGC TCTGAAGGCA CAGTGTTCCT CTGAGGATGT GGACGCCCTG 
GTGGCTCAGT TGACAGATGA GCTCCTTGCA GACTGCAGCC AGCCCCTGTG TGACATCCAT 
GAAATACCTT CTGCCACCCA CCTTGATCAG TACCTGTTCC GCCTCCGCTC CCGTCACCTG 
AGCCAGATCA AAGAAGCTGC CCTGGCCTTG AAGCTTGGCC ACAGTGAGCT CTCCACTGCC 
CTGGAGCAGG CCGAGGACTG GCTTCTGCAT CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC 
AGCCTGCCAG ATATCATCAT CTGGATGCTG CAGGGTGACA AGCGTGTGGC CTACCAACGG 
GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG CGGGGCCCCA GCTACTGCGG CAGGAACTGT 
GGGAATCTGC AGACCATCTT TCTGAAATAT CCTATGGAAG GCATGCCTGG GCCCCGGATG 
CCGGTGCAGA TCCGGATCAA GCTCTGGTTT GGGCTCTCAG TGGATGAGAA GGAATTCAAT 
CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT GCTGAAACGT ATGAGAACCA GACAAAGCTG 
GCGCTGGTCG GGAACTGGGG CACGACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACA 
GGCAAGATCA AACTACCGAA GGATAGCTTC CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA 
GACTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGATG CTGACGCCGG TCATCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACT CGGCTACCTG GAGGCCAGTG GATCTATATG 
AGTGACAACT ACACCGACGT GAATGGGGAG AAGGTGCTTC CAAAGGACGA TATTGAATGC 
CCGCTGGGTT GGAAGTGGGA AGATGAAGAA TGGTCCACAG ACCTCAATCG GGCTGTTGAT 
GAACAAGGCT GGGAGTACAG CATCACCATA CCCCCAGACC GAAAGCCCAA ACACTGGGTC 
CCTGTTGAGA AGATGTATTA CACTCACAGG CGTCGACGCT GGGTCCGACT GCGCAGGAGG 
GACCTCAGCC AGATGGAGGC ACTGAAGAAG CACAGGCAGG CAGAGGCTGA GGGCGAGGGC 
TGGGAATACG CCTCTCTCTT TGGCTGGAAG TTCCACCTGG AGTACCGCAA GACAGATGCC 
TTTCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCG 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC ATGGTGGATG ACAAGAGCGA AGATTCCATG 
TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC AGACCCACAA TCTCCTGCAT CTTTGACTAT 
GGGAACCGCT ACCATCTGCG TTGCTACCTG TACCAGGCCC GGGACCTGCC CGCCATGGAC 
AAAGACTCCT TCTCTGATCC CTATGCCATT GTCTCCTTCC TGCATCAAAG CCAGAAGACG 
GTGGTGGAGA AGAACACCCT GAACCCCACC TGGGATCAAA CCCTCATCTT CTATGAGATA 
GAGATCTTTG GCGAGCCGGC CAGTGTCGCC GAGCGGCCAC CCAGCATTGT GGTGGAGCTG 
TATGACCATG ACACCTATGG CGCTGACGAG TTCATGGGCC GCTGTATTTG TCAGCCAAGC 
CTGGAACGGA TGCCCCGCCT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCTGCCGGA 
GAGCTGCTGG CAGCGTTCGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA TCACATCCCT 
GGCTTTGAGA TGCACGAAAC TTCAAGGATT TTGGATGAGC TGTGCGCTCC TGCCTTCTTC 
CTGCAGGGCC TACTCCAGAC GACGGAGGAC ACAGACTTGC CTTACCCACC ACCTCAAAGG 
GAGGCCAACA TCTACATGGT TCCCCAGAAC ATCAAGCCAG CTCTCCAGCG TACAGCCATC 
GAGATCCTGG CCTGGGGCCT TCGGAACATA AAGAGCTATC AGATGGCCAG CGTGTCTTCC 
CCCAGCCTCG TGGTGGAGTG TGGTGGGCAG ACAGTGCAGT CCTGTGTCAT CAGAAACCTT 
CGGAAGAACC CCAACTTTGA CGTCTGTACT CTGTTCATGG AAGTGATGTT ACCCAGAGAG 
GACCTCTACT GTCCCCCTAT TGTGGTCAAG GTCATTGATA ATCGCCAGTT TGGCCGCAGG 
CCAGTTGTGG GTCAGTGCAC CATTCGCTCC CTGGAGAACT TCCTGTGTGA CCCGTACTCA 
GCAGAGAGTC CATCCCCGCA AGGTGGCCCA GATGACGTGA GCTTACTCAG CCCTGGGGAA 
GACGTGCTCA TTGACATTGA TGACAAGGAG CCTCTCATCC CTGTCCAGCT TGCAGATGGG 
CTGTCAAGCT TGGGCCCCAC TAACCTGATG CCTTCTCCGT CCAGTCCTCA TGAGGAGGAG 
TTCATTGACT GGTGGAGCAA ATTCTTTGCT TCTATCGGGG AGAGGGAAAA GTGTGGTTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGGAG GCCTGTCCGA CTTTTGTAAT ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGACACAG AAGATCCATC TGTCATCGGA GAATTTAAGG GTCTCTTCAA AATTTATCCC 
CTCCCAGAAG ATCCAGCCAT CCCTATGCCC CCGAGACAAT TCCATCAGCT GGCTGCTCAG 
GGTCCCCAGG AATGCTTGGT CCGGATTTAC ATCATCCGAG CATTTGGCTT ACAGCCCAAG 
GACCCCAATG GCAAGTGCGA CCCTTACATC AAGATCTCTA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC TTGTACCCTG GAGCCTGTAT TTGGAAAGAT GTTTGAACTC 
ACCTGCACTC TACCTCTGGA GAAGGACTTG AAGATCACAC TCTATGACTA TGACCTGCTC 
TCCAAGGATG AGAAGATTGG GGAGACAGTC ATTGATCTGG AGAATAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGCCT TCCACAGACC TACTGTGTCT CTGGACCAAA TCAGTGGAGA 
GATCAACTCC GCCCTTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCACAG GGTCAAGGCC 
CCCGTGTACC GGACAGACCG AGTGACGTTT CAGGATAAGG AATACACCAT TCAGGAGATA 
GAGGCTGGCA GACTCCCAAA CCCACACCTG GGCCCGGTGG AGGAACGCTT AGCCCTGCAT 
GTCCTTCAAC AACAAGGCTT GGTCCCTGAG CATGTGGAGT CTCGGCCTCT GTACAGTCCT 
CTGCAGCCAG ATATTGAGCA GGGGAAGCTA CAGATGTGGA TTGACATATT TCCAAAGGTG 
CTGGGCCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA AAGCTCGAAG GTTTTTTCTG 
CGTTGTATTA TTTGGAACAC AAAAGATGTG ATCTTGGATG ACCTCAGCCT CACGGGGGAG 
AAGATGAGTG ACATCTATGT GAAAGGCTGG ATGGTGGGAT TCGAAGAACA CAAACAGAAG 
ACAGATGTGC ACTACCGTTC CCTGGGCGGG GAGGGCAATT TCAACTGGAG GTTCGTGTTT 
CCCTTCGACT ATCTGCCCGC TGAGCAAGTC TGTGCTGTTG CCAAGAAGGA TGCTTTCTGG 
AGACTCGACA AGACAGAGAG CAAGATCCCA GCACGTGTGG TCTTCCAGAT TTGGGACAAT 
GACAAGTTCT CCTTTGATGA CTTTCTGGGC TCCCTGCAGC TGGATCTCAA CCGAATGCCC 
AAGCCAGCCA AGACGGCTGA GAAGTGCTCC TTGGACCAGC TGGATGACAC CTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAG ACAGTGAAAG GATGGTGGCC TTGTGTGACA 
GAGGAGGGCG CGAAGAAGAT CCTGGCGGGG AAGCTGGAAA TGACCTTGGA GATCGTTGCA 
GAAAGTGAAC ATGAGGAGCG GCCTGCTGGC CAGGGTCGGG ATGAACCCAA CATGAATCCA 
AAGCTAGAAG ATCCCAGGCG CCCTGACACC TCTTTCCTGT GGTTCACCTC ACCATACAAG 
ACCATGAAGT TCATCTTGTG GCGACGATTC CGGTGTGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTCC TGCTCTTCCT GGGTGTCTTT GTCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448119.1
CDS	407..6850
Translation	MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDELCAPAFFLQGLLQTTEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_017592630.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592630.1

ORF Insert Sequence:

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ATTCTGGGAA GAAGTGGCCA 
GTCCCCACGG ACACAGGAGG AGAGGAAGAC ACGGAAGACC AGGGCCTGAC TGGAGATGAA 
GCTGAGCTCT TCCCAGATCC GAGTGTTGCT GTGGGCCCAG GGGGACCAAC GACGCCCAAG 
AAGCCACCTT CCCATCCTCC TCCCCACTAT CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT 
CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATT 
GAGGGGCGTC AGCTGCCTGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG 
CAGACCAAGC GGACTCGAAT CCAGAAGGGA AATAGCCCAC TTTTCAATGA GACTCTTTTC 
TTCAACGTAT TTGACTCTCC CTTGGAACTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCACGCT CCCTCAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC 
GTGTACAGAG AGCCCCGACA TGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGATCCCGAT 
GACTTCTCTG CAGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG 
GACGAAGCTC CCCTAGACAA GAAAGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC 
CTGCTTAGGC CCACTGGTGT GGCCCTTCGT GGTGCCCATT TTTGCCTGAA GCTCTTCCGG 
GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA AATCTTTGGC 
TTTGATAGCA ACAAGAAGAA CCTGGTGGAC CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA 
ATGCTTTGCA GCAAGATCCT GGAGAAGACT GCTAACCCTC AGTGGAACCA GAACATCACA 
TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACTC ACAATGACAC TGTGGCTACC ACCTACTTGG GTATGTCGAA AATCTCTGCC 
ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCAGC CACTGACCTG 
GATGACAACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ATGTGAACCT CTATGGCAGT 
CCCCGAGAGT TCACGGGCTT CCCAGATCCT TATGCGGAGC TCAACACAGG AAAGGGAGAA 
GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG TCCCTGGAGA CCAAACTGGT GGAGCACAGT 
GAACAGAAGG TGGAAGACCT TCCTGCTGAT GACATCTTGA GAGTGGAGAA GTACCTGCGG 
AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC TACTCAGCCA CAATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTCGAGGT CAGCATTGGG AACTACGGAA ACAAGTTTGA CACCACCTGC 
TTGCCGTTGG CCTCTACCAC CCAATACAGC CGGGCAGTCT TTGATGGATG CCACTACTAT 
TATTTGCCCT GGGGCAACGT GAAGCCTGTG GTAGTACTGT CCTCATACTG GGAAGACATC 
AGCCATCGAG TCGAGACTCA GAACCAGCTC CTCAGGATCG CTGACCGACT GGAAGCTAAC 
CTGGAGCAAG TCCACCTGGC TCTGAAGGCA CAGTGTTCCT CTGAGGATGT GGACGCCCTG 
GTGGCTCAGT TGACAGATGA GCTCCTTGCA GACTGCAGCC AGCCCCTGTG TGACATCCAT 
GAAATACCTT CTGCCACCCA CCTTGATCAG TACCTGTTCC GCCTCCGCTC CCGTCACCTG 
AGCCAGATCA AAGAAGCTGC CCTGGCCTTG AAGCTTGGCC ACAGTGAGCT CTCCACTGCC 
CTGGAGCAGG CCGAGGACTG GCTTCTGCAT CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC 
AGCCTGCCAG ATATCATCAT CTGGATGCTG CAGGGTGACA AGCGTGTGGC CTACCAACGG 
GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG CGGGGCCCCA GCTACTGCGG CAGGAACTGT 
GGGAATCTGC AGACCATCTT TCTGAAATAT CCTATGGAAG GCATGCCTGG GCCCCGGATG 
CCGGTGCAGA TCCGGATCAA GCTCTGGTTT GGGCTCTCAG TGGATGAGAA GGAATTCAAT 
CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT GCTGAAACGT ATGAGAACCA GACAAAGCTG 
GCGCTGGTCG GGAACTGGGG CACGACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACA 
GGCAAGATCA AACTACCGAA GGATAGCTTC CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA 
GACTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGATG CTGACGCCGG TCATCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACT CGGCTACCTG GAGGCCAGTG GATCTATATG 
AGTGACAACT ACACCGACGT GAATGGGGAG AAGGTGCTTC CAAAGGACGA TATTGAATGC 
CCGCTGGGTT GGAAGTGGGA AGATGAAGAA TGGTCCACAG ACCTCAATCG GGCTGTTGAT 
GAACAAGGCT GGGAGTACAG CATCACCATA CCCCCAGACC GAAAGCCCAA ACACTGGGTC 
CCTGTTGAGA AGATGTATTA CACTCACAGG CGTCGACGCT GGGTCCGACT GCGCAGGAGG 
GACCTCAGCC AGATGGAGGC ACTGAAGAAG CACAGGCAGG CAGAGGCTGA GGGCGAGGGC 
TGGGAATACG CCTCTCTCTT TGGCTGGAAG TTCCACCTGG AGTACCGCAA GACAGATGCC 
TTTCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCG 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC ATGGTGGATG ACAAGAGCGA AGATTCCATG 
TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC AGACCCACAA TCTCCTGCAT CTTTGACTAT 
GGGAACCGCT ACCATCTGCG TTGCTACCTG TACCAGGCCC GGGACCTGCC CGCCATGGAC 
AAAGACTCCT TCTCTGATCC CTATGCCATT GTCTCCTTCC TGCATCAAAG CCAGAAGACG 
GTGGTGGAGA AGAACACCCT GAACCCCACC TGGGATCAAA CCCTCATCTT CTATGAGATA 
GAGATCTTTG GCGAGCCGGC CAGTGTCGCC GAGCGGCCAC CCAGCATTGT GGTGGAGCTG 
TATGACCATG ACACCTATGG CGCTGACGAG TTCATGGGCC GCTGTATTTG TCAGCCAAGC 
CTGGAACGGA TGCCCCGCCT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCTGCCGGA 
GAGCTGCTGG CAGCGTTCGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA TCACATCCCT 
GGCTTTGAGA TGCACGAAAC TTCAAGGATT TTGGATGAGC TGTGCGCTCC TGCCTTCTTC 
CTGCAGGGCC TACTCCAGAC GACGGAGGAC ACAGACTTGC CTTACCCACC ACCTCAAAGG 
GAGGCCAACA TCTACATGGT TCCCCAGAAC ATCAAGCCAG CTCTCCAGCG TACAGCCATC 
GAGATCCTGG CCTGGGGCCT TCGGAACATA AAGAGCTATC AGATGGCCAG CGTGTCTTCC 
CCCAGCCTCG TGGTGGAGTG TGGTGGGCAG ACAGTGCAGT CCTGTGTCAT CAGAAACCTT 
CGGAAGAACC CCAACTTTGA CGTCTGTACT CTGTTCATGG AAGTGATGTT ACCCAGAGAG 
GACCTCTACT GTCCCCCTAT TGTGGTCAAG GTCATTGATA ATCGCCAGTT TGGCCGCAGG 
CCAGTTGTGG GTCAGTGCAC CATTCGCTCC CTGGAGAACT TCCTGTGTGA CCCGTACTCA 
GCAGAGAGTC CATCCCCGCA AGGTGGCCCA GATGACGTGA GCTTACTCAG CCCTGGGGAA 
GACGTGCTCA TTGACATTGA TGACAAGGAG CCTCTCATCC CTGTCCAGCT TGCAGATGGG 
CTGTCAAGCT TGGGCCCCAC TAACCTGATG CCTTCTCCGT CCAGTCCTCA TGAGGAGGAG 
TTCATTGACT GGTGGAGCAA ATTCTTTGCT TCTATCGGGG AGAGGGAAAA GTGTGGTTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGGAG GCCTGTCCGA CTTTTGTAAT ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGACACAG AAGATCCATC TGTCATCGGA GAATTTAAGG GTCTCTTCAA AATTTATCCC 
CTCCCAGAAG ATCCAGCCAT CCCTATGCCC CCGAGACAAT TCCATCAGCT GGCTGCTCAG 
GGTCCCCAGG AATGCTTGGT CCGGATTTAC ATCATCCGAG CATTTGGCTT ACAGCCCAAG 
GACCCCAATG GCAAGTGCGA CCCTTACATC AAGATCTCTA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC TTGTACCCTG GAGCCTGTAT TTGGAAAGAT GTTTGAACTC 
ACCTGCACTC TACCTCTGGA GAAGGACTTG AAGATCACAC TCTATGACTA TGACCTGCTC 
TCCAAGGATG AGAAGATTGG GGAGACAGTC ATTGATCTGG AGAATAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGCCT TCCACAGACC TACTGTGTCT CTGGACCAAA TCAGTGGAGA 
GATCAACTCC GCCCTTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCACAG GGTCAAGGCC 
CCCGTGTACC GGACAGACCG AGTGACGTTT CAGGATAAGG AATACACCAT TCAGGAGATA 
GAGGCTGGCA GACTCCCAAA CCCACACCTG GGCCCGGTGG AGGAACGCTT AGCCCTGCAT 
GTCCTTCAAC AACAAGGCTT GGTCCCTGAG CATGTGGAGT CTCGGCCTCT GTACAGTCCT 
CTGCAGCCAG ATATTGAGCA GGGGAAGCTA CAGATGTGGA TTGACATATT TCCAAAGGTG 
CTGGGCCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA AAGCTCGAAG GTTTTTTCTG 
CGTTGTATTA TTTGGAACAC AAAAGATGTG ATCTTGGATG ACCTCAGCCT CACGGGGGAG 
AAGATGAGTG ACATCTATGT GAAAGGCTGG ATGGTGGGAT TCGAAGAACA CAAACAGAAG 
ACAGATGTGC ACTACCGTTC CCTGGGCGGG GAGGGCAATT TCAACTGGAG GTTCGTGTTT 
CCCTTCGACT ATCTGCCCGC TGAGCAAGTC TGTGCTGTTG CCAAGAAGGA TGCTTTCTGG 
AGACTCGACA AGACAGAGAG CAAGATCCCA GCACGTGTGG TCTTCCAGAT TTGGGACAAT 
GACAAGTTCT CCTTTGATGA CTTTCTGGGC TCCCTGCAGC TGGATCTCAA CCGAATGCCC 
AAGCCAGCCA AGACGGCTGA GAAGTGCTCC TTGGACCAGC TGGATGACAC CTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAG ACAGTGAAAG GATGGTGGCC TTGTGTGACA 
GAGGAGGGCG CGAAGAAGAT CCTGGCGGGG AAGCTGGAAA TGACCTTGGA GATCGTTGCA 
GAAAGTGAAC ATGAGGAGCG GCCTGCTGGC CAGGGTCGGG ATGAACCCAA CATGAATCCA 
AAGCTAGAAG ATCCCAGGCG CCCTGACACC TCTTTCCTGT GGTTCACCTC ACCATACAAG 
ACCATGAAGT TCATCTTGTG GCGACGATTC CGGTGTGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTCC TGCTCTTCCT GGGTGTCTTT GTCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_017592631.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448120.1 Rattus norvegicus dysferlin (Dysf), transcript variant X3, mRNA.

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CloneID	ORa60859
Clone ID Related Accession (Same CDS sequence)	XM_017592631.1
Accession Version	XM_017592631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGTGGATGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCG 
GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCATATAGGG GCAGGCTTCT GTTGTCCCTG 
GAGACCAAAC TGGTGGAGCA CAGTGAACAG AAGGTGGAAG ACCTTCCTGC TGATGACATC 
TTGAGAGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA 
GCCACAATGC TGCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC 
GGAAACAAGT TTGACACCAC CTGCTTGCCG TTGGCCTCTA CCACCCAATA CAGCCGGGCA 
GTCTTTGATG GATGCCACTA CTATTATTTG CCCTGGGGCA ACGTGAAGCC TGTGGTAGTA 
CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAGTCGAGA CTCAGAACCA GCTCCTCAGG 
ATCGCTGACC GACTGGAAGC TAACCTGGAG CAAGTCCACC TGGCTCTGAA GGCACAGTGT 
TCCTCTGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC 
AGCCAGCCCC TGTGTGACAT CCATGAAATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG 
TTCCGCCTCC GCTCCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCCCTGGC CTTGAAGCTT 
GGCCACAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT 
GCCCTGGCTG AGGAGCCCCA GAACAGCCTG CCAGATATCA TCATCTGGAT GCTGCAGGGT 
GACAAGCGTG TGGCCTACCA ACGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC 
CCCAGCTACT GCGGCAGGAA CTGTGGGAAT CTGCAGACCA TCTTTCTGAA ATATCCTATG 
GAAGGCATGC CTGGGCCCCG GATGCCGGTG CAGATCCGGA TCAAGCTCTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAATT CAATCAGTTT GCAGAGGGGA AGCTCTCTGT CTTTGCTGAA 
ACGTATGAGA ACCAGACAAA GCTGGCGCTG GTCGGGAACT GGGGCACGAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAACTAC CGAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC AGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GATGCTGACG CCGGTCATCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACTCGGCTA 
CCTGGAGGCC AGTGGATCTA TATGAGTGAC AACTACACCG ACGTGAATGG GGAGAAGGTG 
CTTCCAAAGG ACGATATTGA ATGCCCGCTG GGTTGGAAGT GGGAAGATGA AGAATGGTCC 
ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA 
GACCGAAAGC CCAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA 
CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AGGCACTGAA GAAGCACAGG 
CAGGCAGAGG CTGAGGGCGA GGGCTGGGAA TACGCCTCTC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA TGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCGGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCATGGTG 
GATGACAAGA GCGAAGATTC CATGTCTGTG TCTACCCTGA GCTTTGGCGT TAACAGACCC 
ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGTTGCTA CCTGTACCAG 
GCCCGGGACC TGCCCGCCAT GGACAAAGAC TCCTTCTCTG ATCCCTATGC CATTGTCTCC 
TTCCTGCATC AAAGCCAGAA GACGGTGGTG GAGAAGAACA CCCTGAACCC CACCTGGGAT 
CAAACCCTCA TCTTCTATGA GATAGAGATC TTTGGCGAGC CGGCCAGTGT CGCCGAGCGG 
CCACCCAGCA TTGTGGTGGA GCTGTATGAC CATGACACCT ATGGCGCTGA CGAGTTCATG 
GGCCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCTGC CGGAGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCACG AAACTTCAAG GATTTTGGAT 
GAGCTGTGCG CTCCTGCCTT CTTCCTGCAG GGCCTACTCC AGACGACGGA GGACACAGAC 
TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCAGCTCTCC AGCGTACAGC CATCGAGATC CTGGCCTGGG GCCTTCGGAA CATAAAGAGC 
TATCAGATGG CCAGCGTGTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG 
CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGACGTCTG TACTCTGTTC 
ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCCC CTATTGTGGT CAAGGTCATT 
GATAATCGCC AGTTTGGCCG CAGGCCAGTT GTGGGTCAGT GCACCATTCG CTCCCTGGAG 
AACTTCCTGT GTGACCCGTA CTCAGCAGAG AGTCCATCCC CGCAAGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGCCCTGG GGAAGACGTG CTCATTGACA TTGATGACAA GGAGCCTCTC 
ATCCCTGTCC AGCTTGCAGA TGGGCTGTCA AGCTTGGGCC CCACTAACCT GATGCCTTCT 
CCGTCCAGTC CTCATGAGGA GGAGTTCATT GACTGGTGGA GCAAATTCTT TGCTTCTATC 
GGGGAGAGGG AAAAGTGTGG TTCCTACCTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GGAGGCCTGT CCGACTTTTG TAATACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAC ACAGAAGATC CATCTGTCAT CGGAGAATTT 
AAGGGTCTCT TCAAAATTTA TCCCCTCCCA GAAGATCCAG CCATCCCTAT GCCCCCGAGA 
CAATTCCATC AGCTGGCTGC TCAGGGTCCC CAGGAATGCT TGGTCCGGAT TTACATCATC 
CGAGCATTTG GCTTACAGCC CAAGGACCCC AATGGCAAGT GCGACCCTTA CATCAAGATC 
TCTATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCTTGTAC CCTGGAGCCT 
GTATTTGGAA AGATGTTTGA ACTCACCTGC ACTCTACCTC TGGAGAAGGA CTTGAAGATC 
ACACTCTATG ACTATGACCT GCTCTCCAAG GATGAGAAGA TTGGGGAGAC AGTCATTGAT 
CTGGAGAATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GCCTTCCACA GACCTACTGT 
GTCTCTGGAC CAAATCAGTG GAGAGATCAA CTCCGCCCTT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC ACAGGGTCAA GGCCCCCGTG TACCGGACAG ACCGAGTGAC GTTTCAGGAT 
AAGGAATACA CCATTCAGGA GATAGAGGCT GGCAGACTCC CAAACCCACA CCTGGGCCCG 
GTGGAGGAAC GCTTAGCCCT GCATGTCCTT CAACAACAAG GCTTGGTCCC TGAGCATGTG 
GAGTCTCGGC CTCTGTACAG TCCTCTGCAG CCAGATATTG AGCAGGGGAA GCTACAGATG 
TGGATTGACA TATTTCCAAA GGTGCTGGGC CGGCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAAAGCTC GAAGGTTTTT TCTGCGTTGT ATTATTTGGA ACACAAAAGA TGTGATCTTG 
GATGACCTCA GCCTCACGGG GGAGAAGATG AGTGACATCT ATGTGAAAGG CTGGATGGTG 
GGATTCGAAG AACACAAACA GAAGACAGAT GTGCACTACC GTTCCCTGGG CGGGGAGGGC 
AATTTCAACT GGAGGTTCGT GTTTCCCTTC GACTATCTGC CCGCTGAGCA AGTCTGTGCT 
GTTGCCAAGA AGGATGCTTT CTGGAGACTC GACAAGACAG AGAGCAAGAT CCCAGCACGT 
GTGGTCTTCC AGATTTGGGA CAATGACAAG TTCTCCTTTG ATGACTTTCT GGGCTCCCTG 
CAGCTGGATC TCAACCGAAT GCCCAAGCCA GCCAAGACGG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACCTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAGACAGTG 
AAAGGATGGT GGCCTTGTGT GACAGAGGAG GGCGCGAAGA AGATCCTGGC GGGGAAGCTG 
GAAATGACCT TGGAGATCGT TGCAGAAAGT GAACATGAGG AGCGGCCTGC TGGCCAGGGT 
CGGGATGAAC CCAACATGAA TCCAAAGCTA GAAGATCCCA GGCGCCCTGA CACCTCTTTC 
CTGTGGTTCA CCTCACCATA CAAGACCATG AAGTTCATCT TGTGGCGACG ATTCCGGTGT 
GCCATCATCC TCTTCATCAT CCTCTTCATC CTCCTGCTCT TCCTGGGTGT CTTTGTCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448120.1
CDS	703..7110
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDELCAPAFFLQGLLQTTEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_017592631.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592631.1

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGTGGATGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCG 
GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCATATAGGG GCAGGCTTCT GTTGTCCCTG 
GAGACCAAAC TGGTGGAGCA CAGTGAACAG AAGGTGGAAG ACCTTCCTGC TGATGACATC 
TTGAGAGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA 
GCCACAATGC TGCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC 
GGAAACAAGT TTGACACCAC CTGCTTGCCG TTGGCCTCTA CCACCCAATA CAGCCGGGCA 
GTCTTTGATG GATGCCACTA CTATTATTTG CCCTGGGGCA ACGTGAAGCC TGTGGTAGTA 
CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAGTCGAGA CTCAGAACCA GCTCCTCAGG 
ATCGCTGACC GACTGGAAGC TAACCTGGAG CAAGTCCACC TGGCTCTGAA GGCACAGTGT 
TCCTCTGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC 
AGCCAGCCCC TGTGTGACAT CCATGAAATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG 
TTCCGCCTCC GCTCCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCCCTGGC CTTGAAGCTT 
GGCCACAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT 
GCCCTGGCTG AGGAGCCCCA GAACAGCCTG CCAGATATCA TCATCTGGAT GCTGCAGGGT 
GACAAGCGTG TGGCCTACCA ACGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC 
CCCAGCTACT GCGGCAGGAA CTGTGGGAAT CTGCAGACCA TCTTTCTGAA ATATCCTATG 
GAAGGCATGC CTGGGCCCCG GATGCCGGTG CAGATCCGGA TCAAGCTCTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAATT CAATCAGTTT GCAGAGGGGA AGCTCTCTGT CTTTGCTGAA 
ACGTATGAGA ACCAGACAAA GCTGGCGCTG GTCGGGAACT GGGGCACGAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAACTAC CGAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC AGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GATGCTGACG CCGGTCATCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACTCGGCTA 
CCTGGAGGCC AGTGGATCTA TATGAGTGAC AACTACACCG ACGTGAATGG GGAGAAGGTG 
CTTCCAAAGG ACGATATTGA ATGCCCGCTG GGTTGGAAGT GGGAAGATGA AGAATGGTCC 
ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA 
GACCGAAAGC CCAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA 
CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AGGCACTGAA GAAGCACAGG 
CAGGCAGAGG CTGAGGGCGA GGGCTGGGAA TACGCCTCTC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA TGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCGGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCATGGTG 
GATGACAAGA GCGAAGATTC CATGTCTGTG TCTACCCTGA GCTTTGGCGT TAACAGACCC 
ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGTTGCTA CCTGTACCAG 
GCCCGGGACC TGCCCGCCAT GGACAAAGAC TCCTTCTCTG ATCCCTATGC CATTGTCTCC 
TTCCTGCATC AAAGCCAGAA GACGGTGGTG GAGAAGAACA CCCTGAACCC CACCTGGGAT 
CAAACCCTCA TCTTCTATGA GATAGAGATC TTTGGCGAGC CGGCCAGTGT CGCCGAGCGG 
CCACCCAGCA TTGTGGTGGA GCTGTATGAC CATGACACCT ATGGCGCTGA CGAGTTCATG 
GGCCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCTGC CGGAGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCACG AAACTTCAAG GATTTTGGAT 
GAGCTGTGCG CTCCTGCCTT CTTCCTGCAG GGCCTACTCC AGACGACGGA GGACACAGAC 
TTGCCTTACC CACCACCTCA AAGGGAGGCC AACATCTACA TGGTTCCCCA GAACATCAAG 
CCAGCTCTCC AGCGTACAGC CATCGAGATC CTGGCCTGGG GCCTTCGGAA CATAAAGAGC 
TATCAGATGG CCAGCGTGTC TTCCCCCAGC CTCGTGGTGG AGTGTGGTGG GCAGACAGTG 
CAGTCCTGTG TCATCAGAAA CCTTCGGAAG AACCCCAACT TTGACGTCTG TACTCTGTTC 
ATGGAAGTGA TGTTACCCAG AGAGGACCTC TACTGTCCCC CTATTGTGGT CAAGGTCATT 
GATAATCGCC AGTTTGGCCG CAGGCCAGTT GTGGGTCAGT GCACCATTCG CTCCCTGGAG 
AACTTCCTGT GTGACCCGTA CTCAGCAGAG AGTCCATCCC CGCAAGGTGG CCCAGATGAC 
GTGAGCTTAC TCAGCCCTGG GGAAGACGTG CTCATTGACA TTGATGACAA GGAGCCTCTC 
ATCCCTGTCC AGCTTGCAGA TGGGCTGTCA AGCTTGGGCC CCACTAACCT GATGCCTTCT 
CCGTCCAGTC CTCATGAGGA GGAGTTCATT GACTGGTGGA GCAAATTCTT TGCTTCTATC 
GGGGAGAGGG AAAAGTGTGG TTCCTACCTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GGAGGCCTGT CCGACTTTTG TAATACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAC ACAGAAGATC CATCTGTCAT CGGAGAATTT 
AAGGGTCTCT TCAAAATTTA TCCCCTCCCA GAAGATCCAG CCATCCCTAT GCCCCCGAGA 
CAATTCCATC AGCTGGCTGC TCAGGGTCCC CAGGAATGCT TGGTCCGGAT TTACATCATC 
CGAGCATTTG GCTTACAGCC CAAGGACCCC AATGGCAAGT GCGACCCTTA CATCAAGATC 
TCTATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCTTGTAC CCTGGAGCCT 
GTATTTGGAA AGATGTTTGA ACTCACCTGC ACTCTACCTC TGGAGAAGGA CTTGAAGATC 
ACACTCTATG ACTATGACCT GCTCTCCAAG GATGAGAAGA TTGGGGAGAC AGTCATTGAT 
CTGGAGAATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GCCTTCCACA GACCTACTGT 
GTCTCTGGAC CAAATCAGTG GAGAGATCAA CTCCGCCCTT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC ACAGGGTCAA GGCCCCCGTG TACCGGACAG ACCGAGTGAC GTTTCAGGAT 
AAGGAATACA CCATTCAGGA GATAGAGGCT GGCAGACTCC CAAACCCACA CCTGGGCCCG 
GTGGAGGAAC GCTTAGCCCT GCATGTCCTT CAACAACAAG GCTTGGTCCC TGAGCATGTG 
GAGTCTCGGC CTCTGTACAG TCCTCTGCAG CCAGATATTG AGCAGGGGAA GCTACAGATG 
TGGATTGACA TATTTCCAAA GGTGCTGGGC CGGCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAAAGCTC GAAGGTTTTT TCTGCGTTGT ATTATTTGGA ACACAAAAGA TGTGATCTTG 
GATGACCTCA GCCTCACGGG GGAGAAGATG AGTGACATCT ATGTGAAAGG CTGGATGGTG 
GGATTCGAAG AACACAAACA GAAGACAGAT GTGCACTACC GTTCCCTGGG CGGGGAGGGC 
AATTTCAACT GGAGGTTCGT GTTTCCCTTC GACTATCTGC CCGCTGAGCA AGTCTGTGCT 
GTTGCCAAGA AGGATGCTTT CTGGAGACTC GACAAGACAG AGAGCAAGAT CCCAGCACGT 
GTGGTCTTCC AGATTTGGGA CAATGACAAG TTCTCCTTTG ATGACTTTCT GGGCTCCCTG 
CAGCTGGATC TCAACCGAAT GCCCAAGCCA GCCAAGACGG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACCTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAGACAGTG 
AAAGGATGGT GGCCTTGTGT GACAGAGGAG GGCGCGAAGA AGATCCTGGC GGGGAAGCTG 
GAAATGACCT TGGAGATCGT TGCAGAAAGT GAACATGAGG AGCGGCCTGC TGGCCAGGGT 
CGGGATGAAC CCAACATGAA TCCAAAGCTA GAAGATCCCA GGCGCCCTGA CACCTCTTTC 
CTGTGGTTCA CCTCACCATA CAAGACCATG AAGTTCATCT TGTGGCGACG ATTCCGGTGT 
GCCATCATCC TCTTCATCAT CCTCTTCATC CTCCTGCTCT TCCTGGGTGT CTTTGTCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_017592632.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448121.1 Rattus norvegicus dysferlin (Dysf), transcript variant X5, mRNA.

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CloneID	ORa60860
Clone ID Related Accession (Same CDS sequence)	XM_017592632.1
Accession Version	XM_017592632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGCTGTGCGC TCCTGCCTTC 
TTCCTGCAGG GCCTACTCCA GACGACGGAG GACACAGACT TGCCTTACCC ACCACCTCAA 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGCTCTCCA GCGTACAGCC 
ATCGAGATCC TGGCCTGGGG CCTTCGGAAC ATAAAGAGCT ATCAGATGGC CAGCGTGTCT 
TCCCCCAGCC TCGTGGTGGA GTGTGGTGGG CAGACAGTGC AGTCCTGTGT CATCAGAAAC 
CTTCGGAAGA ACCCCAACTT TGACGTCTGT ACTCTGTTCA TGGAAGTGAT GTTACCCAGA 
GAGGACCTCT ACTGTCCCCC TATTGTGGTC AAGGTCATTG ATAATCGCCA GTTTGGCCGC 
AGGCCAGTTG TGGGTCAGTG CACCATTCGC TCCCTGGAGA ACTTCCTGTG TGACCCGTAC 
TCAGCAGAGA GTCCATCCCC GCAAGGTGGC CCAGATGACG TGAGCTTACT CAGCCCTGGG 
GAAGACGTGC TCATTGACAT TGATGACAAG GAGCCTCTCA TCCCTGTCCA GGAGGAGGAG 
TTCATTGACT GGTGGAGCAA ATTCTTTGCT TCTATCGGGG AGAGGGAAAA GTGTGGTTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGGAG GCCTGTCCGA CTTTTGTAAT ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGACACAG AAGATCCATC TGTCATCGGA GAATTTAAGG GTCTCTTCAA AATTTATCCC 
CTCCCAGAAG ATCCAGCCAT CCCTATGCCC CCGAGACAAT TCCATCAGCT GGCTGCTCAG 
GGTCCCCAGG AATGCTTGGT CCGGATTTAC ATCATCCGAG CATTTGGCTT ACAGCCCAAG 
GACCCCAATG GCAAGTGCGA CCCTTACATC AAGATCTCTA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC TTGTACCCTG GAGCCTGTAT TTGGAAAGAT GTTTGAACTC 
ACCTGCACTC TACCTCTGGA GAAGGACTTG AAGATCACAC TCTATGACTA TGACCTGCTC 
TCCAAGGATG AGAAGATTGG GGAGACAGTC ATTGATCTGG AGAATAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGCCT TCCACAGACC TACTGTGTCT CTGGACCAAA TCAGTGGAGA 
GATCAACTCC GCCCTTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCACAG GGTCAAGGCC 
CCCGTGTACC GGACAGACCG AGTGACGTTT CAGGATAAGG AATACACCAT TCAGGAGATA 
GAGGCTGGCA GACTCCCAAA CCCACACCTG GGCCCGGTGG AGGAACGCTT AGCCCTGCAT 
GTCCTTCAAC AACAAGGCTT GGTCCCTGAG CATGTGGAGT CTCGGCCTCT GTACAGTCCT 
CTGCAGCCAG ATATTGAGCA GGGGAAGCTA CAGATGTGGA TTGACATATT TCCAAAGGTG 
CTGGGCCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA AAGCTCGAAG GTTTTTTCTG 
CGTTGTATTA TTTGGAACAC AAAAGATGTG ATCTTGGATG ACCTCAGCCT CACGGGGGAG 
AAGATGAGTG ACATCTATGT GAAAGGCTGG ATGGTGGGAT TCGAAGAACA CAAACAGAAG 
ACAGATGTGC ACTACCGTTC CCTGGGCGGG GAGGGCAATT TCAACTGGAG GTTCGTGTTT 
CCCTTCGACT ATCTGCCCGC TGAGCAAGTC TGTGCTGTTG CCAAGAAGGA TGCTTTCTGG 
AGACTCGACA AGACAGAGAG CAAGATCCCA GCACGTGTGG TCTTCCAGAT TTGGGACAAT 
GACAAGTTCT CCTTTGATGA CTTTCTGGGC TCCCTGCAGC TGGATCTCAA CCGAATGCCC 
AAGCCAGCCA AGACGGCTGA GAAGTGCTCC TTGGACCAGC TGGATGACAC CTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAG ACAGTGAAAG GATGGTGGCC TTGTGTGACA 
GAGGAGGGCG CGAAGAAGAT CCTGGCGGGG AAGCTGGAAA TGACCTTGGA GATCGTTGCA 
GAAAGTGAAC ATGAGGAGCG GCCTGCTGGC CAGGGTCGGG ATGAACCCAA CATGAATCCA 
AAGCTAGAAG ATCCCAGGCG CCCTGACACC TCTTTCCTGT GGTTCACCTC ACCATACAAG 
ACCATGAAGT TCATCTTGTG GCGACGATTC CGGTGTGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTCC TGCTCTTCCT GGGTGTCTTT GTCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448121.1
CDS	703..7086
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDELCAPAFFLQGLLQTTEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_017592632.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592632.1

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGCTGTGCGC TCCTGCCTTC 
TTCCTGCAGG GCCTACTCCA GACGACGGAG GACACAGACT TGCCTTACCC ACCACCTCAA 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGCTCTCCA GCGTACAGCC 
ATCGAGATCC TGGCCTGGGG CCTTCGGAAC ATAAAGAGCT ATCAGATGGC CAGCGTGTCT 
TCCCCCAGCC TCGTGGTGGA GTGTGGTGGG CAGACAGTGC AGTCCTGTGT CATCAGAAAC 
CTTCGGAAGA ACCCCAACTT TGACGTCTGT ACTCTGTTCA TGGAAGTGAT GTTACCCAGA 
GAGGACCTCT ACTGTCCCCC TATTGTGGTC AAGGTCATTG ATAATCGCCA GTTTGGCCGC 
AGGCCAGTTG TGGGTCAGTG CACCATTCGC TCCCTGGAGA ACTTCCTGTG TGACCCGTAC 
TCAGCAGAGA GTCCATCCCC GCAAGGTGGC CCAGATGACG TGAGCTTACT CAGCCCTGGG 
GAAGACGTGC TCATTGACAT TGATGACAAG GAGCCTCTCA TCCCTGTCCA GGAGGAGGAG 
TTCATTGACT GGTGGAGCAA ATTCTTTGCT TCTATCGGGG AGAGGGAAAA GTGTGGTTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGGAG GCCTGTCCGA CTTTTGTAAT ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGACACAG AAGATCCATC TGTCATCGGA GAATTTAAGG GTCTCTTCAA AATTTATCCC 
CTCCCAGAAG ATCCAGCCAT CCCTATGCCC CCGAGACAAT TCCATCAGCT GGCTGCTCAG 
GGTCCCCAGG AATGCTTGGT CCGGATTTAC ATCATCCGAG CATTTGGCTT ACAGCCCAAG 
GACCCCAATG GCAAGTGCGA CCCTTACATC AAGATCTCTA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC TTGTACCCTG GAGCCTGTAT TTGGAAAGAT GTTTGAACTC 
ACCTGCACTC TACCTCTGGA GAAGGACTTG AAGATCACAC TCTATGACTA TGACCTGCTC 
TCCAAGGATG AGAAGATTGG GGAGACAGTC ATTGATCTGG AGAATAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGCCT TCCACAGACC TACTGTGTCT CTGGACCAAA TCAGTGGAGA 
GATCAACTCC GCCCTTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCACAG GGTCAAGGCC 
CCCGTGTACC GGACAGACCG AGTGACGTTT CAGGATAAGG AATACACCAT TCAGGAGATA 
GAGGCTGGCA GACTCCCAAA CCCACACCTG GGCCCGGTGG AGGAACGCTT AGCCCTGCAT 
GTCCTTCAAC AACAAGGCTT GGTCCCTGAG CATGTGGAGT CTCGGCCTCT GTACAGTCCT 
CTGCAGCCAG ATATTGAGCA GGGGAAGCTA CAGATGTGGA TTGACATATT TCCAAAGGTG 
CTGGGCCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA AAGCTCGAAG GTTTTTTCTG 
CGTTGTATTA TTTGGAACAC AAAAGATGTG ATCTTGGATG ACCTCAGCCT CACGGGGGAG 
AAGATGAGTG ACATCTATGT GAAAGGCTGG ATGGTGGGAT TCGAAGAACA CAAACAGAAG 
ACAGATGTGC ACTACCGTTC CCTGGGCGGG GAGGGCAATT TCAACTGGAG GTTCGTGTTT 
CCCTTCGACT ATCTGCCCGC TGAGCAAGTC TGTGCTGTTG CCAAGAAGGA TGCTTTCTGG 
AGACTCGACA AGACAGAGAG CAAGATCCCA GCACGTGTGG TCTTCCAGAT TTGGGACAAT 
GACAAGTTCT CCTTTGATGA CTTTCTGGGC TCCCTGCAGC TGGATCTCAA CCGAATGCCC 
AAGCCAGCCA AGACGGCTGA GAAGTGCTCC TTGGACCAGC TGGATGACAC CTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAG ACAGTGAAAG GATGGTGGCC TTGTGTGACA 
GAGGAGGGCG CGAAGAAGAT CCTGGCGGGG AAGCTGGAAA TGACCTTGGA GATCGTTGCA 
GAAAGTGAAC ATGAGGAGCG GCCTGCTGGC CAGGGTCGGG ATGAACCCAA CATGAATCCA 
AAGCTAGAAG ATCCCAGGCG CCCTGACACC TCTTTCCTGT GGTTCACCTC ACCATACAAG 
ACCATGAAGT TCATCTTGTG GCGACGATTC CGGTGTGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTCC TGCTCTTCCT GGGTGTCTTT GTCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_017592633.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448122.1 Rattus norvegicus dysferlin (Dysf), transcript variant X8, mRNA.

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CloneID	ORa60861
Clone ID Related Accession (Same CDS sequence)	XM_017592633.1
Accession Version	XM_017592633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTACTCA AGAGTCCAGC CACTGACCTG GATGACAACC TGGGCTTCCT CCCCACTTTT 
GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT 
TATGCGGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG 
TCCCTGGAGA CCAAACTGGT GGAGCACAGT GAACAGAAGG TGGAAGACCT TCCTGCTGAT 
GACATCTTGA GAGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC 
TACTCAGCCA CAATGCTGCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATTGGG 
AACTACGGAA ACAAGTTTGA CACCACCTGC TTGCCGTTGG CCTCTACCAC CCAATACAGC 
CGGGCAGTCT TTGATGGATG CCACTACTAT TATTTGCCCT GGGGCAACGT GAAGCCTGTG 
GTAGTACTGT CCTCATACTG GGAAGACATC AGCCATCGAG TCGAGACTCA GAACCAGCTC 
CTCAGGATCG CTGACCGACT GGAAGCTAAC CTGGAGCAAG TCCACCTGGC TCTGAAGGCA 
CAGTGTTCCT CTGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA 
GACTGCAGCC AGCCCCTGTG TGACATCCAT GAAATACCTT CTGCCACCCA CCTTGATCAG 
TACCTGTTCC GCCTCCGCTC CCGTCACCTG AGCCAGATCA AAGAAGCTGC CCTGGCCTTG 
AAGCTTGGCC ACAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT 
CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC AGCCTGCCAG ATATCATCAT CTGGATGCTG 
CAGGGTGACA AGCGTGTGGC CTACCAACGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG 
CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAATCTGC AGACCATCTT TCTGAAATAT 
CCTATGGAAG GCATGCCTGG GCCCCGGATG CCGGTGCAGA TCCGGATCAA GCTCTGGTTT 
GGGCTCTCAG TGGATGAGAA GGAATTCAAT CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT 
GCTGAAACGT ATGAGAACCA GACAAAGCTG GCGCTGGTCG GGAACTGGGG CACGACGGGC 
CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AACTACCGAA GGATAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGATG CTGACGCCGG TCATCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACT 
CGGCTACCTG GAGGCCAGTG GATCTATATG AGTGACAACT ACACCGACGT GAATGGGGAG 
AAGGTGCTTC CAAAGGACGA TATTGAATGC CCGCTGGGTT GGAAGTGGGA AGATGAAGAA 
TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA 
CCCCCAGACC GAAAGCCCAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG 
CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAAG 
CACAGGCAGG CAGAGGCTGA GGGCGAGGGC TGGGAATACG CCTCTCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGATGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCACTGG AGAAGACGGG GCCTGCAGCG GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC 
ATGGTGGATG ACAAGAGCGA AGATTCCATG TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC 
AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG TTGCTACCTG 
TACCAGGCCC GGGACCTGCC CGCCATGGAC AAAGACTCCT TCTCTGATCC CTATGCCATT 
GTCTCCTTCC TGCATCAAAG CCAGAAGACG GTGGTGGAGA AGAACACCCT GAACCCCACC 
TGGGATCAAA CCCTCATCTT CTATGAGATA GAGATCTTTG GCGAGCCGGC CAGTGTCGCC 
GAGCGGCCAC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTATGG CGCTGACGAG 
TTCATGGGCC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC 
CCACTGACCA GGGGCAGCCA GCCTGCCGGA GAGCTGCTGG CAGCGTTCGA GCTTATCCAG 
AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCACGAAAC TTCAAGGATT 
TTGGATGAGC TGTGCGCTCC TGCCTTCTTC CTGCAGGGCC TACTCCAGAC GACGGAGGAC 
ACAGACTTGC CTTACCCACC ACCTCAAAGG GAGGCCAACA TCTACATGGT TCCCCAGAAC 
ATCAAGCCAG CTCTCCAGCG TACAGCCATC GAGATCCTGG CCTGGGGCCT TCGGAACATA 
AAGAGCTATC AGATGGCCAG CGTGTCTTCC CCCAGCCTCG TGGTGGAGTG TGGTGGGCAG 
ACAGTGCAGT CCTGTGTCAT CAGAAACCTT CGGAAGAACC CCAACTTTGA CGTCTGTACT 
CTGTTCATGG AAGTGATGTT ACCCAGAGAG GACCTCTACT GTCCCCCTAT TGTGGTCAAG 
GTCATTGATA ATCGCCAGTT TGGCCGCAGG CCAGTTGTGG GTCAGTGCAC CATTCGCTCC 
CTGGAGAACT TCCTGTGTGA CCCGTACTCA GCAGAGAGTC CATCCCCGCA AGGTGGCCCA 
GATGACGTGA GCTTACTCAG CCCTGGGGAA GACGTGCTCA TTGACATTGA TGACAAGGAG 
CCTCTCATCC CTGTCCAGCT TGCAGATGGG CTGTCAAGCT TGGGCCCCAC TAACCTGATG 
CCTTCTCCGT CCAGTCCTCA TGAGGAGGAG TTCATTGACT GGTGGAGCAA ATTCTTTGCT 
TCTATCGGGG AGAGGGAAAA GTGTGGTTCC TACCTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGGAG GCCTGTCCGA CTTTTGTAAT 
ACCTTCAAGC TCTACCGGGG CAAGACTCAG GAGGACACAG AAGATCCATC TGTCATCGGA 
GAATTTAAGG GTCTCTTCAA AATTTATCCC CTCCCAGAAG ATCCAGCCAT CCCTATGCCC 
CCGAGACAAT TCCATCAGCT GGCTGCTCAG GGTCCCCAGG AATGCTTGGT CCGGATTTAC 
ATCATCCGAG CATTTGGCTT ACAGCCCAAG GACCCCAATG GCAAGTGCGA CCCTTACATC 
AAGATCTCTA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC TTGTACCCTG 
GAGCCTGTAT TTGGAAAGAT GTTTGAACTC ACCTGCACTC TACCTCTGGA GAAGGACTTG 
AAGATCACAC TCTATGACTA TGACCTGCTC TCCAAGGATG AGAAGATTGG GGAGACAGTC 
ATTGATCTGG AGAATAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGCCT TCCACAGACC 
TACTGTGTCT CTGGACCAAA TCAGTGGAGA GATCAACTCC GCCCTTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCACAG GGTCAAGGCC CCCGTGTACC GGACAGACCG AGTGACGTTT 
CAGGATAAGG AATACACCAT TCAGGAGATA GAGGCTGGCA GACTCCCAAA CCCACACCTG 
GGCCCGGTGG AGGAACGCTT AGCCCTGCAT GTCCTTCAAC AACAAGGCTT GGTCCCTGAG 
CATGTGGAGT CTCGGCCTCT GTACAGTCCT CTGCAGCCAG ATATTGAGCA GGGGAAGCTA 
CAGATGTGGA TTGACATATT TCCAAAGGTG CTGGGCCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA AAGCTCGAAG GTTTTTTCTG CGTTGTATTA TTTGGAACAC AAAAGATGTG 
ATCTTGGATG ACCTCAGCCT CACGGGGGAG AAGATGAGTG ACATCTATGT GAAAGGCTGG 
ATGGTGGGAT TCGAAGAACA CAAACAGAAG ACAGATGTGC ACTACCGTTC CCTGGGCGGG 
GAGGGCAATT TCAACTGGAG GTTCGTGTTT CCCTTCGACT ATCTGCCCGC TGAGCAAGTC 
TGTGCTGTTG CCAAGAAGGA TGCTTTCTGG AGACTCGACA AGACAGAGAG CAAGATCCCA 
GCACGTGTGG TCTTCCAGAT TTGGGACAAT GACAAGTTCT CCTTTGATGA CTTTCTGGGC 
TCCCTGCAGC TGGATCTCAA CCGAATGCCC AAGCCAGCCA AGACGGCTGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC CTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAG 
ACAGTGAAAG GATGGTGGCC TTGTGTGACA GAGGAGGGCG CGAAGAAGAT CCTGGCGGGG 
AAGCTGGAAA TGACCTTGGA GATCGTTGCA GAAAGTGAAC ATGAGGAGCG GCCTGCTGGC 
CAGGGTCGGG ATGAACCCAA CATGAATCCA AAGCTAGAAG ATCCCAGGCG CCCTGACACC 
TCTTTCCTGT GGTTCACCTC ACCATACAAG ACCATGAAGT TCATCTTGTG GCGACGATTC 
CGGTGTGCCA TCATCCTCTT CATCATCCTC TTCATCCTCC TGCTCTTCCT GGGTGTCTTT 
GTCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448122.1
CDS	703..7056
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDELCAPAFFLQGLLQTTEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_017592633.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592633.1

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTACTCA AGAGTCCAGC CACTGACCTG GATGACAACC TGGGCTTCCT CCCCACTTTT 
GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT 
TATGCGGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG 
TCCCTGGAGA CCAAACTGGT GGAGCACAGT GAACAGAAGG TGGAAGACCT TCCTGCTGAT 
GACATCTTGA GAGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC 
TACTCAGCCA CAATGCTGCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATTGGG 
AACTACGGAA ACAAGTTTGA CACCACCTGC TTGCCGTTGG CCTCTACCAC CCAATACAGC 
CGGGCAGTCT TTGATGGATG CCACTACTAT TATTTGCCCT GGGGCAACGT GAAGCCTGTG 
GTAGTACTGT CCTCATACTG GGAAGACATC AGCCATCGAG TCGAGACTCA GAACCAGCTC 
CTCAGGATCG CTGACCGACT GGAAGCTAAC CTGGAGCAAG TCCACCTGGC TCTGAAGGCA 
CAGTGTTCCT CTGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA 
GACTGCAGCC AGCCCCTGTG TGACATCCAT GAAATACCTT CTGCCACCCA CCTTGATCAG 
TACCTGTTCC GCCTCCGCTC CCGTCACCTG AGCCAGATCA AAGAAGCTGC CCTGGCCTTG 
AAGCTTGGCC ACAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT 
CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC AGCCTGCCAG ATATCATCAT CTGGATGCTG 
CAGGGTGACA AGCGTGTGGC CTACCAACGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG 
CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAATCTGC AGACCATCTT TCTGAAATAT 
CCTATGGAAG GCATGCCTGG GCCCCGGATG CCGGTGCAGA TCCGGATCAA GCTCTGGTTT 
GGGCTCTCAG TGGATGAGAA GGAATTCAAT CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT 
GCTGAAACGT ATGAGAACCA GACAAAGCTG GCGCTGGTCG GGAACTGGGG CACGACGGGC 
CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AACTACCGAA GGATAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGATG CTGACGCCGG TCATCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACT 
CGGCTACCTG GAGGCCAGTG GATCTATATG AGTGACAACT ACACCGACGT GAATGGGGAG 
AAGGTGCTTC CAAAGGACGA TATTGAATGC CCGCTGGGTT GGAAGTGGGA AGATGAAGAA 
TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA 
CCCCCAGACC GAAAGCCCAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG 
CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAAG 
CACAGGCAGG CAGAGGCTGA GGGCGAGGGC TGGGAATACG CCTCTCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGATGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCACTGG AGAAGACGGG GCCTGCAGCG GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC 
ATGGTGGATG ACAAGAGCGA AGATTCCATG TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC 
AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG TTGCTACCTG 
TACCAGGCCC GGGACCTGCC CGCCATGGAC AAAGACTCCT TCTCTGATCC CTATGCCATT 
GTCTCCTTCC TGCATCAAAG CCAGAAGACG GTGGTGGAGA AGAACACCCT GAACCCCACC 
TGGGATCAAA CCCTCATCTT CTATGAGATA GAGATCTTTG GCGAGCCGGC CAGTGTCGCC 
GAGCGGCCAC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTATGG CGCTGACGAG 
TTCATGGGCC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC 
CCACTGACCA GGGGCAGCCA GCCTGCCGGA GAGCTGCTGG CAGCGTTCGA GCTTATCCAG 
AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCACGAAAC TTCAAGGATT 
TTGGATGAGC TGTGCGCTCC TGCCTTCTTC CTGCAGGGCC TACTCCAGAC GACGGAGGAC 
ACAGACTTGC CTTACCCACC ACCTCAAAGG GAGGCCAACA TCTACATGGT TCCCCAGAAC 
ATCAAGCCAG CTCTCCAGCG TACAGCCATC GAGATCCTGG CCTGGGGCCT TCGGAACATA 
AAGAGCTATC AGATGGCCAG CGTGTCTTCC CCCAGCCTCG TGGTGGAGTG TGGTGGGCAG 
ACAGTGCAGT CCTGTGTCAT CAGAAACCTT CGGAAGAACC CCAACTTTGA CGTCTGTACT 
CTGTTCATGG AAGTGATGTT ACCCAGAGAG GACCTCTACT GTCCCCCTAT TGTGGTCAAG 
GTCATTGATA ATCGCCAGTT TGGCCGCAGG CCAGTTGTGG GTCAGTGCAC CATTCGCTCC 
CTGGAGAACT TCCTGTGTGA CCCGTACTCA GCAGAGAGTC CATCCCCGCA AGGTGGCCCA 
GATGACGTGA GCTTACTCAG CCCTGGGGAA GACGTGCTCA TTGACATTGA TGACAAGGAG 
CCTCTCATCC CTGTCCAGCT TGCAGATGGG CTGTCAAGCT TGGGCCCCAC TAACCTGATG 
CCTTCTCCGT CCAGTCCTCA TGAGGAGGAG TTCATTGACT GGTGGAGCAA ATTCTTTGCT 
TCTATCGGGG AGAGGGAAAA GTGTGGTTCC TACCTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGGAG GCCTGTCCGA CTTTTGTAAT 
ACCTTCAAGC TCTACCGGGG CAAGACTCAG GAGGACACAG AAGATCCATC TGTCATCGGA 
GAATTTAAGG GTCTCTTCAA AATTTATCCC CTCCCAGAAG ATCCAGCCAT CCCTATGCCC 
CCGAGACAAT TCCATCAGCT GGCTGCTCAG GGTCCCCAGG AATGCTTGGT CCGGATTTAC 
ATCATCCGAG CATTTGGCTT ACAGCCCAAG GACCCCAATG GCAAGTGCGA CCCTTACATC 
AAGATCTCTA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC TTGTACCCTG 
GAGCCTGTAT TTGGAAAGAT GTTTGAACTC ACCTGCACTC TACCTCTGGA GAAGGACTTG 
AAGATCACAC TCTATGACTA TGACCTGCTC TCCAAGGATG AGAAGATTGG GGAGACAGTC 
ATTGATCTGG AGAATAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGCCT TCCACAGACC 
TACTGTGTCT CTGGACCAAA TCAGTGGAGA GATCAACTCC GCCCTTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCACAG GGTCAAGGCC CCCGTGTACC GGACAGACCG AGTGACGTTT 
CAGGATAAGG AATACACCAT TCAGGAGATA GAGGCTGGCA GACTCCCAAA CCCACACCTG 
GGCCCGGTGG AGGAACGCTT AGCCCTGCAT GTCCTTCAAC AACAAGGCTT GGTCCCTGAG 
CATGTGGAGT CTCGGCCTCT GTACAGTCCT CTGCAGCCAG ATATTGAGCA GGGGAAGCTA 
CAGATGTGGA TTGACATATT TCCAAAGGTG CTGGGCCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA AAGCTCGAAG GTTTTTTCTG CGTTGTATTA TTTGGAACAC AAAAGATGTG 
ATCTTGGATG ACCTCAGCCT CACGGGGGAG AAGATGAGTG ACATCTATGT GAAAGGCTGG 
ATGGTGGGAT TCGAAGAACA CAAACAGAAG ACAGATGTGC ACTACCGTTC CCTGGGCGGG 
GAGGGCAATT TCAACTGGAG GTTCGTGTTT CCCTTCGACT ATCTGCCCGC TGAGCAAGTC 
TGTGCTGTTG CCAAGAAGGA TGCTTTCTGG AGACTCGACA AGACAGAGAG CAAGATCCCA 
GCACGTGTGG TCTTCCAGAT TTGGGACAAT GACAAGTTCT CCTTTGATGA CTTTCTGGGC 
TCCCTGCAGC TGGATCTCAA CCGAATGCCC AAGCCAGCCA AGACGGCTGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC CTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAG 
ACAGTGAAAG GATGGTGGCC TTGTGTGACA GAGGAGGGCG CGAAGAAGAT CCTGGCGGGG 
AAGCTGGAAA TGACCTTGGA GATCGTTGCA GAAAGTGAAC ATGAGGAGCG GCCTGCTGGC 
CAGGGTCGGG ATGAACCCAA CATGAATCCA AAGCTAGAAG ATCCCAGGCG CCCTGACACC 
TCTTTCCTGT GGTTCACCTC ACCATACAAG ACCATGAAGT TCATCTTGTG GCGACGATTC 
CGGTGTGCCA TCATCCTCTT CATCATCCTC TTCATCCTCC TGCTCTTCCT GGGTGTCTTT 
GTCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_017592634.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448123.1 Rattus norvegicus dysferlin (Dysf), transcript variant X9, mRNA.

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CloneID	ORa60862
Clone ID Related Accession (Same CDS sequence)	XM_017592634.1
Accession Version	XM_017592634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTACTCAAGA GTCCAGCCAC TGACCTGGAT GACAACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTATG TGAACCTCTA TGGCAGTCCC CGAGAGTTCA CGGGCTTCCC AGATCCTTAT 
GCGGAGCTCA ACACAGGAAA GGGAGAAGGT GTGGCATATA GGGGCAGGCT TCTGTTGTCC 
CTGGAGACCA AACTGGTGGA GCACAGTGAA CAGAAGGTGG AAGACCTTCC TGCTGATGAC 
ATCTTGAGAG TGGAGAAGTA CCTGCGGAGG CGCAAATATT CCCTCTTTGC TGCCTTCTAC 
TCAGCCACAA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TACGGAAACA AGTTTGACAC CACCTGCTTG CCGTTGGCCT CTACCACCCA ATACAGCCGG 
GCAGTCTTTG ATGGATGCCA CTACTATTAT TTGCCCTGGG GCAACGTGAA GCCTGTGGTA 
GTACTGTCCT CATACTGGGA AGACATCAGC CATCGAGTCG AGACTCAGAA CCAGCTCCTC 
AGGATCGCTG ACCGACTGGA AGCTAACCTG GAGCAAGTCC ACCTGGCTCT GAAGGCACAG 
TGTTCCTCTG AGGATGTGGA CGCCCTGGTG GCTCAGTTGA CAGATGAGCT CCTTGCAGAC 
TGCAGCCAGC CCCTGTGTGA CATCCATGAA ATACCTTCTG CCACCCACCT TGATCAGTAC 
CTGTTCCGCC TCCGCTCCCG TCACCTGAGC CAGATCAAAG AAGCTGCCCT GGCCTTGAAG 
CTTGGCCACA GTGAGCTCTC CACTGCCCTG GAGCAGGCCG AGGACTGGCT TCTGCATCTC 
CGTGCCCTGG CTGAGGAGCC CCAGAACAGC CTGCCAGATA TCATCATCTG GATGCTGCAG 
GGTGACAAGC GTGTGGCCTA CCAACGGGTG CCTGCCCATG AAGTCCTCTT CTCCCGGCGG 
GGCCCCAGCT ACTGCGGCAG GAACTGTGGG AATCTGCAGA CCATCTTTCT GAAATATCCT 
ATGGAAGGCA TGCCTGGGCC CCGGATGCCG GTGCAGATCC GGATCAAGCT CTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA ATTCAATCAG TTTGCAGAGG GGAAGCTCTC TGTCTTTGCT 
GAAACGTATG AGAACCAGAC AAAGCTGGCG CTGGTCGGGA ACTGGGGCAC GACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACAGGC AAGATCAAAC TACCGAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGGCCTG GGCAGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGATGCTG ACGCCGGTCA TCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACTCGG 
CTACCTGGAG GCCAGTGGAT CTATATGAGT GACAACTACA CCGACGTGAA TGGGGAGAAG 
GTGCTTCCAA AGGACGATAT TGAATGCCCG CTGGGTTGGA AGTGGGAAGA TGAAGAATGG 
TCCACAGACC TCAATCGGGC TGTTGATGAA CAAGGCTGGG AGTACAGCAT CACCATACCC 
CCAGACCGAA AGCCCAAACA CTGGGTCCCT GTTGAGAAGA TGTATTACAC TCACAGGCGT 
CGACGCTGGG TCCGACTGCG CAGGAGGGAC CTCAGCCAGA TGGAGGCACT GAAGAAGCAC 
AGGCAGGCAG AGGCTGAGGG CGAGGGCTGG GAATACGCCT CTCTCTTTGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC AGATGCCTTT CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCGGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCATG 
GTGGATGACA AGAGCGAAGA TTCCATGTCT GTGTCTACCC TGAGCTTTGG CGTTAACAGA 
CCCACAATCT CCTGCATCTT TGACTATGGG AACCGCTACC ATCTGCGTTG CTACCTGTAC 
CAGGCCCGGG ACCTGCCCGC CATGGACAAA GACTCCTTCT CTGATCCCTA TGCCATTGTC 
TCCTTCCTGC ATCAAAGCCA GAAGACGGTG GTGGAGAAGA ACACCCTGAA CCCCACCTGG 
GATCAAACCC TCATCTTCTA TGAGATAGAG ATCTTTGGCG AGCCGGCCAG TGTCGCCGAG 
CGGCCACCCA GCATTGTGGT GGAGCTGTAT GACCATGACA CCTATGGCGC TGACGAGTTC 
ATGGGCCGCT GTATTTGTCA GCCAAGCCTG GAACGGATGC CCCGCCTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC TGCCGGAGAG CTGCTGGCAG CGTTCGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCATCA CATCCCTGGC TTTGAGATGC ACGAAACTTC AAGGATTTTG 
GATGAGCTGT GCGCTCCTGC CTTCTTCCTG CAGGGCCTAC TCCAGACGAC GGAGGACACA 
GACTTGCCTT ACCCACCACC TCAAAGGGAG GCCAACATCT ACATGGTTCC CCAGAACATC 
AAGCCAGCTC TCCAGCGTAC AGCCATCGAG ATCCTGGCCT GGGGCCTTCG GAACATAAAG 
AGCTATCAGA TGGCCAGCGT GTCTTCCCCC AGCCTCGTGG TGGAGTGTGG TGGGCAGACA 
GTGCAGTCCT GTGTCATCAG AAACCTTCGG AAGAACCCCA ACTTTGACGT CTGTACTCTG 
TTCATGGAAG TGATGTTACC CAGAGAGGAC CTCTACTGTC CCCCTATTGT GGTCAAGGTC 
ATTGATAATC GCCAGTTTGG CCGCAGGCCA GTTGTGGGTC AGTGCACCAT TCGCTCCCTG 
GAGAACTTCC TGTGTGACCC GTACTCAGCA GAGAGTCCAT CCCCGCAAGG TGGCCCAGAT 
GACGTGAGCT TACTCAGCCC TGGGGAAGAC GTGCTCATTG ACATTGATGA CAAGGAGCCT 
CTCATCCCTG TCCAGCTTGC AGATGGGCTG TCAAGCTTGG GCCCCACTAA CCTGATGCCT 
TCTCCGTCCA GTCCTCATGA GGAGGAGTTC ATTGACTGGT GGAGCAAATT CTTTGCTTCT 
ATCGGGGAGA GGGAAAAGTG TGGTTCCTAC CTGGAGAAGG ATTTTGACAC CCTGAAGGTC 
TATGACACAC AGCTGGAGAA TGTGGAGGCC TTTGGAGGCC TGTCCGACTT TTGTAATACC 
TTCAAGCTCT ACCGGGGCAA GACTCAGGAG GACACAGAAG ATCCATCTGT CATCGGAGAA 
TTTAAGGGTC TCTTCAAAAT TTATCCCCTC CCAGAAGATC CAGCCATCCC TATGCCCCCG 
AGACAATTCC ATCAGCTGGC TGCTCAGGGT CCCCAGGAAT GCTTGGTCCG GATTTACATC 
ATCCGAGCAT TTGGCTTACA GCCCAAGGAC CCCAATGGCA AGTGCGACCC TTACATCAAG 
ATCTCTATAG GGAAGAAATC AGTGAGTGAC CAGGATAACT ACATCCCTTG TACCCTGGAG 
CCTGTATTTG GAAAGATGTT TGAACTCACC TGCACTCTAC CTCTGGAGAA GGACTTGAAG 
ATCACACTCT ATGACTATGA CCTGCTCTCC AAGGATGAGA AGATTGGGGA GACAGTCATT 
GATCTGGAGA ATAGGCTGCT GTCCAAGTTT GGGGCTCGCT GTGGCCTTCC ACAGACCTAC 
TGTGTCTCTG GACCAAATCA GTGGAGAGAT CAACTCCGCC CTTCCCAGCT CCTCCACCTC 
TTCTGCCAGC AGCACAGGGT CAAGGCCCCC GTGTACCGGA CAGACCGAGT GACGTTTCAG 
GATAAGGAAT ACACCATTCA GGAGATAGAG GCTGGCAGAC TCCCAAACCC ACACCTGGGC 
CCGGTGGAGG AACGCTTAGC CCTGCATGTC CTTCAACAAC AAGGCTTGGT CCCTGAGCAT 
GTGGAGTCTC GGCCTCTGTA CAGTCCTCTG CAGCCAGATA TTGAGCAGGG GAAGCTACAG 
ATGTGGATTG ACATATTTCC AAAGGTGCTG GGCCGGCCTG GACCTCCCTT CAACATCACC 
CCACGGAAAG CTCGAAGGTT TTTTCTGCGT TGTATTATTT GGAACACAAA AGATGTGATC 
TTGGATGACC TCAGCCTCAC GGGGGAGAAG ATGAGTGACA TCTATGTGAA AGGCTGGATG 
GTGGGATTCG AAGAACACAA ACAGAAGACA GATGTGCACT ACCGTTCCCT GGGCGGGGAG 
GGCAATTTCA ACTGGAGGTT CGTGTTTCCC TTCGACTATC TGCCCGCTGA GCAAGTCTGT 
GCTGTTGCCA AGAAGGATGC TTTCTGGAGA CTCGACAAGA CAGAGAGCAA GATCCCAGCA 
CGTGTGGTCT TCCAGATTTG GGACAATGAC AAGTTCTCCT TTGATGACTT TCTGGGCTCC 
CTGCAGCTGG ATCTCAACCG AATGCCCAAG CCAGCCAAGA CGGCTGAGAA GTGCTCCTTG 
GACCAGCTGG ATGACACCTT CCACCCAGAA TGGTTTGTGT CCCTTTTTGA GCAGAAGACA 
GTGAAAGGAT GGTGGCCTTG TGTGACAGAG GAGGGCGCGA AGAAGATCCT GGCGGGGAAG 
CTGGAAATGA CCTTGGAGAT CGTTGCAGAA AGTGAACATG AGGAGCGGCC TGCTGGCCAG 
GGTCGGGATG AACCCAACAT GAATCCAAAG CTAGAAGATC CCAGGCGCCC TGACACCTCT 
TTCCTGTGGT TCACCTCACC ATACAAGACC ATGAAGTTCA TCTTGTGGCG ACGATTCCGG 
TGTGCCATCA TCCTCTTCAT CATCCTCTTC ATCCTCCTGC TCTTCCTGGG TGTCTTTGTC 
TACGCCTTCC CGAACTATGC TGCCATGAAG CTGGTGAAGC CCTTTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448123.1
CDS	403..6753
Translation	MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDELCAPAFFLQGLLQTTEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_017592634.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592634.1

ORF Insert Sequence:

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTACTCAAGA GTCCAGCCAC TGACCTGGAT GACAACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTATG TGAACCTCTA TGGCAGTCCC CGAGAGTTCA CGGGCTTCCC AGATCCTTAT 
GCGGAGCTCA ACACAGGAAA GGGAGAAGGT GTGGCATATA GGGGCAGGCT TCTGTTGTCC 
CTGGAGACCA AACTGGTGGA GCACAGTGAA CAGAAGGTGG AAGACCTTCC TGCTGATGAC 
ATCTTGAGAG TGGAGAAGTA CCTGCGGAGG CGCAAATATT CCCTCTTTGC TGCCTTCTAC 
TCAGCCACAA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TACGGAAACA AGTTTGACAC CACCTGCTTG CCGTTGGCCT CTACCACCCA ATACAGCCGG 
GCAGTCTTTG ATGGATGCCA CTACTATTAT TTGCCCTGGG GCAACGTGAA GCCTGTGGTA 
GTACTGTCCT CATACTGGGA AGACATCAGC CATCGAGTCG AGACTCAGAA CCAGCTCCTC 
AGGATCGCTG ACCGACTGGA AGCTAACCTG GAGCAAGTCC ACCTGGCTCT GAAGGCACAG 
TGTTCCTCTG AGGATGTGGA CGCCCTGGTG GCTCAGTTGA CAGATGAGCT CCTTGCAGAC 
TGCAGCCAGC CCCTGTGTGA CATCCATGAA ATACCTTCTG CCACCCACCT TGATCAGTAC 
CTGTTCCGCC TCCGCTCCCG TCACCTGAGC CAGATCAAAG AAGCTGCCCT GGCCTTGAAG 
CTTGGCCACA GTGAGCTCTC CACTGCCCTG GAGCAGGCCG AGGACTGGCT TCTGCATCTC 
CGTGCCCTGG CTGAGGAGCC CCAGAACAGC CTGCCAGATA TCATCATCTG GATGCTGCAG 
GGTGACAAGC GTGTGGCCTA CCAACGGGTG CCTGCCCATG AAGTCCTCTT CTCCCGGCGG 
GGCCCCAGCT ACTGCGGCAG GAACTGTGGG AATCTGCAGA CCATCTTTCT GAAATATCCT 
ATGGAAGGCA TGCCTGGGCC CCGGATGCCG GTGCAGATCC GGATCAAGCT CTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA ATTCAATCAG TTTGCAGAGG GGAAGCTCTC TGTCTTTGCT 
GAAACGTATG AGAACCAGAC AAAGCTGGCG CTGGTCGGGA ACTGGGGCAC GACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACAGGC AAGATCAAAC TACCGAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGGCCTG GGCAGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGATGCTG ACGCCGGTCA TCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACTCGG 
CTACCTGGAG GCCAGTGGAT CTATATGAGT GACAACTACA CCGACGTGAA TGGGGAGAAG 
GTGCTTCCAA AGGACGATAT TGAATGCCCG CTGGGTTGGA AGTGGGAAGA TGAAGAATGG 
TCCACAGACC TCAATCGGGC TGTTGATGAA CAAGGCTGGG AGTACAGCAT CACCATACCC 
CCAGACCGAA AGCCCAAACA CTGGGTCCCT GTTGAGAAGA TGTATTACAC TCACAGGCGT 
CGACGCTGGG TCCGACTGCG CAGGAGGGAC CTCAGCCAGA TGGAGGCACT GAAGAAGCAC 
AGGCAGGCAG AGGCTGAGGG CGAGGGCTGG GAATACGCCT CTCTCTTTGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC AGATGCCTTT CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCGGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCATG 
GTGGATGACA AGAGCGAAGA TTCCATGTCT GTGTCTACCC TGAGCTTTGG CGTTAACAGA 
CCCACAATCT CCTGCATCTT TGACTATGGG AACCGCTACC ATCTGCGTTG CTACCTGTAC 
CAGGCCCGGG ACCTGCCCGC CATGGACAAA GACTCCTTCT CTGATCCCTA TGCCATTGTC 
TCCTTCCTGC ATCAAAGCCA GAAGACGGTG GTGGAGAAGA ACACCCTGAA CCCCACCTGG 
GATCAAACCC TCATCTTCTA TGAGATAGAG ATCTTTGGCG AGCCGGCCAG TGTCGCCGAG 
CGGCCACCCA GCATTGTGGT GGAGCTGTAT GACCATGACA CCTATGGCGC TGACGAGTTC 
ATGGGCCGCT GTATTTGTCA GCCAAGCCTG GAACGGATGC CCCGCCTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC TGCCGGAGAG CTGCTGGCAG CGTTCGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCATCA CATCCCTGGC TTTGAGATGC ACGAAACTTC AAGGATTTTG 
GATGAGCTGT GCGCTCCTGC CTTCTTCCTG CAGGGCCTAC TCCAGACGAC GGAGGACACA 
GACTTGCCTT ACCCACCACC TCAAAGGGAG GCCAACATCT ACATGGTTCC CCAGAACATC 
AAGCCAGCTC TCCAGCGTAC AGCCATCGAG ATCCTGGCCT GGGGCCTTCG GAACATAAAG 
AGCTATCAGA TGGCCAGCGT GTCTTCCCCC AGCCTCGTGG TGGAGTGTGG TGGGCAGACA 
GTGCAGTCCT GTGTCATCAG AAACCTTCGG AAGAACCCCA ACTTTGACGT CTGTACTCTG 
TTCATGGAAG TGATGTTACC CAGAGAGGAC CTCTACTGTC CCCCTATTGT GGTCAAGGTC 
ATTGATAATC GCCAGTTTGG CCGCAGGCCA GTTGTGGGTC AGTGCACCAT TCGCTCCCTG 
GAGAACTTCC TGTGTGACCC GTACTCAGCA GAGAGTCCAT CCCCGCAAGG TGGCCCAGAT 
GACGTGAGCT TACTCAGCCC TGGGGAAGAC GTGCTCATTG ACATTGATGA CAAGGAGCCT 
CTCATCCCTG TCCAGCTTGC AGATGGGCTG TCAAGCTTGG GCCCCACTAA CCTGATGCCT 
TCTCCGTCCA GTCCTCATGA GGAGGAGTTC ATTGACTGGT GGAGCAAATT CTTTGCTTCT 
ATCGGGGAGA GGGAAAAGTG TGGTTCCTAC CTGGAGAAGG ATTTTGACAC CCTGAAGGTC 
TATGACACAC AGCTGGAGAA TGTGGAGGCC TTTGGAGGCC TGTCCGACTT TTGTAATACC 
TTCAAGCTCT ACCGGGGCAA GACTCAGGAG GACACAGAAG ATCCATCTGT CATCGGAGAA 
TTTAAGGGTC TCTTCAAAAT TTATCCCCTC CCAGAAGATC CAGCCATCCC TATGCCCCCG 
AGACAATTCC ATCAGCTGGC TGCTCAGGGT CCCCAGGAAT GCTTGGTCCG GATTTACATC 
ATCCGAGCAT TTGGCTTACA GCCCAAGGAC CCCAATGGCA AGTGCGACCC TTACATCAAG 
ATCTCTATAG GGAAGAAATC AGTGAGTGAC CAGGATAACT ACATCCCTTG TACCCTGGAG 
CCTGTATTTG GAAAGATGTT TGAACTCACC TGCACTCTAC CTCTGGAGAA GGACTTGAAG 
ATCACACTCT ATGACTATGA CCTGCTCTCC AAGGATGAGA AGATTGGGGA GACAGTCATT 
GATCTGGAGA ATAGGCTGCT GTCCAAGTTT GGGGCTCGCT GTGGCCTTCC ACAGACCTAC 
TGTGTCTCTG GACCAAATCA GTGGAGAGAT CAACTCCGCC CTTCCCAGCT CCTCCACCTC 
TTCTGCCAGC AGCACAGGGT CAAGGCCCCC GTGTACCGGA CAGACCGAGT GACGTTTCAG 
GATAAGGAAT ACACCATTCA GGAGATAGAG GCTGGCAGAC TCCCAAACCC ACACCTGGGC 
CCGGTGGAGG AACGCTTAGC CCTGCATGTC CTTCAACAAC AAGGCTTGGT CCCTGAGCAT 
GTGGAGTCTC GGCCTCTGTA CAGTCCTCTG CAGCCAGATA TTGAGCAGGG GAAGCTACAG 
ATGTGGATTG ACATATTTCC AAAGGTGCTG GGCCGGCCTG GACCTCCCTT CAACATCACC 
CCACGGAAAG CTCGAAGGTT TTTTCTGCGT TGTATTATTT GGAACACAAA AGATGTGATC 
TTGGATGACC TCAGCCTCAC GGGGGAGAAG ATGAGTGACA TCTATGTGAA AGGCTGGATG 
GTGGGATTCG AAGAACACAA ACAGAAGACA GATGTGCACT ACCGTTCCCT GGGCGGGGAG 
GGCAATTTCA ACTGGAGGTT CGTGTTTCCC TTCGACTATC TGCCCGCTGA GCAAGTCTGT 
GCTGTTGCCA AGAAGGATGC TTTCTGGAGA CTCGACAAGA CAGAGAGCAA GATCCCAGCA 
CGTGTGGTCT TCCAGATTTG GGACAATGAC AAGTTCTCCT TTGATGACTT TCTGGGCTCC 
CTGCAGCTGG ATCTCAACCG AATGCCCAAG CCAGCCAAGA CGGCTGAGAA GTGCTCCTTG 
GACCAGCTGG ATGACACCTT CCACCCAGAA TGGTTTGTGT CCCTTTTTGA GCAGAAGACA 
GTGAAAGGAT GGTGGCCTTG TGTGACAGAG GAGGGCGCGA AGAAGATCCT GGCGGGGAAG 
CTGGAAATGA CCTTGGAGAT CGTTGCAGAA AGTGAACATG AGGAGCGGCC TGCTGGCCAG 
GGTCGGGATG AACCCAACAT GAATCCAAAG CTAGAAGATC CCAGGCGCCC TGACACCTCT 
TTCCTGTGGT TCACCTCACC ATACAAGACC ATGAAGTTCA TCTTGTGGCG ACGATTCCGG 
TGTGCCATCA TCCTCTTCAT CATCCTCTTC ATCCTCCTGC TCTTCCTGGG TGTCTTTGTC 
TACGCCTTCC CGAACTATGC TGCCATGAAG CTGGTGAAGC CCTTTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( NM_001107869.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101339.1 Rattus norvegicus dysferlin (Dysf), mRNA.

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CloneID	ORa12046
Clone ID Related Accession (Same CDS sequence)	NM_001107869.1
Accession Version	NM_001107869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473957.1. On or before Oct 4, 2007 this sequence version replaced XM_232123.4, XM_001069038.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GAAGCCAAGA TCCCACTCCA GGAGGTCCTT 
GCCACCCCCA GCCTATCTGC CAGCTTCAAC GCACCTCTGC TGGACTCCAA GCAGCAACCC 
ACAGGGGCCT CTCTGGTCCT GCAGGTATCC TACACACCGC CCCCAGGAGC TGCGCCCTTG 
TTCCCACCAC CCGCTTCCCT GGCACCGTCC CCAACACTGC CTGACATGGA CCTGGTGGCT 
GACACAGGAG GAGAGGAAGA CACGGAAGAC CAGGGCCTGA CTGGAGATGA AGCTGAGCTC 
TTCCCAGATC CGAGTGTTGC TGTGGGCCCA GGGGGACCAA CGACGCCCAA GAAGCCACCT 
TCCCATCCTC CTCCCCACTA TCCAGGGGGG AAAAGGAAAA GAAGCTCTGC TCCACCTAGA 
AAGCTGCTGT CAGACAAGCC ACAGGACTTC CAGATCAGAG TCCAGGTGAT TGAGGGGCGT 
CAGCTGCCTG GGGTGAACAT CAAGCCTGTG GTCAAGGTCA CAGCTGCTGG GCAGACCAAG 
CGGACTCGAA TCCAGAAGGG AAATAGCCCA CTTTTCAATG AGACTCTTTT CTTCAACGTA 
TTTGACTCTC CCTTGGAACT GTTTGATGAG CCCATCTTCA TCACGGTGGT AGACTCACGC 
TCCCTCAGGA CAGATGCCCT CCTTGGCGAG TTCCGGATGG ATGTGGGCAC CGTGTACAGA 
GAGCCCCGAC ATGCTTATCT CCGGAAGTGG CTGCTGCTCT CCGATCCCGA TGACTTCTCT 
GCAGGGGCCA GAGGCTACCT CAAAGCCAGC CTGTGTGTGC TGGGGCCTGG GGACGAAGCT 
CCCCTAGACA AGAAAGACCC TTCTGAAGAC AAAGAGGACA TTGAAGGCAA CCTGCTTAGG 
CCCACTGGTG TGGCCCTTCG TGGTGCCCAT TTTTGCCTGA AGCTCTTCCG GGCTGAGGAC 
TTACCACAGA TGGACGATGC TGTGATGGAC AACGTCAAGC AAATCTTTGG CTTTGATAGC 
AACAAGAAGA ACCTGGTGGA CCCCTTTGTG GAAGTCAGCT TTGCTGGGAA AATGCTTTGC 
AGCAAGATCC TGGAGAAGAC TGCTAACCCT CAGTGGAACC AGAACATCAC ATTGCCTGCG 
ATGTTTCCCT CTATGTGTGA AAAAATGAGG ATTCGTGTCA TGGACTGGGA CCGCCTCACT 
CACAATGACA CTGTGGCTAC CACCTACTTG GGTATGTCGA AAATCTCTGC CACTGGAGGA 
GAAATAGAAG AGGAGCCTGC AGGTGTACTC AAGAGTCCAG CCACTGACCT GGATGACAAC 
CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG 
TTCACGGGCT TCCCAGATCC TTATGCGGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCA 
TATAGGGGCA GGCTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAGCACAG TGAACAGAAG 
GTGGAAGACC TTCCTGCTGA TGACATCTTG AGAGTGGAGA AGTACCTGCG GAGGCGCAAA 
TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATTGG GAACTACGGA AACAAGTTTG ACACCACCTG CTTGCCGTTG 
GCCTCTACCA CCCAATACAG CCGGGCAGTC TTTGATGGAT GCCACTACTA TTATTTGCCC 
TGGGGCAACG TGAAGCCTGT GGTAGTACTG TCCTCATACT GGGAAGACAT CAGCCATCGA 
GTCGAGACTC AGAACCAGCT CCTCAGGATC GCTGACCGAC TGGAAGCTAA CCTGGAGCAA 
GTCCACCTGG CTCTGAAGGC ACAGTGTTCC TCTGAGGATG TGGACGCCCT GGTGGCTCAG 
TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA TGAAATACCT 
TCTGCCACCC ACCTTGATCA GTACCTGTTC CGCCTCCGCT CCCGTCACCT GAGCCAGATC 
AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC CACAGTGAGC TCTCCACTGC CCTGGAGCAG 
GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA 
GATATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAACG GGTGCCTGCC 
CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAATCTG 
CAGACCATCT TTCTGAAATA TCCTATGGAA GGCATGCCTG GGCCCCGGAT GCCGGTGCAG 
ATCCGGATCA AGCTCTGGTT TGGGCTCTCA GTGGATGAGA AGGAATTCAA TCAGTTTGCA 
GAGGGGAAGC TCTCTGTCTT TGCTGAAACG TATGAGAACC AGACAAAGCT GGCGCTGGTC 
GGGAACTGGG GCACGACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC 
AAACTACCGA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT GCTCCATGAT GCTGACGCCG GTCATCTGAG CTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC TCGGCTACCT GGAGGCCAGT GGATCTATAT GAGTGACAAC 
TACACCGACG TGAATGGGGA GAAGGTGCTT CCAAAGGACG ATATTGAATG CCCGCTGGGT 
TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCCA AACACTGGGT CCCTGTTGAG 
AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CACTGAAGAA GCACAGGCAG GCAGAGGCTG AGGGCGAGGG CTGGGAATAC 
GCCTCTCTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACGG GGCCTGCAGC GGTGTTTGCC 
CTTGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGATTCCAT GTCTGTGTCT 
ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTGC GTTGCTACCT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAAGACTCC 
TTCTCTGATC CCTATGCCAT TGTCTCCTTC CTGCATCAAA GCCAGAAGAC GGTGGTGGAG 
AAGAACACCC TGAACCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT AGAGATCTTT 
GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA CCCAGCATTG TGGTGGAGCT GTATGACCAT 
GACACCTATG GCGCTGACGA GTTCATGGGC CGCTGTATTT GTCAGCCAAG CCTGGAACGG 
ATGCCCCGCC TGGCCTGGTT CCCACTGACC AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG 
GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG 
ATGCACGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGACTTGCC TTACCCACCA 
CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCAGC TCTCCAGCGT 
ACAGCCATCG AGATCCTGGC CTGGGGCCTT CGGAACATAA AGAGCTATCA GATGGCCAGC 
GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC 
AGAAACCTTC GGAAGAACCC CAACTTTGAC GTCTGTACTC TGTTCATGGA AGTGATGTTA 
CCCAGAGAGG ACCTCTACTG TCCCCCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT 
GGCCGCAGGC CAGTTGTGGG TCAGTGCACC ATTCGCTCCC TGGAGAACTT CCTGTGTGAC 
CCGTACTCAG CAGAGAGTCC ATCCCCGCAA GGTGGCCCAG ATGACGTGAG CTTACTCAGC 
CCTGGGGAAG ACGTGCTCAT TGACATTGAT GACAAGGAGC CTCTCATCCC TGTCCAGGAG 
GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTA TCGGGGAGAG AGAAAAGTGT 
GGTTCCTACC TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGGAGGCCT GTCCGACTTT TGTAATACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG ACACAGAAGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT 
TATCCCCTCC CAGAAGATCC AGCCATCCCT ATGCCCCCGA GACAATTCCA TCAGCTGGCT 
GCTCAGGGTC CCCAGGAATG CTTGGTCCGG ATTTACATCA TCCGAGCATT TGGCTTACAG 
CCCAAGGACC CCAATGGCAA GTGCGACCCT TACATCAAGA TCTCTATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCTTGT ACCCTGGAGC CTGTATTTGG AAAGATGTTT 
GAACTCACCT GCACTCTACC TCTGGAGAAG GACTTGAAGA TCACACTCTA TGACTATGAC 
CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACAGTCATTG ATCTGGAGAA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGCCTTCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG 
TGGAGAGATC AACTCCGCCC TTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGAATA CACCATTCAG 
GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CGGTGGAGGA ACGCTTAGCC 
CTGCATGTCC TTCAACAACA AGGCTTGGTC CCTGAGCATG TGGAGTCTCG GCCTCTGTAC 
AGTCCTCTGC AGCCAGATAT TGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA 
AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TCGAAGGTTT 
TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGCCTCACG 
GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAA 
CAGAAGACAG ATGTGCACTA CCGTTCCCTG GGCGGGGAGG GCAATTTCAA CTGGAGGTTC 
GTGTTTCCCT TCGACTATCT GCCCGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCT 
TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATTTGG 
GACAATGACA AGTTCTCCTT TGATGACTTT CTGGGCTCCC TGCAGCTGGA TCTCAACCGA 
ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT 
GTGACAGAGG AGGGCGCGAA GAAGATCCTG GCGGGGAAGC TGGAAATGAC CTTGGAGATC 
GTTGCAGAAA GTGAACATGA GGAGCGGCCT GCTGGCCAGG GTCGGGATGA ACCCAACATG 
AATCCAAAGC TAGAAGATCC CAGGCGCCCT GACACCTCTT TCCTGTGGTT CACCTCACCA 
TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGATTCCGGT GTGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101339.1
CDS	1..6090
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFREAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	NM_001107869.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107869.1

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GAAGCCAAGA TCCCACTCCA GGAGGTCCTT 
GCCACCCCCA GCCTATCTGC CAGCTTCAAC GCACCTCTGC TGGACTCCAA GCAGCAACCC 
ACAGGGGCCT CTCTGGTCCT GCAGGTATCC TACACACCGC CCCCAGGAGC TGCGCCCTTG 
TTCCCACCAC CCGCTTCCCT GGCACCGTCC CCAACACTGC CTGACATGGA CCTGGTGGCT 
GACACAGGAG GAGAGGAAGA CACGGAAGAC CAGGGCCTGA CTGGAGATGA AGCTGAGCTC 
TTCCCAGATC CGAGTGTTGC TGTGGGCCCA GGGGGACCAA CGACGCCCAA GAAGCCACCT 
TCCCATCCTC CTCCCCACTA TCCAGGGGGG AAAAGGAAAA GAAGCTCTGC TCCACCTAGA 
AAGCTGCTGT CAGACAAGCC ACAGGACTTC CAGATCAGAG TCCAGGTGAT TGAGGGGCGT 
CAGCTGCCTG GGGTGAACAT CAAGCCTGTG GTCAAGGTCA CAGCTGCTGG GCAGACCAAG 
CGGACTCGAA TCCAGAAGGG AAATAGCCCA CTTTTCAATG AGACTCTTTT CTTCAACGTA 
TTTGACTCTC CCTTGGAACT GTTTGATGAG CCCATCTTCA TCACGGTGGT AGACTCACGC 
TCCCTCAGGA CAGATGCCCT CCTTGGCGAG TTCCGGATGG ATGTGGGCAC CGTGTACAGA 
GAGCCCCGAC ATGCTTATCT CCGGAAGTGG CTGCTGCTCT CCGATCCCGA TGACTTCTCT 
GCAGGGGCCA GAGGCTACCT CAAAGCCAGC CTGTGTGTGC TGGGGCCTGG GGACGAAGCT 
CCCCTAGACA AGAAAGACCC TTCTGAAGAC AAAGAGGACA TTGAAGGCAA CCTGCTTAGG 
CCCACTGGTG TGGCCCTTCG TGGTGCCCAT TTTTGCCTGA AGCTCTTCCG GGCTGAGGAC 
TTACCACAGA TGGACGATGC TGTGATGGAC AACGTCAAGC AAATCTTTGG CTTTGATAGC 
AACAAGAAGA ACCTGGTGGA CCCCTTTGTG GAAGTCAGCT TTGCTGGGAA AATGCTTTGC 
AGCAAGATCC TGGAGAAGAC TGCTAACCCT CAGTGGAACC AGAACATCAC ATTGCCTGCG 
ATGTTTCCCT CTATGTGTGA AAAAATGAGG ATTCGTGTCA TGGACTGGGA CCGCCTCACT 
CACAATGACA CTGTGGCTAC CACCTACTTG GGTATGTCGA AAATCTCTGC CACTGGAGGA 
GAAATAGAAG AGGAGCCTGC AGGTGTACTC AAGAGTCCAG CCACTGACCT GGATGACAAC 
CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG 
TTCACGGGCT TCCCAGATCC TTATGCGGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCA 
TATAGGGGCA GGCTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAGCACAG TGAACAGAAG 
GTGGAAGACC TTCCTGCTGA TGACATCTTG AGAGTGGAGA AGTACCTGCG GAGGCGCAAA 
TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATTGG GAACTACGGA AACAAGTTTG ACACCACCTG CTTGCCGTTG 
GCCTCTACCA CCCAATACAG CCGGGCAGTC TTTGATGGAT GCCACTACTA TTATTTGCCC 
TGGGGCAACG TGAAGCCTGT GGTAGTACTG TCCTCATACT GGGAAGACAT CAGCCATCGA 
GTCGAGACTC AGAACCAGCT CCTCAGGATC GCTGACCGAC TGGAAGCTAA CCTGGAGCAA 
GTCCACCTGG CTCTGAAGGC ACAGTGTTCC TCTGAGGATG TGGACGCCCT GGTGGCTCAG 
TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA TGAAATACCT 
TCTGCCACCC ACCTTGATCA GTACCTGTTC CGCCTCCGCT CCCGTCACCT GAGCCAGATC 
AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC CACAGTGAGC TCTCCACTGC CCTGGAGCAG 
GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA 
GATATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAACG GGTGCCTGCC 
CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAATCTG 
CAGACCATCT TTCTGAAATA TCCTATGGAA GGCATGCCTG GGCCCCGGAT GCCGGTGCAG 
ATCCGGATCA AGCTCTGGTT TGGGCTCTCA GTGGATGAGA AGGAATTCAA TCAGTTTGCA 
GAGGGGAAGC TCTCTGTCTT TGCTGAAACG TATGAGAACC AGACAAAGCT GGCGCTGGTC 
GGGAACTGGG GCACGACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC 
AAACTACCGA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT GCTCCATGAT GCTGACGCCG GTCATCTGAG CTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC TCGGCTACCT GGAGGCCAGT GGATCTATAT GAGTGACAAC 
TACACCGACG TGAATGGGGA GAAGGTGCTT CCAAAGGACG ATATTGAATG CCCGCTGGGT 
TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCCA AACACTGGGT CCCTGTTGAG 
AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CACTGAAGAA GCACAGGCAG GCAGAGGCTG AGGGCGAGGG CTGGGAATAC 
GCCTCTCTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACGG GGCCTGCAGC GGTGTTTGCC 
CTTGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGATTCCAT GTCTGTGTCT 
ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTGC GTTGCTACCT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAAGACTCC 
TTCTCTGATC CCTATGCCAT TGTCTCCTTC CTGCATCAAA GCCAGAAGAC GGTGGTGGAG 
AAGAACACCC TGAACCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT AGAGATCTTT 
GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA CCCAGCATTG TGGTGGAGCT GTATGACCAT 
GACACCTATG GCGCTGACGA GTTCATGGGC CGCTGTATTT GTCAGCCAAG CCTGGAACGG 
ATGCCCCGCC TGGCCTGGTT CCCACTGACC AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG 
GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG 
ATGCACGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGACTTGCC TTACCCACCA 
CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCAGC TCTCCAGCGT 
ACAGCCATCG AGATCCTGGC CTGGGGCCTT CGGAACATAA AGAGCTATCA GATGGCCAGC 
GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC 
AGAAACCTTC GGAAGAACCC CAACTTTGAC GTCTGTACTC TGTTCATGGA AGTGATGTTA 
CCCAGAGAGG ACCTCTACTG TCCCCCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT 
GGCCGCAGGC CAGTTGTGGG TCAGTGCACC ATTCGCTCCC TGGAGAACTT CCTGTGTGAC 
CCGTACTCAG CAGAGAGTCC ATCCCCGCAA GGTGGCCCAG ATGACGTGAG CTTACTCAGC 
CCTGGGGAAG ACGTGCTCAT TGACATTGAT GACAAGGAGC CTCTCATCCC TGTCCAGGAG 
GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTA TCGGGGAGAG AGAAAAGTGT 
GGTTCCTACC TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGGAGGCCT GTCCGACTTT TGTAATACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG ACACAGAAGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT 
TATCCCCTCC CAGAAGATCC AGCCATCCCT ATGCCCCCGA GACAATTCCA TCAGCTGGCT 
GCTCAGGGTC CCCAGGAATG CTTGGTCCGG ATTTACATCA TCCGAGCATT TGGCTTACAG 
CCCAAGGACC CCAATGGCAA GTGCGACCCT TACATCAAGA TCTCTATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCTTGT ACCCTGGAGC CTGTATTTGG AAAGATGTTT 
GAACTCACCT GCACTCTACC TCTGGAGAAG GACTTGAAGA TCACACTCTA TGACTATGAC 
CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACAGTCATTG ATCTGGAGAA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGCCTTCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG 
TGGAGAGATC AACTCCGCCC TTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGAATA CACCATTCAG 
GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CGGTGGAGGA ACGCTTAGCC 
CTGCATGTCC TTCAACAACA AGGCTTGGTC CCTGAGCATG TGGAGTCTCG GCCTCTGTAC 
AGTCCTCTGC AGCCAGATAT TGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA 
AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TCGAAGGTTT 
TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGCCTCACG 
GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAA 
CAGAAGACAG ATGTGCACTA CCGTTCCCTG GGCGGGGAGG GCAATTTCAA CTGGAGGTTC 
GTGTTTCCCT TCGACTATCT GCCCGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCT 
TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATTTGG 
GACAATGACA AGTTCTCCTT TGATGACTTT CTGGGCTCCC TGCAGCTGGA TCTCAACCGA 
ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT 
GTGACAGAGG AGGGCGCGAA GAAGATCCTG GCGGGGAAGC TGGAAATGAC CTTGGAGATC 
GTTGCAGAAA GTGAACATGA GGAGCGGCCT GCTGGCCAGG GTCGGGATGA ACCCAACATG 
AATCCAAAGC TAGAAGATCC CAGGCGCCCT GACACCTCTT TCCTGTGGTT CACCTCACCA 
TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGATTCCGGT GTGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236758.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236820.1 Rattus norvegicus dysferlin (Dysf), transcript variant X4, mRNA.

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CloneID	ORa27399
Clone ID Related Accession (Same CDS sequence)	XM_006236758.3
Accession Version	XM_006236758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236758.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGACGGAGGA CACAGACTTG 
CCTTACCCAC CACCTCAAAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GCTCTCCAGC GTACAGCCAT CGAGATCCTG GCCTGGGGCC TTCGGAACAT AAAGAGCTAT 
CAGATGGCCA GCGTGTCTTC CCCCAGCCTC GTGGTGGAGT GTGGTGGGCA GACAGTGCAG 
TCCTGTGTCA TCAGAAACCT TCGGAAGAAC CCCAACTTTG ACGTCTGTAC TCTGTTCATG 
GAAGTGATGT TACCCAGAGA GGACCTCTAC TGTCCCCCTA TTGTGGTCAA GGTCATTGAT 
AATCGCCAGT TTGGCCGCAG GCCAGTTGTG GGTCAGTGCA CCATTCGCTC CCTGGAGAAC 
TTCCTGTGTG ACCCGTACTC AGCAGAGAGT CCATCCCCGC AAGGTGGCCC AGATGACGTG 
AGCTTACTCA GCCCTGGGGA AGACGTGCTC ATTGACATTG ATGACAAGGA GCCTCTCATC 
CCTGTCCAGC TTGCAGATGG GCTGTCAAGC TTGGGCCCCA CTAACCTGAT GCCTTCTCCG 
TCCAGTCCTC ATGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTATCGGG 
GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCCTGAA GGTCTATGAC 
ACACAGCTGG AGAATGTGGA GGCCTTTGGA GGCCTGTCCG ACTTTTGTAA TACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGACACA GAAGATCCAT CTGTCATCGG AGAATTTAAG 
GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCTATGCC CCCGAGACAA 
TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGGATTTA CATCATCCGA 
GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGCG ACCCTTACAT CAAGATCTCT 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGTACCCT GGAGCCTGTA 
TTTGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACTT GAAGATCACA 
CTCTATGACT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACAGT CATTGATCTG 
GAGAATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGCC TTCCACAGAC CTACTGTGTC 
TCTGGACCAA ATCAGTGGAG AGATCAACTC CGCCCTTCCC AGCTCCTCCA CCTCTTCTGC 
CAGCAGCACA GGGTCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG 
GAATACACCA TTCAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCGGTG 
GAGGAACGCT TAGCCCTGCA TGTCCTTCAA CAACAAGGCT TGGTCCCTGA GCATGTGGAG 
TCTCGGCCTC TGTACAGTCC TCTGCAGCCA GATATTGAGC AGGGGAAGCT ACAGATGTGG 
ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG 
AAAGCTCGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT 
GACCTCAGCC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA 
TTCGAAGAAC ACAAACAGAA GACAGATGTG CACTACCGTT CCCTGGGCGG GGAGGGCAAT 
TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCCG CTGAGCAAGT CTGTGCTGTT 
GCCAAGAAGG ATGCTTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG 
GTCTTCCAGA TTTGGGACAA TGACAAGTTC TCCTTTGATG ACTTTCTGGG CTCCCTGCAG 
CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA 
GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GCGAAGAAGA TCCTGGCGGG GAAGCTGGAA 
ATGACCTTGG AGATCGTTGC AGAAAGTGAA CATGAGGAGC GGCCTGCTGG CCAGGGTCGG 
GATGAACCCA ACATGAATCC AAAGCTAGAA GATCCCAGGC GCCCTGACAC CTCTTTCCTG 
TGGTTCACCT CACCATACAA GACCATGAAG TTCATCTTGT GGCGACGATT CCGGTGTGCC 
ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC 
TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTTA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236820.1
CDS	702..7106
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236758.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236758.3

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGACGGAGGA CACAGACTTG 
CCTTACCCAC CACCTCAAAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GCTCTCCAGC GTACAGCCAT CGAGATCCTG GCCTGGGGCC TTCGGAACAT AAAGAGCTAT 
CAGATGGCCA GCGTGTCTTC CCCCAGCCTC GTGGTGGAGT GTGGTGGGCA GACAGTGCAG 
TCCTGTGTCA TCAGAAACCT TCGGAAGAAC CCCAACTTTG ACGTCTGTAC TCTGTTCATG 
GAAGTGATGT TACCCAGAGA GGACCTCTAC TGTCCCCCTA TTGTGGTCAA GGTCATTGAT 
AATCGCCAGT TTGGCCGCAG GCCAGTTGTG GGTCAGTGCA CCATTCGCTC CCTGGAGAAC 
TTCCTGTGTG ACCCGTACTC AGCAGAGAGT CCATCCCCGC AAGGTGGCCC AGATGACGTG 
AGCTTACTCA GCCCTGGGGA AGACGTGCTC ATTGACATTG ATGACAAGGA GCCTCTCATC 
CCTGTCCAGC TTGCAGATGG GCTGTCAAGC TTGGGCCCCA CTAACCTGAT GCCTTCTCCG 
TCCAGTCCTC ATGAGGAGGA GTTCATTGAC TGGTGGAGCA AATTCTTTGC TTCTATCGGG 
GAGAGGGAAA AGTGTGGTTC CTACCTGGAG AAGGATTTTG ACACCCTGAA GGTCTATGAC 
ACACAGCTGG AGAATGTGGA GGCCTTTGGA GGCCTGTCCG ACTTTTGTAA TACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGACACA GAAGATCCAT CTGTCATCGG AGAATTTAAG 
GGTCTCTTCA AAATTTATCC CCTCCCAGAA GATCCAGCCA TCCCTATGCC CCCGAGACAA 
TTCCATCAGC TGGCTGCTCA GGGTCCCCAG GAATGCTTGG TCCGGATTTA CATCATCCGA 
GCATTTGGCT TACAGCCCAA GGACCCCAAT GGCAAGTGCG ACCCTTACAT CAAGATCTCT 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATCC CTTGTACCCT GGAGCCTGTA 
TTTGGAAAGA TGTTTGAACT CACCTGCACT CTACCTCTGG AGAAGGACTT GAAGATCACA 
CTCTATGACT ATGACCTGCT CTCCAAGGAT GAGAAGATTG GGGAGACAGT CATTGATCTG 
GAGAATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGTGGCC TTCCACAGAC CTACTGTGTC 
TCTGGACCAA ATCAGTGGAG AGATCAACTC CGCCCTTCCC AGCTCCTCCA CCTCTTCTGC 
CAGCAGCACA GGGTCAAGGC CCCCGTGTAC CGGACAGACC GAGTGACGTT TCAGGATAAG 
GAATACACCA TTCAGGAGAT AGAGGCTGGC AGACTCCCAA ACCCACACCT GGGCCCGGTG 
GAGGAACGCT TAGCCCTGCA TGTCCTTCAA CAACAAGGCT TGGTCCCTGA GCATGTGGAG 
TCTCGGCCTC TGTACAGTCC TCTGCAGCCA GATATTGAGC AGGGGAAGCT ACAGATGTGG 
ATTGACATAT TTCCAAAGGT GCTGGGCCGG CCTGGACCTC CCTTCAACAT CACCCCACGG 
AAAGCTCGAA GGTTTTTTCT GCGTTGTATT ATTTGGAACA CAAAAGATGT GATCTTGGAT 
GACCTCAGCC TCACGGGGGA GAAGATGAGT GACATCTATG TGAAAGGCTG GATGGTGGGA 
TTCGAAGAAC ACAAACAGAA GACAGATGTG CACTACCGTT CCCTGGGCGG GGAGGGCAAT 
TTCAACTGGA GGTTCGTGTT TCCCTTCGAC TATCTGCCCG CTGAGCAAGT CTGTGCTGTT 
GCCAAGAAGG ATGCTTTCTG GAGACTCGAC AAGACAGAGA GCAAGATCCC AGCACGTGTG 
GTCTTCCAGA TTTGGGACAA TGACAAGTTC TCCTTTGATG ACTTTCTGGG CTCCCTGCAG 
CTGGATCTCA ACCGAATGCC CAAGCCAGCC AAGACGGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CCTTCCACCC AGAATGGTTT GTGTCCCTTT TTGAGCAGAA GACAGTGAAA 
GGATGGTGGC CTTGTGTGAC AGAGGAGGGC GCGAAGAAGA TCCTGGCGGG GAAGCTGGAA 
ATGACCTTGG AGATCGTTGC AGAAAGTGAA CATGAGGAGC GGCCTGCTGG CCAGGGTCGG 
GATGAACCCA ACATGAATCC AAAGCTAGAA GATCCCAGGC GCCCTGACAC CTCTTTCCTG 
TGGTTCACCT CACCATACAA GACCATGAAG TTCATCTTGT GGCGACGATT CCGGTGTGCC 
ATCATCCTCT TCATCATCCT CTTCATCCTC CTGCTCTTCC TGGGTGTCTT TGTCTACGCC 
TTCCCGAACT ATGCTGCCAT GAAGCTGGTG AAGCCCTTTA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236760.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236822.1 Rattus norvegicus dysferlin (Dysf), transcript variant X6, mRNA.

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CloneID	ORa27401
Clone ID Related Accession (Same CDS sequence)	XM_006236760.3
Accession Version	XM_006236760.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236760.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGTGGATGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCG 
GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCATATAGGG GCAGGCTTCT GTTGTCCCTG 
GAGACCAAAC TGGTGGAGCA CAGTGAACAG AAGGTGGAAG ACCTTCCTGC TGATGACATC 
TTGAGAGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA 
GCCACAATGC TGCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC 
GGAAACAAGT TTGACACCAC CTGCTTGCCG TTGGCCTCTA CCACCCAATA CAGCCGGGCA 
GTCTTTGATG GATGCCACTA CTATTATTTG CCCTGGGGCA ACGTGAAGCC TGTGGTAGTA 
CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAGTCGAGA CTCAGAACCA GCTCCTCAGG 
ATCGCTGACC GACTGGAAGC TAACCTGGAG CAAGTCCACC TGGCTCTGAA GGCACAGTGT 
TCCTCTGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC 
AGCCAGCCCC TGTGTGACAT CCATGAAATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG 
TTCCGCCTCC GCTCCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCCCTGGC CTTGAAGCTT 
GGCCACAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT 
GCCCTGGCTG AGGAGCCCCA GAACAGCCTG CCAGATATCA TCATCTGGAT GCTGCAGGGT 
GACAAGCGTG TGGCCTACCA ACGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC 
CCCAGCTACT GCGGCAGGAA CTGTGGGAAT CTGCAGACCA TCTTTCTGAA ATATCCTATG 
GAAGGCATGC CTGGGCCCCG GATGCCGGTG CAGATCCGGA TCAAGCTCTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAATT CAATCAGTTT GCAGAGGGGA AGCTCTCTGT CTTTGCTGAA 
ACGTATGAGA ACCAGACAAA GCTGGCGCTG GTCGGGAACT GGGGCACGAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAACTAC CGAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC AGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GATGCTGACG CCGGTCATCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACTCGGCTA 
CCTGGAGGCC AGTGGATCTA TATGAGTGAC AACTACACCG ACGTGAATGG GGAGAAGGTG 
CTTCCAAAGG ACGATATTGA ATGCCCGCTG GGTTGGAAGT GGGAAGATGA AGAATGGTCC 
ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA 
GACCGAAAGC CCAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA 
CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AGGCACTGAA GAAGCACAGG 
CAGGCAGAGG CTGAGGGCGA GGGCTGGGAA TACGCCTCTC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA TGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCGGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCATGGTG 
GATGACAAGA GCGAAGATTC CATGTCTGTG TCTACCCTGA GCTTTGGCGT TAACAGACCC 
ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGTTGCTA CCTGTACCAG 
GCCCGGGACC TGCCCGCCAT GGACAAAGAC TCCTTCTCTG ATCCCTATGC CATTGTCTCC 
TTCCTGCATC AAAGCCAGAA GACGGTGGTG GAGAAGAACA CCCTGAACCC CACCTGGGAT 
CAAACCCTCA TCTTCTATGA GATAGAGATC TTTGGCGAGC CGGCCAGTGT CGCCGAGCGG 
CCACCCAGCA TTGTGGTGGA GCTGTATGAC CATGACACCT ATGGCGCTGA CGAGTTCATG 
GGCCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCTGC CGGAGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCACG AAACTTCAAG GATTTTGGAT 
GAGACGGAGG ACACAGACTT GCCTTACCCA CCACCTCAAA GGGAGGCCAA CATCTACATG 
GTTCCCCAGA ACATCAAGCC AGCTCTCCAG CGTACAGCCA TCGAGATCCT GGCCTGGGGC 
CTTCGGAACA TAAAGAGCTA TCAGATGGCC AGCGTGTCTT CCCCCAGCCT CGTGGTGGAG 
TGTGGTGGGC AGACAGTGCA GTCCTGTGTC ATCAGAAACC TTCGGAAGAA CCCCAACTTT 
GACGTCTGTA CTCTGTTCAT GGAAGTGATG TTACCCAGAG AGGACCTCTA CTGTCCCCCT 
ATTGTGGTCA AGGTCATTGA TAATCGCCAG TTTGGCCGCA GGCCAGTTGT GGGTCAGTGC 
ACCATTCGCT CCCTGGAGAA CTTCCTGTGT GACCCGTACT CAGCAGAGAG TCCATCCCCG 
CAAGGTGGCC CAGATGACGT GAGCTTACTC AGCCCTGGGG AAGACGTGCT CATTGACATT 
GATGACAAGG AGCCTCTCAT CCCTGTCCAG CTTGCAGATG GGCTGTCAAG CTTGGGCCCC 
ACTAACCTGA TGCCTTCTCC GTCCAGTCCT CATGAGGAGG AGTTCATTGA CTGGTGGAGC 
AAATTCTTTG CTTCTATCGG GGAGAGGGAA AAGTGTGGTT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGG AGGCCTGTCC 
GACTTTTGTA ATACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGACAC AGAAGATCCA 
TCTGTCATCG GAGAATTTAA GGGTCTCTTC AAAATTTATC CCCTCCCAGA AGATCCAGCC 
ATCCCTATGC CCCCGAGACA ATTCCATCAG CTGGCTGCTC AGGGTCCCCA GGAATGCTTG 
GTCCGGATTT ACATCATCCG AGCATTTGGC TTACAGCCCA AGGACCCCAA TGGCAAGTGC 
GACCCTTACA TCAAGATCTC TATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCTTGTACCC TGGAGCCTGT ATTTGGAAAG ATGTTTGAAC TCACCTGCAC TCTACCTCTG 
GAGAAGGACT TGAAGATCAC ACTCTATGAC TATGACCTGC TCTCCAAGGA TGAGAAGATT 
GGGGAGACAG TCATTGATCT GGAGAATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGC 
CTTCCACAGA CCTACTGTGT CTCTGGACCA AATCAGTGGA GAGATCAACT CCGCCCTTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC AGGGTCAAGG CCCCCGTGTA CCGGACAGAC 
CGAGTGACGT TTCAGGATAA GGAATACACC ATTCAGGAGA TAGAGGCTGG CAGACTCCCA 
AACCCACACC TGGGCCCGGT GGAGGAACGC TTAGCCCTGC ATGTCCTTCA ACAACAAGGC 
TTGGTCCCTG AGCATGTGGA GTCTCGGCCT CTGTACAGTC CTCTGCAGCC AGATATTGAG 
CAGGGGAAGC TACAGATGTG GATTGACATA TTTCCAAAGG TGCTGGGCCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAAAGCTCGA AGGTTTTTTC TGCGTTGTAT TATTTGGAAC 
ACAAAAGATG TGATCTTGGA TGACCTCAGC CTCACGGGGG AGAAGATGAG TGACATCTAT 
GTGAAAGGCT GGATGGTGGG ATTCGAAGAA CACAAACAGA AGACAGATGT GCACTACCGT 
TCCCTGGGCG GGGAGGGCAA TTTCAACTGG AGGTTCGTGT TTCCCTTCGA CTATCTGCCC 
GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG GATGCTTTCT GGAGACTCGA CAAGACAGAG 
AGCAAGATCC CAGCACGTGT GGTCTTCCAG ATTTGGGACA ATGACAAGTT CTCCTTTGAT 
GACTTTCTGG GCTCCCTGCA GCTGGATCTC AACCGAATGC CCAAGCCAGC CAAGACGGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACCTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AGACAGTGAA AGGATGGTGG CCTTGTGTGA CAGAGGAGGG CGCGAAGAAG 
ATCCTGGCGG GGAAGCTGGA AATGACCTTG GAGATCGTTG CAGAAAGTGA ACATGAGGAG 
CGGCCTGCTG GCCAGGGTCG GGATGAACCC AACATGAATC CAAAGCTAGA AGATCCCAGG 
CGCCCTGACA CCTCTTTCCT GTGGTTCACC TCACCATACA AGACCATGAA GTTCATCTTG 
TGGCGACGAT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT CCTGCTCTTC 
CTGGGTGTCT TTGTCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236822.1
CDS	702..7067
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236760.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236760.3

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGTGGATGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCG 
GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCATATAGGG GCAGGCTTCT GTTGTCCCTG 
GAGACCAAAC TGGTGGAGCA CAGTGAACAG AAGGTGGAAG ACCTTCCTGC TGATGACATC 
TTGAGAGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA 
GCCACAATGC TGCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC 
GGAAACAAGT TTGACACCAC CTGCTTGCCG TTGGCCTCTA CCACCCAATA CAGCCGGGCA 
GTCTTTGATG GATGCCACTA CTATTATTTG CCCTGGGGCA ACGTGAAGCC TGTGGTAGTA 
CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAGTCGAGA CTCAGAACCA GCTCCTCAGG 
ATCGCTGACC GACTGGAAGC TAACCTGGAG CAAGTCCACC TGGCTCTGAA GGCACAGTGT 
TCCTCTGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC 
AGCCAGCCCC TGTGTGACAT CCATGAAATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG 
TTCCGCCTCC GCTCCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCCCTGGC CTTGAAGCTT 
GGCCACAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT 
GCCCTGGCTG AGGAGCCCCA GAACAGCCTG CCAGATATCA TCATCTGGAT GCTGCAGGGT 
GACAAGCGTG TGGCCTACCA ACGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC 
CCCAGCTACT GCGGCAGGAA CTGTGGGAAT CTGCAGACCA TCTTTCTGAA ATATCCTATG 
GAAGGCATGC CTGGGCCCCG GATGCCGGTG CAGATCCGGA TCAAGCTCTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAATT CAATCAGTTT GCAGAGGGGA AGCTCTCTGT CTTTGCTGAA 
ACGTATGAGA ACCAGACAAA GCTGGCGCTG GTCGGGAACT GGGGCACGAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAACTAC CGAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC AGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GATGCTGACG CCGGTCATCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACTCGGCTA 
CCTGGAGGCC AGTGGATCTA TATGAGTGAC AACTACACCG ACGTGAATGG GGAGAAGGTG 
CTTCCAAAGG ACGATATTGA ATGCCCGCTG GGTTGGAAGT GGGAAGATGA AGAATGGTCC 
ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA 
GACCGAAAGC CCAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA 
CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AGGCACTGAA GAAGCACAGG 
CAGGCAGAGG CTGAGGGCGA GGGCTGGGAA TACGCCTCTC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA TGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCGGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCATGGTG 
GATGACAAGA GCGAAGATTC CATGTCTGTG TCTACCCTGA GCTTTGGCGT TAACAGACCC 
ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGTTGCTA CCTGTACCAG 
GCCCGGGACC TGCCCGCCAT GGACAAAGAC TCCTTCTCTG ATCCCTATGC CATTGTCTCC 
TTCCTGCATC AAAGCCAGAA GACGGTGGTG GAGAAGAACA CCCTGAACCC CACCTGGGAT 
CAAACCCTCA TCTTCTATGA GATAGAGATC TTTGGCGAGC CGGCCAGTGT CGCCGAGCGG 
CCACCCAGCA TTGTGGTGGA GCTGTATGAC CATGACACCT ATGGCGCTGA CGAGTTCATG 
GGCCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCTGC CGGAGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCACG AAACTTCAAG GATTTTGGAT 
GAGACGGAGG ACACAGACTT GCCTTACCCA CCACCTCAAA GGGAGGCCAA CATCTACATG 
GTTCCCCAGA ACATCAAGCC AGCTCTCCAG CGTACAGCCA TCGAGATCCT GGCCTGGGGC 
CTTCGGAACA TAAAGAGCTA TCAGATGGCC AGCGTGTCTT CCCCCAGCCT CGTGGTGGAG 
TGTGGTGGGC AGACAGTGCA GTCCTGTGTC ATCAGAAACC TTCGGAAGAA CCCCAACTTT 
GACGTCTGTA CTCTGTTCAT GGAAGTGATG TTACCCAGAG AGGACCTCTA CTGTCCCCCT 
ATTGTGGTCA AGGTCATTGA TAATCGCCAG TTTGGCCGCA GGCCAGTTGT GGGTCAGTGC 
ACCATTCGCT CCCTGGAGAA CTTCCTGTGT GACCCGTACT CAGCAGAGAG TCCATCCCCG 
CAAGGTGGCC CAGATGACGT GAGCTTACTC AGCCCTGGGG AAGACGTGCT CATTGACATT 
GATGACAAGG AGCCTCTCAT CCCTGTCCAG CTTGCAGATG GGCTGTCAAG CTTGGGCCCC 
ACTAACCTGA TGCCTTCTCC GTCCAGTCCT CATGAGGAGG AGTTCATTGA CTGGTGGAGC 
AAATTCTTTG CTTCTATCGG GGAGAGGGAA AAGTGTGGTT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGG AGGCCTGTCC 
GACTTTTGTA ATACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGACAC AGAAGATCCA 
TCTGTCATCG GAGAATTTAA GGGTCTCTTC AAAATTTATC CCCTCCCAGA AGATCCAGCC 
ATCCCTATGC CCCCGAGACA ATTCCATCAG CTGGCTGCTC AGGGTCCCCA GGAATGCTTG 
GTCCGGATTT ACATCATCCG AGCATTTGGC TTACAGCCCA AGGACCCCAA TGGCAAGTGC 
GACCCTTACA TCAAGATCTC TATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCTTGTACCC TGGAGCCTGT ATTTGGAAAG ATGTTTGAAC TCACCTGCAC TCTACCTCTG 
GAGAAGGACT TGAAGATCAC ACTCTATGAC TATGACCTGC TCTCCAAGGA TGAGAAGATT 
GGGGAGACAG TCATTGATCT GGAGAATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGC 
CTTCCACAGA CCTACTGTGT CTCTGGACCA AATCAGTGGA GAGATCAACT CCGCCCTTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC AGGGTCAAGG CCCCCGTGTA CCGGACAGAC 
CGAGTGACGT TTCAGGATAA GGAATACACC ATTCAGGAGA TAGAGGCTGG CAGACTCCCA 
AACCCACACC TGGGCCCGGT GGAGGAACGC TTAGCCCTGC ATGTCCTTCA ACAACAAGGC 
TTGGTCCCTG AGCATGTGGA GTCTCGGCCT CTGTACAGTC CTCTGCAGCC AGATATTGAG 
CAGGGGAAGC TACAGATGTG GATTGACATA TTTCCAAAGG TGCTGGGCCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAAAGCTCGA AGGTTTTTTC TGCGTTGTAT TATTTGGAAC 
ACAAAAGATG TGATCTTGGA TGACCTCAGC CTCACGGGGG AGAAGATGAG TGACATCTAT 
GTGAAAGGCT GGATGGTGGG ATTCGAAGAA CACAAACAGA AGACAGATGT GCACTACCGT 
TCCCTGGGCG GGGAGGGCAA TTTCAACTGG AGGTTCGTGT TTCCCTTCGA CTATCTGCCC 
GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG GATGCTTTCT GGAGACTCGA CAAGACAGAG 
AGCAAGATCC CAGCACGTGT GGTCTTCCAG ATTTGGGACA ATGACAAGTT CTCCTTTGAT 
GACTTTCTGG GCTCCCTGCA GCTGGATCTC AACCGAATGC CCAAGCCAGC CAAGACGGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACCTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AGACAGTGAA AGGATGGTGG CCTTGTGTGA CAGAGGAGGG CGCGAAGAAG 
ATCCTGGCGG GGAAGCTGGA AATGACCTTG GAGATCGTTG CAGAAAGTGA ACATGAGGAG 
CGGCCTGCTG GCCAGGGTCG GGATGAACCC AACATGAATC CAAAGCTAGA AGATCCCAGG 
CGCCCTGACA CCTCTTTCCT GTGGTTCACC TCACCATACA AGACCATGAA GTTCATCTTG 
TGGCGACGAT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT CCTGCTCTTC 
CTGGGTGTCT TTGTCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236761.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236823.1 Rattus norvegicus dysferlin (Dysf), transcript variant X7, mRNA.

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CloneID	ORa27402
Clone ID Related Accession (Same CDS sequence)	XM_006236761.3
Accession Version	XM_006236761.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236761.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ATTCTGGGAA GAAGTGGCCA 
GTCCCCACGG ACACAGGAGG AGAGGAAGAC ACGGAAGACC AGGGCCTGAC TGGAGATGAA 
GCTGAGCTCT TCCCAGATCC GAGTGTTGCT GTGGGCCCAG GGGGACCAAC GACGCCCAAG 
AAGCCACCTT CCCATCCTCC TCCCCACTAT CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT 
CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATT 
GAGGGGCGTC AGCTGCCTGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG 
CAGACCAAGC GGACTCGAAT CCAGAAGGGA AATAGCCCAC TTTTCAATGA GACTCTTTTC 
TTCAACGTAT TTGACTCTCC CTTGGAACTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCACGCT CCCTCAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC 
GTGTACAGAG AGCCCCGACA TGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGATCCCGAT 
GACTTCTCTG CAGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG 
GACGAAGCTC CCCTAGACAA GAAAGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC 
CTGCTTAGGC CCACTGGTGT GGCCCTTCGT GGTGCCCATT TTTGCCTGAA GCTCTTCCGG 
GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA AATCTTTGGC 
TTTGATAGCA ACAAGAAGAA CCTGGTGGAC CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA 
ATGCTTTGCA GCAAGATCCT GGAGAAGACT GCTAACCCTC AGTGGAACCA GAACATCACA 
TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACTC ACAATGACAC TGTGGCTACC ACCTACTTGG GTATGTCGAA AATCTCTGCC 
ACTGGAGGAG AAATAGAAGT GGATGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC 
TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCGGAG 
CTCAACACAG GAAAGGGAGA AGGTGTGGCA TATAGGGGCA GGCTTCTGTT GTCCCTGGAG 
ACCAAACTGG TGGAGCACAG TGAACAGAAG GTGGAAGACC TTCCTGCTGA TGACATCTTG 
AGAGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC 
ACAATGCTGC AGGATGTGGA TGATGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGA 
AACAAGTTTG ACACCACCTG CTTGCCGTTG GCCTCTACCA CCCAATACAG CCGGGCAGTC 
TTTGATGGAT GCCACTACTA TTATTTGCCC TGGGGCAACG TGAAGCCTGT GGTAGTACTG 
TCCTCATACT GGGAAGACAT CAGCCATCGA GTCGAGACTC AGAACCAGCT CCTCAGGATC 
GCTGACCGAC TGGAAGCTAA CCTGGAGCAA GTCCACCTGG CTCTGAAGGC ACAGTGTTCC 
TCTGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC 
CAGCCCCTGT GTGACATCCA TGAAATACCT TCTGCCACCC ACCTTGATCA GTACCTGTTC 
CGCCTCCGCT CCCGTCACCT GAGCCAGATC AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC 
CACAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC 
CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA GATATCATCA TCTGGATGCT GCAGGGTGAC 
AAGCGTGTGG CCTACCAACG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC 
AGCTACTGCG GCAGGAACTG TGGGAATCTG CAGACCATCT TTCTGAAATA TCCTATGGAA 
GGCATGCCTG GGCCCCGGAT GCCGGTGCAG ATCCGGATCA AGCTCTGGTT TGGGCTCTCA 
GTGGATGAGA AGGAATTCAA TCAGTTTGCA GAGGGGAAGC TCTCTGTCTT TGCTGAAACG 
TATGAGAACC AGACAAAGCT GGCGCTGGTC GGGAACTGGG GCACGACGGG CCTCACCTAC 
CCCAAGTTTT CTGACGTCAC AGGCAAGATC AAACTACCGA AGGATAGCTT CCGCCCCTCA 
GCTGGCTGGG CCTGGGCAGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAT 
GCTGACGCCG GTCATCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC TCGGCTACCT 
GGAGGCCAGT GGATCTATAT GAGTGACAAC TACACCGACG TGAATGGGGA GAAGGTGCTT 
CCAAAGGACG ATATTGAATG CCCGCTGGGT TGGAAGTGGG AAGATGAAGA ATGGTCCACA 
GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC 
CGAAAGCCCA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC 
TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAA GCACAGGCAG 
GCAGAGGCTG AGGGCGAGGG CTGGGAATAC GCCTCTCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACAGATGC CTTTCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCACTG 
GAGAAGACGG GGCCTGCAGC GGTGTTTGCC CTTGAGGGGG CCCTGGGCGG CATGGTGGAT 
GACAAGAGCG AAGATTCCAT GTCTGTGTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA 
ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GTTGCTACCT GTACCAGGCC 
CGGGACCTGC CCGCCATGGA CAAAGACTCC TTCTCTGATC CCTATGCCAT TGTCTCCTTC 
CTGCATCAAA GCCAGAAGAC GGTGGTGGAG AAGAACACCC TGAACCCCAC CTGGGATCAA 
ACCCTCATCT TCTATGAGAT AGAGATCTTT GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTATG GCGCTGACGA GTTCATGGGC 
CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC 
AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG 
CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCACGAAA CTTCAAGGAT TTTGGATGAG 
ACGGAGGACA CAGACTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCAGC TCTCCAGCGT ACAGCCATCG AGATCCTGGC CTGGGGCCTT 
CGGAACATAA AGAGCTATCA GATGGCCAGC GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT 
GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAC 
GTCTGTACTC TGTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCCCCTATT 
GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCAGGC CAGTTGTGGG TCAGTGCACC 
ATTCGCTCCC TGGAGAACTT CCTGTGTGAC CCGTACTCAG CAGAGAGTCC ATCCCCGCAA 
GGTGGCCCAG ATGACGTGAG CTTACTCAGC CCTGGGGAAG ACGTGCTCAT TGACATTGAT 
GACAAGGAGC CTCTCATCCC TGTCCAGCTT GCAGATGGGC TGTCAAGCTT GGGCCCCACT 
AACCTGATGC CTTCTCCGTC CAGTCCTCAT GAGGAGGAGT TCATTGACTG GTGGAGCAAA 
TTCTTTGCTT CTATCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGGAGG CCTGTCCGAC 
TTTTGTAATA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGACACAGA AGATCCATCT 
GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC 
CCTATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC 
CGGATTTACA TCATCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGCGAC 
CCTTACATCA AGATCTCTAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT 
TGTACCCTGG AGCCTGTATT TGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG 
AAGGACTTGA AGATCACACT CTATGACTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG 
GAGACAGTCA TTGATCTGGA GAATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGCCTT 
CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAACTCCG CCCTTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCACAGG GTCAAGGCCC CCGTGTACCG GACAGACCGA 
GTGACGTTTC AGGATAAGGA ATACACCATT CAGGAGATAG AGGCTGGCAG ACTCCCAAAC 
CCACACCTGG GCCCGGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAACA ACAAGGCTTG 
GTCCCTGAGC ATGTGGAGTC TCGGCCTCTG TACAGTCCTC TGCAGCCAGA TATTGAGCAG 
GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAA AGCTCGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA 
AAAGATGTGA TCTTGGATGA CCTCAGCCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG 
AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAACAGAAGA CAGATGTGCA CTACCGTTCC 
CTGGGCGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCCGCT 
GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCTTTCTGGA GACTCGACAA GACAGAGAGC 
AAGATCCCAG CACGTGTGGT CTTCCAGATT TGGGACAATG ACAAGTTCTC CTTTGATGAC 
TTTCTGGGCT CCCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGC GAAGAAGATC 
CTGGCGGGGA AGCTGGAAAT GACCTTGGAG ATCGTTGCAG AAAGTGAACA TGAGGAGCGG 
CCTGCTGGCC AGGGTCGGGA TGAACCCAAC ATGAATCCAA AGCTAGAAGA TCCCAGGCGC 
CCTGACACCT CTTTCCTGTG GTTCACCTCA CCATACAAGA CCATGAAGTT CATCTTGTGG 
CGACGATTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG 
GGTGTCTTTG TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTTAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236823.1
CDS	399..6761
Translation	MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236761.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236761.3

ORF Insert Sequence:

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ATTCTGGGAA GAAGTGGCCA 
GTCCCCACGG ACACAGGAGG AGAGGAAGAC ACGGAAGACC AGGGCCTGAC TGGAGATGAA 
GCTGAGCTCT TCCCAGATCC GAGTGTTGCT GTGGGCCCAG GGGGACCAAC GACGCCCAAG 
AAGCCACCTT CCCATCCTCC TCCCCACTAT CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT 
CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATT 
GAGGGGCGTC AGCTGCCTGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG 
CAGACCAAGC GGACTCGAAT CCAGAAGGGA AATAGCCCAC TTTTCAATGA GACTCTTTTC 
TTCAACGTAT TTGACTCTCC CTTGGAACTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCACGCT CCCTCAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC 
GTGTACAGAG AGCCCCGACA TGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGATCCCGAT 
GACTTCTCTG CAGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG 
GACGAAGCTC CCCTAGACAA GAAAGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC 
CTGCTTAGGC CCACTGGTGT GGCCCTTCGT GGTGCCCATT TTTGCCTGAA GCTCTTCCGG 
GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA AATCTTTGGC 
TTTGATAGCA ACAAGAAGAA CCTGGTGGAC CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA 
ATGCTTTGCA GCAAGATCCT GGAGAAGACT GCTAACCCTC AGTGGAACCA GAACATCACA 
TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACTC ACAATGACAC TGTGGCTACC ACCTACTTGG GTATGTCGAA AATCTCTGCC 
ACTGGAGGAG AAATAGAAGT GGATGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC 
TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCGGAG 
CTCAACACAG GAAAGGGAGA AGGTGTGGCA TATAGGGGCA GGCTTCTGTT GTCCCTGGAG 
ACCAAACTGG TGGAGCACAG TGAACAGAAG GTGGAAGACC TTCCTGCTGA TGACATCTTG 
AGAGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC 
ACAATGCTGC AGGATGTGGA TGATGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGA 
AACAAGTTTG ACACCACCTG CTTGCCGTTG GCCTCTACCA CCCAATACAG CCGGGCAGTC 
TTTGATGGAT GCCACTACTA TTATTTGCCC TGGGGCAACG TGAAGCCTGT GGTAGTACTG 
TCCTCATACT GGGAAGACAT CAGCCATCGA GTCGAGACTC AGAACCAGCT CCTCAGGATC 
GCTGACCGAC TGGAAGCTAA CCTGGAGCAA GTCCACCTGG CTCTGAAGGC ACAGTGTTCC 
TCTGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC 
CAGCCCCTGT GTGACATCCA TGAAATACCT TCTGCCACCC ACCTTGATCA GTACCTGTTC 
CGCCTCCGCT CCCGTCACCT GAGCCAGATC AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC 
CACAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC 
CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA GATATCATCA TCTGGATGCT GCAGGGTGAC 
AAGCGTGTGG CCTACCAACG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC 
AGCTACTGCG GCAGGAACTG TGGGAATCTG CAGACCATCT TTCTGAAATA TCCTATGGAA 
GGCATGCCTG GGCCCCGGAT GCCGGTGCAG ATCCGGATCA AGCTCTGGTT TGGGCTCTCA 
GTGGATGAGA AGGAATTCAA TCAGTTTGCA GAGGGGAAGC TCTCTGTCTT TGCTGAAACG 
TATGAGAACC AGACAAAGCT GGCGCTGGTC GGGAACTGGG GCACGACGGG CCTCACCTAC 
CCCAAGTTTT CTGACGTCAC AGGCAAGATC AAACTACCGA AGGATAGCTT CCGCCCCTCA 
GCTGGCTGGG CCTGGGCAGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAT 
GCTGACGCCG GTCATCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC TCGGCTACCT 
GGAGGCCAGT GGATCTATAT GAGTGACAAC TACACCGACG TGAATGGGGA GAAGGTGCTT 
CCAAAGGACG ATATTGAATG CCCGCTGGGT TGGAAGTGGG AAGATGAAGA ATGGTCCACA 
GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC 
CGAAAGCCCA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC 
TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAA GCACAGGCAG 
GCAGAGGCTG AGGGCGAGGG CTGGGAATAC GCCTCTCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACAGATGC CTTTCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCACTG 
GAGAAGACGG GGCCTGCAGC GGTGTTTGCC CTTGAGGGGG CCCTGGGCGG CATGGTGGAT 
GACAAGAGCG AAGATTCCAT GTCTGTGTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA 
ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GTTGCTACCT GTACCAGGCC 
CGGGACCTGC CCGCCATGGA CAAAGACTCC TTCTCTGATC CCTATGCCAT TGTCTCCTTC 
CTGCATCAAA GCCAGAAGAC GGTGGTGGAG AAGAACACCC TGAACCCCAC CTGGGATCAA 
ACCCTCATCT TCTATGAGAT AGAGATCTTT GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTATG GCGCTGACGA GTTCATGGGC 
CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC 
AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG 
CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCACGAAA CTTCAAGGAT TTTGGATGAG 
ACGGAGGACA CAGACTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCAGC TCTCCAGCGT ACAGCCATCG AGATCCTGGC CTGGGGCCTT 
CGGAACATAA AGAGCTATCA GATGGCCAGC GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT 
GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAC 
GTCTGTACTC TGTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCCCCTATT 
GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCAGGC CAGTTGTGGG TCAGTGCACC 
ATTCGCTCCC TGGAGAACTT CCTGTGTGAC CCGTACTCAG CAGAGAGTCC ATCCCCGCAA 
GGTGGCCCAG ATGACGTGAG CTTACTCAGC CCTGGGGAAG ACGTGCTCAT TGACATTGAT 
GACAAGGAGC CTCTCATCCC TGTCCAGCTT GCAGATGGGC TGTCAAGCTT GGGCCCCACT 
AACCTGATGC CTTCTCCGTC CAGTCCTCAT GAGGAGGAGT TCATTGACTG GTGGAGCAAA 
TTCTTTGCTT CTATCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGGAGG CCTGTCCGAC 
TTTTGTAATA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGACACAGA AGATCCATCT 
GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC 
CCTATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC 
CGGATTTACA TCATCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGCGAC 
CCTTACATCA AGATCTCTAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT 
TGTACCCTGG AGCCTGTATT TGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG 
AAGGACTTGA AGATCACACT CTATGACTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG 
GAGACAGTCA TTGATCTGGA GAATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGCCTT 
CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAACTCCG CCCTTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCACAGG GTCAAGGCCC CCGTGTACCG GACAGACCGA 
GTGACGTTTC AGGATAAGGA ATACACCATT CAGGAGATAG AGGCTGGCAG ACTCCCAAAC 
CCACACCTGG GCCCGGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAACA ACAAGGCTTG 
GTCCCTGAGC ATGTGGAGTC TCGGCCTCTG TACAGTCCTC TGCAGCCAGA TATTGAGCAG 
GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAA AGCTCGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA 
AAAGATGTGA TCTTGGATGA CCTCAGCCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG 
AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAACAGAAGA CAGATGTGCA CTACCGTTCC 
CTGGGCGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCCGCT 
GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCTTTCTGGA GACTCGACAA GACAGAGAGC 
AAGATCCCAG CACGTGTGGT CTTCCAGATT TGGGACAATG ACAAGTTCTC CTTTGATGAC 
TTTCTGGGCT CCCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGC GAAGAAGATC 
CTGGCGGGGA AGCTGGAAAT GACCTTGGAG ATCGTTGCAG AAAGTGAACA TGAGGAGCGG 
CCTGCTGGCC AGGGTCGGGA TGAACCCAAC ATGAATCCAA AGCTAGAAGA TCCCAGGCGC 
CCTGACACCT CTTTCCTGTG GTTCACCTCA CCATACAAGA CCATGAAGTT CATCTTGTGG 
CGACGATTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG 
GGTGTCTTTG TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTTAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236764.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236826.1 Rattus norvegicus dysferlin (Dysf), transcript variant X10, mRNA.

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CloneID	ORa27405
Clone ID Related Accession (Same CDS sequence)	XM_006236764.3
Accession Version	XM_006236764.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGACGGAGGA CACAGACTTG 
CCTTACCCAC CACCTCAAAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GCTCTCCAGC GTACAGCCAT CGAGATCCTG GCCTGGGGCC TTCGGAACAT AAAGAGCTAT 
CAGATGGCCA GCGTGTCTTC CCCCAGCCTC GTGGTGGAGT GTGGTGGGCA GACAGTGCAG 
TCCTGTGTCA TCAGAAACCT TCGGAAGAAC CCCAACTTTG ACGTCTGTAC TCTGTTCATG 
GAAGTGATGT TACCCAGAGA GGACCTCTAC TGTCCCCCTA TTGTGGTCAA GGTCATTGAT 
AATCGCCAGT TTGGCCGCAG GCCAGTTGTG GGTCAGTGCA CCATTCGCTC CCTGGAGAAC 
TTCCTGTGTG ACCCGTACTC AGCAGAGAGT CCATCCCCGC AAGGTGGCCC AGATGACGTG 
AGCTTACTCA GCCCTGGGGA AGACGTGCTC ATTGACATTG ATGACAAGGA GCCTCTCATC 
CCTGTCCAGG AGGAGGAGTT CATTGACTGG TGGAGCAAAT TCTTTGCTTC TATCGGGGAG 
AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGGAGGC CTGTCCGACT TTTGTAATAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGACACAGAA GATCCATCTG TCATCGGAGA ATTTAAGGGT 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAT CCAGCCATCC CTATGCCCCC GAGACAATTC 
CATCAGCTGG CTGCTCAGGG TCCCCAGGAA TGCTTGGTCC GGATTTACAT CATCCGAGCA 
TTTGGCTTAC AGCCCAAGGA CCCCAATGGC AAGTGCGACC CTTACATCAA GATCTCTATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCTT GTACCCTGGA GCCTGTATTT 
GGAAAGATGT TTGAACTCAC CTGCACTCTA CCTCTGGAGA AGGACTTGAA GATCACACTC 
TATGACTATG ACCTGCTCTC CAAGGATGAG AAGATTGGGG AGACAGTCAT TGATCTGGAG 
AATAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGCCTTC CACAGACCTA CTGTGTCTCT 
GGACCAAATC AGTGGAGAGA TCAACTCCGC CCTTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCACAGGG TCAAGGCCCC CGTGTACCGG ACAGACCGAG TGACGTTTCA GGATAAGGAA 
TACACCATTC AGGAGATAGA GGCTGGCAGA CTCCCAAACC CACACCTGGG CCCGGTGGAG 
GAACGCTTAG CCCTGCATGT CCTTCAACAA CAAGGCTTGG TCCCTGAGCA TGTGGAGTCT 
CGGCCTCTGT ACAGTCCTCT GCAGCCAGAT ATTGAGCAGG GGAAGCTACA GATGTGGATT 
GACATATTTC CAAAGGTGCT GGGCCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAAA 
GCTCGAAGGT TTTTTCTGCG TTGTATTATT TGGAACACAA AAGATGTGAT CTTGGATGAC 
CTCAGCCTCA CGGGGGAGAA GATGAGTGAC ATCTATGTGA AAGGCTGGAT GGTGGGATTC 
GAAGAACACA AACAGAAGAC AGATGTGCAC TACCGTTCCC TGGGCGGGGA GGGCAATTTC 
AACTGGAGGT TCGTGTTTCC CTTCGACTAT CTGCCCGCTG AGCAAGTCTG TGCTGTTGCC 
AAGAAGGATG CTTTCTGGAG ACTCGACAAG ACAGAGAGCA AGATCCCAGC ACGTGTGGTC 
TTCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGACT TTCTGGGCTC CCTGCAGCTG 
GATCTCAACC GAATGCCCAA GCCAGCCAAG ACGGCTGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACCT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA 
TGGTGGCCTT GTGTGACAGA GGAGGGCGCG AAGAAGATCC TGGCGGGGAA GCTGGAAATG 
ACCTTGGAGA TCGTTGCAGA AAGTGAACAT GAGGAGCGGC CTGCTGGCCA GGGTCGGGAT 
GAACCCAACA TGAATCCAAA GCTAGAAGAT CCCAGGCGCC CTGACACCTC TTTCCTGTGG 
TTCACCTCAC CATACAAGAC CATGAAGTTC ATCTTGTGGC GACGATTCCG GTGTGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTCCTG CTCTTCCTGG GTGTCTTTGT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTTAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236826.1
CDS	702..7043
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236764.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236764.3

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTAC TCAAGAGTCC AGCCACTGAC 
CTGGATGACA ACCTGGGCTT CCTCCCCACT TTTGGGCCCT GCTATGTGAA CCTCTATGGC 
AGTCCCCGAG AGTTCACGGG CTTCCCAGAT CCTTATGCGG AGCTCAACAC AGGAAAGGGA 
GAAGGTGTGG CATATAGGGG CAGGCTTCTG TTGTCCCTGG AGACCAAACT GGTGGAGCAC 
AGTGAACAGA AGGTGGAAGA CCTTCCTGCT GATGACATCT TGAGAGTGGA GAAGTACCTG 
CGGAGGCGCA AATATTCCCT CTTTGCTGCC TTCTACTCAG CCACAATGCT GCAGGATGTG 
GATGATGCCA TCCAGTTCGA GGTCAGCATT GGGAACTACG GAAACAAGTT TGACACCACC 
TGCTTGCCGT TGGCCTCTAC CACCCAATAC AGCCGGGCAG TCTTTGATGG ATGCCACTAC 
TATTATTTGC CCTGGGGCAA CGTGAAGCCT GTGGTAGTAC TGTCCTCATA CTGGGAAGAC 
ATCAGCCATC GAGTCGAGAC TCAGAACCAG CTCCTCAGGA TCGCTGACCG ACTGGAAGCT 
AACCTGGAGC AAGTCCACCT GGCTCTGAAG GCACAGTGTT CCTCTGAGGA TGTGGACGCC 
CTGGTGGCTC AGTTGACAGA TGAGCTCCTT GCAGACTGCA GCCAGCCCCT GTGTGACATC 
CATGAAATAC CTTCTGCCAC CCACCTTGAT CAGTACCTGT TCCGCCTCCG CTCCCGTCAC 
CTGAGCCAGA TCAAAGAAGC TGCCCTGGCC TTGAAGCTTG GCCACAGTGA GCTCTCCACT 
GCCCTGGAGC AGGCCGAGGA CTGGCTTCTG CATCTCCGTG CCCTGGCTGA GGAGCCCCAG 
AACAGCCTGC CAGATATCAT CATCTGGATG CTGCAGGGTG ACAAGCGTGT GGCCTACCAA 
CGGGTGCCTG CCCATGAAGT CCTCTTCTCC CGGCGGGGCC CCAGCTACTG CGGCAGGAAC 
TGTGGGAATC TGCAGACCAT CTTTCTGAAA TATCCTATGG AAGGCATGCC TGGGCCCCGG 
ATGCCGGTGC AGATCCGGAT CAAGCTCTGG TTTGGGCTCT CAGTGGATGA GAAGGAATTC 
AATCAGTTTG CAGAGGGGAA GCTCTCTGTC TTTGCTGAAA CGTATGAGAA CCAGACAAAG 
CTGGCGCTGG TCGGGAACTG GGGCACGACG GGCCTCACCT ACCCCAAGTT TTCTGACGTC 
ACAGGCAAGA TCAAACTACC GAAGGATAGC TTCCGCCCCT CAGCTGGCTG GGCCTGGGCA 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ATGCTGACGC CGGTCATCTG 
AGCTTTGTGG AGGAGGTGTT TGAGAACCAG ACTCGGCTAC CTGGAGGCCA GTGGATCTAT 
ATGAGTGACA ACTACACCGA CGTGAATGGG GAGAAGGTGC TTCCAAAGGA CGATATTGAA 
TGCCCGCTGG GTTGGAAGTG GGAAGATGAA GAATGGTCCA CAGACCTCAA TCGGGCTGTT 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATACCCCCAG ACCGAAAGCC CAAACACTGG 
GTCCCTGTTG AGAAGATGTA TTACACTCAC AGGCGTCGAC GCTGGGTCCG ACTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AAGCACAGGC AGGCAGAGGC TGAGGGCGAG 
GGCTGGGAAT ACGCCTCTCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAT 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCAC TGGAGAAGAC GGGGCCTGCA 
GCGGTGTTTG CCCTTGAGGG GGCCCTGGGC GGCATGGTGG ATGACAAGAG CGAAGATTCC 
ATGTCTGTGT CTACCCTGAG CTTTGGCGTT AACAGACCCA CAATCTCCTG CATCTTTGAC 
TATGGGAACC GCTACCATCT GCGTTGCTAC CTGTACCAGG CCCGGGACCT GCCCGCCATG 
GACAAAGACT CCTTCTCTGA TCCCTATGCC ATTGTCTCCT TCCTGCATCA AAGCCAGAAG 
ACGGTGGTGG AGAAGAACAC CCTGAACCCC ACCTGGGATC AAACCCTCAT CTTCTATGAG 
ATAGAGATCT TTGGCGAGCC GGCCAGTGTC GCCGAGCGGC CACCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA TGGCGCTGAC GAGTTCATGG GCCGCTGTAT TTGTCAGCCA 
AGCCTGGAAC GGATGCCCCG CCTGGCCTGG TTCCCACTGA CCAGGGGCAG CCAGCCTGCC 
GGAGAGCTGC TGGCAGCGTT CGAGCTTATC CAGAGAGAGA AGCCAGCCAT CCATCACATC 
CCTGGCTTTG AGATGCACGA AACTTCAAGG ATTTTGGATG AGACGGAGGA CACAGACTTG 
CCTTACCCAC CACCTCAAAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GCTCTCCAGC GTACAGCCAT CGAGATCCTG GCCTGGGGCC TTCGGAACAT AAAGAGCTAT 
CAGATGGCCA GCGTGTCTTC CCCCAGCCTC GTGGTGGAGT GTGGTGGGCA GACAGTGCAG 
TCCTGTGTCA TCAGAAACCT TCGGAAGAAC CCCAACTTTG ACGTCTGTAC TCTGTTCATG 
GAAGTGATGT TACCCAGAGA GGACCTCTAC TGTCCCCCTA TTGTGGTCAA GGTCATTGAT 
AATCGCCAGT TTGGCCGCAG GCCAGTTGTG GGTCAGTGCA CCATTCGCTC CCTGGAGAAC 
TTCCTGTGTG ACCCGTACTC AGCAGAGAGT CCATCCCCGC AAGGTGGCCC AGATGACGTG 
AGCTTACTCA GCCCTGGGGA AGACGTGCTC ATTGACATTG ATGACAAGGA GCCTCTCATC 
CCTGTCCAGG AGGAGGAGTT CATTGACTGG TGGAGCAAAT TCTTTGCTTC TATCGGGGAG 
AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGGAGGC CTGTCCGACT TTTGTAATAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGACACAGAA GATCCATCTG TCATCGGAGA ATTTAAGGGT 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAT CCAGCCATCC CTATGCCCCC GAGACAATTC 
CATCAGCTGG CTGCTCAGGG TCCCCAGGAA TGCTTGGTCC GGATTTACAT CATCCGAGCA 
TTTGGCTTAC AGCCCAAGGA CCCCAATGGC AAGTGCGACC CTTACATCAA GATCTCTATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCTT GTACCCTGGA GCCTGTATTT 
GGAAAGATGT TTGAACTCAC CTGCACTCTA CCTCTGGAGA AGGACTTGAA GATCACACTC 
TATGACTATG ACCTGCTCTC CAAGGATGAG AAGATTGGGG AGACAGTCAT TGATCTGGAG 
AATAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGCCTTC CACAGACCTA CTGTGTCTCT 
GGACCAAATC AGTGGAGAGA TCAACTCCGC CCTTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCACAGGG TCAAGGCCCC CGTGTACCGG ACAGACCGAG TGACGTTTCA GGATAAGGAA 
TACACCATTC AGGAGATAGA GGCTGGCAGA CTCCCAAACC CACACCTGGG CCCGGTGGAG 
GAACGCTTAG CCCTGCATGT CCTTCAACAA CAAGGCTTGG TCCCTGAGCA TGTGGAGTCT 
CGGCCTCTGT ACAGTCCTCT GCAGCCAGAT ATTGAGCAGG GGAAGCTACA GATGTGGATT 
GACATATTTC CAAAGGTGCT GGGCCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAAA 
GCTCGAAGGT TTTTTCTGCG TTGTATTATT TGGAACACAA AAGATGTGAT CTTGGATGAC 
CTCAGCCTCA CGGGGGAGAA GATGAGTGAC ATCTATGTGA AAGGCTGGAT GGTGGGATTC 
GAAGAACACA AACAGAAGAC AGATGTGCAC TACCGTTCCC TGGGCGGGGA GGGCAATTTC 
AACTGGAGGT TCGTGTTTCC CTTCGACTAT CTGCCCGCTG AGCAAGTCTG TGCTGTTGCC 
AAGAAGGATG CTTTCTGGAG ACTCGACAAG ACAGAGAGCA AGATCCCAGC ACGTGTGGTC 
TTCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGACT TTCTGGGCTC CCTGCAGCTG 
GATCTCAACC GAATGCCCAA GCCAGCCAAG ACGGCTGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACCT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA 
TGGTGGCCTT GTGTGACAGA GGAGGGCGCG AAGAAGATCC TGGCGGGGAA GCTGGAAATG 
ACCTTGGAGA TCGTTGCAGA AAGTGAACAT GAGGAGCGGC CTGCTGGCCA GGGTCGGGAT 
GAACCCAACA TGAATCCAAA GCTAGAAGAT CCCAGGCGCC CTGACACCTC TTTCCTGTGG 
TTCACCTCAC CATACAAGAC CATGAAGTTC ATCTTGTGGC GACGATTCCG GTGTGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTCCTG CTCTTCCTGG GTGTCTTTGT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTTAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236765.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236827.1 Rattus norvegicus dysferlin (Dysf), transcript variant X11, mRNA.

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CloneID	ORa27406
Clone ID Related Accession (Same CDS sequence)	XM_006236765.3
Accession Version	XM_006236765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ATTCTGGGAA GAAGTGGCCA 
GTCCCCACGG ACACAGGAGG AGAGGAAGAC ACGGAAGACC AGGGCCTGAC TGGAGATGAA 
GCTGAGCTCT TCCCAGATCC GAGTGTTGCT GTGGGCCCAG GGGGACCAAC GACGCCCAAG 
AAGCCACCTT CCCATCCTCC TCCCCACTAT CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT 
CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATT 
GAGGGGCGTC AGCTGCCTGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG 
CAGACCAAGC GGACTCGAAT CCAGAAGGGA AATAGCCCAC TTTTCAATGA GACTCTTTTC 
TTCAACGTAT TTGACTCTCC CTTGGAACTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCACGCT CCCTCAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC 
GTGTACAGAG AGCCCCGACA TGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGATCCCGAT 
GACTTCTCTG CAGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG 
GACGAAGCTC CCCTAGACAA GAAAGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC 
CTGCTTAGGC CCACTGGTGT GGCCCTTCGT GGTGCCCATT TTTGCCTGAA GCTCTTCCGG 
GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA AATCTTTGGC 
TTTGATAGCA ACAAGAAGAA CCTGGTGGAC CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA 
ATGCTTTGCA GCAAGATCCT GGAGAAGACT GCTAACCCTC AGTGGAACCA GAACATCACA 
TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACTC ACAATGACAC TGTGGCTACC ACCTACTTGG GTATGTCGAA AATCTCTGCC 
ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCAGC CACTGACCTG 
GATGACAACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ATGTGAACCT CTATGGCAGT 
CCCCGAGAGT TCACGGGCTT CCCAGATCCT TATGCGGAGC TCAACACAGG AAAGGGAGAA 
GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG TCCCTGGAGA CCAAACTGGT GGAGCACAGT 
GAACAGAAGG TGGAAGACCT TCCTGCTGAT GACATCTTGA GAGTGGAGAA GTACCTGCGG 
AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC TACTCAGCCA CAATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTCGAGGT CAGCATTGGG AACTACGGAA ACAAGTTTGA CACCACCTGC 
TTGCCGTTGG CCTCTACCAC CCAATACAGC CGGGCAGTCT TTGATGGATG CCACTACTAT 
TATTTGCCCT GGGGCAACGT GAAGCCTGTG GTAGTACTGT CCTCATACTG GGAAGACATC 
AGCCATCGAG TCGAGACTCA GAACCAGCTC CTCAGGATCG CTGACCGACT GGAAGCTAAC 
CTGGAGCAAG TCCACCTGGC TCTGAAGGCA CAGTGTTCCT CTGAGGATGT GGACGCCCTG 
GTGGCTCAGT TGACAGATGA GCTCCTTGCA GACTGCAGCC AGCCCCTGTG TGACATCCAT 
GAAATACCTT CTGCCACCCA CCTTGATCAG TACCTGTTCC GCCTCCGCTC CCGTCACCTG 
AGCCAGATCA AAGAAGCTGC CCTGGCCTTG AAGCTTGGCC ACAGTGAGCT CTCCACTGCC 
CTGGAGCAGG CCGAGGACTG GCTTCTGCAT CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC 
AGCCTGCCAG ATATCATCAT CTGGATGCTG CAGGGTGACA AGCGTGTGGC CTACCAACGG 
GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG CGGGGCCCCA GCTACTGCGG CAGGAACTGT 
GGGAATCTGC AGACCATCTT TCTGAAATAT CCTATGGAAG GCATGCCTGG GCCCCGGATG 
CCGGTGCAGA TCCGGATCAA GCTCTGGTTT GGGCTCTCAG TGGATGAGAA GGAATTCAAT 
CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT GCTGAAACGT ATGAGAACCA GACAAAGCTG 
GCGCTGGTCG GGAACTGGGG CACGACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACA 
GGCAAGATCA AACTACCGAA GGATAGCTTC CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA 
GACTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGATG CTGACGCCGG TCATCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACT CGGCTACCTG GAGGCCAGTG GATCTATATG 
AGTGACAACT ACACCGACGT GAATGGGGAG AAGGTGCTTC CAAAGGACGA TATTGAATGC 
CCGCTGGGTT GGAAGTGGGA AGATGAAGAA TGGTCCACAG ACCTCAATCG GGCTGTTGAT 
GAACAAGGCT GGGAGTACAG CATCACCATA CCCCCAGACC GAAAGCCCAA ACACTGGGTC 
CCTGTTGAGA AGATGTATTA CACTCACAGG CGTCGACGCT GGGTCCGACT GCGCAGGAGG 
GACCTCAGCC AGATGGAGGC ACTGAAGAAG CACAGGCAGG CAGAGGCTGA GGGCGAGGGC 
TGGGAATACG CCTCTCTCTT TGGCTGGAAG TTCCACCTGG AGTACCGCAA GACAGATGCC 
TTTCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCG 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC ATGGTGGATG ACAAGAGCGA AGATTCCATG 
TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC AGACCCACAA TCTCCTGCAT CTTTGACTAT 
GGGAACCGCT ACCATCTGCG TTGCTACCTG TACCAGGCCC GGGACCTGCC CGCCATGGAC 
AAAGACTCCT TCTCTGATCC CTATGCCATT GTCTCCTTCC TGCATCAAAG CCAGAAGACG 
GTGGTGGAGA AGAACACCCT GAACCCCACC TGGGATCAAA CCCTCATCTT CTATGAGATA 
GAGATCTTTG GCGAGCCGGC CAGTGTCGCC GAGCGGCCAC CCAGCATTGT GGTGGAGCTG 
TATGACCATG ACACCTATGG CGCTGACGAG TTCATGGGCC GCTGTATTTG TCAGCCAAGC 
CTGGAACGGA TGCCCCGCCT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCTGCCGGA 
GAGCTGCTGG CAGCGTTCGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA TCACATCCCT 
GGCTTTGAGA TGCACGAAAC TTCAAGGATT TTGGATGAGA CGGAGGACAC AGACTTGCCT 
TACCCACCAC CTCAAAGGGA GGCCAACATC TACATGGTTC CCCAGAACAT CAAGCCAGCT 
CTCCAGCGTA CAGCCATCGA GATCCTGGCC TGGGGCCTTC GGAACATAAA GAGCTATCAG 
ATGGCCAGCG TGTCTTCCCC CAGCCTCGTG GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC 
TGTGTCATCA GAAACCTTCG GAAGAACCCC AACTTTGACG TCTGTACTCT GTTCATGGAA 
GTGATGTTAC CCAGAGAGGA CCTCTACTGT CCCCCTATTG TGGTCAAGGT CATTGATAAT 
CGCCAGTTTG GCCGCAGGCC AGTTGTGGGT CAGTGCACCA TTCGCTCCCT GGAGAACTTC 
CTGTGTGACC CGTACTCAGC AGAGAGTCCA TCCCCGCAAG GTGGCCCAGA TGACGTGAGC 
TTACTCAGCC CTGGGGAAGA CGTGCTCATT GACATTGATG ACAAGGAGCC TCTCATCCCT 
GTCCAGGAGG AGGAGTTCAT TGACTGGTGG AGCAAATTCT TTGCTTCTAT CGGGGAGAGG 
GAAAAGTGTG GTTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGGAGGCCTG TCCGACTTTT GTAATACCTT CAAGCTCTAC 
CGGGGCAAGA CTCAGGAGGA CACAGAAGAT CCATCTGTCA TCGGAGAATT TAAGGGTCTC 
TTCAAAATTT ATCCCCTCCC AGAAGATCCA GCCATCCCTA TGCCCCCGAG ACAATTCCAT 
CAGCTGGCTG CTCAGGGTCC CCAGGAATGC TTGGTCCGGA TTTACATCAT CCGAGCATTT 
GGCTTACAGC CCAAGGACCC CAATGGCAAG TGCGACCCTT ACATCAAGAT CTCTATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCTTGTA CCCTGGAGCC TGTATTTGGA 
AAGATGTTTG AACTCACCTG CACTCTACCT CTGGAGAAGG ACTTGAAGAT CACACTCTAT 
GACTATGACC TGCTCTCCAA GGATGAGAAG ATTGGGGAGA CAGTCATTGA TCTGGAGAAT 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGCCTTCCAC AGACCTACTG TGTCTCTGGA 
CCAAATCAGT GGAGAGATCA ACTCCGCCCT TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CACAGGGTCA AGGCCCCCGT GTACCGGACA GACCGAGTGA CGTTTCAGGA TAAGGAATAC 
ACCATTCAGG AGATAGAGGC TGGCAGACTC CCAAACCCAC ACCTGGGCCC GGTGGAGGAA 
CGCTTAGCCC TGCATGTCCT TCAACAACAA GGCTTGGTCC CTGAGCATGT GGAGTCTCGG 
CCTCTGTACA GTCCTCTGCA GCCAGATATT GAGCAGGGGA AGCTACAGAT GTGGATTGAC 
ATATTTCCAA AGGTGCTGGG CCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAAAGCT 
CGAAGGTTTT TTCTGCGTTG TATTATTTGG AACACAAAAG ATGTGATCTT GGATGACCTC 
AGCCTCACGG GGGAGAAGAT GAGTGACATC TATGTGAAAG GCTGGATGGT GGGATTCGAA 
GAACACAAAC AGAAGACAGA TGTGCACTAC CGTTCCCTGG GCGGGGAGGG CAATTTCAAC 
TGGAGGTTCG TGTTTCCCTT CGACTATCTG CCCGCTGAGC AAGTCTGTGC TGTTGCCAAG 
AAGGATGCTT TCTGGAGACT CGACAAGACA GAGAGCAAGA TCCCAGCACG TGTGGTCTTC 
CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGACTTTC TGGGCTCCCT GCAGCTGGAT 
CTCAACCGAA TGCCCAAGCC AGCCAAGACG GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACCTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAGACAGT GAAAGGATGG 
TGGCCTTGTG TGACAGAGGA GGGCGCGAAG AAGATCCTGG CGGGGAAGCT GGAAATGACC 
TTGGAGATCG TTGCAGAAAG TGAACATGAG GAGCGGCCTG CTGGCCAGGG TCGGGATGAA 
CCCAACATGA ATCCAAAGCT AGAAGATCCC AGGCGCCCTG ACACCTCTTT CCTGTGGTTC 
ACCTCACCAT ACAAGACCAT GAAGTTCATC TTGTGGCGAC GATTCCGGTG TGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTCCTGCTC TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236827.1
CDS	398..6736
Translation	MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236765.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236765.3

ORF Insert Sequence:

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ATTCTGGGAA GAAGTGGCCA 
GTCCCCACGG ACACAGGAGG AGAGGAAGAC ACGGAAGACC AGGGCCTGAC TGGAGATGAA 
GCTGAGCTCT TCCCAGATCC GAGTGTTGCT GTGGGCCCAG GGGGACCAAC GACGCCCAAG 
AAGCCACCTT CCCATCCTCC TCCCCACTAT CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT 
CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATT 
GAGGGGCGTC AGCTGCCTGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG 
CAGACCAAGC GGACTCGAAT CCAGAAGGGA AATAGCCCAC TTTTCAATGA GACTCTTTTC 
TTCAACGTAT TTGACTCTCC CTTGGAACTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCACGCT CCCTCAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC 
GTGTACAGAG AGCCCCGACA TGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGATCCCGAT 
GACTTCTCTG CAGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG 
GACGAAGCTC CCCTAGACAA GAAAGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC 
CTGCTTAGGC CCACTGGTGT GGCCCTTCGT GGTGCCCATT TTTGCCTGAA GCTCTTCCGG 
GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA AATCTTTGGC 
TTTGATAGCA ACAAGAAGAA CCTGGTGGAC CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA 
ATGCTTTGCA GCAAGATCCT GGAGAAGACT GCTAACCCTC AGTGGAACCA GAACATCACA 
TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACTC ACAATGACAC TGTGGCTACC ACCTACTTGG GTATGTCGAA AATCTCTGCC 
ACTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTACTCA AGAGTCCAGC CACTGACCTG 
GATGACAACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ATGTGAACCT CTATGGCAGT 
CCCCGAGAGT TCACGGGCTT CCCAGATCCT TATGCGGAGC TCAACACAGG AAAGGGAGAA 
GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG TCCCTGGAGA CCAAACTGGT GGAGCACAGT 
GAACAGAAGG TGGAAGACCT TCCTGCTGAT GACATCTTGA GAGTGGAGAA GTACCTGCGG 
AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC TACTCAGCCA CAATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTCGAGGT CAGCATTGGG AACTACGGAA ACAAGTTTGA CACCACCTGC 
TTGCCGTTGG CCTCTACCAC CCAATACAGC CGGGCAGTCT TTGATGGATG CCACTACTAT 
TATTTGCCCT GGGGCAACGT GAAGCCTGTG GTAGTACTGT CCTCATACTG GGAAGACATC 
AGCCATCGAG TCGAGACTCA GAACCAGCTC CTCAGGATCG CTGACCGACT GGAAGCTAAC 
CTGGAGCAAG TCCACCTGGC TCTGAAGGCA CAGTGTTCCT CTGAGGATGT GGACGCCCTG 
GTGGCTCAGT TGACAGATGA GCTCCTTGCA GACTGCAGCC AGCCCCTGTG TGACATCCAT 
GAAATACCTT CTGCCACCCA CCTTGATCAG TACCTGTTCC GCCTCCGCTC CCGTCACCTG 
AGCCAGATCA AAGAAGCTGC CCTGGCCTTG AAGCTTGGCC ACAGTGAGCT CTCCACTGCC 
CTGGAGCAGG CCGAGGACTG GCTTCTGCAT CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC 
AGCCTGCCAG ATATCATCAT CTGGATGCTG CAGGGTGACA AGCGTGTGGC CTACCAACGG 
GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG CGGGGCCCCA GCTACTGCGG CAGGAACTGT 
GGGAATCTGC AGACCATCTT TCTGAAATAT CCTATGGAAG GCATGCCTGG GCCCCGGATG 
CCGGTGCAGA TCCGGATCAA GCTCTGGTTT GGGCTCTCAG TGGATGAGAA GGAATTCAAT 
CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT GCTGAAACGT ATGAGAACCA GACAAAGCTG 
GCGCTGGTCG GGAACTGGGG CACGACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACA 
GGCAAGATCA AACTACCGAA GGATAGCTTC CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA 
GACTGGTTCG TGTGTCCAGA GAAGACCCTG CTCCATGATG CTGACGCCGG TCATCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACT CGGCTACCTG GAGGCCAGTG GATCTATATG 
AGTGACAACT ACACCGACGT GAATGGGGAG AAGGTGCTTC CAAAGGACGA TATTGAATGC 
CCGCTGGGTT GGAAGTGGGA AGATGAAGAA TGGTCCACAG ACCTCAATCG GGCTGTTGAT 
GAACAAGGCT GGGAGTACAG CATCACCATA CCCCCAGACC GAAAGCCCAA ACACTGGGTC 
CCTGTTGAGA AGATGTATTA CACTCACAGG CGTCGACGCT GGGTCCGACT GCGCAGGAGG 
GACCTCAGCC AGATGGAGGC ACTGAAGAAG CACAGGCAGG CAGAGGCTGA GGGCGAGGGC 
TGGGAATACG CCTCTCTCTT TGGCTGGAAG TTCCACCTGG AGTACCGCAA GACAGATGCC 
TTTCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCG 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC ATGGTGGATG ACAAGAGCGA AGATTCCATG 
TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC AGACCCACAA TCTCCTGCAT CTTTGACTAT 
GGGAACCGCT ACCATCTGCG TTGCTACCTG TACCAGGCCC GGGACCTGCC CGCCATGGAC 
AAAGACTCCT TCTCTGATCC CTATGCCATT GTCTCCTTCC TGCATCAAAG CCAGAAGACG 
GTGGTGGAGA AGAACACCCT GAACCCCACC TGGGATCAAA CCCTCATCTT CTATGAGATA 
GAGATCTTTG GCGAGCCGGC CAGTGTCGCC GAGCGGCCAC CCAGCATTGT GGTGGAGCTG 
TATGACCATG ACACCTATGG CGCTGACGAG TTCATGGGCC GCTGTATTTG TCAGCCAAGC 
CTGGAACGGA TGCCCCGCCT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCTGCCGGA 
GAGCTGCTGG CAGCGTTCGA GCTTATCCAG AGAGAGAAGC CAGCCATCCA TCACATCCCT 
GGCTTTGAGA TGCACGAAAC TTCAAGGATT TTGGATGAGA CGGAGGACAC AGACTTGCCT 
TACCCACCAC CTCAAAGGGA GGCCAACATC TACATGGTTC CCCAGAACAT CAAGCCAGCT 
CTCCAGCGTA CAGCCATCGA GATCCTGGCC TGGGGCCTTC GGAACATAAA GAGCTATCAG 
ATGGCCAGCG TGTCTTCCCC CAGCCTCGTG GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC 
TGTGTCATCA GAAACCTTCG GAAGAACCCC AACTTTGACG TCTGTACTCT GTTCATGGAA 
GTGATGTTAC CCAGAGAGGA CCTCTACTGT CCCCCTATTG TGGTCAAGGT CATTGATAAT 
CGCCAGTTTG GCCGCAGGCC AGTTGTGGGT CAGTGCACCA TTCGCTCCCT GGAGAACTTC 
CTGTGTGACC CGTACTCAGC AGAGAGTCCA TCCCCGCAAG GTGGCCCAGA TGACGTGAGC 
TTACTCAGCC CTGGGGAAGA CGTGCTCATT GACATTGATG ACAAGGAGCC TCTCATCCCT 
GTCCAGGAGG AGGAGTTCAT TGACTGGTGG AGCAAATTCT TTGCTTCTAT CGGGGAGAGG 
GAAAAGTGTG GTTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGGAGGCCTG TCCGACTTTT GTAATACCTT CAAGCTCTAC 
CGGGGCAAGA CTCAGGAGGA CACAGAAGAT CCATCTGTCA TCGGAGAATT TAAGGGTCTC 
TTCAAAATTT ATCCCCTCCC AGAAGATCCA GCCATCCCTA TGCCCCCGAG ACAATTCCAT 
CAGCTGGCTG CTCAGGGTCC CCAGGAATGC TTGGTCCGGA TTTACATCAT CCGAGCATTT 
GGCTTACAGC CCAAGGACCC CAATGGCAAG TGCGACCCTT ACATCAAGAT CTCTATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCTTGTA CCCTGGAGCC TGTATTTGGA 
AAGATGTTTG AACTCACCTG CACTCTACCT CTGGAGAAGG ACTTGAAGAT CACACTCTAT 
GACTATGACC TGCTCTCCAA GGATGAGAAG ATTGGGGAGA CAGTCATTGA TCTGGAGAAT 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGCCTTCCAC AGACCTACTG TGTCTCTGGA 
CCAAATCAGT GGAGAGATCA ACTCCGCCCT TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CACAGGGTCA AGGCCCCCGT GTACCGGACA GACCGAGTGA CGTTTCAGGA TAAGGAATAC 
ACCATTCAGG AGATAGAGGC TGGCAGACTC CCAAACCCAC ACCTGGGCCC GGTGGAGGAA 
CGCTTAGCCC TGCATGTCCT TCAACAACAA GGCTTGGTCC CTGAGCATGT GGAGTCTCGG 
CCTCTGTACA GTCCTCTGCA GCCAGATATT GAGCAGGGGA AGCTACAGAT GTGGATTGAC 
ATATTTCCAA AGGTGCTGGG CCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAAAGCT 
CGAAGGTTTT TTCTGCGTTG TATTATTTGG AACACAAAAG ATGTGATCTT GGATGACCTC 
AGCCTCACGG GGGAGAAGAT GAGTGACATC TATGTGAAAG GCTGGATGGT GGGATTCGAA 
GAACACAAAC AGAAGACAGA TGTGCACTAC CGTTCCCTGG GCGGGGAGGG CAATTTCAAC 
TGGAGGTTCG TGTTTCCCTT CGACTATCTG CCCGCTGAGC AAGTCTGTGC TGTTGCCAAG 
AAGGATGCTT TCTGGAGACT CGACAAGACA GAGAGCAAGA TCCCAGCACG TGTGGTCTTC 
CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGACTTTC TGGGCTCCCT GCAGCTGGAT 
CTCAACCGAA TGCCCAAGCC AGCCAAGACG GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACCTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAGACAGT GAAAGGATGG 
TGGCCTTGTG TGACAGAGGA GGGCGCGAAG AAGATCCTGG CGGGGAAGCT GGAAATGACC 
TTGGAGATCG TTGCAGAAAG TGAACATGAG GAGCGGCCTG CTGGCCAGGG TCGGGATGAA 
CCCAACATGA ATCCAAAGCT AGAAGATCCC AGGCGCCCTG ACACCTCTTT CCTGTGGTTC 
ACCTCACCAT ACAAGACCAT GAAGTTCATC TTGTGGCGAC GATTCCGGTG TGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTCCTGCTC TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236766.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236828.1 Rattus norvegicus dysferlin (Dysf), transcript variant X12, mRNA.

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CloneID	ORa27407
Clone ID Related Accession (Same CDS sequence)	XM_006236766.3
Accession Version	XM_006236766.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTACTCA AGAGTCCAGC CACTGACCTG GATGACAACC TGGGCTTCCT CCCCACTTTT 
GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT 
TATGCGGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG 
TCCCTGGAGA CCAAACTGGT GGAGCACAGT GAACAGAAGG TGGAAGACCT TCCTGCTGAT 
GACATCTTGA GAGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC 
TACTCAGCCA CAATGCTGCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATTGGG 
AACTACGGAA ACAAGTTTGA CACCACCTGC TTGCCGTTGG CCTCTACCAC CCAATACAGC 
CGGGCAGTCT TTGATGGATG CCACTACTAT TATTTGCCCT GGGGCAACGT GAAGCCTGTG 
GTAGTACTGT CCTCATACTG GGAAGACATC AGCCATCGAG TCGAGACTCA GAACCAGCTC 
CTCAGGATCG CTGACCGACT GGAAGCTAAC CTGGAGCAAG TCCACCTGGC TCTGAAGGCA 
CAGTGTTCCT CTGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA 
GACTGCAGCC AGCCCCTGTG TGACATCCAT GAAATACCTT CTGCCACCCA CCTTGATCAG 
TACCTGTTCC GCCTCCGCTC CCGTCACCTG AGCCAGATCA AAGAAGCTGC CCTGGCCTTG 
AAGCTTGGCC ACAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT 
CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC AGCCTGCCAG ATATCATCAT CTGGATGCTG 
CAGGGTGACA AGCGTGTGGC CTACCAACGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG 
CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAATCTGC AGACCATCTT TCTGAAATAT 
CCTATGGAAG GCATGCCTGG GCCCCGGATG CCGGTGCAGA TCCGGATCAA GCTCTGGTTT 
GGGCTCTCAG TGGATGAGAA GGAATTCAAT CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT 
GCTGAAACGT ATGAGAACCA GACAAAGCTG GCGCTGGTCG GGAACTGGGG CACGACGGGC 
CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AACTACCGAA GGATAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGATG CTGACGCCGG TCATCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACT 
CGGCTACCTG GAGGCCAGTG GATCTATATG AGTGACAACT ACACCGACGT GAATGGGGAG 
AAGGTGCTTC CAAAGGACGA TATTGAATGC CCGCTGGGTT GGAAGTGGGA AGATGAAGAA 
TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA 
CCCCCAGACC GAAAGCCCAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG 
CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAAG 
CACAGGCAGG CAGAGGCTGA GGGCGAGGGC TGGGAATACG CCTCTCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGATGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCACTGG AGAAGACGGG GCCTGCAGCG GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC 
ATGGTGGATG ACAAGAGCGA AGATTCCATG TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC 
AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG TTGCTACCTG 
TACCAGGCCC GGGACCTGCC CGCCATGGAC AAAGACTCCT TCTCTGATCC CTATGCCATT 
GTCTCCTTCC TGCATCAAAG CCAGAAGACG GTGGTGGAGA AGAACACCCT GAACCCCACC 
TGGGATCAAA CCCTCATCTT CTATGAGATA GAGATCTTTG GCGAGCCGGC CAGTGTCGCC 
GAGCGGCCAC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTATGG CGCTGACGAG 
TTCATGGGCC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC 
CCACTGACCA GGGGCAGCCA GCCTGCCGGA GAGCTGCTGG CAGCGTTCGA GCTTATCCAG 
AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCACGAAAC TTCAAGGATT 
TTGGATGAGA CGGAGGACAC AGACTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGCT CTCCAGCGTA CAGCCATCGA GATCCTGGCC 
TGGGGCCTTC GGAACATAAA GAGCTATCAG ATGGCCAGCG TGTCTTCCCC CAGCCTCGTG 
GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC 
AACTTTGACG TCTGTACTCT GTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT 
CCCCCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCAGGCC AGTTGTGGGT 
CAGTGCACCA TTCGCTCCCT GGAGAACTTC CTGTGTGACC CGTACTCAGC AGAGAGTCCA 
TCCCCGCAAG GTGGCCCAGA TGACGTGAGC TTACTCAGCC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ACAAGGAGCC TCTCATCCCT GTCCAGCTTG CAGATGGGCT GTCAAGCTTG 
GGCCCCACTA ACCTGATGCC TTCTCCGTCC AGTCCTCATG AGGAGGAGTT CATTGACTGG 
TGGAGCAAAT TCTTTGCTTC TATCGGGGAG AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG 
GATTTTGACA CCCTGAAGGT CTATGACACA CAGCTGGAGA ATGTGGAGGC CTTTGGAGGC 
CTGTCCGACT TTTGTAATAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGACACAGAA 
GATCCATCTG TCATCGGAGA ATTTAAGGGT CTCTTCAAAA TTTATCCCCT CCCAGAAGAT 
CCAGCCATCC CTATGCCCCC GAGACAATTC CATCAGCTGG CTGCTCAGGG TCCCCAGGAA 
TGCTTGGTCC GGATTTACAT CATCCGAGCA TTTGGCTTAC AGCCCAAGGA CCCCAATGGC 
AAGTGCGACC CTTACATCAA GATCTCTATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATCCCTT GTACCCTGGA GCCTGTATTT GGAAAGATGT TTGAACTCAC CTGCACTCTA 
CCTCTGGAGA AGGACTTGAA GATCACACTC TATGACTATG ACCTGCTCTC CAAGGATGAG 
AAGATTGGGG AGACAGTCAT TGATCTGGAG AATAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGTGGCCTTC CACAGACCTA CTGTGTCTCT GGACCAAATC AGTGGAGAGA TCAACTCCGC 
CCTTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCACAGGG TCAAGGCCCC CGTGTACCGG 
ACAGACCGAG TGACGTTTCA GGATAAGGAA TACACCATTC AGGAGATAGA GGCTGGCAGA 
CTCCCAAACC CACACCTGGG CCCGGTGGAG GAACGCTTAG CCCTGCATGT CCTTCAACAA 
CAAGGCTTGG TCCCTGAGCA TGTGGAGTCT CGGCCTCTGT ACAGTCCTCT GCAGCCAGAT 
ATTGAGCAGG GGAAGCTACA GATGTGGATT GACATATTTC CAAAGGTGCT GGGCCGGCCT 
GGACCTCCCT TCAACATCAC CCCACGGAAA GCTCGAAGGT TTTTTCTGCG TTGTATTATT 
TGGAACACAA AAGATGTGAT CTTGGATGAC CTCAGCCTCA CGGGGGAGAA GATGAGTGAC 
ATCTATGTGA AAGGCTGGAT GGTGGGATTC GAAGAACACA AACAGAAGAC AGATGTGCAC 
TACCGTTCCC TGGGCGGGGA GGGCAATTTC AACTGGAGGT TCGTGTTTCC CTTCGACTAT 
CTGCCCGCTG AGCAAGTCTG TGCTGTTGCC AAGAAGGATG CTTTCTGGAG ACTCGACAAG 
ACAGAGAGCA AGATCCCAGC ACGTGTGGTC TTCCAGATTT GGGACAATGA CAAGTTCTCC 
TTTGATGACT TTCTGGGCTC CCTGCAGCTG GATCTCAACC GAATGCCCAA GCCAGCCAAG 
ACGGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACCT TCCACCCAGA ATGGTTTGTG 
TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA TGGTGGCCTT GTGTGACAGA GGAGGGCGCG 
AAGAAGATCC TGGCGGGGAA GCTGGAAATG ACCTTGGAGA TCGTTGCAGA AAGTGAACAT 
GAGGAGCGGC CTGCTGGCCA GGGTCGGGAT GAACCCAACA TGAATCCAAA GCTAGAAGAT 
CCCAGGCGCC CTGACACCTC TTTCCTGTGG TTCACCTCAC CATACAAGAC CATGAAGTTC 
ATCTTGTGGC GACGATTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTCCTG 
CTCTTCCTGG GTGTCTTTGT CTACGCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG 
CCCTTTAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236828.1
CDS	702..7013
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236766.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236766.3

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTACTCA AGAGTCCAGC CACTGACCTG GATGACAACC TGGGCTTCCT CCCCACTTTT 
GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT 
TATGCGGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG 
TCCCTGGAGA CCAAACTGGT GGAGCACAGT GAACAGAAGG TGGAAGACCT TCCTGCTGAT 
GACATCTTGA GAGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC 
TACTCAGCCA CAATGCTGCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATTGGG 
AACTACGGAA ACAAGTTTGA CACCACCTGC TTGCCGTTGG CCTCTACCAC CCAATACAGC 
CGGGCAGTCT TTGATGGATG CCACTACTAT TATTTGCCCT GGGGCAACGT GAAGCCTGTG 
GTAGTACTGT CCTCATACTG GGAAGACATC AGCCATCGAG TCGAGACTCA GAACCAGCTC 
CTCAGGATCG CTGACCGACT GGAAGCTAAC CTGGAGCAAG TCCACCTGGC TCTGAAGGCA 
CAGTGTTCCT CTGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA 
GACTGCAGCC AGCCCCTGTG TGACATCCAT GAAATACCTT CTGCCACCCA CCTTGATCAG 
TACCTGTTCC GCCTCCGCTC CCGTCACCTG AGCCAGATCA AAGAAGCTGC CCTGGCCTTG 
AAGCTTGGCC ACAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT 
CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC AGCCTGCCAG ATATCATCAT CTGGATGCTG 
CAGGGTGACA AGCGTGTGGC CTACCAACGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG 
CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAATCTGC AGACCATCTT TCTGAAATAT 
CCTATGGAAG GCATGCCTGG GCCCCGGATG CCGGTGCAGA TCCGGATCAA GCTCTGGTTT 
GGGCTCTCAG TGGATGAGAA GGAATTCAAT CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT 
GCTGAAACGT ATGAGAACCA GACAAAGCTG GCGCTGGTCG GGAACTGGGG CACGACGGGC 
CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AACTACCGAA GGATAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGATG CTGACGCCGG TCATCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACT 
CGGCTACCTG GAGGCCAGTG GATCTATATG AGTGACAACT ACACCGACGT GAATGGGGAG 
AAGGTGCTTC CAAAGGACGA TATTGAATGC CCGCTGGGTT GGAAGTGGGA AGATGAAGAA 
TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA 
CCCCCAGACC GAAAGCCCAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG 
CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAAG 
CACAGGCAGG CAGAGGCTGA GGGCGAGGGC TGGGAATACG CCTCTCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGATGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCACTGG AGAAGACGGG GCCTGCAGCG GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC 
ATGGTGGATG ACAAGAGCGA AGATTCCATG TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC 
AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG TTGCTACCTG 
TACCAGGCCC GGGACCTGCC CGCCATGGAC AAAGACTCCT TCTCTGATCC CTATGCCATT 
GTCTCCTTCC TGCATCAAAG CCAGAAGACG GTGGTGGAGA AGAACACCCT GAACCCCACC 
TGGGATCAAA CCCTCATCTT CTATGAGATA GAGATCTTTG GCGAGCCGGC CAGTGTCGCC 
GAGCGGCCAC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTATGG CGCTGACGAG 
TTCATGGGCC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC 
CCACTGACCA GGGGCAGCCA GCCTGCCGGA GAGCTGCTGG CAGCGTTCGA GCTTATCCAG 
AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCACGAAAC TTCAAGGATT 
TTGGATGAGA CGGAGGACAC AGACTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGCT CTCCAGCGTA CAGCCATCGA GATCCTGGCC 
TGGGGCCTTC GGAACATAAA GAGCTATCAG ATGGCCAGCG TGTCTTCCCC CAGCCTCGTG 
GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC 
AACTTTGACG TCTGTACTCT GTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT 
CCCCCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCAGGCC AGTTGTGGGT 
CAGTGCACCA TTCGCTCCCT GGAGAACTTC CTGTGTGACC CGTACTCAGC AGAGAGTCCA 
TCCCCGCAAG GTGGCCCAGA TGACGTGAGC TTACTCAGCC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ACAAGGAGCC TCTCATCCCT GTCCAGCTTG CAGATGGGCT GTCAAGCTTG 
GGCCCCACTA ACCTGATGCC TTCTCCGTCC AGTCCTCATG AGGAGGAGTT CATTGACTGG 
TGGAGCAAAT TCTTTGCTTC TATCGGGGAG AGGGAAAAGT GTGGTTCCTA CCTGGAGAAG 
GATTTTGACA CCCTGAAGGT CTATGACACA CAGCTGGAGA ATGTGGAGGC CTTTGGAGGC 
CTGTCCGACT TTTGTAATAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGACACAGAA 
GATCCATCTG TCATCGGAGA ATTTAAGGGT CTCTTCAAAA TTTATCCCCT CCCAGAAGAT 
CCAGCCATCC CTATGCCCCC GAGACAATTC CATCAGCTGG CTGCTCAGGG TCCCCAGGAA 
TGCTTGGTCC GGATTTACAT CATCCGAGCA TTTGGCTTAC AGCCCAAGGA CCCCAATGGC 
AAGTGCGACC CTTACATCAA GATCTCTATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATCCCTT GTACCCTGGA GCCTGTATTT GGAAAGATGT TTGAACTCAC CTGCACTCTA 
CCTCTGGAGA AGGACTTGAA GATCACACTC TATGACTATG ACCTGCTCTC CAAGGATGAG 
AAGATTGGGG AGACAGTCAT TGATCTGGAG AATAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGTGGCCTTC CACAGACCTA CTGTGTCTCT GGACCAAATC AGTGGAGAGA TCAACTCCGC 
CCTTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCACAGGG TCAAGGCCCC CGTGTACCGG 
ACAGACCGAG TGACGTTTCA GGATAAGGAA TACACCATTC AGGAGATAGA GGCTGGCAGA 
CTCCCAAACC CACACCTGGG CCCGGTGGAG GAACGCTTAG CCCTGCATGT CCTTCAACAA 
CAAGGCTTGG TCCCTGAGCA TGTGGAGTCT CGGCCTCTGT ACAGTCCTCT GCAGCCAGAT 
ATTGAGCAGG GGAAGCTACA GATGTGGATT GACATATTTC CAAAGGTGCT GGGCCGGCCT 
GGACCTCCCT TCAACATCAC CCCACGGAAA GCTCGAAGGT TTTTTCTGCG TTGTATTATT 
TGGAACACAA AAGATGTGAT CTTGGATGAC CTCAGCCTCA CGGGGGAGAA GATGAGTGAC 
ATCTATGTGA AAGGCTGGAT GGTGGGATTC GAAGAACACA AACAGAAGAC AGATGTGCAC 
TACCGTTCCC TGGGCGGGGA GGGCAATTTC AACTGGAGGT TCGTGTTTCC CTTCGACTAT 
CTGCCCGCTG AGCAAGTCTG TGCTGTTGCC AAGAAGGATG CTTTCTGGAG ACTCGACAAG 
ACAGAGAGCA AGATCCCAGC ACGTGTGGTC TTCCAGATTT GGGACAATGA CAAGTTCTCC 
TTTGATGACT TTCTGGGCTC CCTGCAGCTG GATCTCAACC GAATGCCCAA GCCAGCCAAG 
ACGGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACCT TCCACCCAGA ATGGTTTGTG 
TCCCTTTTTG AGCAGAAGAC AGTGAAAGGA TGGTGGCCTT GTGTGACAGA GGAGGGCGCG 
AAGAAGATCC TGGCGGGGAA GCTGGAAATG ACCTTGGAGA TCGTTGCAGA AAGTGAACAT 
GAGGAGCGGC CTGCTGGCCA GGGTCGGGAT GAACCCAACA TGAATCCAAA GCTAGAAGAT 
CCCAGGCGCC CTGACACCTC TTTCCTGTGG TTCACCTCAC CATACAAGAC CATGAAGTTC 
ATCTTGTGGC GACGATTCCG GTGTGCCATC ATCCTCTTCA TCATCCTCTT CATCCTCCTG 
CTCTTCCTGG GTGTCTTTGT CTACGCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG 
CCCTTTAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236767.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236829.1 Rattus norvegicus dysferlin (Dysf), transcript variant X13, mRNA.

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CloneID	ORa27408
Clone ID Related Accession (Same CDS sequence)	XM_006236767.3
Accession Version	XM_006236767.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236767.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTACTCAAGA GTCCAGCCAC TGACCTGGAT GACAACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTATG TGAACCTCTA TGGCAGTCCC CGAGAGTTCA CGGGCTTCCC AGATCCTTAT 
GCGGAGCTCA ACACAGGAAA GGGAGAAGGT GTGGCATATA GGGGCAGGCT TCTGTTGTCC 
CTGGAGACCA AACTGGTGGA GCACAGTGAA CAGAAGGTGG AAGACCTTCC TGCTGATGAC 
ATCTTGAGAG TGGAGAAGTA CCTGCGGAGG CGCAAATATT CCCTCTTTGC TGCCTTCTAC 
TCAGCCACAA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TACGGAAACA AGTTTGACAC CACCTGCTTG CCGTTGGCCT CTACCACCCA ATACAGCCGG 
GCAGTCTTTG ATGGATGCCA CTACTATTAT TTGCCCTGGG GCAACGTGAA GCCTGTGGTA 
GTACTGTCCT CATACTGGGA AGACATCAGC CATCGAGTCG AGACTCAGAA CCAGCTCCTC 
AGGATCGCTG ACCGACTGGA AGCTAACCTG GAGCAAGTCC ACCTGGCTCT GAAGGCACAG 
TGTTCCTCTG AGGATGTGGA CGCCCTGGTG GCTCAGTTGA CAGATGAGCT CCTTGCAGAC 
TGCAGCCAGC CCCTGTGTGA CATCCATGAA ATACCTTCTG CCACCCACCT TGATCAGTAC 
CTGTTCCGCC TCCGCTCCCG TCACCTGAGC CAGATCAAAG AAGCTGCCCT GGCCTTGAAG 
CTTGGCCACA GTGAGCTCTC CACTGCCCTG GAGCAGGCCG AGGACTGGCT TCTGCATCTC 
CGTGCCCTGG CTGAGGAGCC CCAGAACAGC CTGCCAGATA TCATCATCTG GATGCTGCAG 
GGTGACAAGC GTGTGGCCTA CCAACGGGTG CCTGCCCATG AAGTCCTCTT CTCCCGGCGG 
GGCCCCAGCT ACTGCGGCAG GAACTGTGGG AATCTGCAGA CCATCTTTCT GAAATATCCT 
ATGGAAGGCA TGCCTGGGCC CCGGATGCCG GTGCAGATCC GGATCAAGCT CTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA ATTCAATCAG TTTGCAGAGG GGAAGCTCTC TGTCTTTGCT 
GAAACGTATG AGAACCAGAC AAAGCTGGCG CTGGTCGGGA ACTGGGGCAC GACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACAGGC AAGATCAAAC TACCGAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGGCCTG GGCAGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGATGCTG ACGCCGGTCA TCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACTCGG 
CTACCTGGAG GCCAGTGGAT CTATATGAGT GACAACTACA CCGACGTGAA TGGGGAGAAG 
GTGCTTCCAA AGGACGATAT TGAATGCCCG CTGGGTTGGA AGTGGGAAGA TGAAGAATGG 
TCCACAGACC TCAATCGGGC TGTTGATGAA CAAGGCTGGG AGTACAGCAT CACCATACCC 
CCAGACCGAA AGCCCAAACA CTGGGTCCCT GTTGAGAAGA TGTATTACAC TCACAGGCGT 
CGACGCTGGG TCCGACTGCG CAGGAGGGAC CTCAGCCAGA TGGAGGCACT GAAGAAGCAC 
AGGCAGGCAG AGGCTGAGGG CGAGGGCTGG GAATACGCCT CTCTCTTTGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC AGATGCCTTT CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCGGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCATG 
GTGGATGACA AGAGCGAAGA TTCCATGTCT GTGTCTACCC TGAGCTTTGG CGTTAACAGA 
CCCACAATCT CCTGCATCTT TGACTATGGG AACCGCTACC ATCTGCGTTG CTACCTGTAC 
CAGGCCCGGG ACCTGCCCGC CATGGACAAA GACTCCTTCT CTGATCCCTA TGCCATTGTC 
TCCTTCCTGC ATCAAAGCCA GAAGACGGTG GTGGAGAAGA ACACCCTGAA CCCCACCTGG 
GATCAAACCC TCATCTTCTA TGAGATAGAG ATCTTTGGCG AGCCGGCCAG TGTCGCCGAG 
CGGCCACCCA GCATTGTGGT GGAGCTGTAT GACCATGACA CCTATGGCGC TGACGAGTTC 
ATGGGCCGCT GTATTTGTCA GCCAAGCCTG GAACGGATGC CCCGCCTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC TGCCGGAGAG CTGCTGGCAG CGTTCGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCATCA CATCCCTGGC TTTGAGATGC ACGAAACTTC AAGGATTTTG 
GATGAGACGG AGGACACAGA CTTGCCTTAC CCACCACCTC AAAGGGAGGC CAACATCTAC 
ATGGTTCCCC AGAACATCAA GCCAGCTCTC CAGCGTACAG CCATCGAGAT CCTGGCCTGG 
GGCCTTCGGA ACATAAAGAG CTATCAGATG GCCAGCGTGT CTTCCCCCAG CCTCGTGGTG 
GAGTGTGGTG GGCAGACAGT GCAGTCCTGT GTCATCAGAA ACCTTCGGAA GAACCCCAAC 
TTTGACGTCT GTACTCTGTT CATGGAAGTG ATGTTACCCA GAGAGGACCT CTACTGTCCC 
CCTATTGTGG TCAAGGTCAT TGATAATCGC CAGTTTGGCC GCAGGCCAGT TGTGGGTCAG 
TGCACCATTC GCTCCCTGGA GAACTTCCTG TGTGACCCGT ACTCAGCAGA GAGTCCATCC 
CCGCAAGGTG GCCCAGATGA CGTGAGCTTA CTCAGCCCTG GGGAAGACGT GCTCATTGAC 
ATTGATGACA AGGAGCCTCT CATCCCTGTC CAGCTTGCAG ATGGGCTGTC AAGCTTGGGC 
CCCACTAACC TGATGCCTTC TCCGTCCAGT CCTCATGAGG AGGAGTTCAT TGACTGGTGG 
AGCAAATTCT TTGCTTCTAT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGGAGGCCTG 
TCCGACTTTT GTAATACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA CACAGAAGAT 
CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA 
GCCATCCCTA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC 
TTGGTCCGGA TTTACATCAT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG 
TGCGACCCTT ACATCAAGAT CTCTATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCTTGTA CCCTGGAGCC TGTATTTGGA AAGATGTTTG AACTCACCTG CACTCTACCT 
CTGGAGAAGG ACTTGAAGAT CACACTCTAT GACTATGACC TGCTCTCCAA GGATGAGAAG 
ATTGGGGAGA CAGTCATTGA TCTGGAGAAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGCCTTCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA ACTCCGCCCT 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGGTCA AGGCCCCCGT GTACCGGACA 
GACCGAGTGA CGTTTCAGGA TAAGGAATAC ACCATTCAGG AGATAGAGGC TGGCAGACTC 
CCAAACCCAC ACCTGGGCCC GGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAACAACAA 
GGCTTGGTCC CTGAGCATGT GGAGTCTCGG CCTCTGTACA GTCCTCTGCA GCCAGATATT 
GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAAAGCT CGAAGGTTTT TTCTGCGTTG TATTATTTGG 
AACACAAAAG ATGTGATCTT GGATGACCTC AGCCTCACGG GGGAGAAGAT GAGTGACATC 
TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAAC AGAAGACAGA TGTGCACTAC 
CGTTCCCTGG GCGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG 
CCCGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCTT TCTGGAGACT CGACAAGACA 
GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATTTGGG ACAATGACAA GTTCTCCTTT 
GATGACTTTC TGGGCTCCCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGCGAAG 
AAGATCCTGG CGGGGAAGCT GGAAATGACC TTGGAGATCG TTGCAGAAAG TGAACATGAG 
GAGCGGCCTG CTGGCCAGGG TCGGGATGAA CCCAACATGA ATCCAAAGCT AGAAGATCCC 
AGGCGCCCTG ACACCTCTTT CCTGTGGTTC ACCTCACCAT ACAAGACCAT GAAGTTCATC 
TTGTGGCGAC GATTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC 
TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236829.1
CDS	398..6706
Translation	MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236767.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236767.3

ORF Insert Sequence:

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTACTCAAGA GTCCAGCCAC TGACCTGGAT GACAACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTATG TGAACCTCTA TGGCAGTCCC CGAGAGTTCA CGGGCTTCCC AGATCCTTAT 
GCGGAGCTCA ACACAGGAAA GGGAGAAGGT GTGGCATATA GGGGCAGGCT TCTGTTGTCC 
CTGGAGACCA AACTGGTGGA GCACAGTGAA CAGAAGGTGG AAGACCTTCC TGCTGATGAC 
ATCTTGAGAG TGGAGAAGTA CCTGCGGAGG CGCAAATATT CCCTCTTTGC TGCCTTCTAC 
TCAGCCACAA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TACGGAAACA AGTTTGACAC CACCTGCTTG CCGTTGGCCT CTACCACCCA ATACAGCCGG 
GCAGTCTTTG ATGGATGCCA CTACTATTAT TTGCCCTGGG GCAACGTGAA GCCTGTGGTA 
GTACTGTCCT CATACTGGGA AGACATCAGC CATCGAGTCG AGACTCAGAA CCAGCTCCTC 
AGGATCGCTG ACCGACTGGA AGCTAACCTG GAGCAAGTCC ACCTGGCTCT GAAGGCACAG 
TGTTCCTCTG AGGATGTGGA CGCCCTGGTG GCTCAGTTGA CAGATGAGCT CCTTGCAGAC 
TGCAGCCAGC CCCTGTGTGA CATCCATGAA ATACCTTCTG CCACCCACCT TGATCAGTAC 
CTGTTCCGCC TCCGCTCCCG TCACCTGAGC CAGATCAAAG AAGCTGCCCT GGCCTTGAAG 
CTTGGCCACA GTGAGCTCTC CACTGCCCTG GAGCAGGCCG AGGACTGGCT TCTGCATCTC 
CGTGCCCTGG CTGAGGAGCC CCAGAACAGC CTGCCAGATA TCATCATCTG GATGCTGCAG 
GGTGACAAGC GTGTGGCCTA CCAACGGGTG CCTGCCCATG AAGTCCTCTT CTCCCGGCGG 
GGCCCCAGCT ACTGCGGCAG GAACTGTGGG AATCTGCAGA CCATCTTTCT GAAATATCCT 
ATGGAAGGCA TGCCTGGGCC CCGGATGCCG GTGCAGATCC GGATCAAGCT CTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA ATTCAATCAG TTTGCAGAGG GGAAGCTCTC TGTCTTTGCT 
GAAACGTATG AGAACCAGAC AAAGCTGGCG CTGGTCGGGA ACTGGGGCAC GACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACAGGC AAGATCAAAC TACCGAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGGCCTG GGCAGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGATGCTG ACGCCGGTCA TCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACTCGG 
CTACCTGGAG GCCAGTGGAT CTATATGAGT GACAACTACA CCGACGTGAA TGGGGAGAAG 
GTGCTTCCAA AGGACGATAT TGAATGCCCG CTGGGTTGGA AGTGGGAAGA TGAAGAATGG 
TCCACAGACC TCAATCGGGC TGTTGATGAA CAAGGCTGGG AGTACAGCAT CACCATACCC 
CCAGACCGAA AGCCCAAACA CTGGGTCCCT GTTGAGAAGA TGTATTACAC TCACAGGCGT 
CGACGCTGGG TCCGACTGCG CAGGAGGGAC CTCAGCCAGA TGGAGGCACT GAAGAAGCAC 
AGGCAGGCAG AGGCTGAGGG CGAGGGCTGG GAATACGCCT CTCTCTTTGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC AGATGCCTTT CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCGGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCATG 
GTGGATGACA AGAGCGAAGA TTCCATGTCT GTGTCTACCC TGAGCTTTGG CGTTAACAGA 
CCCACAATCT CCTGCATCTT TGACTATGGG AACCGCTACC ATCTGCGTTG CTACCTGTAC 
CAGGCCCGGG ACCTGCCCGC CATGGACAAA GACTCCTTCT CTGATCCCTA TGCCATTGTC 
TCCTTCCTGC ATCAAAGCCA GAAGACGGTG GTGGAGAAGA ACACCCTGAA CCCCACCTGG 
GATCAAACCC TCATCTTCTA TGAGATAGAG ATCTTTGGCG AGCCGGCCAG TGTCGCCGAG 
CGGCCACCCA GCATTGTGGT GGAGCTGTAT GACCATGACA CCTATGGCGC TGACGAGTTC 
ATGGGCCGCT GTATTTGTCA GCCAAGCCTG GAACGGATGC CCCGCCTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC TGCCGGAGAG CTGCTGGCAG CGTTCGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCATCA CATCCCTGGC TTTGAGATGC ACGAAACTTC AAGGATTTTG 
GATGAGACGG AGGACACAGA CTTGCCTTAC CCACCACCTC AAAGGGAGGC CAACATCTAC 
ATGGTTCCCC AGAACATCAA GCCAGCTCTC CAGCGTACAG CCATCGAGAT CCTGGCCTGG 
GGCCTTCGGA ACATAAAGAG CTATCAGATG GCCAGCGTGT CTTCCCCCAG CCTCGTGGTG 
GAGTGTGGTG GGCAGACAGT GCAGTCCTGT GTCATCAGAA ACCTTCGGAA GAACCCCAAC 
TTTGACGTCT GTACTCTGTT CATGGAAGTG ATGTTACCCA GAGAGGACCT CTACTGTCCC 
CCTATTGTGG TCAAGGTCAT TGATAATCGC CAGTTTGGCC GCAGGCCAGT TGTGGGTCAG 
TGCACCATTC GCTCCCTGGA GAACTTCCTG TGTGACCCGT ACTCAGCAGA GAGTCCATCC 
CCGCAAGGTG GCCCAGATGA CGTGAGCTTA CTCAGCCCTG GGGAAGACGT GCTCATTGAC 
ATTGATGACA AGGAGCCTCT CATCCCTGTC CAGCTTGCAG ATGGGCTGTC AAGCTTGGGC 
CCCACTAACC TGATGCCTTC TCCGTCCAGT CCTCATGAGG AGGAGTTCAT TGACTGGTGG 
AGCAAATTCT TTGCTTCTAT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGGAGGCCTG 
TCCGACTTTT GTAATACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA CACAGAAGAT 
CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA 
GCCATCCCTA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC 
TTGGTCCGGA TTTACATCAT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG 
TGCGACCCTT ACATCAAGAT CTCTATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCTTGTA CCCTGGAGCC TGTATTTGGA AAGATGTTTG AACTCACCTG CACTCTACCT 
CTGGAGAAGG ACTTGAAGAT CACACTCTAT GACTATGACC TGCTCTCCAA GGATGAGAAG 
ATTGGGGAGA CAGTCATTGA TCTGGAGAAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGCCTTCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA ACTCCGCCCT 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGGTCA AGGCCCCCGT GTACCGGACA 
GACCGAGTGA CGTTTCAGGA TAAGGAATAC ACCATTCAGG AGATAGAGGC TGGCAGACTC 
CCAAACCCAC ACCTGGGCCC GGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAACAACAA 
GGCTTGGTCC CTGAGCATGT GGAGTCTCGG CCTCTGTACA GTCCTCTGCA GCCAGATATT 
GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAAAGCT CGAAGGTTTT TTCTGCGTTG TATTATTTGG 
AACACAAAAG ATGTGATCTT GGATGACCTC AGCCTCACGG GGGAGAAGAT GAGTGACATC 
TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAAC AGAAGACAGA TGTGCACTAC 
CGTTCCCTGG GCGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG 
CCCGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCTT TCTGGAGACT CGACAAGACA 
GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATTTGGG ACAATGACAA GTTCTCCTTT 
GATGACTTTC TGGGCTCCCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGCGAAG 
AAGATCCTGG CGGGGAAGCT GGAAATGACC TTGGAGATCG TTGCAGAAAG TGAACATGAG 
GAGCGGCCTG CTGGCCAGGG TCGGGATGAA CCCAACATGA ATCCAAAGCT AGAAGATCCC 
AGGCGCCCTG ACACCTCTTT CCTGTGGTTC ACCTCACCAT ACAAGACCAT GAAGTTCATC 
TTGTGGCGAC GATTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC 
TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236768.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236830.1 Rattus norvegicus dysferlin (Dysf), transcript variant X14, mRNA.

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CloneID	ORa27409
Clone ID Related Accession (Same CDS sequence)	XM_006236768.3
Accession Version	XM_006236768.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGTGGATGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCG 
GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCATATAGGG GCAGGCTTCT GTTGTCCCTG 
GAGACCAAAC TGGTGGAGCA CAGTGAACAG AAGGTGGAAG ACCTTCCTGC TGATGACATC 
TTGAGAGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA 
GCCACAATGC TGCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC 
GGAAACAAGT TTGACACCAC CTGCTTGCCG TTGGCCTCTA CCACCCAATA CAGCCGGGCA 
GTCTTTGATG GATGCCACTA CTATTATTTG CCCTGGGGCA ACGTGAAGCC TGTGGTAGTA 
CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAGTCGAGA CTCAGAACCA GCTCCTCAGG 
ATCGCTGACC GACTGGAAGC TAACCTGGAG CAAGTCCACC TGGCTCTGAA GGCACAGTGT 
TCCTCTGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC 
AGCCAGCCCC TGTGTGACAT CCATGAAATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG 
TTCCGCCTCC GCTCCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCCCTGGC CTTGAAGCTT 
GGCCACAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT 
GCCCTGGCTG AGGAGCCCCA GAACAGCCTG CCAGATATCA TCATCTGGAT GCTGCAGGGT 
GACAAGCGTG TGGCCTACCA ACGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC 
CCCAGCTACT GCGGCAGGAA CTGTGGGAAT CTGCAGACCA TCTTTCTGAA ATATCCTATG 
GAAGGCATGC CTGGGCCCCG GATGCCGGTG CAGATCCGGA TCAAGCTCTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAATT CAATCAGTTT GCAGAGGGGA AGCTCTCTGT CTTTGCTGAA 
ACGTATGAGA ACCAGACAAA GCTGGCGCTG GTCGGGAACT GGGGCACGAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAACTAC CGAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC AGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GATGCTGACG CCGGTCATCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACTCGGCTA 
CCTGGAGGCC AGTGGATCTA TATGAGTGAC AACTACACCG ACGTGAATGG GGAGAAGGTG 
CTTCCAAAGG ACGATATTGA ATGCCCGCTG GGTTGGAAGT GGGAAGATGA AGAATGGTCC 
ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA 
GACCGAAAGC CCAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA 
CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AGGCACTGAA GAAGCACAGG 
CAGGCAGAGG CTGAGGGCGA GGGCTGGGAA TACGCCTCTC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA TGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCGGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCATGGTG 
GATGACAAGA GCGAAGATTC CATGTCTGTG TCTACCCTGA GCTTTGGCGT TAACAGACCC 
ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGTTGCTA CCTGTACCAG 
GCCCGGGACC TGCCCGCCAT GGACAAAGAC TCCTTCTCTG ATCCCTATGC CATTGTCTCC 
TTCCTGCATC AAAGCCAGAA GACGGTGGTG GAGAAGAACA CCCTGAACCC CACCTGGGAT 
CAAACCCTCA TCTTCTATGA GATAGAGATC TTTGGCGAGC CGGCCAGTGT CGCCGAGCGG 
CCACCCAGCA TTGTGGTGGA GCTGTATGAC CATGACACCT ATGGCGCTGA CGAGTTCATG 
GGCCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCTGC CGGAGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCACG AAACTTCAAG GATTTTGGAT 
GAGACGGAGG ACACAGACTT GCCTTACCCA CCACCTCAAA GGGAGGCCAA CATCTACATG 
GTTCCCCAGA ACATCAAGCC AGCTCTCCAG CGTACAGCCA TCGAGATCCT GGCCTGGGGC 
CTTCGGAACA TAAAGAGCTA TCAGATGGCC AGCGTGTCTT CCCCCAGCCT CGTGGTGGAG 
TGTGGTGGGC AGACAGTGCA GTCCTGTGTC ATCAGAAACC TTCGGAAGAA CCCCAACTTT 
GACGTCTGTA CTCTGTTCAT GGAAGTGATG TTACCCAGAG AGGACCTCTA CTGTCCCCCT 
ATTGTGGTCA AGGTCATTGA TAATCGCCAG TTTGGCCGCA GGCCAGTTGT GGGTCAGTGC 
ACCATTCGCT CCCTGGAGAA CTTCCTGTGT GACCCGTACT CAGCAGAGAG TCCATCCCCG 
CAAGGTGGCC CAGATGACGT GAGCTTACTC AGCCCTGGGG AAGACGTGCT CATTGACATT 
GATGACAAGG AGCCTCTCAT CCCTGTCCAG GAGGAGGAGT TCATTGACTG GTGGAGCAAA 
TTCTTTGCTT CTATCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGGAGG CCTGTCCGAC 
TTTTGTAATA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGACACAGA AGATCCATCT 
GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC 
CCTATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC 
CGGATTTACA TCATCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGCGAC 
CCTTACATCA AGATCTCTAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT 
TGTACCCTGG AGCCTGTATT TGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG 
AAGGACTTGA AGATCACACT CTATGACTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG 
GAGACAGTCA TTGATCTGGA GAATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGCCTT 
CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAACTCCG CCCTTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCACAGG GTCAAGGCCC CCGTGTACCG GACAGACCGA 
GTGACGTTTC AGGATAAGGA ATACACCATT CAGGAGATAG AGGCTGGCAG ACTCCCAAAC 
CCACACCTGG GCCCGGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAACA ACAAGGCTTG 
GTCCCTGAGC ATGTGGAGTC TCGGCCTCTG TACAGTCCTC TGCAGCCAGA TATTGAGCAG 
GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAA AGCTCGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA 
AAAGATGTGA TCTTGGATGA CCTCAGCCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG 
AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAACAGAAGA CAGATGTGCA CTACCGTTCC 
CTGGGCGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCCGCT 
GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCTTTCTGGA GACTCGACAA GACAGAGAGC 
AAGATCCCAG CACGTGTGGT CTTCCAGATT TGGGACAATG ACAAGTTCTC CTTTGATGAC 
TTTCTGGGCT CCCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGC GAAGAAGATC 
CTGGCGGGGA AGCTGGAAAT GACCTTGGAG ATCGTTGCAG AAAGTGAACA TGAGGAGCGG 
CCTGCTGGCC AGGGTCGGGA TGAACCCAAC ATGAATCCAA AGCTAGAAGA TCCCAGGCGC 
CCTGACACCT CTTTCCTGTG GTTCACCTCA CCATACAAGA CCATGAAGTT CATCTTGTGG 
CGACGATTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG 
GGTGTCTTTG TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTTAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236830.1
CDS	702..7004
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236768.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236768.3

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATATTCTGG GAAGAAGTGG 
CCAGTCCCCA CGGACACAGG AGGAGAGGAA GACACGGAAG ACCAGGGCCT GACTGGAGAT 
GAAGCTGAGC TCTTCCCAGA TCCGAGTGTT GCTGTGGGCC CAGGGGGACC AACGACGCCC 
AAGAAGCCAC CTTCCCATCC TCCTCCCCAC TATCCAGGGG GGAAAAGGAA AAGAAGCTCT 
GCTCCACCTA GAAAGCTGCT GTCAGACAAG CCACAGGACT TCCAGATCAG AGTCCAGGTG 
ATTGAGGGGC GTCAGCTGCC TGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACAGCTGCT 
GGGCAGACCA AGCGGACTCG AATCCAGAAG GGAAATAGCC CACTTTTCAA TGAGACTCTT 
TTCTTCAACG TATTTGACTC TCCCTTGGAA CTGTTTGATG AGCCCATCTT CATCACGGTG 
GTAGACTCAC GCTCCCTCAG GACAGATGCC CTCCTTGGCG AGTTCCGGAT GGATGTGGGC 
ACCGTGTACA GAGAGCCCCG ACATGCTTAT CTCCGGAAGT GGCTGCTGCT CTCCGATCCC 
GATGACTTCT CTGCAGGGGC CAGAGGCTAC CTCAAAGCCA GCCTGTGTGT GCTGGGGCCT 
GGGGACGAAG CTCCCCTAGA CAAGAAAGAC CCTTCTGAAG ACAAAGAGGA CATTGAAGGC 
AACCTGCTTA GGCCCACTGG TGTGGCCCTT CGTGGTGCCC ATTTTTGCCT GAAGCTCTTC 
CGGGCTGAGG ACTTACCACA GATGGACGAT GCTGTGATGG ACAACGTCAA GCAAATCTTT 
GGCTTTGATA GCAACAAGAA GAACCTGGTG GACCCCTTTG TGGAAGTCAG CTTTGCTGGG 
AAAATGCTTT GCAGCAAGAT CCTGGAGAAG ACTGCTAACC CTCAGTGGAA CCAGAACATC 
ACATTGCCTG CGATGTTTCC CTCTATGTGT GAAAAAATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CTCACAATGA CACTGTGGCT ACCACCTACT TGGGTATGTC GAAAATCTCT 
GCCACTGGAG GAGAAATAGA AGTGGATGAC AACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTATGTGA ACCTCTATGG CAGTCCCCGA GAGTTCACGG GCTTCCCAGA TCCTTATGCG 
GAGCTCAACA CAGGAAAGGG AGAAGGTGTG GCATATAGGG GCAGGCTTCT GTTGTCCCTG 
GAGACCAAAC TGGTGGAGCA CAGTGAACAG AAGGTGGAAG ACCTTCCTGC TGATGACATC 
TTGAGAGTGG AGAAGTACCT GCGGAGGCGC AAATATTCCC TCTTTGCTGC CTTCTACTCA 
GCCACAATGC TGCAGGATGT GGATGATGCC ATCCAGTTCG AGGTCAGCAT TGGGAACTAC 
GGAAACAAGT TTGACACCAC CTGCTTGCCG TTGGCCTCTA CCACCCAATA CAGCCGGGCA 
GTCTTTGATG GATGCCACTA CTATTATTTG CCCTGGGGCA ACGTGAAGCC TGTGGTAGTA 
CTGTCCTCAT ACTGGGAAGA CATCAGCCAT CGAGTCGAGA CTCAGAACCA GCTCCTCAGG 
ATCGCTGACC GACTGGAAGC TAACCTGGAG CAAGTCCACC TGGCTCTGAA GGCACAGTGT 
TCCTCTGAGG ATGTGGACGC CCTGGTGGCT CAGTTGACAG ATGAGCTCCT TGCAGACTGC 
AGCCAGCCCC TGTGTGACAT CCATGAAATA CCTTCTGCCA CCCACCTTGA TCAGTACCTG 
TTCCGCCTCC GCTCCCGTCA CCTGAGCCAG ATCAAAGAAG CTGCCCTGGC CTTGAAGCTT 
GGCCACAGTG AGCTCTCCAC TGCCCTGGAG CAGGCCGAGG ACTGGCTTCT GCATCTCCGT 
GCCCTGGCTG AGGAGCCCCA GAACAGCCTG CCAGATATCA TCATCTGGAT GCTGCAGGGT 
GACAAGCGTG TGGCCTACCA ACGGGTGCCT GCCCATGAAG TCCTCTTCTC CCGGCGGGGC 
CCCAGCTACT GCGGCAGGAA CTGTGGGAAT CTGCAGACCA TCTTTCTGAA ATATCCTATG 
GAAGGCATGC CTGGGCCCCG GATGCCGGTG CAGATCCGGA TCAAGCTCTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAATT CAATCAGTTT GCAGAGGGGA AGCTCTCTGT CTTTGCTGAA 
ACGTATGAGA ACCAGACAAA GCTGGCGCTG GTCGGGAACT GGGGCACGAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACAGGCAAG ATCAAACTAC CGAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGGCCTGGGC AGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTGCTCCAT 
GATGCTGACG CCGGTCATCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACTCGGCTA 
CCTGGAGGCC AGTGGATCTA TATGAGTGAC AACTACACCG ACGTGAATGG GGAGAAGGTG 
CTTCCAAAGG ACGATATTGA ATGCCCGCTG GGTTGGAAGT GGGAAGATGA AGAATGGTCC 
ACAGACCTCA ATCGGGCTGT TGATGAACAA GGCTGGGAGT ACAGCATCAC CATACCCCCA 
GACCGAAAGC CCAAACACTG GGTCCCTGTT GAGAAGATGT ATTACACTCA CAGGCGTCGA 
CGCTGGGTCC GACTGCGCAG GAGGGACCTC AGCCAGATGG AGGCACTGAA GAAGCACAGG 
CAGGCAGAGG CTGAGGGCGA GGGCTGGGAA TACGCCTCTC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA TGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCGGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCATGGTG 
GATGACAAGA GCGAAGATTC CATGTCTGTG TCTACCCTGA GCTTTGGCGT TAACAGACCC 
ACAATCTCCT GCATCTTTGA CTATGGGAAC CGCTACCATC TGCGTTGCTA CCTGTACCAG 
GCCCGGGACC TGCCCGCCAT GGACAAAGAC TCCTTCTCTG ATCCCTATGC CATTGTCTCC 
TTCCTGCATC AAAGCCAGAA GACGGTGGTG GAGAAGAACA CCCTGAACCC CACCTGGGAT 
CAAACCCTCA TCTTCTATGA GATAGAGATC TTTGGCGAGC CGGCCAGTGT CGCCGAGCGG 
CCACCCAGCA TTGTGGTGGA GCTGTATGAC CATGACACCT ATGGCGCTGA CGAGTTCATG 
GGCCGCTGTA TTTGTCAGCC AAGCCTGGAA CGGATGCCCC GCCTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCTGC CGGAGAGCTG CTGGCAGCGT TCGAGCTTAT CCAGAGAGAG 
AAGCCAGCCA TCCATCACAT CCCTGGCTTT GAGATGCACG AAACTTCAAG GATTTTGGAT 
GAGACGGAGG ACACAGACTT GCCTTACCCA CCACCTCAAA GGGAGGCCAA CATCTACATG 
GTTCCCCAGA ACATCAAGCC AGCTCTCCAG CGTACAGCCA TCGAGATCCT GGCCTGGGGC 
CTTCGGAACA TAAAGAGCTA TCAGATGGCC AGCGTGTCTT CCCCCAGCCT CGTGGTGGAG 
TGTGGTGGGC AGACAGTGCA GTCCTGTGTC ATCAGAAACC TTCGGAAGAA CCCCAACTTT 
GACGTCTGTA CTCTGTTCAT GGAAGTGATG TTACCCAGAG AGGACCTCTA CTGTCCCCCT 
ATTGTGGTCA AGGTCATTGA TAATCGCCAG TTTGGCCGCA GGCCAGTTGT GGGTCAGTGC 
ACCATTCGCT CCCTGGAGAA CTTCCTGTGT GACCCGTACT CAGCAGAGAG TCCATCCCCG 
CAAGGTGGCC CAGATGACGT GAGCTTACTC AGCCCTGGGG AAGACGTGCT CATTGACATT 
GATGACAAGG AGCCTCTCAT CCCTGTCCAG GAGGAGGAGT TCATTGACTG GTGGAGCAAA 
TTCTTTGCTT CTATCGGGGA GAGGGAAAAG TGTGGTTCCT ACCTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGGAGG CCTGTCCGAC 
TTTTGTAATA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGACACAGA AGATCCATCT 
GTCATCGGAG AATTTAAGGG TCTCTTCAAA ATTTATCCCC TCCCAGAAGA TCCAGCCATC 
CCTATGCCCC CGAGACAATT CCATCAGCTG GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC 
CGGATTTACA TCATCCGAGC ATTTGGCTTA CAGCCCAAGG ACCCCAATGG CAAGTGCGAC 
CCTTACATCA AGATCTCTAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCT 
TGTACCCTGG AGCCTGTATT TGGAAAGATG TTTGAACTCA CCTGCACTCT ACCTCTGGAG 
AAGGACTTGA AGATCACACT CTATGACTAT GACCTGCTCT CCAAGGATGA GAAGATTGGG 
GAGACAGTCA TTGATCTGGA GAATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGCCTT 
CCACAGACCT ACTGTGTCTC TGGACCAAAT CAGTGGAGAG ATCAACTCCG CCCTTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCACAGG GTCAAGGCCC CCGTGTACCG GACAGACCGA 
GTGACGTTTC AGGATAAGGA ATACACCATT CAGGAGATAG AGGCTGGCAG ACTCCCAAAC 
CCACACCTGG GCCCGGTGGA GGAACGCTTA GCCCTGCATG TCCTTCAACA ACAAGGCTTG 
GTCCCTGAGC ATGTGGAGTC TCGGCCTCTG TACAGTCCTC TGCAGCCAGA TATTGAGCAG 
GGGAAGCTAC AGATGTGGAT TGACATATTT CCAAAGGTGC TGGGCCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAA AGCTCGAAGG TTTTTTCTGC GTTGTATTAT TTGGAACACA 
AAAGATGTGA TCTTGGATGA CCTCAGCCTC ACGGGGGAGA AGATGAGTGA CATCTATGTG 
AAAGGCTGGA TGGTGGGATT CGAAGAACAC AAACAGAAGA CAGATGTGCA CTACCGTTCC 
CTGGGCGGGG AGGGCAATTT CAACTGGAGG TTCGTGTTTC CCTTCGACTA TCTGCCCGCT 
GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT GCTTTCTGGA GACTCGACAA GACAGAGAGC 
AAGATCCCAG CACGTGTGGT CTTCCAGATT TGGGACAATG ACAAGTTCTC CTTTGATGAC 
TTTCTGGGCT CCCTGCAGCT GGATCTCAAC CGAATGCCCA AGCCAGCCAA GACGGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACC TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT TGTGTGACAG AGGAGGGCGC GAAGAAGATC 
CTGGCGGGGA AGCTGGAAAT GACCTTGGAG ATCGTTGCAG AAAGTGAACA TGAGGAGCGG 
CCTGCTGGCC AGGGTCGGGA TGAACCCAAC ATGAATCCAA AGCTAGAAGA TCCCAGGCGC 
CCTGACACCT CTTTCCTGTG GTTCACCTCA CCATACAAGA CCATGAAGTT CATCTTGTGG 
CGACGATTCC GGTGTGCCAT CATCCTCTTC ATCATCCTCT TCATCCTCCT GCTCTTCCTG 
GGTGTCTTTG TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTTAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236769.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236831.1 Rattus norvegicus dysferlin (Dysf), transcript variant X15, mRNA.

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CloneID	ORa27410
Clone ID Related Accession (Same CDS sequence)	XM_006236769.3
Accession Version	XM_006236769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236769.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ATTCTGGGAA GAAGTGGCCA 
GTCCCCACGG ACACAGGAGG AGAGGAAGAC ACGGAAGACC AGGGCCTGAC TGGAGATGAA 
GCTGAGCTCT TCCCAGATCC GAGTGTTGCT GTGGGCCCAG GGGGACCAAC GACGCCCAAG 
AAGCCACCTT CCCATCCTCC TCCCCACTAT CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT 
CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATT 
GAGGGGCGTC AGCTGCCTGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG 
CAGACCAAGC GGACTCGAAT CCAGAAGGGA AATAGCCCAC TTTTCAATGA GACTCTTTTC 
TTCAACGTAT TTGACTCTCC CTTGGAACTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCACGCT CCCTCAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC 
GTGTACAGAG AGCCCCGACA TGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGATCCCGAT 
GACTTCTCTG CAGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG 
GACGAAGCTC CCCTAGACAA GAAAGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC 
CTGCTTAGGC CCACTGGTGT GGCCCTTCGT GGTGCCCATT TTTGCCTGAA GCTCTTCCGG 
GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA AATCTTTGGC 
TTTGATAGCA ACAAGAAGAA CCTGGTGGAC CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA 
ATGCTTTGCA GCAAGATCCT GGAGAAGACT GCTAACCCTC AGTGGAACCA GAACATCACA 
TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACTC ACAATGACAC TGTGGCTACC ACCTACTTGG GTATGTCGAA AATCTCTGCC 
ACTGGAGGAG AAATAGAAGT GGATGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC 
TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCGGAG 
CTCAACACAG GAAAGGGAGA AGGTGTGGCA TATAGGGGCA GGCTTCTGTT GTCCCTGGAG 
ACCAAACTGG TGGAGCACAG TGAACAGAAG GTGGAAGACC TTCCTGCTGA TGACATCTTG 
AGAGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC 
ACAATGCTGC AGGATGTGGA TGATGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGA 
AACAAGTTTG ACACCACCTG CTTGCCGTTG GCCTCTACCA CCCAATACAG CCGGGCAGTC 
TTTGATGGAT GCCACTACTA TTATTTGCCC TGGGGCAACG TGAAGCCTGT GGTAGTACTG 
TCCTCATACT GGGAAGACAT CAGCCATCGA GTCGAGACTC AGAACCAGCT CCTCAGGATC 
GCTGACCGAC TGGAAGCTAA CCTGGAGCAA GTCCACCTGG CTCTGAAGGC ACAGTGTTCC 
TCTGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC 
CAGCCCCTGT GTGACATCCA TGAAATACCT TCTGCCACCC ACCTTGATCA GTACCTGTTC 
CGCCTCCGCT CCCGTCACCT GAGCCAGATC AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC 
CACAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC 
CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA GATATCATCA TCTGGATGCT GCAGGGTGAC 
AAGCGTGTGG CCTACCAACG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC 
AGCTACTGCG GCAGGAACTG TGGGAATCTG CAGACCATCT TTCTGAAATA TCCTATGGAA 
GGCATGCCTG GGCCCCGGAT GCCGGTGCAG ATCCGGATCA AGCTCTGGTT TGGGCTCTCA 
GTGGATGAGA AGGAATTCAA TCAGTTTGCA GAGGGGAAGC TCTCTGTCTT TGCTGAAACG 
TATGAGAACC AGACAAAGCT GGCGCTGGTC GGGAACTGGG GCACGACGGG CCTCACCTAC 
CCCAAGTTTT CTGACGTCAC AGGCAAGATC AAACTACCGA AGGATAGCTT CCGCCCCTCA 
GCTGGCTGGG CCTGGGCAGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAT 
GCTGACGCCG GTCATCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC TCGGCTACCT 
GGAGGCCAGT GGATCTATAT GAGTGACAAC TACACCGACG TGAATGGGGA GAAGGTGCTT 
CCAAAGGACG ATATTGAATG CCCGCTGGGT TGGAAGTGGG AAGATGAAGA ATGGTCCACA 
GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC 
CGAAAGCCCA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC 
TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAA GCACAGGCAG 
GCAGAGGCTG AGGGCGAGGG CTGGGAATAC GCCTCTCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACAGATGC CTTTCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCACTG 
GAGAAGACGG GGCCTGCAGC GGTGTTTGCC CTTGAGGGGG CCCTGGGCGG CATGGTGGAT 
GACAAGAGCG AAGATTCCAT GTCTGTGTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA 
ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GTTGCTACCT GTACCAGGCC 
CGGGACCTGC CCGCCATGGA CAAAGACTCC TTCTCTGATC CCTATGCCAT TGTCTCCTTC 
CTGCATCAAA GCCAGAAGAC GGTGGTGGAG AAGAACACCC TGAACCCCAC CTGGGATCAA 
ACCCTCATCT TCTATGAGAT AGAGATCTTT GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTATG GCGCTGACGA GTTCATGGGC 
CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC 
AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG 
CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCACGAAA CTTCAAGGAT TTTGGATGAG 
ACGGAGGACA CAGACTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCAGC TCTCCAGCGT ACAGCCATCG AGATCCTGGC CTGGGGCCTT 
CGGAACATAA AGAGCTATCA GATGGCCAGC GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT 
GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAC 
GTCTGTACTC TGTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCCCCTATT 
GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCAGGC CAGTTGTGGG TCAGTGCACC 
ATTCGCTCCC TGGAGAACTT CCTGTGTGAC CCGTACTCAG CAGAGAGTCC ATCCCCGCAA 
GGTGGCCCAG ATGACGTGAG CTTACTCAGC CCTGGGGAAG ACGTGCTCAT TGACATTGAT 
GACAAGGAGC CTCTCATCCC TGTCCAGGAG GAGGAGTTCA TTGACTGGTG GAGCAAATTC 
TTTGCTTCTA TCGGGGAGAG GGAAAAGTGT GGTTCCTACC TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGGAGGCCT GTCCGACTTT 
TGTAATACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG ACACAGAAGA TCCATCTGTC 
ATCGGAGAAT TTAAGGGTCT CTTCAAAATT TATCCCCTCC CAGAAGATCC AGCCATCCCT 
ATGCCCCCGA GACAATTCCA TCAGCTGGCT GCTCAGGGTC CCCAGGAATG CTTGGTCCGG 
ATTTACATCA TCCGAGCATT TGGCTTACAG CCCAAGGACC CCAATGGCAA GTGCGACCCT 
TACATCAAGA TCTCTATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCTTGT 
ACCCTGGAGC CTGTATTTGG AAAGATGTTT GAACTCACCT GCACTCTACC TCTGGAGAAG 
GACTTGAAGA TCACACTCTA TGACTATGAC CTGCTCTCCA AGGATGAGAA GATTGGGGAG 
ACAGTCATTG ATCTGGAGAA TAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGCCTTCCA 
CAGACCTACT GTGTCTCTGG ACCAAATCAG TGGAGAGATC AACTCCGCCC TTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCACAGGGTC AAGGCCCCCG TGTACCGGAC AGACCGAGTG 
ACGTTTCAGG ATAAGGAATA CACCATTCAG GAGATAGAGG CTGGCAGACT CCCAAACCCA 
CACCTGGGCC CGGTGGAGGA ACGCTTAGCC CTGCATGTCC TTCAACAACA AGGCTTGGTC 
CCTGAGCATG TGGAGTCTCG GCCTCTGTAC AGTCCTCTGC AGCCAGATAT TGAGCAGGGG 
AAGCTACAGA TGTGGATTGA CATATTTCCA AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAAAGC TCGAAGGTTT TTTCTGCGTT GTATTATTTG GAACACAAAA 
GATGTGATCT TGGATGACCT CAGCCTCACG GGGGAGAAGA TGAGTGACAT CTATGTGAAA 
GGCTGGATGG TGGGATTCGA AGAACACAAA CAGAAGACAG ATGTGCACTA CCGTTCCCTG 
GGCGGGGAGG GCAATTTCAA CTGGAGGTTC GTGTTTCCCT TCGACTATCT GCCCGCTGAG 
CAAGTCTGTG CTGTTGCCAA GAAGGATGCT TTCTGGAGAC TCGACAAGAC AGAGAGCAAG 
ATCCCAGCAC GTGTGGTCTT CCAGATTTGG GACAATGACA AGTTCTCCTT TGATGACTTT 
CTGGGCTCCC TGCAGCTGGA TCTCAACCGA ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACCTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAGACAG TGAAAGGATG GTGGCCTTGT GTGACAGAGG AGGGCGCGAA GAAGATCCTG 
GCGGGGAAGC TGGAAATGAC CTTGGAGATC GTTGCAGAAA GTGAACATGA GGAGCGGCCT 
GCTGGCCAGG GTCGGGATGA ACCCAACATG AATCCAAAGC TAGAAGATCC CAGGCGCCCT 
GACACCTCTT TCCTGTGGTT CACCTCACCA TACAAGACCA TGAAGTTCAT CTTGTGGCGA 
CGATTCCGGT GTGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT 
GTCTTTGTCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236831.1
CDS	397..6696
Translation	MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVAGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236769.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236769.3

ORF Insert Sequence:

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ATTCTGGGAA GAAGTGGCCA 
GTCCCCACGG ACACAGGAGG AGAGGAAGAC ACGGAAGACC AGGGCCTGAC TGGAGATGAA 
GCTGAGCTCT TCCCAGATCC GAGTGTTGCT GTGGGCCCAG GGGGACCAAC GACGCCCAAG 
AAGCCACCTT CCCATCCTCC TCCCCACTAT CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT 
CCACCTAGAA AGCTGCTGTC AGACAAGCCA CAGGACTTCC AGATCAGAGT CCAGGTGATT 
GAGGGGCGTC AGCTGCCTGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG 
CAGACCAAGC GGACTCGAAT CCAGAAGGGA AATAGCCCAC TTTTCAATGA GACTCTTTTC 
TTCAACGTAT TTGACTCTCC CTTGGAACTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCACGCT CCCTCAGGAC AGATGCCCTC CTTGGCGAGT TCCGGATGGA TGTGGGCACC 
GTGTACAGAG AGCCCCGACA TGCTTATCTC CGGAAGTGGC TGCTGCTCTC CGATCCCGAT 
GACTTCTCTG CAGGGGCCAG AGGCTACCTC AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG 
GACGAAGCTC CCCTAGACAA GAAAGACCCT TCTGAAGACA AAGAGGACAT TGAAGGCAAC 
CTGCTTAGGC CCACTGGTGT GGCCCTTCGT GGTGCCCATT TTTGCCTGAA GCTCTTCCGG 
GCTGAGGACT TACCACAGAT GGACGATGCT GTGATGGACA ACGTCAAGCA AATCTTTGGC 
TTTGATAGCA ACAAGAAGAA CCTGGTGGAC CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA 
ATGCTTTGCA GCAAGATCCT GGAGAAGACT GCTAACCCTC AGTGGAACCA GAACATCACA 
TTGCCTGCGA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACTC ACAATGACAC TGTGGCTACC ACCTACTTGG GTATGTCGAA AATCTCTGCC 
ACTGGAGGAG AAATAGAAGT GGATGACAAC CTGGGCTTCC TCCCCACTTT TGGGCCCTGC 
TATGTGAACC TCTATGGCAG TCCCCGAGAG TTCACGGGCT TCCCAGATCC TTATGCGGAG 
CTCAACACAG GAAAGGGAGA AGGTGTGGCA TATAGGGGCA GGCTTCTGTT GTCCCTGGAG 
ACCAAACTGG TGGAGCACAG TGAACAGAAG GTGGAAGACC TTCCTGCTGA TGACATCTTG 
AGAGTGGAGA AGTACCTGCG GAGGCGCAAA TATTCCCTCT TTGCTGCCTT CTACTCAGCC 
ACAATGCTGC AGGATGTGGA TGATGCCATC CAGTTCGAGG TCAGCATTGG GAACTACGGA 
AACAAGTTTG ACACCACCTG CTTGCCGTTG GCCTCTACCA CCCAATACAG CCGGGCAGTC 
TTTGATGGAT GCCACTACTA TTATTTGCCC TGGGGCAACG TGAAGCCTGT GGTAGTACTG 
TCCTCATACT GGGAAGACAT CAGCCATCGA GTCGAGACTC AGAACCAGCT CCTCAGGATC 
GCTGACCGAC TGGAAGCTAA CCTGGAGCAA GTCCACCTGG CTCTGAAGGC ACAGTGTTCC 
TCTGAGGATG TGGACGCCCT GGTGGCTCAG TTGACAGATG AGCTCCTTGC AGACTGCAGC 
CAGCCCCTGT GTGACATCCA TGAAATACCT TCTGCCACCC ACCTTGATCA GTACCTGTTC 
CGCCTCCGCT CCCGTCACCT GAGCCAGATC AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC 
CACAGTGAGC TCTCCACTGC CCTGGAGCAG GCCGAGGACT GGCTTCTGCA TCTCCGTGCC 
CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA GATATCATCA TCTGGATGCT GCAGGGTGAC 
AAGCGTGTGG CCTACCAACG GGTGCCTGCC CATGAAGTCC TCTTCTCCCG GCGGGGCCCC 
AGCTACTGCG GCAGGAACTG TGGGAATCTG CAGACCATCT TTCTGAAATA TCCTATGGAA 
GGCATGCCTG GGCCCCGGAT GCCGGTGCAG ATCCGGATCA AGCTCTGGTT TGGGCTCTCA 
GTGGATGAGA AGGAATTCAA TCAGTTTGCA GAGGGGAAGC TCTCTGTCTT TGCTGAAACG 
TATGAGAACC AGACAAAGCT GGCGCTGGTC GGGAACTGGG GCACGACGGG CCTCACCTAC 
CCCAAGTTTT CTGACGTCAC AGGCAAGATC AAACTACCGA AGGATAGCTT CCGCCCCTCA 
GCTGGCTGGG CCTGGGCAGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAT 
GCTGACGCCG GTCATCTGAG CTTTGTGGAG GAGGTGTTTG AGAACCAGAC TCGGCTACCT 
GGAGGCCAGT GGATCTATAT GAGTGACAAC TACACCGACG TGAATGGGGA GAAGGTGCTT 
CCAAAGGACG ATATTGAATG CCCGCTGGGT TGGAAGTGGG AAGATGAAGA ATGGTCCACA 
GACCTCAATC GGGCTGTTGA TGAACAAGGC TGGGAGTACA GCATCACCAT ACCCCCAGAC 
CGAAAGCCCA AACACTGGGT CCCTGTTGAG AAGATGTATT ACACTCACAG GCGTCGACGC 
TGGGTCCGAC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAA GCACAGGCAG 
GCAGAGGCTG AGGGCGAGGG CTGGGAATAC GCCTCTCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACAGATGC CTTTCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCACTG 
GAGAAGACGG GGCCTGCAGC GGTGTTTGCC CTTGAGGGGG CCCTGGGCGG CATGGTGGAT 
GACAAGAGCG AAGATTCCAT GTCTGTGTCT ACCCTGAGCT TTGGCGTTAA CAGACCCACA 
ATCTCCTGCA TCTTTGACTA TGGGAACCGC TACCATCTGC GTTGCTACCT GTACCAGGCC 
CGGGACCTGC CCGCCATGGA CAAAGACTCC TTCTCTGATC CCTATGCCAT TGTCTCCTTC 
CTGCATCAAA GCCAGAAGAC GGTGGTGGAG AAGAACACCC TGAACCCCAC CTGGGATCAA 
ACCCTCATCT TCTATGAGAT AGAGATCTTT GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTATG GCGCTGACGA GTTCATGGGC 
CGCTGTATTT GTCAGCCAAG CCTGGAACGG ATGCCCCGCC TGGCCTGGTT CCCACTGACC 
AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG GCAGCGTTCG AGCTTATCCA GAGAGAGAAG 
CCAGCCATCC ATCACATCCC TGGCTTTGAG ATGCACGAAA CTTCAAGGAT TTTGGATGAG 
ACGGAGGACA CAGACTTGCC TTACCCACCA CCTCAAAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCAGC TCTCCAGCGT ACAGCCATCG AGATCCTGGC CTGGGGCCTT 
CGGAACATAA AGAGCTATCA GATGGCCAGC GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT 
GGTGGGCAGA CAGTGCAGTC CTGTGTCATC AGAAACCTTC GGAAGAACCC CAACTTTGAC 
GTCTGTACTC TGTTCATGGA AGTGATGTTA CCCAGAGAGG ACCTCTACTG TCCCCCTATT 
GTGGTCAAGG TCATTGATAA TCGCCAGTTT GGCCGCAGGC CAGTTGTGGG TCAGTGCACC 
ATTCGCTCCC TGGAGAACTT CCTGTGTGAC CCGTACTCAG CAGAGAGTCC ATCCCCGCAA 
GGTGGCCCAG ATGACGTGAG CTTACTCAGC CCTGGGGAAG ACGTGCTCAT TGACATTGAT 
GACAAGGAGC CTCTCATCCC TGTCCAGGAG GAGGAGTTCA TTGACTGGTG GAGCAAATTC 
TTTGCTTCTA TCGGGGAGAG GGAAAAGTGT GGTTCCTACC TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGGAGGCCT GTCCGACTTT 
TGTAATACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG ACACAGAAGA TCCATCTGTC 
ATCGGAGAAT TTAAGGGTCT CTTCAAAATT TATCCCCTCC CAGAAGATCC AGCCATCCCT 
ATGCCCCCGA GACAATTCCA TCAGCTGGCT GCTCAGGGTC CCCAGGAATG CTTGGTCCGG 
ATTTACATCA TCCGAGCATT TGGCTTACAG CCCAAGGACC CCAATGGCAA GTGCGACCCT 
TACATCAAGA TCTCTATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCTTGT 
ACCCTGGAGC CTGTATTTGG AAAGATGTTT GAACTCACCT GCACTCTACC TCTGGAGAAG 
GACTTGAAGA TCACACTCTA TGACTATGAC CTGCTCTCCA AGGATGAGAA GATTGGGGAG 
ACAGTCATTG ATCTGGAGAA TAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGCCTTCCA 
CAGACCTACT GTGTCTCTGG ACCAAATCAG TGGAGAGATC AACTCCGCCC TTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCACAGGGTC AAGGCCCCCG TGTACCGGAC AGACCGAGTG 
ACGTTTCAGG ATAAGGAATA CACCATTCAG GAGATAGAGG CTGGCAGACT CCCAAACCCA 
CACCTGGGCC CGGTGGAGGA ACGCTTAGCC CTGCATGTCC TTCAACAACA AGGCTTGGTC 
CCTGAGCATG TGGAGTCTCG GCCTCTGTAC AGTCCTCTGC AGCCAGATAT TGAGCAGGGG 
AAGCTACAGA TGTGGATTGA CATATTTCCA AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAAAGC TCGAAGGTTT TTTCTGCGTT GTATTATTTG GAACACAAAA 
GATGTGATCT TGGATGACCT CAGCCTCACG GGGGAGAAGA TGAGTGACAT CTATGTGAAA 
GGCTGGATGG TGGGATTCGA AGAACACAAA CAGAAGACAG ATGTGCACTA CCGTTCCCTG 
GGCGGGGAGG GCAATTTCAA CTGGAGGTTC GTGTTTCCCT TCGACTATCT GCCCGCTGAG 
CAAGTCTGTG CTGTTGCCAA GAAGGATGCT TTCTGGAGAC TCGACAAGAC AGAGAGCAAG 
ATCCCAGCAC GTGTGGTCTT CCAGATTTGG GACAATGACA AGTTCTCCTT TGATGACTTT 
CTGGGCTCCC TGCAGCTGGA TCTCAACCGA ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACCTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAGACAG TGAAAGGATG GTGGCCTTGT GTGACAGAGG AGGGCGCGAA GAAGATCCTG 
GCGGGGAAGC TGGAAATGAC CTTGGAGATC GTTGCAGAAA GTGAACATGA GGAGCGGCCT 
GCTGGCCAGG GTCGGGATGA ACCCAACATG AATCCAAAGC TAGAAGATCC CAGGCGCCCT 
GACACCTCTT TCCTGTGGTT CACCTCACCA TACAAGACCA TGAAGTTCAT CTTGTGGCGA 
CGATTCCGGT GTGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT 
GTCTTTGTCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236770.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236832.1 Rattus norvegicus dysferlin (Dysf), transcript variant X16, mRNA.

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CloneID	ORa27411
Clone ID Related Accession (Same CDS sequence)	XM_006236770.3
Accession Version	XM_006236770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGT GGATGACAAC 
CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG 
TTCACGGGCT TCCCAGATCC TTATGCGGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCA 
TATAGGGGCA GGCTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAGCACAG TGAACAGAAG 
GTGGAAGACC TTCCTGCTGA TGACATCTTG AGAGTGGAGA AGTACCTGCG GAGGCGCAAA 
TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATTGG GAACTACGGA AACAAGTTTG ACACCACCTG CTTGCCGTTG 
GCCTCTACCA CCCAATACAG CCGGGCAGTC TTTGATGGAT GCCACTACTA TTATTTGCCC 
TGGGGCAACG TGAAGCCTGT GGTAGTACTG TCCTCATACT GGGAAGACAT CAGCCATCGA 
GTCGAGACTC AGAACCAGCT CCTCAGGATC GCTGACCGAC TGGAAGCTAA CCTGGAGCAA 
GTCCACCTGG CTCTGAAGGC ACAGTGTTCC TCTGAGGATG TGGACGCCCT GGTGGCTCAG 
TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA TGAAATACCT 
TCTGCCACCC ACCTTGATCA GTACCTGTTC CGCCTCCGCT CCCGTCACCT GAGCCAGATC 
AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC CACAGTGAGC TCTCCACTGC CCTGGAGCAG 
GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA 
GATATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAACG GGTGCCTGCC 
CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAATCTG 
CAGACCATCT TTCTGAAATA TCCTATGGAA GGCATGCCTG GGCCCCGGAT GCCGGTGCAG 
ATCCGGATCA AGCTCTGGTT TGGGCTCTCA GTGGATGAGA AGGAATTCAA TCAGTTTGCA 
GAGGGGAAGC TCTCTGTCTT TGCTGAAACG TATGAGAACC AGACAAAGCT GGCGCTGGTC 
GGGAACTGGG GCACGACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC 
AAACTACCGA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT GCTCCATGAT GCTGACGCCG GTCATCTGAG CTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC TCGGCTACCT GGAGGCCAGT GGATCTATAT GAGTGACAAC 
TACACCGACG TGAATGGGGA GAAGGTGCTT CCAAAGGACG ATATTGAATG CCCGCTGGGT 
TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCCA AACACTGGGT CCCTGTTGAG 
AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CACTGAAGAA GCACAGGCAG GCAGAGGCTG AGGGCGAGGG CTGGGAATAC 
GCCTCTCTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACGG GGCCTGCAGC GGTGTTTGCC 
CTTGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGATTCCAT GTCTGTGTCT 
ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTGC GTTGCTACCT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAAGACTCC 
TTCTCTGATC CCTATGCCAT TGTCTCCTTC CTGCATCAAA GCCAGAAGAC GGTGGTGGAG 
AAGAACACCC TGAACCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT AGAGATCTTT 
GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA CCCAGCATTG TGGTGGAGCT GTATGACCAT 
GACACCTATG GCGCTGACGA GTTCATGGGC CGCTGTATTT GTCAGCCAAG CCTGGAACGG 
ATGCCCCGCC TGGCCTGGTT CCCACTGACC AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG 
GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG 
ATGCACGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGACTTGCC TTACCCACCA 
CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCAGC TCTCCAGCGT 
ACAGCCATCG AGATCCTGGC CTGGGGCCTT CGGAACATAA AGAGCTATCA GATGGCCAGC 
GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC 
AGAAACCTTC GGAAGAACCC CAACTTTGAC GTCTGTACTC TGTTCATGGA AGTGATGTTA 
CCCAGAGAGG ACCTCTACTG TCCCCCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT 
GGCCGCAGGC CAGTTGTGGG TCAGTGCACC ATTCGCTCCC TGGAGAACTT CCTGTGTGAC 
CCGTACTCAG CAGAGAGTCC ATCCCCGCAA GGTGGCCCAG ATGACGTGAG CTTACTCAGC 
CCTGGGGAAG ACGTGCTCAT TGACATTGAT GACAAGGAGC CTCTCATCCC TGTCCAGCTT 
GCAGATGGGC TGTCAAGCTT GGGCCCCACT AACCTGATGC CTTCTCCGTC CAGTCCTCAT 
GAGGAGGAGT TCATTGACTG GTGGAGCAAA TTCTTTGCTT CTATCGGGGA GAGGGAAAAG 
TGTGGTTCCT ACCTGGAGAA GGATTTTGAC ACCCTGAAGG TCTATGACAC ACAGCTGGAG 
AATGTGGAGG CCTTTGGAGG CCTGTCCGAC TTTTGTAATA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGACACAGA AGATCCATCT GTCATCGGAG AATTTAAGGG TCTCTTCAAA 
ATTTATCCCC TCCCAGAAGA TCCAGCCATC CCTATGCCCC CGAGACAATT CCATCAGCTG 
GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC CGGATTTACA TCATCCGAGC ATTTGGCTTA 
CAGCCCAAGG ACCCCAATGG CAAGTGCGAC CCTTACATCA AGATCTCTAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATCCCT TGTACCCTGG AGCCTGTATT TGGAAAGATG 
TTTGAACTCA CCTGCACTCT ACCTCTGGAG AAGGACTTGA AGATCACACT CTATGACTAT 
GACCTGCTCT CCAAGGATGA GAAGATTGGG GAGACAGTCA TTGATCTGGA GAATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGTGGCCTT CCACAGACCT ACTGTGTCTC TGGACCAAAT 
CAGTGGAGAG ATCAACTCCG CCCTTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCACAGG 
GTCAAGGCCC CCGTGTACCG GACAGACCGA GTGACGTTTC AGGATAAGGA ATACACCATT 
CAGGAGATAG AGGCTGGCAG ACTCCCAAAC CCACACCTGG GCCCGGTGGA GGAACGCTTA 
GCCCTGCATG TCCTTCAACA ACAAGGCTTG GTCCCTGAGC ATGTGGAGTC TCGGCCTCTG 
TACAGTCCTC TGCAGCCAGA TATTGAGCAG GGGAAGCTAC AGATGTGGAT TGACATATTT 
CCAAAGGTGC TGGGCCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAA AGCTCGAAGG 
TTTTTTCTGC GTTGTATTAT TTGGAACACA AAAGATGTGA TCTTGGATGA CCTCAGCCTC 
ACGGGGGAGA AGATGAGTGA CATCTATGTG AAAGGCTGGA TGGTGGGATT CGAAGAACAC 
AAACAGAAGA CAGATGTGCA CTACCGTTCC CTGGGCGGGG AGGGCAATTT CAACTGGAGG 
TTCGTGTTTC CCTTCGACTA TCTGCCCGCT GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT 
GCTTTCTGGA GACTCGACAA GACAGAGAGC AAGATCCCAG CACGTGTGGT CTTCCAGATT 
TGGGACAATG ACAAGTTCTC CTTTGATGAC TTTCTGGGCT CCCTGCAGCT GGATCTCAAC 
CGAATGCCCA AGCCAGCCAA GACGGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACC 
TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT 
TGTGTGACAG AGGAGGGCGC GAAGAAGATC CTGGCGGGGA AGCTGGAAAT GACCTTGGAG 
ATCGTTGCAG AAAGTGAACA TGAGGAGCGG CCTGCTGGCC AGGGTCGGGA TGAACCCAAC 
ATGAATCCAA AGCTAGAAGA TCCCAGGCGC CCTGACACCT CTTTCCTGTG GTTCACCTCA 
CCATACAAGA CCATGAAGTT CATCTTGTGG CGACGATTCC GGTGTGCCAT CATCCTCTTC 
ATCATCCTCT TCATCCTCCT GCTCTTCCTG GGTGTCTTTG TCTACGCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTTAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236832.1
CDS	702..6974
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236770.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236770.3

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGT GGATGACAAC 
CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG 
TTCACGGGCT TCCCAGATCC TTATGCGGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCA 
TATAGGGGCA GGCTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAGCACAG TGAACAGAAG 
GTGGAAGACC TTCCTGCTGA TGACATCTTG AGAGTGGAGA AGTACCTGCG GAGGCGCAAA 
TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATTGG GAACTACGGA AACAAGTTTG ACACCACCTG CTTGCCGTTG 
GCCTCTACCA CCCAATACAG CCGGGCAGTC TTTGATGGAT GCCACTACTA TTATTTGCCC 
TGGGGCAACG TGAAGCCTGT GGTAGTACTG TCCTCATACT GGGAAGACAT CAGCCATCGA 
GTCGAGACTC AGAACCAGCT CCTCAGGATC GCTGACCGAC TGGAAGCTAA CCTGGAGCAA 
GTCCACCTGG CTCTGAAGGC ACAGTGTTCC TCTGAGGATG TGGACGCCCT GGTGGCTCAG 
TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA TGAAATACCT 
TCTGCCACCC ACCTTGATCA GTACCTGTTC CGCCTCCGCT CCCGTCACCT GAGCCAGATC 
AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC CACAGTGAGC TCTCCACTGC CCTGGAGCAG 
GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA 
GATATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAACG GGTGCCTGCC 
CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAATCTG 
CAGACCATCT TTCTGAAATA TCCTATGGAA GGCATGCCTG GGCCCCGGAT GCCGGTGCAG 
ATCCGGATCA AGCTCTGGTT TGGGCTCTCA GTGGATGAGA AGGAATTCAA TCAGTTTGCA 
GAGGGGAAGC TCTCTGTCTT TGCTGAAACG TATGAGAACC AGACAAAGCT GGCGCTGGTC 
GGGAACTGGG GCACGACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC 
AAACTACCGA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT GCTCCATGAT GCTGACGCCG GTCATCTGAG CTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC TCGGCTACCT GGAGGCCAGT GGATCTATAT GAGTGACAAC 
TACACCGACG TGAATGGGGA GAAGGTGCTT CCAAAGGACG ATATTGAATG CCCGCTGGGT 
TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCCA AACACTGGGT CCCTGTTGAG 
AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CACTGAAGAA GCACAGGCAG GCAGAGGCTG AGGGCGAGGG CTGGGAATAC 
GCCTCTCTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACGG GGCCTGCAGC GGTGTTTGCC 
CTTGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGATTCCAT GTCTGTGTCT 
ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTGC GTTGCTACCT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAAGACTCC 
TTCTCTGATC CCTATGCCAT TGTCTCCTTC CTGCATCAAA GCCAGAAGAC GGTGGTGGAG 
AAGAACACCC TGAACCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT AGAGATCTTT 
GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA CCCAGCATTG TGGTGGAGCT GTATGACCAT 
GACACCTATG GCGCTGACGA GTTCATGGGC CGCTGTATTT GTCAGCCAAG CCTGGAACGG 
ATGCCCCGCC TGGCCTGGTT CCCACTGACC AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG 
GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG 
ATGCACGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGACTTGCC TTACCCACCA 
CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCAGC TCTCCAGCGT 
ACAGCCATCG AGATCCTGGC CTGGGGCCTT CGGAACATAA AGAGCTATCA GATGGCCAGC 
GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC 
AGAAACCTTC GGAAGAACCC CAACTTTGAC GTCTGTACTC TGTTCATGGA AGTGATGTTA 
CCCAGAGAGG ACCTCTACTG TCCCCCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT 
GGCCGCAGGC CAGTTGTGGG TCAGTGCACC ATTCGCTCCC TGGAGAACTT CCTGTGTGAC 
CCGTACTCAG CAGAGAGTCC ATCCCCGCAA GGTGGCCCAG ATGACGTGAG CTTACTCAGC 
CCTGGGGAAG ACGTGCTCAT TGACATTGAT GACAAGGAGC CTCTCATCCC TGTCCAGCTT 
GCAGATGGGC TGTCAAGCTT GGGCCCCACT AACCTGATGC CTTCTCCGTC CAGTCCTCAT 
GAGGAGGAGT TCATTGACTG GTGGAGCAAA TTCTTTGCTT CTATCGGGGA GAGGGAAAAG 
TGTGGTTCCT ACCTGGAGAA GGATTTTGAC ACCCTGAAGG TCTATGACAC ACAGCTGGAG 
AATGTGGAGG CCTTTGGAGG CCTGTCCGAC TTTTGTAATA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGACACAGA AGATCCATCT GTCATCGGAG AATTTAAGGG TCTCTTCAAA 
ATTTATCCCC TCCCAGAAGA TCCAGCCATC CCTATGCCCC CGAGACAATT CCATCAGCTG 
GCTGCTCAGG GTCCCCAGGA ATGCTTGGTC CGGATTTACA TCATCCGAGC ATTTGGCTTA 
CAGCCCAAGG ACCCCAATGG CAAGTGCGAC CCTTACATCA AGATCTCTAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATCCCT TGTACCCTGG AGCCTGTATT TGGAAAGATG 
TTTGAACTCA CCTGCACTCT ACCTCTGGAG AAGGACTTGA AGATCACACT CTATGACTAT 
GACCTGCTCT CCAAGGATGA GAAGATTGGG GAGACAGTCA TTGATCTGGA GAATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGTGGCCTT CCACAGACCT ACTGTGTCTC TGGACCAAAT 
CAGTGGAGAG ATCAACTCCG CCCTTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCACAGG 
GTCAAGGCCC CCGTGTACCG GACAGACCGA GTGACGTTTC AGGATAAGGA ATACACCATT 
CAGGAGATAG AGGCTGGCAG ACTCCCAAAC CCACACCTGG GCCCGGTGGA GGAACGCTTA 
GCCCTGCATG TCCTTCAACA ACAAGGCTTG GTCCCTGAGC ATGTGGAGTC TCGGCCTCTG 
TACAGTCCTC TGCAGCCAGA TATTGAGCAG GGGAAGCTAC AGATGTGGAT TGACATATTT 
CCAAAGGTGC TGGGCCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAA AGCTCGAAGG 
TTTTTTCTGC GTTGTATTAT TTGGAACACA AAAGATGTGA TCTTGGATGA CCTCAGCCTC 
ACGGGGGAGA AGATGAGTGA CATCTATGTG AAAGGCTGGA TGGTGGGATT CGAAGAACAC 
AAACAGAAGA CAGATGTGCA CTACCGTTCC CTGGGCGGGG AGGGCAATTT CAACTGGAGG 
TTCGTGTTTC CCTTCGACTA TCTGCCCGCT GAGCAAGTCT GTGCTGTTGC CAAGAAGGAT 
GCTTTCTGGA GACTCGACAA GACAGAGAGC AAGATCCCAG CACGTGTGGT CTTCCAGATT 
TGGGACAATG ACAAGTTCTC CTTTGATGAC TTTCTGGGCT CCCTGCAGCT GGATCTCAAC 
CGAATGCCCA AGCCAGCCAA GACGGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACC 
TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAGA CAGTGAAAGG ATGGTGGCCT 
TGTGTGACAG AGGAGGGCGC GAAGAAGATC CTGGCGGGGA AGCTGGAAAT GACCTTGGAG 
ATCGTTGCAG AAAGTGAACA TGAGGAGCGG CCTGCTGGCC AGGGTCGGGA TGAACCCAAC 
ATGAATCCAA AGCTAGAAGA TCCCAGGCGC CCTGACACCT CTTTCCTGTG GTTCACCTCA 
CCATACAAGA CCATGAAGTT CATCTTGTGG CGACGATTCC GGTGTGCCAT CATCCTCTTC 
ATCATCCTCT TCATCCTCCT GCTCTTCCTG GGTGTCTTTG TCTACGCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTTAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236771.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236833.1 Rattus norvegicus dysferlin (Dysf), transcript variant X17, mRNA.

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CloneID	ORa27412
Clone ID Related Accession (Same CDS sequence)	XM_006236771.3
Accession Version	XM_006236771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGTGGA TGACAACCTG 
GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC 
ACGGGCTTCC CAGATCCTTA TGCGGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCATAT 
AGGGGCAGGC TTCTGTTGTC CCTGGAGACC AAACTGGTGG AGCACAGTGA ACAGAAGGTG 
GAAGACCTTC CTGCTGATGA CATCTTGAGA GTGGAGAAGT ACCTGCGGAG GCGCAAATAT 
TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA TGCCATCCAG 
TTCGAGGTCA GCATTGGGAA CTACGGAAAC AAGTTTGACA CCACCTGCTT GCCGTTGGCC 
TCTACCACCC AATACAGCCG GGCAGTCTTT GATGGATGCC ACTACTATTA TTTGCCCTGG 
GGCAACGTGA AGCCTGTGGT AGTACTGTCC TCATACTGGG AAGACATCAG CCATCGAGTC 
GAGACTCAGA ACCAGCTCCT CAGGATCGCT GACCGACTGG AAGCTAACCT GGAGCAAGTC 
CACCTGGCTC TGAAGGCACA GTGTTCCTCT GAGGATGTGG ACGCCCTGGT GGCTCAGTTG 
ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCATGA AATACCTTCT 
GCCACCCACC TTGATCAGTA CCTGTTCCGC CTCCGCTCCC GTCACCTGAG CCAGATCAAA 
GAAGCTGCCC TGGCCTTGAA GCTTGGCCAC AGTGAGCTCT CCACTGCCCT GGAGCAGGCC 
GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCTGAGGAGC CCCAGAACAG CCTGCCAGAT 
ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAACGGGT GCCTGCCCAT 
GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAATCTGCAG 
ACCATCTTTC TGAAATATCC TATGGAAGGC ATGCCTGGGC CCCGGATGCC GGTGCAGATC 
CGGATCAAGC TCTGGTTTGG GCTCTCAGTG GATGAGAAGG AATTCAATCA GTTTGCAGAG 
GGGAAGCTCT CTGTCTTTGC TGAAACGTAT GAGAACCAGA CAAAGCTGGC GCTGGTCGGG 
AACTGGGGCA CGACGGGCCT CACCTACCCC AAGTTTTCTG ACGTCACAGG CAAGATCAAA 
CTACCGAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACCCTGCT CCATGATGCT GACGCCGGTC ATCTGAGCTT TGTGGAGGAG 
GTGTTTGAGA ACCAGACTCG GCTACCTGGA GGCCAGTGGA TCTATATGAG TGACAACTAC 
ACCGACGTGA ATGGGGAGAA GGTGCTTCCA AAGGACGATA TTGAATGCCC GCTGGGTTGG 
AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCCAAAC ACTGGGTCCC TGTTGAGAAG 
ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCAC TGAAGAAGCA CAGGCAGGCA GAGGCTGAGG GCGAGGGCTG GGAATACGCC 
TCTCTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT TCGCCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCACTGGAG AAGACGGGGC CTGCAGCGGT GTTTGCCCTT 
GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ATTCCATGTC TGTGTCTACC 
CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC 
CATCTGCGTT GCTACCTGTA CCAGGCCCGG GACCTGCCCG CCATGGACAA AGACTCCTTC 
TCTGATCCCT ATGCCATTGT CTCCTTCCTG CATCAAAGCC AGAAGACGGT GGTGGAGAAG 
AACACCCTGA ACCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATAGA GATCTTTGGC 
GAGCCGGCCA GTGTCGCCGA GCGGCCACCC AGCATTGTGG TGGAGCTGTA TGACCATGAC 
ACCTATGGCG CTGACGAGTT CATGGGCCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG 
CCCCGCCTGG CCTGGTTCCC ACTGACCAGG GGCAGCCAGC CTGCCGGAGA GCTGCTGGCA 
GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG 
CACGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ACTTGCCTTA CCCACCACCT 
CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCAGCTCT CCAGCGTACA 
GCCATCGAGA TCCTGGCCTG GGGCCTTCGG AACATAAAGA GCTATCAGAT GGCCAGCGTG 
TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA 
AACCTTCGGA AGAACCCCAA CTTTGACGTC TGTACTCTGT TCATGGAAGT GATGTTACCC 
AGAGAGGACC TCTACTGTCC CCCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC 
CGCAGGCCAG TTGTGGGTCA GTGCACCATT CGCTCCCTGG AGAACTTCCT GTGTGACCCG 
TACTCAGCAG AGAGTCCATC CCCGCAAGGT GGCCCAGATG ACGTGAGCTT ACTCAGCCCT 
GGGGAAGACG TGCTCATTGA CATTGATGAC AAGGAGCCTC TCATCCCTGT CCAGCTTGCA 
GATGGGCTGT CAAGCTTGGG CCCCACTAAC CTGATGCCTT CTCCGTCCAG TCCTCATGAG 
GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTA TCGGGGAGAG GGAAAAGTGT 
GGTTCCTACC TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGGAGGCCT GTCCGACTTT TGTAATACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG ACACAGAAGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT 
TATCCCCTCC CAGAAGATCC AGCCATCCCT ATGCCCCCGA GACAATTCCA TCAGCTGGCT 
GCTCAGGGTC CCCAGGAATG CTTGGTCCGG ATTTACATCA TCCGAGCATT TGGCTTACAG 
CCCAAGGACC CCAATGGCAA GTGCGACCCT TACATCAAGA TCTCTATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCTTGT ACCCTGGAGC CTGTATTTGG AAAGATGTTT 
GAACTCACCT GCACTCTACC TCTGGAGAAG GACTTGAAGA TCACACTCTA TGACTATGAC 
CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACAGTCATTG ATCTGGAGAA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGCCTTCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG 
TGGAGAGATC AACTCCGCCC TTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGAATA CACCATTCAG 
GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CGGTGGAGGA ACGCTTAGCC 
CTGCATGTCC TTCAACAACA AGGCTTGGTC CCTGAGCATG TGGAGTCTCG GCCTCTGTAC 
AGTCCTCTGC AGCCAGATAT TGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA 
AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TCGAAGGTTT 
TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGCCTCACG 
GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAA 
CAGAAGACAG ATGTGCACTA CCGTTCCCTG GGCGGGGAGG GCAATTTCAA CTGGAGGTTC 
GTGTTTCCCT TCGACTATCT GCCCGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCT 
TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATTTGG 
GACAATGACA AGTTCTCCTT TGATGACTTT CTGGGCTCCC TGCAGCTGGA TCTCAACCGA 
ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT 
GTGACAGAGG AGGGCGCGAA GAAGATCCTG GCGGGGAAGC TGGAAATGAC CTTGGAGATC 
GTTGCAGAAA GTGAACATGA GGAGCGGCCT GCTGGCCAGG GTCGGGATGA ACCCAACATG 
AATCCAAAGC TAGAAGATCC CAGGCGCCCT GACACCTCTT TCCTGTGGTT CACCTCACCA 
TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGATTCCGGT GTGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236833.1
CDS	397..6666
Translation	MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQLADGLSSLGPTNLMPSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236771.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236771.3

ORF Insert Sequence:

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGTGGA TGACAACCTG 
GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC 
ACGGGCTTCC CAGATCCTTA TGCGGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCATAT 
AGGGGCAGGC TTCTGTTGTC CCTGGAGACC AAACTGGTGG AGCACAGTGA ACAGAAGGTG 
GAAGACCTTC CTGCTGATGA CATCTTGAGA GTGGAGAAGT ACCTGCGGAG GCGCAAATAT 
TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA TGCCATCCAG 
TTCGAGGTCA GCATTGGGAA CTACGGAAAC AAGTTTGACA CCACCTGCTT GCCGTTGGCC 
TCTACCACCC AATACAGCCG GGCAGTCTTT GATGGATGCC ACTACTATTA TTTGCCCTGG 
GGCAACGTGA AGCCTGTGGT AGTACTGTCC TCATACTGGG AAGACATCAG CCATCGAGTC 
GAGACTCAGA ACCAGCTCCT CAGGATCGCT GACCGACTGG AAGCTAACCT GGAGCAAGTC 
CACCTGGCTC TGAAGGCACA GTGTTCCTCT GAGGATGTGG ACGCCCTGGT GGCTCAGTTG 
ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCATGA AATACCTTCT 
GCCACCCACC TTGATCAGTA CCTGTTCCGC CTCCGCTCCC GTCACCTGAG CCAGATCAAA 
GAAGCTGCCC TGGCCTTGAA GCTTGGCCAC AGTGAGCTCT CCACTGCCCT GGAGCAGGCC 
GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCTGAGGAGC CCCAGAACAG CCTGCCAGAT 
ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAACGGGT GCCTGCCCAT 
GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAATCTGCAG 
ACCATCTTTC TGAAATATCC TATGGAAGGC ATGCCTGGGC CCCGGATGCC GGTGCAGATC 
CGGATCAAGC TCTGGTTTGG GCTCTCAGTG GATGAGAAGG AATTCAATCA GTTTGCAGAG 
GGGAAGCTCT CTGTCTTTGC TGAAACGTAT GAGAACCAGA CAAAGCTGGC GCTGGTCGGG 
AACTGGGGCA CGACGGGCCT CACCTACCCC AAGTTTTCTG ACGTCACAGG CAAGATCAAA 
CTACCGAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACCCTGCT CCATGATGCT GACGCCGGTC ATCTGAGCTT TGTGGAGGAG 
GTGTTTGAGA ACCAGACTCG GCTACCTGGA GGCCAGTGGA TCTATATGAG TGACAACTAC 
ACCGACGTGA ATGGGGAGAA GGTGCTTCCA AAGGACGATA TTGAATGCCC GCTGGGTTGG 
AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCCAAAC ACTGGGTCCC TGTTGAGAAG 
ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCAC TGAAGAAGCA CAGGCAGGCA GAGGCTGAGG GCGAGGGCTG GGAATACGCC 
TCTCTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT TCGCCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCACTGGAG AAGACGGGGC CTGCAGCGGT GTTTGCCCTT 
GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ATTCCATGTC TGTGTCTACC 
CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC 
CATCTGCGTT GCTACCTGTA CCAGGCCCGG GACCTGCCCG CCATGGACAA AGACTCCTTC 
TCTGATCCCT ATGCCATTGT CTCCTTCCTG CATCAAAGCC AGAAGACGGT GGTGGAGAAG 
AACACCCTGA ACCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATAGA GATCTTTGGC 
GAGCCGGCCA GTGTCGCCGA GCGGCCACCC AGCATTGTGG TGGAGCTGTA TGACCATGAC 
ACCTATGGCG CTGACGAGTT CATGGGCCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG 
CCCCGCCTGG CCTGGTTCCC ACTGACCAGG GGCAGCCAGC CTGCCGGAGA GCTGCTGGCA 
GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG 
CACGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ACTTGCCTTA CCCACCACCT 
CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCAGCTCT CCAGCGTACA 
GCCATCGAGA TCCTGGCCTG GGGCCTTCGG AACATAAAGA GCTATCAGAT GGCCAGCGTG 
TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA 
AACCTTCGGA AGAACCCCAA CTTTGACGTC TGTACTCTGT TCATGGAAGT GATGTTACCC 
AGAGAGGACC TCTACTGTCC CCCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC 
CGCAGGCCAG TTGTGGGTCA GTGCACCATT CGCTCCCTGG AGAACTTCCT GTGTGACCCG 
TACTCAGCAG AGAGTCCATC CCCGCAAGGT GGCCCAGATG ACGTGAGCTT ACTCAGCCCT 
GGGGAAGACG TGCTCATTGA CATTGATGAC AAGGAGCCTC TCATCCCTGT CCAGCTTGCA 
GATGGGCTGT CAAGCTTGGG CCCCACTAAC CTGATGCCTT CTCCGTCCAG TCCTCATGAG 
GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTA TCGGGGAGAG GGAAAAGTGT 
GGTTCCTACC TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGGAGGCCT GTCCGACTTT TGTAATACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG ACACAGAAGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT 
TATCCCCTCC CAGAAGATCC AGCCATCCCT ATGCCCCCGA GACAATTCCA TCAGCTGGCT 
GCTCAGGGTC CCCAGGAATG CTTGGTCCGG ATTTACATCA TCCGAGCATT TGGCTTACAG 
CCCAAGGACC CCAATGGCAA GTGCGACCCT TACATCAAGA TCTCTATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCTTGT ACCCTGGAGC CTGTATTTGG AAAGATGTTT 
GAACTCACCT GCACTCTACC TCTGGAGAAG GACTTGAAGA TCACACTCTA TGACTATGAC 
CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACAGTCATTG ATCTGGAGAA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGCCTTCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG 
TGGAGAGATC AACTCCGCCC TTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGAATA CACCATTCAG 
GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CGGTGGAGGA ACGCTTAGCC 
CTGCATGTCC TTCAACAACA AGGCTTGGTC CCTGAGCATG TGGAGTCTCG GCCTCTGTAC 
AGTCCTCTGC AGCCAGATAT TGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA 
AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TCGAAGGTTT 
TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGCCTCACG 
GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAA 
CAGAAGACAG ATGTGCACTA CCGTTCCCTG GGCGGGGAGG GCAATTTCAA CTGGAGGTTC 
GTGTTTCCCT TCGACTATCT GCCCGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCT 
TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATTTGG 
GACAATGACA AGTTCTCCTT TGATGACTTT CTGGGCTCCC TGCAGCTGGA TCTCAACCGA 
ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT 
GTGACAGAGG AGGGCGCGAA GAAGATCCTG GCGGGGAAGC TGGAAATGAC CTTGGAGATC 
GTTGCAGAAA GTGAACATGA GGAGCGGCCT GCTGGCCAGG GTCGGGATGA ACCCAACATG 
AATCCAAAGC TAGAAGATCC CAGGCGCCCT GACACCTCTT TCCTGTGGTT CACCTCACCA 
TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGATTCCGGT GTGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236772.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236834.1 Rattus norvegicus dysferlin (Dysf), transcript variant X18, mRNA.

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CloneID	ORa27413
Clone ID Related Accession (Same CDS sequence)	XM_006236772.3
Accession Version	XM_006236772.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236772.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTACTCA AGAGTCCAGC CACTGACCTG GATGACAACC TGGGCTTCCT CCCCACTTTT 
GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT 
TATGCGGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG 
TCCCTGGAGA CCAAACTGGT GGAGCACAGT GAACAGAAGG TGGAAGACCT TCCTGCTGAT 
GACATCTTGA GAGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC 
TACTCAGCCA CAATGCTGCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATTGGG 
AACTACGGAA ACAAGTTTGA CACCACCTGC TTGCCGTTGG CCTCTACCAC CCAATACAGC 
CGGGCAGTCT TTGATGGATG CCACTACTAT TATTTGCCCT GGGGCAACGT GAAGCCTGTG 
GTAGTACTGT CCTCATACTG GGAAGACATC AGCCATCGAG TCGAGACTCA GAACCAGCTC 
CTCAGGATCG CTGACCGACT GGAAGCTAAC CTGGAGCAAG TCCACCTGGC TCTGAAGGCA 
CAGTGTTCCT CTGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA 
GACTGCAGCC AGCCCCTGTG TGACATCCAT GAAATACCTT CTGCCACCCA CCTTGATCAG 
TACCTGTTCC GCCTCCGCTC CCGTCACCTG AGCCAGATCA AAGAAGCTGC CCTGGCCTTG 
AAGCTTGGCC ACAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT 
CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC AGCCTGCCAG ATATCATCAT CTGGATGCTG 
CAGGGTGACA AGCGTGTGGC CTACCAACGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG 
CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAATCTGC AGACCATCTT TCTGAAATAT 
CCTATGGAAG GCATGCCTGG GCCCCGGATG CCGGTGCAGA TCCGGATCAA GCTCTGGTTT 
GGGCTCTCAG TGGATGAGAA GGAATTCAAT CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT 
GCTGAAACGT ATGAGAACCA GACAAAGCTG GCGCTGGTCG GGAACTGGGG CACGACGGGC 
CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AACTACCGAA GGATAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGATG CTGACGCCGG TCATCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACT 
CGGCTACCTG GAGGCCAGTG GATCTATATG AGTGACAACT ACACCGACGT GAATGGGGAG 
AAGGTGCTTC CAAAGGACGA TATTGAATGC CCGCTGGGTT GGAAGTGGGA AGATGAAGAA 
TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA 
CCCCCAGACC GAAAGCCCAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG 
CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAAG 
CACAGGCAGG CAGAGGCTGA GGGCGAGGGC TGGGAATACG CCTCTCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGATGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCACTGG AGAAGACGGG GCCTGCAGCG GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC 
ATGGTGGATG ACAAGAGCGA AGATTCCATG TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC 
AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG TTGCTACCTG 
TACCAGGCCC GGGACCTGCC CGCCATGGAC AAAGACTCCT TCTCTGATCC CTATGCCATT 
GTCTCCTTCC TGCATCAAAG CCAGAAGACG GTGGTGGAGA AGAACACCCT GAACCCCACC 
TGGGATCAAA CCCTCATCTT CTATGAGATA GAGATCTTTG GCGAGCCGGC CAGTGTCGCC 
GAGCGGCCAC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTATGG CGCTGACGAG 
TTCATGGGCC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC 
CCACTGACCA GGGGCAGCCA GCCTGCCGGA GAGCTGCTGG CAGCGTTCGA GCTTATCCAG 
AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCACGAAAC TTCAAGGATT 
TTGGATGAGA CGGAGGACAC AGACTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGCT CTCCAGCGTA CAGCCATCGA GATCCTGGCC 
TGGGGCCTTC GGAACATAAA GAGCTATCAG ATGGCCAGCG TGTCTTCCCC CAGCCTCGTG 
GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC 
AACTTTGACG TCTGTACTCT GTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT 
CCCCCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCAGGCC AGTTGTGGGT 
CAGTGCACCA TTCGCTCCCT GGAGAACTTC CTGTGTGACC CGTACTCAGC AGAGAGTCCA 
TCCCCGCAAG GTGGCCCAGA TGACGTGAGC TTACTCAGCC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ACAAGGAGCC TCTCATCCCT GTCCAGGAGG AGGAGTTCAT TGACTGGTGG 
AGCAAATTCT TTGCTTCTAT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGGAGGCCTG 
TCCGACTTTT GTAATACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA CACAGAAGAT 
CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA 
GCCATCCCTA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC 
TTGGTCCGGA TTTACATCAT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG 
TGCGACCCTT ACATCAAGAT CTCTATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCTTGTA CCCTGGAGCC TGTATTTGGA AAGATGTTTG AACTCACCTG CACTCTACCT 
CTGGAGAAGG ACTTGAAGAT CACACTCTAT GACTATGACC TGCTCTCCAA GGATGAGAAG 
ATTGGGGAGA CAGTCATTGA TCTGGAGAAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGCCTTCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA ACTCCGCCCT 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGGTCA AGGCCCCCGT GTACCGGACA 
GACCGAGTGA CGTTTCAGGA TAAGGAATAC ACCATTCAGG AGATAGAGGC TGGCAGACTC 
CCAAACCCAC ACCTGGGCCC GGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAACAACAA 
GGCTTGGTCC CTGAGCATGT GGAGTCTCGG CCTCTGTACA GTCCTCTGCA GCCAGATATT 
GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAAAGCT CGAAGGTTTT TTCTGCGTTG TATTATTTGG 
AACACAAAAG ATGTGATCTT GGATGACCTC AGCCTCACGG GGGAGAAGAT GAGTGACATC 
TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAAC AGAAGACAGA TGTGCACTAC 
CGTTCCCTGG GCGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG 
CCCGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCTT TCTGGAGACT CGACAAGACA 
GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATTTGGG ACAATGACAA GTTCTCCTTT 
GATGACTTTC TGGGCTCCCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGCGAAG 
AAGATCCTGG CGGGGAAGCT GGAAATGACC TTGGAGATCG TTGCAGAAAG TGAACATGAG 
GAGCGGCCTG CTGGCCAGGG TCGGGATGAA CCCAACATGA ATCCAAAGCT AGAAGATCCC 
AGGCGCCCTG ACACCTCTTT CCTGTGGTTC ACCTCACCAT ACAAGACCAT GAAGTTCATC 
TTGTGGCGAC GATTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC 
TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236834.1
CDS	702..6950
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236772.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236772.3

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTACTCA AGAGTCCAGC CACTGACCTG GATGACAACC TGGGCTTCCT CCCCACTTTT 
GGGCCCTGCT ATGTGAACCT CTATGGCAGT CCCCGAGAGT TCACGGGCTT CCCAGATCCT 
TATGCGGAGC TCAACACAGG AAAGGGAGAA GGTGTGGCAT ATAGGGGCAG GCTTCTGTTG 
TCCCTGGAGA CCAAACTGGT GGAGCACAGT GAACAGAAGG TGGAAGACCT TCCTGCTGAT 
GACATCTTGA GAGTGGAGAA GTACCTGCGG AGGCGCAAAT ATTCCCTCTT TGCTGCCTTC 
TACTCAGCCA CAATGCTGCA GGATGTGGAT GATGCCATCC AGTTCGAGGT CAGCATTGGG 
AACTACGGAA ACAAGTTTGA CACCACCTGC TTGCCGTTGG CCTCTACCAC CCAATACAGC 
CGGGCAGTCT TTGATGGATG CCACTACTAT TATTTGCCCT GGGGCAACGT GAAGCCTGTG 
GTAGTACTGT CCTCATACTG GGAAGACATC AGCCATCGAG TCGAGACTCA GAACCAGCTC 
CTCAGGATCG CTGACCGACT GGAAGCTAAC CTGGAGCAAG TCCACCTGGC TCTGAAGGCA 
CAGTGTTCCT CTGAGGATGT GGACGCCCTG GTGGCTCAGT TGACAGATGA GCTCCTTGCA 
GACTGCAGCC AGCCCCTGTG TGACATCCAT GAAATACCTT CTGCCACCCA CCTTGATCAG 
TACCTGTTCC GCCTCCGCTC CCGTCACCTG AGCCAGATCA AAGAAGCTGC CCTGGCCTTG 
AAGCTTGGCC ACAGTGAGCT CTCCACTGCC CTGGAGCAGG CCGAGGACTG GCTTCTGCAT 
CTCCGTGCCC TGGCTGAGGA GCCCCAGAAC AGCCTGCCAG ATATCATCAT CTGGATGCTG 
CAGGGTGACA AGCGTGTGGC CTACCAACGG GTGCCTGCCC ATGAAGTCCT CTTCTCCCGG 
CGGGGCCCCA GCTACTGCGG CAGGAACTGT GGGAATCTGC AGACCATCTT TCTGAAATAT 
CCTATGGAAG GCATGCCTGG GCCCCGGATG CCGGTGCAGA TCCGGATCAA GCTCTGGTTT 
GGGCTCTCAG TGGATGAGAA GGAATTCAAT CAGTTTGCAG AGGGGAAGCT CTCTGTCTTT 
GCTGAAACGT ATGAGAACCA GACAAAGCTG GCGCTGGTCG GGAACTGGGG CACGACGGGC 
CTCACCTACC CCAAGTTTTC TGACGTCACA GGCAAGATCA AACTACCGAA GGATAGCTTC 
CGCCCCTCAG CTGGCTGGGC CTGGGCAGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGATG CTGACGCCGG TCATCTGAGC TTTGTGGAGG AGGTGTTTGA GAACCAGACT 
CGGCTACCTG GAGGCCAGTG GATCTATATG AGTGACAACT ACACCGACGT GAATGGGGAG 
AAGGTGCTTC CAAAGGACGA TATTGAATGC CCGCTGGGTT GGAAGTGGGA AGATGAAGAA 
TGGTCCACAG ACCTCAATCG GGCTGTTGAT GAACAAGGCT GGGAGTACAG CATCACCATA 
CCCCCAGACC GAAAGCCCAA ACACTGGGTC CCTGTTGAGA AGATGTATTA CACTCACAGG 
CGTCGACGCT GGGTCCGACT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAAG 
CACAGGCAGG CAGAGGCTGA GGGCGAGGGC TGGGAATACG CCTCTCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGATGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCACTGG AGAAGACGGG GCCTGCAGCG GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC 
ATGGTGGATG ACAAGAGCGA AGATTCCATG TCTGTGTCTA CCCTGAGCTT TGGCGTTAAC 
AGACCCACAA TCTCCTGCAT CTTTGACTAT GGGAACCGCT ACCATCTGCG TTGCTACCTG 
TACCAGGCCC GGGACCTGCC CGCCATGGAC AAAGACTCCT TCTCTGATCC CTATGCCATT 
GTCTCCTTCC TGCATCAAAG CCAGAAGACG GTGGTGGAGA AGAACACCCT GAACCCCACC 
TGGGATCAAA CCCTCATCTT CTATGAGATA GAGATCTTTG GCGAGCCGGC CAGTGTCGCC 
GAGCGGCCAC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTATGG CGCTGACGAG 
TTCATGGGCC GCTGTATTTG TCAGCCAAGC CTGGAACGGA TGCCCCGCCT GGCCTGGTTC 
CCACTGACCA GGGGCAGCCA GCCTGCCGGA GAGCTGCTGG CAGCGTTCGA GCTTATCCAG 
AGAGAGAAGC CAGCCATCCA TCACATCCCT GGCTTTGAGA TGCACGAAAC TTCAAGGATT 
TTGGATGAGA CGGAGGACAC AGACTTGCCT TACCCACCAC CTCAAAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGCT CTCCAGCGTA CAGCCATCGA GATCCTGGCC 
TGGGGCCTTC GGAACATAAA GAGCTATCAG ATGGCCAGCG TGTCTTCCCC CAGCCTCGTG 
GTGGAGTGTG GTGGGCAGAC AGTGCAGTCC TGTGTCATCA GAAACCTTCG GAAGAACCCC 
AACTTTGACG TCTGTACTCT GTTCATGGAA GTGATGTTAC CCAGAGAGGA CCTCTACTGT 
CCCCCTATTG TGGTCAAGGT CATTGATAAT CGCCAGTTTG GCCGCAGGCC AGTTGTGGGT 
CAGTGCACCA TTCGCTCCCT GGAGAACTTC CTGTGTGACC CGTACTCAGC AGAGAGTCCA 
TCCCCGCAAG GTGGCCCAGA TGACGTGAGC TTACTCAGCC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ACAAGGAGCC TCTCATCCCT GTCCAGGAGG AGGAGTTCAT TGACTGGTGG 
AGCAAATTCT TTGCTTCTAT CGGGGAGAGG GAAAAGTGTG GTTCCTACCT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGGAGGCCTG 
TCCGACTTTT GTAATACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA CACAGAAGAT 
CCATCTGTCA TCGGAGAATT TAAGGGTCTC TTCAAAATTT ATCCCCTCCC AGAAGATCCA 
GCCATCCCTA TGCCCCCGAG ACAATTCCAT CAGCTGGCTG CTCAGGGTCC CCAGGAATGC 
TTGGTCCGGA TTTACATCAT CCGAGCATTT GGCTTACAGC CCAAGGACCC CAATGGCAAG 
TGCGACCCTT ACATCAAGAT CTCTATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCTTGTA CCCTGGAGCC TGTATTTGGA AAGATGTTTG AACTCACCTG CACTCTACCT 
CTGGAGAAGG ACTTGAAGAT CACACTCTAT GACTATGACC TGCTCTCCAA GGATGAGAAG 
ATTGGGGAGA CAGTCATTGA TCTGGAGAAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGCCTTCCAC AGACCTACTG TGTCTCTGGA CCAAATCAGT GGAGAGATCA ACTCCGCCCT 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CACAGGGTCA AGGCCCCCGT GTACCGGACA 
GACCGAGTGA CGTTTCAGGA TAAGGAATAC ACCATTCAGG AGATAGAGGC TGGCAGACTC 
CCAAACCCAC ACCTGGGCCC GGTGGAGGAA CGCTTAGCCC TGCATGTCCT TCAACAACAA 
GGCTTGGTCC CTGAGCATGT GGAGTCTCGG CCTCTGTACA GTCCTCTGCA GCCAGATATT 
GAGCAGGGGA AGCTACAGAT GTGGATTGAC ATATTTCCAA AGGTGCTGGG CCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAAAGCT CGAAGGTTTT TTCTGCGTTG TATTATTTGG 
AACACAAAAG ATGTGATCTT GGATGACCTC AGCCTCACGG GGGAGAAGAT GAGTGACATC 
TATGTGAAAG GCTGGATGGT GGGATTCGAA GAACACAAAC AGAAGACAGA TGTGCACTAC 
CGTTCCCTGG GCGGGGAGGG CAATTTCAAC TGGAGGTTCG TGTTTCCCTT CGACTATCTG 
CCCGCTGAGC AAGTCTGTGC TGTTGCCAAG AAGGATGCTT TCTGGAGACT CGACAAGACA 
GAGAGCAAGA TCCCAGCACG TGTGGTCTTC CAGATTTGGG ACAATGACAA GTTCTCCTTT 
GATGACTTTC TGGGCTCCCT GCAGCTGGAT CTCAACCGAA TGCCCAAGCC AGCCAAGACG 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACCTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAGACAGT GAAAGGATGG TGGCCTTGTG TGACAGAGGA GGGCGCGAAG 
AAGATCCTGG CGGGGAAGCT GGAAATGACC TTGGAGATCG TTGCAGAAAG TGAACATGAG 
GAGCGGCCTG CTGGCCAGGG TCGGGATGAA CCCAACATGA ATCCAAAGCT AGAAGATCCC 
AGGCGCCCTG ACACCTCTTT CCTGTGGTTC ACCTCACCAT ACAAGACCAT GAAGTTCATC 
TTGTGGCGAC GATTCCGGTG TGCCATCATC CTCTTCATCA TCCTCTTCAT CCTCCTGCTC 
TTCCTGGGTG TCTTTGTCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236773.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236835.1 Rattus norvegicus dysferlin (Dysf), transcript variant X19, mRNA.

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CloneID	ORa27414
Clone ID Related Accession (Same CDS sequence)	XM_006236773.3
Accession Version	XM_006236773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTACTCAAGA GTCCAGCCAC TGACCTGGAT GACAACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTATG TGAACCTCTA TGGCAGTCCC CGAGAGTTCA CGGGCTTCCC AGATCCTTAT 
GCGGAGCTCA ACACAGGAAA GGGAGAAGGT GTGGCATATA GGGGCAGGCT TCTGTTGTCC 
CTGGAGACCA AACTGGTGGA GCACAGTGAA CAGAAGGTGG AAGACCTTCC TGCTGATGAC 
ATCTTGAGAG TGGAGAAGTA CCTGCGGAGG CGCAAATATT CCCTCTTTGC TGCCTTCTAC 
TCAGCCACAA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TACGGAAACA AGTTTGACAC CACCTGCTTG CCGTTGGCCT CTACCACCCA ATACAGCCGG 
GCAGTCTTTG ATGGATGCCA CTACTATTAT TTGCCCTGGG GCAACGTGAA GCCTGTGGTA 
GTACTGTCCT CATACTGGGA AGACATCAGC CATCGAGTCG AGACTCAGAA CCAGCTCCTC 
AGGATCGCTG ACCGACTGGA AGCTAACCTG GAGCAAGTCC ACCTGGCTCT GAAGGCACAG 
TGTTCCTCTG AGGATGTGGA CGCCCTGGTG GCTCAGTTGA CAGATGAGCT CCTTGCAGAC 
TGCAGCCAGC CCCTGTGTGA CATCCATGAA ATACCTTCTG CCACCCACCT TGATCAGTAC 
CTGTTCCGCC TCCGCTCCCG TCACCTGAGC CAGATCAAAG AAGCTGCCCT GGCCTTGAAG 
CTTGGCCACA GTGAGCTCTC CACTGCCCTG GAGCAGGCCG AGGACTGGCT TCTGCATCTC 
CGTGCCCTGG CTGAGGAGCC CCAGAACAGC CTGCCAGATA TCATCATCTG GATGCTGCAG 
GGTGACAAGC GTGTGGCCTA CCAACGGGTG CCTGCCCATG AAGTCCTCTT CTCCCGGCGG 
GGCCCCAGCT ACTGCGGCAG GAACTGTGGG AATCTGCAGA CCATCTTTCT GAAATATCCT 
ATGGAAGGCA TGCCTGGGCC CCGGATGCCG GTGCAGATCC GGATCAAGCT CTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA ATTCAATCAG TTTGCAGAGG GGAAGCTCTC TGTCTTTGCT 
GAAACGTATG AGAACCAGAC AAAGCTGGCG CTGGTCGGGA ACTGGGGCAC GACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACAGGC AAGATCAAAC TACCGAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGGCCTG GGCAGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGATGCTG ACGCCGGTCA TCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACTCGG 
CTACCTGGAG GCCAGTGGAT CTATATGAGT GACAACTACA CCGACGTGAA TGGGGAGAAG 
GTGCTTCCAA AGGACGATAT TGAATGCCCG CTGGGTTGGA AGTGGGAAGA TGAAGAATGG 
TCCACAGACC TCAATCGGGC TGTTGATGAA CAAGGCTGGG AGTACAGCAT CACCATACCC 
CCAGACCGAA AGCCCAAACA CTGGGTCCCT GTTGAGAAGA TGTATTACAC TCACAGGCGT 
CGACGCTGGG TCCGACTGCG CAGGAGGGAC CTCAGCCAGA TGGAGGCACT GAAGAAGCAC 
AGGCAGGCAG AGGCTGAGGG CGAGGGCTGG GAATACGCCT CTCTCTTTGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC AGATGCCTTT CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCGGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCATG 
GTGGATGACA AGAGCGAAGA TTCCATGTCT GTGTCTACCC TGAGCTTTGG CGTTAACAGA 
CCCACAATCT CCTGCATCTT TGACTATGGG AACCGCTACC ATCTGCGTTG CTACCTGTAC 
CAGGCCCGGG ACCTGCCCGC CATGGACAAA GACTCCTTCT CTGATCCCTA TGCCATTGTC 
TCCTTCCTGC ATCAAAGCCA GAAGACGGTG GTGGAGAAGA ACACCCTGAA CCCCACCTGG 
GATCAAACCC TCATCTTCTA TGAGATAGAG ATCTTTGGCG AGCCGGCCAG TGTCGCCGAG 
CGGCCACCCA GCATTGTGGT GGAGCTGTAT GACCATGACA CCTATGGCGC TGACGAGTTC 
ATGGGCCGCT GTATTTGTCA GCCAAGCCTG GAACGGATGC CCCGCCTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC TGCCGGAGAG CTGCTGGCAG CGTTCGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCATCA CATCCCTGGC TTTGAGATGC ACGAAACTTC AAGGATTTTG 
GATGAGACGG AGGACACAGA CTTGCCTTAC CCACCACCTC AAAGGGAGGC CAACATCTAC 
ATGGTTCCCC AGAACATCAA GCCAGCTCTC CAGCGTACAG CCATCGAGAT CCTGGCCTGG 
GGCCTTCGGA ACATAAAGAG CTATCAGATG GCCAGCGTGT CTTCCCCCAG CCTCGTGGTG 
GAGTGTGGTG GGCAGACAGT GCAGTCCTGT GTCATCAGAA ACCTTCGGAA GAACCCCAAC 
TTTGACGTCT GTACTCTGTT CATGGAAGTG ATGTTACCCA GAGAGGACCT CTACTGTCCC 
CCTATTGTGG TCAAGGTCAT TGATAATCGC CAGTTTGGCC GCAGGCCAGT TGTGGGTCAG 
TGCACCATTC GCTCCCTGGA GAACTTCCTG TGTGACCCGT ACTCAGCAGA GAGTCCATCC 
CCGCAAGGTG GCCCAGATGA CGTGAGCTTA CTCAGCCCTG GGGAAGACGT GCTCATTGAC 
ATTGATGACA AGGAGCCTCT CATCCCTGTC CAGGAGGAGG AGTTCATTGA CTGGTGGAGC 
AAATTCTTTG CTTCTATCGG GGAGAGGGAA AAGTGTGGTT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGG AGGCCTGTCC 
GACTTTTGTA ATACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGACAC AGAAGATCCA 
TCTGTCATCG GAGAATTTAA GGGTCTCTTC AAAATTTATC CCCTCCCAGA AGATCCAGCC 
ATCCCTATGC CCCCGAGACA ATTCCATCAG CTGGCTGCTC AGGGTCCCCA GGAATGCTTG 
GTCCGGATTT ACATCATCCG AGCATTTGGC TTACAGCCCA AGGACCCCAA TGGCAAGTGC 
GACCCTTACA TCAAGATCTC TATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCTTGTACCC TGGAGCCTGT ATTTGGAAAG ATGTTTGAAC TCACCTGCAC TCTACCTCTG 
GAGAAGGACT TGAAGATCAC ACTCTATGAC TATGACCTGC TCTCCAAGGA TGAGAAGATT 
GGGGAGACAG TCATTGATCT GGAGAATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGC 
CTTCCACAGA CCTACTGTGT CTCTGGACCA AATCAGTGGA GAGATCAACT CCGCCCTTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC AGGGTCAAGG CCCCCGTGTA CCGGACAGAC 
CGAGTGACGT TTCAGGATAA GGAATACACC ATTCAGGAGA TAGAGGCTGG CAGACTCCCA 
AACCCACACC TGGGCCCGGT GGAGGAACGC TTAGCCCTGC ATGTCCTTCA ACAACAAGGC 
TTGGTCCCTG AGCATGTGGA GTCTCGGCCT CTGTACAGTC CTCTGCAGCC AGATATTGAG 
CAGGGGAAGC TACAGATGTG GATTGACATA TTTCCAAAGG TGCTGGGCCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAAAGCTCGA AGGTTTTTTC TGCGTTGTAT TATTTGGAAC 
ACAAAAGATG TGATCTTGGA TGACCTCAGC CTCACGGGGG AGAAGATGAG TGACATCTAT 
GTGAAAGGCT GGATGGTGGG ATTCGAAGAA CACAAACAGA AGACAGATGT GCACTACCGT 
TCCCTGGGCG GGGAGGGCAA TTTCAACTGG AGGTTCGTGT TTCCCTTCGA CTATCTGCCC 
GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG GATGCTTTCT GGAGACTCGA CAAGACAGAG 
AGCAAGATCC CAGCACGTGT GGTCTTCCAG ATTTGGGACA ATGACAAGTT CTCCTTTGAT 
GACTTTCTGG GCTCCCTGCA GCTGGATCTC AACCGAATGC CCAAGCCAGC CAAGACGGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACCTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AGACAGTGAA AGGATGGTGG CCTTGTGTGA CAGAGGAGGG CGCGAAGAAG 
ATCCTGGCGG GGAAGCTGGA AATGACCTTG GAGATCGTTG CAGAAAGTGA ACATGAGGAG 
CGGCCTGCTG GCCAGGGTCG GGATGAACCC AACATGAATC CAAAGCTAGA AGATCCCAGG 
CGCCCTGACA CCTCTTTCCT GTGGTTCACC TCACCATACA AGACCATGAA GTTCATCTTG 
TGGCGACGAT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT CCTGCTCTTC 
CTGGGTGTCT TTGTCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236835.1
CDS	397..6642
Translation	MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEEEPAGVLKSPATDLDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236773.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236773.3

ORF Insert Sequence:

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTACTCAAGA GTCCAGCCAC TGACCTGGAT GACAACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTATG TGAACCTCTA TGGCAGTCCC CGAGAGTTCA CGGGCTTCCC AGATCCTTAT 
GCGGAGCTCA ACACAGGAAA GGGAGAAGGT GTGGCATATA GGGGCAGGCT TCTGTTGTCC 
CTGGAGACCA AACTGGTGGA GCACAGTGAA CAGAAGGTGG AAGACCTTCC TGCTGATGAC 
ATCTTGAGAG TGGAGAAGTA CCTGCGGAGG CGCAAATATT CCCTCTTTGC TGCCTTCTAC 
TCAGCCACAA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TCGAGGTCAG CATTGGGAAC 
TACGGAAACA AGTTTGACAC CACCTGCTTG CCGTTGGCCT CTACCACCCA ATACAGCCGG 
GCAGTCTTTG ATGGATGCCA CTACTATTAT TTGCCCTGGG GCAACGTGAA GCCTGTGGTA 
GTACTGTCCT CATACTGGGA AGACATCAGC CATCGAGTCG AGACTCAGAA CCAGCTCCTC 
AGGATCGCTG ACCGACTGGA AGCTAACCTG GAGCAAGTCC ACCTGGCTCT GAAGGCACAG 
TGTTCCTCTG AGGATGTGGA CGCCCTGGTG GCTCAGTTGA CAGATGAGCT CCTTGCAGAC 
TGCAGCCAGC CCCTGTGTGA CATCCATGAA ATACCTTCTG CCACCCACCT TGATCAGTAC 
CTGTTCCGCC TCCGCTCCCG TCACCTGAGC CAGATCAAAG AAGCTGCCCT GGCCTTGAAG 
CTTGGCCACA GTGAGCTCTC CACTGCCCTG GAGCAGGCCG AGGACTGGCT TCTGCATCTC 
CGTGCCCTGG CTGAGGAGCC CCAGAACAGC CTGCCAGATA TCATCATCTG GATGCTGCAG 
GGTGACAAGC GTGTGGCCTA CCAACGGGTG CCTGCCCATG AAGTCCTCTT CTCCCGGCGG 
GGCCCCAGCT ACTGCGGCAG GAACTGTGGG AATCTGCAGA CCATCTTTCT GAAATATCCT 
ATGGAAGGCA TGCCTGGGCC CCGGATGCCG GTGCAGATCC GGATCAAGCT CTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA ATTCAATCAG TTTGCAGAGG GGAAGCTCTC TGTCTTTGCT 
GAAACGTATG AGAACCAGAC AAAGCTGGCG CTGGTCGGGA ACTGGGGCAC GACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACAGGC AAGATCAAAC TACCGAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGGCCTG GGCAGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTGCTC 
CATGATGCTG ACGCCGGTCA TCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACTCGG 
CTACCTGGAG GCCAGTGGAT CTATATGAGT GACAACTACA CCGACGTGAA TGGGGAGAAG 
GTGCTTCCAA AGGACGATAT TGAATGCCCG CTGGGTTGGA AGTGGGAAGA TGAAGAATGG 
TCCACAGACC TCAATCGGGC TGTTGATGAA CAAGGCTGGG AGTACAGCAT CACCATACCC 
CCAGACCGAA AGCCCAAACA CTGGGTCCCT GTTGAGAAGA TGTATTACAC TCACAGGCGT 
CGACGCTGGG TCCGACTGCG CAGGAGGGAC CTCAGCCAGA TGGAGGCACT GAAGAAGCAC 
AGGCAGGCAG AGGCTGAGGG CGAGGGCTGG GAATACGCCT CTCTCTTTGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC AGATGCCTTT CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCGGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCATG 
GTGGATGACA AGAGCGAAGA TTCCATGTCT GTGTCTACCC TGAGCTTTGG CGTTAACAGA 
CCCACAATCT CCTGCATCTT TGACTATGGG AACCGCTACC ATCTGCGTTG CTACCTGTAC 
CAGGCCCGGG ACCTGCCCGC CATGGACAAA GACTCCTTCT CTGATCCCTA TGCCATTGTC 
TCCTTCCTGC ATCAAAGCCA GAAGACGGTG GTGGAGAAGA ACACCCTGAA CCCCACCTGG 
GATCAAACCC TCATCTTCTA TGAGATAGAG ATCTTTGGCG AGCCGGCCAG TGTCGCCGAG 
CGGCCACCCA GCATTGTGGT GGAGCTGTAT GACCATGACA CCTATGGCGC TGACGAGTTC 
ATGGGCCGCT GTATTTGTCA GCCAAGCCTG GAACGGATGC CCCGCCTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC TGCCGGAGAG CTGCTGGCAG CGTTCGAGCT TATCCAGAGA 
GAGAAGCCAG CCATCCATCA CATCCCTGGC TTTGAGATGC ACGAAACTTC AAGGATTTTG 
GATGAGACGG AGGACACAGA CTTGCCTTAC CCACCACCTC AAAGGGAGGC CAACATCTAC 
ATGGTTCCCC AGAACATCAA GCCAGCTCTC CAGCGTACAG CCATCGAGAT CCTGGCCTGG 
GGCCTTCGGA ACATAAAGAG CTATCAGATG GCCAGCGTGT CTTCCCCCAG CCTCGTGGTG 
GAGTGTGGTG GGCAGACAGT GCAGTCCTGT GTCATCAGAA ACCTTCGGAA GAACCCCAAC 
TTTGACGTCT GTACTCTGTT CATGGAAGTG ATGTTACCCA GAGAGGACCT CTACTGTCCC 
CCTATTGTGG TCAAGGTCAT TGATAATCGC CAGTTTGGCC GCAGGCCAGT TGTGGGTCAG 
TGCACCATTC GCTCCCTGGA GAACTTCCTG TGTGACCCGT ACTCAGCAGA GAGTCCATCC 
CCGCAAGGTG GCCCAGATGA CGTGAGCTTA CTCAGCCCTG GGGAAGACGT GCTCATTGAC 
ATTGATGACA AGGAGCCTCT CATCCCTGTC CAGGAGGAGG AGTTCATTGA CTGGTGGAGC 
AAATTCTTTG CTTCTATCGG GGAGAGGGAA AAGTGTGGTT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGG AGGCCTGTCC 
GACTTTTGTA ATACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGACAC AGAAGATCCA 
TCTGTCATCG GAGAATTTAA GGGTCTCTTC AAAATTTATC CCCTCCCAGA AGATCCAGCC 
ATCCCTATGC CCCCGAGACA ATTCCATCAG CTGGCTGCTC AGGGTCCCCA GGAATGCTTG 
GTCCGGATTT ACATCATCCG AGCATTTGGC TTACAGCCCA AGGACCCCAA TGGCAAGTGC 
GACCCTTACA TCAAGATCTC TATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCTTGTACCC TGGAGCCTGT ATTTGGAAAG ATGTTTGAAC TCACCTGCAC TCTACCTCTG 
GAGAAGGACT TGAAGATCAC ACTCTATGAC TATGACCTGC TCTCCAAGGA TGAGAAGATT 
GGGGAGACAG TCATTGATCT GGAGAATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGC 
CTTCCACAGA CCTACTGTGT CTCTGGACCA AATCAGTGGA GAGATCAACT CCGCCCTTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAC AGGGTCAAGG CCCCCGTGTA CCGGACAGAC 
CGAGTGACGT TTCAGGATAA GGAATACACC ATTCAGGAGA TAGAGGCTGG CAGACTCCCA 
AACCCACACC TGGGCCCGGT GGAGGAACGC TTAGCCCTGC ATGTCCTTCA ACAACAAGGC 
TTGGTCCCTG AGCATGTGGA GTCTCGGCCT CTGTACAGTC CTCTGCAGCC AGATATTGAG 
CAGGGGAAGC TACAGATGTG GATTGACATA TTTCCAAAGG TGCTGGGCCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAAAGCTCGA AGGTTTTTTC TGCGTTGTAT TATTTGGAAC 
ACAAAAGATG TGATCTTGGA TGACCTCAGC CTCACGGGGG AGAAGATGAG TGACATCTAT 
GTGAAAGGCT GGATGGTGGG ATTCGAAGAA CACAAACAGA AGACAGATGT GCACTACCGT 
TCCCTGGGCG GGGAGGGCAA TTTCAACTGG AGGTTCGTGT TTCCCTTCGA CTATCTGCCC 
GCTGAGCAAG TCTGTGCTGT TGCCAAGAAG GATGCTTTCT GGAGACTCGA CAAGACAGAG 
AGCAAGATCC CAGCACGTGT GGTCTTCCAG ATTTGGGACA ATGACAAGTT CTCCTTTGAT 
GACTTTCTGG GCTCCCTGCA GCTGGATCTC AACCGAATGC CCAAGCCAGC CAAGACGGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACCTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AGACAGTGAA AGGATGGTGG CCTTGTGTGA CAGAGGAGGG CGCGAAGAAG 
ATCCTGGCGG GGAAGCTGGA AATGACCTTG GAGATCGTTG CAGAAAGTGA ACATGAGGAG 
CGGCCTGCTG GCCAGGGTCG GGATGAACCC AACATGAATC CAAAGCTAGA AGATCCCAGG 
CGCCCTGACA CCTCTTTCCT GTGGTTCACC TCACCATACA AGACCATGAA GTTCATCTTG 
TGGCGACGAT TCCGGTGTGC CATCATCCTC TTCATCATCC TCTTCATCCT CCTGCTCTTC 
CTGGGTGTCT TTGTCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236774.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236836.1 Rattus norvegicus dysferlin (Dysf), transcript variant X20, mRNA.

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CloneID	ORa27415
Clone ID Related Accession (Same CDS sequence)	XM_006236774.3
Accession Version	XM_006236774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGT GGATGACAAC 
CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG 
TTCACGGGCT TCCCAGATCC TTATGCGGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCA 
TATAGGGGCA GGCTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAGCACAG TGAACAGAAG 
GTGGAAGACC TTCCTGCTGA TGACATCTTG AGAGTGGAGA AGTACCTGCG GAGGCGCAAA 
TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATTGG GAACTACGGA AACAAGTTTG ACACCACCTG CTTGCCGTTG 
GCCTCTACCA CCCAATACAG CCGGGCAGTC TTTGATGGAT GCCACTACTA TTATTTGCCC 
TGGGGCAACG TGAAGCCTGT GGTAGTACTG TCCTCATACT GGGAAGACAT CAGCCATCGA 
GTCGAGACTC AGAACCAGCT CCTCAGGATC GCTGACCGAC TGGAAGCTAA CCTGGAGCAA 
GTCCACCTGG CTCTGAAGGC ACAGTGTTCC TCTGAGGATG TGGACGCCCT GGTGGCTCAG 
TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA TGAAATACCT 
TCTGCCACCC ACCTTGATCA GTACCTGTTC CGCCTCCGCT CCCGTCACCT GAGCCAGATC 
AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC CACAGTGAGC TCTCCACTGC CCTGGAGCAG 
GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA 
GATATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAACG GGTGCCTGCC 
CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAATCTG 
CAGACCATCT TTCTGAAATA TCCTATGGAA GGCATGCCTG GGCCCCGGAT GCCGGTGCAG 
ATCCGGATCA AGCTCTGGTT TGGGCTCTCA GTGGATGAGA AGGAATTCAA TCAGTTTGCA 
GAGGGGAAGC TCTCTGTCTT TGCTGAAACG TATGAGAACC AGACAAAGCT GGCGCTGGTC 
GGGAACTGGG GCACGACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC 
AAACTACCGA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT GCTCCATGAT GCTGACGCCG GTCATCTGAG CTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC TCGGCTACCT GGAGGCCAGT GGATCTATAT GAGTGACAAC 
TACACCGACG TGAATGGGGA GAAGGTGCTT CCAAAGGACG ATATTGAATG CCCGCTGGGT 
TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCCA AACACTGGGT CCCTGTTGAG 
AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CACTGAAGAA GCACAGGCAG GCAGAGGCTG AGGGCGAGGG CTGGGAATAC 
GCCTCTCTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACGG GGCCTGCAGC GGTGTTTGCC 
CTTGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGATTCCAT GTCTGTGTCT 
ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTGC GTTGCTACCT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAAGACTCC 
TTCTCTGATC CCTATGCCAT TGTCTCCTTC CTGCATCAAA GCCAGAAGAC GGTGGTGGAG 
AAGAACACCC TGAACCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT AGAGATCTTT 
GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA CCCAGCATTG TGGTGGAGCT GTATGACCAT 
GACACCTATG GCGCTGACGA GTTCATGGGC CGCTGTATTT GTCAGCCAAG CCTGGAACGG 
ATGCCCCGCC TGGCCTGGTT CCCACTGACC AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG 
GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG 
ATGCACGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGACTTGCC TTACCCACCA 
CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCAGC TCTCCAGCGT 
ACAGCCATCG AGATCCTGGC CTGGGGCCTT CGGAACATAA AGAGCTATCA GATGGCCAGC 
GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC 
AGAAACCTTC GGAAGAACCC CAACTTTGAC GTCTGTACTC TGTTCATGGA AGTGATGTTA 
CCCAGAGAGG ACCTCTACTG TCCCCCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT 
GGCCGCAGGC CAGTTGTGGG TCAGTGCACC ATTCGCTCCC TGGAGAACTT CCTGTGTGAC 
CCGTACTCAG CAGAGAGTCC ATCCCCGCAA GGTGGCCCAG ATGACGTGAG CTTACTCAGC 
CCTGGGGAAG ACGTGCTCAT TGACATTGAT GACAAGGAGC CTCTCATCCC TGTCCAGGAG 
GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTA TCGGGGAGAG GGAAAAGTGT 
GGTTCCTACC TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGGAGGCCT GTCCGACTTT TGTAATACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG ACACAGAAGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT 
TATCCCCTCC CAGAAGATCC AGCCATCCCT ATGCCCCCGA GACAATTCCA TCAGCTGGCT 
GCTCAGGGTC CCCAGGAATG CTTGGTCCGG ATTTACATCA TCCGAGCATT TGGCTTACAG 
CCCAAGGACC CCAATGGCAA GTGCGACCCT TACATCAAGA TCTCTATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCTTGT ACCCTGGAGC CTGTATTTGG AAAGATGTTT 
GAACTCACCT GCACTCTACC TCTGGAGAAG GACTTGAAGA TCACACTCTA TGACTATGAC 
CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACAGTCATTG ATCTGGAGAA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGCCTTCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG 
TGGAGAGATC AACTCCGCCC TTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGAATA CACCATTCAG 
GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CGGTGGAGGA ACGCTTAGCC 
CTGCATGTCC TTCAACAACA AGGCTTGGTC CCTGAGCATG TGGAGTCTCG GCCTCTGTAC 
AGTCCTCTGC AGCCAGATAT TGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA 
AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TCGAAGGTTT 
TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGCCTCACG 
GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAA 
CAGAAGACAG ATGTGCACTA CCGTTCCCTG GGCGGGGAGG GCAATTTCAA CTGGAGGTTC 
GTGTTTCCCT TCGACTATCT GCCCGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCT 
TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATTTGG 
GACAATGACA AGTTCTCCTT TGATGACTTT CTGGGCTCCC TGCAGCTGGA TCTCAACCGA 
ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT 
GTGACAGAGG AGGGCGCGAA GAAGATCCTG GCGGGGAAGC TGGAAATGAC CTTGGAGATC 
GTTGCAGAAA GTGAACATGA GGAGCGGCCT GCTGGCCAGG GTCGGGATGA ACCCAACATG 
AATCCAAAGC TAGAAGATCC CAGGCGCCCT GACACCTCTT TCCTGTGGTT CACCTCACCA 
TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGATTCCGGT GTGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236836.1
CDS	702..6911
Translation	MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236774.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236774.3

ORF Insert Sequence:

ATGCTCTGCT GCCTGCTGGC AAGGGCCAGC AACCTCCCCA ATGTGAAGAA GGACCGGCGC 
AGTGACCCTG TTGCCAGCCT GATTTTTCGA GGGGTAAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAAGG TATTCCTCTA 
GACCAGAGCT CAGAACTTCT CGTGGTGGTC AAGGACCATG AGACGATGGG GAGAAACAGG 
TTCCTGGGAG AAGCCAAGAT CCCACTCCAG GAGGTCCTTG CCACCCCCAG CCTATCTGCC 
AGCTTCAACG CACCTCTGCT GGACTCCAAG CAGCAACCCA CAGGGGCCTC TCTGGTCCTG 
CAGGTATCCT ACACACCGCC CCCAGGAGCT GCGCCCTTGT TCCCACCACC CGCTTCCCTG 
GCACCGTCCC CAACACTGCC TGACATGGAC CTGGTGGCTG ACACAGGAGG AGAGGAAGAC 
ACGGAAGACC AGGGCCTGAC TGGAGATGAA GCTGAGCTCT TCCCAGATCC GAGTGTTGCT 
GTGGGCCCAG GGGGACCAAC GACGCCCAAG AAGCCACCTT CCCATCCTCC TCCCCACTAT 
CCAGGGGGGA AAAGGAAAAG AAGCTCTGCT CCACCTAGAA AGCTGCTGTC AGACAAGCCA 
CAGGACTTCC AGATCAGAGT CCAGGTGATT GAGGGGCGTC AGCTGCCTGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC AGCTGCTGGG CAGACCAAGC GGACTCGAAT CCAGAAGGGA 
AATAGCCCAC TTTTCAATGA GACTCTTTTC TTCAACGTAT TTGACTCTCC CTTGGAACTG 
TTTGATGAGC CCATCTTCAT CACGGTGGTA GACTCACGCT CCCTCAGGAC AGATGCCCTC 
CTTGGCGAGT TCCGGATGGA TGTGGGCACC GTGTACAGAG AGCCCCGACA TGCTTATCTC 
CGGAAGTGGC TGCTGCTCTC CGATCCCGAT GACTTCTCTG CAGGGGCCAG AGGCTACCTC 
AAAGCCAGCC TGTGTGTGCT GGGGCCTGGG GACGAAGCTC CCCTAGACAA GAAAGACCCT 
TCTGAAGACA AAGAGGACAT TGAAGGCAAC CTGCTTAGGC CCACTGGTGT GGCCCTTCGT 
GGTGCCCATT TTTGCCTGAA GCTCTTCCGG GCTGAGGACT TACCACAGAT GGACGATGCT 
GTGATGGACA ACGTCAAGCA AATCTTTGGC TTTGATAGCA ACAAGAAGAA CCTGGTGGAC 
CCCTTTGTGG AAGTCAGCTT TGCTGGGAAA ATGCTTTGCA GCAAGATCCT GGAGAAGACT 
GCTAACCCTC AGTGGAACCA GAACATCACA TTGCCTGCGA TGTTTCCCTC TATGTGTGAA 
AAAATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACTC ACAATGACAC TGTGGCTACC 
ACCTACTTGG GTATGTCGAA AATCTCTGCC ACTGGAGGAG AAATAGAAGT GGATGACAAC 
CTGGGCTTCC TCCCCACTTT TGGGCCCTGC TATGTGAACC TCTATGGCAG TCCCCGAGAG 
TTCACGGGCT TCCCAGATCC TTATGCGGAG CTCAACACAG GAAAGGGAGA AGGTGTGGCA 
TATAGGGGCA GGCTTCTGTT GTCCCTGGAG ACCAAACTGG TGGAGCACAG TGAACAGAAG 
GTGGAAGACC TTCCTGCTGA TGACATCTTG AGAGTGGAGA AGTACCTGCG GAGGCGCAAA 
TATTCCCTCT TTGCTGCCTT CTACTCAGCC ACAATGCTGC AGGATGTGGA TGATGCCATC 
CAGTTCGAGG TCAGCATTGG GAACTACGGA AACAAGTTTG ACACCACCTG CTTGCCGTTG 
GCCTCTACCA CCCAATACAG CCGGGCAGTC TTTGATGGAT GCCACTACTA TTATTTGCCC 
TGGGGCAACG TGAAGCCTGT GGTAGTACTG TCCTCATACT GGGAAGACAT CAGCCATCGA 
GTCGAGACTC AGAACCAGCT CCTCAGGATC GCTGACCGAC TGGAAGCTAA CCTGGAGCAA 
GTCCACCTGG CTCTGAAGGC ACAGTGTTCC TCTGAGGATG TGGACGCCCT GGTGGCTCAG 
TTGACAGATG AGCTCCTTGC AGACTGCAGC CAGCCCCTGT GTGACATCCA TGAAATACCT 
TCTGCCACCC ACCTTGATCA GTACCTGTTC CGCCTCCGCT CCCGTCACCT GAGCCAGATC 
AAAGAAGCTG CCCTGGCCTT GAAGCTTGGC CACAGTGAGC TCTCCACTGC CCTGGAGCAG 
GCCGAGGACT GGCTTCTGCA TCTCCGTGCC CTGGCTGAGG AGCCCCAGAA CAGCCTGCCA 
GATATCATCA TCTGGATGCT GCAGGGTGAC AAGCGTGTGG CCTACCAACG GGTGCCTGCC 
CATGAAGTCC TCTTCTCCCG GCGGGGCCCC AGCTACTGCG GCAGGAACTG TGGGAATCTG 
CAGACCATCT TTCTGAAATA TCCTATGGAA GGCATGCCTG GGCCCCGGAT GCCGGTGCAG 
ATCCGGATCA AGCTCTGGTT TGGGCTCTCA GTGGATGAGA AGGAATTCAA TCAGTTTGCA 
GAGGGGAAGC TCTCTGTCTT TGCTGAAACG TATGAGAACC AGACAAAGCT GGCGCTGGTC 
GGGAACTGGG GCACGACGGG CCTCACCTAC CCCAAGTTTT CTGACGTCAC AGGCAAGATC 
AAACTACCGA AGGATAGCTT CCGCCCCTCA GCTGGCTGGG CCTGGGCAGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT GCTCCATGAT GCTGACGCCG GTCATCTGAG CTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC TCGGCTACCT GGAGGCCAGT GGATCTATAT GAGTGACAAC 
TACACCGACG TGAATGGGGA GAAGGTGCTT CCAAAGGACG ATATTGAATG CCCGCTGGGT 
TGGAAGTGGG AAGATGAAGA ATGGTCCACA GACCTCAATC GGGCTGTTGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT ACCCCCAGAC CGAAAGCCCA AACACTGGGT CCCTGTTGAG 
AAGATGTATT ACACTCACAG GCGTCGACGC TGGGTCCGAC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CACTGAAGAA GCACAGGCAG GCAGAGGCTG AGGGCGAGGG CTGGGAATAC 
GCCTCTCTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGATGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCACTG GAGAAGACGG GGCCTGCAGC GGTGTTTGCC 
CTTGAGGGGG CCCTGGGCGG CATGGTGGAT GACAAGAGCG AAGATTCCAT GTCTGTGTCT 
ACCCTGAGCT TTGGCGTTAA CAGACCCACA ATCTCCTGCA TCTTTGACTA TGGGAACCGC 
TACCATCTGC GTTGCTACCT GTACCAGGCC CGGGACCTGC CCGCCATGGA CAAAGACTCC 
TTCTCTGATC CCTATGCCAT TGTCTCCTTC CTGCATCAAA GCCAGAAGAC GGTGGTGGAG 
AAGAACACCC TGAACCCCAC CTGGGATCAA ACCCTCATCT TCTATGAGAT AGAGATCTTT 
GGCGAGCCGG CCAGTGTCGC CGAGCGGCCA CCCAGCATTG TGGTGGAGCT GTATGACCAT 
GACACCTATG GCGCTGACGA GTTCATGGGC CGCTGTATTT GTCAGCCAAG CCTGGAACGG 
ATGCCCCGCC TGGCCTGGTT CCCACTGACC AGGGGCAGCC AGCCTGCCGG AGAGCTGCTG 
GCAGCGTTCG AGCTTATCCA GAGAGAGAAG CCAGCCATCC ATCACATCCC TGGCTTTGAG 
ATGCACGAAA CTTCAAGGAT TTTGGATGAG ACGGAGGACA CAGACTTGCC TTACCCACCA 
CCTCAAAGGG AGGCCAACAT CTACATGGTT CCCCAGAACA TCAAGCCAGC TCTCCAGCGT 
ACAGCCATCG AGATCCTGGC CTGGGGCCTT CGGAACATAA AGAGCTATCA GATGGCCAGC 
GTGTCTTCCC CCAGCCTCGT GGTGGAGTGT GGTGGGCAGA CAGTGCAGTC CTGTGTCATC 
AGAAACCTTC GGAAGAACCC CAACTTTGAC GTCTGTACTC TGTTCATGGA AGTGATGTTA 
CCCAGAGAGG ACCTCTACTG TCCCCCTATT GTGGTCAAGG TCATTGATAA TCGCCAGTTT 
GGCCGCAGGC CAGTTGTGGG TCAGTGCACC ATTCGCTCCC TGGAGAACTT CCTGTGTGAC 
CCGTACTCAG CAGAGAGTCC ATCCCCGCAA GGTGGCCCAG ATGACGTGAG CTTACTCAGC 
CCTGGGGAAG ACGTGCTCAT TGACATTGAT GACAAGGAGC CTCTCATCCC TGTCCAGGAG 
GAGGAGTTCA TTGACTGGTG GAGCAAATTC TTTGCTTCTA TCGGGGAGAG GGAAAAGTGT 
GGTTCCTACC TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGGAGGCCT GTCCGACTTT TGTAATACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG ACACAGAAGA TCCATCTGTC ATCGGAGAAT TTAAGGGTCT CTTCAAAATT 
TATCCCCTCC CAGAAGATCC AGCCATCCCT ATGCCCCCGA GACAATTCCA TCAGCTGGCT 
GCTCAGGGTC CCCAGGAATG CTTGGTCCGG ATTTACATCA TCCGAGCATT TGGCTTACAG 
CCCAAGGACC CCAATGGCAA GTGCGACCCT TACATCAAGA TCTCTATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCTTGT ACCCTGGAGC CTGTATTTGG AAAGATGTTT 
GAACTCACCT GCACTCTACC TCTGGAGAAG GACTTGAAGA TCACACTCTA TGACTATGAC 
CTGCTCTCCA AGGATGAGAA GATTGGGGAG ACAGTCATTG ATCTGGAGAA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGCCTTCCA CAGACCTACT GTGTCTCTGG ACCAAATCAG 
TGGAGAGATC AACTCCGCCC TTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC AGACCGAGTG ACGTTTCAGG ATAAGGAATA CACCATTCAG 
GAGATAGAGG CTGGCAGACT CCCAAACCCA CACCTGGGCC CGGTGGAGGA ACGCTTAGCC 
CTGCATGTCC TTCAACAACA AGGCTTGGTC CCTGAGCATG TGGAGTCTCG GCCTCTGTAC 
AGTCCTCTGC AGCCAGATAT TGAGCAGGGG AAGCTACAGA TGTGGATTGA CATATTTCCA 
AAGGTGCTGG GCCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAAAGC TCGAAGGTTT 
TTTCTGCGTT GTATTATTTG GAACACAAAA GATGTGATCT TGGATGACCT CAGCCTCACG 
GGGGAGAAGA TGAGTGACAT CTATGTGAAA GGCTGGATGG TGGGATTCGA AGAACACAAA 
CAGAAGACAG ATGTGCACTA CCGTTCCCTG GGCGGGGAGG GCAATTTCAA CTGGAGGTTC 
GTGTTTCCCT TCGACTATCT GCCCGCTGAG CAAGTCTGTG CTGTTGCCAA GAAGGATGCT 
TTCTGGAGAC TCGACAAGAC AGAGAGCAAG ATCCCAGCAC GTGTGGTCTT CCAGATTTGG 
GACAATGACA AGTTCTCCTT TGATGACTTT CTGGGCTCCC TGCAGCTGGA TCTCAACCGA 
ATGCCCAAGC CAGCCAAGAC GGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACCTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAGACAG TGAAAGGATG GTGGCCTTGT 
GTGACAGAGG AGGGCGCGAA GAAGATCCTG GCGGGGAAGC TGGAAATGAC CTTGGAGATC 
GTTGCAGAAA GTGAACATGA GGAGCGGCCT GCTGGCCAGG GTCGGGATGA ACCCAACATG 
AATCCAAAGC TAGAAGATCC CAGGCGCCCT GACACCTCTT TCCTGTGGTT CACCTCACCA 
TACAAGACCA TGAAGTTCAT CTTGTGGCGA CGATTCCGGT GTGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTCCTGCT CTTCCTGGGT GTCTTTGTCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dysf ( XM_006236775.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236837.1 Rattus norvegicus dysferlin (Dysf), transcript variant X21, mRNA.

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CloneID	ORa27416
Clone ID Related Accession (Same CDS sequence)	XM_006236775.2
Accession Version	XM_006236775.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dysferlin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006236775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGTGGA TGACAACCTG 
GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC 
ACGGGCTTCC CAGATCCTTA TGCGGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCATAT 
AGGGGCAGGC TTCTGTTGTC CCTGGAGACC AAACTGGTGG AGCACAGTGA ACAGAAGGTG 
GAAGACCTTC CTGCTGATGA CATCTTGAGA GTGGAGAAGT ACCTGCGGAG GCGCAAATAT 
TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA TGCCATCCAG 
TTCGAGGTCA GCATTGGGAA CTACGGAAAC AAGTTTGACA CCACCTGCTT GCCGTTGGCC 
TCTACCACCC AATACAGCCG GGCAGTCTTT GATGGATGCC ACTACTATTA TTTGCCCTGG 
GGCAACGTGA AGCCTGTGGT AGTACTGTCC TCATACTGGG AAGACATCAG CCATCGAGTC 
GAGACTCAGA ACCAGCTCCT CAGGATCGCT GACCGACTGG AAGCTAACCT GGAGCAAGTC 
CACCTGGCTC TGAAGGCACA GTGTTCCTCT GAGGATGTGG ACGCCCTGGT GGCTCAGTTG 
ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCATGA AATACCTTCT 
GCCACCCACC TTGATCAGTA CCTGTTCCGC CTCCGCTCCC GTCACCTGAG CCAGATCAAA 
GAAGCTGCCC TGGCCTTGAA GCTTGGCCAC AGTGAGCTCT CCACTGCCCT GGAGCAGGCC 
GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCTGAGGAGC CCCAGAACAG CCTGCCAGAT 
ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAACGGGT GCCTGCCCAT 
GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAATCTGCAG 
ACCATCTTTC TGAAATATCC TATGGAAGGC ATGCCTGGGC CCCGGATGCC GGTGCAGATC 
CGGATCAAGC TCTGGTTTGG GCTCTCAGTG GATGAGAAGG AATTCAATCA GTTTGCAGAG 
GGGAAGCTCT CTGTCTTTGC TGAAACGTAT GAGAACCAGA CAAAGCTGGC GCTGGTCGGG 
AACTGGGGCA CGACGGGCCT CACCTACCCC AAGTTTTCTG ACGTCACAGG CAAGATCAAA 
CTACCGAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACCCTGCT CCATGATGCT GACGCCGGTC ATCTGAGCTT TGTGGAGGAG 
GTGTTTGAGA ACCAGACTCG GCTACCTGGA GGCCAGTGGA TCTATATGAG TGACAACTAC 
ACCGACGTGA ATGGGGAGAA GGTGCTTCCA AAGGACGATA TTGAATGCCC GCTGGGTTGG 
AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCCAAAC ACTGGGTCCC TGTTGAGAAG 
ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCAC TGAAGAAGCA CAGGCAGGCA GAGGCTGAGG GCGAGGGCTG GGAATACGCC 
TCTCTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT TCGCCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCACTGGAG AAGACGGGGC CTGCAGCGGT GTTTGCCCTT 
GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ATTCCATGTC TGTGTCTACC 
CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC 
CATCTGCGTT GCTACCTGTA CCAGGCCCGG GACCTGCCCG CCATGGACAA AGACTCCTTC 
TCTGATCCCT ATGCCATTGT CTCCTTCCTG CATCAAAGCC AGAAGACGGT GGTGGAGAAG 
AACACCCTGA ACCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATAGA GATCTTTGGC 
GAGCCGGCCA GTGTCGCCGA GCGGCCACCC AGCATTGTGG TGGAGCTGTA TGACCATGAC 
ACCTATGGCG CTGACGAGTT CATGGGCCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG 
CCCCGCCTGG CCTGGTTCCC ACTGACCAGG GGCAGCCAGC CTGCCGGAGA GCTGCTGGCA 
GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG 
CACGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ACTTGCCTTA CCCACCACCT 
CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCAGCTCT CCAGCGTACA 
GCCATCGAGA TCCTGGCCTG GGGCCTTCGG AACATAAAGA GCTATCAGAT GGCCAGCGTG 
TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA 
AACCTTCGGA AGAACCCCAA CTTTGACGTC TGTACTCTGT TCATGGAAGT GATGTTACCC 
AGAGAGGACC TCTACTGTCC CCCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC 
CGCAGGCCAG TTGTGGGTCA GTGCACCATT CGCTCCCTGG AGAACTTCCT GTGTGACCCG 
TACTCAGCAG AGAGTCCATC CCCGCAAGGT GGCCCAGATG ACGTGAGCTT ACTCAGCCCT 
GGGGAAGACG TGCTCATTGA CATTGATGAC AAGGAGCCTC TCATCCCTGT CCAGGAGGAG 
GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTATCG GGGAGAGGGA AAAGTGTGGT 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG GAGGCCTGTC CGACTTTTGT AATACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGACA CAGAAGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT 
CCCCTCCCAG AAGATCCAGC CATCCCTATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT 
CAGGGTCCCC AGGAATGCTT GGTCCGGATT TACATCATCC GAGCATTTGG CTTACAGCCC 
AAGGACCCCA ATGGCAAGTG CGACCCTTAC ATCAAGATCT CTATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCTTGTACC CTGGAGCCTG TATTTGGAAA GATGTTTGAA 
CTCACCTGCA CTCTACCTCT GGAGAAGGAC TTGAAGATCA CACTCTATGA CTATGACCTG 
CTCTCCAAGG ATGAGAAGAT TGGGGAGACA GTCATTGATC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG CCTTCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG 
AGAGATCAAC TCCGCCCTTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGAATACAC CATTCAGGAG 
ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCGG TGGAGGAACG CTTAGCCCTG 
CATGTCCTTC AACAACAAGG CTTGGTCCCT GAGCATGTGG AGTCTCGGCC TCTGTACAGT 
CCTCTGCAGC CAGATATTGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG 
GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTCG AAGGTTTTTT 
CTGCGTTGTA TTATTTGGAA CACAAAAGAT GTGATCTTGG ATGACCTCAG CCTCACGGGG 
GAGAAGATGA GTGACATCTA TGTGAAAGGC TGGATGGTGG GATTCGAAGA ACACAAACAG 
AAGACAGATG TGCACTACCG TTCCCTGGGC GGGGAGGGCA ATTTCAACTG GAGGTTCGTG 
TTTCCCTTCG ACTATCTGCC CGCTGAGCAA GTCTGTGCTG TTGCCAAGAA GGATGCTTTC 
TGGAGACTCG ACAAGACAGA GAGCAAGATC CCAGCACGTG TGGTCTTCCA GATTTGGGAC 
AATGACAAGT TCTCCTTTGA TGACTTTCTG GGCTCCCTGC AGCTGGATCT CAACCGAATG 
CCCAAGCCAG CCAAGACGGC TGAGAAGTGC TCCTTGGACC AGCTGGATGA CACCTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAGACAGTGA AAGGATGGTG GCCTTGTGTG 
ACAGAGGAGG GCGCGAAGAA GATCCTGGCG GGGAAGCTGG AAATGACCTT GGAGATCGTT 
GCAGAAAGTG AACATGAGGA GCGGCCTGCT GGCCAGGGTC GGGATGAACC CAACATGAAT 
CCAAAGCTAG AAGATCCCAG GCGCCCTGAC ACCTCTTTCC TGTGGTTCAC CTCACCATAC 
AAGACCATGA AGTTCATCTT GTGGCGACGA TTCCGGTGTG CCATCATCCT CTTCATCATC 
CTCTTCATCC TCCTGCTCTT CCTGGGTGTC TTTGTCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT TAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236837.1
CDS	395..6601
Translation	MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDSKQQPTGASLVLQVSYTPPPGAAPLFPPPASLAPSPTLPDMDLVADTGGEEDTEDQGLTGDEAELFPDPSVAVGPGGPTTPKKPPSHPPPHYPGGKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVETQNQLLRIADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLFRLRSRHLSQIKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSYCGRNCGNLQTIFLKYPMEGMPGPRMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEALKKHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASVAERPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNIKSYQMASVSSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDFCNTFKLYRGKTQEDTEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVTFQDKEYTIQEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGAKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR

Target ORF information:

RefSeq Version	XM_006236775.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dysferlin (Dysf), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236775.2

ORF Insert Sequence:

ATGCTGCGAG TCTTCATTCT CTTTGCGGAG AATGTCCACA CCCCGGACTC CGACATCAGC 
GATGCTTACT GCTCCGCGGT GTTTGCAGGG GTAAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAAGGTAT TCCTCTAGAC 
CAGAGCTCAG AACTTCTCGT GGTGGTCAAG GACCATGAGA CGATGGGGAG AAACAGGTTC 
CTGGGAGAAG CCAAGATCCC ACTCCAGGAG GTCCTTGCCA CCCCCAGCCT ATCTGCCAGC 
TTCAACGCAC CTCTGCTGGA CTCCAAGCAG CAACCCACAG GGGCCTCTCT GGTCCTGCAG 
GTATCCTACA CACCGCCCCC AGGAGCTGCG CCCTTGTTCC CACCACCCGC TTCCCTGGCA 
CCGTCCCCAA CACTGCCTGA CATGGACCTG GTGGCTGACA CAGGAGGAGA GGAAGACACG 
GAAGACCAGG GCCTGACTGG AGATGAAGCT GAGCTCTTCC CAGATCCGAG TGTTGCTGTG 
GGCCCAGGGG GACCAACGAC GCCCAAGAAG CCACCTTCCC ATCCTCCTCC CCACTATCCA 
GGGGGGAAAA GGAAAAGAAG CTCTGCTCCA CCTAGAAAGC TGCTGTCAGA CAAGCCACAG 
GACTTCCAGA TCAGAGTCCA GGTGATTGAG GGGCGTCAGC TGCCTGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACAGC TGCTGGGCAG ACCAAGCGGA CTCGAATCCA GAAGGGAAAT 
AGCCCACTTT TCAATGAGAC TCTTTTCTTC AACGTATTTG ACTCTCCCTT GGAACTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCACGCTCCC TCAGGACAGA TGCCCTCCTT 
GGCGAGTTCC GGATGGATGT GGGCACCGTG TACAGAGAGC CCCGACATGC TTATCTCCGG 
AAGTGGCTGC TGCTCTCCGA TCCCGATGAC TTCTCTGCAG GGGCCAGAGG CTACCTCAAA 
GCCAGCCTGT GTGTGCTGGG GCCTGGGGAC GAAGCTCCCC TAGACAAGAA AGACCCTTCT 
GAAGACAAAG AGGACATTGA AGGCAACCTG CTTAGGCCCA CTGGTGTGGC CCTTCGTGGT 
GCCCATTTTT GCCTGAAGCT CTTCCGGGCT GAGGACTTAC CACAGATGGA CGATGCTGTG 
ATGGACAACG TCAAGCAAAT CTTTGGCTTT GATAGCAACA AGAAGAACCT GGTGGACCCC 
TTTGTGGAAG TCAGCTTTGC TGGGAAAATG CTTTGCAGCA AGATCCTGGA GAAGACTGCT 
AACCCTCAGT GGAACCAGAA CATCACATTG CCTGCGATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACTCACA ATGACACTGT GGCTACCACC 
TACTTGGGTA TGTCGAAAAT CTCTGCCACT GGAGGAGAAA TAGAAGTGGA TGACAACCTG 
GGCTTCCTCC CCACTTTTGG GCCCTGCTAT GTGAACCTCT ATGGCAGTCC CCGAGAGTTC 
ACGGGCTTCC CAGATCCTTA TGCGGAGCTC AACACAGGAA AGGGAGAAGG TGTGGCATAT 
AGGGGCAGGC TTCTGTTGTC CCTGGAGACC AAACTGGTGG AGCACAGTGA ACAGAAGGTG 
GAAGACCTTC CTGCTGATGA CATCTTGAGA GTGGAGAAGT ACCTGCGGAG GCGCAAATAT 
TCCCTCTTTG CTGCCTTCTA CTCAGCCACA ATGCTGCAGG ATGTGGATGA TGCCATCCAG 
TTCGAGGTCA GCATTGGGAA CTACGGAAAC AAGTTTGACA CCACCTGCTT GCCGTTGGCC 
TCTACCACCC AATACAGCCG GGCAGTCTTT GATGGATGCC ACTACTATTA TTTGCCCTGG 
GGCAACGTGA AGCCTGTGGT AGTACTGTCC TCATACTGGG AAGACATCAG CCATCGAGTC 
GAGACTCAGA ACCAGCTCCT CAGGATCGCT GACCGACTGG AAGCTAACCT GGAGCAAGTC 
CACCTGGCTC TGAAGGCACA GTGTTCCTCT GAGGATGTGG ACGCCCTGGT GGCTCAGTTG 
ACAGATGAGC TCCTTGCAGA CTGCAGCCAG CCCCTGTGTG ACATCCATGA AATACCTTCT 
GCCACCCACC TTGATCAGTA CCTGTTCCGC CTCCGCTCCC GTCACCTGAG CCAGATCAAA 
GAAGCTGCCC TGGCCTTGAA GCTTGGCCAC AGTGAGCTCT CCACTGCCCT GGAGCAGGCC 
GAGGACTGGC TTCTGCATCT CCGTGCCCTG GCTGAGGAGC CCCAGAACAG CCTGCCAGAT 
ATCATCATCT GGATGCTGCA GGGTGACAAG CGTGTGGCCT ACCAACGGGT GCCTGCCCAT 
GAAGTCCTCT TCTCCCGGCG GGGCCCCAGC TACTGCGGCA GGAACTGTGG GAATCTGCAG 
ACCATCTTTC TGAAATATCC TATGGAAGGC ATGCCTGGGC CCCGGATGCC GGTGCAGATC 
CGGATCAAGC TCTGGTTTGG GCTCTCAGTG GATGAGAAGG AATTCAATCA GTTTGCAGAG 
GGGAAGCTCT CTGTCTTTGC TGAAACGTAT GAGAACCAGA CAAAGCTGGC GCTGGTCGGG 
AACTGGGGCA CGACGGGCCT CACCTACCCC AAGTTTTCTG ACGTCACAGG CAAGATCAAA 
CTACCGAAGG ATAGCTTCCG CCCCTCAGCT GGCTGGGCCT GGGCAGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACCCTGCT CCATGATGCT GACGCCGGTC ATCTGAGCTT TGTGGAGGAG 
GTGTTTGAGA ACCAGACTCG GCTACCTGGA GGCCAGTGGA TCTATATGAG TGACAACTAC 
ACCGACGTGA ATGGGGAGAA GGTGCTTCCA AAGGACGATA TTGAATGCCC GCTGGGTTGG 
AAGTGGGAAG ATGAAGAATG GTCCACAGAC CTCAATCGGG CTGTTGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATACC CCCAGACCGA AAGCCCAAAC ACTGGGTCCC TGTTGAGAAG 
ATGTATTACA CTCACAGGCG TCGACGCTGG GTCCGACTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCAC TGAAGAAGCA CAGGCAGGCA GAGGCTGAGG GCGAGGGCTG GGAATACGCC 
TCTCTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGATGCCTT TCGCCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCACTGGAG AAGACGGGGC CTGCAGCGGT GTTTGCCCTT 
GAGGGGGCCC TGGGCGGCAT GGTGGATGAC AAGAGCGAAG ATTCCATGTC TGTGTCTACC 
CTGAGCTTTG GCGTTAACAG ACCCACAATC TCCTGCATCT TTGACTATGG GAACCGCTAC 
CATCTGCGTT GCTACCTGTA CCAGGCCCGG GACCTGCCCG CCATGGACAA AGACTCCTTC 
TCTGATCCCT ATGCCATTGT CTCCTTCCTG CATCAAAGCC AGAAGACGGT GGTGGAGAAG 
AACACCCTGA ACCCCACCTG GGATCAAACC CTCATCTTCT ATGAGATAGA GATCTTTGGC 
GAGCCGGCCA GTGTCGCCGA GCGGCCACCC AGCATTGTGG TGGAGCTGTA TGACCATGAC 
ACCTATGGCG CTGACGAGTT CATGGGCCGC TGTATTTGTC AGCCAAGCCT GGAACGGATG 
CCCCGCCTGG CCTGGTTCCC ACTGACCAGG GGCAGCCAGC CTGCCGGAGA GCTGCTGGCA 
GCGTTCGAGC TTATCCAGAG AGAGAAGCCA GCCATCCATC ACATCCCTGG CTTTGAGATG 
CACGAAACTT CAAGGATTTT GGATGAGACG GAGGACACAG ACTTGCCTTA CCCACCACCT 
CAAAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCAGCTCT CCAGCGTACA 
GCCATCGAGA TCCTGGCCTG GGGCCTTCGG AACATAAAGA GCTATCAGAT GGCCAGCGTG 
TCTTCCCCCA GCCTCGTGGT GGAGTGTGGT GGGCAGACAG TGCAGTCCTG TGTCATCAGA 
AACCTTCGGA AGAACCCCAA CTTTGACGTC TGTACTCTGT TCATGGAAGT GATGTTACCC 
AGAGAGGACC TCTACTGTCC CCCTATTGTG GTCAAGGTCA TTGATAATCG CCAGTTTGGC 
CGCAGGCCAG TTGTGGGTCA GTGCACCATT CGCTCCCTGG AGAACTTCCT GTGTGACCCG 
TACTCAGCAG AGAGTCCATC CCCGCAAGGT GGCCCAGATG ACGTGAGCTT ACTCAGCCCT 
GGGGAAGACG TGCTCATTGA CATTGATGAC AAGGAGCCTC TCATCCCTGT CCAGGAGGAG 
GAGTTCATTG ACTGGTGGAG CAAATTCTTT GCTTCTATCG GGGAGAGGGA AAAGTGTGGT 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG GAGGCCTGTC CGACTTTTGT AATACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGACA CAGAAGATCC ATCTGTCATC GGAGAATTTA AGGGTCTCTT CAAAATTTAT 
CCCCTCCCAG AAGATCCAGC CATCCCTATG CCCCCGAGAC AATTCCATCA GCTGGCTGCT 
CAGGGTCCCC AGGAATGCTT GGTCCGGATT TACATCATCC GAGCATTTGG CTTACAGCCC 
AAGGACCCCA ATGGCAAGTG CGACCCTTAC ATCAAGATCT CTATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCTTGTACC CTGGAGCCTG TATTTGGAAA GATGTTTGAA 
CTCACCTGCA CTCTACCTCT GGAGAAGGAC TTGAAGATCA CACTCTATGA CTATGACCTG 
CTCTCCAAGG ATGAGAAGAT TGGGGAGACA GTCATTGATC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG CCTTCCACAG ACCTACTGTG TCTCTGGACC AAATCAGTGG 
AGAGATCAAC TCCGCCCTTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACAGA CCGAGTGACG TTTCAGGATA AGGAATACAC CATTCAGGAG 
ATAGAGGCTG GCAGACTCCC AAACCCACAC CTGGGCCCGG TGGAGGAACG CTTAGCCCTG 
CATGTCCTTC AACAACAAGG CTTGGTCCCT GAGCATGTGG AGTCTCGGCC TCTGTACAGT 
CCTCTGCAGC CAGATATTGA GCAGGGGAAG CTACAGATGT GGATTGACAT ATTTCCAAAG 
GTGCTGGGCC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAAAGCTCG AAGGTTTTTT 
CTGCGTTGTA TTATTTGGAA CACAAAAGAT GTGATCTTGG ATGACCTCAG CCTCACGGGG 
GAGAAGATGA GTGACATCTA TGTGAAAGGC TGGATGGTGG GATTCGAAGA ACACAAACAG 
AAGACAGATG TGCACTACCG TTCCCTGGGC GGGGAGGGCA ATTTCAACTG GAGGTTCGTG 
TTTCCCTTCG ACTATCTGCC CGCTGAGCAA GTCTGTGCTG TTGCCAAGAA GGATGCTTTC 
TGGAGACTCG ACAAGACAGA GAGCAAGATC CCAGCACGTG TGGTCTTCCA GATTTGGGAC 
AATGACAAGT TCTCCTTTGA TGACTTTCTG GGCTCCCTGC AGCTGGATCT CAACCGAATG 
CCCAAGCCAG CCAAGACGGC TGAGAAGTGC TCCTTGGACC AGCTGGATGA CACCTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAGACAGTGA AAGGATGGTG GCCTTGTGTG 
ACAGAGGAGG GCGCGAAGAA GATCCTGGCG GGGAAGCTGG AAATGACCTT GGAGATCGTT 
GCAGAAAGTG AACATGAGGA GCGGCCTGCT GGCCAGGGTC GGGATGAACC CAACATGAAT 
CCAAAGCTAG AAGATCCCAG GCGCCCTGAC ACCTCTTTCC TGTGGTTCAC CTCACCATAC 
AAGACCATGA AGTTCATCTT GTGGCGACGA TTCCGGTGTG CCATCATCCT CTTCATCATC 
CTCTTCATCC TCCTGCTCTT CCTGGGTGTC TTTGTCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT TAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Dysf
Entrez Gene ID	312492
Full Name	dysferlin
General protein information	Preferred Names dysferlin Names dysferlin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34

Homo sapiens (human)	DYSF	NP_001124454.1
	DYSF	XP_002799336.1
Caenorhabditis elegans (roundworm)	fer-1	NP_492337.1
Caenorhabditis elegans (roundworm)	ferl-1	NP_491602.2
Rattus norvegicus (Norway rat)	Dysf	NP_001101339.1
Danio rerio (zebrafish)	dysf	NP_001244122.1
Mus musculus (house mouse)	Dysf	NP_067444.2
Canis lupus familiaris (dog)	DYSF	XP_003432278.1
Bos taurus (cattle)	DYSF	NP_001095960.1
Pan troglodytes (chimpanzee)	DYSF	XP_003309136.1
Gallus gallus (chicken)	DYSF	XP_423126.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Dysf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

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