dysf cDNA ORF clone, Danio rerio(zebrafish)

The following dysf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dysf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa143308 XM_021477555.1
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa143309 XM_021477556.1
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa143310 XM_021477557.1
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa143311 XM_021477558.1
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa143312 XM_021477560.1
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa43390 XM_021477559.1
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa59639 XM_017356879.2
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa35448 NM_001257193.1
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa12912 XM_005172291.4
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa12914 XM_005172293.4
Latest version!
Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
ODa12914 XM_005172293.3 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa12912 XM_005172291.3 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa59639 XM_017356879.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa143308
    Clone ID Related Accession (Same CDS sequence) XM_021477555.1
    Accession Version XM_021477555.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA
    ATCCCTGGAC AGGCAGATGG CACCAGCTCT GCCATTATTA ACATCTCGCC TTCTCAGTCC
    AGCCTTCATC AGGATGAATT TATGGACTGG TGGAGCAAGT TTTATGCCTC CATGGGTGAG
    AGGACCAAGT GTGGGAGTTA TCTGGAGAAA GGGTTCGATA TGCTGCAGGT GTATGACAGA
    GAACTGGAGA AGACTGAAGG CTTTGAGCAA TTTGCTGATT TCTGTCAAAC GTTTAAACTT
    TACCGTGGAA GAACCCAGAA TGACAGCGAA GATCCTTCCG TTGTTGGAGA GTTCAAGGGT
    TCGTTTAAGA TCTATCCATT GCCTGATGAT CCCTCTATGC CTGTACCCAC ACGTCAGTTT
    CGCAAACTCC CCCCGAACGG TATAGAGGAG TGCTTGGTAC GCATCTACAT CATCCAGGCT
    TTTGGGTTAC AGCCCAAAGA TGCCAATGGC AAGTGTGACC CCTATGTTAA GATCACGCTT
    GGCAAGAAAT CAGTCGATGA CCACGACAAT TACATACCCT GTACTTTGGA GCCCGTCTTT
    GGAAAGATGT TTGAGCTGAC CTGCTGTCTG CCTCTTGAGA AAGACCTGAA GATTACACTC
    TATGATTACG ACATGGTGAC TAAAGATGAG AAAATCGGAG AGACCATCAT TGATTTGGAG
    AACCGCTTCT TGTCTCGTCA TGGTGCTCGC TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA
    GGAATTAACC AGTGGCGAGA TCAACTTAAA CCATCTCAGC TACTTCATCG TCTGTGTGAG
    AGGCGAAACT ACAAACTCCC CGTCTACAAG CAGGATCGAA TTTACTTCAG AGGACATGAA
    TACACTGTCG CAGACCTTGA TGAAGGGAAA CCTCTTAACC CTCACTTGGG ACCAGTGATG
    GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG CATGGTTTGG TGCCTGAACA TGTCGAGACG
    CGACCCCTCT ACAGCCCCCT TCAGCCAGAT ATAGAACAGG GGCGACTACA AATGTGGGTG
    GACCTGTTCC CTAAGTCTTT AGGCCCTCCA GGTCCTCCAT TCAACATCAC CCCACGCAAA
    GCTAAAAAGT TCTTCCTGCG CTGTATTATA TGGAACACAA GAGATGTAAT CTTGGATGAT
    GTCAGCGTTA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GCACGGCCAT
    GAGGAGAACA AGCAGAAAAC AGACGTGCAC TACCGATCGC TGGGTGGTGA GGGTAACTTT
    AACTGGCGCT TCCTCTTCCC CTTCCACTAT TTACCTGCGG AACAACTGTG CACCATTGAT
    AAAAAGGAGC ACTTTTGGAG TCTGGATAAA GTTGAAACGA AGCTTCCTCC TAAACTTACT
    ATCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTCGGCTC TTTGGAGATG
    GATCTGAACC ACATGCTGAG TCCTGCGAAG TCTCCAGAGA AGTGTGGTCT TGAGATGCTC
    ACTCAGTCCC AGGACAAACT GGTCTCTTTG TTTGAGCAGA AAACGGTGAA AGGCTGGTGG
    CCGTGTGTCT GTGACAAAGA AGACAAGAAG ATACTTGCTG GAAAGGTGGA GATGTCTCTG
    GAAATAGTCT GTGAACAGGA GCAGGATGAG AGGCCTGCAG GACTCGGCCG AGATGAGCCC
    AACATGAATC CCCATCTAGA GGAGCCTCAG CGTCCCGAGA CATCATTCTT ATGGTTCTCG
    TCCCCATATA AGACGTTTAG GTTCATTGTG TGGAGGAGGT TTAAGTGGCT TATTCTGGGC
    CTCTTTATAC TCTTTTTCAT TATTCTCTTC CTTGGAGTAT TTCTCTACGC CTTCCCTAAC
    TATGCTGCTA TGAAGATGGT TGGACCCTTC AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333230.1
    CDS307..6663
    Translation

    Target ORF information:

    RefSeq Version XM_021477555.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA
    ATCCCTGGAC AGGCAGATGG CACCAGCTCT GCCATTATTA ACATCTCGCC TTCTCAGTCC
    AGCCTTCATC AGGATGAATT TATGGACTGG TGGAGCAAGT TTTATGCCTC CATGGGTGAG
    AGGACCAAGT GTGGGAGTTA TCTGGAGAAA GGGTTCGATA TGCTGCAGGT GTATGACAGA
    GAACTGGAGA AGACTGAAGG CTTTGAGCAA TTTGCTGATT TCTGTCAAAC GTTTAAACTT
    TACCGTGGAA GAACCCAGAA TGACAGCGAA GATCCTTCCG TTGTTGGAGA GTTCAAGGGT
    TCGTTTAAGA TCTATCCATT GCCTGATGAT CCCTCTATGC CTGTACCCAC ACGTCAGTTT
    CGCAAACTCC CCCCGAACGG TATAGAGGAG TGCTTGGTAC GCATCTACAT CATCCAGGCT
    TTTGGGTTAC AGCCCAAAGA TGCCAATGGC AAGTGTGACC CCTATGTTAA GATCACGCTT
    GGCAAGAAAT CAGTCGATGA CCACGACAAT TACATACCCT GTACTTTGGA GCCCGTCTTT
    GGAAAGATGT TTGAGCTGAC CTGCTGTCTG CCTCTTGAGA AAGACCTGAA GATTACACTC
    TATGATTACG ACATGGTGAC TAAAGATGAG AAAATCGGAG AGACCATCAT TGATTTGGAG
    AACCGCTTCT TGTCTCGTCA TGGTGCTCGC TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA
    GGAATTAACC AGTGGCGAGA TCAACTTAAA CCATCTCAGC TACTTCATCG TCTGTGTGAG
    AGGCGAAACT ACAAACTCCC CGTCTACAAG CAGGATCGAA TTTACTTCAG AGGACATGAA
    TACACTGTCG CAGACCTTGA TGAAGGGAAA CCTCTTAACC CTCACTTGGG ACCAGTGATG
    GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG CATGGTTTGG TGCCTGAACA TGTCGAGACG
    CGACCCCTCT ACAGCCCCCT TCAGCCAGAT ATAGAACAGG GGCGACTACA AATGTGGGTG
    GACCTGTTCC CTAAGTCTTT AGGCCCTCCA GGTCCTCCAT TCAACATCAC CCCACGCAAA
    GCTAAAAAGT TCTTCCTGCG CTGTATTATA TGGAACACAA GAGATGTAAT CTTGGATGAT
    GTCAGCGTTA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GCACGGCCAT
    GAGGAGAACA AGCAGAAAAC AGACGTGCAC TACCGATCGC TGGGTGGTGA GGGTAACTTT
    AACTGGCGCT TCCTCTTCCC CTTCCACTAT TTACCTGCGG AACAACTGTG CACCATTGAT
    AAAAAGGAGC ACTTTTGGAG TCTGGATAAA GTTGAAACGA AGCTTCCTCC TAAACTTACT
    ATCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTCGGCTC TTTGGAGATG
    GATCTGAACC ACATGCTGAG TCCTGCGAAG TCTCCAGAGA AGTGTGGTCT TGAGATGCTC
    ACTCAGTCCC AGGACAAACT GGTCTCTTTG TTTGAGCAGA AAACGGTGAA AGGCTGGTGG
    CCGTGTGTCT GTGACAAAGA AGACAAGAAG ATACTTGCTG GAAAGGTGGA GATGTCTCTG
    GAAATAGTCT GTGAACAGGA GCAGGATGAG AGGCCTGCAG GACTCGGCCG AGATGAGCCC
    AACATGAATC CCCATCTAGA GGAGCCTCAG CGTCCCGAGA CATCATTCTT ATGGTTCTCG
    TCCCCATATA AGACGTTTAG GTTCATTGTG TGGAGGAGGT TTAAGTGGCT TATTCTGGGC
    CTCTTTATAC TCTTTTTCAT TATTCTCTTC CTTGGAGTAT TTCTCTACGC CTTCCCTAAC
    TATGCTGCTA TGAAGATGGT TGGACCCTTC AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143309
    Clone ID Related Accession (Same CDS sequence) XM_021477556.1
    Accession Version XM_021477556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACGG
    CCAGACGAGT CCGAGCGTGA GGGCTGGGAA TATGCCTCTT TATTTGGCTG GAAGTTTCAC
    CTCAAGCAGA AGAAAACCGA CTTGTTCCGT CGCCGGCGCT GGAGACGTCG AATGGAGCCA
    TTAGAAAAGA CGGGGCCGGC GGCCATCTTT GCCTTGGAGT GTTCGCTGAG CAGCTTTACT
    GATGAAAAGA ATGACGATAA GTCAGTCACT ACAACATTTG GTGTCAGCCG GCCGACTATC
    TCCTGCTTCT TTGATAGTGG GACCCTGTAT CATTTGCGCT GTTACTTGTA TCAGGCCAGA
    GACCTGCTGG CTATGGACAA GGACAGTTTC TCGGATGCCT ATGCCGTTGT ATCGTTCCTT
    CATCAGAGTC AGAAGACGGT GACTGTTCGA AACACACTCA ACCCCACCTG GGACCAGACC
    CTCATTTTCT ACGAAGTGGA AATTTTTGGA GACCATCTTG TGACTGAGAG GAGCCCTCCA
    AACATTGTGG TGGAGCTGTA CGATCAGGAC ACCTATGGAG CTGATGAGTT CATGGGCCGC
    TGTGTTTGCC AACCATCAAT GGCATCTTCC CCTCGCCTCG CCTGGTATCC CATCCAGAAA
    GCAGGGAATT CTGCAGGAGA TCTGCTTGCT GCTTTTGAAC TGATCCGCAG AGATAAACCT
    GCAGTTCATC ACATCCCGGG TCAAGAGGGA GATATTATAC CACCCTCACA TATCATGGAT
    GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG AATCAACAGC AATGGCCAGA GGACTCTGAT
    TTGCCCTATC CACCACCTCA GAGGGAGCCA AACGTCTACA TGGTGCCACA GGGGATAAAA
    CCTGTCCTAC AGCGCACTGC TATTGAGATA CTGGCATGGG GTTTGCGTAA CCTGAAGAGT
    TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT CTTCAGGTGG AATGTGGCGG ATCTGTGGTT
    CAGTCGTGCG TCATTCGAAA TATTAAGAAG AACCCTAATT TTGACGTCAA CATCCTGTTC
    ATTGATGTCC GATTGCCTAA GGAAGAGCTC TACATGCCCC CATTGGTGAT CAAGGTTATA
    GACAACCGGC AGTTTGGCAG GAAGCCTGTG GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT
    GAGTTCTACT TCCAGCCGGA AAGTCTGGAG AGAGACTCAA CAGAAGAGGA ATTTGAAGTT
    ATTGCTCACA CACCTAGAGA TGATGTTCTG GTTGATATTG ATGACAAGGA GCCTCTAATC
    CCTGGACAGG CAGATGGCAC CAGCTCTGCC ATTATTAACA TCTCGCCTTC TCAGTCCAGC
    CTTCATCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333231.1
    CDS307..6660
    Translation

    Target ORF information:

    RefSeq Version XM_021477556.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACGG
    CCAGACGAGT CCGAGCGTGA GGGCTGGGAA TATGCCTCTT TATTTGGCTG GAAGTTTCAC
    CTCAAGCAGA AGAAAACCGA CTTGTTCCGT CGCCGGCGCT GGAGACGTCG AATGGAGCCA
    TTAGAAAAGA CGGGGCCGGC GGCCATCTTT GCCTTGGAGT GTTCGCTGAG CAGCTTTACT
    GATGAAAAGA ATGACGATAA GTCAGTCACT ACAACATTTG GTGTCAGCCG GCCGACTATC
    TCCTGCTTCT TTGATAGTGG GACCCTGTAT CATTTGCGCT GTTACTTGTA TCAGGCCAGA
    GACCTGCTGG CTATGGACAA GGACAGTTTC TCGGATGCCT ATGCCGTTGT ATCGTTCCTT
    CATCAGAGTC AGAAGACGGT GACTGTTCGA AACACACTCA ACCCCACCTG GGACCAGACC
    CTCATTTTCT ACGAAGTGGA AATTTTTGGA GACCATCTTG TGACTGAGAG GAGCCCTCCA
    AACATTGTGG TGGAGCTGTA CGATCAGGAC ACCTATGGAG CTGATGAGTT CATGGGCCGC
    TGTGTTTGCC AACCATCAAT GGCATCTTCC CCTCGCCTCG CCTGGTATCC CATCCAGAAA
    GCAGGGAATT CTGCAGGAGA TCTGCTTGCT GCTTTTGAAC TGATCCGCAG AGATAAACCT
    GCAGTTCATC ACATCCCGGG TCAAGAGGGA GATATTATAC CACCCTCACA TATCATGGAT
    GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG AATCAACAGC AATGGCCAGA GGACTCTGAT
    TTGCCCTATC CACCACCTCA GAGGGAGCCA AACGTCTACA TGGTGCCACA GGGGATAAAA
    CCTGTCCTAC AGCGCACTGC TATTGAGATA CTGGCATGGG GTTTGCGTAA CCTGAAGAGT
    TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT CTTCAGGTGG AATGTGGCGG ATCTGTGGTT
    CAGTCGTGCG TCATTCGAAA TATTAAGAAG AACCCTAATT TTGACGTCAA CATCCTGTTC
    ATTGATGTCC GATTGCCTAA GGAAGAGCTC TACATGCCCC CATTGGTGAT CAAGGTTATA
    GACAACCGGC AGTTTGGCAG GAAGCCTGTG GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT
    GAGTTCTACT TCCAGCCGGA AAGTCTGGAG AGAGACTCAA CAGAAGAGGA ATTTGAAGTT
    ATTGCTCACA CACCTAGAGA TGATGTTCTG GTTGATATTG ATGACAAGGA GCCTCTAATC
    CCTGGACAGG CAGATGGCAC CAGCTCTGCC ATTATTAACA TCTCGCCTTC TCAGTCCAGC
    CTTCATCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143310
    Clone ID Related Accession (Same CDS sequence) XM_021477557.1
    Accession Version XM_021477557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGG CAGATGGCAC CAGCTCTGCC
    ATTATTAACA TCTCGCCTTC TCAGTCCAGC CTTCATCAGG ATGAATTTAT GGACTGGTGG
    AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG ACCAAGTGTG GGAGTTATCT GGAGAAAGGG
    TTCGATATGC TGCAGGTGTA TGACAGAGAA CTGGAGAAGA CTGAAGGCTT TGAGCAATTT
    GCTGATTTCT GTCAAACGTT TAAACTTTAC CGTGGAAGAA CCCAGAATGA CAGCGAAGAT
    CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG TTTAAGATCT ATCCATTGCC TGATGATCCC
    TCTATGCCTG TACCCACACG TCAGTTTCGC AAACTCCCCC CGAACGGTAT AGAGGAGTGC
    TTGGTACGCA TCTACATCAT CCAGGCTTTT GGGTTACAGC CCAAAGATGC CAATGGCAAG
    TGTGACCCCT ATGTTAAGAT CACGCTTGGC AAGAAATCAG TCGATGACCA CGACAATTAC
    ATACCCTGTA CTTTGGAGCC CGTCTTTGGA AAGATGTTTG AGCTGACCTG CTGTCTGCCT
    CTTGAGAAAG ACCTGAAGAT TACACTCTAT GATTACGACA TGGTGACTAA AGATGAGAAA
    ATCGGAGAGA CCATCATTGA TTTGGAGAAC CGCTTCTTGT CTCGTCATGG TGCTCGCTGC
    GGCCTTCCGC AGTCCTACTG TCTGTCAGGA ATTAACCAGT GGCGAGATCA ACTTAAACCA
    TCTCAGCTAC TTCATCGTCT GTGTGAGAGG CGAAACTACA AACTCCCCGT CTACAAGCAG
    GATCGAATTT ACTTCAGAGG ACATGAATAC ACTGTCGCAG ACCTTGATGA AGGGAAACCT
    CTTAACCCTC ACTTGGGACC AGTGATGGAG CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT
    GGTTTGGTGC CTGAACATGT CGAGACGCGA CCCCTCTACA GCCCCCTTCA GCCAGATATA
    GAACAGGGGC GACTACAAAT GTGGGTGGAC CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT
    CCTCCATTCA ACATCACCCC ACGCAAAGCT AAAAAGTTCT TCCTGCGCTG TATTATATGG
    AACACAAGAG ATGTAATCTT GGATGATGTC AGCGTTACCG GGGAGAAGAT GAGTGACATT
    TATGTGAAAG GCTGGATGCA CGGCCATGAG GAGAACAAGC AGAAAACAGA CGTGCACTAC
    CGATCGCTGG GTGGTGAGGG TAACTTTAAC TGGCGCTTCC TCTTCCCCTT CCACTATTTA
    CCTGCGGAAC AACTGTGCAC CATTGATAAA AAGGAGCACT TTTGGAGTCT GGATAAAGTT
    GAAACGAAGC TTCCTCCTAA ACTTACTATC CAGATTTGGG ACAATGACAA GTTCTCCTTT
    GATGATTTCC TCGGCTCTTT GGAGATGGAT CTGAACCACA TGCTGAGTCC TGCGAAGTCT
    CCAGAGAAGT GTGGTCTTGA GATGCTCACT CAGTCCCAGG ACAAACTGGT CTCTTTGTTT
    GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG TGTGTCTGTG ACAAAGAAGA CAAGAAGATA
    CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA ATAGTCTGTG AACAGGAGCA GGATGAGAGG
    CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC ATGAATCCCC ATCTAGAGGA GCCTCAGCGT
    CCCGAGACAT CATTCTTATG GTTCTCGTCC CCATATAAGA CGTTTAGGTT CATTGTGTGG
    AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC TTTATACTCT TTTTCATTAT TCTCTTCCTT
    GGAGTATTTC TCTACGCCTT CCCTAACTAT GCTGCTATGA AGATGGTTGG ACCCTTCAGT
    GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333232.1
    CDS307..6630
    Translation

    Target ORF information:

    RefSeq Version XM_021477557.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGG CAGATGGCAC CAGCTCTGCC
    ATTATTAACA TCTCGCCTTC TCAGTCCAGC CTTCATCAGG ATGAATTTAT GGACTGGTGG
    AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG ACCAAGTGTG GGAGTTATCT GGAGAAAGGG
    TTCGATATGC TGCAGGTGTA TGACAGAGAA CTGGAGAAGA CTGAAGGCTT TGAGCAATTT
    GCTGATTTCT GTCAAACGTT TAAACTTTAC CGTGGAAGAA CCCAGAATGA CAGCGAAGAT
    CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG TTTAAGATCT ATCCATTGCC TGATGATCCC
    TCTATGCCTG TACCCACACG TCAGTTTCGC AAACTCCCCC CGAACGGTAT AGAGGAGTGC
    TTGGTACGCA TCTACATCAT CCAGGCTTTT GGGTTACAGC CCAAAGATGC CAATGGCAAG
    TGTGACCCCT ATGTTAAGAT CACGCTTGGC AAGAAATCAG TCGATGACCA CGACAATTAC
    ATACCCTGTA CTTTGGAGCC CGTCTTTGGA AAGATGTTTG AGCTGACCTG CTGTCTGCCT
    CTTGAGAAAG ACCTGAAGAT TACACTCTAT GATTACGACA TGGTGACTAA AGATGAGAAA
    ATCGGAGAGA CCATCATTGA TTTGGAGAAC CGCTTCTTGT CTCGTCATGG TGCTCGCTGC
    GGCCTTCCGC AGTCCTACTG TCTGTCAGGA ATTAACCAGT GGCGAGATCA ACTTAAACCA
    TCTCAGCTAC TTCATCGTCT GTGTGAGAGG CGAAACTACA AACTCCCCGT CTACAAGCAG
    GATCGAATTT ACTTCAGAGG ACATGAATAC ACTGTCGCAG ACCTTGATGA AGGGAAACCT
    CTTAACCCTC ACTTGGGACC AGTGATGGAG CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT
    GGTTTGGTGC CTGAACATGT CGAGACGCGA CCCCTCTACA GCCCCCTTCA GCCAGATATA
    GAACAGGGGC GACTACAAAT GTGGGTGGAC CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT
    CCTCCATTCA ACATCACCCC ACGCAAAGCT AAAAAGTTCT TCCTGCGCTG TATTATATGG
    AACACAAGAG ATGTAATCTT GGATGATGTC AGCGTTACCG GGGAGAAGAT GAGTGACATT
    TATGTGAAAG GCTGGATGCA CGGCCATGAG GAGAACAAGC AGAAAACAGA CGTGCACTAC
    CGATCGCTGG GTGGTGAGGG TAACTTTAAC TGGCGCTTCC TCTTCCCCTT CCACTATTTA
    CCTGCGGAAC AACTGTGCAC CATTGATAAA AAGGAGCACT TTTGGAGTCT GGATAAAGTT
    GAAACGAAGC TTCCTCCTAA ACTTACTATC CAGATTTGGG ACAATGACAA GTTCTCCTTT
    GATGATTTCC TCGGCTCTTT GGAGATGGAT CTGAACCACA TGCTGAGTCC TGCGAAGTCT
    CCAGAGAAGT GTGGTCTTGA GATGCTCACT CAGTCCCAGG ACAAACTGGT CTCTTTGTTT
    GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG TGTGTCTGTG ACAAAGAAGA CAAGAAGATA
    CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA ATAGTCTGTG AACAGGAGCA GGATGAGAGG
    CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC ATGAATCCCC ATCTAGAGGA GCCTCAGCGT
    CCCGAGACAT CATTCTTATG GTTCTCGTCC CCATATAAGA CGTTTAGGTT CATTGTGTGG
    AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC TTTATACTCT TTTTCATTAT TCTCTTCCTT
    GGAGTATTTC TCTACGCCTT CCCTAACTAT GCTGCTATGA AGATGGTTGG ACCCTTCAGT
    GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143311
    Clone ID Related Accession (Same CDS sequence) XM_021477558.1
    Accession Version XM_021477558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG
    GATGAGCCAG AGGACTCTGA TTTGCCCTAT CCACCACCTC AGAGGGAGCC AAACGTCTAC
    ATGGTGCCAC AGGGGATAAA ACCTGTCCTA CAGCGCACTG CTATTGAGAT ACTGGCATGG
    GGTTTGCGTA ACCTGAAGAG TTTCCAGCTG GCCAGTGTCA CGTCTCCCAG TCTTCAGGTG
    GAATGTGGCG GATCTGTGGT TCAGTCGTGC GTCATTCGAA ATATTAAGAA GAACCCTAAT
    TTTGACGTCA ACATCCTGTT CATTGATGTC CGATTGCCTA AGGAAGAGCT CTACATGCCC
    CCATTGGTGA TCAAGGTTAT AGACAACCGG CAGTTTGGCA GGAAGCCTGT GGTGGGCCAG
    TGTTCAGTCC ACTCGCTCAA TGAGTTCTAC TTCCAGCCGG AAAGTCTGGA GAGAGACTCA
    ACAGAAGAGG AATTTGAAGT TATTGCTCAC ACACCTAGAG ATGATGTTCT GGTTGATATT
    GATGACAAGG AGCCTCTAAT CCCTGGACAG GCAGATGGCA CCAGCTCTGC CATTATTAAC
    ATCTCGCCTT CTCAGTCCAG CCTTCATCAG GATGAATTTA TGGACTGGTG GAGCAAGTTT
    TATGCCTCCA TGGGTGAGAG GACCAAGTGT GGGAGTTATC TGGAGAAAGG GTTCGATATG
    CTGCAGGTGT ATGACAGAGA ACTGGAGAAG ACTGAAGGCT TTGAGCAATT TGCTGATTTC
    TGTCAAACGT TTAAACTTTA CCGTGGAAGA ACCCAGAATG ACAGCGAAGA TCCTTCCGTT
    GTTGGAGAGT TCAAGGGTTC GTTTAAGATC TATCCATTGC CTGATGATCC CTCTATGCCT
    GTACCCACAC GTCAGTTTCG CAAACTCCCC CCGAACGGTA TAGAGGAGTG CTTGGTACGC
    ATCTACATCA TCCAGGCTTT TGGGTTACAG CCCAAAGATG CCAATGGCAA GTGTGACCCC
    TATGTTAAGA TCACGCTTGG CAAGAAATCA GTCGATGACC ACGACAATTA CATACCCTGT
    ACTTTGGAGC CCGTCTTTGG AAAGATGTTT GAGCTGACCT GCTGTCTGCC TCTTGAGAAA
    GACCTGAAGA TTACACTCTA TGATTACGAC ATGGTGACTA AAGATGAGAA AATCGGAGAG
    ACCATCATTG ATTTGGAGAA CCGCTTCTTG TCTCGTCATG GTGCTCGCTG CGGCCTTCCG
    CAGTCCTACT GTCTGTCAGG AATTAACCAG TGGCGAGATC AACTTAAACC ATCTCAGCTA
    CTTCATCGTC TGTGTGAGAG GCGAAACTAC AAACTCCCCG TCTACAAGCA GGATCGAATT
    TACTTCAGAG GACATGAATA CACTGTCGCA GACCTTGATG AAGGGAAACC TCTTAACCCT
    CACTTGGGAC CAGTGATGGA GCGTCTGGCT CTGTTGGTGT TGAGGAGGCA TGGTTTGGTG
    CCTGAACATG TCGAGACGCG ACCCCTCTAC AGCCCCCTTC AGCCAGATAT AGAACAGGGG
    CGACTACAAA TGTGGGTGGA CCTGTTCCCT AAGTCTTTAG GCCCTCCAGG TCCTCCATTC
    AACATCACCC CACGCAAAGC TAAAAAGTTC TTCCTGCGCT GTATTATATG GAACACAAGA
    GATGTAATCT TGGATGATGT CAGCGTTACC GGGGAGAAGA TGAGTGACAT TTATGTGAAA
    GGCTGGATGC ACGGCCATGA GGAGAACAAG CAGAAAACAG ACGTGCACTA CCGATCGCTG
    GGTGGTGAGG GTAACTTTAA CTGGCGCTTC CTCTTCCCCT TCCACTATTT ACCTGCGGAA
    CAACTGTGCA CCATTGATAA AAAGGAGCAC TTTTGGAGTC TGGATAAAGT TGAAACGAAG
    CTTCCTCCTA AACTTACTAT CCAGATTTGG GACAATGACA AGTTCTCCTT TGATGATTTC
    CTCGGCTCTT TGGAGATGGA TCTGAACCAC ATGCTGAGTC CTGCGAAGTC TCCAGAGAAG
    TGTGGTCTTG AGATGCTCAC TCAGTCCCAG GACAAACTGG TCTCTTTGTT TGAGCAGAAA
    ACGGTGAAAG GCTGGTGGCC GTGTGTCTGT GACAAAGAAG ACAAGAAGAT ACTTGCTGGA
    AAGGTGGAGA TGTCTCTGGA AATAGTCTGT GAACAGGAGC AGGATGAGAG GCCTGCAGGA
    CTCGGCCGAG ATGAGCCCAA CATGAATCCC CATCTAGAGG AGCCTCAGCG TCCCGAGACA
    TCATTCTTAT GGTTCTCGTC CCCATATAAG ACGTTTAGGT TCATTGTGTG GAGGAGGTTT
    AAGTGGCTTA TTCTGGGCCT CTTTATACTC TTTTTCATTA TTCTCTTCCT TGGAGTATTT
    CTCTACGCCT TCCCTAACTA TGCTGCTATG AAGATGGTTG GACCCTTCAG TGGACTAGGA
    AAGGGAACAC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333233.1
    CDS307..6621
    Translation

    Target ORF information:

    RefSeq Version XM_021477558.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG
    GATGAGCCAG AGGACTCTGA TTTGCCCTAT CCACCACCTC AGAGGGAGCC AAACGTCTAC
    ATGGTGCCAC AGGGGATAAA ACCTGTCCTA CAGCGCACTG CTATTGAGAT ACTGGCATGG
    GGTTTGCGTA ACCTGAAGAG TTTCCAGCTG GCCAGTGTCA CGTCTCCCAG TCTTCAGGTG
    GAATGTGGCG GATCTGTGGT TCAGTCGTGC GTCATTCGAA ATATTAAGAA GAACCCTAAT
    TTTGACGTCA ACATCCTGTT CATTGATGTC CGATTGCCTA AGGAAGAGCT CTACATGCCC
    CCATTGGTGA TCAAGGTTAT AGACAACCGG CAGTTTGGCA GGAAGCCTGT GGTGGGCCAG
    TGTTCAGTCC ACTCGCTCAA TGAGTTCTAC TTCCAGCCGG AAAGTCTGGA GAGAGACTCA
    ACAGAAGAGG AATTTGAAGT TATTGCTCAC ACACCTAGAG ATGATGTTCT GGTTGATATT
    GATGACAAGG AGCCTCTAAT CCCTGGACAG GCAGATGGCA CCAGCTCTGC CATTATTAAC
    ATCTCGCCTT CTCAGTCCAG CCTTCATCAG GATGAATTTA TGGACTGGTG GAGCAAGTTT
    TATGCCTCCA TGGGTGAGAG GACCAAGTGT GGGAGTTATC TGGAGAAAGG GTTCGATATG
    CTGCAGGTGT ATGACAGAGA ACTGGAGAAG ACTGAAGGCT TTGAGCAATT TGCTGATTTC
    TGTCAAACGT TTAAACTTTA CCGTGGAAGA ACCCAGAATG ACAGCGAAGA TCCTTCCGTT
    GTTGGAGAGT TCAAGGGTTC GTTTAAGATC TATCCATTGC CTGATGATCC CTCTATGCCT
    GTACCCACAC GTCAGTTTCG CAAACTCCCC CCGAACGGTA TAGAGGAGTG CTTGGTACGC
    ATCTACATCA TCCAGGCTTT TGGGTTACAG CCCAAAGATG CCAATGGCAA GTGTGACCCC
    TATGTTAAGA TCACGCTTGG CAAGAAATCA GTCGATGACC ACGACAATTA CATACCCTGT
    ACTTTGGAGC CCGTCTTTGG AAAGATGTTT GAGCTGACCT GCTGTCTGCC TCTTGAGAAA
    GACCTGAAGA TTACACTCTA TGATTACGAC ATGGTGACTA AAGATGAGAA AATCGGAGAG
    ACCATCATTG ATTTGGAGAA CCGCTTCTTG TCTCGTCATG GTGCTCGCTG CGGCCTTCCG
    CAGTCCTACT GTCTGTCAGG AATTAACCAG TGGCGAGATC AACTTAAACC ATCTCAGCTA
    CTTCATCGTC TGTGTGAGAG GCGAAACTAC AAACTCCCCG TCTACAAGCA GGATCGAATT
    TACTTCAGAG GACATGAATA CACTGTCGCA GACCTTGATG AAGGGAAACC TCTTAACCCT
    CACTTGGGAC CAGTGATGGA GCGTCTGGCT CTGTTGGTGT TGAGGAGGCA TGGTTTGGTG
    CCTGAACATG TCGAGACGCG ACCCCTCTAC AGCCCCCTTC AGCCAGATAT AGAACAGGGG
    CGACTACAAA TGTGGGTGGA CCTGTTCCCT AAGTCTTTAG GCCCTCCAGG TCCTCCATTC
    AACATCACCC CACGCAAAGC TAAAAAGTTC TTCCTGCGCT GTATTATATG GAACACAAGA
    GATGTAATCT TGGATGATGT CAGCGTTACC GGGGAGAAGA TGAGTGACAT TTATGTGAAA
    GGCTGGATGC ACGGCCATGA GGAGAACAAG CAGAAAACAG ACGTGCACTA CCGATCGCTG
    GGTGGTGAGG GTAACTTTAA CTGGCGCTTC CTCTTCCCCT TCCACTATTT ACCTGCGGAA
    CAACTGTGCA CCATTGATAA AAAGGAGCAC TTTTGGAGTC TGGATAAAGT TGAAACGAAG
    CTTCCTCCTA AACTTACTAT CCAGATTTGG GACAATGACA AGTTCTCCTT TGATGATTTC
    CTCGGCTCTT TGGAGATGGA TCTGAACCAC ATGCTGAGTC CTGCGAAGTC TCCAGAGAAG
    TGTGGTCTTG AGATGCTCAC TCAGTCCCAG GACAAACTGG TCTCTTTGTT TGAGCAGAAA
    ACGGTGAAAG GCTGGTGGCC GTGTGTCTGT GACAAAGAAG ACAAGAAGAT ACTTGCTGGA
    AAGGTGGAGA TGTCTCTGGA AATAGTCTGT GAACAGGAGC AGGATGAGAG GCCTGCAGGA
    CTCGGCCGAG ATGAGCCCAA CATGAATCCC CATCTAGAGG AGCCTCAGCG TCCCGAGACA
    TCATTCTTAT GGTTCTCGTC CCCATATAAG ACGTTTAGGT TCATTGTGTG GAGGAGGTTT
    AAGTGGCTTA TTCTGGGCCT CTTTATACTC TTTTTCATTA TTCTCTTCCT TGGAGTATTT
    CTCTACGCCT TCCCTAACTA TGCTGCTATG AAGATGGTTG GACCCTTCAG TGGACTAGGA
    AAGGGAACAC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143312
    Clone ID Related Accession (Same CDS sequence) XM_021477560.1
    Accession Version XM_021477560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA
    ATCCCTGGAC AGGATGAATT TATGGACTGG TGGAGCAAGT TTTATGCCTC CATGGGTGAG
    AGGACCAAGT GTGGGAGTTA TCTGGAGAAA GGGTTCGATA TGCTGCAGGT GTATGACAGA
    GAACTGGAGA AGACTGAAGG CTTTGAGCAA TTTGCTGATT TCTGTCAAAC GTTTAAACTT
    TACCGTGGAA GAACCCAGAA TGACAGCGAA GATCCTTCCG TTGTTGGAGA GTTCAAGGGT
    TCGTTTAAGA TCTATCCATT GCCTGATGAT CCCTCTATGC CTGTACCCAC ACGTCAGTTT
    CGCAAACTCC CCCCGAACGG TATAGAGGAG TGCTTGGTAC GCATCTACAT CATCCAGGCT
    TTTGGGTTAC AGCCCAAAGA TGCCAATGGC AAGTGTGACC CCTATGTTAA GATCACGCTT
    GGCAAGAAAT CAGTCGATGA CCACGACAAT TACATACCCT GTACTTTGGA GCCCGTCTTT
    GGAAAGATGT TTGAGCTGAC CTGCTGTCTG CCTCTTGAGA AAGACCTGAA GATTACACTC
    TATGATTACG ACATGGTGAC TAAAGATGAG AAAATCGGAG AGACCATCAT TGATTTGGAG
    AACCGCTTCT TGTCTCGTCA TGGTGCTCGC TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA
    GGAATTAACC AGTGGCGAGA TCAACTTAAA CCATCTCAGC TACTTCATCG TCTGTGTGAG
    AGGCGAAACT ACAAACTCCC CGTCTACAAG CAGGATCGAA TTTACTTCAG AGGACATGAA
    TACACTGTCG CAGACCTTGA TGAAGGGAAA CCTCTTAACC CTCACTTGGG ACCAGTGATG
    GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG CATGGTTTGG TGCCTGAACA TGTCGAGACG
    CGACCCCTCT ACAGCCCCCT TCAGCCAGAT ATAGAACAGG GGCGACTACA AATGTGGGTG
    GACCTGTTCC CTAAGTCTTT AGGCCCTCCA GGTCCTCCAT TCAACATCAC CCCACGCAAA
    GCTAAAAAGT TCTTCCTGCG CTGTATTATA TGGAACACAA GAGATGTAAT CTTGGATGAT
    GTCAGCGTTA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GCACGGCCAT
    GAGGAGAACA AGCAGAAAAC AGACGTGCAC TACCGATCGC TGGGTGGTGA GGGTAACTTT
    AACTGGCGCT TCCTCTTCCC CTTCCACTAT TTACCTGCGG AACAACTGTG CACCATTGAT
    AAAAAGGAGC ACTTTTGGAG TCTGGATAAA GTTGAAACGA AGCTTCCTCC TAAACTTACT
    ATCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTCGGCTC TTTGGAGATG
    GATCTGAACC ACATGCTGAG TCCTGCGAAG TCTCCAGAGA AGTGTGGTCT TGAGATGCTC
    ACTCAGTCCC AGGACAAACT GGTCTCTTTG TTTGAGCAGA AAACGGTGAA AGGCTGGTGG
    CCGTGTGTCT GTGACAAAGA AGACAAGAAG ATACTTGCTG GAAAGGTGGA GATGTCTCTG
    GAAATAGTCT GTGAACAGGA GCAGGATGAG AGGCCTGCAG GACTCGGCCG AGATGAGCCC
    AACATGAATC CCCATCTAGA GGAGCCTCAG CGTCCCGAGA CATCATTCTT ATGGTTCTCG
    TCCCCATATA AGACGTTTAG GTTCATTGTG TGGAGGAGGT TTAAGTGGCT TATTCTGGGC
    CTCTTTATAC TCTTTTTCAT TATTCTCTTC CTTGGAGTAT TTCTCTACGC CTTCCCTAAC
    TATGCTGCTA TGAAGATGGT TGGACCCTTC AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333235.1
    CDS307..6603
    Translation

    Target ORF information:

    RefSeq Version XM_021477560.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA
    ATCCCTGGAC AGGATGAATT TATGGACTGG TGGAGCAAGT TTTATGCCTC CATGGGTGAG
    AGGACCAAGT GTGGGAGTTA TCTGGAGAAA GGGTTCGATA TGCTGCAGGT GTATGACAGA
    GAACTGGAGA AGACTGAAGG CTTTGAGCAA TTTGCTGATT TCTGTCAAAC GTTTAAACTT
    TACCGTGGAA GAACCCAGAA TGACAGCGAA GATCCTTCCG TTGTTGGAGA GTTCAAGGGT
    TCGTTTAAGA TCTATCCATT GCCTGATGAT CCCTCTATGC CTGTACCCAC ACGTCAGTTT
    CGCAAACTCC CCCCGAACGG TATAGAGGAG TGCTTGGTAC GCATCTACAT CATCCAGGCT
    TTTGGGTTAC AGCCCAAAGA TGCCAATGGC AAGTGTGACC CCTATGTTAA GATCACGCTT
    GGCAAGAAAT CAGTCGATGA CCACGACAAT TACATACCCT GTACTTTGGA GCCCGTCTTT
    GGAAAGATGT TTGAGCTGAC CTGCTGTCTG CCTCTTGAGA AAGACCTGAA GATTACACTC
    TATGATTACG ACATGGTGAC TAAAGATGAG AAAATCGGAG AGACCATCAT TGATTTGGAG
    AACCGCTTCT TGTCTCGTCA TGGTGCTCGC TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA
    GGAATTAACC AGTGGCGAGA TCAACTTAAA CCATCTCAGC TACTTCATCG TCTGTGTGAG
    AGGCGAAACT ACAAACTCCC CGTCTACAAG CAGGATCGAA TTTACTTCAG AGGACATGAA
    TACACTGTCG CAGACCTTGA TGAAGGGAAA CCTCTTAACC CTCACTTGGG ACCAGTGATG
    GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG CATGGTTTGG TGCCTGAACA TGTCGAGACG
    CGACCCCTCT ACAGCCCCCT TCAGCCAGAT ATAGAACAGG GGCGACTACA AATGTGGGTG
    GACCTGTTCC CTAAGTCTTT AGGCCCTCCA GGTCCTCCAT TCAACATCAC CCCACGCAAA
    GCTAAAAAGT TCTTCCTGCG CTGTATTATA TGGAACACAA GAGATGTAAT CTTGGATGAT
    GTCAGCGTTA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GCACGGCCAT
    GAGGAGAACA AGCAGAAAAC AGACGTGCAC TACCGATCGC TGGGTGGTGA GGGTAACTTT
    AACTGGCGCT TCCTCTTCCC CTTCCACTAT TTACCTGCGG AACAACTGTG CACCATTGAT
    AAAAAGGAGC ACTTTTGGAG TCTGGATAAA GTTGAAACGA AGCTTCCTCC TAAACTTACT
    ATCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTCGGCTC TTTGGAGATG
    GATCTGAACC ACATGCTGAG TCCTGCGAAG TCTCCAGAGA AGTGTGGTCT TGAGATGCTC
    ACTCAGTCCC AGGACAAACT GGTCTCTTTG TTTGAGCAGA AAACGGTGAA AGGCTGGTGG
    CCGTGTGTCT GTGACAAAGA AGACAAGAAG ATACTTGCTG GAAAGGTGGA GATGTCTCTG
    GAAATAGTCT GTGAACAGGA GCAGGATGAG AGGCCTGCAG GACTCGGCCG AGATGAGCCC
    AACATGAATC CCCATCTAGA GGAGCCTCAG CGTCCCGAGA CATCATTCTT ATGGTTCTCG
    TCCCCATATA AGACGTTTAG GTTCATTGTG TGGAGGAGGT TTAAGTGGCT TATTCTGGGC
    CTCTTTATAC TCTTTTTCAT TATTCTCTTC CTTGGAGTAT TTCTCTACGC CTTCCCTAAC
    TATGCTGCTA TGAAGATGGT TGGACCCTTC AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43390
    Clone ID Related Accession (Same CDS sequence) XM_021477559.1
    Accession Version XM_021477559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA
    ATCCCTGGAC AGCAGGATGA ATTTATGGAC TGGTGGAGCA AGTTTTATGC CTCCATGGGT
    GAGAGGACCA AGTGTGGGAG TTATCTGGAG AAAGGGTTCG ATATGCTGCA GGTGTATGAC
    AGAGAACTGG AGAAGACTGA AGGCTTTGAG CAATTTGCTG ATTTCTGTCA AACGTTTAAA
    CTTTACCGTG GAAGAACCCA GAATGACAGC GAAGATCCTT CCGTTGTTGG AGAGTTCAAG
    GGTTCGTTTA AGATCTATCC ATTGCCTGAT GATCCCTCTA TGCCTGTACC CACACGTCAG
    TTTCGCAAAC TCCCCCCGAA CGGTATAGAG GAGTGCTTGG TACGCATCTA CATCATCCAG
    GCTTTTGGGT TACAGCCCAA AGATGCCAAT GGCAAGTGTG ACCCCTATGT TAAGATCACG
    CTTGGCAAGA AATCAGTCGA TGACCACGAC AATTACATAC CCTGTACTTT GGAGCCCGTC
    TTTGGAAAGA TGTTTGAGCT GACCTGCTGT CTGCCTCTTG AGAAAGACCT GAAGATTACA
    CTCTATGATT ACGACATGGT GACTAAAGAT GAGAAAATCG GAGAGACCAT CATTGATTTG
    GAGAACCGCT TCTTGTCTCG TCATGGTGCT CGCTGCGGCC TTCCGCAGTC CTACTGTCTG
    TCAGGAATTA ACCAGTGGCG AGATCAACTT AAACCATCTC AGCTACTTCA TCGTCTGTGT
    GAGAGGCGAA ACTACAAACT CCCCGTCTAC AAGCAGGATC GAATTTACTT CAGAGGACAT
    GAATACACTG TCGCAGACCT TGATGAAGGG AAACCTCTTA ACCCTCACTT GGGACCAGTG
    ATGGAGCGTC TGGCTCTGTT GGTGTTGAGG AGGCATGGTT TGGTGCCTGA ACATGTCGAG
    ACGCGACCCC TCTACAGCCC CCTTCAGCCA GATATAGAAC AGGGGCGACT ACAAATGTGG
    GTGGACCTGT TCCCTAAGTC TTTAGGCCCT CCAGGTCCTC CATTCAACAT CACCCCACGC
    AAAGCTAAAA AGTTCTTCCT GCGCTGTATT ATATGGAACA CAAGAGATGT AATCTTGGAT
    GATGTCAGCG TTACCGGGGA GAAGATGAGT GACATTTATG TGAAAGGCTG GATGCACGGC
    CATGAGGAGA ACAAGCAGAA AACAGACGTG CACTACCGAT CGCTGGGTGG TGAGGGTAAC
    TTTAACTGGC GCTTCCTCTT CCCCTTCCAC TATTTACCTG CGGAACAACT GTGCACCATT
    GATAAAAAGG AGCACTTTTG GAGTCTGGAT AAAGTTGAAA CGAAGCTTCC TCCTAAACTT
    ACTATCCAGA TTTGGGACAA TGACAAGTTC TCCTTTGATG ATTTCCTCGG CTCTTTGGAG
    ATGGATCTGA ACCACATGCT GAGTCCTGCG AAGTCTCCAG AGAAGTGTGG TCTTGAGATG
    CTCACTCAGT CCCAGGACAA ACTGGTCTCT TTGTTTGAGC AGAAAACGGT GAAAGGCTGG
    TGGCCGTGTG TCTGTGACAA AGAAGACAAG AAGATACTTG CTGGAAAGGT GGAGATGTCT
    CTGGAAATAG TCTGTGAACA GGAGCAGGAT GAGAGGCCTG CAGGACTCGG CCGAGATGAG
    CCCAACATGA ATCCCCATCT AGAGGAGCCT CAGCGTCCCG AGACATCATT CTTATGGTTC
    TCGTCCCCAT ATAAGACGTT TAGGTTCATT GTGTGGAGGA GGTTTAAGTG GCTTATTCTG
    GGCCTCTTTA TACTCTTTTT CATTATTCTC TTCCTTGGAG TATTTCTCTA CGCCTTCCCT
    AACTATGCTG CTATGAAGAT GGTTGGACCC TTCAGTGGAC TAGGAAAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333234.1
    CDS307..6606
    Translation

    Target ORF information:

    RefSeq Version XM_021477559.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA
    ATCCCTGGAC AGCAGGATGA ATTTATGGAC TGGTGGAGCA AGTTTTATGC CTCCATGGGT
    GAGAGGACCA AGTGTGGGAG TTATCTGGAG AAAGGGTTCG ATATGCTGCA GGTGTATGAC
    AGAGAACTGG AGAAGACTGA AGGCTTTGAG CAATTTGCTG ATTTCTGTCA AACGTTTAAA
    CTTTACCGTG GAAGAACCCA GAATGACAGC GAAGATCCTT CCGTTGTTGG AGAGTTCAAG
    GGTTCGTTTA AGATCTATCC ATTGCCTGAT GATCCCTCTA TGCCTGTACC CACACGTCAG
    TTTCGCAAAC TCCCCCCGAA CGGTATAGAG GAGTGCTTGG TACGCATCTA CATCATCCAG
    GCTTTTGGGT TACAGCCCAA AGATGCCAAT GGCAAGTGTG ACCCCTATGT TAAGATCACG
    CTTGGCAAGA AATCAGTCGA TGACCACGAC AATTACATAC CCTGTACTTT GGAGCCCGTC
    TTTGGAAAGA TGTTTGAGCT GACCTGCTGT CTGCCTCTTG AGAAAGACCT GAAGATTACA
    CTCTATGATT ACGACATGGT GACTAAAGAT GAGAAAATCG GAGAGACCAT CATTGATTTG
    GAGAACCGCT TCTTGTCTCG TCATGGTGCT CGCTGCGGCC TTCCGCAGTC CTACTGTCTG
    TCAGGAATTA ACCAGTGGCG AGATCAACTT AAACCATCTC AGCTACTTCA TCGTCTGTGT
    GAGAGGCGAA ACTACAAACT CCCCGTCTAC AAGCAGGATC GAATTTACTT CAGAGGACAT
    GAATACACTG TCGCAGACCT TGATGAAGGG AAACCTCTTA ACCCTCACTT GGGACCAGTG
    ATGGAGCGTC TGGCTCTGTT GGTGTTGAGG AGGCATGGTT TGGTGCCTGA ACATGTCGAG
    ACGCGACCCC TCTACAGCCC CCTTCAGCCA GATATAGAAC AGGGGCGACT ACAAATGTGG
    GTGGACCTGT TCCCTAAGTC TTTAGGCCCT CCAGGTCCTC CATTCAACAT CACCCCACGC
    AAAGCTAAAA AGTTCTTCCT GCGCTGTATT ATATGGAACA CAAGAGATGT AATCTTGGAT
    GATGTCAGCG TTACCGGGGA GAAGATGAGT GACATTTATG TGAAAGGCTG GATGCACGGC
    CATGAGGAGA ACAAGCAGAA AACAGACGTG CACTACCGAT CGCTGGGTGG TGAGGGTAAC
    TTTAACTGGC GCTTCCTCTT CCCCTTCCAC TATTTACCTG CGGAACAACT GTGCACCATT
    GATAAAAAGG AGCACTTTTG GAGTCTGGAT AAAGTTGAAA CGAAGCTTCC TCCTAAACTT
    ACTATCCAGA TTTGGGACAA TGACAAGTTC TCCTTTGATG ATTTCCTCGG CTCTTTGGAG
    ATGGATCTGA ACCACATGCT GAGTCCTGCG AAGTCTCCAG AGAAGTGTGG TCTTGAGATG
    CTCACTCAGT CCCAGGACAA ACTGGTCTCT TTGTTTGAGC AGAAAACGGT GAAAGGCTGG
    TGGCCGTGTG TCTGTGACAA AGAAGACAAG AAGATACTTG CTGGAAAGGT GGAGATGTCT
    CTGGAAATAG TCTGTGAACA GGAGCAGGAT GAGAGGCCTG CAGGACTCGG CCGAGATGAG
    CCCAACATGA ATCCCCATCT AGAGGAGCCT CAGCGTCCCG AGACATCATT CTTATGGTTC
    TCGTCCCCAT ATAAGACGTT TAGGTTCATT GTGTGGAGGA GGTTTAAGTG GCTTATTCTG
    GGCCTCTTTA TACTCTTTTT CATTATTCTC TTCCTTGGAG TATTTCTCTA CGCCTTCCCT
    AACTATGCTG CTATGAAGAT GGTTGGACCC TTCAGTGGAC TAGGAAAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59639
    Clone ID Related Accession (Same CDS sequence) XM_017356879.1 , XM_017356879.2
    Accession Version XM_017356879.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dysferlin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG
    TGGAGCAAGT TTTATGCCTC CATGGGTGAG AGGACCAAGT GTGGGAGTTA TCTGGAGAAA
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT
    CCCTCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC
    AAGTGTGACC CCTATGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC
    TACCGATCGC TGGGTGGTGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG
    TTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCTTTTTCAT TATTCTCTTC
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212368.1
    CDS304..6570
    Translation

    Target ORF information:

    RefSeq Version XM_017356879.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG
    TGGAGCAAGT TTTATGCCTC CATGGGTGAG AGGACCAAGT GTGGGAGTTA TCTGGAGAAA
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT
    CCCTCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC
    AAGTGTGACC CCTATGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC
    TACCGATCGC TGGGTGGTGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG
    TTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCTTTTTCAT TATTCTCTTC
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59639
    Clone ID Related Accession (Same CDS sequence) XM_017356879.1 , XM_017356879.2
    Accession Version XM_017356879.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356879.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG
    TGGAGCAAGT TTTATGCCTC CATGGGTGAG AGGACCAAGT GTGGGAGTTA TCTGGAGAAA
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT
    CCCTCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC
    AAGTGTGACC CCTATGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC
    TACCGATCGC TGGGTGGTGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG
    TTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCTTTTTCAT TATTCTCTTC
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212368.1
    CDS307..6573
    Translation

    Target ORF information:

    RefSeq Version XM_017356879.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356879.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG
    TGGAGCAAGT TTTATGCCTC CATGGGTGAG AGGACCAAGT GTGGGAGTTA TCTGGAGAAA
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT
    CCCTCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC
    AAGTGTGACC CCTATGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC
    TACCGATCGC TGGGTGGTGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG
    TTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCTTTTTCAT TATTCTCTTC
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa35448
    Clone ID Related Accession (Same CDS sequence) NM_001257193.1
    Accession Version NM_001257193.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Danio rerio(zebrafish)
    Product dysferlin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FR725960.1. On Mar 18, 2012 this sequence version replaced XM_684324.4. ##Evidence-Data-START## Transcript exon combination :: FR725960.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAGAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG GTAGCCTTTT TCCGACTTGT GGAAAAAATA
    TTCAATAATT TTGAAAAAAG CTTATTAATT GTGGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CGAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GGTGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGGTAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA
    AACAGTCCGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG
    GGGTACACAG ATGTGAATGG AGAAAAGGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGC GAGGATCTGA AGCGTGCAGT GGATGATCAA
    GGGTGGGAAT ATGGAATAAC CCTCCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CAGGGCCGGC GGCCATCTTT
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT
    ACAACGTTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTG CATCAGAGTC AGAAGACGGT GACTGTTCGA
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA
    GACCATCTTG TGACTGAGAG GAGTCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG
    AACCCTAATT TTGACGTCAA CATCCTGTCC ATTGATGTCC GATTGCCTAA GGAAGAGCTC
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG
    TGGAGCAAGT TTTATGCCTC CATGGGTGAA AGGACCAAGT GTGGGAGTTA TCTGGAGAAA
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT
    CCCCCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC
    AAGTGTGACC CCTACGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC
    TACCGATCGC TGGGTGGAGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG
    CTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCCTTTTCAT TATTCTCTTC
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244122.1
    CDS304..6570
    Translation

    Target ORF information:

    RefSeq Version NM_001257193.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001257193.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA
    CCCAACTTTA ACCAGGGACT AAAGAAAGAG AAACGCCAAA GCAACAAATC CCCCCTGTCC
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT
    AAAAAGGGCA ATAATCCATT TTTTGATGAG GTAGCCTTTT TCCGACTTGT GGAAAAAATA
    TTCAATAATT TTGAAAAAAG CTTATTAATT GTGGTATTTG ATTCCAAGTC ACTACGGAAA
    GACTCTGTCA TTGGAGAATT CGAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG
    GGTGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGGTAAGTAG TAAGATTCTG
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA
    AACAGTCCGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG
    GGGTACACAG ATGTGAATGG AGAAAAGGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGC GAGGATCTGA AGCGTGCAGT GGATGATCAA
    GGGTGGGAAT ATGGAATAAC CCTCCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CAGGGCCGGC GGCCATCTTT
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT
    ACAACGTTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTG CATCAGAGTC AGAAGACGGT GACTGTTCGA
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA
    GACCATCTTG TGACTGAGAG GAGTCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG
    AACCCTAATT TTGACGTCAA CATCCTGTCC ATTGATGTCC GATTGCCTAA GGAAGAGCTC
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG
    TGGAGCAAGT TTTATGCCTC CATGGGTGAA AGGACCAAGT GTGGGAGTTA TCTGGAGAAA
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT
    CCCCCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC
    AAGTGTGACC CCTACGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC
    TACCGATCGC TGGGTGGAGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG
    CTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCCTTTTCAT TATTCTCTTC
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12912
    Clone ID Related Accession (Same CDS sequence) XM_005172291.3 , XM_005172291.4
    Accession Version XM_005172291.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dysferlin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172291.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT
    GGACAGGCAG ATGGCACCAG CTCTGCCATT ATTAACATCT CGCCTTCTCA GTCCAGCCTT
    CATCAGGATG AATTTATGGA CTGGTGGAGC AAGTTTTATG CCTCCATGGG TGAGAGGACC
    AAGTGTGGGA GTTATCTGGA GAAAGGGTTC GATATGCTGC AGGTGTATGA CAGAGAACTG
    GAGAAGACTG AAGGCTTTGA GCAATTTGCT GATTTCTGTC AAACGTTTAA ACTTTACCGT
    GGAAGAACCC AGAATGACAG CGAAGATCCT TCCGTTGTTG GAGAGTTCAA GGGTTCGTTT
    AAGATCTATC CATTGCCTGA TGATCCCTCT ATGCCTGTAC CCACACGTCA GTTTCGCAAA
    CTCCCCCCGA ACGGTATAGA GGAGTGCTTG GTACGCATCT ACATCATCCA GGCTTTTGGG
    TTACAGCCCA AAGATGCCAA TGGCAAGTGT GACCCCTATG TTAAGATCAC GCTTGGCAAG
    AAATCAGTCG ATGACCACGA CAATTACATA CCCTGTACTT TGGAGCCCGT CTTTGGAAAG
    ATGTTTGAGC TGACCTGCTG TCTGCCTCTT GAGAAAGACC TGAAGATTAC ACTCTATGAT
    TACGACATGG TGACTAAAGA TGAGAAAATC GGAGAGACCA TCATTGATTT GGAGAACCGC
    TTCTTGTCTC GTCATGGTGC TCGCTGCGGC CTTCCGCAGT CCTACTGTCT GTCAGGAATT
    AACCAGTGGC GAGATCAACT TAAACCATCT CAGCTACTTC ATCGTCTGTG TGAGAGGCGA
    AACTACAAAC TCCCCGTCTA CAAGCAGGAT CGAATTTACT TCAGAGGACA TGAATACACT
    GTCGCAGACC TTGATGAAGG GAAACCTCTT AACCCTCACT TGGGACCAGT GATGGAGCGT
    CTGGCTCTGT TGGTGTTGAG GAGGCATGGT TTGGTGCCTG AACATGTCGA GACGCGACCC
    CTCTACAGCC CCCTTCAGCC AGATATAGAA CAGGGGCGAC TACAAATGTG GGTGGACCTG
    TTCCCTAAGT CTTTAGGCCC TCCAGGTCCT CCATTCAACA TCACCCCACG CAAAGCTAAA
    AAGTTCTTCC TGCGCTGTAT TATATGGAAC ACAAGAGATG TAATCTTGGA TGATGTCAGC
    GTTACCGGGG AGAAGATGAG TGACATTTAT GTGAAAGGCT GGATGCACGG CCATGAGGAG
    AACAAGCAGA AAACAGACGT GCACTACCGA TCGCTGGGTG GTGAGGGTAA CTTTAACTGG
    CGCTTCCTCT TCCCCTTCCA CTATTTACCT GCGGAACAAC TGTGCACCAT TGATAAAAAG
    GAGCACTTTT GGAGTCTGGA TAAAGTTGAA ACGAAGCTTC CTCCTAAACT TACTATCCAG
    ATTTGGGACA ATGACAAGTT CTCCTTTGAT GATTTCCTCG GCTCTTTGGA GATGGATCTG
    AACCACATGC TGAGTCCTGC GAAGTCTCCA GAGAAGTGTG GTCTTGAGAT GCTCACTCAG
    TCCCAGGACA AACTGGTCTC TTTGTTTGAG CAGAAAACGG TGAAAGGCTG GTGGCCGTGT
    GTCTGTGACA AAGAAGACAA GAAGATACTT GCTGGAAAGG TGGAGATGTC TCTGGAAATA
    GTCTGTGAAC AGGAGCAGGA TGAGAGGCCT GCAGGACTCG GCCGAGATGA GCCCAACATG
    AATCCCCATC TAGAGGAGCC TCAGCGTCCC GAGACATCAT TCTTATGGTT CTCGTCCCCA
    TATAAGACGT TTAGGTTCAT TGTGTGGAGG AGGTTTAAGT GGCTTATTCT GGGCCTCTTT
    ATACTCTTTT TCATTATTCT CTTCCTTGGA GTATTTCTCT ACGCCTTCCC TAACTATGCT
    GCTATGAAGA TGGTTGGACC CTTCAGTGGA CTAGGAAAGG GAACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172348.1
    CDS283..6633
    Translation

    Target ORF information:

    RefSeq Version XM_005172291.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005172291.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT
    GGACAGGCAG ATGGCACCAG CTCTGCCATT ATTAACATCT CGCCTTCTCA GTCCAGCCTT
    CATCAGGATG AATTTATGGA CTGGTGGAGC AAGTTTTATG CCTCCATGGG TGAGAGGACC
    AAGTGTGGGA GTTATCTGGA GAAAGGGTTC GATATGCTGC AGGTGTATGA CAGAGAACTG
    GAGAAGACTG AAGGCTTTGA GCAATTTGCT GATTTCTGTC AAACGTTTAA ACTTTACCGT
    GGAAGAACCC AGAATGACAG CGAAGATCCT TCCGTTGTTG GAGAGTTCAA GGGTTCGTTT
    AAGATCTATC CATTGCCTGA TGATCCCTCT ATGCCTGTAC CCACACGTCA GTTTCGCAAA
    CTCCCCCCGA ACGGTATAGA GGAGTGCTTG GTACGCATCT ACATCATCCA GGCTTTTGGG
    TTACAGCCCA AAGATGCCAA TGGCAAGTGT GACCCCTATG TTAAGATCAC GCTTGGCAAG
    AAATCAGTCG ATGACCACGA CAATTACATA CCCTGTACTT TGGAGCCCGT CTTTGGAAAG
    ATGTTTGAGC TGACCTGCTG TCTGCCTCTT GAGAAAGACC TGAAGATTAC ACTCTATGAT
    TACGACATGG TGACTAAAGA TGAGAAAATC GGAGAGACCA TCATTGATTT GGAGAACCGC
    TTCTTGTCTC GTCATGGTGC TCGCTGCGGC CTTCCGCAGT CCTACTGTCT GTCAGGAATT
    AACCAGTGGC GAGATCAACT TAAACCATCT CAGCTACTTC ATCGTCTGTG TGAGAGGCGA
    AACTACAAAC TCCCCGTCTA CAAGCAGGAT CGAATTTACT TCAGAGGACA TGAATACACT
    GTCGCAGACC TTGATGAAGG GAAACCTCTT AACCCTCACT TGGGACCAGT GATGGAGCGT
    CTGGCTCTGT TGGTGTTGAG GAGGCATGGT TTGGTGCCTG AACATGTCGA GACGCGACCC
    CTCTACAGCC CCCTTCAGCC AGATATAGAA CAGGGGCGAC TACAAATGTG GGTGGACCTG
    TTCCCTAAGT CTTTAGGCCC TCCAGGTCCT CCATTCAACA TCACCCCACG CAAAGCTAAA
    AAGTTCTTCC TGCGCTGTAT TATATGGAAC ACAAGAGATG TAATCTTGGA TGATGTCAGC
    GTTACCGGGG AGAAGATGAG TGACATTTAT GTGAAAGGCT GGATGCACGG CCATGAGGAG
    AACAAGCAGA AAACAGACGT GCACTACCGA TCGCTGGGTG GTGAGGGTAA CTTTAACTGG
    CGCTTCCTCT TCCCCTTCCA CTATTTACCT GCGGAACAAC TGTGCACCAT TGATAAAAAG
    GAGCACTTTT GGAGTCTGGA TAAAGTTGAA ACGAAGCTTC CTCCTAAACT TACTATCCAG
    ATTTGGGACA ATGACAAGTT CTCCTTTGAT GATTTCCTCG GCTCTTTGGA GATGGATCTG
    AACCACATGC TGAGTCCTGC GAAGTCTCCA GAGAAGTGTG GTCTTGAGAT GCTCACTCAG
    TCCCAGGACA AACTGGTCTC TTTGTTTGAG CAGAAAACGG TGAAAGGCTG GTGGCCGTGT
    GTCTGTGACA AAGAAGACAA GAAGATACTT GCTGGAAAGG TGGAGATGTC TCTGGAAATA
    GTCTGTGAAC AGGAGCAGGA TGAGAGGCCT GCAGGACTCG GCCGAGATGA GCCCAACATG
    AATCCCCATC TAGAGGAGCC TCAGCGTCCC GAGACATCAT TCTTATGGTT CTCGTCCCCA
    TATAAGACGT TTAGGTTCAT TGTGTGGAGG AGGTTTAAGT GGCTTATTCT GGGCCTCTTT
    ATACTCTTTT TCATTATTCT CTTCCTTGGA GTATTTCTCT ACGCCTTCCC TAACTATGCT
    GCTATGAAGA TGGTTGGACC CTTCAGTGGA CTAGGAAAGG GAACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12912
    Clone ID Related Accession (Same CDS sequence) XM_005172291.3 , XM_005172291.4
    Accession Version XM_005172291.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172291.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT
    GGACAGGCAG ATGGCACCAG CTCTGCCATT ATTAACATCT CGCCTTCTCA GTCCAGCCTT
    CATCAGGATG AATTTATGGA CTGGTGGAGC AAGTTTTATG CCTCCATGGG TGAGAGGACC
    AAGTGTGGGA GTTATCTGGA GAAAGGGTTC GATATGCTGC AGGTGTATGA CAGAGAACTG
    GAGAAGACTG AAGGCTTTGA GCAATTTGCT GATTTCTGTC AAACGTTTAA ACTTTACCGT
    GGAAGAACCC AGAATGACAG CGAAGATCCT TCCGTTGTTG GAGAGTTCAA GGGTTCGTTT
    AAGATCTATC CATTGCCTGA TGATCCCTCT ATGCCTGTAC CCACACGTCA GTTTCGCAAA
    CTCCCCCCGA ACGGTATAGA GGAGTGCTTG GTACGCATCT ACATCATCCA GGCTTTTGGG
    TTACAGCCCA AAGATGCCAA TGGCAAGTGT GACCCCTATG TTAAGATCAC GCTTGGCAAG
    AAATCAGTCG ATGACCACGA CAATTACATA CCCTGTACTT TGGAGCCCGT CTTTGGAAAG
    ATGTTTGAGC TGACCTGCTG TCTGCCTCTT GAGAAAGACC TGAAGATTAC ACTCTATGAT
    TACGACATGG TGACTAAAGA TGAGAAAATC GGAGAGACCA TCATTGATTT GGAGAACCGC
    TTCTTGTCTC GTCATGGTGC TCGCTGCGGC CTTCCGCAGT CCTACTGTCT GTCAGGAATT
    AACCAGTGGC GAGATCAACT TAAACCATCT CAGCTACTTC ATCGTCTGTG TGAGAGGCGA
    AACTACAAAC TCCCCGTCTA CAAGCAGGAT CGAATTTACT TCAGAGGACA TGAATACACT
    GTCGCAGACC TTGATGAAGG GAAACCTCTT AACCCTCACT TGGGACCAGT GATGGAGCGT
    CTGGCTCTGT TGGTGTTGAG GAGGCATGGT TTGGTGCCTG AACATGTCGA GACGCGACCC
    CTCTACAGCC CCCTTCAGCC AGATATAGAA CAGGGGCGAC TACAAATGTG GGTGGACCTG
    TTCCCTAAGT CTTTAGGCCC TCCAGGTCCT CCATTCAACA TCACCCCACG CAAAGCTAAA
    AAGTTCTTCC TGCGCTGTAT TATATGGAAC ACAAGAGATG TAATCTTGGA TGATGTCAGC
    GTTACCGGGG AGAAGATGAG TGACATTTAT GTGAAAGGCT GGATGCACGG CCATGAGGAG
    AACAAGCAGA AAACAGACGT GCACTACCGA TCGCTGGGTG GTGAGGGTAA CTTTAACTGG
    CGCTTCCTCT TCCCCTTCCA CTATTTACCT GCGGAACAAC TGTGCACCAT TGATAAAAAG
    GAGCACTTTT GGAGTCTGGA TAAAGTTGAA ACGAAGCTTC CTCCTAAACT TACTATCCAG
    ATTTGGGACA ATGACAAGTT CTCCTTTGAT GATTTCCTCG GCTCTTTGGA GATGGATCTG
    AACCACATGC TGAGTCCTGC GAAGTCTCCA GAGAAGTGTG GTCTTGAGAT GCTCACTCAG
    TCCCAGGACA AACTGGTCTC TTTGTTTGAG CAGAAAACGG TGAAAGGCTG GTGGCCGTGT
    GTCTGTGACA AAGAAGACAA GAAGATACTT GCTGGAAAGG TGGAGATGTC TCTGGAAATA
    GTCTGTGAAC AGGAGCAGGA TGAGAGGCCT GCAGGACTCG GCCGAGATGA GCCCAACATG
    AATCCCCATC TAGAGGAGCC TCAGCGTCCC GAGACATCAT TCTTATGGTT CTCGTCCCCA
    TATAAGACGT TTAGGTTCAT TGTGTGGAGG AGGTTTAAGT GGCTTATTCT GGGCCTCTTT
    ATACTCTTTT TCATTATTCT CTTCCTTGGA GTATTTCTCT ACGCCTTCCC TAACTATGCT
    GCTATGAAGA TGGTTGGACC CTTCAGTGGA CTAGGAAAGG GAACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172348.1
    CDS358..6708
    Translation

    Target ORF information:

    RefSeq Version XM_005172291.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005172291.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT
    GGACAGGCAG ATGGCACCAG CTCTGCCATT ATTAACATCT CGCCTTCTCA GTCCAGCCTT
    CATCAGGATG AATTTATGGA CTGGTGGAGC AAGTTTTATG CCTCCATGGG TGAGAGGACC
    AAGTGTGGGA GTTATCTGGA GAAAGGGTTC GATATGCTGC AGGTGTATGA CAGAGAACTG
    GAGAAGACTG AAGGCTTTGA GCAATTTGCT GATTTCTGTC AAACGTTTAA ACTTTACCGT
    GGAAGAACCC AGAATGACAG CGAAGATCCT TCCGTTGTTG GAGAGTTCAA GGGTTCGTTT
    AAGATCTATC CATTGCCTGA TGATCCCTCT ATGCCTGTAC CCACACGTCA GTTTCGCAAA
    CTCCCCCCGA ACGGTATAGA GGAGTGCTTG GTACGCATCT ACATCATCCA GGCTTTTGGG
    TTACAGCCCA AAGATGCCAA TGGCAAGTGT GACCCCTATG TTAAGATCAC GCTTGGCAAG
    AAATCAGTCG ATGACCACGA CAATTACATA CCCTGTACTT TGGAGCCCGT CTTTGGAAAG
    ATGTTTGAGC TGACCTGCTG TCTGCCTCTT GAGAAAGACC TGAAGATTAC ACTCTATGAT
    TACGACATGG TGACTAAAGA TGAGAAAATC GGAGAGACCA TCATTGATTT GGAGAACCGC
    TTCTTGTCTC GTCATGGTGC TCGCTGCGGC CTTCCGCAGT CCTACTGTCT GTCAGGAATT
    AACCAGTGGC GAGATCAACT TAAACCATCT CAGCTACTTC ATCGTCTGTG TGAGAGGCGA
    AACTACAAAC TCCCCGTCTA CAAGCAGGAT CGAATTTACT TCAGAGGACA TGAATACACT
    GTCGCAGACC TTGATGAAGG GAAACCTCTT AACCCTCACT TGGGACCAGT GATGGAGCGT
    CTGGCTCTGT TGGTGTTGAG GAGGCATGGT TTGGTGCCTG AACATGTCGA GACGCGACCC
    CTCTACAGCC CCCTTCAGCC AGATATAGAA CAGGGGCGAC TACAAATGTG GGTGGACCTG
    TTCCCTAAGT CTTTAGGCCC TCCAGGTCCT CCATTCAACA TCACCCCACG CAAAGCTAAA
    AAGTTCTTCC TGCGCTGTAT TATATGGAAC ACAAGAGATG TAATCTTGGA TGATGTCAGC
    GTTACCGGGG AGAAGATGAG TGACATTTAT GTGAAAGGCT GGATGCACGG CCATGAGGAG
    AACAAGCAGA AAACAGACGT GCACTACCGA TCGCTGGGTG GTGAGGGTAA CTTTAACTGG
    CGCTTCCTCT TCCCCTTCCA CTATTTACCT GCGGAACAAC TGTGCACCAT TGATAAAAAG
    GAGCACTTTT GGAGTCTGGA TAAAGTTGAA ACGAAGCTTC CTCCTAAACT TACTATCCAG
    ATTTGGGACA ATGACAAGTT CTCCTTTGAT GATTTCCTCG GCTCTTTGGA GATGGATCTG
    AACCACATGC TGAGTCCTGC GAAGTCTCCA GAGAAGTGTG GTCTTGAGAT GCTCACTCAG
    TCCCAGGACA AACTGGTCTC TTTGTTTGAG CAGAAAACGG TGAAAGGCTG GTGGCCGTGT
    GTCTGTGACA AAGAAGACAA GAAGATACTT GCTGGAAAGG TGGAGATGTC TCTGGAAATA
    GTCTGTGAAC AGGAGCAGGA TGAGAGGCCT GCAGGACTCG GCCGAGATGA GCCCAACATG
    AATCCCCATC TAGAGGAGCC TCAGCGTCCC GAGACATCAT TCTTATGGTT CTCGTCCCCA
    TATAAGACGT TTAGGTTCAT TGTGTGGAGG AGGTTTAAGT GGCTTATTCT GGGCCTCTTT
    ATACTCTTTT TCATTATTCT CTTCCTTGGA GTATTTCTCT ACGCCTTCCC TAACTATGCT
    GCTATGAAGA TGGTTGGACC CTTCAGTGGA CTAGGAAAGG GAACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12914
    Clone ID Related Accession (Same CDS sequence) XM_005172293.3 , XM_005172293.4
    Accession Version XM_005172293.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dysferlin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172293.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT
    GGACAGCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172350.1
    CDS283..6576
    Translation

    Target ORF information:

    RefSeq Version XM_005172293.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005172293.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT
    GGACAGCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12914
    Clone ID Related Accession (Same CDS sequence) XM_005172293.3 , XM_005172293.4
    Accession Version XM_005172293.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172293.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT
    GGACAGCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172350.1
    CDS358..6651
    Translation

    Target ORF information:

    RefSeq Version XM_005172293.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005172293.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT
    GGACAGCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.