DYSF gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001130983.1	NP_001124455.1	dysferlin isoform 6
NM_001130978.1	NP_001124450.1	dysferlin isoform 11
NM_001130980.1	NP_001124452.1	dysferlin isoform 13
NM_001130455.2	NP_001123927.1	dysferlin isoform 2
NM_001130980.2	NP_001124452.1	dysferlin isoform 13
NM_001130982.2	NP_001124454.1	dysferlin isoform 7
NM_001130987.2	NP_001124459.1	dysferlin isoform 1
NM_001130455.1	NP_001123927.1	dysferlin isoform 2
NM_003494.3	NP_003485.1	dysferlin isoform 8
NM_003494.4	NP_003485.1	dysferlin isoform 8
NM_001130982.1	NP_001124454.1	dysferlin isoform 7
NM_001130981.2	NP_001124453.1	dysferlin isoform 14
NM_001130978.2	NP_001124450.1	dysferlin isoform 11
NM_001130979.2	NP_001124451.1	dysferlin isoform 12
XM_005264585.5	XP_005264642.1	dysferlin isoform X2
NM_001130987.1	NP_001124459.1	dysferlin isoform 1
NM_001130976.1	NP_001124448.1	dysferlin isoform 9
NM_001130985.1	NP_001124457.1	dysferlin isoform 4
NM_001130984.1	NP_001124456.1	dysferlin isoform 5
NM_001130986.2	NP_001124458.1	dysferlin isoform 3
NM_001130979.1	NP_001124451.1	dysferlin isoform 12
NM_001130986.1	NP_001124458.1	dysferlin isoform 3
NM_001130981.1	NP_001124453.1	dysferlin isoform 14
NM_001130977.1	NP_001124449.1	dysferlin isoform 10
XM_005264585.4	XP_005264642.1	dysferlin isoform X2
NM_001130985.2	NP_001124457.1	dysferlin isoform 4
NM_001130983.2	NP_001124455.1	dysferlin isoform 6
NM_001130977.2	NP_001124449.1	dysferlin isoform 10
NM_001130976.2	NP_001124448.1	dysferlin isoform 9
NM_001130984.2	NP_001124456.1	dysferlin isoform 5
XM_005264584.4	XP_005264641.1	dysferlin isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28599	NM_001130981.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28600	NM_001130984.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28601	NM_001130987.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28604	NM_001130980.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28606	NM_001130977.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28609	NM_001130982.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28611	NM_001130455.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28612	NM_001130978.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28614	NM_001130979.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu28615	NM_001130986.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28616	NM_001130983.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28617	NM_001130985.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28618	NM_003494.4 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28621	NM_001130976.2 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu35620	XM_005264584.4 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu35621	XM_005264585.5 Latest version!	Homo sapiens dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu28604	NM_001130980.1	Homo sapiens dysferlin (DYSF), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28611	NM_001130455.1	Homo sapiens dysferlin (DYSF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28618	NM_003494.3	Homo sapiens dysferlin (DYSF), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28609	NM_001130982.1	Homo sapiens dysferlin (DYSF), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28612	NM_001130978.1	Homo sapiens dysferlin (DYSF), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28601	NM_001130987.1	Homo sapiens dysferlin (DYSF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28614	NM_001130979.1	Homo sapiens dysferlin (DYSF), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu28615	NM_001130986.1	Homo sapiens dysferlin (DYSF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28599	NM_001130981.1	Homo sapiens dysferlin (DYSF), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu35621	XM_005264585.4	Homo sapiens dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu28617	NM_001130985.1	Homo sapiens dysferlin (DYSF), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28616	NM_001130983.1	Homo sapiens dysferlin (DYSF), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28606	NM_001130977.1	Homo sapiens dysferlin (DYSF), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28621	NM_001130976.1	Homo sapiens dysferlin (DYSF), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28600	NM_001130984.1	Homo sapiens dysferlin (DYSF), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
Hide Old Accession Versions >>

DYSF ( NM_001130981.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124453.1 Homo sapiens dysferlin (DYSF), transcript variant 14, mRNA.

Price & Availability↑

CloneID	OHu28599
Clone ID Related Accession (Same CDS sequence)	NM_001130981.2 , NM_001130981.1
Accession Version	NM_001130981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, AI192657.1, EU515160.1, AI128455.1, DB292971.1 and AK074649.1. On May 31, 2019 this sequence version replaced NM_001130981.1. Transcript Variant: This variant (14) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (14) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124453.1
CDS	439..6795
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130981.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130981.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130981.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124453.1 Homo sapiens dysferlin (DYSF), transcript variant 14, mRNA.

Price & Availability↑

CloneID	OHu28599
Clone ID Related Accession (Same CDS sequence)	NM_001130981.2 , NM_001130981.1
Accession Version	NM_001130981.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515160.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (14) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (14) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124453.1
CDS	377..6733
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130981.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130981.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130984.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124456.1 Homo sapiens dysferlin (DYSF), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu28600
Clone ID Related Accession (Same CDS sequence)	NM_001130984.1 , NM_001130984.2
Accession Version	NM_001130984.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515165.1, AF075575.1, DB292971.1, EU515157.1 and AK074649.1. Transcript Variant: This variant (5) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 5 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTGATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGAAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCGTCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG 
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CAAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124456.1
CDS	142..6408
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130984.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130984.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTGATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGAAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCGTCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG 
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CAAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130984.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124456.1 Homo sapiens dysferlin (DYSF), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu28600
Clone ID Related Accession (Same CDS sequence)	NM_001130984.1 , NM_001130984.2
Accession Version	NM_001130984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, EU515165.1, AF075575.1, DB292971.1, EU515157.1 and AK074649.1. On May 31, 2019 this sequence version replaced NM_001130984.1. Transcript Variant: This variant (5) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 5 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTGATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGAAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCGTCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG 
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CAAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124456.1
CDS	145..6411
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130984.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130984.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTGATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGAAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCGTCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG 
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CAAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130987.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124459.1 Homo sapiens dysferlin (DYSF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28601
Clone ID Related Accession (Same CDS sequence)	NM_001130987.2 , NM_001130987.1
Accession Version	NM_001130987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Homo sapiens(human)
Product	dysferlin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, EU515166.1, AF075575.1, DB292971.1, EU515157.1 and AK074649.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001130987.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000410020.8/ ENSP00000386881.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACC TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTG 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGAAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCGTCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCAAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124459.1
CDS	145..6504
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130987.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130987.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACC TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTG 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGAAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCGTCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCAAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130987.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124459.1 Homo sapiens dysferlin (DYSF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28601
Clone ID Related Accession (Same CDS sequence)	NM_001130987.2 , NM_001130987.1
Accession Version	NM_001130987.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	dysferlin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515166.1, AF075575.1, DB292971.1, EU515157.1 and AK074649.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACC TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTG 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGAAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCGTCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCAAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124459.1
CDS	142..6501
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130987.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130987.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACC TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTG 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGAAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCGTCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCAAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130980.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124452.1 Homo sapiens dysferlin (DYSF), transcript variant 13, mRNA.

Price & Availability↑

CloneID	OHu28604
Clone ID Related Accession (Same CDS sequence)	NM_001130980.1 , NM_001130980.2
Accession Version	NM_001130980.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515158.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (13) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (13) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACCTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTGATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GAAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
GTCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCAA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124452.1
CDS	377..6670
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130980.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130980.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACCTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTGATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GAAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
GTCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCAA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130980.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124452.1 Homo sapiens dysferlin (DYSF), transcript variant 13, mRNA.

Price & Availability↑

CloneID	OHu28604
Clone ID Related Accession (Same CDS sequence)	NM_001130980.1 , NM_001130980.2
Accession Version	NM_001130980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, AI192657.1, EU515158.1, AI128455.1, DB292971.1 and AK074649.1. On May 31, 2019 this sequence version replaced NM_001130980.1. Transcript Variant: This variant (13) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (13) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACCTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTGATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GAAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
GTCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCAA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124452.1
CDS	439..6732
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130980.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130980.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACCTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTGATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GAAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
GTCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCAA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130977.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124449.1 Homo sapiens dysferlin (DYSF), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu28606
Clone ID Related Accession (Same CDS sequence)	NM_001130977.1 , NM_001130977.2
Accession Version	NM_001130977.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515159.1, AI739271.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (10) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (10) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124449.1
CDS	377..6640
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130977.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130977.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130977.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124449.1 Homo sapiens dysferlin (DYSF), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu28606
Clone ID Related Accession (Same CDS sequence)	NM_001130977.1 , NM_001130977.2
Accession Version	NM_001130977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, AI192657.1, EU515159.1, AI739271.1, DB292971.1 and AK074649.1. On May 31, 2019 this sequence version replaced NM_001130977.1. Transcript Variant: This variant (10) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (10) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124449.1
CDS	439..6702
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130977.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130977.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130982.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124454.1 Homo sapiens dysferlin (DYSF), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu28609
Clone ID Related Accession (Same CDS sequence)	NM_001130982.2 , NM_001130982.1
Accession Version	NM_001130982.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, EU515161.1, AF075575.1 and DB292971.1. On May 31, 2019 this sequence version replaced NM_001130982.1. Transcript Variant: This variant (7) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 7 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG 
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124454.1
CDS	145..6483
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130982.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130982.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG 
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130982.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124454.1 Homo sapiens dysferlin (DYSF), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu28609
Clone ID Related Accession (Same CDS sequence)	NM_001130982.2 , NM_001130982.1
Accession Version	NM_001130982.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515161.1, AF075575.1 and DB292971.1. Transcript Variant: This variant (7) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 7 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG 
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124454.1
CDS	142..6480
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130982.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130982.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG 
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130455.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123927.1 Homo sapiens dysferlin (DYSF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28611
Clone ID Related Accession (Same CDS sequence)	NM_001130455.2 , NM_001130455.1
Accession Version	NM_001130455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, DQ267935.1, AF075575.1 and DB292971.1. On May 31, 2019 this sequence version replaced NM_001130455.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ267935.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123927.1
CDS	145..6390
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130455.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130455.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130455.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123927.1 Homo sapiens dysferlin (DYSF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28611
Clone ID Related Accession (Same CDS sequence)	NM_001130455.2 , NM_001130455.1
Accession Version	NM_001130455.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ267935.1, AF075575.1 and DB292971.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ267935.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123927.1
CDS	142..6387
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130455.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130455.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130978.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124450.1 Homo sapiens dysferlin (DYSF), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu28612
Clone ID Related Accession (Same CDS sequence)	NM_001130978.1 , NM_001130978.2
Accession Version	NM_001130978.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515157.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (11) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (11) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124450.1
CDS	377..6682
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130978.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130978.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130978.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124450.1 Homo sapiens dysferlin (DYSF), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu28612
Clone ID Related Accession (Same CDS sequence)	NM_001130978.1 , NM_001130978.2
Accession Version	NM_001130978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, AI192657.1, EU515157.1, AI128455.1, DB292971.1 and AK074649.1. On May 31, 2019 this sequence version replaced NM_001130978.1. Transcript Variant: This variant (11) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (11) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124450.1
CDS	439..6744
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130978.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130978.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130979.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124451.1 Homo sapiens dysferlin (DYSF), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu28614
Clone ID Related Accession (Same CDS sequence)	NM_001130979.2 , NM_001130979.1
Accession Version	NM_001130979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, AI192657.1, EU515155.1, AI128455.1, DB292971.1 and AK074649.1. On May 31, 2019 this sequence version replaced NM_001130979.1. Transcript Variant: This variant (12) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (12) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515155.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC 
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACCTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTGATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGAAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCGTCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC AAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124451.1
CDS	439..6774
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130979.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130979.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC 
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACCTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTGATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGAAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCGTCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC AAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130979.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124451.1 Homo sapiens dysferlin (DYSF), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu28614
Clone ID Related Accession (Same CDS sequence)	NM_001130979.2 , NM_001130979.1
Accession Version	NM_001130979.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515155.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (12) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (12) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515155.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC 
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACCTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTGATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGAAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCGTCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC AAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124451.1
CDS	377..6712
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130979.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130979.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC 
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACCTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTGATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGAAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCGTCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC AAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130986.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124458.1 Homo sapiens dysferlin (DYSF), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28615
Clone ID Related Accession (Same CDS sequence)	NM_001130986.2 , NM_001130986.1
Accession Version	NM_001130986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, EU515162.1, AF075575.1 and DB292971.1. On May 31, 2019 this sequence version replaced NM_001130986.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 3 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124458.1
CDS	145..6348
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130986.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130986.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130986.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124458.1 Homo sapiens dysferlin (DYSF), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28615
Clone ID Related Accession (Same CDS sequence)	NM_001130986.2 , NM_001130986.1
Accession Version	NM_001130986.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515162.1, AF075575.1 and DB292971.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 3 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124458.1
CDS	142..6345
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130986.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130986.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130983.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124455.1 Homo sapiens dysferlin (DYSF), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu28616
Clone ID Related Accession (Same CDS sequence)	NM_001130983.1 , NM_001130983.2
Accession Version	NM_001130983.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515163.1, AF075575.1, DB292971.1, EU515157.1 and AK074649.1. Transcript Variant: This variant (6) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 6 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515163.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACCT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTGA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGAAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG 
ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA 
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124455.1
CDS	142..6450
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130983.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130983.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACCT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTGA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGAAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG 
ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA 
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130983.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124455.1 Homo sapiens dysferlin (DYSF), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu28616
Clone ID Related Accession (Same CDS sequence)	NM_001130983.1 , NM_001130983.2
Accession Version	NM_001130983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, EU515163.1, AF075575.1, DB292971.1, EU515157.1 and AK074649.1. On May 31, 2019 this sequence version replaced NM_001130983.1. Transcript Variant: This variant (6) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 6 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515163.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACCT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTGA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGAAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG 
ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA 
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124455.1
CDS	145..6453
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130983.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130983.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG 
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACCT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTGA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGAAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG 
ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA 
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130985.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124457.1 Homo sapiens dysferlin (DYSF), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28617
Clone ID Related Accession (Same CDS sequence)	NM_001130985.1 , NM_001130985.2
Accession Version	NM_001130985.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515164.1, AF075575.1 and DB292971.1. Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 4 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515164.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124457.1
CDS	142..6438
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130985.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130985.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130985.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124457.1 Homo sapiens dysferlin (DYSF), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28617
Clone ID Related Accession (Same CDS sequence)	NM_001130985.1 , NM_001130985.2
Accession Version	NM_001130985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, EU515164.1, AF075575.1 and DB292971.1. On May 31, 2019 this sequence version replaced NM_001130985.1. Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 4 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515164.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124457.1
CDS	145..6441
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130985.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130985.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_003494.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003485.1 Homo sapiens dysferlin (DYSF), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu28618
Clone ID Related Accession (Same CDS sequence)	NM_003494.3 , NM_003494.4
Accession Version	NM_003494.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, AF075575.1, AI128455.1 and DB292971.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2008 this sequence version replaced NM_003494.2. Transcript Variant: This variant (8) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (8) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF075575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACCTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTGATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG AAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCG TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCAAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003485.1
CDS	377..6619
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_003494.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003494.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACCTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTGATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG AAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCG TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCAAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_003494.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003485.1 Homo sapiens dysferlin (DYSF), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu28618
Clone ID Related Accession (Same CDS sequence)	NM_003494.3 , NM_003494.4
Accession Version	NM_003494.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, AI192657.1, AF075575.1, AI128455.1 and DB292971.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_003494.3. Transcript Variant: This variant (8) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (8) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF075575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACCTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTGATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG AAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCG TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCAAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003485.1
CDS	439..6681
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_003494.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003494.4

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACCTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTGATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG AAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCG TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCAAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130976.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124448.1 Homo sapiens dysferlin (DYSF), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu28621
Clone ID Related Accession (Same CDS sequence)	NM_001130976.1 , NM_001130976.2
Accession Version	NM_001130976.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dysferlin isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515156.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (9) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (9) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515156.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
CTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT GATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGAA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCGTC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCAAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124448.1
CDS	377..6577
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130976.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130976.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
CTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT GATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGAA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCGTC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCAAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( NM_001130976.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124448.1 Homo sapiens dysferlin (DYSF), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu28621
Clone ID Related Accession (Same CDS sequence)	NM_001130976.1 , NM_001130976.2
Accession Version	NM_001130976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dysferlin isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104084.5, AI192657.1, EU515156.1, AI128455.1, DB292971.1 and AK074649.1. On May 31, 2019 this sequence version replaced NM_001130976.1. Transcript Variant: This variant (9) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (9) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515156.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
CTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT GATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGAA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCGTC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCAAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124448.1
CDS	439..6639
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	NM_001130976.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130976.2

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG 
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
CTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT GATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGAA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCGTC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCAAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005264584.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264641.1 Homo sapiens dysferlin (DYSF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu35620
Clone ID Related Accession (Same CDS sequence)	XM_005264584.4
Accession Version	XM_005264584.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264584.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG 
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CCTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TGATTGGTGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCCCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGA AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGCTGGA GCCCGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTAAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAGGACGAA AAGATCGGTG AGACGGTCGT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCATAGAG TCAAGGCACC TGTGTACCGG ACAGACCGTG TAATGTTTCA GGATAAAGAA 
TATTCCATTG AAGAGATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCAGCAG CAGGGCCTGG TCCCGGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTATTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGTTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCAGCTG AGCAAGTCTG TACCATTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTG 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCAAGA AGTGCTCCTT GGACCAGCTG 
GATGATGCTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAAATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTTACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264641.1
CDS	157..6558
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005264584.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264584.4

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG 
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CCTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TGATTGGTGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCCCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGA AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGCTGGA GCCCGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTAAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAGGACGAA AAGATCGGTG AGACGGTCGT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCATAGAG TCAAGGCACC TGTGTACCGG ACAGACCGTG TAATGTTTCA GGATAAAGAA 
TATTCCATTG AAGAGATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCAGCAG CAGGGCCTGG TCCCGGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTATTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGTTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCAGCTG AGCAAGTCTG TACCATTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTG 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCAAGA AGTGCTCCTT GGACCAGCTG 
GATGATGCTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAAATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTTACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005264585.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264642.1 Homo sapiens dysferlin (DYSF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu35621
Clone ID Related Accession (Same CDS sequence)	XM_005264585.5 , XM_005264585.4
Accession Version	XM_005264585.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005264585.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC 
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCCACTAACA CGGCTTCTCC TCCATCCAGT 
CCTCATGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264642.1
CDS	846..7244
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005264585.5
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264585.5

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC 
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCCACTAACA CGGCTTCTCC TCCATCCAGT 
CCTCATGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_005264585.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264642.1 Homo sapiens dysferlin (DYSF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu35621
Clone ID Related Accession (Same CDS sequence)	XM_005264585.5 , XM_005264585.4
Accession Version	XM_005264585.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264585.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC 
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCCACTAACA CGGCTTCTCC TCCATCCAGT 
CCTCATGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264642.1
CDS	845..7243
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_005264585.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264585.4

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC 
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCCACTAACA CGGCTTCTCC TCCATCCAGT 
CCTCATGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DYSF
Entrez Gene ID	8291
Full Name	dysferlin
Synonyms	FER1L1,LGMD2B,MMD1
General protein information	Preferred Names dysferlin Names dysferlin dystrophy-associated fer-1-like 1 fer-1-like family member 1 fer-1-like protein 1 limb girdle muscular dystrophy 2B (autosomal recessive)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.2
Summary	The protein encoded by this gene belongs to the ferlin family and is a skeletal muscle protein found associated with the sarcolemma. It is involved in muscle contraction and contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. In addition, the protein encoded by this gene binds caveolin-3, a skeletal muscle membrane protein which is important in the formation of caveolae. Specific mutations in this gene have been shown to cause autosomal recessive limb girdle muscular dystrophy type 2B (LGMD2B) as well as Miyoshi myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2008].
Disorder MIM:	603009
Disorder Html:	Muscular dystrophy, limb-girdle, type 2B, 253601 (3); Myopathy, distal, with anterior tibial onset, 606768 (3); Miyoshi muscular dystrophy 1, 254130 (3)

Homo sapiens (human)	DYSF	NP_001124454.1
	DYSF	XP_002799336.1
Caenorhabditis elegans (roundworm)	fer-1	NP_492337.1
Caenorhabditis elegans (roundworm)	ferl-1	NP_491602.2
Rattus norvegicus (Norway rat)	Dysf	NP_001101339.1
Danio rerio (zebrafish)	dysf	NP_001244122.1
Mus musculus (house mouse)	Dysf	NP_067444.2
Canis lupus familiaris (dog)	DYSF	XP_003432278.1
Bos taurus (cattle)	DYSF	NP_001095960.1
Pan troglodytes (chimpanzee)	DYSF	XP_003309136.1
Gallus gallus (chicken)	DYSF	XP_423126.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DYSF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: